LOCUS       DAOOBB010000001        36553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_1485_length_36553_cov_4.622171, whole genome shotgun sequence.
ACCESSION   DAOOBB010000001 DAOOBB010000000
VERSION     DAOOBB010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 36553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36553
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1485_length_36553_cov_4.622171"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..1258
                     /locus_tag="PLT31_00005"
     CDS             95..1258
                     /locus_tag="PLT31_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HPW93546.1"
                     /translation="MFEIFSSVSEMLVNDTGFQYITGSMVVMWVVSFVLMYLAIVKKF
                     EPLLLLPIAFGALAVNLPTKLFYDGGWTIEGMFTPTAGLYYYISQGIHLELFPPLIFL
                     GVGAMTDFGPLIANPRTLLLGGGAQFGVFATMFCAVAFGGFTLGEAASIGIIGGADGP
                     TSIFTANKLAKHLIGPIAVAAYTYMALVPLIQPPIMRLMTTAKERTIRMKSLRKVSKL
                     ERIVFNIMVMIVCVLVVPDASALIIMLMLGNLMKESGVVERLVKTAQNELMNIVTIFL
                     GTSVGLTMASDIFLRPQTLAIIGMGVVAFGFSTMGGLLLAKIMNVLSPKNPVNPLIGS
                     AGVSAVPMAARVSQVEGAKYDPQNFLLMHAMGPNVAGVIGTAVAAGYMISRLA"
     gene            complement(1350..3065)
                     /locus_tag="PLT31_00010"
     CDS             complement(1350..3065)
                     /locus_tag="PLT31_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HPW93547.1"
                     /translation="MMRNTPLIKKGISSLIILQVILFALSFVVSCSDNKISAGGNTSE
                     VGSPEMLGTLALNNGKQNRASFARVYCIPIDYNSVKEDTSSYYSTIADSMGNFVFDSL
                     PNGLYNFEAFFNDSQGVVYALRESGIHIQKDSTWKVNLTIPTSGSLKIYLSNIKDSIA
                     NLSIVGSSYNSAAKIFEDEIGTYATFTNIPSAYYDSIQIAISSDMIVLNSIFVKEESQ
                     EIYNSNNTIRTHHISLNTAANGIDLQDTLNGFPLFIRLNDIDSLNRESIIKHLNTLKI
                     YQEPASDETSYKVVYDKDSIPSGLWIRLTTLYPQREMQKITLSWEEVEDSNISAVNTV
                     KPFSLSDNFIAAWDFNEDAFPESTIYAAGDNDFQGYVNDITASEGIIGGAFYFNGKTS
                     YIEIKESADYSGFSLVDTSSFTVSFWAFSEDTSTAKFLWGKSEAEYHFKYQAKGQSDA
                     CWMFKEFDESKADEWYESSIVATPETDFKQWVHFTVIKSGDSTAIYKNGKLENVTTGY
                     NQTDEERIAGGSFVIGARKMADGNVDRVFKGMLDELFFQNKVQSEDWAKLIYLNQKPN
                     DYWPK"
     gene            complement(3073..3933)
                     /locus_tag="PLT31_00015"
     CDS             complement(3073..3933)
                     /locus_tag="PLT31_00015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02147 family protein"
                     /protein_id="HPW93548.1"
                     /translation="MKTIKQHEQNGRKSVFDFDNYREYLRNYFEESKALNSRYSLRLF
                     SNKLGFSSKDFILRIMNGEKNLTPSSILKIMNGLQMNEDESSYFEALVLLCQATTEEE
                     RQQYQQRVNSIAATYKFIQQTQLTRAYQYEIYSHWYYSAIRSIIGMFGFDGNYAALGK
                     KLTPPISAEQTRNSVNLLERVGFIKKDRNGNWVLNNAAITTGDKVIQQAFINYHKECL
                     SLASDSITNFSSSERNISSVTLGISEESYKKIVQCINDFRKKISMIANEDEKGGRVFQ
                     MNIQIFPLSK"
     gene            4114..5919
                     /locus_tag="PLT31_00020"
     CDS             4114..5919
                     /locus_tag="PLT31_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HPW93549.1"
                     /translation="MKKNIYKVFLAVASFAIMATAAVDQCKPIGWATTSGRTSTAYNV
                     TGGGNATPITVTNFADLQKYMNDDTPRVLYVKGPIGSGWKGVTGDRLEIKYSNKTIIG
                     SNPGTELKATIHISNNAQNIIIRNIVINGPGSNQDQAWDNLNIEGGAKNIWIDHCEFW
                     DGQDGNADVVKGADNVTFTWCIFGYKIKSSHNLSNLIASSDNEPVSEGKLNVTYAFNW
                     WKAATQRKPRCRYGNIHVMNNLFTGDKNIQSGSIMGISAGVKCYVRAENNHFIDEATP
                     IHKRDGGVTESIGDKFTNCTGSTASLGASFTPPYEYKSYMISAEEVEAVVKANAGATL
                     TSPTQCNSNVTPVSSSSVVASSSSIIASSSSIVASSSSVIASSSSIVASSSSVERPEG
                     FIQIEDGQITGGVLETKNSGFKGPGYVNFDMGGSVVVSVTVEAAGQYELDIYYANGST
                     GNRSLEISAGNSMALIEFGNTSSWTDWKTEKCKLDLSAGENSIKFATVAENDGPNIDQ
                     FSMELLKTTNISDSDNNGSLFTANSQQFHVILNGRNMQFQNKVVSAQVFSIMGLPIKN
                     VQNTNQMNLTDVPSGIYLLKISVDSKTHFQKIKLD"
     gene            5977..9588
                     /locus_tag="PLT31_00025"
     CDS             5977..9588
                     /locus_tag="PLT31_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibro-slime domain-containing protein"
                     /protein_id="HPW93550.1"
                     /translation="MKKINLRLLQKVVFLTIFLISALFAETKTIAFFTPWSNTNAVLF
                     MNGDSVTIMTPLKNYCGWYRAEVEVPASGFNVHFKQTVGLNYVGAEGMTTIPPTIASE
                     ISLDSVALLSDTIWVQGYKNDIPALFSIYPGVLGDCPLKKFPVTLFDWLHGDNGDGDE
                     EGKNGDPINGVSADFGSGGCRGASHSTGIMVGMVEKELGENGLPVPANPFPEDCKIST
                     HLSSWFLPEVIAQDAEGNNYTNMTCRDLYISMDDEGFWLAEVSKDQISEGNEKHKDGM
                     FPLDDFQYLDEAMTVLNPYYDQFKSTDGSGDKHNFGFTMKIQATFEYVPGQYFDFYGD
                     DDVWVFINRKLVVDIGGQHPQKAGAVNLDSLGLVPGDKYDFHIFYAERHTGSSNFRMR
                     TSIDLQVDASLFLTNKRNSNSVDYKIWQVNKKGTFSCGNDINTTELDTTSGAAIFKLT
                     GGNLVGPEILAQGTRYEGIHITSDSTFSIDSAAIIASYALAPGHYFLEVTLKSDPSQM
                     TKIEITIPSYAIPSVAFAKMDWTILGKDVSGDTVQIGNWTNAFYQVNITFLEEWAKVN
                     NYNRKINLSFSDPNIDIFDTIDGQKISSVSLDDNGRATFYVYANAPVAGASLIAKGAA
                     AGVSVWRDLDFVESPIPKVLKAVIKDRNGDGRADSLFVRFDRSLLEKSSLDSIQITFG
                     ESFKVTSQFSLVNEMDVVAVAEKVSPENCTGDVCGFGSRLFTGSNSDVYFGSLNNWFS
                     YKDSSKVTNFYQENNPIEDGVGPIILSAASSRINDANKSISLTFSEAISEESRNNNYM
                     EMFEFICMRSGVNEIPEYPAMQSSSQNTMVFVYSSTDLDVVVPTAGDQIRFNPDNETK
                     DLVGNVPHKNNPWVPITGEQEFSNESPKVITIGEDPYNIIKTDIITQPMIITNLTQNA
                     QEIGDSLGVQGNLIDYDFSKIVIEKTKKEAQTLESFIESRLENITSYDTLITSISEQE
                     ALSQVFADIRTGELNSMYGFSEETIAAVIAGTITEENFNSSISENELLVITKLMQDNI
                     DATRDTVISLSAASQVTQSDLFNAMRNGSLDKELKKAGVSQTLIDAIKNDEVTEFNLE
                     EFSRGSKLVYAEDDVQFFYRTHYYSHLGEYVGGTSNSIKCSDNNVFGEEGCIQNKGKM
                     FLAWNMRSTQGRLVATGVYISLLEIKIIVNGKTSLHQISKKRWGVKRSLINELQLEF"
     gene            9759..10091
                     /locus_tag="PLT31_00030"
     CDS             9759..10091
                     /locus_tag="PLT31_00030"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPW93551.1"
                     /translation="MSPKHKPLAWLSDLPKSPPMSMNARIEIGYLLRLLQSGINVSMP
                     HSRPMPSIGKRCHELRVNDEKCTWRLFYRIDEDAIVVMNWEFKKTEATSKKMIELCQK
                     RLSDYDSI"
     gene            10104..10415
                     /locus_tag="PLT31_00035"
     CDS             10104..10415
                     /locus_tag="PLT31_00035"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPW93552.1"
                     /translation="MEKIKKKKLEAKGWTVGDIDGFLGLDEAEMTIVEMKVALAKALV
                     EKRKSSNVTQVNMAKIIGSSQSRVAKIEKADSSVSLELMIKSFLSLGATKKDIVKVFD
                     K"
     gene            complement(10615..10690)
                     /locus_tag="PLT31_00040"
     tRNA            complement(10615..10690)
                     /locus_tag="PLT31_00040"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10653..10655),aa:His,seq:gtg)
     gene            complement(10745..11113)
                     /locus_tag="PLT31_00045"
     CDS             complement(10745..11113)
                     /locus_tag="PLT31_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93553.1"
                     /translation="MQLPKYKKKKRVKLKVCQEPGCGKEFWGHPIAKYCDDHRDIKQR
                     QKQKKDIENIESKNIIFRHNYTEVMDLPFKCCLDGCDFDFTIKVFPKQFVYPRFCMEH
                     RNDFKRANFIRIMSKKNRDD"
     gene            11222..12073
                     /locus_tag="PLT31_00050"
     CDS             11222..12073
                     /locus_tag="PLT31_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPW93554.1"
                     /translation="MHTILFTLIILLCSALMSCEEGSFLEEKPLKVHFLDVGQGLSVL
                     FEYEGRFALWDTGPDSIGFVDTLKHYGVDSLDWVVLSHWHRDHVGGFLEFPSAIKNRE
                     LFVKKVFFTNDTAYGFVSDSVFSILDEFGLNNDTIYRGDSLIFASGWRFQILWPSKGI
                     NLGGNASSGVLFIRDSLNKMLLMSDLESREEESLLSLYPFLKTDLLQVGHHGSLNSSS
                     IKFLSQILPSEAIISVGEGNSYHHPAQGTLSKLNYVVGDSSFVHRTDLEGTLIWLWHK
                     NIGFWMP"
     gene            12096..13862
                     /locus_tag="PLT31_00055"
     CDS             12096..13862
                     /locus_tag="PLT31_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93555.1"
                     /translation="MIHTTHIPVMGTSYTIDTPIRVAPFGIDSTISLVDNYLLEDLRA
                     YYSRLYNLPYEPILRNEEDGCAKRVQAYLDMVHTIVCRRFEEIRNLPFEKGNEKEKYF
                     TMLPSAHALRKGFESVLKMPLGAERSALEASLNSQMYMGSIDVNLMVKLDYKNVAKDA
                     LRGFAHSKITNGAVVFSAGVNLGLFNDLVKYPDFYRTKDGIKKRIILKISDYRSALTQ
                     GKFLAKKGLEVSEFRVESGLNCGGHAFASQGMLLPLILEELKEKRESLSKTFYPLIQK
                     YYDAQGMDSSVLDPDRDARFTVQGGIGNSGENRRMLEYYKVDGTGWGSAFLMVPEACL
                     LDDYTLEILRDAKREDLYLSDASPIGVPFNNTTRSTANVQRIKNIEAGSSGFPCVNGF
                     LRLNTEFTEEPICTASIQYQKLKIAQIQGSDLSPEEKQNAIKKVQAKECICSHLGNPI
                     RIAFGIVEASKAPVSVCPGPTLAYFKGPYSLVQMMAHIYGDGPSLVPSDRPHLFAEEI
                     RLYTEWFIKESKEATPEYAKWLHQAALNLYENMDYCLNFAKTQEAFPGENLASIGNAI
                     TLYRPQLEKAHEGLKAKHQNLS"
     gene            complement(13840..14589)
                     /locus_tag="PLT31_00060"
     CDS             complement(13840..14589)
                     /locus_tag="PLT31_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HPW93556.1"
                     /translation="MTTPEITSKTFTVRFSDCDHHSRLRVSNLFRFMEESAISDAEQN
                     GFGIWKLMKHGYTYAISRIKIRLHHTPVWGEKLTISTWTKGYYKDKVALKDYLILDAQ
                     GNSIAQGTSSWLLVNLKTGKAENPELSPYPPPIFPNKEALPQMLGILEPGNHPQIVLE
                     KEALYSDLDMNRHVNNCRYTDWVMDAFSLEELKARPLRSIQINFISQIPYQGKVKIVR
                     FENTNHHALLFGVNSEDPSIVHFQARIGFGA"
     gene            complement(14576..15058)
                     /locus_tag="PLT31_00065"
     CDS             complement(14576..15058)
                     /locus_tag="PLT31_00065"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPW93557.1"
                     /translation="MKTESDKKIIDFLAKSYLCEGLSEHQIEELSSFAIMIYLKENEL
                     LFDEGDKTDHFFVVATGKLKALVSSSTGSEMQELRSIHPGDCIGEMAMIEESTHTTRI
                     CAVKASALLSFNSIKLLEYLKKQPETGVQVLLNLSKIISNRLRFLGHPCDLLFINDNS
                     "
     gene            15084..16379
                     /gene="aroA"
                     /locus_tag="PLT31_00070"
     CDS             15084..16379
                     /gene="aroA"
                     /locus_tag="PLT31_00070"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HPW93558.1"
                     /translation="MKSVMFFPSSISLPAFSMFEGVLRLPGSKSLSNRALLLAALAQG
                     ETRLHHLLESDDTRYMCSALEQLGLPIHFLENSSQVTITGSGGPLKAEAGDLYLGNAG
                     TAMRSLCAALTLGKGKFRLSGEERMAERPIKDLVDSLIDMGADIEYEVSEGYPPLLVR
                     ADGLKGGTVHVRGNISSQYLTALLISGPYCKQPLHVIVDGDLISKPYIDLTIDIMRRF
                     GILVDNMNYQEFKVPQGVYVSPGDLDIEGDASSASYPLAGAAITGGTVRVEGVGSSSL
                     QGDVQFAEVLRQMGATVSMGEHWIECTGGELKGVDLDLNHIPDAAMTVAILALFAKGT
                     TTIRNIGSWRVKETDRIAALAAELRKVGATVYTDMTSIQVTPPEKLQEAIIETYNDHR
                     MAMCFSLVALGGVPVKILDPSCVNKTYPNYFDDFLRLAK"
     gene            16376..17950
                     /locus_tag="PLT31_00075"
     CDS             16376..17950
                     /locus_tag="PLT31_00075"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPW93559.1"
                     /translation="MRLVLFLLILLSSSFAAKKVDTLSFWAMDNGPGSAAVLQEMLTR
                     FEVETSIPVVFKELKWESAFDEIQKAFSEGTAPDVLQVGSSWISIFAGKGYLDGMQSF
                     LNEIDSSRFFDEMSKFSSIEGDSNYYAVPWFLDVRAFFGNKAVLDSLGIRDAHMKSFT
                     RARGVMKAIAQNGLKNAEGKEIVPFGLPGKDDWTAPQVIAPWIWSYGGDFLVKQDSLW
                     KSALLDSNTLNGLFHYLTLLKDTTCVPNALFENSVENATRFTNSEQAFLMGTSEIIRK
                     MDVSVEEGGLKEKSISQDGLVLIPMPFGPKGQFTFVGGSHLAISKNAKKDTKKLLQFL
                     VRVDHIDTYTRRIGFLPSDKSVISVWAQDPRYNALIEDLSHGRSFPNLSQWGEIETIL
                     INMSNDIGSIYRTAIENEDRTAEVASIIVRADEKIQNLLSTDSLDSLSSIDSTNSSIR
                     NTDSLLTWVMKILSQKEKEEDFLTVKWFPKGFIIPLPGESWTFRFAIIIGIGVICLFI
                     FFVESKYHLIRRKKKK"
     gene            17952..20156
                     /gene="dacB"
                     /locus_tag="PLT31_00080"
     CDS             17952..20156
                     /gene="dacB"
                     /locus_tag="PLT31_00080"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HPW93560.1"
                     /translation="MKDWVEEKRKIQEPSSLIGCFKKIFFLFSLLFSSQAAFAGFSAQ
                     EFQSYVDSLIPGSRLGMSVRSVKTGNVFLDIRGDEFFTPASTLKTLTTATALHLLPLN
                     YEPQTTLHLEGSIKEKTFSGRLRFYGEGDPNISGRFYTDAFYMMNQMVDSLKALGIDT
                     IRGSLFTDTTYFSGPRRPEHWKNNYYDAWYGAEVVPLGFNDNCTLIRIKPGANVNDTA
                     IITPLPDIGFVTIKNELITTNNRARRWRYSIDKEKPVITISGTIGIKVDSSALVLPVR
                     NPSAYFISAFESALKDAGIIFIQDYSAHSGIEIKQFRFSAAPLLSMLDEINQRSQNLH
                     AEMLFRNVGKIVAGEGSVEGGKKAETLFLNQIGISPSHFKVFDGCGLSPLNKVKPSVI
                     TEMLSYMARSPRSYFYIESFASPGVGTASKRMNSLAMPQQTRLKTGFISEAHALVGYI
                     FTMTGDTLSVSAYLNDTGKNPDNKVKDVLDTIWSRLVEKSNDHYPSLMLAKKLWLQGL
                     SVKGLENRLDYFSKALLNTPYLLGPMGESYLDSIDSKPLIYLDSVDCVTYIEHVLALA
                     TASHEDSVFSQLQRIRYFDGKIGYLYRKHYFVEDFLGENVFASVRIMEGDTTIIRELP
                     KKDFFASKKIKYQLDNPKTEIHYLPYTKSISFAKKVWEGESAIWGVAFVGTSPKIDAT
                     HTGFLILENGKKPVLRHASQLKKKVIDQNFDAYLKSRKGKLPGVVFFNFLEL"
     gene            complement(20145..20582)
                     /locus_tag="PLT31_00085"
     CDS             complement(20145..20582)
                     /locus_tag="PLT31_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93561.1"
                     /translation="MRQKQITSIIQKYMPMLNSDSLVFQELLKCFSENALIQPDTNDL
                     ISFLSPRRLFNVIKIKSSSAEGCSKELLQKHPLFSEAAGMIRYYNTPNDVNWADIERA
                     ETTIGKTLAMDIYDWKPDMFTVFNSEKNTEYELVMIIAFSSEF"
     gene            complement(20686..22350)
                     /gene="pgi"
                     /locus_tag="PLT31_00090"
     CDS             complement(20686..22350)
                     /gene="pgi"
                     /locus_tag="PLT31_00090"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HPW93562.1"
                     /translation="MSHLTESNEWKALEAHFQDAKTWHMRNLFAKDPARAKKFSAEAC
                     GLFLDYSKNIITEETLNKLFDLLKSTGFEAKRDQFFKGEKINHTEKRAVLHTALRYQG
                     KNSIMVDGKDVMPEVKAVLKHMEEFTNLVRSGKWKGHTGKSIKYVVNIGIGGSDLGPV
                     MVTEALRPYASEGSPEVLFVSNVDGTHIAETLKKVCLEETLFIVASKTFTTQETMTNA
                     ITAKEAVLKAFNGDEASIAKHFVALSTNAKAVSDFGIDTANMFGFWDWVGGRYSLWSA
                     IGLPIALRIGFDNYNALHQGAYEMDVHFKTAPMDQNLPVILAVLGLWYNNFFGAESYA
                     MLPYDQYLHRLAAYFQQADMESNGKTVDRECRRVDYQTGPILWGEPGTNGQHAFYQLI
                     HQGTKLIPCDFIAPANSHNKIGDHHRKLLSNFFAQTEALMNGKTLDVAAEELRAAGKS
                     AEEVAFLAPHKVFEGNKPTNSLMMDKVSPKTLGALIAMYEHKIFVQGVLWNVNSYDQW
                     GVELGKQLAGKILPELEKGEQNPNIQLAHDSSTNSLISWYLKHREV"
     gene            complement(22493..23062)
                     /locus_tag="PLT31_00095"
     CDS             complement(22493..23062)
                     /locus_tag="PLT31_00095"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HPW93563.1"
                     /translation="MNKSIKLLIASVIILACSVGFFIGATVFQGSFTLKTTDSKSIER
                     DDSNRRWERNRSDRKKNRERRLHSSLDSILQLTPEQKVKLDSHRVVNDSLRSISFENF
                     KQAETELQDALAAQPIDENAVEQAKKKLLQLNEKRLDQRIKDIRFFSATLTPEQHRSF
                     KSLHRKKGFRPRNRDLPPPPPTMNSIEQE"
     gene            complement(23076..23393)
                     /locus_tag="PLT31_00100"
     CDS             complement(23076..23393)
                     /locus_tag="PLT31_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93564.1"
                     /translation="MPDSLNLNSIPKIMPKEDSWNKVLLRINAERKAKKIIPFRLYSI
                     ASVAASLLFVIGGIFLGISSNQGNLDSSFESNENVETLSWYASLGEGASMEEFSSILD
                     EYK"
     gene            complement(23386..23928)
                     /locus_tag="PLT31_00105"
     CDS             complement(23386..23928)
                     /locus_tag="PLT31_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPW93565.1"
                     /translation="MNENDLKRKLLKADADTLSVLWKAHSVSILNLAFRILQDRDAAE
                     DVLMDLFVDLPKTIQSFRGEASFSTWFYRMTVNACLMRIRKSKRHSELEELNSLSISE
                     NIIGRPDEVDVFDAKLLEYGLSSLPAETRSMLWLKDAEGLELKELSEIFNLPEGTLKS
                     KLSRARQHVREILKEEKVYA"
     gene            24264..24806
                     /locus_tag="PLT31_00110"
     CDS             24264..24806
                     /locus_tag="PLT31_00110"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HPW93566.1"
                     /translation="MWAMDLTGITDSAQKTRNILGIIDHGTRKCLCLKALGDKASITL
                     LKILIQAIDAYGKPKAIRTDNEAVFTSRLFNFGLWLLGIRHQRTKLHCPWQNGRIERF
                     FGTFKLVADRVLFEAQNFQFSLNEFAFWYNHIRPHRHLNGRTPDEAWSGIDPYLQAPK
                     SCRKFSAWDGMLTGFQMEYG"
     gene            25051..27834
                     /gene="metH"
                     /locus_tag="PLT31_00115"
     CDS             25051..27834
                     /gene="metH"
                     /locus_tag="PLT31_00115"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HPW93567.1"
                     /translation="MSLNLSESYKKLQKECQERILVLDGAFGTMIQRLNLTEADFRSS
                     LFKDHPVDLKGNNDVLCLTAPHHVQNIHRLYLEAGADIIETNTFGANRLGQLEYHLAD
                     RVYDIARAGAVAAKVAVEEFNQNRVAQGLSSVVKFVAGSIGPTSKTASMSPDVSDPAF
                     RAISFEQLAADYGEQVRGLLDGGVDILLVETIFDTLNCKAACYAIQKECSRRGELFPI
                     MISGTITDASGRLLSGQTSRAFWHSLSSYPIFSIGFNCALGAKEMFAHLREIKNAPLF
                     VSVYPNAGLPNQFGGYDETAEMMASVIKSYAEENLVNIVGGCCGTTPDTIRAFSKALA
                     GFKPRVVPSASKSLLLSGLEPLTIDANSRFVNIGERMNIAGSLKFANLIREGRFSEAV
                     SIGVQQAENGAQVIDVNLDDGMIDSKSTMIHFLNLLMSEPLVARCPIMIDSSKWDVLL
                     AGMRCVQGKGIVNSISLKEGETAFLEKAEEIRRHGFAVVCMAFDENGQANTYERRTSI
                     CTRMYRLLVDKISFPPEDIIFDPNILTIGTGMEEHASYGKDYIDTCAYIKANLPHAHI
                     VGGVSNLSFAFKGNNAIRESIHACFLYHAAKAGMDFGIVNAGVLPVYEDIPEQERSLI
                     EDLIFNRSPEATDKLLAYAETIKGRKGNAPDSAEFLKWRSLSVEERIRHAMVKGIDQF
                     VEEDVEELRVRTANPVDIIEGPLMDGMNKVGELFSEGKLFLPQVVKSARVMKRAVAVL
                     LPYIEANAKVGSSSKAGKVVIATVKGDVHDIGKNIVSVVLGCNNYEVIDLGVMVPCEK
                     IVEAVKEHKPDLVGLSGLITPSLDEMVNVAKAFKRENLKTPILIGGAAASSIHTAVKI
                     APESDAPVIYTSDAGKCVTIAEQWTNLRKRDHFLKEHLSHQEELRSLHAKQQSQVQTI
                     SIEEARSRGESLF"
     gene            complement(27845..28609)
                     /locus_tag="PLT31_00120"
     CDS             complement(27845..28609)
                     /locus_tag="PLT31_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93568.1"
                     /translation="MPNNKRSWPLTVYIVLRLLVIGIMVIQFFNQNYTDVFLCLLTLI
                     LFQIPTFIDRKFKIEFPDTLQIVVLLFIFSAEILGELREFYLQYPIWDTLLHTTNGFL
                     MAAIGFSFIDVFNRSEKISTKLTPISLTLIAFCFSMTIGVLWEFFEFAMDYFFKMDMQ
                     KDWMIHEITSVTFHPEGRNTPVTLPIESVVVNGKAWEGYLDIGLFDTMKDLIVNFIGA
                     IVFCLFGFAYIKNRGKDSLAKKFIPIVRSQKKNAQN"
     gene            28685..29905
                     /locus_tag="PLT31_00125"
     CDS             28685..29905
                     /locus_tag="PLT31_00125"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HPW93569.1"
                     /translation="METMDLNTFVNQRWFLHKNNSIQKGNVVHQFDLSDEYAFCVFAF
                     YLDSGKRVLYGYPMVKNRVDLDLMNYPEHHALFMNALFQKKIWSGGNATLKIHYYDQE
                     AFEKPFENSIKQNLEQSNSSILFQGGRFLKWFRFLNAGEHPEVEMGVFLQLHHSKDTA
                     KILAHISYDVNGEYYVVGVIESALPFEKIAWDYFGENPSEEKAYLLGTQLASLHQTLL
                     AMEGEFKNRENNRLEVYWNAFQANLKQSESQLQGRSLEAAKRILSKWSLIEEYVKSLA
                     QVSFLAPVSRIHGDLHLGQILYKKDQFYFIDFEGEPIIPLNERREKRSVLFDVACMIR
                     SFRYAKAFFKRSSSAALELAFLKGYGVESSDELRFFLFQKTLYEASYELNSRPDWFYI
                     PAEDLLNHVLTNQL"
     gene            complement(29975..30048)
                     /locus_tag="PLT31_00130"
     tRNA            complement(29975..30048)
                     /locus_tag="PLT31_00130"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30012..30014),aa:Ile,seq:cat)
     gene            complement(30049..31452)
                     /locus_tag="PLT31_00135"
     CDS             complement(30049..31452)
                     /locus_tag="PLT31_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HPW93570.1"
                     /translation="MNFLVVSPEAGRWKTPSPLVQAVNHMTDAYARVGIEVVTCSPFY
                     PHQLGDFSSFTCIFEGIEELHHLPFQIWKGKDARHTYIFNSELFNRPAIYGENGYAYA
                     DNHIRFSFLASAALSYCKTIGYKPSAVLGHEWGAALTGALMQTTFSDFYSDLPFFFTV
                     HNITYDCHVYDTEIERIGLNRKDYNMDGYEFWGKVSLLKVGLCYARTVLFPSPGYRDS
                     MLKSNLPGGLSGFLNHNEKKLKGIQFGVSYALWDFNLKTGIPICEAKKMARKNFETQL
                     NASFQDKLVIYTHLDNESGKTSETLATIISDITKLNAFLVVGILKENPDWNYYQSLVQ
                     EYPQNIAILDIFESKSALRVSLAGSDLLFASNLQEPSASLVLKALASGTIPLSGKDVG
                     VANLLHDFEGDATLADALLVDDSAAPHQMLRRLREAESLYCNEKSVWEKIVSNAYLFR
                     YEWDRTISQYLLTLGEH"
     gene            31581..32630
                     /locus_tag="PLT31_00140"
     CDS             31581..32630
                     /locus_tag="PLT31_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93571.1"
                     /translation="MKKMLFLVFMGALVALTGCKGTNEKRGDEHLEAGRFRNAINSYL
                     EAKKKGKISDEFYDNFALALIRAADAEAKKDLNSQLLSGYFEQAQKALDSAKKDEVTQ
                     EFVTALANLGKLQAAQETDYGTVLNAFAKLDTAMVMAKRKGVGEGAVKTIRAEAENAY
                     VKVALPNALSEEDPVVREYFLLQIDVVAPENAELKEALNKSRINTRGYFLIFGENIGE
                     RPSNRVDKWGYVMAFPSIKISSTSLTGELQVWASTGNNTEFVVDKIKLVSTDGKEVFA
                     KQTGSGWCEAEVVVGKKGDEKVEKKKASFKGKGKLLNEFQCSANIAFTFDSSFIPDYV
                     EYKDEMGIGRKYLGR"
     gene            32755..33588
                     /locus_tag="PLT31_00145"
     CDS             32755..33588
                     /locus_tag="PLT31_00145"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HPW93572.1"
                     /translation="MFYLGCHLSSSDGFYAMGETALSIGANTFQFFTRNPRGGAAKAF
                     DPEDAERLVQFLDKHSFGPILAHAPYTMNPCALDSGLREFAETMMKDDLFRMNHFKKS
                     MYNFHPGSHVKQGVSVGIDFIASMLNRVLSPSQNTLVLLETMSGKGTEVGSRFEELQS
                     IIEKVECSEKLGVCLDTCHVYDAGYDIVNHLDDVLDEFDKIIGLSKLKAIHLNDSKNP
                     FQSHKDRHETIANGSIGLEGILRIINHPKLKSLPFFLETPNELPGYASEIKMLRERYE
                     G"
     gene            33578..34258
                     /locus_tag="PLT31_00150"
     CDS             33578..34258
                     /locus_tag="PLT31_00150"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HPW93573.1"
                     /translation="MKAKELRLFLKENVDLKYQKFSASLLPGVTDLWGVRLPVLRRIA
                     KKIAKEGNVDLLKSSLKKPCFEERMVLGMAIGYLPWDDIFLEKYVEPYIPLIDNWSIC
                     DSFCVGLKQVAKNQEETWHFLQKYFKSDLEYEVRFAVVLSLFYFLTDSYFSLVLDQLA
                     LIHQEDYYAQMAIAWAIAKAYAVSPEVTKKWIKKTKIKQEILKKALQKIRDSLVTKSS
                     ASAFECLI"
     gene            complement(34227..35219)
                     /gene="opgC"
                     /locus_tag="PLT31_00155"
     CDS             complement(34227..35219)
                     /gene="opgC"
                     /locus_tag="PLT31_00155"
                     /inference="COORDINATES: protein motif:HMM:NF021633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HPW93574.1"
                     /translation="MTLDHFGKPISSYLFQCFGFFSAAEGFFFLSGFVGMFAAISKRE
                     KDPSASWMRARAFRIWKYHIISLTCLVCSSILFLPALVPYFRGILQHPHTGSLLSALL
                     IHTPTWLDVLPLYVILMLIGSFFFPRFARGSVYKIWFLSFLVWILAQFGIRENILALF
                     PKWVNHGFFDILGWQYVYFSGAAIAAWWKHHQKAKTTPHRFVQNISYVAIFITIFLFL
                     WSHQWISLPLPSEFWISREHLGPLRYLNFIAFALSICFIVRTRPSLLDFQFSATLGKH
                     SLEVYTAHTILIYIWLIVPKYVHLHMPWNIIFPLFACFLLWLLAFILDIQKQKR"
     gene            complement(35276..36382)
                     /locus_tag="PLT31_00160"
     CDS             complement(35276..36382)
                     /locus_tag="PLT31_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93575.1"
                     /translation="MRKLILPIALMTAVFTQAADIEVNGNINADYATYWDKDFSPTNA
                     ANQDIDLSLTGLLNESFSATVKTNIQGTYMDSLGALKASEVRHTQSRATAMGDTANRF
                     TNIKFDGVFFRWQINPNVTTVFGDLSYSAGSFNYYFWRDSSRYNVISKEQRLRGIGVE
                     LEGGKVYIGASENNSSSILAFGSYPVELLSRTEERLVLTPSIEWAFGSDIGRSYTYAL
                     GLEAVYAKSKGEMNYGITATWGTHPYKGEGVHSFLVEPSFNYDFFNIGATFYQALLAE
                     RDSSVQAQIFTEDQTLFFIEPSIDLHQKVSLGIAYEYHDPSNKIKSDHRQFIGPNLYF
                     YPTINSEIVFWGGYNVLPKKANNFSMGISGHTNF"
BASE COUNT        11001 a         6374 c         7648 g        11530 t
ORIGIN      
        1 ccagtagctg gtactgtcac agcaattcat attaaagagc aagattctgt ttcagaaggt
       61 caagttcttt tgaacattgg ctaaggataa cgttatgttt gaaatttttt cctccgttag
      121 cgaaatgctt gtcaatgata ctggttttca gtatatcacg ggaagcatgg ttgtaatgtg
      181 ggtggtttca tttgttttga tgtatttagc gatagtcaaa aaatttgagc ccctcttgtt
      241 actaccaatt gcttttggtg cacttgctgt taatttacca accaaattat tttatgatgg
      301 tggttggact attgaaggca tgtttacacc gacagctggt ctctactatt acattagcca
      361 gggcattcac ttagaattat tccctcctct catcttttta ggtgttggag ctatgactga
      421 ttttggccct cttattgcta atccaagaac attgctttta ggcggtggtg ctcaatttgg
      481 tgtttttgca acgatgttct gtgcggttgc ttttggtgga tttactcttg gcgaagctgc
      541 ttctatcggt attatcggtg gcgcagatgg accaacatct atctttactg caaataaatt
      601 agcaaaacat ttaattggcc ctattgctgt agcggcttat acttacatgg ctttagtgcc
      661 attaattcag cctcctatta tgcgcttaat gaccactgca aaagaacgta ccatccgtat
      721 gaagagctta cgtaaagtca gcaaattaga aaggattgtt tttaatatca tggtgatgat
      781 tgtctgtgtg ctagtggttc cagatgcttc tgccttgatc attatgttaa tgcttggtaa
      841 cttaatgaaa gaaagtggag ttgtggaacg tttagtaaaa acagctcaaa atgaattgat
      901 gaatatagtc accatctttt tagggacttc tgtaggttta accatggcta gcgacatctt
      961 cttacgccca caaacattgg cgattattgg tatgggtgtg gttgcctttg gtttctctac
     1021 aatgggtggc ttacttctag ctaagattat gaacgtacta agtcctaaaa atccagtgaa
     1081 cccattaatt ggttctgctg gagtgtctgc agtgccgatg gctgctcgcg tttctcaagt
     1141 ggaaggcgcc aagtacgacc cacaaaactt ccttctaatg catgccatgg gaccaaacgt
     1201 cgctggtgtg attggaactg cagttgcggc aggttatatg atttctcgtc tagcttgatt
     1261 tattttaatt atcaaaaaaa gcgcttcttt tttaagaagc gttttttgtt ttaaatgctc
     1321 gttcagtcta atcgctgtga ttggctttgc tatttaggcc agtagtcgtt tggcttttga
     1381 ttcagataaa taagtttagc ccaatcttcg ctttgtactt tgttttggaa gaataattcg
     1441 tcgagcattc ctttgaaaac acgatcgacg tttccatctg ccattttacg ggctccaatt
     1501 acaaaggaac cacctgcgat tctttcttca tcggtctgat tgtaacctgt ggtaacattt
     1561 tcaagttttc catttttata aatggcggtg gaatcaccag atttaataac tgtgaaatgc
     1621 acccattgct taaagtcggt ttcgggggtc gccacaatgc ttgattcata ccattcatct
     1681 gccttggatt cgtcaaattc cttgaacatc cagcaggcat ccgattgccc tttggcttgg
     1741 tacttgaagt ggtattctgc ttcagattta ccccaaagga attttgctgt agaggtgtct
     1801 tcggagaaag cccagaaaga tactgtaaag ctcgaggtgt ctacaagcga gaatcctgaa
     1861 taatcagcag attctttgat ttcaatgtaa gaagttttgc cattaaaata gaacgctcct
     1921 ccaatgattc cttcgcttgc ggtaatatcg tttacatatc cctgaaagtc gttgtcgcca
     1981 gcggcataga tggtcgattc tggaaaagcg tcttcgttga agtcccatgc agcaataaaa
     2041 ttatcagaaa gtgagaatgg ttttacggta tttactgcag aaatgtttga gtcttccact
     2101 tcttcccaac tcaatgtgat tttttgcatt tctctttgtg ggtaaagtgt agtcaatcga
     2161 atccaaagtc ctgagggtat agaatctttg tcgtaaacga ctttatatga ggtctcatca
     2221 gaagctggtt cttgatagat tttgagggtg ttaagatgct taatgattga ttctcgattt
     2281 aaggaatcaa tgtcatttaa gcgaatgaat agaggaaatc cgttgagagt gtcttgaagg
     2341 tcaattccat tagctgctgt gttcagtgaa atgtgatgtg tgcggatcgt gttattactg
     2401 ttgtaaattt cttgactttc ttctttgaca aagattgagt ttagtacaat catatcagaa
     2461 gaaatggcga tttgtatgga gtcgtagtag gcacttggta tgtttgtaaa agtggcgtat
     2521 gtgccgattt catcttcaaa aatttttgct gcggagttgt acgaggaacc gactatagaa
     2581 agatttgcaa tggaatcctt tatgttagaa agatagattt taaggctacc acttgtgggg
     2641 attgttaaat tcactttcca agtagaatct ttttgaatgt ggatgcctga ttcacggaga
     2701 gcatatacca ctccttgtga gtcgttaaaa aaggcttcga aattgtatag gccgttagga
     2761 agactatcga atacaaagtt ccccatggag tcggcgatgg ttgagtaata ggaggaagta
     2821 tcttctttga ctgaattata gtcgatagga atacagtaaa cacgtgcgaa agaagctcga
     2881 ttttgtttgc cattattcaa tgctagtgta ccgagcattt ctggagaacc gacttcggaa
     2941 gtgttccctc ctgctgagat cttgttgtca gaacaagata caacgaagct tagtgcaaat
     3001 aagattactt gcaatatgat caatgaagaa attccctttt tgattaatgg agtatttctc
     3061 atcattgaat cctcatttgg agagagggaa aatttgtatg ttcatttgga atacgcgtcc
     3121 gcctttttca tcttcattag caatcataga tatttttttg cggaaatcat tgatacactg
     3181 gacgattttc ttgtaagatt cctcagaaat gcctagtgta acactactaa tgttccgttc
     3241 tgatgatgaa aagttggtaa tggagtcaga agcaagcgaa agacattcct tatggtagtt
     3301 aataaatgcc tgttggatga ctttgtcgcc tgtcgtgatg gctgcattat tcagaaccca
     3361 attgccattg cggtcttttt tgataaaacc tacacgttcc agaagattta cagaattacg
     3421 tgtctgttct gcagaaatag gtggagtgag ttttttgcca agagcggcgt aattcccgtc
     3481 aaaaccaaac ataccaatga tcgaacggat ggcggaataa taccaatgcg agtagatttc
     3541 gtattgataa gctcttgtga gttgtgtttg ttgtataaac ttgtacgtgg ctgcaatgga
     3601 gtttacacgt tgttgatact gttgccgttc ttcttctgtt gttgcttggc ataataaaac
     3661 gagtgcttca aaatagcttg actcatcttc attcatttgg aggccattca taattttcag
     3721 aatactggat ggtgtgagat tcttttcgcc attcatgatc cgaagaatga aatcctttga
     3781 tgaaaatcca agtttatttg aaaaaagacg aagtgaatat ctactgttta acgccttact
     3841 ttcttcaaag tagttgcgca gatattctcg ataattatcg aaatcaaata cggactttcg
     3901 gccattctgc tcatgttgct ttatggtttt catagaatta aaatacattt ttttcttttt
     3961 ttcacgaata taagcgtttt gtgctttcta aaacgttgtc tatagaatac acttttgtct
     4021 tggatcactc ttttatttga ttatttaggt attttgcctt tagattagaa gtcgtgtaaa
     4081 cagggtgttg gataaaaaca aacagggtgt aagatgaaaa aaaatatcta taaagttttt
     4141 ttggcggtag cgtcatttgc tattatggca acagcggccg ttgatcagtg caaaccaatt
     4201 ggatgggcaa ctacgagcgg acgaacgagt accgcatata atgtgactgg tggcggaaac
     4261 gcaacaccta ttacggtcac taattttgct gatttgcaaa aatacatgaa tgatgatacg
     4321 ccgagagttc tctatgttaa agggcctatc ggtagcggct ggaaaggagt cacaggcgac
     4381 cgtcttgaaa tcaagtattc caataagacg attatcggct caaatccagg aacagagctc
     4441 aaggctacta tccatatctc aaacaacgca cagaatatca ttatccgtaa cattgtgatc
     4501 aatgggcctg gttccaatca agaccaagcg tgggacaatc tcaacattga gggtggtgct
     4561 aaaaatatct ggattgacca ctgcgaattt tgggatggac aagatggaaa cgctgatgtg
     4621 gttaagggtg ctgataatgt gacttttact tggtgcattt ttggttacaa gataaagagt
     4681 agtcataatc tttcgaacct gattgcaagt tctgacaatg aacctgtcag cgaaggaaaa
     4741 ttgaatgtga cttatgcgtt caattggtgg aaggctgcaa cacaacgtaa accacgttgt
     4801 cgttatggca atatccatgt gatgaataac ctatttactg gagataagaa tattcaaagt
     4861 ggttctatca tgggcatttc tgcaggtgta aagtgctatg ttcgtgcaga aaacaaccat
     4921 tttattgatg aagctactcc tattcacaaa cgcgatggcg gtgtcacaga atccattgga
     4981 gataagttta ctaactgcac aggtagcaca gcgagccttg gggcgtcttt tacacctcct
     5041 tacgaatata aatcttacat gattagtgct gaagaagtgg aagccgttgt gaaggctaat
     5101 gcaggagcaa cactcactag ccctacgcag tgtaatagta atgttactcc agtaagctca
     5161 agcagtgttg tagctagttc aagtagtatt atagcaagct caagcagcat tgttgctagt
     5221 tcaagcagtg ttatagctag ttcaagtagc attgttgcat ctagtagttc cgtagaacgt
     5281 ccagaaggct ttatacagat tgaagatgga caaatcactg gcggtgtttt ggaaactaaa
     5341 aatagtggtt tcaaaggacc aggatacgtg aatttcgata tgggtggctc cgtggtcgtt
     5401 tcagtcacgg tggaggcagc gggtcaatac gaattggata tttattatgc aaatggttcc
     5461 actggaaatc gtagtcttga aatatcagca ggcaactcta tggcattgat tgaattcgga
     5521 aatacttctt cttggaccga ttggaaaacc gaaaagtgca agttggatct ttctgctggc
     5581 gaaaactcta tcaagttcgc cacggtcgct gaaaacgatg gtccgaatat agaccagttc
     5641 agtatggaat tactaaagac tactaatatt tccgattcgg acaataatgg aagcctcttc
     5701 actgctaatt cacaacagtt ccatgtgatt ttgaatggtc gaaatatgca gttccagaat
     5761 aaggttgtat ctgcgcaagt attctctatt atgggtttgc caattaagaa tgttcagaat
     5821 acaaatcaga tgaatcttac agatgttcca tcaggaatct atctgctcaa aatctctgtt
     5881 gattcaaaga cgcattttca aaaaataaaa ctggattaaa ataataatcc agttgggcta
     5941 actttgaatt aaagtgtgag attttgggaa tttaaaatga aaaaaataaa ccttcgtcta
     6001 ttgcagaaag tagtttttct taccattttt ttgatatctg ctttgtttgc agaaacaaag
     6061 accattgctt tctttacgcc ttggtctaac actaatgcgg ttctttttat gaatggtgat
     6121 tccgttacta ttatgacacc gctcaaaaat tattgtggat ggtatagggc tgaagtcgaa
     6181 gtccctgcaa gcggttttaa cgttcatttt aagcaaacag tggggctcaa ttatgtgggt
     6241 gctgagggta tgacaaccat tccacctacc atagcttctg aaatttctct tgattctgta
     6301 gcattgcttt cagacacgat ttgggtgcaa ggctataaaa atgatattcc tgctctattc
     6361 tctatttatc ctggcgttct tggcgattgc ccgcttaaga aattccctgt gactttattt
     6421 gactggcttc atggcgacaa tggtgatgga gacgaagaag gaaagaatgg agaccctatt
     6481 aatggagtta gtgcagattt tggatcgggc ggttgcagag gagcaagtca tagtactggc
     6541 atcatggtgg gtatggtcga aaaggaatta ggtgaaaatg gtttacctgt tcctgctaat
     6601 ccgttcccag aagattgcaa aatctcaaca catctttcaa gttggttttt acccgaagtc
     6661 attgcgcagg atgctgaggg aaacaattac accaatatga cgtgtcgaga cctctatatt
     6721 tctatggatg atgaaggttt ttggcttgcc gaagtttcga aagatcaaat ttctgaggga
     6781 aacgaaaagc ataaagatgg aatgttccca ttggatgact ttcagtactt agatgaggcg
     6841 atgacagtat taaatcccta ttacgaccag ttcaaatcta cggatggtag cggcgataaa
     6901 cacaatttcg gctttaccat gaaaatccaa gcgacatttg agtatgtgcc aggccagtat
     6961 ttcgatttct acggagatga tgatgtatgg gtattcatca accgcaaact agtcgtagat
     7021 attggtggac aacatcccca aaaggctggc gctgtcaacc tcgattcact aggccttgtt
     7081 cctggagaca aatatgattt ccatattttc tatgctgaac gacatacggg atcttcaaat
     7141 ttccgaatga gaacctcaat tgatttgcaa gtagatgcat cactcttttt gactaataaa
     7201 agaaactcta attccgtgga ctacaaaata tggcaagtca ataagaaagg cacttttagt
     7261 tgtggtaacg atattaatac tacggaactt gatacgactt ctggagctgc tattttcaag
     7321 cttactggtg gcaatcttgt cggccctgaa attctagctc agggaacccg ttacgaagga
     7381 attcatatta caagtgattc cactttcagc attgattctg ctgcaattat cgcaagttat
     7441 gcgcttgctc cagggcatta cttcttggag gtcactttga agtctgatcc gagtcagatg
     7501 acaaaaattg aaataacgat cccttcgtat gcgattccga gcgttgcatt tgcaaagatg
     7561 gattggacta ttcttggtaa agatgtttct ggtgatacgg ttcagattgg taactggacg
     7621 aacgcctttt accaagtgaa tattactttt ttagaagaat gggctaaagt caataactac
     7681 aatcgtaaaa taaacttgtc tttttctgac ccaaatattg acatttttga tactattgat
     7741 ggtcaaaaaa ttagttctgt aagccttgac gataatggac gagcgacttt ctatgtatat
     7801 gcgaatgcac ctgttgcggg agcgagctta attgctaaag gagctgccgc aggagtatcg
     7861 gtatggagag atttggattt tgtagagtcg ccgattccaa aggttttaaa agcagtcatt
     7921 aaggatcgta atggagatgg tcgtgccgac agcttatttg tgcgttttga tcgcagtctt
     7981 ttagaaaaga gttctcttga ttccattcaa attacttttg gagaatcctt taaggtcaca
     8041 tctcaattca gcttggtgaa cgaaatggat gttgttgctg ttgccgaaaa agtgagccct
     8101 gaaaattgta ctggtgatgt ttgcggtttc ggaagtcgcc tatttacagg aagtaattcg
     8161 gatgtttact ttggatctct taataactgg tttagctata aggatagcag taaagtcact
     8221 aatttttatc aagaaaataa tcccattgaa gatggtgttg gtcctataat cctaagtgct
     8281 gctagttcta ggattaatga cgccaataag tccatttcac tcactttcag tgaagctatt
     8341 tctgaggaat cccgaaataa taattacatg gaaatgtttg aattcatttg catgcgatca
     8401 ggtgtaaatg aaataccaga atatcctgcc atgcaatcaa gctctcagaa caccatggtg
     8461 tttgtctata gttctacgga tttagatgtt gtagttccga cggctggtga tcagattcgt
     8521 tttaatcctg ataatgaaac aaaggacctt gttggcaatg tgcctcacaa gaataaccct
     8581 tgggttccga ttactggaga acaagaattt tcaaatgaga gccccaaagt gattactatt
     8641 ggtgaggatc cgtacaatat tattaagaca gatattatta cgcagccaat gattattacc
     8701 aatttaacac agaacgcgca agaaataggg gattctcttg gtgtgcaagg aaacttgatt
     8761 gattacgact tttcaaaaat cgtaattgag aaaacaaaaa aagaagctca aacactagaa
     8821 tcttttattg aatccagact tgaaaatatt acaagttatg ataccttgat cacaagcatt
     8881 agcgaacagg aggctctctc acaagtgttt gcagacatcc gtacaggaga acttaattct
     8941 atgtatggtt ttagcgaaga gacgatcgct gccgttattg caggaacgat tactgaagaa
     9001 aattttaatt cgagtatttc ggaaaatgaa ttgttggtta ttacaaaact gatgcaagat
     9061 aatattgatg ctacccgtga tacagtgatt tcactaagtg cagcgtctca ggtaacacag
     9121 tcagatctat tcaatgcaat gcgcaacggt agcctagata aagaactgaa aaaagcaggt
     9181 gtgagccaga cgcttatcga tgctatcaaa aacgatgaag tcacagaatt taatctagaa
     9241 gaattcagta gaggttcaaa gttagtctat gcagaggatg atgttcaatt tttctaccgc
     9301 actcattatt acagccatct cggcgaatat gtggggggta cgtcaaattc aataaaatgc
     9361 tcagacaata atgtgtttgg cgaagaagga tgtattcaaa ataaaggtaa gatgtttcta
     9421 gcttggaata tgcgttccac tcaaggtcgt ttggtggcca caggtgtata tatttcactt
     9481 cttgaaataa aaattatcgt gaatggcaag acttctttac atcagattag taaaaaacgg
     9541 tggggcgtaa aacgcagttt gattaacgaa ctacaactcg aattttaacg ttttctaccg
     9601 aaaccaaaga acactattct acaaaaacaa taaaaacaac gcatacaaca aaaaagtgtt
     9661 gatgataacc atcattcact tttagaagat tttttatttt ctctaatatt ttttgttgaa
     9721 tcttgacaat atgtcaaata tgatatatat tataatgtat gagtccgaaa cataaaccgt
     9781 tggcttggct gagtgatttg ccaaagtcac caccgatgtc aatgaatgca agaattgaga
     9841 ttggctatct tttgcgactg ttgcaatctg gaataaacgt gtctatgccg cacagccgtc
     9901 caatgccatc gattggtaaa aggtgtcatg aattgcgagt gaatgatgaa aagtgtactt
     9961 ggcgactttt ttataggata gatgaagacg ctattgttgt gatgaattgg gagtttaaga
    10021 aaactgaggc gacttctaaa aagatgattg aactttgtca aaaaagactg agtgactacg
    10081 actcaattta aggagacgat gatatggaaa aaataaagaa aaagaaatta gaggccaaag
    10141 gttggactgt cggtgatatt gatggttttt tgggtcttga tgaagctgag atgacgattg
    10201 tagaaatgaa agtcgctttg gcgaaagctt tggttgaaaa aagaaaaagt tcaaatgtca
    10261 cacaagtgaa tatggccaaa attattggtt caagtcaatc gcgagttgcc aaaatagaaa
    10321 aggcggattc gagtgtttca cttgaattga tgataaagtc ttttctttct ttgggagcaa
    10381 cgaagaaaga tattgtaaaa gtttttgaca aataaaaaag tggatcatga cgcttcatcc
    10441 attctttttt taaagcactt cttcgcaaac catcattttt ggtgctgata atttcaaatt
    10501 taatctcgcc aatatctcat tcctcaaaat gaacccacac tctgaccgaa gcacttagtc
    10561 tcacaattat caaagagacc ataaaaaaaa gacctgtaaa acaggtcttt tttagggtga
    10621 gagatcggac tcgaaccgac gacaaccaga atcacaatct gggactctaa ccaactgagc
    10681 tacactcacc acgaatgtaa ggtcaaatat aataaagggt tcaagagttt caaagtgtta
    10741 gagattaatc gtctctattc tttttgctca taatgcgaat aaaattcgct cttttaaagt
    10801 cgtttctgtg ttccatacag aatcttgggt acacaaattg cttagggaat accttaatag
    10861 taaaatcaaa gtcgcaacca tctaagcagc atttgaaagg taagtccatg acttctgtat
    10921 agttgtgcct aaagatgatg tttttagact caatattttc gatatctttt ttctgttttt
    10981 gccgttgttt tatatcacgg tgatcatcac agtattttgc aatgggatgt ccccaaaatt
    11041 ctttgccgca acccggttct tgacaaactt ttagtttaac acgtttcttt ttcttgtact
    11101 ttggtaattg cattaaatac tccaagtggc gattgaagat gaaatttagc aaaaaaagaa
    11161 aaaaaaacaa attttcatct caagtaatgg atcttttagg cttataaatc gtttaaatgg
    11221 tatgcatact attcttttta ctctgattat tctgttgtgt tcggctttga tgtcttgtga
    11281 agaaggctct tttttagaag aaaagccatt aaaagtccac tttttagatg ttggacaagg
    11341 tctttctgtt ctttttgaat atgaagggcg ttttgcttta tgggatacgg ggccagattc
    11401 tattggtttt gtggatacat taaaacatta tggagtggat tctctagatt gggtcgttct
    11461 tagtcattgg catcgagatc atgtgggggg atttcttgaa ttcccttcgg cgatcaaaaa
    11521 tcgtgaatta tttgtaaaaa aagttttttt tacaaatgac acggcctatg ggtttgtttc
    11581 agatagtgtt ttttcaatat tagatgaatt tggcttaaat aatgatacta tttacagggg
    11641 agatagcctt attttcgcct ctgggtggcg ttttcagata ttatggcctt caaaaggcat
    11701 caacttaggt ggaaatgctt ctagcggtgt tctttttatt cgtgattctt taaataaaat
    11761 gcttttaatg agtgatttag agtctagaga ggaagaatct ttattgtctt tatacccatt
    11821 tttaaaaacg gacctactgc aagtagggca tcacggctct ttgaattcaa gttcaataaa
    11881 atttctttcg caaattttgc cttctgaggc cataattagc gtaggagagg gaaattctta
    11941 ccatcatccc gcgcagggaa ctctctcaaa acttaattat gttgttggag atagttcgtt
    12001 tgttcatcga acggatttgg agggaacatt aatttggttg tggcataaaa atataggctt
    12061 ttggatgcca taatttttct atcttgtctc gccagatgat acatactaca catattcccg
    12121 taatgggcac aagttataca attgatactc ctattcgagt agctcctttt ggcatagatt
    12181 ccactatttc tttagtagat aattatttat tagaagatct tagagcatat tatagccgtt
    12241 tatataatct tccatacgaa cctattttaa gaaatgaaga agatggttgc gctaagagag
    12301 ttcaagccta tttagatatg gttcatacga ttgtctgcag gcgttttgaa gaaatacgaa
    12361 atcttccgtt tgaaaaaggc aatgaaaaag aaaagtattt tacgatgttg ccttctgctc
    12421 atgctcttag aaaaggtttt gaatccgttt taaaaatgcc tttaggtgcg gagcgttctg
    12481 ctcttgaagc ctctttgaat tctcaaatgt atatgggctc tatcgatgtg aatttgatgg
    12541 tcaagcttga ttataaaaat gtggctaaag atgcactacg tggtttcgct cattccaaga
    12601 ttacgaatgg tgctgttgtt tttagtgctg gcgttaattt agggcttttt aatgatttgg
    12661 tgaaatatcc tgatttttat cgcacgaaag atggtattaa gaaacgtatt attttaaaaa
    12721 tttcagatta ccgttcggcc ttaactcaag gcaaattttt agctaaaaaa gggctagaag
    12781 tatctgagtt tcgtgtagaa tctggcttga attgtggtgg tcatgctttt gctagtcaag
    12841 ggatgctttt gcctttgatt ttagaagaac ttaaagaaaa aagagaatct ctttctaaaa
    12901 ctttttatcc attaatacaa aagtattacg atgctcaagg gatggattct tctgttctcg
    12961 atccagatcg cgatgcgcgt tttactgtcc agggcggtat tggtaatagt ggcgaaaatc
    13021 gacggatgct ggagtattat aaagtcgatg gcactggctg gggttcggct tttttaatgg
    13081 ttccagaggc ctgcttacta gatgattata cgcttgaaat tttaagagat gcaaaaagag
    13141 aagaccttta cttaagtgac gcttctccta ttggtgttcc attcaataat acaactcgat
    13201 ccacagcgaa tgtacagcgc ataaaaaata ttgaagcggg ctcttctggt tttccttgtg
    13261 tgaatggttt tttaagatta aatactgagt ttactgaaga accgatttgc acggcttcta
    13321 tacaatatca gaagttaaag attgcccaga ttcaagggag tgatctttct cctgaagaaa
    13381 aacaaaatgc aattaaaaag gtgcaggcaa aggagtgtat ttgctctcat ttaggcaatc
    13441 caattcgtat tgcatttgga attgtagagg cgtctaaagc tcctgtttct gtctgtcctg
    13501 gtcctactct tgcttacttt aaagggcctt attctttagt acaaatgatg gcgcatattt
    13561 atggcgatgg cccttcttta gtgccttccg ataggcctca tttatttgca gaagaaattc
    13621 gtttgtatac agagtggttt ataaaagaat cgaaagaggc cactcctgaa tatgcaaaat
    13681 ggcttcatca agcggcactg aatctatatg aaaatatgga ttattgtttg aattttgcaa
    13741 aaacccaaga agctttccct ggagagaatt tagcaagcat tggcaacgcc attacgcttt
    13801 atagacctca attagaaaaa gcacatgagg gattaaaagc taagcaccaa aacctatcct
    13861 agcttgaaaa tgaacaatgg aaggatcttc actattcact ccaaataaga gtgcatggtg
    13921 attggtgttt tcaaatcgaa cgattttcac ttttccttga taaggaatct gagaaataaa
    13981 attgatttga atggagcgta atggtcttgc ttttagttct tcaagagaaa aggcatccat
    14041 cacccaatct gtatagcgac aattattgac atggcgattc atatctaaat cgctgtaaag
    14101 agcttctttt tccaaaacga tttgaggatg atttcctggt tctagaattc ctagcatttg
    14161 aggaagagct tctttatttg gaaagattgg cggagggtat ggagataatt ctgggttttc
    14221 tgctttgccc gtttttaaat tcacaagcaa ccaagaagaa gtgccttgag caatagagtt
    14281 tccttgagca tctaaaataa gataatcttt tagagccact ttatctttgt aatagccttt
    14341 tgtccaagtg gaaatggtta atttttctcc ccaaacagga gtgtggtgta atcgaatttt
    14401 aattcttgaa atagcataag tataaccatg cttcattaat ttccaaatac caaatccatt
    14461 ttgctcggca tcagaaatgg cgctttcttc cataaaacgg aataaattgg aaacacgaag
    14521 acgactatgg tgatcgcaat ccgaaaaacg gaccgtaaag gttttagatg tgatttcagg
    14581 agttgtcatt gataaagagg aggtcgcaag ggtggcctaa aaaacgtaaa cgattactaa
    14641 ttatttttga taagttcaaa agaacctgaa ctcctgtttc tggttgcttt tttaggtatt
    14701 ccagtaattt aatgctatta aaacttagga gagcagatgc ttttacagca cagattcgag
    14761 tagtgtgcgt agattcttca atcatggcca tctcaccaat acaatcgcct gggtggatag
    14821 acctgagttc ttgcatttca ctaccagtag aacttgagac aagggctttt aatttgcctg
    14881 tagccacaac aaaaaaatga tcggttttat ccccttcatc gaagaggagt tcgttttctt
    14941 ttagatatat cataattgcg aaagagctta attcttctat ctgatgttca gacaagcctt
    15001 cgcataaata ggatttagct aaaaagtcaa taattttttt atcgctctct gttttcatca
    15061 tttataatgt ataaatttta actatgaaat cagtcatgtt ttttccaagt tctatttctt
    15121 taccagcttt ttcgatgttt gaaggggttc tccgtttacc agggtctaaa agcttatcta
    15181 atcgtgcttt attattagcg gctttggctc aaggggagac aagattacat catttattgg
    15241 aaagtgatga tactcgttac atgtgttctg ctttagaaca attaggcctt cctattcatt
    15301 ttttagaaaa ctcatctcag gtaaccatta ctggttcagg aggtccatta aaggctgaag
    15361 ctggagattt gtacctaggg aatgcgggaa ccgcgatgcg ttctctttgt gcggctctta
    15421 ctcttgggaa agggaaattc cgtctcagtg gtgaagagcg tatggcagaa aggccaatta
    15481 aagatcttgt ggattcgcta attgatatgg gtgctgacat tgagtatgaa gtttctgaag
    15541 gctatccacc attacttgtt cgagcagatg gtttaaaggg tggtactgtt catgtacgtg
    15601 gaaatatttc aagtcaatat ttaaccgctt tattaattag cggtccatac tgtaagcaac
    15661 ctcttcatgt cattgttgat ggagatctta tttctaagcc ttatatcgat ttaactattg
    15721 atattatgcg tcggtttggt atcctagtgg ataacatgaa ttatcaagaa tttaaagtgc
    15781 ctcaaggagt ttatgtttct cctggggatt tagatattga aggagatgct tcttctgcaa
    15841 gttatccttt ggctggtgct gctattactg gcggtacagt tcgagtagaa ggtgtaggct
    15901 cttcaagttt gcaaggagat gttcagtttg ctgaagtact tcgtcaaatg ggagcaacgg
    15961 tttccatggg agagcattgg attgaatgca ctggcggaga attaaaaggt gtcgatctcg
    16021 atttaaatca tattcctgat gctgcgatga ctgtcgctat cctagcctta tttgcaaaag
    16081 gcacaacgac cattcgaaat attggaagct ggcgcgttaa agaaacggat cgcatagctg
    16141 ctcttgctgc agaacttcga aaagtgggtg cgactgttta tactgatatg acaagtattc
    16201 aagtgacgcc tccagaaaaa cttcaagaag ccattattga aacatacaat gatcatcgca
    16261 tggcaatgtg ttttagtctt gttgctttag gcggtgtgcc agtaaaaatt ttagaccctt
    16321 cttgtgtgaa taaaacgtac ccaaattatt ttgatgattt tttaaggcta gctaaatgag
    16381 attagttctt tttttattga ttcttttaag ctcaagtttt gccgctaaaa aagtggatac
    16441 actctctttt tgggctatgg ataatggccc tggttctgcg gccgtacttc aagaaatgct
    16501 cactcgcttt gaagttgaaa cctctattcc cgtagtcttt aaggaattga agtgggaatc
    16561 cgccttcgac gaaattcaga aagccttctc agaaggaacc gccccagatg tcttgcaggt
    16621 tggttcgtct tggatctcta tatttgcagg gaaaggctat ttagatggaa tgcaatcgtt
    16681 tctcaatgaa attgattctt ctcgtttttt tgatgagatg tccaagttct caagcataga
    16741 aggagactcc aattattatg ctgtgccttg gttcttagat gtgagagctt tttttggaaa
    16801 taaggccgtg ctagattctc ttggaattcg tgatgctcat atgaagagct ttacgcgtgc
    16861 aagaggggtg atgaaagcca ttgcacaaaa tggcttgaaa aatgcagaag ggaaagagat
    16921 tgttcctttt gggcttccag gtaaagacga ttggacagcc cctcaagtca tagctccttg
    16981 gatttggtct tatggaggtg attttctggt gaagcaagac tctctttgga agagtgctct
    17041 tttggattca aatactttaa atggattgtt tcattactta actttactga aagatacgac
    17101 ttgcgttcct aatgccttat ttgaaaattc agtagagaat gccactcgtt ttactaattc
    17161 agaacaagct tttttaatgg ggacatctga aatcattcgt aaaatggatg tatcggtaga
    17221 ggaaggtggc ttaaaagaaa aaagtatttc tcaagatggg cttgttttga tccctatgcc
    17281 ttttggtcca aagggccaat ttacgtttgt tggtggaagt catttagcta tttctaaaaa
    17341 tgccaaaaag gataccaaaa agttacttca gtttttagta cgagtggatc acattgatac
    17401 ttatactcga cgaataggct ttttaccttc agataaaagt gtgattagtg tatgggctca
    17461 agacccgcgt tataatgctc taatagaaga tctcagtcat gggcgtagtt ttcctaatct
    17521 atctcaatgg ggcgaaatag aaaccatttt aatcaatatg agtaatgata ttggttctat
    17581 atatcgaacg gcgattgaaa atgaagatcg tacggcagaa gtcgcaagta tcattgttcg
    17641 tgcagatgag aaaatccaaa atctattatc tacagattca ttagattcat tgagttctat
    17701 agattccaca aattcttcta ttcgaaatac agattcttta ttaacatggg ttatgaaaat
    17761 attgagtcaa aaagaaaaag aagaagattt tttaactgtt aaatggttcc ccaaaggttt
    17821 tatcattcca ttaccaggag aaagttggac tttccgcttt gctattatca ttggaatagg
    17881 tgtcatttgc ctttttattt tctttgtgga atccaagtat catttaattc gtcgaaagaa
    17941 gaaaaagtaa aatgaaggat tgggttgaag aaaagagaaa aatacaagaa ccttcaagtt
    18001 taattggatg ttttaagaaa atcttttttc ttttttcttt attgttttct tctcaggccg
    18061 cctttgcagg tttttctgct caagaatttc aaagctatgt agattctctt atcccaggtt
    18121 ctcgtttagg aatgtcggtt cgttcggtga aaacgggaaa cgtttttttg gatattcgtg
    18181 gagatgaatt ttttacacca gcaagcacgc tcaaaacctt gactacagca actgctcttc
    18241 atttacttcc cttaaattat gagccacaaa cgactcttca tttagagggg agcatcaaag
    18301 aaaaaacttt ttctggaagg cttcgctttt acggtgaggg agatcccaat atctcaggac
    18361 gcttctatac cgatgccttt tacatgatga atcaaatggt cgattcttta aaggctctag
    18421 ggatagatac tattcggggt tctctcttta cagatacgac ttatttttct gggccacgtc
    18481 gtcctgagca ttggaaaaat aattattacg atgcttggta tggcgcagaa gtagttcctt
    18541 taggttttaa cgataattgc actttgattc gcataaagcc aggtgcaaat gtgaatgata
    18601 cggcaatcat tactccttta ccagatattg gttttgttac aataaaaaac gaactcatta
    18661 caacaaataa tcgagcgaga cgttggcgct attctataga taaagaaaaa ccagtgatta
    18721 ctattagcgg tacaattggg attaaagtag attcttctgc tctcgtgttg cctgttcgta
    18781 atccttcggc gtattttatt agcgcatttg aatcagcctt aaaagatgct ggaattattt
    18841 ttattcagga ttattcagct cattcaggga ttgagattaa acagtttaga ttttctgccg
    18901 ctcccttatt gagtatgctc gatgagataa atcagagaag tcaaaattta catgcggaga
    18961 tgctatttcg aaatgtaggg aaaatcgtgg ctggagaggg tagtgtcgaa ggtggtaaaa
    19021 aagcagaaac actcttctta aatcaaatcg gaatttctcc ttctcatttt aaggtgtttg
    19081 atggctgcgg actttctcct ttgaacaagg taaaaccatc tgtgatcacc gagatgttga
    19141 gctatatggc aagatctccg agatcctatt tctatataga aagctttgca tctcctgggg
    19201 ttgggactgc gagtaagcgg atgaattcct tagcgatgcc tcagcaaaca cgtcttaaga
    19261 cgggttttat ttcagaggct catgctcttg taggttatat ctttacgatg actggagata
    19321 ctcttagcgt gagtgcgtat cttaatgata cggggaaaaa tccagataat aaagtgaaag
    19381 atgttctaga tactatctgg tctaggctag tcgaaaaatc aaatgatcat tatccttcat
    19441 tgatgttagc aaagaaactt tggttacaag gtctttctgt aaagggtctt gaaaatagat
    19501 tggactattt ttcaaaagca ctgcttaata ccccttattt attaggccct atgggagaga
    19561 gttatcttga ttctatagat tcaaaaccat taatctattt agattctgtg gattgcgtga
    19621 cttatattga gcacgtctta gcacttgcaa cagcgagtca tgaagactct gttttttcac
    19681 aattgcaacg gattcgttac tttgatggaa aaatcggtta tttatatcgc aaacattatt
    19741 tcgtagaaga tttcttaggc gaaaatgtat ttgcttctgt tcgtattatg gaaggcgata
    19801 ccacaatcat tcgtgaactt ccaaaaaaag atttttttgc ttctaaaaaa atcaagtacc
    19861 aattagataa tcctaagacc gaaattcatt acttgcctta tactaagagt atttcgtttg
    19921 caaaaaaagt ttgggaaggg gagtcggcta tttggggcgt cgcctttgtt ggaacatccc
    19981 caaaaataga cgcgacacat acgggctttt tgattttaga aaacgggaaa aaacctgttt
    20041 tgcgtcatgc ttcccaactt aaaaagaaag tcatagacca aaattttgat gcgtatttaa
    20101 aaagccgaaa ggggaaatta cctggtgtcg tcttttttaa ttttttagaa ctctgaagaa
    20161 aaagcaataa tcatcactaa ttcatattca gtattttttt cgctattaaa aaccgtgaac
    20221 atatctggct tccagtcata gatatccatg gcaagcgttt taccaatcgt cgtttctgct
    20281 ctttctatat cagcccaatt gacatcgttt ggtgtgttat aatagcgaat catcccagcg
    20341 gcttctgaaa ataaagggtg cttttgcaaa agttctttag agcacccttc tgcagaagaa
    20401 gatttgattt taatgacatt aaataaacgc cttggcgata aaaatgaaat caagtcgttt
    20461 gtatcgggtt gaattaaagc attttctgaa aagcatttta gaagttcttg aaagactaaa
    20521 gaatcactat taagcatagg catatacttt tgaatgatcg atgtgatttg cttttgtctc
    20581 ataattcaaa actaactttt tttagctata aaaaaactca aagtttaatt tcttaaactt
    20641 tgagtgaaaa taatcaaatg aaaatcgttt taacagcgct tcaaattaga cttcacgatg
    20701 cttgagatac caagaaatta aactattggt actagaatca tgagctagtt gaatattggg
    20761 gttttgttcg cccttttcaa gttcaggaag aattttgccc gcgagttgtt tacctaattc
    20821 aacgccccat tggtcatagc tattgacatt ccatagaacg ccttgaacaa aaattttgtg
    20881 ttcatacata gcgattaaag cgccaagtgt tttaggagaa accttatcca tcattaaact
    20941 attggtgggc ttgttgcctt caaaaacttt atgaggagca aggaatgcta cttcttctgc
    21001 agacttacca gcagcgcgta gttcttcggc agcaacatct aaagtctttc cgttcatcaa
    21061 tgcttctgtt tgagcaaaga agttcgaaag aagcttacga tggtgatcgc caatcttatt
    21121 atgactattt gcaggagcaa tgaaatcaca agggattaat ttggtgcctt ggtggattaa
    21181 ttgatagaag gcatgttggc cattcgtacc tggttcaccc caaaggatag ggccagtttg
    21241 ataatcaact cgacggcatt cgcgatccac agtcttacca ttggattcca tatcggcttg
    21301 ttggaaataa gcagccaagc gatgtaaata ctgatcgtat ggaagcatgg cataagattc
    21361 agcgccaaag aaattgttat accataagcc taagaccgca agaatcacag ggaggttttg
    21421 gtccatagga gccgttttga aatgtacgtc catttcgtaa gcgccttgat gaagagcgtt
    21481 gtaattatca aaaccaatac gaagagcgat tggtaaacca atagcagacc ataaagagta
    21541 gcgaccacca acccagtccc agaatccaaa catattggcc gtatcaattc caaaatcgga
    21601 aacggctttt gcatttgtag ataaagccac aaagtgttta gcaatagaag cttcgtcacc
    21661 attgaacgct tttaatacag cttctttcgc tgtaatagcg ttagtcatgg tttcttgggt
    21721 ggtaaaagtc ttagaagcga cgataaagag agtttcttct aaacagactt ttttaagagt
    21781 ttcggcgata tgagtgccat cgacgttaga cacaaacaaa acttctggag agccttctga
    21841 agcgtatggg cgtaaagctt ctgtcaccat cactgggcca agatcagaac caccaatacc
    21901 aatattgaca acatatttga tagacttgcc agtatgacct ttccattttc cactgcgaac
    21961 caagttcgtg aattcttcca tgtgttttaa tacggctttc acttctggca tcacatcttt
    22021 gccatcaacc ataatagagt ttttgccttg atagcgaagt gcagtatgga gaaccgctct
    22081 tttttccgta tgattaatct tttccccttt aaagaactga tctcgtttgg cttcgaaccc
    22141 ggtgctcttt agaagatcaa aaagtttatt cagagtctct tcagtgatga tatttttaga
    22201 atagtctaaa aacaaaccgc aagcttctgc actaaatttt ttggcccgag caggatcttt
    22261 tgcaaataaa ttgcgcatat gccatgtttt ggcatcttga aaatgagctt ctaaagcttt
    22321 ccattcatta gattcggtta agtgactcat aatgtttcct taaagaaggg ttaatttttg
    22381 atagtaaatt tagaaaataa aaacaaaaaa gactttgcaa aataattcac aaagcctttt
    22441 agtgtgtttt taggataggt atttatggaa ctaatgaacg agaattcaca ccctattctt
    22501 gttcaataga attcattgtt ggaggaggag ggggtaagtc acgatttctc ggacgaaacc
    22561 cttttttcct atgaagggat ttgaaagaac gatgttgttc tggagttaac gtcgcactaa
    22621 aaaagcgaat gtctttaatg cgttgatcta accgtttttc gttgagctga agcaatttct
    22681 ttttcgcttg ttcgaccgca ttttcatcga tgggttgagc cgctaatgca tcttgcaatt
    22741 cggtttccgc ttgcttaaaa ttctcaaaag aaatacttct taaagaatcg tttaccactc
    22801 gatgagaatc taacttcacc ttttgttctg gagtcagctg aagaatagag tcaagagaag
    22861 aatggagtct tctttctcta tttttctttc tatcggagcg attacgttcc caccgacgat
    22921 tagaatcatc gcgttcaatg gacttagaat cagtggtttt taacgtaaaa gagccttgaa
    22981 aaacagtcgc tccaataaaa aaaccgacgg aacaagctaa aataataacg cttgcaatta
    23041 atagtttgat gcttttattc ataccactac cacctttatt tatactcgtc taagatacta
    23101 gagaactctt ccatagacgc gccttcacca agagaagcat accaagaaag agtttctaca
    23161 ttttcattag attcaaaaga ggaatctaaa ttaccttgat tagaggaaat gcctaagaaa
    23221 atgcccccaa tcacaaacaa aagactcgcc gctacggagg ctatcgaata tagacgaaag
    23281 ggaataattt tttttgcctt cctttctgcg tttatgcgca ggagcacctt attccacgag
    23341 tcttctttag gcattatttt gggaatggag tttaagttta atgaatcagg catagacttt
    23401 ctcctctttt aaaatttcac gaacgtgttg tctcgctctc gataatttgg atttcaatgt
    23461 cccttcgggc aaattaaaaa tttctgaaag ctcttttaat tctaaacctt ctgcatcttt
    23521 taaccaaagc atactgcgcg tttctgctgg gagcgaagat aaaccatatt ctaaaagttt
    23581 ggcatcaaat acatccactt catcggggcg tccaataata ttttccgaaa tactcaagct
    23641 atttaactcc tcaagttcac tatggcgttt tgattttctt attctcatta aacaagcgtt
    23701 cactgtcatt ctgtaaaacc aagtggaaaa agaagcttcg ccacgaaaac tttggattgt
    23761 ttttggcaaa tccacaaaca aatccattaa aacatcttct gccgcatctc gatcttgaag
    23821 aatacgaaag gccaagttta aaatggaaac actatgcgcc ttccatagca ctgataaagt
    23881 atcggcatcg gcttttaaga gttttctttt taaatcgttt tcgttcattt ctaatttaga
    23941 tgcctaagca tcaattaagg ttcccatgat taacaaaata aattttgaga ctttctcaaa
    24001 tgcgatatat ttctgataat tcattatatg gatgattttc ttgaaaattc gcggaaaatt
    24061 gtgttttttt catgagtttt tggacctttt tcgtcgatta tcttgatttt tttttcaaaa
    24121 gactgatatt atattcttct tgacatggaa tatagaaggg gaaagccttc ccctgactgc
    24181 gacccctgcg ggttctccgc caccccttca gcaccccgga cccaggcaac ccccgcattc
    24241 caaaacctgt tccccaaaat ctggtgtggg caatggatct cacgggcatc acagactcag
    24301 cccagaaaac tcgcaacatc cttggcatca ttgatcatgg aactcgaaaa tgcctgtgcc
    24361 tcaaagctct gggagataag gcgtctatta ccttgcttaa aatccttatt caggcaatcg
    24421 atgcttatgg aaaacccaaa gcgatccgaa cagataatga agcggttttc acatctcgtc
    24481 ttttcaactt cggcctttgg ctactgggta ttcgccatca acgcacaaag ttgcattgtc
    24541 cctggcagaa tggaaggatt gaaaggtttt tcggaacctt caagcttgtt gccgacaggg
    24601 ttctgtttga agcgcaaaac ttccagtttt ccctgaatga atttgccttc tggtacaacc
    24661 atatcagacc tcaccgccat ttgaatggtc gcactcccga cgaagcttgg tctggaattg
    24721 acccctattt gcaagcaccc aaatcatgcc gcaagttttc cgcttgggat ggaatgctga
    24781 caggctttca aatggagtac ggctaacatt gccttacccg aaggcgatgc cccactcatc
    24841 aggtcaaaat gtctcttttt cacaccaata aatgttacgc accaaattac gatcataccc
    24901 actcccaaat ttctcatcaa gcgattttca ctaattcggt gggggacagt tatgaaaaaa
    24961 ccaatatttt ttcatgaata actttaattt tgttaaaact ttatttatat ttatgcatat
    25021 atttgcaaat tcgcattaat ggagagtgtg atgtccttaa atttatctga atcatataaa
    25081 aaactacaaa aagagtgtca agaacgaatt cttgtattag atggcgcttt tggaaccatg
    25141 attcagcgtt tgaatttaac tgaagctgat ttcagatctt ctctttttaa ggatcatcct
    25201 gtcgacttga aagggaataa tgatgttctt tgtttaacgg ctccgcatca tgttcaaaat
    25261 attcaccgtc tttatcttga agcaggtgct gatattattg aaacgaatac ttttggtgct
    25321 aatcgtttgg gtcaattaga atatcattta gcggatcgag tctacgatat cgcacgcgct
    25381 ggtgcagtag cggctaaggt ggcggtggaa gaatttaatc aaaatagggt ggctcaaggt
    25441 ctatcttctg ttgttaagtt tgtggcaggt tctattgggc ctacatcgaa aacggcaagt
    25501 atgagtccag atgtttcgga tcctgctttt cgagccatct cttttgaaca gttagccgcc
    25561 gattatggcg aacaagttcg aggcttgtta gatggcggtg tggatattct tttagtagaa
    25621 acgatttttg atacgttaaa ttgtaaagcg gcttgttatg cgattcaaaa agaatgttct
    25681 agacgaggcg agttatttcc tattatgatt tcaggcacga tcaccgatgc gagtggtcgt
    25741 cttttgtctg gtcaaacgtc tcgtgctttt tggcatagtt tatcttctta tcccattttt
    25801 agtattgggt ttaattgtgc tttaggtgca aaagagatgt tcgcgcattt gcgtgaaatt
    25861 aaaaacgcgc ctttatttgt gagtgtttat ccgaatgcgg gtcttccaaa tcagtttggt
    25921 ggttatgatg aaacggccga gatgatggct tctgtgatca aaagctatgc ggaagaaaat
    25981 ttggtgaata ttgtcggtgg ctgttgcggt actactccag ataccatccg agctttttct
    26041 aaggctcttg ctggttttaa gccaagggtg gttccaagtg cttcaaaaag tcttttgctt
    26101 tctggtttag aacctcttac cattgatgcg aatagtcgct ttgtaaatat tggtgaaaga
    26161 atgaatatcg cgggctcttt aaagtttgcg aatttaattc gagaaggtcg cttttcagaa
    26221 gcggtttcaa taggcgtaca gcaagcagaa aatggcgctc aagtgattga tgtgaattta
    26281 gatgatggta tgattgactc taaatccaca atgattcatt tcttaaattt attaatgtca
    26341 gagccgttag tggctcgttg ccctattatg atagatagct ctaagtggga cgttttgctt
    26401 gctggcatgc gttgtgtcca gggtaaaggc attgtcaact ctattagctt aaaagaaggt
    26461 gaaacggctt tcttagaaaa ggcagaagaa attcgtcggc atggctttgc tgtggtctgt
    26521 atggcttttg atgaaaatgg ccaagcaaat acttatgaac gtcgtacatc catttgtacg
    26581 cgtatgtatc gtcttcttgt ggataaaatt agtttccctc cagaagacat tatttttgat
    26641 cctaatattt taactatagg cactggcatg gaagaacatg cgtcttatgg taaggattat
    26701 attgatactt gtgcttacat taaagccaat ttgcctcatg ctcatattgt tggcggcgtc
    26761 agtaatttaa gctttgcttt taagggtaat aatgccatac gtgaaagtat tcatgcttgc
    26821 ttcttgtatc atgcagctaa agcaggaatg gattttggga ttgtcaatgc gggcgtgctc
    26881 cctgtttatg aagatattcc tgaacaagaa cgctctctta ttgaagattt gatttttaat
    26941 cgctctccag aggccacaga taagttgctt gcgtatgcag aaaccatcaa aggtcgtaaa
    27001 ggcaatgcgc ctgatagtgc agagttttta aagtggcgtt ctctttctgt agaagaacgt
    27061 atccgtcatg caatggtcaa gggtatcgat caatttgtag aagaagacgt cgaagaatta
    27121 cgagttcgta ctgcaaatcc agtcgatatt attgaaggtc ctttaatgga tggtatgaat
    27181 aaagtcggtg aactttttag cgaagggaaa ttattcttac ctcaagtggt gaaaagtgct
    27241 cgcgtgatga agcgcgcggt cgctgtttta ttgccttata tcgaagccaa cgccaaagtg
    27301 ggttcttctt ctaaggcggg taaagtggtg attgccacag tgaagggcga tgtgcacgat
    27361 attggaaaaa atattgtgag tgttgttctt ggctgtaata attatgaagt cattgattta
    27421 ggtgtcatgg ttccgtgcga aaagatcgta gaagcggtga aagagcataa gcctgattta
    27481 gtggggcttt cgggattaat tacaccaagc cttgatgaaa tggtgaatgt cgctaaagcg
    27541 tttaagcgtg aaaatctcaa aacgcctatt ttaattggtg gcgcggcggc atctagcatt
    27601 catacggcag tgaaaattgc gcctgaatcc gatgctcctg tgatttatac ttctgatgct
    27661 ggaaaatgcg tgacgattgc agagcagtgg acgaaccttc gaaagagaga tcatttctta
    27721 aaagaacact tgtctcacca agaagaatta cgttcgcttc atgcaaagca gcaatctcaa
    27781 gtgcaaacca tttctataga agaagctcga tcacgcggag agtctttgtt ttagaaggct
    27841 tctcttaatt ttgtgcattc tttttttgtg aacgaacaat agggataaac ttttttgcta
    27901 aagagtcttt ccctctgttt tttatatagg caaagccaaa aagacaaaaa actatggctc
    27961 caataaaatt cacgatcaag tctttcatgg tatcgaataa tccgatatca agatagcctt
    28021 cccatgcttt tccattgaca acaacgcttt ctattggaag agtcacaggg gtgtttctcc
    28081 cttctgggtg aaaggtcaca gaagtgattt catgaatcat ccaatctttt tgcatatcca
    28141 tcttaaaaaa ataatccatg gcaaattcaa aaaattccca aagtacgcca atggtcatag
    28201 aaaaacaaaa agcaattaag gttagggaaa tgggcgtgag ttttgttgaa atcttttcac
    28261 ttcgattaaa tacatcaata aaagaaaaac ctatggcagc cattaaaaag ccgttggtcg
    28321 tatgtagtaa agtatcccaa ataggatatt gaagataaaa ttctctaagt tcacctaaaa
    28381 tttcagcaga aaaaataaaa agtaaaacga caatttgaag tgtgtctgga aactcaattt
    28441 taaactttcg gtcaataaaa gtagggattt ggaacaagat caaagttaaa agacataaga
    28501 atacatcggt gtagttttga ttaaaaaact gaatcaccat aatgccaatg actaaaagtc
    28561 gtaatacgat ataaacagtt aaaggccatg aacgtttatt attaggcatt gttttttttg
    28621 tctttcataa gatagttcca taaaatatgg ttactcaaaa gaaaatagta atatattagg
    28681 gtctatggaa accatggatc tgaacacatt tgtaaaccag cgttggtttt tacacaaaaa
    28741 caattctatt caaaaaggga atgtcgttca tcaatttgat ttatcagatg aatatgcctt
    28801 ttgtgtattc gctttttatt tggattcagg aaaacgagtg ctctatgggt accctatggt
    28861 aaaaaatagg gttgatttag atttaatgaa ttatccagag caccatgctt tatttatgaa
    28921 tgctcttttt caaaaaaaga tttggtcagg ggggaatgcc actcttaaaa ttcattacta
    28981 tgatcaagaa gcgtttgaaa agccatttga aaattctata aaacaaaatt tagaacaaag
    29041 taattcttcg attctatttc aaggtggtcg ttttctaaaa tggtttaggt tcttaaatgc
    29101 aggtgaacac cctgaagtgg agatgggtgt ttttttacag ctgcatcatt caaaggatac
    29161 tgcaaaaatt ttagctcata tttcttatga tgtgaatggc gagtattatg ttgttggtgt
    29221 tattgagagc gctttaccat ttgaaaaaat cgcatgggat tattttggcg aaaatcctag
    29281 tgaagaaaaa gcgtatttgc taggcactca gttagcctcg ttacaccaaa cattattagc
    29341 gatggaaggg gaatttaaga atagagaaaa caaccgcctt gaggtctact ggaatgcttt
    29401 tcaggctaat ctaaaacaaa gtgaatcgca attacaaggg cgttctcttg aagccgcgaa
    29461 aagaattctg tccaaatggt ctttgataga agaatatgta aaatctttag cgcaagtctc
    29521 ttttttggca cccgtttctc gcattcatgg cgatcttcat ttaggccaaa ttttgtataa
    29581 aaaggatcaa ttctatttca ttgattttga aggagagcct ataatccctt tgaatgaaag
    29641 gcgtgaaaag cgctccgtat tatttgatgt ggcttgtatg attcgctcgt ttcgttatgc
    29701 aaaagctttt ttcaaaaggt cttcttctgc ggctttagaa ttggctttct taaaggggta
    29761 tggtgtggag tcttctgatg aacttcgctt tttccttttt cagaagacat tatatgaagc
    29821 atcttatgaa ttgaactcta ggccagattg gttttatatt cctgcagaag atttgcttaa
    29881 tcatgttctt acaaaccagc tatagcggtc tggttttttt ttgaaaacgt gagaataaaa
    29941 aaccccctct ttcttatgaa agaaggggtt tcagtgggac ctcccggact tgaaccggga
    30001 accgacgggt tatgagtccg tagctctaac cgattgagct aaagtccctc aatgttcgcc
    30061 caaagttagt aaatattggg aaattgtcct atcccattcg taacgaaata ggtatgcatt
    30121 agagacaatt ttttcccaaa cgctcttctc attacaataa agggattctg cttctcggag
    30181 acgacgtagc atttgatgag gtgccgcaga atcatctacg agcaaggcat ctgctaaggt
    30241 tgcgtctcct tcgaagtcat gtaaaagatt agcgacacca acatctttac cagaaagagg
    30301 gattgtgccg ctcgccagag cttttaagac aagagatgca gatggctctt gtagatttga
    30361 ggcaaatagt aaatctgatc cagctaagct aactcttaaa gcacttttac tttcgaaaat
    30421 atctagaatg gcgatgtttt gtgggtattc ttgaactaga gattggtagt aattccaatc
    30481 tggattttct tttaaaatac cgacgacaag aaatgcattg agtttggtga tatcagaaat
    30541 aatggtagca agcgtttcag atgttttccc tgactcgtta tccaagtgag tgtatatcac
    30601 taatttgtct tggaaagaag cgtttaattg cgtttcgaag ttctttcgag ccatcttttt
    30661 ggcttcacag ataggaattc ctgtttttaa gttaaaatcc caaagtgcgt agctgacacc
    30721 aaattgaatg cctttaagtt ttttctcatt atggtttaag aaaccactca aaccacctgg
    30781 aagattcgac ttaagcatag aatctctata accaggagaa gggaaaagaa cggttctggc
    30841 gtaacataac cctactttaa gtaaactcac ctttccccag aattcataac catccatgtt
    30901 ataatctttc cgatttaagc caatacgctc aatttcggta tcgtaaacat ggcaatcata
    30961 agtaatgtta tgaacggtaa agaaaaatgg caaatcagaa tagaaatctg aaaaagtggt
    31021 ttgcatcagt gcgcctgtga gagcggcgcc ccattcatgc cctaatacag cactgggttt
    31081 gtaaccaatt gttttacaat aggacaatgc ggcagaggcc aaaaaagaaa aacgaatatg
    31141 gttatcggca taggcgtacc cgttttcacc atatatggca ggccgattaa ataattcgct
    31201 attaaatata taggtatgtc gagcatcttt ccctttccaa atttgaaaag gaagatggtg
    31261 taattcttca attccttcaa aaatacaagt aaatgacgaa aaatcaccta gttgatgagg
    31321 ataaaaagga gaacaagtaa caacttctat acccacgcga gcatacgcat cagtcatatg
    31381 attgacggct tgtacaagtg gacttggagt tttccaacga ccagcttctg ggctaacaac
    31441 taaaaaattc actatataga actccaaaga aaaatttact tatttcttat ggtttgatac
    31501 aaagatattt aattaaatta caaatagaat gtcaaaaaac tctctttttt taagagatga
    31561 atattcaaca agggaaaaaa atgaaaaaaa tgcttttttt ggtctttatg ggcgccttag
    31621 tggctttaac aggctgcaaa ggaacaaatg aaaaacgagg ggacgagcat cttgaagcag
    31681 gccgtttccg taatgccatc aattcgtatt tagaagcgaa gaaaaagggt aaaatttccg
    31741 atgaatttta tgataatttc gctctcgctt taattcgtgc agccgatgcc gaagcaaaga
    31801 aagatctgaa tagtcagctt ctttcgggtt attttgaaca agctcaaaaa gctttagatt
    31861 cagcaaaaaa agacgaagtg acacaagaat ttgtgacagc tcttgctaat ttaggtaaac
    31921 ttcaagcggc tcaagaaaca gactacggta ctgtattgaa cgcttttgct aagttagaca
    31981 ccgccatggt gatggcaaaa cgtaaaggtg ttggcgaagg cgctgtgaag accattcgtg
    32041 ctgaagctga aaatgcatac gttaaggttg cattaccaaa tgctttaagt gaagaagatc
    32101 ctgttgttcg tgagtacttc ttactccaaa ttgatgtagt ggctccagaa aatgccgaat
    32161 taaaagaagc attaaataaa agccgcatta acactcgagg ttatttcttg attttcggtg
    32221 aaaatatcgg agaacgtcct tcaaatcgtg tggataaatg gggttatgtc atggctttcc
    32281 catccattaa gatttcttcc acatctttaa ctggagaact tcaagtttgg gcttctacag
    32341 gtaataacac agaatttgtc gtagacaaaa tcaaattagt ctctactgac ggtaaagaag
    32401 tttttgcaaa acagactggt tcaggctggt gtgaagcaga agtggttgtc ggtaaaaaag
    32461 gcgacgaaaa ggtggaaaag aaaaaggctt catttaaagg aaaaggtaaa ttgctaaacg
    32521 aattccagtg ctcagcgaac atagcattta catttgatag cagctttatc ccagattatg
    32581 ttgaatataa agatgaaatg ggtattggcc gtaagtattt aggtcgataa tcattttcta
    32641 ttgattttct cacaaagaga ctcgttttta gcgggtctct tttttatttt cattataaag
    32701 gctccctcaa aaatccactt tactaaagct tttttatttc agaggaatct aaatatgttt
    32761 tatcttggtt gtcatttatc ttctagcgat ggcttttatg ccatgggtga aacggctctt
    32821 agtattggag caaatacatt tcaatttttc actagaaacc caagaggtgg cgcagcaaag
    32881 gcttttgatc cagaagatgc tgaaaggttg gtgcagtttt tggataagca ctcttttggg
    32941 ccaattttag ctcatgctcc ttatactatg aatccgtgcg cattagatag tggtttacgt
    33001 gaatttgcag agacgatgat gaaagatgat ttgtttagaa tgaatcattt taaaaaaagc
    33061 atgtacaatt ttcacccagg gagtcatgta aagcaaggcg tttctgttgg aattgatttc
    33121 attgcttcta tgctgaatcg agttctttct ccatcacaaa atacattggt tttgttagag
    33181 acaatgtctg gtaaagggac agaagtgggg agccgttttg aagaattaca gagcattatc
    33241 gaaaaagtag aatgttctga aaaattaggt gtctgcttag atacttgtca tgtttatgat
    33301 gcagggtatg atattgtaaa tcatttggat gatgttttag atgagtttga taaaattatt
    33361 gggctttcta agttgaaggc aattcatttg aatgattcca aaaatccttt tcaaagccat
    33421 aaggaccgtc atgaaacaat tgcgaatgga tccatagggc tagagggtat tcttcgtata
    33481 atcaatcacc caaaattaaa atcattaccg ttttttttag aaacacctaa tgaacttcct
    33541 ggttatgctt cagagataaa gatgcttcga gagcgatatg aaggctaaag agctacgtct
    33601 tttcttaaaa gaaaatgtcg atttgaagta tcaaaaattc agtgcttcat tgttacctgg
    33661 agtcactgat ttgtggggtg tgcgtttacc tgttttgcga cgtatcgcca aaaaaatagc
    33721 gaaggaaggg aatgttgatt tattaaaatc aagcttaaaa aagccctgct ttgaagaacg
    33781 tatggtgctt gggatggcta ttggttattt gccttgggat gatatttttt tagaaaagta
    33841 tgtagaacct tatattcctc taatcgacaa ttggtcgatt tgcgacagct tttgtgtggg
    33901 gctaaaacaa gtcgcgaaaa atcaagaaga aacttggcac tttttacaaa aatattttaa
    33961 gtcggattta gaatatgagg tgcgctttgc cgtggtgctt tcgttatttt attttttaac
    34021 agattcttat ttctctttag ttttagatca attggcgtta attcatcaag aagattatta
    34081 tgcgcaaatg gctatcgcat gggcaattgc gaaagcgtat gcggtttctc ctgaagtcac
    34141 taaaaaatgg attaaaaaaa caaagataaa acaagagata ctcaaaaaag cattgcaaaa
    34201 aattagagat tctttggtta ctaaatctag cgcttctgct tttgaatgtc taatataaag
    34261 gcaagtagcc ataataagaa gcaagcgaat agagggaaaa tgatattcca gggcatatgg
    34321 aggtgaacgt attttggaac aatcagccaa atatagatga gaatggtatg ggccgtgtag
    34381 acttctaggc tgtgctttcc aagcgttgcg gaaaattgaa aatcaagtaa tgaggggcgt
    34441 gttctaacaa taaagcagat gcttaaagca aaggcaataa agttgaggta acgtagaggg
    34501 cctagatgtt ctcggctaat ccaaaactcg ctcggtaagg gaagtgaaat ccattggtga
    34561 gaccagagga acaaaaaaat tgtaataaaa atggctacat aagaaatgtt ttgtacaaat
    34621 cgatgaggcg ttgtctttgc tttttgatgg tgtttccacc acgcagcgat tgccgctcca
    34681 gagaagtaga cgtactgcca tccaagtata tcaaagaagc cgtggttaac ccattttggg
    34741 aaaagggcga gtatgttttc tcgaatacca aattgggcta aaatccagac taaaaaagag
    34801 agaaaccaaa ttttataaac agaaccgcga gcgaatcttg gaaaaaagaa cgatccgatg
    34861 agcattaaaa ttacatagag aggtaaaacg tcaagccaag taggggtgtg gattaataag
    34921 gctgaaagca aactacctgt atgtggatgc tggagaatgc ctctaaaata gggcactaag
    34981 gcgggtaaaa ataagatcga ggaacagaca agacaggtta aggaaataat atggtatttc
    35041 caaattcgaa atgctctagc tcgcatccaa gaggcgcttg ggtctttttc tcttttgctt
    35101 atggcggcga acatgccgac aaagcccgat aaaaaaaaga aaccttcggc tgcactgaaa
    35161 aaaccgaagc attgaaataa ataagatgat ataggttttc caaaatgatc gagggtcatt
    35221 tggagtaaaa gtaaccctcg aatagaatct agtgccctaa gtctcacaag aatttttaga
    35281 aattagtgtg tccgctaata cccattgaaa aattattagc ctttttaggt aacacgttat
    35341 aaccgcccca gaatacgatt tcagaattga ttgtagggta gaaataaaga tttggtccaa
    35401 taaattgacg atgatcgctt ttgattttgt tacttggatc atggtattca taggcgatac
    35461 ctaatgatac tttctgatga agatcaatag atggttctat gaaaaataag gtttggtctt
    35521 ctgtgaaaat ctgagcttgt acagaagaat ctctttctgc aagtagagct tgataaaatg
    35581 tggctccgat attgaaaaag tcataattaa aagaaggttc tacaagaaat gagtgaacgc
    35641 cttcgccttt gtatggatga gttccccaag tggcggtgat gccgtaattc atttcccctt
    35701 tgctttttgc ataaacagct tcgagtccaa gagcgtaggt ataagaacgt cctatatcag
    35761 aaccaaaagc ccattcgatg ctaggagtta agactaaacg ttcttcagta cgagaaagaa
    35821 gttcaacggg gtatgagcca aaagcgagga tagaggacga attgttctca gaggcaccga
    35881 tataaacttt tcctccttct aactcgacgc caataccacg aagacgttgt tcttttgaaa
    35941 tgacgttata acgactggaa tctctccaga agtaataatt gaagctacca gcactataag
    36001 aaagatcgcc aaaaacggta gttacgttag gattaatttg ccagcggaag aaaacaccgt
    36061 caaatttaat attggtaaaa cgattagctg tatctcccat cgcagtggct ctgctttgcg
    36121 tatgacgaac ttcagaagct tttagtgctc cgagggagtc catgtaggtc ccttgaatat
    36181 tggttttcac tgtagcagag aaggattcgt ttaaaagacc tgtaagagaa aggtcaatat
    36241 cttggtttgc ggcattagtt gggctaaagt ctttatccca ataagttgca taatcggcat
    36301 taatatttcc attaacttct atatcagcag cttgagtgaa aacagctgtc atcagagcga
    36361 taggcaaaat tagtttgcgc atgtgaatcc tttctcgaaa gataaatcgt tcgtgcaaag
    36421 atacaaatat atccactctt agcccacgag aaaagataaa aaaggatggc tctatggagt
    36481 catcttaaca aaagagctta attttttaaa agaaaaaggg aatatcctat ttttgtatat
    36541 gtcagaattt gta
//
LOCUS       DAOOBB010000002        29289 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_2071_length_29289_cov_4.939454, whole genome shotgun sequence.
ACCESSION   DAOOBB010000002 DAOOBB010000000
VERSION     DAOOBB010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 29289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29289
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2071_length_29289_cov_4.939454"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1377
                     /locus_tag="PLT31_00165"
     CDS             <1..1377
                     /locus_tag="PLT31_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPW93576.1"
                     /translation="IVIGHTIMAKGAIAEDGTSYEGQVSTHGQPLNAAGASTEATVAR
                     LGGNPQDPFQIFPEVKKAFASRLDELRAQVKEWEKKKSTWDKENAELSARLDSWLSGQ
                     GPRLNLSDLPIKEGVATRVSSGSVLAHLAENYKNIICSSADLSNSDNTQAFLDRTGIF
                     RPNDFKGAFLQVGVAELTMGAIACGIALHGGLYPICATFFVFSDFMKPAIRMAALMGL
                     PVKYVFTHDSFRVGEDGPTHQPIEHETQIRLLEGLTKANGKPEMLVLRPADAYETLVA
                     WEMAFENTNSPTALVLTRQNVDVLPSEGVSHYERATKAKSGAYIVSDNTKPNAKPDLI
                     LVANGSDVLLNHKAAEILRAEGLSVRTVSMISPKLFLTQPKSYQNQVIEPWRPVMALS
                     SGLPLLFQSVVGGMGKTIGLERFGASAPAPVLEKEFGYVPEAVAEKARAYLAEYKQAC
                     SDFLKANT"
     gene            1599..2531
                     /locus_tag="PLT31_00170"
     CDS             1599..2531
                     /locus_tag="PLT31_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93577.1"
                     /translation="MRTIIFFTLLFANTLMAQEAPDNIRNLCDTLRIGETTEERTMKI
                     SLDTIYDFRFENDNQINSHFYRSIEDLSPIRGSKNFADLTIPVSIESQCLKSNNACSI
                     AFYDADAKKWAAKDTSCVTQIYDIHESLFDVYDSSNSYNWIQYRFEDWSANDPMNQTD
                     ILLSVTPEMRASSWLTIAYVVDTVDGGGFRYSLGMSRNVDSLESATGAIDLLAAAKDK
                     VIYRQTLKFVLAEEKLIVEDPPISIKKSIENPRPSFSMQRMDGGVLIRGPKGEVPSLR
                     LLNGTSVSGKVPVSAGVYFISVEKGSWHRIFVQD"
     gene            2655..4391
                     /locus_tag="PLT31_00175"
     CDS             2655..4391
                     /locus_tag="PLT31_00175"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HPW93578.1"
                     /translation="MVNRSLLLVFFFGLAFVFAGDPKLSLDFVNTDIKEVVRTISAAY
                     DMAILVDQNISANITVHLDSVGLFEGLSAIASANGLEVFQEGSLYRIRKKIERGENYF
                     SSKEGLVSIDVQNKGIQEFIKEYAANTNLNILIDPNIKAKISGRLEGLSPFSALVSLM
                     QAHGFRVLQKENFFLIEEDRHSREEGASSVLDLAYRNSSFFVDLQSASLKQVLNEMAT
                     LAHLNLAVYGELRENVHLKADSLSLKALLKMLFQGSRYTYTFDSLGSILVGERGAPSA
                     IAGNSLYYLEHIHSEKALLLLSKAYQDRGLRILEVKEQNALLLMGASTEIESATQFLK
                     QIDVENLQVLLECIIVEFNKGKQFEFGLRSGSGKKTAEGGLGLNSFIRITDKSRSWGR
                     GFGEIGLLSDQFDFELAALEENNQAEILARPSISTLNGNKASINVTNTTYYMVSQVSA
                     DGFPITDYRSFNEGVSLEITPSVARSGEITIEIIPEIKTSARSTGEGPRDISTRNLKT
                     IVSLKEGETFCLGGLIRKTSAKIRSAIPFLGSLPWIGKWFSYHTNEEYTMELAVFITP
                     RLIRKGMNKRTP"
     gene            4388..5485
                     /locus_tag="PLT31_00180"
     CDS             4388..5485
                     /locus_tag="PLT31_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93579.1"
                     /translation="MILVKREKEMVQGVSECFTLTLELQTPFGEEPHPEMILDHIKKE
                     LPLSCERAQNRISFLPLISFNSSMGRTHRFLVAFSLKDFSKNRSFCDRVLPLGIAFSA
                     FAEEEMINKDGYENLICGGVLGDHFFLLIFYNGKLVHWIQEHIESKTYDLNKRIHRLR
                     LFIQADPFLSRLGDCPISILSEEKHYSFFEKAINDPLWRRFDLDPSVELKERAWKRLQ
                     RRFIYAFILLLIFFVSTFFLNQFLFSSKEMILAKQAKDELYQDAERQKILAFQNQILQ
                     LPFPLDLDLLLQEIGNAVAKGTRLKALKKIASEEGDYYLLNFSAIHFEEASRLENKLK
                     NSTVFKVKNIRVGPVQSTTDGRVAFQMDLFL"
     gene            5482..6288
                     /locus_tag="PLT31_00185"
     CDS             5482..6288
                     /locus_tag="PLT31_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93580.1"
                     /translation="MKFNQQQKIVLWLLGIGVFFSLGSYFILYPTIHNIFEKRELQKK
                     QLRQPISFTSWDSIRGSWERLENSFNHSLFSPDSLVTLALESSLGLKQLKVLASGNEY
                     RFHIEGNFDSIYSFIQAIGIKAPYWAFEQFELVKSSKPNLYYLKNVCLNHVHINSNEL
                     KDHIIQELEVKMKIKKMISFRERKKYLNFNPFENERVVPIKVTRKSQSCRLPLFQIKG
                     VVARRVLHVEQEGSKIIYRMGDFIGDYRIQNIETKRILLICGQDTISRYL"
     gene            6285..7142
                     /locus_tag="PLT31_00190"
     CDS             6285..7142
                     /locus_tag="PLT31_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93581.1"
                     /translation="MKNQKSGAILPLVCLLFLALSFIFCALLHSTRVLMPRVTYYEKC
                     LQEQYDAESAILLSLFRVPASQSKKHYPIVILDTLGPWLVLKSKAGEREVKAFAGFYS
                     SVKTPPFSRLELEQAETRCMLFYQEQWRKESYIDTLFGTTHYYKPQIKDFPGVLYVAA
                     GDLSISLQGERFSKVAFYVEGDVLIEGEGILDSLSLYATGVISLSGQIKIYDANLYAR
                     GIINISDQIEARGVFFSEDSVVLPFSKSVGLPFWSNSRDDIENKDSTFYAVDLAHENP
                     LRIFSWSVE"
     gene            7139..7489
                     /locus_tag="PLT31_00195"
     CDS             7139..7489
                     /locus_tag="PLT31_00195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPW93582.1"
                     /translation="MNKGFSIIETLISLILISVLSTSLLFFLDGYREIQSLEQKRFQA
                     FREASAWMEAEIDSPPFCLDYAKEMPLETWGSIKLSRTRITEKDPLVLIQINVFSKEN
                     KRLFSSLERVVECE"
     gene            7480..7752
                     /locus_tag="PLT31_00200"
     CDS             7480..7752
                     /locus_tag="PLT31_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPW93583.1"
                     /translation="MRVKKGFTLLELCVGVILTGIVFSISFSYFYQMHRISSDLVKKQ
                     LDDLEYSIQVLQEEERLRSKCILFYTDKEEKIKTLYKLKAANRCRF"
     gene            complement(7782..9329)
                     /locus_tag="PLT31_00205"
     CDS             complement(7782..9329)
                     /locus_tag="PLT31_00205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPW93584.1"
                     /translation="MNQLLLNLMFVVYVIAGIGLIAYGFNCYVSIYLFLKNSRRVRLT
                     DRKNILRFYRDHSMDDLPMVTTQLPVYNEANCVERLIDAVCAIDYPRDRHEIQVLDDS
                     TDECLEVTTRKVNEMKAKGYNITLIHRTNRKDFKAGALKEGMAIAKGDFLAIFDADFV
                     PEKDFLLKTVPYMVMDPTIGLVQGRWGHLNRTESGLTLAQSIGIDGHFVIEQSARSWG
                     NLFMNFNGTAGIWRRQAIDDAGGWEGDTLTEDMDLSYRSQLAGWKMKFVFDVIVPAEL
                     PNDINAFKAQQYRWAKGSIQTAKKVLPLVMKAKVPLRVKIGAFMHTTHYSIHPCMLFT
                     AICAFPLLAWFEPIAQVPNFVFAIAFTFIFLAAIAPSTLYFVAQRCSGYTGWKIRLLS
                     LPILMAVGVGIAVSNSRAVMSAVLGHKGAFVRTPKKGQKHLSSYAQKFPIIASLELLV
                     GGYCIFGLLEYIGAQKFIIGPFLALYAIGFLSVGVLSFMHYFTNLIEVHRDRKAQAQD
                     IEVQPEA"
     gene            9430..9966
                     /locus_tag="PLT31_00210"
     CDS             9430..9966
                     /locus_tag="PLT31_00210"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HPW93585.1"
                     /translation="MISTPIQKWLHVNRNLPDFDKALQSLLGHACVDILRRNFPNSPV
                     FSLEKAFSILSLYYQEELKVPLLDFMSEPLQHASQIVDFCEKTSTFSFLKKFPEVDFL
                     NEKLDLRGVVCPGNAVRAKLVLAGLPAGSTLQIALDEGYPIQNVPQTLVAEGYFIKNR
                     EKKDDYWIITVLKEEEPK"
     gene            9976..10557
                     /locus_tag="PLT31_00215"
     CDS             9976..10557
                     /locus_tag="PLT31_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93586.1"
                     /translation="MENRILVLADDFFGENGEAASHFSELLLCLAPDLSVQFVLLDPV
                     LIPLETLLQRAPSDIIGKQAGRIVLGLGLRELRLHQKASLIFKTYKALLDELLSKTKA
                     HLNLVTLPEESFKEEILQVSEWNELLKSISHPRITVLDFADRAAQFKETQLQKGKFAR
                     RLYSEDSRATSLCHMLLGLFLQERILKEIKEIS"
     gene            10554..10886
                     /locus_tag="PLT31_00220"
     CDS             10554..10886
                     /locus_tag="PLT31_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93587.1"
                     /translation="MSDFERFSAKNAAMSRAWEMDSVEKKKEKKGHHSSEPVSGICDK
                     CKKEAMVKSYRFRQTDRTDGAASFGSVVKHLCPECAPKDKRNENAPPPPTDRQVKNLL
                     RGALRKLR"
     gene            10991..12898
                     /gene="sulP"
                     /locus_tag="PLT31_00225"
     CDS             10991..12898
                     /gene="sulP"
                     /locus_tag="PLT31_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HPW93588.1"
                     /translation="MPKPSFFVSIKTFVSGFSGVCTPELYKVLKKGYTKSNLMSDAMS
                     GVIVGILALPLAIAFAIASGVGPEKGLYTAIIAGLLISVLGGSRFQIGGPTGAFIVIV
                     FGIVREYGYDGLATATLMAGVMLVIFGLAKLGGIIKFIPYPVTVGFTAGIAIIIALGQ
                     VPNFLGLTFPEGVKEPVEAWGKIKLYASSITSFNWVSFLIGGLALVICLYWPKLTNKV
                     PGSLIAILVTTILVKTLGLDEKFGVITIGAKNHIPTGFPIPELPNISIEMMRKVFQPA
                     LTIAILGAIESLLSAVVADGMTGSKHRSNTELVGQGIANIFSPIFGGIPATGAIARTA
                     TNIRNGAFSPISGVVHALVLLLIMLIFGKYAEMIPMAALAAILFQVAFNMSGYRSFIK
                     LFKAPKSDVTVLVMTFALTVLFDLTVAIEVGVLLAAIFFIRRMSEVAQVDFVTQALKE
                     DDEEISDSYNLGSRQVPKGVVVYEIAGSIFFGVVDKFKETLEQVADNPKILILRMREV
                     HSIDATGIQMIEELLIRSKKQNTQLLLSGVHTQPVVALTRAGVMSMIGEENVLANIDA
                     ALNRAREILGIEMIEPSEMEESPSVSWEKNLDKPWLPNHANGSVAESTSEVIAEKVAE
                     ETESKETESKE"
     gene            13035..14879
                     /locus_tag="PLT31_00230"
     CDS             13035..14879
                     /locus_tag="PLT31_00230"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HPW93589.1"
                     /translation="MYFTLIVLLLAMVFFIQGKIRSDIVALSATVLLMLSGILSVPEA
                     LSGFSSSIVIMMLGLFVVGGGVFRTGLARWLGSRIVSLAGTNETSLLLVIFLVTAFMG
                     CFVSNTGTVALMLPIVVSLAANAKIGVERLLMPLAFASSIGGMFTLIGTPPNMVIHET
                     LLKAGKNGLLFFSFAPVGAVALVVSLAMLIPLSKLLKGKNKNKDKNKASQKSLNQLAN
                     EYQLVDSIYRVRVPANSPIVQKKLIDLNLPLQYNLIVTEIRRHSRSSRLLMKEVQQEI
                     AGAGTVFFAGDTIHLLGASKDVAFFAKENNLQLIESEEGNNKSFQFFKVGIAEVLLLP
                     DSFLENKTVLDAQFRTRYGVNILCVQRKGNYIVKDLKSLPFLSGDTLLVQGSWDDIRK
                     LSEEINNWVVLGRPLEEAAKETLDHKMPLAAFIMVAMVVLMVANIVPAVTAVLLAAIL
                     MVLSGCFRNVEDAYKSINWESIVLIAAMLPMSIALEKTGLSASLSSALVSSVGSYGNY
                     ALLAGIYFTTSLITMFISNTATAVLLAPIALTSAAGLGVSPYPFLFAVSVGASMCFAS
                     PFSTPPNALVMNPGRYTFMDYIRVGLPLQIVMGIVMVFALPIIFPFNP"
     gene            complement(14876..15901)
                     /locus_tag="PLT31_00235"
     CDS             complement(14876..15901)
                     /locus_tag="PLT31_00235"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HPW93590.1"
                     /translation="MNIQQKAYSRFESIGLPKARHDDWIFFPNSAFQKINVISPEENF
                     SESISKEFDLGIDTEKNIAALIPLIHTRNTFLKTIDANAQETGVLKARDDFSHTLFHI
                     QKGADVSLEILDNKNEHAFTAERLDFFVEENAKLELFFCNPLQNHSLQFRHVRIVAAA
                     KASIKILGLQENTGCYRQSFDLFLNGEDSTVDFRLLNQLKGETEAHYHLTIHHNAPHT
                     KNNQFVRQILNGKSHVSYDGAVHVEKDCTEVNSAQLINSILLSEEAKVSVKPVLKIYH
                     DDVECTHGNTCGELDAESLYYLQSRGIDAKKAEEILLLNFASEVFSDLESSIGRERIL
                     HILANKN"
     gene            complement(15901..16647)
                     /gene="sufC"
                     /locus_tag="PLT31_00240"
     CDS             complement(15901..16647)
                     /gene="sufC"
                     /locus_tag="PLT31_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HPW93591.1"
                     /translation="MLSIQNLKTSLKDGTSILKGVNITVQPGEIHAIMGPNGSGKSSL
                     FKAISGHPSYQIDSGSVILNGEDILGMDINNRANAGLFISMQYPIEIPGVKNVDFLRM
                     ALDSKRAYLGLEKIKDSEFNQLMEKYTSLLEMDDRYISRGVNEGFSGGEKKRNEILQM
                     AILDPKVACLDETDSGLDIDALRIVARGINEMMTPEKSVILVTHYQRLLDYVKPHFVH
                     VLRQGKIILSGGPELALKLEEQGYDWIEEA"
     gene            complement(16666..18537)
                     /gene="dnaG"
                     /locus_tag="PLT31_00245"
     CDS             complement(16666..18537)
                     /gene="dnaG"
                     /locus_tag="PLT31_00245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPW93592.1"
                     /translation="MAFYNDDLIQQLKAHADIALIIERFVPLKKSGVGRFIGKCPFHD
                     DRSPSMNVNPQLGIYKCFACGAGGDVFKFVMEHEKLDFKAAVEWVAQESGFSLPVLGE
                     PEKAEITEEKEFVRHLNELAATWFENQLSLNAPALEYLAKRGITEETRQEFRIGYAPE
                     NPSDFISLAAKEGFSPRQVVKAGLAIEKENGGITDKFRGRLMIPIHNLSGTVVAFGGR
                     IMKDIPRAPKYMNSPETILYSKSDILFGLNHAKNFIAKEDAVILVEGYFDLISLYQAG
                     IKNVAAVSGTALTEMHAKILGRYAKTAFLVFDGDTAGKKATMRSLEILLPHNIAPRIF
                     SLSRPNGEKIDPDNFVRENGASAFLNEIKQALDWLHYLSIEKETQSPEEKAVFVNHAK
                     SLIAAIQDVELKEQYLNLLSERFNTSRSLSHVRPVAVKKKAVEELPAIDNTPQVPWNI
                     LSGVELRFVNLILRNNDLWKPASRFFSLDFIAQNLPLLESSLLEELLGDGLALYAEHQ
                     TIDLKILHSIANPPLPEILEGLPEEIWSPANAQKEFLETLLILFKRQCDRFCNELKQK
                     ENEESTMLRLEINAFSKKQQKLLRESNLGHINSSDLFHQILENRTLLLEFHHKIQEG"
     gene            complement(18563..19888)
                     /locus_tag="PLT31_00250"
     CDS             complement(18563..19888)
                     /locus_tag="PLT31_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPW93593.1"
                     /translation="MQVKDRSLFSLSWPLILTFGVGMFQPIMDSWFLSRTSEVAAAGV
                     GAVLPILGALFMAIQAFSQAGASIASQYLGGNYSRHARVTQTMIIIGSFLLGISMTLL
                     ILPFSSSISRWIGLEPTPAAYAEQFLFVTAFGFAFRALQTTYTALIATHGLTIWNLIG
                     NIITIASNAFLNVVFLEGWFGLPQLGVHGVALATAISWLISAIFLGFILKTKIHHKNQ
                     RSDFKRIRVILPDWIRIGLPAAAEPFSFQLFQIFITAMVVSLGTIAMTSRIFSANLAA
                     LAVILSVGLGSGNQILVAHLVGAHDYNKANRRLHQSLIISCISSFLIALSVALFGAHL
                     MTFFTDNPEVIRIGAACLWCDVVLQPFKAANIVITSSLRAAGDSSFPAIVGSGMMWTL
                     GVGSALFLAFVLDLGIIGLWLGMACDEFYRSIVNYIRWRKGRWQTLGVM"
     gene            complement(19992..20474)
                     /locus_tag="PLT31_00255"
     CDS             complement(19992..20474)
                     /locus_tag="PLT31_00255"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HPW93594.1"
                     /translation="MKMKAKTVQESIIETNDIVHPSDVNAYGFVFGGHLVSLMDKTAC
                     MAAFRHCESKVTTVSVDGIHFLRPAPVGTMLTIHASVNRAFRTSMEIGLKVTAWLPGK
                     PNEDICRAYMTFVAIDEKGRPKEVPAIIPETEDEKRRYNNAEIRRAARLELREKLLSK
                     "
     assembly_gap    20537..20546
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(21189..22880)
                     /locus_tag="PLT31_00260"
     CDS             complement(21189..22880)
                     /locus_tag="PLT31_00260"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPW93595.1"
                     /translation="MKLSRYFYTTLRETPSDATMASHIFLMRGGYIKPLSTGIYSMLP
                     IGHRVMKKIENIIREEMNRVGGLEVDLPIVQTAELWSESGRYSAIGDELLRFKDRNNH
                     NMVLAMTHEEAVTDASRYVLSSYKQLPFMLYQFKTKYRDEARARGGLIRVREFLMKDA
                     YSFHATQADLDAYYEEQYQAYLRIFRRVGIEPVVVQSDTGIMGGKLAHEFMLDTPSGE
                     DYLILCKKCGYQANREIAKFQRHPYTNNAEAALEKVATPNKESIEEVSSFLQVSPEAT
                     AKCVFFDIEGKLVTVLVPGHLEVSEIKLRNILKTKELIPADESLIKASGMEPGFASPI
                     GAKNTRVLIDLALENANDLVVGANEKDFHFKHCNPKRDFSNYEVLDLAEASALCHCPN
                     CDEMLTETRGIELGNIFKLGTKFSESMKATFLNPEGQSEPAVMGCYGIGVGRLMASVV
                     ENSHDEFGPIWPKSIAPFQVEIVSIGKEANVIEAADKLEKELTSLGFEVLLDDREERP
                     GVKFKDADLWGIPVRIGVGKKGLEQGQVEWKLRSEKNFEMVDLDQITEKLRLFYN"
     gene            23009..24214
                     /locus_tag="PLT31_00265"
     CDS             23009..24214
                     /locus_tag="PLT31_00265"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HPW93596.1"
                     /translation="MKLKCLILLMLVLTMLSACGTKETGNSTISSNSTDEQKFSYMLG
                     VQFGRQAFRLVPQQMGHELEEDYTVQGALDAALAYDDTTKAIQITNDTLRAVSERFSN
                     VARERLEKARPDSATIANSPDPRQLRHISDSLLKLLPVEPAKPGTGKVVKVSDSSSDL
                     EKFSYMLGTEFGTQFGSIGSQLKVTLSPDAFKVGLLDGAKAYRDSSYQFQLPEDSLRA
                     VGRRFSEKGRAAHEEAQKKMMEEEAKLKAKAAAYVGDTLPDGTPAKINFTIKMSALNS
                     KIESFAPYAGKPLFVFYYSTTCGHCRNTAPKVEEFAGKYKGEGLTTVAITTGGTPKRN
                     ILRFSDEFKIQNMEILSDDERLFGQLYGDGYVPKFYLVNPDGTYKRYASFEDNFNEIE
                     ADVKALLKK"
     gene            24211..26076
                     /gene="mnmG"
                     /locus_tag="PLT31_00270"
     CDS             24211..26076
                     /gene="mnmG"
                     /locus_tag="PLT31_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HPW93597.1"
                     /translation="MSSIIAEYDVVVIGGGHAGIEACHAAWKLGVKTALLTMSIDAIG
                     RMSCNPAVGGISKGQIVRDIDALGGLMGILTDKAGIQFRMLNKSKGPAVWGPRAQCDM
                     NYYSELARDTLTALPGLSLIEGELASFSRLPDQRFELILLDGSRYISKTFVITTGTFL
                     ASKMFTGNDTSVGGRVGEASSDKLSENLISAGIALRRLKTGTPSRLDPDSLDFDECEV
                     QHGDEDPWSFSDREQVPLNNNGVCWITRTNLKTHEILRSGFKDSPMFSGRIHGKGPRY
                     CPSIEDKINRFGDKDGHQLFLEPETRDIGRVYVNGFSSSLPADIQLAALHTMPGLTRA
                     KVLQIGYAVEYDSVDATQLNPTFECRHYPGLYFAGQVCGTSGYEEAAGQGLMAGINAA
                     LKVKGEEPFILGRSESYIGVMVDDLVNILLDEPYRMFTSRAEYRLFLRSDNAEVRLKA
                     KAREIGMISDRDWQDWITRETIMRDVHDFLSKESGTPTQVNPILEPSGQALVSDRVRW
                     IQVLRRPGIDPEAFFKVACPDKKMTRRDQWFLYSEEIYAGFFERQLREIEHQKKMEKI
                     RLSPDIDYMSISALSIEARQKLMAQKPLTMGQVSHIPGVRQADITVLAHWFETSK"
     gene            26238..26543
                     /locus_tag="PLT31_00275"
     CDS             26238..26543
                     /locus_tag="PLT31_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93598.1"
                     /translation="MSDTEKNKQESKPQETKSQEKKAPAKKKHSNAGRSSKANHPFKK
                     GAPSNKKPVTFADSLEDRFPALAAKLKKHVEDGIKRKIKDAQSDPKVQKASAKYGAN"
     gene            complement(26722..27480)
                     /locus_tag="PLT31_00280"
     CDS             complement(26722..27480)
                     /locus_tag="PLT31_00280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPW93599.1"
                     /translation="MAYFYFEASEEDIDQCNIMFSLDICQTVDLAGFNKACSNDKLIQ
                     ENLLPEKVDESYNKENKTYTWALPIVIDSLSLGSGIRVDIVASSGTKIENKCLTYNIP
                     TEVDISQSWSLTEHSITDKEPIYYAGAPSWDKDELTPAPKAPYIVIRSKASLLWGYGP
                     FDTTNDRVGIFYMPIASKALIQVNSNRLFVEAEANGSKTVKIFDLLGNELLSKTFEGS
                     STEINLAPLAHRGTLVARLMTNGKLTATKAFKSR"
     gene            complement(27517..27714)
                     /locus_tag="PLT31_00285"
     CDS             complement(27517..27714)
                     /locus_tag="PLT31_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93600.1"
                     /translation="MQSEVSLDASAMLLSALSIVSNGGNKYYEKVCENCSTQDPTPIP
                     ENSAYASTFLYTFSEMDYLQL"
     gene            complement(27812..28393)
                     /locus_tag="PLT31_00290"
     CDS             complement(27812..28393)
                     /locus_tag="PLT31_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93601.1"
                     /translation="MGQSLKSKIIIPISIFLLISATATIAATTPYDLIRPTWPLSWDT
                     TAFSTFDTTVTNNKDVLPTEKTPANFKAGELILDTLDQAYLDAINTKISPIRVNQAGY
                     LKKDTERQFYYVGSAEEFEIVDTYGKSLSPKVIGTFSASGASTKSDLTIIAGTNAMTQ
                     NQKRYKVKSTGPSGNILIGQIPMSVPTEKRRLY"
     gene            complement(28606..>29289)
                     /locus_tag="PLT31_00295"
     CDS             complement(28606..>29289)
                     /locus_tag="PLT31_00295"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPW93602.1"
                     /translation="EILLDTVGVADIGDVWKNVKCDEKVVFVIRHAERESGVGKESPL
                     TPNGEIQAMNVGLKLLGSAKFAYAHTDYVRTEQTCKFIALGRGEDTLGLAHDTIRELT
                     SGWYIKDSDLRDTYISTDTNSNAVVSFWAYDSLFADAYYDFASRNQEVIDSFFVKEYA
                     LMDKAKVIISHDEFVVPLIAYVTDRQANFRKRKARWWINYLTGVAIIVNSENQVRYFA
                     VKGLETATE"
BASE COUNT         8849 a         5233 c         5898 g         9299 t
ORIGIN      
        1 attgtgattg gccatacgat tatggcaaaa ggtgctattg ctgaagatgg tacttcttat
       61 gaaggtcagg tgagcaccca tggtcagcct ctcaatgcag ctggtgcttc taccgaagcc
      121 accgtagctc gtttaggcgg taatccacaa gatccattcc aaattttccc agaagtaaaa
      181 aaggcttttg cctctcgttt agacgagctt cgcgcgcaag tcaaagaatg ggaaaagaaa
      241 aagtctactt gggataaaga aaacgccgaa ttatcagcac gtttagattc ttggctctct
      301 ggtcaaggtc cacgtttaaa cctttctgat ttaccaatca aggaaggcgt tgctactcgt
      361 gtttcttctg gctctgttct tgctcattta gcagaaaact ataagaatat tatttgtagt
      421 tccgcagacc tttctaatag tgataatact caggcattct tggatcgtac cggtattttc
      481 cgccctaacg attttaaggg ggctttttta caagtaggcg tagctgaact cacaatgggt
      541 gctattgctt gtggtatcgc tcttcatggc gggctttatc caatttgtgc cacattcttt
      601 gtttttagtg attttatgaa gcctgcgatt cgtatggctg ctttgatggg tttacctgtt
      661 aaatacgtat ttactcatga tagcttcaga gttggcgaag atggcccaac tcatcaacct
      721 atagaacacg aaactcaaat tcgtctttta gaagggttaa ctaaagcgaa cggaaagcct
      781 gagatgcttg tcctaagacc agcagatgct tatgaaactt tagttgcctg ggaaatggct
      841 tttgaaaaca caaatagccc tacagcactt gttttaactc gtcaaaatgt agatgtttta
      901 ccaagcgaag gtgtttctca ttacgaacga gccacaaaag cgaaaagtgg agcttatatc
      961 gttagcgata atacaaaacc taacgcaaaa ccagatttga ttttggtggc caatggctct
     1021 gatgttctct taaatcataa ggcagcagaa atcttaagag cagaaggtct ttctgttcga
     1081 acggtttcta tgattagccc taagctcttt ttaacgcaac caaaatctta tcaaaaccaa
     1141 gtcattgaac catggagacc agtaatggct ctctcaagtg gtttgccttt actattccaa
     1201 tctgttgttg gtggaatggg taaaacaatt ggtcttgaac gttttggagc atctgcacca
     1261 gcacctgttc ttgaaaaaga atttggttat gttccagaag ccgtggcaga aaaagcaagg
     1321 gcatacttag ctgaatataa acaagcttgt agtgattttt tgaaagcaaa tacttagttt
     1381 tttagaacta gattcttttg cctatcattt ctctttgttg tttcgcacat ttatttgctt
     1441 agttataatc tttattggtt aaatgattct ttaaaggtta tttttaagat gtgtgtttta
     1501 tacaaatttt tttctaaaaa tgattttttt gataaaatgt ggtgtgcgtt ttgaaaaata
     1561 aaaagtgatt tttggattca tttcaacgag ggaaaattat gcgtacaata atctttttca
     1621 ctttgctttt tgcaaatact cttatggcac aagaagctcc tgataatatt cgaaatctat
     1681 gcgatactct tcgtattggt gaaacgacag aagaaagaac aatgaagatt agtctggata
     1741 ccatttatga ttttagattc gaaaacgata atcaaataaa ttctcatttc tatcgttcca
     1801 ttgaggatct ttctccgatt agaggaagta aaaattttgc ggatttaact attcctgttt
     1861 ctattgaatc ccaatgctta aaatctaaca atgcctgttc tattgctttt tatgatgcgg
     1921 acgccaaaaa atgggcggca aaagatactt cttgcgtgac tcaaatttat gatattcacg
     1981 aatctctttt tgatgtatac gattcatcca attcttataa ctggattcaa tatcgttttg
     2041 aggattggag tgcgaatgac cctatgaatc aaacggatat tctcttatct gtgaccccag
     2101 aaatgagagc ctcaagttgg cttaccatcg cttatgttgt ggatactgtt gatggaggag
     2161 gttttcgtta ttcgttaggg atgtctagga atgtcgattc tttagaatca gctacgggag
     2221 caatagatct cttagctgcg gcaaaagata aagtgattta tcgtcagacg ttaaaattcg
     2281 tactagcaga agaaaaacta attgtagaag atcctcctat cagtataaag aagtccattg
     2341 aaaatccacg gccatcgttt tctatgcaga ggatggatgg aggcgtttta attcgtggcc
     2401 ctaaagggga agtacctagt ttaagacttt tgaatggaac ctccgtttct ggaaaagttc
     2461 ccgtttctgc tggagtttac tttatttctg ttgaaaaagg gtcttggcat cgtatttttg
     2521 tccaagatta actcctttct ttttcctttt cttttttata gattaagagc cagctttaag
     2581 agctggtttt tttatacatc ccttgcaggg ggcttttctt acagaaaagg ctgcaacgta
     2641 tttgcggagg atgtatggta aatcgctctc ttcttcttgt attctttttc gggctcgcct
     2701 ttgtatttgc aggcgatcct aagctttctc ttgattttgt aaacacagat atcaaagagg
     2761 tggtgcggac gatctctgcg gcctatgata tggcgattct tgtcgatcaa aatatctcgg
     2821 caaacataac ggttcattta gatagcgttg gcctttttga agggctttcg gcgattgcaa
     2881 gtgccaatgg acttgaagta tttcaagaag ggtctttata tcgaattcga aaaaaaatag
     2941 aacgaggtga aaattacttt tcatcgaaag aggggcttgt ttctattgat gtccagaata
     3001 aaggcattca agagtttatt aaagaatatg ctgcgaatac taatttgaac atcttaatcg
     3061 atccaaatat aaaagcaaaa attagtgggc gtttggaggg gttatctcct ttttctgcat
     3121 tagtctcatt aatgcaagca catggctttc gagtactgca aaaagagaat ttttttctta
     3181 ttgaagaaga tcggcattca agagaggagg gggcttcttc tgttttagat cttgcttata
     3241 gaaattcttc tttttttgtc gatttgcaaa gtgcatcctt aaagcaggtt ttaaatgaga
     3301 tggctacgct tgctcatttg aatttagcgg tttatggaga gcttcgtgaa aacgtgcatc
     3361 tcaaagcaga ttctctttcc ttaaaggcgt tattaaaaat gctttttcaa gggagccgtt
     3421 acacttatac atttgattct cttggctcca ttttagtcgg agaacggggg gctccttctg
     3481 cgattgcagg gaactctttg tattacttag agcatattca ttctgaaaaa gctctattgt
     3541 tgctttcaaa agcgtatcaa gatcgagggc ttcgcatttt agaagtgaaa gaacaaaatg
     3601 cattgctgtt gatgggggct tctacagaaa tagaatcagc cacacaattt ttaaaacaaa
     3661 tcgatgtaga aaacctacaa gtgttattag aatgcatcat tgtagagttt aataagggaa
     3721 aacaatttga gtttggatta cgctcaggat cagggaaaaa aacagcagaa ggtgggctag
     3781 gcttaaatag ttttattcgt attacagata aaagtcgctc ttggggaaga gggtttggag
     3841 aaatagggct tctttcagac caattcgatt ttgaattagc ggctcttgaa gaaaataatc
     3901 aagccgagat tttagcaagg ccaagtatta gcaccttaaa tggaaataaa gcaagtatta
     3961 acgtgacgaa tacgacttac tatatggtaa gtcaggtttc tgcagatggg tttccgatta
     4021 cagattatcg ttcttttaat gagggtgtca gtttagaaat tactccatca gtggcacgta
     4081 gcggagaaat cactatagag atcattccag aaattaaaac ttcagcaaga agcactggag
     4141 aagggccaag agatattagt actcgaaact taaaaacaat cgtttcttta aaagaagggg
     4201 aaactttttg tctaggtggc ttgattcgaa aaacatctgc aaaaattcga tcagcaattc
     4261 cctttttagg gagtcttcct tggattggaa aatggtttag ttatcatact aatgaagaat
     4321 atacgatgga actcgctgtt tttattacgc ctcgtttgat tcgtaaaggg atgaacaaga
     4381 ggacgccatg attttagtga aaagagaaaa agaaatggtt caaggcgttt cagaatgttt
     4441 tacgttgacg ctagaacttc aaacgccttt tggtgaagag ccgcatccag agatgatttt
     4501 agatcatatc aaaaaggaat tgcctctttc ttgtgaacga gctcaaaatc gaatttcttt
     4561 tttaccattg atttctttca atagttcgat ggggagaacg catcgttttc tcgtggcttt
     4621 ttcattaaaa gatttttcta agaaccgttc tttttgtgat cgcgttttac ctttaggcat
     4681 tgcttttagt gcctttgcag aagaggagat gataaataag gatggttacg aaaatttaat
     4741 atgtggaggg gttttaggag atcacttttt tttattgatt ttttataatg gcaaattagt
     4801 tcactggatt caggaacata tagagtctaa aacatatgat ctaaacaagc ggattcaccg
     4861 tctccgactt tttattcaag cggatccttt tctttctcgt ttaggagatt gccctatttc
     4921 tattctctct gaagaaaagc attattcttt ttttgaaaaa gccattaacg atcctttgtg
     4981 gaggcgcttt gatttagatc cttctgtaga attaaaagaa cgagcatgga aacgattaca
     5041 gaggcgtttt atttatgctt tcattctttt actgatcttt tttgtaagta cttttttctt
     5101 aaatcagttt cttttttcat ctaaagaaat gattttagca aagcaagcca aagacgagtt
     5161 atatcaagat gcagaaaggc aaaaaatact agcgttccaa aatcaaatac ttcaattgcc
     5221 atttccttta gatttagatc ttctcttaca agaaataggg aatgctgtag ccaaagggac
     5281 aaggctaaag gctctaaaga aaatagcctc agaagaaggg gactattatc tgttaaattt
     5341 ttcagccatt cattttgaag aagcttcacg tttagaaaac aaattgaaaa atagcactgt
     5401 ttttaaggtg aaaaacataa gggtaggccc tgtacaatct actacagacg ggcgagtggc
     5461 ttttcaaatg gatttattct tatgaaattt aatcaacagc aaaaaatcgt gctttggctt
     5521 ttgggtatag gggtgttttt ttctttgggc tcttatttta tcttataccc aacgattcat
     5581 aatatttttg aaaaaagaga attacaaaag aaacaactac ggcagccaat ttcctttact
     5641 tcttgggatt ccattagagg ctcttgggaa cgattggaaa atagttttaa tcattcttta
     5701 ttttctcctg attctttagt gactttggcg ttagaatcgt ctcttggctt aaagcaactt
     5761 aaagtattag caagcggaaa tgaatatcga tttcatatag aaggtaattt tgattctatt
     5821 tattctttta ttcaagcaat agggatcaaa gccccctact gggctttcga acaatttgaa
     5881 cttgtaaaat catcaaagcc caatctgtat tatcttaaaa atgtttgctt aaatcatgtc
     5941 cacataaatt cgaacgaatt aaaagaccat ataattcagg aattagaagt taaaatgaaa
     6001 atcaaaaaaa tgatttcatt tcgagaacgg aaaaaatatt tgaattttaa tccttttgaa
     6061 aatgagagag tcgttcctat taaggtgaca agaaaaagcc aaagctgtcg tttaccttta
     6121 tttcagatta aaggggtcgt tgctcgccgt gttcttcatg tagaacaaga agggagtaaa
     6181 ataatatata ggatggggga ttttattgga gattatcgaa ttcagaacat tgaaacgaaa
     6241 cgtattctat tgatttgcgg tcaagatact atctcaaggt atttatgaaa aatcagaagt
     6301 ctggagccat tttaccttta gtgtgcttac tttttttagc tctatccttc attttttgtg
     6361 ctcttcttca ttccacgcga gtcttgatgc ctcgtgttac ttattatgaa aaatgtttac
     6421 aagaacaata tgacgctgag tctgcgattt tattaagtct ttttcgtgtc cctgcctctc
     6481 aaagtaaaaa gcattatccc atcgtcattt tagatacctt ggggccttgg cttgttttaa
     6541 aatcgaaagc aggtgagcga gaagtaaaag cttttgctgg cttttattct tctgtaaaga
     6601 cgcccccttt ttctcgatta gaattagagc aagcagaaac gaggtgtatg ttattctatc
     6661 aagagcaatg gcgaaaagag tcttatattg atacgttatt tggaacgact cattattata
     6721 agcctcaaat aaaagatttt cctggagtac tatatgtagc agcaggtgat ttaagtatct
     6781 ctttacaagg agagcggttt tctaaagtgg ctttttatgt agaaggggac gttcttatcg
     6841 aaggggaggg aattctagat tctttaagtt tatatgccac aggtgtaatt tctctttctg
     6901 gacaaataaa aatatacgat gcgaatcttt atgcgcgtgg aataataaat atttcagatc
     6961 aaatagaggc tcgaggcgtt ttttttagcg aagatagtgt tgtattacct ttctcaaaaa
     7021 gcgtaggtct tcctttctgg tctaattcta gagacgatat agaaaacaaa gattctactt
     7081 tttatgcggt ggatttagct cacgagaatc ccttacggat tttctcttgg agcgtggaat
     7141 gaacaaaggt ttctctatta tagaaactct gattagcctt attcttataa gtgtgttatc
     7201 tacatcgctc ttgttctttt tagatggcta tagagaaatt caatctctag aacagaaacg
     7261 gtttcaggca ttccgtgaag cgtctgcttg gatggaagcg gaaatagact ctcctccttt
     7321 ttgtttagac tatgcaaaag aaatgccttt agaaacatgg ggtagtatca agttgagtcg
     7381 tacaagaatc actgaaaagg atccgctagt tttaatacaa ataaacgtgt tttctaaaga
     7441 aaataaacgg cttttttctt cattagagag ggtagtagaa tgcgagtaaa aaaaggattt
     7501 actttattag aattgtgtgt aggggtgatt cttacaggaa ttgttttctc tatttctttt
     7561 tcttactttt accagatgca tcgcatttca tctgatttgg ttaaaaagca attagatgac
     7621 ttagaatact ctattcaagt gttgcaagaa gaagaacgac ttcgatctaa atgcattttg
     7681 ttttacacag ataaagaaga gaaaataaaa actctatata aattaaaagc ggcaaatcga
     7741 tgccgctttt aaaaatcata attttttgga atgtattaga cctaagcttc tggttgaact
     7801 tcgatgtctt gagcttgagc ttttctgtcg cggtgaactt cgattaagtt agtgaagtaa
     7861 tgcatgaaac ttaatacgcc aacagagagg aagccaatag catagagagc taagaatggg
     7921 ccgataataa atttctgagc accgatatat tctaacaagc cgaaaatgca gtatccacca
     7981 acgagcaatt caagagaagc aatgattggg aatttttgtg cataagaact taaatgcttt
     8041 tgaccttttt ttggagttct aacaaaagca cctttatgac ctaaaactgc agacatgaca
     8101 gcgcgtgaat tgctgactgc tatacctact ccaacagcca ttaagatagg aagagagagt
     8161 aggcggattt tccatccagt atagccagag caacgttgag ctacaaaata caatgtagaa
     8221 ggagctatgg ctgcaaggaa gataaatgta aaggctatag caaaaacaaa gtttggtact
     8281 tgagcgattg gttcaaacca agcgagaaga gggaatgcac agattgctgt aaaaagcatg
     8341 caaggatgga tggagtagtg agtggtatgc ataaaggcgc cgatctttac acgaagaggc
     8401 acttttgctt tcatcactaa aggaagaact tttttagctg tttgaatgga acctttagcc
     8461 cagcgatatt gctgagcctt aaatgcattg atgtcatttg gaagttcagc aggaacaatg
     8521 acatcaaaaa caaatttcat tttccaacca gcaagctgac tacggtagga aagatccata
     8581 tcttctgtaa gagtatcgcc ttcccagcca ccagcgtcgt caattgcttg tctacgccaa
     8641 ataccagcag taccattgaa gttcatgaat aaattgcccc agctacgagc ggattgttca
     8701 atcacaaagt gaccatcaat accaatagac tgagcaagag ttaaaccaga ttctgtacgg
     8761 tttaagtgcc cccaacgacc ttgaactaga ccgatagttg gatccatcac catataaggc
     8821 actgttttta agaggaagtc tttttctgga acaaagtcgg cgtcaaaaat agcgaggaaa
     8881 tcacctttag cgatggccat gccttctttt aatgcaccag ctttgaaatc tttacggttc
     8941 gttctgtgaa tcaaagtaat gttatagcct ttggctttca tttcattcac tttacgggtg
     9001 gtgacttcta agcattcatc tgtagagtca tctaaaacct gaatctcatg gcgatcacgt
     9061 ggatagtcta tagcacagac agcatcaatt aagcgttcga cacaattggc ttcgttatag
     9121 actggaagtt gtgtggtgac cataggtaga tcatccatgg agtggtctct ataaaagcgt
     9181 aggatgtttt tgcggtcagt gagtcgtact cgacgactat tctttaaaaa gagatatata
     9241 ctgacataac agttaaatcc gtatgcaatc aaaccgatac cggcgattac ataaaccaca
     9301 aacatgagat tcaataaaag ttggttcatt attaaaaagc ctttgcactt tgtatgcttg
     9361 ggagccaaat atagaaaggg attttaaact ttgctacatt tctttataga aaagcattat
     9421 aattttatta tgatttcgac tccaatacaa aaatggctcc acgtaaatag aaatttacct
     9481 gattttgata aggctcttca atctcttttg ggtcatgctt gtgtggatat tttacgtcga
     9541 aatttcccaa atagtcctgt tttttcatta gaaaaggctt tttctattct ttctctctat
     9601 tatcaagaag aattaaaggt gcctttgctt gattttatga gtgaacctct tcaacatgcc
     9661 tctcaaattg ttgatttttg tgaaaaaacg tctacattct cttttctgaa aaaatttcca
     9721 gaagtggatt ttttaaatga aaagcttgat cttcgaggag tcgtctgtcc agggaatgca
     9781 gttcgagcta aacttgtttt agcgggttta ccagcgggga gtactttgca aattgcatta
     9841 gatgagggat acccgataca aaatgtgccc caaacactag ttgcagaagg ttattttatt
     9901 aaaaatcgtg aaaaaaaaga cgattattgg ataataacgg tactcaaaga agaagaacca
     9961 aagtaaatta ttgttgtgga aaatagaata cttgtattag cggatgattt ttttggtgaa
    10021 aacggcgaag ccgcttctca tttttcagag ttattattgt gtttagctcc agacttgtcg
    10081 gtgcaatttg ttcttcttga tccagttttg attcctcttg aaacgctatt acagcgcgct
    10141 ccatctgata ttatagggaa gcaagcgggg cgaatcgttt taggtcttgg tttaagagag
    10201 ttgcggttac accaaaaggc ttctttaatt ttcaagacat ataaggcttt attagatgaa
    10261 ttgctttcta aaacgaaggc tcatctaaat ctagtgactc taccagaaga gtcttttaag
    10321 gaagagattc tgcaagtatc ggagtggaat gagttattaa aatcgatttc tcaccctaga
    10381 atcactgtgc tcgattttgc agatagggca gcacaattta aagaaactca attacaaaaa
    10441 gggaagtttg caaggcgtct atatagtgaa gactctcgtg cgacttcctt atgtcatatg
    10501 ttgctcggct tatttttaca agaacgaata ctaaaagaaa tcaaggagat atcatgagtg
    10561 attttgaacg attttcagca aaaaatgcag caatgagtcg ggcttgggaa atggattccg
    10621 tagaaaaaaa gaaagaaaaa aaaggccatc attcttcaga accagtctca ggaatttgcg
    10681 ataaatgcaa aaaagaagcc atggtaaaat cctatcgttt tcgtcaaacg gatcggacgg
    10741 acggtgccgc aagctttggt tcagtggtaa aacacctctg tccagaatgt gctccaaaag
    10801 ataaacgaaa tgaaaatgcc cctcctcctc caacagatag acaggtaaaa aaccttttgc
    10861 gtggtgcttt acgtaagctt cgttaaaaga ttcttttcat taagttgcat ttacccctgc
    10921 actaaatggg cttttcattg taaatttgct tcggttttca gttacgcgcc ttaatttatg
    10981 ggagaactaa atgccgaagc ctagtttttt tgtttcaata aaaacttttg tatctggttt
    11041 ttcaggagtt tgtacgcctg aattgtataa ggtactaaaa aaagggtata ccaagtctaa
    11101 tttaatgagt gacgcgatgt ctggcgtgat tgtcgggatt ctcgcgctac cacttgcaat
    11161 tgcttttgcg attgcctcgg gcgttggtcc agaaaaaggc ttatatacag caattattgc
    11221 tggtttatta atatcggttc tcggaggaag tcgctttcaa ataggtggtc ctaccggtgc
    11281 cttcattgtg attgtttttg gtattgttcg tgaatatggt tatgatggtc ttgctacagc
    11341 gacattgatg gctggcgtga tgcttgtgat ttttggactc gcgaagttag gtggtattat
    11401 taagttcatc ccctatccag taacggttgg ttttaccgct ggaatcgcga ttattattgc
    11461 tttaggccag gttccaaatt tcttaggtct tacttttcca gaaggcgtca aagaaccagt
    11521 agaagcctgg ggaaaaataa aactatatgc ctcaagtatt acttctttta attgggtttc
    11581 ctttttaata ggtggtttgg ctctcgttat ttgtctttat tggccaaaac taacgaataa
    11641 agtaccagga tctttaattg caattcttgt aacgacgatt cttgtgaaaa cattgggctt
    11701 agatgaaaaa tttggagtaa ttactattgg ggcaaaaaat catattccaa caggttttcc
    11761 tattccagaa ttgcctaata ttagtataga aatgatgcgt aaggtttttc aacctgcttt
    11821 aactattgcc attcttggcg ctatagaatc tcttttgagt gctgtcgttg cggatggaat
    11881 gactggaagc aaacatcgtt ctaatacaga gctagttggg caaggtattg ctaatatttt
    11941 cagcccaatt ttcggtggta ttcctgcgac tggtgcgatt gctagaacag caacgaatat
    12001 tcgtaatggg gcttttagtc cgatttctgg tgtagttcat gccctcgttt tacttttaat
    12061 tatgctcatc tttggtaagt atgcagaaat gattccgatg gccgctcttg ctgcgattct
    12121 tttccaagtg gctttcaata tgtcgggcta tagaagcttt attaagctat ttaaggctcc
    12181 taagagtgat gtgactgttc ttgttatgac ttttgcttta accgtcttat ttgatcttac
    12241 cgtcgctatt gaagtgggcg ttttacttgc agccatattc tttatacgta gaatgtcaga
    12301 agttgcccaa gtggattttg taacgcaagc cctaaaagaa gatgatgaag aaatttctga
    12361 ttcttataat cttggttcac gtcaagtgcc aaaaggcgtg gttgtctatg aaattgctgg
    12421 ctctatattt tttggtgtag tagataagtt taaggaaacg ttagaacaag tagcggataa
    12481 tccaaaaatt cttattttga gaatgcgtga agtacattct attgatgcta ctggcattca
    12541 aatgattgaa gaacttttga ttcgctctaa aaagcagaat actcaattac ttctttctgg
    12601 cgtacatact caacctgtag tagccctcac tcgtgctggg gtaatgtcca tgatagggga
    12661 agaaaatgtg cttgctaata tcgatgctgc cctaaataga gctagagaaa ttttaggaat
    12721 agaaatgatc gagccttcag aaatggaaga gtccccgagt gtttcttggg aaaagaatct
    12781 cgataaacca tggcttccta atcatgcgaa cggttctgtt gctgaatcca caagcgaagt
    12841 cattgcagaa aaagtagcag aagaaacaga atccaaagaa actgagtcca aagaataata
    12901 atttggctta tccatggaat tcataaaagt cggtgaatac cgtctttttt aatgcattga
    12961 atacattcat tgaaatgagt atattgtttt aagaatatta tttagtgcat accattattg
    13021 aataaggtaa ggatatgtat ttcacgctta tagttctctt gttagccatg gtctttttta
    13081 ttcagggcaa aattcgttct gatatagttg ctctttcggc gacggtgctt ttgatgcttt
    13141 caggtattct aagtgtacca gaggctcttt ctgggttttc tagttctatc gtgatcatga
    13201 tgttaggctt atttgttgtg ggtggcggcg tttttagaac aggtctagca agatggttag
    13261 gtagtcgtat agtgagtctt gctggaacga atgaaacaag ccttttatta gtcatatttt
    13321 tggtcaccgc ttttatgggc tgttttgtga gcaatacagg gactgtggct ttgatgcttc
    13381 ctattgttgt cagtttagcg gctaatgcta aaataggagt agaacgctta ctaatgcctt
    13441 tagcttttgc aagcagtatt ggtggtatgt ttactttgat aggaacgccg cccaatatgg
    13501 tgattcatga aactctactc aaagctggaa aaaatggcct tctattcttc tcatttgcac
    13561 cagtgggagc tgtggctttg gtagtgagtc ttgctatgct tatcccatta tccaaattat
    13621 taaaagggaa gaacaaaaat aaagacaaaa ataaagccag tcaaaaatct ctgaatcagt
    13681 tagcgaatga atatcaatta gtcgatagca tataccgagt acgcgttcct gcaaattcac
    13741 ccattgtaca aaaaaaactt attgatctca atcttccttt gcaatacaac ttaattgtga
    13801 cagagattcg tcgtcactct cgttcctcta gattattaat gaaggaagtg caacaagaaa
    13861 ttgctggcgc aggtacggta ttttttgcag gcgataccat tcatttgtta ggggcttcaa
    13921 aagatgtcgc tttttttgca aaagagaata atctacaatt gatagaatca gaagaaggca
    13981 acaataaatc attccagttt ttcaaagttg gaatagcaga agtcttgtta cttccggatt
    14041 cttttttaga aaataaaact gttttagatg ctcaatttag aacgcgctat ggagtcaata
    14101 ttctttgcgt gcagcgcaag ggtaattata ttgtaaagga tttaaaaagc cttccttttt
    14161 tatctggaga tactttatta gttcagggca gttgggatga tattcgtaaa ctttctgaag
    14221 aaattaataa ttgggttgtt ttagggcggc cattagaaga agcagcaaaa gaaactttag
    14281 atcataagat gcctctcgct gcttttatta tggttgctat ggtggttctg atggtagcca
    14341 atatcgttcc tgctgtcaca gcggttcttc tggccgctat tttaatggtt ctttctgggt
    14401 gcttccgtaa tgtggaagat gcttataaat ccatcaattg ggaaagtatt gttttaattg
    14461 ccgccatgtt accgatgtcg attgcgttag aaaaaacagg gctttctgcc tcgttatcaa
    14521 gtgccctagt ttcttctgtg ggctcttatg gaaactatgc tttattggcg ggaatttact
    14581 tcaccacttc tttaatcacc atgtttatca gtaatactgc taccgctgtt ttgttagctc
    14641 caattgcgtt aacgtcagca gcagggcttg gggtcagtcc ttatccattt ttatttgcgg
    14701 tgagtgtggg tgcgagtatg tgttttgcct ctccattttc tacgccaccg aatgctcttg
    14761 tgatgaatcc aggtcgttat acctttatgg attatattcg tgtaggtctt cctttgcaaa
    14821 tagtcatggg aatcgtaatg gtatttgctt taccaatcat tttcccattt aacccttaat
    14881 ttttatttgc aagtatatgc aaaatacgtt ctctcccaat actagattct aaatcggaaa
    14941 aaacttcaga agcaaaattt aagagcagaa tttcttctgc tttttttgca tcgattccgc
    15001 gagattgcaa gtagtataaa ctttctgcat ctaattctcc acaagtattg ccgtgcgtgc
    15061 attccacatc atcatggtaa attttaagaa ctggttttac cgaaacttta gcctcttctg
    15121 aaagaagaat actgttgatt agctgggctg agttgacctc agtacaatct ttctctacat
    15181 gaacagcacc atcataactc acatggcttt taccgtttag aatttgccgt acgaattgat
    15241 tgtttttcgt atgtggagca ttatgatgaa tggtcaagtg gtaatgtgct tcggtttcgc
    15301 ccttcaattg atttagcaaa cgaaagtcta ctgtagaatc ttcgccattt aaaaagagat
    15361 caaaactttg cctataacat ccagtgtttt cttgaaggcc taatattttg attgatgctt
    15421 ttgcagcggc aacgattcga acatgtctaa attgaagact atgattttgt agcggattac
    15481 aaaagaacaa ttctagcttc gcattttctt ctacaaaaaa atcaagacgc tctgctgtaa
    15541 aagcatgttc atttttatta tctaaaattt caagagaaac gtcggcgcct ttttgaatat
    15601 gaaatagcgt atggctaaaa tcatctctag cttttagaac tcctgtttct tgggcattcg
    15661 catcaattgt ttttaagaag gtattacgag tatgaatgag agggattaag gcggcaatat
    15721 ttttttccgt atcaattccc aaatcaaatt ctttagaaat actttcagaa aaattctctt
    15781 cgggggagat gacgttaatc ttttgaaaag cagaattggg aaagaagatc caatcatcat
    15841 gacgagcttt tggtaaacca atgctttcaa atcgagaata agctttttgt tgaatattca
    15901 ttaggcttcc tctatccagt cataaccttg ttcttctaat ttaagcgcaa gttctggacc
    15961 accgcttaga atgatttttc cttgtcgtaa aacatgcaca aaatgaggtt ttacatagtc
    16021 taaaaggcgt tgataatggg tcactaaaat gacggatttt tctggagtca tcatttcgtt
    16081 aatcccgcga gcgacaatac gtaaagcatc aatatctaaa ccagagtctg tttcatcaag
    16141 gcaagctact tttgggtcaa ggattgccat ctgtaaaatt tcatttcgtt ttttctctcc
    16201 accagagaaa ccttcattca ccccgcgact aatgtagcga tcatccatct ccaatagtga
    16261 ggtgtatttt tccatgagtt gattgaactc agagtctttt attttttcta atcctaaata
    16321 agctctttta gaatcaagag ccattcttaa aaaatccacg ttttttacgc ctggaatttc
    16381 aatagggtat tgcatgctaa tgaatagccc tgcatttgct ctattattaa tatccatgcc
    16441 taaaatatct tctccgttta agataaccga tccagaatcg atttgatacg aaggatgccc
    16501 gctgatggct ttaaacaagg agcttttccc agagccatta ggccccataa tggcatgaat
    16561 ttctcctggt tgaaccgtta tgttcacccc ttttagaatg gatgtaccgt ctttaagact
    16621 tgttttaaga ttttgaattg ataacataag gggatctccg tagttttatc cttcttgaat
    16681 tttatgatga aactcaagta aaagggttcg gttttctaaa atttgatgaa ataaatcact
    16741 actattaata tggccaagat tgctttctct taaaagtttt tgctgctttt ttgaaaacgc
    16801 attgatctct aaacgtaaca tagtagattc ttcgttttct ttttgcttga gctcattaca
    16861 aaaacgatca cattggcgct tgaataaaat taatagggtc tcaagaaact ctttttgagc
    16921 gttggctggc gaccagattt cttctggaag cccttctaga atttctggaa gaggaggatt
    16981 tgctatcgag tgaagtattt taagatcaat tgtttggtgt tctgcatata aagctaaacc
    17041 atcaccgagc aattcttcta aaagagaaga ctctaaaaga ggaagatttt gagcaataaa
    17101 atcaaggcta aaaaagcggc ttgctggttt ccataaatca ttatttctta aaatcaagtt
    17161 cacaaatcga agttcaacgc cagaaagtat attccaaggc acctgaggtg tattatcaat
    17221 ggctggcagt tcttctacag ctttcttttt tactgcaaca gggcgaacat gagaaagcga
    17281 acgagacgtg ttaaatcttt cagaaagtag attcagatat tgttctttta attctacatc
    17341 ttggattgct gcaatgagcg atttcgcgtg atttacaaag actgcttttt cttctggact
    17401 ttgagtttct ttttcgatag aaagatagtg aagccagtct aaggcttgtt taatttcatt
    17461 gagaaacgca ctcgctccat tttctcgaac aaaattatca gggtctattt tttctccatt
    17521 gggccttgaa agagagaaaa tacgaggagc aatattatga ggtaaaagaa tttctaaact
    17581 tctcatcgtg gcttttttac cagcggtatc accatcaaag actaagaaag cggttttagc
    17641 atatcggccg agaatcttag catgcatttc tgtaagagct gttccagaaa ctgcagctac
    17701 attttttatt cctgcttggt aaagagaaat taaatcaaaa taaccttcta cgagaataac
    17761 ggcatcttct tttgcaataa aattctttgc atgatttaac ccaaagagaa tatcactttt
    17821 ggaatacaaa atggtttctg ggctattcat gtatttggga gctctaggta tatccttcat
    17881 aatgcgcccg ccaaaagcca cgaccgttcc agataaatta tgaataggga tcatcaagcg
    17941 accacgaaat ttatcagtga tgcctccatt ttctttttct atcgccaggc cagcttttac
    18001 tacttgccta ggtgaaaaac cttctttagc tgcaagtgaa ataaaatctg agggattttc
    18061 tggcgcatag ccaattcgaa attcttggcg agtttcttct gtaatgcctc ttttagcaag
    18121 gtattctaag gcaggagcat ttaatgataa ttgattttca aaccaagtgg ctgcaagctc
    18181 gttcaaatga cgaacaaact ctttttcttc tgtgatttct gctttttcgg gttctccaag
    18241 aacgggtagt gaaaaaccac tttcttgagc gacccattct acagccgcct taaaatcaag
    18301 cttttcatgc tccatcacaa atttgaaaac atctccacca gcgccacagg caaagcattt
    18361 ataaatccct agttgaggat tcacattcat agaaggagaa cggtcatcat gaaaagggca
    18421 tttgccaatg aaacgcccca cgcctgattt ttttaaaggc acaaagcgct caataattag
    18481 agcaatatca gcgtgtgctt taagttgttg aatcaggtcg tcgttgtaaa aagccataag
    18541 ttaaaagata attaaaccgt gcttacatga ctccaagagt ttgccaacga ccctttctcc
    18601 agcgaatata attaacaata gaacgataaa attcatcgca agccattcca agccaaaggc
    18661 ctataatccc caaatctaaa acaaaggcca gaaataaagc agagccaaca cctaaagtcc
    18721 acatcatacc agaaccaacg attgcaggga acgaagaatc gccagctgct cgtagagagc
    18781 ttgtaatcac aatattcgcg gctttaaaag gctgaagaac gacatcgcac cagaggcacg
    18841 ccgctccaat tcgaatgact tctggattat cggtaaaaaa agtcattaaa tgagcaccaa
    18901 aaagcgccac agataaagca atcaaaaagg aactaatgca gcttataatt aaactttgat
    18961 gaaggcgacg atttgcttta ttgtagtcgt gtgcacccac gagatgagct acaagaattt
    19021 ggtttccact ccctaaaccg acgcttaaaa tcacagcaag agcagctaaa ttcgcactaa
    19081 aaattcgaga ggtcatcgca atggttccta agcttacgac catggcggta ataaaaatct
    19141 ggaataattg aaaactaaaa ggttctgcag ccgcaggaag gccaatacga atccaatcgg
    19201 gcaaaataac acggattcgc ttaaaatcac ttctttgatt cttgtggtgt attttagttt
    19261 taagaataaa accaagaaaa attgctgaaa ttaaccatga aatagcagtg gctaaagcga
    19321 caccatgaac tcccaattga ggtaagccaa accagccttc taagaagact acatttagaa
    19381 aagcattact cgcaatagtg attatattac caatcaaatt ccagatggtt aaaccatgag
    19441 ttgcaattaa ggcagtataa gttgtctgta aagctctaaa agcaaaacca aaggctgtga
    19501 caaataaaaa ttgttcagcg taggcggcag gggtgggctc taatccaatc catcttgaaa
    19561 tggaagagga aaaaggaaga ataagcaaag tcatgctaat gccaagtaaa aaactcccaa
    19621 tgataatcat tgtttgagta acacgagcat gtctgctata gttgcctcca aggtactgtg
    19681 aggcgatact tgctcctgct tgagagaaag cttgtattgc catgaataaa gctcccaaaa
    19741 taggtagaac agcgccaacg cctgctgcgg ctacttcaga ggtgcgtgaa agaaaccaac
    19801 tgtccataat gggctgaaac atgccaacac caaatgtcag tatcaatggc caggataaac
    19861 taaagagaga gcgatccttc acttgcataa cgttaaattc tagaaatgag tagggtttct
    19921 tggaatgggg tttaaaaatt aagaggttcc tcttgtaaac aaaaaaagaa atccactttt
    19981 aaagagctta attatttgga tagcagtttt tctcttagtt caagacgtgc ggctcttcta
    20041 atttcagcat tattataacg tctcttttca tcttctgttt cggggataat cgcaggaact
    20101 tctttgggtc tacctttttc atcgatggcg acaaatgtca tataagctct gcaaatatct
    20161 tcatttggtt ttcctggaag ccatgccgtg actttcaagc ctatttccat tgaggttctg
    20221 aaagctcggt ttacggatgc atgaatagtg agcattgttc ctactggagc gggtcttaaa
    20281 aaatgaatcc cgtcgacaga aacggtagtt actttagatt cacaatgacg aaaagcagcc
    20341 atgcatgccg ttttgtccat taaagagact aaatggccac caaaaacaaa accataggcg
    20401 ttcacgtcac ttggatggac gatgtcatta gtctcgatga tggattcttg aactgttttt
    20461 gctttcattt tcataggact aatcttagaa aaaataaatg gataggggga gagaataaaa
    20521 aaagaataga atgatannnn nnnnnnataa ttcattaaaa tctctcaaat aaatgaatga
    20581 ttttttaata gtgaatctaa gttaaaaaat aataaaacgc tgtttttgaa gtaatttaag
    20641 cattttaagc ttttttatgg gctttcaaaa aaaacatttt tatatgcaaa aagaagtatt
    20701 aattaaaaag atttttcaaa ataatccgaa aggattatat gagatttgta agaataaatt
    20761 attttattag ctgttattta taatacttcg tttatagaga attataaata actgtgtgat
    20821 gataagtaat aaaccctctt tttaagaatg gcaaattgac ttttgtatag accctcttaa
    20881 aaagaaatat cattccgagg atgatcgagt aaattcggaa tgaagttttt ctggggggag
    20941 ctacgcaaag caatttgcaa tagagctcgt cttcccgtcc ttagttcatt ctaagggcgg
    21001 gatttttttg acacaaagat gccttttaac acctctgtaa aaaaaataat tggtatgaaa
    21061 cacaaaaaaa gtttataaaa tcaaaaaaat aggctttttt tatttgaaaa gaaacatttt
    21121 taattgaagc ataaaaaaag gtttgctgaa atattcaaca aacctttata aatacaatcg
    21181 ccaacttttt aattatagaa caagcgaagc ttttctgtaa tttggtctaa atcgaccatc
    21241 tcaaagttct tttctgagcg aagtttccat tcgacttggc cttgttcaag gccttttttg
    21301 ccaacaccaa ttctcactgg aataccccag agatcagcat ctttgaactt aacgccaggt
    21361 ctttcttctc ggtcatctag taacacttca aagccaagag aggtgagttc tttttcgagt
    21421 ttatctgcgg cttcgatgac attggcttct ttaccaatag agacaatttc tacttggaaa
    21481 ggggcaatgc tttttggcca aataggacca aattcatcat gagagttttc gacaacactg
    21541 gccattaaac gaccaactcc aatgccatag catcccatca cagcaggttc gctttgccct
    21601 tcaggattca agaaggtggc tttcatgctt tcagaaaatt tagtaccgag cttgaaaata
    21661 ttaccaagtt caatgccacg agtttctgtt agcatctcat cgcaattagg gcaatgacaa
    21721 agagcacttg cttcggcaag gtctaatact tcataatttg aaaaatcgcg tttaggattg
    21781 caatgcttga aatggaagtc tttctcgttt gctccaacga ctagatcgtt agcgttttct
    21841 agagcgagat caattaggac tctagtattt tttgctccaa tagggcttgc aaaaccaggc
    21901 tccattcctg aggctttgat caaggattca tctgcaggaa tcaattcttt cgtttttaaa
    21961 atattacgaa gtttaatctc tgaaacttca agatgacctg gaactaaaac agtcacgagt
    22021 ttcccttcga tatcaaagaa gacgcacttg gcggtcgctt ctggagagac ttgtaaaaag
    22081 gaagagactt cttcaatact ttctttattt ggagtggcaa ccttttctaa cgccgcttct
    22141 gcgttatttg tataaggatg tctttggaat ttggcaattt cacgattagc ttgataaccg
    22201 catttcttac agagaatgag ataatcttct ccactaggtg tatctagcat aaattcatga
    22261 gctaatttac cacccataat accagtatcg gattgcacaa caactggctc aatccctaca
    22321 cggcggaaaa tgcgaaggta ggcttggtac tgttcttcgt aataggcatc tagatctgct
    22381 tgtgtggcat gaaaactata agcatctttc attaaaaatt cgcggacgcg aatcaaaccg
    22441 ccacgagctc ttgcttcatc tctatacttt gttttgaatt gataaagcat aaaaggaagt
    22501 tgcttgtagc ttgataaaac atagcggcta gcatcggtaa cggcttcttc atgagtcatc
    22561 gccaaaacca tgttatgatt attacgatct ttgaatcgta atagttcgtc tccaatggca
    22621 ctataacgtc cagattcact ccaaagttcg gctgtttgta caattggaag atccacttca
    22681 aggcctccca ctcgattcat ttcttctcga ataatatttt caattttttt catcacacga
    22741 tgcccaatag gcaacatgct gtatattcca gtagaaaggg gtttaatata gccaccacgc
    22801 attaaaaaga tgtggcttgc cattgtagcg tcacttggcg tttcacgaag agtagtataa
    22861 aaatagcgag aaagtttcat aaaggttctg tttgttaatt ttggaaaaaa ttagtaaaaa
    22921 agaaggaaat atatgaatta tgtctaacta agaagtcgct tttttgctaa tttacgagcg
    22981 attctaaaga acctgaggag gcttctcaat gaaattaaaa tgcttaatat tattaatgct
    23041 agtgttaact atgctttcag cttgtggaac caaagaaact ggaaattcca caatttcatc
    23101 aaattcaaca gatgaacaaa aattttctta tatgctaggt gtacaatttg gtcgccaagc
    23161 atttcgttta gtgcctcaac aaatgggtca tgaattagaa gaagattata cagttcaagg
    23221 agctcttgat gcggctcttg catatgatga taccactaag gcaattcaaa ttactaatga
    23281 tacgttgaga gctgtcagtg aacgcttttc aaatgtggct agggaacgtt tagaaaaggc
    23341 tcgtcctgat tcagctacaa ttgctaatag tccagatcct agacaattgc gtcatatatc
    23401 cgattcctta ttgaaattat tacctgtgga acctgcaaaa ccaggaacag ggaaagtggt
    23461 gaaggtatca gactcgtctt ctgacttaga aaaattttca tacatgcttg gtacagagtt
    23521 tggtactcaa tttggatcca tcggttctca attaaaagtg actctttctc cagatgcatt
    23581 taaagtaggt ttgttagatg gggcaaaagc ttatcgcgat tcttcttatc aattccaact
    23641 tccagaagat tctcttcgcg ctgttggccg tcgcttctca gaaaagggta gagctgctca
    23701 tgaagaagct caaaaaaaga tgatggaaga agaagctaaa ttaaaagcga aagccgctgc
    23761 ttatgtcgga gacactcttc ctgatggcac accagcaaaa attaatttca caataaaaat
    23821 gagtgccctt aattcaaaga ttgaatcttt tgctccttat gcaggtaaac ctctttttgt
    23881 attttactac tccactactt gcggacattg tcgtaatact gctcccaaag tggaagaatt
    23941 tgctggaaaa tataaaggcg aaggtttaac tactgtcgct attaccacag gcggaacacc
    24001 aaaaagaaat attcttcgtt ttagtgatga atttaaaatt caaaacatgg aaatcttatc
    24061 tgatgacgaa cgtctttttg gtcaattata tggtgatggt tatgtgccaa aattttactt
    24121 agtaaatcct gatggaactt ataagagata cgcttccttt gaagataact tcaatgaaat
    24181 tgaagccgat gtgaaggcat tactgaaaaa atgagttcta taattgcgga atacgatgtt
    24241 gtcgtgatag gcggtgggca tgcgggtata gaggcatgcc atgctgcttg gaagcttggt
    24301 gtaaaaaccg ctttactcac aatgagtatt gatgctattg gtcgaatgtc ttgtaaccca
    24361 gccgttggtg gaatctcaaa aggacaaatt gttcgagata tagacgccct tggtggattg
    24421 atggggatcc tgaccgataa agcaggtata cagttccgca tgttaaacaa aagtaagggg
    24481 cctgccgttt gggggcctcg tgctcaatgt gatatgaatt attatagtga gctcgcaaga
    24541 gatactctca cggcattacc tggcctttcc ctgattgaag gggaattggc ttctttctca
    24601 aggttaccag atcaacgctt tgagctgatt ttactagatg gttcaagata tatttcaaaa
    24661 acatttgtta ttacaactgg tacttttcta gcatctaaaa tgtttactgg taacgataca
    24721 agtgtcggtg gtcgagtcgg ggaagcaagt tctgataagc tttcagaaaa tcttatctct
    24781 gctgggattg cattacgtcg cttaaaaaca ggaacgcctt ctcgcttgga tccagattct
    24841 ttggattttg atgagtgcga agtgcagcat ggagatgaag acccttggtc ttttagcgat
    24901 cgagaacaag ttcctctaaa taataacggc gtttgttgga tcacgcgaac gaatttgaaa
    24961 acacatgaaa ttttgcgctc tgggtttaag gatagcccta tgttttcagg gcgtattcat
    25021 ggaaaggggc ctcgttactg ccctagtatt gaagataaga ttaatcgttt tggtgacaaa
    25081 gatggacacc agcttttttt agaacctgaa actcgagata ttggtcgagt ctatgtgaat
    25141 ggttttagct caagtctacc agcggatatt cagcttgctg ctttgcatac gatgcctggt
    25201 cttacacgag ctaaggtttt acaaattggt tatgctgtag aatatgattc tgtagatgcc
    25261 acgcagttaa accccacttt tgaatgccgt cattatccag ggctttattt tgcgggtcaa
    25321 gtttgtggca ctagtggcta tgaagaggcg gctggccagg gtttaatggc tgggattaat
    25381 gcggcactga aagtgaaagg cgaagaacct tttattctag gtcgttctga aagttacatc
    25441 ggtgtgatgg tcgatgattt agtgaatatt ttattagatg agccgtatcg aatgttcaca
    25501 agccgtgcgg aataccgtct atttttaaga tcagataatg cagaagttcg tttaaaagcc
    25561 aaagcgcgtg aaataggcat gatttcagat cgtgattggc aagattggat tacgcgcgaa
    25621 acaatcatgc gagatgttca tgacttttta tctaaagaat caggaacgcc aacacaagtg
    25681 aaccctattc ttgaacccag tggccaggct cttgtttcgg atcgtgttcg ttggattcaa
    25741 gtgttacgtc gccctggaat tgatccagaa gcctttttta aggtagcgtg cccagataaa
    25801 aaaatgactc gccgagacca atggtttttg tactcagaag aaatctatgc aggctttttt
    25861 gaacgtcaat taagagaaat agaacatcaa aagaaaatgg agaaaattcg actgtctcca
    25921 gatattgatt acatgtctat ttctgcattg agtattgaag caaggcagaa attaatggct
    25981 caaaaacctt tgacgatggg tcaagtcagt catattcctg gtgtacgtca agcagatatc
    26041 actgtattag ctcattggtt tgaaacaagc aaatagtcta aggttatatt attatttgct
    26101 tccatttgaa cgtaaatgcc atagaattgt ttgataataa atgaatattt agaactctct
    26161 aagatcaaaa cctttaaata ctttgcattt ttgctatatt gcgagccgtt ctgttattta
    26221 cataccccga ggttctcatg tccgatactg aaaaaaataa acaagaaagc aagcctcaag
    26281 aaactaaatc ccaagaaaaa aaagctcctg ccaagaaaaa acattctaat gcaggtcgtt
    26341 catcgaaagc gaaccatcct tttaagaagg gagcaccttc taacaaaaag cctgtgactt
    26401 ttgctgattc ccttgaagat cgttttcctg ctttagctgc gaaattgaaa aaacacgttg
    26461 aagatggtat taaacgtaag attaaagacg ctcagtctga tccaaaagtt cagaaagcaa
    26521 gtgccaagta cggagcaaac taattttttg tgtcttagtt cattattaaa gaagcccgcg
    26581 aatagcgggt ttttctttag agtctgcttc aaaagcagac tttttttgta aacacatttt
    26641 gaaaaaaata tttgatttta aataaaaaac tggtaaacta tgcagctacc agttcaaact
    26701 tggttttatt caatgaattg tttatcggct cttaaatgct tttgtagcgg taagttttcc
    26761 gttagtcata agccttgcta ctaatgtccc acggtgagcg agaggagcaa gatttatttc
    26821 agtagaagac ccttcaaatg ttttgcttaa aagctcattg cctaaaagat caaaaatctt
    26881 cactgttttt gaaccattcg cttctgcttc cacgaagagt ctattgctgt tgacttgtat
    26941 tagagcttta cttgcaatag gcatataaaa gatgcctact cgatcatttg tagtatcaaa
    27001 aggcccatat ccccataaca aacttgcctt gctacggata acgatgtagg gggcttttgg
    27061 ggcgggcgtt agttcatctt tgtcccaact aggcgctcca gcgtagtaga taggttcttt
    27121 atcagtaatg gaatgttctg taagactcca gctttggctt atgtctacct ctgtagggat
    27181 attgtacgtt aaacatttgt tttctatttt tgtacctgaa gaggctacta tatctactct
    27241 aatgccagaa cccaaagaca gggaatcaat tacgataggc aaagcccagg tatatgtttt
    27301 attctctttg ttgtaagact catcgacttt ttcggggaga aggttctctt gaatgagttt
    27361 gtcattacta caagctttat tgaatccagc aagatcgact gtttggcaga tatctaacga
    27421 aaacatgata ttgcattgat ctatgtcttc ttcagaggcc tcaaaataga agtaggccac
    27481 aacactatct agtgtttcaa aagacgtatt ttgaatctat aattgcaagt agtccatttc
    27541 agaaaaagta taaaggaacg ttgaggcgta agcgctgttt tctggaatcg gtgtagggtc
    27601 ttgtgtacta cagttttcgc acaccttttc gtagtattta ttacctccat tgctaacaat
    27661 actaagagca gataaaagca ttgcagatgc atcaagtgag acttctgatt gcatgtattc
    27721 catccaactc atgctgcttg gaacaattga attttcattg tcaggagtgg tgcctgcata
    27781 aagagctcca acgggtgtta catacttata ttcaataaag gcggcgtttc tcagtaggga
    27841 cggacattgg aatttgccct atgagaatat ttcctgaagg acccgtggat ttgactttat
    27901 agcgtttttg attctgcgtc attgcatttg ttcctgcaat aatagtcaag tcggattttg
    27961 tacttgctcc gcttgcagaa aaagtgccta tgactttagg gctaagcgat tttccgtacg
    28021 tgtctacgat ttcaaattct tcggctgaac caacatagta gaactgacgt tcggtatctt
    28081 ttttgagata accagcctgg ttcactcgaa tgggagatat ttttgtattg attgcatcaa
    28141 gataagcttg gtctaatgtg tctagaatca attcgcctgc tttaaagttt gcaggtgttt
    28201 tttcagtagg taaaacgtct ttattatttg ttaccgtagt atcaaatgtt gagaatgcag
    28261 tagtatccca gcttaagggc caagtggggc gaatcaagtc gtagggggtt gttgccgcta
    28321 ttgttgccgt agcagaaatt aaaagaaaaa tagaaattgg gattattatt tttgatttta
    28381 gactttgacc cataaacaac tcctgtgaag agtatataat atagttttaa ttttttttcc
    28441 attgaattaa tttatcataa aaaaatatct gtaacagaga acggcgttga tttcgattgc
    28501 ttaaaatcac aaaaaataat gttaaaatca cattcaaata aaaaatccta gaaacgttgt
    28561 tctaggactt ctttgataat cacaggtcag atttttattt agccactatt ctgtcgctgt
    28621 ttcaagacct ttgactgcaa aatagcgaac ttgattttcg ctattcacga taatagctac
    28681 acctgttaag taattaatcc accatctagc cttacgtttg cggaagttgg cctgtctgtc
    28741 tgtgacataa gcgatgagtg gcacgacaaa ttcatcatga ctaatgatga cttttgcctt
    28801 gtccataaga gcatactctt tgacaaagaa ggaatcaatg acttcttggt tgcgcgaagc
    28861 aaagtcgtag tacgcatctg caaatagact gtcgtaagcc cagaaggata ctacagcgtt
    28921 ggaattggta tctgtggaaa tataggtgtc tcgtaaatca gagtctttaa tgtaccagcc
    28981 gctagtgagt tcacgaatag tatcatgagc aagaccgaga gtgtcttctc ctcgaccaag
    29041 agcaatgaat ttgcaggttt gttccgtacg tacatagtct gtatgtgcat aggcaaactt
    29101 ggcgctaccg agcaatttga gccccacatt catcgcttga atttcaccat taggggttaa
    29161 tggcgattct tttccgacac cgctctcgcg ttccgcatgg cgaataacga acaccacttt
    29221 ttcatcacac ttcacatttt tccaaacatc accaatatca gcaacaccca ccgtatcaag
    29281 aagaatctc
//
LOCUS       DAOOBB010000003        28857 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_2128_length_28857_cov_5.231373, whole genome shotgun sequence.
ACCESSION   DAOOBB010000003 DAOOBB010000000
VERSION     DAOOBB010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 28857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28857
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2128_length_28857_cov_5.231373"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..701)
                     /gene="aroC"
                     /locus_tag="PLT31_00300"
     CDS             complement(15..701)
                     /gene="aroC"
                     /locus_tag="PLT31_00300"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPW93603.1"
                     /translation="MSSIFGKEFTVSTWGESHGKAIGAVIDGCPAGVPLCAEDIQKFL
                     DRRRPGQNAMVTPRQEADRVDILSGVFDGKTTGTPISLIIFNEDQRSHDYSDMTQWYR
                     PGHADLTYDLKYGFRDYRGGGRSSARETAARVAAGAIARKILETRYGTEIIAWVSSVG
                     AEESHVDLNKVTYDAIEASPVRCPDPEASARFEKVISDVKDNHDSIGAVVSLLVRRHQ
                     HPLVNPFLIE"
     gene            808..1392
                     /locus_tag="PLT31_00305"
     CDS             808..1392
                     /locus_tag="PLT31_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93604.1"
                     /translation="MQKKAFLFIILTLLLVTEVFSQKLDQFAGISFGLNRQEVIEEIM
                     KLGYDPYGQVGSASDRVIIPVYMLAELPVQVDFLFNKNEKFYAFEIRTGRMEQMRLNK
                     VFEAVAYMSDHFTVKYGKPSKVPSLKEQDIKENVHNIYQEWFGVKFLDIYTAIIKKDG
                     RFYTIGSVTHRTLAKEKNSEGKKNLSSKERVPAF"
     gene            complement(1411..1887)
                     /locus_tag="PLT31_00310"
     CDS             complement(1411..1887)
                     /locus_tag="PLT31_00310"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HPW93605.1"
                     /translation="MRETKKIWLSTIFIMFYGILLGNMVFAEDTTNSLNPMMDKPAPG
                     SKADTLTREDARMAYLIYNLLDENKRIKGANLKRGAELFYMNCRACHGDDGRRVNFTP
                     NKKTATYLGTTARNEMPTFWYLMNFGDGGRGMLPYIDEIPLEDMIDIAGFAQTLPE"
     gene            1949..2986
                     /locus_tag="PLT31_00315"
     CDS             1949..2986
                     /locus_tag="PLT31_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HPW93606.1"
                     /translation="MTLNFSISSAATIIPRDSSSLEFYGRWLIEKDQARTSAPGASVQ
                     FSFEGTSIYAELEGESYWTVEIDGKEYPSILTGSRQKYALAENLPIEKHQVTLSKKSE
                     TPTTEVILYSLSTNKEGSFAKPRRRPDRRIEFIGDSFTVGYGNEAKNSTDGDVFEKTN
                     ATKSFAYLLGQSLRADIQINAFSGRGLVQNYANHTPEWSALKLYEYTIPGIAEKQPNT
                     PLWDYSKWHPHVIVIFLGINDFQGDFDHTKPSTFDSVYTAFLDKLRSKHPGVRFLLLS
                     TKVWPSDDLTSRVESIVKSQKEKGHTDVEHHLMISENTALDGHPSENAHQNMANQIRP
                     IIARLGKWLQR"
     gene            3110..4399
                     /locus_tag="PLT31_00320"
     CDS             3110..4399
                     /locus_tag="PLT31_00320"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPW93607.1"
                     /translation="MNILIFALLSITVITVLVYRNGMDKIYNINLANYPYVIPTTDSV
                     DGGQSAISMARTDSSIIIDYELKEGYPYPYAGVQIYLGDGKSQGKNFAEYDSIFLWLK
                     PRGEGSVRLYLRGWDSAISKVEEPTSLKFNELEFFPLDETYPAVFVPEEFRVAGWWVA
                     QHNVNVHRARVDLSNIPLIEIQTGTAAPLGYGTLEIKGISFKGKIVSELKVATILVSI
                     WFITFFIILVIRFLDYSREHAANKRKREELEKNLKALEIEKADFEKLSKEDPLTGCLN
                     RAGFTSVLTREQERLNKQGHPVSFVILDIDHFKDINDTYGHTTGDDVLSNLSKLIQSK
                     IRNSDVLVRWGGEEFVILCGDTPLQNAQFLAEKLRQAIESAELIPERKITCSFGVAEM
                     IPDEDAKKLFDRADKALYASKESGRNRVTSASFKKTK"
     gene            4409..4924
                     /locus_tag="PLT31_00325"
     CDS             4409..4924
                     /locus_tag="PLT31_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93608.1"
                     /translation="MKKTLFFINFLFVSLLLLSSCAKKEILFVPLKIEWFEYKGDLSK
                     IDQSLVPAPVVEGFDSTENRCFIAMTKILMEDPLIQLRANQNISFDVQYYRYQYKKEV
                     SIHFLGRLSEPEKQGTLETEPMVLPLFPKEDIGAWTARCNPKGQIKDFAFNGQKTKRQ
                     RPLKMKTSTIE"
     gene            5041..5877
                     /locus_tag="PLT31_00330"
     CDS             5041..5877
                     /locus_tag="PLT31_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02147 family protein"
                     /protein_id="HPW93609.1"
                     /translation="MKTIFEYTDYREWLRDAFEEFKRQKTVISWRYMAMKLNADPGNL
                     LRISQGKIHLATTMIIPMAKFFGLNDKETAFWTELVYFGRAKTDKEALSHYEKMQILK
                     GIPLKRLEKKELEFYRHWYYNAIRSIIGICNFKDDYLGLAEACTPVITEKQARESVIL
                     LSALKMIKQDEGGFWRVTDTFVSTGGNWRSEAVSSFQKETLSLAKESLDRHHASLRDI
                     STVTMTFNIDEISLIREKIKEFRTELLRMSQEGSNDNAVFQLNVQMFPLAIVKKMKEK
                     SS"
     gene            5874..7010
                     /locus_tag="PLT31_00335"
     CDS             5874..7010
                     /locus_tag="PLT31_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93610.1"
                     /translation="MMNRLLLLFVILLSFSCSKPEDPISGGPGSETTNGIVAQIFDSK
                     GKPASKAGVALRRVDFSPDSASIFKDAIMPDEYTDSLGFITIDSILDGSYRITIEQDG
                     EMLSREIDFKDSLDLGKVHLRKTGSFNGSIALPTKHKFGWVGIYGLDILVKTDSLGNF
                     SLPELPASDDSMSLYIRSDSFDDTLREKTIFIEPETEVNEFKGIVLQDFDKTSYDYWY
                     ITPDTVGAVLKSPSSFSEGIVYDDTRKSNVFHASYEMGLGWSWVVIGTSIEHGPLNFS
                     QLDSISFWAKGNGLIRVQLENWTVLSELEGSNLKTYTPYKLLTEDTWEHIVIKPENFC
                     EINDVYTKCDSWETTKGSVQQIHFFLNSGTDIYLDDITLYGIRF"
     gene            7056..7943
                     /locus_tag="PLT31_00340"
     CDS             7056..7943
                     /locus_tag="PLT31_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPW93611.1"
                     /translation="MQKLENEAAFASKYLSQIAKKAELKFEEHLPPSSDIPHKLHEAM
                     RYSMFAGGKRLRPGLAFAAFELFGGKDDTIWYATSALEMLHTFSLIHDDLPCIDNDDF
                     RRGKETAHKKFGEGTAVLAGDALCILAFELIAKTGKNKPIQTLAKALGSYGMIGGEIT
                     DIECEGKKVDLATVDYIHYHKTAALIEASLMIGAQMADASEKDVLTIQRFGKAIGLAF
                     QVVDDILDIVSTTEELGKDAGSDEEKGKATYPAVIGLEASRERAFDLYNEAITILKDL
                     PGDTSVLQSIAAFIITRVN"
     gene            7943..9811
                     /gene="dxs"
                     /locus_tag="PLT31_00345"
     CDS             7943..9811
                     /gene="dxs"
                     /locus_tag="PLT31_00345"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HPW93612.1"
                     /translation="MLLKEIHSPKDIKGCTVEELNELAKQVREKIISQIAKHGGHLAS
                     SLGVVELTIALHYIYNTPEDILVWDVGHQAYVHKLLTGRYDQFETLRQQGGLSGFLKR
                     TESPYDAFGAGHASTSISAALGFAVARNHLKKNHNVVAIIGDGSMTGGMAFEALNNAG
                     ISKENMTIILNDNKMSISPNVGGFSKYLNRIISDPVYNKMRVDIDKLMSRLPGVLGSR
                     FRDLFLATENAAKSALKPGRFFEDLGVRYFGPIDGHDLNELIQILDRVKNIPGPCIIH
                     VLTEKGRGLSLAEKNPSKWHASVPFDPESGLPLNKCNSNKSLTTIFGETLLDLVKKDN
                     RILCITGAMASGCGLNIVEQELPDRVIDVGIAEEHAVTFAAGLACNGVIPVVAIYSSF
                     MQRAYDQMIHDVALQNLHVVFILDRAGLVGADGPTHHGSFDLSFLRTIPKMIIMAPSD
                     ENELRDMVTASIDMPGPVAIRYPRGNALACELSTEVKTIPIGKPIVIESGSDILLLGA
                     GFMLQELKKTASILRTQGKNPTLVDARFIKPLDENAYEELLKTHSVVVTLEDNTLIGG
                     YGSAIAELMSDKGYTSHKLLRFGLPDDFVEHGNVNDLYKQLKIDGESVAQKLMEIL"
     gene            9808..11214
                     /locus_tag="PLT31_00350"
     CDS             9808..11214
                     /locus_tag="PLT31_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HPW93613.1"
                     /translation="MTDENRPNKRMVKTTFNRKFGVSESVADKGFGRRPEREDRSYGE
                     KRSFGDRKPFGEKRSFDDRKPFGEKRSFGDRKPFGEKRSFDDRKPFGEKRSFGDRKPF
                     GERRSFDDRKPFGEKRSFDDRKPFGEKRSFGDRKPFGERRSFGDRKPFGERRSFDDRG
                     RRPLNRPYEVWQENPSEVQEPIYRQKPVTEEPIVFENDVELDITTIDAEPTTPPWFKK
                     ILALTTDKGREREGKFLAEGIHVIQELLNHHADLIEAVYFVEDFKDEVLINEIKAKEI
                     RTHDLPADLMKRISTTVTTQGIIATCRIASTKPDYDKSRSVLTLVDAIQDPGNLGALF
                     RTSLGFNSSGMILGKGTVSPFNSKVVRGSSGTFLRVPFEASVDLIEQINFLRQKGYTI
                     IATDLHAKQTLSDIVPRKLKKVAFLVGNEGAGTKSYLIEIADESVRIPMNNNLESLNV
                     SVAHGILSYEVAQLQKDM"
     gene            11217..12083
                     /gene="pyrF"
                     /locus_tag="PLT31_00355"
     CDS             11217..12083
                     /gene="pyrF"
                     /locus_tag="PLT31_00355"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HPW93614.1"
                     /translation="MVSQNFSSFLEKRIAECGNPVCMGMDPVLDLIPLEGTPEEKVKR
                     FYFEILEEILRRGVSPAVIKPNSAYYECISIDALRVLQDIIKVYQSAGIPSILDAKRG
                     DIGKSSAAYARAAFDVFGADAVTVSPWMGSDSVAPFLPQGGFKGVYALLRTSNKGAAD
                     FQDLKTKEGSTAFYAVAEKLMAWDNGNLGAVVGATHPEELEAITRFFVEHNHEIPFLI
                     PGVSIPGVPGGQGGDAKTVLNAINNGGGKRQFHVLNSSSGLNFAWQAKGNPSSFAVAC
                     VDALERLTDSLR"
     gene            12104..12886
                     /locus_tag="PLT31_00360"
     CDS             12104..12886
                     /locus_tag="PLT31_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93615.1"
                     /translation="MRFIISILISVVIIATAYLFSKPLPDTGLDESFLPKAEYVRYLS
                     AGHEVTASGLFWIRGLIDLGGSYLSGEEYAYLGHVGELATQLDSLFYTPYYFVGGVTP
                     ISSKDTSDFSVMRRAHRVFPEDWRLALYFAIRLANGPFKNNAEAAEIMRSYSTSKDTS
                     MPEYIRKIHRTFEIGAMQTEIAIQTILEDCVKPEYKGFKNSFYAKTIRVLGYDPLEKR
                     PEIEEIKKTIDGIIAQQMHPQYAYERLLRLKKEPEETTEIKE"
     gene            complement(12876..13658)
                     /locus_tag="PLT31_00365"
     CDS             complement(12876..13658)
                     /locus_tag="PLT31_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93616.1"
                     /translation="MVKNIFVFLLLFLSVASSSESGVLSFRAKQSLAAGRYAKAYTQL
                     EKALLATRKESDLLAEGRVLIAMAQIRTQSLDFVLADSLLSVVRQDALDTASIVAWVQ
                     AKMMLLNAQEKYDKTLKIAKTVSEDMLDESPDILQGYFWSEQALALIALKKSQEARSI
                     YKKAKKELSEDKSSYDLIQARAAHLEKRFSQADSLYAEAAAKAIEENNTYISASILFY
                     RAQIALKQQRISDAKDYLKRSAQAFELMGLPNNQKRSERLIP"
     gene            complement(13652..14611)
                     /locus_tag="PLT31_00370"
     CDS             complement(13652..14611)
                     /locus_tag="PLT31_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPW93617.1"
                     /translation="MKVVRQINWTELSGLLVGVFFAVALIVFGVTFYTKLITSGIIGV
                     EEYKLYSNFEKALGLHTGTKIQISGVDVGRISDMYIQESGVLMEFTIRSEYKNWITDS
                     AMVFAIRDQNVISARVINIDVKKGKGRILEDGDYLSAGKAQDIETVLETTNELLLRVN
                     VLIDAADTLVEMAMDTGTTIGALLGSRLLYDNLNQQLMRLDRMTYTGTNILNNLHVRM
                     PGLFDRMDTITEDVIVLLGDVKDVPQKLDRTFTSLDTVLYRMDGLVGQLGGVTVSLDQ
                     FIGNGEQTLQSAEDLITSISNMWIIRRSIPKKDSIPFVVETLW"
     gene            complement(14773..15591)
                     /locus_tag="PLT31_00375"
     CDS             complement(14773..15591)
                     /locus_tag="PLT31_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW93618.1"
                     /translation="MALVIEDLHFTYSEMMSRRKVRSQGFLNWRGLLGAVLEDSKKEV
                     LSGVNLKVKLGETICIGGASGQGKSAFLRIIAGLAKPTSGCLYYFGEHIPIERHNALE
                     IASRQVGLVFQNGALISNLKVRDNVALPLRYHGMGTEAEIDDKVNMALDLMRVRREAD
                     LFPHMLSTGMQKRVAIARSWAMDPKLLLMDEPTSGLDNYNRRNLLPLIDNMRKLFNTS
                     IIIVTHDLLIAKELECDILFLHDKQLSEPHSFDYWLKSKEPIAEELFRDLQAKT"
     gene            complement(15600..16472)
                     /locus_tag="PLT31_00380"
     CDS             complement(15600..16472)
                     /locus_tag="PLT31_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW93619.1"
                     /translation="MKNYVVYKKLTLPGRFFCWLGVVFLSTTFGQQFTLFIKTVASIF
                     SRNRNFKTDSKNIINQIFFTGIEIFPVLFVVATLFGTVIIIEVITMMNKVGFSDVVGN
                     LMVIVIIRELGPIFTAFLIAGRSGSALTTYIGSMAINSEVDSLATMGVDPIRYLVMPA
                     LIGGIFAVLIMTILFSFSGICAGFLTAKVLIYFAGDMLNVQISWSYLSTAILQSLTIT
                     DFILVIVKPVLFACIIITNASYQGLAIRKDVRQVPKATSRSVIYSFLYIVVFDVILSF
                     FYIFQYFGEISKII"
     gene            complement(16474..17082)
                     /gene="yihA"
                     /locus_tag="PLT31_00385"
     CDS             complement(16474..17082)
                     /gene="yihA"
                     /locus_tag="PLT31_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HPW93620.1"
                     /translation="MSEVKIVGDFQILSAEFVKSAVDLKDLPEERLPQIAFLGRSNVG
                     KSSLLNALMGEKNLVKTSAIPGKTREINFFKVNGAFFLVDLPGVGYAKVSIKQRDLMA
                     DSIREYVEKAEDLRGVVYLVDIRHAGTAIDILTVEELRKTGVPVLIVASKRDKVNQSE
                     FNKNKKVIQEKFGLAVPPITVSSVSKVGLSALWFELLNVIRE"
     gene            complement(17079..17672)
                     /gene="recR"
                     /locus_tag="PLT31_00390"
     CDS             complement(17079..17672)
                     /gene="recR"
                     /locus_tag="PLT31_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HPW93621.1"
                     /translation="MIEPPSLEELIAEFSSLPGIGNKTARRLAYHILSRPREQAVRFS
                     ESILKAADHVKPCSRCYVFTEDEICSICRDRSNAKTICVVEKSSDILPFERSGVYQGL
                     YFVLGGVLSPLDGVGPEALHIPLLIERVKTEGIQEVILGLGSSPEGESTALLIDRLLE
                     GASVKRSRLARGIPMGSDLEFVDEITMLRAFEGRVIF"
     gene            complement(17665..18894)
                     /locus_tag="PLT31_00395"
     CDS             complement(17665..18894)
                     /locus_tag="PLT31_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPW93622.1"
                     /translation="METLAYLARQPILNRDGKIFAYELLFRDSPISESAVIHNGVQAT
                     AQVLENVLNNIGLSKLIGNHKAFVNCSREMLLDNLFCLLNPDRFVLEILETVKPDEAV
                     IRAVEQYHKKGFQLALDDFIYTPETIDEWAPIFPYISYVKMDLVDNTPKNRALAASYF
                     KSRGIYVLAEKVETENDFNECLKEGYDFFQGFFFAKPELVTSRKMDSTTSAILDLLQK
                     LRSEQNLDDISCAFEKYPELIHKLLSYINARDSFKKPLVSIREALIQIGIKHLNHWLL
                     LLLYSRPEMGDSPQSSPLFQNASHRARFLENLGKAIDPEGDLAAKAFIVGLMSRMDAL
                     FKMPFQNILPSLSVDKDIQEALLLKKGRLGLLLRLADAVELDLHREIRICSEELGLSS
                     EVLTQCIHDAYIWIMDD"
     gene            complement(18904..19743)
                     /gene="hisG"
                     /locus_tag="PLT31_00400"
     CDS             complement(18904..19743)
                     /gene="hisG"
                     /locus_tag="PLT31_00400"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HPW93623.1"
                     /translation="MIKVALPNKGMLFEPTQELLKACGYKANKPYKTLTSIDTQNGIE
                     FFFLRPSDIPMYVGRGIIDAGITGIDFNAEANSPAVKVLDLPYGASKLCAAVPNESQI
                     QNLDELKDSNIATSFPGIVNHFYKKKMNVIELEGAVEISVSLGVADAVVDVVETGTTL
                     KQAGLRILGEPLFRSNAALFCHPEKQYETEVHTLIRRIEGKLVAQSYMMIEYNVPAEL
                     LSMACDITPGLDAPTISKLHGKDWYAVKAMVKQDDANQIMDKLWDMGCRSVLLFGIKS
                     ARI"
     gene            complement(19740..20000)
                     /gene="hisE"
                     /locus_tag="PLT31_00405"
     CDS             complement(19740..20000)
                     /gene="hisE"
                     /locus_tag="PLT31_00405"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HPW93624.1"
                     /translation="MTFEEMYALACRRKEEMPEGKGTTELFRKGPHAIGKKLVEEAAE
                     SWMAARYETRDDQALELSQVLYYVAVMMAEKGLTLEEVYKKL"
     gene            complement(20160..20840)
                     /locus_tag="PLT31_00410"
     CDS             complement(20160..20840)
                     /locus_tag="PLT31_00410"
                     /inference="COORDINATES: protein motif:HMM:NF014447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPW93625.1"
                     /translation="MSSENKVRRVVSNQTEIFSDLEKKVLRYLKTTYQRPYASHTIKA
                     FNQAQDFIRTFEKPVVLDSGCGCGESTLVLAAKYPDHPVIGIDKSQIRLTKAQKNSQP
                     KNVFYIQAELIDFWRLALEAELNVFFHALYYPNPWPKESQAGRRFHGHPIFPTLLQLC
                     PNMELRTNWKIYAEEFLEASRIGFQYLQMKWTPLLEEWVPSVPETAFERKYFENGQTL
                     FRVRIVRD"
     gene            complement(20844..21986)
                     /locus_tag="PLT31_00415"
     CDS             complement(20844..21986)
                     /locus_tag="PLT31_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPW93626.1"
                     /translation="MRNLFLLGSLLVFILGCHSEVAGGGPSGTEAGNAITAQILRGNK
                     TPAVAARVFIMPSSSILSTEEQTLKTTVTDSEGRFSVQDLAVGSYTVEVSDPSGALQF
                     NAVVYDSLSFDRGIDTLRSFSKIQGSVGFASAGFVTVQGMRHRAPVDANGLFSLDSLP
                     SGSASLVFSQGSESTPYYSYAVLLPGDSIEPSSFVQETDRLLLEDFEDMNTQHRFAPY
                     VGDSTGWWFLSAHEEVDVLFVDSLLVGYPLVKEESQYIAFNVTVPETVSVPWVNFGIQ
                     IGGNSSVYDLTGLDSVAFKAKGSGTVFFQLIGVEEDSSVVTPWPEQRFDLPSEWTRFA
                     FSVNDFTSNPALLKGIRVVSWVFTSSSDFALDDVELIGLSKEDLWF"
     gene            complement(21986..22837)
                     /locus_tag="PLT31_00420"
     CDS             complement(21986..22837)
                     /locus_tag="PLT31_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02147 family protein"
                     /protein_id="HPW93627.1"
                     /translation="MPDILDYLEYREFLRDWFIESKKENRFTSYRYLGQKTGVDPAWL
                     VRVFQKEGHLNEEALPAFIRLCSFDDRRAEYFRILYRFCKTKAKASLSELYCRLMELR
                     EMEARVLTSPEFMYFGNWACTALRALVGISQDTSDLTVLANHLNPPISQKDAHNALEV
                     LKQLKLIEPDGHGGWNITQQIVTTGGEVKSRAVRDFHRRTLELAQESLDRHSVAERDV
                     SSIVFTVDEADIPEFKQRIEDFRRGLLQFAKRSENANRVYALNVSMFPLSDVIENPAS
                     SDPKEVS"
     gene            complement(23132..23722)
                     /locus_tag="PLT31_00425"
     CDS             complement(23132..23722)
                     /locus_tag="PLT31_00425"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPW93628.1"
                     /translation="MNKILMSFMVLAMLATTSFAQNVGGHLALNFGTTIGEYNEDVSW
                     GLGVNAGVAAKFTLIPMLDLAPEIDFEMRRASDDDVTWTTMAINIPVLLRVNVMPQLF
                     LEVGPTVAFLLSSEMETEVTELGDVATVSMDLGSDEIDVLETFELGLAAGAGFTVMPK
                     LDLNFRVAMGLTSLVSDDFGEAKNLQLQLGGTFWFL"
     gene            complement(24203..24517)
                     /locus_tag="PLT31_00430"
     CDS             complement(24203..24517)
                     /locus_tag="PLT31_00430"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HPW93629.1"
                     /translation="MNTSDQEVRSVRVTVGQEKFQIQTDLNDAEIEEIVGFVTKKMDQ
                     HLKSNPRLEIRKQLILVAMDIASELFDTRRRLKKAHIYYQESQKTAGILASMLEEESS
                     SL"
     gene            complement(24565..24942)
                     /locus_tag="PLT31_00435"
     CDS             complement(24565..24942)
                     /locus_tag="PLT31_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93630.1"
                     /translation="MNLENIDLLTQKVERVLEVIRVLRAEKAQMESKIQSFDSVLAQK
                     DEEIAMLKSSLEERSVELTSLNDTVKNQEEEIQLAQERFQQLLSTIEAELGTEISIAP
                     SEQDSSSVPNEQQEEAPQSDFFS"
     gene            25049..26875
                     /gene="uvrC"
                     /locus_tag="PLT31_00440"
     CDS             25049..26875
                     /gene="uvrC"
                     /locus_tag="PLT31_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HPW93631.1"
                     /translation="MKTFALEERLSQLPAKPGVYIMKNADGEIIYIGKAKVLKNRVRS
                     YFDGREKNGHRAASLMLPHIHDIEWIITETEAEALILEATLIKKHIPIYNVRLKDDKH
                     FPYLVLTVREPFPRLILTRSIRSDGNLYFCPFLNSRAIHHLVDIAARLFHIRECHLKL
                     PGKDVIRPCLNYHIGRCAAPCAGLCSQEEYSKGVHDVTLLLEGKRNDLISEWEREMEE
                     ASLKLDFETASKKRDAINAVKALNSRQKADVSDASLTLDVISVKRHQKLSTAVILEYR
                     AGVFYGRRHFNLECNLEQSESEILEEMIGSWYFDNEEIPKEIAIDTELPEDHTLLEEA
                     LSAKAKHKVSIIRPQRGEKLGFLKLAQANAEMLLVEMIASIQKYDDVEQSVFTLQQEL
                     QLKNTPFRIECVDISHLAGTHTVASLVSFKNGKPDKSNYRKFIIKSVSGVDDFASMRE
                     VLTRRIERLHKENIPMPDLFVMDGGKGQVEATASILRELNEPDVPLIGLAKRLEEIVF
                     PGNTPSKILRRQSPALQLLQKVRDEAHRFAITYQRSKRILDLKVEWLSLPGIGTATRK
                     KILSKYRSREAFLKAPKEDLEILLGKKKSLVIYKTISEYKEK"
     gene            complement(26932..27840)
                     /locus_tag="PLT31_00445"
     CDS             complement(26932..27840)
                     /locus_tag="PLT31_00445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HPW93632.1"
                     /translation="MKMKVFSLLLLSFSLFLSACLFDSETEEDDVKSSSSTEVVSSSS
                     SSSIKVTSSSSTGTTSKSSSSGEVIKSSSSSVPKESSSSQWVEYPYYSSGIFCFTEGC
                     EDNLSSSSEAPSSSSVNSSSSVIIPSSSSILQGDGVIEDGVMVDKRDNQTYEIITLND
                     KIWMAQNIGFETPSGSFCYEDKETNCDVYGRLYVHEAALLACPGGWHLATRSEFEAAM
                     QEPTFIWNYNGRMSNNSYNFLDGMGFNWASGTMDSADKDNCDGSTNCSLIFVQKNPDS
                     EFAADPALFFQKDYKTKGFSVRCVKN"
     gene            27908..28300
                     /locus_tag="PLT31_00450"
     CDS             27908..28300
                     /locus_tag="PLT31_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HPW93633.1"
                     /translation="MSNIAIFDTTFAVTVLTIIALGVPFILLFANWLLHPGRIKRTLI
                     KGSAYECGLAHVSGTANDRYPVKYYLVAMLFLVFDLEVAFLYPWTIQFLKGGWDLFFI
                     LLGFLFILEGGYFYLYKKGVLDWATLKD"
     gene            complement(28305..>28857)
                     /locus_tag="PLT31_00455"
     CDS             complement(28305..>28857)
                     /locus_tag="PLT31_00455"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPW93634.1"
                     /translation="GGWLTGLGYTLSLDTRDHPVLPSHGFYAYWQQVFYNNAFGDSSY
                     IFQELDLRGYTYLFWKTSLALGALYQMTFGDVPFDQLAMPDGTKRFRGVERGVFRDRQ
                     AFVWQAELRRPLFWRLAGTIFYETTKVGPYFGALYREKWHHAFGFGGRLALNKKERVF
                     ARCDFSLIDGKHLGMSIYIREAF"
BASE COUNT         9228 a         5791 c         5111 g         8727 t
ORIGIN      
        1 agcaagtaga gcttctactc gatcaaaaac gggttcacca atggatgctg gtggcgacga
       61 actaaaagag aaacaacagc accaatacta tcatgattgt ctttgacgtc gctaattact
      121 ttttcaaagc gagcacttgc ttctggatca ggacaacgaa caggagacgc ttcgattgca
      181 tcataagtca ctttattgag atccacatga ctttcttcgg cgcctacaga agagacccaa
      241 gcaataattt ctgttccata tcgagtttct aaaatttttc gagcaatagc cccagccgcc
      301 actcgagcag ccgtctcacg agcagagctt ctgcctcccc cacgataatc tcgaaaaccg
      361 tattttaagt cataagtcaa atccgcatga ccaggacgat accattgagt catatccgaa
      421 taatcatggc tacgttgatc ttcgttaaaa atgataagag aaattggtgt tcctgtggtt
      481 tttccatcaa aaacgccaga taaaatatct actcggtctg cttcctgacg tggagtgacc
      541 attgcatttt gacctggccg acgtcgatcc aaaaactttt gaatatcctc tgcacaaaga
      601 ggcacccctg caggacaacc atcaatgaca gcaccaattg ctttcccatg ggattctccc
      661 caagtagata cagtaaactc ttttccaaat atacttgaca tatccataat ataataaaag
      721 gtcaaagtta ataactcttt aagaaaagcg tcttctctta tttcagatat tttttactat
      781 gttttgtgga ggtatcaagg aggaagaatg caaaagaaag cattcctttt tataattctg
      841 actctgttac tcgtaacgga agtattttct caaaaactgg atcagtttgc cggtatttct
      901 tttggtctta acagacaaga agtgattgag gaaatcatga aactcggtta tgacccttat
      961 ggtcaagttg gctcggccag cgatcgagtt attattcctg tgtatatgct cgcagaactc
     1021 cctgttcaag tcgattttct ttttaataaa aatgaaaaat tttatgcttt tgaaattaga
     1081 actggccgta tggaacaaat gcgattaaat aaggtttttg aagctgttgc ctatatgtct
     1141 gatcatttta ctgtaaaata tggaaaacct tcaaaagtgc cttccttaaa agaacaagat
     1201 attaaggaga atgttcataa tatttatcaa gaatggtttg gtgttaaatt tttagatatt
     1261 tatacagcta ttattaaaaa agatgggcgt ttttatacca ttggttcagt cactcacaga
     1321 acccttgcca aagaaaagaa ttctgaaggc aaaaagaatc tctcttctaa agaaagagta
     1381 cctgcatttt agcttttatc attacgttta ttactcaggt aaagtctgcg caaatcccgc
     1441 aatatcgatc atatcttcta gagggatttc atcaatatag ggaagcattc cacggccacc
     1501 atcgccaaaa ttcatcaaat accaaaacgt aggcatttca tttcttgctg tagtacctaa
     1561 ataagtagct gtttttttgt ttggagtaaa attgactcga cgtccatcat ctccatgaca
     1621 agccctgcaa ttcatgtaaa aaagctctgc cccacgcttt aaattggccc cttttattct
     1681 tttattctca tctaataagt tgtaaatcaa atatgccata cgagcatctt ctctagtcag
     1741 agtatctgct tttgacccag gagctggttt atccatcatt gggttcaatg agtttgtagt
     1801 atcctcagca aacaccatgt ttccaagaag gatgccataa aacattataa aaatggtgct
     1861 caaccatatt ttttttgtct ctctcataac accaatttat attttctgat gcatgaatag
     1921 atgtttatcc ttttttttat ttttctttat gactcttaat ttttcaattt cttccgctgc
     1981 tacaattatt cccagagatt cttcttctct tgaattttat ggacgctggt taattgaaaa
     2041 ggatcaagcc cgaactagcg caccaggtgc aagtgtccaa ttttcttttg aaggaacttc
     2101 tatttatgct gagctagaag gcgaatccta ttggacagta gaaatagatg gtaaagaata
     2161 cccatccata ttaacaggat ctcgtcaaaa atacgctctt gcagaaaact tacctatcga
     2221 aaaacatcaa gttactttat ctaaaaagtc agaaacaccc acgacggaag taatccttta
     2281 ttctttatct acaaataaag aagggtcctt tgcaaaaccg cgtcgtaggc cagatcgtcg
     2341 aattgagttt atcggagatt catttactgt tggctacgga aacgaagcaa aaaattcaac
     2401 ggatggcgat gtttttgaaa agacaaatgc cacaaaaagt tttgcatatc ttttaggcca
     2461 aagccttcgt gcagacattc aaatcaacgc ctttagtgga agagggctag ttcaaaacta
     2521 tgccaatcat actcccgaat ggagtgcatt aaaattatat gaatacacca ttcctggcat
     2581 tgcggaaaaa caaccaaaca cgcctctttg ggattatagt aagtggcacc cccatgtcat
     2641 agtcattttt ttgggaatca acgattttca aggtgatttt gatcacacta aaccatccac
     2701 ttttgactcc gtttatacag ctttcttaga taaattacgt tcaaaacacc ctggcgtccg
     2761 ttttttactt ttaagcacca aagtatggcc tagtgatgac ctcacctcaa gagtcgaatc
     2821 tattgtaaaa agccaaaaag aaaaagggca tacagatgta gaacaccatc tcatgatttc
     2881 agagaatacc gctctagatg gtcatccaag tgaaaatgct caccaaaata tggctaatca
     2941 aattagaccg attatagctc gccttggaaa atggctccaa cgttaatttt taacgttttt
     3001 taatatgaca aagaagccag gatattgttt ttaatgtatc ttataaaaag gttgtaagtg
     3061 agcttagaga attaagataa tgttacaaaa gattctagcc ttgttcagaa tgaacatatt
     3121 aatctttgcg ttactgtcca ttacagtgat tactgtattg gtgtatcgta atggtatgga
     3181 taaaatctac aacattaatc ttgcaaacta cccctacgtg atccccacca cagactccgt
     3241 tgacggcgga caatctgcaa tcagcatggc tcgcacagat tcctccatca tcatcgacta
     3301 tgaattgaaa gagggctatc cttatcctta tgcaggtgtg caaatttatt tgggtgacgg
     3361 aaaatctcaa gggaaaaact ttgctgaata cgacagtatc ttcttatggc ttaagcccag
     3421 aggagaaggg tctgttcgat tgtatctacg tggatgggat agtgccattt caaaagtcga
     3481 agagcccact tctcttaaat ttaatgagct tgaatttttt cctcttgacg aaacctatcc
     3541 agcagtcttt gtccccgaag aatttcgtgt agctggttgg tgggttgccc aacataacgt
     3601 gaatgtgcat cgagctcgag tggatttatc aaatattcct ctgatcgaaa ttcaaactgg
     3661 tactgctgct cctctcggtt atggaaccct agaaatcaaa ggtatttctt ttaaagggaa
     3721 aatcgtttcc gaattaaaag tggctacaat tcttgtttca atttggttta tcactttctt
     3781 catcatttta gtgattcgtt tcttagacta tagccgtgaa cacgccgcca ataagaggaa
     3841 gagagaagag cttgaaaaga acttgaaagc actagaaatt gaaaaagctg attttgaaaa
     3901 attaagtaaa gaagatccac tcactgggtg tctcaaccgc gctgggttta caagtgtatt
     3961 aactcgtgag caagaacgtc tcaacaagca aggtcatcct gtttcctttg tgattctcga
     4021 tattgatcac tttaaagata ttaatgatac ttatggtcac accacgggtg atgatgtgct
     4081 ctcgaattta tctaaactca tacaaagtaa gattcgcaac agtgacgtcc tagtccgttg
     4141 gggcggtgaa gaattcgtga ttttatgtgg agatacccct ctccaaaacg ctcaattcct
     4201 tgcagaaaaa ttaaggcaag ccattgaatc agcagaactc atccctgaac gtaaaatcac
     4261 ttgttccttt ggtgttgcag aaatgattcc tgatgaagat gcaaaaaaac tttttgatcg
     4321 tgcagataaa gctctttacg cctctaaaga atcaggaaga aatagagtca caagcgcttc
     4381 atttaaaaaa acaaagtaaa tggattatat gaaaaaaact ctttttttta tcaattttct
     4441 ttttgtttct ttactcctat tatcctcttg tgcaaaaaaa gaaatccttt ttgtcccctt
     4501 aaaaattgaa tggtttgaat ataaaggcga tctatctaaa atcgatcaaa gtcttgttcc
     4561 agctccggta gttgaaggtt tcgactccac ggaaaacaga tgctttatcg ctatgacgaa
     4621 aatattgatg gaagatcctc taatccaatt acgtgcaaat caaaacattt cttttgatgt
     4681 tcagtactat cgttaccagt ataaaaaaga ggtcagtatt cactttttag ggcgattaag
     4741 tgaaccagaa aaacaaggaa cattagaaac tgaacctatg gtgcttcctc tttttccaaa
     4801 agaggatatt ggagcttgga ctgctcgctg taatcctaaa ggccaaatca aagatttcgc
     4861 ttttaatgga caaaaaacaa agcggcaacg tccactaaaa atgaaaacga gtactatcga
     4921 gtaattattt actttttttg acttctaatt attgtttttt gtcacttgtt gtaaaaagga
     4981 acaacacgct tcttctataa aaggtcctct accttctctt tttgaggtaa ttttattaat
     5041 gtgaagacta tttttgaata tacagattac cgtgaatggt taagagatgc ctttgaagaa
     5101 tttaaaaggc agaagacagt tatttcctgg cgttatatgg caatgaaatt gaatgcggac
     5161 ccaggaaact tacttcgtat ttctcaaggt aaaatccatc tagcaaccac aatgattatt
     5221 cccatggcca aattctttgg cttaaatgac aaggaaacag ccttttggac ggaactagtc
     5281 tattttggac gcgccaaaac cgataaagaa gctctttctc attatgagaa aatgcagatc
     5341 cttaaaggca ttccattaaa aagacttgaa aagaaagaac tcgaatttta cagacattgg
     5401 tactataatg ccatcagatc cattattggc atttgcaatt tcaaagatga ttatttaggt
     5461 ttagcagagg cctgcactcc tgtgattaca gaaaagcaag caagagaatc ggttattctt
     5521 ttgagtgctc taaagatgat caagcaggat gagggtggtt tttggcgtgt gacggataca
     5581 tttgtcagca cgggcggcaa ttggcggtct gaggctgttt cttcttttca aaaagaaacc
     5641 ttatcacttg ctaaagaatc cctagacagg caccatgctt ctcttaggga tattagtact
     5701 gtgacgatga cttttaatat tgatgaaatt tctttaatcc gtgaaaagat taaagagttt
     5761 agaaccgagc ttttgagaat gtctcaagag ggttctaatg ataatgccgt atttcaactg
     5821 aatgtacaga tgtttccttt agcgatagta aaaaaaatga aggagaagtc atcatgatga
     5881 atcgattgct tctactcttt gttattctac tttccttttc ctgttcaaaa ccagaagatc
     5941 cgatttctgg cggtccagga agtgaaacga ctaatggtat tgtggcacaa atttttgatt
     6001 ctaaagggaa accagcatcc aaagcaggtg tagccctacg tcgtgtggac ttctctcctg
     6061 attccgcatc gattttcaaa gatgctatta tgccagatga atatactgac tctcttggtt
     6121 ttatcaccat tgattccatt ctagatggtt cttaccgcat caccatcgaa caagatggag
     6181 aaatgctctc tcgagaaatc gactttaagg actctcttga tttaggaaaa gtgcacctta
     6241 gaaaaactgg ttccttcaat ggttctatag ctctccctac aaaacacaaa tttggatggg
     6301 ttggtattta tggacttgat attcttgtga agacagactc tttagggaac ttctcattac
     6361 ctgaattacc tgcttcagat gattcaatgt ctctttatat tcgaagcgat tcttttgatg
     6421 atacactccg tgaaaaaaca atcttcatcg aacctgaaac tgaagttaat gaattcaaag
     6481 gaattgtact tcaagatttt gataaaacat cttacgacta ctggtacatt acgcctgaca
     6541 ccgttggagc cgttcttaaa tcaccatctt ctttctctga aggtattgtc tacgatgaca
     6601 caagaaaatc aaacgtattc catgcctctt atgaaatggg attaggttgg tcttgggtgg
     6661 ttatcggtac atcaatagaa catgggcctc taaacttttc tcaactagat tctatttctt
     6721 tttgggcaaa agggaatggt cttattcgag tacagcttga aaactggact gtattaagcg
     6781 agcttgaagg gtccaattta aagacatata ctccttacaa attacttaca gaagatactt
     6841 gggaacatat tgttatcaag ccagagaatt tctgtgaaat caacgatgtc tacaccaaat
     6901 gtgattcttg ggaaacaact aaaggaagcg ttcaacaaat tcattttttc ctaaattctg
     6961 gaacagatat ataccttgat gatataacgc tctatggcat aagattctaa agttttttac
     7021 tttaagattc cctgaaatta aatatcttta gtcttatgca aaaattggaa aatgaagcgg
     7081 ctttcgcctc gaaatactta tctcaaatcg ctaaaaaagc tgaactaaaa tttgaggaac
     7141 acttacctcc ttcttcggat attccgcata aacttcatga agccatgcgt tactccatgt
     7201 ttgctggtgg taaacgtctt cgtccaggtc tcgcttttgc agcctttgaa ctcttcggtg
     7261 gcaaagatga taccatttgg tatgctacta gtgctctcga aatgctgcac acgttctctt
     7321 taattcacga tgatcttcct tgtattgata acgatgattt tagacgagga aaagaaacgg
     7381 cccataaaaa atttggagaa ggcactgccg ttttagcggg cgacgctctt tgcattttag
     7441 cgtttgaact aattgcaaaa acaggaaaaa acaagcctat tcaaacctta gccaaagcat
     7501 taggtagcta cgggatgatt ggaggtgaaa ttacagatat tgagtgtgaa gggaaaaaag
     7561 ttgatttagc aacagtcgat tatattcatt atcataaaac agcagcctta attgaggcct
     7621 ctcttatgat tggtgcgcag atggctgacg cttcagaaaa agacgtttta accattcagc
     7681 gttttggaaa agcgatcggg ctagcctttc aagtagtaga tgatatttta gatattgtat
     7741 ccactacaga agaactcggt aaagatgctg gctctgatga agaaaaaggg aaagcaactt
     7801 accctgcagt gattggtctt gaagcctctc gagaaagagc atttgatctt tataatgaag
     7861 ccatcactat tttaaaagat ttgcctggag atacgagtgt tcttcaatct attgcagcgt
     7921 ttattattac tagggtgaat taatgctttt aaaagaaatc cattctccta aagacatcaa
     7981 aggttgtact gtagaagaat taaatgaact ggcaaagcaa gttcgtgaaa aaataattag
     8041 tcaaattgcc aaacatggtg gacaccttgc ctcaagcctt ggtgttgttg aattaacgat
     8101 tgctctacac tacatttata atactccaga agatatcttg gtatgggatg ttggccacca
     8161 agcttatgtt cataaacttt taacaggtcg ttacgatcaa tttgaaacac ttcgccaaca
     8221 aggcggatta tctggctttc taaaaagaac agaaagccct tatgatgctt ttggtgcagg
     8281 gcatgcctct acatccatct ctgcagcact aggttttgct gtagctcgca atcatcttaa
     8341 aaagaaccac aatgtcgttg ctattattgg tgatggttct atgactggtg gaatggcctt
     8401 cgaagcgtta aataatgcag gcatttctaa agagaatatg accattattc tcaacgacaa
     8461 caaaatgagt atttctccta atgttggagg cttttcaaaa tatctaaata gaatcatttc
     8521 tgatcctgtt tacaataaga tgcgcgtgga tatagataaa ctaatgtctc ggttgcctgg
     8581 agtcttaggt tctcgtttta gagatttatt tcttgccaca gaaaatgcag caaaaagtgc
     8641 attaaagcca ggccgttttt ttgaagatct tggtgttcgt tattttggtc ctattgacgg
     8701 ccatgactta aacgaattaa ttcaaattct agatcgagtc aaaaacattc ctggtccttg
     8761 cataatccac gttcttacgg aaaaaggacg cggactaagt cttgctgaaa agaatccttc
     8821 aaaatggcac gcctcagttc catttgatcc tgaaagtggc cttcctctaa ataagtgtaa
     8881 tagcaataaa tctcttacca ctatatttgg agaaaccctt ttagatctcg taaaaaaaga
     8941 caatcgaatt ttgtgcatta caggagccat ggctagtggc tgcggtttaa atattgtaga
     9001 gcaagaatta cctgatcgtg tgattgacgt aggcattgca gaagaacatg ccgtgacttt
     9061 tgcggcaggg cttgcatgca acggagtcat tcctgtggtc gccatatatt cctcttttat
     9121 gcaacgtgct tacgatcaaa tgattcacga cgtggcctta caaaatcttc atgttgtttt
     9181 tattctagat agagcagggc tagttggagc cgatggtcct actcatcatg gttcttttga
     9241 tttgtctttt ttaagaacca ttcctaaaat gattatcatg gctccgtcag atgaaaatga
     9301 attacgtgat atggttactg catctattga tatgccagga cctgttgcca ttcgttaccc
     9361 aagaggaaat gctttagctt gcgaattaag cactgaagtg aaaaccattc ctattggaaa
     9421 acctattgtt atagaatcgg gttctgatat tttgcttctt ggtgcaggat ttatgctaca
     9481 ggaacttaag aaaacagcct ccattttacg aactcaaggg aaaaatccaa cgttagtaga
     9541 cgcccgcttt ataaagcccc ttgatgaaaa cgcttacgaa gaattattaa aaacgcattc
     9601 tgttgttgtc accttagaag ataatactct tattggtggc tacggatctg caattgccga
     9661 actcatgagc gataaaggat acacttctca caaacttctt cggtttggat taccagatga
     9721 ttttgtggaa cacggcaatg taaatgacct atataaacaa ttaaaaattg acggcgaatc
     9781 cgttgctcaa aagttgatgg aaatattatg acagacgaaa acagaccaaa taaaagaatg
     9841 gtaaaaacta ctttcaaccg caaatttgga gtgagtgaaa gcgtcgcaga taaaggcttc
     9901 ggaaggcgcc ctgaaagaga agaccgttcc tatggtgaaa aacgttcctt tggcgaccgt
     9961 aaacctttcg gtgaaaaacg ttcctttgat gatcgtaaac cgtttggtga aaaacgttcc
    10021 tttggcgacc gtaaaccttt tggtgaaaag cgttcctttg atgatcgtaa accgtttggt
    10081 gaaaaacgct cctttggtga tcgtaaaccg tttggcgaaa gacgctcctt tgatgatcgt
    10141 aaaccttttg gtgaaaagcg ctcctttgat gatcgtaaac cgtttggtga aaaacgctcc
    10201 tttggtgatc gtaaaccgtt tggcgaaaga cgctcctttg gtgatcgtaa accgtttggc
    10261 gaaaggcgtt cctttgatga tcgtggccga cgcccgttaa atcgtcctta tgaagtatgg
    10321 caagaaaatc catccgaagt tcaagaacca atttacagac aaaaaccagt tactgaagaa
    10381 cctattgttt ttgaaaatga tgtcgaacta gatattacga ctattgatgc agaaccaact
    10441 accccacctt ggttcaaaaa aattctcgcc ttaacaactg ataaaggaag agaacgtgaa
    10501 ggtaaattct tagcggaagg cattcacgta attcaagaat tattgaatca ccatgccgat
    10561 ctaattgaag ccgtttattt tgtagaagat tttaaagacg aagttttaat caacgaaatc
    10621 aaagcgaaag aaataagaac gcatgatttg cctgccgatt taatgaaaag aatttcgacg
    10681 actgttacca ctcaaggtat tattgccaca tgccgaattg caagtacaaa gcctgactac
    10741 gataaaagtc gtagcgtttt aactcttgtg gacgccattc aagatccagg taatttaggg
    10801 gcattatttc gcaccagtct tggattcaat tcttctggca tgattttagg aaaaggaact
    10861 gttagtcctt tcaattcaaa agtagtgcgc ggttcttcgg gtactttttt aagagttcct
    10921 tttgaagcta gcgtcgattt aattgagcaa atcaatttct taagacaaaa aggttatacc
    10981 attattgcta ccgatttaca cgcaaaacaa acattaagcg atatagttcc tcgaaagctt
    11041 aaaaaggttg ctttcttagt cggtaacgaa ggcgctggaa caaaatctta cttaattgaa
    11101 attgcagatg aatcggttcg tattccaatg aacaataatt tggaatctct aaacgtttct
    11161 gtagctcacg gcattctatc ttatgaagta gcacaactcc aaaaggatat gtagaaatgg
    11221 tttctcagaa cttctctagt tttctcgaaa agcgtatagc cgagtgcggt aatcctgtct
    11281 gcatgggaat ggatcctgtt ttagatttga ttcctttaga aggaacccct gaagaaaagg
    11341 taaaacgctt ttattttgaa attttagaag aaattctgcg ccgtggtgtc tcgcctgcgg
    11401 tgatcaagcc aaatagtgct tattatgaat gcattagcat tgacgctctt cgtgttcttc
    11461 aagacattat taaagtctat cagtctgcag gaattccttc aatattggat gcaaaacgtg
    11521 gagacattgg taaatccagt gccgcttatg ctcgtgccgc ctttgatgta tttggagccg
    11581 atgcagtaac ggttagccct tggatgggta gtgattctgt agctcctttt ttacctcaag
    11641 gaggtttcaa aggggtctat gctcttttaa gaactagtaa taaaggagcc gctgattttc
    11701 aggacttgaa aacaaaggaa gggagtacag ccttctacgc tgtcgctgaa aaactaatgg
    11761 catgggataa tggaaattta ggggctgttg ttggagccac tcaccctgaa gaactggaag
    11821 ccattactcg cttttttgtg gaacacaatc atgaaattcc gtttctaatt ccaggcgttt
    11881 ctattccagg cgttcctggt ggccaagggg gcgatgcaaa gactgtcttg aacgcaatca
    11941 ataatggtgg cggcaaaaga caattccatg tattaaacag ctctagtggc ttaaatttcg
    12001 cttggcaagc caaagggaac ccaagttcat ttgctgttgc ctgtgtagat gctttagaaa
    12061 gactcaccga tagtttgagg tgaaattcat aggaggattt tctatgcgtt ttatcatttc
    12121 cattctaatc tctgtcgtaa taatagctac tgcttattta ttttctaaac ccctcccaga
    12181 cactggactt gacgaaagtt tcttacccaa agcagaatat gtgcgatacc tttctgcggg
    12241 gcatgaagtc accgcttctg ggctattttg gattagaggt cttattgatt taggaggaag
    12301 ctacttaagc ggcgaggaat acgcctactt aggccatgtt ggcgaattag ccactcaact
    12361 cgatagtctt ttttacacgc cctattattt tgtaggcggc gttactccaa ttagttcaaa
    12421 agacacctct gatttttctg tcatgagacg cgctcatcgt gtattccctg aagactggcg
    12481 tttagcactt tattttgcga ttcgactagc caatggacct ttcaaaaata atgcggaagc
    12541 cgcagaaatt atgcgttctt attcaacaag taaggatact tctatgccag aatatatccg
    12601 caagatccat cgtacttttg aaataggagc gatgcaaacg gagattgcca tccaaactat
    12661 tttagaagat tgcgtaaagc ccgaatataa aggctttaag aatagctttt acgctaaaac
    12721 cattcgtgtt ttaggttatg atcctctaga aaaacgcccc gaaattgaag aaattaaaaa
    12781 gactattgat ggaataattg ctcaacagat gcatccacaa tatgcttacg aaagacttct
    12841 tcgattaaaa aaagaacctg aagaaacaac tgaaattaag gaataagcct ttcacttctc
    12901 ttttgattat tgggtaatcc cattaattca aaagcttgag cgcttctttt taaataatct
    12961 tttgcatcag atattctttg ttgtttcaaa gcaatctgcg cacgataaaa taatatgcta
    13021 gctgagatat aagtattatt ttcttctatt gccttcgccg cggcttcagc gtataaagaa
    13081 tcggcttggg agaaacgttt ttccaaatgc gcagcacgag cctgaatcaa atcataagag
    13141 cttttatctt cagataattc ctttttagct ttcttatata tagaccttgc ctcttgactc
    13201 ttttttaatg caatcaaagc taaagcctgt tcactccaaa aatagccctg taaaatatct
    13261 ggagactcat ctaacatatc ctctgagacg gtctttgcta tttttagtgt tttgtcatat
    13321 ttttcttgtg cattcaaaag catcattttg gcctgtaccc aagctacaat ggaagctgta
    13381 tctaaagcat cctgtctcac tacagacaaa agagaatccg ctaaaacaaa gtctaagctt
    13441 tgtgttcgta tttgtgccat tgcaattaaa actcgcccct cagcgagtaa atcggattct
    13501 ttccgagtag ccaaaagagc tttttctaat tgagtataag cttttgcata tcgacctgca
    13561 gcaagagatt gttttgctct gaaagataaa acccctgatt cggaactcga agccacactc
    13621 aagaagagaa gcaagaagac aaaaatgttt tttaccataa tgtctccact acaaatggaa
    13681 ttgaatcttt tttcggtatg gagcgcctaa taatccacat attagaaatg cttgtgataa
    13741 gatcttctgc gctttgtaag gtttgttctc cattaccaat aaactgatct aaagaaacag
    13801 tgactccacc taattgcccc actagtccat ccattctata aagaactgta tctagcgaag
    13861 taaatgtgcg atcaagtttt tgaggaacat cttttacgtc acccaataaa acaatcacat
    13921 cttctgtaat ggtatccata cgatcaaaca aaccaggcat tcttacatga agattattta
    13981 agatatttgt ccctgtataa gtcattcgat ctaaacgcat gagctgttga ttcaagttat
    14041 catataaaag cctggatcct aataaagctc ctattgttgt ccctgtatcc atagccattt
    14101 cgaccaaggt atcagccgca tcaataagca cattcactcg taaaagcaat tcatttgtag
    14161 tttctaatac cgtttcaata tcttgggctt tacctgctga taaataatcc ccatcttcta
    14221 aaatgcggcc tttacctttt ttcacatcaa tattaatcac acgagcagaa atcacgtttt
    14281 gatctcgaat agcaaaaacc atcgcactat cggtaatcca gtttttgtat tcagaacgga
    14341 tggtaaattc cattagaaca ccagattctt gaatgtacat atcagaaata cggccaacat
    14401 ccacaccact aatctgaatt tttgtacccg tatgtaaacc aagcgctttt tcgaaattac
    14461 tatatagctt atattcttca acgccaataa tgccagaggt aattagtttt gtataaaaag
    14521 taacgccaaa aacgatcaaa gcaacggcga agaaaacgcc gactaataac ccagataatt
    14581 cagtccaatt tatttgcctt actactttca tctaagcccc tctctcaaca aatctaatga
    14641 gaaaagcctt aggtgtctca ggacacaaag aaatattctt cgataacaat tggctaaaac
    14701 aagatattgc ttccgatggg tccaagaatg aattagttaa ggagatcata ggttataaaa
    14761 taatataaat atctaagttt tagcttgtaa atctctaaaa agctcttctg cgataggttc
    14821 ttttgacttt agccagtaat caaaagaatg aggctccgat aattgtttgt catgcaagaa
    14881 caaaatatcg cactcaagct ccttagcaat cagtaaatca tgcgttacaa taatgataga
    14941 agtattgaat agttttctca tattatcaat tagaggcagt aaattacgcc gattgtaatt
    15001 atctaatcca gaagtaggct catccatcaa aagtaattta gggtccatcg cccatgatct
    15061 tgcgatagct acacgtttct gcattcccgt actcaacatg tgaggaaata agtcggcttc
    15121 acggcgaacg cgcatcaaat ctagagccat attcactttg tcatcaattt ccgcttctgt
    15181 ccccatgccg tggtatcgta aaggtaaagc cacattatct cgtactttta aatttgaaat
    15241 aagtgctcca ttctgaaaga ctaatccgac ttgacgactt gcaatttcaa gagcattatg
    15301 acgttctata ggaatatgtt ctccaaaata atagagacaa cccgatgtag gtttagcaag
    15361 gcctgcaata attcttaaaa aagcactttt cccctgccct gaagcaccac caatacaaat
    15421 cgtctctcct aactttactt ttagattcac tccagaaaga acttcttttt tactatcctc
    15481 aagaacagca cctaataaac ctctccagtt taagaaacct tgagacctaa ctttccttcg
    15541 actcatcatt tcactataag taaaatgcaa gtcttctatt actagagcca tttcaatcat
    15601 tatattatct tagagatctc cccaaaatat tggaaaatat agaagaaaga caaaatcaca
    15661 tcaaaaacaa caatatacaa gaaagaataa atcactgaac gtgaagttgc tttaggaact
    15721 tgacgaacat ctttacgaat agctaaacct tggtagcttg catttgtgat gataatgcat
    15781 gcaaaaagca caggtttaac gattactaaa ataaaatcgg ttattgttaa agattgcaga
    15841 atggccgtag aaaggtaaga ccaactaatt tgcacattta acatgtcacc agcaaaataa
    15901 atcaatactt ttgccgttaa aaagcccgca caaattccac taaagctaaa taagatcgtc
    15961 atgatcaaaa cagcaaaaat accaccaatc aacgctggca tcactaaata gcgaatggga
    16021 tccactccca ttgtcgctaa agaatctact tcagaattaa tggccatact accaatatac
    16081 gtggttaatg cagaaccgct tcgacctgca attaagaacg ctgtgaaaat ggggcctagt
    16141 tctctaataa tcacaatcac catcaaattt ccaaccacat ctgaaaagcc cactttattc
    16201 atcattgtaa tgacttcaat gataatcacc gttccaaaaa gagtggccac aacaaaaagg
    16261 acaggaaaaa tttctattcc cgtgaaaaaa atctgattaa ttatattttt tgaatctgtt
    16321 ttaaaatttc tatttcttga aaaaatagaa gctactgttt tgataaatag agtgaactgt
    16381 tgaccaaaag ttgtggataa aaaaacaacc cctagccagc aaaaaaaacg gcctggaaga
    16441 gttaattttt tataaacaac ataatttttc atttcattct cgaatcacat tcaacaattc
    16501 aaaccaaaga gcagaaagac ctactttaga gacgctactt acagtaatcg gagggacagc
    16561 taacccaaat ttttcttgta ttaccttttt attcttgttg aactccgact gattgacctt
    16621 atctcgctta cttgcaacga tcaaaacagg aacccccgtt tttctcaatt cttctacagt
    16681 caatatatca atcgctgttc cagcatgtcg aatatccacc aaataaacta ctcctcgcaa
    16741 atcttcagct ttttcaacat attcacggat tgaatcagcc ataagatctc tttgcttaat
    16801 actgactttc gcatacccta cacctggtaa atctacaaga aaaaaagcac cattgacctt
    16861 aaaaaaattt atttctcttg tttttcctgg aatagcgctc gtttttacca agtttttttc
    16921 acccattaag gcgtttaaca aagaggactt tcccacattg gaacgaccca agaaagcaat
    16981 ctgaggtaat cgttcttctg gcaaatcttt taaatcaaca gcactcttaa caaattcggc
    17041 agataaaatt tgaaaatcac ctacaatttt tacttcactc aaaagatcac cctcccctca
    17101 aaagctctta acatggtaat ttcatctaca aactcaagat cacttcccat tggaattcca
    17161 cgagctaatc tagagcgttt gacagaggca ccttctaata gacgatcaat caaaagagcc
    17221 gtactttcac cttctgggct tgaacctaaa cctaatatga cttcctgaat tccttcggtt
    17281 tttactcgtt caattaataa agggatatgt aatgcttctg gccctactcc atccaaagga
    17341 gaaaggactc cgcctaaaac aaaatataaa ccctgataaa cacctgatct ttcaaaaggc
    17401 aatatatcag aggatttttc tacgacgcag attgtttttg cattgcttcg atcgcgacaa
    17461 atagaacaaa tctcatcttc tgtaaaaaca taacaacgac tacaaggttt gacatgatct
    17521 gccgctttta aaatactctc agaaaaacga acagcttgct cgcgtggacg agacaaaata
    17581 tgataagcta aacgacgagc tgttttattt ccaatccctg gcaggctcga gaattcagca
    17641 atcaattctt ctaaactagg aggttcaatc atccattatc catatataag catcatgtat
    17701 acactgtgtt aaaacttccg aagacaaacc caattcttct gaacaaatac gtatttctct
    17761 atgtaaatct aattcaacag catctgctaa gcgcaataat aaacctaaac gtcctttttt
    17821 taataaaaga gcctcttgaa tatccttatc cactgaaaga gaaggtaaaa tattttggaa
    17881 aggcatttta aataatgcat ccatacggct cattaatccg acaataaaag ccttagcagc
    17941 taaatcccct tctggatcaa tcgctttacc taaattttca aggaatcgag cccgatgcga
    18001 agcattctga aataacggtg atgattgcgg ggaatccccc atttctgggc gactataaag
    18061 caataaaagc aaccagtgat tcagatgttt aataccaatc tgtatcaatg cttcccgtat
    18121 agaaactaaa ggctttttaa aagaatctct tgcatttatg taactcaata atttatgaat
    18181 aagctctggg tatttttcaa atgcacaaga aatgtcatcg aggttttgtt cagatcttag
    18241 tttttgtaat aaatccaaaa tggctgaagt tgtggaatcc atctttcgac tggtcactaa
    18301 ttctggcttt gcaaaaaaga atccttgaaa aaagtcatac ccttctttga ggcactcgtt
    18361 aaaatcattt tccgtttcca ctttttctgc caaaacataa atccctctgg acttaaagta
    18421 agaggccgct aaagctctat ttttaggagt attatccaca agatccattt tcacataaga
    18481 aatataaggg aagattggag cccactcatc tatcgtctcg ggcgtgtaaa taaaatcatc
    18541 taaggccaat tgaaaacctt ttttgtgata ttgttctaca gctcgaataa cagcctcatc
    18601 tggtttcacc gtctctaaaa tttcaagtac aaaacgatca ggattcaaca gacaaaataa
    18661 attatctaaa agcatttccc tgctacaatt cacaaatgct ttatgatttc caattaactt
    18721 agacagccca atattattta aaacattttc aagaacttga gctgtagcct gtacaccatt
    18781 atgaattacg gctgattcac tgataggaga atctctaaac aataattcat acgcaaatat
    18841 ttttccatct ctatttaaaa taggttgtcg cgcaagatat gctaaagttt ccatatagat
    18901 cttttatatg cgagccgatt taattccaaa caataaaacg ctacgacatc ccatgtccca
    18961 taatttatcc ataatttggt tcgcgtcatc ttgcttcacc attgctttca cggcatacca
    19021 atctttacca tgcagcttag agatggtagg agcatctagc cctggagtaa tgtcacaggc
    19081 cattgaaaga agttctgcag gtacattata ctctatcatc atataggact gagcaacaag
    19141 cttgccttca atacgacgaa tcagagtgtg aacttctgtt tcatattgtt tttctgggtg
    19201 acaaaataaa gccgcattac ttctaaaaag aggttcgcct agaatacgta gtccggcttg
    19261 ttttaatgta gttcctgttt ctactacatc cacaacagca tctgccacgc ccaaactcac
    19321 tgaaatttca acagcccctt ctaattcgat cacattcatt ttctttttat aaaaatgatt
    19381 tacaatacca gggaaactcg ttgcaatgtt actgtctttt aattcgtcca gattttgtat
    19441 ttgactttca tttggtacgg cggcacaaag ttttgaagct ccataaggaa gatctaagac
    19501 tttgactgca gggctatttg cctctgcatt aaaatcaatc cctgtaatgc cagcatcgat
    19561 aatgcctcga ccgacataca taggaatatc gcttggacgc aaaaagaaaa attcaatacc
    19621 attctgagtg tctattgacg ttaaggtttt atatggctta tttgccttat aaccacaggc
    19681 ttttagaagt tcctgagtgg gttcaaaaag cattccttta ttaggaagag caactttaat
    19741 cataattttt tataaacctc ttcaagagtt aatccttttt ctgccatcat cacagccaca
    19801 tagtataaca cttgtgataa ttctaaagct tgatcgtctc tagtttcata acgagcagcc
    19861 atccaagatt ctgctgcttc ttccactaat tttttcccga tcgcatgagg gcctttacga
    19921 aataattcag tcgtcccttt tccttctggc atttcctctt tgcgacgaca agcgagcgcg
    19981 tacatttctt caaaagtcat aagaatcctc tatgtttttt cactttacaa tatagaaaat
    20041 gcgcttattt cgctaaacat aaaagattct taccccatat aaaatgagtc gttttcattc
    20101 tcttttttct tgaatgcatt agctcagctc taaaatgatt acaagcttag cgtttttagt
    20161 tagtctctga ctattcttac acgaaataac gtttgaccat tttcgaaata cttacgttca
    20221 aaagccgttt cagggacact cggcacccac tcttctaaaa gcggagtcca tttcatttgt
    20281 aagtattgaa aaccaattcg agaagcctct aaaaactctt cggcataaat tttccaattc
    20341 gttcgaagct ccatgtttgg gcacaactgg agtaacgtag gaaaaatagg atgaccatgg
    20401 aatctacgcc cagcctgcga ttcttttggc caaggatttg gataatataa agcatgaaag
    20461 aaaacgttta actctgcctc taaagccaaa cgccaaaagt caattaattc tgcttgtata
    20521 taaaaaacat ttttgggttg actatttttt tgagctttgg ttaaacgtat ttgagactta
    20581 tcaataccta tcacggggtg atctggatac tttgcagcaa gcactaaagt actttctccg
    20641 cagccacaac cagaatctaa aactactggc ttttcaaaag tgcgaataaa atcttgagcc
    20701 tgattaaatg cttttatagt atgagaggca taaggccttt gataagttgt ttttaaatac
    20761 ctcaaaacct ttttttctaa atcagagaaa atttccgttt ggttggaaac gactcgcctt
    20821 accttattct cacttgacat caattaaaac caaaggtctt ctttagaaag tcctattaat
    20881 tctacgtcat ctaaagcaaa atcagatgat gaagtgaata cccaagaaac aacacgtatt
    20941 ccctttagta aagctggatt tgaagtaaag tcatttacag agaaagcaaa tcgcgtccat
    21001 tctgaaggca aatcaaacct ttgttcaggc catggcgtta ctacagacga atcttcttca
    21061 acaccaataa gttggaagaa cacagtccca gaacctttgg ccttaaaagc aacagaatct
    21121 aatcccgtca aatcatatac agaggagttc cctccgattt ggattccgaa attgacccaa
    21181 ggaacagaga ccgtctctgg cacagtcaca ttaaaagcaa tatactgaga ctcttctttt
    21241 actaaaggat aacccactaa taatgagtcc acaaataaaa catctacttc ttcatgagca
    21301 cttaaaaacc accagccagt agaatcacct acatatggtg caaaacgatg ttgggtattc
    21361 atatcttcaa aatcttctag caataaacgg tctgtttctt gcacaaaaga agaaggctca
    21421 attgaatctc caggcaataa aacggcataa gaatagtaag gagtcgactc acttccttga
    21481 gaaaagacta aagaagcact ccccgatgga agagaatcta aagagaaaag accattggca
    21541 tccactgggg cgcggtgacg cattccttga accgttacaa agcctgccga agcaaagcca
    21601 acagaacctt gtatttttga gaagcttctt aaagtatcga tacctcgatc aaaagaaaga
    21661 ctatcataaa ccactgcatt aaattgaaga gctcctgaag ggtccgagac ttctacggta
    21721 taagagccaa cggctaaatc ttgaacagaa aagcgacctt cgctatctgt cacagtagtc
    21781 ttcaaggttt gttcctctgt agagagaata gaactcgaag gcataataaa cacacgtgca
    21841 gcaaccgctg gtgttttatt tcctcttaag atttgagcgg tgattgcatt gcctgcttcg
    21901 gtccctgatg gaccacctcc agccacctct gaatggcatc ctaaaataaa aacaagtaag
    21961 cttcccaata aaaagagatt ccgcatcaag aaacctcctt aggatctgaa gacgctggat
    22021 tttctatcac atctgataag ggaaacatag aaacatttaa tgcataaacc ctgtttgcat
    22081 tttccgatct tttggcaaat tgaagtaatc cacgcctaaa atcttcaata cgctgtttaa
    22141 attcaggaat atctgcttca tcaacagtaa aaacaatcga agagacatct ctttctgcaa
    22201 cagaatgacg atctaaagac tcttgagcaa gctctaaggt acgcctatga aaatcacgaa
    22261 cggcacgact ttttacttct ccacccgtag tcacgatttg ttgggtaata ttccaacctc
    22321 catgtccatc gggctcaatt aattttaatt gttttaatac ctcaagagca ttatgtgcat
    22381 ctttctgaga aatcggagga ttcaaatggt tggcaagaac cgttaaatca gaggtgtctt
    22441 gactgatccc cactagagcc ctaagcgccg tacaagccca attgccaaaa tacataaatt
    22501 ctggacttgt taaaacgcga gcttccattt ctcggagttc cattaagcgg caataaagtt
    22561 cagataaaga agctttagct tttgttttac aaaatcgata taagattcta aaatattcag
    22621 ctcgccgatc atcaaacgaa cacaaacgaa taaaagcagg taaagcctct tcgtttaaat
    22681 gcccttcttt ttgaaaaaca cgaactagcc aagcagggtc aactcctgtt ttttgcccta
    22741 aataccgata agaagtaaaa cggttttcct ttttactttc tataaaccaa tctcttaaga
    22801 attctcggta ttctaaataa tctaggatat ctggcatact aataaattat actaaactta
    22861 aatcccttat cctattccta aagcaaaaag gagccctttc gttgttcttt gttcaacact
    22921 tctttcaaca taaatcgtga aataaatcac ttctcgtttg agattatcta tgttcttagt
    22981 atcctaatat cccaaaacct ataagctcta tgcagacccc tgcttagagc ttatactttt
    23041 ttagcccttt ttgacgtttt ttaacaaaaa aaggctcttg tttttagcaa gagccctttt
    23101 cagaaaaaat gttataggag cttcttatct tttataaaaa ccaaaaagtt ccacctaatt
    23161 gcaattgaag atttttagct tcaccaaaat cgtcactaac aagagaggtt aaacccattg
    23221 ctacacgaaa attcaagtct agcttaggca taacagtaaa accagcacca gcggcgagcc
    23281 ctaattcaaa tgtttcaaga acatcgattt catcagaacc taaatccatt gaaactgtag
    23341 caacatcacc aagttcagta acttctgttt ccatttcaga acttaatagg aaagcaaccg
    23401 taggaccgac ttcaaggaaa agctgtggca ttacatttac acgcaaaagt actgggatgt
    23461 taatagccat agttgtccaa gtcacgtcat catcagaggc acgacgcatt tcgaagtcaa
    23521 tttcaggggc taaatcaagc ataggaataa gagtgaattt tgctgcaaca ccagcattta
    23581 cgcctaggcc ccatgacacg tcttcattat attcaccaat agtggttcca aaattaagag
    23641 ctaagtgacc gcccacattt tgtgcaaaag aagttgttgc tagcattgca agaaccatga
    23701 atgacataag aattttgttc attatttaat ctcttttgat gtaaattaat aagataaaga
    23761 taactaaaaa aacgtttttt actttttaaa aaaaattgca tatcgctatg cgaccctcaa
    23821 gcattacgct ttgattagat gaatcccctt caaatgttct taaagttttt ataagtattt
    23881 caaaaaaaca ttccaagtat aaaaatcaga ttaataatcg ttgcccattt tttataaaaa
    23941 aagattaaaa aaaaagccct ctataggcat tgaatccttt gtatttaaaa agaaaaaagg
    24001 catcgccagc ggactttacg ctcgaagcta cttttttata aaagatagca gtgctttgca
    24061 aattgttttt cgtatcaaag atacgcctaa acaacattac aaaaacaacg agctgaacga
    24121 atcttgttag atcgggcata acaccgcggg atgccattaa taatataccc ttctttcaga
    24181 gaaaaaacaa gctcttcaaa tactacaatg aggaggattc ttcctctaac atactagcta
    24241 aaattcctgc tgttttttgg ctttcttgat agtaaatatg agcttttttt aaacgccgac
    24301 gagtatcaaa taattccgaa gcgatatcca tcgcgactaa aatcaattgt ttacgaattt
    24361 ctagacgcgg attactctta agatgttgat ccatcttttt tgtaacaaac cctacaattt
    24421 cttcaatttc cgcatcattt agatcggttt gtatctggaa tttttcttgg ccaaccgtta
    24481 cacgaacgga acggacttct tgatccgaag tgttcataag ctacctcgtt cctaaaccaa
    24541 aaggatcagt ggcttgtcgc cactttaaga gaaaaaatcg ctttgcggcg cctcctcttg
    24601 ctgttcattt ggaacagaag aagaatcttg ctctgaagga gcgatagaaa tttccgtgcc
    24661 aagttctgct tcaatagtac ttaacagctg ttgaaaacgt tcttgagcta attgaatttc
    24721 ttcctcttga tttttcactg tatcatttaa tgatgttaat tcaacactac gttcttccaa
    24781 actacttttc aacatggcaa tttcttcatc tttttgagcg agtacggaat caaaactttg
    24841 gatttttgac tccatttgag ctttttctgc ccttaaaaca cgaataactt caagcactct
    24901 ctctactttt tgtgtaagaa gatcaatatt ttccaaattc atttaaaact ccaggttaaa
    24961 agaatctttt ttatcatatt ctaatataaa atagttaaaa ataaagaagc catattattt
    25021 ttttatttag ctttatctca tctacattat gaaaactttt gcactagaag aacgtttatc
    25081 tcaactacca gcaaaaccag gcgtttatat tatgaaaaat gctgatggtg agattattta
    25141 cattggaaaa gcaaaagttt taaaaaatcg ggttcgtagt tattttgatg gacgagaaaa
    25201 aaatgggcac agggccgcca gtttaatgct tccccatatc catgatattg aatggattat
    25261 tacagaaact gaggcagaag ctttaattct cgaagccact ttaataaaaa aacatattcc
    25321 aatttataat gttcgcttaa aagatgacaa acattttccc tatttagttc tcactgtccg
    25381 cgaaccattt ccaaggctca ttttaactcg ttctattcgc tccgatggga acctttattt
    25441 ttgtcctttt ctaaatagtc gagccattca tcatttagtc gatattgccg ctcgtttgtt
    25501 tcatattaga gaatgtcatc taaagcttcc tggtaaagac gttattcgac cttgcttaaa
    25561 ctatcatatt gggcgctgtg cagctccctg tgcgggctta tgttctcaag aagaatacag
    25621 taaaggtgtt catgacgtta cgcttttact tgaagggaaa aggaacgatt taattagcga
    25681 atgggaacgt gaaatggaag aagccagtct taaactagac ttcgaaaccg cttctaaaaa
    25741 aagagatgcc attaacgctg taaaggcctt aaattctcgc caaaaagcag atgtttcaga
    25801 tgcctcgcta acacttgatg ttatttctgt aaaaaggcat cagaagcttt ctaccgcagt
    25861 gattttagaa tatcgtgcag gcgttttcta tggacgtcgc cattttaatt tagagtgcaa
    25921 tcttgagcaa agtgaatctg aaattttaga agaaatgatt ggttcttggt attttgataa
    25981 tgaagaaatt ccaaaagaaa ttgcgattga cacggaactt cctgaagatc atactctact
    26041 agaagaagcg ctttcagcaa aagcaaaaca taaggtttcg attatacgac cccagcgagg
    26101 agaaaaactg ggttttctta aactagcgca agcgaatgca gaaatgcttt tagtagaaat
    26161 gattgcttcc atccaaaaat atgatgatgt agagcaaagt gtttttactt tgcagcaaga
    26221 gcttcaatta aagaacaccc cttttcgtat tgagtgcgtt gatatttctc acctcgcagg
    26281 cacccacact gttgcgagct tagtatcttt taaaaatggt aaaccagata aatcaaatta
    26341 tcgcaaattt ataatcaaga gtgtttctgg agtcgatgat tttgcaagca tgagagaagt
    26401 cttaactcgt agaatcgagc ggttacataa ggaaaatatt cctatgcctg atttatttgt
    26461 tatggacggt ggaaaaggcc aagtggaagc gacggcaagt attcttagag aacttaatga
    26521 acccgatgtt cctttaattg gcttagctaa aagacttgaa gaaattgttt ttcctggaaa
    26581 tactccctct aaaatactac gccgacaaag ccccgctctt caattacttc aaaaagtccg
    26641 agatgaagct catcgttttg caattactta tcaaagatca aaaagaatct tagatctaaa
    26701 agtggaatgg ctttccttac ctggcatagg aacagccaca agaaaaaaga ttctatcaaa
    26761 atatcgcagt agagaggctt tcttaaaagc cccaaaagaa gatctagaga tccttttagg
    26821 taagaaaaaa agtctagtta tttacaaaac gataagtgaa tataaagaga aataaaagaa
    26881 aaacgctcca ttatgaaaaa atggagcgtt aagcaagttt agagaaagcg tttagttttt
    26941 cacacaacga acactaaagc cctttgtttt atagtctttt tggaagaata aagctggatc
    27001 tgcagcaaac tcagaatctg ggttcttttg gacaaagata aggctgcaat tggttgatcc
    27061 gtcacagtta tctttatctg cactatccat agttccagaa gcccaattaa agcccatacc
    27121 atcaaggaag ttataagaat tattactcat tcttccgtta tagttccaaa taaatgtagg
    27181 ctcttgcata gctgcttcaa actcagacct agtggcaaga tgccagccac ctggacaagc
    27241 taataaagca gcttcatgta catacaaacg cccataaaca tcacagtttg tttctttatc
    27301 ttcataacag aagctacctg atggcgtttc aaaaccaatg ttttgagcca tccaaatctt
    27361 atcgtttaag gtgatgattt cataagtttg attatctctt ttatcgacca tcactccatc
    27421 ttcaatcact ccatctccct gcaatataga agaggaggaa ggtataatca ccgaagaaga
    27481 gctgtttacc gaggaggaag aaggggcctc tgaagaagaa gacaagttat cctcacagcc
    27541 ttctgtgaag caaaatatac cgctgctgta gtagggatac tctacccatt gggaactgct
    27601 agattccttt ggtacagaac ttgaagaaga tttaatcact tcaccactac tagatgactt
    27661 acttgttgtt ccagtactac tggatgacgt tacttttatg gaagaagatg aggatgatga
    27721 aacaacctct gtagaagaag aggattttac atcatcttct tctgtttcgc tatcaaaaag
    27781 acaagcactt aaaaaaaggg agaaacttaa taataataag ctaaatactt tcattttcat
    27841 gcctataaaa atacattgtt attgaaaaat aactattgtt tatcaacttt tttactattt
    27901 ttacaatatg agtaacattg ctatttttga cactactttt gctgtgacag tactcacaat
    27961 aattgcactt ggagtaccat ttattcttct ttttgctaac tggctactac acccaggtcg
    28021 tattaaacgc actcttatta agggttccgc ttacgaatgt ggattagcgc acgtttcagg
    28081 aacagcaaac gatcgttatc ctgtaaaata ctatctagtc gccatgcttt ttcttgtatt
    28141 cgatttagaa gtagcttttc tttatccttg gaccattcag tttttgaaag ggggctggga
    28201 tctcttcttt atccttctag ggttcttgtt tatcctggaa ggcggctatt tttaccttta
    28261 taaaaaaggc gtattagatt gggctacatt aaaagactaa gtatttagaa cgcttcgcga
    28321 atataaatac tcattcccaa atgttttcca tcaattaaag aaaaatcaca gcgcgcgaaa
    28381 acgcgctctt ttttatttaa agctaaacgt cctccaaacc caaaagcatg gtgccatttt
    28441 tctctatata aagctccaaa ataagggccc acttttgttg tttcataaaa gatagtcccc
    28501 gctaggcgcc agaaaagagg acgacgtaat tcagcttgcc acacaaaagc ttgtcgatct
    28561 ctaaaaacac ctcgctccac gcctcggaaa cgtttagtgc catcaggcat cgctagttga
    28621 tcaaaaggaa cgtcaccaaa agtcatttga taaagagctc ctagagcaag agaggtcttc
    28681 cagaacaagt aagtgtaccc tcttaaatcg agctcttgaa aaatataaga agagtctcca
    28741 aaagcattat tgtaaaaaac ctgctgccaa taagcataaa atccgtgtga tggtaataca
    28801 ggatgatctc tagtatctaa agaaagtgta tagcccaaac ccgttagcca acctcct
//
LOCUS       DAOOBB010000004        28398 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_2177_length_28398_cov_4.833363, whole genome shotgun sequence.
ACCESSION   DAOOBB010000004 DAOOBB010000000
VERSION     DAOOBB010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 28398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28398
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2177_length_28398_cov_4.833363"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            551..1834
                     /locus_tag="PLT31_00460"
     CDS             551..1834
                     /locus_tag="PLT31_00460"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HPW93635.1"
                     /translation="MSNRVVIGSQWGDEGKAKVVDFLTLDADVIVRFQGGANAGHTVE
                     VEDRKFVFHLIPSGIMHQDKMCVIGNGVVLDPIQTLLEIEDLCQKGLDPKGRLMIANN
                     AHVVLPWHTAVDKLKELKAGKGAIGTTGRGIGPCYSDKVNRIGVRVGDLLDEKELRPR
                     IENMARIHNEEFKLMFGADPIDPETVIKEYLALGQQIAPYVKDVSEFLYNSLKDNKRL
                     IFEGAQGTILDVDQGTYPFVTSSNTVAGYASCGSGVGPKAIDQVFGVVKAYTTRVGNG
                     PFPTELLDEVGDELRKIGNEYGATTGRNRRCGWFDATVVRKAAVVNGLTHLAITKLDV
                     LDSFKEIQICTHYECDGEILNLIPNQLSKVGRCVPVYETMPGWESDTTKCRSWDELPL
                     AAKNYLKRLSELVDVKIGMVSVGARRDQTIIIDMN"
     gene            1845..2558
                     /locus_tag="PLT31_00465"
     CDS             1845..2558
                     /locus_tag="PLT31_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPW93636.1"
                     /translation="MNNADVAELLKGIDLFSKLTEAQLEQLISLVEPKDFCRDETIIL
                     EKDESVQALYLIASGEVKVYMTDSDGKETILSLLGRGDFFGEMSLIDGEPRSASVKAV
                     TAASLLVSRREHFIQLLRSTPELSISLLIEMCMRLRKANRHIGSLSTMSVFGRVGGTI
                     MNLIQEKGVRVHDGSGNMVTVIRNRPTQQQLAEMAGTTRETVSRIFSGLVKKGSISVA
                     GKDLIIYDENFLEDKDLKE"
     gene            2560..3315
                     /locus_tag="PLT31_00470"
     CDS             2560..3315
                     /locus_tag="PLT31_00470"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HPW93637.1"
                     /translation="MPIPNRFLNFIKKPFVQAFSIFLVIVFILVIVADSLVMPIIAGS
                     FTSETVVPDVLGLEKTRAEQILTDADFNFEWLAEGRYSSDVPAGHVLVQVPVPGRKAK
                     EGRTILLTKSKGIREVEIPDLRGKSQRQAEISLTRAGLIQGRIIKGAHLSIPRGVVIR
                     TNPSAGKLVRMGDTVNVVISAGETTGKKPLPQFEGLLLEEVFQKLEALGFVVGDVVRQ
                     KGADGISGTVLEQSPKSGDYLPAGTKIDFVIVD"
     gene            3324..5195
                     /locus_tag="PLT31_00475"
     CDS             3324..5195
                     /locus_tag="PLT31_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93638.1"
                     /translation="MRNKTWKALVLFFVLANVIACSSKTVQKQTVNETIQEKNDSVLV
                     KKTANLDIAHEAFLRALMLEQREEHGMALEFLAHASKADPENRYLAFQVAMKVAEMGD
                     YKMALALAEHAKNLKGPVKKSQLSLLASMYMQNRIVDSAKVYFKKAIDLDERDYLILY
                     EYSILLDAIKNYEELIRVYDLLLPQINYGKSLLNKQIDLLSAVGKDSALIDLLEQAYV
                     AHQDREYLEKKADLLIKNKRYDEAIAIADKFLSIAEKDTTGILIRSRALVLQGELNEA
                     LAYLKNAYFKLKIPSAMMLNFIALIELDLQEVDSARVHLKMLTENKTFAAPAHADLSS
                     LARADGDTLTAIAEMEKAAAIDPENYVANKAFLYYLYGRYQDAYQIYDSLLGYWSSWQ
                     PSPEILKKVKTSTAVAQMKLKANKKHQLVQTMYANALLHEAYALERKKDSVSTQKAKE
                     SRRQAGLFLESLLIADTNNISALFDLAANYERVGMVEKSINAFNLLLKKDPKHHQGLN
                     YLGYMLVDLNRNKNEVLRGASLIDSALKYLPKEPSYLDSKAWAFYRLGKYKDALHIME
                     LILSLQPKGIDDLVYWEHYVAITEKLGLNEKALEAYKQILSIDPNHPEAIKKLKKNQ"
     gene            5204..5950
                     /locus_tag="PLT31_00480"
     CDS             5204..5950
                     /locus_tag="PLT31_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93639.1"
                     /translation="MNQRLLLLFPLFVALFLSACASSKSLVPVEEKSKEFAADSLKAK
                     FTLSLKNEKGEMQELSGVLFAVQGIRYRLELSGPFGVGVASLLWNKEGWRITLPTEKL
                     YIEGQGYMIGDPSMPALPFVNIHQLAGIFYGDLLPASYKELSKKDSLDFEKVFAEDAL
                     GRPFQFVRKKASEEILSLMQMGVGGIAETIYYENPKEYEKKRIPSKIRFFRDQELFLE
                     VILKKVQHETTWGNGVWRLNIPQNYSKWNP"
     gene            6004..9384
                     /locus_tag="PLT31_00485"
     CDS             6004..9384
                     /locus_tag="PLT31_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93640.1"
                     /translation="MKKKILSLFLLLNAFAFSADTLEIFALRVEFQEEKTDNSLTTGT
                     GLFDSDPEAAKANFSLDPQGRRASSVYWKKHFEFADQYFRTVSGGQFVLKYRIFPESG
                     SAYKLDKFIIDYNRTNKRKDEKTAEYDEARSRDYMSFIWDAVDKAHQSSLSPFKIPLS
                     ENPNTKRAYMIIHAGASRLVDGGSMGTNGADTPGDFIDVFVSSDFWTYLPDSISQEKH
                     KNGIVFSDAAIDTLKEVMVVSETASQDGLNWGVNGIIVNQIGRFLGMPNTYDVVKGIS
                     RLGYFDVMDFAGYNAGNGFFPVLPSAWVRAYMGWATVQEVYPAAGKKLTFDLAAAGTG
                     LGTEIIKIPISASEYLLVENRQRSWNEDGTVEVLLSDAETEDAEVIKKKILVDSLSLV
                     FEDSVCVKGVCTVNKKKAEGIVVGINSYDAALPASGIVVWKVNEWYLKEALPYGVVNF
                     WGGDTLRDHQFGLSVIEADGVLSIGKTFKNALGQDTYDYGTGTDILPHVRYSKDTVRD
                     TVTTISSKGYANTASTTGGFSGLQIKAKPLSAYRQEKTENAFMGDSVLNFGSLIMKVE
                     LVFEDEKIPNSKFPKNTGVSSIPRGAVIVDYPSSVLKTGERAIAFGSADGTLQILSAF
                     GDSIMASDTVISMDGSIGIKNNKMQVPLYRLGSPVDSLLGLASKEHMIFSAHYSGLLK
                     TELVGLLDQVEYIQHFLPAKLKTGPLLLGDAIWAASNQKLYKADAKEFYPWVDSVSLP
                     ENFITHDMALCRDEENDKIVVVGADASVAIWSISEGKLWQGSLSQKKTNEFLSTKDQY
                     FRVTCSDFNRDGKSDLWILGSLGYGAMVTADSSFAPISLPHQYKRGFDEGGTRYHERS
                     SSAISDLNNDGYPDVVFTGFNKVYAVDHSGVPLDGFPATVSNGIPEYGFSSDPVVLDV
                     SGDASPDILIGTTSGLLMAFNNQGKRITSGFPLSAGSFEYGDTLYPMSIFAGELIDSL
                     SGPELYAFHRESVSGFRAFRASTGAEKNALAWSLPGHGNDRINWFDASLLKTPAKDAP
                     RETISDFYIYPNPIKGGHAKSRFTLGMDASSVWLEFYDITGLCVYKKKVESAEEGKNQ
                     VDYIDVSTLGSDIYSVRLKVKFVSGKEKQKNYRVGVIR"
     gene            9395..10249
                     /locus_tag="PLT31_00490"
     CDS             9395..10249
                     /locus_tag="PLT31_00490"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPW93641.1"
                     /translation="MKNTLWLLLCILLWACSNTTSPPEGVHYSQPFVIGISSPSVKSS
                     SSTTLPSESVTDIAFWESKWVQIPEQEMFVESKWIPIASFKILETEITQLMYAEVLDT
                     LPPQKELKENYPVASVSWYDAILFCNALSKNLGYDTAYRYSSIGPKNTLLNLTFVDSI
                     QSVRLPTNIEWEAAIRGTTTTEYFWGTGNASEFAHYGSTGGAIEVGQKRVNSYGLYDM
                     AGNVHEWVYDWDGPYGTTVGSKRIYRGGSWSSPLSQLASDYQSSALPDESTDTRGFRV
                     VLSNGVME"
     gene            10246..11172
                     /locus_tag="PLT31_00495"
     CDS             10246..11172
                     /locus_tag="PLT31_00495"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HPW93642.1"
                     /translation="MKSRSSRNKTIQLSTEEQTFFEKQIFKGSSYTVKNIENQVMQGD
                     SLNILSQLPGEFIDLVVTDPPYNMTKIYAGEKFSKTNADEYESWLRSWIEPLIPLLKP
                     NASLYVCTEWQCSSIVEKVLREYFYVRNRITWEREKGRGAKCNWKNVAEDIWFCTVSN
                     DYYFNADAVRLKRKVIAPYRHMNGDPKDWSDEGDEKFRLTAPSNLWTDISVPFWSMPE
                     NTEHPTQKPEKLIAKLILASSKEGDFVFDPFLGSGTTAVTAKKLSRRFAGIEKEKYYA
                     LLALKRLQMAEESFEIQGFKDGCFWERNTRPM"
     gene            11211..11651
                     /locus_tag="PLT31_00500"
     CDS             11211..11651
                     /locus_tag="PLT31_00500"
                     /inference="COORDINATES: protein motif:HMM:NF016087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein NlpE N-terminal
                     domain-containing protein"
                     /protein_id="HPW93643.1"
                     /translation="MLKKILFLLGLALLFTSCDGEKKNEQKLPPLKAIEIPKTISGLY
                     AGELPCDDCKKKQVKVQLDSLGEALVEEFFIQDSITKVLSKATYVDSAETVTLLFKET
                     NKKWVFKKEGSLSLVYLDLSGKPYYMESGIAYQMLKILNKPVKK"
     gene            11665..12438
                     /locus_tag="PLT31_00505"
     CDS             11665..12438
                     /locus_tag="PLT31_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HPW93644.1"
                     /translation="MIWKTLIVDDEPLARVRMRNLLLEYPQDIEIIGEAGSGEEAIQK
                     IRSLSPDLVFLDIQMPDINAFDVLNSLNEEEIPLVVFTTAFDDYALRAYEENTIDYLL
                     KPIEDERLSLTMEKLRKQAKAQVSTENDLLSSFSWDKFKQLVAAEQPFLSRIQVRIGD
                     RTILVNVDEVIRFQSEEKYTMAYTLTNQYLIDTPLVELEAKLNPQQFVRVHRAHLVAI
                     DYIAEIHRAEAGRLCIILKDKAKTMISVSRNFVKRVRHL"
     gene            12626..13981
                     /gene="lepB"
                     /locus_tag="PLT31_00510"
     CDS             12626..13981
                     /gene="lepB"
                     /locus_tag="PLT31_00510"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPW93645.1"
                     /translation="MDNQKKNVLQKTKQLFRGFAREILIPIVLALIVIQYVIQAFQIP
                     SGSMEDSLLTGDFLLGLKFTYGSPVPFTEKKFPGLTEPKPGDVVIFRYPGEPLYPDYD
                     RKRYSHIANALMFGNFYWDHKPLPGNPSLVHYPLGPKDFIKRCIAVSGDSIEVKGGQL
                     IYNGVPQTKLPGKGKYTSPYRSFSPRDELSLTKIPSPGDTIFLDSLPIEELWRIRSLM
                     VQENPQDHFELHLSLLEDGKPLSNYMFENFKVPVENDRNLLLNTLFSSRRVVSQQITY
                     GDTVTGPMPFAFFEKYALTGFLPRVDESIPVKGFKRVVDYEYFEGNQLKDLAHNVDLL
                     NQTIAVDSLDSLADTIPQVQKHYELRRVITKNDSVIKQYVVQEPVYFMMGDNRDNSAD
                     SRYWGFVGQQNIKAKAFVIYFSFENSDQKFSFSNPFSWWRIPFKIRWTRLGRIIHMIG
                     S"
     gene            13981..15756
                     /locus_tag="PLT31_00515"
     CDS             13981..15756
                     /locus_tag="PLT31_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPW93646.1"
                     /translation="MLVRKFIFLGCIVSLFSCATQVPPEGGPEDKLPPRIAAIAPAPN
                     SINQEPKLNVKILFDEWINATIPKSAITISPPIEKKMIFEVNGEELLISSRSVLDSNT
                     TYTLTVASGIKDLRGNSVAKPFELIFSTGSYIDSLSVSGRVMVSTEMLKKKQYPSIGL
                     FLIGKEREGKKYLQKYKDTTLTGVDSLPRLSKEPPLFITQADSNGWFSLGGLHAGRYR
                     VVSFVDVNGNRRIEPSVEWAGVYERDIELTETFKDSLWIALADQDTSLLELQQITQVG
                     KNLLQAKFSRFVHLDSFLQKKHNCFLTKNKVDTLFAEGAFKAPRQNDVQLYFEKAPSP
                     DSLYEFKCLYAVDSLARSLDTLRNSLELKWDAKLSDTLAPVVAETVPVTGSKLVFPNE
                     KIEVAFNKPVSGDTLASFLKLVINGDTGDVVLKRKDTVRFEISTASPWPTDGKVELLN
                     ISQDTVVKPADSTGYRDTVITPKFTTVVKFETVPKLKIASLEGKIPGTKRTLPVVRLR
                     SLETGKVLNIRTGPRGVFSIDNLLEGNYILDYYYNESTISNPDAGSLSPAKFGAGWRS
                     PSDTLILLNGKNKLDSLIQKLPSLP"
     gene            15769..18501
                     /locus_tag="PLT31_00520"
     CDS             15769..18501
                     /locus_tag="PLT31_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HPW93647.1"
                     /translation="MTLKIPSFVAIDLETTGLDFDKDEIIEVALVLFENGTPVKTSDF
                     LVKPKQDLRSFIEALTGITKEDLLSASDFADIAGQVRAFIGDYPIVAHNAQFDYKFLK
                     SAFSKVGINFESELVYDSLTISRIAFQDVPNHRLETLVNHLKIERTTAHRALPDAEAC
                     GNLFIKALETIDSFPAFEKKALFHVSKGSIWEAIFNEDASGTFDFVPGLVIDEKESVL
                     TNTSERKPRVAEFLEPDGFLSKTLPNFVSRENQIDYANVVERNMFKGGLSVLEAATGT
                     GKTIAYLLSAALKAVSGERVVISTATRALQEQLWQHELPKIEPLFEGKLKAMVLKGRK
                     NYICYRKFRDHLQSFDTLVSPEEKDSFMALIPWIQRSKTGDINENSGFNHLRNRSLWS
                     KLASDAGSCANESCPFYNRCPALEAKRKAMRSNLLFVNHALFLSDLALDFAILPAYEH
                     IVFDEAHRLPSLSHQAFARSVRFFDLRNIFKSLVHLKAEDKGLIADIENSLLKSKEQN
                     EEALALCSKLRTDIMESERLLHRFFMKIGKKVSKQKGSENTFKYKQSLFAELDADPKA
                     VLESLVAVKQTIAGLSSQLRQKEFALGLVRDLEGWGSDIEKVQYDLDFISSGNKDNWV
                     FYLEEPFNPHTLSMKAVPLDIGVFWADHFYKWIKSATFTSATLAVQGSLDYYAERMGM
                     TNSLPQKKRPFFRLYDAGSAFHSKRKIGIANFLPKPNAPEYQAELERFLAEILPEIEK
                     NVLVLFTSITSMLNVHKVLIPLFAQKNKLLLCQHADGTMDSLVDMFRKERKACLLGCQ
                     TMWEGVDLPGEALEVLIIPKLPFPNPSDPLISGKAEIIKEKGGNPFKTLFVPETYINL
                     SQGIGRLIRSEEDSGSIILLDNRLVSEPYGKTFLRIWNNEHKILHNPEEFKNAL"
     gene            18593..20458
                     /locus_tag="PLT31_00525"
     CDS             18593..20458
                     /locus_tag="PLT31_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93648.1"
                     /translation="MPKSLLRDVHAKAFGKKGLLNNTLIAKESIQFFSDKKRFEHSLS
                     LLESWQFNCLYLIYRSESRGLTYNELRLTIPVNKSRELKQFLLNASREYAIWQGKTTT
                     HVLLYKGFSDFVDCFENKGIEVYAPTETTGSLEYDYLFDWHICKILGMAAKGLLKVNL
                     TEGLHRRSFTLCEKAITYSKALSSKAPHDEILFILHFLSQNEWLGREEGQLKPTPKAN
                     QFLKKNGFRLRQDIVAWWIQQRFRGNEEHFKRLLEYLINPVNIVDAAYLFWVIDPSYR
                     ILERTKTLTWDYLPKPLRELWFLGFIDFKCARDKIHAVSLTKIGRAYLGNEVTPLVSG
                     DVSSLPNFELIIGAKNSPRILFFIATLTISQNDEPHLKCVFEKESFLDGLKSGFTEED
                     VEEFIHWIKPPKNVLDSLHDWSSCFFGAKIATVRLLKIENKKILEELMHFSHFMELVK
                     ESIPDYGFILDPKKEEGALKLLSHYGLLPNVNEKVISIEPFKNASWEKEFALHWPPNF
                     EPDYELKESLDKESFISSIEATKYGTLYRKLDLMDLIKVMRYAKSTNTMLAAQVKNPE
                     EKHADMKELFFYAQTLHFSKTPYVAKVQLKDTEDTIALDLNHIQEIKLVYQGIEA"
     gene            20811..22511
                     /locus_tag="PLT31_00530"
     CDS             20811..22511
                     /locus_tag="PLT31_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPW93649.1"
                     /translation="MRTFSTRIILFMLIAFNGLFAAVSPDFPIAGWAVSGNTTGGAGY
                     GEVTVDNTSDLKSYAKAGNKVIYVKPGTYAGQIDVGSNVTLYGYPGAIITQPHGQSAI
                     KVSGVSNVIIRNFIVKGIGAVDIDANDCVQINSGASKIWIDHLDVYDGQDGNLDIVNA
                     SDYVTISWTKFSYTSASSDHQFSNLIGNSDSKTTDSGHLRVTLHHNWWADGVKERMPR
                     VRFGRVHVANNLFETKTSSSCVRAGYKADLRVENNVFIGVKKPIDLYEGDYTAVSVSG
                     NYFEGTTGNTSGNKTAFTPSYSMPLTDVSNKAKAYALRDSIKNFAGATLPAPGTVYSS
                     SSVTPSSSSSLPPSSSSVFSSSSSLPESSSSSTIPASSSSVDVSAPAVLEKHGSGSSN
                     QTVAAGESIASFYYTWINASGVTVEGLPAGVSYVLDEEKKNVYFEGTVESSTPAGDYT
                     FTVTTVGNPITNAVKSGTIKVTSTTSIESSYFKSSGFQITPKIIQSEASITFNLKKQG
                     LVSLSIIDLSGREVFSKKWNASTGMNQMIVNSSSITAGVYYVQLKTPASESIQKIVIR
                     "
     gene            complement(22581..24293)
                     /locus_tag="PLT31_00535"
     CDS             complement(22581..24293)
                     /locus_tag="PLT31_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3416 domain-containing protein"
                     /protein_id="HPW93650.1"
                     /translation="MAIIPTKKENFVIENIRPSIEGGRFMIKREPGDKVTIQADIFRH
                     SHEKYDAAILYKHSSQKKWKQVPMEFVENDLWEGKFIVQNVGFYEYKIIAWTIAPEDI
                     PTESPVMKLRVDPKYARTGTWYEMWPKSQGNNPKASATWKDCENRLDYIDHLGFDTIY
                     LVPIHPIGVTNRKGANNALKAKKGEPGCPYAVGNSFGGHYAVDPELGTMEDFEHFVKE
                     CKKKGFRLALDIALNCSPDHPYVKEHPEWFFHEPDGSIKFAENPPKKYEDIYPFDYYN
                     ENFQALWEEIRDIILFWADKGIDIFRIDNPHTKPFPFWEWLISDIKEKRPEIVFLAEA
                     FTRPKIMHRLGKLGFDMSYTYFTWRSEKWEFEEYLKELTQSDAKEYMRGIFFPTTPDI
                     FPAYLADQGPNAFKQRYFLAATLSSLTGMYNGYELCENIKSPIKEELHDSEKYQYKVH
                     QWHEPGIKDFIRRVNLAREAHPAMQEYDNLRFHYAENTNLMVYSKKLEKDVILCIANM
                     DMHHAQEGTIALNMQELGLSEDAFFFVKDLITDESFVWRGSQNFVRLDPNKAPGHLFV
                     VKAI"
     gene            complement(24358..25647)
                     /locus_tag="PLT31_00540"
     CDS             complement(24358..25647)
                     /locus_tag="PLT31_00540"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HPW93651.1"
                     /translation="MSVYRFFQFCLIGLAIYLIGCAEKEPSAGIEIGNPSIAFTASFI
                     IDYGTQSDTQQSNNTLLAKMPSDNEALIIEDFCLHLTHIRSYSSYYVYVTIDPAEGLL
                     LWPYETMPEDTTLAVNFGNEIHAGESVIDEAFRKIDLQEEGILKEIGVGFKPNQNKPY
                     SISGTIKIGEEYIPFEYSLSAFEAIDLRYHYQQAEIYKDSLNLPVTFFVQRWINNIDF
                     SSARITNGVIRFSETENAALWTTLNERFLSSFSCLRWSWTSKDGDTISDYVSEALAQF
                     DKISTNWAVNGDFSNGSTGWILMEQYSGVADSTIIQESKNEFQMKVNVTKGGSKSYSV
                     QLIHEDIPVLQNRKYKLIFTAWSNIEGPITVRLGSYHTPYTTLGFQEKPIIGLNGKSY
                     EIEYLGVEDNTFARLEFNLGGAERIFWFKNIQLIRID"
     gene            complement(25647..26495)
                     /locus_tag="PLT31_00545"
     CDS             complement(25647..26495)
                     /locus_tag="PLT31_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02147 family protein"
                     /protein_id="HPW93652.1"
                     /translation="MIESADIKPKEPDILQYTNYRVFLRDYYEYKKKTSSAFSLRFFA
                     EKSGLSSHAHLKLAIDGKRNITKNTVVKLISGLNLDAKRAAYFESLVFFNQAKNDKEK
                     QIYYAQLLKASPRSKLRSLEKAQFRIFREWYHSVILEMTSLKGFNAAPEWISKHVMGG
                     VSPVQIAESLKILLDLGLLVKTANGFRQKDPLLTTDDEVQDILVKLYHNQMLKLASTA
                     LSELSGDQRDFSAVTFAIKRKDFPSLKKHLQLMRKELLDFSASNGEADDIVQVNIQLF
                     PLTRGV"
     gene            complement(26580..27134)
                     /locus_tag="PLT31_00550"
     CDS             complement(26580..27134)
                     /locus_tag="PLT31_00550"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPW93653.1"
                     /translation="MPIIEGNRIVLEPIEEKHAEIFLREVNSSRSFLDAKLPWVRAMN
                     TIEDALRFIDSYTLQSEMDNGGLWGLFEKENRTFLGCICLQWIQWDNLSASIGYWLLK
                     SHTGKGYATEALKMICSYAFNSLKLNRLEIDIAANNLKSIALVKRVGFQEEGFFREYA
                     NLHGIFLNHLRFSLLSTDPSHLKL"
     gene            complement(27134..27454)
                     /locus_tag="PLT31_00555"
     CDS             complement(27134..27454)
                     /locus_tag="PLT31_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93654.1"
                     /translation="MRKIPAEGATARSCYTLTGDITYQYDQNLCQRKPCFFLYEIDSK
                     EKRLNTFIYLSDSLNSGILPNWKDTSNHEITRITGYYYPNRIPSTTPLTPDSQEAFFV
                     MDIE"
     gene            complement(27599..27682)
                     /locus_tag="PLT31_00560"
     tRNA            complement(27599..27682)
                     /locus_tag="PLT31_00560"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27646..27648),aa:Leu,seq:cag)
     gene            complement(27719..>28398)
                     /locus_tag="PLT31_00565"
     CDS             complement(27719..>28398)
                     /locus_tag="PLT31_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93655.1"
                     /translation="LTQVFALKDSLIIMEKDFNSPAGVWFSTPKKQNSPSVIIWFHGG
                     MQSSKCQKGLVAGQGLFELLEDPNVIIASPSACLDKHWLSPTLIKITDKLIDSLEHHF
                     NILIKEVNLVGVSDGALGVFAYSMNGKRAILSRLLISSNLSIVGDPKVFAQNTRLRSG
                     AWFFMQGGQDRLYPASHVLPWLNSFCESIGSSCNVFFDSKGEHDWSYWTQKHPKRIQQ
                     FKHPKLP"
BASE COUNT         8625 a         4841 c         5861 g         9071 t
ORIGIN      
        1 ctctaaaagt cgagatattt gatttcaaag gcaatcttat gaagtctagt cagatgaaat
       61 caaataacct acttattaat ctaaaaggaa ttccatctac agttcttttg gtttctgtta
      121 caaaaggcag cgaaagacag gtttttaagg ttattcgaaa ataattaatg gttcatcgcc
      181 ataaaaaggc tttgcttttt tttatcaata ccatactgat ttcgaatcca cattatagac
      241 aaaaatgttt tttagattcc ctttcaattt aaatagcaat gatggacggc aagtttgctt
      301 gcttttgcca aaaaatacga aaagtaccat tttgatgcat ttgaagggaa gtctaaacct
      361 ttctttacag aggctttttt aaaaaatgtc ttaaaccctt caaaaaacgt taatttcttg
      421 cttttagctg taaaacaagt tcatctcttt ttttaatacc tcatttgacc tctttttttc
      481 tactttttag acgaaaaagg actacaagga aggaccttgc tggtttttgt tttttttgga
      541 tgaaggtttt atgtcgaatc gagttgttat tggctcccaa tggggcgacg aaggtaaagc
      601 gaaagtagtt gattttttaa ccttagatgc ggatgtgatt gtccgttttc aaggtggtgc
      661 taacgctggg catactgttg aagtagaaga tcgaaagttt gtatttcatt taatcccatc
      721 tgggataatg catcaagata agatgtgtgt tattggaaat ggtgttgttc ttgatccgat
      781 tcaaacattg cttgaaattg aagatttatg tcaaaagggc ttagatccaa aaggccgttt
      841 aatgattgca aacaatgctc atgtggtttt accttggcat acagctgtcg ataagcttaa
      901 agaattaaag gctggtaaag gtgcgattgg aacgactggt cgtggtattg ggccttgtta
      961 tagtgataag gtaaatcgta ttggtgttcg cgtgggcgat ttattagacg aaaaggaact
     1021 tcgtcctcgt attgagaaca tggctcgaat tcataatgaa gaattcaaat tgatgtttgg
     1081 agccgatcca attgatccag aaacagtgat taaagaatat ttagctttgg gtcagcagat
     1141 tgctccttat gtgaaggatg tgagtgagtt tttatataat agccttaagg acaataagcg
     1201 cttgattttt gaaggggctc aaggaacgat tcttgatgtg gatcaaggaa cttatccttt
     1261 tgtgacttca agtaacacag tcgctggcta tgctagttgt ggttctggtg ttggtccaaa
     1321 agcaatagat caagtttttg gtgtggtgaa agcttatact acacgtgtag gtaatgggcc
     1381 gttccctacc gaacttttag atgaagtcgg agacgaatta cggaaaattg gtaatgagta
     1441 tggtgctacg actggccgta atcgtcgctg tggttggttt gatgcgactg tcgtgcgtaa
     1501 ggctgcggtg gtgaatggct taactcattt agcgattacg aaattagatg ttctcgattc
     1561 ttttaaggaa attcaaattt gtactcacta tgaatgtgat ggtgagattt tgaatttgat
     1621 tccaaatcag ctttctaagg taggtcgttg cgttcctgtg tatgaaacca tgcctggttg
     1681 ggagtctgat acaacgaagt gtcgctcgtg ggacgaattg cctttggcag caaaaaatta
     1741 tttgaaacga ctttctgaat tggtcgatgt taaaattggg atggtgtctg ttggagctcg
     1801 tcgtgatcaa acgattatca tagatatgaa ctgaggagaa gctaatgaat aatgcagatg
     1861 tcgctgaatt actcaaggga atagacctat tttcaaagtt aacggaagca cagttagaac
     1921 aactgatttc gttagtggag cctaaagatt tttgtcgaga tgaaacaatc attctcgaaa
     1981 aagatgaatc ggttcaggca ctatacttga tcgcttctgg tgaagtgaag gtgtatatga
     2041 ctgattcaga tgggaaagag actattcttt ctctgttagg ccgaggtgat ttttttggcg
     2101 aaatgtcttt gattgatggg gaacctcgtt ctgcttcggt gaaggctgtc acagctgcct
     2161 ctttattagt gagccgtaga gaacacttca tacaattact acgctctact ccagaactat
     2221 ccatttctct tttgattgaa atgtgtatga gactacgtaa agcgaatcgt catatcggtt
     2281 ctctttctac gatgtccgtt tttggccgtg taggcggtac gattatgaat ttgatccaag
     2341 aaaagggcgt tcgagttcat gacggtagtg gaaatatggt aactgtgatt cgtaatcgcc
     2401 caacgcaaca acaacttgct gaaatggctg gtacaacgag agaaactgta agtcgtattt
     2461 tttcaggttt agtgaagaaa ggatcgattt ctgtcgctgg caaagattta attatttacg
     2521 atgaaaattt cttagaagac aaggacttaa aggagtaaaa tgcccattcc aaatcgtttt
     2581 ttaaatttta taaaaaaacc ttttgttcag gctttttcaa tttttttagt cattgttttc
     2641 attttggtta ttgttgctga ttctttagtc atgcctatta ttgctggctc ttttacttca
     2701 gaaacggttg ttcccgatgt tcttggctta gaaaaaacaa gagcagaaca aattttaacc
     2761 gatgccgatt tcaattttga atggcttgcc gaagggcgtt acagtagtga tgtacctgcg
     2821 ggtcatgttc ttgttcaagt accagtacct ggtcgtaagg ctaaagaagg aagaacgatt
     2881 cttttaacaa aaagtaaagg aattcgcgaa gtagaaattc ctgatctacg aggcaagagt
     2941 caacgtcaag cagaaatttc tttgactcga gcaggcttaa ttcaaggacg tatcatcaag
     3001 ggagctcatc tcagtatacc tcgtggtgtt gtgattcgta cgaatccttc tgcaggaaaa
     3061 ttagtccgta tgggagacac cgtgaatgtg gttatttctg cgggtgaaac tacagggaaa
     3121 aagccattac ctcaatttga aggcttgctt ttagaagagg tttttcagaa attagaagca
     3181 ttaggctttg ttgtgggtga tgttgttagg cagaaaggcg ctgatgggat ctctgggact
     3241 gtattagaac aatctccgaa atcgggtgac tatttgccag cagggacaaa aattgatttc
     3301 gtaatcgttg attaagggcg catatgcgca ataaaacttg gaaagctctg gttctttttt
     3361 ttgtactagc aaacgttatc gcttgtagta gtaagactgt tcaaaaacag acagtgaacg
     3421 agacgattca agaaaaaaat gactccgttt tagtaaaaaa aacagcaaat ttagatatag
     3481 ctcatgaagc ttttctaaga gctcttatgc ttgaacaaag agaagagcat ggcatggctc
     3541 ttgagttttt agcacatgca agcaaagcgg atcctgaaaa tcgatattta gcttttcaag
     3601 tggcaatgaa agtggctgaa atgggtgatt ataaaatggc attagcatta gcagaacatg
     3661 ccaaaaactt aaaaggccca gtaaaaaaat cgcaattaag tcttttagct tcgatgtaca
     3721 tgcaaaatag aattgtagat tcagcaaaag tctattttaa gaaagctatc gatttagatg
     3781 aacgggatta tttaatcctt tatgaataca gtattctttt ggacgcgata aaaaactatg
     3841 aagaattgat tcgtgtgtat gatcttttgt tgcctcaaat taattacggt aaatcgcttt
     3901 tgaataaaca gattgattta ctctctgctg tgggtaaaga ttctgctttg attgatttgt
     3961 tggaacaagc ttacgtggct catcaagaca gagaatatct tgaaaaaaaa gccgatttat
     4021 taatcaaaaa taaacgctat gatgaggcga ttgctatagc cgataaattt ttatccatag
     4081 cagaaaaaga tacaacagga atattgattc gttctcgtgc tcttgtttta caaggcgaat
     4141 tgaacgaggc tcttgcttat ttaaagaatg cttattttaa gctaaaaatt ccatctgcga
     4201 tgatgctcaa ttttattgct ttgatagagc ttgatttgca agaagtagat agcgctagag
     4261 tacacttaaa aatgttgacc gaaaacaaaa cttttgctgc tcctgctcat gcagacctga
     4321 gttctttggc aagagccgat ggcgatacgt taacggccat tgctgaaatg gaaaaagccg
     4381 cggcaattga tcctgagaat tatgtggcaa ataaagcatt tttatattac ttgtacggtc
     4441 gctatcaaga tgcatatcag atttatgatt ctttgctagg ctattggagc tcttggcaac
     4501 cttcacctga aatattgaag aaggtaaaaa catcaacagc cgtcgcacaa atgaaattga
     4561 aagctaataa gaagcaccaa ttggtgcaaa cgatgtatgc gaatgcttta cttcatgaag
     4621 cctatgctct agagaggaaa aaggattccg tttctactca aaaagccaaa gaatctcgta
     4681 ggcaagccgg tctcttttta gaaagtcttt tgattgccga tactaataat atctctgccc
     4741 tttttgattt ggctgcaaat tatgaacgag tgggaatggt tgaaaaatcg ataaacgctt
     4801 ttaatttact tcttaaaaaa gatccaaagc atcatcaggg tttaaattac ttagggtata
     4861 tgcttgtgga tctaaatcgc aataaaaatg aggttttgcg aggagcctct ttgatcgatt
     4921 cggctttaaa gtatttacca aaagagccct cttatttaga ttcaaaagct tgggcttttt
     4981 atcgcttagg aaaatataaa gacgcgcttc atattatgga attgattctt tctctgcaac
     5041 caaaagggat tgatgatttg gtttattggg aacattatgt ggcgatcact gaaaagttag
     5101 ggttaaatga aaaggcttta gaagcttaca agcaaattct atctattgat ccaaatcatc
     5161 cggaagccat aaagaaactc aagaaaaatc agtagggcaa agaatgaatc aaagattgtt
     5221 gcttctcttc cccttatttg tggccttatt tttgtccgct tgtgcctctt caaaatcact
     5281 agttcctgtt gaagaaaaat caaaagaatt tgctgccgat agcttaaagg caaaattcac
     5341 tttatctcta aaaaatgaaa aaggcgaaat gcaagagtta tctggtgtgt tatttgccgt
     5401 tcaagggatt cgttatcgtc tagaattgag tggccctttt ggggtgggag tcgcttctct
     5461 attatggaac aaagaaggct ggcggatcac tttacctaca gaaaagttat acatagaagg
     5521 gcaagggtat atgattggag acccttcgat gcctgcatta ccttttgtga atatacatca
     5581 actcgcaggt attttttatg gtgatttatt gcccgcttct tataaagagt taagtaaaaa
     5641 agattcctta gattttgaaa aagtatttgc tgaagatgct ttagggcgtc cattccagtt
     5701 tgtgcgaaaa aaagcttcag aagaaatttt gtctttgatg caaatggggg tcggtggaat
     5761 tgcggaaacg atttattatg aaaaccccaa ggagtatgaa aaaaagagga tcccgtctaa
     5821 gattcgtttc tttcgagatc aagaattgtt cttagaagtg atactgaaaa aggttcagca
     5881 tgaaacgact tggggaaatg gcgtttggcg tctcaatata ccccaaaatt acagtaaatg
     5941 gaatccttaa cagagtgctt gtttagtaga ttaatagaag agtgtataaa acccgaatag
     6001 attatgaaaa aaaagatatt gtcattattt ttattgttga atgcgtttgc tttttctgca
     6061 gacacgcttg aaatttttgc gttgcgcgtg gaatttcaag aagaaaaaac agacaactca
     6121 ttaacaactg gaacaggcct tttcgattct gatccagagg ccgcaaaagc taattttagt
     6181 ctagatccac aagggcgtcg ggctagttct gtatactgga aaaagcactt tgagtttgct
     6241 gaccaatatt ttcggacggt gagcggaggg caattcgttc tgaagtaccg tattttccct
     6301 gagagtggtt ctgcttataa actagataag tttattattg attataatcg cacgaataaa
     6361 agaaaagatg aaaagacggc tgaatatgat gaggctcgaa gtcgggatta tatgagtttt
     6421 atatgggatg ccgtagataa agcgcatcaa tcatcattgt ctccgtttaa gattcctttg
     6481 tcagaaaatc caaacaccaa acgggcgtac atgattatcc atgcaggtgc aagtcgcctt
     6541 gtggatggtg gttctatggg aacaaatggg gccgatactc caggtgattt catcgatgtt
     6601 tttgtctctt ctgatttctg gacatattta cctgattcca tttcacaaga aaagcataaa
     6661 aatggaattg tattttctga tgctgcgatt gacaccttaa aagaagtgat ggtagtgagt
     6721 gaaacggcgt ctcaagatgg attgaattgg ggtgtgaatg gcatcatcgt gaatcagatt
     6781 gggcgctttt taggcatgcc taatacttat gatgtcgtaa aaggtatttc tcgcttaggt
     6841 tattttgacg tgatggactt tgctggttac aatgctggga acggcttttt tccagtactt
     6901 ccaagtgctt gggtgcgagc ctacatgggc tgggcaactg ttcaagaagt ctaccctgca
     6961 gcagggaaaa aattaacttt tgatttagct gcagcaggga cagggctcgg caccgaaatt
     7021 ataaaaattc caataagtgc ttcagaatat cttttggttg agaaccgcca acgttcttgg
     7081 aatgaagacg gcactgtcga agttttactt tcagatgctg aaacagaaga tgctgaagtg
     7141 attaagaaaa aaatcctggt tgatagtctt agtttagttt ttgaagatag cgtttgcgtg
     7201 aaaggcgttt gtactgtaaa taagaaaaaa gcagaaggaa ttgtcgttgg gattaattct
     7261 tatgacgccg ctcttcctgc gagtggaatt gtggtttgga aagtgaatga atggtatctt
     7321 aaagaagcgt taccatatgg ggtggttaat ttctggggtg gagatactct tcgagatcat
     7381 caatttggct tgtctgtgat tgaagccgat ggtgtgttaa gcataggtaa aacatttaaa
     7441 aatgctttag ggcaagatac ttatgattat ggcactggaa cagatatttt gcctcatgtc
     7501 cgttacagta aggatactgt tcgagatacg gtgactacca tttcttctaa gggctatgct
     7561 aatacggcct caacaacggg aggcttttct ggcttacaga ttaaggcaaa gccactttct
     7621 gcgtatcgtc aggaaaaaac agaaaatgcc tttatgggtg atagtgtttt aaattttggc
     7681 tctttaatta tgaaggtaga gcttgttttt gaagatgaaa aaattccaaa ttcaaagttt
     7741 cctaaaaaca cgggcgtctc ttcaattcct cgtggagcag ttattgtaga ctatccttct
     7801 tctgtattaa aaacagggga gcgtgcgatt gcttttggaa gtgccgatgg tacgttacaa
     7861 atcctttctg cttttggtga ttctatcatg gcttcggata cagtgatttc tatggatgga
     7921 tcgataggca taaagaataa taaaatgcaa gtgcctttat atcgattagg gtcccctgta
     7981 gattctttat taggattagc ttctaaagaa catatgattt tttcagcaca ttattctggc
     8041 ctgttgaaaa cagaattagt ggggctttta gatcaagtgg aatatattca acactttttg
     8101 cctgcaaaac tcaaaacagg accgttgtta cttggtgatg ctatttgggc cgcgtccaat
     8161 cagaaacttt ataaagcgga tgcaaaagaa ttttatcctt gggtggattc agtttctttg
     8221 cctgaaaatt ttattactca tgatatggct ctttgtcgtg atgaagaaaa tgataagatt
     8281 gtggtggtcg gagccgatgc ttctgtagcc atttggtcta tctcagaagg aaagctttgg
     8341 cagggttctc tgagtcaaaa gaaaacgaat gagttcttga gtacaaaaga tcagtatttt
     8401 agagtgactt gctccgactt taatcgagat gggaaatcag atttgtggat tttaggaagt
     8461 ttaggttatg gagcaatggt taccgcagac tcttcttttg ctcctattag cttacctcat
     8521 caatataaaa gaggttttga tgaaggtgga actcgttatc acgagcgctc ttcttcggca
     8581 atttccgatc tgaataatga tggttatcca gatgttgttt tcactggttt taataaagta
     8641 tatgctgtag accattctgg agttcctctc gatgggttcc ctgctacggt ttcaaatgga
     8701 atccctgaat atggattctc ttcggatcca gtcgttttag atgtttcagg cgacgcttct
     8761 ccagatattc ttattggaac gactagtgga ttattgatgg catttaataa tcaaggaaag
     8821 cgaatcacaa gtggattccc tttgtctgct ggctcttttg aatatggcga tacattatac
     8881 ccgatgtcta tttttgcagg agaactgatt gattctttaa gtggccctga gctatacgct
     8941 ttccatcgtg aatcggtttc tggctttaga gctttccgcg cttctacagg ggccgaaaaa
     9001 aatgcacttg cttggagcct tcctgggcat ggaaatgatc gaatcaattg gtttgatgca
     9061 agcttgttaa agactccagc gaaggatgct ccaagagaga cgattagcga tttttatatc
     9121 tatcctaacc ctattaaagg aggccatgcg aagtcgagat ttacgttagg catggatgct
     9181 tcttctgttt ggcttgaatt ttacgatata actgggcttt gtgtatataa gaagaaagtg
     9241 gaaagtgccg aagaaggaaa aaatcaagta gattatatag atgtatctac tttgggttcc
     9301 gatatttatt ctgtgcgatt gaaagtgaaa tttgtttctg gtaaggaaaa acaaaaaaat
     9361 tatcgagttg gagtaatccg ctagaaaaaa gaatatgaaa aacacactct ggttactttt
     9421 gtgtattctt ttatgggcct gttcgaatac gacaagcccc ccagaagggg ttcattattc
     9481 tcaaccattt gtgataggta tttcttctcc aagcgtaaaa tcctcctcct ctactacatt
     9541 gccttctgaa agtgtaacgg atatcgcttt ctgggaatca aaatgggttc agattccaga
     9601 gcaagaaatg tttgtggagt cgaaatggat tccgattgct tcttttaaaa ttttagaaac
     9661 agaaattact caattgatgt atgcagaagt tttagatact ttaccgcctc aaaaagagtt
     9721 aaaagaaaat tatcctgtag cctctgtttc ttggtatgac gccattcttt tttgtaatgc
     9781 cttatcaaaa aatttaggct atgatacggc ttatcggtat tcgagtattg ggcctaaaaa
     9841 tacgttgctg aatttaactt tcgtggattc gattcaaagc gtgcgcttac ctacgaacat
     9901 tgagtgggaa gctgccatac gcggaactac aaccacggaa tacttttggg gtacaggaaa
     9961 tgcctctgaa tttgcacatt atggaagtac tggtggggct attgaagtag gccaaaaaag
    10021 agtaaactcc tatggtttgt atgatatggc tgggaatgtg catgaatggg tctatgattg
    10081 ggatggacct tatggtacaa ccgtgggctc gaaacgcatt tatagagggg gctcttggag
    10141 tagcccttta tctcaattgg cttctgatta tcaaagcagt gcattgccag atgagtctac
    10201 agacactcgt ggatttagag tggtattatc gaatggagtg atggagtgaa gtctaggtca
    10261 tcgcggaata agacaattca attaagtaca gaagaacaaa ctttttttga aaaacagata
    10321 tttaaggggt cttcttatac cgttaaaaat attgaaaatc aggtgatgca gggtgattcc
    10381 cttaacatct tatctcaatt accaggagag tttatagatc ttgttgtcac tgatcctccc
    10441 tacaacatga ctaaaattta tgcgggtgaa aagttttcta agacaaatgc ggacgaatat
    10501 gaatcttggc ttcgctcttg gattgaaccc ttgattcctt tactaaagcc caatgcttca
    10561 ctttatgtat gcaccgaatg gcaatgttcg agcatcgttg aaaaggtgct tcgagagtac
    10621 ttttatgtgc gaaatcgaat tacttgggag cgagaaaaag gtcgtggcgc aaaatgcaac
    10681 tggaaaaatg ttgccgaaga tatttggttt tgtacggtct ctaatgatta ttattttaat
    10741 gcagatgctg ttcgattaaa aagaaaggtg attgctcctt atagacatat gaatggagat
    10801 cctaaagatt ggtctgacga aggtgatgaa aaattccgtt tgacagctcc ctctaatttg
    10861 tggacggata tttcggttcc attttggtcg atgccagaga atacggaaca cccgacgcaa
    10921 aagccagaga agcttattgc gaaactgatt ttagcgagtt cgaaggaagg cgacttcgtc
    10981 ttcgatcctt tcttgggctc tggtacgaca gcggtcaccg caaaaaaatt atctcgtcgt
    11041 tttgcaggga tagaaaaaga aaagtattat gcccttttag ccttaaaacg attacaaatg
    11101 gcagaagaat cttttgaaat tcaaggcttt aaggatggct gtttttggga acggaacacg
    11161 cgacctatgt agttgatgtt tttagctcta ttttttacta atttgttgtg atgttgaaaa
    11221 aaatattatt tctcttgggt ttggctcttt tgtttacgtc ttgcgacgga gaaaaaaaga
    11281 atgaacaaaa attaccacca ctaaaggcaa tagaaatccc aaagacgatt tcaggtcttt
    11341 atgcgggaga attgccttgt gatgattgta agaaaaaaca agtgaaagtg cagctcgatt
    11401 ctttagggga agctttagta gaagagtttt ttattcagga ttctataact aaggttttat
    11461 caaaagcaac atatgttgat tcggcagaaa ctgtgacttt gctttttaag gaaacgaata
    11521 aaaaatgggt cttcaaaaaa gagggctctt tatctttagt gtatttggat ttatcaggaa
    11581 aaccatatta tatggaatca ggcattgcat atcagatgct taagattttg aataagccag
    11641 tcaaaaagtg atgtaggaat aaaaatgatt tggaaaacgt taattgttga tgatgaacct
    11701 ttagctcgtg tgcgtatgag aaacttgctt ttggagtatc cgcaggatat tgaaatcatt
    11761 ggcgaagcag gttcaggaga agaggccatt caaaaaataa gatcgctttc tcccgactta
    11821 gtttttttag atattcaaat gcctgatatc aacgcttttg atgtattgaa ctctttaaat
    11881 gaagaagaaa ttcctttagt tgtttttaca acggcttttg atgattatgc tcttagggct
    11941 tatgaagaaa atacgatcga ttatctttta aaaccgatcg aagatgaacg attgagttta
    12001 accatggaaa aactccgtaa gcaggcaaag gctcaagtct ccacagaaaa tgatcttttg
    12061 agttcttttt cttgggataa gtttaaacaa ctcgtcgctg cagaacaacc ttttctatca
    12121 cgtatacaag ttcgaattgg agatagaacc attctagtaa atgtcgatga agtgattcgc
    12181 tttcagagtg aagaaaaata tacgatggct tatactctca ccaatcaata tttgattgat
    12241 acccctcttg tagaattaga agcaaagttg aatcctcagc aattcgtgcg cgtgcacagg
    12301 gctcatttag tggcgattga ttatatcgcc gaaattcata gagcggaagc gggtcgcctg
    12361 tgtattattt tgaaagataa agcgaagacc atgatttctg tgagtcgaaa ctttgtgaag
    12421 cgtgtacgtc atctttaagc tattgttttt tatggaatcc ccaaaagtat atcgcctcaa
    12481 ttcgttatat aaattaatga atacagccag tatttttcaa tatgtaattc tgcttctgct
    12541 cgactttatt ctaatttttg atcgtaatgt cgttttaaga ggtgagtgtt agtttcactg
    12601 tttaaatttt tctaatttgg aatttatgga taatcaaaag aaaaacgttc ttcaaaaaac
    12661 aaagcaactg tttcgtggtt ttgctcgtga aatacttatt cccatagtac ttgctttaat
    12721 tgttattcaa tatgtgattc aagcttttca aatccctagt ggctcgatgg aagattctct
    12781 tttaactggt gattttcttt tgggattaaa atttacttat ggctctcctg taccttttac
    12841 agaaaagaaa tttcctggat taacagaacc aaaacctgga gatgtcgtga ttttccgcta
    12901 cccaggagaa ccattgtatc ctgattacga tcggaaacga tattctcata tagcgaatgc
    12961 tttaatgttt ggaaatttct actgggatca taaaccgctt cctggaaatc caagccttgt
    13021 gcattatcct ttaggcccta aagattttat taaacgctgt attgcggtga gtggtgattc
    13081 tatagaagtc aagggcgggc aattgattta taatggagtt ccacaaacga aactgccagg
    13141 caaggggaaa tacacttctc cttatcgttc gttttctcca agagatgaat taagcttaac
    13201 aaaaattcca agtccaggag atactatttt cttagactct cttccgattg aagaattgtg
    13261 gaggattcgt tctttgatgg ttcaagaaaa tccacaggat cattttgaat tgcacttgag
    13321 tttactcgaa gatggaaaac ctttatcgaa ttatatgttt gaaaacttta aggtacctgt
    13381 agaaaatgat cgtaatttgc ttttgaatac gctcttttca agccgccgtg tagtgtctca
    13441 acaaataact tacggcgata cagtaacagg tcctatgcct tttgctttct ttgaaaaata
    13501 cgccctcact ggatttttac caagagtcga tgaatcgatt cctgttaagg gttttaagcg
    13561 tgtcgtagat tatgaatact ttgaagggaa tcaattaaaa gatttagctc ataacgtaga
    13621 tctcttaaac caaacaattg ctgtagattc tttagattca cttgcagaca ctattcctca
    13681 agttcaaaag cattatgaat tacgtcgagt cattacgaaa aatgattctg tgatcaaaca
    13741 atacgtagtt caagaaccag tttattttat gatgggtgat aatcgagata attctgcaga
    13801 tagcaggtat tgggggtttg tcggtcagca aaatattaaa gcaaaagctt ttgtgattta
    13861 tttttcattt gaaaatagtg accagaaatt ctcattctct aatcctttct cttggtggcg
    13921 gattccattt aagattcgtt ggactcgtct tggaagaatt attcacatga ttggttcgtg
    13981 atgcttgtac gcaaattcat cttcttaggt tgtatcgtct ctcttttctc ttgtgctaca
    14041 caagtgcctc cagagggtgg ccctgaagat aaattgcctc caagaattgc agccattgcc
    14101 cctgctccaa attcgattaa tcaagaaccc aaattaaatg tgaaaattct ttttgatgaa
    14161 tggattaacg cgacgattcc aaaaagtgcc atcactattt ctcctccgat tgaaaagaaa
    14221 atgatttttg aagtgaatgg ggaagaactt ttaatttctt ctcgttctgt tctagattca
    14281 aataccacat atactttgac ggtggccagt gggattaaag accttcgtgg aaattctgta
    14341 gcgaagcctt ttgaacttat cttttctaca ggttcttaca tagattctct gtctgtttct
    14401 gggcgagtca tggtgtctac agagatgctc aagaaaaaac aatacccaag tattggtctc
    14461 tttttgattg gaaaagaacg tgaagggaaa aagtatcttc aaaagtataa agacaccact
    14521 ttgactgggg tagattcgct tcctcgttta tctaaagaac ccccgctgtt tatcacacaa
    14581 gcagatagta atggctggtt ttctcttggt ggcttacatg ctggtcgtta tcgagtggta
    14641 tcgtttgttg atgtgaatgg aaatcgtcgc atagagcctt ctgtagaatg ggctggtgtt
    14701 tatgaacgag atattgaact cacagaaacg tttaaggatt cgctttggat tgctttagca
    14761 gatcaagata cttctttatt agaactccaa caaattactc aagtgggtaa aaatctgttg
    14821 caagcgaaat tttcaagatt tgtgcactta gattctttcc ttcaaaaaaa acataattgt
    14881 tttttaacaa aaaataaggt agacacttta tttgcagaag gtgcttttaa agcccccagg
    14941 caaaacgatg ttcagcttta ttttgaaaaa gctccatcac cagactctct ttatgaattt
    15001 aagtgccttt atgctgtaga ttctttagca cgttctctag atacgctaag aaatagtctt
    15061 gagctcaaat gggacgcaaa gctttcggat acgttggctc ctgtcgttgc tgaaacagtt
    15121 cctgtaactg gatcaaaact agttttccca aatgaaaaaa tagaggtggc ttttaacaag
    15181 cctgtttctg gtgatacatt agcgagtttc ttaaaacttg ttatcaacgg tgatactgga
    15241 gatgtggttc ttaaacgaaa agatacagta cgctttgaaa tcagtactgc atcgccttgg
    15301 ccaacggatg gaaaagtgga gttgctcaat atcagccaag atacagttgt gaagcctgcg
    15361 gatagcacag ggtatcgaga tacagtcatt actcctaaat ttacgaccgt tgtaaaattt
    15421 gaaacggttc caaaattaaa aattgcttcc ttagagggaa aaattccagg cactaaaaga
    15481 actcttcccg tagttcgctt acgttcttta gaaacaggaa aagtgttgaa tattcgaaca
    15541 ggtcctcgag gggttttctc aatagataac cttcttgaag ggaattacat tttagattac
    15601 tattataatg aaagtactat ttcgaaccca gatgcgggct ctctaagccc agcaaaattc
    15661 ggcgctggct ggcgctctcc ttcagatact ttaattcttt tgaacggcaa aaataaactt
    15721 gattctttaa ttcaaaaatt gccgtcatta ccataagcag gattgatcat gacattaaaa
    15781 attccttcat ttgtagccat tgacttagaa actactggat tagattttga taaagatgaa
    15841 attattgaag tggctttagt tctttttgaa aatggaacac cagtaaaaac aagcgatttt
    15901 cttgtgaaac caaagcaaga tttgcgctct tttattgaag cgttaacagg gattacaaaa
    15961 gaagatttgc tttctgcttc tgattttgct gatatcgctg gccaagtacg tgcttttatt
    16021 ggggattatc ctattgtcgc tcataatgct cagtttgatt ataagttttt gaaaagtgct
    16081 ttttctaaag tcggtattaa ttttgagagc gaattagttt atgattcttt aactatttct
    16141 agaatcgcat ttcaagatgt gcccaatcat cgcttagaga cgctagtgaa tcatttgaaa
    16201 atagagcgca ctacagcaca tcgcgcgcta ccagatgcag aagcttgtgg taacttgttt
    16261 atcaaggcat tagagaccat agactcgttt ccagcatttg aaaagaaagc tctgtttcat
    16321 gtatctaaag gttccatttg ggaagctatt tttaatgaag atgcctctgg cacattcgat
    16381 tttgttcctg gtttagtaat agatgaaaaa gaatctgttt taacaaatac ttctgagaga
    16441 aaaccacgtg ttgctgaatt tcttgaaccc gatggatttt tgtctaaaac gctacccaat
    16501 tttgtttcta gagaaaatca gattgattac gccaatgtcg tagaaagaaa catgtttaaa
    16561 gggggcttga gcgtattaga agcagctaca ggaacaggga aaaccattgc ctatttattg
    16621 tctgctgctc taaaagcagt ctctggagaa cgagtggtga ttagtaccgc cactagagca
    16681 cttcaagaac aattatggca acacgaactc cctaaaattg agcctttgtt tgaaggcaaa
    16741 ttaaaagcga tggttttaaa agggcgcaaa aattatatct gctatcgtaa atttagagac
    16801 cacttgcaaa gttttgatac cttagtctct ccagaagaaa aagattcctt tatggcttta
    16861 atcccatgga tacaacgttc taaaacgggc gatatcaatg agaatagtgg ctttaatcat
    16921 ttacgtaatc gttctctctg gagtaaatta gcaagtgatg cgggttcttg tgctaacgaa
    16981 tcttgtccat tttataatcg ttgccctgcg ttagaagcca aaagaaaggc gatgcgttct
    17041 aatttattgt ttgttaatca tgccctattt ttatctgact tagcacttga ttttgcgatt
    17101 ttaccagcgt atgaacatat tgtttttgat gaagcgcatc gtttaccttc tttaagccat
    17161 caagcatttg cgcgttcggt tcgctttttt gatttgagaa atatttttaa atctttagtt
    17221 catctaaaag cggaagataa aggtttaatt gctgatattg aaaatagtct cttaaaatca
    17281 aaagaacaaa acgaagaggc tttagccctt tgttctaagc ttcgtacaga tatcatggag
    17341 tctgaacgtt tgctgcatcg cttctttatg aaaataggga aaaaagtgag caaacaaaaa
    17401 ggatcagaaa atacttttaa gtataagcaa agtcttttcg cagaacttga tgccgatcca
    17461 aaagccgttt tagaatctct agtagctgta aaacaaacga ttgcagggct ttcttcacaa
    17521 cttcgccaaa aagaatttgc gctgggcttg gttcgagatt tagaaggttg gggctctgat
    17581 attgaaaaag tccagtatga tttagatttt atttctagtg gtaataaaga caattgggtt
    17641 ttctatttag aagaaccatt taacccacat accctatcga tgaaggcagt tcctttagat
    17701 ataggcgtgt tctgggcaga tcatttttac aaatggatta aatcagcaac gtttacttct
    17761 gccactttag ctgtacaagg ttctctagat tactatgctg aacgcatggg aatgacaaat
    17821 tctttacctc agaaaaaaag gcctttcttc agactctacg acgcaggctc tgcgttccat
    17881 tcaaagcgaa aaattggaat tgcaaacttc ttaccaaaac caaatgctcc agaataccag
    17941 gccgaattag agcgctttct cgcagaaatt ttacctgaga ttgaaaaaaa tgtgttagtt
    18001 cttttcacaa gcatcacaag tatgctgaat gtgcacaaag ttttgatccc tctatttgct
    18061 caaaagaata aattgctttt gtgtcaacat gcagatggca ctatggatag tttagtagat
    18121 atgtttagaa aagagcgcaa agcatgtctt ttaggttgcc agaccatgtg ggaaggagtt
    18181 gatctccctg gagaagctct cgaagtatta attattccta agttaccatt ccctaatcct
    18241 tcggatccat tgatttcggg taaagcggaa ataataaaag aaaaaggtgg aaatccattt
    18301 aagactttat ttgttccaga aacctatatc aatttaagtc aaggaattgg tcgcttaatt
    18361 cgctctgaag aagatagtgg ttccatcatt ttgttggata atcgccttgt ttcagagcct
    18421 tatggcaaaa ccttcttgcg tatttggaat aatgaacaca aaatccttca taatcctgaa
    18481 gaatttaaaa acgccttgta agtcaaggct taccaaaacg ttttatttat tttaaatttg
    18541 ccccctatta aaggccaatt ttatgttttc tatttctgct ttttttaata atatgccaaa
    18601 atctctgttg agagatgtcc atgcgaaagc ctttggcaaa aaggggcttt taaataatac
    18661 tttaatagcc aaagagtcca tccaattttt tagtgataaa aaacgttttg aacatagcct
    18721 ttctctttta gaatcatggc aattcaattg cttatacttg atctatcgca gtgagtctcg
    18781 tggtttaacc tataatgaat tacgtttaac cattccagtc aataaatctc gggaattaaa
    18841 acaattttta ttaaatgctt ctcgtgagta tgctatttgg cagggaaaga ctactacaca
    18901 tgttctcttg tataaaggct ttagtgattt tgtagactgc tttgaaaata agggaataga
    18961 agtctatgca cctacagaga caacgggctc tttagagtac gattatttgt ttgattggca
    19021 tatctgtaaa attttaggca tggctgctaa gggtttatta aaagtcaacc taacagaagg
    19081 gcttcatcgc aggtcattta cgctttgtga aaaagcaatc acttattcga aagctctttc
    19141 aagcaaagcc cctcatgatg aaattctttt tatccttcat ttcttatcgc aaaatgaatg
    19201 gcttggtcga gaagagggac aattaaaacc gactccaaaa gcaaatcaat tcttaaagaa
    19261 aaatggattt cgtttacgcc aagatattgt ggcttggtgg attcaacaac gcttccgcgg
    19321 caatgaagaa cattttaagc gcttgcttga atacttgatc aatcctgtga atattgtcga
    19381 tgccgcttat ttattttggg taatcgatcc atcgtatcga attttagaac gcacaaaaac
    19441 attaacttgg gattatttac ctaagccact cagagaactt tggttcttag gttttataga
    19501 ttttaagtgc gctagagata aaattcatgc cgtctctcta acaaagattg gtcgagccta
    19561 tttaggaaac gaagttacgc cacttgtttc tggagatgtg tcttctttac caaattttga
    19621 attaattatc ggagcaaaaa attcaccgag aattcttttc tttatcgcga ctcttacgat
    19681 atctcaaaac gatgagcctc atttaaaatg tgtatttgaa aaggaatctt ttttagatgg
    19741 tttaaaatcg ggctttacag aagaagatgt tgaagagttt attcattgga taaagccacc
    19801 taaaaatgta ttagattcat tacatgattg gagttcgtgc ttttttggag caaagattgc
    19861 tacagttcgc ttattaaaaa tcgaaaataa aaagattctt gaagaactca tgcacttttc
    19921 tcattttatg gaattagtaa aagaatcaat tccagactat ggatttattt tagatcctaa
    19981 aaaagaagag ggtgctctta aacttctttc tcattatggc ttacttccaa atgtcaatga
    20041 aaaggtgatc tcaatagagc cgttcaaaaa tgcttcttgg gaaaaagaat ttgccttgca
    20101 ttggcctcct aattttgagc cagattatga gctaaaagaa tccttagaca aagaatcttt
    20161 tatttcgtct atagaagcca caaaatatgg tacgctttat cgcaaattag atttgatgga
    20221 tttaattaaa gtgatgcgct atgcaaagtc aacaaataca atgcttgccg ctcaagtgaa
    20281 aaatccagaa gaaaaacatg cagacatgaa agagttattc ttttatgctc aaacgttgca
    20341 tttttcaaaa acgccttatg tagcaaaggt gcaattaaaa gataccgaag acacgatagc
    20401 tttagatctc aatcacattc aggagattaa attggtctat caaggaattg aagcctagtt
    20461 aaggtatagc tagcaatatt aaacccgaaa ttatcacatc gtgctcgctc ctgaaattca
    20521 ggctagcgat gatctaagag aatttagaga aaatccttcg aatggaagaa tgtataaaga
    20581 ataaattata aaagagccaa tatttttgag aacagcaatt ttaacgttaa taaaataaat
    20641 aatgtaaaat attcttaaca atagtttgtt tttttatgaa aaactatgtt taatattgtt
    20701 tttgttcgat ttttacgaat ctgcttttac gtttggtctt tattttctta ttttacttaa
    20761 ttacctgctg ggattgtggt gggtgttata ataaaaaagg aataaataaa atgagaacat
    20821 ttagtactag aatcattcta ttcatgctaa ttgcttttaa tggattattt gctgcagtaa
    20881 gtccagattt tcctatcgca ggctgggctg tttctggaaa tacaacgggc ggagctggtt
    20941 atggagaagt aaccgtagat aatacttcag atttgaaatc ctatgctaaa gcaggcaata
    21001 aggttattta tgttaaacct ggaacttatg ctggtcaaat cgatgtgggt tcaaatgtca
    21061 ctttatatgg gtatccaggt gctattatta ctcaacctca tggtcagtcg gcaattaaag
    21121 tttctggtgt aagcaatgtc attattcgaa attttattgt aaaagggatt ggtgctgtag
    21181 atattgatgc taatgactgc gttcaaataa acagtggtgc ttctaagatt tggatagatc
    21241 atttggatgt ttatgatggt caagatggaa atttggatat tgtgaatgca tctgattacg
    21301 tcacgatttc atggacaaag tttagttata cttcagcgtc ttcagatcac cagtttagca
    21361 atctgattgg aaatagcgat agtaaaacaa cagatagcgg gcatttgagg gtcacacttc
    21421 atcataattg gtgggctgat ggggtgaaag agcgcatgcc tcgtgtccgt tttggaaggg
    21481 ttcatgtggc taataactta tttgaaacta aaacgagttc ttcttgtgtg cgagctggtt
    21541 ataaagccga tttaagagtg gaaaacaacg ttttcattgg agtaaaaaaa cctattgatt
    21601 tatatgaagg cgattatacc gctgtttctg tatcgggcaa ttattttgaa ggaactacag
    21661 ggaacacttc gggtaataaa accgctttta ctccttctta tagcatgcct ttaacagatg
    21721 ttagtaacaa ggctaaggct tatgccttga gagattctat taagaatttc gctggtgcta
    21781 ctttacctgc tccaggaacc gtttattctt cttcaagtgt aacacccagt tcttctagtt
    21841 cattacctcc ctcttcttcg agtgtatttt ctagttcatc atctctacct gaatcttcaa
    21901 gctcatccac tatccctgct tcttctagtt ccgtggatgt gagtgcccct gcggtcttag
    21961 aaaaacatgg ttcaggtagt tcaaatcaaa cagttgctgc tggtgaatct atagcctctt
    22021 tctattacac atggattaat gcttcgggag taacagtcga gggtttgcct gcaggtgttt
    22081 cttatgttct agatgaagaa aagaaaaacg tctattttga aggaaccgtc gaatctagta
    22141 ctcctgctgg tgattacact tttactgtga ctacagtagg taaccctatt actaatgcgg
    22201 taaagtcagg gaccatcaaa gtaacttcta caacctccat tgaatcctct tattttaaga
    22261 gttcaggttt tcaaatcact ccaaagatta ttcagagtga agctagtatt acctttaact
    22321 taaagaaaca aggcttagtt tcactttcaa ttattgatct ttctggtcgt gaggtgtttt
    22381 cgaaaaaatg gaatgcctcc acgggcatga atcaaatgat tgtgaatagt tcttctatta
    22441 ctgctggtgt ttattatgtg cagttaaaga cccctgcatc ggaatcaatt caaaaaatag
    22501 ttattcgata aaattctctc catatcgaaa gaagaaggat cgccgcaagg cgatccttct
    22561 ttttaggggg gaggagaaaa ttaaatggct ttaaccacaa acagatgccc aggtgcctta
    22621 ttaggatcca gacgaacaaa gttttgtgag ccacgccaaa caaaggattc gtcagtaata
    22681 agatccttga caaagaaaaa ggcgtcttca gacaaaccga gctcttgcat atttagagca
    22741 atagtccctt cctgagcatg atgcatatcc atattagcaa tacacaaaat gacatctttt
    22801 tcaagtttct ttgaatatac cattaaatta gtattttctg cgtaatgaaa tctaagatta
    22861 tcgtattctt gcatcgctgg atgggcttct cgcgctaaat tgactcgacg aatgaagtct
    22921 ttaatgcctg gttcgtgcca ttgatgcact ttatactggt atttttcact atcatgcagc
    22981 tcttctttaa ttggcgactt gatattttca caaagttcat agccattgta catgcctgtt
    23041 agactggata aagtagctgc taaaaaatag cgttgcttaa aagcattagg gccttgatca
    23101 gctaagtaag caggaaaaat gtctggagtc gtagggaaga aaataccacg catatattct
    23161 tttgcgtcag attgagttag ttccttaaga tactcttcaa attcccattt ttcagaacgc
    23221 caggtaaagt aggtgtaact catgtcaaaa ccgagtttac ctaagcgatg catgattttc
    23281 ggacgagtaa atgcttctgc taagaaaaca atctctggcc gtttttcttt aatgtcagaa
    23341 atgagccatt cccagaaggg gaatggcttg gtgtgaggat tatctatacg gaatatatcg
    23401 attcccttat cagcccaaaa aagaattata tctcgaatct cttcccaaag cgcttggaag
    23461 ttttcgttat agtagtcaaa ggggtagatg tcttcgtatt ttttcggtgg gttttctgca
    23521 aacttaatgg agccatcggg ttcatgaaag aaccattctg gatgttcttt tacataagga
    23581 tgatctggac tgcagtttaa ggcaatatct aaagcgagtc taaaaccctt ctttttgcat
    23641 tccttcacaa aatgttcaaa atcttccatt gtccctaatt cagggtcaac ggcataatga
    23701 ccgccaaagg aattccctac agcataagga caaccaggtt ctcctttttt tgccttcagt
    23761 gcattgtttg cgccttttct attggtcacg ccaatagggt gaatcgggac tagataaatg
    23821 gtatcaaagc ctaagtgatc aatgtaatca agcctgtttt cgcagtcttt ccaagtggca
    23881 ctcgcttttg gattattgcc ctggcttttt ggccacattt cataccatgt gcctgtacga
    23941 gcgtatttag gatccactct aagtttcatt acaggacttt ctgttggaat atcttcagga
    24001 gcaattgtcc aagcaattat tttatactca taaaacccga cattttgaac aatgaatttt
    24061 ccttcccata aatcattctc aacaaattcc atgggtactt gtttccattt tttttgtgag
    24121 gaatgtttgt ataaaatggc ggcatcgtat ttttcgtgac tatgtctaaa aatatctgcc
    24181 tgaatggtca ccttatcgcc tggttcacgc ttaatcataa agcgccctcc ctcaatggaa
    24241 gggcgaatat tttcaattac aaaattttct ttttttgttg gtattatagc catgctacaa
    24301 atataactag aactgctcta taaaagggta aaaaagatca ggtctttgat ttacagacta
    24361 atcaattcgg atcagttgaa tgttcttaaa ccaaaagatt cgttctgctc cacctaagtt
    24421 gaattctaag cgggcaaagg tgttgtcttc tacgcctaaa tattcaattt cataagattt
    24481 tccgttcaga ccaatgattg gcttctcttg gaatccaagg gtggtgtatg gcgtatggta
    24541 agatcctagg cgaacggtaa tagggccttc aatatttgac caagcggtga agatcagttt
    24601 gtacttacga ttttgtaaaa caggaatgtc ttcgtgaatt aattggacgc tgtaagactt
    24661 agagcctcct ttcgtcacat tcactttcat ctggaattca ttttttgatt cttgaataat
    24721 cgtggaatca gcaacaccag aatattgttc cattaatatc cagccagtag acccgttaga
    24781 aaagtcacca ttgacagccc agtttgtgga tatcttatca aattgagcta atgcttcaga
    24841 aacatagtct gaaatagtgt cgccgtcttt tgatgtccat gaccagcgta aacaagagaa
    24901 gctagataaa aagcgttcgt ttaaagtggt ccagagagcg gcattctctg tttcactaaa
    24961 gcgaataact ccgtttgtga ttctagcgga actaaaatca atgttgttaa tccaccgttg
    25021 gacaaagaag gtcactggta aattgagaga gtccttgtag atctctgctt gttgataatg
    25081 atagcgtaaa tcaatcgctt caaaagcgga aagagaatat tcaaatggga tatattcttc
    25141 accgattttt atggtgccag aaatagaata gggcttattt tgattgggtt tgaagccaac
    25201 accaatttct tttaaaattc cttcttcttg aaggtctatc ttcctaaagg cttcgtcaat
    25261 gacggactct ccagcatgta tttcatttcc gaaattgaca gcaagagtcg tgtcttcggg
    25321 catggtttcg tatggccata acaacagacc ttcagcagga tcaatggtga catagacgta
    25381 gtagctcgaa taagaacgaa tatgtgttaa gtgaaggcaa aaatcttcga tgatcaaggc
    25441 ttcattgtcg cttggcattt ttgcaagaag ggtattattt gactgttgag tgtcggactg
    25501 ggttccgtag tcaatgatga aactagcagt aaacgcaata cttggattgc caatttctat
    25561 accagcagaa ggttcttttt ctgcacaacc tattaaatag attgctagtc cgatcaagca
    25621 aaattggaaa aagcgataga cactcattac actcctcggg tcagaggaaa aagctggata
    25681 ttgacttgaa caatatcatc agcttctccg ttacttgcag agaaatctaa tagttctttt
    25741 cgcataagtt gcaaatgttt ttttaaagag gggaaatctt tacgcttaat cgcaaaagta
    25801 acggctgaaa agtccctttg gtcacccgaa agttccgata atgccgttga agcgagcttt
    25861 aacatttgat tatggtataa tttgaccaaa atatcttgga cttcatcatc agttgttaaa
    25921 agagggtctt tttgacgaaa tccgttcgct gttttgacta aaagtccaag atcgagcaaa
    25981 atttttaatg attccgctat ctgaacgggt gagactccac ccattacatg ctttgagatc
    26041 cattcgggag ccgcattaaa tccttttaat gaggtcattt ctaaaattac agaatgatac
    26101 cattcgcgga aaattcggaa ttgggctttt tctaatgagc gcagtttaga acgagggctt
    26161 gcttttaata attgagcgta ataaatctgc ttctctttgt cattttttgc ttgattaaaa
    26221 aagacaaggc tttcaaaata agcggctctt ttagcgtcga ggttcaaacc agaaattaat
    26281 ttcacaactg tatttttggt gatattacgt ttaccgtcga ttgctagttt taaatgggca
    26341 tgacttgaaa gccctgattt ttcagcaaag aatcgaagac tgaaagccga tgaagttttc
    26401 tttttatact catagtagtc gcgcaaaaaa acacgataat tcgtatattg caaaatatcg
    26461 ggttctttag gttttatgtc tgcgctttca atcatattaa taagatagct gttataaata
    26521 aataaagaca gtataattca tagaaactcg tttacgaaag taaacgaagc aaaatgtggt
    26581 tatagcttta aatggctcgg atcagttgaa agaagagaaa aacggagatg atttaagaaa
    26641 ataccatgga gattggcgta ttctctgaaa aagccttctt cttgaaaacc aactcgtttt
    26701 actaaagcaa tgctttttag attgtttgct gcaatatcta tttctaatct gtttaatttc
    26761 aatgagttaa aagcataaga acagatcatt tttagcgctt cagtggcgta accttttcct
    26821 gtatgggatt ttaaaagcca ataacctata gaagcagaga gattatccca ttgaatccat
    26881 tgtaaacata tacagcctaa aaaggttcga ttctcttttt caaaaagtcc ccatagacct
    26941 ccattatcca tttcgctttg taatgtatag gaatcaataa atcgaagagc gtcttcaata
    27001 gtgttcatgg ctctaaccca aggaagtttt gcatctaaaa aagaacgaga agaattcacc
    27061 tctcttaaaa aaatttcagc atgcttttct tcaataggtt ctagaacaat tctatttcct
    27121 tcaattatgg gcattattcg atgtccatta caaaaaaagc ttcttgacta tctggagtta
    27181 aaggagtcgt ggagggtatt ctgtttggat aatagtaacc agtgattctc gtaatttcgt
    27241 gattagaggt gtctttccaa ttgggaagaa ttccagaatt taaggaatct gataaataaa
    27301 taaaagtgtt taatcgtttt tctttagaat ctatttcgta taaaaagaag catggctttc
    27361 tttggcagag attttgatcg tattggtatg ttatatctcc agttagagta tagcatgatc
    27421 tcgctgtggc tccttctgcg ggaattttac gcatttcctc aatagaaatg ctttggcact
    27481 cctgaaagac atcttcagcg cagcccagaa gaaaagaaag aaaaagaacg aataatattt
    27541 ttctcataat aataacgata gtaaaaaaaa gactaacttg tgtagctagt ctttttagtg
    27601 cggatgagag gacttgaacc tccatgtcct tgcgaacact agaatctgaa tctagcgtgt
    27661 ctaccaattc caccacatcc gcatttttgt tcaaatcttg aaaaaaagaa aggctttgtc
    27721 aaggtaattt agggtgcttg aactgctgaa ttcgtttcgg gtgcttttga gtccaataac
    27781 tccagtcgtg ttctcctttg gagtcaaaaa aaacgttaca agaagaacca atggattcac
    27841 aaaaagaatt aagccaaggt aagacatgag aggctggata aagtcgatct tgaccgcctt
    27901 gcataaaaaa ccatgcccct gagcgaagtc tggtattttg agcaaatact ttggggtctc
    27961 cgacaataga taaattagaa ctaattaaca gtctagaaag aatggctcgt ttcccgttca
    28021 tagaataagc gaagactcca agggccccgt cagagactcc tactagatta acttctttaa
    28081 tgagaatatt aaaatgatgt tctaatgagt cgattaattt atctgtaatc ttgattaaag
    28141 taggagaaag ccagtgttta tctaaacagg cagatgggct agctataatc acatttggat
    28201 cttctaaaag ctcaaataaa ccttggccag ccacgagccc tttttgacat ttagagcttt
    28261 gcatccctcc atgaaaccaa ataatcacag aaggagaatt ttgttttttg ggtgttgaaa
    28321 accatacgcc tgcgggacta ttaaaatcct tttccattat gattagagag tcttttaaag
    28381 caaaaacttg agttaaga
//
LOCUS       DAOOBB010000005        25640 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_2533_length_25640_cov_4.848622, whole genome shotgun sequence.
ACCESSION   DAOOBB010000005 DAOOBB010000000
VERSION     DAOOBB010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 25640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25640
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2533_length_25640_cov_4.848622"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="PLT31_00570"
     CDS             complement(<1..1128)
                     /locus_tag="PLT31_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93656.1"
                     /translation="MLRYVDMLSFKFPLVALISLCFFVSAQGADGWDLLLQNKSKQAE
                     ESFLQIIKKNPKGKKAAEAYIGLSEVAKYNGNKSLANDYCLDAWDAYSDSQLFISCLY
                     KVSLQLYHSTQEKSQRTIRVTKQLMQKSPESLVATELWNAVSEVYIHEGKKDSAKLWN
                     EKLGFLNYWKYVGPFNNVSNGGLFTSFDVEKGFSFSEKYKGLSGRQVEWRSLYPEPIS
                     WIHLDYYQNIPDAVNYFATTVVSPKATKAFVNLGLSGVFSLWINGTKVMENSVFQNTG
                     VDIFRAPVTLRQGKNHILLKLGHEKKQSNFVLRFTDEKGFSILLPHAPEPQPAVENQS
                     KLKKNSVMNTTESSIKKAIQKNPKSILWKLASIQYRINTEQF"
     gene            complement(1249..3039)
                     /locus_tag="PLT31_00575"
     CDS             complement(1249..3039)
                     /locus_tag="PLT31_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPW93657.1"
                     /translation="MNRYKLLAFLGCFIFLLNGCDEKKTVKTSALENCPEKAVYDSNS
                     TGEYDPIASLDAVPCGAVNLWGSSMPKSFNMWQDYNSFSAGLMGLMFEPLVSLHSTED
                     REIGILAESFEISEDGKTFTFRLDPKARFSDGKKITAEDVQFYYDVIMDPKNLTPIFK
                     VGLSRFERPVIIDSLTLSITAKEVHWGNFWEAAGMLAFPKHLWKDLDFNSIRYDFPIV
                     SGPYKIKTFREDRFVELERRADWWGFQKKWNHGKYNFQTIRYRFMEDRNKALEAFKKQ
                     DFDAYAIYTSSIWMKQTDFEAVEKGWAVKQRVYNKEPIGFQGMVMNLRRAPFNDVRVR
                     RALSFLMNRELMNEKYMFNQYFLLNSYYPDLWPDNKNPESPFYSFNPDSARILLAEAG
                     YVQTEQGMLMKEGRPLSITFITQAEDLRHLTLYQEDLRKVGIQVQIDQMSQSTLRKRL
                     DDADFDMYWINWGASRLRDPEASWHSKTAMDKGTNNLAGLQDSVVDALIEMQKTEFDL
                     SKRNEILRRLDVRLNELVPYVLMWQSNHHRLLYWNRFGMPGTVLDKYNREESIPVYWW
                     LDAKKASTLKEAIASKKVLPKPEYDVFYKE"
     gene            complement(3036..3752)
                     /gene="rnc"
                     /locus_tag="PLT31_00580"
     CDS             complement(3036..3752)
                     /gene="rnc"
                     /locus_tag="PLT31_00580"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HPW93658.1"
                     /translation="MRLWFRHKSGDGLEAKLGYRFKDPALLAHALVHRSWIAGKDIPY
                     TENNERLEFLGDSILNMLVTEYLYKKFPDAPEGQMSKCKSAVVSGHALAQTSLLWDLG
                     TYIRIGKGEARMGGRNKENILADAFEAILGAVYLDSGLNSVREILERFHFPRIESIVA
                     GEEFVNHKSALLEVMQSKSKPLPEYKVIRESGPEHHKVFDVEVLLDGVVYGRGSGVSK
                     KKAEQEAAGAALEQLEKEKK"
     gene            complement(3862..4104)
                     /gene="acpP"
                     /locus_tag="PLT31_00585"
     CDS             complement(3862..4104)
                     /gene="acpP"
                     /locus_tag="PLT31_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPW93659.1"
                     /translation="MNEELIKKVTDVVVEKLGVKAEDVTLDSTFVNDLGADSLDRVEL
                     VMALEDEFDLEIMDSDSEKFIKVSDVVSFIESKKNA"
     gene            complement(4150..4890)
                     /gene="fabG"
                     /locus_tag="PLT31_00590"
     CDS             complement(4150..4890)
                     /gene="fabG"
                     /locus_tag="PLT31_00590"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HPW93660.1"
                     /translation="MINLKEKTAIVTGASRGIGLEITRVLASRGANIAMIATKENKEL
                     ASSLEAEFNVKIVSYACDVSSSEMVESTFKNIIETFGAVDILVNNAGITRDGLLMRMK
                     DEDFDDVLRINLRSVFICTKAVARHMMGRRQGRIINMASINGIRTQAGQANYAASKAG
                     VIGITKSNSMEFAARGVTVNAVAPGFIQTDMTAKLNEDTINKFKENIPMKDLGKPADV
                     ANLVAFLASDEARYITGQIIGVDGGLNA"
     gene            complement(4893..5813)
                     /gene="fabD"
                     /locus_tag="PLT31_00595"
     CDS             complement(4893..5813)
                     /gene="fabD"
                     /locus_tag="PLT31_00595"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HPW93661.1"
                     /translation="MSKNIFLFPGQGAQYIGMGSTLAQNFEPAKKMLEYADEILGFSI
                     SKIMKEGPEEVLKSTDNTQPALFVTSMMVMELLISEGITCDMVAGHSLGEYSAICAAG
                     GISFEDGLRLVRKRGELMAAAGTRSPGKMAAIVGAEDSVVEALCEEASIKGIVIPANF
                     NCPGQVVASGSVEGINALVELCELKAIKVVVLPVSGAFHSPLMQFAVEGLVEAISTVK
                     FSDLKYPLIANVTAEPVIDGSLLPDLLARQIISPVRWGASMQKAISLGVSSGVEVGAG
                     KVLMGLMRKIDRNIKIVPVENIADLAPLKG"
     gene            complement(5847..6818)
                     /gene="plsX"
                     /locus_tag="PLT31_00600"
     CDS             complement(5847..6818)
                     /gene="plsX"
                     /locus_tag="PLT31_00600"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HPW93662.1"
                     /translation="MIRIALDAFGGDYAPDVVIDGAVRAVKANEGKFQIVLCGPKAEV
                     EAGLQKLGYNGDAISVVDAPDIVAMDEHPTEVLRKKPNSGLVTCVALQKKGLVQASVS
                     AGNSGAMMASCLMLLGRSSENFTRPPIGCAIPTAKNRAVLVDAGANVDEKASVLVDFA
                     IAGSVFAECYLGYEKPKVALLNMGEEEKKGPAVIQEAYQLLKNTSLNFVGNVEGRDLI
                     AGEADVIVCSGYTGNVVLKMLEGFYEMHQEFFGTIDTPAGHRFAQMWDYRSTGGALLL
                     GLNGTGIIAHGRSDAKAIEQAVIAAYQYAESKVAENVGLKLAEYKHA"
     gene            complement(6840..7022)
                     /gene="rpmF"
                     /locus_tag="PLT31_00605"
     CDS             complement(6840..7022)
                     /gene="rpmF"
                     /locus_tag="PLT31_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPW93663.1"
                     /translation="MAVPKRKTSTARRDKRRTHWKMEVPAMATCDHCGAVKRPHRVCP
                     VCGYYKGVEVVRPEVA"
     gene            complement(7025..7546)
                     /locus_tag="PLT31_00610"
     CDS             complement(7025..7546)
                     /locus_tag="PLT31_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HPW93664.1"
                     /translation="MKIEILQTMVEPVTKEYLWNRSACPALFDELHLVDNLSAQVLIS
                     PQSKDKWLVTGSLSGVQLLTCVRSLDLFNRPFETDLAIEVERTQRVAKQEMSDEDEEV
                     FRFLIPIVQSEVDISECVRQLVLLQEPLNPMKNPDEEFICPSTESSDEPTEDLRWEKL
                     KALKRKMENSNRS"
     gene            7705..8751
                     /locus_tag="PLT31_00615"
     CDS             7705..8751
                     /locus_tag="PLT31_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93665.1"
                     /translation="MTLSILFYFFIFLLLLGSFYYSIEQLFSYLSIKKVQSHYSITEP
                     LPKVSVVVFFDSTYFHSETIEQLLEQNYANFYDIWVVGKESKDFETNPKIQLFPIDHE
                     YSFNELEILKTIASMSEGEILCSIHNGNTISSEWLSSMVREFEPGVEIVKAPIRYTYS
                     PNETIIQKSIATYKLFLDLSNTSKIIRQKSLFLSSENTGFLKSFLISNFQTEFLAAAF
                     NKPGRIQTTNNPNTILEKSNQEKDFFSIKQFLNTALINTSFLTKTYFIILTFSVFFSF
                     FSFYLLLAIFFTWILKLGMDFLFIIRGMKISGTPISWKSFLLFELLQAPLITISSLLS
                     LYRFLRMKKQGSLF"
     gene            8751..11234
                     /locus_tag="PLT31_00620"
     CDS             8751..11234
                     /locus_tag="PLT31_00620"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HPW93666.1"
                     /translation="MNKKTEKSKLTLIIAEKPSVAADLVKALGAKNFKKEKTHFESDT
                     TIVSWAIGHLVTIADPKNMDERYKSWDMSTLPIIPDQFVIMPIPETKSQLSALGKFIR
                     NKDVSTIINACDAGREGELIFHYIIEHEKGKTGLKNKEIKRLWMQSMTTSAIKEAFEN
                     LRSSEEMQKLQDAAISRSEADWLIGINGSRGLTAYKSSFGGFQLTPCGRVQTPTLAMI
                     VKREDERNEFVPQDYWTILGLFKNNNQEYEGKWFRTEGKENVQRIWEQGTAEAIVKKC
                     EKKTGTVTETTKPSSQKSPPLYDLTSLQREANNRFGFSAKTTLQIAQALYERHKLTTY
                     PRTDSKCLPEDYISTVKKVLESITGPLAVHAQNVLKNNWVKKDPKVFNNKNISDHHAI
                     IPTGNVPKSLSEAEQKIFTLICQRFISVFFPPAKYLNTTRITVIEEETFITEGKVLED
                     PGFKAVYGKDADTEQTLPPLGKSNEAKNLEIKVNQEVTKPAARYTESSLLSLMESAGK
                     LVEDDDLRDALKDRGLGTPATRAAIIEKLITDKYVVRDAKELVPTSKAFDLIRLLSAM
                     NIEPLTSAELTGEWEHQLGLIEKGEESRESFMKGIIGLTQQIVSSIKSFKEEDTKKEA
                     SFSPVNGIRVYDSVSRYETEDGIMIRKMLGGRLINEEEVQTLLLKRQIGPLSGFRSKR
                     GASFSAVITINDKNKVEFLFDNADNDVEVGEQIGISPIDQSPVFDSLTAYISESALQK
                     EKTGFRLSKMILGKEISVENIKKMLAGEKSDLIQGFRSAKTKRLFDAYLTLDKNGKIK
                     FNFPPRVASAKKTFARKKTTKASESSDDN"
     gene            11265..>12281
                     /locus_tag="PLT31_00625"
     CDS             11265..>12281
                     /locus_tag="PLT31_00625"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPW93667.1"
                     /translation="MPYSVKTERTFFQKFLFGILLLQFSLAFANTTTTKMAIPEEIRP
                     LWDSLSLKEKIAQMVMVYMPNEAYITTHQFGAILVMKPHLKDTNQLKETIFNANQNIK
                     VPLLVAIDQEGGFVNRLSNADPKWSGLPSAIAMRSMPTQAIDSIGEITGKALSSLGIN
                     LNLAPVLDPATDSRGKKTFIEESSRSWGNLDSTNINHVRAFVKGLARSNVFCVSKHFP
                     GYDSWTNSDHQISMSSAPDSQIQKNILPFKMLVEDVPFIMMSSVRFRKISESPAVFEK
                     KLVKQAKALHPNTIILTDDLWGMSLRSWISGRKNLKRKNYPKADFEKLIHQFSYILTF
                     YYFTF"
     assembly_gap    12282..12291
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12410..12973)
                     /locus_tag="PLT31_00630"
     CDS             complement(12410..12973)
                     /locus_tag="PLT31_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPW93668.1"
                     /translation="MRFAIVDLETTGGQPKNDRIIEIGIVLMDGTEIVQTYESLVYPG
                     RFIPPFVQSLTGIHSEMVESAPPFMDVAETVYELLEGRIFVAHNAPFDFGFLKVELAR
                     ACCKWDPPRLCTVKLARKVFPGFPSYSLHSLTQSLGCSDFKHHRALGDAIAAAEILKL
                     AINKVGENGITKEIKNLSASKVRTYIS"
     gene            complement(12973..13635)
                     /locus_tag="PLT31_00635"
     CDS             complement(12973..13635)
                     /locus_tag="PLT31_00635"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPW93669.1"
                     /translation="MNDAVIIKTASDYDAVIIWMHGLGADGHDFEDVIPSLSLPENLK
                     IKFIFPNAPIVPVTINNGYKMRAWYDITSMDIARDPDIKGIHSSAERIASWVEREIGE
                     EILPSRILLAGFSQGGVIALELGLRFPKRLGGIIALSTYSPTLSLLHSEKSEANQKTP
                     IFWGHGLVDEVVPFALGKGTSDALIEQGYSVDFHEYASLPHSVSLSEIQEIGAFITKV
                     LS"
     gene            complement(13671..14234)
                     /locus_tag="PLT31_00640"
     CDS             complement(13671..14234)
                     /locus_tag="PLT31_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93670.1"
                     /translation="MNRLIFSLFFLIGLGFASQMATTYNGATVILHDDGRWEYYQNNE
                     KVRDLRPSSIPEEAKVNIYVMHESYEKLRKNKRLELEADFAPEEEILDSLRQIPKGGI
                     IHFCVPTEQLKPHAVKTYTYSVWNGGNKPVFQTTKPDSEVEPSDESGVSYLLSVPIYS
                     RIKVKTIKARIENKAGKQTVDFDVPVN"
     gene            14481..15365
                     /locus_tag="PLT31_00645"
     CDS             14481..15365
                     /locus_tag="PLT31_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HPW93671.1"
                     /translation="MTHNIRFAPSPTGYLHEGHLLSALYVWAMAKLKNYHIHLRIEDH
                     DLSRARNEYIDSIYEDLEWFGFEWDSESKQSDRHEIYETALKKLNKENLIYGCHCSRK
                     SLSMENPSSPDGEVIYQKHCLNKALPITSPNTIRLKVESTLIQWNDLRLGFFEENPAL
                     QCGDFALKDRLGQWTYQFAVVIDDMEENIGMIVRGEDLRNSTARQILLMSLLGKTTPP
                     LYFHHSLLYDPQTGLKLSKRQQSQSLRAERMNAIAPEEILGRLCFKQHLIKNKCSISL
                     NDAILLFSKQIEGYLQQK"
     gene            15379..19488
                     /locus_tag="PLT31_00650"
     CDS             15379..19488
                     /locus_tag="PLT31_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93672.1"
                     /translation="MSKSISSSQISKLQLEVLDSCIQNRLLNFPAQSVFQSPLIKENS
                     DVFIDKWIKSGKSFSLSSFFGNESFSFFEQILRRKQEDFGVFDLYLATGFLKWTGNKM
                     APVLLIPLKVNLENLTVEISDLVPIENIPLREQAKNEVALPKINDVLIDGRPDLEKYF
                     GMVEMAISKQPLWKFSRRGLCLSFFSTSHLYLYKNFDFMLNSESFKYSENSLFSKLFS
                     DEGFRIATSIFDEKEANDAYQPTDRAFLYPTDFSTVKATVDALNPKNEAYVIQTPPGT
                     FAFETAANIIADSLYNHQKVLVLTKRSVTTDKIKKTLFPEFRSTKGTDRSEIVNELNQ
                     IRNDLASYYKIVNSETTTGKISINDLFEEKINNPTIYNKIPDSVFNGIERLQYQEYKA
                     VKKTIETLVHEFSDPTTQKALETFKSCNIKVLDKDQQNKLAQLLSPTIESIDYLTPFI
                     DVLEAQTIFDTPLTQDTFAQIFSIIHKYFDKDTPSYEGWDLGSSDWDNYTENLLQIPE
                     SGSIWARYRRDGSLIYTEAAIDESVLAIRDEFAEITKNSFKAFSDNYRSRKKKLLSLF
                     KEPKEIDSDDELILLIDELIELQNHRNFYKNSAPMANRLFGKDWKYEKSNWVLLHVKQ
                     KYFYELKNKIKDHETASLTFKILEKLHLLKNLDFDIEAIEAQLNVIQKNNIEISKLLS
                     LINPLESIPLIEQANLLRNWMDGLSDLDIQVKTNAWIDRLKQKNLHSLIDYIFDKTAP
                     KEELIPSLVQYWCQNQISELTKEKKDLFTVSPQKRSQTSKKYRSLIDEYCNANFRFIE
                     EMIKKFPESLQISNICDYYAVSQKEPQKYDLVLILDAEIISSTECISAILSSEKMIVM
                     GDIENPELELLFTDGFENTDTLQKISVSKQKSILSQVLQKGAGFRTLSFSRLYQHPAL
                     FYFINENFYKNTIQQFPLPHKSNFKGLGLKKVADPIQTIVEQAVQHFKTKPTQTLGII
                     AFSENQCQEIRKELEDIIEQNAFLKKALYGTDLSRYCFIKTPERAVGLYRDTILVCAE
                     QDLNYNTSITKGKIKVSASLAKQEVLVFISSIQELSNSPKNDLLSLWFEYIHKHKTID
                     YYQIKEAQSPLLRFVQSTFDKNQISYEVGWSLNNTSICLCIYDNNNPNRFLAAIEDDS
                     YPKYLKESVEDIEYMRPMCLGNLGWKMLYMWSPIWISSLQDEIDHLLATISIEQSVAP
                     IQQETSASTEPQADFVIEESTKPEISVDPYIIKNPQIEGTEHHKPIPELSLKSIISQL
                     LFYIDKESPIHNDCLRRRVLQLHQLDREGPKITQILDNALKQGIQAKAFVKTGPFYYS
                     LKQKEIKLRYRGDLNPNERKLSYVSPEERALIPSSMDNASVRQLLGLLE"
     gene            complement(19492..19896)
                     /locus_tag="PLT31_00655"
     CDS             complement(19492..19896)
                     /locus_tag="PLT31_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPW93673.1"
                     /translation="MHKVFFLVFLFSSFLFADPTDLDSLFRGKEYRPVLNESLRTTTE
                     KIVEMAPSTSKSDKNSDGYYMLQFEAVADFDAAQRRKAQLSASTGYAIQIVFDTPFYK
                     LRGGGWSSKAKAEDKARELSVSNINALVIKVR"
     gene            complement(19980..24410)
                     /locus_tag="PLT31_00660"
     CDS             complement(19980..24410)
                     /locus_tag="PLT31_00660"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibro-slime domain-containing protein"
                     /protein_id="HPW93674.1"
                     /translation="MKQNNTSIKTVVFGLVLLLMGMGGAYAMSCSGTVHFQKPTTWAS
                     VYIYAGGAYGNVTPQCNATTGWCDIALGPNVPTSSQTYEKNFVFNNTGVSHPANWISR
                     TVYDFAGQAPEASAAFTCSDFGAGTDLYIYDNPSEAGKTVISTDPPNAKYLYVLIPPE
                     KEEWMSAVPMISMDGGATGVPMSADEKRCGWYYHVWFSDAPTEQVIIYRDDDSTKADA
                     IGLNGLWDEDGVVDPIPMATYFALYGVNDLYFIPDDNLWPDEGASLGWYDVDPMVDGS
                     CSYNLAAIIYDTDASLHPSFSCWSEGGEGCQVGAQGIPQATAVAAVNSCIGVTPGIVE
                     DLLDPVTNKPVLKAGTGTSCFHSAAIFNQLFNETPGVNEMSCFDMPFSRSDDGRWEFD
                     SDYFTSPGLTVQGGFYPVETTTSANIFSYSGTPTPAPKARTKRTAEGPVFIGPLLREL
                     DPVEGVQKMDLMCNGAGWSGGYDCSTLFNDGKDLETIMGQMFPTEANPCVWGWSCENM
                     APNGWPKYAKETENLASASTTDATGRWTSKATGVSGGRNQHFCFESHANFKHKPGLRF
                     SFRGDDDIWVFIGGKLAVDLGGTHLAAPGYVNLDQIKDKDDLPLVVGEEYPIDIFFCD
                     RRTTMSNVRIKTNMYIQQKTGISYNVTKPAEGKGYELCWQESGDGSCAAAMGGSSGTT
                     ECCGAAIESTCGQKIDISLTRRDGSVVPRGDVADLAAAGVYFGGIDLTNRHTPVINVE
                     NMQGLSPGTYKLVMGIGNKSTYVTFRVSGSVDVVVRDAIDPAVSPSPTWKFVSSAMAG
                     TKIPIYVSSVADPGTGNLELDLEGAAGQTYSLTTSKGLNLYADSSSDVILAASDFPRT
                     IESSGIDTLWATVPLAAMTASTEAHTVQVAGRTSVAALTFYAPQIVFADTIFADSSVN
                     KITTLADTMWVGSAYNVRLVAINPITEKVCSDCNFAVNSGSQTSNKVETLPATIDIVN
                     GEAVVTFRSLTEYRDVAATLSLVGPNAALLSATLSPLYFKDPPVPFPVLAEIFDKHGV
                     PSARTLPIPTPYFSTTQEYLDGIADSLVLHYHRAFHKDSLPDSVFVKWDLKAISGDIL
                     DSIKIDKAKIVAGTNCDQATSVCDSIIRIGGLEFSKEIQTVGSPQTIISSWSTYLDKT
                     VPVALASQTLITDRIAPILLKGRVSNVKTSPDFDRVALLASEPVLIKDQTAATSYGSS
                     AFTYYLKSATEVGNDPANKYIHVTASSGDVLAARDTVVMLYNNKDNLRPSPRAGDYVR
                     FRADMSDLISDVQGNFPTNYDADVASPWAPIEGDAKSEVLTISLGTPDPDKIKKDMEE
                     GKVVEAIPVDIYDSKDTIQAKNPNTLGHIVKTDMGNILTAPEFIDVKAEEVTLSIKTQ
                     YFTNLGSFVAESNHNIRCDAPFFNGNYPADTSSKGDCRQNPKYVYVAWNMLSQDNRKV
                     GTGAYIAKLSTYVNIQGRKKTAKHDQTEMWGVRRGGKVLK"
     gene            24734..25621
                     /locus_tag="PLT31_00665"
     CDS             24734..25621
                     /locus_tag="PLT31_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPW93675.1"
                     /translation="MQDLEKTIVTKSQTIRLPELETRAHLIILYPRELFRQIPLKTGK
                     NILGRGIEADIRLDDGLVSRRHCEISWDGKTVRIRNLNSTNGIFIDGQEVEEAVLDSE
                     NRIQIGKMVLKIDFKDASEEAFDRELFEAATTDPLTKISNRRTFLDRCSGECALARRN
                     DYYVHTLLIDVDHFKRVNDQWGHQAGDMVLKEIARLLKDEKREGDLLARYGGEEFILL
                     FSGIGLEDALRSAERLRLAIEKQIFVWNDITIPVTISLGLASRKGKAIPKIEEMISEA
                     DKFLYIAKDNGRNQVVVAS"
BASE COUNT         8545 a         5233 c         4163 g         7689 t
ORIGIN      
        1 aaattgttct gtattaatcc ggtattgaat acttgctagt ttccataaaa tcgatttagg
       61 attcttttgg attgctttct taatactcga ctctgtcgta ttcatgacac tatttttctt
      121 caacttgctt tgattttcta ccgctggctg aggctctggt gcatgaggta aaagaattga
      181 aaatcccttc tcatcagtaa aacgcaaaac aaaattggac tgtttttttt catggcccaa
      241 ttttaagaga atatgatttt taccctgccg taaagtcaca ggagccctaa aaatatctac
      301 gcctgtattt tgaaaaacag agttttccat cacttttgtg ccgttaatcc agagagaaaa
      361 aactccactt agcccgagat ttacaaaggc ttttgttgcc tttggactca ccactgtcgt
      421 tgcaaaataa ttaaccgcat ctggaatatt ttgataataa tccagatgaa tccaactaat
      481 tggttcagga tataaagagc gccattccac ttgtcttcca gacaaacctt tatacttctc
      541 agaaaaagaa aatcctttct ctacatcaaa acttgtaaat aacccgccat tactcacatt
      601 attaaaaggc cccacatact tccaataatt aagaaagcct aacttttcat tccaaagttt
      661 agcactatct tttttacctt cgtgaatata aacttcactt accgcattcc ataattcagt
      721 cgccacaaga ctttctggac ttttttgcat caactgtttc gtcacacgaa ttgttctttg
      781 gcttttttct tgagtagaat ggtatagctg caaactcact ttgtaaaggc aagaaataaa
      841 tagctgagaa tcagagtaag cgtcccaagc atctaggcaa taatcatttg ctaatgattt
      901 attaccatta tattttgcga cttctgaaag tcctatatat gcttcagcgg cttttttccc
      961 tttaggattt tttttaatta tttgtaaaaa agattcctct gcctgcttgg atttattttg
     1021 aagcaataaa tcccacccat ctgcaccttg agcagaaaca aagaaacaaa gactaatcaa
     1081 tgctactaaa ggaaatttaa aggaaagcat gtcaacatag cgtaacataa aatggttctc
     1141 ctggcataaa aacccaatca ttaagtctta aaaactaagg ggatatttcc cctccaataa
     1201 tgaattcatt ttgttaagac ctgaattcca tgatttttgc ttttttactc actctttata
     1261 aaatacatca tattcaggtt ttggtaatac cttttttgaa gctatagctt ctttcagtgt
     1321 agaggctttt ttagcatcta accaccaata tactggaatc gattcttctc gattatactt
     1381 atctaaaacc gttcctggca tcccaaagcg attccaataa agtaatcgat ggtggttact
     1441 ttgccacatt aatacataag gtactaattc atttaagcga acatctaaac gcctgagaat
     1501 ttcattcctc ttagataaat caaattccgt cttttgcatt tcaattaaag catccacaac
     1561 ggaatcttgt aaacccgcta aattatttgt tcctttatcc attgccgttt tagaatgcca
     1621 agaggcctca gggtctctca aacgagaagc cccccaatta atccagtaca tatcaaaatc
     1681 agcatcatct aaacgttttc gaagagtact ttgcgacatc tgatctattt gcacctgaat
     1741 tccaacctta cgtaaatctt cttgatagag agtcaaatgc cttaaatctt ctgcttgtgt
     1801 aataaacgta atggagagag gtctgccctc tttcattaac attccttgtt ctgtttgaac
     1861 gtatcctgcc tcagccaaaa gtattctcgc actatcagga ttgaaactat agaaaggaga
     1921 ttctggattt ttattatctg gccataaatc aggataataa ctatttaaga gaaaatattg
     1981 attaaacata tatttctcat tcatcaattc tcgattcatc aaaaatgaca aagcacgacg
     2041 aacccgtaca tcattgaaag gcgctcggcg caaattcatt accattccct gaaaaccaat
     2101 aggctcttta ttataaacgc gttgctttac agcccaaccc ttttctacag cctcaaaatc
     2161 cgtttgtttc atccagatgg atgaagtata aattgcatag gcgtcaaaat cttgcttttt
     2221 aaatgcctcc aatgctttgt ttcgatcttc cataaatcga tagcgaatgg tttggaaatt
     2281 atactttccg tgattccatt ttttttgaaa tccccaccaa tcagcacgac gctctaattc
     2341 cacaaaacga tcttctctaa atgttttgat tttatatggc ccactcacga taggaaaatc
     2401 ataacgaata gaattaaaat ctaagtcctt ccaaagatgc tttggaaatg caagcattcc
     2461 tgcggcttcc cagaaattac cccaatggac ttcttttgct gttatagata aagttaaaga
     2521 atcgattatt acaggccgtt caaagcgact taaccccacc ttaaatatag gagtcaaatt
     2581 ttttggatcc attatcacat cataatagaa ctgaacatct tcagcagtaa tctttttccc
     2641 atcactaaaa cgtgcttttg gatctaagcg aaaggtaaag gttttcccat cttcagaaat
     2701 ttcaaaagac tctgcaagaa ttcctatttc acgatcttct gtagaatgta gacttacaag
     2761 aggttcaaac attaaaccca ttaaacctgc agaaaagcta ttgtaatcct gccacatatt
     2821 aaaagattta ggcatggaag agccccaaag attgacggct ccacaaggca cagcatctaa
     2881 agaagcgatg ggatcatatt ctcctgtaga gttagaatcg taaactgctt tttcaggaca
     2941 gttttctaaa gcacttgttt ttacagtttt cttttcatca cagccattca gcaaaaatat
     3001 aaaacaacct aaaaaagcta atagtttgta tcgattcatt ttttctcctt ctctagctgc
     3061 tcaagagcgg caccagcggc ttcttgttcg gctttctttt tagaaacacc acttcctcgt
     3121 ccataaacaa caccatcgag aagtacttcc acatcaaaaa ctttatgatg ttcaggcccc
     3181 gattctcgta tcaccttgta ttcaggaaga ggctttgatt tagattgcat cacttcaaga
     3241 agagcactct tatgattgac aaattcctct cctgccacaa tggattcaat acgaggaaaa
     3301 tgaaaccgtt cgagaatttc tctcacagaa ttaagcccgc tatccaagta taccgcacca
     3361 agaattgctt caaaagcatc agccaaaata ttttctttat ttcgaccgcc cattcgggct
     3421 tcgccttttc ctattcttat ataagtacct aaatcccata ataacgaagt ctgagctaaa
     3481 gcatggccag aaacgactgc acttttacac ttagacatct gaccttctgg ggcatctgga
     3541 aattttttat acaaatattc tgtgacgagc atattcaaaa tagaatcccc tagaaattcc
     3601 aatcgctcgt tattttctgt ataaggaata tccttaccag caatccaaga ccgatgcact
     3661 aatgcgtgag caagcaatgc tgggtctttg aatcgatacc caagcttcgc ctcaagcccg
     3721 tctccagact tgtggcgaaa ccagagtctc ataatttttc gaataacgtt tgtttttttc
     3781 ttcaaagaat taccaccata aaaaaaggca acctaaggtc gccttaattg attttcaaac
     3841 aaaaacaatc cgaactttta attaagcgtt cttcttgctt tcaataaagg aaaccacatc
     3901 ggaaaccttg ataaactttt cagaatcaga atccataatt tctagatcga attcgtcttc
     3961 gagagccatg actaactcaa cgcgatccaa tgaatcagca ccaaggtcat tcacaaaagt
     4021 agaatcaaga gtaacgtctt cagctttaac accaagcttt tcaacaacta cgtctgtgac
     4081 ttttttaata agttcttcgt tcattttata actccattat aaggtttgag ataaatttag
     4141 taataattat taagcgttca acccaccatc tacaccaata atctgacccg tgatataacg
     4201 agcttcatca gatgccagaa aagcgactaa attcgccaca tctgcgggtt ttccgagatc
     4261 tttcatcgga atattttctt taaatttatt tattgtatct tcgttcaatt tcgctgtcat
     4321 atcagtttga ataaaaccag gagcaacagc attcactgta acaccgcgag cagcaaactc
     4381 cattgaattt gattttgtaa ttccaatcac gcctgcttta gatgccgcat aatttgcttg
     4441 acctgcttgc gtacggattc cgttgatgct agccatattg ataatacgac cttgacgacg
     4501 tcccatcata tgacgagcca ctgcttttgt acaaataaac acagaacgca aatttatacg
     4561 caatacatca tcaaaatctt catctttcat acgcatcaaa agaccgtctc ttgtaatgcc
     4621 tgcattattg actaaaatat caacagcacc aaaagtttcg atgatatttt taaatgtaga
     4681 ttccaccatt tctgaagagg aaacatcgca tgcatacgaa acaatcttaa cattaaattc
     4741 ggcctctaat gaagaagcca attctttgtt ttctttcgtt gcaatcattg caatatttgc
     4801 tccgcgagaa gctaaaactc gagttatttc tagccctatc ccacgtgaag cgccagtgac
     4861 tattgctgtt ttctctttta gattaatcat aattatcctt ttagtggtgc aagatctgca
     4921 atgttttcaa caggaacaat tttaatattt ctatctattt ttctcattaa tcccataagt
     4981 actttccctg ctcccacttc tacaccagaa gagacgccta gagaaattgc tttttgcatt
     5041 gaagcacccc aacgaacagg gcttataatt tgacgagcta aaagatcagg taataaacta
     5101 ccgtcaatca ctggttctgc agtcacattt gcaattaaag gatatttcaa atcagaaaat
     5161 ttcactgttg aaatggcttc tacaagacct tctacagcaa actgcattaa aggactgtgg
     5221 aaagcaccag acacaggaag aacaacaact ttaattgcct ttaattcaca taattcaaca
     5281 agagcattaa ttccttctac gctaccagag gccaccactt gaccagggca attaaaatta
     5341 gcaggaataa caataccttt tatagaagct tcttcacaaa gagcttccac aacggaatct
     5401 tctgcaccga cgatggcagc cattttgcct ggagaacgtg tgccagcggc agccattaat
     5461 tcgccacgtt tacgtactaa acgtaaacca tcttcaaagc ttatgcctcc agcagcacaa
     5521 atagctgaat attcacctaa agaatggcct gcaaccatgt cacaagtaat cccttcagag
     5581 attaaaagtt ccatcaccat catggaagtc acaaaaagag ctggctgtgt attatccgtg
     5641 ctttttagaa cctcttcagg cccttccttc ataatttttg aaatagaaaa gcctaaaatt
     5701 tcgtccgcat attcgagcat ttttttggca ggctcaaaat tttgtgcaag agtagaaccc
     5761 ataccaatat actgggcacc ctgtcctgga aataaaaaaa tgttttttga cataaaaatc
     5821 ctacaagaat gaatgtgaaa aaattatcaa gcgtgcttgt attctgcaag tttcaagcca
     5881 acattttctg cgactttact ttctgcatac tgataagcag cgatcacagc ttgttcaatc
     5941 gctttcgcat ctgaacgacc gtgagcaata atacctgttc cgttcaaacc taaaagtaag
     6001 gctcccccag tagaacgata atcccacatt tgagcaaaac gatggccagc aggagtatca
     6061 atagttccaa agaactcttg atgcatttcg taaaagcctt ctaacatttt tagaacgacg
     6121 tttcctgtat aaccagaaca aacaatcaca tccgcttcgc cagcaattaa atcacgcccc
     6181 tcgacgttac cgacaaaatt taaggaagtg ttttttaata attgataagc ttcctgaata
     6241 actgcgggac ctttcttttc ttcttctccc atattcagta aagccacttt tggtttttca
     6301 taacctaagt aacattctgc aaatactgat ccagcaattg caaaatcaac taatacagat
     6361 gctttttcgt ccacattggc acctgcatct actaatacag ctcgattctt tgctgtagga
     6421 atagcacaac caattggtgg acgagtaaag ttttcacttg agcgaccaag taacataaga
     6481 caagaagcca tcatagctcc tgaattacca gcactcacac tcgcttgtac taaacctttt
     6541 ttctgtagtg caacacatgt gactaaaccc gaattgggct tcttacgaag cacctcagtc
     6601 gggtgttcat ccattgccac aatatctgga gcatccacca cagatattgc atcaccgttg
     6661 tatcctagtt tttgtaatcc tgcttcaact tctgcttttg gaccacaaag aacgatttga
     6721 aatttacctt cattggcttt aacagcgcga acagctccat caatcaccac gtcaggagca
     6781 taatctccac caaaggcatc aagggcaatg cgtatcatag gtttctccta catcatattt
     6841 tatgcaactt cagggcgaac gacttcgacc cctttgtaat acccacaaac tgggcaaaca
     6901 cggtgtgggc gctttacagc accgcaatga tcacaagtag ccattgcagg gacttccatt
     6961 ttccaatggg tgcgacgttt gtcgcggcgg gcggtcgagg tttttctctt tggaacagcc
     7021 atcattaact cctgttcgaa ttttccattt tgcgcttaag cgctttcagt ttttcccaac
     7081 gaaggtcttc tgtcggttca tcggatgatt ccgtgcttgg acagatgaat tcttcatctg
     7141 gatttttcat cgggttaagt ggttcttgta gcaatactaa ttgccgaaca cactcagaaa
     7201 tatctacttc actttgaacg attggaatca aaaatctaaa gacttcttca tcttcatcgc
     7261 tcatctcttg ttttgccaca cgctgagttc tttcaacctc tatcgccaga tcggtttcaa
     7321 aaggtctgtt aaataagtca agcgaacgaa cgcaagtcaa aagttgaacc ccagaaagag
     7381 aacccgttac aagccattta tccttacttt gaggactaat taacacctga gcggaaagat
     7441 tatccactag atgaagttca tcaaacaaag ctgggcaggc agaacgattc cacaaatatt
     7501 cttttgtgac tggttctacc atggtttgta atatttctat tttcaaggta acggcaaata
     7561 tacaaggtct gaggggagtt gtcaatattg atgcatcctt aaaaaggacg ctaattatta
     7621 gaaatccgct cttttttaag attttgtctt taaaatgata aaaaaaaaat tattcattaa
     7681 ttcttgaatt aaattttgct caaaatgact ctatctatat tattctactt ctttattttt
     7741 ttgctcctct taggttcgtt ctattattct atagaacagc tgttctccta tttgagcatc
     7801 aaaaaagttc aaagtcatta ttctataaca gagcctcttc caaaagtcag tgttgtcgtt
     7861 ttctttgatt cgacttattt ccattctgag actatagaac agctcttgga acaaaactat
     7921 gcgaactttt atgatatatg ggttgttggc aaagaatcca aagattttga aacaaatcct
     7981 aaaatacaac tttttccaat tgatcatgag tattctttta atgagttaga gattcttaaa
     8041 actattgcct ctatgtccga aggagaaatc ctctgttcta ttcataatgg aaatactatc
     8101 tcttctgaat ggctaagttc tatggtgaga gagtttgaac caggagttga aatagtcaaa
     8161 gcccccattc gttacactta ttctcctaat gaaacgataa tccaaaaaag catcgccacc
     8221 tacaaattat ttttagacct ttcaaataca tcaaagatca ttcgacaaaa aagtcttttt
     8281 ctctcttcag aaaacacagg ttttttaaaa agctttttaa tatcaaactt tcaaactgaa
     8341 tttttagcgg cagcctttaa taaaccaggt cgaatacaaa ccacaaataa tccaaatacc
     8401 attctagaga aatccaatca agaaaaagac tttttctcta ttaaacagtt tttaaatact
     8461 gcactaataa acacttcttt tttaacgaaa acctatttta tcatacttac attctccgtt
     8521 tttttctctt tttttagttt ttacctttta ttagcaatct tttttacttg gattttaaaa
     8581 cttggtatgg actttttatt tataattcgc ggcatgaaaa tctcaggtac ccccatttcc
     8641 tggaaatctt ttttgctttt tgagttgtta caagctccgc ttatcaccat aagcagctta
     8701 ttatcccttt atcgttttct ccgaatgaaa aaacaaggtt ctctattcta atgaataaaa
     8761 aaacggaaaa atctaaatta acccttatta ttgccgaaaa accaagtgta gccgccgact
     8821 tagtaaaagc cctcggagca aaaaacttca agaaagaaaa aacccatttt gaaagcgaca
     8881 caacgattgt ttcttgggct attggtcacc tagtaacaat tgccgatcct aaaaacatgg
     8941 atgaacgtta caaatcttgg gatatgagca ctcttcctat aatcccagat caatttgtca
     9001 tcatgccaat tcctgagacg aaaagccagc tctccgctct aggaaagttc attcgaaata
     9061 aagacgtttc cacgattatt aatgcttgtg atgctggtcg tgaaggtgaa ttaatctttc
     9121 attatattat agaacatgaa aaaggaaaaa caggcctaaa aaataaagaa atcaaacgtc
     9181 tttggatgca aagtatgacc acttcagcaa tcaaagaagc ttttgaaaat ctacgtagtt
     9241 ctgaagaaat gcaaaagctt caagatgctg ccattagtcg ttctgaagca gactggctga
     9301 ttgggatcaa cggttctcgt ggtttaacag cttataaaag tagctttggt ggttttcaac
     9361 tcactccttg tggtcgagtc caaactccca cactggcgat gattgtcaaa agagaagatg
     9421 aacgaaatga atttgttcct caagattatt ggacaatcct tggccttttc aaaaacaata
     9481 atcaagaata cgaaggcaaa tggttccgta cagaagggaa agaaaacgtc caaagaatat
     9541 gggaacaagg gactgccgaa gccattgtta aaaaatgtga aaagaaaaca gggacggtta
     9601 cagagacaac aaaaccgtct tcacagaaaa gtcctccctt atatgatttg acctccctac
     9661 aaagagaagc caataatcgc tttggttttt cagcaaaaac aactttgcaa attgcacaag
     9721 ccctttacga acgccataag ttaacaacgt atccaagaac ggacagcaag tgcctaccag
     9781 aagattatat tagtaccgtt aaaaaagtac tagaatccat tactggacct cttgcagttc
     9841 atgctcaaaa cgtgctaaaa aataactggg tcaaaaaaga ccctaaggtc tttaacaata
     9901 agaacatttc agaccaccac gccattattc ctacaggaaa cgttccgaag agcctctctg
     9961 aagcagaaca aaaaatattc actttaatat gccaacgttt tatcagtgta ttcttccctc
    10021 ctgctaagta tttaaatacc acaagaatca ctgtcattga ggaagaaact tttattaccg
    10081 aagggaaagt tctcgaagat cctggtttca aagccgtcta tggtaaagat gctgataccg
    10141 agcaaacatt acctccatta ggaaaaagta acgaagcaaa aaacttggaa ataaaagtca
    10201 atcaagaagt cacaaaacca gctgctcgat acacagaaag ttcgttactt tctcttatgg
    10261 aaagcgctgg taagcttgta gaagacgatg atttgcgaga cgctcttaaa gaccgagggc
    10321 ttggtacacc agccactaga gcggccatta ttgaaaaact aatcacagat aaatatgttg
    10381 ttcgtgatgc aaaagaatta gttcctacca gtaaagcttt tgatttaatt cgtctacttt
    10441 cagcaatgaa tattgagcct cttacttctg cagaacttac tggcgaatgg gaacatcaac
    10501 ttgggctcat tgaaaagggc gaagaatcca gagaatcctt tatgaagggg attattggac
    10561 ttactcaaca aattgtttcc tccattaaat cctttaagga agaagacacc aaaaaagaag
    10621 cttcttttag cccagtcaat ggcattcgcg tatatgattc cgtttctcgt tacgaaacag
    10681 aagatggcat tatgatacga aaaatgctcg gtggccgact catcaatgaa gaagaagttc
    10741 aaacgttatt gttgaaaagg caaattggac ctctttctgg attcagatct aagcgaggag
    10801 cctcgttctc tgctgtaatt acaattaatg ataagaacaa agtggaattt ttatttgaca
    10861 atgcagataa tgatgtggaa gtgggtgaac aaatcggcat ttctcctatt gaccaaagcc
    10921 ctgtatttga ttctctcacc gcttatattt ctgaaagcgc tttacaaaaa gaaaaaacgg
    10981 gctttagact aagcaaaatg attcttggaa aagaaatttc tgttgaaaac atcaagaaga
    11041 tgctcgcagg agaaaaaagc gatttaatcc aaggatttcg ttctgcaaaa acaaaacgtc
    11101 tttttgatgc ttatctgact ctagataaaa atggaaaaat taaatttaat tttcctcctc
    11161 gagtggcttc tgcaaaaaaa acttttgcta gaaaaaaaac aacaaaagca agtgaatcct
    11221 ctgacgataa ttagtcttag ccctacaacc taaaaaactt aaacatgcct tattcagtaa
    11281 agacagaaag aacttttttt caaaaattcc tttttgggat tttactgtta caatttagtt
    11341 tagcctttgc taatacgacc acgacaaaaa tggctattcc tgaagaaatt cgtcctcttt
    11401 gggattccct ttcattaaaa gaaaaaatcg ctcaaatggt gatggtttat atgcctaatg
    11461 aagcctatat tacgacccat caatttggag ccattcttgt catgaaacct catctaaaag
    11521 atacaaacca attaaaagaa actattttca atgcaaatca aaatataaaa gttccactac
    11581 ttgtggctat tgatcaagag ggcggttttg tgaatcgctt atctaatgcc gatccgaaat
    11641 ggagtggatt acctagtgcg atagcgatga gatctatgcc cactcaagcc attgattcca
    11701 ttggcgaaat cacaggaaaa gctttatcga gtcttggtat caacttaaat ttagctccag
    11761 tccttgatcc cgctaccgat agccgaggaa aaaaaacctt tatcgaagaa agtagtcgtt
    11821 cttggggtaa tttagactct accaacatca atcatgttag agcttttgtt aaaggtcttg
    11881 cgaggtcaaa tgtattttgt gtcagtaaac atttccctgg ttacgacagc tggactaata
    11941 gcgatcacca aatttcaatg agttctgcac ctgattctca aatacaaaaa aacattcttc
    12001 catttaaaat gcttgttgaa gacgttcctt tcattatgat gtctagcgtt cgtttcagga
    12061 aaatatcaga gtctcctgcc gtttttgaaa aaaaattagt gaagcaagca aaagcactgc
    12121 atccaaatac gatcatttta actgatgact tgtggggaat gtcccttaga tcatggatta
    12181 gcggaagaaa gaatctaaaa cggaaaaact atcccaaggc tgattttgaa aaattgattc
    12241 atcaattttc atacatcctc acattttatt attttacttt cnnnnnnnnn natgtttatg
    12301 attacttatc ctgctaaagc agtggaaatg attcactatt tagaaagact agcgaagaaa
    12361 aatcgcaaat accgagaaca aattgaagaa tcagcattaa aaataattct tatgaaatat
    12421 aagtacggac ttttgaagcc gacaagtttt taatctcttt tgtaataccg ttttcaccta
    12481 ctttattgat agctaatttc aatatttccg ctgcagcaat agcatctccc aaagcgcggt
    12541 ggtgcttaaa atcagagcaa cctaacgact gagttaaaga gtgtaaacta tagcttggaa
    12601 aaccaggaaa aacttttcgt gcaagtttta cagtacataa acgtggtgga tcccacttac
    12661 aacaagctcg tgctagttcc actttaagaa aaccaaaatc aaaaggggca ttatgagcca
    12721 caaagatacg cccctcaagt agctcataaa ccgtttctgc tacatccata aaaggaggag
    12781 cagactccac catctcagaa tgaatacctg ttaaactttg aacaaaagga ggaataaatc
    12841 gaccaggata aactagactt tcatacgttt gaacgatctc agtaccatcc atcaaaacaa
    12901 ttccaatctc aatgatgcga tcatttttag gctgtccacc tgtcgtttct aaatcgacaa
    12961 tcgcaaaacg cattaagaaa gcaccttagt aataaaagcg cctatttctt gaatttcaga
    13021 taaagataca gaatgtggta acgaggcgta ttcatgaaaa tcaacggaat aaccttgctc
    13081 aattaatgca tcagaagtcc cttttcctaa agcaaaagga acaacttcat caactaatcc
    13141 atgtccccaa aaaataggcg ttttctgatt tgcttcactc ttttctgaat gcaatagaga
    13201 gagtgttgga gaatacgtgg ataaagcaat aataccacct agcctcttag gaaaacgaag
    13261 gcctagctct aatgcaataa caccaccttg actaaatcca gcaagtaaaa tacgagaagg
    13321 taaaatttct tcaccaattt ctcgctcgac ccaagaagca attctttccg cactagaatg
    13381 gattcccttt atatcaggat cacgcgcaat atccatggat gtgatatcat accaagcgcg
    13441 cattttgtag ccattattaa tagttactgg tacgatggga gcgtttggaa aaatgaattt
    13501 gatttttaaa ttctctggta aagacaaaga aggaatcaca tcttcaaaat catgcccatc
    13561 agcacctaat ccatgcatcc aaataatcac agcatcgtaa tcagaagctg tctttataat
    13621 tactgcatca ttcaaagttc aaaacctcta cctaaaaaaa ctaatataaa ttaattaaca
    13681 ggtacatcaa aatcaaccgt ttgttttcca gccttatttt cgatccttgc ttttatcgtt
    13741 ttcactttta tacgagaata gattggcaca gataaaagat aagaaacgcc agattcatca
    13801 ctaggttcga cttctgaatc aggttttgtc gtttgaaaaa cgggtttatt tccaccattc
    13861 catacagaat acgtatatgt tttaacggcg tgaggtttta attgttctgt tggcacacaa
    13921 aaatgaataa ttccaccttt cggaatttga cgtaaactgt ctaaaatttc ttcctctgga
    13981 gcaaaatcgg cctctaattc aagcctcttg tttttacgta atttttcata gctctcgtgc
    14041 attacataaa tattcacctt tgcttcttca ggaatagaag aaggccttaa atctcgcact
    14101 ttttcattat tttgataata ctcccaacgc ccatcatcgt gaagaatcac cgttgctcca
    14161 ttataggttg tagccatttg tgaagcaaaa cctaacccaa ttaaaaaaaa caaagaaaaa
    14221 atcaatctat tcatgaaaac actccaaaaa tacgaacgat ttcataaata tactattcat
    14281 atattctttt aagactttca aaaaactatt tttgatttat gtcacctctt aaaattcaat
    14341 ttttatgagc tttttaacta aaaatcccat ttatttgtcg ttcatattct tgaataccac
    14401 acctattcga aaaagtcctt ttttaccgtt gctcggttat gttaaaacct aataaagcac
    14461 accattttag agatgacttt atgacacata atattcgctt tgctccaagt ccgactggtt
    14521 atttgcacga aggtcattta ctttcagctc tttatgtatg ggcgatggca aaactaaaaa
    14581 actaccacat tcatttacgt atagaagatc atgatctaag tcgggctcga aacgagtata
    14641 tagattctat ttacgaagat ttagagtggt ttggttttga atgggattca gagagcaaac
    14701 aaagtgatcg tcatgaaatt tacgaaacag ctcttaaaaa gcttaacaaa gaaaatctga
    14761 tctatgggtg tcattgttca agaaaaagtc tatccatgga aaatccgagt tctcccgatg
    14821 gagaagtcat ctatcaaaaa cactgtctca acaaggctct accaattacc tctccaaata
    14881 caatacgctt aaaagtcgaa agcactttaa tccaatggaa tgatctacga ttaggctttt
    14941 ttgaagaaaa tccagcatta caatgtggcg attttgcact aaaagatcgt ctcggacaat
    15001 ggacatatca atttgcggta gtcattgatg atatggaaga aaacatagga atgatagtaa
    15061 gaggagaaga ccttagaaat tcgactgcga gacagatttt attaatgtct cttttaggca
    15121 aaacgactcc ccccctatat ttccatcact ccttattata cgatcctcaa acaggcttaa
    15181 aactttcaaa aaggcaacaa tcacaaagcc taagagcaga aagaatgaat gcgatagctc
    15241 ccgaagaaat acttggacgt ctctgtttta agcagcattt aataaaaaat aaatgttcta
    15301 tttctttaaa tgatgctatt ttattgtttt cgaagcaaat tgaaggatat cttcaacaaa
    15361 aataggtata ttcaaagcat gtccaaatcg atttcttctt cacaaatttc taaattacag
    15421 cttgaagttt tagattcctg tattcaaaat cgtttattaa attttcctgc acaaagtgtt
    15481 tttcaatctc cactaataaa agaaaatagt gatgttttta tcgataaatg gataaaaagt
    15541 ggaaagtctt tctctctaag ctcttttttt ggcaatgaat cattttcctt ttttgagcaa
    15601 attttaagaa gaaaacaaga agacttcgga gtttttgatt tatatcttgc caccggtttt
    15661 ttaaaatgga ccggcaataa aatggctcct gttttattaa tccccttaaa agtgaatctt
    15721 gaaaatttaa ctgttgaaat atctgattta gtaccgattg aaaacattcc cttacgagaa
    15781 caggcaaaaa acgaagttgc tcttcccaaa ataaatgatg ttcttataga tggtcgccca
    15841 gatcttgaaa aatacttcgg catggttgaa atggcaatca gcaaacaacc tctctggaag
    15901 ttttctcgac gaggtctatg tctttctttt tttagcacct cgcacttata tctttataaa
    15961 aatttcgatt tcatgcttaa ttctgaatcg tttaagtatt cagaaaattc tcttttttca
    16021 aaactattta gcgacgaagg ctttcgtata gcgacctcca tttttgatga aaaagaagcc
    16081 aatgacgcat accagccaac agatcgtgct tttctatacc ctactgattt tagtaccgta
    16141 aaagcgacag tggatgcttt aaatccaaaa aatgaggcct atgttatcca aacgcctcct
    16201 ggaacatttg cttttgaaac agctgcaaat attattgctg attctcttta caatcatcaa
    16261 aaagtacttg ttcttaccaa aagatctgtt actactgata aaataaaaaa aacactattt
    16321 ccagaatttc gttctactaa agggaccgat cgctctgaaa ttgttaatga attaaatcaa
    16381 attagaaatg atttagcgag ctactataag attgttaatt cagaaacaac aacaggtaaa
    16441 atttctataa atgatttatt cgaagaaaaa ataaataatc caaccattta taataagata
    16501 cctgactctg tttttaacgg aattgaaaga cttcaatatc aagaatacaa ggctgtaaaa
    16561 aagacaatag aaactcttgt ccatgaattt tctgatccca caacacaaaa ggcattagaa
    16621 acatttaaat cgtgtaatat aaaagtactt gataaagacc aacaaaacaa actagctcag
    16681 cttcttagtc caacaataga aagtattgat taccttactc cttttattga cgttttagaa
    16741 gctcaaacta tttttgatac tcccttaaca caagatacct tcgctcaaat tttttcaatt
    16801 attcataaat attttgataa agacactcca tcttatgagg ggtgggattt agggtcatct
    16861 gattgggata attacacgga aaatttatta caaattcctg aatctgggag tatttgggct
    16921 agatatcgcc gagatggatc tcttatttat acagaggctg ctattgatga aagtgtctta
    16981 gccattagag atgaatttgc agaaatcaca aagaactcat ttaaagcttt ttctgataat
    17041 tatcgttcta gaaaaaagaa gcttctttct ctatttaagg aacccaaaga aattgattct
    17101 gacgatgaat tgattttatt aattgatgaa ttaatagaac ttcagaatca caggaatttc
    17161 tataaaaatt cagcacccat ggcgaatcgt ctttttggaa aagactggaa atatgaaaaa
    17221 tcaaattggg ttcttttaca tgtaaagcaa aagtattttt atgaattaaa aaataaaata
    17281 aaagatcatg aaacagcctc gctaacattt aaaattctag aaaaacttca tcttctaaaa
    17341 aatctagatt ttgatataga agcgattgaa gctcaactta atgtcattca gaaaaataat
    17401 atcgaaattt caaagcttct ttctctcatt aatcctcttg aatcaattcc cttaatagaa
    17461 caggcgaatt tattaagaaa ctggatggat ggcttatccg atttagacat tcaagtgaaa
    17521 acaaatgctt ggattgatcg tttaaaacaa aaaaatcttc attctttaat cgactatatt
    17581 tttgacaaaa cagctccgaa agaagaatta ataccttctc ttgttcagta ttggtgtcaa
    17641 aatcaaatat ctgaattaac aaaagaaaaa aaagatctat ttaccgtttc tcctcagaag
    17701 cgatctcaaa cgagtaaaaa atatcgctct ctcattgatg aatactgtaa tgccaatttc
    17761 cgtttcatag aagagatgat aaaaaaattc cctgaaagtt tacaaatatc taatatttgt
    17821 gattattatg cagtttctca aaaagaacct caaaaatatg atcttgtttt gatccttgat
    17881 gcagaaatca tctcttctac agaatgtatt tcagctattt tatcatcaga aaaaatgatt
    17941 gtcatgggag atatagaaaa cccagaactt gagctattat ttacagacgg gtttgaaaac
    18001 acggatacat tacaaaaaat tagtgtatcc aagcaaaaga gtattttgag tcaagtctta
    18061 caaaaaggag caggttttag aactctttct ttttcaaggc tataccaaca tccagcatta
    18121 ttttatttta tcaacgaaaa cttttataaa aatacgattc agcaattccc tttacctcat
    18181 aaatccaatt ttaaaggatt agggttaaaa aaggtggctg atcctattca aaccatagtt
    18241 gaacaggctg ttcagcattt taaaacaaaa ccaacacaaa cactcggtat cattgcattt
    18301 tctgaaaatc aatgccagga aattcgaaaa gaacttgaag atataataga acaaaacgct
    18361 tttttgaaaa aagcattgta cggcacagat ctctctcgtt actgttttat taaaacacct
    18421 gaaagagccg taggtttata tcgagacacc atattagtat gtgcagagca ggatttaaat
    18481 tataatacct ctattactaa aggtaaaatc aaagtctctg cctctttagc aaaacaagaa
    18541 gtcttggtat ttatttcttc tatacaagaa ctttcaaatt caccaaaaaa cgatctttta
    18601 tctttatggt ttgaatatat acataaacat aagaccattg attattatca gatcaaagaa
    18661 gcgcaaagtc cactacttcg ctttgttcaa tctacttttg acaaaaatca aatttcttat
    18721 gaagtaggtt ggtctttaaa taatacatct atctgtcttt gtatttatga caataacaat
    18781 ccaaatcgtt ttttagcagc catagaagat gactcttatc ccaaatatct aaaagaatct
    18841 gtagaagaca ttgaatacat gcgtcctatg tgcctaggta atctcggttg gaagatgctt
    18901 tacatgtgga gcccaatatg gatatcgtct ttacaagacg aaatagacca tttactcgca
    18961 actatttcta ttgaacaaag tgttgctcca atacaacaag aaacaagtgc atcaacagaa
    19021 cctcaagccg attttgttat agaagagtca acaaagccag aaatttctgt tgatccctac
    19081 attataaaaa accctcagat tgaaggcaca gagcatcaca agcctatccc tgaattatct
    19141 cttaaatcaa ttatttcaca attactgttt tatatagata aagaaagccc aatccataat
    19201 gattgcttaa ggagaagagt tcttcaactt catcaactag atcgtgaagg gcctaaaatt
    19261 actcagatac tagataacgc cttaaagcaa ggtattcaag caaaagcatt tgttaaaaca
    19321 ggacctttct attattcctt aaaacaaaaa gaaataaagt tacgttaccg aggcgatctc
    19381 aaccctaatg aaagaaagct atcttacgtt tctccagagg aaagagcatt aataccatct
    19441 tctatggaca atgcttctgt tcgacagctt ttaggtttac ttgaataaaa actaacgaac
    19501 ttttataaca agagcattaa tgttactaac agaaagttct cttgctttat cttctgcttt
    19561 tgccttagaa ctccaaccac cgccccttaa cttatagaaa ggcgtgtcaa aaacgatttg
    19621 aattgcataa ccagtacttg cggaaagctg tgcctttcta cgttgtgcag catcaaaatc
    19681 agcaactgct tcaaactgaa gcatataata accatcacta ttcttgtctg attttgaagt
    19741 agatggagcc atctcaacaa ttttctcagt cgtagttctt aaagactcat ttaaaacagg
    19801 tctatattct tttcctctaa aaagagaatc taaatccgta ggatccgcaa aaagaaaaga
    19861 actaaataaa aatactaaaa agaatacttt atgcatgatt caataatagc tttttttatt
    19921 tataaaaaaa agctcccgtc ttcacgagag ctctttttta attaaagaat taaaaacgac
    19981 tattttaata ctttgccacc acgtctcaca ccccacattt ctgtttggtc gtgcttagct
    20041 gtttttttac ggccttgaat attaacataa gttgataact tcgcaatata agctcctgta
    20101 ccaaccttac gattatcttg tgatagcata ttccaagcta cataaacgta tttagggttt
    20161 tgtctacaat caccttttga tgatgtatcc gcaggataat ttccattaaa gaacggagca
    20221 tcacaacgga tattatgatt ggattcagca acaaaagagc ctaaattagt aaagtattgt
    20281 gttttaatag aaagcgttac ttcctccgct ttgacatcaa taaattcagg agctgttaaa
    20341 atattaccca tatctgtttt tacaatatgg cctaaagtat ttggattttt agcctgaatc
    20401 gtatccttac tatcataaat atcaacaggg atagcttcaa caactttacc ttcttccatg
    20461 tcttttttaa tcttatcagg atctggagtt cccaaagaaa tcgtaagaac ttcagactta
    20521 gcatcacctt caattggagc ccaaggagaa gcaacgtctg catcataatt cgtagggaag
    20581 tttccttgaa catcagaaat caagtcagac atgtctgcac ggaagcgaac atagtcaccc
    20641 gcacgtggag aaggtcttaa gttatcttta ttgttataaa gcatcactac agtatctctt
    20701 gcagcaagga catcacctga actagcggtc acatgtatat acttatttgc aggatcattt
    20761 cccacttctg tagctgactt taggtagtaa gtgaaagcac ttgacccata agaagttgcg
    20821 gctgtttgat ccttaattaa aacaggttca gaggcaagaa gagccacacg atcaaaatct
    20881 ggggatgtct tcacattaga aacacgacct ttcaagagaa tcggagctat acgatctgta
    20941 attagagtct gagaagctaa agccactgga actgttttgt ctaaataagt agaccaagac
    21001 gaaatgatcg tttgtggaga accaaccgtc tgaatctctt tagaaaattc taaaccacca
    21061 attcgaataa tactatcgca tacagaagtg gcttgatcac agtttgttcc tgcaacaatc
    21121 tttgctttat caatcttaat agaatctaat atatcaccag aaatagcttt tagatcccac
    21181 ttcacaaaaa ctgagtctgg tagagaatcc ttgtggaagg ctcgatgata atgaagaacc
    21241 aaagaatcag caataccatc tagatattct tgtgttgtag aaaaataagg tgttggaata
    21301 ggaagtgttc ttgccgatgg aacaccatgc ttatcaaata tttctgcaag aacagggaac
    21361 ggtactggag gatctttgaa atatagaggt gataatgtag cacttaacaa tgcggcatta
    21421 ggaccaacta aagaaagtgt agcggcaaca tctctatatt cggttaaaga acggaatgta
    21481 acaacagctt ctccatttac aatatctata gtcgcaggaa gagtctccac cttattggat
    21541 gtttgactac cactattcac tgcaaagtta caatcagaac aaaccttttc tgttatagga
    21601 ttaatggcca ctaatcgaac attataagca gatccaaccc acatagtgtc tgctagagtc
    21661 gttatcttat ttacagaaga atctgcaaaa atagtatctg caaaaacaat ttgaggagca
    21721 tagaaagtaa gagcggctac acttgtacga ccagccacct gaacagtatg agcctcagta
    21781 gaggcagtca ttgctgctaa aggaaccgta gcccaaagag tatcaatacc gctagattca
    21841 attgtacgag ggaaatcaga agcagctaaa atcacatcac tagaactatc agcatagaga
    21901 tttaagccct tagaagtagt taaactataa gtttgcccag cagcaccttc taggtctaat
    21961 tccaaattac cagtgcctgg gtcagccaca cttgaaacat agataggaat ctttgttcca
    22021 gccatagccg aacttacaaa tttccatgtt ggagaaggcg ataccgctgg atcaattgca
    22081 tcacgaacaa ctacatcaac agatcctgaa acacggaatg tgacataagt gctcttattt
    22141 cctataccca tcactagttt ataggttcca ggagataacc cttgcatgtt ttccacattg
    22201 attactggtg tatggcgatt agttaaatca ataccaccaa aatacactcc tgcagcagct
    22261 aaatcagcaa catcacccct aggaacaaca gagccatctc gacgagtcaa agaaatgtca
    22321 atcttttgcc cacaggtgct ttcaatagct gcaccacaac attctgtagt gccactagag
    22381 ccacccattg cggcagcaca agaaccatca ccagattctt gccagcaaag ttcatatcct
    22441 tttccttcag caggcttagt tacattatag cttatacctg ttttttgttg aatatacata
    22501 ttcgttttaa tacgaacgtt gctcatcgta gtacgacggt cacagaagaa aatatcaatc
    22561 gggtattctt cgccaacaac cagaggaaga tcatccttat ccttaatttg atctaaatta
    22621 acgtaaccag gtgccgctag atgagttcca cccaagtcta cagcgagttt accaccaata
    22681 aagacccaga tatcatcatc tccacggaaa ctaaaacgca agcctggctt atgtttgaaa
    22741 ttagcgtggg attcaaaaca gaaatgctgg ttacgtccac cagaaacgcc tgtagctttg
    22801 gaagtccaac ggccagtagc atcagttgta gaagcagaag ctagattttc tgtttctttt
    22861 gcatatttag gccaaccgtt aggagccata ttttcacagg accaacccca aacacatgga
    22921 ttagcttctg taggaaacat ttgccccata atcgtttcta aatcctttcc atcattaaaa
    22981 agcgttgaac aatcataacc gccactccaa ccagcaccat tacacattaa atccattttc
    23041 tgaacacctt ccacaggatc taattcacga agtagaggac caataaagac aggcccttct
    23101 gcagtacgtt tagtccgtgc ctttggagct ggtgttggag tccccgaata actaaaaata
    23161 ttagcacttg tcgtcgtttc gactggataa aaaccacctt gaacagttaa accagggcta
    23221 gtaaaataat cagaatcaaa ttcccagcgt ccatcatctg aacgagaaaa aggcatgtca
    23281 aaacatgaca tctcattgac tccgggagtt tcattaaaca attgattgaa aatagccgca
    23341 ctatggaagc aactcgttcc cgtacctgct tttaatactg gtttattagt gacagggtct
    23401 aaaagatctt caactattcc aggagtcaca ccaatacaag aattcacagc ggccaccgct
    23461 gtagcctgag gaataccctg agcccctact tggcaacctt ctccgccctc actccaacaa
    23521 gaaaaagaag ggtgtaatga agcgtctgta tcataaataa tagccgctaa attataagaa
    23581 cagcttccat ccaccatcgg atcaacatca taccaaccta atgaagcacc ttcatctggc
    23641 cataaattat catcaggaat aaaataaaga tcattgactc cataaagagc aaaataagtt
    23701 gccataggaa ttggatcgac aacaccatct tcatcccaaa gaccatttaa accaatcgca
    23761 tctgctttag ttgaatcatc atctcgatat attataactt gttctgtagg ggcgtcacta
    23821 aaccaaacat gatagtacca accacaacgt ttttcatcag cagacatagg tactccagta
    23881 gcacctccat ccatactaat catgggaaca gcagacatcc attcctcttt ttctggagga
    23941 atcaacacat ataagtattt tgcatttggc gggtctgtgc taataaccgt tttccctgct
    24001 tctgatggat tatcatatat atataaatca gtccctgcac caaaatcact gcatgtaaaa
    24061 gcagcacttg cttctggagc ttgcccagca aagtcataaa ctgttctaga aatccaattc
    24121 gctgggtgac tcacacccgt attattaaaa acaaagtttt tttcataagt ttgacttgat
    24181 gtaggtacat ttggacctaa tgcaatatca caccaacccg tagtggcatt acactgaggc
    24241 gtgacatttc catatgcccc tcctgcatag atgtatacac ttgcccaagt agttggtttc
    24301 tgaaaatgaa ccgtacctga acaactcatt gcataagctc cacccatacc cataaggagg
    24361 agcaccaaac caaacaccac cgttttaatc gatgtattat tttgtttcat agtttctctt
    24421 ttcataaaaa ttctctccaa aaccaataaa caaagttacc gtcaaagtct tacggaaaca
    24481 atgtcaccac gcctaaaatc tatgctattc taaataaaaa tgctactttt ttctttagta
    24541 aaacgttttt tttcatttac tagtgagcac aatcgtctat ttctgctttt ttggccaaaa
    24601 acagtaaaaa aaagggtttt taggtagttt ttacaatgta attttaattt ataatagaat
    24661 cgagacaata ttttataata aaaaaatatc tttagtcttt ggataaatat tttttggctt
    24721 caagaggcat ccaatgcaag atctagaaaa aacaattgta accaaaagtc aaacgattcg
    24781 cttacctgaa ttagaaacta gagcacattt gattattctc taccctagag aacttttccg
    24841 acaaatccct ctaaaaacgg gaaaaaacat acttggccgc ggaattgaag ccgatattcg
    24901 tctggatgat ggccttgttt ctagaagaca ttgtgaaatc tcttgggacg gaaaaaccgt
    24961 tcgtattcga aacctaaaca gcaccaatgg tatttttatt gatggccaag aagttgaaga
    25021 agccgtttta gattcagaaa accgcattca aattggaaaa atggtcttaa agatcgattt
    25081 taaggatgct agtgaagaag cttttgaccg agaattattt gaagccgcta caacagatcc
    25141 actcacaaaa atttctaatc gacgaacatt tttagatcgt tgcagcggag aatgcgccct
    25201 cgcccgtcga aatgattatt atgtgcacac ccttctcata gacgtagacc atttcaagag
    25261 agtcaacgac caatggggac atcaagcggg agatatggtt ttaaaagaga ttgcgcggct
    25321 attaaaggat gagaaacgtg aaggagatct tttagccaga tacggaggag aagagtttat
    25381 tcttttattt tctggaatcg gtcttgaaga tgctttgaga agtgccgaac ggttacgcct
    25441 tgcaatagaa aagcaaattt ttgtctggaa cgacataaca attcctgtaa cgatttctct
    25501 tggacttgcc tcaagaaaag ggaaagcgat tcctaaaata gaagaaatga tctctgaagc
    25561 agataagttc ttatacatag cgaaagataa tggaaggaat caagtcgtcg ttgcctctta
    25621 aattataaga gtttcactat
//
LOCUS       DAOOBB010000006        24280 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_2710_length_24280_cov_5.205655, whole genome shotgun sequence.
ACCESSION   DAOOBB010000006 DAOOBB010000000
VERSION     DAOOBB010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 24280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24280
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2710_length_24280_cov_5.205655"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PLT31_00670"
     CDS             <1..440
                     /locus_tag="PLT31_00670"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPW93676.1"
                     /translation="RPTVLIGGPVGGIWYAPSPLSYTAELVRDAGGHYIWADQKTNKW
                     FNFSLEEAILQGKNADIWIHPSNFKTPDEVLQNERRVDLIKAWKEKRVYQNDKRVGPV
                     GGNDFYEEAVVRPDLVLEDLIKVFYPNFFEDYSMRWYRNIYIF"
     gene            444..1259
                     /locus_tag="PLT31_00675"
     CDS             444..1259
                     /locus_tag="PLT31_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPW93677.1"
                     /translation="MKANSGIGDWISAAYEQGIPFLQQIPREAADFILLNASIQEYDA
                     GDVIITGGDSNNESFCVLQSGRAQICGRILPDGHYNVLAYLETGACFGEMSIICNDTT
                     SNTVIAAEDNCTVLHLARDIFVDFLERNPSIMVYLYKVLADRLRAKNQAFDEFESLAL
                     LASAKVLPFIDFAQTLEKSRVTGTVMFECKGESGFIAFQEGRICCAKSGKLTGPDALE
                     KMLSWGDSTLFKLDTHLMPDIVNICQASDTTSLILDALRNIDEKESKKQLNEE"
     gene            1260..2285
                     /gene="purM"
                     /locus_tag="PLT31_00680"
     CDS             1260..2285
                     /gene="purM"
                     /locus_tag="PLT31_00680"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HPW93678.1"
                     /translation="MNYADAGVSLARADEAMVGVKKSVRSTFNAGVLGDVGNFGGLFS
                     LNHLNLKDPVLVSSVDGVGTKLKVHIELGSHDLPGQDIVNHCCDDILVQGARPLFFLD
                     YVATGRLEPGVMDSLVAGMSKACRENDCVLIGGETAEMPGFYGPGDYDISGTIVGVVE
                     RDRIIDGKDIKPGTVILGLPSTGLHTNGYSLARKALFDVAGYKIDSLVEGMDKTVGEA
                     LAVPHRSYYKSLIDLATNQKLQGLVHITGSGFQGNIPRILPDNVDVVIDRKAWEVPMI
                     FKLIQEAGSVEKDEMYSTFNMGLGMLLFVDPADRSFVENHLKEKNEVFYHVGEVIAGS
                     KKVQFRD"
     gene            2306..4696
                     /locus_tag="PLT31_00685"
     CDS             2306..4696
                     /locus_tag="PLT31_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HPW93679.1"
                     /translation="MIQKGLRIISLCIVGAMISCSNSVEPDSEDDDKSSSSIVRSSSS
                     VRSSSSTNSEQSSSSISIAIDLGSVTDLKVSKSISDNWLLTWVYTPGTVEEKGFQIDF
                     YNEKENEWQAYDTVSVGNYRYVFNDTLSGTFRVSAFNGQRRSTFSNEVIIYYKTAAAD
                     PTEKVNLGAVAQLSVSKNTSDLWLLNWVYTPGTVEEKGFQIDIYNDTKNAWEPFDTVA
                     TGTYRYLFNKEVLGVYRVSAFNEQGQSTFSNEVIIYNKASIINPTEKLDLGSVAQLTV
                     SKNASDLSLLTWIYTPGTDVEKGFQIDKYNESANVWERHDTVGVGTYRYVFNDTISGA
                     FRVSAYNEQGQSTFSNEVIIYNKASIIDPTEKLNLGSVAQLTVSKNASDLSLLTWIYT
                     PGTDVEKGFQIDKYNESANVWERHDTVGVGTYRYVFNDTISGAFRVSAYNEQGQSTFS
                     NEVSITNKTPMIEIDLGSVMNLSMTKNSSGSWILTWKHVQGTDVENGFEIDLFNDATN
                     TWESFDSVGVGTYRYVFGSNTPGVYRVSAFNEKGRSSYSNELEVQNAVTDLIHLQKPT
                     NLVGIRLAPSIWQLSWNYTAIVERRENGFIIEKYDPTLLKWIVWDTVDTDVKRIVLDS
                     VVGAVDLSLGSYRVAAFDSLGRSDYSNDIQVNIQTEYSPDIIFVPPTDMVPNASNSAY
                     LELLIPTNPVNKSVEFSPYTASLSYEALWVIEDTEESPVSFSFSKNSVTHSVDPATNA
                     LCDSYARIRLVWTDINGVVDYSEWSVPTGSKPGTRGSGLIDLKESPNCPELIKVGQ"
     gene            complement(4769..6328)
                     /gene="murJ"
                     /locus_tag="PLT31_00690"
     CDS             complement(4769..6328)
                     /gene="murJ"
                     /locus_tag="PLT31_00690"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HPW93680.1"
                     /translation="MNKAAIIIAISMLASRVLGIFREMLLARAAGVSTEKNALDLAFM
                     IPDILNHIVSTGFLSIIFIPIFTGYLVQKKEEAAWRFFSNILNTLGLFLLFLIIPSFI
                     WMRELIELFTVADISPELLERATYFGRLILPAQLFIFAGTFLVAVQHTRKQFLIPSLT
                     GVIYNAAIIVGGILFSDFGLEGFAWGVPIGAFVGFFVLQILGAYRGKIQYFFYINPKH
                     PDIIRYLKMMLPMSLGVGSMFALEFVIRSFGGHFGSSGISSLNYAYRIMYTLVAVFGF
                     SVGVASYPDMARLVKENHIDELNKKIWKSISRMFCILIPAVVAVWALSFPAVRILFER
                     GAFTRETTEAISEILRWYLPVSLGLCLQAVLVRSFYAAEKMWTPTLLNTGIFALTIPA
                     YLFLGPVLGIKSVPIVGASGAIAQVIAMIFLWAKNNGTNGMKEALSNIGRAVVSLALM
                     IVVALFLDSFSNEFIRNTSLLTLISYSIIVGVFLFTLTLVIQKSLGSSDAKDILNELL
                     GKVLRKLHLKC"
     gene            6618..7634
                     /locus_tag="PLT31_00695"
     CDS             6618..7634
                     /locus_tag="PLT31_00695"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPW93681.1"
                     /translation="MRILFIGGTGTISMAISKKLLADGHTLYLINRGTRNDLLEGDIH
                     SIVVSDINNEAEVKEKIKDLTFDVVADFIAFVPAQVERDYRLFKNKTKQYIFISSASA
                     YQKPLSDYRINEGTPLSNPYWEYSRNKIACETFLMDCYRNEGFPVTIVRPSHTYDERS
                     IPLGVHGKNGSYQVIDRMLQGKPVIIHGDGSSLWTLTHNSDFAKGFIGLLGNIHAIGE
                     SVQITSDETLTWNQIYQTIAQKLGVKLNAYYVSSSFLAACSDMDLEGSLIGDKANSVV
                     FDNTKLKRLVPGLTMTKRFDQGIEETLQYVLSHKECQKKDPEFDAWCDRIIDSLEKIK
                     NKGE"
     gene            8389..10164
                     /locus_tag="PLT31_00700"
     CDS             8389..10164
                     /locus_tag="PLT31_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW93682.1"
                     /translation="MALLSVQNLKLTFGGPPLLDGVSFDVEPMERICLIGRNGEGKST
                     LFKILSQEMASDSGEVISKTGLRMARLIQEIPAHMDGTVRSIVFGNEEHEGYSAKEAI
                     LGKTGIDPELPYDSLSGGQKRRVLFAKALALEPDILLLDEPTNHLDIPSIQWLEGILS
                     RYNGAILFISHDRAFVRRLATRILELDRGQIRSWNCTYDQFVNYQMGQIEDEERANKL
                     FDKRLAEEEVWIRKGIKARRTRNEGRVRALIRMREERKERRSRTGQVSMQITEADRSG
                     RLVARLDNVSYSYEENPLIQPFSTEISRGDRIGIVGENGSGKTTLLRLMLGELTPITG
                     DVRLGTNLEIAYFDQMRNRLREDKSLVENIGDGQEYIELQGKKKHVLSYLQDFLFSAD
                     RARGPISALSGGEKNRLLLASLFSRPSNVLVLDEPTNDLDMETLDLLADLLGEYKGTV
                     LVVSHDRAFLDSIATTIIGIEENGMIYECVGGYSDYEKSKKRRETEITENVSLKQSKE
                     ISENRISTTKKKRSYKEEMEYNALPDKIEQLEAEIEKLQIELTDASVYTNPEKLIKIQ
                     TELAEKESLLLKFYERYEELDSIGS"
     gene            complement(10173..11234)
                     /locus_tag="PLT31_00705"
     CDS             complement(10173..11234)
                     /locus_tag="PLT31_00705"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPW93683.1"
                     /translation="MNLVLNLTEQCNLRCSYCYYNHLQEERFCSMNLNILERAIAYGF
                     QRSLELGDSFLNITFFGGEPLLKKDLILKGVDIAKKYKPEKMKLQFAVNTNGTLINSF
                     WLDFFKKENFYLYLSLDGPAEIHDKQRYTIRKHGTFAFIEPHLKTIRQLNTTILRVIT
                     HDHIQGLSDSIEWIVNQGFTKVTTAVDFDGKWTSDHFNELKKEYEKIASYWVSLKKKK
                     HPLYLGTIQDKIRLELESTSYKKKTCSILERIVAVSANGTLFPCTRFVSSKPDALYKI
                     GTVYTGIDESKTQEIKSFLKSDKETCKECELKSRCVGNECGCITFSTTNTLSKVSPEV
                     CTHERMLALICDEAAMQLI"
     gene            complement(11244..11747)
                     /locus_tag="PLT31_00710"
     CDS             complement(11244..11747)
                     /locus_tag="PLT31_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93684.1"
                     /translation="MNIKIKHFQKKKSYPNNGKTKLLAALGLAASLGIAGCDITEQPL
                     SGDPAMPESSSSEEPPLGGEIIMPESSSSTEVPESSSSIKTPIPIGGVPVDPIQPLAG
                     TIIEVYRFESSSSVSSSSSEEPPLAGGVQIIDSSSSTDSPKAGIVEIPESSSSTNASL
                     DDESLIL"
     gene            complement(11929..14409)
                     /locus_tag="PLT31_00715"
     CDS             complement(11929..14409)
                     /locus_tag="PLT31_00715"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HPW93685.1"
                     /translation="MAKVTSKNDSSASSVLDTSAEAFKKAFTDHIHHTLARNEKTVAE
                     HEKFLAVAYAVRDRLIDRWIQTQETYYNNDVKRVYYLSLEFLIGRTLGNSVLNLDVEG
                     AVNEALHELSMDLEELREQEVDAGLGNGGLGRLAACFLDSMATLELPATGMGIRYEYG
                     MFHQKIQDGQQEEHPDNWLRLPNPWEIARPANALRIPFYGYVVSWVDEKGKLNNRWET
                     KDYVLALPYDTPIPGFKNNTVNNLRLWSAKSTDNFGLAYFNNGDYLAAVHDMELSETI
                     SKVLYPNDSSMNGKELRLKQQYFLCSASLQDIVNRFKKTHNNDWALFPEKVVIQLNDT
                     HPAISIAELMRILVDIENMEWDEAWDIVTKTFAYTNHTLMPEALEKWPVSLFERLLPR
                     HMQIIYEINARFLRIVSMKWPGDTKRLGRMSIIEEGSEKMVRMAFLSIIGSFAVNGVA
                     ALHSNLLKSHLFLDFYELWPEKFNNKTNGVTPRRWVRKANTALSALVTSKIGDSWIRQ
                     LDDLKKLEPFAKDASFQKAFMDVKFENKKRLALYLKQTQNVDLDVNTLFDVQVKRIHE
                     YKRQLLNILHAIHLYIQIKDEKEIVPRTIMIGGKAAPGYWMAKQIIRLTTAVGNIINN
                     DPDCKGKLSVIFLENYRVSFAEKIIPAADLSEQISTAGTEASGTGNMKFALNGALTIG
                     TLDGANVEMLEEVGKDNIFIFGLTVEQVQELLAKGYRPKDFYESNDDLRRVLDLIQSG
                     FFSPERPDLFKHIVDKLLSNDSYLLLADFADYVKTQKEVEKLYLDKKAWAEKAILNVA
                     RMGKFSSDRTINQYAKEIWNAKACPIKL"
     gene            14700..15371
                     /locus_tag="PLT31_00720"
     CDS             14700..15371
                     /locus_tag="PLT31_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HPW93686.1"
                     /translation="MKVVLLGSSGLVGGFCRDILALEPKVTHVHCLVRSIDSSFKESD
                     KVHYSVVDFTNLTPYKSFFEADAVICCLGTTIKKAGNRKAFEAVDLQMPLAAGALAKA
                     SGVKHFLAISAMGANPHSWIHYNRTKGLMEEGLSMMGFESLTLVRPSLLLGDRVEKRR
                     AEDLFKKFLSKRLYSLPAFFRPVHAQSVASAMILSLLQPPEGKRIVYNRKITELELLN
                     FRRTP"
     gene            complement(15348..16529)
                     /locus_tag="PLT31_00725"
     CDS             complement(15348..16529)
                     /locus_tag="PLT31_00725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPW93687.1"
                     /translation="MKNKEYDVIVIGAGPGGSVIARRLSQYGHSVLLIEKREQIGFPV
                     RCGEASTTLEDIESYGPIDEDCIETIIDGLFVYGPGGTTVESIKKDTGIMLNREKFDP
                     CLAKLAEQDGVELVTNARAESVSAVIGKFREVSIVTPQGTEKIKGRMIVGADGMESLV
                     GRMAGLKCLHNVNSTCTGVDIEVEGILTDPRYLTFWQGHDFINDGYIWSFPKVKSNVT
                     NFGAGFLIPKSKDLSIQDITLQWLYKLFPEAKVNKITGGLISVSGNLKEYVTDRLILV
                     GDAAHHTNPLTGGGIAAAMRAAKEGAEVIHNGLIKDDLSKNFLKNYEERCSIVFGKKH
                     QLLLKFRKFLLGQKREDQIEFYKVIKTFVKSNFSYLALIKCPIITLKCLYRYLRSSSK
                     I"
     gene            complement(16519..16713)
                     /locus_tag="PLT31_00730"
     CDS             complement(16519..16713)
                     /locus_tag="PLT31_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPW93688.1"
                     /translation="MKKLSHDRKNCLECAGCVGVCPTMALDMFGLDLQINHDKCNRCG
                     ICVRACPAGVLNWIEKPDEK"
     gene            complement(16897..17769)
                     /locus_tag="PLT31_00735"
     CDS             complement(16897..17769)
                     /locus_tag="PLT31_00735"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPW93689.1"
                     /translation="MIEEKILIVDDNPAILQKTEELFTQVGYKVAFCSSGEDALQFLE
                     TERVDLVLLDINMPKLNGYEVCLRIRQQYALDDLPIIFLTGREDFESITRGFHAGASD
                     FVSKASVPEILLARVNVHIRLARTLNFLRDISLTDDLTQCYNRRHAMYTLKEWFARSK
                     RYGTPFSLIYFDLNGLKRVNDKYGHQAGDLLLRSVVAAIKKLLRESDMLFRMGGDEFM
                     VLCPDTDKRGADICAERMQDAVKAVTIIDQNISFAYGISHSVDLYKDMDEMLSTADKL
                     MYQCKKEMKKKEEK"
     gene            complement(18085..18906)
                     /locus_tag="PLT31_00740"
     CDS             complement(18085..18906)
                     /locus_tag="PLT31_00740"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HPW93690.1"
                     /translation="MNLNIGRIPFLVCAPFFYNFFTKNENFPNFLFIDGTPRAHNEAL
                     NKNIIDLAPASSITFAKNPELFLLSPKLCTSCRFEVQSVKLFSQRPLLELNQKNIHLT
                     NQSDTSIALLDILLSLRFHITPTYIRNRAYDPKEDVARLLIGDQALLETYENHFSYSY
                     DLASLWEEWQHLPFVFGAWIIHKNALVKEKKDLLDLFLTATEENVNSFRKSPKTAIET
                     WLKNYPVQLPARTIFSYYDSLDYSFTSERKESLELFFSLCHQRGIIPTATKLKFI"
     gene            18978..19286
                     /locus_tag="PLT31_00745"
     CDS             18978..19286
                     /locus_tag="PLT31_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93691.1"
                     /translation="MPKFHVKKTSLGDDQTSLIFKGKVIEGPINRGMILEIPITEAAV
                     ISVKVYDIVHFEKQKDEMKKVGLVVDFYGAPDDLDVIMGLNIVDEELNVVEAPVGSEV
                     "
     gene            complement(19353..20981)
                     /locus_tag="PLT31_00750"
     CDS             complement(19353..20981)
                     /locus_tag="PLT31_00750"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPW93692.1"
                     /translation="MTSLLFYSEIVFWISLCLIIHCYLVFPITLPFVSEFFKRKHKSF
                     SGKFQPKVSILISAFNEEGIIEKKIQNLLELDYPKEKLEILIGDDGSIDRTAEIILKY
                     KEQGIRLIQAEKNAGKAAMLNRLQKEAQGEILLFCDANTLFFPNVVRKIVAPFEDPKV
                     GCSCGHLILSDYSGSILGQGESSYWDLESEIKRFEGVLDRLIGGNGALYAIRNELYTP
                     LPTNKSVMDDFFITVKVLQKGFFSTFVSTAIGTEQTSKESIGEFRRKVRIGRANFNFL
                     FSYLALLNPFRPLLAYLFFSHKLLRWFTPHLGIFLILSNLVLILTKKPIYIGSFVFFT
                     LLILIGALRLIPSAFYFLSMNTAILKGFFLSFLREKGGGWQREARTDDQANTVSIKGI
                     SFLICFILSLSVAVPNANALLTMDLSVGALTTAPKPDRANISLGGHLWLPIDQMIFIG
                     AGGYYQEIDKTIFIPVTGSVWIRLPIGKQVLPLIIADYGYAVGSDHQFVWSAGLGIDI
                     KNGDYSSLILMAGYQKLKKKDDYFYIRAGFLIEI"
     gene            complement(20978..21490)
                     /locus_tag="PLT31_00755"
     CDS             complement(20978..21490)
                     /locus_tag="PLT31_00755"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HPW93693.1"
                     /translation="MRSMLVSQNSVCKFLFKGRLFLCFLLLATLTAFSKENPVGVKWE
                     PYDQALKNAVVDKKMVFTYLSANWCTSCRGMDKNVFKDPFIVNILNTKFHPVKLDVNS
                     EEEIVCDGRIKTVERCFFDIWKLNGVPSFVLIAPKGLTILTLTQSLQSSEMRFLLQQF
                     LNKEKEWIQE"
     gene            complement(21487..21750)
                     /locus_tag="PLT31_00760"
     CDS             complement(21487..21750)
                     /locus_tag="PLT31_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93694.1"
                     /translation="MLGCESLSPTISEEFVNAYIELRIVSLELGDVNPNARIKRTEIL
                     KKYNYTTESFNQESERIKTDPKLWLLFQQKVVSIVDSLPETRK"
     gene            complement(21800..22726)
                     /gene="ftsY"
                     /locus_tag="PLT31_00765"
     CDS             complement(21800..22726)
                     /gene="ftsY"
                     /locus_tag="PLT31_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HPW93695.1"
                     /translation="MGLLSVIKGGLTKTRNALLSELKGIVGAGKITDEMLENLEEHLI
                     KADVGVEAAFLLTDALRENALGKSLTTDEVLNIMRAEAERLLSDPPPFELKGNPHVIL
                     VIGVNGAGKTTTIGKLASRFASEGKKVLIAAGDTFRAAAIEQIETWAERSQAEFVKHQ
                     EGSDPAAVAYDACEAAKARGCDVVLIDTAGRLHNKDHLMEELRKVVRVIKKLDPSYPH
                     DLWLVIDGNTGQNTINQTKVFHQSFPLTGLVITKLDGTAKGGSVLSISSALQIPIRWI
                     GMGEQIHQLVPFNKKEYIDGLFENALLNDEEN"
     gene            complement(22728..23315)
                     /locus_tag="PLT31_00770"
     CDS             complement(22728..23315)
                     /locus_tag="PLT31_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HPW93696.1"
                     /translation="MEKKDCEKENETKTPDFLKTAEEYLAKERRLFLWGAIDDESAEK
                     TVKRLLYLDSLETKDIILFINSPGGVISSGLAIYDCMQAIKSDVVTVCCGQAASMGAV
                     LLTAGAKKKRFAWPNARIMIHQPLIHGEMVGPASDIQIQAEEMLRIRSVTGQILAKHS
                     GKNLKDIDRDTERDNFMSAEEAAAYGLVDAVKSIL"
     gene            23380..24159
                     /locus_tag="PLT31_00775"
     CDS             23380..24159
                     /locus_tag="PLT31_00775"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HPW93697.1"
                     /translation="MNNAGSPLIAGIISQTNLVQAESELGGVIHSRLLLCPVLEIRYD
                     LFQNVKDWPLLAGRVRALHPTAKIIGTIRLKRDGGALEDSIVKERLWLWESILSADVV
                     PLWIDLELFALGPEALLLKEMASAKGSRLFISMHDFLKIPTTPELELLAKRAIDFGAC
                     GVKVAAMSHSPGDARPLYDFLRRIESQFELRAAFAMGVSGSVSRVWSLAMGANLTYGS
                     ISDVLVPGLLSAKKMTQAIQFLRECKTEEQMSLFLEEIRES"
BASE COUNT         7747 a         4088 c         4711 g         7734 t
ORIGIN      
        1 ttaggccaac agtcttaatt ggtggtcctg tgggaggcat ttggtatgct ccttctcctt
       61 taagttatac agctgaacta gtaagagatg caggaggaca ttatatttgg gcagatcaaa
      121 aaacaaataa atggtttaac ttttctttag aagaagcgat tttacaaggt aaaaatgctg
      181 atatatggat tcatccatct aattttaaaa ctcctgatga agtcttacaa aacgaacgac
      241 gtgtggattt aataaaagca tggaaggaaa aaagagtgta tcagaatgat aagcgcgtag
      301 gaccagtagg tggtaacgat ttttatgaag aagctgtggt tcgacctgat ttagtgcttg
      361 aagatttgat aaaagtgttt tatcccaact tttttgaaga ctattctatg agatggtata
      421 gaaatattta tattttctaa catatgaagg caaactcagg tattggtgat tggatttcag
      481 ctgcttatga gcagggaatc ccttttttac agcaaattcc tcgtgaagct gcagatttta
      541 ttttgttaaa cgcaagtata caagaatacg atgcgggtga tgtgatcatt actggtggcg
      601 attcgaataa tgaaagtttt tgcgttttac aaagtggccg tgctcagatt tgtggtcgta
      661 ttcttcctga tgggcactat aacgttttag catatttgga aacaggtgct tgttttggtg
      721 aaatgtctat tatttgcaat gacactacaa gcaatacggt tatcgctgct gaagataact
      781 gcacggttct tcatttggct agagatattt ttgttgattt tctagaacgt aatccaagca
      841 ttatggttta tttgtacaaa gtacttgctg atcgcttacg tgctaaaaat caagcctttg
      901 atgaatttga aagtcttgca cttttggctt cggcaaaagt tttaccattt atagattttg
      961 ctcagactct tgaaaaaagc cgagtcactg gtactgtgat gtttgagtgt aagggtgaat
     1021 ctggttttat tgcttttcaa gaagggcgta tatgctgtgc taaaagcggt aagttgacag
     1081 ggccagatgc tctcgaaaaa atgttgtctt ggggcgattc cacgttattt aagttagata
     1141 cgcatttaat gccagacatt gtgaatattt gtcaggcttc agatacgact agtttaatat
     1201 tggatgcgct tcgtaatatt gatgaaaaag aaagtaaaaa acagttgaat gaggaataaa
     1261 tgaattacgc agatgcaggt gtatctttag cgcgcgctga tgaagcgatg gttggtgtta
     1321 aaaaatcagt ccgttctacc tttaatgcag gtgtgcttgg agatgtaggt aattttggtg
     1381 gtctattttc cttaaatcac ttgaatttaa aagatccagt tttagtaagt tcagtagatg
     1441 gtgttggtac aaaattaaaa gtgcatattg aacttgggtc tcatgattta cctgggcaag
     1501 atattgtcaa tcactgctgt gatgatattc tcgttcaagg cgctcgtcct ttatttttct
     1561 tggactatgt cgctactggt cgtttagaac caggagtcat ggatagcctt gtcgctggca
     1621 tgagtaaagc ttgtcgagaa aatgattgtg ttttgattgg tggtgaaaca gcagaaatgc
     1681 ctggtttcta cggtccagga gattatgata tctctggaac cattgttggt gtcgtggaac
     1741 gcgaccgtat tattgacgga aaggatatta aaccaggaac ggtcatttta ggtttgcctt
     1801 caacaggttt acataccaat ggctattctt tagcgcgtaa ggctcttttt gatgtggctg
     1861 gctataaaat agattcgctt gtagaaggca tggataaaac agtcggagaa gccttagccg
     1921 ttcctcatcg tagctattat aaaagtttga ttgatcttgc tactaatcag aagctacaag
     1981 gcttagttca tattactgga tctggtttcc aaggaaatat tcctcgtatc cttcctgaca
     2041 atgtggatgt tgtaattgac agaaaagcat gggaagtccc tatgatcttt aagttgattc
     2101 aagaagctgg ttcagttgag aaagatgaaa tgtattcgac tttcaatatg ggacttggaa
     2161 tgcttttatt tgtagatcca gcagatcgtt cctttgtaga aaatcatttg aaagaaaaga
     2221 acgaagtctt ttaccatgta ggtgaagtca tcgcaggttc taaaaaagtt cagtttagag
     2281 attaattttt tccctggagg gacttatgat tcaaaaagga ttacggataa tatcattgtg
     2341 cattgttggc gctatgatct cgtgttcaaa ttcggtggaa ccagattctg aagatgatga
     2401 taaatcttca agcagtattg ttcgaagttc ttcttctgtt cgatcatcaa gttctacgaa
     2461 ttctgagcaa tcctcttctt ctatttcaat tgccattgat ttaggttcag tgactgattt
     2521 aaaagtttcg aaaagcattt cggataattg gctcttgact tgggtttata ctcctggaac
     2581 agttgaagaa aaagggtttc aaatcgattt ttataatgaa aaagaaaatg aatggcaggc
     2641 ttatgatacg gtgtctgtag ggaattatcg ttacgtcttt aatgacacgc tttctggtac
     2701 tttccgagtc agtgctttta atggacaaag acgatccaca ttcagtaatg aggttattat
     2761 ttattataag actgcagctg ctgatcctac tgaaaaagtg aatttggggg ctgtggcaca
     2821 attaagtgtt tcaaaaaaca cttcggattt atggcttttg aattgggttt atactcctgg
     2881 aacggttgaa gaaaaagggt ttcaaatcga tatttataat gatacgaaaa atgcttggga
     2941 accatttgat acagtagcca ctggaactta tcgctatctt tttaataaag aagttctagg
     3001 ggtttatcga gtcagtgctt ttaatgaaca aggacaatct acttttagta atgaagtgat
     3061 tatttataat aaagcttcta tcattaatcc tacggaaaaa ctggatttgg ggtctgtagc
     3121 tcaattaacc gtttcaaaaa atgcttcgga tttatcgctc ttgacttgga tttatactcc
     3181 aggtacagat gtagaaaaag gttttcagat agataaatat aatgaaagtg caaacgtatg
     3241 ggaacgacat gatacagtag gcgttggtac ttatcgctat gtatttaatg acaccatttc
     3301 tggtgctttc cgagtcagcg cttataatga acaaggacaa tctactttta gtaatgaagt
     3361 gattatctat aataaagctt ctatcattga tccaacagaa aaattgaatt tggggtctgt
     3421 ggctcaatta acagtttcaa aaaatgcttc ggatttatcg ctcttgactt ggatttatac
     3481 tccaggtaca gatgtagaaa aaggttttca gatagataaa tataatgaaa gtgcaaacgt
     3541 atgggaacga catgatacag taggcgttgg tacttatcgc tatgtattta atgacaccat
     3601 ttctggtgct ttccgagtca gcgcttataa tgaacaagga caatctactt ttagtaatga
     3661 agtttctatc actaacaaaa ctccaatgat tgaaatagat ttaggttctg tgatgaattt
     3721 aagcatgact aaaaatagtt ctggttcttg gattttgact tggaaacatg ttcaaggtac
     3781 agatgtagaa aatggctttg aaatagatct ttttaatgac gcgactaata cttgggaatc
     3841 gtttgattct gtaggcgtag gcacttatcg ttatgtattt ggcagtaata ctcctggcgt
     3901 ttatcgtgtg agtgctttta atgaaaaagg gcgttcttct tatagtaatg aactagaagt
     3961 acaaaatgct gttacagacc tcatccattt acaaaagcca accaatttgg taggcatacg
     4021 tttagctcca agtatatggc aactttcttg gaattatact gcgatagtgg agcgtcgtga
     4081 aaatggattt atcattgaaa agtatgatcc aactcttttg aaatggattg tatgggatac
     4141 agtggataca gatgtcaaaa gaatagttct tgattctgtg gtaggggctg ttgatttaag
     4201 tttgggttct tatcgagtgg ccgcttttga ttctttaggg cgttcagatt attcaaatga
     4261 tattcaagtg aatattcaaa cagagtattc tcctgacata atttttgttc cacctacgga
     4321 tatggtgcca aatgcaagta attctgcgta tcttgaatta ttaattccta ctaatccagt
     4381 gaataaaagt gtggaatttt ctccatatac ggctagcttg tcttatgaag ctctttgggt
     4441 aattgaagac acagaagaat cgcctgtttc tttcagtttt tctaaaaatt ctgtaacaca
     4501 tagcgttgat cctgcgacta atgctctatg tgattcttat gcacgtattc gtttagtatg
     4561 gaccgatata aatggagttg tagattactc cgaatggtca gttccaacgg gttctaagcc
     4621 aggtacacga gggtctgggc taatcgatct taaagaatct ccaaattgcc ctgaattgat
     4681 aaaagtaggc caataaattt tataaacacc atcttttaga tggtgttttt ttatgagaaa
     4741 aaccagggta gcaattacta gagattcttt aacatttaag atgtagcttt ctaagtactt
     4801 ttcctaacaa ttcatttaat atgtctttcg catctgaact gcctaaggat ttttgaatga
     4861 caagtgttaa agtaaagaga aatactccta ctataatgct ataagaaatg agagttagaa
     4921 gacttgtatt tcgaataaat tcattagaga atgagtctaa gaaaagagcc acaacaatca
     4981 tgagtgcaag agaaactacc gcgcgaccta tattcgatag agcttctttc atgccgtttg
     5041 ttccgttatt tttggcccac aaaaaaatca tagcaatcac ttgagcgata gcaccagacg
     5101 ctcctacaat aggaacgctt ttaattccga gtacagggcc caagaataaa taagctggga
     5161 tggtgagggc aaaaatgccc gtgtttaaga gagtgggagt ccacattttt tcggcagcgt
     5221 aaaaacttct aacaagaacg gcttgcaaac aaaggccaag gcttactggt aaataccaac
     5281 gtaatatttc agaaatggct tctgttgttt ctctagtaaa ggctcccctt tcaaataaaa
     5341 ttctaaccgc tggaaatgat aaagcccata cagcaacaac ggcagggatt aatatgcaaa
     5401 acattcttga gatgcttttc catatttttt tgttgagttc atcaatgtgg ttttctttaa
     5461 caagtcgagc catgtctggg taacttgcga caccaacaga aaaaccaaac acagcgacca
     5521 aggtatacat aattcgataa gcgtaattaa ggctagaaat accactagat ccaaaatgcc
     5581 ctccaaagct tcgaatcaca aactctagag caaacatact gcctacacca agagacattg
     5641 ggagcatcat ttttaggtaa cgaataatat cagggtgttt aggattaatg tagaagaagt
     5701 attggatttt gcctcgataa gcccctaaga tttgtaaaac aaaaaatcca acaaaagcac
     5761 ctataggaac accccaagca aatccttcta aaccaaagtc tgaaaataga atgcccccaa
     5821 caataatagc agcattatag ataacgccag ttaaagaggg aattaaaaat tgttttcggg
     5881 tatgttgaac agcaactaaa aaagtgcctg caaaaataaa aagttgcgca ggtaaaatta
     5941 aacgtccaaa atacgtggct cgttcgagaa gttctggtga aatgtctgcg acagtaaata
     6001 attcaatgag ctctcgcatc catataaagg aagggataat taggaataaa agaaaaagac
     6061 caagagtatt taagatgttg ctaaaaaatc gccaagccgc ttcttctttt ttttgaacga
     6121 gataaccagt aaaaataggg ataaaaataa tcgataaaaa accagtgctg acaatatgat
     6181 ttaatatgtc aggaatcata aaggcgagat ctagagcatt tttttctgtg gaaacgccag
     6241 cagctcgcgc taaaagcatt tcacgaaaaa tgcctaaaac acgactagca agcatgctga
     6301 ttgcaataat tatagcggct ttattcataa tcagtaaata taacattcat ataaagggcg
     6361 ccttaaaaaa agatggtgat agccaccaat tagataatca actgtttcgt tgaaacaagg
     6421 ttttcttata taacagcatt ttgagtatca ttaaaccaaa accgttaaaa agaactcaag
     6481 tatgattaag atgtttataa aaaatgaaaa aaacgaacgt aaaaacattt gtaaacaata
     6541 attgattaag gggcggattt atggattatt tttttattta tatagttcta tagatttttt
     6601 atcccattgg agtttgcatg agaattttgt ttattggcgg tacaggcacc atcagcatgg
     6661 cgatctcaaa aaaattattg gctgatgggc acacattata tctaataaat cgaggtacac
     6721 gcaatgattt attagaagga gatattcatt ctattgtggt ttctgatatt aataatgaag
     6781 ccgaagtgaa agaaaaaata aaagacttga cgtttgatgt ggtggctgat tttattgctt
     6841 ttgtgcctgc tcaagtagag cgagattatc gtttatttaa aaataaaaca aagcaatata
     6901 tttttatcag ctctgcttcg gcataccaaa agcctttatc tgattatcgc attaatgaag
     6961 gcacaccttt atcaaaccct tattgggaat attccagaaa caaaatagcc tgtgaaacct
     7021 ttttaatgga ttgttatcga aatgaagggt ttcctgtaac tattgttcgc ccaagccata
     7081 cgtatgatga acgttctatc cctttgggtg ttcacggaaa aaacggcagt tatcaggtga
     7141 tcgacagaat gcttcaagga aagcctgtca ttatccatgg tgatggctct tctctttgga
     7201 ctttaacaca taatagtgat tttgcaaaag gatttattgg attacttgga aacattcatg
     7261 ccattggaga atcagtacaa attacaagtg atgaaacact gacatggaat cagatttacc
     7321 aaaccattgc ccaaaaatta ggagtaaaat taaatgcata ttatgtttct tcttcttttt
     7381 tggcggcatg tagtgatatg gatttagaag gaagtttgat tggagataaa gctaattctg
     7441 ttgtttttga taatactaaa ttaaaacgat tggttcctgg cttaactatg acaaaacgtt
     7501 ttgatcaagg aatagaagag actctccaat atgttttatc tcataaagaa tgtcaaaaga
     7561 aagatcctga atttgatgct tggtgtgacc ggattattga ttctttagaa aagatcaaaa
     7621 ataaaggtga atgagactta gcactgcaat ggttttttct cgttttttta tgaataacac
     7681 cagtattgac ggaaatggtt ttttcactaa agctcatgtt actcttgttg acggaaatgg
     7741 ttttttcatt aaaactcaat aatactatgg ttgacggaaa tggttttgct ttctcgctcc
     7801 gttgaattct attcaagagt cgctctaaag taaaacccat tttcgtctag taattcacta
     7861 ttatgattta attaactggt gttatttttt tctactcgaa ccttcactat ttgattatcc
     7921 aatcaaataa cgttcggttc ttcatgaaaa aaactaggct gggttagatg agcgtttctt
     7981 tttcgtgcta ttgcatttca aaacgagttc gtcggcaaca tccatttccg tctgctattt
     8041 tgatagtagt tgttatatga atagtattgt tttttcattt gaatctgcac tatttgtttt
     8101 ccaaaaacac gaacacttag gagtttactc ctttacgtga gacttagagc tttagctctt
     8161 tagtcggaac catgcatagc tgcgcttagt aagagtgata cccttgcggg acaaattacg
     8221 cttgtctctg catgaaaaaa gcatggtttt ttagatgagc ttttcattgt ctcgacatga
     8281 aaaaactcgg ggcatagctg cacttagtaa ggatgagagt gatccccttg tggtaatgaa
     8341 ttttagaggc ttctttaaga agtctgtctt tttttgtaac tttgcgttat ggcattactt
     8401 tcagttcaga atttaaaatt gacttttggc ggccctcccc ttttagatgg tgtatctttt
     8461 gacgttgaac ctatggagcg aatatgtctt attgggcgaa atggagaagg gaaaagtact
     8521 ctttttaaaa tcttgtctca agagatggct tcagattctg gagaagtgat ttcgaaaaca
     8581 gggcttcgaa tggctcgttt gattcaagaa attcctgctc atatggacgg aacagtgcgt
     8641 tcaattgttt ttggaaatga ggagcacgaa gggtattctg caaaagaagc gattcttgga
     8701 aaaacaggaa ttgatccaga gctcccctat gattctcttt caggtggcca gaaacgtcgt
     8761 gttttatttg ccaaggctct tgcccttgaa cccgatattc tcttgttaga tgagcctaca
     8821 aatcatttag acattccttc tattcaatgg cttgaaggaa ttttaagccg ttacaacggt
     8881 gctattttat ttattagcca tgaccgcgct tttgttcgtc gcttagccac gcgtattttg
     8941 gaattagatc gaggacaaat tcgttcgtgg aattgcactt atgaccaatt tgtgaattac
     9001 caaatgggac aaattgaaga tgaagaacgt gctaataaat tattcgataa aaggcttgct
     9061 gaagaagaag tatggattcg aaaaggaatt aaggcgagac gtacaagaaa tgaaggccga
     9121 gttcgtgcat taattcgaat gcgtgaagaa agaaaagaaa ggcgaagccg aacgggtcaa
     9181 gtttcaatgc agattactga agcggatcgt tctggaagac tagttgctcg tttagataat
     9241 gtgagttatt cttatgaaga aaatccatta atacaaccct tttctacaga aatctctaga
     9301 ggtgatcgaa ttggaattgt tggtgaaaat ggaagcggaa aaaccacttt attgcgttta
     9361 atgttaggag aattaactcc gattacaggt gatgttcgtc ttggaaccaa tttggaaata
     9421 gcctattttg atcaaatgcg aaatcgattg cgcgaagata aatcgcttgt agaaaatatt
     9481 ggagatgggc aggaatatat tgagctccaa gggaaaaaga aacatgtttt aagttatttg
     9541 caggattttc ttttttctgc ggatcgcgct cgagggccaa ttagcgctct atctggagga
     9601 gaaaaaaaca ggttattatt agcgagtctt ttttctagac cttcgaatgt attagtctta
     9661 gatgagccca ccaatgatct agatatggaa acgctcgatt tattggctga tttattagga
     9721 gaatacaaag gaactgttct tgtggtaagt catgatcgcg cgttcttgga ttctattgcc
     9781 accactatta ttggcattga agaaaatgga atgatttatg aatgtgtggg tggttattca
     9841 gactatgaaa aatcgaaaaa acgccgagag actgaaataa cagaaaatgt ttctttaaaa
     9901 caatcaaaag aaatttcaga aaatcgaatt tctaccacaa agaaaaaacg tagctataaa
     9961 gaagaaatgg aatataatgc gcttccagat aaaattgaac aattagaagc tgaaattgag
    10021 aagttacaaa tagagctgac agacgcctct gtttatacta atccagaaaa attgataaaa
    10081 atacaaacgg aattagcaga aaaagaaagt cttttattga aattctatga acgatatgaa
    10141 gagcttgact cgataggaag ttaatagcaa aactatatca attgcattgc tgcttcatcg
    10201 caaattaaag caagcatacg ttcatgagta caaacttctg gagacacttt agataaggta
    10261 ttggttgttg aaaaagtaat acaaccgcat tcattaccca cacatcgact ttttaattcg
    10321 cattctttac aagtttcttt atcacttttt aaaaaggatt ttatttcttg agtcttggat
    10381 tcatcaatcc ctgtataaac agttccaatt ttatataaag catcaggttt agaagagaca
    10441 aaacgagtac atggaaacaa cgttccattc gcagaaactg caacaatgcg ttccaggatg
    10501 gagcaagttt ttttcttata agaagtactt tctaattcta agcggatttt atcttgtatt
    10561 gttcctaaat aaagcgggtg tttctttttt ttaagagaga cccaataaga agctattttt
    10621 tcgtactctt tttttagctc gttaaaatga tctgatgtcc attttccatc aaaatcaaca
    10681 gccgttgtga ctttcgtaaa tccttgattc acaatccact cgatagagtc gcttaatccc
    10741 tgaatatggt catgagtgat tacacgtaaa atagtggtat tcagttgacg aatcgttttt
    10801 aagtgaggct caatgaatgc aaaagtaccg tgtttacgaa tagtatatct ttgtttatca
    10861 tgaatttctg caggaccatc taaagacaaa tacaaataga agttttcttt cttaaaaaaa
    10921 tcaagccaaa aagaattaat caaggtccca tttgtgttaa cggcaaattg aagtttcatc
    10981 ttttcgggtt tgtatttttt agcaatgtca acgcctttta agatgagatc ttttttaaga
    11041 agaggttctc cgccaaaaaa agtgatgttt aagaaagagt cacctaattc taaagaacgt
    11101 tgaaaaccat aagctatggc acgctctaag atatttaaat tcattgagca aaaacgttct
    11161 tcttgaagat gattataata acaataggaa cagcgtaaat tacactgttc agtaagattt
    11221 aaaacaagat tcatatttta aaattaaagg atgagagact catcatctaa tgaagcattt
    11281 gttgaagatg aactttcagg tatttcgaca atacctgctt taggggagtc cgttgaagaa
    11341 gaactatcaa ttatttgtac tccaccagct aatggaggtt cttctgagga tgaggaggac
    11401 acggaagagg atgactcaaa gcgataaacc tcaataatag ttccagccaa cggttgaatt
    11461 ggatcaacag gaacaccacc tataggaata ggagttttta ttgaagatga gctttcaggt
    11521 acttctgttg acgaagagct ttcaggcatt attatttcac cacctaatgg aggttcttct
    11581 gaggatgaac tttcaggcat agctggatca cctgataaag gttgttctgt aatatcacaa
    11641 cctgcaatcc ctaaagaggc agctaaacca agtgctgcta aaagcttggt ttttccgtta
    11701 ttaggatagg attttttttt ctgaaagtgt tttattttga tattcataat attaagtccc
    11761 aaacaattat tctatagata aaataatgaa taagaagatt ttttgcaaag agtaaccatg
    11821 taaaagcata aaaccatgta ttttctcacg tgatattaca taaaaagttt ttctataaaa
    11881 aaactcgctt aaaagtaagc gagttgtata ataataaaga tttaagaatt agagcttaat
    11941 tgggcaagct ttggcattcc aaatttcttt tgcatattga ttaatcgttc ggtccgagct
    12001 gaatttgccc attctagcga cgttaagaat ggctttttct gcccatgcct ttttatccaa
    12061 atagagtttt tcaacttctt tttgagtttt gacatagtca gcaaagtcgg cgagcaagag
    12121 ataagaatcg tttgaaagta acttatctac aatatgctta aataaatcag gcctttctgg
    12181 gctaaagaag ccgctttgaa ttaagtctag aactcgacgt aaatcatcgt tggattcata
    12241 aaagtcttta ggtctgtatc cttttgcaag aagctcttga acttgttcga ctgttaaacc
    12301 aaaaatgaaa atattgtctt taccgacttc ttcaagcatt tctacgttag caccatcaag
    12361 agtaccaata gtaagagctc catttaaggc aaatttcata ttgcctgttc cagaagcttc
    12421 tgtacctgca gtagaaatct gttctgataa atcagcagca ggaataattt tttcagcaaa
    12481 cgaaacacgg taattttcta ggaaaataac ggatagttta cctttgcaat caggatcatt
    12541 attaatgatg tttccaacag ctgtggtgag gcgaataatt tgtttagcca tccaatagcc
    12601 aggagctgct ttaccaccaa tcataatggt tctaggaacg atttcttttt catctttaat
    12661 ttgaatgtaa agatgaatgg catgtaaaat atttaataat tgacgtttgt attcatgaat
    12721 acgtttgact tgaacatcaa ataaagtatt cacatcaaga tcaacatttt gtgtttgctt
    12781 taggtaaaga gctaaacgct ttttattttc aaatttaacg tccataaagg ctttttgaaa
    12841 agaagcgtct ttagcaaatg gttctaactt tttaagatcg tctaattgac gaatccaaga
    12901 atcgccaatc ttagaagtaa ctaaagcaga taaggctgtg ttcgctttac gtacccagcg
    12961 tctaggagta acaccatttg ttttattgtt gaacttttct ggccaaagct cataaaagtc
    13021 gaggaacaaa tgtgatttta aaaggttact gtgtaaagca gcgacgccat ttacagcaaa
    13081 ggatccaata atagagagga aagccatgcg aaccattttt tcgctacctt cttctatgat
    13141 ggacatgcgt cctaggcgct tggtatctcc aggccacttc atagaaacaa tgcgaaggaa
    13201 tcttgcattg atttcgtaaa tgatttgcat atgtcttgga agaaggcgtt caaataaaga
    13261 aacaggccat ttttcaagtg cttcaggcat taaggtgtga tttgtatagg caaatgtttt
    13321 tgtcacaata tcccatgctt catcccattc catattttca atatccacta agattctcat
    13381 caattcagca atagaaatag ctggatgagt atcgtttaat tggatgacta ctttttctgg
    13441 gaataaagcc caatcattgt tgtgtgtttt cttaaaacga ttcacaatat cttgaagaga
    13501 agcagagcat aagaagtatt gttgttttaa gcgaagctct tttccgttca ttgaggagtc
    13561 gttagggtaa agcactttgg atatggtttc tgataattcc atatcgtgaa cagcggcaag
    13621 ataatcgcca ttgttaaaat aggctaaacc aaaattatcc gtgcttttag cactccataa
    13681 gcgaagattg ttcacagtat tgtttttaaa gccagggata ggggtgtcgt aaggaagagc
    13741 taaaacgtaa tccttcgttt cccagcgatt attcagtttg cctttttcat caacccagct
    13801 gacaacatag ccgtagaaag gaatacgtag tgcgtttgca gggcgagcga tttcccatgg
    13861 gttaggtagg cgtagccagt tatcaggatg ttcttcttgt tgaccatctt gaattttttg
    13921 atggaacata ccatactcgt atcgaatacc catacccgtt gctgggagtt caagtgtggc
    13981 catagaatct aaaaagcaag ctgctaaacg accaagacca ccattgccta agccagcatc
    14041 cacttcttgt tcgcgtagtt cttcaagatc catgctgagt tcatgcaagg cttcgtttac
    14101 ggcaccttct acatcaagat tgagaacaga attaccaagt gttctaccaa ttaaaaattc
    14161 caaagagaga tagtaaactc ttttgacgtc attattgtaa taggtttctt gtgtttgaat
    14221 ccaacgatca atcaatcggt cacgaacagc ataagcgaca gcgagaaact tttcgtgctc
    14281 agccactgtt ttttcattac gggctaatgt gtgatgaatg tgatctgtaa aggctttttt
    14341 aaaggcttct gcagaagtat ctaaaacgga ggatgcagaa gaatcatttt ttgaagtgac
    14401 tttagccata ttatcctttg aatgtgaaag gtattttcat aaaagaattt agtttttttt
    14461 tgtgtattcg gaaggacttt ttttgctttg aatgtttcaa aatcattaca tcttattttt
    14521 taaacaccct taaaatgatt aatcaaacaa tagactgtga tatattgcta caaatggctt
    14581 ccagaatggg ttgaaagtgc atatgctaaa tatcacattc tcgattttgt tcaatatcat
    14641 gatttaatat cattctaaac ggattatatt atctaaagca attttattaa gggcacctta
    14701 tgaaagtcgt attattaggc tcaagtggtt tagttggagg gttttgtcga gatatacttg
    14761 ctttggaacc taaggtaact catgtgcatt gcttagttcg atcaatagat tcgtctttta
    14821 aggaaagtga taaagttcac tatagtgtgg ttgactttac aaacttaacg ccatacaaat
    14881 ctttttttga agcagatgct gtgatatgtt gtttaggaac aacaataaaa aaagcgggaa
    14941 atcgtaaagc ttttgaagcg gtggatttac aaatgcctct cgctgcagga gctcttgcca
    15001 aagcctctgg cgtaaaacat tttttagcta ttagtgcaat gggtgccaat cctcactctt
    15061 ggattcatta taaccgaaca aagggcttaa tggaagaggg attatcaatg atgggatttg
    15121 aatctttgac tttagttcgt ccttctctac ttttaggaga tcgtgtagaa aaaagaagag
    15181 cggaagattt gtttaagaaa tttttatcaa aaagattgta ctcacttcct gcgtttttta
    15241 ggcctgttca tgcacaatca gttgcaagcg ccatgatact ttctttgttg caaccaccag
    15301 agggaaagcg gattgtttac aatcgaaaaa taacagaatt agaacttcta aattttagaa
    15361 gaactcctta aataacgata caagcatttt aaagtaatta tcgggcattt gattaaagcc
    15421 aaatagctga aattactttt cacaaaagtt ttgatgacct tataaaattc aatttgatct
    15481 tctctttttt gacctaataa aaattttctg aattttaaaa gtaattgatg ctttttacca
    15541 aacacaatag agcatcgttc ttcataattt tttaagaagt ttttagataa atcatctttt
    15601 attaagccgt tatgaatgac ttctgctcct tcttttgcgg cacgcatagc ggcagcaata
    15661 ccaccaccag taagtggatt agtatgatga gcagcatctc caactagaat taaacggtca
    15721 gtaacatact cttttaaatt accagaaaca gaaattaatc caccagtaat tttattgact
    15781 ttagcctctg gaaaaagttt ataaagccac tgtaaagtaa tatcttgaat agaaagatct
    15841 tttgattttg gaattaaaaa accagcaccg aaattagtga cattactctt tacttttgga
    15901 aaactccaaa tgtaaccgtc attaataaaa tcatgccctt gccaaaaagt taaatagcga
    15961 gggtcagtaa gaataccttc tacttcaata tcaacacctg tacaagtaga gttcacattg
    16021 tgaagacatt ttaatccggc catgcgcccc actaaagact ccattccgtc ggcgccgaca
    16081 atcattcgcc cttttatttt ttctgtacct tgtggtgtta caatagaaac ttctcggaac
    16141 tttcctataa cagcgctaac agattcagca cgagcatttg tcactaattc aacgccatct
    16201 tgttcagcaa gctttgcaag acaaggatcg aatttttctc ggttgagcat aataccagta
    16261 tcttttttta tggattcaac cgtggtcccg ccaggaccat agacgaaaag cccatcaata
    16321 atggtttcaa tgcaatcctc atcgattgga ccataagatt cgatatcttc taaagtagtg
    16381 cttgcttctc cgcatcgaac gggaaatccg atttgttctc ttttctcaat taataaaaca
    16441 ctatggccat attgagataa acggcgtgct ataacagagc cacctgggcc tgcgccaata
    16501 acgataacat catattcttt atttttcatc aggcttttct atccaattaa gtacaccagc
    16561 aggacaagct cgaacgcata taccgcaacg attgcattta tcatgattga tttgcaaatc
    16621 taagccgaac atatcaagag ccatcgtagg acaaacacca acgcatcctg cgcactctag
    16681 gcaatttttt ctgtcatgag ataatttttt cacttttaaa aaatacaaat aatctcaaat
    16741 gagcgtttga aaaatatgtg gaaaatccaa gaatttatta gagacttcta aatcaggcgg
    16801 gaaatgctta tataatagat acaaagaagc cgttataata gataactgct tctttgatta
    16861 atggaattca agtgagtaat aaaagaattt attgctttat ttttcttctt tctttttcat
    16921 ttctttttta cattgataca ttaatttatc tgctgtgctt agcatttcat ccatgtcttt
    16981 atataaatcc actgaatggc taattccata agcaaaagat atattctgat caatgatggt
    17041 aacggctttt actgcgtctt gcattcgttc tgcacaaata tctgcaccac gcttatctgt
    17101 atcagggcat aaaaccataa actcatcacc acccatacgg aatagcatat ccgattctcg
    17161 aagcagtttt ttaatagcgg ccacaacgga gcgcaaaagt aaatcacctg cttgatgtcc
    17221 atatttatcg tttactcttt ttaatccatt caaatcaaag taaatcaatg aaaaaggagt
    17281 cccataacgt ttacttcttg caaaccattc tttaagagtg tacatggcat gacgtctgtt
    17341 ataacactgg gttagatcat ctgttaaaga aatatcacgt aagaagttta atgttcttgc
    17401 taacctaata tgaacattga ctctggcgag gagtatttct ggcacagagg ctttacttac
    17461 aaagtcagat gcaccagcat gaaatcctct tgtaatgctt tcaaaatcct ctcgtccagt
    17521 taggaaaata attggcaggt catctagtgc atattgctgt ctaatgcgta aacagacttc
    17581 ataaccgttt aatttaggca tattgatatc taacagcaca agatctactc gctctgtttc
    17641 taaaaactga agagcatctt caccagaaga acaaaatgcc actttatagc ctacctgagt
    17701 aaataattct tcggtttttt gtaagatggc tggattgtca tctacaatta ggattttttc
    17761 ttcaatcata aaagcctgcc ttttaacgat atgaatttta agaagatgca aatgatctta
    17821 taaagggaga gataagattt taaaatactc tttttttgaa tgaaaaagat tttttagcaa
    17881 aaagagatat ttcatctttt cataaaaaaa cgtttttatc ccatttcact caattttttt
    17941 taagacctta ggtaaagtgg gtcgcttttt tttgatgata aaaagatgaa attagcgaaa
    18001 attcttaata atcgcggttt tataagataa gcaagaaaaa ataatacttg aaaaagggaa
    18061 ttataatagt tcaacaataa tagcctaaat aaactttaat ttagtcgcag ttggaataat
    18121 acctctttgg tggcagaggc taaagaacag ttctagagat tcttttcttt cagaagtaaa
    18181 agaatagtct agagaatcat aataagagaa aatggttcgg gctggtaatt gaactggata
    18241 attctttaac cacgtttcaa tagctgtttt agggcttttt ctaaaagagt taacgttttc
    18301 ttcagtggca gttaagaata gatccaaaag atcttttttc tctttcacaa gagcattttt
    18361 atgaataatc caagcaccaa aaacaaaagg aaggtgttgc cattcttccc ataaggatgc
    18421 gagatcgtag ctataggaaa agtggttttc atatgtttca agaagtgctt gatcgccaat
    18481 caggaggcgg gctacgtctt ctttagggtc ataggctcta ttgcgtatat atgtaggagt
    18541 aatatgaaaa cgaagtgaaa gtaatatgtc taatagagct attgaagtat cgctttgatt
    18601 tgttaaatgg atgttttttt gattgagttc taataaaggt ctttggctaa atagttttac
    18661 ggattggact tcaaaacggc atgaggtaca aagttttgga gataaaagaa acagttcagg
    18721 attttttgca aaagtgatag aagaagctgg ggctaagtct ataatatttt tgttcaaagc
    18781 ttcattatga gctcgaggag tgccatcaat gaataaaaag ttcggaaaat tttcattttt
    18841 cgtaaaaaaa ttgtaaaaga agggggcaca gaccaaaaaa gggatacgac cgatattgag
    18901 attcataaaa aaaaaggtaa ctttttacca atgagttaca atatcgtttt taacactttt
    18961 caagataatc gaagaacatg ccaaaatttc atgtaaagaa aacgtccctt ggggatgatc
    19021 aaaccagtct tatttttaaa gggaaagtaa tagagggtcc aattaaccga gggatgattc
    19081 tcgaaattcc tattacagaa gcagcggtga ttagtgtcaa ggtatatgac attgttcact
    19141 tcgaaaagca aaaagatgaa atgaagaaag tcggtttagt cgtagatttt tatggcgcac
    19201 cagatgattt agatgtcatt atgggcttaa atattgttga tgaagaacta aacgtagtag
    19261 aagcccctgt tggttcagaa gtttgatctt caacctagag ggctggttca tttttatttt
    19321 agttgttttt gaataattaa tttgtctgta ttttagattt caataagaaa gccggctcgg
    19381 atgtaaaagt agtcgtcttt cttttttagt ttctgatagc cagccatgag tattagagaa
    19441 gagtaatctc catttttaat atctatacca aggcctgcgc tccatacgaa ttgatgatcg
    19501 cttccaactg cataaccata atcggcaatg attagaggga gaacttgctt gcctattggg
    19561 agtcttatcc atacactccc tgtcacaggg ataaaaatag ttttatcaat ttcttgatag
    19621 taaccaccag caccaataaa aatcatttga tcaataggaa gccagagatg gcctcctaag
    19681 ctgatattgg ctcggtctgg ttttggggca gtcgttaatg ctccaacgga aagatccatg
    19741 gttaaaagag cattagcgtt agggacagca acagaaagag aaagaataaa gcagataagg
    19801 aaagaaatgc ccttgatgga aacagtattg gcttgatcat cagtacgtgc ttcacgttgc
    19861 catccgccac ctttttcgcg tagaaatgat aagaagaaac ctttaagtat tgctgtattc
    19921 atagataaaa aataaaatgc gcttggaatt aaacgaagtg ctcctattaa aattaataaa
    19981 gtgaaaaaga caaaggagcc aatataaata ggctttttag ttaatattaa gactaagtta
    20041 gagagaatta aaaaaatgcc taaatgagga gtaaaccaac gcagtagttt atgggaaaag
    20101 aataaataag ctaacagagg gcgaaatggg tttaataatg ctaaataaga aaataagaag
    20161 ttaaaatttg ctcttccaat acggactttt cgacggaact caccaatgga ttctttagag
    20221 gtttgttctg taccaattgc tgtggataca aaagtcgaga aaaatccttt ttgaagtact
    20281 tttacagtaa taaagaaatc atccattacg ctcttgtttg taggaagagg agtgtatagt
    20341 tcatttcgaa tagcgtagag agcaccgttg cctccaatta atcgatctaa aaccccttca
    20401 aaacgtttta tttcagattc taaatcccaa taagagcttt ccccttgccc gagaatacta
    20461 ccactataat cggataaaat taaatgacca caggagcaac caacttttgg atcttcaaaa
    20521 ggagctacaa ttttgcgaac cacatttgga aaaaacaatg tattggcgtc acaaaataga
    20581 aggatttcac cttgggcttc tttttgcaaa cgatttaaca ttgctgcttt gcctgcattt
    20641 ttttcagctt gaattaggcg aatcccttgc tctttgtatt ttaagatgat ttctgcagtt
    20701 cggtcaattg atccatcatc accaataaga atttcaagtt tttccttggg gtaatctagt
    20761 tcaagtaggt tttggatctt tttttcaata atgccctctt cattgaaagc agaaataaga
    20821 atagaaacct taggctggaa ttttccagaa aaagacttgt gtttacgctt aaaaaactca
    20881 cttacaaaag gaagagttat aggaaaaaca agataacagt gaataattaa acataaagaa
    20941 atccaaaata cgatttcgct gtaaaagagg agagacgtca ttcttgtatc cactcctttt
    21001 ctttattgag aaattgttgt aacaaaaagc gcatttcact gctttgaagc gattgtgtta
    21061 gtgtcaaaat agttaatcct tttggagcaa ttaatacaaa agaaggaaca ccatttaatt
    21121 tccaaatatc aaaaaaacaa cgttccaccg ttttgattct tccatcacac acaatttctt
    21181 cttctgaatt tacatccaat tttactggat gaaactttgt attcaaaata tttacgataa
    21241 atggatcttt aaaaacattt ttatccatcc ctcgacaaga agtacaccag tttgcagata
    21301 aataagtaaa aaccatcttt ttatcaacaa cggcattttt caacgcttga tcatagggct
    21361 cccattttac tccgactgga ttttctttcg agaaagcagt tagcgttgct agtaataaaa
    21421 aacacaaaaa aagacggcct ttaaaaagga acttgcatac agaattttga gaaaccaaca
    21481 ttgatctcat tttcttgttt ccgggaggga gtcaacaata ctgactactt tttgttgaaa
    21541 taaaagccat aattttgggt ctgttttgat tctctcggat tcttgattaa atgattcggt
    21601 cgtgtagtta tattttttta aaatctcagt tcttttgatt cgtgcgttag gattaacgtc
    21661 tccaagttct agtgagacga ttcttaattc tatatacgca ttcacaaatt cttcagagat
    21721 ggtaggagaa agtgattcac agcctagcaa ataaatgcat atgcataaaa gagacagcca
    21781 tcgccaccga tgtgtttttt taattttctt cgtcatttaa gagagcgttt tcaaataagc
    21841 catctatgta ttctttttta ttaaatggaa caagttggtg gatttgttct cccataccta
    21901 tccagcgaat gggaatttgt aacgcacttg aaatgcttaa taccgaacca cctttcgctg
    21961 taccatctag cttagtgatg acaaggccag tgagtggaaa gctttgatga aaaactttgg
    22021 tttggttaat tgtattttgc ccagtatttc catcgatgac aagccagaga tcatgggggt
    22081 aagacggatc cagtttttta attactcgaa ccactttgcg aagctcttcc attaaatgat
    22141 ctttattatg taatcgacca gcagtatcaa ttagaacaac atcacagcct cttgcttttg
    22201 ccgcttcaca agcgtcgtaa gcaacagctg ctgggtcaga gccttcttga tgtttaacaa
    22261 attcagcttg agaacgttcg gcccaagttt ctatttgttc aatcgctgct gctctaaaag
    22321 tatctccagc ggcaattaaa acctttttcc cttcagatgc aaaacgagac gctagtttac
    22381 caatggttgt tgttttacct gcgccattga ctccaatcac taggatcaca tgaggattgc
    22441 ccttgagttc aaaaggagga ggatcagata gtaaacgctc cgcttcggct cgcataatgt
    22501 taagcacttc atcagtagta agtgatttac caagagcgtt ttctctgaga gcatctgtta
    22561 aaagaaaagc tgcttctaca ccaacatcag ctttaattaa atgttcttct aaattttcaa
    22621 gcatttcatc ggtgattttc cctgcaccaa caatgccttt cagttcgctg agaagagcgt
    22681 ttcttgtttt ggtgagtcca ccttttataa cagataaaag acccatatta taaaatactc
    22741 ttaacagcgt cgactaagcc ataagcagcg gcttcttcag cagacataaa gttatcgcgt
    22801 tcagtatcac gatcaatatc ttttaaattt ttaccagaat gtttggcaag aatttggcct
    22861 gtaacactac gaatgcgaag catttcttca gcttgaattt gaatatcgct tgctggcccc
    22921 accatttcac cgtggattaa aggttggtga atcataatgc gagcattagg ccaagcaaag
    22981 cgttttttct ttgcaccagc agttagtaaa actgctccca tagaagcggc ctgaccgcaa
    23041 cagactgtta cgacgtcgct tttaatggct tgcatacaat cataaatggc aagaccactg
    23101 gaaatgactc caccagggct attaatgaaa agaataatgt cttttgtttc taatgaatct
    23161 aaatacaaaa gacgttttac cgttttttct gcggattcat cgtcaatggc tccccaaagg
    23221 aataaacgac gttctttagc taaatactct tcagctgtct tgagaaaatc aggggtttta
    23281 gtttcgtttt ctttctcaca atctttcttt tccattaaaa aactccgtag gggaaaatta
    23341 tacgcttcta atttagaaaa aagtacgtat ttttgggaaa tgaacaatgc aggaagtcct
    23401 ctcatagcag gcattatttc gcagacaaat ttagttcagg ctgaatcgga gcttggagga
    23461 gttattcatt ctcgtctttt gctttgccct gttttagaaa ttcgctatga tctttttcaa
    23521 aatgtaaaag actggccgct tttggcggga agagtgcgtg cattacatcc gactgcaaaa
    23581 attattggaa caatacgatt aaaacgagat ggtggggcac ttgaggattc tattgtaaaa
    23641 gaaaggcttt ggctttggga atcgatttta agtgcagacg tagttccatt atggatagat
    23701 ttagaactat ttgcattagg gccagaagct cttttactta aagaaatggc aagtgctaag
    23761 ggatctcgtc tttttatttc tatgcatgat tttttaaaaa tccctaccac tccagaacta
    23821 gaattactcg caaaacgggc gatagatttt ggtgcttgtg gtgtgaaggt ggctgcaatg
    23881 agccatagtc caggcgatgc acgccctctt tatgattttt taagacgaat agaatcacag
    23941 tttgaattac gggcggcttt cgcgatgggc gtctctggat ctgttagccg agtttggtcc
    24001 cttgcgatgg gagcgaattt aacttacggt agcatcagtg atgttttggt tcctggcttg
    24061 ttatcagcca aaaaaatgac tcaagcaata caatttttac gagaatgtaa aacagaagag
    24121 caaatgagtc tctttttgga agagataagg gaatcgtaaa ccatcaggtt tatctttaat
    24181 aacaattgaa tattttgaaa aaaacgcatg tctatgcgta tatttaaatt attgcgttaa
    24241 ttatttgggg tactttttag cttggtacat tggataaaac
//
LOCUS       DAOOBB010000007        21532 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_3221_length_21532_cov_4.528426, whole genome shotgun sequence.
ACCESSION   DAOOBB010000007 DAOOBB010000000
VERSION     DAOOBB010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 21532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21532
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3221_length_21532_cov_4.528426"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PLT31_00780"
     CDS             <1..440
                     /locus_tag="PLT31_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93698.1"
                     /translation="KAWVKSLFALFIGALLLSGFAVWIEVSLLSWACLLILLFADALT
                     VGWYTGVHLKEGKPKAGSIIQASLKQLPVIAIGAFVALVISLFVENLWSLEAVLSQSL
                     FWMAYLGVFYLIFSPYYSFMSLDQLRSQKRQVKIPNSGASKRS"
     gene            complement(501..1943)
                     /locus_tag="PLT31_00785"
     CDS             complement(501..1943)
                     /locus_tag="PLT31_00785"
                     /inference="COORDINATES: protein motif:HMM:NF024484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HPW93699.1"
                     /translation="MKNIIVSLLCFLFIAMGNAYAGRASFPLDSTFDYNAPSTVSVDY
                     KEPLPVLIPFGEILVQNVFVWAWDRYHLGVNYAKISPEVWKRNIKNGWTWDNNHFGIN
                     FFGHPYQGVFYHAAARASGYSFYQSVLFDMFGSFSWEFFCENEYPSLNDFIMTSYGGA
                     FYGEILYRLSERLLVKPKPNWIDHAGAFILNPYTYLHRRITGTRPNHPHYFPIELSVF
                     TGAGFRFGSDYRYDKSPADRLDDKWEELFGFAGLNLVYGSPNRVLKKPFEYFTIQATQ
                     EMGQRERLFNLEVLAKLKNINKVSGKDWFDFATYTSYDVFYGDLVEMSAFSLGLGSDF
                     NVWLRPSLSFRLTSIPSFVILGSTDFNYDDLLKEFYPDYEPTRTYQLNYGVHYKAAFD
                     ISAKYGRISNHTNIFLLKTMPSSAPHYGATGWDIVGFNISNIDFFLAKHINLGLNLKF
                     YFKIAAYEGEYFEPMSRTMESFGLYVKYLF"
     assembly_gap    2109..2208
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2331..2780)
                     /locus_tag="PLT31_00790"
     CDS             complement(2331..2780)
                     /locus_tag="PLT31_00790"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HPW93700.1"
                     /translation="MAFNEEEEMQLAWIQTHPMIDKDLEQKSTKRLTRTKRTNSKKIK
                     KFLQDNPFGEPEQEIDLHGCEGLEAVEKVDSLIHAMIPAGYKVLRIIHGGGNLKYGNV
                     KRFVDHHIKTRLKHLVAYYGLERNNPGSSLLKIKSDIEKAPLFKKPS"
     gene            complement(2770..3606)
                     /locus_tag="PLT31_00795"
     CDS             complement(2770..3606)
                     /locus_tag="PLT31_00795"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl acyltransferase"
                     /protein_id="HPW93701.1"
                     /translation="MRKQLYYLFLFHWIRWPNFFFDIVFAFIYPLYKFTHRNRAWGRV
                     ERLLKETSFDTKTNPRAVFYSLYKNAILSYRSLLGHKKTLSNIFYENEFLIQDLVKKN
                     VPIVAISVHQGPFELLHRSLCRYHRLIYLLTNPFSDTSLTQALKKIRSHSHLKEYSPD
                     DTHLVLKKLIREKGILAMLVDQAKNSKGVLTRLWDKNFNLYLRLPIKANQMGAAIITF
                     RTFSVKNQIIVRFEKIYPPKSEANTITQGISNEIESWIEEHPEQWTWNYHRNFRVDDY
                     GV"
     gene            complement(3613..4944)
                     /locus_tag="PLT31_00800"
     CDS             complement(3613..4944)
                     /locus_tag="PLT31_00800"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HPW93702.1"
                     /translation="MDLRKTLSELGQEHLLDNIDSLSEADRLQLEKEIASQDFNLLNH
                     LFKEHSSTPVISTEDEILPLTFDLAENNIKQAMWEETGFLLLEKGQVAAFLVAGGQGS
                     RLGFEGPKGAYNIGLPSGKSLFQIQAERISNLSSRSGHPIPWCIMTSPLNHEDTIAHF
                     EENHYFGLPRDYIRFFSQGTMCALDPNGKAIKSSPEHLALVPDGNGGCFRALAQSGTL
                     AWLIEKGVRFVFLYSVDNVLVRIADPAFVGALASDGRALSSSKVVRKKHPQEKVGVFA
                     VKNKKPSVIEYSDLPDALRLETYPDGTPLFDGANIAIHLFRIEALKKLQSTPLPWHSA
                     KKTVFGVANAWKFEQFLFDAFPLLGSMTLYGVSREDEFAPVKNADGDDSPKSARLMLG
                     MLHKYWLQQAGVTVESRKLYEISPTISYGGESLSQAVFDRELGKNILAFDP"
     gene            complement(4948..6096)
                     /gene="mraY"
                     /locus_tag="PLT31_00805"
     CDS             complement(4948..6096)
                     /gene="mraY"
                     /locus_tag="PLT31_00805"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPW93703.1"
                     /translation="MLFQWLYHTTGVEMFDGRLFRAGFAALLSIILVLTFMPKYIRLL
                     QGLDATSDLDKDGKSKSPPIMGGLLLVLVVTLVSLFTCKMNGYTISTLVILVLFSSVG
                     AIDDIAKVRAKRLIVQGKLKAADYMDKADGLSSTIRLALYFLFSFVVAIFCYKFIPDL
                     RGNLTVPFCPISFFQIQLPNWAFIGFMTFVIAATANGTNFTDGLDSLVSVPILTSMVF
                     VGLVAYVSGNFIFSSYLHVPYLPGCDELFILAMSIAGSLLAYLWFNSPPAEIYMGDAG
                     SVGFGATIGIMFILVQAGLFLPIIGIIIIAEAFSVIFQISWFKFTKKTTGTGKRLFLC
                     APLHHHYQKKWSDKFSSKSLLNSKIAWRMHLVSIFALIVGMVIFFGIR"
     gene            complement(6366..8255)
                     /gene="htpG"
                     /locus_tag="PLT31_00810"
     CDS             complement(6366..8255)
                     /gene="htpG"
                     /locus_tag="PLT31_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HPW93704.1"
                     /translation="MKTEKMEFQTEVRDLLNLMIHSLYSNKEIFLRELVSNAADALDK
                     RRFLSVSNAALLPVGTTLKIDIAVNTEGKIITIEDNGIGMNREDLISNLGTIARSGTK
                     NFIKSLKEKDQSNVDLIGQFGVGFYSVFMVARKVEVLTMKAGETQGFLWASEGTGDFE
                     ITEAPKQDVGTKITIYLKDDEESLEYANEWQIKSVIQKYSEFISYGIFFHPEPTKNDK
                     GEIEVHEPKRLNEKPALWRQSEKEVTEEQYKEFYNVVAHEGTEPAAWNHSHAEGSMEF
                     WSLVYIPSKAPFNIWNNERAHGLKLYVKKVFIMDDCKELLPPWLRFVRGVVDSEDLPL
                     NVSREILQSNKIITNIRKHVIKKVLDSLQQMADKDVEKYTEWWRELGAVLKEGFYMNW
                     EHLDELKKLLRFRSTKTEGNSLISLTDYVSRMPETQKEIYYLIGDRKEGIDSNPLLEA
                     FRSKGYEVLLMSDPIDEFMMSSLTEFSEKKFHDISRGDVDFEKTEDEKKAEESSKSEF
                     KGLCEALQKTLDSDIKEVRISSRLKESPCCLVTSEDAMSSRMERMMEAMGQGKMEKSK
                     RILEINPTHPICEMLKAKIDKAEDLEDWPKALYGQALLAEGSPLPNPSEYVSAINKLL
                     ARLAK"
     gene            8432..8920
                     /locus_tag="PLT31_00815"
     CDS             8432..8920
                     /locus_tag="PLT31_00815"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HPW93705.1"
                     /translation="MLISAIVAISENNVIGRDGHLPWRLPADLKYFKSVTTGHHIILG
                     RKNYADIGRPLPDRVNVVLTRNNAFSAPGCVVAYSLEEALQIAKDAGETECFIVGGAA
                     IYKEALPFCKKLYITRVLASIEGDVQMPSLGEGWVLRSEEKRDADEKNAYPMVFQVFE
                     RE"
     gene            complement(9022..9906)
                     /locus_tag="PLT31_00820"
     CDS             complement(9022..9906)
                     /locus_tag="PLT31_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HPW93706.1"
                     /translation="MASIFSMTGYGKVETAFNNHTIVIELRSVNNRYLDITTKIPKNL
                     SHIENDLRNQIKNKVARGSVFLSILMDSGETGNIPTSYNEAAVKAFIDIANDIKDKFN
                     IEGNVHLEHLLSLPDLIQYSDSSQDQEELEKHIALNLDLAIDQMIEMRKKEGENLVTD
                     LKARISTIENILNEIERLEPTRICDWKEKFEERLKGLLQNLELDPIRLIQEASIVADR
                     LDITEEIIRFRSHNQLFLNTLKTGGSQGKKLGFILQEMGREANTLGTKCQCAQIAACA
                     IQLKDAVETIREQVMNIE"
     gene            complement(9921..11702)
                     /locus_tag="PLT31_00825"
     CDS             complement(9921..11702)
                     /locus_tag="PLT31_00825"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HPW93707.1"
                     /translation="MSEFLHKDSLGPVVPQDMKIELGSEGFLLEGGGRLKELSIRYET
                     YGELNKEADNVIWICSPLTADAHVAGFYKPDDKKVGWWDALIGKGKAIDTDHFFVVCS
                     NILGGCKGTTGPASINPETLKPYGASFPNITIGDMVQAQKKLADGLGIKKIFCVIGGS
                     MGGFQAMKWSILYPEEVERCIIIASSPRFSSQALGFEIVGRDAIKQDPNFNGGDYYEG
                     EIPAVGLSNARKIAHLTYLSRVGMEAKFTREACKDPNPKEFLMGFPLESYLAYQGAKF
                     VERFDANSYLHITYATDTFDLETEYGSLEKAFKKTNAKFLNITLSTDWLFPPHESRKI
                     THALVNAEKEVTSLELDTLFGHDGFLLEVGDLGKAISAFISPDPKLNQHSDTQAIPVF
                     HDTEDFDLLETAIPNNSRILDLGCGNGDLIERLKIRKNVQGLGVDKNFNSILSCLRKG
                     VPVIQKDLDEGLKDFGNHSFDFAILNRTLQEVNMPLDLLHEILRVAGKAVITFPNFGH
                     WSVRAALCRFGRMPKSSELPYEWYNTPNIHLFTLRDFIRMCESENLTIEKLYFDNDKL
                     FSKILTSIGRTNLGAERVVALVSKKDK"
     gene            11773..12201
                     /locus_tag="PLT31_00830"
     CDS             11773..12201
                     /locus_tag="PLT31_00830"
                     /inference="COORDINATES: protein motif:HMM:NF017345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HPW93708.1"
                     /translation="MSTSSIVVTPQIIEFPRILDERGNLSFLESERHLSFKIKRCYWI
                     YDVPGGELRGSHAFKNQHEVIIALSGSFDALIHDGVEEKRFSLSRSYYGLYLPPLHWR
                     TLDNFSTNSLALVLSSTSYDEDDYVWDFEEFLKLKKGSSK"
     gene            12198..12614
                     /locus_tag="PLT31_00835"
     CDS             12198..12614
                     /locus_tag="PLT31_00835"
                     /inference="COORDINATES: protein motif:HMM:NF017345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HPW93709.1"
                     /translation="MIKTRLSDCSLITLPRHSERSGSLTAVESDKPIPFDIARVFYLY
                     DIPGGESRGGHAHYECHQLLIAASGAFDVKMDDGTSSKIYRLDRPYYGLHIPPGIWAE
                     EISFSSGSICLVLTSHHFDELDYCRDYTCFLEQKKD"
     gene            12617..13570
                     /locus_tag="PLT31_00840"
     CDS             12617..13570
                     /locus_tag="PLT31_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93710.1"
                     /translation="MGHLITNGIECIQVSAADYSHFLTSETIVYQSVAFNELNASKVS
                     QLRYLLFKRDGKAIAGLIVGEKGNVWLSPFSAPFAGFSFSKSFSLTTLDSIVSCLFDY
                     IKKLEIVLQITMPPLFYKQDLYLKLAYVLQRYSTSSYTDINYALDLKTDDYDSILIST
                     SRRHLNRAKSIPYVLKQVDTLEEKKLAYDVIAQNYKAKGYPLKMSLNDLEKTMTFIHG
                     RYFLLSMDEIHVASAIVYEVTPTIAQLIYWGDLSAYSLKRPMNLLAAELFEIYKKRGF
                     EFLDIGPASELGAPNTGLCSFKESIGCFADLKYHFTLKGNT"
     gene            13572..14678
                     /locus_tag="PLT31_00845"
     CDS             13572..14678
                     /locus_tag="PLT31_00845"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPW93711.1"
                     /translation="MVRFLDLQKLNQFYEPSLSQEAKKIIDSGFFIRGRACSEFESAF
                     ALFCGVPYCIGVGSGLDALTLIFKAYIELGRIKAGDEVIIPANTYIATMLAVSETGLV
                     PVLVEPDEESFNLDPLLLEKALTPKTRAILPVHLYGRSANMNPILSFAKEHQLLVVED
                     AAQAHGAYYGKNRVGSLGDAAAFSFYPSKNLGALGDGGAVTTKDVSLAQVVRTLSNYG
                     AQKKYVNTYKGVNSRLDEIQAAFLSVKLPYLERHNARRRSIAERYLNEIKNPLIRLPQ
                     MGVSEEHVWHAFVLRTDYRDEFIKHLQNRSIETLVYYPSAPHQQEAYKTGLVYESLPI
                     TERLAATVISIPLNPILTDDEVTEIIQGVNDFVC"
     gene            14668..15234
                     /locus_tag="PLT31_00850"
     CDS             14668..15234
                     /locus_tag="PLT31_00850"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPW93712.1"
                     /translation="MFVKIFRHAYAFMRRLLYSFLSEASYEGKPIKGAPLLTQGKGRI
                     HFGSGVRLGWITGKSFWNSYIFCDARTSSSQISFGKETWIGNDFSAISEGPGIFIGER
                     VLIGSHVDIYDTDFHPLSPENRLGGEPKKGAVTIQENVWIGNRVTILKGTTIGAHSVV
                     AAGSIVSGTFPERVLIGGVPARVIRSLE"
     gene            15241..16512
                     /locus_tag="PLT31_00855"
     CDS             15241..16512
                     /locus_tag="PLT31_00855"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HPW93713.1"
                     /translation="MKKMPQVIKQSFLSGMVAFIRIVTSFATNKILAVILGPVSFAMV
                     GQFQNLMMAAQAGGSLSIQNGWVSLTSQYAEDEDRRFSLWRSGLILSGISILFVSIFL
                     LIASWTGAFHFLFPDVSERMLRIALLCAIPGVIGMVLFSIVQSIANGFSAFKLWAFLS
                     ISTYLTQALWVILFVLWNKDLILIVLATQSLVALFISLFIAYKKKIFSRLIKSTSFGY
                     NVWKKYALMGIIPMILTPIVLTAIRSLIGTSIGWHEAGLWQGAFRISDFFNVAFSSVL
                     GVILLPKLSKLKNEKSFFKTLWYALLGVLLVAFILIFSMFFLKEFVIQFILSEEFLPV
                     AKQMHLQWIGDFFRCGSWCLGIGLIAKQSAKAFLFLEILSQSLFFILSFIGISSLGIT
                     APFGAYAIENVLCFIFILLFTIRNHYTWKPL"
     gene            16497..17369
                     /locus_tag="PLT31_00860"
     CDS             16497..17369
                     /locus_tag="PLT31_00860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPW93714.1"
                     /translation="METPLVSVLLASYNHEAYVEAAIRSILSQQGVSFELIVIDDGST
                     DQSPLILESLSKKLGFQFITRPNQGFVSTMNELAKLARGRYFCSFASDDIMPAGRLMI
                     QSTFMEEHPNVPVCFGQTMEMNASGTLELNLDERFLKGVPEVTFEELFLGQKALHGCT
                     EMIRRDLFFEVGGYDSRFFFEDFPLWLRLSYQYGPLPVLENVFCYYRLHGNNMHLNHE
                     KMYSELLRIIDLYSEHPLYKRAVWHCKSNWFSSLTYSNKMKALQMFFKLASPSWMFLR
                     RVPKLFIPRRFLKY"
     gene            17379..18083
                     /locus_tag="PLT31_00865"
     CDS             17379..18083
                     /locus_tag="PLT31_00865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPW93715.1"
                     /translation="MISVCMAVYNGEKYLQEQIESILKQLSPEDELVLSDDASTDASI
                     EIIERIKDSRLILLKNEKRLGPIYNFERALVHCRGDLIFLSDQDDLWLTGKVNACVQK
                     LKEYDLILHDAFVLRENKRQEKTLFELRNVQRGLFKNWWKNSYTGCCMAFKRTILESA
                     MPFPKKIPMHDQWIGICAEKKYRVLFLNSCFIEYRVHDNNATRITGTKKQTFRQRFLW
                     RYYLLKALLSRFSTHS"
     gene            complement(18038..18652)
                     /locus_tag="PLT31_00870"
     CDS             complement(18038..18652)
                     /locus_tag="PLT31_00870"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013352894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HPW93716.1"
                     /translation="MAFTLPTLPFAYDALEPWYDEATVRVHHSKHHQAYTDNLNKALE
                     EGKVETTSVESLLKNLDSVPMALRTRVINHGGGYFNHNFFWENLSPAEKTTPSAFLKA
                     EIEKYWPSFDAFKEAFSAKALAHFGSGWVFLVLTKEKTLEIIDTHDQICPLSLGYEPI
                     MTIDVWEHAYYLKYQNRRAEWISAFWNIVNYECVEKRLKSALSK"
     gene            complement(18695..19168)
                     /locus_tag="PLT31_00875"
     CDS             complement(18695..19168)
                     /locus_tag="PLT31_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93717.1"
                     /translation="MTQENQKDESLASIVFENLKELLKAKNAAHESMFKFHWKKLPPF
                     NLIWPQVDFLRIIRLMGEIEKHAEEQKVFIKKNLSKATEKELPFFKAIPDYLDALVVS
                     CRALTEIAQFKQDKLEKKEGRSVFSFSKLLKSYENAQDQLVRKGAFVQVHWMEIN"
     gene            19284..19793
                     /locus_tag="PLT31_00880"
     CDS             19284..19793
                     /locus_tag="PLT31_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93718.1"
                     /translation="MKIIFISNVVVMIALIVIMLTLKDGYKTQALTVVRQSTKAYTDE
                     VNKTLFAQGQFVDEWNLVWELVFETVNSSDRSLAAFDKKVTELESTYLADAKNKEKLE
                     KWGLKELKLNITSEKTKRVISLAGNPVVTFDFTGGSFKSVDYKALRSVSPSEMFKIKE
                     PAVFNFNAQ"
     gene            19809..20306
                     /locus_tag="PLT31_00885"
     CDS             19809..20306
                     /locus_tag="PLT31_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93719.1"
                     /translation="MNFKKLTILSIVMSVALLVTVFLQKGSYVSQAKEAYEKSTKKTF
                     SNASYVIGAVNSVIENNSLLWEIACDASQQAKNSKDFALIEKRKDTGKILLSPDTKTD
                     KETGYLTRKVAWNSDYYIVASFNKKNNALVGINVEALLGRVKEDTSNGFSDEDLQANE
                     EAAEE"
     gene            complement(20487..>21532)
                     /gene="purF"
                     /locus_tag="PLT31_00890"
     CDS             complement(20487..>21532)
                     /gene="purF"
                     /locus_tag="PLT31_00890"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPW93720.1"
                     /translation="APDLRAEMEANGHIFQTTSDSEILLHEIARTPAANLSEAIKKAV
                     SKFTGSFCLILLTVDTMYVARDGFGIRPLAMARMGKSWCIASETCAFDLLGANYVRDI
                     QPGELLTINKDGIHSEQFTKKDRKAHCIFEYIYFARPDSKIFEQSCDKIRRKMGKQLA
                     SECPVDADIVIPVPDSSTTAALGYAQASGIRFEIGLLRNHYVGRTFIDPTQNVREQKV
                     KLKFNPIEGVLKNKRVCVVEDSIVRGTTLKILCRMLRNAGALEVHIRIASPPVTSPCF
                     YGMDFPSPGELAASSMTPLEIAKMLGVESLGYLSVDGMKACTGQPADYCAACFDGVYP
                     DFTGNDSTKTRCG"
BASE COUNT         6432 a         3857 c         4180 g         6963 t
ORIGIN      
        1 caaaagcttg ggttaagtct ttgttcgctt tatttatagg tgctttatta ctaagtggtt
       61 ttgctgtatg gattgaagta agccttttaa gttgggcctg tttactgata ttattatttg
      121 ccgatgcttt aaccgtcggc tggtatactg gagtgcatct aaaagaaggg aaacctaaag
      181 cgggctctat cattcaagca tccttaaaac aacttccagt gattgcaata ggggcttttg
      241 tggctcttgt gatctcttta tttgttgaaa atctttggtc gttagaagcc gttctttccc
      301 aatctctttt ttggatggct tatctagggg tattttattt aatattttcc ccttattatt
      361 cttttatgtc tttagatcaa ttacgctctc aaaaacgtca agtgaaaatt ccgaattcag
      421 gtgcatctaa acgatcatag gagttttcaa acatttattt tgcataagtt gtaagcgtag
      481 atatcaatta atcttgaggc ttaaaataaa tacttcacgt aaaggccgaa ggattccatt
      541 gttctcgaca ttggctcaaa gtattcgcct tcataggcag caatcttaaa atagaatttt
      601 aagttaagcc ctaaattaat gtgttttgca agaaagaaat caatattgga aatattaaaa
      661 ccaacaatat cccagccagt cgctccgtaa tgcggtgctg aagaaggcat tgtttttaac
      721 agaaaaatat tagtatggtt tgaaatgcgt ccgtatttgg ctgaaatatc aaaagcggct
      781 ttatagtgta ctccataatt caattggtat gttcttgtcg gttcataatc aggataaaac
      841 tcttttaaaa gatcatcgta attaaagtct gtggagccta gaatgacaaa ggatggaatg
      901 cttgttaacc taaaagaaag agagggacga agccaaacat tgaaatcact accaaggccc
      961 agagaaaaag cactcatctc gactaaatca ccgtagaata catcatagct agtgtatgtc
     1021 gcaaaatcaa accaatcttt tcctgaaacc ttattgatgt tttttaattt ggcaagaact
     1081 tctaaattaa ataaacgttc tcgttgtccc atttcttgag tggcttgaat cgtaaaatat
     1141 tcaaagggct tttttaatac ccgattagga gatccataga ctaagtttag accagcaaag
     1201 ccaaaaagct cttcccattt gtcatccaat cgatcagcgg gagatttgtc gtatcgataa
     1261 tcactaccaa aacgaaatcc agcaccagta aataccgaga gctctattgg aaaataatga
     1321 ggatgattgg gtctcgtacc agttattctg cggtgcaagt aggtgtatgg atttaaaatg
     1381 aaggctccag cgtgatctat ccaatttggc tttggtttaa ctaacaagcg ttctgatagt
     1441 cgatacagaa tttcaccata aaaagcacca ccgtagcttg tcataataaa atcatttaat
     1501 gagggatatt cattttcaca aaaaaattcc catgagaatg atccaaacat atcaaataaa
     1561 acgctttggt agaaggaata tccagaagct cttgcggcag catgataaaa aacgccttga
     1621 tatgggtgcc cgaaaaagtt gattccaaag tggttgttgt cccaagtcca gccatttttt
     1681 atgtttctct tccatacttc gggactaatt tttgcgtaat ttacccctag atgataacga
     1741 tcccatgccc agacaaaaac attttgaacg agaatttcac cgaaaggaat taaaacaggt
     1801 aatggctctt tataatcgac agaaacggtg gacggggcat tatagtcaaa agtagagtca
     1861 agcggaaaag aagctctacc cgcataagcg tttcccatag caataaaaag aaaacaaaga
     1921 agtgaaacaa ttatattctt cacacgtgaa aggtagtttt atttctgcaa aaatagcaga
     1981 tagaaatgga taagaggttg ttttttatgg atgaaaaggg tttgtaatgc tttttttata
     2041 aaaacggctt ttcaataatt tttagaagct cccttgcgcg aggcttaaaa tcatttaatt
     2101 cactacgtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc gaggcttaaa
     2221 atcatttaat tcactacgtt caacatgatt ttaatcgaac tcacggcctc ttgctcccga
     2281 agcaagcgct ctaccaggct gagctgtatt ccgttttatg gggagtaatt ctagctaggc
     2341 tttttgaaaa gaggtgcttt ttcaatatcg gatttaatct ttaataagct tgagccaggg
     2401 ttatttcgtt caaggccgta ataagctaca agatgcttta atcttgtttt aatatgatgg
     2461 tccacaaatc gttttacatt gccgtatttt aaatttccac cgccgtggat aattcgaagt
     2521 actttatatc ccgcagggat catggcatgg ataagactat ccactttttc taccgcttct
     2581 aacccttcgc aaccatgtaa atcaatctct tgttctggct caccaaaagg gttatcttgc
     2641 aaaaatttct taatcttttt cgaattcgtg cgcttggttc gagtcaaacg tttcgttgat
     2701 ttctgctcca gatctttgtc tatcatcgga tgcgtctgaa tccacgcaag ctgcatttcc
     2761 tcttcttcat taaacgccat aatcatctac tctaaagttt ctatgataat tccatgtcca
     2821 ttgttcagga tgctcttcaa tccaagattc tatttcatta gaaatccctt gtgtgatagt
     2881 attggcttct gattttggag ggtaaatttt ttcgaaacgt acaataattt gattttttac
     2941 agaaaacgtt ctgaatgtga taattgcggc tcccatttgg tttgctttaa taggaagccg
     3001 aaggtataaa ttgaaatttt tatcccataa acgagtcaga acgcctttcg aattttttgc
     3061 ctgatctact aacatcgcaa gaattccttt ttcacgaata agttttttta gaactaagtg
     3121 tgtgtcatca ggggaatact ctttcaaatg agaatggctt cttatttttt ttagagcttg
     3181 ggttagtgag gtgtcagaaa atggatttgt taataagtaa atcaggcgat gatatcggca
     3241 aaggcttctg tgtaatagtt caaaaggccc ttgatggaca ctgatcgcga caatggggac
     3301 gttttttttc acaagatctt gaatgagaaa ttcgttttca taaaagatat tgcttagtgt
     3361 ttttttatgc ccaagtaaac tgcgataaga gagaatggca tttttataaa gactataaaa
     3421 aacggctcta ggatttgttt tagtatcaaa agaagtctct tttagtaatc gctcgactcg
     3481 accccatgcg cgatttcgat gcgtgaattt atataaagga taaataaatg caaagactat
     3541 atcaaaaaaa aaattaggcc atcgaatcca atggaataaa aacaaataat agagctgctt
     3601 tctcataata ttttagggat caaaagcgag aatgttttta cctaattctc tatcgaacac
     3661 tgcttgagaa agagattctc cgccataaga aatagtaggg gatatttcat acaattttct
     3721 ggattcgact gtaacgcctg cttgttgaag ccagtattta tggagcatcc caagcatcag
     3781 acgtgcagac tttggagaat catctccatc ggcatttttt acaggagcaa attcatcttc
     3841 tctagagacc ccatacaagg tcatagaacc tagtaatgga aatgcatcga agagaaactg
     3901 ttcaaatttc caagcatttg caacgccaaa aactgttttt ttagctgagt gccatgggag
     3961 aggtgtactc tgtaatttct ttaaggcttc aattctaaat aaatggatcg caatatttgc
     4021 accatcaaaa agaggagttc catctggata cgtttctaag cgaagtgcgt ctggaagatc
     4081 actatattca atgacgcttg gttttttatt tttaacggca aacacgccta ctttttcttg
     4141 agggtgtttt ttacgaacca ctttagacga agaaagggcg cgcccgtcac tcgcgagagc
     4201 acccacaaaa gcggggtctg caatacggac taaaacgtta tcgacgctat acaagaatac
     4261 aaaacgaacg cctttttcaa tgagccaagc gagcgttcca ctttgtgcaa gagctctaaa
     4321 acagccacca ttgccatcgg gtactaaagc gagatgttct ggcgagcttt taatggcttt
     4381 tccgttagga tcgagagcgc acatggttcc ttgagaaaag aagcgaatgt aatctcgagg
     4441 gagaccaaaa taatgattct cttcaaaatg agcaatggta tcttcatgat tcagagggct
     4501 tgtcatgata caccaaggaa tcggatgccc tgaacgagat gaaagattgg aaatgcgttc
     4561 ggcttgaatt tggaaaaggc ttttaccgct tggtaaacca atattataag ccccttttgg
     4621 gccttcaaaa ccaagacgag aaccctgacc accagccact aaaaatgcag cgacttgccc
     4681 tttttctaaa agaagaaaac cagtttcctc ccacatggct tgcttgatat tattttctgc
     4741 taaatcaaat gtcagaggaa gaatttcatc ttcagtagaa atgactgggg tagaggaatg
     4801 ctctttaaaa agatgattta aaagattaaa atcttgagag gcaatttctt tttctagttg
     4861 caaacgatcc gcttctgata acgagtcgat attatctaaa agatgctctt gccccaattc
     4921 cgaaagagtt tttcttaaat ccatttttta cctaatgcca aagaaaatca ccatgcctac
     4981 gattaaagca aaaatagaga caaggtgcat gcgccaagct atttttgagt tgagcaatga
     5041 ttttgatgaa aacttatcac tccatttttt ctgataatga tggtgtaaag gagcgcatag
     5101 aaaaagacgt ttccctgtgc cagttgtttt ttttgtgaat ttaaaccagc taatttggaa
     5161 aataacagag aacgcttctg caatgataat aatcccgatg ataggcaaaa atagaccagc
     5221 ttgcaccaaa ataaacatga tgccaatcgt tgcaccaaag cccacagaac cagcatcgcc
     5281 catatagatt tccgctggag gactattaaa ccaaaggtaa gcaagcaaag accctgcaat
     5341 cgacattgct aaaataaaaa gttcatcaca accaggaagg taaggcacat gcaaataaga
     5401 actaaaaata aagttaccac tcacataagc cacaagccca acaaaaacca tgcttgtcaa
     5461 aataggaaca gaaactaaac tatcgagccc atctgtaaaa ttggttccat tggcggtagc
     5521 tgcaattaca aaagtcataa agccaataaa cgcccagttc gggagttgta tttggaaaaa
     5581 agaaataggg caaaatggaa cagttaaatt accacgaaga tcaggaataa atttatagca
     5641 gaaaatagca acaacaaaac taaataagaa atatagagcg agacgtattg tgctagacaa
     5701 gccatctgct ttatccatat aatcagcggc tttcaatttc ccttgaacaa taagacgttt
     5761 ggctctcact tttgcaatat catcaatcgc gcctacagaa gaaaataaaa ctaaaatgac
     5821 aagagtggag attgtatagc cgttcatttt acatgtgaaa agagaaacaa gtgtcaccac
     5881 tagaacaagt aaaagaccgc ccataattgg aggagatttg cttttgccat ctttatcaag
     5941 atctgaagtc gcatcgagac cttgcaatag acgaatatac tttggcataa aagttaaaac
     6001 tagaataatg gataaaagag ccgcaaaacc agccctaaat agacgtccat caaacatttc
     6061 aacaccggta gtatgatata accattgaaa tagcattcta aatctctaaa aaataaggaa
     6121 aaggacgctc ttaaatttaa taaaaaagta ttagtaataa tatatgtgta tagagaatag
     6181 cctctttttg acgtagtcat gataaaatga gaaacacttg cttaattatt ttggtagtaa
     6241 ttgtttcaca tttgaaaaaa tggttgatgc aaaaacgctt atcgcagaag ttttaaacga
     6301 aaagtgaaaa taatacaata aaaaaaggag actttcaaag tctccttttt ttacgatttt
     6361 tggggttatt ttgctaaacg agcgagtagc ttattgattg cactcacgta ttcgcttggg
     6421 tttggaagag gggagccttc tgcaaggagg gcttgaccat aaagcgcctt tggccaatct
     6481 tctaaatctt cggctttgtc gattttagct tttagcatct cacaaatagg atgtgttggg
     6541 ttgatttcta aaatgcgttt cgacttttcc attttgcctt gtcccatcgc ttccatcatg
     6601 cgctccattc tggagctcat ggcgtcttcg cttgtgacta agcagcatgg gctttctttg
     6661 aggcgactag aaatacgaac ttccttaata tcagaatcaa gtgttttttg tagagcttca
     6721 caaaggccct tgaattcgga cttagaggat tcttcggctt tcttttcgtc ttcggtcttt
     6781 tcaaagtcaa cgtcaccacg actaatatca tggaacttct tttcgctaaa ttcagttaaa
     6841 gaagacatca tgaattcatc aataggatcg ctcattaaaa gaacttcata acctttgctg
     6901 cggaatgctt ctaataaagg attggagtca attccttctt tgcgatcacc aataaggtaa
     6961 tagatctctt tttgagtttc gggcatacga gaaacataat cagtaaggct aatgagagaa
     7021 ttcccttctg ttttggtgct acggaaacgg agcagctttt taagctcatc aaggtgttcc
     7081 cagttcatgt agaagccttc ttttaataca gctcctagtt cacgccacca ttctgtatat
     7141 ttctctacat ctttgtctgc catttgttgt aaagaatcga gaactttttt tatgacgtgc
     7201 ttacgaatat tggtgatgat tttattggat tgtaaaatct cacggctcac gtttaatggt
     7261 aaatcttcag agtcaacgac gccacgaaca aagcgtagcc atggaggaag aagctctttg
     7321 cagtcatcca taatgaagac tttttttaca tagagcttaa ggccgtgtgc gcgttcatta
     7381 ttccaaatat taaagggtgc tttggatggg atatacacaa ggctccagaa ttccatggat
     7441 ccttcggcat gggagtggtt ccaagcggct ggttctgttc cttcgtgagc tacgacatta
     7501 taaaattctt tgtattgttc ttcagtgact tctttttcgc tttgacgcca taaagctggt
     7561 ttttcgttta agcgttttgg ttcatgaact tcaatttcgc ctttatcatt tttagttggt
     7621 tcagggtgaa agaagatacc ataagaaata aattcactgt acttttgaat gacactcttg
     7681 atttgccatt cgtttgcata ttcaaggctt tcttcatcgt ctttgaggta aatggtgatt
     7741 ttggtaccaa catcttgttt tggggcttct gtaatttcga aatcgccagt accttcagat
     7801 gcccagagga aaccttgagt ttcacctgct ttcatggtca aaacttctac tttgcgagcg
     7861 accataaata cagaataaaa accaactcca aattgaccaa ttaaatccac attggattga
     7921 tctttttctt tgagagactt gatgaaattc ttagttcctg agcgtgcgat ggtgcctaaa
     7981 ttgctgatca aatcttctcg attcatgcca ataccattat cttcaatggt gatgatttta
     8041 ccttcggtat tgacggcgat atcgattttt aaggtagttc ctacagggag aagggctgca
     8101 ttagaaaccg ataaaaaacg gcgtttgtct aaggcatcag ctgcattaga aacgagttct
     8161 cgtaaaaaga tttctttatt gctatataaa gagtggatca ttaagttaag aagatcacgt
     8221 acttcggttt ggaattccat tttttctgtt ttcattatat ctccgtatat acaatggtag
     8281 gtttgaataa actttattta gattttcagc ataaaaatag tgaaaaagaa gtgccctgac
     8341 aagtaacact acctttttaa gaaattagtg atctaacaat gcttgggttt ttactaattt
     8401 cagttttaat cacccctttt atggactctt tatgctaatc tctgctattg ttgctatctc
     8461 agaaaataat gtcattggtc gtgatggcca tttgccttgg cgcttgccag cggatttgaa
     8521 atattttaaa tcggtgacga cggggcatca tattatttta ggccgcaaaa attatgccga
     8581 tattggaaga ccattacctg atcgagtgaa tgttgtttta actcgaaata atgcttttag
     8641 tgctccaggc tgtgttgtgg cttattctct tgaagaagcg ttacaaattg caaaagacgc
     8701 gggtgaaaca gagtgtttta ttgtgggcgg agcggctatt tataaagaag ctttgccttt
     8761 ttgcaaaaag ctttatataa caagagtttt agcatctatt gagggtgatg ttcagatgcc
     8821 ttctttagga gagggctggg ttcttcgaag cgaagaaaaa cgagacgcag acgagaaaaa
     8881 tgcataccca atggtttttc aggtctttga acgcgagtaa gggccacaca tatcgttgtt
     8941 atttatagtt gtattttatt gtctcaaatt ttgccgataa tgcgtcgaaa tggtctacaa
     9001 aaaagccaat aaaaatgcag cttattcgat attcatcact tgttcgcgaa tcgtttcgac
     9061 agcatctttt aattgaatag cgcaagcagc aatttgagca cattggcact tggtgccaag
     9121 cgtattggct tcacgaccca tttcttgtaa aataaagcca agttttttcc cttgggaacc
     9181 accagtttta agggtattca agaatagttg attatggctt ctaaagcgaa ttatttcctc
     9241 tgtaatatcg agacgatcgg ctacaatact tgcttcttga atcaagcgga taggatctag
     9301 ttcgaggttc tgcaaaaggc cttttaaacg ctcttcaaat ttttctttcc agtcgcaaat
     9361 acgtgttggt tcaagtcttt caatttcatt gagaatattt tcaattgtag aaatacgggc
     9421 tttcagatcg gtaacaaggt tttcaccttc ttttttgcgc atttcaatca tttggtctat
     9481 tgctaaatct aaattcaatg cgatatgctt ttctagttct tcttgatctt gcgaagaatc
     9541 ggaatattga atcaaatcag gaagagagag caaatgttct aaatggacat tgccttcgat
     9601 attaaactta tctttaatat cattagcaat atcaataaat gctttgactg cggcttcatt
     9661 gtacgaggta gggatatttc ctgtttctcc agaatccatt aaaatgctta aaaagacaga
     9721 cccacgagct actttgtttt taatctgatt acgaagatcg ttttcaatat ggcttaagtt
     9781 tttagggatt tttgtggtga tatctaggta acggttattt actgaacgta attcgattac
     9841 gatagtatgg ttattaaaag cagtttccac tttaccatag ccagtcattg agaaaataga
     9901 tgccatttta taaacctttt ttatttgtct tttttggata caagggctac cactctttct
     9961 gcgccaagat tagtacgacc aatagaggtt agtattttac taaataattt atcgttatca
    10021 aaatagagct tttcgatggt taaattttcc gattcacaca tacggataaa gtcacgtagg
    10081 gtaaataagt gaatatttgg agtattatac cattcatagg gaagttcgct tgatttgggc
    10141 atacggccaa aacggcaaag tgctgctcgt acagaccagt gaccaaaatt tgggaaggta
    10201 atgaccgctt tacctgcaac acgtaggatc tcatgtaaaa gatctaaagg catgttgact
    10261 tcttgaagtg ttctattcaa aatggcaaaa tcaaaactgt gattcccaaa atctttaaga
    10321 ccttcatcga ggtctttttg aatcacagga acgcctttcc ttaagcagct taaaatactg
    10381 ttgaagtttt tatctactcc aaggccttga acatttttcc gaattttaag acgttctatt
    10441 aagtccccgt tcccgcaacc taaatctaaa atacgtgagt tattaggaat cgccgtttct
    10501 aaaagatcaa aatcttcggt gtcatgaaac actggaatcg cttgcgtatc tgaatgttgg
    10561 tttaatttag ggtctggaga aataaaggca gaaatagctt ttcctaaatc tcctacttca
    10621 agtaaaaatc catcgtgacc aaataaggta tcgagttcaa ggcttgtgac ttctttttct
    10681 gcgttcacta aagcgtgtgt aatctttctc gattcatgtg gagggaaaag ccaatcggtg
    10741 ctgagtgtaa tatttaaaaa tttcgcattt gtcttcttaa atgctttttc taaagaacca
    10801 tattctgttt caagatcaaa agtatcagtg gcataagtga tatgcaaata actatttgca
    10861 tcaaaacgtt ccacaaactt agccccttgg taggctaaat aactttcaag aggaaagccc
    10921 attaagaatt ctttgggatt aggatctttg catgcttctc ttgtgaattt ggcttccatt
    10981 cccactcgag aaaggtatgt caagtgtgca atttttcttg cgttggaaag acctactgca
    11041 ggaatttctc cctcgtagta gtctccgcca ttaaagttag ggtcttgttt aatggcatct
    11101 cttcccacga tttcaaaacc aagtgcttgg ctagagaagc gaggagagga ggcgatgata
    11161 atgcagcgtt ctacttcttc tgggtagaga atagaccatt tcatggcctg aaaaccaccc
    11221 atggaaccac caatcacaca aaagattttt ttgattccaa gaccatcggc taatttcttt
    11281 tgcgcttgca ccatgtctcc gatggtgata ttaggaaaag aagctccgta aggctttagc
    11341 gtctctgggt tgatgcttgc agggcccgta gtccctttac atccgcctaa aatattactg
    11401 caaacgacaa agaaatgatc ggtatcgatg gcttttcctt tgccaatgag agcatcccac
    11461 caacctactt ttttatcatc aggtttataa aagccagcga catgagcgtc tgccgttaaa
    11521 gggctacaaa tccaaatgac attatctgct tctttattta attcaccata cgtttcatat
    11581 cgaatcgata actcttttaa tcgaccaccg ccttccagca aaaatccctc tgatcccaat
    11641 tcaattttca tgtcttgagg gacaactgga ccaaggctat ctttatgcaa aaattcactc
    11701 atttgcataa atatagaaag aaaatagagg ggcagaattt tttttcaggg cctttttgct
    11761 attttgattt tcatgtcaac gtcttctatt gttgtgacac cacaaataat tgaattccct
    11821 agaattttag atgaaagggg gaatttaagc tttttggaat cggagcgtca tctttctttc
    11881 aaaattaaac gctgttattg gatatacgat gtccctggtg gagaacttag aggtagtcat
    11941 gcttttaaaa atcaacatga agttatcatc gctttatcgg gtagttttga cgctttaatt
    12001 catgatggtg tagaagaaaa acgcttttct ttgtctaggt cttattacgg tttatatttg
    12061 cctcctttgc attggcgtac cttagataat ttttcaacca attcgcttgc tttagtgttg
    12121 tcttcaactt cttacgatga agacgattat gtttgggatt ttgaagaatt cctaaaatta
    12181 aaaaaaggat cttctaaatg attaaaacaa ggttaagcga ttgctcgtta atcaccctcc
    12241 ctcgccatag cgaacgatcg gggagtttaa ctgctgtgga atcagataag ccaattcctt
    12301 ttgatatagc aagagttttt tatctttatg atatccctgg aggagaatct cgaggtggcc
    12361 atgctcatta tgaatgccat caattactca ttgccgcaag cggtgctttt gatgtaaaaa
    12421 tggatgatgg tacttcttct aaaatttatc gattagatcg cccttattat ggattacata
    12481 tcccacctgg aatctgggcg gaagaaattt ctttttcatc tggttctatt tgtttggtat
    12541 taacatctca tcattttgat gagttagatt attgccgaga ttacacttgt tttctggaac
    12601 aaaaaaaaga ttaactatgg gacatctaat aacaaatggt atcgaatgca tacaagtatc
    12661 ggctgcggat tacagtcatt ttttaacatc agaaactatt gtttatcaat cagttgcatt
    12721 taatgaatta aatgcaagta aagtaagtca gttgcgttat ttacttttta aaagagatgg
    12781 aaaagcaatt gctggattga ttgttggtga aaagggaaat gtttggctat ctccattttc
    12841 cgctcctttt gctggtttta gcttttctaa atcattttca ttaaccactc ttgattctat
    12901 agtgagttgt ttatttgact atatcaaaaa gctggagatc gttttacaaa ttacaatgcc
    12961 acctcttttt tataagcaag acttatatct taaactcgct tatgttttac aacgctattc
    13021 tacttcttct tatacagata ttaattacgc attagacttg aaaacggatg attatgattc
    13081 cattttaata agtacatcac gtagacattt aaatagggct aagagtattc cctatgtatt
    13141 gaaacaagtg gatactttag aagaaaaaaa actcgcttat gatgtgattg ctcaaaatta
    13201 taaagccaaa ggctatccct taaaaatgag tttgaatgat ttagaaaaaa caatgacttt
    13261 tattcatggt cgctattttt tactttctat ggatgagatt cacgtggcat cagcgattgt
    13321 ttatgaagtg accccgacaa tagctcaatt gatttattgg ggagatcttt ccgcttattc
    13381 tttaaaacgc ccaatgaatt tattagcggc agaattattt gagatttata aaaaacgtgg
    13441 ttttgaattt ttagatattg ggcctgcttc agaattagga gctcctaata ctggcctttg
    13501 ttcttttaag gaaagtattg gttgctttgc cgatttgaag tatcatttta cattgaaggg
    13561 gaatacataa tatggttcgt tttttagacc tacaaaaatt aaatcaattt tatgagcctt
    13621 ctttgtctca ggaagccaaa aaaataattg attcaggctt ttttattcga ggtcgagctt
    13681 gctcagaatt tgagtccgct tttgctttat tctgtggcgt tccatattgt attggggtcg
    13741 gaagtgggtt agatgcatta actttgattt tcaaagcgta catagaactt ggccgtatta
    13801 aagcaggcga tgaagtgatt atccctgcaa atacttatat cgcgaccatg cttgccgttt
    13861 cagaaacagg gctagtgcct gttttagtcg agccagacga ggagtctttt aatttagacc
    13921 ctcttctttt agaaaaagca ttaactccta aaacaagagc gattttaccc gttcatcttt
    13981 atggtcggtc tgcaaacatg aatccgattc tctcttttgc aaaagaacat caattattag
    14041 tagtagaaga tgctgctcaa gctcatggtg catattatgg aaaaaatcgt gtgggctctt
    14101 taggggatgc cgctgccttt agtttttatc catctaaaaa cttaggcgct ttaggagatg
    14161 ggggtgctgt aactacaaaa gacgtctctt tagcgcaagt ggttcgaacg ctcagtaatt
    14221 acggagcaca aaaaaaatac gtgaacactt ataaaggagt caattcacgt ttagatgaaa
    14281 ttcaagcggc tttcctttcg gtgaaattac cttacttgga acgtcataac gctcgcaggc
    14341 gttcgattgc ggaacgttat ttaaatgaga taaaaaatcc tttgattcgt ttgccacaaa
    14401 tgggagtcag cgaagaacat gtttggcatg cctttgtttt acgaacagat tatcgagatg
    14461 aattcataaa acatttacaa aaccgtagca ttgaaacact cgtttattac cctagtgctc
    14521 ctcatcaaca agaagcatat aaaactggtt tagtgtatga gtctttgcca atcacagaga
    14581 gacttgcggc tacagtgatc agtatccctt taaatccaat tttaacagat gatgaagtca
    14641 cagaaataat tcaaggagtg aatgattttg tttgttaaaa tttttcgaca cgcctatgct
    14701 tttatgaggc ggcttcttta ttctttttta tcagaagcat cttatgaagg taagccaatt
    14761 aaaggagccc ctctactcac gcaagggaag gggcgtattc actttggttc tggcgttcgt
    14821 ttaggttgga ttacaggaaa gtctttttgg aactcttata ttttttgtga tgcacgtaca
    14881 agttcttctc aaatttcttt tggaaaagaa acttggatag gaaatgattt ttctgccatt
    14941 tcagaaggcc ctggtatttt tattggagaa cgagtcttaa ttggatctca tgtggatatt
    15001 tatgacactg attttcatcc gctttctcct gaaaatcgtc ttggtggaga acctaaaaaa
    15061 ggggctgtta ccattcaaga aaacgtttgg ataggcaatc gagtcacaat cttaaaaggg
    15121 acaaccattg gtgcacatag cgttgtggca gcaggttcta tcgtctctgg cacatttcct
    15181 gaacgagttt tgattggcgg agtccctgct cgagttattc gttcgctgga gtagtcgttc
    15241 atgaaaaaga tgcctcaagt catcaagcaa tcttttttat ctgggatggt cgcattcatt
    15301 cgtattgtaa catcttttgc cacaaataaa attttagctg tgattctcgg gcctgtgagt
    15361 tttgcgatgg tagggcagtt tcaaaattta atgatggctg ctcaggcagg aggctcttta
    15421 tccattcaga atggttgggt gagcttaacc tctcaatatg cagaagatga agatcgtcgt
    15481 ttttctttat ggcgctctgg tttaattctt tctgggataa gcattctatt tgtttccatc
    15541 tttttattga tcgcctcttg gactggtgct ttccattttt tatttcctga cgtttctgaa
    15601 agaatgctac gaattgcttt attgtgtgcc attccaggcg tgattgggat ggttttgttt
    15661 tccattgttc agtctattgc aaatggtttt tctgcattta aactttgggc ttttctttct
    15721 attagtactt atctcacaca ggcactttgg gtgattctct ttgttttgtg gaataaagat
    15781 cttattttaa ttgtactagc gacgcaatct ttagtagcac tattcatttc attatttatt
    15841 gcctacaaga aaaaaatctt ttctcgcctt ataaaaagta cttcctttgg ttataatgtc
    15901 tggaaaaagt atgctttgat gggaatcatc ccgatgattt taacgcctat cgttttaact
    15961 gctattcgtt ctcttattgg tacatcaatc ggatggcatg aggctggctt atggcaaggg
    16021 gctttccgaa tctctgactt tttcaatgtg gctttttctt ctgtcttagg cgttatttta
    16081 ttacctaaac tttccaagtt gaaaaatgaa aaatcattct ttaaaacact ttggtacgct
    16141 ttattaggtg tacttttggt cgcttttatc ttaatcttca gtatgttttt tcttaaagaa
    16201 tttgtgattc aattcatttt atcagaagag ttcctcccag tcgctaagca aatgcaccta
    16261 caatggatcg gagacttttt tcgttgtggt tcttggtgtt taggcatagg actaattgct
    16321 aaacaatcag ctaaggcttt tctattttta gaaattcttt cacagagttt attttttata
    16381 ctttctttta taggtatttc ttctttgggt attacagctc cttttggcgc atatgccata
    16441 gaaaacgtac tttgctttat ttttatttta ttgttcacta ttaggaatca ttatacatgg
    16501 aaaccccttt agtctcagta ttacttgcaa gttataatca tgaggcttat gtcgaagcgg
    16561 ctattcgctc gattctttct cagcaaggcg tttcttttga attaatagta attgatgatg
    16621 gaagtacgga tcagagccct ttgattcttg aatcgctttc aaaaaaatta ggcttccagt
    16681 ttatcacaag acctaatcaa ggctttgtat ctacaatgaa tgaattagca aagcttgctc
    16741 gtggtcgtta tttctgttcc tttgcaagcg atgacattat gcctgcaggg cgcttgatga
    16801 ttcaatctac ttttatggaa gaacacccaa acgttcctgt ttgctttgga caaacaatgg
    16861 agatgaatgc ctctggaacg ttagaactaa acttagatga acgtttctta aaaggggttc
    16921 cagaggtcac ttttgaagaa ctatttttag ggcaaaaagc cttacatggc tgtacagaaa
    16981 tgattcgccg agatttattt tttgaagttg gtggctatga ttctcgcttt ttctttgaag
    17041 attttcccct gtggcttcgt ctttcttacc aatatgggcc attacctgtt ttagaaaatg
    17101 tattttgtta ttaccgattg catggaaata acatgcattt aaatcatgaa aaaatgtatt
    17161 cggaactcct aagaattatt gatttgtatt cagagcatcc tctttataag agagccgtgt
    17221 ggcattgcaa atcgaattgg ttttcttcgc tcacttattc aaataaaatg aaggcccttc
    17281 agatgttttt taagttagct tctccttcat ggatgttctt acgaagagtc ccaaaattat
    17341 ttattccaag acgctttttg aaatattaag aggtgattat gatttcggtt tgtatggccg
    17401 tatataacgg agaaaagtat cttcaagaac aaatcgaaag cattttaaaa caattgtctc
    17461 cagaggatga attagtgctt tctgatgatg cctctacaga tgcgtcgatt gaaattatag
    17521 aaagaataaa agattctcgt ttgattttat taaagaatga aaaacgtcta gggcctattt
    17581 ataatttcga acgcgcctta gttcattgcc gaggcgatct gattttcttg agtgatcaag
    17641 atgatctctg gctcacaggt aaagtgaatg cctgtgttca gaaactaaaa gaatatgatt
    17701 tgattttaca tgatgctttt gttttaagag aaaataaaag gcaagaaaaa actctttttg
    17761 aactcagaaa cgtacagcgg ggtttattca aaaactggtg gaaaaattct tacactggtt
    17821 gctgcatggc ttttaagcga accattttag agtccgcaat gccttttcca aaaaaaatac
    17881 caatgcatga tcaatggatc ggaatttgtg cagaaaaaaa ataccgcgtc ttatttctga
    17941 attcttgctt tattgaatat cgagtacatg ataataatgc gactcgtatt acaggaacaa
    18001 aaaaacagac tttcagacag cggtttttgt ggcgttacta tttacttaaa gcgcttttga
    18061 gtcgcttttc tacgcattca taattcacaa tattccaaaa agccgaaatc cattccgcac
    18121 ggcgattttg gtattttaaa taataagcgt gttcccaaac atcaatagtc attataggtt
    18181 cataacctaa ggataaaggg catatttggt catgggtgtc gatgatttcg agtgttttct
    18241 cttttgttaa aacaagaaaa acccagccac ttccaaaatg agctagagcc tttgctgaaa
    18301 aggcttcttt aaatgcatca aaagaaggcc aatacttttc tatctccgct tttaagaaag
    18361 ccgaaggagt tgtcttttct gcaggagaaa gattttccca gaaaaagttg tggttaaaat
    18421 aaccgccacc atggttgatg actcttgtac gtagagccat gggaacagaa tccagattct
    18481 ttaaaaggct ctcaacggag gtggtctcca ctttcccttc ctcaagcgct ttatttaggt
    18541 tatcggtgta ggcttgatga tgtttggagt ggtgtactct tacggtagct tcgtcgtacc
    18601 acggttcgag tgcatcataa gcaaagggaa gagtaggtag tgtaaatgcc ataaaagcgc
    18661 tccttgtgtt ggtttgttat cgttattgat aaggttagtt tatttccatc caatgcacct
    18721 gaacaaaagc tccctttcga actaattgat cttgcgcatt ctcataagat tttagtaatt
    18781 tactgaatga aaaaacagaa cgaccttctt ttttctccag tttatcttgc ttaaattgag
    18841 caatctctgt taaagctcgg caagaaacga ctaaggcgtc taaatagtca ggaatggcct
    18901 taaaaaaggg taattctttt tctgttgctt tagataagtt ctttttaatg aatacctttt
    18961 gctcttcggc gtgtttttcg atttctccca taagtcgaat aatgcgtaaa aaatcaactt
    19021 gtggccaaat taagttaaat gggggcagtt ttttccaatg gaatttaaac atggactcgt
    19081 gagctgcatt ttttgctttt aatagttctt taagattttc aaagactata gaagcaaggg
    19141 attcatcttt ttgattttcc tgtgtcatat gatttcaaat atacatttta ctttaaaaaa
    19201 cttgattatt tgcttgtaag gatttagatt ctctgagaag attgtttcac ttcttgaaca
    19261 ggaatgtatt tgcaatgaac aaaatgaaaa taatatttat aagtaatgta gttgtgatga
    19321 ttgctttaat cgtcatcatg cttactctta aagatgggta taagactcaa gctttaacgg
    19381 ttgtccgtca atcaacaaaa gcctatacag atgaagttaa taaaactctt tttgctcaag
    19441 gtcaatttgt agatgaatgg aacttggtat gggagctcgt ctttgaaacc gtcaatagca
    19501 gtgatcgctc attagccgct tttgataaaa aagtgacaga gcttgaatcg acttatcttg
    19561 ccgatgctaa gaataaggaa aagttagaaa aatggggcct aaaagagctc aaattgaaca
    19621 ttacttctga aaaaacgaaa agagtcattt cacttgcagg gaatcctgta gtgacatttg
    19681 atttcacagg tggttcattt aaatcagtag attacaaggc tttacgctct gtctctccat
    19741 ctgagatgtt taagattaaa gaacctgctg tattcaactt taacgctcaa taagctcggg
    19801 agaatccaat gaattttaag aaattgacca ttttatctat cgtaatgagc gtggctcttt
    19861 tagtgactgt ttttttacaa aaagggagtt acgtgtctca ggcaaaagaa gcctacgaaa
    19921 aaagcaccaa aaagactttt tcaaatgcct cttatgttat tggagctgtt aatagcgtga
    19981 ttgaaaataa tagcctatta tgggaaattg cttgcgatgc gtctcaacaa gcaaagaact
    20041 ctaaggactt tgcgttaatt gaaaaaagaa aagatactgg taagatcctt ttaagcccag
    20101 ataccaaaac ggataaagaa acaggttatt taacacgtaa agtggcttgg aacagcgatt
    20161 actatatcgt cgcgagcttt aataaaaaga ataacgcttt agttggtatc aatgtagaag
    20221 cacttcttgg tcgagtgaaa gaggatactt ctaacggttt ctctgatgaa gacttacaag
    20281 ctaatgaaga agctgctgaa gaataatttt ttaagtttta tttgtcgaac aaaaaaaccg
    20341 ttgaaaggcg gtttttttta taattgtccc aatatttgtc gaacaaaaaa accgttgaaa
    20401 ggcggttttt tttataattg tcccaatatt tgtcgaacaa aaaaccgtcc ttcgacggtt
    20461 ttttgtttta aagaaatgat ttgttattat ccgcagcggg ttttagtgga gtcattgcca
    20521 gtaaaatctg gataaacgcc atcaaaacaa gcagcacagt aatcagctgg ttggccagtg
    20581 caggctttca tgccatctac gctgaggtag cctaagcttt caacgccaag catttttgca
    20641 atttctaatg gagtcattga acttgctgca agttcaccag ggcttgggaa gtccatgcca
    20701 taaaagcaag gggatgttac tggaggtgat gcaatacgaa tatgaacttc aagtgcacct
    20761 gcattacgta acatacggca caaaatttta agagtagtgc ctcgaacaat agagtcttcg
    20821 acaacgcaaa ctcttttgtt tttaaggacg ccttcaatag gattgaattt tagtttgact
    20881 ttttgctcgc gaacattttg tgtagggtca ataaatgtgc ggcctacata gtggttacga
    20941 agaagtccaa tttcaaagcg aataccagag gcctgagcgt aaccgagtgc tgctgttgtg
    21001 gaactatcag gaacaggaat tacaatgtct gcatccacag ggcattcgga agctagttgt
    21061 ttacccattt tacgacgtat tttatcgcaa ctttgttcaa atatttttga gtcagggcga
    21121 gcaaaataga tgtattcaaa gatgcagtgt gcttttcggt ctttcttagt aaactgttca
    21181 gaatgtatac catctttgtt gatggtcagg agttcgcctg gttgaatatc gcgaacgtaa
    21241 ttggctccta ataagtcaaa cgcacaagtc tcggaggcaa tacaccagct tttacccata
    21301 cgagccatgg caagaggcct gataccaaag ccgtcacgag ccacatacat ggtgtctaca
    21361 gtgaggagga ttaagcagaa gctaccagtg aatttggaga ccgctttttt gatggcttca
    21421 gataaattag cggctggagt acgtgcaatt tcatgaagaa gaatttcgga atcagaggtg
    21481 gtttggaaaa tatgaccatt tgcttccatc tcagcacgaa ggtctggagc at
//
LOCUS       DAOOBB010000008        19958 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_3584_length_19958_cov_5.128172, whole genome shotgun sequence.
ACCESSION   DAOOBB010000008 DAOOBB010000000
VERSION     DAOOBB010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 19958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19958
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3584_length_19958_cov_5.128172"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="PLT31_00895"
     CDS             complement(<1..297)
                     /locus_tag="PLT31_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93721.1"
                     /translation="MRKTSPLVALFMGLFYSVSASVTDSLPLQNLGETEVHIGASITE
                     NLSNQVVSISEEAWVGKNFTAIDILSSYGGIQGHKQGGIGSFQSISIRGASSRGV"
     regulatory      complement(300..496)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(537..1883)
                     /locus_tag="PLT31_00900"
     CDS             complement(537..1883)
                     /locus_tag="PLT31_00900"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPW93722.1"
                     /translation="MINSFEDFPLDPSLQRAVRAVGYETPTPIQSQAIMPLLEGNDLL
                     GIAQTGTGKTAAFALPILQLLLKSGKIRAPKCARALVLLPTRELAIQVSECFEQYAKY
                     TSISTTCVFGGVSDVSQKKALIRGVDVLVATPGRLLDLIGQKVVSLRALEFFVLDEAD
                     RMLDMGFIHDIRKIITMLPAVRQNLFFSATMPSDITKLASSILRKNPIRIEVVPESTP
                     IERIEQSVYKIDKKRKGALLKALLLAHPEMSKVLIFSRTKHGANRITETLEKAGITSA
                     AIHGNKSQTRRQEALGNFKCEQIRVLVATDIAARGIDVDGVTHVFNFDLPEVPETYVH
                     RIGRTARAGKGGVAIAFCAPDEESELRAIEKLTRQKIQEGDSSVLKELPVQKESSAHH
                     SPNANRRKPKRDSFKKKSPPVSSSESVQKSNPIKTKGRNRPGSRARKRLRATQVEQ"
     gene            complement(2241..2329)
                     /locus_tag="PLT31_00905"
     tRNA            complement(2241..2329)
                     /locus_tag="PLT31_00905"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2292..2294),aa:Ser,seq:gct)
     gene            2493..5684
                     /locus_tag="PLT31_00910"
     CDS             2493..5684
                     /locus_tag="PLT31_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HPW93723.1"
                     /translation="MRFFKKLGQIALAFFLVGLICLIPAYIVVFKILPLKDPDFLFNR
                     QSIVQALSGETRVFYNDGETHLGAFFDVNHRIYVPYGEIPEHVVNALVAAEDARFWEH
                     NGFDIRGFSRAMANNIRMMKLRQGGSTLTQQTVKNIFGREKTSVKEKWKELINALRME
                     KHFSKEDILEFYLNQFHVSGTGKGVAIAAQYFFNKDLRELSLAECAFIAGSVKGPFNY
                     DPFIQRNQARREKALERGRLRLKYVLDRMVEEKYISEAMRDSAIATPLHFEHGDFRFT
                     LSTTLTRLEEKLDSDFYREIFEKNQIEDWRKAQLQIITTLDAKYQDAATRALQNNISN
                     LQLQLGGFTLPKAELPNRAIKGRKGDYLYGAIDSVGYSEKGDLDFIHLSFGQLKGVID
                     SKTLKEFGKQVKGDPSKILASRLGKGSILLVSVLDSNLVNGYAPCKIETEPVLQGALI
                     AIQNGAVLATQGGFHNTGYDRSFKALRQLGSSWKPLLFGAALHYHWHYLDEIENSFNL
                     FQYVDQFYFPRPDHKKKGETVSILWAATRSENIASVWLLEHLLDKLSIEEYAEVAEKN
                     GYTVLPEEDEKAYYERLRDKFGLTLNNQVKQEIEFTKAKHLLVEKFLYEKQPRKARAL
                     KHLLYGRFVEKGLKEQRRSSENKALLKHNYLSYSEILRNRFAQELDPDLSLTLPPLDS
                     VKLFEDFSLADFKRLTVMIEPVDNEASYFEINQLKYWPDFNRSLSMAEFARFSNEIGI
                     QQKLKKVFSMSLGVNDISLAEMTTAYQTLLTGKAFKSLDGDWYEPCMIKEIKDRDGRL
                     LFKNEIESKTILNDTTTSQMGLMLRSVFEHGTARSQLRNLSVSSEDKHNTLLYPVVGK
                     TGTTNDYKNVAFIGGIPTFVNSKNGIALDSTIAVGSYVGFDDNRPLKSGRTRIAGASG
                     ALPQWAQFTKEVLDTRKESGKIDFLDITLLASKRVPILFTNERGMLPVNAETGLADAS
                     QSANVVEVPWLDVPGYIPPVVTTKAAEEAAEQGIFVSITMPLDTLKSKDSSKTTEPTP
                     VSQTTPTSTDWELPADFSGKDLFIPIEPELDLP"
     gene            5714..6862
                     /locus_tag="PLT31_00915"
     CDS             5714..6862
                     /locus_tag="PLT31_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93724.1"
                     /translation="MFYKFLFLSLIMSFFIACGSSAPVTEINPLVLKETEALVIKTPT
                     PDSIQKKDSVVKPDSLIIPIDTSTLSVDSIIEEKKLVVNPYRDIPALVQEVLKYADTL
                     LSKGKSDSAAAYLEQFIVLKPLWDEWQKEAQNIYLRTKKTQAMRAEEFKSLTLQIINM
                     NSVGASYDLIKTFTDSLVSLNPGDSLISWVEKQNKIAFQKNLSKARQEKEIILAEANK
                     SNQFKTAQEQITQLKARYYYFEDSLQLESTIAQLVEMENSISKQDELFWESNDPNKSL
                     EQAEKWIAEKKYSKARTLLQKLKSSILRQKAIEQMEVLAEAFCNEERKKTSVLFQSAQ
                     KTSNDTQKKKKLTQAIEGLNRCLDQYPEYSQKEKVINNRDFLENELSR"
     gene            6884..7636
                     /locus_tag="PLT31_00920"
     CDS             6884..7636
                     /locus_tag="PLT31_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPW93725.1"
                     /translation="MDYLQYLIFFSLGLIVSLINSIAGGGSSLSLPIMILLGLPPTVA
                     NGTNRIGLIVGNLSSMLNLKKHGYLYMPVFKQLFLPTLIGSFLGVVALVKMSDKLFQA
                     ILALVIFFVVFMTNLKKDILGKAPETPPSKRSLKGFLGFIAIGIYGSIIQVGVGFLQI
                     FAISKYSGLSLIQVNALKSALTSIFLVVSTIGLALSGKILWDVALIMALGAWLGGYIG
                     SSLQRKHGNQFIQRAITIVSLLMACYLLYDLF"
     gene            complement(7694..8356)
                     /locus_tag="PLT31_00925"
     CDS             complement(7694..8356)
                     /locus_tag="PLT31_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HPW93726.1"
                     /translation="MLKFTQEILLALRAISNEEEARCMSRNVREQFDFLGIKVVSRRE
                     VTYPIFDKFPPKDCEELVLRVDDMWGQPYREIQYAACDYLFRHKELLGGPHLNFLKKL
                     IKTRAWRDTVDTLATSVLGDLAWRMPALRTKIAAWIRDPNIWVRRSAIIFQLQYREKT
                     DWPLLKDFCSSCAKDDDFYVRNGIGRALSEYARINPSEVKRFVLSNTLPFQTSQEILK
                     NI"
     gene            8494..9114
                     /locus_tag="PLT31_00930"
     CDS             8494..9114
                     /locus_tag="PLT31_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93727.1"
                     /translation="MTTDDLGIIEVPPELRGLSDEEILKLNDVRAKKSRSSSRPSKKE
                     RLQKKQAKERERIKKIAEEYERKDFNPRQNTLMASKPMPKLSEFENPLEEKKSTLHKK
                     SDDLFKADFGSSSPSGLEKKNIEVKPLLSSTPSPSSGRVLKGVFGSKGKEPVKATIVK
                     AEELLKAKALERAKKLKTSIKVTPAVNETGEEPPKRPRGRPRKNPI"
     gene            9342..11318
                     /locus_tag="PLT31_00935"
     CDS             9342..11318
                     /locus_tag="PLT31_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HPW93728.1"
                     /translation="MRYGDHSFFQTGVAVPLFSLRSKNSIGIGEFLDLIPFANWAKYC
                     DLNVIQILPVNDTGNESSPYSARSAFALHPVYLNLQVIDGSSDFEEEIQKAKEEFNKE
                     LRIDFYKIVSWKRSILRKIFDSHFDKISSHKKLERWIDDNEWVKPYCVYALLKAKHQE
                     ASWKDWKTNQHPTAASIEKIWKNNFKEGLFQAWMQFEAEAQFKAAVKAISDLGVKLKG
                     DIPILINEDSADVWFHRNFFSLDDRAGAPPDMFSYSGQNWGFPTYRWDNIEKDNYSWW
                     RKRLFQASKFYHAYRIDHVLGFFRIWSIPQTEVTGALGQFNPSNPILRSDLEKKGFKK
                     ESLEYLRNPNFSVEQLLPFFKNHFSQSVLNEFFENLPNTTDRFIIKESFRSEKAILNS
                     SASQEVKDALLKVYWNRVFIPSGTEDHYYPFWYWYEQPVLFTLPESEQQLLKSIISEN
                     AASQEPLWQENALKLLNVLANETDMLVCAEDLGAIPHCVPFVLNKLNILSLRVERWSR
                     NWDVPYSPYYEMEDYPRLSVATTSCHDTSTLRGLWGESDFDKELFWQHAHQQGMPPEV
                     LYPNHVKGLIENIFSSNSLFCILPIQDYFGLSSQFSMISPELERINIPGTVGPHNWSY
                     RIPCTVEDLVKFSALNADIRSLVDARKRRPIWKI"
     gene            11315..12616
                     /locus_tag="PLT31_00940"
     CDS             11315..12616
                     /locus_tag="PLT31_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HPW93729.1"
                     /translation="MSFFKNTTPPSWVKEAIFYQIFPDRFAKSDRYHGPGQYDPWGTP
                     PTTQNICGGNLRGIEEHLDYIADLGCNAIYFCPLFKSNSNHRYHTYDYYKIDPILGTE
                     EDFDNLIKACHKKHIRVILDGVFNHCSRGFFEFNSLLEQGPESPYKDWFIVDSYPVCA
                     YDKTPNYRCWWNLPALPKFNTNNPEVREMLFSVAEYWTKKGIDGWRLDVPNEIDDDSF
                     WREFRTRVKKINSDAYIVGEIWDPPARWLQGDQFDGVMNYPLRRRLIDFLLRDFPKNN
                     EAFCDFLNACFPADSFGVSMNLIGSHDTRRIASQNHINSDILKLIWTILFFLPGCPNV
                     YYGDELALEGGQDPDNRRCFPWDNLKTIEKTSFSLFFKKLLKMRRSEIILQKGSFEAI
                     PCEEGFLLKRQLKKDFMVLRVFTPKIGGPDSFTLTKNDHNY"
     gene            12661..15312
                     /gene="secD"
                     /locus_tag="PLT31_00945"
     CDS             12661..15312
                     /gene="secD"
                     /locus_tag="PLT31_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HPW93730.1"
                     /translation="MNKKTFGLREMIILAILLLSCYTIWPSIQIHSKFGEEKKTFLKE
                     NPKLAAKSINFGLDLAGGTSITLEIDKTGLKDDDISDIQQQSLEIVRNRVDQYGLSEP
                     QIAPSGNSRIIVELAGVDDSTAKELVGSTAKLEFKILAEAEKFSQVVTLIDNYLSRQT
                     TDAAEEKAVASKDSTAVSDTTAAVAPAAEVAVSDEDLLKGIAPTTATTKAADSVKDTT
                     NKVIATNIPDATPLSSYYLSFGNGGFIGQDNIEKVKQILALETVQKLIPRDVVFAWGS
                     GLEKISKDSNIKAKRFYLLKRRAEMNGEDISDARPYRVGDGTNAGEVAVSLKFSGVGP
                     KKFSAVTAANVGKQMAIVLDDQVISAPVIRDRIPNGEAQITGLDDMAEANRLAVVLRA
                     GALKAPMNIIESRNIGATLGVENIKAGFGSGVIGLILCLIFMISYYRFGGFIASLAVI
                     CNLIISAAVLSVFNATLTLPGIAGFILVLGMALDANVIIYERIREEMKNGLLARAAVA
                     KGYEKAFSAIFDSNLTTFLTALILYKVGTGSVKGFGLTLMIGIATSLFCSLTITRAIL
                     DFKLSKRDSNKLSIGKGIRAINEVNLSIIPKSKLFISISLVFIAISLGSVLVKGFDFS
                     IDFMGGQVYTVQYDDNLRHEEDLTQILNTAGLSNAKVRHLGGTSANSYQISVKDLNDQ
                     SFKEQLSSLFEKNNQKAVIVASDTVGPTIGKELRFNAILSVLLSWIAICLYVWFRFGK
                     MGLGFGVGALVGLIHDTVITLGFISLAGLSVDGALIASLLTMIGYSVNDTIVVFDRVR
                     ENTGINGRAHFANTVNKSINECFSRTVITSITTLFVCTVIAFMGGSSIRDFGLVMVFG
                     IIVGTYSSIAVSSPFVVWWSKRFKGGL"
     gene            15385..16305
                     /locus_tag="PLT31_00950"
     CDS             15385..16305
                     /locus_tag="PLT31_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HPW93731.1"
                     /translation="MRKYYRIESGRIVPASDEESADIVMMSAPSPEQRGALIKEYELT
                     EHTIASAFDNDELSRIEYDDDFTTIVFKKPKNYAASDNFQFRVESFGIFLFNDWVLLL
                     TDSEIPLQDERRFSKIDSLNTVVLKLLNYSIFHFNEHLKIINRINDELEQKLRTAMEN
                     KYLLSMFSLNKGLIYYVSALNSNDTLLKKLQLGRSLNFSELERELLDDIIIENRQSLQ
                     QAEIYANILTSMMDARASVINNNVNSLMKTLNAITIALMVPTFVASIFGMNVEFPFSL
                     GVSNPYSFWIIMGIAILSVIIFMFFWQSKK"
     gene            complement(16319..17323)
                     /locus_tag="PLT31_00955"
     CDS             complement(16319..17323)
                     /locus_tag="PLT31_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPW93732.1"
                     /translation="MISSSSDFKDVLMSARGLVQEDLSLTEQVILGIAANAPQGISSR
                     MEQLVHRKGKRIRSTLLCLISHSGNTPPDLKRVSRACAGLELLHLASLVHDDIIDNTE
                     LRRGVRTAHKEWGNKIAVLIGDYILSQAMRCVIDEKDRTIPEILSNAANDLIGGEIME
                     LDDSGDMNLSYDRYIEIIHNKTASLVDAAARMGAIFAGFDSERVEACAKMGANFGLAF
                     QIIDDLLDYGIGAENLDKAKFTDIANGLVTLPVIFYFEACSPTEKEYMRTLICRASEP
                     KVPEEIISLLSKQGCFEKTKKVALDYLDSALDIAKALPESKFSEALELFFSAMSDRSN
                     "
     gene            complement(17327..19240)
                     /gene="gyrB"
                     /locus_tag="PLT31_00960"
     CDS             complement(17327..19240)
                     /gene="gyrB"
                     /locus_tag="PLT31_00960"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002379278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HPW93733.1"
                     /translation="MAEEYSGSSITVLEGLEAVRVRPAMYIGSTDGRGLHHLIWEVVD
                     NSVDEALAGYCTHIEVSILPGNGIRVTDNGRGIPTDMHPKEGIGTLEVVMTKLHAGGK
                     FDSNSYKVSAGLHGVGVSCVNALSEKLIASVRSKGRIIEQEFSKGLPLGPQKDVGPSD
                     GTTGTTITFYPDATIFTDTVFVYETLVTRFREMAFLMSGLTITVSDERNPENIITDSF
                     CYPGGVSAFVRFIDEHRKPLFENPIHISVDNGQFPLEVAMWYNESYQENFFSFVNNVN
                     THDGGTHVTGFKTALTRVITKFAQDLPKTKKDVQISADDIREGLTAVIAIKISQPQFE
                     GQTKRKLGNSEVAGYVNTMFGTKLEEFFHENPAVIKMILDKVYSAAVAREAAHRARNL
                     ARRKNVLESGGLPGKLADCSSRNPEECELFIVEGDSAGGSAKQGRTRDFQAILPLRGK
                     ILNVEKARLDRVLESEEIQNLINAIGCGVGTEFKIEKIRYHKIIIMTDADVDGSHIQT
                     LLLTFFFRYMRPLVEKGHVFLAQPPLYKVRVGKQDTYLFTDEDKDEMMKSLTEEKKNV
                     YLQRYKGLGEMNPEQLAETTMNPESRILRQCNIEDTVLADQVFSMLMGEEVEPRRRFI
                     ETNAYKVMDTLDV"
     gene            complement(19269..19583)
                     /locus_tag="PLT31_00965"
     CDS             complement(19269..19583)
                     /locus_tag="PLT31_00965"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HPW93734.1"
                     /translation="MMKRRRIKCFASKPTDISVLLERCLQGPVLGSKVGYFELSKHFD
                     EIVGERLVPYVSLVSLEDSILVLKVSNAVWKSELFLQKKAIIAKCNQFLNAAIVKEIR
                     FT"
     gene            complement(19586..>19958)
                     /locus_tag="PLT31_00970"
     CDS             complement(19586..>19958)
                     /locus_tag="PLT31_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW93735.1"
                     /translation="GLSPFKFLLAPRLVATVIMLPVLTIFSIFVGILGGFVVANLSKE
                     VSWHVFFYGVRMFYENWDFAVGLIKSVLYGYFIASYACFFGYTTHSGAEGVGKSTKAT
                     VVAGMTSILIGSFILSKLLLV"
BASE COUNT         6323 a         3960 c         3539 g         6136 t
ORIGIN      
        1 cacccctctg gaagatgccc ctcgaataga aatgctctga aaactaccta ttccgccttg
       61 tttatgtccc tgaataccac cgtaagaaga aaggatatca atcgctgtaa agttttttcc
      121 aacccacgct tcttcactaa tagatacgac ttgatttgaa agattttctg ttatagaagc
      181 accgatatgt acttctgttt cccctagatt ttgtaggggt aaagaatcgg tcaccgaagc
      241 cgaaacagag taaaataacc ccataaaaag ggctactagg ggtgatgtct ttcgcatcgt
      301 gcctctcgct attattctac agttcttctg actcggggat cactctactc ctagttcctt
      361 cacaccttta cggcattggt tttccggaca aaagtcctct tactagtttc gttctccctt
      421 acagcggcgg gaccgttccc gattttcacg ggattttctc aatcatttta cattcataaa
      481 acaattggct tttcaaggca tagaatatga tttaacgcaa atataataaa aaatgcttat
      541 tgttcaactt gagtagcacg caaacgtttt ctcgctctgc tacctggacg attacgccct
      601 tttgttttga tcggattact tttttgaaca gactcagaag aagaaactgg tggagatttc
      661 ttcttaaaag aatctctttt aggttttctt ctgtttgcat taggagaatg atgagcagaa
      721 gattcttttt gaacaggaag ctcttttaga acagaagaat cgccttcttg aattttttga
      781 cgagtgagct tttcgatggc tcttaattca gactcttcat cgggagcaca aaacgcaatc
      841 gccacgcccc ctttacctgc ccgagctgtt cgtccaatac gatgaacata cgtttctggg
      901 acttctggta aatcaaaatt aaaaacatgg gtcacaccat caacatcaat gcctcgagcc
      961 gcaatatctg tagcgactaa aacacgaatc tgctcgcact taaaattacc aagagcctct
     1021 tgtcgacgcg tttggctttt attaccatga atcgcagcac tagtaatccc tgctttttct
     1081 aaagtctccg taatgcgatt tgccccgtgt ttagtacggc taaaaatcaa cactttcgac
     1141 atttcagggt gggctaaaag taatgctttt aatagagccc cttttctttt tttatcaatt
     1201 ttataaacag attgttcgat tcgttcaatg ggagtacttt ctggcacaac ttcgatacga
     1261 ataggattct ttcgtaaaat tgaagaagcc aatttggtga tatcagaagg catcgtcgca
     1321 gagaaaaata agttttgacg aacagcagga agcatcgtaa tgatttttcg aatatcgtga
     1381 ataaatccca tatcgagcat acgatctgct tcatcaagca caaaaaattc taatgctctt
     1441 aaagaaacta ccttctgacc aattaaatcg agtaatcttc caggagttgc cactaaaaca
     1501 tctacgccac gaattaaggc ctttttttgc gatacatcgc tcacgccacc aaaaacacat
     1561 gtggtagaaa tagacgtata ttttgcatat tgttcaaagc actcagatac ttggattgca
     1621 agttcacgag taggcaataa aacaagcgct ctagcgcatt ttggagcacg tattttccct
     1681 gattttaaga gaagttgaag aattggcaat gcaaaagccg ctgttttccc tgttcctgtt
     1741 tgggcaattc ccaaaagatc gtttccttca agaagaggca taatggcctg agattgaatt
     1801 ggagtgggag tttcatagcc aacggcacgg actgctcgct gtaaagacgg gtcaagagga
     1861 aaatcctcaa aagaattaat catatgaaca caaattagaa tttaataggc tgattccaaa
     1921 ccctgcaatg agaaaaacag ctattcttat attcacaaca cctaaagttc ttttcgctcg
     1981 ttttatgaca attctgtcgt ttttcaggca ttctgctaac ttatttgcaa tatttgtgaa
     2041 atatactatg gctctttttg attaaacgca ataagccaaa ttgaatcaaa ccccacaaaa
     2101 ccgccatttt tttgaatatg aatatcaaga aaaaagacga atgattttgt tgaaaaatgg
     2161 agtttatctg ataataggct tttaatagac tttttaaaaa caaaaaagct tctataccta
     2221 agtatagaag ctttttgaag cgggaagagc gagattcgaa ctcgcgatag gattaagtcc
     2281 tatacgtcct tagcagggac gcgccttagg ccagctcggc catcttcccg tttatctcgc
     2341 acaattctaa ataaaaatgt tctctttttc aagcgaacat cattaaaaaa atacgaatac
     2401 atcaaaatat gagcctaaaa cttgtctttt tctaataaaa gaggtgtttt gtttttaatt
     2461 tttacaaaat ttttattcgt tgagactatg gcatgagatt ttttaagaaa ttagggcaaa
     2521 tagccttggc ctttttttta gtaggactga tctgtcttat tcccgcttat attgtggttt
     2581 ttaagattct tcctttaaaa gaccctgatt tcttatttaa tcgtcaatcg attgtacaag
     2641 ctctttctgg agaaactcgc gttttttata acgatggcga aacccatcta ggtgcatttt
     2701 ttgatgtcaa ccatcgcatt tatgtcccct atggagaaat tcctgaacac gtagtgaacg
     2761 ccctcgttgc tgctgaagac gcccgttttt gggaacataa tgggtttgat atccgtggtt
     2821 tttcaagagc catggctaat aacattcgta tgatgaaatt gcgccaaggc ggttcaacac
     2881 tcacccaaca aacagtcaaa aatatttttg gtcgtgaaaa aacttctgtt aaagaaaagt
     2941 ggaaagagtt gattaacgcc cttcgtatgg aaaaacattt ttcaaaagaa gatattcttg
     3001 aattttatct aaaccaattt catgtttctg gaactggtaa aggggtggcc attgccgctc
     3061 aatatttttt taataaagac cttcgagagc tttcgcttgc agagtgtgct tttatcgctg
     3121 gttcagtcaa aggtcctttt aattacgatc cctttattca aaggaatcaa gcccgtcgag
     3181 agaaagctct agaacgtgga cgattgagat taaaatatgt tttagaccga atggttgagg
     3241 aaaaatacat ttcagaagcc atgagggatt ctgccatcgc tactcccctt cattttgagc
     3301 acggtgattt tcgttttacg ttgtcaacca cactcacaag actcgaagaa aaactggact
     3361 ctgattttta cagagaaatt tttgagaaaa atcaaattga agattggaga aaagcacaac
     3421 ttcaaatcat cactaccctt gatgcgaaat accaagatgc cgccactcga gctcttcaaa
     3481 acaatatatc gaacctccaa cttcagttag gtggctttac tttacctaaa gcagaactcc
     3541 ctaatagagc gatcaaaggc agaaaaggag actacctata tggagctatt gattctgttg
     3601 gttattctga aaaaggagat ctagacttta ttcatctctc ttttggtcag ctaaaaggag
     3661 taattgattc taaaacgctt aaagaatttg gaaagcaagt caaaggcgac ccatctaaaa
     3721 ttcttgcttc gcgtttaggg aaagggtcta ttctacttgt cagtgtttta gatagtaatt
     3781 tggtcaatgg atatgcacca tgtaaaattg aaactgaacc cgtgctccaa ggtgcattga
     3841 tcgcgattca aaatggagcc gttcttgcca ctcaaggtgg ttttcataat acaggctatg
     3901 atcgttcttt taaggccctt cggcaacttg gttctagctg gaagcccctt ctttttggcg
     3961 ccgctctaca ttatcattgg cattacttag atgaaattga aaattcgttt aacttatttc
     4021 aatatgtaga tcaattttat tttcctcggc cagatcacaa aaagaaaggc gaaaccgtga
     4081 gcattctctg ggctgctaca cgctctgaaa acattgctag tgtgtggctt ttagaacatc
     4141 ttttagataa actctctatt gaagaatatg ccgaagtagc agaaaaaaat ggttatacag
     4201 ttcttccaga agaagatgaa aaagcctact atgaacgtct ccgagataag tttggtctca
     4261 ccttaaacaa tcaggtcaaa caagaaatcg aatttactaa agcaaaacat cttttagtag
     4321 aaaaatttct ttacgaaaaa caacctcgta aagcacgtgc tctaaagcat ctattatatg
     4381 gtcgttttgt ggaaaaaggt ttaaaagagc aacgccgctc ttcagaaaac aaagcgttat
     4441 taaaacataa ttatttaagt tattctgaga tattaagaaa tcgttttgca caagaactcg
     4501 accctgattt atcgctaacc ttaccgcctc ttgattccgt taaattattt gaagattttt
     4561 ctcttgctga ctttaagcga ttaacggtaa tgattgaacc cgttgataat gaagcctctt
     4621 attttgaaat aaatcaatta aaatactggc ctgattttaa tcgttcgtta tccatggctg
     4681 aatttgctcg tttttctaat gaaataggga ttcagcaaaa gctcaaaaaa gtatttagta
     4741 tgtctcttgg agtaaacgat atttccttag ctgaaatgac aaccgcctat caaacactcc
     4801 tcacaggaaa agcatttaaa tctctcgatg gggattggta tgaaccatgt atgattaaag
     4861 aaattaaaga tcgtgatgga cgtcttttgt ttaaaaatga gattgaatcc aaaacaatct
     4921 taaacgatac aaccacttct cagatggggc taatgcttcg ctccgtattt gaacacggta
     4981 ctgcaagaag tcagcttcga aacttaagcg tttcttctga agacaaacat aatactcttc
     5041 tttaccctgt tgttggaaaa actggaacca caaacgatta taaaaacgtc gcctttattg
     5101 gaggcattcc tacttttgta aattcaaaaa acggaatcgc acttgattcc actattgctg
     5161 tgggaagtta tgttggcttt gacgacaacc gtcctctaaa atcaggaaga acacgtattg
     5221 caggtgcttc aggagccctt cctcagtggg cccaatttac aaaagaagta ttagacacta
     5281 gaaaagaaag tggaaaaata gactttttag acattaccct tttagcctcc aaaagagtcc
     5341 ctattctctt taccaacgaa cgcggaatgc ttccagttaa tgcagaaaca ggcttggctg
     5401 atgcatctca atcggcaaat gttgttgaag ttccttggct tgatgttcct ggctacattc
     5461 ctcctgtcgt caccacaaag gctgccgaag aagcagcgga acaaggaatt tttgtttcca
     5521 ttacaatgcc tttagacaca ttaaaaagta aagactcttc taaaacgact gaaccaactc
     5581 ctgttagtca aaccacaccc acttctactg attgggaatt acctgctgac tttagcggta
     5641 aagatctttt cattcccatt gaaccagaat tagatttacc ttaaggataa agacttatat
     5701 cgaggatcac tacatgtttt ataaatttct tttcttgtct ttaatcatgt ctttttttat
     5761 cgcctgtgga tcaagtgctc ctgttacaga aatcaatcca ctagtattaa aagaaactga
     5821 agctctagtg atcaaaacac caactccaga ctcaatccaa aaaaaggatt ctgttgtaaa
     5881 gccagattct ttaattattc ctatagacac atctaccctt tcagttgatt ctattatcga
     5941 agaaaaaaaa ctggtggtaa atccataccg tgacattcct gctcttgtac aagaagtttt
     6001 aaaatatgca gacacccttc tttcaaaagg taaatctgat agcgccgccg cttacttaga
     6061 acaatttatt gttctaaagc ctctctggga tgaatggcag aaagaagcac aaaatatata
     6121 cttacgtaca aaaaagactc aagcgatgcg agcggaagaa ttcaagtctc tcactcttca
     6181 gattataaat atgaattccg ttggcgcaag ctatgattta atcaaaacat ttaccgatag
     6241 cttagtttca ttaaatcctg gtgactctct tatttcttgg gtagaaaagc aaaataaaat
     6301 cgcttttcaa aaaaacttaa gtaaagcacg gcaagaaaaa gaaataattc tagctgaagc
     6361 caataaaagt aatcagttta aaacagctca agaacagatc acacaattaa aagcccgcta
     6421 ttattatttt gaagactcct tacaattaga atccactata gctcaattag tagaaatgga
     6481 aaattctatt tctaaacaag atgagctttt ctgggaatct aatgatccta ataaatcttt
     6541 agaacaagct gaaaaatgga ttgctgaaaa aaaatattca aaagcaagaa cattattaca
     6601 aaaacttaaa tctagtattc ttcgtcaaaa agctatagaa caaatggaag tattagcaga
     6661 ggctttttgt aatgaagaac gaaagaaaac gtctgtttta tttcaatccg cacaaaaaac
     6721 ttctaacgat actcaaaaaa agaaaaagct gactcaagcg attgaaggat taaatcgatg
     6781 cttagatcaa taccctgagt atagccaaaa agaaaaagtc attaataatc gtgacttttt
     6841 agaaaacgaa ttgtctcgat aagaaaatca aggctttcat aaaatggatt acttacaata
     6901 ccttatattc ttttctttag gcttaatcgt aagccttatt aatagtatcg ctggaggagg
     6961 tagctcatta agtttaccta ttatgattct tttaggtctt cctccaacag ttgctaatgg
     7021 aacaaatcgg attggtctta ttgttggcaa tttaagttcg atgctcaatc taaagaaaca
     7081 tggctattta tatatgcctg ttttcaagca gcttttttta cctactttaa taggttcttt
     7141 tttaggagtt gttgctcttg taaaaatgag cgataaatta tttcaggcta ttcttgccct
     7201 cgttattttt ttcgttgtct ttatgacaaa tttaaagaaa gacattctag gcaaagcacc
     7261 tgaaacacct ccttcaaaaa gaagcctcaa aggtttttta ggttttattg cgattggtat
     7321 ttacggaagt attattcaag ttggcgtagg ctttctacaa atctttgcta tttcaaaata
     7381 ctctggctta agcttaattc aagtaaatgc attaaaaagt gctctgactt ctattttctt
     7441 agttgtaagc actatcggac tagccctctc tggaaaaatt ctttgggatg tagctctcat
     7501 tatggctctc ggtgcttggc tcggcggtta tataggcagc tctcttcaaa gaaaacacgg
     7561 taatcagttt atccaaagag caatcacgat agtgagtctt ttaatggcct gctatttatt
     7621 gtacgattta ttttgataaa aaaactctcc aaagaaagga gagttctata atcatccaaa
     7681 cattataatc attttaaatg ttttttaaaa tttcttgaga cgtttggaaa ggaagagtat
     7741 tacttaacac aaaacgtttt acttcagaag gattaatcct tgcatattcg ctcaaggcac
     7801 gaccaattcc atttcgaaca taaaagtcat catcttttgc acaagaggaa caaaaatcct
     7861 ttagtaaagg ccaatccgtt ttctctcgat attgtaattg gaaaatgatt gcactacgac
     7921 gaacccagat attaggatcg cgaatccaag cggcaatttt tgttctaaga gcaggcatgc
     7981 gccaagctaa atcacctaag acacttgtag ccaaagtatc tactgtatct ctccaagcgc
     8041 gagtctttat taattttttt aagaaattca aatgtgggcc accaagaagt tctttatgcc
     8101 gaaataaata atcgcaagcc gcatattgaa tttcacggta aggttgcccc cacatatcat
     8161 ccactcgaag aacgagctct tcacaatctt taggcggaaa cttgtcaaat ataggatatg
     8221 tgacctctcg cctagatacc acctttatac ccagaaaatc aaattgttct ctgacgtttc
     8281 tggacatgca acgagcttcc tcttcatttg agatagctct taaagccagc aatatttctt
     8341 gagtaaattt aagcatcttt aacaaaaata acccagagac acattatttt ctaggccttg
     8401 acaaacataa attcaatttt tttttacctt ttagcaacat tttaaagctt tttattcaaa
     8461 aaaaacacct ttttaggtaa aaaaatcaaa tttatgacaa ctgatgattt aggtattata
     8521 gaggttcctc ctgaattgag aggactttct gacgaagaaa ttctgaaatt aaatgacgtt
     8581 cgggccaaaa aaagtcgttc cagcagccgc ccctctaaaa aagagcgctt acaaaaaaag
     8641 caagccaaag agcgagaacg aattaaaaag attgcggaag agtacgaacg aaaagatttc
     8701 aatcctcgtc aaaacacact aatggcttca aagcctatgc ctaaattaag cgagtttgaa
     8761 aatcctttag aagaaaagaa atcaactctt cataaaaaat cagacgacct atttaaggcg
     8821 gattttggat catcctcacc ctctggtcta gaaaaaaaga atattgaagt aaaacctctt
     8881 ttatcctcaa cccctagtcc tagttcaggt cgagttctaa aaggagtctt tggttctaag
     8941 ggtaaagagc cagttaaagc aaccattgta aaagccgaag agctcttaaa agcaaaagct
     9001 ttagaacgag caaaaaaatt aaaaacatca ataaaagtca ctcctgctgt taatgaaaca
     9061 ggagaggaac cacctaaacg tccaagagga cgtcctagga aaaatcctat ttgatattct
     9121 ttttattctg ttttttctga gccagagtct ctttttttga gtcttgcttt tcttttaacc
     9181 caaaaacaaa ccgtcctcgt aagacgcacc caggagatcc tggatcgcat tcttttagcc
     9241 ttttgtggca aaaagtctcc tctaaaaacc gacattcata actcataaag acacctttat
     9301 ataatgttta gcattcttaa aaaataactt atctttaagt tatgagatac ggtgatcatt
     9361 cattctttca aacaggtgta gcagttccac tatttagctt acgcagtaaa aatagtatag
     9421 ggattggcga atttctggac ttaattcctt ttgctaattg ggcaaaatat tgtgacctta
     9481 atgtcattca gattctacct gtaaacgata caggaaatga atccagcccc tacagtgcaa
     9541 gaagtgcatt tgctcttcat cctgtatatc ttaatcttca agtaatagat ggatcttccg
     9601 atttcgaaga agaaatacaa aaagcaaaag aagaatttaa taaggaactt cgaattgatt
     9661 tttataaaat cgtctcttgg aagcgatcca tattaagaaa aatttttgat tctcatttcg
     9721 acaaaatttc ttctcataaa aaactcgagc gttggattga tgacaatgaa tgggtaaagc
     9781 catactgtgt ttatgcttta ctcaaagcaa agcatcaaga agctagttgg aaggattgga
     9841 aaacaaatca acatcctact gctgcaagca ttgaaaaaat ttggaaaaac aatttcaaag
     9901 aaggactctt tcaagcttgg atgcaatttg aagcagaagc tcaatttaaa gcagccgtaa
     9961 aagcaatctc tgatttaggt gtaaaattaa aaggcgatat tcctatatta attaatgaag
    10021 acagcgctga tgtttggttt catagaaatt tcttttctct tgatgatcgc gctggagctc
    10081 ctcccgatat gtttagttac agcggccaga actgggggtt cccaacctac cgctgggata
    10141 atattgaaaa agataattac tcttggtggc ggaaacgctt atttcaagca agtaaatttt
    10201 atcacgctta tcgcatcgat catgttcttg gcttctttag aatctggagt attcctcaaa
    10261 cagaagtgac aggagcatta ggccaattta acccttcaaa tcctatatta agaagcgatc
    10321 tcgagaagaa gggttttaaa aaagaatctc tagaatattt aagaaatccc aatttttctg
    10381 tcgaacaact actaccgttt ttcaaaaatc atttttcaca atccgtttta aatgagttct
    10441 ttgaaaacct acctaatacc actgatcgct ttattataaa agaatcattc cgttcggaaa
    10501 aggccattct taatagttcg gcaagtcaag aagtaaaaga tgcgttactc aaagtctatt
    10561 ggaatcgagt ttttattcct tctggtacgg aagatcatta ttatcctttc tggtattggt
    10621 acgaacagcc tgttctgttt actcttccag aatcagaaca acaattactt aaaagcatta
    10681 ttagtgaaaa cgccgcttca caggaacctt tgtggcaaga aaacgctctt aaattattaa
    10741 acgttctcgc caatgaaaca gacatgttag tatgtgcgga agatttaggt gcaattcctc
    10801 actgcgttcc ttttgtactt aataaactca acatattatc tttacgtgta gaacgctgga
    10861 gtcgtaactg ggacgttcct tattctcctt attatgaaat ggaagattac cctaggctct
    10921 ccgttgctac cacaagctgc catgacacat cgactctacg aggcctttgg ggcgaatccg
    10981 attttgataa agaattattc tggcaacacg ctcatcaaca aggcatgcca ccagaagttc
    11041 tttatcccaa tcatgtaaaa ggattaatcg aaaatatttt ttcttcaaat agtttattct
    11101 gcattcttcc tatacaggat tattttggat tgtcctctca gttttcaatg atttctccag
    11161 aacttgaacg aatcaatatt cctggaactg tagggcctca caattggtct tatagaatac
    11221 catgtactgt tgaagatttg gtgaaatttt ctgctttaaa tgccgatatc agatcactcg
    11281 tggatgcacg caaacgtcgt cctatatgga aaatatgagt ttttttaaaa atactactcc
    11341 cccatcttgg gttaaagagg ccatttttta tcaaattttt ccagatcgtt ttgcaaaatc
    11401 cgatcgctac catggacctg gacaatatga tccatggggt actcctccaa caacacaaaa
    11461 catctgtggt ggtaatcttc gtggtataga agaacatctt gattacattg ctgatcttgg
    11521 ttgtaatgct atttattttt gtcctttatt taaaagtaat tccaatcatc gttatcatac
    11581 ctacgattat tataagatag atccgattct tggcaccgaa gaagactttg acaatctcat
    11641 taaagcctgc cataagaagc acatccgagt gattctcgat ggtgtattca atcactgttc
    11701 tcgtggtttt tttgaattca atagtttgtt agaacaaggc cccgagtcgc cttataaaga
    11761 ctggtttata gtagactctt atccagtttg tgcttatgat aaaacgccta attaccgctg
    11821 ttggtggaac ctaccagcac ttcctaaatt caatacaaat aatccagagg ttcgagaaat
    11881 gcttttttct gttgcagaat actggactaa gaaagggatt gatggctggc gtttagatgt
    11941 tccaaatgaa attgatgacg attccttctg gcgtgaattt cgaacccgcg tcaaaaaaat
    12001 aaattcagat gcttatattg tcggagaaat ttgggatccc cctgctaggt ggcttcaagg
    12061 ggatcaattt gatggagtga tgaattatcc attaagacgc cgacttattg atttcttgtt
    12121 gagagatttt cccaaaaaca acgaagcttt ttgtgacttt ttaaatgcat gctttccggc
    12181 agactcattt ggggtatcca tgaatctaat aggaagtcac gatactagac gcattgcatc
    12241 ccaaaatcat atcaattcag atattttaaa attaatctgg actattctct tcttccttcc
    12301 tggctgccca aacgtatact acggagatga gctcgcttta gaaggcggac aagaccccga
    12361 taacaggcgt tgttttccat gggacaatct taaaaccata gaaaaaacat ccttttctct
    12421 atttttcaaa aaattattga aaatgagacg aagcgaaata atactccaaa aaggttcctt
    12481 cgaggccatt ccatgcgaag agggctttct cttaaagaga caattgaaga aagattttat
    12541 ggttctacgc gtatttacac ctaaaattgg tggtcctgat tcttttacgc ttaccaaaaa
    12601 cgatcataat tattaaattt gacgccgact tctagcatta atgcctctaa gggattacta
    12661 atgaataaaa aaactttcgg tttgcgagaa atgattattc tcgctattct actcctctct
    12721 tgttacacaa tctggccttc tatccagatt cactccaaat ttggtgaaga aaagaaaact
    12781 tttctaaaag aaaatccaaa gcttgcagcg aaatccatca atttcggatt agatcttgct
    12841 ggtggaacaa gtattactct tgaaatagat aagactggtc tcaaagacga tgatattagc
    12901 gatatccaac aacaatcgct tgaaatcgtc cgaaatcgtg tagaccaata tggcttgtct
    12961 gaaccacaaa ttgctcctag tggtaatagt cgaatcattg tcgaactggc aggtgtcgat
    13021 gattctaccg ctaaagaatt agtgggaagc acagctaagc tcgaatttaa gattctcgct
    13081 gaagcagaaa aattcagtca agtagtcacc cttatcgata actatctctc tcgccaaact
    13141 actgacgcag cagaagaaaa agcagttgct tctaaagatt caacagccgt tagtgatact
    13201 accgcagcgg ttgcaccagc agcagaggtt gccgtttctg atgaagactt gttaaagggc
    13261 attgctccaa caaccgctac cacaaaagca gctgattctg ttaaagacac tacaaacaaa
    13321 gtaattgcaa ctaacatacc tgatgcaact cctctttcta gttactactt gtcttttggt
    13381 aatggtggtt ttattggtca agacaacatt gaaaaggtaa aacaaatatt agcacttgaa
    13441 accgttcaaa agctaatccc acgtgacgtc gtttttgctt ggggtagcgg tcttgaaaaa
    13501 atttccaaag actcaaatat taaggcaaaa cgtttttatc tattaaaacg ccgtgctgaa
    13561 atgaatggtg aagacatttc agatgctcgt ccttaccgtg taggtgatgg aacgaacgcg
    13621 ggtgaagttg ctgttagctt aaaattcagt ggtgtaggtc ctaaaaaatt ctcagccgtc
    13681 actgctgcaa acgtcggcaa gcaaatggct atcgttcttg atgatcaagt catcagtgct
    13741 ccagtgattc gtgatcgtat tccaaatggt gaagctcaaa ttactggctt agatgatatg
    13801 gcagaagcta accgtcttgc ggttgtctta cgtgctggtg ctcttaaagc tcctatgaat
    13861 attattgaat ccagaaacat tggagctact ttaggtgttg aaaacattaa agcaggcttt
    13921 ggatcaggcg ttatcggctt aattctttgc cttatcttta tgatttctta ttatcgattc
    13981 ggcggcttca ttgcgtcatt agccgtgatc tgtaacttaa tcattagtgc tgcagttctc
    14041 tctgtattta acgccacttt aactcttcca ggtattgctg gctttattct tgtgctaggt
    14101 atggctcttg atgccaacgt gattatttat gaacgtattc gcgaggaaat gaaaaacggc
    14161 cttttggctc gtgctgctgt tgcaaaaggt tacgaaaaag ctttcagtgc tatttttgac
    14221 tccaacttaa ccacatttct tacagcgctc attctttata aagtagggac aggatctgtt
    14281 aaaggttttg gtcttacatt aatgattggt atagccactt ccttgttctg ctctcttacc
    14341 attacacgtg ctattcttga ctttaagctt tcaaagcgtg attcgaacaa gttgagtatt
    14401 ggtaaaggca ttcgtgctat taatgaagta aacctttcta tcattcctaa gagcaaactt
    14461 tttattagta tttctcttgt tttcattgca attagcctag gttcagttct cgttaaaggg
    14521 ttcgatttta gcatcgactt tatgggtggt caagtttata ctgttcaata cgatgataat
    14581 cttagacatg aagaggactt aactcaaata ctaaataccg cagggctaag taatgcaaaa
    14641 gtgcgccatt tgggaggcac atcagcaaat tcttaccaaa tcagcgttaa agatttaaat
    14701 gaccaatcat ttaaggaaca gctatcttca ttattcgaaa agaacaacca aaaagcagtt
    14761 attgttgcaa gtgataccgt tggccctacc attggaaagg aacttcgttt taatgccatt
    14821 ctttctgttt tattatcttg gatagccatc tgtctttatg tctggttccg ttttggaaaa
    14881 atgggtcttg gctttggtgt aggtgctctt gttggtctta ttcatgacac agtcattact
    14941 cttggtttta tttctttagc aggactatca gtagacggtg ctttaattgc gtcccttctt
    15001 acgatgattg gttactcagt aaacgatacg atcgtggtat ttgaccgtgt tcgcgaaaat
    15061 acgggtataa atggacgagc tcattttgca aatactgtaa ataaatctat taatgagtgt
    15121 tttagtcgta cagtaattac ctctataaca acactttttg tttgtactgt tatagctttt
    15181 atgggcggtt cttccattcg tgactttggt ttagtgatgg tcttcggcat tattgtagga
    15241 acatactctt ctatagcagt ttcatcacca ttcgtggtat ggtggtcgaa acgcttcaag
    15301 ggtggcctat aaaaatcaat cctaaaagcc tggggttgcc ccaggctttt tttatcctat
    15361 attagaacga gcaaaggtgg ttttatgcgc aaatactaca gaatcgaatc cggtcgtata
    15421 gttcccgcat cagatgaaga atcagccgat atagtcatga tgagtgctcc aagccctgag
    15481 cagcgaggag ctctcattaa agagtatgaa ctcactgagc ataccattgc ttctgctttt
    15541 gataatgatg aactttcgcg tatagaatat gatgatgatt ttaccaccat tgtgtttaaa
    15601 aagccaaaga attacgccgc ttcagataat ttccaattta gagtagagtc ttttggaatt
    15661 tttctattca atgactgggt tctcttatta accgattcag agattcctct tcaagatgaa
    15721 cgtcgcttct ctaaaataga tagtctaaac acagtcgtat taaaactatt aaactactct
    15781 atattccatt ttaatgaaca tttgaaaatc attaatagaa tcaatgacga gttagaacaa
    15841 aagcttagaa ccgccatgga aaacaaatac ttgttatcca tgtttagctt aaacaaaggt
    15901 cttatctatt acgttagtgc attaaattca aacgacacgt tacttaagaa acttcaactt
    15961 ggacgtagcc ttaatttttc tgaattagag cgagaactat tagatgatat catcatcgaa
    16021 aacaggcaga gtttacaaca agctgaaatc tatgcaaata ttttgactag tatgatggac
    16081 gccagagcaa gtgttattaa taacaacgtg aactcattaa tgaagacatt gaacgccatc
    16141 accattgctc ttatggtgcc tacgtttgta gcaagtattt tcggcatgaa cgttgaattc
    16201 ccattttcct taggcgtatc taatccttat tcattttgga ttattatggg gattgccatt
    16261 ttgtctgtta ttatttttat gttcttctgg caatcaaaaa aatagatggc gtctattttt
    16321 aattagatcg atcactcatt gcgctaaaaa acaattccaa agcttctgag aactttgatt
    16381 caggcaatgc tttagcaata tctaatgctg aatctaaata gtctaaagcc acctttttcg
    16441 ttttctcaaa acaaccctgc tttgataaga gcgaaataat ttcttctgga acttttggtt
    16501 ctgaagcacg acaaatgagt gttctcatat attctttttc agttggtgaa caagcttcga
    16561 aatagaatat cacaggaagc gtaactaagc catttgcaat atctgtgaat ttggccttat
    16621 ctaaattttc ggcaccaata ccataatcga gtaagtcatc aataatttgg aaggctaatc
    16681 caaaattagc ccccattttc gcacatgctt ctactctttc agaatcaaag ccagcaaaaa
    16741 tagcacccat tcgggcagcc gcatccacaa gagaagctgt tttattatga attatttcga
    16801 tataacgatc atagctcaaa ttcatatcgc cagaatcgtc taactccata atttcaccac
    16861 caatcaaatc atttgcagca ttagatagta tttctggaat agtacgatct ttttcatcaa
    16921 tcacacaacg catcgcctga gaaaggatat aatcaccaat cagaaccgca atcttgttac
    16981 cccattcttt atgggcagta cgaacaccgc gacgaagctc tgtattatca ataatgtcat
    17041 catggacaag acttgctaag tgaagtaatt caaggcctgc acaagcccgt gacacccttt
    17101 ttaaatctgg tggagtattc ccagaatgag aaatcaagca aagaagtgta gacctgatac
    17161 gctttccttt gcgatgaact aactgctcca tacgagagct aattccctgt ggtgcatttg
    17221 ctgcaatacc caagatcacc tgttccgtta gagacaagtc ttcttggact aaaccacgag
    17281 cagacattaa aacgtcttta aaatcacttg aagaagaaat cataaatcaa acatctaagg
    17341 tgtccatcac tttatatgca ttcgtttcta taaagcgacg acgaggttca acttcttcac
    17401 ccattaacat actaaacact tgatcggcaa gaacggtatc ttcaatatta cattgacgta
    17461 aaatacggct ttctggattc atcgtagttt ctgcaagctg ctctgggttc atttcaccaa
    17521 gacctttata tcgttgcaaa taaacattct tcttttcttc tgttaatgac ttcatcattt
    17581 catctttatc ttcgtctgta aacaagtaag tatcttgctt tccaacacga actttatata
    17641 aaggaggttg agctagaaag acgtggcctt tttcaacaag agggcgcata taacggaaga
    17701 agaacgtcaa tagaagcgtt tgaatatgag aaccatcaac atcagcatca gtcataataa
    17761 taattttatg atagcgaatt ttctctatct taaattcggt accaacaccg caaccaatgg
    17821 cgttaattaa gttctgaatt tcttcgcttt caaggactcg atccaaacga gccttttcca
    17881 cgttcaaaat ttttccacgt aatggtaaaa tggcctgaaa atcacgggta cgcccttgct
    17941 tagcagaacc accagcagag tcaccttcca caataaagag ttcgcactct tctggattac
    18001 ggctactaca atctgcaagc tttccaggta atccaccact ttcaagaacg ttctttcgac
    18061 gagctaaatt tctagctcgg tgagctgctt cgcgagcaac agccgccgaa taaaccttat
    18121 ccagaatcat tttgataaca gctggatttt catggaaaaa ttcttctaac ttagtaccga
    18181 acatggtatt gacatacccg gcaacctcag agtttcctaa tttccgtttt gtctgacctt
    18241 caaattgtgg ctgagaaatc ttaatagcga tcacagcagt aagaccttcg cgaatatcat
    18301 ctgcactgat ttgaacatct ttctttgtct ttggaagatc ttgtgcaaat ttggtaataa
    18361 ctcttgttaa ggctgtttta aaacctgtca catgcgtacc accatcatgt gtattcacgt
    18421 tatttacaaa actaaaaaag ttttcttgat aactttcatt ataccacatg gccacttcaa
    18481 gagggaattg accattatca acggaaatat gaattggatt ttcaaacaaa ggcttacgat
    18541 gttcatctat aaaacgaaca aaagcagaaa caccaccagg gtagcagaaa gaatccgtaa
    18601 taatattttc tggattacgt tcatcagaaa cagtaatcgt taagccgctc attaagaaag
    18661 ccatttcacg gaatcgcgtg acaagcgttt cataaacaaa aacggtatca gtaaaaatag
    18721 tggcatctgg ataaaaggtg attgttgtac cagttgttcc atccgaagga cctacatctt
    18781 tctgaggacc taagggcaag ccttttgaaa actcttgttc aataatgcgg cctttgcttc
    18841 gaacactggc gattaatttt tcagatagag cattaacaca actaacaccc acaccatgca
    18901 aacccgcaga aactttataa gaattactgt caaactttcc accagcatgc aacttagtca
    18961 tcaccacttc aagagttccg atgccttctt ttggatgcat atccgtagga ataccgcgtc
    19021 cattatcagt aactcgaatt ccattcccag gcaaaataga aacttcaata tgagtacaat
    19081 agccagcaag agcttcatcg acagagttgt ctacaacttc ccagatcaaa tgatgtaaac
    19141 cgcgtccatc ggtagatccg atgtacattg caggcctaac gcgtactgct tcgagtcctt
    19201 caagaacggt aatactcgat ccactatatt cttcagccat atttcctcaa cgtcaaaaaa
    19261 aactattact atgtaaagcg aatttccttt acaatagctg cattcaaaaa ctgattacac
    19321 ttagcaataa tagctttttt ttgcaaaaat agctctgatt tccacacagc attgcttact
    19381 tttagcacta aaatagagtc ttctaaactc actagagaaa cataaggaac aaggcgttct
    19441 ccgacaattt catcaaagtg ttttgatagt tcaaaataac ccactttaga gcctaaaaca
    19501 ggaccttgta agcatctttc tagtaatacc gagatatctg taggcttaga tgcaaagcac
    19561 tttatacgcc gtcttttcat catttctaaa ccaaaagaag ttttgaaaga ataaagcttc
    19621 ctatcaaaat acttgtcatc cctgcaacga cagtggcttt tgtacttttt cctacacctt
    19681 cagctccact atgagtggta taaccaaaga aacaagcata gcttgcaatg aaatagccgt
    19741 ataaaacaga cttaattaaa ccaacggcaa aatcccagtt ttcataaaac atccggacgc
    19801 cataaaagaa gacatgccat gatacttctt ttgaaagatt agcaactaca aaaccaccta
    19861 aaattccaac aaaaatacta aagattgtta gtacaggaag cataatcaca gtagctacta
    19921 aacgaggtgc gagtaaaaac ttaaatggac tcaagcct
//
LOCUS       DAOOBB010000009        19652 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_3674_length_19652_cov_4.448487, whole genome shotgun sequence.
ACCESSION   DAOOBB010000009 DAOOBB010000000
VERSION     DAOOBB010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 19652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19652
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3674_length_19652_cov_4.448487"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1220
                     /locus_tag="PLT31_00975"
     CDS             <1..1220
                     /locus_tag="PLT31_00975"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="spermine synthase"
                     /protein_id="HPW93736.1"
                     /translation="LSDPLLKKLDCVEIEEEMMNLAKGFMPYNERGYNDPRINIFIDD
                     ARTFFHTHKQKYDVIISVPSNPWVSGVSSLFSHEFYTQMKRYLKPGAQWVQWIQTYEF
                     NDMLFLNILKALDQSFKYVSLYQTPEEPDVIIIASDSPVQQQYIKRFESDSILLKEFE
                     RIKRPWYFFGEQNFLFTTKMISPLLKDIAPNSEFIPIVDNKAEEARFTHSEAKITKAF
                     DSCEVCWPDVLDSNDFAARRAFKNFTISKEPKNNYLKTSLMAHLNDTAFVEHAKKEPY
                     NKEWLTFYDEYNEYIFSIPLEERDSCELYQKVRDLIYTDSMPEAQTLQFKMMESLRLK
                     KYTEAAENLNAFVEKYEIKDMNEFFLRNSALISILAKEYKLLDYIYKEAIRHHDNFSV
                     PEKVLIELKIKQK"
     gene            complement(1403..1858)
                     /locus_tag="PLT31_00980"
     CDS             complement(1403..1858)
                     /locus_tag="PLT31_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93737.1"
                     /translation="MKRIEKIRHLFIDVSPIERLLEFSTPYYPVVSGVLDIVYEKKIK
                     MIVSPLTLQRVSYRAFLTQNDVLARQYREFFTRSDLLYLREVDSEIALKAAEFQAKYS
                     LNMDESIQLATAFWSGADGILTENQEWKNYLDISVITLQDLLSSEQFNI"
     gene            complement(1861..2868)
                     /locus_tag="PLT31_00985"
     CDS             complement(1861..2868)
                     /locus_tag="PLT31_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93738.1"
                     /translation="MIFKKILLFVCFLTALSFSQSLTIYRKATDESSPLGTLSYSDWV
                     KELPIKPKKVKKTYYVKNKKGKKVKKVKYETVTPKEPPRFIPVKCKFGSGYANRAELA
                     RFFERSKDISGTYSSKTGTAVLQKSPNNPGKFSLVILNGTETERAEFEVGNLELKEAN
                     GHSRFRFQEPDCRLDIDYFNRKITILQNGCDAYSTPNFKLGGEYDNYTEIIRRAETFH
                     FPETKYTFKKFLWCPEGPDFCEKTKDENGKVEIVWSVNGEGLIERRSGDQIHTYRPYE
                     RMIPNRSDFYDGEKPIALKTKRTDMSSEWMLWLYYPKAERFKMVRIGTRNDAAYTEIY
                     E"
     gene            2970..4523
                     /locus_tag="PLT31_00990"
     CDS             2970..4523
                     /locus_tag="PLT31_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93739.1"
                     /translation="MKKTIFISLLLATFALAAEPATAVKTTTPTNAATVKNEPVKAAP
                     ATDSKAQAVTAPAKENVVADTSAKAEATPAKALPAADSNAQAATAPAKENVVADTSAK
                     AEATPAKALPAADSNAQAATAPAKENVVADTSAKAEVTPAKAEPAVSATEKTAATPTK
                     NKKKVKKAKKVKAKKAPLITAGAFKFDMNSDFEIQAGKVLWASEDDKNRYNMEEWFGR
                     ANLAFFTQSENFDGKISMSVYPGDLIDKKTVVANSDTTPKETEMREVIELNEAWAWQR
                     TKYLNFKLGRWENTDKNGDYFGGYVDGYLNGFKSTFDPENMIQFGFTPTENLSVDLAL
                     ISTGPYLNTGDLRIVAHFSQLSSIEALNIDLALRTNIFDKVYDHESDVRTNISVKVNI
                     PLLEDKLFLFGEVAMLGLGGDSTELYIDNNGIVKERDVVVDWKMPITGGLMLKNTVVD
                     RIILEAEYIADRSESEYHSNSKHIKDVLGAFYIEKDLTSRFTLSAGVHNHGSSKDWVI
                     NGNLIGRIN"
     gene            4604..6655
                     /gene="metG"
                     /locus_tag="PLT31_00995"
     CDS             4604..6655
                     /gene="metG"
                     /locus_tag="PLT31_00995"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HPW93740.1"
                     /translation="MKNFYITTPIYYVNDAPHIGHSYTTVLADILTRFHKLIGYQTFF
                     LTGTDEHGQKVQQAAAKRQVDPQIHVDEYNQRFKKLWEKMNIEYNYFIRTTDADHKEF
                     VAECLQKLWDKGEIYSKEYEGWYSVGEERFFSEEELVDGKDPISGRPVEWLKEKNYFF
                     KMGMYQQKLITHLEENPDWILPDYRRNEILGFLKQPLNDLCISRPKSRLSWGIPLPFD
                     DEYVTYVWFDALINYVSGVRNRAYENGEPIWPASYHLIGKDILTTHCVYWPTMLMALE
                     IPLPKHVLAHGWWLTGGSKMSKSSGTGVNPMDYMEQFGVDAFRYFLAREMVVGQDCTF
                     SDDAFKRRINSDLANDLGNVLNRVHRLVLTNFEGKLPKATQIDAPAQELITLANQVRE
                     QVFSGITQVKLSQVIEDIMSLVRGINRYLEIKAPWKLAKDPSAKDELASVLFTAAEAV
                     RLSLCFLWPVMPTKTMEGLAMLGTTCEGEKDLHWGKFQGGESFGEGAPLFPRIEVEKA
                     PPPPAKTKEQNKPVLATDVPSLLDLRCAKIISVEDHPDAESLYVLKVDAGESEVRTIC
                     SGLKKSYTPEELKDRLILLFANLKPAPLRGIMSMGMLLAGDGEEGKAVLVDPPANTPI
                     GTRALFKGIIPSESRELKIKDFDKISLYVKDKTIFCNDNRLEFNGTPVSCDVKDEASV
                     H"
     gene            6879..7064
                     /locus_tag="PLT31_01000"
     CDS             6879..7064
                     /locus_tag="PLT31_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93741.1"
                     /translation="MKTIICNIKTNVFNYIRTLNWKMLLILGVFCIVLAVLNNIFVDE
                     SKSVEWIGSQPVLEVPE"
     gene            7067..7726
                     /locus_tag="PLT31_01005"
     CDS             7067..7726
                     /locus_tag="PLT31_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014544958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HPW93742.1"
                     /translation="MIIAIISLSIATLLTAFGVAEISFPETFLTITDKDWLLEIFPTA
                     WKYSVHIGVFSIVIAALLIIPAWKSDRAFSEKALETLLRLGLGGMFIAASIFKIQDPL
                     AFATLVAQYQFLPEFITNFFALAYPQFELWFGLALIFTPYTRESALVIFLMFISFIIA
                     LSWALFHDLGITCGCFQIEGAQDKSETWTSLIRDLVLIGPNFWLMLRKNKSLIRLWRE
                     K"
     gene            complement(7809..8276)
                     /locus_tag="PLT31_01010"
     CDS             complement(7809..8276)
                     /locus_tag="PLT31_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93743.1"
                     /translation="MTKPIDNDMDDFDEPLLIDEEEDIEEVPAVDQTTAVRDYSDRRV
                     LVWEEDDERCGITVEVLSDLLVGAYVKGVKTEIEALALLDSEEWDTFVVDFYTEGVSA
                     SEFIKRVNNYPDAILVAIAMTPLRLLEEREPAKLESLRRLFDYEKNSNAQIRG"
     gene            complement(8298..8708)
                     /gene="ruvX"
                     /locus_tag="PLT31_01015"
     CDS             complement(8298..8708)
                     /gene="ruvX"
                     /locus_tag="PLT31_01015"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HPW93744.1"
                     /translation="MKYLCIDFGERRVGLAVGDSELRFAFPRETIDRQIQDLWSRLAE
                     IVTNEKIDSFVVGMPFHPDGRKDGKNIVVEAFIEELKEHFPEKSIFTQDESYSSVQAL
                     QQTSYMSRKKKKDKGIVDRAAAAIILQNWLDGNI"
     gene            8800..10632
                     /gene="typA"
                     /locus_tag="PLT31_01020"
     CDS             8800..10632
                     /gene="typA"
                     /locus_tag="PLT31_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HPW93745.1"
                     /translation="MDTSKIRNIAIIAHVDHGKTTLVDQLLKQCGSFHENEEVEERVM
                     DSDNLERERGITILSKNAHVNYKGYSVNIVDTPGHADFGGQVERVLGTVDGVLLVVDA
                     FEGPMAQTRFVTQKALELGLIPIIVVNKIDRDGCNPHSALNKVFDLFCELDANEEQLD
                     FAHVFGSGRKGICRRELSDPDGDFRILMDLILERIPGPKGDPNAAPLLQIASLEYSGF
                     LGRLAVGRVKEGTFKPNLTVSQSLEDGTFRNIRIQKILRYEGVTPKPIEEAGPGDIIL
                     IAGLDKFDIGDTLSSVANPVHLPRIHIDPPTISMLFTVNTSPLVGKYGGKFLTGNHLL
                     ERLERAHMSDPALLVEKVDGASTFKVSGRGILHLTILVENMRREGYEFTIGSPQVIFQ
                     KDSTGKLLEPVESFKVELPSEYSGAVIEELGKRKGEMQNMLQDENNRVFLEYTIPSRG
                     LIGLRPILLSLSKGYAVSQSIFLEYQSFKGDIPARTNGVMIAKDAGDAASYALSKLED
                     RGYMIIPPGAEVYPGMIVGEHNRNVDIIVNVTKGKHLTNMRASGSDDNIQLTPYRRLT
                     LEECVTFINDDECIEVTPEILRIRKATLDPHKRKQESKVQTEEE"
     gene            complement(10635..11387)
                     /locus_tag="PLT31_01025"
     CDS             complement(10635..11387)
                     /locus_tag="PLT31_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HPW93746.1"
                     /translation="MDINNKMPFALTVAGFDGSGGAGILADIKAFESFGVYGQAVCTA
                     NTIQNESEFMDSGFLPWDTIVAQLDALYRRRFFDFVKIGLIPSAEILKNIVEYIRKHS
                     PNAFIIWDPILSATAGYHFFSAQEVESFLPWLNQINLITPNQKEFTYLGLGLAASRNE
                     VKIGSDFAVLLKGGHQKGDFSTDTLWYEDQQIKFSSKRLSGKGKHGTGCTLSAAILAN
                     LALGYSLVESCQIAKNYMDLFLRGGEGLLGRV"
     gene            complement(11426..12034)
                     /locus_tag="PLT31_01030"
     CDS             complement(11426..12034)
                     /locus_tag="PLT31_01030"
                     /inference="COORDINATES: protein motif:HMM:NF014622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HPW93747.1"
                     /translation="MAFKNNLVLITSPEDFADEHILIERMFEQGLSRLHVRKPGRSKR
                     DLDYWLLGLGYDYRKHCVLHSDLGTALDLEVGGVHTKTLLPSDSILLQKPESFSYSCS
                     CHSLKELNELPSYVDYAFLSPIYDSISKVGYKSAFSIDELKSFFSGVVEKKSIPVYAL
                     GGVDKDNLEELEQIGFAGAALLGGIWHYADPLQAWAEIIRSK"
     gene            complement(12034..13497)
                     /gene="guaB"
                     /locus_tag="PLT31_01035"
     CDS             complement(12034..13497)
                     /gene="guaB"
                     /locus_tag="PLT31_01035"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPW93748.1"
                     /translation="MRIINEEALTFDDVLLVPGDSNVLPAKTDVSTSLSPNLKLNIPI
                     VSAAMDTVTTAPLAISLALLGGIGVIHKNMSIEAQAEEVRAVKRWQSGIVFNPVTLDA
                     DQPVSDAFEMRAKLKVSGFPILSKGKLVGMLTSRDLRTITNTNMKISEVMTKDLITAS
                     PKVKLSKAKEILNSNRIEKLPLVDQAGNLKGLVTMTDILKRESNPAASLDKNEQLLVA
                     AAVSTSADSLDRAKALVEAGVDMLVVDTAHGHHIGVRNMVKTLRKKYPKLTICGGNVA
                     TPEAVTELAKCGADIVKVGIGPGSICTTRVVAGVGVPQFTAIVQCAQAARKAGISIIA
                     DGGIKYSGDIAKALAAGANAVMMGSLFAGTEESPGEIILADGRSFKSYRGMGSIGAMK
                     DGSKDRYFQSDIKEEKKFVPEGIEGRVAYKGPLRDTIYQLVGGIHQAMGYVGAANLHE
                     MFKKATFVRITNAGLRESHPHDVTITKEAPNYRTSES"
     gene            complement(13542..14102)
                     /gene="pth"
                     /locus_tag="PLT31_01040"
     CDS             complement(13542..14102)
                     /gene="pth"
                     /locus_tag="PLT31_01040"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPW93749.1"
                     /translation="MYLIVGLGNPSSQYENTRHNAGFLAVEKLADPSANWKSEHHALT
                     QKIKIGSEEVLLAKPQTFMNLSGDAVLPLLSWYKIPLTQLIVFVDDVQLDCGRIRVRA
                     KGSHGGQNGLRHISEKVGDSFSRVRFGVGKCPPQWNLADWVLSKFTKEDAEIFNSTLK
                     HVSPLVSLWLSKGIGACMDRYNGPLD"
     gene            complement(14174..14752)
                     /locus_tag="PLT31_01045"
     CDS             complement(14174..14752)
                     /locus_tag="PLT31_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HPW93750.1"
                     /translation="MEFTSLKATSRVLGAGRETKRLRKTGQIPAVYYGKGQEPVHLSV
                     SAIDVRKVLAPGKRYTLLDLEIDGKSGNPALIYVYQKDSISQEITHIDFLRIDENTSV
                     LVRIPVRLSGIPVGVKAEGGMLSQENYYIKLLSPVTKIPPFIEIDISDVHAGSTYYAE
                     NLTLESGVSLVSKKRTVIFAISKARAKEAASN"
     gene            complement(14779..14850)
                     /locus_tag="PLT31_01050"
     tRNA            complement(14779..14850)
                     /locus_tag="PLT31_01050"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14816..14818),aa:Gln,seq:ttg)
     gene            complement(14901..15749)
                     /gene="ispE"
                     /locus_tag="PLT31_01055"
     CDS             complement(14901..15749)
                     /gene="ispE"
                     /locus_tag="PLT31_01055"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HPW93751.1"
                     /translation="MIEKEYAPAKINLFLDILRKRPDGYHDLGTAFQTVDAGDTLEAS
                     LRKDSEITLSYNAKQDYPIESDLVYKAAHLLQSTYKVSMGVDLYLSKIMPIGAGLGGG
                     SADAAAALRLLNRLWGLSLSSKELELLAVRLGADVPFLIDGGLSFAEGIGEKLTPMPP
                     LLLPESMALLIATPQCAVPTKAAYAGVKPSGDDRWDEWKSKYDSSVMPELFNKFEESV
                     LPQFPLIAEMKKDFLSYGAIASLMSGSGASVFGFFENRREADLALSKLGNRARWATVT
                     TFLKKM"
     gene            complement(15746..18358)
                     /locus_tag="PLT31_01060"
     CDS             complement(15746..18358)
                     /locus_tag="PLT31_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HPW93752.1"
                     /translation="MAVKTKKRKATKKKDGEQGLWSIITGWSLFAFGFLLLLSAMTVR
                     YSGGAENWLGPYLGLLFPESMTFIFGRVAVLIFAIAAIVWGLWFILNMKHFRLFRLAG
                     GILALSLTTSFLLSLRVYEVLHPSKTILFDNGGLVGSFLAQDIARPVFGNSPLAPALI
                     GFFMLLVIAVFAFGLRLHHFSFLKVFFDKMLASWKTFQEKRRAKQESKEALEAEAEKE
                     KRALLLDDFTIVKTGKAKPFKSTDSWCDSFVKSANTETLVDTHTDATATPVEDQEDEI
                     AEKERFLLENEDRLGVREKRMLRDEIAELRRIQEQNIWEDKRRERPAIEGFVRREMDF
                     QAEEKTIPMQVNEEETIVPTAVESEIELEVINEEEPSSPAAEEPTVYDVYKLPIVSDI
                     LPPSPDQTADYSEEELQQTAEMLEFQLENFKVKGKVVGISTGPLVTRFEVEPGPGVKV
                     SRFSALQEDLALALKAMSIRILAPIPGKSVVGIEIPNRKMHTVYSRDILESPLFAPKP
                     DKILIVLGKDIMGNPFTMDLARAPHILIAGQTGSGKSVCINVLMASILFSKTPDELRL
                     ILVDPKVVELKLYEKIPHLLHPVITQPEVAVQALKWACVEMDRRYEVLALARVRNIAG
                     FNAKVEANELDEDFPEEERKRMPFLVIVIDELADLMMVAGKEVETSIARIAQKARAVG
                     IHLVLATQRPSVNVITGLIKANLPTRISFKVASQIDARTVMDKAGAEKLLGRGDMLFR
                     ATEDPEPHRVHGAFLSDSEAEKLADVCSNQNVFYPKVESFDLESEEVSDDPQGNVKLD
                     SRIGEVIGWCINVKGVSVSAVQRSFSVGFSRAGKIVDQLEYLGVCGKSKGNSKPRDIL
                     MTEEELLNLMRTLP"
     gene            18609..>19652
                     /locus_tag="PLT31_01065"
     CDS             18609..>19652
                     /locus_tag="PLT31_01065"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HPW93753.1"
                     /translation="MSWKQTKSLKKVIVAISLTSLIYFGISWVGSSAEYHTNRLNSWI
                     QTLENIFYDSFFKWKTNEPETDETTENVTHTENYDPHIFIIDIDEPSLSKLGNYNEWQ
                     RKIHADVIENLSKGGASAIGFDILFKNADFGNKKAEQTISVLKEAFPEENWDHQFPFI
                     KNKYNDDSILIERIERNKNVILCASFDEKEAYKHESQWRPLSDKQRQLSLGTYSTFNL
                     SQVDHPENIEPKDLLDNLFPELAQATPHMGVINAYPDPDGVVRHVSTMYRFPNPELYP
                     NEKNLIHYTMSISTLLHLFHQDPKNVKVKMGEYIRTEPTLIEPLPFLYCMLYYRDINS
                     PNATGIVIKDIINA"
BASE COUNT         6358 a         3910 c         3430 g         5954 t
ORIGIN      
        1 tgttatcaga ccctttgcta aaaaaactcg attgcgttga aattgaagaa gaaatgatga
       61 acctcgccaa aggctttatg ccctataacg aaaggggtta taatgaccct cgaatcaata
      121 ttttcattga tgatgcaaga actttcttcc atacccataa gcaaaaatat gatgtaatca
      181 tcagtgtgcc ttctaaccct tgggtcagtg gcgtctctag tttatttagc catgaatttt
      241 atactcagat gaagcgctac ttaaaacctg gcgctcaatg ggtgcaatgg attcaaactt
      301 atgagtttaa tgatatgctc tttttgaata tcttaaaagc gttagaccaa tcatttaaat
      361 acgttagttt gtatcaaacc ccagaagaac ctgatgttat tattattgct agcgactccc
      421 ctgtacagca acaatacatt aagcgttttg aatctgactc tattttgtta aaagaatttg
      481 agcgaatcaa gcgcccatgg tatttctttg gagaacaaaa cttcttattt accacaaaga
      541 tgatttcccc tttattaaaa gacattgctc ctaatagtga gtttattcct attgtggata
      601 ataaagcgga agaagctcgc tttacccatt cagaagcaaa aatcactaaa gcctttgact
      661 cctgtgaagt atgctggcca gatgttttgg attcaaatga cttcgctgca cgtagagcat
      721 ttaagaactt tactatatcc aaagagccta agaataatta tttgaaaaca agtcttatgg
      781 ctcacttaaa tgacaccgct tttgtggaac acgccaaaaa agaaccttat aataaagaat
      841 ggttaacatt ctatgatgag tacaacgaat acattttttc aattcctcta gaagaacgag
      901 actcatgtga attatatcag aaagtaagag atttgattta caccgattct atgccagagg
      961 cacaaaccct tcaatttaag atgatggaaa gtttaaggct caagaaatat acggaagctg
     1021 cagagaactt aaacgctttt gttgaaaaat atgaaatcaa agatatgaat gaattcttct
     1081 tacgaaatag tgccctaatt agtattctcg ccaaagaata caaactcctt gattatatct
     1141 ataaagaagc tattcgccat cacgataatt tttcagtccc agaaaaagta ttaatcgaac
     1201 taaaaatcaa acagaaataa atttggagtt taggccacct gtgtagcata ttctttgccg
     1261 aattgatcaa acgagtgcga agaattgtta ccagattttt aaaaatgaaa taacgtgcta
     1321 aatgagtatc ataaaaacat ttgcattttc atgaccgaat aacaccatca aaagtatgtg
     1381 taaaaattta tagagaagaa ctttaaatat taaattgctc tgaagagagt aaatcttgga
     1441 gggtaatcac tgaaatatct aaataatttt tccattcttg attttctgtt aaaatgccat
     1501 ctgcaccact ccaaaaagca gtcgctaact ggatcgattc atccatattc aaagaatatt
     1561 ttgcttgaaa ttcagcggcc ttcaaagcga tttctgaatc tacttctctt agataaagta
     1621 aatcggatcg agtaaaaaac tcgcggtatt gacgagctaa aacatcgttt tgagttaaga
     1681 acgctctata ggaaactcgt tgtaatgtta aaggtgagac tatcatcttg attttttttt
     1741 cataaacaat gtctagtaca cctgagacca ctggataata gggagtacta aattccaata
     1801 gcctctcaat cggagataca tctataaaca aatgacgtat tttttctatt cgcttcattt
     1861 ttactcgtat atttcagtgt atgccgcatc attccttgtc ccaatgcgca ccatcttaaa
     1921 acgttctgct tttggataat atagccaaag catccattca cttgacatat cggtacgctt
     1981 cgtttttaaa gcaattggtt tttcaccatc ataaaaatca gatcgattag gaatcattcg
     2041 ttcatacgga cgatacgtat gaatttgatc tccactccga cgttcgatca agccttcccc
     2101 atttacgctc cacacaattt ccacttttcc attttcatcc ttagttttct cacaaaaatc
     2161 aggaccttca gggcaccata aaaatttctt gaaagtatac ttcgtttctg ggaaatggaa
     2221 tgtttctgca cgacgaatta tttctgtata attatcgtat tctcccccga gcttaaagtt
     2281 cggtgtagaa tatgcatcac agccgttttg cagaatcgtt attttcctat tgaaataatc
     2341 gatgtctaaa cgacaatctg gttcttgaaa acggaatcga ctatgaccat tggcttcttt
     2401 aagctctaaa ttaccgacct caaattcagc acgttctgtt tccgttccat ttaaaatcac
     2461 taaagaaaat ttaccagggt tatttggaga cttttgtaaa acagcggttc ctgttttaga
     2521 agaataagta ccactaatat ccttagaacg ttcaaaaaag cgagcaagtt ctgcacgatt
     2581 agcgtatccc gaaccaaact tgcattttac aggaataaat cgcggtggtt ctttaggagt
     2641 aacggtctca tattttactt tttttacttt cttccccttt ttatttttca cataatatgt
     2701 tttctttact tttttgggtt taataggtag ttcttttacc cagtcagaat aactcagagt
     2761 acccaaggga gaagattcgt ctgtagcctt acgataaatg gtcaagctct gcgaaaaaga
     2821 taaagctgtt aaaaagcaaa caaataagag tattttttta aaaatcatga gaagaacata
     2881 atattttaga acctttttgt tttttttatg aaaaaaaaaa ctgttttttt actaaaattt
     2941 acattacgct ttattctagg agaccgaata tgaaaaagac tatttttatt tctcttttat
     3001 tagccacgtt tgctcttgct gcagaaccag caactgcggt taaaacaaca acccctacaa
     3061 acgctgcaac tgtaaaaaat gaacctgtaa aagcagctcc tgctacagac tctaaagcac
     3121 aagccgttac agctcctgct aaagaaaatg tagtagctga tacttctgct aaagctgaag
     3181 ctacccctgc taaagcttta cctgctgctg attctaatgc acaagccgca acagctcctg
     3241 ctaaagaaaa tgtagtagct gatacttctg ctaaagctga agctacccct gctaaagctt
     3301 tacctgctgc tgattctaat gcacaagccg caacagctcc tgctaaagaa aatgtagtag
     3361 ctgatacttc tgctaaagct gaagttactc ctgctaaagc tgaaccggct gtatctgcta
     3421 cagaaaagac tgcagctact cctactaaga ataagaaaaa agtaaaaaaa gctaaaaaag
     3481 taaaagctaa aaaagctccg cttattaccg ctggtgcttt caagtttgat atgaattcag
     3541 atttcgaaat tcaagctggt aaagttctat gggcttccga agacgataaa aataggtata
     3601 acatggaaga atggtttggt cgtgctaatc tagcgttctt tacacaatca gaaaactttg
     3661 atggaaaaat ttctatgtct gtatatccag gcgatcttat cgacaaaaaa accgtcgttg
     3721 ctaattcaga cacgactcct aaagaaacag aaatgcgcga ggtcatagag ctcaatgaag
     3781 catgggcatg gcaacgcact aaatacctta attttaaatt aggtcgttgg gaaaatacag
     3841 ataaaaatgg tgattatttc ggtggatacg ttgatggcta tttaaatgga ttcaaatcta
     3901 cttttgaccc agaaaacatg attcaatttg gttttacacc aacagaaaat ctctctgtag
     3961 atttggcatt aattagcact ggtccttatt tgaatacagg tgatcttcgt attgtagcac
     4021 atttcagcca attgtcttct attgaagcac tcaatattga tcttgcttta cgtactaata
     4081 ttttcgataa agtatacgat catgaaagtg atgttcgtac caatatttcg gtaaaagtaa
     4141 atattcctct cctcgaagat aagttattct tatttggtga agtggcaatg cttggcttag
     4201 gtggagattc cactgaactt tacattgata acaatggtat tgttaaagaa agagatgttg
     4261 ttgtagattg gaaaatgcct ataactggtg gtcttatgct taaaaacact gttgttgaca
     4321 gaatcattct tgaagcagaa tatattgccg atcgttctga atcagaatat catagcaact
     4381 caaagcatat taaagatgtt cttggcgcat tttatattga aaaagatctt accagtcgat
     4441 tcactttatc tgcaggcgtt cataaccacg gcagttcaaa agactgggta atcaatggta
     4501 atttgattgg tcgtattaac taaataacct aataagactt tttatataaa ccattttaaa
     4561 gcttccttct acaggaagct ttttcttttt ctatatttga gtcatgaaaa atttttatat
     4621 cactacccca atttattacg ttaatgacgc ccctcatatt gggcactctt atactactgt
     4681 tttagccgat atcttaactc gttttcataa gctaattggt tatcaaacct tttttctaac
     4741 tggtacagat gaacatggtc aaaaagtaca acaagctgcc gcaaaaagac aagtagatcc
     4801 tcaaattcat gttgatgagt ataaccaacg ttttaaaaaa ctttgggaga aaatgaatat
     4861 tgagtataat tattttatcc gtaccacaga tgctgatcat aaagaatttg tcgctgaatg
     4921 cttacaaaag ctatgggata aaggggaaat atactccaaa gaatacgaag gttggtattc
     4981 tgtcggtgaa gaacgctttt ttagtgaaga agaactagtc gatggcaaag accccatcag
     5041 tggacgacct gtagaatggt taaaagaaaa aaattatttc ttcaaaatgg gaatgtacca
     5101 acaaaaattg attactcatc ttgaagaaaa tcctgattgg attcttcctg attatcgtag
     5161 aaacgaaatt ttagggttct taaaacaacc tcttaatgat ctttgcatta gccgccctaa
     5221 atctcgttta agctggggaa ttccccttcc atttgatgac gaatacgtca cctatgtttg
     5281 gtttgatgct ctaatcaact atgttagtgg cgttagaaat agagcttatg agaatggaga
     5341 gcctatttgg ccagcttctt atcacttaat aggtaaagat attctcacaa cacattgcgt
     5401 ctactggcca accatgctta tggctcttga aatcccttta ccaaagcatg ttttagcaca
     5461 tggatggtgg ttgactggtg gctcaaaaat gagtaaatcc agcggcacag gtgtaaatcc
     5521 aatggattac atggaacaat ttggagtcga tgcatttcgc tattttcttg caagagaaat
     5581 ggttgtaggc caagattgta cttttagtga tgatgctttt aagcgtcgaa ttaacagtga
     5641 tttagcaaac gatttaggca atgtcttaaa ccgagtccat cgtttagttc taacaaactt
     5701 tgaaggcaag cttccaaagg caactcaaat agatgcacct gctcaagaat taatcactct
     5761 tgcgaatcaa gttagagaac aagttttttc agggattact caagtcaagt tgtctcaagt
     5821 gattgaagat attatgagtc ttgttcgagg aatcaatcgc tatctggaaa ttaaagctcc
     5881 atggaaacta gcaaaagacc catctgcaaa agacgaactc gcctctgttc tatttactgc
     5941 agccgaagcc gttcgtttat ctttatgctt tttatggcct gttatgccta ctaaaactat
     6001 ggaaggctta gccatgctcg ggactacttg tgaaggtgaa aaagatcttc attggggtaa
     6061 attccagggt ggagaatcct ttggagaagg agctccatta tttccaagaa ttgaagtgga
     6121 aaaagcacct cctcccccag caaaaacaaa agaacaaaat aaaccagtac ttgcaacaga
     6181 tgtgccttcc ttattagatc ttagatgcgc caaaatcatt tctgtagaag atcatcccga
     6241 tgcagaaagt ctttacgtct tgaaagtcga tgcaggcgaa agtgaagttc ggacgatttg
     6301 ttctgggctt aaaaagagtt atactccaga agagctcaaa gaccgtctta ttttactttt
     6361 tgccaattta aagccagctc ctcttcgcgg gattatgagc atgggaatgc ttcttgctgg
     6421 agatggtgaa gaagggaaag ccgttctagt ggatccacca gcaaatactc caattggcac
     6481 tagagcttta tttaagggaa ttattccttc tgaaagcaga gaattaaaaa taaaagactt
     6541 cgacaaaata tctctttacg ttaaagacaa gactattttc tgtaacgata atcgtcttga
     6601 atttaatggg acacccgttt cttgcgatgt caaagacgaa gcctcagttc actagaatct
     6661 gaattcaata tcattatttt cgagcaaaaa aattattttt tttgctcttt ttttttgaat
     6721 ctgtatttga aagcagaaaa aaaacgaagc tagttctctt aatccattct attttgtctt
     6781 tcgccaacca atttttagaa aaactctatt gattttttca ttgggttatt ttttttcgaa
     6841 atatggtttt cataaaatca atttgttaat ttgtgcttat gaaaacaatt atatgtaata
     6901 ttaaaacaaa cgtatttaat tacattagaa ccttaaattg gaaaatgctt ttaattttag
     6961 gcgttttttg tattgtttta gcggttctaa acaatatttt cgtagatgaa tctaaatctg
     7021 tagaatggat aggttctcaa cctgttttag aagtaccgga gtaactatga ttatagccat
     7081 tatttcttta tctatagcaa cgctactcac tgcctttggt gtcgccgaaa tttcttttcc
     7141 agaaactttc ttaaccatta cagacaaaga ctggttatta gaaatattcc ctactgcgtg
     7201 gaaatatagt gttcacatag gcgtattttc catagtcatt gcagcacttt tgatcattcc
     7261 tgcttggaaa tcagatcgag cgttttctga aaaagctctt gaaaccttat taagactagg
     7321 tttaggtggt atgtttatcg cagcaagtat ctttaaaatt caagatccac ttgcatttgc
     7381 tacgttagtc gctcaatacc aatttttacc agaattcatc actaactttt ttgcactagc
     7441 ttatccacaa tttgagcttt ggtttgggct tgcgctaata tttacgcctt atacaagaga
     7501 atctgcttta gtcattttct taatgttcat ttcttttatt attgctcttt cttgggcatt
     7561 atttcacgac cttggaatta cgtgcggttg cttccaaatc gaaggggctc aagataaaag
     7621 cgaaacttgg acttctttaa ttcgagattt agttttaatc ggccccaatt tctggttaat
     7681 gctcagaaaa aacaagagct taattcgtct ttggcgcgaa aagtaagatt catttatact
     7741 actttcaaaa aaaacagacc ttttaaaagg tctgtttttt taatgaatca aaattaatat
     7801 taaataaatt agccacgaat ttgcgcgtta gaatttttct cgtaatcaaa taaacgacgt
     7861 agagattcta acttagcagg ttcacgctct tctagaagcc ttaaaggcgt catggcaatg
     7921 gccactaaaa tagcatctgg gtaattatta actcgtttga taaactcact agcagaaaca
     7981 ccttctgtgt aaaaatcaac gacaaaagta tcccattcct cggagtctaa taaagcaaga
     8041 gcctctattt cggttttaac acctttcaca taagcaccca ctaataaatc agaaagcacc
     8101 tcaacagtga taccacaacg ttcatcgtct tcttcccaaa ctaaaacacg gcgatcgcta
     8161 taatcacgaa ccgcagttgt ttgatccaca gcaggaactt cttcaatgtc ttcttcttca
     8221 tctataagaa gaggttcatc aaaatcatcc atatcattat ctataggttt tgtcataatg
     8281 ctctcaaata taacaactta tatatttccg tctaaccagt tttgcaaaat aatagccgct
     8341 gcagctctat ctacaattcc tttatctttt tttttctttc gactcatata agaagtttgc
     8401 tgtaaagctt gtacgctcga atacgactca tcttgagtaa aaatggactt ttcagggaaa
     8461 tgttccttta attcttctat aaaagcctct actacaatat tcttaccatc ttttcgacca
     8521 tctggatgaa aaggcattcc tacaacaaaa ctgtcaattt tttcatttgt tacaatttca
     8581 gcaagtctag accaaagatc ctgtatttgc ctatcaattg tttctcttgg aaaggcaaaa
     8641 cgtagttctg agtcgccaac agcgagtcct acacgccttt caccgaaatc aatacataaa
     8701 tatttcatag aaacaaatat aaaattgcaa aatagttttg ttatttctaa atttcgcgcg
     8761 gctatagatt ttacttagct cttttatcaa aagcgaatta tggatacatc aaaaatcaga
     8821 aatatagcga tcatcgctca cgtagaccac ggtaaaacta ctttggttga ccaactccta
     8881 aaacaatgtg gctcttttca tgaaaatgaa gaagtcgaag aacgcgttat ggactccgac
     8941 aatctggaaa gagaacgcgg tattaccatc ttatcaaaaa acgcacacgt caattacaaa
     9001 ggttatagcg ttaatattgt ggatacacca gggcatgctg attttggtgg tcaagtagaa
     9061 cgggtgttag gtactgtaga cggagtgctt cttgttgtcg atgcctttga agggccaatg
     9121 gcacaaactc gtttcgttac acagaaagca ttagaattag gtttgattcc tattattgtt
     9181 gttaataaaa ttgaccgtga tggatgtaat cctcattctg ctttaaataa agtttttgac
     9241 ctcttctgcg aattagatgc aaacgaggaa cagcttgatt ttgcacatgt cttcggcagt
     9301 ggacgtaaag gcatttgtcg tcgtgaactt agtgatccag atggcgactt tagaattttg
     9361 atggatctca ttttagagcg cattcctggg cctaaagggg atcctaatgc agctccccta
     9421 ttacaaattg cctctttaga atactctggc tttttaggtc gcttagctgt tggtcgcgta
     9481 aaagaaggta ctttcaaacc taatttaaca gtatcacagt ctttagaaga tggtactttc
     9541 cgaaatattc gaattcaaaa aatccttcgg tatgaaggag ttaccccaaa accaattgaa
     9601 gaagctggcc caggagatat catcctcatt gcggggttag ataaatttga tattggagat
     9661 accctttctt cagtcgcaaa tccagtacat ttaccacgta ttcatataga tcctccaacg
     9721 atttctatgc ttttcaccgt gaatacctct cctctagtag gaaaatacgg tggtaagttt
     9781 ttaacaggaa atcacttgtt ggaacgctta gaaagagctc atatgtcaga cccagcgctt
     9841 ttagttgaaa aagtagatgg tgctagcaca tttaaggttt ctggtcgtgg cattcttcac
     9901 ttaacgattc ttgttgaaaa tatgcgtcgt gaaggatatg aatttaccat tggtagtcca
     9961 caggttattt tccaaaaaga ttccacagga aaattacttg aaccagtaga atcttttaag
    10021 gtagaattac catctgaata tagtggtgct gttattgaag aattaggcaa acgcaaaggc
    10081 gaaatgcaaa acatgcttca agatgagaat aatcgtgttt tcttagaata tactattcca
    10141 agccgtggtc ttattggtct tcgccctatt cttctatctc tatctaaagg ttatgcggtt
    10201 tctcagtcta tcttcttaga atatcaatct tttaaaggtg acattcccgc tcgaaccaat
    10261 ggcgtcatga tagctaaaga tgctggagac gcagcaagct atgcattatc taagctagaa
    10321 gaccgtggat acatgattat tcctccagga gcagaagtgt atccaggtat gattgttggc
    10381 gaacataacc gcaatgtgga tattattgtt aacgtcacta aaggaaagca tctcaccaac
    10441 atgcgtgctt ctggctctga tgataatatt caattgactc cttatcgccg actcactcta
    10501 gaagaatgcg ttacttttat caatgatgat gaatgtattg aagtgacacc tgaaatactt
    10561 cgtattcgta aagccacttt agatccacat aaacgtaaac aagaatctaa agtacaaaca
    10621 gaagaagaat aagattagac tcttcctaaa aggccttctc cgcctcttag gaagagatcc
    10681 atgtaattct tggctatttg gcaagattcc actaaagaat aacctaaggc taaattggct
    10741 aaaatcgctg ctgataaagt acagcctgta ccatgtttac ctttgccaga taatcgtttt
    10801 gaagaaaatt taatttgttg atcttcatac cataatgtat ctgtggaaaa atctcccttt
    10861 tgatgccctc cttttaatag aacggcaaaa tcagatccaa ttttcacttc atttcgagag
    10921 gcggcaagac caagcccaag atacgtaaac tctttttgat tgggcgtaat cagattaatt
    10981 tgatttaacc atggtaaaaa agattcgact tcttgagctg aaaaaaaatg atacccagcg
    11041 gtagcagaaa gaattggatc ccaaataata aatgcgtttg gagaatgttt tcgaatgtac
    11101 tccactatat ttttcaaaat ttcggcagaa ggaataaggc ctattttgac aaaatcaaaa
    11161 aaacgcctgc gatataatgc atctaactga gctacaatgg tatcccatgg caaaaaaccg
    11221 gaatccataa attcagattc attttgaatt gtatttgcag tacagaccgc ttgcccataa
    11281 acgccaaaag attcaaaggc tttaatatcg gctaaaatgc ccgcaccacc agagccatca
    11341 aaccccgcga cggttaaagc aaaaggcatt ttattattga tatccacaaa gaagaaaata
    11401 gtattatttt ttaaaataaa tacttttatt ttgatctaat aatttcagcc caagcttgaa
    11461 gtggatccgc ataatgccaa atgccaccaa gcaaagccgc ccctgcaaaa cctatttgtt
    11521 ctaattcttc gagattatct ttatccactc cacctaacgc ataaacagga atagattttt
    11581 tttctacgac gccggaaaaa aatgatttta attcatcaat agaaaaagcg gatttataac
    11641 caactttaga aatagaatca taaataggac ttaaaaaagc ataatcaaca tacgaaggca
    11701 attcatttaa ttcttttaat gaatggcaag aacagctata agaaaatgat tctggcttct
    11761 gaagtaaaat ggaatcagaa ggtaatagag ttttggtgtg aactccccct acttcaagat
    11821 ctagagcagt acccaaatca ctatgcaaaa cacaatgttt gcgataatca taccccaaac
    11881 ctaataacca ataatctaaa tctcttttag aacgacctgg ttttctaaca tgaagtctag
    11941 ataaaccttg ctcaaacatc ctttcaataa gaatatgttc gtcagcaaag tcttctggac
    12001 ttgtgattaa aaccagatta ttcttaaaag ccattaagat tcagaagtcc tgtagttagg
    12061 agcttcttta gtaatggtta catcatgtgg atgagattca cgaaggccag catttgtaat
    12121 tcgtacaaaa gtggcttttt taaacatttc gtgtaaatta gccgcaccaa cataacccat
    12181 cgcttgatga ataccaccaa ccaactggta aatagtgtcg cgaagaggac ctttataggc
    12241 cacgcgacct tcgattcctt cgggaacaaa cttcttttct tctttaatat cggactggaa
    12301 gtaacggtct ttagaaccgt ctttcatggc accaatggaa cccatgccac gatagctttt
    12361 gaagcttctg ccgtcagcaa ggataatttc accaggactt tcttcggttc cagcaaagag
    12421 gctacccatc atcaccgcat ttgctcctgc ggcaagtgct ttggctatat cgccagaata
    12481 tttaatgccc ccatcagcaa taatagagat gcccgcttta cgagctgctt gtgcacactg
    12541 cacaatagct gtaaactgag gaacacctac accagcaact acacgcgtgg tgcaaataga
    12601 accaggacca ataccgactt taacaatatc agcgccacat ttggcaagct cggtgaccgc
    12661 ttctggagta gcaacattac cgccacaaat cgtgagcttt gggtattttt tacgaagagt
    12721 tttaaccatg ttgcgaacac caatatgatg cccatgagcc gtatcgacca caagcatatc
    12781 gacaccagct tccacaagtg cttttgctct atctaaagaa tcagcagatg tacttactgc
    12841 agcagcaact aaaagttgct cattcttatc aagacttgca gcagggttac tttctctctt
    12901 taagatatcg gtcatagtca caagaccttt tagattacca gcttgatcta cgagaggaag
    12961 tttttctatg cgattggagt ttaagatttc tttagctttg gataatttaa cttttggcga
    13021 agcggtgatc aaatctttcg tcatcacttc agaaattttc atattcgtat tagtaattgt
    13081 tcttaaatca cggcttgtaa gcatacctac taactttcct tttgaaagaa tagggaaacc
    13141 gcttactttt aatttagcgc gcatttcaaa agcatcactc acaggttgat cagcatctaa
    13201 agtcactgga ttaaaaacaa tgccagactg ccaacgcttg acagcgcgaa cttcttcggc
    13261 ttgtgcttca atagacatgt ttttatgtat aaccccaatt ccacctaata aagcaagtga
    13321 aatagcaaga ggagctgttg tcactgtatc cattgccgca ctgacaattg gaatattcag
    13381 ctttaaattg ggcgataaag aagtactgac atcagtcttt gctggtaaaa cattagaatc
    13441 accaggaaca agcaatacat cgtcaaaagt taaagcttct tcattgatta tacgcataca
    13501 tacctctcct ttctggattc tatcccagaa aataaaaaaa attaatctaa aggaccgtta
    13561 taacgatcca tacatgctcc aattccctta gaaagccata aagaaactaa aggagaaaca
    13621 tgtttaaggg tggaattaaa aatttcagca tcttcttttg tgaatttaga taaaacccaa
    13681 tcagctaaat tccattgagg aggacacttc cccaccccga aacgcactct tgaaaaagaa
    13741 tcgcccactt tttcactaat atgacgtaat ccattttgcc cgccatgact tccttttgcc
    13801 cgaacacgaa ttcgaccgca atcgagttga acatcatcta caaaaacgat caattgagtt
    13861 aatgggattt tataccaaga taataaaggt aaaacagcat cgccactaag attcataaat
    13921 gtttgtggtt ttgctaaaag gacctcttct gaaccaattt ttattttttg agtaagagca
    13981 tgatgttctg atttccagtt ggcagatgga tcggcaagct tttccacagc aagaaaaccc
    14041 gcgttatgac gagtattctc atattgagaa gaaggattgc ctaaaccaac gatcaaatac
    14101 atcaaaaacc tctctataca agagaaggct caccgcaagg cgagccttca taaaaaacct
    14161 tcaaaaccct tgcttagttt gaagcggctt ctttcgcgcg tgcttttgaa atagcaaaaa
    14221 tcacggtgcg ttttttagaa actaaagaga caccactttc taatgttaag ttttctgcat
    14281 aataagtaga accagcatga acatcagaaa tatcaatttc gataaatgga ggaatcttcg
    14341 ttacaggaga taataactta atatagtagt tttcctgaga aagcatacca ccttctgcct
    14401 taacaccaac aggaatacct gataagcgaa caggaatacg tacaagaact gatgtatttt
    14461 catcaattct taaaaaatca atatgtgtaa tttcttgaga aatggaatct ttttggtaga
    14521 cataaataag agcgggattt ccggacttac cgtcaatttc aagatccaaa agcgtataac
    14581 gcttgccagg ggctaggacc ttgcgtacat caatagcgct aacgctaaga tgaactggct
    14641 cctgaccctt accataatag acagccggaa tctgaccggt cttacgcaag cgttttgttt
    14701 cgcggcctgc gcctaatact ctcgaggttg ctttgagcga agtgaattcc atgataactc
    14761 cagtaaaaaa aaagcctatt ggggtagctg gattcgaacc aacgaataac ggaatcaaaa
    14821 tccgttgtct taccacttga cgatacccca tagggagtcc caaagatagt agaattcaaa
    14881 ataaatttaa aggcttattt tcacattttt tttaaaaaag tggtcactgt tgcccaacga
    14941 gcccgatttc ccaatttcga gagagctaaa tcggcctctc gacggttttc gaaaaagcca
    15001 aaaacagatg ctcctgaacc agacatcaaa gaagcgatag ccccatagga taaaaagtcc
    15061 tttttcatct cggcaatcaa tggaaattga ggtaaaacag attcttcaaa cttattgaat
    15121 aattctggca ttacagatga atcatatttg cttttccatt cgtcccaacg atcgtctcca
    15181 gaaggcttta cacctgcgta agctgctttt gtaggaacag cgcactgagg cgttgcaatc
    15241 aatagagcca ttgattctgg aagtaaaaga ggaggcatcg gtgttaattt ttctccaatg
    15301 ccttctgcaa aggaaagccc accatcaatt aagaaaggaa catctgcacc aagacgaaca
    15361 gctaaaagtt ctagctcttt tgatgataaa gaaagacccc ataaacgatt gagaagacga
    15421 agagctgcag cggcatctgc actaccacca ccaagaccag ccccaatagg catgatttta
    15481 gaaaggtaaa ggtccacacc catcgacact ttgtatgtac tttgcaggag atgagctgcc
    15541 ttatatacta aatctgattc tataggataa tcttgctttg cattatagga aagcgtaatc
    15601 tctgaatcct ttcgaagaga ggcttctaaa gtatctcctg catccacagt ttgaaaagct
    15661 gttcctaaat catgatagcc atcggggcgt ttacgaagaa tatctaaaaa taaattgatc
    15721 ttcgctggag cgtattcttt ttctatcatg gtaaagttct catcaaatta agaagttctt
    15781 cttccgtcat taaaatatct ctaggtttgg aattaccttt actctttcca caaacaccaa
    15841 gatattcaag ttgatcaaca atttttcctg ctcggctaaa gcctacacta aaagagcgct
    15901 ggactgccga aacagaaaca cctttcacgt ttatacacca tccaatgact tctccgatcc
    15961 tactatccaa tttcacgttt ccttgaggat catcagaaac ttcttcggat tctaaatcaa
    16021 acgattctac tttaggataa aaaacatttt gattagaaca tacatcagct aatttttcag
    16081 cctccgaatc agagaggaaa gccccatgca cacgatgtgg ttcagggtct tctgtcgcac
    16141 ggaaaagcat gtctccccga ccaagaagtt tttctgcacc cgctttatcc atcacagtac
    16201 gagcatcaat ctgagaagca accttaaaac taattcgagt cggtaaattg gctttaatca
    16261 aacccgtaat cacattgact gaaggccttt gagttgctaa aaccaagtga attccaacag
    16321 cacgtgcttt ttgagcaata cgagcaatag atgtttccac ttcttttcct gcaaccatca
    16381 tcaagtcagc aagttcatcg atcacaatca caaggaatgg catgcgttta cgttcttctt
    16441 caggaaaatc ttcatctagt tcatttgctt ccactttcgc attgaatcca gcaatgttac
    16501 gcactcgagc tagagccaat acttcataac gtcgatccat ttctacacac gcccatttta
    16561 aagcttgaac agctacttct ggttgtgtaa tcacaggatg aagcagatga gggatctttt
    16621 catataattt taattcaacg actttagggt ctactaatat caaacgaagt tcatcgggtg
    16681 ttttactgaa tagtatacta gccatcaaaa cgttgataca aactgatttt ccagaacccg
    16741 tttgccctgc aatcaaaata tgaggggccc ttgctaaatc catggtaaaa gggtttccca
    16801 taatatcttt gccaagcaca attaaaattt tgtctggctt tggagcaaaa agagggcttt
    16861 ctaaaatatc tcgagagtaa accgtatgca ttttgcgatt tggaatttca attcccacca
    16921 cagattttcc aggaatagga gctaaaattc gaatcgacat ggccttaagt gcaagagcta
    16981 aatcttcttg taatgcagag aaacgagaaa cttttacacc tggaccaggt tccacttcaa
    17041 aacgagtgac taaaggcccc gtagaaatgc caacgacctt tcctttgacc ttaaaatttt
    17101 ccaactgaaa ttcaagcatc tcagccgttt gctgtagttc ttcttcggaa taatccgctg
    17161 tttgatctgg tgagggcgga agaatatctg aaacgatagg aagcttatag acatcataaa
    17221 ccgtgggctc ttccgcagct ggagaagaag gttcctcttc attaattact tctaattcaa
    17281 tctcgctttc tacagcagtc gggacaatcg tttcttcttc gtttacttgc ataggaatcg
    17341 ttttttcttc tgcttgaaaa tccatttcac gacggacaaa gccctcgata gcaggccttt
    17401 ctctgcgttt atcttcccat atattttgtt cttgaatacg acgtaactca gcaatttcat
    17461 cgcgaagcat acgcttttca cgaaccccta aacgatcttc attttctaat aaaaaacgtt
    17521 ccttctctgc aatttcatct tcttggtctt ctactggagt tgcagtggca tccgtatgag
    17581 tatcaaccaa tgtttctgta tttgctgatt ttacaaatga atcacaccaa gaatcggtgg
    17641 atttgaaagg ttttgctttt cctgttttaa caattgtaaa atcatctaac aagagagccc
    17701 ttttttcttt ttcagcttct gcttctaagg cttcctttga ttcttgttta gctcttcttt
    17761 tttcttgaaa cgttttccaa gaggcgagca ttttatcaaa aaagactttc aaaaaagaga
    17821 aatgatgtag acgtaaacca aaagcaaaaa cagctatcac taaaagcatg aaaaagccaa
    17881 tcaatgcagg agcaagcggc gaatttccaa agacagggcg tgcgatatct tgagctagaa
    17941 agctccccac taacccccca ttatcaaata aaatcgtttt tgaaggatga agcacttcat
    18001 aaacacgtag agaaagtaaa aatgacgttg ttaaagaaag ggctaaaata ccaccagcga
    18061 gccgaaacaa acggaaatgc ttcatattta gaataaacca caagccccaa acgatagccg
    18121 ctatcgcaaa aataagaaca gccacacgtc caaaaataaa cgtcatagat tctgggaata
    18181 aaagcccaag ataaggccca agccagtttt cagcaccacc agaatatcgg acagtcatcg
    18241 ccgaaagcaa gagaagaaaa ccaaaagcaa aaagagacca accagtaata atactccaaa
    18301 ggccttgttc gccatctttt ttcttagtcg ccttgcgctt ttttgtttta acagccaatt
    18361 aaaaactcca tcaagtaaat aaaaatctaa tcattcaaaa atgactattt agagaaatcc
    18421 ttaatcgaat ttacaaaaaa aatagcctaa ggtcaccacc taaaactctt tttctatgaa
    18481 aacgcaacgg aacaccgtgt gtgtttgcat tttagtattc ctgtttattt cacaaacaaa
    18541 ataaggctaa tgctattttt tgtaaaaagg tctttttagt aaaacttttc ttttactatg
    18601 ttttcaatat gtcgtggaag caaactaaaa gtctaaaaaa agtaatcgta gctatttctc
    18661 tcacctcttt aatctatttt gggatttcat gggttggctc gtctgcagag tatcatacca
    18721 ataggctaaa ttcttggatt caaactcttg aaaacatctt ttacgattcc ttttttaaat
    18781 ggaaaacaaa tgaacccgaa actgatgaaa caacagaaaa cgtcacccat acagaaaact
    18841 atgaccctca tattttcatt atagatattg atgaaccttc tttaagtaag ctcggaaact
    18901 ataatgaatg gcaaaggaaa atccacgctg atgtgattga gaacctttct aagggaggag
    18961 cttcggcgat tgggtttgat attttattta aaaatgctga ttttggaaat aaaaaagcag
    19021 aacaaactat aagtgttctt aaagaagctt tcccagaaga aaattgggat catcaatttc
    19081 cgtttataaa aaataaatat aacgatgact ccatccttat tgaacgaatt gaaagaaata
    19141 aaaacgtgat tctctgtgct tcttttgatg aaaaagaagc ttataaacac gaatctcaat
    19201 ggaggccttt aagtgataag caaagacaac tttctttagg cacttattct acgtttaatt
    19261 tatcacaagt agaccatcct gaaaatatag aacctaaaga cttattagat aatttatttc
    19321 ctgaattagc acaagcaact cctcacatgg gtgtaattaa cgcttaccca gaccccgatg
    19381 gcgttgttcg acatgtttct acgatgtatc gatttccaaa tcctgaactc taccctaacg
    19441 aaaaaaattt aatccattat accatgtcca ttagcaccct tttacacttg ttccatcaag
    19501 accccaaaaa tgtaaaagtt aagatgggtg aatacataag aacagagccg acactaatcg
    19561 agcctttgcc gttcctttac tgtatgcttt attatagaga tataaattcg ccgaatgcta
    19621 caggtatcgt tataaaagat attattaacg ca
//
LOCUS       DAOOBB010000010        19642 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_3679_length_19642_cov_4.398683, whole genome shotgun sequence.
ACCESSION   DAOOBB010000010 DAOOBB010000000
VERSION     DAOOBB010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 19642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19642
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3679_length_19642_cov_4.398683"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..162)
                     /locus_tag="PLT31_01070"
     tRNA            complement(90..162)
                     /locus_tag="PLT31_01070"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(126..128),aa:Glu,seq:ctc)
     gene            complement(188..2161)
                     /locus_tag="PLT31_01075"
     CDS             complement(188..2161)
                     /locus_tag="PLT31_01075"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPW93754.1"
                     /translation="MLTWINEKAKWIIVVFAVGIVLGLLAMDRLPDQAIQYPIGKVDG
                     EKISYENFDSRLKMIVANRYEGVHLEEEQYSKLRQDLFQSFVRQHLLTQVMEDAELEA
                     SVVEMKAEIQRNPDLIRGMVGQEAQQRIYAIQSSATSAEDANQRMQAYIATLPKFLLD
                     STFDKASFDQWLNTPEAYEWQAMMNYEKDLKSSSIPLKQMQIFVTANLHPTSLESRYN
                     VERRLTDYDLEVAVVNGSDFVAPESSVDSVVISAYFNAFDSFYVEKDLMKLKYAVLPI
                     EPTATDEAKIREYAMTLYFQLTDSTSTTNFEELARITSEDPASAEQGGALGDYTARGA
                     WVKEFEDVAFSLDSGAISEPVRTKYGFHIIQSHGKKTDSTGVELVKASHILLTVTASS
                     ETIDSLENIMEDVRTAFQSSENFDAAAKTKNLQVLSSDWLSKGDQISSLGYVSGLTAY
                     AFANKERPETSSGPISSVLKNKNFVVIASKTDSLVAGSRSLNAFYNDIKSKLLQKKSA
                     ENAAAYLNSVAAKIKEQAVAKVDSTAQDSLAQDSASATMPVIEKVKFEKTVASVDGFV
                     PGFGFSNPMLSSVLKNQKVGEWGPVITTDRGAVMLKINSKKQPSEDAVEAAIKEELGN
                     VFRFDASVAFNSYVTSLEAGAKVEDNLDLYYKD"
     gene            2296..3876
                     /gene="nadB"
                     /locus_tag="PLT31_01080"
     CDS             2296..3876
                     /gene="nadB"
                     /locus_tag="PLT31_01080"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014544880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HPW93755.1"
                     /translation="MYDILVIGAGVSGLSAALHAADKGLSVILLTKGAKPDGSSNYAQ
                     GGIATVTAKTDSFQLHINDTLEAGAGLCKKEVVRILTQNGPSTIRQLVKWGVKFTPAE
                     KNEDNIPFDLHLEGGHSHKRILHAADLTGKEMMRALLFELHSRPNIHFIENSVAYDLI
                     CDGEGENKRCIGAKIIHQKIGKMQAIFAKATIIATGGAGRLWLHTTNPADSSGEGMAI
                     ASRAGAALQDIEFMQFHPTSLYAPKLKKTFLISEAVRGFGGILKNKEGEEFMNSVHPL
                     RSLAPRDIVARAIHREMAKTGEPNMFLDVTARSPKDIKTQFPNIYAKCLEVGIDMTKD
                     WIPVVPAAHYMCGGILVDSWSRTEIRGLYACGEVAATGVHGANRLASNSLLESVIFAL
                     RAIENILKSNLISSDFTPPKIRLEKIVLTDGNIWKQRRLILQKTMWNYCGIVRTVEGL
                     NQGLNVIYEFEKEINKTLVKKGPKNIDFLAFRNAVQNSKLILKSALCRKESRGLHYIL
                     DYPDLDPKTKHHRIYLDD"
     gene            3905..4933
                     /locus_tag="PLT31_01085"
     CDS             3905..4933
                     /locus_tag="PLT31_01085"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPW93756.1"
                     /translation="MATQSSSGKSRKKSKPEKIGGYSPTQKIGQGAMGDIWLCHDPSL
                     DRMVIVKQMIPNLTAYEELNLRFQREASLLAHLKHPNIVHAYSLWQERTGRLSLAMEF
                     IHGKTLREILDVSPTPPLFATLYFLHEILQVLAYAHQRKIIHRDLKPSNMMVDKIGRI
                     KLLDFGIARNAGATQDMTLPGSVLGTAAYMSPEQITGKKVTLHSDLFSLGIIAFEMLT
                     GKHPFRGETMELTTQYILNVKIHKNLFPKQVPHALRRFVCKILEKKPSKRFQSAQEAA
                     DELSKIMEGFPRQLDVYMGQWLDSLSHPESKFEPPPQKKHKSYLYLAVGFITGIIVAA
                     STSLFILN"
     gene            4940..5521
                     /locus_tag="PLT31_01090"
     CDS             4940..5521
                     /locus_tag="PLT31_01090"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HPW93757.1"
                     /translation="MNWIDITCLIIVTLFTFLGIWAGLFKSIFKLVAWIIAAIGAYYG
                     YDIAGPFISSNFDLTQTTIRMICVIGGFLIPLFSIYTFIFILDRWIKKTPLNGLNRLG
                     GALFGLAKSLIICFVLLTIIHYLPFSGSLHQTRNSSISYELYKELAAKDISTQISMIQ
                     TKAAEEVKKLQEQNPEKKESSSSKEDSSKSQRK"
     gene            complement(5578..6825)
                     /locus_tag="PLT31_01095"
     CDS             complement(5578..6825)
                     /locus_tag="PLT31_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPW93758.1"
                     /translation="MPPLLIKSVWKDHDLVDIFINEHYFQKIAKSISLDEVPKNTRVI
                     DGAEKAIIPPFYNAHTHAAMTLLRGYADDMSLFKWLSEYIWPLEAKLTREDVYWGSRL
                     AILEMIKSGTVFFSDMYWNREETIRAVIEMGVRATIGVTFAENLMSPKSIEDNFDFLE
                     KNAACHERIQLAVMPHAVYTVGDVLWRRCAETAQRLGLVLHTHLAETTEEVENCIQKT
                     GKTPVQYLDSLGVLNQNVVLAHAIHLTPEDIDILQKRDVTLVHNPSSNMKLSSGKMNI
                     SALMKAGIPIALGTDGASSNNNLDMREEMKFAALLAKVDGGPELLSADEVFHMATVQG
                     SRAYQVNAGLIEEGKLADAILLDLNNERLIPNHHLVSNWVYAADARCIDKVICHGQIL
                     MENKSVENEIEILERVKKFAAKN"
     gene            6951..7946
                     /locus_tag="PLT31_01100"
     CDS             6951..7946
                     /locus_tag="PLT31_01100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPW93759.1"
                     /translation="MFEENISKFPISKQEFSRLKVFHNVCYESVIGYLLHSSIDEVEQ
                     GFVLIDPSKTDKKLVVLLEGKLEVHIDSIDGVLADYIEEGGCAGEMSIFDNCNPSAWV
                     ISSTKCKVLIISAPTTLAMLNASHDLCLNFLHLLSQRVRYSNKVVCEDQYHIRCIEQY
                     SQIDTLTSLHNRRWLEEMYTRELKRSHAGDFRLCAFMIDIDFFKRINDTYGHLAGDVV
                     LVEIARTLNESLRPSDMPVRFGGEEFSVFLPGTTSENAKVIAERIRANIEKKEIKLPS
                     GAIVHVTVSIGFAEREKSDSVESLIGKADKALYQAKQHNRNRICMYVSKDETFLF"
     gene            complement(7938..9362)
                     /locus_tag="PLT31_01105"
     CDS             complement(7938..9362)
                     /locus_tag="PLT31_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HPW93760.1"
                     /translation="MSSIPLSESYFFIGLAGSGMSAIAQYLAGKGHSIGGSDRQFSST
                     TPPLVQKQLQEMGIQCFLQDGSGVSATYSAVVVSSAIEDTNPDIVQAKALGLKILHRS
                     EILALISKSVKTIAVSGTSGKSTVTAMIFHVLDYAGLSPSMITGAGLVSLEKKGKIGN
                     AVAGFGEWLVVEADESDGTLIRYEPEIGLILNLDKDHKELTELNEIFTTFSHHVVDRG
                     KTLIVNDAHPRAKEFSAGREFDFGSENHVGVQGTDFSAIGTGIRFRVRYQNELVRFDL
                     PFPGRHNMENALAATAVALRVGVSLKTISEALSSFSGIYRRHQILGTFSGITLVDDFA
                     HNPAKIAASLRSVEAFTEGRVIAWFQPHGYGPTRFLRKDLVSEIKGALRVSNTDSQND
                     CIFFSDIYYAGGTVVKDISSADLVDDLLEEGVEAHYFADRKECAKEMVRLAEPSDCIL
                     LMGARDPSLGEFALYVKELLENLK"
     gene            complement(9365..10630)
                     /locus_tag="PLT31_01110"
     CDS             complement(9365..10630)
                     /locus_tag="PLT31_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPW93761.1"
                     /translation="MICFKVGESQAEERIDRFLQAQLKDFSRTDIQKLIAAGSVLVNG
                     STISKNFRLEPEMEIQVLALPKKEESNLEPEDIPLNIIYEDDDIVVINKPRQLVVHPG
                     SGVRTGTLAAGLLYHFKENLSQVNGPLRPGIVHRLDKDTPGLMVVAKNDKAHLSLASQ
                     LENRTLHRTYKALVWGQPRDLEGTIDAPIDRDPKNRLKMGIVWGGKEARTHYKILEFF
                     TFASLLELELETGRTHQIRVHNRYMGNPVFGDPLYDGRDNCLMRVPPLVRDVASEALE
                     KSPAQLLQAVKLSLIHPSTGKMVTFEAPLEKPFEDVLKYLRKEAPCDNPSFNQTFFKD
                     FESDRRYIDVEEVVEEDPYLYEEEPVKERMTRAERLLKKKERIARRKEFEIEKRRKKE
                     EREAIRRGEIPSEYIVPGYEPTIDPNLLG"
     gene            complement(10627..11208)
                     /gene="lspA"
                     /locus_tag="PLT31_01115"
     CDS             complement(10627..11208)
                     /gene="lspA"
                     /locus_tag="PLT31_01115"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HPW93762.1"
                     /translation="MNNNWINKIKTLKFQIGLGIFGLIADQITKIWAVLRFTNEDGTL
                     TREMIPIITIANEPLLRFQLVYNEGAAFSSRPQDIMPFLPPVVFFLLMSIIALSVLIW
                     FFRTIDYRDWVSRLGVIMIISGALGNFIDRMRLQKVVDFIDCDFPDFIMARFPTFNVA
                     DSFVTVGVILVFLSPVLLKKIHQQIKLEKEIKK"
     gene            complement(11205..12251)
                     /locus_tag="PLT31_01120"
     CDS             complement(11205..12251)
                     /locus_tag="PLT31_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HPW93763.1"
                     /translation="MIKKNSPDFCFAKNILLQVSRTFALNINVLSGLLHQSVLLAYLF
                     LRIADTIEDDPDLAATEKEKLLKYFASIFETPEVDPKRVDLFLSMLPSQWIQSKDPNH
                     LLTCRTPVVIGLWSELPLGNRKIIQKVIIEMCQGMAKFALKQEQQLAQGWFTIKKTSE
                     LDQYCYYVAGIVGKMLSQLFFIDSRFIDVETFDDLKKWDVSFGLALQVTNIIKDVRED
                     ASRKVCFIPEEICWRHGLKNSYDIFEPDVSPDVRSAIMKELVDKAWSHLDDSIRYVLK
                     IPRVDRRIRLFCLWPLFMAAENLSLIGDGKIIFDSEEKAKISRSTVKKIIKDTSLHFY
                     SNRWIEKEYSRLRP"
     gene            complement(12244..12819)
                     /locus_tag="PLT31_01125"
     CDS             complement(12244..12819)
                     /locus_tag="PLT31_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93764.1"
                     /translation="MISWIKIFGQYFWLGLLFWAGIFLWFRYRAYPLYLKKMLRTGKN
                     WVYIPLWWKNNQKKHMQIKFVGVLLLLLTIFFSASSLYWLIPLSSWVFFVGLAFFTIL
                     AVFFARWSIRYVGNLEQECYFFEYRKQVFKDQKEGKKIQDTDIRNRTTWSFQSHLKRA
                     DEHGRFLQYIVSMSKSQKKPKDLYAEAMFHD"
     gene            12913..14229
                     /gene="dnaA"
                     /locus_tag="PLT31_01130"
     CDS             12913..14229
                     /gene="dnaA"
                     /locus_tag="PLT31_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPW93765.1"
                     /translation="MQTQWEKSLNVLRNKISSSIFITLFSKLKIASLNNNHATIIAPS
                     DLDLKEIAPYKLLTEISWEEANGKHVDLDFRHASVLVEPSSYSNSSIKEFTPSVPLNS
                     IYRFENFIPGDKAQLAFNAAFAVAENPDGNKYNPLFIYGASGLGKTHLLQAIGNYVLE
                     SDNPKRVRYLTAYDFQQQYIKSVRDQRITEMSAFYRNEVDVLLIDDIQNWSGKDETQN
                     EFFHIFNALHQAGKQIVLTSDAPAVEVKSLSDRLVSRFSWGLTVDIQPPNLETREAIL
                     RKKAEEKHIDIDDEVILYLAENIESNVRFLESAVIKLTVQASIMHHDIDIHLARKVVA
                     DIVPTIRRRVSMDSIVNAVSRHFEVPEEKLLAPGRGTKEIAHARQVAMFLMKELTTLS
                     LQSIGTRFGGKDHSTVVHAIKTIRKEMEATPAFERTIENLKAEIHD"
     gene            complement(14260..14829)
                     /gene="lptE"
                     /locus_tag="PLT31_01135"
     CDS             complement(14260..14829)
                     /gene="lptE"
                     /locus_tag="PLT31_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HPW93766.1"
                     /translation="MTNRYQVLFRFLSFVGLSPNTCRRVGFIGSSIIIIGFLFILSGC
                     YSFTASTLPSHIKTVHIFEVDDKTLDPVLANNLRTGILELFRKNAGSVRIVNDEASAD
                     FKISLLSYSNKPDNYNSSDQVETYRVTIRVSVLFYDNVKDRVIYEEKNLSADGIYDVQ
                     ANETEDRHGQTRAIEKLQDLIITNALAKW"
     gene            14957..16231
                     /gene="serS"
                     /locus_tag="PLT31_01140"
     CDS             14957..16231
                     /gene="serS"
                     /locus_tag="PLT31_01140"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPW93767.1"
                     /translation="MLDIRKIRENPEFYHIETQKKGSSIDLMSVLAIDEKRRTLLTEV
                     EKLKSERNAESKRIGEAKKRGENADEAVQAMRVLGDKIAALDNEMKELDNEQTEKLMH
                     IPNVPHSSTPEGKDSSDNVVQSSWGTPKEHSFNAVDHKTLGEKLGLFDFERGAKISGS
                     GFPVYRGLGARLERALIQWFLDSHREAGFEEIIPPYLVTRNTMRGTGQLPKFEEDMYR
                     CDKDDDLFLIPTAEVPVTNLYADEVIPEAELPKRLCAYSACFRREAGSYGKDTRGLLR
                     LHQFNKVEMVYFAHPEKSYEEHENLTRFGEILLEKLGLPYRRLLLCKGDLGFGAAKCY
                     DLEVYAPVEKKWLEVSSCSNFEDFQARRANIRTKINGKNTFLHTLNGSGLATPRIMVG
                     ICDNYQQPDGSLVIPEVLRPYMGGLEKIEPSL"
     gene            16253..17137
                     /locus_tag="PLT31_01145"
     CDS             16253..17137
                     /locus_tag="PLT31_01145"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPW93768.1"
                     /translation="MTKKFDTPITEVPLFHQGKVRDMYDLGDSFLMVASDRLSAFDVV
                     LPTPIPGKGKILNQLSLFWFKLLDMPNHLITANVDEYPEVLKKHADYLRGRSMIVKKA
                     HRHSVECIVRGYIVGSGWKDYQKTGKICGHQLPPNLQLCQKLEQALYTPSTKPDVGHD
                     ENISYEETFDIVGEKVAKTLKDLSLDIYTKARDYAASKGIILADTKFEFGEIDGKTIL
                     IDEVLTPDSSRYWPADKYEIGKNQESFDKQYIRDWLETLDWDKTDPGPEIPPEIVKKT
                     LEKYIEIFVRLTGKDPVL"
     gene            17411..19297
                     /locus_tag="PLT31_01150"
     CDS             17411..19297
                     /locus_tag="PLT31_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPW93769.1"
                     /translation="MNKIKLGILGTGYIATKMAEAVKYLENHSMGIEAYAVASRNKSK
                     ADQFVQEHRFQKAYGSYEDLVNDPIVHLIYIATPHSEHFENAKLCIEKGKAVLVEKAF
                     TSNAKQGSILISLAEEKGVLLVEAMWTRFLPAIKIIKDWITAGRIGEVKLIEADFSQP
                     ISHIERLQNPSLAGGALLDLGIYCLTFADLFLEGNIISTQSHCIPFKTGVDASDWITL
                     TYSSGQKAFLKTSMVTPPKNEGILYGSKGYIRVENLNNMVRLELLDHTSQLLETIVPP
                     MLMNGYEYQLLACKKALEAKPRIEGNRKIWECEEMPHSKTLSMMEQLDSLRDSFGVTY
                     PFEIPNSKIWDRSKNKSILQIYNIETKECIRLKEFDYVIEAPNWSTNGDFLTYNSNGK
                     IFKYDITTATITEVPSYYIDQCNNDHVLAPDGSGLYVSHHTKEDGLSRIYRIFFDGRK
                     PILITPLGPSYLHGVSPDGKTLAYCAARNGEYDIYTIPSEGGDETQLTSTKGLNDGPE
                     YDSTGEYIWFNSVRTGRMQAYRMKANGEEQTQITEDLHWNTWFPHISPDKKHAVMIAY
                     HENDVKPGEHVPNKNVEIRLIKVEGNQWSSPETILKLFGGQGTINVNSWAPDSKRFAF
                     VSYL"
BASE COUNT         6475 a         3877 c         3400 g         5890 t
ORIGIN      
        1 gaaggcttat tttggaccga gagcctagct ctcggttttg aagaaataca aaaaacctcg
       61 ctactttttc gtagcgaggt taataaaaat agcgtgtatg ggactcgaac ccatgttacc
      121 ggcgtgagag gccagcgtcc tagaccacta gacgaacacg ccaaatggtg cgacaataat
      181 ataaaaatta gtctttatag taaaggtcta aattatcttc tacttttgca ccagcttcca
      241 aagaagttac ataactatta aacgcgacgc ttgcatcaaa gcggaacaca tttcctaatt
      301 cttctttaat tgcagcttca actgcgtctt ctgatggttg ctttttagaa ttaatcttta
      361 gcatgacagc gccacgatct gttgtgatta ctggtcccca ttcccctacc ttttgatttt
      421 tcaaaacaga ggaaagcatt ggattactaa atccaaatcc tggaacaaag ccatcaacag
      481 aagcaacagt cttttcaaat ttgacttttt caataacagg cattgtagca cttgctgaat
      541 cttgagctaa cgaatcttga gcagtgctat cgaccttagc cacagcttgt tctttaattt
      601 ttgcagcgac agaatttaag taagcagcag cattttcagc tgacttcttc tgaagaagct
      661 ttgatttaat atcgttatag aacgcattta agctacgaga gccagccact aaactatctg
      721 ttttagaagc aattactaca aaatttttgt tctttaatac acttgaaata ggaccagatg
      781 aagtttctgg acgttcctta tttgcaaaag cataagcagt aagaccagaa acataaccta
      841 agctagagat ctgatcacct ttgctcaacc agtctgagct aagaacctga agattctttg
      901 tttttgcagc agcatcaaaa ttttctgagc tctggaaggc tgttctaacg tcttccatga
      961 tgttttcaag actatcaatt gtttcggaag aagctgttac tgttaaaaga atatgagaag
     1021 ctttaactaa ctcaacacct gtagagtctg tttttttacc atgagattga atgatatgga
     1081 aaccatactt tgtacgaaca ggttcagaaa tagctcctga atctaaagag aaagcaacat
     1141 cttcaaattc tttgacccat gcaccacgtg cagtatagtc tcctaaagca ccaccttgtt
     1201 ctgcactagc tggatcttcc gaagtaattc gagcaagttc ttcaaagttt gtagtacttg
     1261 tagaatcagt caattggaaa tacaaggtca ttgcatattc tctgattttt gcttcatctg
     1321 tagctgtagg ttcaataggt aaaacggcat actttaattt cattaaatct ttttcaacat
     1381 agaagctatc aaaagcgttg aaataagcag aaataacaac actatccaca gaagactctg
     1441 gagcgacaaa atcagagcca tttacaacag caacttcaag atcataatca gttaaacgac
     1501 gttctacatt atagcgagat tcaagtgatg ttggatgcaa attagcagta acaaaaattt
     1561 gcatttgttt tagaggaata gaggacgatt taagatcctt ctcataattc atcatggctt
     1621 gccattcata agcttcagga gtatttaacc actgatcaaa tgaagcttta tcgaaagtag
     1681 agtctaaaag gaattttggc aaagtggcaa tataggcttg catgcgttgg tttgcgtctt
     1741 ccgcactagt tgcagaagat tgaatagcat aaatgcgttg ttgagcttct tgcccaacca
     1801 taccacggat aagatctggg ttacgttgga tttcagcttt catttcaaca acagaagctt
     1861 ctagttcagc atcttccatg acttgagtaa gtaaatgttg acgaacgaag gattgaaata
     1921 aatcttgcct taacttagaa tactgttctt cttctaaatg aactccctca taacgatttg
     1981 caacaatcat ttttaagcga gagtcaaaat tttcgtatga tattttttca ccatccactt
     2041 ttcctatcgg gtactggata gcctggtctg gaagacgatc catcgctaaa agacctaaaa
     2101 caatgcctac ggcaaagaca acgataatcc attttgcttt ttcattaatc cacgttaaca
     2161 tagagaaaac tccataaaaa ttgacgaggg tcaatgtagc aataaattat cctttctgta
     2221 aaataaatct agttttatac cccctttttt tctatttatt cccctgcatt tcactcttag
     2281 cctcagaggc ctaaattgta tgatatttta gtgattggcg caggtgtttc tggtttaagt
     2341 gcagcccttc acgctgcaga taaagggctt tcagtcattc tcttgaccaa gggagctaaa
     2401 ccagacggtt cttcaaacta tgcgcaaggt ggcattgcta ctgttaccgc taaaacagac
     2461 tcttttcaac tacatatcaa cgatacacta gaagctggtg ctgggctatg taaaaaagaa
     2521 gtagtccgta ttttaaccca aaatggtccc tcaactattc gtcaacttgt aaaatgggga
     2581 gtaaaattta ctccagcaga aaaaaatgaa gataatatcc ctttcgactt acatttagaa
     2641 ggtgggcata gccataaaag aattcttcat gcagcagatc tcactggaaa agaaatgatg
     2701 agagccttgc tatttgaact tcattcaagg cctaatattc attttattga aaactctgtg
     2761 gcttatgatt taatctgtga cggagagggt gaaaacaaaa ggtgtatcgg ggcaaaaatc
     2821 attcatcaaa aaataggaaa gatgcaagcg atttttgcta aagcgacaat catagcaact
     2881 ggaggagctg gacgcctctg gctccatacc actaatccag ccgatagttc tggagagggc
     2941 atggccattg catcgcgcgc gggggccgct cttcaagata ttgaattcat gcagtttcac
     3001 cccacctctc tttatgcacc aaaattaaaa aagacttttc tcatttccga agccgttcgt
     3061 ggttttggcg gaattctaaa aaataaagag ggtgaagaat ttatgaattc cgttcaccca
     3121 cttcgctctc ttgcacctag agatattgtg gcaagagcca ttcatcgtga aatggcaaaa
     3181 acaggtgagc caaacatgtt tttagatgtg accgcaagat ctcctaaaga cattaagacg
     3241 caattcccaa atatttatgc taaatgttta gaagtcggaa ttgatatgac gaaagactgg
     3301 attcctgttg ttcctgcggc tcactatatg tgtggcggca ttcttgtaga ctcttggtct
     3361 cgcacggaaa taagaggttt atatgcctgt ggtgaagtcg ctgcaacagg agttcatggg
     3421 gcaaacagac ttgcttccaa ctctcttcta gaaagcgtta ttttcgctct tcgcgctata
     3481 gaaaacattc ttaaaagtaa tttgatcagc agtgatttta cacctccaaa aattcgttta
     3541 gaaaaaattg ttctcactga cggaaatata tggaaacaac gacgtttgat ccttcaaaaa
     3601 accatgtgga attattgtgg aatcgttcgt acagtcgaag gattaaatca aggtttaaac
     3661 gtcatctacg aattcgaaaa agaaattaat aaaactcttg taaagaaagg tccaaagaat
     3721 attgattttc ttgccttccg aaacgcagtg caaaactcta agcttatttt aaagagcgct
     3781 ctttgccgaa aagaatcaag aggtcttcat tatattttag attatcctga tctagaccct
     3841 aaaacaaaac accatcgtat ttatcttgat gattaatttt tagaagagtt attatatttg
     3901 ccttatggca actcaatctt cttctggaaa aagcagaaaa aaatcaaaac ctgaaaaaat
     3961 tggtggttat tctccgactc aaaaaattgg acaaggagcc atgggagata tttggctttg
     4021 tcacgaccct tccttagatc gaatggttat tgtaaagcaa atgattccaa atttaactgc
     4081 ttatgaagag ttaaacttac gatttcaaag agaagcctcc cttttagcac atctaaaaca
     4141 cccaaacatt gtccacgctt attctctttg gcaggaacgc acagggcgtt tgtctttagc
     4201 aatggaattt attcatggaa agacactacg tgaaatttta gatgtctccc ctactcctcc
     4261 gctatttgcg actctttatt ttttacatga gattctacaa gtactagcct acgctcatca
     4321 aagaaagatt atccatcgtg atttaaagcc aagtaacatg atggtggata aaattggacg
     4381 aatcaagctt ttggattttg gaattgctcg aaacgctgga gcaactcaag acatgactct
     4441 tccaggaagc gttctcggca cagccgccta tatgagtcca gaacaaatta ctggaaaaaa
     4501 agtaacgctt cattctgatc tttttagcct tggaattatt gcttttgaaa tgctcacagg
     4561 gaaacaccct tttcgtggtg aaaccatgga actcaccact caatacattt taaatgtaaa
     4621 aattcacaaa aacttatttc caaaacaagt acctcatgcc ctgcgtcgct tcgtttgtaa
     4681 aatacttgaa aagaaaccct ctaaaagatt ccaatccgct caagaagcag cagatgaact
     4741 ttctaaaatt atggaaggat ttcctcgtca actagatgta tatatggggc aatggttaga
     4801 ttcattaagt catccagaat ccaaatttga accgccacct caaaagaagc acaagtctta
     4861 tttgtatcta gcagttggat ttattacagg gattattgta gctgcatcta cctccctatt
     4921 tatactaaat tgagccttta tgaattggat agacattact tgcttaatta tcgttacttt
     4981 gtttacattt cttggaatat gggctggttt atttaaaagt atttttaaac tcgttgcttg
     5041 gattattgcg gctattggag cctattatgg ttacgatatt gcggggccat ttatttcttc
     5101 gaattttgat ctaacacaaa ccacgattcg aatgatatgt gtaataggag gctttttaat
     5161 tcctctattt tcgatttata cttttatttt tattttagat cggtggatca aaaaaactcc
     5221 tttaaatgga ttgaatcgac tgggtggcgc tcttttcggc cttgctaaaa gtttaattat
     5281 ttgctttgta cttttaacaa ttattcacta tttaccattc tctggatctt tacatcaaac
     5341 aagaaattct tccattagtt acgaattgta taaagaatta gcggcaaaag atatttcgac
     5401 tcaaatttcc atgatccaaa caaaagccgc agaagaagtt aaaaaacttc aagaacaaaa
     5461 ccccgaaaaa aaagaatcct cttcttcaaa agaggatagt tcaaaatctc aacgaaaata
     5521 attgatataa aacttaaaac ttctttttaa ctgaatttac tgattcaaaa ataaatctta
     5581 atttttcgcc gcaaactttt ttactctctc taaaatttca atttcatttt caacagattt
     5641 attttccatt aaaatttgtc catgacaaat cactttgtct atacagcgtg cgtcagctgc
     5701 ataaacccaa tttgaaacca gatgatggtt gggaattagt ctttcattat taagatctaa
     5761 aagaattgca tcggccagtt tcccttcttc tataagcccc gcatttactt gataagctct
     5821 agaaccctgc accgtagcca tatgaaaaac ttcatctgcg ctaagtaatt caggtccacc
     5881 atccactttt gctagtaaag cagcaaactt catttcttct ctcatgtcta aattattatt
     5941 agaagaagct ccatctgtcc caagagcaat tggaatacct gccttcatta aagctgaaat
     6001 attcattttt ccactagaca atttcatatt agatgatgga ttatgaacaa gagtcacgtc
     6061 tcttttttga agaatatcta tatcttctgg agtcaaatga atcgcatgag cgagcaccac
     6121 attttgattc aaaaccccta aagaatctaa atactgaaca ggtgtttttc ctgttttttg
     6181 aatacagttt tctacttctt ctgttgtttc tgcaagatgc gtatgtaaaa caagccctaa
     6241 tctttgagcc gtttcagcac atcgcctcca taaaacgtca ccaacagtat aaacggcatg
     6301 cggcatgaca gcaagttgaa ttcgttcatg acaagccgca tttttttcta aaaaatcaaa
     6361 attatcttca atacttttcg gagacattaa attttcagca aaagtcactc caatagttgc
     6421 ccgaacaccc atctcaatta ccgcacgaat ggtttcttcc cgattccaat acatatcgga
     6481 gaaaaaaacg gtccctgact tgatcatttc taaaatggct aaacgagatc cccagtatac
     6541 atcttctcgt gttagttttg cttctaatgg ccaaatgtat tccgaaagcc atttaaaaag
     6601 actcatatca tcagcatacc ctcttaaaag agtcatcgca gcatgagtat gagcattgta
     6661 aaaaggagga atgattgctt tttctgcccc atcaatcact cgagtatttt ttggaacctc
     6721 atctaatgaa atgcttttag caattttttg aaaataatgt tcattgatga atatatccac
     6781 taaatcatgg tctttccaga cagactttat taataaaggg ggcacatcat actcctttaa
     6841 aagatgaaaa gaagcccttt ttgacataaa accttaataa aaagatgatt tatgcctatt
     6901 tgtacaaaaa aacacgtcca atacaaagag taaaaaagta taatatatat atgtttgaag
     6961 aaaatatttc caaatttccc atatctaaac aagaattttc tcgtttaaag gtttttcata
     7021 atgtctgtta tgaaagcgta attggttatt tacttcactc ttccattgat gaagtagaac
     7081 aaggatttgt tctgattgat ccttctaaaa cggacaaaaa actagtcgtt ttactggaag
     7141 ggaaacttga agttcacatt gattccatcg atggagtttt agcagattat attgaagaag
     7201 gtggttgcgc tggtgaaatg tcgattttcg acaattgtaa tccaagtgct tgggtgattt
     7261 ctagtacaaa atgtaaagtt ttgattatct cagcgccaac tactttagcg atgctgaatg
     7321 catcccatga tttatgcctt aattttttac atcttttaag tcaacgtgtg cgctatagca
     7381 ataaagtcgt atgtgaagat caatatcata ttcgttgcat tgaacaatat tcccaaatag
     7441 atactctcac aagtcttcat aatagacgtt ggcttgaaga gatgtacacc agagaactca
     7501 aaagaagcca tgctggtgat tttaggcttt gtgcttttat gattgatatt gactttttta
     7561 agagaatcaa tgatacatat ggtcatttag caggtgacgt ggtattggtg gaaattgctc
     7621 gcacattaaa cgaaagttta aggccttcag atatgcctgt tcgttttgga ggagaagaat
     7681 tttctgtgtt cttaccagga actacaagtg aaaacgcgaa agtcattgcc gaacgtatta
     7741 gagctaatat tgaaaaaaaa gaaattaaat taccctctgg agcaattgta catgtcactg
     7801 tgagtattgg ctttgctgaa agagaaaaat cggattctgt agaatcttta attggtaaag
     7861 cagataaagc tctgtaccaa gcaaaacagc acaatagaaa ccgaatttgt atgtacgtta
     7921 gtaaagacga aacgttttta ttttaagttt tccaacaatt cttttacata taaagcgaac
     7981 tcacctaaac tagggtctct cgctcccatc aacaagatgc aatctgaagg ttctgctaaa
     8041 cgaaccattt cttttgcaca ttcttttcta tctgcaaaat aatgggcttc tacaccttct
     8101 tccagtaaat catctactaa gtctgcactc gaaatatctt ttacaacggt gccaccagca
     8161 taataaatgt cactaaagaa aatacagtcg ttttgagaat ccgtatttga aacacgcaat
     8221 gcgcctttga tctcagagac taaatctttt cgtaaaaagc gagtgggccc gtaaccatgt
     8281 ggttgaaacc aagcgatcac acgaccttct gtaaaagctt ccacactacg taaactggcg
     8341 gcaatttttg caggattatg cgcaaaatca tcgactaagg taatccctga aaaagtgcct
     8401 aatatttgat gccgacggta aattccagaa aatgaagata atgcttcaga aatcgttttt
     8461 aaagaaaccc ctactcgaag agccactgct gtagcagcaa gcgcattttc catattatga
     8521 cggccaggaa aaggaagatc aaaacgaact aattcatttt ggtaccgcac tctaaaacgg
     8581 attcctgttc caatcgcaga aaaatcggtt ccttggacac caacatgatt ttcagaacca
     8641 aaatcaaact ctcgcccagc actaaattct tttgctctag gatgtgcatc atttacaatt
     8701 aaggttttgc ctcgatctac cacgtgatga gaaaaagtgg taaaaatttc gtttaattca
     8761 gtgagttcct tatgatcttt gtccaaattt aaaatcaagc ctatttctgg ctcatatcga
     8821 attaaagtac catcactttc atcggcttca accactaacc attcaccaaa acctgcaact
     8881 gcattaccga tcttcccttt tttttcaaga gaaactaaac cagctccagt aatcatagag
     8941 ggagaaaggc ccgcataatc taatacatga aaaatcatcg ccgttacagt acttttccca
     9001 ctcgtaccac ttacagcaat cgtcttaaca ctttttgaaa ttaaagcaag gatttcactt
     9061 ctatgcaaaa tttttagccc aagtgctttc gcttgaacaa tatcaggatt ggtatcttct
     9121 atagcagaac tcacgaccac tgcagaatag gttgcagaaa caccagaccc atcttgtaaa
     9181 aaacattgta ttcccatttc ctgcaattgt ttttgcacca agggaggcgt tgtagaagaa
     9241 aattgcctgt cagagccacc gatggaatgt cccttcccag ccaaatactg agcaatggca
     9301 ctcattccag aaccagctaa accaataaaa aaataagact cagataatgg aattgaactc
     9361 atctttaacc taataaattc ggatctattg ttggctcata gccaggaaca atgtattcag
     9421 aaggaatttc gccccttcga atggcttctc tttcttcttt tttacgacgt ttttcgattt
     9481 caaattcttt tctacgagca atacgttctt tcttcttaag taaacgttca gcacgcgtca
     9541 tgcgttcttt tacaggttct tcttcataaa gataaggatc ttcttcaacc acttcttcta
     9601 cgtcaatata acgcctatca ctttcgaaat ctttgaaaaa tgtctgatta aaacttggat
     9661 tatcacaagg agcttctttt cgaaggtact ttaaaacatc ttcaaaaggt ttttcaagtg
     9721 gtgcttcaaa agttaccatt ttgcctgtac ttggatgaat taaactcaat ttaacagctt
     9781 gtaataattg tgcagggctt ttttctaaag cttcgctggc gacatctctc actaaaggag
     9841 gaactcgcat aaggcaatta tctcgtccat catacaaagg gtctccgaaa actggatttc
     9901 ccatatagcg attatgcaca cgtatttgat gtgttcgtcc tgtttctaat tctaattcaa
     9961 gtaaagaagc aaaagtgaaa aactcaagaa ttttataatg agttctcgcc tctttaccgc
    10021 cccaaactat acccattttt aggcggtttt taggatcacg atcgatggga gcatcaatag
    10081 tcccttctaa atctcggggc tgtccccaaa ctaaagcttt atatgttcga tgtaaagtgc
    10141 gattttctag ttgagaagct aatgataaat gagctttatc atttttagca accaccatca
    10201 aaccaggagt atctttatca agacggtgaa caatacctgg acgtaaaggg ccattcactt
    10261 gagataaatt ttccttaaaa tgatataaca agccagcagc caaagtccct gtacgaacac
    10321 cacttccagg gtgaacgact aattgtcttg gtttattaat cacgacaata tcatcatctt
    10381 cataaataat atttaatgga atatcttctg gttctaaatt tgattcttct tttttaggga
    10441 gcgcaagcac ctgtatttcc atttcaggct ctaatctgaa gttctttgaa atcgtggatc
    10501 cattcacaag aactgaaccc gcagcgataa gcttttgtat atcggtacga gaaaaatcct
    10561 ttaattgagc ttgaaggaaa cgatcgattc gctcttcagc ttgagactca cctactttaa
    10621 aacaaatcat ttttttattt ccttctccaa tttaatttgc tgatgaatct ttttcagtaa
    10681 tactggagat agaaaaacga gaatcacacc aacagtcaca aagctatccg ccacgttaaa
    10741 tgtcgggaac cgcgccataa taaaatctgg gaaatcacaa tcaataaaat caaccacttt
    10801 ttgcaatctc attcgatcga taaagtttcc taatgcccca gagataatca taatcacccc
    10861 taaacgggaa acccaatctc gataatcaat agtacgaaag aaccatatta aaaccgatag
    10921 cgcaatgata gacatcaaca aaaagaatac aacaggaggt aaaaaaggca tgatatcttg
    10981 aggacgagaa ctaaacgccg ccccttcatt atacactaat tgaaaacgta acaacggttc
    11041 attggcgatg gtaatgattg gaatcatttc acgagtaagt gtcccatcct cgtttgtaaa
    11101 cctcagaaca gcccatattt ttgttatttg gtcagcaatc agcccaaaaa tacctaagcc
    11161 tatttgaaac tttaaggttt ttatcttgtt aatccaattg ttgttcatgg gcgtaacctt
    11221 gaatattcct tttcaatcca acgatttgaa taaaaatgaa gcgatgtgtc tttaataatc
    11281 tttttaacag tactacgaga aatctttgct ttttcttcag aatcaaaaat aattttaccg
    11341 tccccaatca aagacaagtt ttctgctgcc ataaaaagag gccacaaaca aaaaagacgt
    11401 atacgacgat ctacacgagg aatctttaat acataacgaa tcgaatcatc taaatgagac
    11461 cacgccttat ccactaattc tttcataata gcagaacgta catctggact cacatcaggt
    11521 tcaaaaatat cataagaatt ttttaaccca tgacgccaac aaatttcttc tgggataaaa
    11581 catacttttc gagaagcatc ttctcgaaca tctttaataa tattcgtaac ttgaagagct
    11641 aagccaaaac tcacatccca cttttttaag tcatcaaaag tttctacatc aataaaacga
    11701 ctgtcaataa agaataattg ggatagcatt tttccaacaa tacctgccac ataataacaa
    11761 tactgatcta attcagaagt tttctttatg gtaaaccaac cttgagccag ctgctgttct
    11821 tgttttaagg caaattttgc cattccttgg cacatttcta taatcacttt ctgaataatc
    11881 tttctattcc ctaatggaag ttcgctccat aagccaataa ccactggtgt tcgacaagtc
    11941 aataaatgat taggatcttt agattgaatc cactgagacg ggagcatact caaaaagaga
    12001 tccactcttt taggatctac ttctggagtt tcaaaaatag aggcaaaata ctttaataat
    12061 ttttcttttt ctgtggcagc aagatcagga tcatcttcaa tagtatctgc aatgcgtaag
    12121 aataaataag caagcaatac tgattggtgc aaaaggccag ataagacatt gatgttaaga
    12181 gcaaaagtac gagagacttg taatagaata tttttagcga aacaaaaatc aggagaattt
    12241 tttttaatca tgaaacatag cctctgcgta taaatcctta ggtttttttt gcgattttga
    12301 catagatact atgtattgta agaaacgccc atgttcatca gctctcttca aatgactttg
    12361 aaaagaccaa gtcgttcgat tacgaatatc tgtatcttgt atttttttcc cctctttttg
    12421 atccttaaaa acttgcttgc gatattcaaa gaaataacac tcttgctcta aattacctac
    12481 atatcgaata gaccagcgag caaagaaaac ggctagaata gtaaaaaaag ctaagccaac
    12541 aaagaacacc caagaactaa gcggaataag ccaataaagg gatgaggcgc taaaaaaaat
    12601 ggttagaaga agtaataata caccaacaaa tttaatttgc atatgttttt tttgattatt
    12661 cttccaccac aaaggaatat aaacccagtt tttaccagta cgtagcatct ttttgaggta
    12721 aagaggataa gctctataac gaaaccaaag gaaaatacct gcccaaaaaa gcaaaccaag
    12781 ccaaaagtac tgaccaaata tttttatcca agaaatcatg atccaataat actaaattgt
    12841 agggaaaata cttgaatatt tgtttaattt aaaagcgttc taatcacttc ttagagcgag
    12901 aggcactaga aaatgcagac ccaatgggaa aaaagcttaa atgtattacg taataaaata
    12961 tctagctcta tttttataac tctattttcc aaattaaaaa ttgcttctct taataacaat
    13021 cacgcaacga ttattgcccc ttctgactta gatcttaaag aaatcgcccc ctataagctt
    13081 cttacagaaa tcagctggga agaggctaat gggaaacatg ttgacttaga ttttcgacat
    13141 gcttccgtac tggtagaacc ttcttcttat agcaatagtt caattaaaga atttactcca
    13201 agtgtaccct taaatagcat ttatcgattt gaaaatttta ttcctggaga taaagcccaa
    13261 ttggccttta atgcagcctt tgctgttgca gaaaacccag atggaaataa gtataatcct
    13321 ctatttattt atggagcctc ggggctagga aagactcatt tattgcaagc cataggaaat
    13381 tacgtgttag aaagcgataa tcctaaaaga gtacgctatt taacagccta tgactttcaa
    13441 cagcagtata taaaaagcgt tagagaccaa cgcattactg aaatgtctgc cttctaccgc
    13501 aatgaagtcg atgtcttatt aatcgatgac attcaaaact ggagcggaaa agatgaaact
    13561 caaaatgagt tttttcatat tttcaacgct ctgcaccaag cagggaaaca aattgttctt
    13621 acctctgatg caccagcggt cgaggtcaaa agtctttcag atcgtcttgt gagccgtttt
    13681 tcatggggcc tcaccgtgga tatccaacct cccaatttgg aaacgagaga agccatcctc
    13741 agaaaaaaag ccgaggaaaa gcatattgat attgatgacg aagtcatttt gtatctcgca
    13801 gaaaatatcg aaagtaatgt acgattctta gaaagtgcag tcattaagtt aacggttcaa
    13861 gcaagcatca tgcaccacga catagacatc cacctcgctc gaaaagtagt tgcagatatt
    13921 gtcccgacaa tacgtcgaag agttagcatg gattctattg taaatgccgt ttcaagacat
    13981 ttcgaagttc ctgaagaaaa gcttctggct cctggtcgtg gtacgaaaga aattgctcat
    14041 gcaagacaag tcgccatgtt tttaatgaaa gaattaacaa cactgagtct acaaagcatt
    14101 ggaactcgtt ttggcggtaa agaccattcc acggtagttc atgcgatcaa aacgattaga
    14161 aaagaaatgg aagccactcc agcctttgaa agaacaattg aaaatttaaa agctgaaata
    14221 cacgattaag ctactttgac aaaaacgatc aaaaatgttc taccacttgg ctagagcatt
    14281 tgtaataata aggtcttgta atttttcaat cgctcttgtt tgcccgtgac gatcttccgt
    14341 ttcatttgct tgcacatcgt aaatgccatc tgcagaaaga ttcttttctt cataaatcac
    14401 acgatctttc acgttgtcat aaaaaagaac actcacacga atcgtgacac gataagtttc
    14461 cacttgatct gagctattat aattatctgg cttattggaa tagcttaaaa gagaaatctt
    14521 aaaatctgca gaagcttcgt cattaacgat gcgtacagat cctgcatttt ttcgaaacaa
    14581 ttctaaaata cctgttcgta aattattagc aagaacagga tctaacgttt tatcatccac
    14641 ttcaaaaata tgaaccgttt tgatatggct tggtaaagta cttgcagtaa aactataaca
    14701 acccgatagg ataaacaaaa agcctatgat aattatagac gaaccaataa aaccgactct
    14761 acggcaagta tttggagaga gtccaacaaa agaaagaaat ctgaataaca cttggtaacg
    14821 atttgtcata atgaaagaac ccctaaaaag gagtgaaaac agtgttgaga atacccattc
    14881 aataaaagag atattcccgt atttttgtaa tattaactaa attttgattg atttttttac
    14941 caaaagaggc ttctccatgc ttgatattcg taaaattcgt gaaaatcctg aattctatca
    15001 cattgaaaca caaaaaaaag gttcctctat tgatctaatg agcgtccttg cgatcgatga
    15061 aaaacgtcga acactactca ctgaagtcga aaaattaaaa agcgaacgca acgccgaatc
    15121 caaacgaatt ggagaagcaa aaaaaagagg tgaaaatgca gatgaagctg ttcaagcgat
    15181 gcgagttctt ggagataaaa ttgctgcatt agataacgaa atgaaagaat tggataatga
    15241 gcaaactgaa aagttaatgc acattccaaa cgtacctcac tcctccactc ctgaaggcaa
    15301 agacagtagc gacaacgtcg ttcaatcttc ttggggtact ccaaaagagc attcttttaa
    15361 tgccgttgac cataaaactc ttggcgaaaa attaggccta tttgattttg aacgtggggc
    15421 caaaatttct ggcagtggtt ttcctgttta tcgtggcctt ggcgctagac ttgaacgggc
    15481 tttaatccaa tggttcttag attctcatcg agaagctggt tttgaagaaa tcattcctcc
    15541 ttatttagtc actcgaaaca ccatgcgtgg cacaggacaa cttccaaaat ttgaagaaga
    15601 catgtatcgt tgcgataaag acgatgatct tttcttaata ccaacagcag aagtacctgt
    15661 caccaatctt tatgctgacg aagtgattcc tgaagcagaa cttccaaaac gtctctgtgc
    15721 ttattctgct tgctttagaa gagaagctgg tagttacggt aaagacactc gtggtttact
    15781 ccgccttcat cagtttaata aagtcgagat ggtttacttt gctcatcctg aaaagagcta
    15841 cgaagagcat gaaaacctaa cccgctttgg agaaattcta cttgaaaagc taggccttcc
    15901 ttatcgtcgc cttttgcttt gtaaaggaga cttaggtttt ggtgctgcca aatgttatga
    15961 tcttgaagtt tatgctcctg tcgaaaagaa atggctcgaa gtcagctcct gctcgaattt
    16021 tgaagacttc caagcacgta gagcaaatat cagaacaaag attaacggga aaaacacatt
    16081 ccttcacacc ttaaacggat ctggtcttgc cactcctcga atcatggttg gaatttgtga
    16141 taattaccaa caacccgatg gatctttagt cattcctgaa gtgctacgcc cgtatatggg
    16201 tggtctagaa aaaattgaac cttccctata aacccaaata gacagaaata agatgacaaa
    16261 aaaatttgat actccaatta cagaagtccc tctttttcac caaggaaaag tgagagacat
    16321 gtatgatctc ggcgattctt ttttaatggt cgctagtgat cgtctttcgg cttttgatgt
    16381 tgtattacct actcctatcc caggcaaagg caagatatta aaccaacttt cattgttctg
    16441 gtttaaactc ttggatatgc caaatcactt gattacagcg aatgtagacg agtacccaga
    16501 agttctaaaa aaacatgcgg attaccttcg cggtcgttcg atgattgtga aaaaagcaca
    16561 tcgccatagc gtcgaatgca ttgttcgtgg ctatatcgta ggttctggtt ggaaagatta
    16621 tcaaaagact ggaaaaatct gtggacatca attgccacct aatttgcaat tatgccaaaa
    16681 actagaacaa gcgctttaca ccccaagtac aaagccagat gtcggccacg atgaaaacat
    16741 ttcttacgaa gaaacatttg atatcgtcgg agaaaaagtc gctaaaacat taaaagacct
    16801 ctccttagat atttatacta aagctcgtga ctatgcggct agcaaaggca ttattcttgc
    16861 cgatactaaa tttgagtttg gagaaattga tggcaaaacg attttaatcg atgaagttct
    16921 cactccagat tccagccgtt attggcctgc tgataaatac gaaatcggaa aaaatcaaga
    16981 atcttttgat aagcaatata tccgtgactg gcttgaaact ctagattggg ataaaacaga
    17041 tcctggtcca gaaattcctc cagagattgt taaaaagact ctcgaaaagt acattgagat
    17101 ctttgtacgt ctcacaggaa aagatcctgt tttataagat cattagctta atacgcttac
    17161 tataagcgtc caaatttata aaaagactaa tacttgatat tagtcttttt ttatgctttc
    17221 aaaatcaatg tattgctcta aatttcaaag cagccatcta ttaaacaaat gaatgatttc
    17281 atctgattac attacgcaaa tttatttaaa aacacttttt tataaacctt cttttataag
    17341 atttcctttt gtatacactt tttgaaaatg actttaaaac ggatttcttt ttagaatata
    17401 ttttgaatct atgaataaaa taaagcttgg tatattaggc actggctaca tcgccacaaa
    17461 aatggctgag gccgtaaaat atctagaaaa tcactctatg ggaatagagg cctatgcagt
    17521 cgcctctcga aataaaagta aagcagatca atttgttcaa gaacatcgat ttcaaaaagc
    17581 ttatggctct tacgaagacc tagtaaacga tcctatcgtc catcttattt atatcgcgac
    17641 acctcattcc gaacactttg aaaacgcgaa actttgcata gaaaaaggga aagctgtttt
    17701 agttgagaaa gcgtttacat ccaacgccaa gcaaggatcc atactcattt ctcttgccga
    17761 agaaaaaggc gtccttttag tagaagccat gtggactcgt tttttaccag ccataaaaat
    17821 cattaaagac tggattactg caggtcgtat tggcgaagta aaattaattg aagccgattt
    17881 ttctcaaccg atttcacaca tagaaaggct acaaaatcca agtctcgctg gcggagccct
    17941 tttagattta ggaatttatt gtttaacgtt tgccgatcta ttcttagaag gaaatattat
    18001 aagcacccaa agccactgca ttccttttaa aacaggtgtt gatgcaagtg attggattac
    18061 cctcacttat tccagtgggc aaaaggcatt cttaaaaacc tcgatggtca cgcctcctaa
    18121 aaacgaaggg attctctatg gttccaaagg ttatattcgc gttgaaaact taaacaatat
    18181 ggttcgattg gaactattgg atcacacttc tcaattatta gaaacgattg ttccaccaat
    18241 gcttatgaat ggctacgagt atcaattact cgcttgtaaa aaagcactgg aagcaaaacc
    18301 tcgtatagaa ggcaaccgaa agatttggga atgcgaagaa atgcctcaca gcaaaacgct
    18361 ttctatgatg gaacagctcg attctcttcg tgattctttt ggtgtaactt atccttttga
    18421 aataccaaac tcaaaaatct gggatcgttc taaaaacaaa tctattcttc aaatttataa
    18481 tattgagact aaagaatgca tccgcttaaa agagtttgat tacgttattg aagccccaaa
    18541 ctggtctact aacggagact ttttaaccta caatagtaat ggtaaaattt tcaaatacga
    18601 tattacaaca gctacaataa cagaagtccc ctcctactat atagatcaat gcaataacga
    18661 tcatgtatta gctcctgatg gttctggtct ctacgtaagc caccacacta aagaagacgg
    18721 tctttctaga atttatcgta tattctttga tggtcgtaag cctatactca tcacgccact
    18781 aggcccaagt tacttgcacg gagtctcacc tgatgggaaa accttggctt attgcgccgc
    18841 acgtaacgga gaatacgata tttatacaat accaagtgaa ggcggtgatg aaacacaact
    18901 gacttctact aaaggcttaa atgatggccc tgaatatgat tctactggag aatacatctg
    18961 gtttaattca gtacgaacag ggcgaatgca agcctatcga atgaaagcaa atggagaaga
    19021 acaaacacaa atcacagagg atctccattg gaatacttgg ttcccacata tttctcctga
    19081 taaaaaacat gcagtgatga ttgcttatca tgaaaatgac gtaaaaccag gtgaacacgt
    19141 tcctaataaa aacgtagaaa tccggttgat taaagtagaa ggcaaccagt ggagttctcc
    19201 cgaaacaatt ctcaaactct ttggcggtca aggcaccatc aacgttaatt cctgggctcc
    19261 tgacagcaaa agatttgctt tcgttagtta cctttaagaa accattttag aagagctact
    19321 tgaactactt gagccactta gaatgtcatt gatttttgag gaactactat tgtttaagtc
    19381 gtttttttca ccttttgaag gattactttc tccacacgct atcaggatta gagtgacaaa
    19441 aatcaacgta aaatgcaata acttttctga ttacataaaa acccccttta atttgtaaga
    19501 aaattataac attcaaagca tacaaagttc gataaatata tatttctatt cagattatca
    19561 taggagcatg gctatgtcaa tattcgtacg aaaacactgg agaaaaataa tcctagcttt
    19621 aactaccgca ttttggtcta gt
//
LOCUS       DAOOBB010000011        19356 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_3767_length_19356_cov_5.576291, whole genome shotgun sequence.
ACCESSION   DAOOBB010000011 DAOOBB010000000
VERSION     DAOOBB010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 19356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19356
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3767_length_19356_cov_5.576291"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="PLT31_01155"
     CDS             <1..846
                     /locus_tag="PLT31_01155"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93770.1"
                     /translation="EQLEEDIQKCGGKLKETRCVVEGADGWLFLQESLINIVADWHPN
                     TEQIIAFNDSLEERGIELVVVPVPDKLQAEADHYINIPNYEITARNYDDWIETLQKND
                     VTVINALDKFRELRNYTPMFEPNESHCTGAARQVLAEMVADSIEDLFEDNPKKQYLLN
                     DTLVPGTGNLYRLLHKKYSNYKVEEAKVITPDGKKYRGSKKAPIVVIGDSNAGQGSNI
                     SSNIGAYIAQSIGEETYTISKVGGGNIGPTLFKNRDEFLHGKRAVVWVFDGRELYGEF
                     EKPAF"
     gene            1007..2080
                     /locus_tag="PLT31_01160"
     CDS             1007..2080
                     /locus_tag="PLT31_01160"
                     /inference="COORDINATES: protein motif:HMM:NF014715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HPW93771.1"
                     /translation="MEIKWLNPDSPFDRLILAGDIGGTNTNLGLVGYKKGKFTLILET
                     VFPSATLTSLAEPLAETLKIAKEHSPELKPEYCCISAAGPVSNNRCVMTNLSWDIDGN
                     ALTTIIGIPVMIINDFVAISYGIPTLDVEDPTQIHKLAHSDGSYAEPQPATKAVIGPG
                     TGMGVSFLAFDGKRHIPASSEGGHMSFSPFDEDSRSFCDYMTKKLGTQPGVEVFVSGL
                     GISNMYEWWKATKGIPSNPLFQKIEETEPSDRPKYISRASDTDPVAAEMMRLFVKMLG
                     RFASDVSALLLPLGGLYLAGGTVQKDMRWLERDHLFMKYFEKNYNPNIRPLLQKMPVY
                     IIKDYSISLYGAANASLNLQQTK"
     gene            2341..3966
                     /locus_tag="PLT31_01165"
     CDS             2341..3966
                     /locus_tag="PLT31_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPW93772.1"
                     /translation="MPSFDPSAKKMLLSGNEAISLSMLHCNVQIAAGYPGTPSSEILE
                     TYSEIGGYAQWAPNEKVAAEVALGASFGHARSVVTMKHVGLNVASDVLYTATYSGTQG
                     GMVWIVADDPGQASSQNEQDTRNHAKAAVCPMFEPSNSQEAYDMVRLAFQTSERFGIP
                     VILRMTTRVDHSKSIVQVSDEKLEALKPHFVRDIASRVMVPGYSKPAAKRLRAKMEEM
                     AAWNSAEGPNRMEMRSTDLGIITSGISYHHVREAAPEASVLKIGMSYPLPVNLIREFA
                     AKVGRLVVIEENDPWLFEGISALGIKVEGKYDPIYRFGELDVNRVRRILARDNSPEPV
                     PVKGRAPALCQGCPHRESFAVLNELNCIVSGDIGCYTLAALPPLSAMDMMIDMGASIG
                     MGLGLRHVLPREEAKRVVSVIGDSTFIHSGITGLVEMGYNRPSTGHVVIILDNSITAM
                     TGQQEHPGTGRKLDHSPAYKVNYGEIAKAAGIENVFEVNPVKEYDKMKALVKEALEKD
                     ELTLIIAKSPCILALKSILAWDKANREKAGDQK"
     gene            3963..4430
                     /locus_tag="PLT31_01170"
     CDS             3963..4430
                     /locus_tag="PLT31_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPW93773.1"
                     /translation="MSTMNVKFAGLGGTGVVKASDVFAELVFELGYDVKKAEVHGMSQ
                     RGGSVASDVRFGEKVESPMIPDGEAQFLVVFAEDQIPVNEAALKVGGTLLSPKDIDVS
                     KLPTEKALNVAMLGKLSTFFDFPIEKWEKILQKLFAPKFHEGNSIAFKIGREG"
     gene            4740..7004
                     /locus_tag="PLT31_01175"
     CDS             4740..7004
                     /locus_tag="PLT31_01175"
                     /inference="COORDINATES: protein motif:HMM:NF039970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6055 domain-containing protein"
                     /protein_id="HPW93774.1"
                     /translation="MINTKVGFKITLLLLLMTSFLFAAKEIYLPNFFKQQGIDFESPN
                     AQWSRSRMDSTANWIILWESGFGSDPSSNAAPYKVNMSQLKEVLEKSYEMNLNTMKMV
                     VKGSSKTDQYKMIVFIKYSTTWGAYGSGYDNVIGSLEVNPEAANIPSVVAHEIGHTFQ
                     YQVGCDVPEGGFRYGLGANGEGGNGFWEQGAQWASFQVYPEEQFLTYDFNTYIKSNHL
                     HILHEEPRYANYFLPDYWAYKRGIDFMGRLWRESRRPEDPVDAYKRLFNVSQSEFNEE
                     IFEHAVRLTTWDLPAIKERGASYIQRRAQVKMKKTTDQYWLVDSAVTIENYGYNSIKL
                     NAPSTETQVSVYFQGKAGASGYRSINIDKAGWYFGFVALLNNGERVYSDVGSLEYSGG
                     KNPEGALSFNVPAGCSYLWLVVSGAPQEHWHHVWDDSTYNDEQWPYQVKFSNTNLLGE
                     QNESTTNYSLSTAVDGSGSISPSSGSYPSGSLTLKATPTPGYVFDHWSGDLTGSENPA
                     VLKLDQDKSVTAHFIKLKTTVEEFTFQLTMSPKSDYSPTAVPLDKSAIALAFGLSESD
                     LVEKIGTDIRYYAVNPDESFDANSTAIDPGHWYNAEGQVVSYGDQAVIYSEINKNTLT
                     ANIGQYPDRSVKGDSYLIKQALVYQKSATEEVQVHLIYSIKIDESLKIRTRALPPILS
                     QGERPILSSEILSLMSAGRGELQVYNLKGKLMASVKQVSSWSDLGMNQNGVYKIVFLE
                     NNKIKAIQSISYVR"
     gene            7145..8008
                     /gene="lptC"
                     /locus_tag="PLT31_01180"
     CDS             7145..8008
                     /gene="lptC"
                     /locus_tag="PLT31_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HPW93775.1"
                     /translation="MACFLHRTLNKTLKNLQWILIGGISFLCAGLFSGCEKIEEDAPW
                     INVERPLMLFNDTTLLDFYEEEVLSWKLKTAYLERWASNDKVFARPILVDIFDSLGEK
                     VAFLRADSGRLDMGMNYVFAYGHVFAFTPKGASVRADSLLWHKRDNMVKTDSYVRVVT
                     EDGDVLQGKGFISDAKMDNWRILSEVTGIFQDAAKRLKEEDKTLLEETSSPSSTREKP
                     STKNEKQESVSNNPRMNKAKKTDREVRVLTDKEVAEDRQKRSMLKRDSLRKERGIDPK
                     SKRPRARRMAE"
     gene            8005..9297
                     /locus_tag="PLT31_01185"
     CDS             8005..9297
                     /locus_tag="PLT31_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93776.1"
                     /translation="MSRYHFSILFLLLFCSMLCAQSSPLIMRHADSLSVARTRGSLLL
                     QGKVHFIHDSIQFKTQRAIWNRDIDMVQCDGGFLFTHTDGFIRASSGVYQKKNNFASA
                     TGNVVAADSQNTYAFFGEHLTYDRKEGILTMPDKPILHQYEMKGDSIDTLSIKAKKIV
                     YHKKNEFASAFREVKIQQEKMLVTCDTAYFDKKNNWMSLSGNPICHLENEMIQGDSIY
                     LVLEPDGKSLKSALVIRNAHGKQVQKGKGRQPAQHTEAFGDTLFVEFEKGKIKHLYVN
                     VNAKGFFFEEDLPDYKNLMDGNRLDIYFEKGKVNKAIVGGNAQSTYFYVKENRSVSGR
                     NESSGDTIQVDFKDGKVGSLRMIGGKTLASGRYFDLEKSVGKEDKRKEFKRDSTIVRK
                     PESERRRNRMERIKKNQVILSKDSLRARKNLEGKKSDE"
     gene            9290..10027
                     /gene="lptB"
                     /locus_tag="PLT31_01190"
     CDS             9290..10027
                     /gene="lptB"
                     /locus_tag="PLT31_01190"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HPW93777.1"
                     /translation="MNDVIHTIHTNKLCKVYSGKQVVHEASIRVSQGEIVGLLGPNGA
                     GKTTTFYMIVGMIRPDSGQIFLDDIEMTSKPMYRRARLGVGYLPQEASIFRKMNVEEN
                     IMAILETQNLKRVERKNRLEELLSEFKIDHIRKTKAYSCSGGERRRLEIARALASNPK
                     FLLLDEPFAGIDPIAVADIQSIISDLRDKGMGILITDHNVRETLSITDRAYIMYKSQV
                     LTEGSSEFLAGDEEARRIYLGESFRLN"
     gene            10037..11614
                     /gene="rpoN"
                     /locus_tag="PLT31_01195"
     CDS             10037..11614
                     /gene="rpoN"
                     /locus_tag="PLT31_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HPW93778.1"
                     /translation="MNLGMQLNQGVRLEQTLSPQMLQSITILQMNTLDLETAIKQETE
                     QNPLLELADTEEKDEADSLVEDYPEDYSDVNSDSDDVETGLIDDPDEKLIDWDKYFEE
                     GFVDADRPLKDLNVPDPEEEEWIRQPTESQSMQDFLKNQLRSWKRPERIVVVVEYLID
                     CLDEKGYVQSALAQDVSTLQVRSGFKEVEEISPEYLASSNPDILEAEEVLQNKKELEE
                     ASFFVREAFHILQSLSPRGIGARNLRECLLIQAYSLPDFSLLAIRILEKHFESLKTLR
                     YGVIAKELDVTTEEVQGAVQELSKLSPHPGVQISNSSIQMIVPDMSVVEEKPGVFKVI
                     VKEAPFRSRLRINQTYRKLLMNSSTSKKDKEYIREKLNAANSFLRSVDNRQSTIELVM
                     NAILKRQMDFFIKGPEYLHPMILQTIADDIQRDPSTVNRVTNGKYVETPYGIYELKQF
                     FTNGLKQDDGSDLSSARILNAIKELIENEDRKKPLSDQALTNALEEKGIRIARRTVAK
                     YREEELKILPARLRKEV"
     gene            11841..12164
                     /gene="raiA"
                     /locus_tag="PLT31_01200"
     CDS             11841..12164
                     /gene="raiA"
                     /locus_tag="PLT31_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HPW93779.1"
                     /translation="MDISISARHFNASASLQARVQEEIEKLGKFYPNITSATIVLDHE
                     VEHMRRCEISLNITGNVVVASADEENMGKALDVALERAKTQLKKQNEKQKDHKTQSLS
                     DLVNK"
     gene            12161..13189
                     /gene="hprK"
                     /locus_tag="PLT31_01205"
     CDS             12161..13189
                     /gene="hprK"
                     /locus_tag="PLT31_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HPW93780.1"
                     /translation="MSAYSLKDIHVNCREKYPVKNLWLKHKDFLRLELCTGEDSLNGF
                     IEESGLHRPGLAMAGFTSVYSSHQIQVVGHTEWNYLESLGSEKRREIFSNLSQFHSPL
                     WVITHEQKPHQELLDMCNAQGTPIFCTPLYTFEFANIVQRILEDFFAPYAVIHASLVD
                     VYGVGMLYIGESNVGKSECVLDLVERGHRLVADDVVHISHIGNSIIGRANPMVKHHME
                     IRGVGIIDIRSMFGIHAVRKTKKIEVIVELQQWDRHTTYDRTGLSSSLEEIMGVKIPH
                     ILIPVLPGKNLTVISEVIAMNSLMKMDGMDTASSFNELLLRRIKEKAKGNKDDYMDDS
                     IFNGNAYE"
     gene            13182..14063
                     /locus_tag="PLT31_01210"
     CDS             13182..14063
                     /locus_tag="PLT31_01210"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPW93781.1"
                     /translation="MNRFVYLFKNNFLAVLVFLLLAAIAFFAWFFFHPSSPYHERQTF
                     VIYFEKIGSLASGSLVRVNGMEKGKVLSTELTEDGVYVKVCLFADYNVPKNSSFRLIN
                     TGLVGKQEVSILAGDASGFLISGDTVNGLTDQGFSEISRNLFLILNELDTIKVTISSF
                     IDTITNGSIGKSLYSVEKKTRFLTQDTKNKVTSWITELRKVKEAALSIIPEDPKIKKD
                     IDLLLGDLNQLEKELEQLQQELASISFEIPEKGTVGQILKDSNPFLKKIDSLKVNIDL
                     LIEEIKKKGLALNIDIF"
     gene            14177..14653
                     /locus_tag="PLT31_01215"
     CDS             14177..14653
                     /locus_tag="PLT31_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HPW93782.1"
                     /translation="MTEKIPVKMDQKDLDFFEALLAERRRELVSAQMDSENTNVFHSQ
                     KDQGGELAGYSNHLADAASDYTSLETNFDLAAREGKYLVYLEEAIQRVKNGTFGVCKV
                     CKNLIPKARLEAVPTATKCVDCKEETKRKEREDNRIEMARLFAEQQRRELKTKTNN"
     gene            complement(14650..15693)
                     /locus_tag="PLT31_01220"
     CDS             complement(14650..15693)
                     /locus_tag="PLT31_01220"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPW93783.1"
                     /translation="MNSLKEKISLALSQAQSTQIFDKAVVGVLLPNQETLFISLNACP
                     DSVFDIASLTKVCPTSTIALHYILKGELSPETPVASWIQELQTNYRDQILLKHLLTHS
                     LDYRIPMSSLKHLSPEEILNHLYNYSFEKAPGTIFNYGNPASILLGILLQRFSGKSLS
                     VLGKELLFDPLHMTRSGWFPLQRMSRSEIIPTEHCAFRKEIIQGVVHDESAFILEQLF
                     PVGSAGMFSTIEDLMKFVQMLLQDGLYKGEQVMPAGILNIISRNALDPSMNAQTALGF
                     ELNNPRFMGTRCSSRTFGKTGFTGASIVADPEQNAAIILLSNFTWPHREEKPERIYTF
                     RSYLSDLFFESLN"
     gene            15913..16446
                     /locus_tag="PLT31_01225"
     CDS             15913..16446
                     /locus_tag="PLT31_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPW93784.1"
                     /translation="MEEYIDILNAHGEPLNYSASRTEVHAKGLWHRTVHIWVFDLNGK
                     ILFQKRSLKKENHPGLLDTSCAGHISAGDDSRNAGVRELAEELGIIKKTDDLKFLFES
                     KHETVLNNGSYLDNEYYDVYRVDLSLKEVEMLSPQKSELESLHWFSIKEFQDLLKKEP
                     SLFVEHPEDFKYLKRLG"
     gene            complement(16587..17984)
                     /locus_tag="PLT31_01230"
     CDS             complement(16587..17984)
                     /locus_tag="PLT31_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93785.1"
                     /translation="MKKIILIAFCSILLFGCANKSNVRYDALSQSLKQGGFTGAIQTI
                     EKEKEDLYGEKSAFLYHFDLGVLFHYNRDFKKSIEHLSKAEQVYDDLYAKSVTNEAAA
                     LLTNDNTRPYRARPFELLFLYQVQILNYLAINDLDGANVEVKRAQMAMEALYQKDSEK
                     VNDNGFLQYLTALVYEMNGEIDNAAISYYKAAKAYKEYGQKLPVELVDFMSYTLQKTG
                     REEDLKLLKMNNIPETPLSQSLEEKGAEIIVIGYAGHSPILGEMRMSGTFVNGGGLNL
                     TYTDAQTGEIKSFTMIAPIIAESGTATAHIAFSLPEKQVLPFDVYDFSIYLNDEPEIF
                     SENVTDLNDEITRNLKDDWSGTVAKTAARVALRTFAAQKTKKAVETNNPLLNLATSIG
                     TDVASSQLEKADLRVGLFMPNRIHMTRIPVGPGQHDLDIAALDSRGATVSRFTFKQVS
                     IKKGQKLFLFIPAIK"
     gene            complement(18006..18608)
                     /locus_tag="PLT31_01235"
     CDS             complement(18006..18608)
                     /locus_tag="PLT31_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HPW93786.1"
                     /translation="MNNFFRVLFFFSLLFLVACSSNQKVSRIDPNAVVDLSGRWNDTD
                     SRLVADEMIKDCLSRPWYSNMIQQKGANPTVVIGKVRNKSHEHINIETFIKDMERALI
                     NSGTVDFVASAPERQELRNEIESQAGNATDETRKDAGQEIGADLMLTGSLNSIIDQDG
                     GEQVIFYQIDLELTDLQSHRKVWIGNKKIKKYISRDAVKL"
     gene            complement(18714..>19356)
                     /locus_tag="PLT31_01240"
     CDS             complement(18714..>19356)
                     /locus_tag="PLT31_01240"
                     /inference="COORDINATES: protein motif:HMM:NF021347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-ATPase domain-containing protein"
                     /protein_id="HPW93787.1"
                     /translation="LIGSEREPLIPLIDRIVTLKKHGLNFLLVAGACGDYLQVADQVI
                     LVNNYLPENATAKAKLINEQNPPLSKLAEEENVVLPLHFSESRSFLTYIQPLLPHVRP
                     SSPVERQVKIKMKGDTCLQIGFLLSETNKIAGFCNTSQRFGAGYILLNLLQNATEEPS
                     FELIQKWCAQIQNVGFRKLPQGFSRDLSLPRTQEIAATLLRLRHYSKEQSSAK"
BASE COUNT         6242 a         3192 c         4030 g         5892 t
ORIGIN      
        1 gagcaattag aagaagatat ccaaaaatgt ggtggcaaac tgaaagaaac tcgctgcgtc
       61 gtggaaggtg ctgatggttg gcttttttta caagaaagcc tgatcaacat agtcgcagat
      121 tggcacccca atacagagca aattattgct ttcaatgact ctttagaaga acgcggaata
      181 gagctagttg tcgttcctgt tccagataag ttgcaagcag aagctgatca ttatatcaat
      241 attccaaact acgaaattac agctcgaaat tacgatgatt ggattgagac tttacaaaaa
      301 aacgatgtga cagtaatcaa cgctctcgat aagttccgcg aacttagaaa ttatactccg
      361 atgtttgaac caaatgaaag tcattgtaca ggagccgcaa ggcaagttct agctgaaatg
      421 gttgctgatt ctattgagga tctttttgaa gataatccta agaagcagta cctgcttaat
      481 gatacgcttg ttcctggaac gggaaacttg tatcgtttac ttcacaaaaa atactctaac
      541 tacaaggtcg aagaagcaaa agtgataacg cctgatggga aaaaatatcg tggttcaaaa
      601 aaagctccca ttgtggtcat tggcgacagc aacgctggtc agggaagtaa tatttcctca
      661 aatattggag catatattgc ccaaagtata ggtgaagaaa cgtataccat aagcaaggta
      721 ggtggcggca atattggtcc aacgttattc aaaaatcgcg atgagttttt gcatgggaaa
      781 agggccgtgg tctgggtttt cgatggtcgc gaactctacg gagaatttga aaaaccagct
      841 ttttgagtca gtaatcaaaa attcaagcgt tatttaaaga ctcgctcatt agattttttt
      901 tgtagagatg aattaaagta ggaatacata ccttaaatag gggttagtat acgccttttt
      961 aaaatcgaaa atgatatatt tagagcaatc atagtggagt ttttttatgg aaattaaatg
     1021 gttaaatcct gatagtcctt ttgatcgact tattttagct ggtgatattg gtggtacaaa
     1081 taccaaccta ggtcttgttg gttataaaaa agggaagttt actcttattt tagaaaccgt
     1141 tttcccttct gctacattaa ccagtttagc agaaccttta gctgagacat taaaaattgc
     1201 gaaagagcat tctcctgaat taaaaccaga atactgctgt attagtgctg caggtccagt
     1261 ttctaataat cgttgtgtga tgactaactt atcttgggac atcgacggca atgcattgac
     1321 tacgattata ggcattcctg tgatgattat taacgacttt gtagcgatta gttatggaat
     1381 ccctacttta gatgtggaag accccactca aattcataag ctagcgcatt cagatggtag
     1441 ttatgcagag cctcagcctg caacaaaagc ggtgattggt cctggtacag gcatgggtgt
     1501 ttctttcttg gcttttgatg ggaagcgtca tatccctgca agttcagaag gcggtcacat
     1561 gtctttctct ccttttgatg aagactcgcg ctctttttgt gattacatga ctaaaaagtt
     1621 aggaacgcag ccaggagtag aagtttttgt ttctggtctt ggcatatcta atatgtacga
     1681 atggtggaaa gcaacgaagg gaattcctag caatccatta tttcaaaaaa tagaagaaac
     1741 agaaccaagt gataggccaa agtatattag ccgtgcgagc gataccgatc ctgtggccgc
     1801 tgaaatgatg cgtttgtttg taaagatgct agggcgattt gcaagtgatg tgtcggcgct
     1861 tttgctccca ttaggaggcc tttatttagc tggtggtaca gtgcaaaaag atatgcgctg
     1921 gctcgaacga gaccacttgt ttatgaagta ctttgaaaaa aattacaatc caaatattcg
     1981 tccgttgcta cagaagatgc ctgtatatat cattaaagat tatagcatca gtctttatgg
     2041 ggcagcaaac gcaagtttga atttacagca aactaaataa tttttttaaa cttacttttt
     2101 ctctcatttt acgggctttt tagatgatta cgtctaataa gccctttttt atgcctgttt
     2161 tagtgaagct gggtttaagg caccctttat tatattttgc ttaaaactat tttttagaaa
     2221 aaataaggca aactctaaaa tatcgaacta gcgagaagta tcgctgcaaa tgttttgaaa
     2281 atcaatttct agagattgct gtataattgt tgagctatta aatattaaag gaatataaat
     2341 atgccttctt ttgacccaag tgcaaaaaag atgttgcttt ctggaaacga agccatctct
     2401 ctttcgatgt tgcattgtaa tgtgcaaatc gctgcggggt atcctggaac cccttcttca
     2461 gaaattctag aaacgtattc agaaataggc ggttatgctc aatgggctcc aaatgaaaaa
     2521 gtcgctgcag aagtggcttt gggagcttct tttggtcatg caagatctgt tgtaacgatg
     2581 aagcacgtgg gcttaaatgt ggcaagtgat gtcctttata ccgctaccta ttctggtact
     2641 cagggcggca tggtatggat tgtcgccgat gatcctggtc aagcaagttc tcaaaacgag
     2701 caagacactc gtaatcatgc gaaggctgct gtgtgcccta tgtttgaacc ctctaattct
     2761 caagaagcct atgacatggt gcgtctagct ttccaaacga gtgaacgctt tggcatccct
     2821 gtgattctta gaatgacaac acgagtggat cactctaaat ccatagtcca agtttctgat
     2881 gaaaagctag aagcattaaa acctcatttt gttcgtgata ttgcctctcg agtgatggtc
     2941 cctggttatt ccaaaccagc agcaaaaaga cttcgtgcta agatggaaga aatggcggct
     3001 tggaatagtg cagaaggccc aaatcgaatg gaaatgcgtt ctaccgattt agggattatt
     3061 acttctggta tttcttacca tcatgttaga gaagcggctc cagaagcgag tgttttgaaa
     3121 atcgggatga gttacccttt acctgtgaat ttaattcgtg aatttgccgc caaagtgggt
     3181 cgtcttgttg tgatagaaga aaatgaccct tggttatttg agggaatcag tgcactaggc
     3241 ataaaagtag aaggcaaata cgatccgatt tatcgttttg gagaattaga tgtcaatcgc
     3301 gtgagacgca ttttagctcg cgacaattcg cctgaaccag tgcctgtcaa agggcgcgcg
     3361 cctgcgttat gtcaaggatg ccctcatcgt gaatcctttg cggttttaaa tgaactcaat
     3421 tgcattgtct ctggagatat tggctgttat actttggctg ccctacctcc tctttctgca
     3481 atggatatga tgattgatat gggggcttca attggtatgg gtctcggctt acgccatgta
     3541 cttccaagag aagaagccaa gcgagttgtc tctgtgattg gggattccac tttcatacat
     3601 agtggcatta cgggtcttgt agaaatgggt tacaatagac catctactgg ccacgtcgtt
     3661 attattttgg ataatagcat tacggcgatg acagggcaac aagagcatcc tgggacaggc
     3721 cgcaagttag atcattcgcc agcatacaaa gtcaattatg gtgaaatcgc aaaagcagca
     3781 gggattgaaa atgtatttga agtcaatcca gtgaaagagt atgataaaat gaaggcgcta
     3841 gttaaagaag cgttagaaaa agacgagctc actctcatta ttgcaaagag cccttgtatc
     3901 ttagcattaa agagcatttt agcttgggat aaagcaaatc gtgaaaaagc aggagatcaa
     3961 aaatgagtac catgaatgta aagtttgctg gtcttggtgg aacaggtgtt gttaaagcaa
     4021 gtgatgtttt tgctgagcta gtttttgaat tagggtatga tgtaaaaaaa gcagaagttc
     4081 atggcatgag ccaacgtggt ggttctgtgg caagtgacgt tcgctttggt gaaaaagtag
     4141 aaagcccaat gattcctgat ggagaagcac aatttctcgt tgttttcgct gaagatcaaa
     4201 ttccagtgaa tgaagcagca ttaaaagttg gcggcactct tttatctcca aaagatatag
     4261 atgtttctaa actaccaact gaaaaagcgc tgaatgtcgc tatgcttggt aaactgagta
     4321 cattttttga tttccctata gaaaaatggg aaaaaattct tcaaaagctt tttgccccta
     4381 aatttcatga aggcaattcg attgcattta aaataggacg agaagggtag aatttcttac
     4441 tttggctgta aaaaaactac agtagtgtgt aagaaaaagt tgatttttaa gccttttctc
     4501 gatgagattc aaaagcattt attttcaata agcctctaaa atccttaaaa cacaaggttt
     4561 ttagaggttt tttattttct agagataaaa gaacagctta aattcgtgta aaaataattc
     4621 atatttcaat agattcattt attttatatg aggcacattt taaaaatgtc tttattacta
     4681 ataatgcttt ttagaagtgg ctatgggtca aaaaaaggat gttttagatt ggggtttata
     4741 tgatcaatac aaaagtcggg tttaagatta cacttctctt gttattaatg acaagctttc
     4801 tatttgcagc aaaagaaatt tatctcccga atttttttaa gcaacagggg attgattttg
     4861 agtcgccaaa tgcgcagtgg agccgtagtc gtatggatag tacggccaat tggattatcc
     4921 tttgggagtc tggttttggc agtgatccct ctagtaatgc agctccatat aaagtgaata
     4981 tgagtcaatt aaaagaggtg cttgagaaaa gctatgaaat gaatttaaat acaatgaaaa
     5041 tggtcgtgaa gggatcttcc aaaacagacc agtataaaat gattgttttc ataaaatatt
     5101 ctacgacatg gggagcctat ggatctggtt acgataatgt cataggttca ttagaagtaa
     5161 atccagaggc tgctaatatc cctagtgtag tcgctcatga aatagggcat acttttcaat
     5221 accaagttgg ctgtgatgtt ccagaaggtg gctttcgtta tgggttaggc gctaatggcg
     5281 aaggcggaaa cggattttgg gaacaagggg cacaatgggc atcatttcaa gtgtacccag
     5341 aagaacaatt tttgacatat gattttaata cctatataaa aagcaatcat cttcatattt
     5401 tacatgaaga acctcgttat gccaattact tcttacctga ttattgggca tataaaagag
     5461 gaattgattt catgggacgc ttatggcgag aatctcgtcg tccagaagat cctgtagatg
     5521 cttataaaag actttttaat gtttctcaaa gtgaatttaa tgaagaaatt tttgaacacg
     5581 ctgttcgttt aactacatgg gatcttcctg cgattaaaga aaggggggcg agttatattc
     5641 aacgtcgagc tcaggtgaaa atgaaaaaaa caacagatca atattggctt gttgattctg
     5701 ctgtcaccat agaaaattat ggctataata gtattaagtt aaatgcccca tcaactgaaa
     5761 ctcaagtctc tgtatacttt caaggaaagg caggagccag tgggtatcgt tctataaaca
     5821 tcgataaagc gggctggtat tttggttttg tagctctttt aaataatgga gaaagagttt
     5881 atagtgatgt agggtctctt gagtatagtg gagggaaaaa tccagaaggc gcattaagtt
     5941 ttaatgttcc agcaggttgt tcttatttgt ggctagtcgt ctctggagcg cctcaagaac
     6001 attggcatca cgtttgggat gattccactt ataatgatga acagtggcct tatcaagtca
     6061 agttttccaa tacgaattta cttggagaac aaaatgaatc tacgacgaat tattctttaa
     6121 gtaccgctgt agatggttct ggatcgattt ccccttcttc gggaagttat ccatcgggat
     6181 ctctcacctt aaaagcaacg ccaacgcctg ggtatgtttt tgatcattgg agtggtgatt
     6241 tgactggttc tgaaaaccct gcagtactaa aactagatca agataaaagc gtcaccgctc
     6301 attttatcaa gttaaagaca acagtagagg aatttacttt tcagctcacg atgtctccta
     6361 aatcagatta ctctcctact gcagttcctt tagataaaag cgctatcgct ttagcatttg
     6421 gtttatctga atccgattta gtcgaaaaaa taggaacaga tattcgttat tatgcagtga
     6481 atccagatga atcttttgat gcaaactcaa cggcaataga tcctgggcat tggtataatg
     6541 cagaaggtca agtggtgagt tatggagatc aagcggtaat ctattctgag attaataaaa
     6601 atacactaac agccaatatc ggacagtatc ctgatcgttc tgttaaaggg gattcttacc
     6661 ttataaaaca agcgcttgtg tatcaaaaat cagctacaga agaagtgcaa gtccatttga
     6721 tttattctat taaaatagat gagtctctaa aaatcagaac acgagcattg cctccaattc
     6781 taagccaagg ggaacgacct attttatcat cagagattct ctccttaatg agtgctggcc
     6841 gtggagaact tcaagtctat aatctaaaag ggaaactcat ggcctctgta aaacaggttt
     6901 cttcttggag tgatcttgga atgaatcaga atggtgtata taagattgta tttttagaaa
     6961 ataataaaat caaagcgatt caatccattt cctatgtcag gtagagtccg tctaaagcat
     7021 ggaatgaata ataaaccacc atttttataa aattgaattc gaaattcagg ttatacgcct
     7081 gaatttctta tttttagggc tcatttaata aaatcagggg ctctaaattt ataattttca
     7141 atatatggca tgttttttgc atcggacctt aaataaaacc ttaaaaaatc tccaatggat
     7201 tcttattgga ggaatttctt ttctgtgtgc gggattattt tctggctgtg aaaaaattga
     7261 agaggatgcc ccatggataa atgtagagcg tcctttgatg ctttttaacg atactactct
     7321 gcttgatttt tatgaagaag aagttttgtc gtggaaatta aaaacagcgt acctagaacg
     7381 ttgggcctct aatgataaag tttttgcacg tccaatactt gttgatattt ttgattcgct
     7441 tggagaaaag gtagcgtttc tacgtgctga ttctggtagg ctcgatatgg ggatgaatta
     7501 cgtttttgcg tatggacatg tttttgcttt tactccaaag ggagcttctg ttcgagcgga
     7561 ttctcttttg tggcacaaaa gagataacat ggtaaaaaca gatagctatg ttcgagtggt
     7621 tacagaagat ggagacgttt tacaaggaaa aggttttatc agtgatgcta aaatggataa
     7681 ttggagaata ttatctgagg tgacaggtat ttttcaagat gccgctaaac gtttaaaaga
     7741 agaagataaa acgttattag aggagacttc ttcaccatcg agtacaagag aaaagccttc
     7801 tactaaaaat gaaaaacaag aatcggtctc taataatcca cgaatgaata aagcaaaaaa
     7861 aacagatcgt gaagttcgtg ttttaactga taaagaggta gcggaagata ggcaaaaacg
     7921 tagtatgctt aaacgtgatt cattgagaaa ggagagagga atagatccaa aatcgaaacg
     7981 ccctcgagca aggagaatgg ctgaatgagt cgctatcatt ttagcattct atttctatta
     8041 ctcttttgta gtatgttatg tgctcaaagt tctcctttga ttatgcgtca tgcagatagt
     8101 ttatctgtag caagaactag aggtagtttg ttgttacaag gaaaagtcca ttttattcac
     8161 gatagtattc agtttaaaac gcagagagct atttggaatc gcgatattga tatggtccaa
     8221 tgtgatgggg gctttttatt tactcacaca gatggtttca ttagagctag cagtggcgtc
     8281 tatcagaaga aaaataattt tgcttcagcc acagggaatg tagttgctgc tgactctcaa
     8341 aacacgtatg ctttttttgg agagcattta acatatgatc gaaaagaagg gatcttaacg
     8401 atgcccgata aacccatatt gcatcaatat gaaatgaaag gcgatagtat agacacctta
     8461 tcgattaagg caaagaagat tgtatatcat aaaaagaatg aatttgcttc cgcttttaga
     8521 gaagtaaaaa ttcaacaaga aaaaatgctt gtgacttgcg ataccgccta ttttgacaaa
     8581 aaaaataatt ggatgtcttt atcgggaaat ccgatttgtc atttagaaaa tgaaatgatt
     8641 caaggtgatt ccatttattt agttttagaa cccgatggaa aaagccttaa atcggcatta
     8701 gtgattcgaa atgctcatgg taagcaagta caaaaaggaa agggacgtca accagcgcaa
     8761 cacacagagg cttttggaga tacattgttt gtagaatttg aaaaaggtaa aataaaacat
     8821 ctttatgtga atgtgaacgc caagggattc ttttttgaag aagatttacc agattataaa
     8881 aatctaatgg atggtaatcg tttagatata tattttgaga agggaaaggt aaataaagcc
     8941 attgtgggcg gaaatgctca aagcacatat ttttatgtga aagaaaatcg cagtgtatct
     9001 ggtcgaaatg aatcttctgg agatacgatt caggtggatt tcaaagatgg taaagtaggc
     9061 tctcttagaa tgattggcgg taagactctt gctagtgggc gctattttga tttagagaaa
     9121 agcgtgggta aagaggataa aagaaaagag tttaaaagag attctactat agttcgaaaa
     9181 ccggagtctg agagaagaag aaatagaatg gaaagaataa aaaaaaatca agtgattctt
     9241 tcgaaagata gtctaagagc gagaaaaaat ctcgaaggga aaaagagcga tgaatgatgt
     9301 gatccataca atacatacaa ataaattatg caaagtttat agcggaaaac aagtggttca
     9361 tgaggcttct attcgagttt ctcaaggcga aatagtcggc ctacttggcc ccaatggagc
     9421 agggaaaaca accacttttt atatgattgt gggtatgatc cgtccagata gtgggcaaat
     9481 atttttagat gatattgaaa tgacttctaa accaatgtat cgtcgtgctc gcttaggagt
     9541 ggggtatttg cctcaagaag cttctatttt cagaaagatg aatgtcgaag aaaatattat
     9601 ggctattctt gagacgcaga acctaaaacg agtagaaaga aaaaatcgtt tagaagaatt
     9661 actaagcgaa tttaagattg atcatatacg aaaaacaaaa gcctatagct gctcaggcgg
     9721 agaacgtcgt cgtttggaga ttgcacgagc cttagcatcc aatcccaaat ttttactgtt
     9781 agatgaacca tttgcaggta tagatcctat tgccgttgca gacattcaat caattatttc
     9841 tgatttaaga gataaaggca tgggaatttt aattacagat cataacgttc gtgaaacgct
     9901 ttcaattacg gatcgtgcct acattatgta taaaagccag gtgctaacag aagggtcttc
     9961 tgaattttta gctggagacg aagaggctcg tcgtatttat cttggagagt ctttccgttt
    10021 aaattaggga gaagatatga atctgggaat gcaattaaat caaggagttc gtttagagca
    10081 gacgttgtca ccacagatgc tccagtcgat cacgattttg cagatgaata cgcttgatct
    10141 tgaaacggcg attaaacaag aaacagaaca aaatccactt ttagaattag ccgatacaga
    10201 agagaaggat gaagcggact ctttagtcga agattatcct gaagactatt ccgatgtgaa
    10261 ttcagatagc gatgatgttg aaacagggct tattgatgat ccagatgaaa aactaattga
    10321 ctgggacaag tattttgaag aaggttttgt agatgctgat cgtcccttaa aagacttgaa
    10381 tgttccagat cctgaagaag aagagtggat ccgtcaaccc acagagtctc agagtatgca
    10441 agacttctta aaaaatcaat taagaagttg gaagcgtcca gaacgaattg ttgttgtggt
    10501 ggaatatcta attgattgtt tagatgaaaa aggatatgta caaagcgcat tagctcaaga
    10561 tgtttccaca ttgcaagtgc gcagcggttt taaagaagtt gaagaaattt caccagaata
    10621 tttagcgagt tcaaatccag atattttaga agcagaagaa gttttacaga ataagaaaga
    10681 actagaagag gcctcctttt ttgtacgtga agcatttcat attttacagt ccttaagccc
    10741 cagaggaatt ggagctcgaa atttaaggga atgtttatta attcaggctt acagtttgcc
    10801 agatttttct cttttagcga ttcggatctt agaaaaacat tttgaatctt tgaagacttt
    10861 acgctatggt gttattgcaa aagagcttga tgtcacgaca gaagaagttc aaggagcagt
    10921 tcaggaatta tcaaagttgt ctcctcatcc aggcgtacaa atttcgaact cttctattca
    10981 aatgattgtt ccagatatga gtgtggtgga agaaaaacca ggcgttttta aagtcattgt
    11041 aaaagaagct ccttttcgca gtcgtttacg aatcaatcaa acgtatcgta agcttttaat
    11101 gaattctagt acttcgaaaa aagataaaga atatatacga gaaaaattaa atgctgcaaa
    11161 ttccttttta cgtagcgtgg ataatcgcca gagcactatt gagcttgtaa tgaatgctat
    11221 tcttaaacgc cagatggact tttttattaa ggggccagaa tacttgcatc ctatgattct
    11281 acagaccatt gcagatgata tacagcgaga tccaagtacc gttaatcgag tcaccaacgg
    11341 caagtatgtt gaaacgccat acggcatcta tgagttaaaa cagtttttta caaatggctt
    11401 aaaacaagac gatggttcag atttaagttc tgctagaatc ttaaacgcca ttaaagaatt
    11461 aatagaaaat gaagatcgta aaaaaccact ttccgatcag gctttaacaa atgcattgga
    11521 agagaagggt attcgtatcg caaggcgtac ggttgcaaaa taccgagaag aagagctaaa
    11581 aatacttccc gcaagacttc gaaaagaggt ttaggggtag ctttaaatcg aaaagacaag
    11641 gaaaaaatgc gttttctata gtccaatttg gaactgggtc acagaaagtg tttttaaaag
    11701 agtttatttt tatattaata gaacgctttt cagagaaaaa caaatttttt catgcttttt
    11761 tgtgaaaaat agatgtatat actctgaatg caaaacattg gttttgtttg aacaacgttt
    11821 tttaacacgg aggatccatc atggatatta gtatttcagc tcgtcatttt aacgcttctg
    11881 caagcttgca agctcgtgtt caagaagaga ttgaaaagtt gggcaagttc tacccaaaca
    11941 tcacctccgc cactattgtc ttggatcatg aagtagagca tatgcgccgt tgtgaaattt
    12001 ccttaaatat taccggtaat gtggttgtcg cttctgcaga cgaagaaaat atgggcaagg
    12061 ctctagatgt tgctcttgaa cgtgctaaga cacaattaaa gaagcaaaac gaaaaacaaa
    12121 aagatcataa aacacaatca ctgtctgatt tggtgaataa gtgagtgctt attcgttaaa
    12181 agatattcat gtgaattgtc gggaaaaata tcccgttaaa aacttatggc ttaaacacaa
    12241 ggattttctc cggttggaac tctgtactgg agaagattcc ttaaatggtt ttattgaaga
    12301 aagtggttta catcgaccag ggcttgctat ggcgggcttc acatcggtgt atagctcaca
    12361 tcaaattcaa gtggtggggc ataccgaatg gaattattta gaatcgcttg gctctgaaaa
    12421 acgccgagaa atattttcaa atttatctca attccattct cctctttggg tgattactca
    12481 tgagcaaaaa cctcatcaag aattgcttga tatgtgtaac gctcaaggga cacccatttt
    12541 ttgtaccccc ttgtacacgt ttgaatttgc aaatattgtt caacgtattt tagaagactt
    12601 ttttgctcct tatgctgtta ttcacgcaag tctagtagat gtttatggcg tgggcatgct
    12661 ttacattggt gaaagtaatg tgggcaagtc agaatgtgtt cttgatttag tagaacgagg
    12721 gcatcgttta gttgcagatg atgtcgttca tatctctcat attgggaatt ctattatcgg
    12781 tcgagcgaat cctatggtaa aacatcacat ggaaattcgt ggagtgggga ttatcgatat
    12841 tcgttctatg tttggaattc atgcggtacg taaaaccaaa aaaattgaag ttattgtgga
    12901 attgcaacag tgggatcgcc atactacata tgacagaaca gggcttagtt cttccttaga
    12961 agaaattatg ggtgtaaaaa tccctcatat tttaattcct gtgttaccag gaaaaaattt
    13021 aaccgtgatt tctgaagtga ttgccatgaa ttcattaatg aaaatggatg gtatggatac
    13081 cgcctcttct ttcaatgaac ttcttttacg tcgaattaaa gaaaaagcta aaggcaacaa
    13141 agacgattac atggatgata gcatctttaa tggcaatgca tatgaatagg tttgtttatc
    13201 tatttaagaa taatttttta gctgttcttg tttttctttt gttagcggcg attgcttttt
    13261 ttgcctggtt tttctttcac ccatcaagtc cttatcacga acgccaaaca tttgtgattt
    13321 attttgagaa aataggttca cttgcttcag gtagccttgt tcgcgtgaat ggaatggaaa
    13381 agggtaaagt tctaagtaca gaattaacag aagatggggt ctatgtaaag gtgtgcttat
    13441 ttgccgatta taacgttcct aaaaactctt catttcgatt aatcaatact ggattagtgg
    13501 gtaaacaaga ggtctctatt cttgcaggag atgcttcagg ctttttgatc tctggagata
    13561 cggtgaatgg tcttacagac cagggttttt cggagataag ccgaaattta tttttgatat
    13621 taaatgaact cgatacaata aaagtaacga ttagttcctt tattgatacg attacaaatg
    13681 gttccatagg gaaatctctc tattctgttg aaaagaaaac tcgtttttta actcaagaca
    13741 ccaaaaacaa agtaacgtct tggattaccg aattaagaaa agtaaaagag gctgctctct
    13801 ccataattcc agaagatccc aaaataaaaa aggatataga cctcttatta ggtgatttaa
    13861 atcaattaga aaaagaatta gagcaattac aacaagaatt agcctccatt tcctttgaaa
    13921 taccagaaaa aggcactgta gggcaaattc ttaaagactc aaacccgttt ttaaaaaaga
    13981 ttgactcact taaagtcaat attgatctgt tgatagaaga gatcaaaaaa aagggattgg
    14041 ctttgaatat cgatatattt tagtcttttc aagaaaaatc aaaaatactc cttcttcttt
    14101 gtaaaaaaaa agataattta tgcgggtacc gtcaacaacg atttcaattt atggagtcgc
    14161 aaacgaaggg gttcgtatga cagaaaaaat accggtaaaa atggatcaaa aagatttgga
    14221 tttttttgaa gctttacttg cagagcgtcg cagagagctt gtatccgctc aaatggattc
    14281 agaaaacaca aatgtatttc attcgcaaaa agaccaagga ggagaactcg caggttattc
    14341 aaatcatttg gcagatgccg cttctgatta cacctctcta gaaaccaatt ttgatttggc
    14401 tgcacgagaa ggcaaatatc tagtatacct agaagaagcc atacagcgtg taaaaaatgg
    14461 tacttttggt gtttgcaaag tttgtaagaa tctaatccct aaagcacgct tagaagctgt
    14521 tcctacggca acaaagtgtg tggattgtaa agaagaaact aagcgtaaag agcgtgaaga
    14581 taatcgtatt gaaatggctc gtctttttgc tgagcaacaa aggcgagaat taaaaacaaa
    14641 aaccaataat taattcaagc tttcaaaaaa taaatcgctt aaataagacc ggaaagtata
    14701 aatcctttcc ggtttttctt ctctatgggg ccaagtaaag ttggataaga gaattatagc
    14761 cgcattttgc tcaggatctg caacaatact tgctcctgtg aaaccagttt ttccaaaagt
    14821 tctagaagaa caacgagttc ccataaaacg agggttattt aattcaaatc caagagcggt
    14881 ttgagcgttc atagaagggt caagggcatt cctagagatt atattcaaaa tgccagcagg
    14941 catcacctgt tcgcctttat ataagccatc ctgaagaagc atttgaacaa atttcattaa
    15001 gtcttcgatg gtagaaaaca tacctgcaga acccacagga aacaactgtt ctaaaataaa
    15061 agcactttca tcgtgaacta cgccttgtat gatctctttt ctaaaggcac agtgttctgt
    15121 aggaattatt tcagaacgag acattctttg caaaggaaac cagccagaac gagtcatatg
    15181 aagggggtcg aagagaagtt ctttacctag aaccgaaagc gatttgccac taaagcgttg
    15241 tagaagaata cctaataaaa tactcgcagg gttcccgtag ttaaaaattg ttccaggagc
    15301 cttttcaaaa gaataattgt ataagtgatt aagaatttct tctggagaca agtgttttaa
    15361 agaactcatc ggaatacgat aatcaaggct atgcgtgagt aggtgtttta aaagaatttg
    15421 atctcggtaa tttgtttgta actcttgaat ccaagaagct acgggggttt caggagataa
    15481 ttctccttta agaatatagt gaagagcaat agtagatgtt gggcagacct tagtgagcga
    15541 agcgatatca aaaacagaat cggggcaggc atttaaagaa atgaataacg tttcttgatt
    15601 tggcagcaaa acgccaacga ctgctttatc aaaaatttga gttgattggg cttgacttaa
    15661 agcgagagag attttttctt ttaagctatt catggtttta ttaaaatagt taagttcaat
    15721 gatagatgct aatcttgtgg tgaacgtaag tatgcttgta gcactctttt tgattaataa
    15781 taatgctttc tcttgcacaa ttatttctat tatgtttttc tcgcttgttt tttcgaaact
    15841 cggtaaatag gatctatcat cattttttaa gaagtctttt actagaaaga cttctatatt
    15901 ttttattggc gtatggaaga atatattgac atattaaatg cgcatggcga accattaaac
    15961 tatagtgctt ccagaacaga agtacatgcg aaaggtttat ggcatcgtac ggttcatatt
    16021 tgggtttttg acctgaatgg aaaaatcttg tttcaaaaac gaagtctaaa aaaagaaaat
    16081 catccaggat tattagacac gagttgcgca ggtcatattt ctgcaggaga tgatagccga
    16141 aatgcgggcg tacgagagct tgctgaagaa ttaggcatta tcaagaaaac ggatgatcta
    16201 aaatttcttt ttgaatcaaa acatgaaacg gttttaaata atggttctta cttagataac
    16261 gaatattatg atgtttatcg ggtggatctt tccttaaaag aagtagaaat gcttagccct
    16321 caaaaaagcg agttagaatc gctacattgg ttttcaatta aagagtttca agatctttta
    16381 aaaaaagaac cttccctttt tgtagaacat ccagaagact ttaaatactt aaaacgcctc
    16441 ggttagaggc gtttcgttat aatcaaaaaa tgggtttcag cgaataagta gaaagatttt
    16501 ttttatcgtt ctttacttta tagcaggaaa aaacaaaaaa agaactgctt tattttatag
    16561 ctgggaaaaa taaaaaaaga acagctttac tttatagcag gaataaataa aaagagcttt
    16621 tgaccttttt ttatagagac ttgcttaaag gtgaacctag aaactgtagc tcctctagag
    16681 tctaaagcag caatgtcaag atcatgttgc cctggaccta caggaatgcg agtcatgtga
    16741 atacgattag gcataaaaag gcctacacgt aagtctgctt tttctaattg actagaggcg
    16801 acatctgttc caatacttgt ggctaagttc aaaagaggat tatttgtttc aaccgctttt
    16861 tttgtctttt gagcggcaaa agtacgtaaa gcgacacgag ctgctgtctt tgcaaccgta
    16921 cctgaccaat cgtcttttaa attgcgagtg atttcatcgt ttaagtctgt cacattttct
    16981 gaaaaaattt ctggctcatc atttaagtag atggagaaat cataaacatc aaaaggaagg
    17041 acttgttttt ctggtaaact aaaagcgatg tgagctgttg ctgtacctga ttctgcaatg
    17101 ataggcgcaa tcatagtgaa actcttaatt tcacctgttt gagcgtctgt ataggtgaga
    17161 ttcaaacctc cgccattcac aaaagttcct gacattctca tttcaccaag aataggacta
    17221 tgcccagcgt aaccgatgac aatgatttct gcacccttct cttcaaggct ttgagagaga
    17281 ggggtttcag gaatattatt catcttaaga agttttagat cttcttctct acccgttttt
    17341 tgaagagtgt aggacataaa atcaacaagc tcaacaggta atttttgccc gtactcctta
    17401 tacgcttttg ctgccttata ataagaaatg gcggcgttat caatctctcc gttcatctca
    17461 taaacaagag cagttaagta ttgtaaaaag ccattgtcat tgaccttttc actatctttt
    17521 tggtaaaggg cttccatagc catttgtgct cttttgactt cgacgttggc gccgtctaga
    17581 tcgttgatag ctaggtaatt caatatttga acttgataca aaaagagtaa ttcaaaaggc
    17641 ctagcgcgat aaggacgagt attatcattt gttaaaagag cagcggcttc atttgtgact
    17701 gatttagcat ataaatcatc ataaacttgt tccgctttgc tcaaatgctc aatgcttttt
    17761 ttaaagtctc tattgtagtg aaaaagcaca cctaaatcaa aatggtacaa aaaggcgctt
    17821 ttctcaccat agagatcttc tttctctttt tcaatggttt gaatggctcc agtaaagcca
    17881 ccctgtttta aagattgaga taaagcatca tatcgaacgt ttgatttgtt tgcacaacca
    17941 aacaatagaa tgctgcaaaa agcaatgaga attatttttt tcatgacaat aaaaagaggg
    18001 aaagattaaa gttttacagc atctctagag atatatttct tgatcttttt gttgccaatc
    18061 caaactttgc gatggctttg aagatcagta agttctaagt caatttggta gaaaatcact
    18121 tgttcaccac catcttggtc gataatagaa tttagagaac cagtgagcat taaatcagcc
    18181 ccaatttctt ggccagcatc ttttcttgtt tcgtcggttg cgtttccagc ttggctttcg
    18241 atttcattac gaagttcttg acgttcagga gcactggcga caaaatcaac cgtaccagag
    18301 ttaattaaag cacgttccat atctttaata aaagtctcta tattaatgtg ctcgtggctt
    18361 ttattgcgaa ctttaccaat aactacggtt ggatttgccc ctttttgttg gatcatatta
    18421 gaataccatg ggcggctaag acagtcttta atcatttcat ctgctactaa gcgagaatca
    18481 gtgtcgttcc atcgacccga taaatcgaca acagcattag gatcaatgcg agagactttt
    18541 tggttactgc tgcaagccac taaaaacaat aggctgaaaa agaatagaac gcggaaaaaa
    18601 ttgttcataa taaaactcct tttaatctaa aatgaaatta acatttttta catcatttta
    18661 caatgatgat aaaaaaaaat ggactgtttc tttacctatt ttattgaaat gtgctatttc
    18721 gcacttgatt gctctttact gtaatggcgt aatcgaagga gtgtggcggc aatttcttga
    18781 gttctaggca aagaaagatc acgactaaag ccttgaggca atttcctaaa cccgacattt
    18841 tgaatttgag cacaccattt ttgaattaat tcgaaagaag gctcttcagt tgcattttgc
    18901 aataaattca aaagaatata gcctgcacca aaacgttgag atgtattaca aaagcctgca
    18961 attttattgg tttcgctaag taaaaagccg atctgaaggc aagtgtcccc tttcatttta
    19021 atcttcactt gacgttccac aggtgaggat ggtcgaacat ggggtaacaa aggctgaatg
    19081 tatgttaaaa aagaacgact ttcagaaaaa tggaggggaa gaacaacgtt ttcttcttcg
    19141 gctagttttg ataaaggagg attttgctca ttaattaatt tcgctttggc tgttgcgttt
    19201 tctggaaggt agttattcac aagaattact tgatctgcga cttgcaaata atctccacag
    19261 gcgccagcga ctaataagaa atttaggcca tgttttttaa gagtaacaat tcgatcaatt
    19321 aatggaatga gaggttctct ttcagatcca ataaga
//
LOCUS       DAOOBB010000012        18905 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_3871_length_18905_cov_4.117613, whole genome shotgun sequence.
ACCESSION   DAOOBB010000012 DAOOBB010000000
VERSION     DAOOBB010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 18905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18905
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3871_length_18905_cov_4.117613"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1682)
                     /locus_tag="PLT31_01245"
     CDS             complement(<1..1682)
                     /locus_tag="PLT31_01245"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPW93788.1"
                     /translation="MMETRYNPQEVEARWHSEWNKKKSFTPSGKGTPFSVVIPPPNVT
                     GALHLGHALNNTIQDILVRYRRKTGCDTLWIPGTDHAGIATQAVVEKRLLQDEKKTRR
                     DIGREALVERIWKWKEEYEARITKQLKSLGSSCDWDRQRFTLDPICAKAVRHAFFNLF
                     QKGLIYRGKRLVNWDTHLQTAVADDEIYYETVKGFFWTFKYPLADGSGFIPVSTTRPE
                     TILGDTALAVHPDDERYQKYIGKMLKVPFTDREIPVIADGLLVDPEFGTGSVKVTPAH
                     DPNDYATGLRHKLPMINIMNDDGSLNENAGVFCGLKGKKAREAVVQALEEQNLLIKVE
                     DHEMQVGHSDRSKTAIEPYLSDQWFVKMDSLAEKAMSAVQNKEIEIIPERYAKNYLDW
                     LGEKRDWCISRQLWWGHQIPIWYCATASEEDLKKAFANRKDVFYSKKEQGGWLICSQE
                     SDLKENEIEGHRVERDPDVLDTWFSSALWPHSTMGWPESTPELKKYYPTSVLVTSRDI
                     ITLWVARMVIFSQENMGTIPFKKVYIHPKIMDGQGRTMSKSLGNVIKPDDVC"
     gene            2021..2371
                     /locus_tag="PLT31_01250"
     CDS             2021..2371
                     /locus_tag="PLT31_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93789.1"
                     /translation="MDSIQMTCPHCGLSMDVLLRGKPSSMIVFICSRCKSPLMRYEGE
                     IFELDREEFNSLRKKLSRVLDALVEESSDIKREGLAEIPLWNSDDSITSEAVDEMLKL
                     LEESKDVDDFINQI"
     gene            2374..2979
                     /locus_tag="PLT31_01255"
     CDS             2374..2979
                     /locus_tag="PLT31_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HPW93790.1"
                     /translation="MWSFFLASLVVALVPGPDNIFVLSQSASFGRKAGLVVILGLCTG
                     ICIQILFLVTGLSLILVNSPKAFFVLQCLGACYLLYLAFLSFRVQKGVISVEKTKALS
                     PFKLYRRGAFMNLTNPKAVIFLLAFLPPAVNTSLSLSPTTQIIVLGLLFILSVWIVFG
                     AFAFFGGWIYHFFSSSPKANLYLNRAAGAVFIFLALSLFIW"
     gene            2989..6771
                     /gene="hrpA"
                     /locus_tag="PLT31_01260"
     CDS             2989..6771
                     /gene="hrpA"
                     /locus_tag="PLT31_01260"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HPW93791.1"
                     /translation="MFDSSLLKIEYPDLPVVEKKEEFLSLLKNHSVVIVQADTGSGKS
                     TQLPKFILESGIAAQGRIGVTEPRRLAALSIADRLREELKDESLVAARIRFLEEGSAK
                     APIKVMTDGILLQEFRRDPLFKQYSVVMIDEAHERSLNIDILLGIFKNVLEKRPDFRL
                     IIASATLDGELFAEFYQNAKILKAEGRLYPVDVEYRFLTPEEEREESDINLTEQARDA
                     ILELLSRARDHLLCFLPTERDIQDLAIELQKDLDSNTFEILPLFGRMSPKEQRKVFQS
                     TSKTRVVLSTNIAETSLTIPGIAYVVDSGLARVSRYNAQARIQGLPIEAVSQASARQR
                     TGRAGRVKPGTCIRLYDEKSFSERDLFTEPEIRRSNLANVVLQLQSLGLEVENFPFIQ
                     PPPRSAFRGAYKTLHELGALSIAEAGASVTDFGLEMAKLPMDVALSAVLLKAREFSVL
                     QPAVIISAALSIQDPRVLPSEEPERTRAREAHRRFGGHKSDFLLYLSIWNAFSEDFEN
                     TSWNKLRKFCEKHYLHFLRVREWIDLYEQYCRLLKVQYKDRVCSLNSFHRDFLHMALL
                     AGFLGGIARRDIENSCYRLTNGRETHVFPGSDLYGKSVEWLFSAEVRETSRVFLTKNA
                     EIRSDWILKVAEPFCTRRYYEPEWNRERGFVEIREEVSFRGFVIAGGRRVDYARIDPK
                     ECARIFFREAVVLMNMARPFAFMEHNDRVIQNLHAQEARERKFGLAPNEEMQVDYLMS
                     VAPDVCSIRTLKTFILEHTDAALRFDPNYFIEQSHRGVEYHDSFQNKIQWPSKNQKQE
                     IPTLTEKGTSIEFFRIAGESVQGELVFNASQNNDGLSLKIPHTLIPSLSPAFLALQLS
                     RWRHWMLEAMIRELPKVNRKSVKNQFEEIDDHFCVKLEQHPNASPFLALYEALSEMKG
                     LDDIHLNLNPEKEHHLRLHLSFFREGYLEEKKVELSPEWGSFRFFKEIRSLISSFGVD
                     VNLGDFRYGYRLHENALMMPEESDFWQQRLRKIEKGACQQSVMLIMNRLAFIENAGLY
                     SNENPLPHKDEVFKTLVTEPFEPDELDRFSHLHFSRSKKVNDFKALQEQTKPEQEIIR
                     LILNRMTFESGLLGKEAFVRCYETLKRFCENTRGNRPVDPVFKKKVLAFPEISSQYNR
                     LKYIEDWLESGNSFSLVEDPMDLIQWKSIRESFRPYFVARYIKEDEQIKMRHLLSALE
                     KEMKSSNDYFEIWLEVQLLLEEFEYFKFKRSILFESDALEVEKSSIVKLKGRFGKL"
     gene            6811..7632
                     /locus_tag="PLT31_01265"
     CDS             6811..7632
                     /locus_tag="PLT31_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93792.1"
                     /translation="MKFFVLLLSFFALWGCDFSWFTKDPVVVTVGDVRLKESDLRAKV
                     TGWDSLSNREQIAWVEAWIDEELLYQEALNLGIHREKQIKERLILTERKIVVEAFISK
                     MTDSLSIDIKEAKRFYDTHPELFIYDKWLWSGFMLSYTDWKWASSYYKAKKESVFDSL
                     VHEDFRLKKIISFDSVLETPDSCLSTDLRELSLGLLTAPKVCNGFLKSIVITNRQDSG
                     AVIPFEQVKDQAHALAQINKQKETLAVLKTELKKKRPIFSNPQLFSDSLDQKRNK"
     gene            7629..8921
                     /locus_tag="PLT31_01270"
     CDS             7629..8921
                     /locus_tag="PLT31_01270"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF020872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPW93793.1"
                     /translation="MIHWRYLPLTLFLFVFAATPLAAKELLESIAAIVDGKPIMRSEV
                     LGGLYQLKNSPEAQSMNESEQMKYVLNHLIDEKVLLSRISRDSIVVSDEEVDQRVSMH
                     LNNLAASQNTDIATLEKAIRMQLGISVAQYRDQLAKQIRDHIYVARIRQIHVGAMTPT
                     KKEVVEFYNVYKDSLPRQYNCISVSHLQLNIEPGQSILDSVKKDALQLIDSLDRGMNW
                     EILAKRYSQDESAGKGGDIGFARKGTLDPEYERVMESLKNGQYADQPVKTPLGYHIVK
                     VLGRKEDGIRSAHILLKTIPSSQDSLKVIEQINTIREEIKTKEAFAEKAKQWSQDKET
                     NFKGGDLGWFQRSEFDTNYVQTISRLSVGDISEPVLIDNAYHLFRLDDESQVRSLNLE
                     EDYPKIEALAANQMETKKLESLVKKWRQEVHIEIRLKE"
     gene            8879..9679
                     /locus_tag="PLT31_01275"
     CDS             8879..9679
                     /locus_tag="PLT31_01275"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPW93794.1"
                     /translation="MAPRSSHRNSSQGIEVAAPYEKLAPYYKKIMEHVDYVDWAFYLN
                     TLLKKHKREPSSLLELGAGTGLLAEHFKPVSIKKRITTDYSFEMIRKASPKTAGYRVA
                     ADASSLPFTGVFDMMLMTYDAINYLSKSKIEDLFKEVFRLLSEKGVFIFDVTTEYNSL
                     TYFEDVVDVEEWANDFIIRRSWYESRSLKQYNQFDFFILQKDGSYLRETEKHEQTIHP
                     VSFFIKAAEKAKLEVEGVYADFTTETDLSYAERVHFVISKKKNTKARP"
     gene            9683..10372
                     /locus_tag="PLT31_01280"
     CDS             9683..10372
                     /locus_tag="PLT31_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW93795.1"
                     /translation="MIHFSHVTKTYEDNWKALSDVTCRIHKGEFVFLTGHSGAGKSTL
                     LKMVYMDERPDHDRGGQIMVKFTQDSVYDSKTTKESSIQQLRRQMGIVFQDFKLLADR
                     NVFENVALALRIVGTRTKDIRPMVYEALALVGISQKRLSMPYTLSGGEQQRVAIARAM
                     VHNPYLLLADEPTGNLDPENAKEVFSIFKEINARGTTIVMATHNPDFYLNSPFRRLTL
                     NHGTLLNRDIL"
     gene            10433..11179
                     /gene="scpB"
                     /locus_tag="PLT31_01285"
     CDS             10433..11179
                     /gene="scpB"
                     /locus_tag="PLT31_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HPW93796.1"
                     /translation="MDQENLKEKVKVINEEQVLPEEEIDESSDLPKIESSEDLARIIQ
                     AIVFASPEVVTLKKLREILGDFLDSRSVSDALLAANDSLNKIQSPFEIVEHAGGYRFR
                     TRAKYYPWVRKLFPDITARRLSQAALETLAVVAYQQPITRAAIEQVRGVSCDAPLRSL
                     LEKKFIALGARAETVGNPYTYITTAEFMKYFGINRIPEDLPRLREFEDLISAGMLIPQ
                     YERPDSLMEPKGISDEGVNPDQVELTMGDP"
     gene            11176..13779
                     /gene="mutS"
                     /locus_tag="PLT31_01290"
     CDS             11176..13779
                     /gene="mutS"
                     /locus_tag="PLT31_01290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPW93797.1"
                     /translation="MTPLMRQYNEIKKEHPGCVLFFRMGDFFELFDTDAELAAGILGI
                     TLTSRNNGDAASTPLCGFPHHAAERYIPKMVAAGHRVAICEQIEDPKLSKGIVKRAVV
                     EIISAGTAMNEGNLVEKESNYLSVMLPLSDDQVAFAFSDVTTGFFAVSVSGIQAFESE
                     FSKRMPKEVLIQAESKIPKPIQDLIQAESILVTPLESSLFEETEARRLLFEHFDVENL
                     ESLGLEGKIAETQVAGAMLAYLVDQKKSELSHITHLEFLDLGDYMTLDSATQRHLELV
                     RPLNADDASSTLCSVLDHTVTAMGGRLLKEWLTHPLVKVERIEARSSAVAELVKNPIV
                     LDELKKDLQAILDMERLMGRVGTGRANARDLAGLGHSLERSISVSATLASLESQTFKR
                     LYQVLELAQGRGKEILNYLNDELPLTVREGGMIRPGASAELDEMNAEIKDKRQWISSL
                     ETREKERLGITTLKVGYNKVFGYYIEVTRAQTSKVPPEYIRKQTTVNSERYITPEMKE
                     CESIISNAEVRIHAEEYKIFCELRDKVNSWRGELQQIAEAISETDSLYSFAVAARRYN
                     YTRPKVHEGNGIEIKGGFHPVIVAVNPDIQFVPNEVSLSSTQERLMLITGPNMAGKST
                     YLRQTGLIVLMAQIGSFVPAESADIGVVDRIFTRVGASDRLSRGLSTFMVEMVETANI
                     LRNATPKSLILLDEIGRGTSTFDGLSIAWAIVETLHGAETDIEAPMTLFATHYHELTS
                     LVDSLPLAGNYQVLVQEKNDKLVFLHQIAKGACDSSYGIHVAEMAGLPTQVVRRARRI
                     LLRLEKHKIDPSDTTVEKKKKETPQVDIFAPPDENTNLICHELARVKPEEMTPLQALQ
                     FLSELKEHYHL"
     gene            13776..14279
                     /locus_tag="PLT31_01295"
     CDS             13776..14279
                     /locus_tag="PLT31_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93798.1"
                     /translation="MSSIDFTALQEFVFSQKMTPDNTVHGIDHWTQVESNALLLSRHT
                     GADETVIRLFSIFHDSRRFDDGYDSEHGPRAAEFLMQIRGDVFVLDDARFDLLYHACY
                     YHTKEHSSGDKTIDTCYDADRLDLGRVGIHPKPEKMATAIGQKIVKKAKDVPLSEMRQ
                     WLNKLTL"
     gene            complement(14269..14781)
                     /locus_tag="PLT31_01300"
     CDS             complement(14269..14781)
                     /locus_tag="PLT31_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPW93799.1"
                     /translation="MMNIFEKIEKSGLKLPCCPKPLAAYTPAIRVKNTIYVSGQLPIS
                     EDSMPTGIVPTNITVTNAQNAAATCFLNALSAALSMISKTEDLQLVQIQGFVQCSSDF
                     KDIPLVINGASELALHIMEDKGVHARTAVGVASLPKDAPVEIACIFDVINTTAPNYSG
                     RYEWLESTKE"
     gene            14834..15493
                     /locus_tag="PLT31_01305"
     CDS             14834..15493
                     /locus_tag="PLT31_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93800.1"
                     /translation="MFGEIDFMLLPAWISKLEKKIGWLQIPQLGLILICLQIIGFILI
                     LRRPEMAQILYLDHAAILKWEIWRLFTFIAIPLTSDFLMLFLWWFLYYIFKSLRVQWG
                     DFKLTLYFLIGWIGTVLGSFVFNISVDSFMLMLSTFFFAIATLNPEYEVLFFFILPIK
                     MKWIALFTGVVLFLQQAFFGAWQMTFSLIFTCSNYILFFGPHFYRLLRDEMKKRRENS
                     L"
     gene            complement(15695..17953)
                     /locus_tag="PLT31_01310"
     CDS             complement(15695..17953)
                     /locus_tag="PLT31_01310"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HPW93801.1"
                     /translation="MLFNLDMIQSVYSKIPSRVEEARRQLKRPLTLSEKILYSHLVEG
                     SQNQVFSRGSDFAEFNPDRVAMQDATAQMALLQFTTAGKPQTAVPSSVHCDHLITAKN
                     GVEQDLPTALQENKEVYDFLSSVSAKYGIDFWKPGAGIIHQVVLENYAFPGGMMIGTD
                     SHTVNAGGLGMLAIGVGGADAVDAMVGLPWELKYPKIIGVKLKGALQGFATPKDIILA
                     LAGILTVKGGTNAIIEYFGEGARQISATGKATIANMGAEVGATCSTFSYDDSMARFLR
                     ATGRSAVADAADAIADHLRCDAEIEANPELYFDRVIEIDLSTLKPHYNGPFSPDRSFV
                     INDMEKSLQEFDSPETVSAALIGSCTNSSYEDLYQAAGLIKQALEKGLKPKAPLLINP
                     GSEQVRYTAERDGLMDLFKAFGATIMTNACGPCIGMWDRLGADKKEKNSIVHSFNRNF
                     AKRADGNPNTHAFVASPQMAVIAALSGSLLFNPETDTLINEEGKAVKLTPPPSVELPA
                     NGFDVKDPGYAAPAKDGSSISIMVSETSKRLQLLKPFTEWNKKDIQGAPLLIKAKGKC
                     TTDHISMAGPWLKFRGHLENISGNMLIGAVNAFNGETNKVKSKSGEYMEVPALAKIYR
                     DQGTGSIVIGDENYGEGSSREHAAMEPRFLGVKAVIVKSFARIHETNLKKQGMLALTF
                     TNPADYDKIQEGDVFDILGLEQFDPKASLTLVAHHADGSIDKISLSHTYNEQQWKWFN
                     AGSALNLISAKA"
     gene            18147..>18905
                     /locus_tag="PLT31_01315"
     CDS             18147..>18905
                     /locus_tag="PLT31_01315"
                     /inference="COORDINATES: protein motif:HMM:NF013695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HPW93802.1"
                     /translation="MKKRLLHKKQKREALFEKKDILALKAIEVRALKAGIWFNAIMAI
                     CGYVGYYFADSYALAFDGNFSLVSALSCYIAMRITKRKDVSNAEYPYGYYSYENVYAL
                     LQGILLIGITFYAILTGVTNIFHFVKGETIETLKIAPLLLYTVTMLFFCAAVWLYYRQ
                     QLERSGGQSPVLKTETVAAFMDTFITFGTGVALILVALIPENHSLRFLSYIGDSVIVI
                     LISILIIREPIRIVSQSFFSLVGRTVSNKKVRRRV"
BASE COUNT         5591 a         3379 c         3894 g         6041 t
ORIGIN      
        1 ttgcatacat catcaggctt aattacattg cccaaagatt tactcatggt acggccttgg
       61 ccatccatga ttttagggtg gatatagact ttcttaaatg gaatagttcc catattttct
      121 tggctgaaaa taaccatgcg agcgacccaa agggtaatga tatcgcggct ggtaaccaag
      181 acagaagtag ggtagtactt tttgagttct ggggtagatt ctggccagcc catggtacta
      241 tgaggccaaa gggcactgga gaaccaagta tctaaaacgt cagggtctct ttctacgcga
      301 tgcccttcga tttcattttc tttcaaatcg gattcttgag agcaaattaa ccagccacct
      361 tgttcttttt tagaataaaa gacgtctttt ctattggcaa aggctttttt caagtcttct
      421 tcagaggctg tggcacagta ccaaatagga atctggtggc cccaccataa ctgacggcta
      481 atgcaccaat ctcttttttc accaagccaa tcaaggtagt ttttggcata gcgttcagga
      541 ataatttcaa tttctttatt ttgaacggca ctcatggctt tttcggcaag agagtccatt
      601 tttacaaacc actgatcgct taagtaaggc tcaatggccg ttttagaacg gtcgctgtga
      661 cccacttgca tttcgtggtc ttctactttt atgagtaagt tttgttcttc aagagcttga
      721 acaacggctt cacgagcttt tttgcctttt aatccacaaa agactcctgc gttttcatta
      781 agagaaccgt cgtcattcat gatgttgatc ataggaagct tgtgacgtaa accagtggca
      841 tagtcattag gatcatgagc tggagttact tttacagaac cagtaccaaa ttctggatcc
      901 actaaaagac cgtctgcaat cactggaatt tcacgatcag taaaaggcac tttgagcatt
      961 ttaccaatgt atttttgata gcgttcatca tctggatgaa cggcaagagc agtatctccg
     1021 agaatcgttt caggacgtgt ggtagacaca ggaataaagc cagaaccatc ggcgagaggg
     1081 tatttgaatg tccagaaaaa acctttcaca gtttcgtaat agatttcatc atcagcgacg
     1141 gctgtttgga gatgagtatc ccagttgact aggcgtttac cacggtaaat taagcctttc
     1201 tgaaacaaat taaagaatgc gtgacgcact gcttttgcac aaatggggtc tagagtaaaa
     1261 cgttgacgat cccaatcaca gctagaacct aagcttttta attgcttagt gatgcgagct
     1321 tcgtattctt ctttccattt ccaaatacgt tcgacaagag cttcgcggcc aatgtctctg
     1381 cgagtctttt tctcatcttg gagtaagcgt ttttcaacaa cggcttgagt cgcaatgcct
     1441 gcatggtctg tacctggaat ccataaggta tcacaacctg tttttctacg gtagcgaaca
     1501 agaatatctt gaatcgtatt gtttaaagca tgaccgagat gtaaggcccc agtgacgttt
     1561 ggaggcggga tgactactga aaaaggagtt cctttgccag aaggggtaaa actttttttc
     1621 ttattccact cgctatgcca gcgagcttct acttcttggg gattatatcg tgtttccatc
     1681 atggaaaaca aatgtagaaa aaaatgagga ttttgctatt acttcaaagc ggcaattgca
     1741 tctcctttgc ttttatttaa atgaatttcg agtggttctt gttttaaatc ccaaatgtga
     1801 atcaaaggcc aaagttctgg atagctcgtg agattttcag aacatcactt tgtgatcctt
     1861 ggctttaaga ggaaaactta gggtgaattg ctttcgaaaa aatacatttt cattgaaaat
     1921 gggtgactta aagcacgcct taagcttcga gcttgctgat tttactttaa gttttcattt
     1981 ttgcgaaagg tcgttcctaa aaaaaactaa attatagagt atggattcaa ttcaaatgac
     2041 atgtccccat tgtgggttga gtatggacgt tttactacga ggaaaaccgt cgtctatgat
     2101 tgtttttatt tgttctcgct gtaaatcacc gttaatgcgc tatgagggag aaatctttga
     2161 attagatcgt gaagaattta attctctacg taaaaaatta tcaagagttt tagatgcatt
     2221 agttgaggaa tcttctgata ttaaacgaga agggcttgct gaaataccgc tttggaattc
     2281 agatgattct attacctcag aagcggtaga cgaaatgctt aagctcttag aagaaagtaa
     2341 agatgttgac gattttataa accagatctg attatgtgga gtttttttct tgcttcctta
     2401 gtcgtagcac tagttcctgg acccgacaat atatttgttc tttctcagag cgcttctttt
     2461 ggtagaaaag caggcttagt tgttattttg ggtctttgca ctgggatttg cattcaaata
     2521 ttattcttag tgacgggttt gtctttaatt cttgtgaatt ctcctaaagc attttttgtt
     2581 ttgcagtgcc taggtgcttg ttatctttta tacttggctt ttctttcttt tagggttcaa
     2641 aagggcgtta tctctgtaga aaaaacgaaa gctctatcgc cttttaagct ttatcgtaga
     2701 ggggctttta tgaacttaac aaaccctaaa gcggtgattt ttttactcgc ttttttgccg
     2761 cctgcagtca atacctcgct ctctttatca cccacgactc agattattgt tttagggctt
     2821 ctttttatcc tttcggtttg gattgtcttt ggggcttttg ctttttttgg agggtggatt
     2881 tatcactttt ttagtagttc tccaaaggcg aatctttact taaatagggc ggcgggggct
     2941 gtttttattt tcctagcgct ctctcttttt atatggtagt ccgtttgtat gtttgattcc
     3001 tctcttttga aaatagaata tcctgatctt ccagtagtag aaaaaaaaga ggaatttctc
     3061 tctcttctaa aaaaccattc tgtggttatt gtacaggccg atacagggtc tggtaagtcg
     3121 actcagcttc ctaaatttat attagaatca ggcattgctg cacaaggtcg tataggtgtt
     3181 actgaaccaa gacgtttggc ggctctctct attgcagatc gtcttcgaga agaattaaaa
     3241 gacgaatctc ttgtcgctgc gcgtattcgc tttttagaag aagggtctgc caaagctcct
     3301 attaaggtca tgaccgatgg tattttgttg caggagtttc gccgagatcc tctttttaag
     3361 cagtactctg tggtgatgat tgatgaagca catgaacgtt ccttaaacat cgacatttta
     3421 cttggtatat ttaaaaacgt tttagaaaaa aggcctgatt ttcgtttgat tattgcgtct
     3481 gcgacgctcg atggagaatt atttgctgaa ttttatcaga acgctaagat tctaaaagcc
     3541 gaagggcgtt tatatccagt agatgtggaa tatcgttttt taactccaga agaagaaaga
     3601 gaagaaagcg atatcaattt aacggaacaa gctcgagacg ctattcttga gctactctct
     3661 agagcaagag atcacttgct ttgcttttta ccgacagaaa gagatattca agatttagct
     3721 atagaattac aaaaagattt agattcaaac acctttgaaa ttcttccgct ctttggtcga
     3781 atgagcccca aagaacaaag aaaagtattt caatcgacgt caaaaacacg cgttgtatta
     3841 tccaccaata ttgcagaaac ttctttaacg attccaggaa ttgcttatgt agtcgattct
     3901 ggccttgcaa gagtttcaag atataacgct caagcgagaa tccaaggttt acctattgag
     3961 gccgtttctc aagcgagtgc ccgccagaga actggtcgtg ctggtcgtgt gaaaccaggc
     4021 acttgtattc gattgtacga tgaaaaaagc tttagtgaaa gagatctttt tacagagcct
     4081 gagattcgtc gttcgaacct cgcgaacgtt gttttgcaat tgcagagttt aggtttagag
     4141 gttgaaaatt ttccctttat acaaccgcca ccgagatccg cttttagagg agcgtataaa
     4201 acccttcatg aattaggggc gttgagcatt gcagaagcag gggcttcggt cactgatttt
     4261 ggtcttgaaa tggcaaaatt gccgatggat gtagctcttt cggcagtgct attaaaggcc
     4321 cgagaatttt ctgttttaca gccagcggtg attatctctg cggcgttatc gattcaagac
     4381 ccaagggttt taccttcaga agaaccagaa agaacgcgag ctcgagaggc gcatcgtcgt
     4441 tttggaggcc ataagagcga ttttttactt tacctttcga tttggaatgc gtttagtgaa
     4501 gatttcgaaa atacctcttg gaataaacta aggaaattct gcgaaaaaca ctatttgcat
     4561 tttttacgcg ttcgagaatg gattgatttg tatgaacaat actgtcgttt gttaaaagtg
     4621 caatataaag atcgtgtttg ctctttgaac agttttcatc gagatttttt acacatggct
     4681 ttgcttgcag gctttttagg cggcatcgcg cgccgtgata ttgaaaatag ctgttatcgc
     4741 ttaacgaatg ggcgtgaaac gcatgtgttt ccaggaagcg atctttatgg aaaaagtgta
     4801 gaatggcttt tttctgcaga agttcgtgaa acgagccgag tctttttaac taaaaatgca
     4861 gaaatccgtt ccgattggat tttaaaagtc gctgaacctt tttgtactcg acgctattat
     4921 gagcccgagt ggaatcgcga gcgcggtttt gtagaaatcc gtgaagaagt ttcttttaga
     4981 ggttttgtca ttgcaggcgg tcgtcgtgta gactatgctc gaatagaccc caaagaatgt
     5041 gcacgtatct tttttagaga agccgttgtt ttaatgaata tggctcgtcc ttttgctttt
     5101 atggagcata atgatcgcgt gattcaaaat cttcatgctc aagaagcgcg tgaacgaaaa
     5161 tttggattag cccctaacga agaaatgcaa gtcgattatt tgatgtctgt agctcctgat
     5221 gtctgttcta taaggacttt gaaaaccttt attttagaac ataccgatgc ggccttacgt
     5281 tttgacccga attattttat tgaacaaagc catcgtgggg tagaatacca tgattctttt
     5341 caaaataaaa tacaatggcc ttccaaaaat caaaagcaag aaattcctac tctcacagaa
     5401 aaagggacga gtattgaatt ttttagaatt gcgggcgagt ctgttcaagg agaattggtt
     5461 tttaatgcat cacaaaacaa tgatgggctt tctttaaaaa ttcctcatac gttaattcca
     5521 tcgctttcac ctgccttttt agcgttacag ctttctcgtt ggcgtcattg gatgctcgaa
     5581 gcgatgattc gagagctgcc aaaagtaaat cgtaaatcag taaaaaatca atttgaagaa
     5641 atcgatgatc atttttgtgt taaattagaa caacatccta atgcttctcc ttttcttgct
     5701 ttgtatgagg cactttctga aatgaaagga ttagatgata ttcatcttaa tttaaatcca
     5761 gagaaggaac atcatcttcg gcttcacttg agctttttca gggaagggta tttagaagaa
     5821 aaaaaggtag aattatcgcc agaatggggc tcttttcgct ttttcaaaga aatacgttcg
     5881 ttgatttctt cgtttggagt cgatgtaaat ctcggagact ttaggtatgg atatcgtctt
     5941 cacgaaaatg ctttgatgat gcctgaagaa tccgattttt ggcaacaaag attaagaaaa
     6001 attgaaaaag gagcttgtca gcaatcggtg atgttgatta tgaatcgtct tgcatttatt
     6061 gaaaatgcag gcttatattc taatgaaaat ccattacctc ataaagacga agtttttaaa
     6121 actttagtaa cagaaccttt tgaacccgat gaattagacc gttttagtca tttgcatttt
     6181 tctcgttcta aaaaagtgaa tgattttaag gctctccaag aacaaacaaa accagaacaa
     6241 gaaataatac gtttgatttt aaatcgaatg acttttgagt ctggattgtt aggtaaagag
     6301 gcttttgttc gatgttatga aacgttaaaa cgcttttgtg agaatactcg cggaaatcgt
     6361 ccagtggacc ccgtttttaa gaagaaggtt ttagcttttc cagagatttc ttctcagtat
     6421 aatcgcttga aatatataga agattggtta gaaagtggaa attcattttc tttagtagaa
     6481 gatccgatgg atttaattca atggaaatcg atcagagagt cttttaggcc ttatttcgtt
     6541 gctcgttata tcaaagaaga cgaacagata aagatgcgtc atctcctttc ggctcttgaa
     6601 aaagagatga aatcctcaaa tgattatttt gaaatatggc ttgaggttca attgctttta
     6661 gaagaatttg aatatttcaa gttcaaaaga agcattcttt ttgaatctga cgctttagaa
     6721 gtagaaaaat catcaatagt gaagctaaaa gggcgttttg gaaagcttta agcttttccg
     6781 agcctcatct tttttctagt ttaggtcgca atgaaatttt ttgttttact tcttagtttt
     6841 tttgccctat ggggttgtga tttttcttgg tttaccaaag atcctgttgt ggtaaccgta
     6901 ggggatgtta gattaaaaga atccgatctt agagccaagg tgacaggctg ggattctctt
     6961 tctaatcgag aacaaattgc ttgggtcgaa gcatggattg atgaagaact tctttatcaa
     7021 gaagccttaa atcttggaat tcatagagaa aagcaaatta aagaacgtct tattttaaca
     7081 gaacgtaaaa ttgtcgttga agcttttatc tcaaaaatga ctgattctct ttcaattgat
     7141 attaaggaag cgaaacgttt ttacgatact catcctgaat tgtttattta cgacaaatgg
     7201 ttgtggtctg gttttatgct ttcctataca gattggaaat gggcaagtag ctattacaaa
     7261 gccaaaaaag aatctgtttt tgattcctta gttcacgaag attttcgcct aaagaaaata
     7321 atttcttttg attctgttct tgaaacacca gattcttgtt tatctacgga ccttagagaa
     7381 ttatctttag ggttacttac agccccaaaa gtttgtaatg gtttcttaaa gagcattgtt
     7441 attacaaatc gtcaggattc aggtgcagtg attccttttg aacaagtaaa agatcaagcg
     7501 catgctcttg ctcaaatcaa taagcaaaaa gaaactttgg ctgttttaaa aactgaatta
     7561 aaaaagaaac gtcctatatt ttctaacccc caattatttt ctgattcatt agatcaaaag
     7621 aggaataaat gatccactgg cgctatttgc ctttaacttt atttttattt gtttttgctg
     7681 ctactccatt ggcagctaaa gagttgcttg aatctattgc agccattgtc gatggaaagc
     7741 ctattatgcg ttccgaggtt ttaggcggtt tatatcagtt aaaaaatagt cctgaagctc
     7801 aatcgatgaa tgaatctgaa cagatgaaat atgttttgaa tcatcttata gacgaaaaag
     7861 tccttttaag tcgcatttcc agagattcga ttgttgtttc tgatgaagaa gtggatcaga
     7921 gagtctcgat gcatttaaat aacttagcgg cttctcaaaa tacagatatt gctaccttag
     7981 aaaaagccat tcgtatgcaa ttagggatta gcgttgctca atatagagac cagctagcta
     8041 aacaaattcg agatcatatt tatgtggctc gtattcgtca aattcacgtg ggcgctatga
     8101 ctcctacaaa aaaagaagtg gtagagtttt ataacgttta taaagattct ttgcctcgtc
     8161 aatacaattg tatttcggta agtcatttac aattgaatat tgagcctggt cagtcaattt
     8221 tggattctgt aaaaaaagac gctttacaat taattgatag cttagaccgt ggaatgaact
     8281 gggaaattct tgccaaacgt tattctcaag atgaatccgc tggaaaaggt ggcgatatcg
     8341 gttttgctcg caaagggact cttgatcctg agtatgaacg agtcatggaa agtttaaaaa
     8401 atggtcaata tgcagatcaa cctgttaaaa cccctttagg atatcatatt gtgaaagttc
     8461 ttgggcgtaa agaagatggc attcgttctg ctcatattct attaaaaaca ataccatctt
     8521 cacaagattc cttaaaagta atagagcaaa ttaataccat tcgtgaagaa attaagacta
     8581 aagaagcttt tgcagaaaaa gcaaagcaat ggagtcaaga taaggaaacc aattttaagg
     8641 gtggagattt aggttggttt cagagaagtg aatttgatac taattatgtg caaaccattt
     8701 caaggctttc agttggagat atctcagagc ctgttttgat tgataatgcc tatcatttat
     8761 tccgtttaga tgatgaaagt caggtacgtt ctttgaattt agaagaagat tatcctaaaa
     8821 tcgaagctct tgctgcaaat caaatggaaa caaaaaaatt agaatcacta gtaaaaaaat
     8881 ggcgccaaga agttcacatc gaaattcgtc tcaaggaata gaagtagccg ctccctacga
     8941 aaaacttgct ccatattaca aaaaaataat ggagcatgta gattatgtag actgggcttt
     9001 ttatttaaat actcttttaa aaaagcataa acgagaacca tcttcgttat tagaattagg
     9061 cgctggaaca ggtttacttg cggagcattt taagcccgtt tctataaaaa aaagaatcac
     9121 tacagattat tcttttgaaa tgattcgtaa ggcttctcca aaaacagcag ggtatcgtgt
     9181 ggcagcagac gcttcatctt taccttttac tggcgtcttt gatatgatgc taatgactta
     9241 cgatgccatt aattacctct caaaatcgaa aatagaagat ttatttaaag aagtctttcg
     9301 cttattatca gaaaaaggtg tttttatttt tgatgtgacg acagaataca acagccttac
     9361 ttattttgaa gatgttgttg atgtagaaga atgggccaat gattttatta tacgtcgttc
     9421 ttggtatgag tctcgatcac tcaaacaata caatcagttt gattttttta tacttcaaaa
     9481 agacggttct tatttaagag aaacggaaaa acatgaacaa accattcatc ctgtttcttt
     9541 ttttataaaa gcagcagaga aagcgaagct cgaagtggaa ggtgtttatg ccgatttcac
     9601 tacggaaaca gatctttctt atgcagaacg agtacatttt gttatatcta agaaaaagaa
     9661 tacaaaagca aggccttaaa atatgattca tttttcgcat gtcactaaaa cttacgaaga
     9721 caactggaaa gccctttcag atgtgacttg tcggattcat aaaggtgaat ttgtcttttt
     9781 aacagggcat tcaggtgctg gtaaatcgac tttgctaaaa atggtgtata tggacgagcg
     9841 tccagatcat gaccgtggtg gtcagattat ggtcaagttt acacaagatt cagtttacga
     9901 tagtaaaacg acaaaggaat cctctataca gcagttaaga cgccagatgg gaatagtgtt
     9961 tcaggatttt aagctattag cggatcgtaa tgtttttgaa aatgtggcct tagctcttcg
    10021 tattgtgggg actcgaacaa aagatattag acccatggtt tacgaagcac ttgctttagt
    10081 tggaattagt caaaaacgct tatctatgcc ttatacttta tctggcggag agcagcaacg
    10141 agtggctatt gcaagagcga tggttcataa tccttatttg cttttagctg atgaaccgac
    10201 aggtaatttg gatcctgaaa atgcaaaaga agttttttcc atttttaagg aaattaatgc
    10261 tcgaggcaca acgattgtga tggcaacaca taaccctgat ttttatttaa atagcccttt
    10321 taggcgatta actttaaatc atggaacatt attaaatcga gacatccttt aacgccagct
    10381 tttttgaaga actcgtttac aaaaaggctt tggttttctt aaattggttt atatggatca
    10441 ggaaaattta aaagaaaaag taaaagtgat taatgaagaa caagttctcc ctgaagaaga
    10501 gatcgacgag tcttctgatc ttcctaaaat tgaaagtagt gaagatcttg ctcgcattat
    10561 tcaagcgatc gtttttgctt ctccagaagt ggtgacgtta aaaaagctaa gagaaatttt
    10621 aggcgatttt ttagattctc gctccgtttc agatgctctt ttagcggcaa atgattcttt
    10681 aaataaaatc caatctcctt ttgaaattgt agaacatgct ggtgggtatc gttttagaac
    10741 aagagccaag tattaccctt gggttcgaaa attgttccct gatattaccg caaggcggct
    10801 gtctcaagcg gcactagaaa ctctagcggt agtggcttac caacagccga ttacacgtgc
    10861 tgccatagaa caagtgagag gggtttcttg tgatgctcct cttcgtagtt tgcttgaaaa
    10921 gaaatttata gctcttgggg ctcgtgcaga gactgtgggt aatccataca catacatcac
    10981 aacggctgaa ttcatgaagt attttggaat caatcgtatt ccagaagact tacctcgttt
    11041 acgcgaattt gaagacttaa tttctgcagg aatgttgatt cctcagtatg aacgtcctga
    11101 ttctctcatg gaaccaaagg gtatatctga tgaaggggtg aatccagatc aggttgagtt
    11161 gacgatggga gacccatgac ccctttgatg cgccaataca atgaaattaa gaaagaacat
    11221 ccaggctgcg tcttgttttt tcgcatgggt gatttttttg aactctttga tacggatgca
    11281 gaacttgcgg ctggtatttt aggaattacc ctcacttcta gaaataatgg cgatgcggct
    11341 tccactcctc tatgtggttt tcctcatcat gcggcagaac gctatatccc taaaatggtg
    11401 gctgcagggc atcgtgtggc catctgtgag caaatagaag acccaaaact ttcgaaaggg
    11461 attgtcaagc gtgccgtcgt ggagattatc agtgcgggca ctgcgatgaa tgaagggaat
    11521 cttgtcgaaa aagaatcgaa ttatttaagt gtgatgcttc ccttaagtga tgaccaagtc
    11581 gcttttgctt tttcagatgt tacaacgggt ttttttgctg tatcggtttc tggtattcaa
    11641 gcttttgaaa gtgaattttc taaaagaatg ccaaaagagg ttttgattca agccgagtca
    11701 aaaattccaa agccaattca agatttaatt caagcagaat ctattctcgt gactcctctg
    11761 gaatcttctt tatttgaaga aacagaagct cgtcgtttat tgtttgaaca ttttgatgtt
    11821 gaaaatcttg aatctctagg tttagaaggt aaaatagcgg agactcaagt cgctggggca
    11881 atgcttgctt atcttgtgga tcagaaaaaa tccgaattat ctcatataac gcatctcgag
    11941 tttttagatc tcggtgatta catgacttta gattcagcga cgcagcgcca tttagagtta
    12001 gtgcgtccat taaatgcaga cgatgcctct agtacgcttt gttctgtttt agatcatact
    12061 gttacggcta tgggagggcg tctcctcaaa gaatggctca cgcaccctct agtgaaggta
    12121 gagcggattg aagctcgtag cagtgctgtt gctgaattag taaaaaatcc aatcgtatta
    12181 gacgaattaa aaaaagatct tcaagctatt ttggatatgg aacgcttgat gggacgtgtt
    12241 gggactggaa gagctaatgc tcgtgattta gcgggcttag gtcattcttt agagcgttct
    12301 atttctgtat ctgcgacact tgcttcctta gaatcccaaa catttaaaag gctctatcaa
    12361 gttcttgaac ttgcacaagg tcgtggaaaa gaaattttaa attacttaaa tgacgagctt
    12421 cctttgacag tgcgcgaagg cggtatgatt cggccaggag cttctgctga attagatgaa
    12481 atgaatgccg aaatcaaaga caaaagacag tggatttctt cattagaaac aagagaaaaa
    12541 gaacgacttg gtattacaac gttaaaagtt ggttataaca aagtctttgg ctattatata
    12601 gaagtcacaa gagcacaaac ttcaaaagtc ccgcctgaat atattcgaaa gcaaacgacc
    12661 gttaattcag aacgctatat cactccagag atgaaagaat gcgaatcgat tatctcgaat
    12721 gcggaagtgc gaatccatgc cgaagaatat aaaatctttt gtgaattacg agacaaggtc
    12781 aactcgtggc gaggagagct tcaacaaatt gcagaagcca tttctgaaac agattctttg
    12841 tacagttttg ctgtagcggc aaggcgttat aattatacaa ggcccaaagt acatgaaggc
    12901 aatggaatag aaattaaagg cgggtttcat cctgtcattg tggctgtcaa tcctgatatt
    12961 caatttgtgc caaatgaggt ttctttaagt tccacgcaag aacgcttgat gttgattaca
    13021 ggccctaaca tggctggtaa atcgacttat ttaaggcaga ctggtttaat tgttttgatg
    13081 gcccaaatag gttcttttgt ccctgcagaa tcagccgata ttggtgttgt tgatcggatt
    13141 tttacacgtg taggcgctag cgacaggctt tcaagaggcc tttccacttt tatggtggaa
    13201 atggtggaga ctgctaatat tttaagaaat gcgactccta aaagtttgat cttattagat
    13261 gaaattggtc gaggcacgag tacttttgat gggctttcta ttgcatgggc gattgtagaa
    13321 actcttcacg gtgcagaaac tgatattgaa gcaccaatga ccttatttgc aacgcattac
    13381 catgaattga caagccttgt agattctctt cctttagcag ggaattacca agtccttgtt
    13441 caagaaaaaa atgataaact agttttttta catcaaattg caaaaggggc atgtgattca
    13501 agttatggta ttcatgtggc tgaaatggct ggtttaccaa cacaagtcgt tcgtcgtgcc
    13561 cgtcgtatcc ttttaagatt agaaaagcat aaaatcgatc caagtgatac gaccgtagaa
    13621 aagaagaaaa aagaaactcc tcaagtggat atttttgctc ctcctgatga aaatacaaat
    13681 ttaatttgtc atgaactagc gcgtgtcaaa cccgaagaaa tgactccttt acaggcctta
    13741 caatttctat cggaattaaa ggaacattat catctatgag ttcaattgat tttactgcat
    13801 tgcaagaatt tgttttttct caaaaaatga ccccagacaa tacggttcat ggcattgatc
    13861 attggacaca agtggaatca aatgccttat tgctttcgcg ccatacaggt gctgatgaaa
    13921 cagtaattcg acttttttcc atttttcatg attctcgtcg ctttgatgac ggttatgatt
    13981 ctgaacatgg cccgagagcg gcagagtttc tcatgcaaat tcgaggggat gtttttgttt
    14041 tagacgatgc tcgttttgat ttattatacc atgcgtgtta ttatcatact aaagaacatt
    14101 caagtggtga taaaacgatt gacacctgtt atgatgcaga tcgtctcgat ttaggtcgag
    14161 taggtataca cccaaaacca gaaaaaatgg caacagcaat tggtcaaaag attgtgaaaa
    14221 aagcaaaaga tgtgccatta tctgagatga gacaatggct caataagctt actctttagt
    14281 ggattcgagc cattcgtagc gcccgctgta atttggggca gtcgtattta taacatcaaa
    14341 gatacaggct atttcaacgg gggcgtcttt gggtaaagaa gcgacaccta cagcagttct
    14401 tgcgtgaacg cctttgtctt ccataatgtg aagagctaat tctgaagccc cattgatcac
    14461 gagaggaata tccttaaaat cagaagaaca ctgcacaaaa ccttgaattt gtactaattg
    14521 caaatcttct gttttagaaa tcatagaaag tgctgcagaa agagcattta aaaaacacgt
    14581 ggcagccgcg ttttgagcgt ttgtgacagt aatatttgta ggaacaatac ccgtgggcat
    14641 tgaatcttca cttataggta attggccaga aacataaata gtgtttttga cgcgaatagc
    14701 tggggtatat gcagcgagag gctttgggca acagggtagt tttagtcctg atttttcaat
    14761 tttctcaaaa atattcatca taaggggaac tcgctttgtt atcttttaat aagatagcat
    14821 tattttatgg ataatgttcg gggaaattga ttttatgctt ttaccagctt ggatatccaa
    14881 attagagaag aaaatcgggt ggttgcaaat accgcagcta ggtttgattc ttatatgctt
    14941 gcagatcatt ggctttattt tgatcttgcg caggccagaa atggctcaga ttctttattt
    15001 ggatcacgct gcaattctta aatgggaaat ctggcgttta tttacgttta ttgcgattcc
    15061 tctcacgagt gattttctta tgcttttttt atggtggttt ttatactaca tatttaagtc
    15121 tcttcgagtg caatggggag attttaaact gactctttat ttcttgatag gttggatagg
    15181 aacagtcctt gggagctttg ttttcaatat ttcagtcgat agttttatgt tgatgctgag
    15241 tactttcttt tttgcgatag ccacactcaa tcctgaatat gaagttttat tctttttcat
    15301 tttaccgatt aaaatgaaat ggattgctct ctttactggg gttgttttat ttttgcagca
    15361 agcttttttt ggtgcttggc aaatgacctt ttctttaata tttacttgta gtaattatat
    15421 cttgtttttt ggacctcatt tctatcgatt acttcgagat gaaatgaaaa aacgacgaga
    15481 aaatagcttg taattttata ttacacgctc gcatctctta ttatttttct tacaacgtag
    15541 ttccccttct aataattgaa attatttgaa aaatactgaa ctccttatcg aattaatact
    15601 tatagaataa ttctatttgc ctttcagatt aaaataagca taaaaataac ccgcttagaa
    15661 ataagcgggt taaaaataga ttctctagat acaattaagc ttttgcagag attaaattca
    15721 atgcactacc tgcattaaac catttccact gttgctcatt gtaagtatga gaaagagata
    15781 ttttatctat cgaaccatcg gcatgatgag caactaaagt aagactagct ttaggatcaa
    15841 attgttctag cccaaggata tcaaatacat cgccttcttg aatcttatca taatctgctg
    15901 gattagtaaa agtcaaagcg agcatccctt gttttttgag gtttgtctcg tgaattcgag
    15961 caaagctttt cacaataacg gctttaactc ctaaaaagcg tggttccata gcagcgtgtt
    16021 cacgactgga gccttcgcca tagttttcat caccgatgac gatagagcct gtgccttgat
    16081 cacggtaaat ttttgctaaa gcaggcactt ccatgtattc gccagatttg ctcttgactt
    16141 tattggtttc gccattaaag gcatttactg cgccaatcaa catgtttcca gaaatgtttt
    16201 ctaagtgccc acggaacttg agccatgggc ctgccataga aatatggtct gtggtacatt
    16261 tgccttttgc tttaattaaa agaggagccc cttgaatgtc ttttttattc cactcagtaa
    16321 aaggctttag aagttgcaag cgtttgcttg tctctgacac cataatagaa atggaagaac
    16381 catcttttgc aggagcagca tagcctgggt ctttcacgtc aaaaccattt gcaggtaatt
    16441 ctacagaagg aggaggcgtg agttttacgg ctttcccttc ttcattgatc aacgtatcag
    16501 tttcagggtt aaataataaa gaaccactta aagcggcaat gacagccatc tgtgggcttg
    16561 cgacaaaggc gtgagtattt gggttaccat cggctctttt ggcaaaatta cgattaaaag
    16621 aatgaacaat ggagtttttt tcttttttat cggcaccaag acgatcccac ataccaatgc
    16681 aaggaccaca agcatttgtc ataatggtcg ctccaaaggc tttgaaaaga tccattaaac
    16741 catcgcgttc agctgtataa cgtacttgtt cagaacctgg atttattaaa agaggggctt
    16801 taggttttaa tcctttttct aaggcctgtt taattaaacc agcagcttgg taaaggtctt
    16861 catagctaga gttggtgcaa cttccaatca aagccgcact cacggtttct ggagagtcaa
    16921 attcttgcaa tgacttttcc atatcgttaa ttacaaaaga gcgatcaggg ctaaatggac
    16981 cattgtaatg aggttttaag gtcgataaat caatttcaat gactcgatca aaataaagtt
    17041 caggattcgc ttcgatttcg gcgtcgcagc gcaagtggtc tgcaatcgca tcggcggcat
    17101 cggccacagc actacgtcca gtcgccctta agaagcgagc catcgaatca tcataactaa
    17161 aagtagaaca ggtcgctccg acttcggcac ccatattggc aatcgtcgct tttccagtcg
    17221 cagaaatttg acgagcgccc tctccaaaat attcgataat ggcgtttgtg ccgcctttta
    17281 ctgttaaaat gccagccaaa gctaaaataa tgtctttagg agtggcaaaa ccttggagag
    17341 ccccttttaa tttcacacca atgattttag ggtatttgag ttcccaaggt aagccgacca
    17401 tagcgtctac ggcatcggca ccgcccacac caatcgcaag catccctaaa ccaccagcat
    17461 ttacagtatg ggagtctgtt ccaatcatca taccgccagg aaacgcgtag ttttcaagaa
    17521 cgacttggtg gataatccct gcaccaggct tccaaaaatc aataccgtat ttagctgaaa
    17581 cagaagataa gaaatcataa acttctttgt tttcttgaag agccgttggc aaatcttgtt
    17641 ccacaccgtt ttttgcagta attaagtgat cacaatgaac agaagagggt acagctgttt
    17701 gtggcttacc agcggtggta aattgaagaa gtgccatttg ggctgtagca tcttgcatag
    17761 ccacgcggtc tggattaaat tcagcaaaat cagaaccacg agaaaaaact tggttttgag
    17821 atccttccac taaatgagag tataaaatct tttcagaaag agtgagagga cgttttaatt
    17881 gacgacgagc ctcttcgact cgagatggaa tttttgaata aacggattga atcatgtcta
    17941 aattgaatag cattagtacc tctttttttt aaaaggtaga aaaacttgaa aatcctttca
    18001 aattttttaa ttaattttag cttgtctctc taagctgcaa tacatttgta ttttgctttt
    18061 ggagtcattt tatttttaaa gatgtcaaat tataaaaaca ccagactcaa atgtcatgaa
    18121 actttctttt tactgagact tcacctgtga aaaaacgctt attacacaaa aaacaaaaaa
    18181 gagaagccct ttttgaaaaa aaggatattt tggctttaaa ggccatagaa gtaagggctt
    18241 taaaagcagg aatctggttt aatgccatta tggcaatttg tggttatgtg ggctattatt
    18301 ttgccgattc ttatgccctt gcctttgacg gaaactttag tttagtctct gcgcttagtt
    18361 gttatattgc catgcgcatt actaaaagaa aagacgttag caatgccgaa tacccttatg
    18421 gatattacag ttatgaaaat gtctatgctc ttttacaagg gattctttta attgggatta
    18481 cattttatgc cattctcaca ggggtcacca atatcttcca ttttgtgaaa ggcgagacta
    18541 ttgaaacatt aaaaatcgcg ccccttttgt tatatactgt aacgatgtta ttcttctgtg
    18601 cggccgtgtg gctttattat aggcagcaac tagagcgaag tggaggccaa agcccagttc
    18661 tgaaaacgga gacggtcgct gcttttatgg atacttttat cacttttggt acaggggtgg
    18721 cattgatttt agtcgcttta ataccagaaa atcattctct tcgctttctc tcctatatag
    18781 gagattcggt tattgtgatt ttaatatcga ttctcattat ccgagaaccc attcgtatag
    18841 tctctcaatc cttcttttcg cttgtgggcc gtacagtatc gaataaaaaa gttagaaggc
    18901 gtgtt
//
LOCUS       DAOOBB010000013        18801 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_3903_length_18801_cov_5.119279, whole genome shotgun sequence.
ACCESSION   DAOOBB010000013 DAOOBB010000000
VERSION     DAOOBB010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 18801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18801
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3903_length_18801_cov_5.119279"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..2401
                     /locus_tag="PLT31_01320"
     CDS             65..2401
                     /locus_tag="PLT31_01320"
                     /inference="COORDINATES: protein motif:HMM:NF027477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4859 domain-containing protein"
                     /protein_id="HPW93803.1"
                     /translation="MTISLLKKSAMLLLSVGAVLGAVAAEGDAWLWPAYSPTLNYNFK
                     DQGKTYTMPTKNHDDCGSEVVGKIDDRWWTFQWGSTKNSLVTDAAIRPMLERFNEDFN
                     YIRDTMGWPPDSRVRDGYRSAIYLYGSIPCSGSTNPEDKGGWQSWVGGYPAVVASYYP
                     VYSFDPSCPYNDKVSQQGAMIHEGIHSLLATMGGASHVHWFQEGGNTWLQQEMEARRG
                     GNYTGMGFLNAGPLIAPFVPIESYSGWLLDGSFGGPGAEGVDVRNGSQQLCNWRNMLG
                     GIQYGNLFPTFLAEWVNPGAIPWIWTYTKDRSKYILHTMSDTLGDAQIRRLIMEYRAK
                     LAMLDMKKWSNEMKNLLNQNFGTSIACEWEPCAATPASWKMTPYVVTTESNGVLTPEN
                     RTTPGWSGANVIPLTVSGSTVKLELQPIGDNMSLQICYRATDGTPVYSEPVMGNAVAT
                     LKLTKTPQSNVVFAVVANTDYKYLGDDTRKKHHDYRLKLTEGVSGAADPHTKWYNNFS
                     LTYDWDAVSHVGSSSSGTGSSSSSAITSSSSVTLTTTEISHNVEVPISSNYASVNVNL
                     ETSKIEQALGITASQIASQLDNGVTFYGVNADGSLYSTSTANAPGHWFDASGNVVSYS
                     DNAIVFSEFDPQSMKVNIGHYPDKVTAGAKYTISQALIYGSKKVVIHFQVSIESKTAI
                     EKSIASTNFNAQKLNISYDRGVIVANYQVPVAGKVKVSLYTAYGALITHLVNKTMDVG
                     SYTKRIDLKAMNIPQGTYLIKFSYPGSSETKVSITAPR"
     gene            complement(3104..3820)
                     /locus_tag="PLT31_01325"
     CDS             complement(3104..3820)
                     /locus_tag="PLT31_01325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HPW93804.1"
                     /translation="MESVYDYSEMKNKLTLLNQRISLACSSCNRSEKEVTLIWVSKFH
                     PQEAVDTAYAIGAREFGENRVQEAIEKFSQKKEGVRCHIIGPVQSNKLKKAALVADVI
                     HSMDSIESLEKLNKYSAELNKVLDVLIQVNTSEEDTKSGLQMSDAKAFLESLPALSHL
                     RYRGLMTIGKNTDLAEDSRAGFSFLRKLREEFVNRDSRFNNFTELSMGMTGDLEVAIE
                     EGATMIRVGTALFGSRQYNA"
     gene            complement(3836..6193)
                     /gene="lon"
                     /locus_tag="PLT31_01330"
     CDS             complement(3836..6193)
                     /gene="lon"
                     /locus_tag="PLT31_01330"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPW93805.1"
                     /translation="MTSNNPFIVDFTKKIPLLPLRDTLLFPSTAMRILVGREVSIQAI
                     DAAHKNDDVVVLALQRDVVEEEVSSTGLYSVGVVARIQNVIPVPNGYFKVLLEGLFTV
                     DITEVTKETFFEALVAPRKMINSNSAKDLKLAQSIIDEFSHYALDQEIQEDFLDTLRQ
                     LDQPIHIYYGILPFIRLSLTEKQAFLEATSLKEVAEKIQSVLYTLKESLQVTKKVRRD
                     VQQKMQQSQKEWIINEQIRLLQEELGDSEFGGAEHVILAKKIKAKNFSAAIQEKLDEE
                     LERMKMMQPTSPEYAVIRNYIDWFLALPYNEYCQTELNLKKVKKELDKKHYGLEKVKE
                     RILEYLAVLKLTGSEKRAPILCLVGPPGVGKTTLVSSIAQAMERSFVRITLGGVRDEA
                     EIRGHRRTYIGAMPGRFIQALRRAKCMNPVILLDEIDKMASDFRGDPASAMLEVLDPE
                     LNQDFTDHFMETGFDLSRVLFIATANTESDIPEALHDRLEIVRLPGYYIHEKKKIASE
                     YLIPEVCERSGVSLGKDIEFSEEIVHKIIREYTREAGVRELERLLSSVVRHRAKELVL
                     NKKSAPTLTDKQISQYLGVPRFLDSRLPSPGRPGLITGLAWTSVGGEILTIECMLLSG
                     KGHLQMTGKLGDVMKESAQIALSLVRARLQRFDVDPSIVRKTDIHIHVPEGAVPKDGP
                     SAGIALTLALLSAFTNRVVDPKIAFTGEVSLTGSLHAIGGLNEKALAALDAGVTTLFL
                     PKDNEKDVAELPAPARKGLKINLKKHIDEIIEELFPQEKKGKKST"
     gene            complement(6233..7522)
                     /gene="clpX"
                     /locus_tag="PLT31_01335"
     CDS             complement(6233..7522)
                     /gene="clpX"
                     /locus_tag="PLT31_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HPW93806.1"
                     /translation="MYKNGKNHPQVACSFCGKPAEQVTKLITGAGVHICNECVAMCHN
                     ILEEEFAREKKTSINQNQKPLPSPSEIKSHLDEFVIGQDQAKIALSVAVYNHYKRLRY
                     DNNRENDVEVEKSNLLLVGPTGSGKTLLAQTLARFLDVPFTIADATVLTEAGYVGEDV
                     ENILVRLLQAADYDVERAERGIVFVDEIDKIARKTANPSITRDVSGEGVQQGLLKILE
                     GTIAAVPPKGGRKHPEQSLVHIKTRNILFICGGAFETLDKVIAQRVNQGGMGFGAHIK
                     TDKENTLGELFKQLEPDDLIHYGLIPELVGRLPVAVALEELDEQALLKILTQPKNALV
                     KQFKKLFELDGIELVFTDDALQEIVNEALARKTGARGLRSVLEKALQKAMFELPGTKT
                     KNLTVTGEMIRHGIESASKKTAVKKSSPSSVNKKKVA"
     gene            complement(7522..8118)
                     /locus_tag="PLT31_01340"
     CDS             complement(7522..8118)
                     /locus_tag="PLT31_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HPW93807.1"
                     /translation="MVIPTVIENTGRGERAYDIYSRLLKERIIFLGTPINDEVANNVM
                     AQLIFLEYENPEKDITIYINSPGGYVSAGLAIYDTMQHIRPNIATICIGNSISMAAVL
                     LAAGTKGKRYALPHSRIMLHQPSGAATGQSTDIQIHAKEIVRTRETLSEIIATHTGRD
                     IKEVKEKTDRDFYMSPEEALSFGLIDEIFHPRRDELIK"
     gene            complement(8131..9390)
                     /gene="tig"
                     /locus_tag="PLT31_01345"
     CDS             complement(8131..9390)
                     /gene="tig"
                     /locus_tag="PLT31_01345"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HPW93808.1"
                     /translation="MTVAIKETSAVLRTIEVSIPQEELKLPFEKKLKKYRQEVTLKGF
                     RQGQVPKAMILSRYGEMIRQETVDEVINQILTEELKKANINPVGRLKVEDSKDDKEND
                     ISFTVVAEVDPVIDIQGYADLGIKVPDVVISENDVKAEMDRLKQMWSKDESVDRAAAE
                     GDVVVGNYLEVIIDGETKEIPEQREFRSLLGESASPGFDEGLKGAKKGETKEINFVYP
                     ADHKDEAYRGKTAQFKVEITDVREVVPPTMDEEFFKQIGVANEDEMRQNIQESLANNK
                     RNAVKTTAVNEAVNKLIEANPFEVPHARVVELIKYTLKRHSQSEEEVEPTEEQMNTLA
                     PEAIREIKKHRILEFVANKENLKATQAMVDARLNELALAYQVDFESLKAHFRQSGRIV
                     SLREELRFEAAADFLVGIRPAAEESSK"
     gene            complement(9422..9502)
                     /locus_tag="PLT31_01350"
     tRNA            complement(9422..9502)
                     /locus_tag="PLT31_01350"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9466..9468),aa:Leu,seq:tag)
     gene            complement(9557..10249)
                     /locus_tag="PLT31_01355"
     CDS             complement(9557..10249)
                     /locus_tag="PLT31_01355"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPW93809.1"
                     /translation="MGKLQGQSSFFKLGPPAQIGEAHQPIVDFLLKEAIGTSILDIGG
                     GCGSYALEMKANGFDPLVVDVDEEALKTAKENGVRVKKVSPNEDLGSELADTVILIEV
                     LEHVDDPKAFLEKAIRAARKRVLFTLPCTNDFTTLFNLGLSYSHIAVTDHLWHFSYSE
                     LENILKSIGKKYRLEVSDYLFPHMVFALMRNCTKFALVGRLAFLPIRVLNRLGFIKKS
                     IPSRFYGIIDMI"
     gene            10302..11537
                     /locus_tag="PLT31_01360"
     CDS             10302..11537
                     /locus_tag="PLT31_01360"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HPW93810.1"
                     /translation="MPQFLRNIKIGVFISFVNLLIQGISFFVQNFVAQNLSTANFGYF
                     GILQTDYSIFSAIADFGMTTLILAFFGDRATKGSLFRNVLQLRLFSTIMTALLMVTFA
                     FTIRRHHPAFKGELILSLGLLFQHAFFDWYFICGKFWKRLFISKLLHTISYAVVMGVA
                     LLYLKLDKIEHIALAMLIAALPAFFFGVTQAFHARLFRITKRTFCFFYLMLKAGIPYA
                     IASLASFAYLPVGLYVADVFASPEFLSAYNFANKIIVLASGFMVHFISSSLINLHQNK
                     DHFLHIKDQLLFTLFIFTASSPLWLFPKEVLQILFFAAPWNDSILAISADCLRILSFS
                     LTFQALRMGLISSLLKDKKTKLYGLFVCIGGGSNIIFCTIAGAFLSNIAIPIFALSGD
                     VVITFILWSYFFKNRSIRW"
     gene            complement(11436..12818)
                     /locus_tag="PLT31_01365"
     CDS             complement(11436..12818)
                     /locus_tag="PLT31_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="HPW93811.1"
                     /translation="MTFDWLAHPESALFFLVGLVALLLASVFKKNLFSPPLLYVLVQS
                     FMFGIAYLKLMPAMTDYQLNTWLVWGGGLFSFLSACYLARLSFRFSNYSLVLPETLSL
                     STEYNWKSHFILSFLALAFFLYGVLGVVSIAGNLVLLTDNPSLWLDGKTSKVLSYAIF
                     FTSSPMVVALFGVASFKSYNPIRWIRNTSKIICLLTIVLSFLTFPSRGVNLMCVGFLF
                     ILANYLYKRITWKMAVVVASLILFFFIGVASVKGQYGEMDVMNNELVDEVALLPYKYI
                     ANNYWNLDYAFNRASDVPEHSWTYGIDAFYGITHLLSIGPGLQASFGWDTPFNESIEK
                     VSSLNTIPYLWDAYKDFGYPGIFLVPFFWGFIFTFLYKKMPMAKTPLSLLFHTTFSFW
                     IILWNFTTGYKQAMYIIWFLFFFFVCTVSSGKGLFSPANRTILKEVTPENERDDDVST
                     ECKNRDCNIG"
     gene            complement(12825..14018)
                     /locus_tag="PLT31_01370"
     CDS             complement(12825..14018)
                     /locus_tag="PLT31_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HPW93812.1"
                     /translation="MDNPSLTELFLRVVNNTLKHFKLAAFIVVVPTIIVFILVVWVIK
                     PTYSSNAIVTPPSSQSSMGNISGLLGSGMGAMGSLLGFSSNDDDANAVWTILNSWEIH
                     NQVIEQFDLVNHYEFDGKFHADLLKTFRKNFSLSANDENMFEITIKDKDYKKATRMVE
                     FVLQKADSAFNYFKTTQARLSREYFESRIALCLHDIDSLQQEFIAFQKDNNFYEPTAQ
                     IEGTLTYLGQIQILKEEVNIERSYEKLRRGEDSKKYDELTKRSRTIDQSLNKVLSGKQ
                     ENVGLLSLKKTPDLVAKYLRMEEEIKVQVALYKVLRQQSEQLRIEEAKTLKNLHVLQP
                     PWENDKKISPRRGLILIFTVFVFTFIALIIGNFLSFVENERQRESHFATEWNKFLKYI
                     CFWHK"
     gene            complement(14033..15088)
                     /locus_tag="PLT31_01375"
     CDS             complement(14033..15088)
                     /locus_tag="PLT31_01375"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HPW93813.1"
                     /translation="MAVEGVIDSSYKLGPGDYLDIMLEQDFFSLQILSDGSIAIEECG
                     IVQIAGKTLAEAREEIVKVASKKYNPEYVFVQLSQMKKFVVSVMGAVKQVGQMVVDPQ
                     TRLSMALQKADNILGTGRKDDVWVIRKGDTLHINCVEIFDKGNFEQDILLEQGDRIYV
                     PFMNTLDMIALIFPGGSRVGVPYDSSSTIAQYFEKAGGDRMHNMGYQSVTIRYPDKTT
                     KVISAKELNHWKVPPQTEIEFSVKTLFVYVGGAVMAMGKIPYESSWHAIDYIAASGVL
                     TQTGTWTQVSVVRGNQEKIKVNVSTDQILPGDYIEIPKSRYETFKDVTMFLASIVSVV
                     STAVIVYINYVNSANSK"
     gene            complement(15324..>15745)
                     /gene="rplQ"
                     /locus_tag="PLT31_01380"
     CDS             complement(15324..>15745)
                     /gene="rplQ"
                     /locus_tag="PLT31_01380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HPW93814.1"
                     /translation="AILRSLATSILEKGLETEQGNRYIRTTLHKAKLAKSVVDRLITY
                     AKKGDLSARREAARFVRDPKVVQGLFDVLGPRYASRNGGYTRVLKLGPNRPGDAAEMA
                     LIGLVEDEIVEKAVKPSQKKAEVKPIDMVEGAAKTAE"
     assembly_gap    15746..15755
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(15815..16789)
                     /locus_tag="PLT31_01385"
     CDS             complement(15815..16789)
                     /locus_tag="PLT31_01385"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HPW93815.1"
                     /translation="MMWKSLQMPRSFQKVETGEDGRYAKFVVEALERGWGITLGNALR
                     RTLLSSLQGAAIVSVKIDGVDKEFTSIPGVKEDVTDIILNLKGIRVKLLSDHDETLRL
                     DMSGDGKITAADIEENPNVVILTPDVHIATLNGNASLSMELKISSGRGYVTADELKDP
                     NAPIGEIAIDANFNPVLKVAMHISDTRVGQRTDFNRLELEVTTDGSIDPEDALAYAAK
                     LLVDHLETFINFEGDLESPEEMEQDEERQRISTLLRMRVDELELSVRSSNCLRMANIH
                     TIAELVRNKENDMLKYKNFGRKSLVELNEVLTSMGLSFGMNVDDYLKD"
     gene            complement(16808..17242)
                     /gene="rpsK"
                     /locus_tag="PLT31_01390"
     CDS             complement(16808..17242)
                     /gene="rpsK"
                     /locus_tag="PLT31_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPW93816.1"
                     /translation="MADEELKEVTAATETVASEEVKVKKGKKRVDIQGIACVNASFNN
                     TIVSITDARGNVVAWGSPGNAGFKGSRKSTPFAAQLASEAAGRKAFELGMRKVDVRVK
                     GAGGGRESAVRALKNAGLEVLSIRDVTGVPHNGCRPKKKRRI"
     gene            complement(17254..17622)
                     /gene="rpsM"
                     /locus_tag="PLT31_01395"
     CDS             complement(17254..17622)
                     /gene="rpsM"
                     /locus_tag="PLT31_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPW93817.1"
                     /translation="MARIAGVDLPRTKTVEYGLTAIFGVGLFTARKVCTQLGIDKDKK
                     CDDLTEEEQGKIRHLLEEEYSVEGQLRAETTLNIKRLQDIGCYRGLRHRKGLPVRGQR
                     SRTNARTRKGPKKTVANKKK"
     gene            complement(17631..17747)
                     /gene="rpmJ"
                     /locus_tag="PLT31_01400"
     CDS             complement(17631..17747)
                     /gene="rpmJ"
                     /locus_tag="PLT31_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HPW93818.1"
                     /translation="MKIKASIKPRCENCKIIRRKGVLRIICSKNPRHKQKQG"
     gene            complement(17772..17990)
                     /gene="infA"
                     /locus_tag="PLT31_01405"
     CDS             complement(17772..17990)
                     /gene="infA"
                     /locus_tag="PLT31_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPW93819.1"
                     /translation="MAKEEGIQVEGVVLEALPNAFFRVGLENGHEILAHVSGKMRRHF
                     IRILPDDKVLVEISPYDLSRGRITYRYK"
     gene            complement(17990..>18801)
                     /gene="secY"
                     /locus_tag="PLT31_01410"
     CDS             complement(17990..>18801)
                     /gene="secY"
                     /locus_tag="PLT31_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HPW93820.1"
                     /translation="YLGEQITSRGIGNGISLIIFAGIVGSLPRAILAELEMLKEGIQP
                     LAIEIFILALVIAIIGFIVFVEQANRRIPLQSPRRVVGRKVMGGQSSFLPFKVNTAGV
                     IPVIFASSIMFVPAMIASWFPNFSWAQSFAGAFMPGHITYSVIFGSLIIFFTYFYTAI
                     QYNPNDIADNLKKSGGFIPGIRPGKKTAEYIDHILTRISLPGSMFLALISVGPLHLKD
                     ALNMSFYIGGTSVLIVVGVALDTLRQLEAQLHTKNYEGFLKRGRIRGRMAS"
BASE COUNT         5725 a         3963 c         3218 g         5885 t
ORIGIN      
        1 gcgagattca cccgctcatc tattagaaat ctatttcttg agaaacattt aaaaaggaag
       61 ttctatgact atctctcttt tgaaaaaaag tgctatgttg ttgctttctg tcggagcggt
      121 attgggtgct gttgctgcgg aaggagacgc atggctttgg ccagcttatt caccaacatt
      181 aaactataat tttaaagatc aaggcaaaac ttatacaatg cctacaaaga accatgatga
      241 ttgtggttct gaagtggtag gtaaaattga tgatcgttgg tggacatttc aatggggatc
      301 tacaaagaac tctcttgtga cggatgctgc tattcgtcct atgcttgaac gatttaacga
      361 agacttcaac tatattcgtg ataccatggg ttggccaccc gatagccgtg tacgtgatgg
      421 ctacagaagt gccatttatt tgtatggttc tattccatgt tctggttcta ctaatcctga
      481 agataaaggt ggatggcaaa gttgggtagg aggatatcca gccgttgtcg cgtcttatta
      541 tcctgtatat agttttgacc ctagctgccc ttataacgat aaggtttctc agcaaggagc
      601 catgattcac gaaggaattc actctcttct cgccaccatg ggcggagcta gccatgttca
      661 ctggttccaa gaaggcggaa atacttggtt gcagcaagaa atggaagctc gtcgtggtgg
      721 gaattatact ggaatgggtt ttctaaatgc aggcccatta attgcgcctt ttgtacctat
      781 tgaatcttat tctggttggc ttttagacgg ctcttttggt ggccctggcg ctgaaggagt
      841 tgatgtacgg aatggttctc aacagctttg caattggaga aatatgttag gaggaatcca
      901 atacggaaac ttattcccta cttttcttgc tgaatgggta aatcctggag caattccttg
      961 gatttggact tatacgaaag accgttccaa atacattctt cataccatga gcgatacttt
     1021 aggcgatgct caaattcgtc gcttaattat ggaatatcgt gcaaagcttg ccatgcttga
     1081 tatgaaaaaa tggtctaatg aaatgaagaa cttattaaac caaaactttg gaacttctat
     1141 tgcatgcgaa tgggaacctt gtgccgccac tccagcatct tggaaaatga ctccttatgt
     1201 ggtcactaca gaaagcaatg gggtactcac tccagaaaat agaaccactc caggttggtc
     1261 tggcgccaac gtgattccat tgactgtttc tggaagcact gtaaaattag agcttcagcc
     1321 aattggagat aatatgagtc ttcaaatttg ttatcgtgcc acagacggta cgccagttta
     1381 tagtgaacct gttatgggaa atgctgtagc gactttaaaa ttaacgaaaa cacctcaaag
     1441 taacgttgtt tttgctgtgg tcgcaaatac agattataaa tacttaggtg atgatactcg
     1501 taaaaaacat catgattatc gccttaaatt aacagaaggc gtttctggcg ctgcagaccc
     1561 acacactaaa tggtacaata atttttctct cacttatgat tgggatgccg tttcccatgt
     1621 gggcagtagt tcttctggaa caggaagttc ttcttcgagt gccattactt cttctagtag
     1681 cgtaactctc accacaacag agatttctca taatgtggaa gttccaattt cttcaaatta
     1741 cgcttctgtg aatgtcaatt tagaaacgtc taaaatcgag caagctcttg gaattactgc
     1801 ttctcaaatt gcatcgcaat tagataacgg cgtgactttc tatggagtaa atgccgatgg
     1861 gtctctttac agtacctcca cagcaaatgc cccaggccat tggtttgatg cctctgggaa
     1921 cgtggtgtcc tattccgata acgccatcgt gttctcggaa tttgatcctc aaagtatgaa
     1981 ggtaaatatt gggcattacc cagacaaagt gactgcaggc gccaaatata cgattagtca
     2041 agcgcttatc tatggaagta aaaaagtagt tattcatttc caagtctcca ttgaatcaaa
     2101 aaccgctata gagaaatcca ttgcgtctac taatttcaat gcgcagaaat tgaatatttc
     2161 ttatgatcgc ggagtgattg tcgcaaacta ccaagtgcct gtggctggta aagtgaaagt
     2221 aagcctctat acagcttatg gagcactaat cactcatctt gtaaataaaa cgatggacgt
     2281 ggggtcttat acaaaacgaa tcgacttaaa agcgatgaac attcctcaag ggacttactt
     2341 aatcaagttt tcttatccag gaagttctga aacgaaagtc tcgattacag ctcctcgtta
     2401 aaaagcttcc agaacacaac ccaaaaaaca cgattccaac gagtcgtgtt ttttattttt
     2461 aagctatacg tcataataaa ttgtaatttt cgcgaaacgt tcagcatctc attttttacg
     2521 attttattaa acacaacatg ttcttttcga ttattttttt cgcgatacgt tctgcatctc
     2581 ttttttttgc gattttatta tacacatcat gttcttttcg atcattattt tcgcaaaacg
     2641 ttcagcatct cgttttttac gcttttatta tacacatcat gttcttttcg atcattattt
     2701 tcgcaaaacg ttcagcatct cgtttttacg attttattat cacatcatgt tcttttcaat
     2761 cattattttc gcgatacgtt cagcatctcg ttttttgcga ttttatgaaa cacaatatgt
     2821 tcttttcgat tatttttttc gcgaaacgtt cagcatctca ttttttacga ttttattaaa
     2881 cacattatgt tcatttcgaa caaatacgtc cacattttaa gcaagttttt ttatcacaaa
     2941 ccacgttgtt caaaccacgt catcttttgt gcttccattt tatttcatac aaattttcag
     3001 agagtaattt tacacttttt tcgtgtttat gcttttagcg cacaaagcaa aaattgaacc
     3061 attttgatgt aatgaagtga gtcaaccgta cttgtgattt attttaagcg ttatattgtc
     3121 ttgaaccaaa aagagcagtg cccacacgaa tcatggtagc accttcttca atagcgactt
     3181 ctaagtcgcc tgtcatcccc atcgacaatt ctgtgaaatt attaaaacga gaatctcgat
     3241 tcacaaactc ctctcgaagc ttgcgtaaaa aagaaaatcc agctctagaa tcttctgcta
     3301 aatctgtgtt tttcccaatg gtcattaagc cacgataacg caaatgagaa agcgcaggta
     3361 agctttctaa gaaagccttt gcatcactca tctgcaagcc gcttttagtg tcttcttcag
     3421 aggtattcac ctgaataagc acatctaaaa ctttgttcag ttctgcagaa tatttattta
     3481 atttttctaa cgattcaata gaatccatgg aatgaatcac gtcagcgaca agcgctgctt
     3541 tttttagctt attgctctgc acagggccaa taatatggca acggacgccc tctttttttt
     3601 gtgaaaactt ttctatggct tcttgaactc ggttttcacc aaattccctt gcccctattg
     3661 cataagcagt atcgacagct tcttgagggt gaaatttact aacccaaatc aaagtgactt
     3721 ctttttcaga tcgattacaa gaagaacaag cgagagaaat tctttgattc aaaagggtta
     3781 gcttattttt catttctgaa taatcatata ctgattccac aaaaggcccc tttatttaag
     3841 ttgatttctt tccttttttt tcttgtggaa aaagctcttc aataatttca tcgatatgct
     3901 ttttcaggtt tatttttaaa ccttttctag ctggcgcagg taactcggcc acatcttttt
     3961 cattgtcttt aggcaagaaa agcgttgtaa cgccagcatc taatgccgct aacgccttct
     4021 catttaagcc accaatcgca tgcaaagaac ctgttaagct cacttcgcca gtaaatgcta
     4081 ttttcgggtc aaccactcga ttagtaaaag cagaaagtag cgccaaagtt aaagcaatac
     4141 cagccgatgg gccatctttt ggaacagcgc cttctggtac atggatatga atatcggttt
     4201 ttcgaacaat acttggatcg acatcaaacc tttgtaaacg agcgcgcact aaactcaatg
     4261 caatttgagc ggattctttc atgacatcgc caagcttacc agtcatctgt aaatgacctt
     4321 ttccgcttaa aagcatgcat tctatcgtta gaatttctcc gccgacagat gtccaagcaa
     4381 gcccagtaat aagcccagga cgccctgggg aaggtaacct gctatccaga aaacgtggca
     4441 cccctagata ttgcgaaatt tgtttgtctg ttaatgtagg cgcactcttt ttattcaaca
     4501 ctaattcttt ggcacggtga cgcacgacac tagaaagcaa acgttctaat tcacgcacac
     4561 ccgcttctct agtatattca cgaatgattt tatgaacgat ctcttcagaa aattcaatat
     4621 cttttcctaa agaaacgcca cttcgttcac atacttctgg aatcaaatat tccgaagcaa
     4681 ttttcttttt ttcatgaata tagtaaccag gtaaacgaac tatctctaat cgatcatgaa
     4741 gcgcttctgg gatatcactt tctgtgtttg cagtcgcgat aaataaaacg cgagacaaat
     4801 caaaacctgt ttccataaaa tggtctgtaa aatcttgatt caattctggg tctaatactt
     4861 ccagcatggc acttgcagga tctccacgga aatcgctagc catcttatct atttcatcga
     4921 gtaaaatcac tgggttcatg cattttgcac ggcgtaaagc ctgaatgaat cgccctggca
     4981 tcgcgccaat ataagtacga cgatggcctc taatttcagc ctcatcacgc acgccaccaa
     5041 gagtaatgcg cacaaaactg cgttccatcg cttgggcaat agaagagact aatgttgttt
     5101 ttccaacacc tggagggccc actaaacaaa gaatgggagc tcttttttct gaaccagtga
     5161 gtttgagaac cgctaaatat tcaagaatgc gttcttttac tttttctaac ccataatgtt
     5221 tcttgtctaa ttcttttttg accttcttaa gattcaattc cgtctgacaa tattcattat
     5281 aaggtaaagc taaaaaccag tcgatgtaat tacgaatcac cgcatattct gggcttgtag
     5341 gttgcatcat tttcatgcgt tcaagctctt catcgagctt ttcttgtatt gccgcagaaa
     5401 agttttttgc tttaattttt ttggctaaaa taacatgctc agcgccacca aattcactat
     5461 cacctaattc ttcttgtaat aagcgaatct gttcattaat aatccactct ttttgcgatt
     5521 gctgcatttt ctgctgaaca tctcgacgaa cctttttcgt cacttgcaat gattctttaa
     5581 gagtgtacaa tacagattga attttctccg ctacttcctt taaggatgtg gcttctaaaa
     5641 aagcctgctt ttcagtcagc gacaaacgaa taaaaggaag tattccatag taaatatgaa
     5701 taggctgatc gagttgtctt aacgtgtcta aaaaatcttc ttggatttct tgatctaatg
     5761 cataatgaga aaactcatca atgatacttt gagctaattt cagatctttt gcagaattgc
     5821 tattgatcat ttttcgagga gccactaacg cttcaaaaaa agtttcttta gtgacttctg
     5881 taatgtcgac agtaaacaag ccttctaaca acaccttaaa atagccattt ggaactggaa
     5941 ttacattttg aatacgagca acaacaccta cagaataaag ccctgtacta gagacctctt
     6001 cttcaacaac atcgcgctgt aaagcaagaa caacaacatc atcattttta tgagcggcat
     6061 caattgcttg tatagatact tctcgcccta caagaatgcg catagcagta gagggaaaca
     6121 gaagagtatc tcttaaaggt aaaagaggta tcttttttgt aaaatcgact ataaaaggat
     6181 tattggacgt caaattattc tccatcctgt tttatgagtt tatgagaagc cgctaagcga
     6241 cttttttttt attaacagaa gaagggctgc ttttttttac agctgttttt tttgaagccg
     6301 actcaatacc atgacgaatc atttctccag tgaccgtaag atttttagtc tttgtgccag
     6361 ggagttcaaa cattgctttt tgaagagctt tttctagaac agaacgcaat ccgcgagcac
     6421 ccgttttacg agcaagcgct tcattaacaa tttcttgtaa agcatcatct gtaaacacaa
     6481 gttcaatgcc atctaattca aataattttt tgaattgttt tactaaagca tttttaggtt
     6541 gagttaaaat ttttaagaga gcttgttcat ctaactcttc caaagcaaca gccacaggca
     6601 agcggcccac taattctgga attaagccat aatgaatcaa atcatctggt tctaactgct
     6661 taaacaattc accaagtgta ttttctttat ctgttttaat atgagcacca aaacccatac
     6721 cgccttgatt cacgcgttga gcaatcactt tatcaagcgt ttcaaaagca ccgccacaaa
     6781 taaataaaat attacgtgtt ttaatatgga ctaaactttg ttctggatgc ttgcgccccc
     6841 cttttggagg gactgcagcg attgtacctt caagaatttt caacaaccct tgttgcaccc
     6901 cttctccaga tacatctcta gtaatagaag ggttcgcggt tttgcgagcg attttatcta
     6961 tttcatcaac aaaaactatt cctcgttcag cacgttccac atcataatca gccgcttgta
     7021 ataaacgcac taaaatattt tcaacatctt cgcccacata accagcttca gttaaaacgg
     7081 ttgcgtcagc aatggtgaaa ggaacatcta gaaaacgagc tagtgtttga gcgagaagcg
     7141 tctttccaga acccgttggg ccaactaaaa gcaaattgga cttttctact tcaacatcat
     7201 tttctctatt attgtcatag cgcaaacgtt tataatgatt ataaacagca acggataaag
     7261 cgatttttgc ttgatcttgc ccaatcacaa attcatctaa atgagattta atttctgatg
     7321 ggcttggtaa aggcttttga ttttgattaa tagaggtttt cttttcacga gcaaattctt
     7381 cctcaagaat attatggcac atcgcaacac attcgttaca aatatgaacg cctgcgccag
     7441 taattaattt cgtgacttgt tctgcaggct taccacagaa actacaagct acttgtggat
     7501 gattttttcc gtttttatac attacttaat aagctcatct cgccgagggt gaaaaatttc
     7561 atcgattaaa ccgaaggaaa gagcctcttc tggactcatg taaaaatcgc ggtccgtttt
     7621 ttctttcact tcttttatat cacgccctgt atgtgtggca atgatttctg aaagcgtttc
     7681 acgcgtacga acaatttctt ttgcatgaat ttgaatatcg gtagactggc ctgtagccgc
     7741 tccagaaggc tgatgaagca tgatgcgaga atgaggtaaa gcatagcgtt tcccttttgt
     7801 tcccgccgct aaaagcaccg cagccattga aatgctgttt ccaatgcaga tcgtcgcaat
     7861 gtttggacga atatgctgca tggtatcata aatagcaaga cccgcagaga cataaccacc
     7921 tgggctatta atataaatgg taatatcttt ttctggattt tcgtattcca aaaaaatcaa
     7981 ttgagccatc acattattcg ccacttcgtc attaatcgga gtacctaaaa aaatgattcg
     8041 ttcttttaat aatctcgaat aaatatcgta agcacgttct ccacgaccag tattttctat
     8101 tacggttgga atgaccacag gcagacctct ttatttagat gattcttctg cagctggacg
     8161 aatcccaact aggaaatcgg cagcggcttc aaaacgaagt tcttcacgta agctgacgat
     8221 acggccagac tgacggaaat gagcttttaa tgattcaaaa tctacttgat aagcaagagc
     8281 tagttcattt aagcgagcat cgaccatcgc ttgagtagcc tttaaatttt ccttattagc
     8341 aacgaattca agaatacggt gtttcttaat ttcacgaata gcttctggag caagtgtatt
     8401 catttgctct tcggtaggct ccacttcttc ttcagattga gaatggcgtt ttaacgtgta
     8461 tttaattagt tctacaacgc gtgcatgtgg tacttcaaaa ggatttgctt caattaattt
     8521 attcacagct tcattcactg cggttgtttt aacagcattt ctcttattgt ttgctaaact
     8581 ctcttgaatg ttttgacgca tttcatcttc gtttgcaaca ccaatttgct tgaagaattc
     8641 ttcatccata gtaggaggaa ccacttcacg aacatcagtg atttcgacct taaattgagc
     8701 tgtcttacca cggtatgctt catctttgtg atctgctgga tacacaaaat taatttcttt
     8761 tgtttcgcct tttttagcgc cctttaaacc ttcgtcaaaa cctggagaag cggattcacc
     8821 aagaagtgaa cggaattcac gttgttcagg aatttctttt gtttcgccat caataatcac
     8881 ttcaagataa ttaccaacaa cgacatcgcc ctctgcagca gcacgatcaa cggattcgtc
     8941 cttagaccac atctgtttta agcgatccat ttctgctttt acatcgtttt cagaaataac
     9001 aacgtcaggc accttgatgc ctaagtcagc atagccttga atatcaatca ctggatccac
     9061 ttcagcaacg acggtaaagg aaatatcatt ttctttatca tccttagaat cttccacttt
     9121 taagcgacct actggattga tatttgcttt tttcaattct tcagtaagaa tttgattgat
     9181 cacttcatct acagtttctt gacgaatcat ttcaccatag cgagaaagaa tcattgcttt
     9241 tggaacttgt ccttgacgaa accctttgag cgtcacttct tgacgatact tctttaattt
     9301 cttttcaaaa ggaagtttaa gttcttcttg agggatagat acttctatag tgcgcaacac
     9361 agcgctagtt tctttaatgg caacagtcat gtatactccg atataccgtg tctaaaaaca
     9421 atgcgagagg agggagtcga accctcacgc cgaaggcgcc agatcctaag tctggtgcgt
     9481 ctgccaattc cgccactctc gcgtattcga agccaaagat acaaaaaaat caaattgttt
     9541 gttattaaaa attcaattaa atcatatcaa ttatgccata aaatcgagaa ggtatgcttt
     9601 ttttaataaa tcccaatcga tttagaactc gaatgggtaa aaatgcaagc cttccaacca
     9661 aagcaaattt agtacaattt cgcattaaag caaataccat atgtggaaat aaataatcag
     9721 atacttctaa tcgatatttt tttcctatgg atttcaagat attttccaac tcgctatacg
     9781 aaaaatgcca taaatgatca gtaacagcta tatgtgagta acttaaacct aaattaaata
     9841 acgtggtaaa atcattggta caaggcaaag taaatagtac tcgttttcga gcagctcgaa
     9901 tagctttttc taaaaatgct tttggatcgt ccacatgctc taagacctca atcaaaataa
     9961 ctgtatcagc aagttcactc cctaagtctt catttggaga gacttttttt actctcacgc
    10021 cattctcttt agctgtcttt agagcctctt catctacatc aaccacaaga ggatcaaatc
    10081 catttgcctt catttctaaa gcataagaac cacaacctcc accaatatcc aaaatactag
    10141 tacctatagc ttctttcaat aaaaaatcaa caataggttg atgcgcttct ccaatctgtg
    10201 caggagggcc tagcttaaaa aaagaacttt gtccttgaag ttttcccatg agtacaatat
    10261 aaaaataact ttccatagca aaaaaaatta cttttgattt tgtgccacaa tttcttcgta
    10321 atataaaaat cggtgttttt atttcctttg taaacctctt aattcagggg atctcttttt
    10381 tcgttcagaa ttttgtagca caaaacctga gcacagcgaa ctttggatat tttggaattc
    10441 ttcaaacaga ctattccata ttttctgcta tcgccgattt cggtatgacc actttgattc
    10501 tcgctttttt tggagatcga gccaccaaag gttctctctt ccgtaatgtt ttacagttac
    10561 gcttattctc caccataatg accgctctcc tcatggtaac atttgcattt accattcgaa
    10621 gacaccaccc cgctttcaaa ggtgaactca tcctctcttt agggctactt tttcaacatg
    10681 ccttttttga ctggtatttt atttgtggaa aattttggaa acgtctattc atttcaaaat
    10741 tattacacac catctcttat gcggttgtca tgggagttgc attactttac cttaaactcg
    10801 ataaaataga gcatattgca cttgccatgc taattgctgc tttacctgcc ttcttttttg
    10861 gtgtcactca agcttttcat gcacgccttt ttagaattac aaaaagaacg ttctgcttct
    10921 tttatcttat gcttaaagcg ggtattccct atgcgattgc aagcctcgct agttttgcat
    10981 atctccccgt cgggctttac gtagctgatg tgtttgcttc tccagagttt ttaagtgcct
    11041 ataattttgc aaataaaatc attgtgttag cgtctggttt tatggttcat tttatttcat
    11101 cgagcctgat caatctccat caaaacaaag atcatttttt acatattaaa gaccagttgc
    11161 tatttacatt attcattttt acagcgtctt ccccactttg gctattccca aaagaagttc
    11221 ttcaaattct cttttttgct gccccttgga atgattctat tctagccatc agtgccgatt
    11281 gtttacgaat tctctcattt tctcttacat ttcaagcctt aagaatgggc ttaatatcct
    11341 ctcttctaaa ggataaaaaa acaaaacttt atggtctttt tgtttgtatt ggggggggct
    11401 caaacattat tttctgtaca atcgctggag cgtttttatc caatattgca atccctattt
    11461 ttgcactcag tggagacgtc gtcatcacgt tcattctctg gagttacttc tttaagaatc
    11521 gttctattcg ctggtgaaaa caatcctttc ccactactca ccgtacagac aaagaagaag
    11581 aataaaaacc atattatata cattgcttgt ttgtagcctg tagtgaagtt ccacaaaata
    11641 atccaaaaac taaaggtggt atgaaataat aagcttaatg gcgtttttgc cattggcatt
    11701 tttttataca aaaaagtaaa aataaaaccc caaaaaaatg gaactaaaaa aatgcctgga
    11761 tacccaaagt ctttataggc atcccataaa tatggaattg tatttagact actcactttc
    11821 tcgatgcttt cattaaatgg ggtatcccac ccaaaactag cttgtaagcc aggtcctata
    11881 gataataaat gagtaatacc ataaaaagca tcaatcccat aagtccaaga atgctctggc
    11941 acatcgctag ctcgattaaa agcataatct aaattccaat agttatttgc tatgtattta
    12001 taaggtaata aagccacttc gtcaactagc tcattattca ttacgtccat ttcaccgtat
    12061 tgccctttca cagaagcaac accaatgaaa aaaaacaaaa ttaatgaagc tactactacg
    12121 gccattttcc aagtaattct tttatacaaa taattagcaa gaataaagag aaatccgaca
    12181 cacatcaaat taacgcctct acttggaaac gttaaaaaac ttaacacaat cgtcaataga
    12241 caaatgattt tcgaagtatt acgaatccaa cgaattggat tataagattt gaaagaggct
    12301 acaccaaata aggctactac catagggcta gaagtgaaga aaatggcgta acttaatact
    12361 ttactagtct tgccatctaa ccataaagaa gggttatcag taagtagcac taaatttccc
    12421 gcaatcgaaa caactcccaa aaccccatat aaaaaaaatg ctaaagccaa aaaacttaat
    12481 ataaaatggc tcttccaatt atattcagta gaaagtgaaa gagtctcagg taaaacaaga
    12541 gaatagtttg aaaagcgaaa tgataatcga gctaaataac atgcacttag aaagctaaat
    12601 aatcctcctc cccaaactag ccaagtattt aattgataat cagtcattgc gggcattaac
    12661 ttaaggtaag caataccaaa cataaacgac tggactaaca catataacaa aggaggggaa
    12721 aataaatttt ttttaaaaac agaggctaaa agaagagcca ctagccctac caaaaaaaac
    12781 aaggcggatt ctggatgtgc tagccaatca aaggtcataa agtcttactt atgccaaaaa
    12841 caaatatact tcaaaaattt attccattct gttgcaaaat gtgattctct ttgcctttca
    12901 ttttcaacaa acgagagaaa gttgcctatt attaaagcaa taaaagtaaa tacaaatact
    12961 gtaaaaatca aaatcaagcc acgacgtgga gaaatcttct tatcattttc ccaaggcggt
    13021 tgaagcacat gtaagttttt aagtgtttta gcttcttcta tacgtaactg ttcactctgc
    13081 tgacgtaata ctttatataa agccacttgt accttaattt cttcttccat tctaagatat
    13141 ttagccacca aatctggagt ttttttcaaa gataataagc caacgttttc ctgctttcca
    13201 gaaagaactt tatttaatga ttgatctatt gtacgagacc gtttagtcaa ctcatcatat
    13261 tttttggagt cttcaccacg tcttagtttt tcataggagc gttcaatatt gacttcttct
    13321 tttaatattt gaatttgtcc caagtaggtt aaagtccctt caatttgtgc tgtcggttca
    13381 taaaaattat tgtctttttg gaaagcaata aattcttgtt gtaaagaatc gatatcatgt
    13441 aaacagaggg caatacgaga ttcaaaatac tctctagata atcgagcttg agtcgtttta
    13501 aaataattaa aagcagaatc tgctttttgc aaaacaaatt ctaccattct agttgctttt
    13561 ttataatcct tatctttaat cgtgatttca aacatgtttt catcattagc acttaatgaa
    13621 aaattttttc taaatgtttt tagcaaatca gcatgaaatt ttccatcaaa ctcataatga
    13681 ttgacaagat caaattgctc aatcacttga ttatgaattt cccaagagtt taaaatagtc
    13741 caaacagcat tagcatcatc atcattagat gaaaatccaa gtaaagagcc catagccccc
    13801 attcctgaac caagaaggcc acttatatta cccatggaag attgagaaga aggaggagtc
    13861 actatagcat tagaggaata tgttggttta ataacccaca ccaccaaaat aaaaacaata
    13921 attgtaggaa caacaacaat aaaggcagct aacttaaaat gttttaaggt attgttgact
    13981 actcgaagaa ataattctgt taaagaagga ttatccataa aaaccacaat acttatttag
    14041 aattggcaga attgacataa ttgatataaa cgataacagc cgtagaaacc actgacacta
    14101 tagaagctag aaacatcgtc acatccttaa atgtttcata acggcttttt ggaatttcaa
    14161 tataatcccc tggcaatatc tgatctgttg aaacattcac ctttattttt tcctgattcc
    14221 cacgaacaac acttacttga gtccaagtcc ctgtttgagt taatactcca gaagcagcaa
    14281 tataatcaat tgcatgccat gaggattcat aaggaatttt tcccatagcc attacagctc
    14341 caccaacata aacaaaaagt gttttcacag aaaattctat ttccgtttga ggaggcactt
    14401 tccaatgatt taattctttt gcagaaataa cttttgtcgt tttatctgga taacgaatcg
    14461 tcacagattg atatcccata ttatgcatac ggtccccacc tgctttctca aaatactgag
    14521 ctatagtaga ggaactatca taagggacgc ctacacgact gccaccaggg aatattagag
    14581 caatcatatc taaagtattc ataaaaggca cataaattcg atcaccctgt tcaagaagaa
    14641 tatcttgctc aaaatttcct ttgtcaaaaa tttcaacaca attgatatga agggtatccc
    14701 ctttacgaat cacccataca tcatcttttc ggcctgttcc taaaatatta tctgcctttt
    14761 gcaacgccat acttaaacga gtttgagggt caacaaccat ttgcccgact tgtttaacag
    14821 cacccatgac acttaccaca aacttcttca tttgtgaaag ctgtacaaac acatattcag
    14881 gattatattt tttggaagcc acttttacaa tttcttctcg tgcttctgca agtgttttgc
    14941 ctgcgatctg cactattcca cactcttcaa tcgcaataga tccatcagat aaaatttgaa
    15001 gagaaaaaaa atcttgttca agcatgatat ctaagtaatc accagggccc aatttatagg
    15061 aagaatcaat cacaccttcc acagccattg gctccatcgc tattgaatta cgagcagatt
    15121 tcgttgaagg taaagagaac ccaaaagatg atggactttt tatgtccgat gctgaaaaag
    15181 caagacacga aaagaaaagt aaaattaaac ttattctatg aaaacacata tatgaacctt
    15241 acgaaaagca agcaaataaa aacttaagat acaaaaaaac caactttaaa agttggcttt
    15301 tttgtattag aatctaaaaa gactcattct gcagtttttg ctgcaccttc aaccatgtca
    15361 attggcttta cttcggcttt tttctgagaa ggctttactg ctttttcaac aatttcatct
    15421 tctacaagtc cgattagagc catttctgct gcatcaccag gacggttagg gccgagtttt
    15481 aacactcgag tataaccacc atttcttgat gcataacgtg ggccaaggac atcaaaaagg
    15541 ccttgaacta cttttgggtc gcgtacaaag cgagccgctt cacgacgagc agaaaggtcg
    15601 cctttttttg cataggttat taaacgatca acaacacttt tcgctaactt tgccttatgc
    15661 aaagtggtgc gaatataacg gtttccttgt tcggtctcca agcctttttc taaaatagaa
    15721 gtagcgaggc tacgaagaat ggcacnnnnn nnnnnacgct tatgttgtgc gtttacaccc
    15781 aatttcttgt tctttacacc gtgtctcatt tctattaatc cttcagataa tcatcgacgt
    15841 tcatcccaaa actgagaccc atcgaagtta acacctcgtt aagttccacc aaggacttcc
    15901 taccgaagtt cttgtattta agcatatcat tttctttatt acgtacaagt tcagcaatcg
    15961 tatgaatgtt agccattctt aagcagttgc ttgagcgaac ggataattct aattcatcca
    16021 cgcgcatacg aagaagagtt gaaatgcgtt gacgttcctc gtcttgttcc atttcttcag
    16081 gagattcgag atcaccttcg aaattaatga atgtttccaa atgatcgaca agtaactttg
    16141 ctgcataagc aagtgcatct tcaggatcaa tagaaccatc agtagtgact tcaagttcaa
    16201 gacgattaaa atctgtacgt tgtccgacac gagtatcgct gatatgcatc gccaccttca
    16261 atactggatt gaaattagcg tctattgcaa tttcgccaat aggagcattt ggatctttca
    16321 attcatcagc agtcacataa ccgcgaccag atgaaatttt caattccatg cttaaagaag
    16381 cgttcccatt caacgtcgca atatgtacat caggcgttaa aatcactaca ttcggatttt
    16441 cttcaatatc agcagctgta atcttgccgt caccggacat atccagccgt aatgtttcat
    16501 cgtgatcaga aagcagtttt actcgaatac ccttgagatt caagatgatg tcagtaacat
    16561 cttcttttac tccaggaatc gaagtaaact ctttatcgac accgtcgatt ttcacagaga
    16621 caattgccgc gccttgcaaa gaagacaaca acgtacgacg aagagcatta cctaaagtaa
    16681 taccccaacc gcgttcaaga gcttctacga caaacttggc gtagcgccca tcttcgccag
    16741 tttccacttt ctgaaaactg cgcggcatct gaagggactt ccacatcatt agcgatacct
    16801 cattaaatta aattcttctc ttctttttag gacgacatcc gttatgcgga acacccgtca
    16861 cgtcacgaat agacagaact tcgaggcctg catttttgag ggctcgaaca gcagattcac
    16921 gaccgccacc agcacctttt actcggacat ctactttacg catgccaagt tcaaaagcct
    16981 tgcgaccagc tgcttcagag gcaagctgag ctgcaaaagg tgtactctta cgtgaaccct
    17041 taaaaccagc gtttcctggg gaaccccaag caacaacgtt tccacgagca tctgtaatag
    17101 atacaatcgt attgttaaaa gaagcattta cacaagcaat accttgaatg tcaacacgct
    17161 tctttccttt cttaactttt acttcttcag aagcaactgt ttcagttgca gctgtaactt
    17221 cttttaattc ttcatcagcc acgaactttc tccttacttc ttcttgttag ccacggtttt
    17281 cttcgggcct tttcgtgtac gtgcattggt acgtgagcgt tgaccgcgaa caggcaagcc
    17341 cttgcgatga cgcaagccac gataacatcc aatatcttgt aaacgtttaa tgttaagagt
    17401 agtttctgcg cggagttgac cttcaacaga atactcttct tcaagaagat ggcggatttt
    17461 accttgctct tcttcagtta aatcatcaca ttttttgtct ttgtctatac caagttgagt
    17521 acaaacttta cgagcggtaa ataaaccgac accaaaaatg gcggtcaaac cgtactcgac
    17581 agttttagtt ctcggtaaat cgacaccagc gatacgtgcc atgagatctc ctatccctgt
    17641 ttctgcttgt ggcgggggtt tttcgaacag ataatacgca aaacaccttt acgacggata
    17701 attttgcagt tttcacatct tggtttgatg gaggctttga ttttcatagg ttaccttttt
    17761 ttttttacct attacttgta acggtaagtg attcgtcccc tagataaatc ataaggggat
    17821 atttcaacta gaactttatc atcaggcaaa attcttataa aatgcctgcg catttttcct
    17881 gaaacatggg ctaatatttc gtgaccattt tctagaccaa cacgaaagaa cgcgtttggt
    17941 aatgcttcaa gcacgacgcc ttctacttgt atgccttctt ctttagccac taggatgcca
    18001 tcctgccgcg tatgcggcca cgttttaaga aaccttcata atttttggta tgcagttggg
    18061 cttcgagttg tcgaagagta tccaacgcca caccgaccac gatcaagacc gaggtgccac
    18121 caatatagaa agacatattc aaagcgtctt ttaaatgtag tggaccaacg ctaatcaagg
    18181 ccaagaacat agatcctggt agtgaaatgc gagtcaaaat gtgatctata tactctgcag
    18241 tttttttacc tgggcgaata cctggaataa aaccacccga tttctttaga ttatcagcaa
    18301 tatcatttgg gttgtattga attgctgtat agaaataggt gaagaatata atcaaggatc
    18361 caaatattac gctatacgta atatgtcctg gcataaaagc accagcaaag gactgagccc
    18421 aactaaagtt tgggaaccat gatgcaatca tggctggcac aaacataatg gaagaagcaa
    18481 aaatcacagg gatcacacca gctgtattca ccttgaaagg caagaagcta gactgaccac
    18541 ccattacttt acgtccaacc acacggcggg gactctggag tggaatgcga cggttcgctt
    18601 gttcaacaaa aacgatgaat ccgataatag caatcactag cgctaaaatg aaaatttcaa
    18661 tagctagagg ttgtatacct tctttcaaca tttctagttc agcaaggata gctcttggaa
    18721 ggctacctac gataccggcg aagataataa gagaaatacc attaccaata ccacgcgaag
    18781 taatttgttc gccaaggtac a
//
LOCUS       DAOOBB010000014        18428 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_4004_length_18428_cov_4.787786, whole genome shotgun sequence.
ACCESSION   DAOOBB010000014 DAOOBB010000000
VERSION     DAOOBB010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 18428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18428
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4004_length_18428_cov_4.787786"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PLT31_01415"
     CDS             <1..492
                     /locus_tag="PLT31_01415"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HPW93821.1"
                     /translation="VTSHRETICNILSGALNQKVNYNDIPLNDLGRPDLSSWGIDANW
                     THSHNTCVLVYSFNANVGVDLEFHKKRPIKLANRFFHSDEIQAIHDQKDPLLSEHLFF
                     QLWTRKEAFYKCFGGSFFKDVLPRSMLHEDEVMFSFLEPSLGDLEERPYSFCIVASLK
                     HNS"
     gene            complement(510..929)
                     /locus_tag="PLT31_01420"
     CDS             complement(510..929)
                     /locus_tag="PLT31_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93822.1"
                     /translation="MIVEIAALCDAATDQGGRLNILGAFDRLITPLPFIYPQCSAVFR
                     IRYQRSEATMHSFRLSISNLQDQNLIPPLESEIPFPPLGDFFESGTINMILNMQRLHI
                     AEEGKYMLRLRVDQIEVALLPLFVKDSTPHEPNPLAN"
     gene            complement(926..6163)
                     /locus_tag="PLT31_01425"
     CDS             complement(926..6163)
                     /locus_tag="PLT31_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93823.1"
                     /translation="MRLRVIFVISLLFLLLFGCSREDSIRENMTLKNNHSIIFVFQDT
                     LENMSSFRDSISNLDSVWVPDTISITVNDTLYFMGFLRHNANKVFRYAWQMQPNDSAR
                     LSSNGKILTYVFDTTGIFSPLFIAVDGNGARDTTGKNQFIRVIDTPPNLFLPTDTIWN
                     RAAQKGSFIIRANDSLGQVTRISLDWNGDKKADTSYVPIFSKETKDSLFINLPFDSAY
                     LNSDFNQNIRISVYDEDGNTTTDSLVIHYNQLPVVELLQPTNNGRYSKDARFAFYYKA
                     EDNDISNQLRYHIRVGKSPTGDGTPPILTDANLIAGPIKEKSFEAIDENGKWFMDTKL
                     IGRLYWQVYVTDGFDTTVSPTQTFFLGNVMETTGTFRGTVLLEGKTVHNGIKIEFLDS
                     LGNRAYTNTNAKGIFTIDLPPGIYHMTARDTLGYGFSPELVKNKFIEAGDLIDLGSIT
                     LKDPSAPVISVDKLADTLNYRDFTITGRFADYGSQVELARGFLDDNAITISTVKLNSW
                     SMELKDMPDGPHTFKMIALDSAGLISDTLMLNFFIQASSMSLLVNGNSAAMVNQKDEL
                     VFTASVDNLNPPSDSIVWTWVVNGDSYTQTTLIEDNKSTLTIKAEDIANEEDGVFYEM
                     EATTQNQTAKAKVRFGFIGNDPIVYFSSPKTGTTVSINDTVRFNVIGQAGAFATSYSL
                     SWLCDAALTSSYGCPTNTTTNNTLLAWSNIGMKKVIIKIDDDNSGIAYDTMKVNVISD
                     PPAINIKSSEDTLVKKINATVPIEVTASDKYGTISSLQWGCSNGNVAFDNTIDYSATP
                     LESISETISILLPGTESSAYRCIVKAFDDDLETASDTIYFRVILDKPTVTLHTKLSTE
                     KINAVVPFKAIASDSLGFIEHYYVACNNSLNSLSFKEISAPDTTISMPSNATTWYCVI
                     RVVDNDQLSAEDTARYTVVLDPPTVIASEDSLTMTINDEILLDAMANDAMGHIALYEW
                     SCGPAGTAGKTFGWSSTTTPRYTAVMPSTPQNNYLCVIRVTDDDEQTAMDTTHITVLL
                     DAPKVTVANDNLLLREGLNIALNASASDLMGSITKREWSCGIASEINDHWKTVSQYDT
                     VWKAPSTAQVNYLCVARATDDDGNLATDTTRILFSTENPIVTVTKEKIYVKAGDYVDL
                     DATINDVWNDIEWFNWICATPTSDSLKNPFESWRYDGDFAHAMGPDHTSPGKDLLCVV
                     FVSETGTHYLAKDTTQVFILSEPPIGVISAPDTTFIWSGDETVTPNGKYFYSANIHAS
                     NSTIGTLGNANLEDFWWQFSHYDPTYWYQGETDGSIDTSIAEFNDAFIRRQSEGSITL
                     RLDYRDSTTTETDPNYKAAFLYRHRAEQVNKTIRFRKAWNNESYGEDSVIVLTNYPVA
                     PHLIVHNDKPTIAFVNESGKIEVQKFNGTSQWNSIGSPITPSGTPSFIQLAVNADADL
                     FVAYLDSTGHRGHILKSNGGTSPWVELGSGTGNSHIAKMKMKIRPSTKAPVLAWIGNT
                     DPSSSTLTKSYYQAWNTADVAFDSPISIFSEALRELDIAFDGSSNWLAMGVQTNTDYQ
                     LLNRYYNNRNTYRSTPGITSTSAESIQLQFANNKFYLAFRNRNNGDKTGFPQIYSATN
                     NAGSGVNWSTNGNIHSGFGRMSSNVSMVLNASGNPVAVFDESNRATNSQVHAYRFDGA
                     SWNVLGENELPYFKNLFVMSKKYYLRGHQPSIAITSNGDVFIAMRALEQPASASTVLR
                     NKNNGPLVMKYLGDNWENP"
     gene            6218..7051
                     /locus_tag="PLT31_01430"
     CDS             6218..7051
                     /locus_tag="PLT31_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HPW93824.1"
                     /translation="MFIVPLWYAVSLFFVLGACVGSFYNVVIYRMPRGLSLIKPASFC
                     PTCNKHIPIYHNLPILAWLFLRGKSACCGNRISIQYPLVELLCALLGALSVILAEWPL
                     SFEAPWTQITNSFALFWLLLAIVPVCAVDFKHHLIPDSISIGGVVVGLLLSLIPGGIS
                     PVESLVGVLIAGVGLYGLGLLMTFLLKKEAMGFGDVKLLAGYGAFMGWFYAFEVLIFA
                     AFLGLLVMLPLKFFKKIKGSEAENTSFGEIPFGPFLSIMAPIVYLWGDKILKVYLSFV
                     Y"
     gene            7071..8519
                     /locus_tag="PLT31_01435"
     CDS             7071..8519
                     /locus_tag="PLT31_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HPW93825.1"
                     /translation="MYEDVKINLNPNDMPKEWYNLAADLPTPMLPPLGPDGNPISPEQ
                     MTAIFPPNIVEQEMSTKSWISIPEEIRQHLMRWRPTPLRRAYWLEQYLKTPAKIYYKD
                     ESASPAGSHKGNTAIAQVWYNKQAGTKTITTETGAGQWGGALSFACKLMGIDCKVFMV
                     KVSFEQKPFRKALMRTWGGTCIASPSEVTEVGRKVLREYPDCPGSLGIAISEAVELAV
                     GDKTGSTKYALGSVLNHVMLHQTVIGLEAKKQLEMIGIKKPDIVLSCAGGGSNFAGMA
                     FPFTYDKINGQDIEIIPVEPTSCPSLTRGVFAYDYGDLSGFTPLLPMHTLGHDYIPSS
                     IHAGGLRYHGMSPIISQAVNEGLYNPTSVGQNECFLAAVRWARTEGWVIAPESSHTVA
                     AVIREAIKAREEGKEKTILFTMSGHGYLDMVGYEKFLNKELQDFSLPQSEIDETEARL
                     KSKGFPEAPIRRTGKWTPGNIDHSQYADHGKI"
     gene            complement(8659..9522)
                     /locus_tag="PLT31_01440"
     CDS             complement(8659..9522)
                     /locus_tag="PLT31_01440"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPW93826.1"
                     /translation="MKAILVGHGAMGTKHFNRLSALGLSFEAVLDSTEDLNRFTASLP
                     SLSKSDTLVVIASPASTHASHASFFLKQGFSVFIEKPIATSAIEARELLSIKKPSQAL
                     FVGHSERYSPGFQLFQKEFQKKISSIETLRFTRCHPYSPRGRDVNVALDLLVHDLDCF
                     FSLFPKENQKSFEIIDSNLSADQDEASLKIKQPIHSNHALIIDFYVNRNAPFSERKIE
                     LFDNQNNIIKTFSFLDAQDPIKDTLFYEYEALFNAIQMPDSSIEKERLESACLAVETA
                     LKIKTNPSEFL"
     gene            complement(9528..10052)
                     /locus_tag="PLT31_01445"
     CDS             complement(9528..10052)
                     /locus_tag="PLT31_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93827.1"
                     /translation="MKKILLMIILFFLSVNSFADEYDDYASSEAQKNGTSKKAGSPLV
                     GIGVNILTGSNYEFRTSILLEQNITLYLISQFVRSGDDDDNSNYNFSLGAGASFSLPS
                     SLLPTYAGATFKYIHPGENTNGAAINAFAGVKALFLNAFEIAGELGVYFEYIHDAGTN
                     ISLRPAVYLSWYFI"
     gene            complement(10094..12667)
                     /locus_tag="PLT31_01450"
     CDS             complement(10094..12667)
                     /locus_tag="PLT31_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HPW93828.1"
                     /translation="MTILEKLGLALPSVENPARYMGGEANSITKNHDEMLAKMAFVFP
                     DLYEIGMSNNGMRILYHVINKEPDLLCEVAFAPWFDMAEAMKRLDIPLYSHASWTAVS
                     DFDVIGITLQTELNFTNVPYILELSRISTWTEERDEKEPFIIGGGPAMANPEPVADFF
                     DAFIIGDGERSIIEVMRTIGEGRQAKKSRQEILKNLSTLDGVYVPSLRPVIFNKYNQA
                     IPAEPATGSYSSTKGVRRLFIPEMNPADYPTKNLIGNMQLVHNRFSIEVMRGCTQGCR
                     FCQAGIWYRPCRELHPDDVIDLAKQGIRATGERELGLLSLSTADYKPVEALADSIIDD
                     PFFGNIDVSLPSIRVNSFGQSLAGKAAALKGGRSTTFAPETGSTRIRKMINKTISNED
                     MYNAAEYAFSNKFNKIKLYTMIGFPTENLDDMEEFCQLIERLVQIGRAHSKGIQIAVS
                     IGIFIPKAFTPLQWSPFVDKETALSHIRFVRERFYHHPNVKINWSAWETSVLEAFYSR
                     GDRSISKLVYQAYKKGMIFESDNNTINYEAWEQLWQESKYDYSWLFETRDLDEVFPWD
                     FIHAGVSKSYLKNEWIKAFDENSVSVPNCKWGDCQKCGIPGAGSDTKLASLPVKYAAP
                     SRTPEEIKTLLESRKTAQGTGYLYKITFEKTGLSRFLPHQNTISCFERTFFCMDIPIK
                     FSEGFSPKPKIVNIGALPLGLETYCEVISIELLQKLDLSDSNRSALIEKLNAFLPHGI
                     KIIGIEPLQEKLSKNFPKAMIFKYTPEKVPSTLETLFKEKKLPIVFNHRGVEVDLNEH
                     ILDLQIINNAIITKVKCNDQGGSVSPYTIYAGLMQLENDASRLDEVARTFLIQKTAME
                     W"
     gene            12764..14065
                     /locus_tag="PLT31_01455"
     CDS             12764..14065
                     /locus_tag="PLT31_01455"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPW93829.1"
                     /translation="MNWIFYFIWVIAFILGVIVAYFVPESTLPINAKFIFVGFWGAAL
                     GAVLVTILSKKETNLSDPFNFPKPETELIPIVPRVEAPPVIESPIQQKKTVNTVIDSL
                     IFPTEAWNHFCRKVLKNRPFYEAIDSLASCLPQMFQNASGILYMYSGEQTELHKIIRF
                     GKNVIGDDVITPAECASFHIGDIVVTDFSNELLSPGCTHLHHRPKGFSFCAPLEAAEE
                     HFGILTLQVDEIPQGETPEFWKVKLSVVTATLGLYVANQNLNLKFQEHSIRDTLTGVF
                     NRRYMEESLQREVASANRHRTPIGVIMIYPDQVKSILEARGRHAVEQLLWELGQRIPK
                     YIRTEDIPCRYEGETFCIILPGADLYISQDRAEKIRREIEGLEISYGNILLSTTLSIG
                     IATMPQNANTAQGLIGDSLTALHTAIKDGKNRVVVAPMEYR"
     gene            complement(14131..16068)
                     /gene="thrS"
                     /locus_tag="PLT31_01460"
     CDS             complement(14131..16068)
                     /gene="thrS"
                     /locus_tag="PLT31_01460"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPW93830.1"
                     /translation="MNQIELTLPDGSVRSVASGTTGLEIAKSIAEGLARKALAIKLND
                     KILDLNRPLKESGTFKIITPSNEDADALFVLRHSCAHVLAEAVCDLFPGTQLAYGPAI
                     DKGFYYDLMTPTPLVFDDLAKIEKRMKEIIKEDRPFIRLELNKEEGLARVQKDKYKLD
                     NAERALQREDSDGTLSFYVTGKQNENWEDLCAGPHIPNTGKIKAFKLLSLAGAYWHGD
                     QASDQLTRIYGTCFADKEGLETYLHFLEEAEKRDHRKIGKELDLYHIEDHSPGMVFWH
                     PKGTIMVNALKQFIREKLNRLGYDEVITPEIVDKSLWIKSGHADKYNENMFKTMANDR
                     EMAIKPMNCPCHIEIFNQGLKSWRDLPLRMAEFGKCHRYEPAGTMHGLMRVRGFVQDD
                     AHIFCTEEQIASEVSDFCKLVKDIYAEFGFNDILVKFSTRPEKRVGSDDLWDKAEQAL
                     EDATKLANLDYQLNPGEGAFYGPKLEFVLKDCLGRDWQCGTIQVDFNLPQRLGAEYVG
                     KDNQKHIPVMLHRAAVGSIERFLGILIEEFMGDFPLWLAPVQARVLPISEKFVDYAKQ
                     VERDLVNAGVRVEIDESNEKLGYKIRQCELQKIPFLLVVGEKEVSEKKISVRQRKEGD
                     KGSMSVSEFIEMTKKARDVIR"
     gene            16347..17075
                     /gene="ispD"
                     /locus_tag="PLT31_01465"
     CDS             16347..17075
                     /gene="ispD"
                     /locus_tag="PLT31_01465"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HPW93831.1"
                     /translation="MNTINPNKASFAVVLPAGGLGKRMGSSIPKQLLSLRGKPVYFHS
                     LETFLSMDEIAEVILVVPKDWKSHFEQELLDGNALSKEYQEKLKIVEGGKERWESVKQ
                     GVLAISKSSHVLVHDVARPFISKDIIRESCLALETKGACIVARPVNDTVKVVQDGVIQ
                     NTLDRTTVWLAQTPQSCSVELLKSLYKKIEQNPLDFVPTDESSILEYFNIPVYVIRGD
                     ALNDKITTPEDWERFSSNSFYKNQ"
     gene            complement(17060..17725)
                     /locus_tag="PLT31_01470"
     CDS             complement(17060..17725)
                     /locus_tag="PLT31_01470"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPW93832.1"
                     /translation="MKKLSKLVLLIACIASSAFSIENLSIGGRASGNFNLIWGENSRD
                     GAYGFGFNVGGSVLYEINPMIQINPELTFAFRKHFFSHEINVILGSSSSDFAVTFLNL
                     DIPILARLQVIQPVFFEIGPLLSFNLSASYFSEMKSSSSLTFTQEEDIDESSVFEFGL
                     VFGVGKKLDIGTGLDVNFRFILGLTNTYEDNLNNIFGVELPSIDRDNKTMMFQLGATY
                     WFL"
     gene            17904..18284
                     /locus_tag="PLT31_01475"
     CDS             17904..18284
                     /locus_tag="PLT31_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HPW93833.1"
                     /translation="MNSIKLTKSLEDYLEMVLILRKTLGKVRVKDLASALSVKMPSVA
                     KAVNLLKQQKLVNQESYGDIELTTLGETTAKKILSRHLLLKNFLMNIGVTEEVANEEA
                     CLMEHILSPETLDCIETFNKKFEK"
     gene            18325..>18428
                     /locus_tag="PLT31_01480"
     CDS             18325..>18428
                     /locus_tag="PLT31_01480"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA domain-containing protein"
                     /protein_id="HPW93834.1"
                     /translation="MKPGDQAEIIGYQGGDQRYKSKLLAMGLVRGVVL"
BASE COUNT         5577 a         3298 c         3663 g         5890 t
ORIGIN      
        1 gtaacgtctc atcgagaaac gatatgtaat atcctatcgg gtgcattaaa tcaaaaagtg
       61 aactataatg atattccctt aaatgattta ggacgtccag atttatcttc ttggggaata
      121 gatgctaatt ggactcattc gcataatact tgtgttctag tttattcttt taatgcaaac
      181 gttggggttg atttagaatt tcacaaaaaa agaccgatca aattagctaa tcgttttttt
      241 cattcagacg aaattcaagc gattcatgat cagaaagatc cgcttctttc agagcatctg
      301 ttttttcagt tatggactag aaaagaagct ttctataaat gctttggcgg tagctttttt
      361 aaggatgtat tgcctcgttc tatgctccat gaagatgaag taatgttctc ttttcttgag
      421 ccttctttgg gagatctaga agaacggcca tattcttttt gcatcgtcgc ttctttaaaa
      481 cacaattcgt agaattaaaa agaacgtttt taattagcta gagggtttgg ttcgtgaggg
      541 gtcgagtctt ttacaaataa aggtaataga gctacttcaa tttgatccac gcgtaagcga
      601 agcatatatt tcccttcttc agcaatatga agccgctgca tatttaaaat catgttaatc
      661 gtgccgcttt caaagaaatc gccaagagga ggaaatggaa tttctgattc tagaggagga
      721 attaaatttt gatcctgcaa attagaaata cttaatctaa aggaatgcat tgtcgcctca
      781 gaacgttgat aacgaattct gaaaacagca ctacactgag gatatataaa aggtaatggc
      841 gtaattaggc gatcaaatgc tccgagaata ttaagcctgc caccttgatc ggtggcggcg
      901 tcacaaagag ctgcaatttc gactatcatg gattctccca gttgtcacct aagtatttca
      961 tcactagggg accattgttt ttatttcgta gtactgtact tgccgatgca ggctgctcta
     1021 aggctcgcat ggcaataaag acatctccat tgctagtaat agcaatactt ggttgatgtc
     1081 ctcttaaata atatttttta gacataacaa ataggttttt gaagtaagga agctcatttt
     1141 ctccaagaac attccatgat gctccatcga agcgataggc gtgaacttga gaatttgtag
     1201 ctctattgct ttcgtcaaaa acagccactg gattgccgct tgcattaaga accatactta
     1261 cattggatga cattcttcca aaaccagaat gaatatttcc gttagtgctc cagttgacac
     1321 cagaacccgc attattggtg gctgaataaa tttgtgggaa tccagtttta tcgccattat
     1381 tgcggtttct aaaggctaag tagaatttat tgtttgcaaa ttggagctga atcgattcag
     1441 cgcttgtcga ggtaatccca ggagttgatc tatatgtgtt tctgttattg taataacgat
     1501 tcagtagttg ataatctgta tttgtttgga ctcccatagc tagccaattt gaagaaccgt
     1561 caaaagcaat gtctaattct ctgagagctt cgctaaagat gcttattgga gaatcaaacg
     1621 ccacatcagc tgtattccaa gcttgataat aggatttagt taaagtcgat gacgaaggat
     1681 ctgtatttcc tatccaagct aatacgggtg cttttgtgga aggacgaatt ttcatcttca
     1741 ttttagcgat atgagaattt cctgtaccag aacctaattc aacccaagga gatgttcctc
     1801 cattagattt taatatatgc ccacgatgac ctgtgctgtc taagtaggca acaaataaat
     1861 ccgcatccgc atttacagcg agttgaataa aagaaggtgt accactagga gtaatgggtg
     1921 aaccaatact attccactgt gaagttccat taaacttttg aacttctatt ttgcctgatt
     1981 cattcacgaa agcaatcgtt ggtttatcat tatgaacaat taaatgtggt gcgacaggat
     2041 agtttgttaa aacaattaca gaatcttctc cataagactc attgttccaa gcttttctaa
     2101 aacgaatggt tttattcact tgctcagcac gatgtctata aaggaatgct gccttgtaat
     2161 tagggtctgt ttctgtggta gtactatctc tataatccaa acgtaatgta atagaacctt
     2221 cagattgtct gcgaataaaa gcatcattga attcagcaat gctagtatca atcgatccgt
     2281 cggtttctcc ttgataccag tatgttggat cataatggct gaattgccac cagaaatctt
     2341 ctaagttcgc gttaccaaga gttccaatag tggaatttga agcgtgaata tttgcagaat
     2401 agaaatattt accatttgga gtcaccgttt catctccact ccaaataaag gttgtgtcag
     2461 gggctgaaat cacaccaata ggtggctcac tgagaataaa cacttgggtt gtatctttag
     2521 caaggtaatg agtccctgtt tctgaaacaa atacaacgca gagaaggtct tttcctgggg
     2581 atgtatgatc aggtcccatg gcatgagcaa aatcaccgtc atacctccat gattcaaaag
     2641 gattttttaa agaatcacta gtaggagttg cgcaaatcca gttaaaccat tcaatgtcgt
     2701 tccatacatc attaatagtt gcgtctaagt caacgtaatc tcctgctttc acataaattt
     2761 tttctttagt cacagtgact ataggatttt ctgtagaaaa taaaattcgt gttgtatctg
     2821 tggcaagatt tccgtcatcg tctgtagcac gagcgacaca gagataatta acttgagcag
     2881 tcgaaggggc tttccatacc gtatcgtatt gggatacggt tttccaatga tcatttattt
     2941 cagaagcaat accacaagac cattctcttt ttgtaataga tcccatgagg tcagaagccg
     3001 atgcatttaa tgcaatattt aagccttcac gtaaaagaag gttgtcattt gcgacagtga
     3061 ctttaggcgc atccagtaat actgtaatgt gcgttgtatc catagcggtt tgttcatcat
     3121 catctgttac gcgaataaca cataggtaat tattttgagg ggtagatggc atgactgcag
     3181 tgtagcgagg cgttgttgta gaagaccatc caaaagtctt gcccgcagta cctgcaggtc
     3241 cacaactcca ctcgtataga gcaatgtggc ccatcgcatc attagccatg gcgtctaaaa
     3301 gtatttcgtc atttattgtc atggttaaag aatcttcact tgcaatcacg gtgggtgggt
     3361 caagaacaac tgtataccga gctgtatctt ctgctgaaag ctgatcgtta tcgacaactc
     3421 gaataacaca gtaccaagtg gttgcattcg atggcataga aattgttgtg tcaggggcag
     3481 atatttcttt gaatgataag ctattaaggc tattgttaca tgctacgtag tagtgttcga
     3541 tgaaccctaa tgaatcagag gctattgcct taaaaggaac aacagcatta attttttctg
     3601 tggaaagctt ggtatgtaat gtcactgttg gcttatctaa aatgactcgg aaataaatag
     3661 tatcagaagc agtttctaga tcatcatcaa aggcttttac aatacagcga taggcgctgc
     3721 tttctgtgcc tggtaataga attgaaatgg tttccgaaat agactctaaa ggtgttgcag
     3781 aataatcaat ggtattatca aaagcgacgt tcccattact acaaccccat tgaagagaac
     3841 taatggttcc atatttatct gatgccgtta cctcaatcgg gacggtggca ttaattttct
     3901 ttacaagagt gtcttcggac gatttaatat taattgcagg tggatcagag atgacgttga
     3961 ctttcatggt gtcataagct ataccagaat tatcgtcatc aatttttatg atgacttttt
     4021 tcatcccaat gtttgaccaa gcgagaagag tattgttagt agtagtattt gtaggacagc
     4081 cgtagctaga agttaatgct gcgtcgcata gccatgaaag gctataagag gtcgcgaatg
     4141 cacctgcttg cccaattaca ttaaagcgga cggtgtcgtt tatggataca gtggttcctg
     4201 tttttggaga ggagaagtaa acaataggat cgtttcctat aaaaccaaaa cgtacttttg
     4261 ccttagctgt ttgattttga gttgttgctt ccatttcata aaatacacca tcttcttcat
     4321 ttgctatgtc ctcagcttta atagtaagcg tcgatttgtt gtcttcaatc aaagtggttt
     4381 gagtatagga atcgccattg acaacccaag tccaaacaat ggaatcgcta ggtggattaa
     4441 gattatctac agaagcagta aagacaagct cgtctttttg attgaccatg gctgcactat
     4501 ttccgttgac gagtaaactc atcgaactgg cttgaataaa aaagttaagc attaaggtgt
     4561 ctgaaattaa accagcacta tctagtgcga tcatcttgaa tgtgtgaggt ccatcgggca
     4621 tgtctttaag ttccatagac catgagttta atttaactgt agagatcgta atagcattat
     4681 catcaaggaa gcctctcgcc agctctactt gagaaccata atctgcaaaa cgaccagtaa
     4741 tggtaaagtc tcgataattt aatgtatcgg ctaatttgtc tactgaaata acaggagcac
     4801 ttgggtcttt taatgtaata gatcctaagt caattaaatc tcctgcttca ataaatttgt
     4861 ttttgactag ttctggagag aagccataac ctaaggtatc tcttgcagtc atatgataaa
     4921 ttccaggagg aagatctatg gtgaatatgc cttttgcatt cgtgtttgta taggcgcggt
     4981 ttccaagaga atctaaaaat tcaattttga ttccattatg aacggttttt ccttctagta
     5041 atacggtccc tcggaaggta cccgttgttt ccatcacatt tccaagaaag aaggtttgag
     5101 taggggaaac agttgtatca aatccatccg tgacatagac ttgccaatag agacgtccaa
     5161 ttaatttggt gtccataaac catttgccgt tttcatcaat ggcctcaaag cttttttctt
     5221 taatcgggcc agcgattaag ttagcatctg ttagaatagg aggagtacca tctcctgtag
     5281 ggcttttccc cacacgaata tgatatctta attgattgga tatatcgtta tcttctgctt
     5341 tataatagaa tgcaaatcgc gcgtctttgc tataacggcc attatttgtg ggctgcaata
     5401 gttctacgac tggtagttga ttgtaatgaa tcactaaact gtcagtggtg gtattaccgt
     5461 cttcatcgta aacactgatg cgaatatttt gattaaaatc agaattgagg tatgcggaat
     5521 caaaaggaag atttataaat aaagaatctt ttgtttcttt tgaaaaaata ggaacgtagg
     5581 atgtatcggc ctttttatct ccattccaat ctagagaaat acgtgtgact tgacctaagg
     5641 aatcatttgc tctaatgata aaagaacctt tttgggcggc ccgattccaa attgtatcgg
     5701 taggcaagaa tagatttgga ggggtatcaa tcacgcgaat aaattgattt tttcctgtcg
     5761 tatctcttgc tccatttcca tccactgcaa taaataaagg agaaaaaatg ccagtagtat
     5821 caaaaacgta cgttagaatt ttgccgttag agctaagcct agctgaatca tttggttgca
     5881 tttgccaagc ataacggaat actttattcg cgttatggcg taaaaagccc ataaagtaaa
     5941 gagtatcatt tactgtaatg gatatagtgt caggaaccca aacggagtca agattactaa
     6001 tagagtctct aaaggatgac atattctcaa gagtatcttg aaaaacaaaa ataatcgaat
     6061 gattattttt taaagtcata ttttcacgaa tcgagtcttc tctggaacat ccaaagagca
     6121 ataaaaacaa caaagatatc acgaaaataa cacgtaatcg cataaacaaa tccttttctc
     6181 atcaactatc gtttaaaagt aaatttatct tttaaatatg tttatagttc ctctttggta
     6241 tgcagtatca cttttttttg ttcttggtgc ttgtgtaggt agtttttata atgtggtcat
     6301 ttatagaatg ccacgagggc tttccctaat aaaaccagct tcattctgcc cgacttgcaa
     6361 caaacacatc cctatttatc ataatctgcc tatcctagca tggctttttt tacgaggaaa
     6421 aagcgcttgt tgcggtaata gaattagtat tcaatatcct ctagtggagc tattatgtgc
     6481 tcttttagga gctttgagtg ttattttagc ggaatggcct ttgtccttcg aggctccatg
     6541 gacgcaaatc actaattcat ttgctttatt ctggctatta cttgctatag tgcctgtttg
     6601 tgccgtagat ttcaagcatc acttgattcc tgactccatt tctataggtg gtgttgtagt
     6661 tggtttattg ctttctttaa tacctggagg gatttctcca gtagaaagtc ttgttggtgt
     6721 tcttattgct ggagttgggc tttatgggtt gggtcttttg atgacatttc ttctaaaaaa
     6781 agaagctatg ggttttggtg atgtaaagct acttgctggt tatggtgcct ttatggggtg
     6841 gttttatgct tttgaagttc tgatatttgc cgccttttta ggccttttgg tgatgctacc
     6901 tttgaagttt tttaagaaaa taaagggctc tgaagcagaa aatacctctt ttggcgagat
     6961 tccctttggt ccttttcttt ccatcatggc tccaatagtt tatctttggg gtgacaaaat
     7021 tttgaaagtg tatctttcat tcgtttatta attcacttag ttaggttctt atgtacgaag
     7081 acgttaaaat caatctaaat ccaaatgata tgccaaaaga atggtataat cttgccgctg
     7141 atttacctac tccgatgcta cctcctcttg gtcctgatgg gaatcctatt tcgcctgagc
     7201 aaatgacggc tattttccct ccaaatattg tagaacaaga gatgagtaca aagtcctgga
     7261 tctctattcc tgaagagatt cgacaacact taatgcgctg gcgtccaaca cccttgcgtc
     7321 gtgcttattg gttggaacaa tatttaaaaa cgccagcaaa gatttattat aaagatgaaa
     7381 gtgcaagtcc tgctggtagc cataaaggca ataccgccat tgcgcaagtt tggtataaca
     7441 agcaagcagg aacaaaaacg attaccacag aaactggtgc tggtcaatgg ggtggtgctt
     7501 tatcttttgc ttgtaaactg atgggtatag actgtaaagt ctttatggtg aaagtgagct
     7561 ttgagcagaa gccttttcgt aaagctctca tgcgtacttg gggtggaact tgtattgcta
     7621 gtccaagtga agtaacagaa gtcggtcgca aggttctgcg tgagtatcct gattgcccag
     7681 ggtctttagg aatcgctatt tctgaagcgg ttgaacttgc cgttggtgat aaaacgggaa
     7741 gcactaaata tgctttaggt tccgttttga atcatgtgat gttacatcaa accgtgattg
     7801 gattagaagc aaaaaaacaa ttagaaatga taggtattaa aaagcctgat attgttttat
     7861 cttgtgctgg tggaggctcc aattttgcag ggatggcttt cccttttacc tatgataaaa
     7921 tcaatggtca agacattgaa attattcctg tagaacctac cagttgccct tctttaacac
     7981 gtggtgtatt tgcttacgat tatggagatc tatctggatt tactccatta ctacctatgc
     8041 atactttagg ccatgattac atcccaagta gcatccatgc aggtgggctt cgctatcatg
     8101 gtatgtctcc aattattagc caagccgtaa atgaagggtt gtataatcca acctctgttg
     8161 gtcagaatga gtgcttctta gcagctgttc gttgggctcg taccgaaggc tgggtgattg
     8221 ctcctgaatc ctctcacact gtagcggctg ttattcgtga agcaataaaa gctcgtgaag
     8281 aaggcaaaga aaaaacgatt ctatttacga tgtctggaca tggttatctt gatatggtcg
     8341 gctatgaaaa gttcttaaat aaagaacttc aagattttag cttacctcaa agtgaaattg
     8401 atgaaaccga agctcgctta aagtcaaaag gcttcccaga agcacctatc cgcagaacgg
     8461 gaaaatggac tcctggaaat attgatcata gtcaatacgc ggatcatgga aaaatctaat
     8521 ttcttagata aattaaaaac ggaatcttaa tcaaggttcc gtttttttat tgtcatttcg
     8581 ccctattctt aataacgaga taattcgttt tgcttgttgg caaaagggtt ccagagagta
     8641 tcgtttgatt attgataatt aaagaaattc agaaggattg gttttgattt ttaacgcggt
     8701 ttcgacggca aggcaggcac tttctagtcg ctctttttct attgaagaat caggcatttg
     8761 aatggcatta aataatgctt cgtattcata aaataaagtg tctttaattg ggtcttgagc
     8821 atctaaaaaa gaaaacgttt ttatgatgtt attttgattg tcaaagagtt ctatttttcg
     8881 ttctgaaaaa ggggcgttgc gattcacata aaaatcgata ataagagcat gattactatg
     8941 aatcggttgc tttattttta gtgaggcctc atcttgatca gctgaaagat ttgaatcaat
     9001 aatttcaaaa gacttttgat tctcttttgg aaagagagaa aaaaaacagt ctagatcatg
     9061 gactaaaaga tctagagcca cgtttacatc tcgacctcgt ggagaataag ggtgacagcg
     9121 agtaaagcga agcgtctcta tagaagatat ttttttctga aattcttttt gaaataattg
     9181 aaacccagga gaataacgct cgctgtggcc tacaaataaa gcttgtgatg gctttttaat
     9241 acttaaaagt tccctagcct ctatggcgct tgtggcaata ggcttttcaa taaagacaga
     9301 aaagccttgt tttaagaaaa aagaagcgtg agaagcgtgg gtagatgcag gacttgcaat
     9361 gactaccaac gtatccgatt ttgatagtga tggtagagag gcagtgaaac gattcaggtc
     9421 ttcggtcgaa tctaaaacag cttcaaagga aagccctaat gcggacaatc gattgaaatg
     9481 tttagttccc atggctccat gcccgaccag aatcgctttc atagaaatta aataaagtac
     9541 caagaaagat aaacagctgg tcttaggcta atgttagtac ctgcatcatg aatatactca
     9601 aaatagacac ctaattctcc agcaatctca aaggcattta ggaacaacgc ttttacgcca
     9661 gcaaaggcat taatggctgc tccattagta ttttcgcctg ggtgaatata tttaaaggta
     9721 gcgcctgcat atgtaggtaa taaactggaa ggtaatgaga aagatgctcc tgctcccaga
     9781 gaaaaattat agttggagtt atcatcatca tctccagatc gtacaaattg agaaatcaaa
     9841 tacagagtga tattttgttc taaaagaata gaagttctaa attcatagtt ggatccagtt
     9901 aaaatattaa ctccaattcc aactagaggt gaaccagctt tttttgatgt tccatttttt
     9961 tgtgcttcgg aagaagcgta gtcatcatat tcgtcggcaa acgaattgac tgatagaaaa
    10021 aagagtatga tcatcaatag tatttttttc atagaggaat cctttgtttt aggttttgtt
    10081 gttaagatgt tttttaccat tccatcgctg ttttttgaat taagaaagta cgagccactt
    10141 catctaaacg agaagcatcg ttttccagtt gcattaggcc agcgtatatg gtataaggac
    10201 ttacgcttcc gccttgatca ttacatttca cctttgtaat aatggcatta ttgataattt
    10261 gcagatctaa aatatgttcg tttaaatcca cttctacacc acgatggtta aatacaatgg
    10321 gtaacttttt ttctttgaat aaagtttcta gagtagaggg aactttttct ggagtatatt
    10381 taaatatcat ggctttcgga aaattcttag aaagcttttc ttgtagcggc tcaatcccaa
    10441 taatttttat gccatgaggt aaaaaagcat ttagcttttc aattagtgca gaacgattgg
    10501 aatcagataa gtctagtttt tgtaaaagtt caatacttat gacttcgcag taggtttcaa
    10561 gccctaaagg caaggcccca atgtttacga ttttaggctt aggactaaat ccttcactga
    10621 atttgattgg aatatccatg cagaaaaaag ttctttcaaa gcaactaatt gtattttgat
    10681 gaggaagaaa acgactgagc ccagtttttt caaaagtgat tttatagaga taacctgttc
    10741 cttgagctgt ttttcggctt tctaaaagag tttttatttc ttctggagtt cggcttgggg
    10801 cagcgtattt tacggggaga gaagctagtt tagtatctga gcctgctcct ggaataccgc
    10861 atttttgaca atcgccccat ttacagtttg gaacgctaac agagttttca tcaaaagctt
    10921 ttatccattc gttttttaaa taggatttag aaacgcccgc gtgaatgaaa tcccatggaa
    10981 atacttcatc taaatcgcga gtttcaaata accaagaata atcatacttt gattcttgcc
    11041 agagttgttc ccatgcttca taattgattg tattgttgtc gctttcaaaa atcatgcctt
    11101 ttttatacgc ttgatagaca agtttcgata tagaacgatc tcctcgacta tagaaagcct
    11161 ctagtacaga agtttcccag gcactccaat tgattttcac atttggatga tgataaaaac
    11221 gttctctaac aaagcgaata tgagaaagtg ctgtttcttt atctacaaaa ggcgaccatt
    11281 gcaaaggtgt aaatgctttt ggaataaaaa taccaatgct aacggcaatt tggatccctt
    11341 tagagtgagc tcgtccaatt tgaactaagc gttctatgag ttgacagaat tcttccatat
    11401 cgtcaagatt ttctgtcgga aaaccaatca ttgtgtacaa tttaatcttg ttaaatttat
    11461 ttgaaaaagc gtattccgcc gcattgtaca tgtcttcatt actgatcgtt ttattgatca
    11521 tttttctaat acgagtagaa cctgtttctg gagcaaaggt ggtgctccgc cctcctttca
    11581 aggcggctgc tttaccagcg agactttgtc caaaactatt gactcgaatg cttggaagag
    11641 aaacatcaat atttccaaag aatggatcgt cgataataga atcggctagg gcttctacag
    11701 gcttgtaatc ggctgttgat aaactgagta aaccaagttc tctttctcca gtagcacgta
    11761 tcccttgttt tgcaaggtct atcacatcat caggatgtaa ttctcgacaa gggcgatacc
    11821 atattcctgc ttggcaaaaa cggcatcctt gtgtacaacc acgcatgact tcgatgctaa
    11881 aacgattgtg tacaagctgc atgttcccga ttaaattctt agtgggataa tcggcggggt
    11941 tcatttctgg aataaataaa cggcgaaccc cttttgtaga agaataagat cctgtcgcgg
    12001 gttccgctgg aatggcttga ttatatttat tgaaaatgac tgggcgaaga gagggaacat
    12061 aaacgccatc tagagtggat aaatttttaa ggatttcttg acgcgatttt ttcgcttgac
    12121 gaccctctcc aatggttcgc ataacttcaa taatagagcg ttctccatca ccaataataa
    12181 acgcatcaaa gaaatcagca acgggttctg gattagccat cgcaggtccg ccaccaatga
    12241 taaacggttc tttttcgtct ctttcttctg tccatgtgga aatacgactt aattctaaaa
    12301 tataggggac attagtgaaa ttaagttctg tttgtaaggt aattccaatg acatcaaaat
    12361 cggaaacagc tgtccatgag gcatgagagt aaagaggaat atctaagcgt ttcatcgctt
    12421 cggccatatc aaaccaagga gcgaaagcga cttcacaaag aaggtctggt tctttgttta
    12481 ttacatggta taaaattcgc atgccattat tggacatacc aatttcgtat aggtcaggaa
    12541 aaacaaatgc cattttggca agcatttcat catgattttt tgtaatactg ttggcttctc
    12601 cacccatata tcgagctgga ttttcaacgg acggaagagc taggcctaat ttttcaagaa
    12661 ttgtcatata gaaataaaga tagaaatgaa aaaaaagctt ttttatgaat taaatgttat
    12721 aatatctctt tttatcctgt ttaaaaaaag taaattaatt accatgaatt ggatatttta
    12781 ttttatttgg gtcattgctt ttattttggg agttattgtt gcttattttg tacctgaatc
    12841 tactcttcca ataaatgcta aatttatttt tgttggcttt tggggggccg ctcttggagc
    12901 tgttctcgtc actatccttt caaaaaaaga aacaaatctt tcagatcctt ttaattttcc
    12961 aaaaccagaa acggaattaa ttcccattgt acctagagta gaagcccctc cagtgattga
    13021 atcccctata caacaaaaaa aaacagttaa cactgttata gactcattga tatttcctac
    13081 agaagcctgg aatcattttt gccgaaaagt attgaaaaat cgcccttttt atgaagcgat
    13141 tgatagtttg gctagttgtt tacctcagat gtttcaaaat gcttctggta ttttgtacat
    13201 gtatagcggt gaacaaacgg agcttcataa gattattcgc tttggaaaaa atgtgattgg
    13261 agatgatgtg attactcctg cggagtgtgc tagttttcat ataggtgata ttgtggtcac
    13321 cgatttttct aatgaattat tatctccagg ttgtacgcat ttacatcatc gtcctaaagg
    13381 tttttctttt tgtgctccat tagaggccgc tgaagaacat tttggtattt taactcttca
    13441 agtggatgaa atacctcaag gagagactcc agaattttgg aaagtaaaat tgagcgtggt
    13501 gactgctaca ttaggtcttt acgtcgcgaa tcaaaatttg aacttgaaat ttcaagagca
    13561 tagtattcgt gatactttga cgggtgtttt taatcgcagg tacatggaag aatctctaca
    13621 acgagaagtg gcttcagcca atagacatcg cactccaatt ggtgtgatta tgatttaccc
    13681 agatcaagtg aaatccattt tagaagctcg tggtcgccat gctgtagaac agttgttatg
    13741 ggagttaggg caacgtattc caaagtatat tagaacagaa gatattccat gccgttacga
    13801 gggcgaaacg ttctgtatta ttttacctgg tgcagatctc tatattagtc aagatcgagc
    13861 tgaaaaaatc agacgagaaa tagaaggcct tgaaatttct tatggtaata ttctcctttc
    13921 aaccacatta agtattggta tagcgacgat gcctcaaaat gcaaatacag ctcagggctt
    13981 aatcggagat agtttgaccg ctcttcatac tgcgattaaa gatggtaaaa atagagtcgt
    14041 tgttgcccct atggaatatc gttaaaaaac taattataaa aaagccccac attcatagag
    14101 aagtggggct tgatgtttta aatgcttgta ttagcgaatg acgtctcggg ctttctttgt
    14161 catttctata aattcagaaa cactcatgga acctttatcg ccttcttttc tttgacgaac
    14221 agagattttc ttttcagaaa cttctttttc gccaacgact aaaaggaatg gaattttttg
    14281 aagttcacat tgtctgattt tatagccaag cttttcattg ctttcgtcaa tttcgacgcg
    14341 gacaccagcg tttacaagat cgcgttctac ttgttttgca tagtccacga acttctcaga
    14401 aataggaagc actcgtgctt gcacgggagc taaccataaa gggaagtctc ccataaattc
    14461 ttcgatcaga ataccaagga aacgttcaat ggaaccaacg gcagcacgat gtaacattac
    14521 tgggatatgc ttttggttgt ctttaccgac atattcggcc cctaaacgtt gtggcaaatt
    14581 aaagtcgact tgaattgtac cacattgcca gtcacgacca agacaatctt ttaatacaaa
    14641 ttcaagctta ggaccataaa aagcaccttc tccaggatta agctgataat ctaaatttgc
    14701 taacttagtt gcatcttcta atgcttgttc cgccttatcc caaagatcgt ctgaaccaac
    14761 acgtttttct ggacgagtag agaatttgac tagaatatca ttaaacccaa attcagcata
    14821 aatatctttc actagtttgc agaaatcgga gacttcggaa gcaatttgtt cttcagtgca
    14881 gaaaatatga gcatcatctt gaacgaatcc gcgaacgcgc ataagaccat gcatagtgcc
    14941 tgcaggttca taacggtggc attttccaaa ttcagccatt cttaaaggaa ggtctctcca
    15001 agattttaag ccttgattaa aaatttcaat atgacaagga cagttcattg gcttaatcgc
    15061 catctcacga tcattagcca tggtcttaaa catattttcg ttgtatttat cggcgtgccc
    15121 agatttaatc caaagagact tatccacgat ttctggagta atcacctcat cgtatccgag
    15181 acggtttaat ttttcgcgaa taaattgctt tagggcattg accataatgg ttcccttagg
    15241 atgccaaaat accatgccag gagagtggtc ttcaatatgg tataaatcga gttctttacc
    15301 gattttgcgg tgatctcgtt tttctgcttc ttcaagaaaa tggagataag tctcaagacc
    15361 ttctttatca gcaaagcagg tgccatagat tcgggttaat tgatcgcttg cttgatctcc
    15421 atgccagtaa gcgcctgcaa gagaaagaag cttaaacgct ttgatttttc cagtattagg
    15481 aatatgtggg ccagcacaaa gatcttccca gttttcgttt tgttttccag taacgtagaa
    15541 acttaaagtc ccgtcgctat cttcgcgctg gagagctctt tctgcgttat ctaatttgta
    15601 tttgtctttt tgaacgcgag caaggccttc ttctttattt agctctagac gaataaaagg
    15661 tctgtcttct ttgatgattt ctttcatgcg cttttcgatt ttagctaagt catcaaaaac
    15721 gagaggggta ggagtcatca aatcgtagta aaagcctttg tcaattgcag ggccataagc
    15781 taattgagtt cctggaaaaa ggtcgcatac ggcttcggca agcacatgag cacaagagtg
    15841 cctgagaaca aataaagcat ctgcatcttc attacttgga gtaataattt taaatgtgcc
    15901 gctttcttta agaggacggt ttaagtctaa aatcttgtca tttaatttga tggcaagagc
    15961 ttttctagct aaaccttcag cgatgctttt tgcgatttct aggccagtgg ttcctgatgc
    16021 tacggaacgt acggagccat cagggagagt gagttcgatt tgattcatga tattatatcc
    16081 tttatttccc tggaaatacg acatccagcg gtaaaggcga atttaggaat aaaaacgttt
    16141 tttatcattt gtttttttaa gaaaacaccc tagggcttat tgatttttta agaaaaatca
    16201 ttgaatgagt ttattagtaa cctgaaaaat gatgattttg ttgctttgaa ggcttcaaaa
    16261 agagggagca aaaccacagc ttgtattttc tgatggaatc cattttaaac gttctttttc
    16321 tattaaatgt tgttaatttt tgaactatga acacaataaa tccaaataaa gcttcgtttg
    16381 ccgtggtctt acccgcaggt ggtttaggca aacgcatggg atcttctatt cctaaacaat
    16441 tactttcctt gcgtggaaaa cctgtttatt ttcacagtct agagactttt ttatcgatgg
    16501 atgaaatagc tgaagtgatt cttgtggttc ctaaagattg gaagagtcat tttgaacaag
    16561 aacttttaga tggaaatgcg ctttcaaaag aatatcagga aaaattaaaa attgtcgaag
    16621 gtggaaaaga acgttgggaa tcagttaaac aaggcgtttt agctatttca aaatcctctc
    16681 atgtgttagt gcatgatgtg gcaaggcctt ttatttctaa agacattatt cgtgagtctt
    16741 gtcttgcgtt agaaactaaa ggggcttgta ttgttgctcg cccagtgaat gataccgtaa
    16801 aagtggttca agatggtgta attcaaaata ctttagatcg cactacggta tggcttgctc
    16861 aaactcctca aagttgttct gtagagttat taaaatcact atataaaaaa atagaacaaa
    16921 atcctttaga ctttgttcca acagatgaat cctcaatatt agaatatttt aatatacctg
    16981 tttatgtaat tagaggggat gcattaaatg ataaaataac cactcccgaa gattgggagc
    17041 ggtttagtag caattctttt tataagaacc aataagtagc acctagctga aacatcattg
    17101 ttttattgtc tctatcaatg cttggaagtt ctactccaaa tatattgtta agattatctt
    17161 catatgtatt ggttaggcca agaataaaac gaaagttaac gtctaaacca gttcctatat
    17221 ctagcttttt acctacacca aataccagtc caaattcaaa tacactcgat tcatcaatgt
    17281 cttcttcttg tgtaaaagtt aaagaactag atgatttcat ctcagaaaaa taagaggcag
    17341 ataaattaaa ggataacagt ggccctattt caaagaatac gggttgaatc acttgtaagc
    17401 gagctagaat tggaatatca agattcaaaa aagtaacggc aaagtcagat gaagaagaac
    17461 ctaggatgac attaatttca tgagaaaaga agtgcttacg aaaggcaaaa gtgagttctg
    17521 gattaatttg gatcatggga ttgatttcat aaagaacaga acctcccacg ttaaagccaa
    17581 atccgtaagc gccatcgcgg gaattttctc cccaaataag attaaaatta ccactcgctc
    17641 gaccgccgat cgaaaggttt tctattgaaa aagcggaact tgctatacaa gcgatgagta
    17701 agactaattt ggatagtttt ttcatgtttc tctccgaaat gaaatagtta acctaaatat
    17761 atttctttta tctaaaaatt atcatactaa tatagggtgc ttttgaatga gtattacatt
    17821 cttgcgtaat ttaagaaaaa atgaagcttg tttttacaat aatttattac tagattagcc
    17881 aaagctaaca aatggaaggc gttatgaact caataaaact cacgaaaagt ttagaagatt
    17941 atttagaaat ggttcttata cttcgtaaaa ccttagggaa agtccgtgtg aaggacttag
    18001 cttcagctct ttcagtgaaa atgccttctg tggcaaaagc ggttaatcta ttaaaacagc
    18061 agaaattggt aaaccaagag tcttatggtg acattgagtt gactacgctc ggagaaacta
    18121 cggcgaaaaa aattcttagt cgtcatttat tgctcaaaaa ttttttaatg aatataggcg
    18181 ttactgagga agtggcaaat gaagaggcct gtttgatgga acatatttta tcgccagaaa
    18241 ctttggattg tattgaaact tttaataaga agtttgaaaa atagtatgaa ggaagtagag
    18301 aataacataa tcacacttgc tgacatgaag ccaggagatc aagctgaaat catcggctat
    18361 cagggcggtg atcagcgtta taaatcaaaa ctactcgcta tgggtcttgt tcgaggagtt
    18421 gttttaga
//
LOCUS       DAOOBB010000015        18402 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_4011_length_18402_cov_4.529569, whole genome shotgun sequence.
ACCESSION   DAOOBB010000015 DAOOBB010000000
VERSION     DAOOBB010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 18402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18402
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4011_length_18402_cov_4.529569"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="PLT31_01485"
     CDS             <1..948
                     /locus_tag="PLT31_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPW93835.1"
                     /translation="IAKMHGTKQMTLPCPMCNVINGGAHSSAPIDFQEFMVAPVGAKT
                     FSKGLQMVTEIFHALKKVLKDGGYDTTVGDEGGFAPGVALKKDPKSKFGYTIKGVMTL
                     ENALKALGTATENAGYKFGKDIKIALDVASSEFCDKKGANETYTFKKSTNKTLTSKQM
                     VSYYEKLCSKFPIFSIEDGLDEADWAGWKVLTDKLGAKVNLVGDDLFVTNPTIFNEGI
                     EAGIANAILIKVNQVGSVSETLAAIKRAQDEGYAPVVSHRSGETEDTFIADLAVGTGA
                     GQIKTGSLSRTDRVCKYNRLLRIEEELGKDAVYAGDPRK"
     gene            1117..2979
                     /locus_tag="PLT31_01490"
     CDS             1117..2979
                     /locus_tag="PLT31_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPW93836.1"
                     /translation="MVDLKTIVRAAFTIPALCAFFVGCGGQQDSSSEGSLPRQETLYL
                     SGQQWGSPATFNPLAESWMAAWPVGGRFNLMYEPLITYNTLNGNFEPLLGTLVDSLSN
                     NDSIVVDLNPAAKWSDGKPVTSKDVKFIFELGFNYPGAVTSFVTEQITGINVDTVYSV
                     DSASMKTTMLNERLSFIVAKNKRNNPLSVKDALQAIRIVPEHVFSKLLAENKNSLDEI
                     KKMMMDKDPVVSGPYNLQSYSPTKIILKRRDDYWGNAALHDGKLPAPKYVVHPIYKSN
                     EHSTIALREGSLDASMSFVPRIWHKKISGVKTWYNDPPYFVPGAMPMLFINTTKAPLD
                     NKIFRRAIATAIDYVAIRMFAVSNYTSEIISGLIMPTSLEKEFINKEDCDKYGIDFSI
                     AGDEKLKAVQTMIQQAGFKSVFEKDGSLSHMENAKGEKMPTMFITSPAGWTDWEAIVT
                     IAVEGMRKAGIDVREGFVDGGQYWPAMGTGNFDLIMHKPVADVSPSLPWSRFEAVMSS
                     RDWRPIGDWAGTNIGRYNAPGSKEFRPEIDSLLALIPLMTDKEQIKDAYHQLNRIFME
                     DQPAIPLTYLPEQFYEFSDQVWTNWPTEKNPYAPPQLPWIASGTKILWNLQLSK"
     gene            2989..4353
                     /locus_tag="PLT31_01495"
     CDS             2989..4353
                     /locus_tag="PLT31_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW93837.1"
                     /translation="MLKQFPTLRYVLQKGFWYLLTFVVAVAINFALPRLGENNPVDII
                     MGKAAQGLSPDEAKKKKESLLISFGMAELNDDGSVKYEPVIGPNGQPLTEKKPVLDES
                     GNLIIKMVPALDASGKPIVEKKPVLDSAGNPMTEMVPVLDKNGKPVKKKGKVVEEAVP
                     VMQDVTIETPDTLKQDVVVLQKDPKLASGLSQFFVYIKNVFRGDFGLSYSQYPKPVID
                     IIKESLPWTIAIQLPTIILGWIIGNLLGAFAAYKRGIFDKVFFPLAMFLNGVPFFVFG
                     MLLVALFSITLGWFPAMGAYGPEKVPSFSLDFLKSLGYYYVLPFFSVFPILLSGQATG
                     MRSMSIYELGTDYMKYAKWLGLREGKIIGYIFRNAMLPQLTGLAQSLGTMVGGALITE
                     MIFSYPGLGMAMLTAIQQNDYATIQGCTLMISTCVLLANFGVDVLIAVFDPRVKAGLQ
                     MGDK"
     gene            4356..5201
                     /locus_tag="PLT31_01500"
     CDS             4356..5201
                     /locus_tag="PLT31_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW93838.1"
                     /translation="MVKLLKNLLKSPMFVIGVSIFLITLAIAFLGPVFYDVDTNARDI
                     VAGPYAPSSAEHILGTDHLGRDYMSLLISGLRSSLYVGFVAGIIATTLGVFIGLFGGF
                     RGGWVDEVLNMGTNLFIVIPSFVILVLISSAVKDGRSLTLIGLIIGLTTWSWSARAVR
                     AQASSLRSRDHIALARINGASSFTIVIKHVLPYLLSYVFMVFIMQISSGILSEASISM
                     IGLGPLDSTSLGIILNQAKDNGALSDGIWIAFLPATLIITLTVFALYLVNTSMEGVFN
                     PRLRK"
     gene            5219..6205
                     /locus_tag="PLT31_01505"
     CDS             5219..6205
                     /locus_tag="PLT31_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW93839.1"
                     /translation="MSENIFEVENLSLHYLGRFGDKTHAVTDVSFNMKQGEILGIAGE
                     SGCGKSTLVSGLMGMCIPPLYPTSGDVRVRGESLMNRTVEDIRSNVLAQKVSMIPQGA
                     FNALNPVRKIKDIAADVIAAHQKPGSSMKASEIYERLRSRFDLFGMDTDRVLNSFPIQ
                     LTAGERQRSVIGISTLLNPQMVIADEPTSALDVSTQKEVIKMIFDLLDKGIFSTMIFI
                     THELPLLYHVADNIAIMYAGEIVEKGTAEQVVKDPRHPYTKALMGAMLSSEKSQRTRK
                     PVAIEGAPPSLAKKIVGCRFAERCSFARPECKKNTQNIRMVGDREVRCEYAQ"
     gene            6195..7016
                     /locus_tag="PLT31_01510"
     CDS             6195..7016
                     /locus_tag="PLT31_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HPW93840.1"
                     /translation="MLSNKSIVFSAKNISKTFGAGKKITTAVNNVSFDIYDGEIISIV
                     GGSGCGKSVLAKIMLGLYGPSKGKFHYNGKKITNLKKHWNDVQSVFQDPFSCFNQFFT
                     IRSQLEDAFNILKKKPSKEEIKRRVDEGLLAVNVTPSDIEGKYSFELSGGQMQRMLLA
                     RIFALRPKVLIADEPTSMVDACVRANILDYLMKLKEELQMTIVFITHDIGLANYVSDR
                     IFIMHDGMIVNSGTPEEVLDNTRETHTLKLLDDIPEVHKKEWIKNSHRSKNKEAS"
     gene            complement(7135..7788)
                     /gene="rpe"
                     /locus_tag="PLT31_01515"
     CDS             complement(7135..7788)
                     /gene="rpe"
                     /locus_tag="PLT31_01515"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HPW93841.1"
                     /translation="MSIPQIIAPSVLNANFLELGKSLSAIEKGGAGLIHLDIMDGHFV
                     PNISFGPGIADNIKKGTSVPLDCHLMITNPDNYVEAFAKAGAHYICVHAETTFHLDRL
                     LHRIIELGCKPAVSINPATPIESILNVLDIVDMVLIMSVNPGFGGQGLIPYTLDKIKR
                     LRDLKPEMNIQIDGGIKLDNIQLAQEAGANIFVVGSAIFNTPDPEATCRQFVKKIQK"
     gene            7861..8259
                     /locus_tag="PLT31_01520"
     CDS             7861..8259
                     /locus_tag="PLT31_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HPW93842.1"
                     /translation="MAISKKTTAATPAKKAACGAKKTVDTKKVKATVKKETKVETKVS
                     AKAAKTVKAAPAKKEASKDVVFSIDAPLATTVAVAGCFNNWSVTKNKLKKDKKGVWSV
                     KIPLAVGRYEYKFVCDGVNWDEGENKVKNV"
     gene            8252..9511
                     /gene="lysA"
                     /locus_tag="PLT31_01525"
     CDS             8252..9511
                     /gene="lysA"
                     /locus_tag="PLT31_01525"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HPW93843.1"
                     /translation="MSDHFIPESVFQRGAESFGTPFWLYDRETIEARVREVKVFDVVR
                     YAQKACSNLSILSLMRKLGVVVDAVSAGEIVRALRAGYQGGVGKQTSQIVYTADIFDA
                     DALALVKEHHLAVNIGSPDMIQQLADYGVRTELMLRVNPGFGHGHSRKTNTGGDLSKH
                     GVWHEQIKDCIKLAQKNGMWITGLHMHIGSGSDFGHLAQVCDAMFDAARLLGGHLKTI
                     SAGGGLPVSYHENEKNDRIDINAYYKLWDNTRKRIEQSVGHSVSLEVEPGRYLIAESG
                     FLVSEIRAVKKQGTNTFYLVNAGFTDLVRPSFYGSYHEISVIAKDHRPLSERQQVIVG
                     GPLCESGDVFTQEEGGFVVSRELPVAQVGDLLVLHDVGAYGSAMSSNYNSRLLAAEIL
                     YSKGSLEVIRERQSFEQIMQNDRIVDL"
     gene            9694..10293
                     /locus_tag="PLT31_01530"
     CDS             9694..10293
                     /locus_tag="PLT31_01530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HPW93844.1"
                     /translation="MLRVSLLFSLILLFVLPTYAVKPSNSYKDPRDKQKYRTIKVGDR
                     IWFADNLNYKMDGSFCYKEDESNCQAYGRLYTWDAAIKACPVGYHLPTHGDFESLWQA
                     AGADFNAAYLLKAEYGWSGETNGNDTLKFSAMPAGNRFDDETYGNLSKFAFFWSSEAS
                     TEKQNARVWYMTSKSMAFSFMNKPKIYGFSVRCVQSSKK"
     gene            10437..11603
                     /locus_tag="PLT31_01535"
     CDS             10437..11603
                     /locus_tag="PLT31_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HPW93845.1"
                     /translation="MSDRFVVTGTFTDDPFAIDVAQYIGLREDISDTVALKTFANSEF
                     CPRYVLDADDIEHIGRRLHGKIVLICTVSTHDRSRNDLAMRNCILARAAKDNGAERVV
                     LVEPDLFYSAQDRGPHRFGELEADREISDLKKFDGQAFTSLLYAQLLKTSGVDAVVTV
                     HNHSVKVQKLFNDVFEGHFHNLIPTEVYAHYIKTSNFVQTGKDGDNLVIVAPDKGATP
                     FMNNMWDALELPLCKRVVMNKVRSGEREVSMTLSDHSEVGMDYLEGKDVIVFDDMVRT
                     GTTIVQCCEYLKTGHPNRVCFGVTHFHTSPEAREKLNSQFIDEILTTGTLPDIMNRDC
                     QGRLRRKLTVIKLGKWMARHILQMYGLDDGRFEKNFYKIDMSSKNPRWPPPHLY"
     gene            11640..12971
                     /gene="nhaA"
                     /locus_tag="PLT31_01540"
     CDS             11640..12971
                     /gene="nhaA"
                     /locus_tag="PLT31_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HPW93846.1"
                     /translation="MAKKNSEVTDDLFPKGRIDRVIAPIYRYLQVETTSGVILIIATA
                     LALFWANFAGNSYHALWEMPLGISIGRFDFTRSLHWWINDGLMTLFFFVVGLEVKGEI
                     INGELKNPKTASLPIIAALGGMIIPAVIYLMTCPPGTTAGWGVPMATDIAFVVGCMAI
                     LGNRIPKGLHIMILTLAIADDIGAIIVIALGYSGTIDGHYLLMALGLLALTHVFFRIG
                     IRNLIVHVLLGAVIWFAFVNSGIHPTLAGVALGLLTPAIPWVKKGAVNSYVHKVNDFL
                     AGGPSPENVTQYDIFRTLQKCSCESVAMQERLLHVLHPWVSYVIMPLFALANAGVIIR
                     GDSFSGVTLSIGLGLVLGKPIGIFLFSFAAVKMRIAKLPAGVSLKMLLGGGALAGIGF
                     TMSLFVSELAFVNSRSLLDGAKIGILFGSFLSALIGMLIIYAHKKSPLNKE"
     gene            complement(13009..13587)
                     /locus_tag="PLT31_01545"
     CDS             complement(13009..13587)
                     /locus_tag="PLT31_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HPW93847.1"
                     /translation="MSKSIKGTETEKNLLKAFAGEAQARNRYTYFSGVAKKEGLVQIA
                     DIFEETANQEKEHAKRFWKFLEGGNVEITATYPAGVSSNTLDNLKAAAEGENEEWSDL
                     YPAFAKIAREEGFPEIAKAFEMISIAEKQHEKRYRDLYNNLMEGKVFKRNEKVVWRCR
                     NCGFLHEGTEAPELCPACLHPQAHFELLGENW"
     gene            13802..14485
                     /locus_tag="PLT31_01550"
     CDS             13802..14485
                     /locus_tag="PLT31_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HPW93848.1"
                     /translation="MLDLLAVQSSTAHPTVITMVYTLLLAFVLSSLIGYTYEKTFLGL
                     SYSRNFVQALVLSSVVAATVMQAIGDNVGRGLGMLGALSIVRFRTSFKDPRDIMFLFA
                     ALGAGIGCGVFAWGVAAGGTLAFCAVAVVLSRTGLGTKHFFDGMLRFAMPNESEARLA
                     LEKTLRGNLKTFVLVTMREVDGGERIDVAYQVRLKPSRPAAEVIVELGKIKGLSDVQF
                     MMQDATTEM"
     gene            14529..15560
                     /locus_tag="PLT31_01555"
     CDS             14529..15560
                     /locus_tag="PLT31_01555"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HPW93849.1"
                     /translation="MFFAWILSIVGIVALGFLYQGTSVMFSGIAEATETTVSVQSAVE
                     VVKMHVVVGQEIKDGDTLVELSRGDLVLRMNEVKRELDALEGRGSINTATIDQKVAEV
                     QADLATKKNMILFEIEKLKSEYQKNLEMASKLKSLSSSVVAKADSNDAMKMRIRNLEQ
                     ELKMVEANASAQIGILRGSKGLQRTSSASEAEALRRELELLQKEQDDLVITSKGSWVV
                     ASVNVRDGEKISSFSPMITLTRKSPSLVRGYIHEKMYKRVGVGTTVTIISQADGKKLE
                     GEIIGLSSRIVEFPIRLRKIPESVIYGREVIIRIPEDNALLLGEMVSISEVPVIKKIL
                     QHDESRNNP"
     gene            15557..16906
                     /locus_tag="PLT31_01560"
     CDS             15557..16906
                     /locus_tag="PLT31_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93850.1"
                     /translation="MRKIGFVFLFMTSTLMATPLTWDRLVKSADEDPVLKTANRRIGS
                     ISSGPTTKLWDDLELRYNLGGFGFLEHDIELRLKPNSVGEQKATRKYWKAQKSYQQAR
                     YNVDKSYLIYDRYERALRYVIRQKMATINEELYQVNQDRITVLHARAGSETFNAVDLM
                     GAIEKTAALRAELIADSTALRDTEMKLRSWVEFDSISLDTSWLPTMEELNATLQQQSH
                     VDSTFPLIAMAKGKWNVNEERLIQENAGDRDFISHIGLGYKHVIAEKKYKWIDEVDYI
                     GDRDQYSDWKESCGSTPGCEAWLLSRSEDDRRTIDKFYLNIGIKLPFFSNNNDDLMRR
                     QISVLDAESDYLDEYRDLDQKVARLHEEIVALIAQWKVQKDFVAQVDAGSVFQEFAAM
                     AGSDPLLLLRAKESALESTLKAVKLEYDIFQRYLILLDYAGVFSRSDISNHLKAGIK"
     gene            16906..17727
                     /locus_tag="PLT31_01565"
     CDS             16906..17727
                     /locus_tag="PLT31_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HPW93851.1"
                     /translation="MAGARGFSTLERFELKYHIPVSWADRIGSLIAPYCEEDYYSKIT
                     PGGFYWITNLYLDSPRWTFLNWKKAGLLDRFNMRIRTYGEHPDPNGVFHFEVKRKVKS
                     VCYKSRATIKGANPSVVFNEPPEQWHAKSDRDVKYLKDYLYKTALHNAAPRLLTQYKR
                     RAWFGVTEEYSRVTIDTGMRFREENGFDYSVDPKYMHSTGLPKFFSPACDAVLELKCP
                     CSQVPFWMIDLIKILNLKQGGFSKFGNAAAEWKRVYENPRGFKTPLYTSLAGNFF"
     gene            17739..>18402
                     /locus_tag="PLT31_01570"
     CDS             17739..>18402
                     /locus_tag="PLT31_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93852.1"
                     /translation="MRKSFIKTTVLFFSLFYLVSCSDEPTKTQKENFPVIGCTEIMYN
                     TGTDSLEFVELKIVSGPSIVDMATATLRIEGAFNYTFPSEPLDTGEYIVVTNDPVLFH
                     SKYPQFTGRLFGPWDLDEDGTLPRLPAEGDNIEIKLSGKGDVSCSFSGEPPWSSLANG
                     KGRSLVFLGGNNNPSHSEAWAPHTLDGGNPGGPDTYIPQLSVRINEVAPILDGKASWV
                     EIY"
BASE COUNT         5305 a         3121 c         3903 g         6073 t
ORIGIN      
        1 atcgctaaga tgcatggcac aaaacaaatg actcttccat gccctatgtg taatgtgatt
       61 aacggtggag ctcatagctc tgctcctatt gatttccaag aattcatggt agctccagtt
      121 ggtgcaaaga ctttctctaa aggtcttcaa atggtgactg aaattttcca cgctcttaag
      181 aaagttctta aggatggtgg ttatgatacc actgttggtg atgaaggtgg ctttgctcca
      241 ggcgttgctt tgaagaaaga tccaaaatca aaattcggtt ataccattaa aggtgtgatg
      301 actttggaaa atgcattgaa agcattaggt acagctactg aaaatgctgg ttataaattt
      361 ggaaaagaca ttaagattgc tctagacgtt gcttcttctg agttctgtga caagaaaggt
      421 gcaaacgaaa cttatacatt caagaagtcc acaaacaaaa cacttaccag caaacaaatg
      481 gtttcttatt atgaaaaact ttgcagtaag ttcccaattt tctccattga agacggtctt
      541 gatgaggcgg actgggctgg ctggaaggtc ttaacagaca aattaggtgc taaagtaaat
      601 ttagtgggtg acgatttatt tgttacaaat ccaactattt tcaacgaagg tattgaagct
      661 ggtattgcca atgcgatttt aattaaggta aaccaagtgg gttcagtttc tgaaactctt
      721 gctgctatta aacgcgctca agatgaaggt tatgctcctg tcgtttctca tcgttctggt
      781 gaaacagaag acactttcat tgcagatctt gctgtgggta ctggtgctgg tcaaattaag
      841 actggttcgc tctctcgtac agaccgcgtc tgcaagtaca accgccttct ccgtattgaa
      901 gaagaactcg gaaaagacgc tgtttacgca ggtgatcctc gcaaatagtt aaatgtaaac
      961 aaaaatatac aatgctcttt acttctatgg gtgaagagca tttttgtata cttttttgcc
     1021 gttgattttc tataaaacgg tacatttttt gatacattaa attatattag tcttgttttt
     1081 cattcgagtt atctaaaact cttaaaagag gatataatgg tagatcttaa aacgattgtt
     1141 cgagcagctt ttactattcc tgccctttgt gcattttttg taggctgtgg tggacaacag
     1201 gattcatcat cagaaggttc tttaccacgt caagagacgc tttatctttc cggtcaacaa
     1261 tggggttctc ccgctacttt caatccgctt gctgaaagct ggatggcagc ttggcctgta
     1321 ggcggacgtt ttaatttaat gtacgaacct ctgattacat acaacacgtt aaatggtaat
     1381 tttgaaccac ttttgggtac cttagttgat agtttatcaa ataatgatag tatcgttgtg
     1441 gatctgaatc ctgcggcaaa atggagtgat ggaaagcctg ttacatccaa agacgttaag
     1501 tttatttttg agttaggttt caattatcct ggagctgtca caagttttgt aacagaacaa
     1561 attacaggaa ttaatgtcga taccgtttat tctgttgatt ctgcatcaat gaaaacaacg
     1621 atgttaaatg aacgtctctc tttcattgtt gcaaaaaata aaagaaataa tcctttaagt
     1681 gttaaagatg cattacaggc gattcgtatc gttcctgagc acgttttctc taagcttctt
     1741 gcagagaata aaaattccct tgatgaaatc aagaaaatga tgatggataa agatcctgtt
     1801 gtttctggac cttataattt gcaatcttat tctccaacta aaattatcct aaaacgtcgt
     1861 gatgattatt ggggaaatgc ggctttgcat gatggtaaat tgcctgcacc aaagtatgta
     1921 gttcatccta tttacaagag taatgaacat agcactattg ctctccgcga gggttcttta
     1981 gatgcttcta tgagttttgt tcctcgtatt tggcacaaga aaatctctgg tgtaaaaacg
     2041 tggtataacg atcctcctta ttttgtccct ggcgcaatgc caatgctctt tatcaatacg
     2101 acaaaagccc cattagataa taaaatattc cgccgtgcta ttgcaacagc aatagactac
     2161 gtcgctattc gtatgtttgc tgtttctaat tatacctctg aaattatctc tggtttaatc
     2221 atgcctactt ctttagaaaa agaatttatc aataaagaag attgtgataa gtatggaatt
     2281 gatttttcta ttgctggaga cgaaaagtta aaggctgttc aaacaatgat tcagcaggct
     2341 ggttttaaat ctgtatttga aaaagatggc tctctatctc acatggaaaa tgccaagggt
     2401 gaaaaaatgc ctacgatgtt cattactagc cctgcaggtt ggactgactg ggaagctatt
     2461 gtaacaattg ctgttgaagg aatgagaaaa gcaggtattg atgttcgcga aggatttgta
     2521 gatggtggtc agtactggcc tgctatggga acaggaaatt ttgatttgat tatgcataag
     2581 cctgtagccg atgtttctcc ttctcttcca tggagccgct ttgaagccgt aatgtcttct
     2641 agagactggc gtcctattgg tgattgggct ggtacaaata ttggacgtta taacgctcct
     2701 ggttctaaag aattccgccc agaaattgat agcttattgg ctttgattcc tctcatgacg
     2761 gataaagaac aaattaagga tgcgtatcat cagttgaatc gtatttttat ggaagatcaa
     2821 ccggctattc ctttgaccta tttgccggaa caattctatg aattcagtga tcaagtctgg
     2881 accaattggc ctactgaaaa aaatccgtat gcacctccac aattaccttg gattgcttcc
     2941 ggaacaaaaa ttctttggaa tctccagctt tcaaaataag gacgagtaat gcttaaacag
     3001 tttcccaccc tccgttatgt cctacaaaaa gggttctggt accttttaac atttgttgtc
     3061 gctgtggcta ttaacttcgc cttacctcgt ttaggtgaaa ataacccagt ggatattatt
     3121 atgggtaagg ctgctcaagg cctttcccct gatgaagcaa aaaagaagaa ggaatctctt
     3181 ttaatctcat ttggtatggc tgaactcaat gatgacggct ctgtcaaata tgaaccagta
     3241 atcggtccta atggccaacc attaacagaa aagaaacctg tcttagatga atcaggaaat
     3301 cttattatta aaatggttcc tgctttagac gcttctggta aaccgattgt tgaaaagaaa
     3361 cctgttcttg attcagcggg taatcctatg actgaaatgg ttcctgttct tgataagaac
     3421 ggtaagccag tcaagaagaa aggaaaagtt gttgaagaag cggttccagt gatgcaagac
     3481 gtcactatag aaacacctga tactttgaag caagatgttg ttgttttgca aaaagatcca
     3541 aaattagctt caggcctttc tcagtttttt gtctacatca aaaacgtgtt ccgtggagac
     3601 tttggtcttt cctatagtca gtaccctaaa ccagtgattg atattatcaa ggaaagttta
     3661 ccttggacga ttgctattca attaccaaca attatcttag gatggattat tggtaactta
     3721 ttaggtgctt ttgctgctta taaacgtgga atttttgaca aggtcttttt cccattagcg
     3781 atgttcttaa atggtgtacc tttctttgtc tttggtatgc ttttggtcgc tttattctct
     3841 attactttag gatggttccc tgctatgggt gcatatggcc cagaaaaggt gcctagtttc
     3901 tctttagatt tccttaaatc tttaggttac tattatgtct taccattctt ctctgtgttc
     3961 cctattctat taagtggtca agcaactggt atgcgctcca tgagtattta tgaacttggc
     4021 acagactata tgaagtatgc aaaatggtta ggtcttcgtg aaggaaaaat cattggttat
     4081 atcttccgta atgcgatgct cccacaatta actggattag ctcaatcatt gggaacaatg
     4141 gttggtggtg ctttaattac agaaatgatt ttctcttatc ctggcttagg tatggctatg
     4201 cttacagcta ttcagcaaaa tgattacgcc actattcagg gttgtacttt gatgatatcc
     4261 acatgcgtat tactagccaa ttttggagtg gacgttttga ttgctgtgtt tgatcctcgt
     4321 gtcaaagcgg gcttgcaaat gggagataaa taaccatggt taaattatta aaaaaccttc
     4381 ttaaatcacc aatgtttgtg attggtgttt caatcttctt gatcactctt gcgattgcat
     4441 ttttaggccc tgtcttttat gatgtggata ctaatgctcg tgatattgtg gcaggtcctt
     4501 atgctccttc cagtgccgag cacattttag gaacagatca tttgggtcga gattatatgt
     4561 ctttattgat tagtggatta cgctcatctc tttatgttgg ttttgttgct ggtatcattg
     4621 ctacgactct aggagttttt atcgggctct ttggaggctt ccgtggcggt tgggtcgatg
     4681 aagtgttaaa tatgggtaca aacctattta tcgtgattcc atccttcgtg attctagttt
     4741 taatcagctc tgctgtaaaa gatggacgtt ctcttacttt aattggatta atcattggtc
     4801 taactacttg gagctggtct gctcgtgctg tgcgtgctca ggcctcttct ttaagaagcc
     4861 gtgaccatat tgctttagct cgaattaatg gtgcaagttc ctttaccatt gtgattaagc
     4921 acgttcttcc ttatttgtta tcatatgtat ttatggtgtt tatcatgcag atctcttctg
     4981 gaattctttc agaagcatct atttcgatga ttggtcttgg gcctcttgat tctacatcgc
     5041 ttggaattat tctaaaccaa gctaaagata acggggctct atctgatggt atttggattg
     5101 catttcttcc agcaacctta attatcacat tgactgtttt tgccctttat ttagtgaata
     5161 catcaatgga aggtgtcttt aacccgcggc ttcgcaagta aggtggtata ggtatcttat
     5221 gtctgagaat atttttgaag tagaaaatct tagcctccat tatttgggcc gttttggcga
     5281 taaaactcac gccgttacag atgtttcttt taatatgaaa caaggcgaaa tcttaggtat
     5341 tgcaggtgaa tctggctgcg gtaaatccac tcttgttagt ggtcttatgg gaatgtgtat
     5401 tcctcctctc tatccaacaa gtggagatgt ccgtgtcaga ggcgaatctt tgatgaatag
     5461 aaccgtcgaa gacatccgtt caaatgtgct agctcaaaaa gtatccatga ttcctcaggg
     5521 ggcttttaat gcactgaatc ctgttcgtaa gattaaggat attgcagcag atgtgattgc
     5581 ggctcatcag aagcctggtt catctatgaa agctagtgaa atatacgaac gtcttcgttc
     5641 acgttttgat ttatttggaa tggatacaga tcgtgttttg aattccttcc caatacaatt
     5701 aacagctggt gaacgtcaac gttctgtgat tggtatttca acacttttga accctcaaat
     5761 ggtgattgct gatgaaccaa catcagcact agatgtcagc acacaaaaag aagtcattaa
     5821 gatgatcttt gatcttttag ataaagggat cttttctaca atgattttca ttacacatga
     5881 attacctctt ctttatcatg tggctgacaa tatcgctatt atgtatgctg gtgaaattgt
     5941 cgaaaaagga accgcagaac aagtagtgaa agatcctcgt catccttata caaaggcgtt
     6001 gatgggagct atgcttagtt cagagaaaag ccaacgtact agaaagccag tcgctattga
     6061 aggggctcct cctagtttag ctaagaaaat cgtaggctgc cgctttgcag aaagatgctc
     6121 ttttgcacgt cctgaatgta agaaaaatac ccagaatata cgaatggtcg gtgaccgtga
     6181 ggtgaggtgc gaatatgctc agtaataagt cgattgtttt ttcagcaaaa aacatttcaa
     6241 aaacttttgg tgccggtaaa aaaattacta cagcagtgaa taatgtttct tttgatattt
     6301 atgacggaga aatcatttcc attgtgggtg gttctggttg tggtaaaagt gttcttgcaa
     6361 aaatcatgct tggtctttat ggcccttcca aaggcaagtt ccattataac ggtaaaaaaa
     6421 ttaccaacct aaaaaaacac tggaatgatg ttcaatctgt tttccaggat ccgtttagct
     6481 gttttaacca gttcttcact attcgctctc agttggaaga tgcttttaac atcttgaaaa
     6541 agaagccttc taaagaagaa attaagcgtc gtgtagatga aggtctgctt gctgtgaatg
     6601 tgacgccaag tgatatcgaa ggaaagtatt cttttgagct ttctggcggt cagatgcagc
     6661 gtatgttgct tgctcgtatc tttgctttgc gtccaaaagt actcattgca gatgaaccaa
     6721 catccatggt ggatgcttgt gtacgtgcaa acattcttga ttatttgatg aaacttaaag
     6781 aagagcttca aatgaccatc gttttcatta ctcatgatat tggacttgct aactatgtta
     6841 gtgatcgtat ctttattatg catgatggta tgattgttaa ttctggaact ccagaagaag
     6901 ttcttgataa tactcgtgaa actcatacct taaagctttt ggacgatatt cctgaagtgc
     6961 ataagaaaga gtggatcaag aatagtcata gatctaagaa caaagaagca tcctaatttt
     7021 tggaaaaata aaaaggcctg aaagaaatac tttcaggcct tttttatggt tagatttttt
     7081 tagaaaaccg tttgtgattt aatgtgtatt cacttttgat cgaaaaggta aaagttactt
     7141 ctgtatcttt tttacaaatt gacggcaagt ggcttcagga tctggtgtgt tgaaaatagc
     7201 actaccgaca acaaaaatat ttgcccctgc ttcttgagcc agttgaatat tatcgagttt
     7261 gatccctcca tcaatttgaa tattcatttc aggttttaaa tcacgaagtc ttttaatttt
     7321 atctagggta tatggaatta aaccttgccc tccaaaccct ggatttactg acatgatgag
     7381 aaccatatca acgatatcaa gaacgtttaa tatggattca ataggagtcg ctggattaat
     7441 agaaactgcg ggtttgcatc ctaattcgat aattctatgt aaaagtcgat ctaagtggaa
     7501 agtcgtttct gcatgaacgc aaatgtagtg tgctccagct tttgcaaagg cttccacata
     7561 attgtctgga tttgtgatca ttaaatgaca gtccagagga acagatgttc ctttttttat
     7621 gttatctgca atgccaggtc caaagctaat gtttggtaca aagtggccat ccatgatatc
     7681 aagatgaatt aagcctgcac cgcctttttc aatcgcggag aggctttttc caagttctaa
     7741 aaaattagcg tttaatacgc tcggggctat gatctgtggt atactcataa ataaaatata
     7801 gtcagtttac gttcttttta ttactattta cttaagaaac cactcaaagg agattgcaag
     7861 atggctattt caaaaaaaac aactgctgct acgccagcaa aaaaagctgc ttgtggagca
     7921 aaaaaaacag ttgatacaaa aaaagtaaag gcaactgtaa aaaaagaaac aaaagttgaa
     7981 accaaggttt ctgcaaaagc agcaaaaacc gtgaaagctg ctccagcaaa aaaagaagca
     8041 agcaaagatg ttgtttttag tattgatgct cctttggcaa caacagtcgc agtggctggt
     8101 tgctttaaca attggagcgt gactaagaat aaattgaaaa aagacaagaa aggtgtgtgg
     8161 tctgtaaaga tccctcttgc tgtaggtcgc tatgaataca agtttgtatg cgatggtgtc
     8221 aattgggatg aaggtgaaaa caaagtaaaa aatgtctgat cattttattc ctgaatcagt
     8281 ttttcaaagg ggcgctgaat cttttggcac tcctttttgg ctttatgacc gagaaactat
     8341 tgaagctcgt gttcgtgaag taaaagtttt tgatgtagtt cgctatgctc aaaaggcatg
     8401 ttctaatttg tcgattcttt ctttaatgag aaagttaggc gttgtcgtgg atgctgtgag
     8461 tgctggtgaa attgttcgcg cattgcgtgc tggttatcaa ggtggagtag gtaaacaaac
     8521 atctcagatt gtttatactg cagacatttt tgatgcagac gctcttgccc ttgttaaaga
     8581 acatcatttg gctgtaaata tcggctctcc agatatgatt cagcagcttg cggattatgg
     8641 tgttcgaaca gaattgatgc ttcgtgtgaa tccaggcttt ggtcatggtc attctcgtaa
     8701 aacaaatacg ggaggagacc tttctaagca tggcgtttgg cacgaacaaa ttaaggattg
     8761 tattaagtta gctcagaaaa atggtatgtg gattactggt ctacatatgc atatcggttc
     8821 aggatctgat tttgggcatt tagctcaggt atgtgatgcc atgtttgatg ctgcaaggct
     8881 tcttggtggc cacttaaaaa cgatctctgc aggcggtggt ttacctgtta gttatcatga
     8941 aaatgaaaaa aatgatagaa tagatattaa cgcctattat aagttatggg ataacactcg
     9001 taaacgtata gagcagtctg tgggtcattc tgtttcatta gaagtggaac ctggtcgtta
     9061 tttgattgct gaaagtggtt ttttagtttc agaaattcgt gcagtgaaaa agcaaggaac
     9121 taatactttt tatttagtca atgctggttt tactgattta gtgcgccctt ctttttatgg
     9181 ctcttatcat gaaatttctg taatagctaa agatcatcgt ccgttaagcg aacgtcaaca
     9241 agtgattgta ggcggtcctt tgtgtgaatc aggagatgtt tttacacaag aagaaggtgg
     9301 ctttgttgtc tcacgcgagc ttcctgtggc acaagtagga gatctattag tgcttcatga
     9361 tgtgggggct tatggctctg cgatgtctag taactataat agtcgtttgc tcgctgcaga
     9421 aatactctac tctaaagggt ctcttgaggt cattcgagaa aggcaatcgt ttgaacagat
     9481 aatgcaaaat gatcgaattg tagatttata aaagatatca taataattat ttttttgaat
     9541 tcatttttaa agcccagccc tttttggaca gggctttttt tatgtacagg tttttagaac
     9601 actataaata aaaaatgata ttgttttgtt ttctttgaat taaaaagaaa atatttatgt
     9661 ttaaagagct tatcattaat aagagtattt caaatgttac gcgtcagtct tttattctct
     9721 ttgattttat tattcgtttt acctacgtat gcggtaaaac cctcgaactc ttacaaagat
     9781 ccacgagata aacaaaagta tcgtaccatt aaagtaggcg atcgaatatg gtttgcagat
     9841 aatttgaact ataaaatgga tgggagtttt tgttataaag aggatgaaag taattgtcag
     9901 gcttatggtc gtctttatac ctgggatgcc gctataaagg cctgccccgt aggatatcat
     9961 ctaccaacgc atggtgattt tgaatcatta tggcaagcgg ctggggctga ttttaatgca
    10021 gcttatttat taaaagcgga gtatggttgg tctggcgaaa cgaatggtaa tgatacgtta
    10081 aaatttagtg ctatgcctgc tggtaatcga tttgatgatg aaacctatgg aaacctaagt
    10141 aagtttgcat tcttttggag ttctgaggct agtacagaaa aacaaaacgc ccgtgtttgg
    10201 tatatgacct caaaatcaat ggcttttagc tttatgaata agccgaaaat ctatggtttt
    10261 tctgttcgtt gtgttcaatc ctcaaaaaag taagcgaaaa atatctaaaa acggctaaaa
    10321 ggctttttta aatattattt cgcacaatat ctcatttttt attacgatta tggtttaaaa
    10381 cttaggatgc tatttgtatt tttagaaaga ttgttcatct tatacaaggc tctattatgt
    10441 cagatcgttt tgttgttaca ggtactttta ctgatgatcc atttgcaata gatgtcgctc
    10501 aatatatagg tcttcgagaa gatatctctg ataccgtagc actgaaaaca tttgctaatt
    10561 cagaattttg tcctcgctat gttttagatg ccgatgatat tgagcatatc ggacgtcgtt
    10621 tgcatggtaa aattgtttta atttgtactg tctccactca cgatcgtagc agaaatgatt
    10681 tggctatgcg taactgtatt cttgctcgag cagcaaaaga taatggagct gaacgtgtgg
    10741 ttttagttga gcctgatttg ttttatagcg ctcaagaccg aggccctcat cgttttggtg
    10801 aattagaagc tgatcgtgaa atctctgact taaaaaaatt tgatggtcaa gcttttactt
    10861 cactattgta cgctcaactg ttaaagactt ctggagtcga tgcggttgtc actgttcata
    10921 atcacagcgt gaaggttcaa aaattattta atgatgtttt tgaaggccac ttccataatt
    10981 tgattccaac agaagtatat gctcactata ttaagacatc taattttgta cagacaggaa
    11041 aggatggaga taatctagtg attgttgctc cagataaagg ggctactcca tttatgaata
    11101 acatgtggga tgcgctcgaa ttacctttgt gtaagcgcgt ggtcatgaat aaggttcgta
    11161 gtggtgaacg agaagtgtca atgactttaa gtgatcatag tgaagttgga atggattatt
    11221 tagaaggcaa agatgttatt gtttttgacg atatggttcg tacaggaacg acgattgttc
    11281 aatgttgcga gtatttaaaa acaggtcatc ctaaccgcgt ctgctttgga gtgacgcatt
    11341 ttcatacaag tccagaagca cgtgaaaaat taaatagcca attcattgat gaaattttaa
    11401 cgactggaac tcttcctgat attatgaatc gagattgtca gggacgttta cgtcgcaagt
    11461 taactgtcat taagcttggc aagtggatgg ctcgccatat tttacagatg tacgggcttg
    11521 atgatggccg atttgaaaag aatttttata agatcgatat gagttcaaaa aacccacgtt
    11581 ggccgccccc acatttatat taaaccgctc acttttattc acctcttttc tttaatttta
    11641 tggcaaaaaa aaacagtgaa gtgactgatg atttatttcc aaaaggccgt atagatagag
    11701 tcattgctcc tatctacagg tacttacaag ttgaaacgac ttcgggagta attttaatta
    11761 tagccacagc gcttgcgctt ttttgggcga attttgcagg gaattcttac catgctcttt
    11821 gggaaatgcc tcttggcatt tcaataggcc gttttgattt tacaagatca cttcattggt
    11881 ggattaacga tggcttaatg acattattct tttttgttgt gggcctagag gtgaagggtg
    11941 agatcattaa cggtgagctt aaaaacccta aaacagcatc tttaccaatt atcgctgctt
    12001 taggcggtat gattatacct gcggtaattt atttaatgac ttgtcctcca ggaactaccg
    12061 ctggttgggg agttcctatg gctacagata tcgcttttgt agttggctgt atggctattt
    12121 taggaaatcg aattcccaaa ggcttgcata taatgatttt gacgcttgcg attgcggatg
    12181 atataggtgc cattattgtg attgcattag ggtattcagg tacgattgac ggtcattatt
    12241 tattgatggc tttagggctt ttagctttaa cgcatgtctt tttcagaata ggtattcgta
    12301 atttaatcgt tcatgttcta ttaggtgcgg ttatttggtt tgcttttgtc aatagcggta
    12361 ttcatcctac tcttgctgga gtggctcttg gattattaac gcctgcgatt ccttgggtta
    12421 aaaagggagc tgttaacagt tatgtgcata aagtgaatga tttcttggct ggagggccta
    12481 gtccagaaaa cgtgacacaa tacgatattt ttagaacgct acagaagtgc tcttgcgaaa
    12541 gtgtagcaat gcaagaacgc ttattgcatg ttttgcatcc ttgggtgagt tatgttatta
    12601 tgcctctttt tgctcttgcc aatgcaggcg ttataattcg aggagattct tttagtggag
    12661 tgactttatc tattgggcta ggtcttgtgc ttggtaaacc aataggcatt ttcttgttct
    12721 cttttgctgc tgtcaaaatg cgcatagcca aattaccagc aggggtctca ttaaaaatgc
    12781 ttctaggtgg aggcgctttg gctggaatcg gttttacaat gtctttattt gtaagcgagc
    12841 ttgcttttgt gaatagtcgt tctttattag atggagcgaa gattgggatt ttgttcggta
    12901 gcttcctttc agctttaatt gggatgttaa ttatctatgc ccataaaaaa tcacccttaa
    12961 ataaagaata aaaagaaata aaaaagcctc ctttcggagg cgtttttttt accagttttc
    13021 accaagaagt tcgaagtgtg cttgtgggtg taagcaagcg gggcagagct caggagcttc
    13081 tgttccttca tgaagaaaac cacaattgcg gcaacgccag acaaccttct cattacgctt
    13141 aaatacctta ccttccatta aattattata gaggtctcgg tagcgctttt cgtgctgttt
    13201 ttctgcaata gaaatcattt caaatgcctt ggcaatttca gggaagcctt cttcgcgagc
    13261 tattttagca aaagcagggt agagatcaga ccactcttca ttttcacctt cagcagcagc
    13321 ctttaagttg tctagtgtat ttgatgatac accagcaggg tatgtggctg taatttcaac
    13381 atttccgcct tcaagaaact tccaaaaacg cttagcgtgt tctttttctt gattcgctgt
    13441 ttcttcaaaa atgtcagcga tttgaacaag cccttctttt tttgctacac cagaaaaata
    13501 ggtatagcga tttcgtgctt gcgcttctcc agcaaatgct tttagaaggt ttttttctgt
    13561 ttcggttcct tttatacttt ttgacataga gtctcctata atgttatttt ctattgtatt
    13621 atatacgttt ttttacaacg ataaaatagt aggatctata aaaaaacggg actttttata
    13681 ctaaaaagct tattttatga ctgtattttt ttttgttttt ttcattttac acaaagaatg
    13741 ctgatttctt tccatttaat tggtagtttt gattttaaca aagttgccgt ggagttaaac
    13801 tatgcttgat cttcttgctg tacagtctag tactgcgcat ccaacagtca ttacaatggt
    13861 atatacattg cttttggctt tcgttctttc ttccttaatt ggttatactt acgaaaaaac
    13921 ttttttaggc ctttcttatt ctagaaattt tgtccaagct cttgttttga gctctgttgt
    13981 tgcagcaacg gtgatgcaag ccattggtga taatgtcggg cgtggtcttg gtatgttagg
    14041 agccttatct attgtacgat tccgtactag tttcaaagat ccacgagata ttatgttctt
    14101 atttgcggca ctgggggctg gtattggctg cggtgtattt gcctgggggg tcgcagctgg
    14161 aggtaccctt gcattttgtg ccgttgcagt cgttttaagt cgaactggtt taggaacaaa
    14221 gcattttttt gatggcatgc ttcgttttgc catgcctaac gaatctgaag cacgtcttgc
    14281 tttagaaaag actcttcgtg gtaatttaaa aacatttgtt ttggttacga tgagggaagt
    14341 ggatggcgga gaacgaattg atgttgctta tcaagttcga ttaaagccta gtcgccctgc
    14401 tgcggaagtg attgtggaat taggtaaaat aaaaggctta agcgacgttc aatttatgat
    14461 gcaagatgca acgactgaga tgtgatatga ctaaaagcac gatgaaatat tatatactga
    14521 agaacaaatt gttttttgca tggattttat ccattgtcgg aattgtggct cttggttttt
    14581 tatatcaagg aacaagtgtc atgttttcag gaattgctga agctacagaa acaacagtga
    14641 gtgtgcaatc tgcggtggaa gtggtgaaga tgcatgttgt tgttgggcaa gaaataaaag
    14701 atggtgatac cttagtggaa ttgagccgag gtgatcttgt tcttcgaatg aatgaagtca
    14761 agcgagaatt agatgctctt gaagggcgtg gaagcattaa tactgcgacc atcgatcaga
    14821 aggtagcaga agttcaagct gatttagcaa cgaaaaagaa tatgatcctt tttgaaattg
    14881 aaaagctcaa atcggagtat cagaaaaatt tggagatggc ttccaaatta aaaagtttgt
    14941 cttctagtgt agtggcgaaa gcggattcga atgatgcaat gaaaatgcgt attcgtaatt
    15001 tagaacaaga attaaaaatg gtagaagcca atgctagtgc ccaaattggt attcttcgtg
    15061 gtagtaaagg cttacaaaga acaagtagcg cttcagaagc agaagccctt cgtcgagaac
    15121 ttgaattatt gcaaaaagaa caagacgatc ttgtcatcac tagtaaagga agttgggtgg
    15181 ttgcaagtgt aaatgttcgc gatggagaaa aaatttcaag cttttcccct atgattacat
    15241 taacgcgtaa atcgccttct ttagttcgtg gctatattca cgaaaaaatg tataagaggg
    15301 ttggtgttgg tacaacagtc actattattt ctcaagcaga tggaaaaaag ctcgaaggtg
    15361 aaatcattgg tcttagtagt cgaattgtag aatttcctat tcgattaaga aaaattcctg
    15421 aaagtgttat ttatggaaga gaagtgatta ttcgaattcc tgaagacaat gctttgcttt
    15481 taggtgaaat ggtttcaatc tcagaagtac cagttattaa aaaaattttg cagcatgatg
    15541 agtcgaggaa caatccatga gaaaaatagg atttgtattc ttgttcatga cttcgacttt
    15601 aatggcgact cctttaactt gggatcgtct tgttaagtct gccgatgaag atcccgtgtt
    15661 aaaaacagca aatcgacgaa tcggatccat ttcttcaggc ccaaccacaa agctttggga
    15721 cgatttagaa cttcgctata atttaggtgg gttcggcttt ttagaacacg atatagaatt
    15781 acgcttgaaa ccaaattctg ttggtgaaca aaaggcaaca cgaaagtatt ggaaagctca
    15841 gaaaagttat cagcaagcac gttataatgt tgataaatct tatttaatct atgatcgata
    15901 tgaaagagct cttcgttatg tcattcgtca aaaaatggca acgattaatg aagaattgta
    15961 tcaagtgaat caagatcgaa ttacagtact tcatgcaaga gctggttctg aaacattcaa
    16021 tgcggttgat ttgatgggtg ccattgaaaa aacagcagcc ttaagagcgg agcttattgc
    16081 agatagtaca gcccttcgtg atacagaaat gaaattgcgt tcttgggttg agtttgattc
    16141 tatttcttta gatacttctt ggctccccac tatggaagaa ttgaatgcga ctctacagca
    16201 acaatctcat gtagacagta cgttcccatt aattgcaatg gcaaaaggaa agtggaatgt
    16261 caatgaagag cgtcttatac aagaaaatgc gggtgatcga gattttattt cccatattgg
    16321 tttaggttat aaacatgtga ttgctgaaaa gaaatacaag tggattgatg aagtcgatta
    16381 tattggtgat cgcgatcaat attctgattg gaaagaatct tgtggctcca ctcctggttg
    16441 cgaggcttgg ttactttctc gttcagaaga tgatcgccgt acgattgata agttttattt
    16501 gaatattgga ataaaactac ccttcttttc aaataataat gacgatttaa tgcgccgtca
    16561 aatttctgtt ttagatgctg aaagcgatta tttagacgag tatagagatt tagatcagaa
    16621 agtagctcgt ttacatgaag aaatagtggc tttaattgct cagtggaaag ttcaaaagga
    16681 ttttgtggct caagtggatg ctgggagtgt tttccaagag tttgctgcta tggctggctc
    16741 agatccttta cttcttttac gtgcaaaaga aagtgcttta gaaagcacct taaaagcagt
    16801 caagttagaa tatgacattt ttcaaagata tctaattcta ttggactatg caggtgtatt
    16861 ttctagaagc gatatcagca atcatcttaa agctggaata aaataatggc aggagctcgt
    16921 ggctttagta cgctcgaacg atttgaactg aaatatcata ttccagtttc ttgggctgat
    16981 cgtattggaa gtttaattgc tccatattgt gaagaagact attattctaa aattactcct
    17041 ggtggctttt attggattac aaacctttat ttggattcgc ctcgttggac tttcctaaac
    17101 tggaaaaaag caggtctact agatcgcttt aacatgagaa ttcgcaccta tggagaacat
    17161 cctgatccta acggggtttt tcattttgaa gtcaaaagaa aggtgaaatc cgtctgttat
    17221 aaaagtagag cgactattaa aggggctaat ccatcagttg tttttaatga gcctccagag
    17281 caatggcatg ctaaatctga tcgagatgtt aaatacctta aagattatct ttataaaaca
    17341 gctcttcata atgcggcgcc acgtttatta acccaataca aacgaagagc ttggtttgga
    17401 gtcactgaag aatactcaag agtcacgatt gatactggca tgcgatttcg tgaagaaaat
    17461 ggcttcgatt attccgtaga tcccaaatac atgcattcta cgggattacc taaatttttc
    17521 tcacccgctt gtgacgctgt tttggaatta aaatgccctt gttcacaagt gcctttttgg
    17581 atgattgatt tgattaaaat attaaattta aaacaaggtg gattttctaa attcggtaat
    17641 gctgctgcag agtggaagcg agtatatgaa aatcctagag gattcaaaac ccctctatat
    17701 accagtttag cagggaattt tttctaaagg aactctttat gagaaagtca tttataaaaa
    17761 ccactgtcct tttcttctct cttttttatt tagttagctg ttctgatgag ccaacgaaaa
    17821 ctcaaaaaga aaatttccct gttatagggt gtactgaaat catgtacaac actggaacag
    17881 attctcttga gtttgtggaa ttaaagattg taagtggccc ttctattgta gatatggcta
    17941 ctgctaccct tcgcattgaa ggagcattca actatacgtt cccgagcgaa ccgttagata
    18001 caggtgaata tattgtggtg actaatgatc ctgttttgtt tcattcaaaa taccctcaat
    18061 ttacaggtcg tctttttggc ccttgggatt tagatgagga tggtactctt cccagattac
    18121 ctgctgaagg cgataatatt gaaattaagt tgtctggtaa aggggacgtt tcttgtagct
    18181 ttagtggtga acctccttgg tcaagtttag cgaatggaaa gggccgctca ttagtctttt
    18241 taggaggaaa taataatcca tctcattcag aagcttgggc tcctcatact ttagatggcg
    18301 gtaatccagg tggaccagat acatatattc ctcaattaag cgttcgtatt aatgaagtgg
    18361 ctcctatatt agacggaaaa gctagttggg tggaaattta ta
//
LOCUS       DAOOBB010000016        17609 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_4215_length_17609_cov_5.777430, whole genome shotgun sequence.
ACCESSION   DAOOBB010000016 DAOOBB010000000
VERSION     DAOOBB010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 17609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17609
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4215_length_17609_cov_5.777430"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PLT31_01575"
     CDS             <1..206
                     /locus_tag="PLT31_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93853.1"
                     /translation="SSKVPLDATAKAWASTTAYRANEKLSGEEMASLVNQLLQTEEPL
                     ISPYGKPTLLRIPIEDIHKKFKR"
     gene            complement(159..1337)
                     /locus_tag="PLT31_01580"
     CDS             complement(159..1337)
                     /locus_tag="PLT31_01580"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93854.1"
                     /translation="MKLIKNRAPKNKSMQNMEHLNQNKKFVFFCAGEDSGDILGAEAV
                     SAMNLWGVEAIGVGGPRMQKEGLKQMACFDDFAISGFKDVLTHYFQLHRSFVLLKKIL
                     ECPNCIAVVLIDYPGFNMRLVELSKKYSKPILYIAPPQIWAWKSKRAYSLQDVTLGVL
                     YDFEREAYKKFDCPSLKIENPFLQGAVSLMKEKPNKGSDFLFLPGSRRHQLHRNMPLY
                     IRIAKQLKKKNPNLQIKFLASRTSLLKEIKKWNFDFEQMVVPENSVERAALFSKAQGV
                     IATPGSGILEASLVGTKALACSRIDPLTYVLGSILVRTPFFTLPNLLLSKRIVPEFLS
                     LVSFFYKKEVLSIVKELEELEKEDFYSNAMALHKTCEGESIKCLSLEFLMNVLNRDSK
                     "
     gene            complement(1341..4904)
                     /gene="dnaE"
                     /locus_tag="PLT31_01585"
     CDS             complement(1341..4904)
                     /gene="dnaE"
                     /locus_tag="PLT31_01585"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPW93855.1"
                     /translation="MSFVHLQTHSEFSLLRSSCRIHELLAEAIKDNQPAVALTDHGNM
                     FGILEFYMQARSLNKSRQKEGLPPIKALLGSHIYVATSTSMTKEEQGYQRLTLIAKTN
                     EGYRNLLKIVSYCYEDAEQWSEVPAVPLEFIREKSKGLIALAGDFFSRFGSDVLNQKE
                     DIANQYLTDLCSIFDSESLFFIVQDQEIEGQKKVNAFIAQASRDLHRGLVAVNNVHYI
                     EKGDAPAHKVLRCIALAEKLQNFSDPLFPTDKFYFKTTEEMVSAFKEYPEAIENTVKI
                     AEQCDITIQTDCGDQFWPQFKFPDEFKDSDDYLGYLCRQKVSNRYPEVTPEVQERMET
                     ELACIREMKVAGYLLIVWDFINWSRENGIPVGPGRGSAAGSIVTYIIGITDIDPLRFD
                     LLFERFLNPERVSMPDIDTDFSDRDRARVIEYVSDQYGKECVTQIVTYGALKMKAVIN
                     DVGRVLGIPISDVRAITKLLPLDLGATLEMAKTGKNKKGAPLEGYNPGPLNELIESRQ
                     NYRDLWNYSEKLENLSRQTGVHAAAVIIAPVAMSDLAPLFRARKEDTPVIMYDKHYAE
                     DIGLLKMDFLGLRNLSIIQDAVALIKQTRNVDLEIGRIPLDDAKTFDLLSKGLTVGVF
                     QFESPGMREYLRKLIPERIEDLIAMNALYRPGPIEQIPHYIARKRGEEPVDCYHEDLE
                     PILSETYGVIVYQEQVMRIAQKLGGYSLGGADIIRRIMAKKKPEEMTKLEPEFMNKCL
                     ERGYKASLVKEIWKVLLPFCGYAFNKSHAAAYAYVAYQTAYLKANYGPEFMAASMTSE
                     ISKTENIVSLLKECSKMDIPYLGPNINSSFATFSVDEQGRILYGLAGIKNVGISIVED
                     IVEERTLHGEYKDIFDLCKRLLNYQASLNEKKSPLSKKVLESLVMSGALDEFPGSRSA
                     LLATIDKAIEVANRYRQNIDCGQVSIFDLGDNDTCIHTAEELEDAEEWTYIELLNKEK
                     DVLGMFISGHPLEEYRPELQGFTTCSLAESEINNSGLLKKSITIGGVITKMRSFETRR
                     GDTLGSGIIQDFQGEIALFLTSDKWEAFRDRVSIDDRVLVQGVLEMQRDDKGLQVVVE
                     KIVPLETVRQKWVKFLHVSFSSLAITEETLSLLNEKLKSYEPFPGESSCQLIFHIESL
                     SGYEHALVAKKIRFKYEQELLVWLKKDFGVTDVWVSNKNRS"
     gene            complement(5131..5949)
                     /gene="tsaA"
                     /locus_tag="PLT31_01590"
     CDS             complement(5131..5949)
                     /gene="tsaA"
                     /locus_tag="PLT31_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HPW93856.1"
                     /translation="MQCDSIGVFHTDFSYKYDVARQGVFFQGHPGIITLKSGFDFEQA
                     LRDLDGFERIWLIFQFHQNQGWRPTAKPPIPPIDRDRVGLFASRSPYRPNAIGLSCVR
                     LLKINGLHLFVDECDLLDDTPILDIKPYIPKVDSFPDAKAGWVDLQESVLWKIESTPL
                     FQEQQDWILKQLSWNMASFAQVQLSSYPLDDTRKRVVKKNNEANEAILAYRTFRILFS
                     YNEEKKEILLKSIDSGYTNEELTSEEDQYKDKAVHRCFRMLFYDKKCQKQNKSI"
     gene            5990..7057
                     /gene="prfA"
                     /locus_tag="PLT31_01595"
     CDS             5990..7057
                     /gene="prfA"
                     /locus_tag="PLT31_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HPW93857.1"
                     /translation="MKDKVLKLIQKYEELESELSRPEVIADQTNYTRLRKTFKGIEKA
                     TTKGREFIQMSNDLTEWQLALNDPDPELNQCAKEEVKSLESKIAELTEELQILMVPKE
                     PWDYRNATLEIRGGTGGDESSLFAGDLFRMYRGYCDRMGWRMSILNASEGTVGGFKEI
                     SAFIEGDSVYGTLKFESGVHRVQRIPDTETQGRVHTSAATVAILPEADEVDMEIRESD
                     IHMDTYRASGAGGQHINKTDSAVRLTHIPTGVVVSCQEERSQLKNRSKAMHMLRSKLL
                     DAAISKKEQEEAASRKALVGTGDRSAKIRTYNYPQNRLTDHRINLTLYSLESVMTGNI
                     QEIIDALQMANAQEKLGKFSV"
     gene            7054..7911
                     /gene="prmC"
                     /locus_tag="PLT31_01600"
     CDS             7054..7911
                     /gene="prmC"
                     /locus_tag="PLT31_01600"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HPW93858.1"
                     /translation="MSTVLEILNVTTEFFKKNKVPDARLDAQYILAHGLKMKRMDLYL
                     NFDRPLTENELTILRPLVARRAKREPLQHIIGNTSFRGFLINCDKRALIPRPETEMMV
                     DFVKEETSLPEEPLIADIGTGTGAIAIALAKEIPSAKIFAVDISKDALDLAISNATQN
                     EVEDKISFFEGDLLSALPAESKLHVIVANLPYIPLKDQASLQEEVRLFDPALALFGGE
                     DGLDLIRRLLRETENRLALGAVIWLEIASEQEEILKNEISQYPWLSYTRAIPDFMGNI
                     RFIELRNKN"
     gene            complement(7918..9849)
                     /locus_tag="PLT31_01605"
     CDS             complement(7918..9849)
                     /locus_tag="PLT31_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW93859.1"
                     /translation="MRSFLHHYLWILVSLFMAVFFQGCTCCAYLNHMFNAERAFEEAS
                     DLRTARLDSIPGDSSLPTMEERKRYDRVIEKGSRVLERFPKNKKRTAEAVFLMGESFR
                     HKQEWNKAITKYDEFERYFADHDSMPVIQYQRAYCLYRNGEYNISRFALEPVLEAGKS
                     HPYYYEGQNLLSLLQEKSDMPNEAIAALENMLADTLGTPYMRAKIHFRLAGLYFKIEN
                     WAKAREHYLAKEIKELTVREKMTASIQAAECLVNQKEYLPAAEEYKKLMVEKDYEESL
                     PEFIVRCGELLKLGDKIDEARSAFRKAIHDYPRTDYTARSYFHLGDDEQVRKRAYEQA
                     LIYYDSSFVSRPSSTWGRDSRERRDALRRMLALQNANEKIKTDTVETTQKRFFDSEFQ
                     IAELFLFKLSEVDSAIQRLNDIIVKADDSIQVLRASYARAFIYDEFKNDPDQAEILYK
                     EIIEKYPNTEYAKQAQVNLGMRVTVKTNEDLAYERFLKAESLWLAAENIPLEKMAEVD
                     SAYRQAFTAYEALYQDYPETQSGIQALFMKGIFYEMNPNTKDSAVFVFRELRSKYGQT
                     AWGKEADRRLQTRVTITDDELKRLRQRIKQNEEHIERLSKQYYESLQAKKTTSPTEEV
                     KTKEEEILENTYNSMYDFE"
     gene            complement(9876..11582)
                     /locus_tag="PLT31_01610"
     CDS             complement(9876..11582)
                     /locus_tag="PLT31_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93860.1"
                     /translation="MRVSSSQARTAAKFFLTLTGGVLLAYFLYFGYQKFGPSKIVLEG
                     VSFGPPAGTVLVRGDVPNLEVGFDQLPFSTRDEVRRAFDLMLQGGSKQAIEIFDAILI
                     SYPELFMVEWGVLHVLWESDSLSSNELLRLEHLIRSVKKKATSASLDLYIDSREAYRL
                     GSLPLALDLARKATEKGASFPDFRLWYGELLQEVNRLSQAIGETRVAIGLTKGTSQKA
                     YENLALLYHQQGNLDSCTSVIEYALTKYPADTKLLLLQGYLSEYNGSFDMAEKNYQRI
                     LALNPNYKEAQAALNTLGEKSPPAHSSSARITPRDKAQLAYDILEPLVATYPENLPLR
                     EALGLAYLKGRSFDKAKQQFKEIQTKDPEYPEIQLRLQEASVTSLRSENDGALTESLN
                     RVMDSIRTTMPSSKHDFSTMLGHYLVRYGASPKEFFTQYAVTNFKEIKKNTWQESFYE
                     PPYYHQYTVLFSKTNQFYGVHVLVIDSSGNSNHLGSAPDIYNRLLKQNSRISGIGIGT
                     GETDCNGTVIDAAVWETLDNFEILARYVSKPSEVRMMRLDKKQISDKMKLCDYLSYLN
                     KY"
     gene            complement(11582..12427)
                     /locus_tag="PLT31_01615"
     CDS             complement(11582..12427)
                     /locus_tag="PLT31_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HPW93861.1"
                     /translation="MSDFNESLYFRDLNRYPTLTPQEEAVLLTIIKKNESEEIRNSAL
                     QRLIRGNLRFVVSVARKYQGRGLSLLDLINEGNLGLFKAAKRFDMEKDVKFISYAVWW
                     IRQSIQKALFEQVGAVRIPPNKLALVNRFKRALVQNGGDYESTIALEEFASCERDIVE
                     VMEKIVDISLDAPIGDDSSSGGDSVSTLMDVLGSEGNQEDELEREERRKLIQETLSSL
                     PQREEEILRMFYGLDSIEDTTLKDIGEDLKLSRERVRQIKNKTLRKLQKSKENKEKLA
                     DFLES"
     gene            complement(12492..12791)
                     /locus_tag="PLT31_01620"
     CDS             complement(12492..12791)
                     /locus_tag="PLT31_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HPW93862.1"
                     /translation="MILQPVKISKTPKNEVRFDWNDGSFSVFNLRALRVACPCALCVD
                     EHTGERILDPNTIPEDISIINLRSIGRYAVGITWTDGHRSGIYPYRQLKELTKTK"
     gene            complement(12788..13882)
                     /locus_tag="PLT31_01625"
     CDS             complement(12788..13882)
                     /locus_tag="PLT31_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HPW93863.1"
                     /translation="MNEKQVLKALEVIIDPDLHRNIVELGFVQNILISENDDVSFDLV
                     LTTPACPLKEKFVVDCKKILSDLGAKSVSIALSGKTQPSQVNLSENTFLKDVSHILAV
                     SSGKGGVGKSTVTANLAMALSLSGARVGILDADIYGPSMGVMFGIEEAPQVHPDKTIS
                     PVLARGGISIVSMSMFADSDKATIWRGPMASQMIQNFLHRVRWGGLDYLLIDFPPGTG
                     DIQLTLTQSCPLSGAVVVTTPQEVALADCRKGLAMFDAVGVAVVGVVENMSYFICDQC
                     EKSHYIFRTGGGEKITKQYGVPLLGKIPLEPSVADAGDLGAPAVLAHPNSVSATEFIR
                     IAEQVVCALSVLSTNNEQVLSNYNYEWSDL"
     gene            complement(13956..15329)
                     /locus_tag="PLT31_01630"
     CDS             complement(13956..15329)
                     /locus_tag="PLT31_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW93864.1"
                     /translation="MIFDDIESLLKEYKQVQILEVPFNPVDPILWSFLKKQERLSVVS
                     ERIQRKIQRALAPFWQQRFISIENKEAPLVRHFLLSPLFFLQTDLVVEDTPFPKTNPA
                     LLSEALQKASLPESVLDRTLLSLSNGELRRILLARVWMENPEWVYFNDLFGGLDAAYR
                     PYLEECVLKMVHSDVKMVIRLAREEEVMPLIPAFVYENGKFVLLEKKREGALKSEQVK
                     KSYSEYEIEKLTSSAVEGKLLFDLKNVNVCFGETEVIQNLSWQVRSGEHWVVMGPNGA
                     GKSTLLALLSADHPQIYKNDIMLLGERPGKGLDVWAHKEKLGFFSPELALQYREELTL
                     QEVLCTGFSTSLGLFKEPVFEEREKANEWLHFLGFEDTKVAFSSLTLIEKRLVLIARA
                     AIKPPSVLILDEPTQGMDAKHRAKIFNWLDYLSSSTTIILVSHYLNEWPKCMTHILHM
                     PKFSMPL"
     gene            15397..16452
                     /locus_tag="PLT31_01635"
     CDS             15397..16452
                     /locus_tag="PLT31_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93865.1"
                     /translation="MIKKISPFLLLICAISAIAQTDSTLSFIAYWKKGDIKYFNISQN
                     KSQHKNDSLQKNDYSSYTARVEVKDSTAEGYLFSWQVENSILDGLALSKSVEEIAKKY
                     DTLSVLYRTNDAGAFQEIVNWKELRNSLLEMFDALIQEDSAKTPKLKEIMEPVKVMFS
                     TQENITNLVFKEIQLIHYPMGLMFQVKDTIFYKEELSNPLGGDPIQADAKFYFNVIDR
                     KKHLCQFENTLKMDTLDFKRMTVDLFQKMLSNNIFESVTKRDSALNSIKNEMAKLKVD
                     IRDSNVYIYEYNPGWPIKISTQRKSIVYSGDLTLKKVDQLLIEQISQPTPKKEQGLFL
                     GGKKATNQKTKAKKNKK"
     gene            16449..17483
                     /locus_tag="PLT31_01640"
     CDS             16449..17483
                     /locus_tag="PLT31_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HPW93866.1"
                     /translation="MNYIIEKALDNKRISPEEALILLREVDWTEIVHAGNEIRKRRYP
                     QNKVGYTAYRIINYTNICSIQCSFCSFCRSRENPESFVLSLDQIRQKALEAKSLGVNQ
                     IFLQGGVHAGLPLNYYVDTLQMLTQELHVEVRAFSPVELVYIAKAHHMPLTDLLDLLK
                     KAGLSSVPGAGAELLSDSMRQKLSPKKLSAKEWGDTMILCHEKGLPGSANIVFGSDET
                     PKDIIEHLQVIRDIQDKTQGFLTFVAWTFQPQTPHFPIRHVTGQEYLKIIGLCRLFFD
                     NISHIEVSLLGMGTSLGELGLYSGADDINSIVIEENVLKSHGLTTIEEAESFIKNAGF
                     TPYFRRFNFD"
BASE COUNT         5716 a         3540 c         2963 g         5390 t
ORIGIN      
        1 catcttcaaa agtgccgtta gatgcaacgg ccaaagcttg ggcatctaca acagcttatc
       61 gggctaatga aaaactaagt ggtgaagaaa tggcttcttt agtcaatcag ctattgcaga
      121 cagaagagcc tttaatttca ccttatggaa agccgactct acttcgaatc cctattgagg
      181 acattcataa gaaattcaag cgataagcat tttatggact ctccctcaca tgttttatga
      241 agagccatcg cattagaata aaagtcttct ttttctaatt cttctaattc ttttactata
      301 gacaacactt cttttttata aaagaaagaa actagactta gaaattcagg gacaattctt
      361 tttgataata atagatttgg taaagtaaaa aatggggttc gaactaatat agaaccaaga
      421 acataggtaa gcgggtctat ccttgaacat gccaaagcct tcgttcccac aagagacgct
      481 tctaaaatac ccgatccagg agtagcgatt accccctgag ctttagaaaa taaggctgca
      541 cgttctacgc tattttcagg aaccaccatt tgttcaaaat caaaattcca tttttttatt
      601 tctttgagga gacttgttct tgaagccaga aatttaatct gtagatttgg attttttttc
      661 ttcaattgtt ttgcaattct tatgtataaa ggcatgtttc gatgcaattg atggcgccta
      721 cttcctggaa ggaataaaaa gtcactccct ttattcggtt tttccttcat taaagaaaca
      781 gcaccttgta aaaatggatt ttctattttt aatgaagggc aatcaaattt tttataagcc
      841 tctcgttcaa aatcgtataa aacacctaaa gtcacatctt gtagagaata ggctcgtttg
      901 gatttccatg cccatatttg aggaggggca atatataaga taggtttgga atacttttta
      961 gaaagttcca ccaacctcat attaaaacca gggtaatcga taagaacaac agcaatacaa
     1021 ttaggacatt ctaatatttt ttttaataaa acaaaagaac gatgtaattg gaaataatga
     1081 gttaatacat ctttaaatcc acttatggca aaatcatcaa aacacgccat ttgtttcaag
     1141 ccctcttttt gcatgcgagg gccacctaca ccaattgctt caacacccca aaggttcatc
     1201 gcagaaacag cttctgctcc taaaatatca ccagaatctt ctccagcaca aaaaaatacg
     1261 aactttttat tctgattcag atgttccata ttttgcatcg atttattttt aggagctcta
     1321 tttttgatga gtttcataaa tcagcttcga tttttatttg acacccatac atctgttaca
     1381 ccaaaatctt tttttaacca aacaagtaat tcttgttcgt atttaaaacg aatttttttg
     1441 gccactaaag catgctcata tccagaaagg gattcaatat gaaatatcag ttgacaagag
     1501 gattcaccag ggaatggttc atagcttttt aatttttcat ttaagagact aagagtttct
     1561 tccgtgatgg caagtgatga aaaggaaacg tgtaaaaact tgacccattt ttgacgaacc
     1621 gtttctaaag gcacaatttt ttcaacaacc acttgtaggc ccttatcatc tcgttgcatt
     1681 tcaaggacgc cctgcactaa aacacggtca tcaatcgaaa cacgatctcg gaaagcttcc
     1741 catttatcag aagttaaaaa caatgcaatt tcgccttgaa aatcttgaat tatccccgag
     1801 ccaagggtat ctccgcgtct tgtttcaaaa gaacgcattt tagtgatcac gccaccaatg
     1861 gtaatacttt ttttaagtaa tcctgaatta ttaatctcgc tttctgctaa actgcatgta
     1921 gtaaagcctt gtagttctgg gcgatattct tctaaaggat gccccgagat aaacattcca
     1981 aggacatctt tttccttatt taataattcg atataagtcc attcttcagc atcttctaat
     2041 tcttctgctg tatggataca agtgtcatta tctcctaaat caaatataga gacctgacca
     2101 caatctatat tttgacgata acgattagcg acttcaatgg ccttgtcaat cgttgcaagc
     2161 aatgcggaac gacttccagg aaactcatcc aaagcccctg acatcacaag actttctagc
     2221 acttttttac ttaaaggcga ttttttttca tttaaagaag cctggtaatt taataggcgc
     2281 ttacaaagat caaaaatatc cttgtattct ccatgtaacg ttcgctcttc aacaatgtct
     2341 tcgactatgg aaatacccac attttttatg cctgctaatc catataatat tcgaccctgc
     2401 tcatctacag aaaatgttgc aaatgaagaa ttaatgtttg gccctaaata aggtatgtcc
     2461 attttagaac attcttttaa gagactcaca atattttctg tctttgatat ttctgaagtc
     2521 atcgaagcgg ccataaattc agggccataa tttgctttta agtaggccgt ttgatatgcc
     2581 acataagcat aggcagccgc atgactttta ttaaaagcat aaccacagaa gggcaataat
     2641 actttccaaa tttctttaac taaagaagct ttataaccac gctctaagca cttattcata
     2701 aattcaggct ctaatttcgt catttcttca ggcttctttt ttgccataat tcgacgaata
     2761 atatcagcgc caccaagcga atagccgcct aatttttgag cgatacgcat cacctgttct
     2821 tgataaacga tcacgccata agtttcactt agaataggct ctaaatcttc gtgatagcag
     2881 tcaacaggtt cttcgcctcg ttttcgagca atgtaatgag gaatttgttc aattggtcct
     2941 gggcgataaa gagcattcat cgcaattaaa tcttcaatac gttcaggaat caatttccga
     3001 aggtactctc gcattcctgg agattcaaat tgaaaaacgc ctaccgttaa tcctttactt
     3061 aataaatcaa aagttttagc atcatctaaa ggaatacggc caatctctaa atcgacattt
     3121 ctggtctgtt taattaaagc aacagcgtct tgaataatgg ataagtttcg aagacccaaa
     3181 aagtccattt ttaatagacc gatatcttca gcgtaatgct tgtcgtacat gatcacaggc
     3241 gtatcttctt tgcgagctct aaaaagagga gctaaatcag acatggccac tggagcaata
     3301 atcaccgctg ccgcatgaac acctgtttgc cttgagagat tctctagctt ttcggagtaa
     3361 ttccacaaat cccgatagtt ttgccttgat tcaataagtt cgtttaatgg cccaggatta
     3421 tacccttcta aaggagcccc ttttttattt tttcccgttt tggccatctc cagtgttgca
     3481 cctaaatcta atggcaatag ttttgtaatc gctcgcacat cagaaatggg aatacctaaa
     3541 acacggccaa catcattgat aacggctttc atttttaagg cgccataagt tacaatttgc
     3601 gttacgcact ctttaccgta ttgatccgaa acatactcaa tcacacgagc tcgatctcga
     3661 tcggaaaagt ctgtatcaat atcaggcatg cttacacgtt ctggattcaa gaagcgttcg
     3721 aaaagtaagt caaaccgaag aggatctata tctgtaattc cgataatata ggtgacaata
     3781 gaaccagctg cagaaccacg cccaggccca actggaattc cattctcgcg agaccagttt
     3841 ataaaatccc acacaattag aaggtagccc gcgactttca tttctcgaat acaagcaagc
     3901 tctgtttcca tgcgttcttg aacttctggc gttacctctg gatatcgatt ggataccttt
     3961 tgtctacaaa ggtaacctaa atagtcatca gaatctttaa actcatcagg gaacttaaac
     4021 tgcggccaga attggtcgcc acaatcagtt tggatagtaa tatcacattg ctctgcaatt
     4081 ttaacagtgt tttcaatcgc ttcaggatac tctttaaaag cagagaccat ttcttccgtt
     4141 gttttgaagt agaatttatc tgttgggaac aaaggatcac tgaaattctg aagtttttcc
     4201 gcaagagcta tacagcgtaa gaccttgtga gcaggagcat cccctttttc aatgtaatga
     4261 acattattca cagcgacaag cccgcgatga agatcgcgag acgcttgcgc tataaaagca
     4321 ttgacttttt tttgcccttc aatttcttga tcttgtacaa taaagaataa actctctgaa
     4381 tcaaaaatag aacacaaatc cgttaaatat tgatttgcaa tatcttcttt ttgatttaaa
     4441 acatccgagc caaaacggct aaaaaagtct cccgctaaag cgattaagcc ttttgatttt
     4501 tcacgaatga attccaaagg aactgcaggg acttcactcc actgttcagc atcttcatag
     4561 caataactaa caatctttaa gagattccga tagccttcgt ttgtttttgc aattaatgtt
     4621 aaccgctgat acccctgttc ctcttttgtc atcgaagttg aagttgctac ataaatatga
     4681 gagcctaaaa gagctttgat cggaggaagg ccttcttttt gtcggctctt atttaatgaa
     4741 cgagcctgca tataaaattc taaaatacca aacatgttgc catgatcagt caaagctact
     4801 gcaggttgat tatcctttat tgcttctgca agcaattcat gtatacgaca agaagaacgt
     4861 aataacgaga actcagaatg cgtttgaaga tgaacaaaac tcatagatta gctcctaata
     4921 aattttacac cgcataacat tcatactaac gtattccaag gagcgcgtaa agagtttttt
     4981 cttgaatgta ttaaaagaga agatcattta acaaaacctt aattatacca ttctaaatat
     5041 ataatttcaa aacttttttg atagacttgc agaagctaat ctaatcgtaa aaatcttttt
     5101 ttgttttgca aattagccac ttttttattt ttaaatagat ttattttgtt tttgacattt
     5161 tttgtcataa aaaagcattc taaaacatcg atgaaccgct ttatctttat actgatcttc
     5221 ttctgaggtt aattcttcat ttgtataacc actatcaatt gattttagaa gaatttcttt
     5281 tttttcttca ttgtaagaaa ataaaatacg aaatgtccga taagccagaa tggcttcatt
     5341 agcttcgtta ttttttttaa cgactctttt ccgtgtatca tctaaaggat agctacttaa
     5401 ttgtacctga gcaaaagagg ccatattcca agagagttgc tttaatatcc aatcctgctg
     5461 ttcttgaaat aaaggtgtcg attcaatttt ccacaatacg gattcttgta aatccaccca
     5521 acccgctttg gcatcaggga acgaatctac ttttggaatg tagggtttta tatctagtat
     5581 gggagtatca tctagcaaat cacattcatc tacaaaaaga tgtaagccat tgattttcaa
     5641 taaacggaca caacttagcc ctattgcatt tggacgatat ggacttctcg aagcaaaaag
     5701 gcctacacga tctcgatcta taggaggaat aggaggcttt gctgtaggtc tccagccttg
     5761 attttgatga aattgaaaaa tcaaccaaat tcgctcaaaa ccatctaaat cccgcaatgc
     5821 ttgttcgaaa tcaaagccag attttaatgt aatgatccca ggatgaccct gaaaaaaaac
     5881 accctgacgc gctacatcat atttataaga aaaatctgta tgaaaaacac ctatggaatc
     5941 acattgcatg ttttaaagtt aaaatttctt ttttctaaat taggcggtga tgaaagataa
     6001 agtcctaaaa ctaatacaaa aatacgaaga attagaatct gaattaagtc gtccagaagt
     6061 cattgcagac caaaccaatt atactcgtct gcgtaaaaca tttaaaggca ttgaaaaagc
     6121 gactaccaaa gggcgcgaat ttattcaaat gtctaatgat cttacggaat ggcaattagc
     6181 tctgaacgat cctgatccag aactgaatca atgtgcaaaa gaagaagtaa aaagtctaga
     6241 aagcaaaatt gccgagctta cagaagagct tcaaatatta atggttccta aagagccttg
     6301 ggattataga aatgccaccc ttgaaataag aggtggtact ggaggcgatg aatcctcttt
     6361 atttgcaggc gatttattta gaatgtatcg aggctattgc gatcgaatgg gttggagaat
     6421 gagcatttta aatgcttctg aaggcaccgt tggtgggttc aaagaaattt ctgcttttat
     6481 tgaaggcgat tccgtctatg gtactctcaa gtttgaaagc ggagtgcatc gcgtgcaacg
     6541 tattccagac acagaaaccc aaggacgcgt tcatacttct gcagctacag tagctattct
     6601 tcctgaagcc gatgaagtcg atatggaaat tcgagaatcg gatattcata tggatactta
     6661 tcgtgcaagc ggcgcaggcg gccagcatat taacaaaaca gattctgctg taagattaac
     6721 tcatattcca acaggtgttg ttgtgagttg tcaagaagag cgaagccagt taaaaaacag
     6781 aagcaaagcc atgcatatgc ttcgttctaa acttttagat gctgctattt caaaaaaaga
     6841 acaagaagaa gccgctagtc gaaaagcact tgtaggcact ggcgatcgtt ctgctaaaat
     6901 aagaacttat aactacccac aaaatcgatt gacagaccac cgtattaatt taactctata
     6961 cagtttagaa tctgtgatga ctggaaatat tcaagaaatt atcgacgctc tacaaatggc
     7021 taatgctcaa gaaaaattag gcaagtttag cgtatgagta ctgttcttga aattttaaac
     7081 gtcactacag aattctttaa gaaaaataaa gtccctgatg cgcgcctaga tgctcaatat
     7141 attttagctc atggcctaaa aatgaaacgc atggacttat acctaaattt cgatcgtcca
     7201 ctcactgaaa atgagcttac gattttaaga cctctagtcg ccagaagagc gaaaagagaa
     7261 ccgctacaac atattattgg aaacacgtca tttcgtggat ttttaattaa ttgtgataaa
     7321 agggctttaa ttcctaggcc agaaacagaa atgatggtag attttgtcaa agaggaaact
     7381 tctttgccag aagagcctct gattgccgat atcggcacag gaactggcgc cattgccatt
     7441 gctcttgcaa aggaaattcc ttcagctaaa atttttgctg tcgatatttc gaaagatgct
     7501 ctagatcttg caataagcaa cgccactcaa aacgaagtag aagataaaat ttcgtttttt
     7561 gaaggagatc ttctttccgc tcttcctgca gaatccaagc ttcatgtgat tgtggcaaat
     7621 ctcccctata ttccacttaa agatcaagca tcccttcaag aagaagttcg tctctttgat
     7681 cccgctttag ctttatttgg cggagaagat gggttagatc tcattcgtag acttctcaga
     7741 gaaactgaaa atagacttgc gctaggagca gtcatttggc ttgaaatcgc ttctgagcaa
     7801 gaagaaattc taaaaaatga gatttctcaa tacccttggc tttcttatac ccgagctatc
     7861 cctgatttta tgggaaacat tcgttttatt gaacttcgaa acaagaatta aaaaacgtta
     7921 ctcaaaatca tacatgctat tgtatgtgtt ttcgagtatt tcttcttctt ttgtttttac
     7981 ttcttctgta ggtgacgttg ttttcttcgc ttgtaatgat tcgtaatact gttttgacaa
     8041 acgttcaata tgttcttcat tttgtttaat acgctgacgt aatcttttta gttcatcgtc
     8101 agtaatagtc actctagttt gtaagcgccg atcggcctct ttaccccaag ccgtttggcc
     8161 atatttagat cttaattccc taaatacaaa tacagcacta tcttttgtat taggattcat
     8221 ttcataaaaa attcccttca taaatagggc ttgtatacct gattgcgttt ctggatagtc
     8281 ttgataaaga gcctcatatg ctgtaaatgc ttgtcgataa gcagaatcaa cttcggccat
     8341 tttctcaaga ggaatatttt cagccgctaa ccaaagactt tctgctttta ggaagcgctc
     8401 ataagcaagg tcttcatttg ttttgacagt aactctcatg cctaaattca cttgagcctg
     8461 cttagcatat tctgtattag ggtacttttc aattatttct ttataaagaa tttcagcttg
     8521 atcggggtcg tttttaaatt catcataaat aaacgcacga gcataagaag cacgtaacac
     8581 ttgaatacta tcgtcagcct ttacaataat atcatttaag cgttgaatag cactatctac
     8641 ttcggatagc ttaaataaaa acaattctgc aatctgaaac tcactatcaa aaaatctctt
     8701 ttgagtagtt tcaacagtat ctgtttttat tttttcattt gcattttgta aagcaagcat
     8761 tcgtcttaac gcatctcgtc gttctcgact atcgcgtccc caagtactac ttggtcttga
     8821 tacaaaacta ctgtcataat agataagagc ttgttcataa gcacgcttac gcacttgctc
     8881 atcatcccct aaatggaaat aacttctcgc agtataatcg gttcttgggt aatcatgtat
     8941 tgccttacga aaagcactac gcgcctcatc aattttatca cccagtttca ataattcacc
     9001 acaacgaaca atgaattcag gcaggctttc ttcataatct ttttccacca ttagcttttt
     9061 atattcttct gctgcaggca gatactcttt ttgattgact aaacactcag ccgcttgaat
     9121 agaggccgtc attttttctc taaccgttag ttctttaatt tctttcgcta aataatgttc
     9181 tcttgctttt gcccaatttt caatcttaaa atataagcca gctaatcgaa agtgtatttt
     9241 cgccctcata taaggagtgc caagcgtatc tgcaagcatg ttttcgagcg ctgcaatagc
     9301 ttcattgggc atgtctgatt tttcttgaag taaagacaat agattttgtc cttcgtaata
     9361 ataaggatgt gatttgcctg cttctaaaac aggttccaaa gcgaaacggc ttatattgta
     9421 ttccccatta cgataaagac aataagcccg ctgatactga atcacaggca tagaatcgtg
     9481 atctgcgaaa tagcgttcaa actcatcata ttttgtgatt gccttattcc actcttgctt
     9541 atgacgaaaa gactctccca ttaaaaaaac agcttcggca gtccgttttt tatttttagg
     9601 aaaacgttct aaaacacgag accccttttc tattactcga tcgtatcttt ttcgttcttc
     9661 catcgttggt agagaagaat ctcctggaat actatcaagt ctagcggttc ttaaatcaga
     9721 agcttcctcg aatgcgcgtt ccgcattaaa catgtgattt aaataagcac aacatgtaca
     9781 gccttgaaaa aagactgcca tgaaaaggct aactaaaatc cataagtaat gatgtaaaaa
     9841 agaacgcata acaggaaaat acaaaaaaga agaccttaat atttattcaa ataagacaaa
     9901 taatcacaaa gtttcatttt atcagaaatc tgttttttat ctaggcgcat cattctaact
     9961 tctgaaggtt ttgaaacata gcgtgcaaga atttcaaaat tatcaagagt ttcccaaaca
    10021 gcagcatcta taacagtccc attacaatcc gtttctccag tgcctattcc tatgcctgaa
    10081 atacgtgaat tttgtttcaa aagcctatta tagatatctg gagcagagcc taaatgattg
    10141 gaattaccag aagaatcaat aactaaaacg tgcacgccat agaattgatt tgttttacta
    10201 aatagaactg tatactgatg ataataagga ggttcataga aagattcttg ccaagtattt
    10261 tttttaattt ctttaaaatt agtcacagca tactgagtga aaaattcttt tggcgaagca
    10321 ccatagcgaa ctaaataatg acctagcatg gtgctaaagt catgcttact agaaggcatc
    10381 gtggtgcgaa tactatccat cactcgattt aagctttccg ttaaagcacc atcattttct
    10441 gaacgtaaag aggtcacaga ggcttcttgt aaacgcagtt gaatttcagg gtattcagga
    10501 tcttttgttt gaatttcctt aaactgttgt tttgccttat caaaagagcg ccctttcaaa
    10561 taagctaagc caagtgcttc ccgtagcggt aaattctctg gataagtcgc aactaatggt
    10621 tctaaaatat cataagctaa ttgagcttta tctctggggg taattcgagc actagaggaa
    10681 tgagctggag gagatttttc tcctaaagta tttaatgccg cttgtgcttc cttataattt
    10741 ggatttaaag ctagaattcg ctgatagttt ttttcagcca tatcaaaaga tccgttatac
    10801 tcgcttaaat aaccttgtag aagtaaaagc ttcgtgtctg caggatactt ggttaatgca
    10861 tactcaatca cagaggtaca actatctaaa ttgccctgtt gatgataaag taaggcgaga
    10921 ttttcatatg ctttttgaga agtccctttg gttaaaccaa tcgccactcg cgtttcaccg
    10981 atcgcttgcg ataatctatt gacctcttgt aagagttcac cataccacaa acggaaatca
    11041 ggaaaagagg ctcctttttc agtggccttt cgagcaagat ccagagctag aggtaaactc
    11101 cctaaacgat aagcttcacg actatcaatg tataaatcaa gagaagcgct agttgctttt
    11161 ttcttcacgc tgcgaatcaa atgttcgagt cgcaatagct catttgagga aagagaatcc
    11221 gactcccata agacatgtag gacaccccac tccaccatga aaagttctgg ataagaaatt
    11281 aaaatagcat caaagatttc tatggcttgt tttgatccac cttgtagcat taaatcaaaa
    11341 gctcgacgca cttcatcacg agtagaaaaa ggcaattgat caaaaccaac ctctaggtta
    11401 ggaacatctc ccctgaccaa aaccgtacct gctggaggcc caaaagaaac gccctctaaa
    11461 acaatctttg atggtccaaa tttttgatag ccgaaataaa ggaaataagc aagtaaaacg
    11521 ccacccgtta aggtcaagaa aaatttagcc gcggtacgag cctgagatga agaaactcgc
    11581 attagctctc caaaaagtca gccagctttt ctttattctc tttacttttc tgaagttttc
    11641 tcaatgtttt atttttgatc tgtcgtacgc gttcccgact taattttaag tcttcaccaa
    11701 tatcttttaa tgtggtgtct tcaatagaat ctaagccata aaacattcgc aatatttctt
    11761 cttcacgttg aggaagagaa gaaagagttt cctgaattag tttgcggcgt tcttcgcgtt
    11821 caagttcgtc ttcttgattt ccctctgagc caagaacatc cattaaagta ctgacagaat
    11881 cgcctccaga ggagctatca tcgccaatag gagcatcaag agagatatca actatttttt
    11941 ccatgacttc cacaatatcg cgttcgcagg atgcaaattc ttctagagca atagtggatt
    12001 cataatcgcc cccattttga acaagagctc ttttaaaacg attgactagg gctaatttat
    12061 taggaggaat tcgaacggca cctacttgtt caaaaagagc tttttgaatg gattgacgaa
    12121 tccaccatac agcgtatgaa atgaacttaa cgtctttttc catatcaaaa cgcttagctg
    12181 ctttaaaaag gcccaaattc ccttcgttaa ttaaatcaag taaagaaaga ccacggcctt
    12241 gatatttcct tgcaacactc actacaaaac gaagattacc tcgaatcaaa cgctgcaaag
    12301 cagagttacg aatctcttca gattcatttt ttttgatgat cgtgagtaaa acagcttcct
    12361 cttgaggtgt aagagttgga tatctattta agtcacgaaa ataaagagat tcgttaaagt
    12421 cactcatata taaagcacct atacgttcca aacgctccaa aaatttatga agtcaaccca
    12481 aatatggttt tttacttcgt tttcgtcaat tccttaagtt gtcggtaagg gtaaattcca
    12541 ctacgatgac catctgtcca tgtaattcca acagcataac gacctattga gcgcaaattt
    12601 attatcgaaa tatcttcagg gatcgtattt ggatctaata ttcgttctcc tgtatgctcg
    12661 tccacacata aagcacaagg acaagctact cgaagagccc ttaaattaaa aacagaaaaa
    12721 gacccatcat tccaatcaaa acgcacttca ttttttggag tcttagaaat tttcacaggc
    12781 tgtaaaatca taaatcactc cactcataat tataattcga aagcacttgt tcattgtttg
    12841 tagataatac agataaagca caaaccactt gttcagcaat tcgaataaat tctgttgcag
    12901 aaacactatt tggatgagca agaacagctg gagcacctaa atcgcccgca tcagcaacag
    12961 aaggttctaa tgggattttt ccaagtaaag gaacaccata ctgtttcgtg attttttctc
    13021 cgccaccagt tctaaaaata taatgggatt tttcacattg atcacaaata aaataactca
    13081 tgttttcaac aacacccacg acagccactc caacagcatc aaacatcgca agacctttac
    13141 gacagtccgc taaggctact tcttgaggag tcgttactac aacggctcca gacaaaggac
    13201 agctttgcgt taaggttaat tgaatatccc ctgttccagg aggaaagtcg atgagtaaat
    13261 aatctaatcc accccaccta actcggtgta aaaaattctg aatcatctga ctggccatag
    13321 gaccgcgcca aatagttgct ttatcagaat cagcaaacat actcatagag acaatggaaa
    13381 tgcctcccct agcaagtaca ggagaaatag ttttatcagg atgtacttga ggcgcttctt
    13441 caatcccaaa catcacaccc atagaagggc cataaatatc agcatctaaa ataccaacac
    13501 gagccccaga taaacttaaa gccatcgcca aatttgcagt gaccgtagat ttacccactc
    13561 cccctttacc agaagaaaca gcgagaatat gagaaacatc ttttaaaaat gtattttcgg
    13621 aaaggttgac ttgtgatggt tgtgttttcc ctgatagagc aatagataca gactttgcac
    13681 ctaaatcact caatattttt ttacaatcca caacaaattt ttcttttaga ggacaagctg
    13741 gtgttgttaa aaccaaatca aaagaaacat catcgttctc tgaaatcaga atattttgaa
    13801 caaaacctag ttctacaata tttcgatgta gatctggatc tattatcact tctaaagctt
    13861 ttaatacttg tttttcattc atatttacaa attagtaaaa cgttccattc tttttataaa
    13921 aaaagtgaat tcaactccat gaacaaacaa aaattttaca acggcattga gaacttaggc
    13981 atatgcaata tatgagtcat gcattttggc cattcgttaa gataatgcga tactaaaata
    14041 attgttgtag atgaagacag ataatcaagc caattaaaaa tctttgcacg atgtttagca
    14101 tccatccctt gagttggctc atctaaaatc aataccgaag gaggttttat cgccgctctt
    14161 gctattaata ctaggcgttt ttcaatgagc gttaatgaag aaaatgcaac cttcgtatct
    14221 tcaaaaccta aaaaatggag ccactcatta gccttttctc gttcttcaaa aacaggttct
    14281 ttaaacaagc ctaaagaagt agaaaagcca gtacaaagaa cttcttgaag agttaattcc
    14341 tctcggtatt gcaaagccaa ttcaggagag aaaaagccca acttttcttt atgagcccaa
    14401 acatctaatc cttttcctgg acgttctcct aaaagcataa tgtcattttt ataaatctgc
    14461 ggatgatctg cagaaagaag ggcaagaagt gtacttttcc cagcgccatt aggccccatc
    14521 accacccaat gttctcccga gcgaacttgc caactcaagt tttggatcac ctctgtttct
    14581 ccaaaacaaa cattcacgtt tttcaaatca aaaaggagtt ttccttcaac tgcagatgaa
    14641 gtaagctttt caatttcata ttcagaatac gattttttta cttgctctga tttaagagcg
    14701 ccttctcttt tcttttccaa taaaacaaat ttcccattct cataaacaaa agcaggaatt
    14761 aaaggcataa cttcttcttc tcttgcaaga cgaatgacca tttttacatc agaatgaacc
    14821 atctttaaaa cgcattcttc caaataaggc cggtaagcgg catctagccc gccaaacaaa
    14881 tcgttaaaat aaacccattc tggattttcc atccaaactc gagctaataa aattcgtctt
    14941 aattctccat tggaaagact tagaagagtg cgatctaaca cgctctctgg caaagaagcc
    15001 ttttgcaaag cctctgacaa aagagctgga tttgttttag gaaaaggcgt atcttcaacc
    15061 actaaatccg tctgcaaaaa aaacaacgga gaaagtaaaa aatgacgaac taaaggggct
    15121 tctttatttt caatacttat aaaacgctgt tgccaaaagg gagctaaggc tctttggatt
    15181 ttacgctgaa tacgttcaga aacaactgat agacgttctt gtttttttaa aaaggaccaa
    15241 agaatgggat ctacagggtt gaatggaact tctaaaatct gtacttgttt atactctttt
    15301 aataaagatt caatatcatc aaaaatcatc ttataatgat aaaaatattc tctttaatag
    15361 gctatattta attggactct tttacaaaac cagtttatga tcaaaaaaat aagccctttc
    15421 ctcttgttga tttgcgctat ctctgccata gcacaaacgg attctaccct atcttttata
    15481 gcttattgga aaaagggaga cataaaatat ttcaatattt cccaaaataa gtctcagcat
    15541 aaaaacgact ctcttcaaaa aaatgattat tcctcatata ccgcacgagt agaagtaaaa
    15601 gactctactg cagaaggcta cctttttagt tggcaagtag aaaactccat tttagatggc
    15661 ttagcacttt ctaaatcagt agaagaaatc gccaaaaaat acgatacttt aagcgttcta
    15721 tatcgtacaa atgatgctgg tgcttttcaa gaaatcgtaa actggaaaga gcttcgtaat
    15781 tctttattgg aaatgtttga cgctctgatt caagaagatt cggcaaaaac accaaaacta
    15841 aaagaaataa tggagcccgt aaaagtcatg ttttctacac aagaaaatat cactaatttg
    15901 gtattcaaag aaattcaact cattcattat cctatgggat taatgtttca agtgaaagac
    15961 actattttct ataaagaaga attatctaat ccacttggtg gagaccccat tcaagcagac
    16021 gccaagtttt attttaatgt cattgatcga aaaaaacact tatgtcaatt tgaaaacact
    16081 ttaaaaatgg acactcttga tttcaaaaga atgactgtcg atctttttca gaaaatgctt
    16141 tctaacaata tttttgagtc cgtaacaaaa agagattctg cactgaatag cataaaaaac
    16201 gaaatggcta agcttaaagt agatattcgt gatagtaatg tatatatcta tgaatacaat
    16261 cctggatggc cgatcaaaat ttctactcaa agaaaatcaa tcgtctattc tggtgacctc
    16321 acactaaaaa aagtagatca attattaatc gaacaaataa gtcagccaac accgaaaaaa
    16381 gaacaaggtc tctttctagg tgggaaaaaa gcaacaaatc aaaaaacgaa agctaagaaa
    16441 aataaaaaat gaattacatc attgaaaaag ccttagacaa caaacgcatt tctccagaag
    16501 aagctctcat tcttttaagg gaagtcgatt ggacagaaat agttcatgct ggtaatgaaa
    16561 tacgaaaaag acgataccct caaaacaaag tgggttatac agcatatcga attattaatt
    16621 atacaaatat ttgttctatt caatgttcct tttgttcttt ttgtcggtca cgcgaaaatc
    16681 cagaatcatt cgttctctca cttgatcaga ttcgtcaaaa agcacttgaa gcaaagtctc
    16741 taggagtaaa tcagattttt ttgcaaggag gcgttcatgc gggccttcct ctaaattatt
    16801 acgtggatac acttcaaatg cttacacaag agctccatgt agaagtgcgt gctttttctc
    16861 cagtggaact tgtttatata gcgaaagcgc atcatatgcc tctcactgac ctattagacc
    16921 ttctaaaaaa agctggttta tcttctgtgc caggcgcagg agcagagcta ttatcagatt
    16981 ctatgcgtca aaaattaagc cccaaaaagc tgtccgcaaa agaatggggc gatacgatga
    17041 ttctctgtca tgaaaaaggc ctcccaggaa gcgccaatat tgtgtttgga agtgatgaaa
    17101 ctcctaaaga tatcatagaa cacttgcaag tcattcgcga tattcaagac aagacacaag
    17161 gctttttaac ttttgtggca tggacttttc aacctcaaac ccctcatttt cctattcgtc
    17221 atgttacagg ccaagagtac ctaaaaataa ttgggttatg ccgtctattt tttgataata
    17281 tttctcatat tgaagtttct ttactcggca tggggacatc attaggagaa ctagggctat
    17341 atagtggcgc tgatgacata aatagcattg tcattgaaga aaatgtttta aaatcacacg
    17401 gattaacaac aattgaagaa gcagagtcat tcataaaaaa cgctggcttc acgccctatt
    17461 ttcgacgatt taattttgac tgagaaaagt ccatctagac tcaactgagt ttttttatgc
    17521 cgaggcaatg aaaatctcat ctaaccaaac ccagtttttt tcatgcagag acaagcgtat
    17581 ttttgtacca caagggtatc actctcaca
//
LOCUS       DAOOBB010000017        17579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_4223_length_17579_cov_4.434319, whole genome shotgun sequence.
ACCESSION   DAOOBB010000017 DAOOBB010000000
VERSION     DAOOBB010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 17579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17579
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4223_length_17579_cov_4.434319"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1488)
                     /locus_tag="PLT31_01645"
     CDS             complement(<1..1488)
                     /locus_tag="PLT31_01645"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HPW93867.1"
                     /translation="MNPWSIFALISICILFLGYQSGVVFLAIAAFLFATWIFSRQRSG
                     RVAQLHKGILYLAIIPFSFWWFLSPSIEGQISPWLFFIPAWFCLFLALMQWRSLGRGG
                     ELVFVRFNALAAFLFSIRNPDRYSVVIAVLFIAVFLWHIRPRLSLAKWLISLTSVILI
                     SFLTLFISTTVKANQTQKRSGNWGEDYYLSRHIMGFDPVASLGSFEKNYDSKYDDQIV
                     LRLWTSTPPIYFKAVAYERFFQGLWKVSNHYDVFYPERYEVDYALFELESKTENPVEK
                     VWVQSALNTFHYLFAPSNATGVSVKAADSVYYYRGGVWHSPGIERSDWYYFKNSVSLD
                     TAFTSSFLSIEKRDLALVRKASLEMGLDTLLSVSEKVKRIRSYFATHFRYSLYLPIKK
                     KNNALQVFWDTKSGYCEYYATLATLLLRDQKIPARYVVGFAFPEFAENGKYSLFRRRH
                     SHAWVEYFDSSWKITDPTPIVAQSDEIEGYWTRLQEKIKAQTAYVM"
     gene            complement(1485..2666)
                     /locus_tag="PLT31_01650"
     CDS             complement(1485..2666)
                     /locus_tag="PLT31_01650"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPW93868.1"
                     /translation="MRLLEKLRAYQKAIPETPYREGLLIRLYYFWFERFTSAGRALIS
                     AAWIALLLFMVPGAYLARFFLVLLFALLLTSFIIRERKPKVKIISVSILPVREKETGV
                     LVASIQALDDYPSVSIDAFRASPSLKKMTCVSRFFKTNEVESVELKIKTAHRGSYIFK
                     NIAALVPDPLGLIQTPVFYNGSLEFLVYPKRLKISQFSFLTKGHPGREFALLLMPSLQ
                     RGVDFSGVRPYREGDSVRDIDHRAYARYLKPFTKEFSVEEGSSVVFVLDIRFSSFSKK
                     IFMEKAIALAAAMATWFEERQILGRFFINNQEIDLTRGEKLDLVMAALSRIETPRFKS
                     SEPPGIWSPEALPMGPVFAITLSPNASNLIQKQIVVSDETGGDDSLMYFDPSNEEEVF
                     L"
     gene            complement(2669..3577)
                     /locus_tag="PLT31_01655"
     CDS             complement(2669..3577)
                     /locus_tag="PLT31_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPW93869.1"
                     /translation="MLKQLINSLNHVLLGKEKEIRLLLNALLADGHVLIEDVPGTGKT
                     TLAKALAVLVGADFSRIQFTPDLLPGDVTGGAIYRQNTGTFEVHRGPIFTQILLADEI
                     NRASPRTQSSLLEAMEERQVSLEGSRHELPALFMVLATQNPVEFHGVFPLPEAQMDRF
                     IIRLTIGYPDEETELNILRSQRFTRPLDSLQPVITPDQILILREKVKKTHVEEALERY
                     IISIVLATRSHAGLRLAASPRAGLAVLRMAQANACLDERDYVTPEDIQFVLKSVMAHR
                     IFAIESGFGVTEGILQEIVKNIPIPR"
     assembly_gap    3874..3883
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3884..4505
                     /gene="grpE"
                     /locus_tag="PLT31_01660"
     CDS             <3884..4505
                     /gene="grpE"
                     /locus_tag="PLT31_01660"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPW93870.1"
                     /translation="ADQETSKNINPEEDDLIREAEACACANEEACTDKTSADPDVKEE
                     ENKEKESSENPASEIDVLKAALSASNDRYLRLMAEFDNYRRRTAKEQLEIIETANAKL
                     LEQLSEVLDNFERASLPENRGCDIEAFAKGMSMIHDQFEKTLRDAGLEQINPVGELFD
                     PSCQEALIKQPSEDIEENHVIQVFQKGYRLKHKIIKTAKVIVSAGK"
     gene            4645..6573
                     /gene="dnaK"
                     /locus_tag="PLT31_01665"
     CDS             4645..6573
                     /gene="dnaK"
                     /locus_tag="PLT31_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPW93871.1"
                     /translation="MSKIIGIDLGTTNSCVSVMEGGKPVVIANAEGFRTTPSIVAFAK
                     NNETLVGHVAKRQAITNPEKTIYSIKRFMGRTAGECSDSEKNMPYHLVGTGSSPVRVK
                     IDDKEYAPPEISAMILQAMKKIAEDYLGQSITQAVITVPAYFNDAQRQATKDAGKIAG
                     LEVLRIVNEPTAAALAYGLDSKKSEKVAVYDLGGGTFDISILEIDEGMFTVKATNGDT
                     MLGGDNFDETIINWINDEFKKSNPGIDLKSDKMALQRLKDAAEKAKIDLSATTSTSIN
                     LPFITADATGPKHLDLTLSRAKFDQLTASLVERSIEPCRKCLADSGLTLGQIDEVILV
                     GGSTRIPAVQEAVKKFFNKEPHKGVNPDEVVAVGAAVQGAVLSGDSAVKDVLLLDVTP
                     LSLGIETLGGVMTKLIERNTTVPTKKSQVFSTAEDNQPAVTIHVLQGEREFARDNRTL
                     GRFDLQGIPAKPRGVPQIEVTFDIDANGIVHVSAKDKETGKEQSIKITSSSGLSEDEI
                     NRMVKEAEMNSQKDKEARELVDIKNQAEQMAYQAESQIKEAGDKIPADTKSQLEAAIQ
                     EIKDKKENGTKEEIKAAMDKLQNMLGSMAQAAGAGAQPNPGAGDCGGAGCNAEPESDK
                     KKDGPIDAEYEVVDDDKK"
     gene            6694..7833
                     /gene="dnaJ"
                     /locus_tag="PLT31_01670"
     CDS             6694..7833
                     /gene="dnaJ"
                     /locus_tag="PLT31_01670"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HPW93872.1"
                     /translation="MATKRDYYEVLGVSKDASAEELKRAYKKLAIKYHPDKNPGDKEA
                     EEKFKEAAEAYDVLSDANKRSQYDRFGHDMPNAGGFGGGQGFSSFEDIFSQFGDIFGG
                     FGFGSSGRGGRSAKKAGPPRGQDLQIKVALSYKEILEGVTKKVRIKRYAPCTYCNGKG
                     GSDVTTCSTCNGTGRVRRVTQSFFQMVSESACPTCNGMGEVIKNPCSHCRGEGRVLEE
                     EMISIKIPVGVAEGQYLNMRGEGNCGPRGGASGDLLVVITEKKDDFYTREGDDLHCDI
                     QVPIHRLVLGGSQRIPTLEGGELSIKISSGTQVGSILRLREQGLYPLNGRGGRGNLFV
                     TIQADIPKDLSSKEKQLYQELAEIRKKKEDEQEESFIQKMKKMFS"
     gene            complement(8400..>9152)
                     /locus_tag="PLT31_01675"
     CDS             complement(8400..>9152)
                     /locus_tag="PLT31_01675"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HPW93873.1"
                     /translation="FASDAPGILNSWWGSIAYMFQIYFDFSAYSDMAVGLGLMIGFHF
                     PRNFNGPYRSVSITEFWTRWHISLTTWLRDYLYIALGGNRVSPRRLYFNLFVVMFLSG
                     FWHGANWTFIAWGLYHAFFMIVERYNGKQAIYKKVPFVFQVLITQVIVLFGWVLFRAN
                     TIGEAISMWKSLLGLMPVSAADPLLTAQIFTPLNCFFMIIALFYAISPWRAFEWCQKL
                     NVRRISVALFLFVFACLVLFTQSYNPFLYFQF"
     assembly_gap    9153..9252
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9253..9832)
                     /locus_tag="PLT31_01680"
     CDS             complement(<9253..9832)
                     /locus_tag="PLT31_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HPW93874.1"
                     /translation="MEPWLIVLMFSSTTIDYIAGRFISAEGASQRQRNIALTASIVAN
                     LSTLGFFKYYMFFMGGLNAILSKFGIDTFSVWNVILPVGISFYTFQSMSYTIDVWRGV
                     APPAKNLASFSCYVALFPQLVAGPIVRYNTVAEELASRKHSMDLFLKGITFFCIGFAK
                     KILIANQVGIIADRVFASDAPGILNSWWGSIAY"
     gene            complement(9977..10375)
                     /locus_tag="PLT31_01685"
     CDS             complement(9977..10375)
                     /locus_tag="PLT31_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93875.1"
                     /translation="MKKILFLLLMSFVLAYSADPIVVDARLTEIPGKIPGNDLYNYVY
                     VFKYKVLNVVKGSLKEKEVLVGVYNPKIPRGQVKDKMAANSKGNVREFKAGAKHRLVL
                     VPLESVWQEALEDEYFDDESIRYFAIEVTE"
     gene            10604..11770
                     /locus_tag="PLT31_01690"
     CDS             10604..11770
                     /locus_tag="PLT31_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPW93876.1"
                     /translation="MEKLWNKPFILICISNFLMFFSFYSLLPILPLYIFEKLNAGSTV
                     SGIILASYTIGALLCRPFAGYLVDSFSRKHLYLFTYILFSFLFLGYLVTFTLVLITAT
                     RLIHGLSFGIVTTASNTIAVDVLPSSKRGAGIGYFGVTTNVAFALGPMTGMLLYESLG
                     FNAVFIFSFLTCLIGIVLIYFLKVPMKPIKKDASPISLDRFFLVKATPQFINLILIGY
                     AYGPITNYLALYAKEIGIEKGIGYFYAWLAIGLVSSRLISSRWIDRGYLTTLIKIGLV
                     ILTFTYGSFFWIHSPFLFFLLAFILGIGHGLVGPGYQTMVVNMAPHCKRGTASSTYLS
                     AWDLGIGIGILLGAPIATHIQYSGVFLLGALSVFIALILFVTISTPHYQKNRLE"
     gene            11775..13814
                     /locus_tag="PLT31_01695"
     CDS             11775..13814
                     /locus_tag="PLT31_01695"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HPW93877.1"
                     /translation="MDLTQIPGLGPKRIQKLNQAGFYSIQDLLYHIPRTWLDQTKIHS
                     IKELKAQDKAIVIGHIVRAGIIRRRSSFFQAVLADDSGQITLMFFKNPSYWSKKIMPG
                     MRFVAIGTVSEYRGLQLVHPELQPMEDDADFKGGIVPVYSIPEVCREARMEQRFFIKL
                     YKYLFTLPHLSVSKACPDELCTFLGLVPTIENLKRLHQPKSFGEAMQGRRQLKILELL
                     PFCLRMASRRRHLSLRGSERAIDQGLVMAAEASLPYELTNDQKKALAQILEGLHGKKQ
                     FHALLQGDVGSGKTVVALLAMLSVCGAQEQCALMVPTDILARQHYAFLKPYFEKAELS
                     ISLLVGATSAAERKTILGELQMGLSHAVVGTHALFSKDVFFNKLGFVIIDEQHRFGVN
                     QREALLAKGNYPDLLVMSATPIPRSLAMTFYGDLQTILIKEKPPGRKPVKTRLITPEK
                     RDDMKNFIFQETLKGNHCYWVVNRVENDEEGKSLSVHDIFEELHSWNKDWKVEVIHGQ
                     MDEDLRNQILKDFNAGLIHVLIATTVIEVGVNVPSANLMVIDHPERLGLSQLHQLRGR
                     VGRGGDQAWCFLMCDSNNPAIERLNGFASTEDGFEIAEMDLRDRGAGNLEGNEQSGSW
                     LFRFFDWIEDQNLIQKILEWAEQILDNQNQFDKVVIEKIQQWYSELPQGNEDGIH"
     gene            complement(13828..15330)
                     /locus_tag="PLT31_01700"
     CDS             complement(13828..15330)
                     /locus_tag="PLT31_01700"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HPW93878.1"
                     /translation="MQYLKISNICFILFIFSMVAFAAIPTIKITTQNGSDVTEKNYYV
                     MNMELSDSDNPVHNLSKTGNVDSIKVRGNSTSDASKKPYRIKFDKKTSLFELPAAKSW
                     VLLANYYDNTQILNALAFELGKRFGLDFTPDYRFVDVYINNSYKGLYLLCDQIQVNEN
                     RINISKKDGWLVEFDYHAPEADEIRFQWKWNGLNGEDSLGTFVKSPDYDDPKSANIDW
                     IKRDLDELSTKMFASDFPENGYRDLVDLDAMAKYIMIQKFLDNFDFNSQAQPGFLPGS
                     NFHYKDVGGKITAGPLWDFDLSAGITFSFSPEHFTTTGDDNHHNMIPTHSYYKRFFDD
                     PVFLAKWKKLWDAHQTDINNLGVFIDSIATYLEPSIMNNFNVLSSGASSGGGGFGGFG
                     GFGGFGATYPSSYDSYKTDHVNKLKTWLTERQTFFNTKIDAMNIDISKDIMTFVPFNS
                     KLELQNTSYDLPLYFSVKGHRLGHVKPVHSGIYYVKKGRTFEKITVLGLE"
     gene            complement(15498..16754)
                     /gene="nhaD"
                     /locus_tag="PLT31_01705"
     CDS             complement(15498..16754)
                     /gene="nhaD"
                     /locus_tag="PLT31_01705"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HPW93879.1"
                     /translation="MFLFPILFVVGYVLIALEHPIRLNKSATAMFLGGILWLLFFLFS
                     NNPVEAGSQLTESLGSVASVLFFLMGAMSIVVVVDSYDGFSLITNRIKTNNVRVLLWQ
                     ITFITFFMSAVLDNMTSSIIMVTLVRRILLKAKDRKFFAGMIIIAANAGGVFSPIGDV
                     TTTMLWIKGLISGAGIMKSTFLPSVFAVLIPLLMMTFLLKGTIPVREKNSDSETISTE
                     RMDQYVVFFFGMFGLILVPVLKALLDIPPYMGMLFSLGLLWLVTDLLLRKKDNDFKTK
                     YSISSILKKVDMSSILFFAGILFAVSTLECTGQLKMFADFLQDTLGNIHLVTFSLGLL
                     SAIVDNVPLVAACMGMYDFPMDATPWSLIAYCAGTGGSLLIIGSAAGVATMGIEKISF
                     GWYLRWITPFAVFGYIAGMGIILLIN"
     gene            complement(16830..17120)
                     /locus_tag="PLT31_01710"
     CDS             complement(16830..17120)
                     /locus_tag="PLT31_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93880.1"
                     /translation="MKIRFTIFEIIIGAIAMITGLVLTLLVWDEGYFVGALMLVVTGF
                     LSVLLAIKEIKNPGNKIHTLNVFFTILRRTFLFLNLMLSSLVIGMLTRLISF"
     gene            complement(17132..>17579)
                     /locus_tag="PLT31_01715"
     CDS             complement(17132..>17579)
                     /locus_tag="PLT31_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93881.1"
                     /translation="YESVSAEYIWHPSDRFSTSLYGLMDTYKNAPYGFQLTLGGVEGL
                     YGLPSQIYAGQARFYAHLEQRYFPQFEIGTIMPVFTGFISAGQTVLEIKDFEPKEMTL
                     MAGFGIRLALTKSVAGLINHFNFSWPIKGRERSKYPTVTLTSSYSL"
BASE COUNT         6012 a         3292 c         3199 g         4966 t
ORIGIN      
        1 catcacatag gccgtttgtg cttttatttt ttcttgcagc cgagtccaat agccctcaat
       61 ttcatcgctt tgagcaacta tcggagtcgg atctgtaatc ttccatgaag aatcaaaata
      121 ctctacccaa gcatgagaat gtcgccttct aaaaagagaa tactttccat tttcagcaaa
      181 ttcaggaaaa gcaaatccta cgacatagcg tgcaggaatt ttttgatccc gtagaagtaa
      241 tgttgctaaa gtcgcgtagt actcacaata gcccgatttt gtatcccaaa aaacttgtaa
      301 ggcattattt tttttcttta tcggtaaata aagggaatat ctaaagtgag ttgcaaagta
      361 cgatcgtatg cgttttactt tttcgctgac agataaaagt gtatcaagcc ccatttctaa
      421 agatgctttt ctcactaagg caagatctct tttttcaata cttaaaaagg agcttgtaaa
      481 agcggtatct aaagaaactg agttcttaaa ataataccaa tcagatcgtt ctattcctgg
      541 agaatgccaa acccctccgc gataataata caccgaatca gccgccttta cagagactcc
      601 cgtcgcattt gaaggagcaa ataaataatg aaaagtattc aaagcggact gaacccaaac
      661 tttttctact ggattttcag tcttagattc taattcaaat aaagcgtaat cgacttcata
      721 acgttctgga taaaagacgt cataatgatt cgaaaccttc cataatcctt gaaagaatcg
      781 ttcataagct accgctttga aatagatggg aggagtagaa gtccataagc gaagaacgat
      841 ttgatcatca tatttagagt catagttttt ttcgaaagag cctaaagaag ccacaggatc
      901 aaagcccata atatgacggc tcaaataata atcttctccc cagtttccag atcttttttg
      961 agtttgattt gccttaactg tcgtagaaat aaagagagtt aaaaatgaaa taagaataac
     1021 agaagttaaa gaaataagcc atttagcaag tgataaacga ggacgaatat gccataaaaa
     1081 cactgctata aataacaccg caatgactac agaatatcgg tctggatttc gaatagaaaa
     1141 taaaaaagcc gctaatgcat taaagcgaac aaagacgagc tcgccgccgc gacctaaact
     1201 acgccattgc attagagcta aaaagagaca aaaccaagcg ggaataaaga aaagccatgg
     1261 tgagatttgc ccttcgatac ttggagataa aaaccaccaa aaagaaaaag ggataatagc
     1321 aagataaaga attcctttat gcaattgagc gacacgacca cttctttgtc gagaaaaaat
     1381 ccacgtggca aataaaaaag cagctatagc taaaaaaaca actcccgact ggtagcctaa
     1441 aaataaaata cagatactga ttaatgcaaa aatgctccat ggattcataa aaacacctct
     1501 tcttcattag aggggtcgaa atacataaga gaatcatcgc caccagtttc atctgatacg
     1561 acaatttgtt tttgaattaa gttcgaggca ttcggagaaa gagttatcgc aaaaacaggc
     1621 cccatcggaa gagcttctgg agaccatatt ccaggaggct cagatgattt aaatcgagga
     1681 gtttcgatgc gcgagagggc tgccatcact aaatccaatt tttctccgcg agtgaggtca
     1741 atttcttggt tattaataaa aaaacgccct aatatttgtc gttcttcaaa ccaggtcgcc
     1801 attgcagcag caagagcaat agctttttcc ataaaaattt tcttcgaaaa agaactaaaa
     1861 cgaatatcta aaacaaaaac gactgatgac ccttcttcta cactaaattc ttttgtaaat
     1921 ggctttagat aacgagcata agcgcgatga tcgatatcac gaacagaatc cccttctcga
     1981 taaggacgaa cgcccgagaa gtctacccct cgctgaagag atggcatcaa taataaagca
     2041 aattcacgcc caggatgccc ttttgtcaaa aaagaaaatt gactgatttt aagacgcttt
     2101 ggataaacaa gaaattcaag agagccatta taaaatacgg gcgtttgaat taagcctaaa
     2161 ggatcaggca caagagccgc aatattttta aaaatataag acccgcgatg agccgtttta
     2221 atttttaatt cgactgattc tacttcgttt gtcttaaaaa aacgagagac acaagtcatt
     2281 ttttttaagg aaggtgatgc tcgaaaagca tctatagaaa cggaaggata atcgtccaaa
     2341 gcttgaatag aagcgacaag aacgcctgtt tctttttctc gaacgggtaa aatagaaaca
     2401 gaaatgattt ttacttttgg ctttctctct cgaataataa aagaggttaa taaaagggca
     2461 aaaagaagaa ctaaaaaaaa gcgagctaaa tatgcccctg gaaccataaa taaaagtaaa
     2521 gcgatccaag cagcagaaat gagagctcgc cctgcagagg taaaacgctc aaaccaaaaa
     2581 taatagagac gaatcaaaag accttcacga taaggcgttt caggaattgc tttttgatag
     2641 gctcttaatt tttcaagcaa acgcatattt accgaggaat agggatattc tttactattt
     2701 cttggagaat cccttctgtc actccaaaac ccgattctat agcgaaaata cgatgcgcca
     2761 tcacagattt taagacaaat tgaatatctt ctggagtcac ataatctctt tcatctaaac
     2821 aagcattcgc ctgagccatt cgaagtacag caagaccggc tcttggactt gctgctagac
     2881 gaagccccgc atgagaacga gtcgcaagaa caatagagat aatatatcgt tctaaagctt
     2941 cttccacatg agtctttttt actttttctc gtaggattaa aatctgatct ggggtaatta
     3001 ctggttgtaa agagtcaaga ggccttgtaa aacgttgtga ccgtaaaata ttcaattccg
     3061 tttcttcatc aggataccca atcgtaagac gaataataaa acgatccatt tgggcttctg
     3121 gtaaagggaa aacaccatga aactccacag gattttgagt ggctaaaacc ataaaaagag
     3181 ctggcaattc gtgacgagat ccttctagtg aaacttgacg ttcttccatg gcttcaagca
     3241 aagaactttg cgttctagga gaagctctat tgatttcatc ggcaagcaaa atctgcgtaa
     3301 aaatagggcc acgatgcact tcaaatgttc cagtattttg acgataaatc gctcctccag
     3361 tcacgtctcc tgggagtaaa tctggagtaa attgaatacg cgagaaatcg gcacccacta
     3421 aaacagcaag agcttttgcg agagttgttt taccagtccc aggaacatct tctattaaaa
     3481 cgtgaccatc cgctaaaaga gcatttaata atagacggat ttctttttct ttacctaata
     3541 agacatgatt cagtgaatta attaattgtt ttagcataaa attaaattag cttttattcg
     3601 ctttcattca cgattaaaaa tcaaaagaaa aaaaacaatg tgatatgact ccagactatt
     3661 ttttcttgac aaatatgctt gaaaagcaaa tagacttttt tcaagaatta aaacgatact
     3721 tactattttt caaaacattc tattttctca aattcctttt tcattaaatt cacaatcgtt
     3781 tcattatgaa acatttcatt tttttcttat cacaaaacct ttttttttga aataaaacgg
     3841 cattttactt tttaggcacg ctttttgctt atcnnnnnnn nnnggctgac caagaaacca
     3901 gtaaaaacat aaatccagaa gaagatgatc ttattcgtga agcagaagct tgtgcttgtg
     3961 caaacgaaga ggcttgtaca gataagactt cagctgatcc tgatgtaaaa gaagaagaaa
     4021 acaaggaaaa agaatcttct gaaaacccag cttctgaaat tgatgtttta aaagcagctc
     4081 tatctgcatc taacgataga tacttacgtt taatggctga attcgacaac tatcgtcgcc
     4141 gtacagctaa agaacagctt gaaattattg aaaccgcgaa cgctaaacta ttagaacaac
     4201 tctctgaagt tttagataac tttgaacgag cttcccttcc agaaaatcgt ggatgcgata
     4261 tcgaagcctt tgcaaaaggg atgagcatga ttcacgatca atttgaaaaa actcttcgtg
     4321 atgcaggcct tgaacaaatc aatcctgttg gagaattatt tgatccttct tgccaagaag
     4381 ctcttataaa gcaaccttcc gaagatattg aagaaaacca tgtgattcag gtattccaaa
     4441 aagggtaccg cttaaaacat aaaatcatta aaacggcaaa agtcatcgtc tctgccggga
     4501 aataacattt ttaaccttat ggactatttc aaaatgaaac agccttttca aaaaacctcg
     4561 ttttttgctc aaaaagctaa aaatcagatt ggcacgcatt ttgccttaga tccaatgaaa
     4621 gaaattaatt aggagatact cattatgagc aaaattatag gtatagatct tggtacaaca
     4681 aacagctgcg tctctgttat ggaaggcggt aagccagttg tgattgctaa tgctgaaggt
     4741 tttcgcacga ccccatctat tgttgctttt gcaaaaaaca acgaaacgct agttggacat
     4801 gttgcaaaac gtcaagccat tactaaccct gaaaagacaa tctattcaat caaacgtttt
     4861 atgggccgca ctgcagggga atgctctgat tcagaaaaga acatgcctta ccatcttgta
     4921 ggcactggtt ctagccctgt tcgcgttaaa attgatgata aagaatatgc accaccagaa
     4981 atttctgcaa tgattcttca agccatgaag aaaatcgctg aagattatct tggacaaagc
     5041 ataactcaag cagtgattac tgttcctgct tattttaatg acgctcaacg tcaagctaca
     5101 aaagacgctg gtaaaatcgc tggattagaa gtgcttcgta ttgtgaacga acctactgct
     5161 gctgctttag cttatggttt agattctaag aaaagtgaaa aggtcgctgt ctatgactta
     5221 ggtggtggta cttttgatat ttctattttg gaaattgatg aaggtatgtt tacagttaaa
     5281 gcaaccaatg gcgataccat gcttggtggc gataactttg atgaaaccat catcaactgg
     5341 attaacgatg agttcaagaa gagtaatcca ggaatcgatc ttaaatcaga taaaatggct
     5401 ctccaacgtt taaaagatgc tgctgaaaaa gcaaaaattg acctttctgc gaccacaagc
     5461 acatcgatta atttgccatt tattactgca gatgccactg gtcctaaaca tcttgatctt
     5521 actctttcaa gagcaaaatt tgatcaattg accgcttctc tcgtagaacg ttctattgaa
     5581 ccatgccgta agtgcttagc tgattctggc ctaactcttg gtcaaattga tgaagtgatt
     5641 ctcgtcggtg gttctactcg tattcctgcg gttcaagaag ctgttaagaa gttctttaat
     5701 aaagaacctc ataaaggtgt aaacccagat gaagttgtgg ctgtaggtgc tgcagtgcaa
     5761 ggtgctgttc tttctggtga ttccgctgtg aaagacgtgt tacttttaga tgtaacccct
     5821 ctatctcttg gtatcgaaac ccttggtggc gtgatgacta agttgatcga acgcaatacc
     5881 actgttccta caaagaagag ccaggtgttc tctactgctg aagacaacca gccagcggta
     5941 acaatccatg ttcttcaagg agaacgtgaa tttgcacgcg acaacagaac attaggccgt
     6001 tttgatttgc aaggaattcc tgctaaacca cgtggtgttc ctcaaatcga agtgactttt
     6061 gacattgacg caaacggcat tgtgcatgta agtgccaaag acaaagaaac tggtaaagaa
     6121 caatccatta agattacttc ttcaagtggc ctttctgaag atgaaattaa ccgcatggtg
     6181 aaagaagcgg agatgaattc tcaaaaagat aaagaagcaa gagaacttgt cgacatcaaa
     6241 aaccaagcgg aacaaatggc ttatcaagca gaaagccaga ttaaggaagc tggagataaa
     6301 atccctgcgg acaccaaatc tcaattagaa gccgcgattc aagaaatcaa agacaagaaa
     6361 gaaaacggta ctaaagaaga gatcaaggcc gcgatggata agttgcagaa catgctcggt
     6421 tctatggctc aagctgctgg agctggcgct caaccaaatc caggtgctgg tgattgtggc
     6481 ggagctggat gcaatgcaga accagaatcc gataagaaaa aagatggtcc aattgatgca
     6541 gaatacgaag tcgttgatga cgataagaaa taatcaaagt gacttaactt aaagccccta
     6601 acacaaaaac gggattctct ttattaaaaa aaagaggaat ccctttttgc gtataaaaca
     6661 caagtttaga ttttatcgat agagatattt attatggcta caaaacgcga ttattatgaa
     6721 gtccttggag tgtctaaaga tgcaagtgct gaagagctca agcgcgctta taagaaacta
     6781 gcaatcaaat accacccaga taaaaaccct ggcgacaagg aagcagaaga aaaattcaaa
     6841 gaagctgctg aagcttatga cgttctttcc gatgcaaaca aaagaagtca atatgatcgt
     6901 tttggtcatg acatgcctaa cgctggtggc tttggtggtg gtcaaggatt ctcaagcttt
     6961 gaagatattt tcagtcaatt tggagatatt tttggaggtt ttggctttgg ttcttctgga
     7021 agaggaggac gttcagcaaa aaaagctggt ccaccacgag gacaagattt acagattaaa
     7081 gtggcacttt cttataaaga aattttagaa ggcgttacta aaaaagtacg tatcaaacgc
     7141 tatgcccctt gtacttattg taatggtaaa ggcgggtctg atgtgaccac ttgtagcact
     7201 tgtaatggaa caggacgagt tcgtcgtgta actcaatcgt tcttccagat ggtgagcgag
     7261 tccgcttgcc ctacttgtaa tggcatgggt gaagtgataa aaaatccttg ttctcattgc
     7321 cgtggcgaag gtcgtgtttt agaagaagag atgatctcta taaagattcc tgtgggtgtt
     7381 gccgaaggcc agtacctcaa tatgagaggc gaaggaaact gtgggcctag aggtggcgct
     7441 agtggagatc ttttagttgt tattactgaa aagaaagatg acttctatac tcgtgaagga
     7501 gatgatttac actgcgatat ccaagtccca attcacagac tcgttcttgg tggttctcaa
     7561 agaattccta ctctagaagg tggcgaactc agtattaaaa tcagttctgg cacacaagtc
     7621 ggaagtattt taaggctaag agaacaaggg ctttatcctt tgaatggtcg aggaggtcgc
     7681 gggaatctat ttgtaacgat tcaagccgat atccctaaag acttatcttc taaagaaaag
     7741 caactctatc aagaactagc tgaaattcga aagaaaaaag aagacgaaca agaagaaagc
     7801 tttattcaaa aaatgaagaa aatgttttct taaaataaaa gttacttctt agttttttcc
     7861 aaaaaattag gtgtaatcat ttgtcattgg ctgcaaacca ttacgcctgt tagctcttca
     7921 aagagacaaa taccaaaagt atttgtcttt ttttttgatt aaaaaacttt gccaatactt
     7981 gtataaaaaa gatttcgtca tttctaccaa aacgaattca tgtgacgttg aagtttatta
     8041 agtactctat aacaaatgaa acaatgagtt aaagcgacgg gtaaaaaaca attgtaactc
     8101 attatgcaat cattttaaaa cgcactttta ttacaaggga atatgattcg aaataacttt
     8161 tagacgtttc taataaaaca aattcatgtg acgttgatgt ttttataaag tactctataa
     8221 caaacgaaac aatgagttaa agcgacgggt aaatatgact ttttgggggt gtaagaaaat
     8281 tccacgagat tctccccgtt ttgaggaaga acttaagaat atcttatgtc tgtaatttgg
     8341 caaaatgtac attcgttccc tttattagga gaatgttaca ttttaacgcc tttttttgat
     8401 taaaactgga aataaaggaa tggattgtat gactgcgtaa atagaacgag acaagcaaaa
     8461 acaaagagaa agagcgccac ggaaattcgg cgaacattga gtttttggca ccattcaaag
     8521 gcacgccaag gagaaatggc gtagaataag gcgataatca taaagaagca gttcaacgga
     8581 gtaaaaatct gtgccgttaa caaaggatct gctgcggaaa caggcattaa accgagcaaa
     8641 gacttccaca tggaaatggc ctctccaatc gtattcgctc taaaaagaac ccatccaaat
     8701 aagacaatca cctgagtaat taaaacctga aaaacaaaag gaactttttt ataaatagct
     8761 tgtttaccgt tgtatctttc tacgatcata aagaaggcgt gatacaaacc ccaagcaata
     8821 aaagtccaat tggctccatg ccaaaagcca cttaagaaca tcacaacgaa taaattaaaa
     8881 taaagcctac gtggactcac tcggtttcca ccaagagcta tataaagata atctcttagc
     8941 caagtagtta aagaaatatg ccaacgagtc caaaattcgg taatgctcac tgatcgatag
     9001 gggccattaa agttacgagg aaaatgaaag cctatcatta aacctaaacc cacagccata
     9061 tcagaatagg ctgaaaagtc aaagtagatt tggaacatgt aggcaatact accccaccaa
     9121 gaatttaaga tgccaggagc atcacttgca aannnnnnnn nnnnnnnnnn nnnnnnnnnn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnnnnnnnnn nntgtaggca atactacccc accaagaatt taagatgcca ggagcatcac
     9301 ttgcaaaaac tcgatcggca ataatcccca cctgatttgc aatcaaaatt ttcttagcaa
     9361 aaccaataca gaagaaagta atccctttta gaaacaaatc catggaatgt tttctagaag
     9421 ctaattcttc tgcaacggtg ttataacgaa cgatagggcc cgccaccaat tgagggaata
     9481 gagctacata acatgaaaat gaggccaagt tctttgcagg aggagccacg cctctccata
     9541 catcaatagt ataactcata gattggaatg tatagaatga aattccaacg ggtaaaataa
     9601 cgttccatac agaaaaagtg tctataccga atttggataa aatcgcattt agcccaccca
     9661 taaagaacat gtagtatttg aaaaagccaa gggttgataa attggcgaca atggaagccg
     9721 ttaaagcaat attacgttgt ctttgacttg caccctctgc cgaaataaaa cggccagcta
     9781 tataatcaat cgttgtggaa ctaaacatta aaacgataag ccaaggttcc atccaaccat
     9841 aaaagatata actgaatatg gtaataaata aattgaggta agaatgccta acgccccgcc
     9901 aaacaagaac aaaataccca atcaaaaaaa cgggaaggaa atagaataaa aaaacttgag
     9961 aagaaaaaac cataagttat tcagtcactt ctattgcaaa ataacgaatg gattcatcat
    10021 caaaatactc gtcttcaaga gcttcttgcc aaactgattc tagcggtact agtacaagac
    10081 gatgtttcgc accagcctta aactcacgta cattgccttt agaattagca gccattttgt
    10141 ctttgacttg ccctcttgga atttttgggt tataaacgcc aacgagaact tctttttcct
    10201 ttaaggagcc tttaactaca tttaaaactt tatacttaaa aacataaaca taattataga
    10261 gatcattacc aggaatttta ccaggaattt cagttaatct ggcatcgacc acaatagggt
    10321 ccgcagaata agcaagaaca aaactcatca ataaaagaaa caatattttt ttcatatgaa
    10381 ccttttgaat gagcattaaa atagaattat tctcataaaa aattgaggca ctcaattatt
    10441 ttttattata aaaaagcctt ttcttctaat ggcttagaat aagctcgacc agatgaaaga
    10501 aaaaaaggca ggtaacggct gcttttctaa aatgctaata aaagaatatt aaaaacttaa
    10561 atatacagtc tagaattgct aaattgtcca tggagtaaaa ttcatggaaa agctttggaa
    10621 taaaccattt atccttatat gcatctcaaa ttttttgatg ttcttttcct tttattcttt
    10681 actcccaatt ctaccccttt acatctttga aaaattaaac gctggtagta cggtctctgg
    10741 tattatttta gcgtcttaca ccattggtgc tctattatgt aggccttttg ctgggtatct
    10801 cgtcgatagc ttttccagaa agcaccttta cttatttacc tatatccttt tttcattttt
    10861 atttcttggt tatttagtca cctttactct tgtcttaatc actgccacgc gtttaattca
    10921 tgggctttcc tttggtattg ttaccacagc aagtaataca attgcagttg acgtattacc
    10981 ctcttctaaa agaggtgctg gtattggtta ctttggagtc accaccaatg tcgcttttgc
    11041 cctcggccct atgacaggga tgctgctata tgaatcgtta ggttttaatg cggttttcat
    11101 tttttctttt cttacttgtt tgattggaat tgttttgatt tatttcttaa aagtaccaat
    11161 gaagcctata aagaaggacg cgagccctat ttcactagat cgatttttcc tcgtcaaagc
    11221 cacgcctcaa tttatcaatc tcattttaat aggttatgcc tatggaccta ttacgaatta
    11281 cctggctctt tatgccaaag aaatcggaat agaaaaaggg attggttatt tttatgcttg
    11341 gcttgctatt ggtcttgtta gtagtcgtct tatcagtagt cgctggattg atagaggcta
    11401 tcttaccaca ttaataaaaa tagggttagt gattttaact tttacctatg gatcattttt
    11461 ctggatccac tcgccatttt tatttttcct tctcgccttt attctcggga taggccacgg
    11521 tttagttggc ccagggtacc aaacgatggt agtgaatatg gctccgcatt gtaaaagagg
    11581 aacagcctct agcacttatc tttcggcttg ggatttaggg attggaattg gcattttact
    11641 tggtgctcca atcgctacgc atatccaata tagcggtgtt ttcttattag gagccttgag
    11701 tgtttttatc gctctaattt tatttgttac tatttctact cctcattacc aaaaaaatcg
    11761 attggaataa aaaaatggat ttaacacaaa ttccaggcct tggtccaaag agaattcaaa
    11821 aactgaatca agctggtttt tatagtattc aagatttatt gtatcatatt ccaaggactt
    11881 ggctagatca aactaaaatt cattctataa aagagcttaa agcgcaagat aaagccattg
    11941 tcattgggca tattgttcga gcaggaatta ttcgccgtcg ctcctccttc tttcaagctg
    12001 ttttagccga tgattctggc caaatcactt tgatgttttt taagaatccc tcttactggt
    12061 ccaaaaaaat tatgcctggg atgcgctttg tggctattgg aacagtcagc gagtatcgag
    12121 ggcttcaact ggttcaccct gaattgcagc cgatggaaga tgatgccgat tttaagggag
    12181 gcattgttcc tgtgtattcc attccagaag tgtgccgtga agcacgtatg gaacaacgtt
    12241 ttttcataaa gctctataaa tatcttttta ctcttcccca cttaagtgtt tccaaagctt
    12301 gtccagatga actttgcact tttttaggat tagttcccac aatcgaaaac cttaaacgcc
    12361 ttcatcaacc aaaatctttt ggtgaagcca tgcaagggcg acgccagctc aaaattctag
    12421 agctgctccc tttctgttta cgaatggcct caagacgtag gcatttgtca cttagagggt
    12481 ctgagcgtgc aattgatcaa gggcttgtca tggccgccga agcaagttta ccatacgaac
    12541 ttactaatga tcaaaaaaaa gctctcgctc aaattttaga agggttacac ggcaaaaaac
    12601 aatttcatgc tcttttacaa ggagatgtcg gtagcggaaa aacggtcgtc gccttactcg
    12661 ccatgctttc agtttgtggt gctcaagaac aatgtgcttt aatggttcca acagatatct
    12721 tagcgaggca acattacgct tttctaaaac cttattttga aaaagcggag cttagcattt
    12781 ctcttttagt cggagcgaca agtgctgccg aaagaaaaac gattttagga gagcttcaaa
    12841 tggggctttc tcacgccgtt gttggaactc acgcattatt ttctaaagat gtctttttta
    12901 ataaactcgg gtttgtcatt atagacgaac agcatcgttt tggcgtaaat caacgagaag
    12961 ctcttttagc aaaagggaat taccccgatt tgttagtcat gagcgcaacg ccaattccaa
    13021 gaagcttagc catgacattt tatggcgact tacagacgat tttaatcaaa gaaaaacctc
    13081 ctggtcgaaa gcctgtaaaa acaagattaa tcaccccaga aaaacgggat gatatgaaaa
    13141 actttatttt tcaggaaacc ttaaaaggga atcattgcta ttgggttgtg aatcgagtag
    13201 aaaacgatga agaaggtaaa tccctttccg ttcacgatat ttttgaagag ttacattctt
    13261 ggaataaaga ttggaaagta gaagtgattc acgggcaaat ggatgaagat cttcgtaacc
    13321 aaattcttaa agattttaat gcgggcctga ttcatgttct tattgccacc accgttatag
    13381 aagtgggcgt aaacgttcct tcggcaaact taatggttat agaccatcca gaacgtcttg
    13441 ggctttctca gcttcatcaa ttacgaggcc gcgttggccg tggcggagat caagcgtggt
    13501 gctttttaat gtgtgactct aataaccctg cgatagagcg actcaatgga tttgcaagta
    13561 ccgaagatgg ctttgaaatt gctgagatgg acttaagaga ccgtggggct ggaaatcttg
    13621 aaggaaacga gcaaagtggt tcttggttat tccgcttttt tgactggatt gaagaccaga
    13681 atttaataca aaaaatacta gaatgggccg aacaaatttt agacaatcaa aatcaattcg
    13741 acaaagtagt tatagaaaag attcagcagt ggtatagcga actacctcaa ggaaatgaag
    13801 atggcattca ttaacttgaa gtgcttatta ttctaatcct aacacagtga ttttttcaaa
    13861 agtacggccc tttttaacat aataaatccc agaatgcact ggttttacat gccctagtct
    13921 atgccctttt acgctaaaat ataaaggcaa atcataactt gtattttgta attctaattt
    13981 tgaattgaat ggtacaaaag tcataatatc tttagaaata tcaatattca tagcgtcaat
    14041 ttttgtatta aaaaaggttt gcctttctgt taaccaagtt ttcaatttat tcacatgatc
    14101 tgttttataa ctatcataag aagatggata agtagcacca aaaccaccaa agccgccaaa
    14161 gccgccaaaa ccaccgccgc cactgctagc accagaagac aatacattaa agttattcat
    14221 tatagaaggc tctaaatacg ttgcaataga atctataaaa acacctaaat tattaatatc
    14281 tgtttgatgt gcatcccata attttttcca tttggcaagg aatacaggat catcaaaaaa
    14341 acgtttataa taactatgcg tagggatcat attatgatga ttatcatcgc ctgtagtggt
    14401 aaaatgctct ggagaaaatg aaaaggtaat acctgcggaa aggtcgaaat cccaaagagg
    14461 cccagccgta attttacccc caacatcttt gtaatgaaag ttagaacctg gtaaaaagcc
    14521 aggttgagct tggctattaa aatcaaaatt atctaaaaat ttttgaatca tgatatactt
    14581 tgccatggcg tctaaatcca ctaaatcacg atacccattt tcaggaaaat cagatgcaaa
    14641 catctttgta gataattcat ctaaatctct tttaatccaa tcgatattcg ctgattttgg
    14701 atcgtcataa tctggacttt taacaaaagt tccaagagag tcttcaccat ttaatccatt
    14761 ccatttccat tgaaacctta tttcatctgc ttctggagcg tgataatcaa attcaactag
    14821 ccagccatct tttttactta tattaattct attttcattc acctgaattt gatcacagag
    14881 caaataaagc cctttatacg aattattgat atagacatct acaaaacgat aatcgggagt
    14941 aaagtctaat ccaaaacgtt tgcctaattc aaaggcaagt gcattaagaa tctgtgtatt
    15001 atcgtagtaa ttcgcaagta atacccaact ttttgcagca ggtagttcaa atagcgatgt
    15061 tttcttgtca aactttattc gatatggttt tttggaagca tctgaagtag aattaccgcg
    15121 caccttaatc gaatctacgt ttcctgtttt tgaaagatta tggacaggat tatcagaatc
    15181 acttaactcc atgttcatga catagtaatt tttttcagta acatcacttc cgttttgtgt
    15241 tgtgatttta atagtaggaa tagctgcaaa agcaaccatt gaaaaaatga ataaaataaa
    15301 acagatattc gaaattttta aatattgcat ttaaacaata taaatctatt tcgatgactc
    15361 tcttccctct taagggagag agaaaagaat aacgttgtaa aaaattgtag aaaaaagaat
    15421 ggatgtaaaa ttatttcaac aacaatgcat ttatttaaga aaatcaataa taaataatta
    15481 aatacaaatt caaaaaatta atttatcaat agtattatac ccatgcctgc aatatagcca
    15541 aaaacagcaa atggcgtaat ccaacgtaaa taccatccaa aagaaatttt ttcaataccc
    15601 atagtagcaa caccagccgc agagcctata attaataagc taccacctgt acctgcacaa
    15661 taagcaatta aagaccaagg agtggcatcc attggaaaat cgtacatacc catgcaagca
    15721 gcaactaaag gaacgttatc cactatagca cttaaaagac ctaaagagaa agtgactaaa
    15781 tgaatattgc ctaacgtatc ttgtaaaaag tcagcaaaca tttttaattg ccctgtacat
    15841 tctaaagtcg ataccgcaaa caaaatacca gcaaagaaaa gaatactaga catatccact
    15901 ttttttaata tggaggaaat ggaatatttt gttttaaaat cattatcttt tttgcgtaat
    15961 aaaagatccg tcactagcca taacaaacct aatgagaaaa gcatgcccat ataaggagga
    16021 atatctaata gagcttttaa aacgggaact aaaattaaac caaacatacc gaaaaagaac
    16081 actacatatt gatccatacg ttccgtggaa atagtttctg aatcagaatt tttttctctt
    16141 actggaattg tccctttaag caaaaaggtc atcatcaata aaggaattaa aacagcaaat
    16201 acacttggta agaacgtgct tttcataatt ccagcaccag aaataaggcc tttaatccaa
    16261 agcatggtgg tggtaacatc accaatagga gaaaaaacac cacccgcatt tgcagcaatg
    16321 ataatcattc cagcaaagaa tttacgatct tttgctttta ataaaattct acgcacaaga
    16381 gtcaccataa taattgatga cgtcatatta tctaatacgg cagacataaa aaatgtaatg
    16441 aacgtaattt gccaaagtaa gacacgaaca ttatttgttt ttattcgatt ggtaattaaa
    16501 gaaaatccat cgtaagaatc aaccacaaca acgatggaca tggctcccat caaaaaaaat
    16561 aaaacagagg cgacagagcc cagagattct gttaattgag aaccagcttc tactggatta
    16621 tttgaaaaga gaaaaaatag aagccagaga atgccaccta aaaacatggc tgttgcagat
    16681 ttatttaagc gaataggatg ctctaaagca atgagaacgt agccgacaac gaaaagaatt
    16741 ggaaatagga acatacagaa aatcctttaa aaaaggccta ttgtctcgca attctagtaa
    16801 aaagtaatcc ccaaggctag gctaaataat taaaatgaaa ttaagcgggt taacattcca
    16861 atcactaaac tggaaagcat taaatttaag aataaaaaag tacgccgtaa aatggtaaaa
    16921 aaaacattca aagtgtgtat tttattccca ggattcttta tttcttttat agcaagtaat
    16981 acacttaaaa agcccgtgac cacaagcatt aatgcaccta caaaatagcc ttcatcccag
    17041 acaagaagag tcagaacaag gcctgtgatc atcgcaatgg ctccaataat gatctcaaaa
    17101 atggtaaagc gaattttcat aaagcctctt tttataaaga ataactgctt gtcaatgtaa
    17161 ctgtagggta tttactacgt tcacggcctt taatgggcca actaaaatta aaatgattga
    17221 ttaaaccagc gacggattta gttaaagcta agcgaattcc aaaaccagcc attaaagtca
    17281 tttctttggg ttcaaaatct ttaatctcta aaacagtttg accagcacta ataaagcctg
    17341 taaaaacagg cataatcgtt cctatctcaa attgcggaaa ataacgttgt tcaagatgag
    17401 cataaaaacg ggcttgacca gcataaattt gagaaggcaa accatataaa ccttctacac
    17461 cccccaaagt caattgaaaa ccataaggtg cgtttttata ggtatccatc aacccataca
    17521 aggaggtcga aaaccgatca cttggatgcc aaatatattc agcagaaact gattcataa
//
LOCUS       DAOOBB010000018        17290 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_4290_length_17290_cov_4.636321, whole genome shotgun sequence.
ACCESSION   DAOOBB010000018 DAOOBB010000000
VERSION     DAOOBB010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 17290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17290
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4290_length_17290_cov_4.636321"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="PLT31_01720"
     CDS             complement(<1..899)
                     /locus_tag="PLT31_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPW93882.1"
                     /translation="MSQYKKILDPKEVVATLPDGRTIRLETGRLAKQAAGSAVVQMGD
                     ALLLATVCFGPDKEADFFPLTVEYREKAYAAGRLPGGYSKREGGRPSDEETLSARIID
                     RPIRPMFPENFSREVQVIVQALSADKKFAPDVLGVSAASLAIGLSELPFEEQVAAVRV
                     AVVDGNPIVCPSYEEVAISDLDLVVAGTANSVCMVEGGAYEVSEDTMINAILAGHDAI
                     KILCKAQQELVDRCAKEKMVLPPKDVSEAHEKLAATIKEVIYTELYNTLHTPMVKTDH
                     YPAMAALEEKALADERVTTLIGE"
     gene            complement(918..1190)
                     /gene="rpsO"
                     /locus_tag="PLT31_01725"
     CDS             complement(918..1190)
                     /gene="rpsO"
                     /locus_tag="PLT31_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPW93883.1"
                     /translation="MATITTEKSQEITAAFGANAKDTGSARVQIAILTEKIKNLTEHA
                     KMHRKDFHSLRGLTAMVAKRKNLLKFYSKQDLEGYRALVKELGLRG"
     gene            1394..2158
                     /locus_tag="PLT31_01730"
     CDS             1394..2158
                     /locus_tag="PLT31_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93884.1"
                     /translation="MIDKLNLSFGFYDYLLIFAVAALGTFSAYLKDPQLKAVCATIPI
                     PFGFAYLALEMPIGAANAGSALLCLLFVHIVRYLTYKKNFHVILSIILGICVFIFIGA
                     MLLPYLPTQEWFFLLICALDFGLGLLIFQKQSYRHGVRHKTPLPVYIKAPAVAAVVAM
                     LMVIKKILGGFATSFPMMNSIVSYESRHSLGDQCRQLPLFLIAGPFMFIVMRYTELLL
                     HFNRWIVLAIGVTLYMALFYPLNKELKRRNERSSRA"
     gene            complement(2439..3767)
                     /locus_tag="PLT31_01735"
     CDS             complement(2439..3767)
                     /locus_tag="PLT31_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HPW93885.1"
                     /translation="MDIQEAVLHLKEIASKKVEQFDIIAGRSLSEGLSLFQSKVQNTE
                     ISESVGLGIRVFKNGSPGYAHTERLTPAAISQTLEDALAHTAFTEALPIDLPSPETLT
                     DIDGLYNPELGKISLSEMTSFCLDVEASVFKASSEIENVPYLGADRSESAIWIANHKG
                     VFYERHRNSISVGVGSVASRDGIKKVGIYSKSGMDWSQFDPSFIASQSVERALELLSP
                     SPIQKGNHPVVFSNRVSGSIISMFSSSFYADTIQKGQSRLQDLYHEKIAPDFFNLSSD
                     PWRTDLPGSCSFDGEGVPTQKIEVVQSGVFSNKLYNLESASRDRVLSSGNASRSYNGK
                     VETSFKNIVVAPGTYSHEALLNLFPKCLYIVKLEGNSGCSAVSGEMSIGVQGIWVENG
                     KPVHAVEGLTLSINFFDLLKRLVALGNTYNDSFSSVKVPALAVSEVAISN"
     gene            complement(3772..5052)
                     /gene="hemL"
                     /locus_tag="PLT31_01740"
     CDS             complement(3772..5052)
                     /gene="hemL"
                     /locus_tag="PLT31_01740"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HPW93886.1"
                     /translation="MSQTDLWLKACEKIPGGVNSPVRAFGGVGGTPIFISRAQGPFIY
                     DIEDREYIDLVLSFGPMILGHNPSVVTEALEKQLKKGLSFGACTGAEVLLADRILSMV
                     QGMDLVRLVNSGTEATMSAVRVARGFTGRDKIVKFKGCYHGHGDSFLIQAGSGALTHG
                     VPSSLGVTQGVAKDTLVASYNHLEEVEVLFEKHSHEIAAVIVEPVAGNMGVVTPAKGF
                     LEGLRRLCDASGAVLIFDEVMTGFRLARGGASELFAVKPDMMTLGKIVGGGLPLAAYA
                     GRREIMEMVSPLGGVYQAGTLSGNPMATAAGLAQLDLLEQEKPWAELALQGSRLAQGL
                     RDAAATARVPVQVNQIGSMITVFFSDKPVIDTETAMAANASTFARFFHGMLERGIYLP
                     PSQYESAFISTMHNDIILNQIIEKASEVFSIIGK"
     gene            complement(5049..5288)
                     /locus_tag="PLT31_01745"
     CDS             complement(5049..5288)
                     /locus_tag="PLT31_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HPW93887.1"
                     /translation="MTNRRKIQPPGVTIEESIHFLKMSRSDFARRLEVDESFLNRLIA
                     GEEQITPSLSERLEALTGSPSNFWKMLESKYRRSL"
     gene            complement(5296..6762)
                     /locus_tag="PLT31_01750"
     CDS             complement(5296..6762)
                     /locus_tag="PLT31_01750"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93888.1"
                     /translation="MNRIAIFFIILFVGILCVPASTQLFYSLNKDVAFQPLDMITDIF
                     KTPFNRESDLRQASDSLNKTWQSVLNKKEFDALSLEPVLDAFRFLKTSYTNTNSYTTM
                     DSSSIKDQDLLKIESLLSELEDNPSVSDSLTPLIKQASLSYGSFSIKRLAEYYWHYGF
                     LTSRYWRPYEKRLEDENKVTQKSRVLIQRSFWTLFNEPGEKAFLGEDDWLFYRDDVAY
                     LTMPSPLDKNRSKHLDNPVKEIVAFQKDLQKKGIELLVVIIPGKPSIYPELLTSSWDS
                     SSGVPFTHSQDVLNALNEKGIKTVDLYKPFLEAKKEDQDSNYLYLYTDTHWTPRGAKL
                     AASVVAEKVRSMEMDLPVNYYKEDTTLVERVGDVAVMTSLPVFPSQWVKAYPVKDSSG
                     VLYKDNYRQSSILILGDSFSRIYQTDAPRSAGFISHLAKELNTPLSSIVSDGGASTLV
                     RETLARRSGVLKGKKLVIWEFVERDLRFGAEGWKRISL"
     gene            6963..9026
                     /locus_tag="PLT31_01755"
     CDS             6963..9026
                     /locus_tag="PLT31_01755"
                     /inference="COORDINATES: protein motif:HMM:NF023853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HPW93889.1"
                     /translation="MSIQDDFGSLVFNAKTMSKYLSSQTYQSLLATMHNGTDLDPSIA
                     DEVAYGMKNWALDQGATHFTHWFQPLNGTTAEKHDSFFNPDYEGGVIASFSGKELSQS
                     EPDASSFPSGGLRATFEARGYTAWDPTSPAFIRKSQGNVSVLCIPSVFCGYHGEALDK
                     KTPLLRSQAAIIKQVYRVAKLFNLPMDKRPYITLGAEQEYFLIDEAVYKMRPDLMQTG
                     RTLFGIRPARHQQMEDHYFGKINPRVLNFMADVDHELWKLGIPAKTRHNEVSPAQFEF
                     APVFEEQNLAVDHNMLTMSVLQSIAERHGFICITHEKPFAGVNGSGKHNNWSITGPDG
                     KNWLTPGKTPHENAKFLFVLVAIIKAVDTYAPLIRAAVASAGNDHRLGANEAPPAIVS
                     IFLGEQLTDIINQLECGTPKSSKCGGLLEIGVSSLPKLPKDATDRNRTSPFAFTGNKF
                     ELRAVGSDQSCASSNTVLNLSVASTLDEMITEMEKAITAGEDFNIALQNILQKEIKAH
                     KRILFDGNGYSDEWRDEAARRGLPNLKSTPDAIQAFKDPKFIALYEKYSVLSEKELNS
                     RYAIAKETYERVITMEAECALMIGKTMILPVGISYAADLANSLSSLSKVEHSALQKMV
                     EATASLVNSLIEAIEHLETSIKSGHADQMLTAMDKTRQFADNLEEIVPDNQWPLPSYG
                     DMFFI"
     gene            complement(9164..9970)
                     /locus_tag="PLT31_01760"
     CDS             complement(9164..9970)
                     /locus_tag="PLT31_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW93890.1"
                     /translation="MKLKFLLVFVLFSIIFMSGCSSVTALRTKELRVVGNVVRKDVKD
                     EMNLQVDSLKQVIDSLYKAQDLVNRRLMAELSILSKRVASESERNDSRQEEILYRLDM
                     LLGKSDKILAKKVVINKSSSEPLALDSIEQEAERLLEAESMFNTGRSDYLRGEYKLAY
                     STFKQVYELMKTGEFAEESLYWMALCLVQVDQKDKARILFSGFVEQFPNSTKICTVLF
                     KLSNMAAEENNIPSQKQYLQKLLEVKSCMESNEFLQGAAILEELLNQEKK"
     gene            complement(10004..10573)
                     /locus_tag="PLT31_01765"
     CDS             complement(10004..10573)
                     /locus_tag="PLT31_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPW93891.1"
                     /translation="MKSIYALLIAGCVVIGLTACSKKKEPVVDPVVKETPAEAVAPEQ
                     SKVNEDSLAALERERLEAERLEAARKKLQEMMDRVMSEDIYFDFDRSELTEDAKQLLT
                     QVGEILNQEPRFTIIVEGHTDARGTEDYNMTLGSARSTKVKEFLSAYGIASERMETIS
                     YGEEKPKQDGESEDAYAKNRRANFRVNVK"
     gene            complement(10570..11865)
                     /locus_tag="PLT31_01770"
     CDS             complement(10570..11865)
                     /locus_tag="PLT31_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation protein TolB"
                     /protein_id="HPW93892.1"
                     /translation="MNKSLFLALFLSMTMQVFASIDTIAVDVGISVFKTMPIGVVPFS
                     EPSGTTLDWPDEKPHEVAERDLNLSGRFDVISVPKFDLVKLSRARAKFYVTGKIEKAL
                     NNKIKIDFYLYATQSKDLVLGESYTVSLIDSRKAVHDFIDKVIYNLWGVHGIASTKLA
                     WVSKIDGVKQIMVADYDGFHRRQLTFDSTISMMPAWSPDNERIIFVSFRNNKPQLYIK
                     DVATGTQKRLFKKFEQTFSPAVNPKTGEILFSAIIDGKTDLYVGDPETQKTRRISFLK
                     SSQTSPSWSPNLSEIIFTSDRGGSPQIYVMNSDGSDMRRMTFMGRYNERASWAPTGDR
                     IVYTSMDDGKMNIYTCAIDGSDIIQLTSNSGNNERPSWSPDGMLIAFSSNRSGSNQIY
                     IMRRDGSNVTRITQGGDNTAPTWSWYPIENQTKQKEGKE"
     gene            complement(11914..12621)
                     /locus_tag="PLT31_01775"
     CDS             complement(11914..12621)
                     /locus_tag="PLT31_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPW93893.1"
                     /translation="MTDKGSLYFSSGINSSITKIVVVSLVFHLLCIAALFIVNNINFK
                     SEPEPIPVFEMVQVAAPVPRQVVHRPKPKVEPPKPKVKKELPPEPKQEEPKPEEPKPE
                     PVEEVVEETTEEVTDDFPIDDMDLPTMDAPSLHPVGSVDMDPLMQVYLERLKQIIMQN
                     FNPPNGMNIGKDAKTTVQFTVDRFGLISNITLKRSSGNKTWDHLSMRAIQIAKVPELP
                     PNYRAPALSLNFNFTPK"
     gene            complement(12611..13024)
                     /locus_tag="PLT31_01780"
     CDS             complement(12611..13024)
                     /locus_tag="PLT31_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPW93894.1"
                     /translation="MKRSRGKTLNQEMNLTNMIDVVFSILIVFMISAPLMSQGVKVDL
                     PKAEAPTIEQEKLLKVSITKDDDLYIADMKVDMKSFNGVFKSLWNGEMAVVINADETV
                     NYGLVMKVVSAVQKAGVTKLGFLTLSPKENLKNDR"
     gene            complement(13033..13722)
                     /locus_tag="PLT31_01785"
     CDS             complement(13033..13722)
                     /locus_tag="PLT31_01785"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPW93895.1"
                     /translation="MLSLPIVQMIMKADVATIVIIAILAVMSFGSWGIIIVKLFSYRK
                     NQRANASFFSRFNQVEHFIELQGLCETSGDSALKRLTSEVLIEAAKFSNFVSYDSIQH
                     RASLLEDTIQRSIEGLRLDEDRHLSFLAISSNLAPFFGLLGTVWGIMSAFFEIGQHGS
                     ADLSVVAPGIAAALVTTVAGLVVAIPASAGYNVFVARNGQNEIAYYNFGSRVLSIFKR
                     GDLLALEEIAG"
     gene            complement(13839..14549)
                     /locus_tag="PLT31_01790"
     CDS             complement(13839..14549)
                     /locus_tag="PLT31_01790"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPW93896.1"
                     /translation="MKYSLPTFLNDISSPIEGQRELSLHFQNKVIIGIDEVGRGPLAG
                     PVVACAVVLKNYEVDLGLNDSKKLSKSKRESLFDHIKEQCLCYAIGSASEAEIDELNI
                     LNANFLAMRRALSAIGVSGLDVTSSKIPVESKGFLTKETDWVIAIDGNLKIAGVPFEK
                     QIPIIKGDSRIVSISAASILAKVFRDRYMEDLAKKYPGYGFEMHAGYGTKKHLEAIRL
                     LGFSDVHRKSFHPKSLSV"
     gene            complement(14555..15145)
                     /gene="purN"
                     /locus_tag="PLT31_01795"
     CDS             complement(14555..15145)
                     /gene="purN"
                     /locus_tag="PLT31_01795"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HPW93897.1"
                     /translation="MFNFGVMASGNGSNFKALLSQIESKRLKAVCSFLIVNNKNCGAA
                     QIAKSNGIPVYHISSVTHPDSREYDQAFLNVIKQHPIDFLVLAGYMKKITDSLLVSLK
                     DRILNIHPSLLPKYGGHGFFGIHVHEAVLEANEGWSGATVHLVNGDYDDGRILGQSMV
                     PVFTDDTPEILAARVLKAEHNLYWKTLRDYAASLGY"
     gene            15423..15752
                     /locus_tag="PLT31_01800"
     CDS             15423..15752
                     /locus_tag="PLT31_01800"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPW93898.1"
                     /translation="MKTIYVGNLPFSFGDSDLSNLFEGFGEVKSAKIIMDRDTGRSKG
                     FGFVDMDDDGAAMNAIEQTNGQEINGRAIRVNESQPREARPPRFGGGGGNRGFGGGDN
                     RRQRRSY"
     gene            15857..>17290
                     /locus_tag="PLT31_01805"
     CDS             15857..>17290
                     /locus_tag="PLT31_01805"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl-hydrolase"
                     /protein_id="HPW93899.1"
                     /translation="MKFADYLKNAKMNGSVKDLMTPGLAVEFIQPWYTPLSTTPSSTG
                     IAVGGIGSTFTVTPAGTTPVMNVVPGVQVRANHPEDLRLNNFFFRESVVGKANASLEI
                     KNFSSFQRRNAFYTLTDAKGSPLFIETSCEEATAKLNRVIKDKKFFSSNEAAFSRFQI
                     EWSDRTTALIKKGDTTSPAFNRSVLIDFFDGVVGEEVSKSGALTAAWENDNSFLGMEG
                     YDPSKMNYSALYPVSHTEYKNQGLSIQRSQFSYVLPNEERLSSLPVSGTNFTIQNPTA
                     QTREITIVQIQDCLCGYLVQKDRQGIQDSSFVLTPAAKNPKGVQFDFNATDGRQVRGV
                     EFYNQNRLSESDFDGCMGISVSWDTKDQLNVSTKPVFYQNDAPKILEGALQSGRVSKT
                     FLKNVYSGRETIACAVVVTAVLKPKESVSFQFNTVLDFPFIRLNGLESQKKYTVFYPE
                     AFGRVQAILREALSADARISSELDKSFS"
BASE COUNT         5422 a         3521 c         3081 g         5266 t
ORIGIN      
        1 tcttcaccaa taagagtagt gacacgttcg tctgcaaggg ctttttcttc aagagctgcc
       61 atagcaggat agtgatctgt cttgaccatt ggtgtgtgaa gagtattata aagttcagta
      121 taaatgactt ctttaatagt agcagcaagc ttttcgtgtg cttcgctaac gtccttaggt
      181 ggaagaacca tcttttcttt ggcgcaacga tccactagtt cttgttgagc tttacagaga
      241 atcttaatgg catcatgacc agctaaaatt gcattaatca tggtgtcttc agaaacttca
      301 taagcaccgc cttcaaccat gcaaacagag tttgcggttc cagcgaccac taaatctaaa
      361 tcagaaatag cgacttcttc ataactaggg caaacgatcg gattaccgtc aactacggct
      421 actcgaacgg ctgccacttg ttcttcaaaa ggtaactctg ataaaccaat agcgagagaa
      481 gctgcactca cgccaagtac atcaggggca aactttttat cagcagaaag tgcctgaacg
      541 atgacctgga cttctcgaga aaagttttca gggaacatcg gacgaattgg gcggtcgata
      601 attcgagcag aaagggtttc ttcatcacta ggacgaccac cctcgcgctt tgaataacca
      661 ccaggtaaac gacccgctgc ataagctttt tcacggtatt ccactgtgag cggaaagaaa
      721 tccgcttctt tatcaggacc aaaacaaaca gtagcaagca ataaggcatc acccatctga
      781 acgacggcag agcctgcagc ctgtttagcc aaacgacctg tttctaaacg aatcgtacgg
      841 ccatcaggaa gagtggcaac tacttctttt ggatccaata ttttcttgta ttgtgacatt
      901 acttctccca gcacaggtta accgcgcaat ccgagttcct taacgagagc acgatagcct
      961 tcaagatctt gcttagaata gaatttaagc aaattcttgc gcttagcaac catcgctgtt
     1021 aaaccgcgta aagaatggaa atctttacga tgcattttag catgttctgt taaatttttg
     1081 attttttctg taagaattgc gatttgaaca cgtgcagagc cagtatcttt tgcgtttgca
     1141 ccgaacgctg ctgtgatttc ttgagatttt tcagttgtaa tggtagccat ttgctgtcca
     1201 tttccttttt ggttatttgt ttaaacaatt attaacgctt ccgtacttgc cgcacatcag
     1261 tggcgaactg atctgcaaga actttggcaa aattgcaact actggggata aaatttagtt
     1321 tttttcattt tactttcatt actctttttt tcaaaaagtc attttatcga cctttttttc
     1381 taaatttgcg gccatgattg acaaactgaa cctctctttt gggttttatg attaccttct
     1441 gattttcgct gtggccgcat tagggacttt tagtgcctat ttaaaagacc cacaactgaa
     1501 ggccgtctgt gcgacaattc ctattccttt tggatttgcc tacctcgccc tagagatgcc
     1561 aatcggagca gccaatgcag gttcagcctt attatgtctt ctttttgttc atattgttcg
     1621 ttacttgact tacaaaaaga atttccatgt gattctttcg atcattttag gaatttgtgt
     1681 ctttattttt attggcgcca tgcttttacc gtatttaccg actcaggaat ggttctttct
     1741 attgatttgc gctttagatt ttggtctagg tcttttaatt tttcaaaagc aaagttacag
     1801 acacggtgtt cgacataaaa cgcctctccc tgtctatatt aaggcacccg ctgtcgctgc
     1861 tgttgtggcg atgctaatgg tcatcaagaa aatacttgga ggctttgcaa ctagcttccc
     1921 tatgatgaat tcgattgtca gttatgaatc tagacactct cttggagatc aatgtaggca
     1981 gctccctcta tttttgatag caggcccgtt tatgtttatc gtaatgcgct atacagaact
     2041 tctcttgcat ttcaatcgtt ggatagttct cgccatcggg gttacgttat atatggcgtt
     2101 attctatcct ctaaataaag agctcaaacg acgtaatgaa agatcgtctc gagcttagtt
     2161 tgaagctagt tttaaaaaca tcaataaatt aaaatacagc tcgcgaagcg cgtaaaaaaa
     2221 caaacgacgc caaaaaatgc acttcgtaga atttattcaa aaaataacga tgtaaaataa
     2281 ttatagcgaa gttagcttga aaaataaatg atgtaaaaaa aatgctcgcg aagtgtgctt
     2341 aaaaaattaa acaacgcaaa aaaaacacga tttaaaaagt ggcaaaaatc attccaaaaa
     2401 gagccatgat gaaatgagct ctcaaggtta gttcaacgct agtttgaaat agcaacttct
     2461 gaaacggcta acgcaggcac tttcacagaa gaaaatgaat cattataggt gttccctaaa
     2521 gccacaagac gctttagtaa gtcaaagaaa ttgatactta gcgttaagcc ttctacagca
     2581 tgaacaggtt ttccattttc aacccaaatg ccttgaacac caatactcat ttctccactc
     2641 actgcactac agcctgaatt tccttcgagt tttacgatgt aaagacactt aggaaataag
     2701 tttaataagg cttcgtgaga ataagttcca ggagcgacga cgatattttt aaaagaggtt
     2761 tcaacttttc cattataaga acgagaggcg tttcctgaac ttaaaactcg atcacgagaa
     2821 gcggactcta aattataaag tttgttagaa aatacgcccg attgcaccac ctctattttt
     2881 tgagtgggaa caccttcgcc atcaaaagaa caagaccctg gaagatcggt tctccatgga
     2941 tcactgctta agttaaagaa gtcaggagca attttttcat gatacaagtc ttgtaaacgt
     3001 gattggcctt tctgaatcgt atcggcataa aaagaagaag agaacatgga aataatagag
     3061 cctgagacgc gattcgaaaa aacaactgga tgattccctt tttgaatcgg agatgggctt
     3121 aataactcaa gagctcgttc cacagattga gaagcaataa aagaagggtc aaattgagac
     3181 cagtccatgc ctgattttga gtagatgccg acttttttaa tgccatctcg actagcaaca
     3241 ctaccaacgc ctacagaaat gctatttcga tgacgttcat aaaaaacgcc tttgtgattg
     3301 gcaatccaaa tcgcgctttc acttcgatcg gccccaagat acggcacatt ttcaatttca
     3361 gaagatgcct taaaaacact cgcttctaca tctaaacaaa aagaagtcat ctccgataat
     3421 gaaatttttc ctaattctgg attatataat ccatcaatat cggtaagtgt ttctggagaa
     3481 ggtaaatcaa tgggtagtgc ttctgtaaaa gccgtatgtg ctaaagcatc ttcaagagtt
     3541 tgagagatcg ctgctggagt taaacgttct gtatgggcat agcctggaga accattctta
     3601 aaaacacgta taccaaggcc cactgactct gatatttctg tattttgcac cttgctctga
     3661 aataacgaaa ggccttctga aagggaacga cctgcaataa tatcaaattg ttcaactttt
     3721 ttagaggcta tttctttaag gtgtaagaca gcttcttgaa tatccataaa tctattttcc
     3781 tatgatagaa aagacttcag aagccttctc tataatttga ttaagaatga tatcgttatg
     3841 cattgtggaa ataaatgcgg actcatattg agatgggggt aagtatatgc ctctttctaa
     3901 catgccatgg aaaaaacgtg caaatgtaga tgcatttgct gccatcgctg tttctgtatc
     3961 gatcactggc ttatctgaaa agaaaacggt gatcatagag cctatttgat tcacctgcac
     4021 aggaacgcga gcggtggcgg cggcatcgcg caaaccttga gcgaggcgcg atccttgcag
     4081 agcaagctct gcccagggct tttcttgttc taaaaggtct aattgagcaa gccctgccgc
     4141 cgtcgccatg ggattccctg aaagagttcc agcttggtaa acgcctccga gagggcttac
     4201 catttccata atttctcgac gcccagcata agctgctaaa ggtaaaccgc caccgacaat
     4261 ttttcctaaa gtcatcatgt cgggttttac cgcaaataat tcacttgcac caccacgcgc
     4321 cagacgaaaa ccagtcatca cctcatcaaa tattaaaacc gctccagatg cgtcacacaa
     4381 gcgtctaaga ccttccaaaa atcctttagc aggagtcacc acgcccatat ttccagcgac
     4441 tggttctaca atcacagcgg caatttcatg agagtgtttt tcaaataaaa cttctacttc
     4501 ttcgaggtga ttataagaag cgactaaagt atctttagca acaccttgtg taacgccaag
     4561 agaacttgga acgccgtgag ttaaggctcc tgaacccgct tgtattaaaa aagaatcgcc
     4621 atggccatga taacagcctt tgaatttcac tattttatct cgccccgtaa atccacgagc
     4681 cacacgcaca gcagacatcg ttgcttctgt gcctgaattc actaaacgaa ctaagtccat
     4741 cccttgaacc atcgataaaa tgcgatcggc taaaagaact tcagcacctg tacaagcgcc
     4801 aaaactaagc ccttttttta attgtttttc taatgcttct gttaccacag atggattatg
     4861 acccaaaatc attggcccaa aagaaagcac taaatcgata tattcacgat cttcaatgtc
     4921 ataaataaaa gggccttgtg ctctagaaat aaaaatagga gttccaccga ctcccccaaa
     4981 agcacgcaca ggagaattta cacctcctgg gattttttca caggccttta accataaatc
     5041 cgtctgactc ataaacttct ccggtatttg gattcaagca ttttccagaa attagatggg
     5101 cttccagtca atgcttcgag acgttccgat aaagaaggag tgatttgttc ttcgcctgcg
     5161 attaatcgat ttaaaaaaga ttcatctact tctagacgac gtgcaaaatc actacggctc
     5221 atttttaaga aatgaatgct ttcttctatg gtgactccag gaggctgtat ttttcttcta
     5281 ttagtcatat tccctttaca atgaaatccg tttccatcct tcagcaccaa aacgcaaatc
     5341 acgttccaca aattcccaaa tcactaattt tttgcctttt aatacaccgc ttcgacgagc
     5401 aagcgtttca cgtacaagag tacttgcacc tccatcactc actatagaag aaagaggcgt
     5461 atttaactct tttgctaaat gagaaataaa gcccgcactt cgaggagcat cggtctggta
     5521 tattcttgaa aaactatctc ctaaaatcaa aatagaactt tgcctataat tatccttgta
     5581 tagaacacca gaagagtctt tcactggata agcttttacc cattgagaag gaaatacagg
     5641 taaagaagtc atcacagcca catctccgac tcgttcaact aatgtggtat cttctttata
     5701 ataattaaca ggtaaatcca tctccatcga acgaaccttt tcagccacaa cagaagcggc
     5761 aagcttagct cctcttggag tccaatgcgt gtctgtgtac aaatacaaat aattagaatc
     5821 ttgatcttct ttttttgctt ctaaaaatgg tttgtaaaga tcgactgttt taatgccttt
     5881 ttcatttaat gcatttaaga catcttgtga atgtgtaaaa ggaacacctg aagatgaatc
     5941 ccaagaggag gttaaaagct ctgggtaaat agaaggcttt cctggtatga tcacgactaa
     6001 aagttcaatt cctttctttt gtaaatcttt ttgaaaagca acaatttctt ttactgggtt
     6061 atctaagtgc ttacttctat ttttatctaa tgggcttggc attgttaaat aggcgacatc
     6121 gtcacgatag aaaagccaat cgtcttctcc taaaaaagct ttttctccag gctcattaaa
     6181 taaagtccaa aaagatcttt gtattaatac gcgacttttt tgtgtgactt tattttcatc
     6241 ttctaaacgt ttttcatagg gacgccaata acgacttgtt aagaaaccat aatgccagta
     6301 atactcagct aaacgtttga tagaaaaaga gccataggat agactcgctt gtttaattaa
     6361 aggtgttaaa gaatcagata cggaaggatt atcttctaat tcacttaaaa gcgattctat
     6421 tttcaataga tcctgatctt ttatggaaga ggaatccatt gtagtataag aattcgtatt
     6481 cgtataagat gtttttaaga aacgaaaggc atctaaaacg ggttcaagcg ataatgcatc
     6541 aaattctttt ttatttaaca cggattgcca agttttattc aaagaatcac tcgcctgacg
     6601 aagatcagac tctctattaa aaggagtctt aaaaatatcc gtgatcatgt ctagtggttg
     6661 aaaagcaaca tctttattca atgaataaaa taattgcgta gatgctggaa cacataatat
     6721 gccaacaaaa agaataatga agaaaattgc aatacggttc ataaaataaa tatagtaagc
     6781 tcaagataaa aaaaatccct attcccttgc tttggatgtt tttaatagat tacaaagcgt
     6841 tcaaaaatca ttgattttac gtaaaataat aactagtggt ttttagggct taaaatcatt
     6901 ttttaaagtt gcctcttttt cttttttttg ttataaatgg catcaaccac aaggagacga
     6961 acgtgagtat tcaagatgat ttcggcagct tagtctttaa tgcaaagaca atgagtaagt
     7021 atttatcgag tcaaacctac caaagtcttt tagcaaccat gcataatgga actgatttag
     7081 atccatcgat tgcagatgaa gtggcttatg gcatgaagaa ctgggcctta gatcaaggtg
     7141 caacccattt tacacattgg tttcagccat taaatggaac aaccgctgaa aagcacgatt
     7201 ccttttttaa tccagactat gaaggtggcg tcatcgcttc tttttctggt aaagaattat
     7261 cacagagtga accagatgca tccagttttc cttcgggtgg tctaagagcg actttcgaag
     7321 cccgtggtta tactgcttgg gatcctacta gtcctgcttt tattcgtaag agtcaaggta
     7381 atgtttcagt cctttgtatt ccgtctgttt tctgcggata ccatggcgaa gctcttgata
     7441 aaaaaacgcc tcttcttcgt tctcaagcgg ccattattaa acaagtttat cgtgtcgcaa
     7501 aactatttaa tcttccaatg gataaacgtc catatattac tttaggtgcc gaacaagaat
     7561 attttttaat tgatgaagca gtgtacaaaa tgcgtcctga tttaatgcaa acaggccgta
     7621 ctctttttgg tattcgtcca gcgcgtcatc aacagatgga agaccattac tttggaaaaa
     7681 tcaatcctcg cgttttgaat tttatggccg atgtggatca tgaactttgg aagctaggga
     7741 ttcctgcaaa gacaagacat aatgaagtga gcccagctca gtttgaattt gctcctgtct
     7801 ttgaagaaca aaatctcgcc gtagaccata atatgctcac aatgagtgtt ttacaatcga
     7861 ttgctgaacg tcacggattt atttgcatta ctcatgaaaa gccctttgct ggtgtgaatg
     7921 gctctggaaa acataacaac tggtctatca ctggaccaga tggtaaaaac tggttgactc
     7981 caggtaaaac acctcatgaa aatgcaaaat tcctttttgt tctagtcgca attatcaaag
     8041 cggtagatac ctatgctcct ttaattagag cggctgtcgc ttctgctggt aacgaccatc
     8101 gacttggtgc aaacgaagct cctcctgcaa ttgtatccat tttcctcggt gaacagctca
     8161 cagatattat caatcaatta gaatgcggta ctcctaaatc ctctaaatgt ggagggcttt
     8221 tagaaatagg tgtttcaagt ttaccaaaac tacctaaaga cgctacagat cgtaatcgca
     8281 caagtccatt tgctttcaca ggaaacaaat ttgagttgcg agctgttggt tctgatcaaa
     8341 gttgtgcctc ttctaatacc gttctcaact tgtctgtagc aagtacttta gacgaaatga
     8401 ttacagaaat ggaaaaagcc attacagcgg gtgaagattt caacatcgca cttcaaaata
     8461 ttcttcaaaa agaaattaag gctcacaagc gtatcttatt tgatggtaat ggttatagcg
     8521 atgaatggcg tgatgaagca gctcgtcgtg gtttacctaa cttaaaaagc actccagatg
     8581 caattcaagc ctttaaagat ccaaagttca ttgctcttta tgaaaagtat tctgtcttat
     8641 ctgaaaaaga acttaatagt agatacgcta ttgctaaaga gacttatgag cgagtcatta
     8701 ctatggaagc tgaatgtgct ttgatgattg gaaagacaat gattttacct gtcggtatct
     8761 cctatgccgc cgacttagcc aattcattat catcgctttc aaaagtagaa catagtgcct
     8821 tgcaaaagat ggtagaagca acagcctctt tagtcaattc tttgattgaa gcaatagagc
     8881 acctagaaac ttctatcaaa agtggtcatg cagatcagat gcttaccgca atggataaaa
     8941 cacgtcagtt tgctgataat ttagaagaaa ttgttcctga taatcaatgg ccattaccaa
     9001 gttatggaga catgtttttc atctaatttt ttaattaact aaactaaaat agcggctatt
     9061 catagccgct atttttttta taatgaaaag ctaaaaaaac tttgcatagt agaatcctta
     9121 atattttaga aaaacaaact gatgcaaaga tttatagaaa atgttatttc ttttcttgat
     9181 ttaataattc ttcaagaatg gctgcccctt gtaaaaattc attagactcc atacagctct
     9241 ttacttctag cagtttttgc aaatattgct tttgtgaagg aatattattc tcttctgcag
     9301 ccatgttaga gagcttaaat aaaaccgtac aaatctttgt actattaggg aattgttcta
     9361 caaagcccga aaataatatt cgtgctttat ctttttgatc cacttgaact agacaaagag
     9421 ccatccaata aagagattct tctgcaaatt cacctgtttt catcaactca tagacttgct
     9481 taaaagttga atacgcaagc ttgtattctc ctcttaaata atcagagcga cctgtattaa
     9541 acatggattc tgcttctaaa agccgttcag cctcttgttc aatactatct aaagcaagag
     9601 gctctgatga agacttatta atcaccactt ttttagcaag aattttgtct gatttgccta
     9661 aaagcatatc taaacgataa agaatttctt cttgtcggct atcatttcgt tctgattctg
     9721 atgccactcg ttttgataaa atagaaagct ctgccattag gcgacgattg actagatctt
     9781 gagctttata aagagaatca atcacttgtt ttaaagaatc cacttgtaga ttcatttcat
     9841 ctttaacatc ctttctaaca acgtttccaa caactcttaa ttctttggtt ctaagtgcag
     9901 tcacactaga acaccccgac ataaaaatta tcgaaaataa aacgaaaaca agaaggaatt
     9961 tcagtttcat aaaagatctc ctcaaggagt tcaaaacaag ttattatttg acattcacac
    10021 ggaaattagc tctacgattt tttgcataag catcttcact ttcaccatct tgttttggtt
    10081 tttcttcacc gtaactaatg gtttccatac gttctgaagc aatgccataa gcgcttaaaa
    10141 attcttttac ttttgtggaa cgagcactac ctaaagtcat attatagtct tcagtgccac
    10201 gagcatctgt atgaccttct acgataatag taaaacgagg ttcttggtta agaatttcac
    10261 ccacttgagt gagaagttgt ttagcatcct ctgttaattc agaacgatca aaatcaaaat
    10321 agatgtcttc agacatgacg cggtccatca tttcttgtaa ttttttacga gccgcttcta
    10381 aacgctctgc ttctaaacgt tctctttcaa gtgcagctaa agaatcttca tttacttttg
    10441 attgctctgg agccactgct tcagcaggtg tttccttgac tactggatct acaacaggtt
    10501 cctttttttt agaacatgcc gttaatccaa taacgacgca acctgcaatt aaaagagcat
    10561 aaattgattt cattctttac cttccttttg ttttgtttga ttttcaattg gataccaaga
    10621 ccaagtaggt gctgtattat ctccaccctg agtaattctc gttacattac ttccatctcg
    10681 gcgcataata taaatttgat tagatccaga tctattactt gaaaaagcaa tcaacatgcc
    10741 atctggagac cagcttggtc tttcgttatt tccagaattg gatgtcaatt gaataatatc
    10801 tgacccatca atagcacatg tataaatatt catttttcca tcgtccatag atgtataaac
    10861 gatccggtcg cccgtaggag cccaagaagc cctttcatta taccttccca tgaatgtcat
    10921 ccgacgcata tcagagccat cactattcat gacgtaaatt tgtggagaac cacctcgatc
    10981 acttgtaaaa ataatttctg ataaattagg actccaagaa ggacttgttt gagaagactt
    11041 taagaaagaa atacgacgag ttttttgtgt ttctggatca cccacatata aatccgtttt
    11101 tccatctata atggcagaaa aaagaatttc gcctgttttt ggattaactg cagggctaaa
    11161 agtttgctca aatttcttaa ataatctttt ttgtgtccca gtagccacat ccttaatata
    11221 gagctgcggc ttattatttc taaaactaac aaatattatt ctttcattat caggactcca
    11281 tgcaggcatc atactaatgg tagaatcaaa ggtaagctga cggcgatgga agccatcata
    11341 atcagcaacc ataatctgtt ttacaccatc tattttagaa acccaagcaa gtttagtaga
    11401 ggcaatgcca tggacacccc acaagttata aattacttta tctataaaat catggacggc
    11461 ttttcttgaa tctattaaag agacggtgta agactcacca aggactaaat cctttgattg
    11521 cgtcgcataa aggtaaaaat caatctttat tttattattt aaggcctttt caattttacc
    11581 agtcacataa aacttggctc ttgcccgact taacttcact aaatcaaatt tcggaactga
    11641 aattacatca aaacgcccag aaagattaag atctcgttca gcgacttcgt gtggtttttc
    11701 atctggccaa tctaaagtag ttccagatgg ttctgagaaa ggaacaacac ctataggcat
    11761 tgttttgaaa acagatatac caacatcaac ggcaatagtg tctattgaag caaacacctg
    11821 cattgtcatt gataagaaaa gagctaaaaa taatgattta ttcatacaca agccagaaga
    11881 tacaaattaa taaagacaaa aacaaaaaat aaattacttt ggtgtgaaat taaaatttaa
    11941 cgaaagcgct ggagccctat agtttggtgg cagctcgggc actttagcaa tttgaatagc
    12001 acgcatagaa agatgatccc acgttttatt tccagaagag cgtttgagag taatatttga
    12061 aatcaaacca aaacgatcca cagtaaactg gacggttgtt ttagcgtctt tcccaatatt
    12121 catcccatta ggagggttga aattttgcat gataatttgt tttaggcgtt ctaaataaac
    12181 ctgcatcaaa ggatccatat ccacagagcc aactggatga agacttggag catccatcgt
    12241 aggcaaatcc atgtcatcta taggaaaatc atcggtcact tcttcggtgg tttcttccac
    12301 aacttcttca actggttcag gctttggttc ttcaggttta ggttcttctt gtttaggctc
    12361 tggcggtagt tcttttttta cttttggttt aggtggttct acctttggtt ttgggcgatg
    12421 aaccacctga cgaggaacag gggctgccac ttgaaccatt tcaaacacgg gaataggttc
    12481 tggttcagat ttaaaattaa tgttatttac aataaataaa gctgctatac ataacaagtg
    12541 aaataccaaa gacaccacaa caatttttgt tatggaacta ttgatgccag acgaaaaata
    12601 aagcgatcct ttatcggtca ttttttaaat tttctttagg cgataatgtt aaaaagccta
    12661 gtttagtaac ccccgctttt tgtacggcag agactacttt catcactaaa ccataattca
    12721 ctgtttcatc cgcattaatg acaacagcca tttctccatt ccaaagagac ttgaatactc
    12781 cattaaagct tttcatatcg actttcatat cagcgatata aagatcatca tctttagtga
    12841 tggatacttt aagaagtttt tcttgctcta ttgtaggcgc ttctgcctta gggagatcga
    12901 ctttaacgcc ttgactcatc aaaggggcag aaatcataaa tacaattaat atcgaaaaaa
    12961 cgacatcaat catattggtg agattcattt cttggtttaa tgttttaccg cgacttcttt
    13021 tcaaagtgcc tcctacccag cgatctcttc aagagcaaga agatcgcctc gcttaaagat
    13081 acttagaact cgagacccaa aattataata ggcaatttca ttttgtccat tacgagccac
    13141 aaagacgtta tagccagctg aagcaggaat tgcaacaaca agaccagcaa cagtagtcac
    13201 caaagccgca gcaataccag gagctaccac tgaaagatct gcagagccgt gctgaccgat
    13261 ttcaaagaaa gcagacataa taccccaaac cgtacctaag agtccaaaaa atggggccag
    13321 attcgagctg atagctaaaa aacttaaatg acggtcttcg tcaagacgca agccttcaat
    13381 cgaacgttga atagtatctt caagcaatga ggcgcgatgt tgaatagaat cataactcac
    13441 aaaattactg aatttagcag cttcaataag tacttctgat gtcagtcgtt ttaacgcact
    13501 atctccagaa gtttcacata agccttgtaa ttcaataaaa tgttcgacct gattaaacct
    13561 tgaaaaaaaa gaagcgttag ctctttgatt ctttcgataa gaaaataatt tgacaataat
    13621 aatgccccaa cttccaaaag acattacagc tagaatagca attatcacaa ttgtagccac
    13681 atcagctttc ataatcatct gaactatagg taagctaagc acaaaaacct ccataaaaga
    13741 caaagtaaaa atagattttt tttcttttta aaagaacgac tctttgctta aaactgctgt
    13801 tattgattga aatgacgctt gattctgcat gaaaaaaact agacgcttaa gctctttggg
    13861 tgaaagcttt ttcgatgtac gtcactaaag cctaacagtc gaatagcctc taaatgcttc
    13921 ttagtgccat accctgcatg catttcaaaa ccatagccag ggtacttttt tgccaagtct
    13981 tccatataac gatcacgaaa gaccttagca agaatagaag ccgcagaaat actcacaatt
    14041 ctagaatccc ctttgattat cgggatttgc ttttcaaaag gaacacctgc gatcttcaaa
    14101 ttgccatcaa tagcaatgac ccaatcggtc tcttttgtta aaaagccttt cgattctaca
    14161 gggattttcg aggaagtgac atctaaacca gaaacaccta tcgcagataa cgctcgacgc
    14221 atggcaagaa aattagcatt gagaatgttt aattcatcaa tttctgcttc actcgcgctc
    14281 cctatggcat aacaaaggca ttgttcttta atatgatcaa acaaagattc tcttttactt
    14341 ttagataact ttttagagtc atttaagcca agatctactt cgtaattttt aagaacaacc
    14401 gcacaagcaa ccactggacc agctaaaggg ccacggccta cttcatctat cccaataatc
    14461 accttatttt gaaaatgtaa agaaagctct ctttgccctt ctataggtga agaaatatca
    14521 ttcaaaaaag tgggcaaaga atacttcata ttttttaata ccctaaagag gctgcataat
    14581 cacgaagagt tttccaatac agattatgtt ccgcttttaa aactctagcg gctaaaatct
    14641 caggagtatc atcagtaaaa acaggcacca tagattgccc taaaatacgg ccatcatcat
    14701 aatcgccatt cactaaatga actgtcgccc cactccagcc ctcattagcc tctaacacgg
    14761 cttcgtggac atgtattcca aaaaaaccat gcccaccata tttggggagt aaagaaggat
    14821 gaatatttaa aattcgatct tttaagctta ctaataaaga atctgttatt tttttcatat
    14881 aaccagcaag cactaaaaaa tcaatcggat gctgttttat aacatttaaa aaagcctgat
    14941 catattcacg cgagtctggg tgagtgacgc ttgatatatg gtatacaggt atcccgtttg
    15001 acttcgctat ttgagcggca ccacaattct tgttgtttac gattaaaaaa gagcaaaccg
    15061 cttttaaacg cttagactca atctgagaaa gcaatgcttt aaagttactt ccattaccag
    15121 aagccataac accaaaatta aacacaggtc aaatataata agatttaaat ttttgatagc
    15181 ttaacatttt tttctttatt ttttctctgt ttataataag gtaaactttt agtctcatgt
    15241 tttttttgcc agaaagaggg cgtttttaaa aactgggata atgaggcccc ctcaaaaaac
    15301 ggaaaaaaag gccttttcaa aaaaagaagg taaaaagaat tcatttttta caaaaagatt
    15361 caccgttttc cattttttat tctacaattg tactcagctt aaacaaacaa gcatggatta
    15421 atatgaaaac catttatgta ggtaacctcc ccttttcgtt cggcgatagc gacttatcaa
    15481 accttttcga aggctttggt gaagtaaaga gcgcaaagat tatcatggat cgcgacactg
    15541 gtcgcagcaa gggcttcggc tttgtagaca tggacgatga cggtgcagca atgaacgcca
    15601 ttgaacaaac aaacggtcaa gaaatcaatg gccgggcgat ccgcgtcaat gaatcacaac
    15661 caagagaagc acgtcctcca cgttttggcg gcggcggcgg caatagaggc ttcggcggtg
    15721 gagacaatcg gagacaacgc cgttcttact aagccgattt aatatttcaa acgcattgga
    15781 gcttctagtt aggagctcct tgttttttta aattaacttt accttttagt tctacattat
    15841 ttatcatagg tgaaaaatga aatttgccga ctatcttaag aatgcaaaaa tgaatggctc
    15901 tgtaaaagat ctaatgactc caggccttgc agttgaattt atccaacctt ggtatacccc
    15961 actctctaca acaccctctt ctacaggcat tgctgtgggt ggtatcggaa gcacattcac
    16021 tgtaactcca gcaggtacaa ctcctgtgat gaacgtcgtt cccggagttc aagttcgagc
    16081 taatcatcca gaagatcttc gattaaacaa tttctttttt agagagtctg ttgttggcaa
    16141 agcaaatgca tccttagaga ttaaaaattt ttcatctttt caacgtcgaa atgcttttta
    16201 cactctaact gacgccaaag gatctcccct ctttatagag acttcttgtg aagaagctac
    16261 ggcaaaatta aatcgcgtaa ttaaagacaa aaaatttttt agttcaaatg aagctgcctt
    16321 ttctcgcttt caaattgaat ggagtgatcg tacaacagcc ttgattaaga aaggcgatac
    16381 aacttcccct gcttttaacc gttccgtttt aattgatttt tttgatggcg ttgttggtga
    16441 agaagtttca aaatctggag cactcactgc tgcttgggaa aatgacaact cgtttcttgg
    16501 tatggaaggt tacgaccctt caaagatgaa ctattcggct ctctatcctg taagccatac
    16561 agaatataag aatcaaggcc tttctattca acgttctcaa ttcagttatg ttttaccaaa
    16621 cgaagaaagg ctttctagtt tacctgtttc aggtacaaat tttacgattc aaaatcctac
    16681 cgctcaaact cgtgaaatta caattgttca aatacaagat tgtctttgtg gttatttagt
    16741 acaaaaagat cgccaaggaa ttcaggactc ttcttttgta ctcactcctg ccgcaaaaaa
    16801 tcctaagggc gttcaatttg attttaatgc taccgatggt cgccaagtca gaggtgttga
    16861 gttttataat caaaaccgtc tttccgaaag tgattttgat ggttgcatgg gcatcagtgt
    16921 ctcttgggat actaaagatc aattaaatgt aagcacgaaa cctgtctttt atcaaaatga
    16981 tgctccaaaa attttagaag gcgctttgca aagcggtcgc gtttctaaga cctttctaaa
    17041 aaacgtttat agtggtcgcg aaaccattgc ctgcgcagtc gtagtgacgg ccgtcttaaa
    17101 gcctaaagaa tccgtttcat ttcaattcaa taccgtttta gattttcctt ttattcgctt
    17161 aaatggttta gaatcccaaa agaaatacac tgtattttat cctgaagctt ttggtcgtgt
    17221 tcaagcaatt cttagagaag ccttaagtgc agatgcaaga atttcttccg aattagataa
    17281 atcatttagc
//
LOCUS       DAOOBB010000019        17183 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_4323_length_17183_cov_5.221859, whole genome shotgun sequence.
ACCESSION   DAOOBB010000019 DAOOBB010000000
VERSION     DAOOBB010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 17183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17183
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4323_length_17183_cov_5.221859"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="PLT31_01810"
     CDS             complement(<1..934)
                     /locus_tag="PLT31_01810"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HPW93900.1"
                     /translation="MKAEELAQPHLLKQPVYVTGKPIAYTAREFGLDPKKIDKLASNE
                     NPYGPSPKGMAAAQKALSEINLYPDGGCYDLTTKISEFRGVKREQIIIGNGSNELLDM
                     IAQVFLGENTEAIMGNHSFAVYKLATLAMNAKPVEIDMPAPDYKHNLKAMKEAINERT
                     RLVFLANPNNPTGSEIDAQEILDFAKSLPEHVILVMDEAYTEFIESEAPNLLPLIEKG
                     LPIICCRTFSKIYGLAGLRVGYAISRPDIISLIQRVREPFNVNSVAQAAAIAAIDDTS
                     FIEEVREKNTQGLLQLETGLKAMGLSYVPSRANFI"
     gene            1441..2076
                     /locus_tag="PLT31_01815"
     CDS             1441..2076
                     /locus_tag="PLT31_01815"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HPW93901.1"
                     /translation="MENDHTKLLHGKKVAVLVETEFIPSEVAHYKKFFENLGAQVDFM
                     TYLWGEKERTLVSDVDSVGKEVQTLVVDREIAEANPNDYAFVLTAANYVAVRLREIPP
                     MGCLGSLETVRSPSAVRFMASAMCNPFIVKGALCHALWILTPNPELLKNRKVICHTVV
                     LADIINAGAQFVPDEKHVVIDHDLVTGRSAADIDSFCEALIQSYKTIHNIQ"
     gene            2087..3544
                     /locus_tag="PLT31_01820"
     CDS             2087..3544
                     /locus_tag="PLT31_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93902.1"
                     /translation="MKNITNTKLAVSTSDWLKHLDNLLLFWDKKEAYATGKSDLFPTY
                     RCNTGETFDINANPLPDEIVDALNSEDTKGLIELDKNFIRAHSRQTYAYGIAFHMTGK
                     GKYLNLCKKGVDALLKAIDGNYGMCVTQTLSGKWDNARSDRTSQDLAYGLTGLGMYYF
                     LTHDKTILHRIIQLKDYIFNAYLSDRRGYLMWLPKNNPKSEGEVQIVAQLDQLYAYML
                     MLTPCLPEPYKSVWKKDMKRIADILITHFYSEKYNMFWGTQNNMDRMQLGTDHTDFGH
                     SVKTFWVILKVGQLLGEPFYVHFARPNIEKIIKDAFIPRVTGSAIPGSWARRFDANNE
                     LDIDKEWWILAELDQAAEILSIHDPFFYDYLNETQKYWLEVMTDKKDGEIWHMVDGKT
                     NEPVHRFPKIHNWKTSLHSFEHVLFSYMTAANFANENFHLYYALPEWETADQDNVAPY
                     MFFANVVKVEKQKSDIQLELPDNNEVYKVTFNNLH"
     gene            complement(3729..6119)
                     /locus_tag="PLT31_01825"
     CDS             complement(3729..6119)
                     /locus_tag="PLT31_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan lyase"
                     /protein_id="HPW93903.1"
                     /translation="MKGLVVSAILALSLASTAGAVRQMEFLDRGLVGVKVDGGVFLSW
                     RLLGNDAATTSFNLYRDGTLVNASPITGATNYTDTKGSTSSLYEVRPIINGEEKGAEK
                     SVSVWSSQKLTINLDRPAGGSNSSGSYTYSPNDIAVGDLDGDGEYELVLKWDPSNSQD
                     NSKSGYTGNVYIDAYKLNGKKLWRLDLGKNIRAGAHYTQILVADYDLDGFAEVAMKTA
                     PGTKDGTGSYLSNGPAASVDHSKDYRNSKGYVLSGEEFLTIFNGQTGKEMATVNYNPA
                     RGTVSSWGDSYGNRVDRFLATNAYLDGNKPSMVFQRGYYTRMAVTAYDWDGKTLSQRW
                     YYNSPTKGQECYGQGNHNISAGDVDGDGYDEIIEGSCAIDHNGKFMYRTGLGHGDAMH
                     LGDLDPDREGLEVWQVHEDSPYGYDLHDARTGERLFYEKSSGDNGRGVAADVDSTSRG
                     SEMWSGANYNTYSVKGTILASSRPAYNFRVYWDGDLLDELLDNVTISKWNSSTKKNTT
                     LLTMEGASCNTTKATPNLSGDILGDWREEVILHDDSHLFLYTTTIPTTHRMYTLMHDP
                     VYRLGISWQNTAYNQPPHLGFWLASGNFPTPKIELVGEILDPLLVKNGAGSSKQTINL
                     GEPIVDFAYAYSHCTGAVATGLPAGITTSLANGKLNFTGTPTEAGVFGFTVTTTGGAL
                     DAYVKTGVITVIDPNAPADTSKDDSTSEDDLTGIRLAKIGSAFLTISREGIAYQVPQT
                     NAKLFIFDVNGNTVFERSVQFGGMISNEWNALPAGRYVITLRAGGKFLQKIPAEKR"
     gene            complement(6231..6620)
                     /locus_tag="PLT31_01830"
     CDS             complement(6231..6620)
                     /locus_tag="PLT31_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93904.1"
                     /translation="MNEFKSSCEFDINDSIWEYMYVESIGELNVIQHHFNEDQLDIKN
                     LHSIDLDKKSCDSLQKNGFTFITLFCLDTMSFVLDYISTVEKNLDGTYGKWHRSKKQI
                     LFVFTEPCVQSIGDNRKMKELFENTSK"
     gene            6886..7368
                     /locus_tag="PLT31_01835"
     CDS             6886..7368
                     /locus_tag="PLT31_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MraZ family transcriptional regulator"
                     /protein_id="HPW93905.1"
                     /translation="MNLDFFIGQAKTAIDEKGRTSFPREFRRQLSPEESESMVLSIGP
                     ERTLILYELNEFKKFMTELNARPRTRQTEALRSTIQGHTSFVSLDGQNRILLSKKFLE
                     YAKIESEVLFVPHVGKSLKLWNPDIYESSYGFSSPEDYEQFDAGFFSDVWMGDSSEKQ
                     "
     gene            7304..8293
                     /gene="rsmH"
                     /locus_tag="PLT31_01840"
     CDS             7304..8293
                     /gene="rsmH"
                     /locus_tag="PLT31_01840"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPW93906.1"
                     /translation="MNNLMLGSLAMFGWGTRVKNNRNYHDPVFLNETLKYLSISSEGV
                     FADCTLGGGGHSLAIVERLGPEGHLHCFDRDPEAIAFASNRLKAFEKQITFHPVPFSE
                     IENEIDADSLDGVLYDLGISSHQVDDSSRGFTFVGNQPLDLRMNQQGDESAQQWLRHI
                     SAEDLAKVFSQNADLDRSYKLAVRLIEKIQTISGDILPQDVREVVESVFYDKRRDLNS
                     ILARVYQAIRMEINQELQQIEKSLRGAVSCLKKGGRLVVISYHSVEDRCVKNTVSAFE
                     KDCICPEKLPVCRCGGNHRLLKKEIRKPLLPTSTEIDQNSRARSAKLRVYTKV"
     gene            8290..8601
                     /locus_tag="PLT31_01845"
     CDS             8290..8601
                     /locus_tag="PLT31_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93907.1"
                     /translation="MTGVLKSTVTELTTGKMILLVSLFLLMVLVLFVVPLWLQNRYIE
                     LSEKEIRFSNEVIELKTEIIRLELSNNKLTSFENLADFARDHGLDYNIVPVKLMSVGG
                     N"
     gene            8601..10475
                     /locus_tag="PLT31_01850"
     CDS             8601..10475
                     /locus_tag="PLT31_01850"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPW93908.1"
                     /translation="MYLDRLAWLVLIFASLWLVLVSRTFQIQVLNQETYKEHAQEYAL
                     RQMNLYAERGKIMDRQGIVFADNIKDKDNKHLEYSRIFLQGKMASQLIGKVGYNGRGA
                     MGLERQFDEKLRGNSGYLLSVQTAGRKIIHGLEKDYEEAIPGKSMVLTIDRDMQEIVE
                     NSLKDGVETFKATSASAIVVDPFSGEILALASYPTFDPNQKLQTSASRAFRNDLVSLS
                     FEPGSTFKVITAAAALEENAVNPKRKMSGENGRWVLPNGEVIRDTKDHGEMDMTEAMT
                     YSSNIVYAKIADSIGSETFYRFVRDFGFGTKTSEDLIGEESGYLKEPHTWSGRTLKTM
                     GFGHELTVTPIQMVMAFSAIANGGKLMQPMIIKEWRDENGNTLEKNNPVEIRRVVSEK
                     TAAHIREMLYQVVVNGTAKSVMSSKIPNVDFGGKTGTAEKYSQELKRYDRNSQVASFI
                     GFSPARDVRYVCLVLMDDPKTKTVGGLTAGPVFRKIMESIYFHPKLSPTPYNLVQVAQ
                     DKKCEIDFVGMSVENAKKLAANKGCSVQVEGNGSVIVSQKINGEKDAQVSLKSGDLNY
                     SKMPDLRGLSLKEALEAMSLIRMPVEFSGKGRVLEQFPEPSTMISKGQTCKLVLKEKV
                     "
     gene            10477..11889
                     /locus_tag="PLT31_01855"
     CDS             10477..11889
                     /locus_tag="PLT31_01855"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HPW93909.1"
                     /translation="MIFKELLQDFKITGLADDSRRVQEGYLFFCFPVKESLSFAKMAL
                     DQGARAIVAETEAPLEMKSKWIQVKNVKEARLEAAILFYGNPFEQLKVHAITGTNGKT
                     TSTFLMEAMLNAAGRKTALIGTICNKIGNEIIPSFLTTPGLLDLFAFARHALEAGCTD
                     LVMEASSHALDQGRVAGISFKTALFSNLTQDHLDYHLTMDLYFEAKKTLFTKYLSKEG
                     HAVINVDDAYGKMLFDSLDGNKMAVSRMKSKEAMIYPEGNVAQSERGMTLLVPAISNQ
                     PFETHLCGDFNVDNVLLVLGFAKALQLPEMAIRKALENVRVHGRFELVYNQNKKHVVV
                     DYAHTPDALQRVLQTARRLCQGKLAVVFGCGGDRDKTKRPLMGSIATKNADVVWVTSD
                     NPRTEKPEDIIQDILKGISKKEDLYVIENRAEAIQKACVSMNENDWLVIAGKGHENYQ
                     IIGQTKHHFDDSEEVQKAML"
     gene            11886..13286
                     /gene="murF"
                     /locus_tag="PLT31_01860"
     CDS             11886..13286
                     /gene="murF"
                     /locus_tag="PLT31_01860"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HPW93910.1"
                     /translation="MIMKLDLTIAEMCEILETTPVGLKTRELQKKVQVCMDSREAAKG
                     CVFWPIRGVRFDAHDFITQVEGKGALMSVVNEDYAGLKSIKAYAPVDDSSEALLKLAK
                     GYQRRFHLKKVAVTGSNGKTTTKEMLRAVLSTQFNTHATQGNYNNHIGVPMTLFQLKH
                     SHEAAIIEMGTSGPNEIKPLSLAAEPNIAVITNIGASHLEGLGSLENVFKEKLSIVSG
                     LKKNGLLVVNADDAFLSKVRSTKSYKVITFGLRRGVIKPTHLKWNENACASFDIGRTH
                     FELKVAGIHNLYNALAAIAVGVSLRIPKTQIAKALSSFRASSMRMEIHKANGFRVVAD
                     CYNANPSSTRMALETIGNISTHEGRRIAVLGDMLELGADSSKLHHQIGRLIPEMNFDF
                     LIAVGKDAKEFQKGAIEGGLSPEKALYFETTLEAMNFLSEEVRQNDILLIKGSRGMKM
                     EKIVEMLLRLEPVYGV"
     gene            13289..14434
                     /locus_tag="PLT31_01865"
     CDS             13289..14434
                     /locus_tag="PLT31_01865"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HPW93911.1"
                     /translation="MDRFLNMKFDKGLLLAVILLLVLGIVIIYTGTSPIAMQKGLAAE
                     YFTISHIKKVVLGLFMMCVALVLDYSVWYKLARPLFVICLLAMIAVLFQGDSVKGASR
                     WLSIAGMKIQPSEFMKLGIFCLLSVKLVEAGDQINDFKTGFFRPLILVGIVCVTLILQ
                     PNFSMVLMVLATSLILIYVAGVPMKHLMTLIIFPPILSIIAYTQSYRSLRIKAWLDPE
                     SYPEKAYQLRHSLIALGNGGFTGTGIGQGTQKLGYVPESYKDAAFSILGEELGFIGAF
                     LMLSLFAFLAYRGFAIARSSSTRFGKYLAVALTSSICLNMIFHVCVCTGLFPTTGQPL
                     PFISFGGTSLVMTLLSIGILLNISKPNTGQNIREIPINSAFYSTRYL"
     gene            14431..15510
                     /gene="murG"
                     /locus_tag="PLT31_01870"
     CDS             14431..15510
                     /gene="murG"
                     /locus_tag="PLT31_01870"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HPW93912.1"
                     /translation="MKQKRFLFVCGGTGGHVFPALAIAESLKKKGIQEIYFAGRANSM
                     EQRLVSPHWPFFEMTAEPLRRGSFVKNLALPFKLSKSYIHAKHLIEKINPDVIIATGG
                     YVSFPIVLAGGRLGKPVYIQEQNAIAGVANKYGARYASMIFVTSEDAVKAFQHPNVKV
                     MGNPVRELPAKETLARPAEYSKGKKAVFIVGGSQGAVGINNKIEESIGRITSRDDVSV
                     VWQAGAKNVDALHERLGILPNVTICSFLDNIYAYMAHADLIISRAGASTLAEILAFGK
                     PSILLPYPHATANHQEHNARAVEHSGACLVELDNDPNDLWNKVEELLASPSKLEQMSK
                     AAEKLGSPDAADKITEIILKAERCI"
     gene            15507..16889
                     /gene="murC"
                     /locus_tag="PLT31_01875"
     CDS             15507..16889
                     /gene="murC"
                     /locus_tag="PLT31_01875"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HPW93913.1"
                     /translation="MNFISRKRIQRLHFVGIGGAGMSGIAEVLHENDFIVTGSDTSDG
                     EAIQYLKKLGIRIDSIHEAKNVEEADLVVYSSAVQQDNPELVEARARRIPIIRRAEML
                     GELMRLKYTLAIAGTHGKTTTTSMVGAIWEKAELDPTVIVGGVVKGKGSGAKVGRGSY
                     LIAESDEFDRSFLSMMPSSAIITNIDEDHLDTYKNLEEIKNAFVEFANKVPFYGQVII
                     CLDDLNVQSILSRLTKPIITYGFSRQAIYRIEGLRREQENFVFDVFKNNELLGTFKIK
                     TPGKHNVLNATAAIALAVEEQIPLNVISEAIASFEGVHRRFEYMGEYKGALVYNDYAH
                     HPTEVAATLQGFRDAFPDRRLIVVFQPHLFSRTRDHHGFFGTAFGNCDILFGCDIYPA
                     REKPIEGVTGELVVQSALDRGHRDARFIGAKENALIALKKELNEKDIVVFMGAGNIWL
                     QGKELLEDKI"
     gene            16886..>17183
                     /locus_tag="PLT31_01880"
     CDS             16886..>17183
                     /locus_tag="PLT31_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /protein_id="HPW93914.1"
                     /translation="MTRDRTIFSRRRLGYNEVLRKERRQEKLKKSVGGSFKWFGQKGW
                     IAALVLVVLGVALWQGRYYLKDFNPTEWRSLKTIQINGNRMLTWEEIIQISRIEV"
BASE COUNT         5113 a         3008 c         3674 g         5388 t
ORIGIN      
        1 caataaagtt tgctcggcta gggacgtaag ataagcccat ggctttgagg cctgtttcta
       61 gttgtaagag accttgagta ttcttctcac gaacttcttc tataaaagaa gtgtcatcta
      121 ttgctgcaat agcggcagcc tgtgcaacac tattcacgtt aaaaggttca cgaacgcgtt
      181 gaatcaagct aatgatatct ggacgtgaaa tggcataacc tactcgaagc cctgcaagac
      241 cataaatttt agagaatgta cggcagcaga tgataggaag gcctttttca ataagaggaa
      301 gcaagttagg agcttctgat tcaataaatt ctgtataagc ttcatccatc actaagatta
      361 cgtgttctgg gaggctcttt gcaaaatcaa gaatttcctg agcgtctatt tcagaaccag
      421 taggattgtt tggattggcg agaaacacaa gccttgttct ttcgttaatt gcttctttca
      481 tggctttgag attatgctta taatcaggag caggcatatc aatttcaact ggctttgcgt
      541 tcatagcaag agtcgctaat ttataaacgg caaagctgtg atttcccata atggcttcgg
      601 tattttcacc aagaaaaact tgtgcaatca tatctaaaag ttcgttagag ccgtttccaa
      661 taatgatttg ttctctttta acgcctctga attcagaaat cttagtagtt aaatcgtagc
      721 aaccgccatc agggtagaga ttgatttcgg acaaagcttt ttgagctgca gccattccct
      781 taggacttgg tccataagga ttttcattag aggcgagttt atcaattttt ttagggtcaa
      841 ggccaaattc acgagcggta taggcaattg gttttcctgt gacataaaca ggttgtttta
      901 ataaatgagg ttgtgctaat tcttctgctt tcataacatc cttctttgta acacgctttt
      961 tttacaaagt caaatttaca aaaagcttct cttttggcat aggtttaatt actaattcat
     1021 cttgaattac ggtgaattaa aaaaagaatg ataagtttgc ttaaagaata taaggtgata
     1081 tgagataaac tcagtttcga gagtcttatt tagtaataaa atacaaagcg aattgtgaat
     1141 cattaaagtg aacgctgatt tttccgtttt ttcatcttgt tcggtagtgc tcgagatgct
     1201 tgctaaaaaa cttggttttc atatctacat agttactcag aatattattt tggcgatgat
     1261 tagtaaaaac gggttaatag aaaatgatta tgtatgatct atcgtgtgct ccctatacaa
     1321 gtaggtttag tgtttttgaa tgaaataaaa aaactttttg tgtttttggt aaataaagat
     1381 tatctatata atttacattg ttttttctga tttaaaatca aataggaagg ggagatgctc
     1441 atggaaaatg atcatacaaa actattgcat gggaaaaaag tagccgttct tgtagagact
     1501 gaatttattc ctagcgaagt agctcattat aaaaagtttt ttgaaaatct gggggctcaa
     1561 gtcgatttta tgacttactt atggggagaa aaagagagaa ctcttgtcag cgatgtggat
     1621 tcggtgggta aagaagttca aactctcgtt gtagatagag aaatagcgga ggcgaatcct
     1681 aatgattatg cttttgtact aacggctgca aattatgtgg cggtgcgtct tcgtgaaatt
     1741 ccacctatgg gatgtttggg atctcttgaa acagttcgtt ctccatctgc cgttagattt
     1801 atggcaagtg cgatgtgtaa tccgtttatt gtaaagggag cactttgtca tgcactttgg
     1861 attttaactc cgaatccaga attgctgaaa aatcgcaaag tcatttgcca tacggtggtt
     1921 cttgctgata ttatcaatgc aggagctcag tttgtacctg atgaaaaaca tgttgtgatt
     1981 gaccatgatc ttgttacagg gcgctcagca gcagatattg actctttttg cgaagcgctt
     2041 attcaatctt ataaaactat ccataatatt caataaggaa aatcaaatga aaaacataac
     2101 gaatactaaa ctagcagtgt ctacaagtga ttggctaaaa cacttggata atttacttct
     2161 cttctgggat aaaaaagagg cttatgccac aggtaaaagc gacttgtttc caacttatcg
     2221 ctgtaatacg ggtgaaactt tcgatataaa tgctaatccg ttacctgatg aaattgtgga
     2281 cgccttgaat agcgaagata ctaagggact tatagaactt gataagaatt ttatccgtgc
     2341 acactctcgt caaacctatg cctacggaat agcgttccat atgactggta aagggaagta
     2401 tctcaatctt tgtaagaaag gtgttgacgc cttgcttaaa gccattgacg gcaattatgg
     2461 tatgtgtgtg acacaaactc tttctggtaa gtgggataat gctcgtagtg atcgtacgag
     2521 ccaggatttg gcttatggac ttactggcct tgggatgtat tatttcttga ctcatgataa
     2581 aactattttg catcgaatta ttcaactcaa ggactacatt ttcaatgcct atttgagcga
     2641 tagacgaggg tatcttatgt ggttacctaa aaacaatcct aagagtgaag gggaagtgca
     2701 aattgtcgct cagctagatc aactttacgc ttatatgttg atgctcacac catgccttcc
     2761 tgaaccgtat aagagtgtct ggaaaaaaga catgaagaga attgcagata ttttgatcac
     2821 tcatttctat agtgaaaaat acaacatgtt ctggggaacc caaaataata tggatcgcat
     2881 gcagttagga acagatcata cggattttgg ccattccgta aaaacatttt gggtgatttt
     2941 aaaggtaggg cagcttcttg gagagccttt ctatgttcat tttgctcgtc ctaatattga
     3001 aaaaattatt aaagatgcct ttattccaag agttacgggt agtgctattc ctggttcttg
     3061 ggcgcgtcga ttcgatgcta acaatgagtt agatattgat aaggaatggt ggattcttgc
     3121 ggagttggat caagcggcag aaattttgag cattcatgat ccattcttct atgactacct
     3181 caatgaaact cagaagtact ggttagaagt aatgacggat aaaaaagacg gtgaaatttg
     3241 gcatatggtt gatggtaaga caaacgaacc cgttcatcga tttccaaaaa ttcataattg
     3301 gaagacttcg ttgcatagct ttgaacatgt tttgtttagt tatatgacgg cagcgaattt
     3361 tgcaaatgaa aacttccact tgtattatgc tttaccagaa tgggaaaccg cagaccaaga
     3421 taatgtggct ccgtacatgt tttttgctaa tgtggtaaag gttgaaaagc aaaaaagcga
     3481 tatacagctt gagttaccag acaataatga agtatataag gtgactttta ataaccttca
     3541 ttaaatcact tcaatataat caaatcaaaa ggtgacgcaa aatctcgcgg cacctttttt
     3601 atattacatc aaaagagagt caggctcttg gtccaaaatg acaaatacct gttgtcgttt
     3661 ttaaaaaagc gccctcgaga aaatctcaaa ggcgcctttt tttgtgtgtg tggtcttggt
     3721 cttattttct atcgtttttc cgcaggaatt ttctgcagaa actttccacc agctcgtagc
     3781 gttatcacgt aacgaccagc gggaagtgcg ttccactcgt tcgaaatcat acccccgaat
     3841 tgaacggagc gttcaaaaac agtattgcca tttacatcaa aaatgaagag ctttgcattc
     3901 gtctgcggaa cctgataagc gattccttcg cgagaaatgg ttaggaaagc actgccaatt
     3961 ttcgcgaggc gaattcccgt cagatcatct tctgaagtgg aatcgtcttt tgatgtatcc
     4021 gctggagcat tcggatcaat taccgtaatc acgcctgtct tcacataagc atcgagagca
     4081 ccgcctgttg tcgttacggt aaatccaaag actcccgctt ccgtcggcgt tcctgtaaag
     4141 ttcagttttc cgtttgccaa agacgttgta atgccagcag gaagtcccgt cgccacagca
     4201 ccagtgcagt gcgaatacgc atacgcaaag tccacaatcg gttcgcccaa gttgattgtt
     4261 tgcttcgaag agccagcgcc gtttttcacg agaagcggat ccagaatttc accgactagt
     4321 tcaatttttg gagtagggaa gttgcccgaa gcaagccaga atccgagatg cggtggctga
     4381 ttgtatgccg tattctgcca agatattccc aagcggtaaa ctggatcgtg catcagcgtg
     4441 tacatccgat gtgttgttgg aatcgttgtc gtgtagagga acagatgcga atcatcgtgt
     4501 aaaatcactt cttcacgcca gtcgcctaag atatcaccgc tcaaattcgg agttgccttg
     4561 gtggtgttgc aagaagcgcc ttccatcgta agcaatgtcg tatttttttt cgttgagctg
     4621 ttccacttac tgattgtcac attgtctaaa agttcgtcca gaaggtcacc atcccagtag
     4681 acgcggaaat tgtacgcggg tctggaagat gctagtattg ttcccttcac tgaatacgtg
     4741 ttgtaatttg ccccagacca catctcggat ccgcgggagg ttgaatccac gtctgctgct
     4801 acaccgcgtc cgttatcgcc gctgctcttt tcgtaaaaca ggcgctctcc cgtccgtgca
     4861 tcgtgcaagt cgtagccgta aggagaatct tcgtgtactt gccaaacttc taagccttcg
     4921 cgatctggat ccaagtcgcc caagtgcatc gcatcaccgt ggccaagccc cgtgcgatac
     4981 atgaacttgc cgttatgatc aatggcgcag gaaccttcga taatttcatc atagccgtca
     5041 ccgtccacat cgcccgctga aatgttgtga tttccctgac cgtagcattc ttggcctttt
     5101 gtcggtgaat tataatacca acgctgcgaa agcgttttac cgtcccagtc gtaagcggtc
     5161 acagccatgc gagtatagta accgcgctga aagaccatac ttggcttgtt gccgtccaaa
     5221 taagcgttcg tcgctaaaaa acggtcgacg cggttaccgt aactatcgcc ccagctgctc
     5281 accgttccgc gggcaggatt gtagttcacg gtcgccattt cttttccagt ctggccgtta
     5341 aaaatcgtta gaaattcttc gccactcaaa acgtagcctt tcgaattgcg ataatctttg
     5401 gaatggtcca cgcttgcggc aggtccgttc gaaaggtagc tacccgttcc gtcttttgtt
     5461 ccaggagccg ttttcatcgc cacttccgcg aaaccatcca agtcgtagtc ggctacgaga
     5521 atctgcgtgt aatgagcccc agcgcgaatg ttcttgccca aatccaaacg ccatagcttt
     5581 ttgccattta atttataggc atctatataa acgttgcctg tataaccact ctttgaatta
     5641 tcctgggaat tgctcggatc ccatttgaga actagttcgt attctccgtc gccatcgaga
     5701 tcgcccactg caatgtcgtt aggcgagtac gtgtagctac cgctcgaatt ggaaccgcca
     5761 gcgggtctgt ctaaattgat cgtaagcttt tggctgctcc aaacgcttac cgatttttct
     5821 gcgcctttct cttcgccgtt tataatgggg cgcacttcat ataaagagga tgttgaccct
     5881 tttgtatcgg tgtaattagt cgctccagta atcgggcttg cgttcactag ggtaccatcc
     5941 cgatacaaat tgaacgatgt cgtcgccgcg tcgttaccca aaagtcgcca gcttaaaaag
     6001 acaccaccat ctactttcac accgactagg ccacggtcta aaaattccat ttgccttact
     6061 gctcctgctg tggaagcaag agacagtgct aatatcgctg agacaaccaa tcccttcata
     6121 atacacactc ctatgttata acaaggaata tatacgtctc aatggcaatg ctaaaaaaaa
     6181 ataaaaatta ccattgtcca ctgacaacgg cgataatgga ttacatctgg ttactttgac
     6241 gtattttcaa acaattcttt cattttacgg ttatcgccta tagactgaac acaaggttcg
     6301 gtgaaaacga agagtatttg cttctttgaa cgatgccatt tgccatatgt tccatcgaga
     6361 ttcttttcta ctgtgctgat ataatctaga acaaaagaca ttgtatcaag acaaaaaaga
     6421 gttataaaag taaagccatt tttttgtaag gaatcacagc tcttcttatc taaatctata
     6481 gaatgtaaat ttttgatgtc aagttggtct tcgttaaagt gatgttgaat cacattcagc
     6541 tctccaatac tctctacata catatattcc caaatggagt cgtttatatc aaattcacat
     6601 gatgatttga attcattcac aagaaaatcg tcgtttttct acccagaatt acccactcag
     6661 ggtattaaat gtaactaaaa gtttacaaat aggcgccaaa gcgtatttta actgaacgta
     6721 agtcgttgaa aatcaataag atagctttat tttgctttag cgaaaaaatg atgagacaaa
     6781 cggcggaaat cgcttaatac aagtcttttt taggggactc tattgctgat agtggggaaa
     6841 agtggtatat tttgttgtgt tgtggcaaca agtgggttat aattcatgaa tcttgatttt
     6901 ttcattggcc aggctaaaac agccatagac gaaaagggaa ggacttcctt tcctagggaa
     6961 tttcgtcggc agttaagccc agaggaaagt gaaagtatgg tgctctctat agggcccgag
     7021 cggacactga ttctgtacga gctcaatgag tttaagaagt tcatgaccga attgaatgcg
     7081 cgtcctcgga cccgccaaac agaagcactt cggagtacta tccaaggcca cactagcttt
     7141 gtctcgttgg atgggcaaaa ccgtatttta ctttcaaaaa aatttttaga gtatgctaaa
     7201 atagaaagcg aagtattatt tgttcctcat gttggaaaat ctctgaaatt atggaatcct
     7261 gatatttatg aatcctcata tggctttagt agtccagagg attatgaaca atttgatgct
     7321 gggttcttta gcgatgtttg gatgggggac tcgagtgaaa aacaatagaa attatcacga
     7381 tcccgttttt ctaaatgaaa cgctaaaata tttatccatt tcttctgaag gtgtttttgc
     7441 cgactgtact ttgggcggcg gaggccattc cctcgctatc gtcgaacgtt tagggccaga
     7501 aggccattta cattgttttg accgagatcc agaagcgatt gcatttgcat cgaatagact
     7561 caaagcgttt gaaaaacaaa ttacgtttca tccagttcct ttttctgaaa ttgaaaatga
     7621 aatagatgct gattcattag acggtgttct gtatgattta ggaattagta gccatcaagt
     7681 agatgattct tctagaggat ttacttttgt tggtaatcaa ccgctcgatt tacgtatgaa
     7741 ccagcaggga gatgaaagtg ctcaacaatg gcttcgtcat atttcggctg aagatttagc
     7801 aaaagtattt tctcaaaacg ctgatcttga tcgttcttat aaattggctg tacgattgat
     7861 tgaaaaaatc caaaccatct ctggtgatat tttacctcaa gatgttcgag aagtggtaga
     7921 atcggttttt tatgataaac gtcgtgattt gaatagcatt ttagcaagag tgtatcaagc
     7981 cattcgcatg gaaataaatc aagaattaca acaaatagaa aaaagtttgc gtggtgctgt
     8041 gtcttgctta aaaaaaggcg gtcgacttgt tgttatttct tatcactctg tagaagatcg
     8101 ttgtgttaaa aatactgtaa gcgcttttga aaaagattgc atttgtcctg aaaagcttcc
     8161 cgtatgtagg tgtggaggca atcatcgttt gttgaaaaaa gaaattcgta aacctttatt
     8221 accgacttcc actgaaatag atcaaaactc tcgagctaga tctgcaaaat taagagtgta
     8281 tactaaagta tgacaggtgt cttaaaatca acggtaacag aacttactac aggtaaaatg
     8341 attttacttg tttctttgtt cttattgatg gttttagtgc tattcgtcgt accactttgg
     8401 cttcaaaatc gttacatcga attatcggaa aaagaaattc gtttttctaa tgaagtcatt
     8461 gaattaaaaa cagaaatcat tcgtctagaa ctttcgaata ataaacttac ctcttttgaa
     8521 aatctagccg actttgctcg cgatcatggt cttgattata atatagttcc cgttaaactc
     8581 atgtctgttg gagggaacta atgtatttag atcgtttggc ttggttggtt ttaatatttg
     8641 cttcgctttg gcttgtttta gtttctcgta cttttcaaat acaagtttta aatcaagaga
     8701 cctataaaga acatgctcag gaatatgcat tacgtcaaat gaatttatat gctgaaagag
     8761 gcaaaataat ggatcggcaa gggattgtct ttgctgataa tattaaagac aaagataata
     8821 agcatttaga gtatagccgt atttttctac aagggaaaat ggcttcgcag ttgattggaa
     8881 aagtgggtta caatggtcgt ggtgccatgg gattagaacg ccagtttgat gaaaagttaa
     8941 gaggaaatag cgggtatcta ttaagtgttc aaacagcggg tcgaaaaatt attcatggtt
     9001 tagaaaaaga ttatgaagag gcgattcctg gcaaaagtat ggttttgaca attgatcggg
     9061 atatgcaaga aattgtcgaa aattctttga aagatggtgt tgaaacattt aaagcaacga
     9121 gcgcaagtgc gattgtggta gatccttttt ctggagaaat tcttgcatta gcgagttatc
     9181 ctacttttga tcctaatcaa aaattgcaaa cttcagcgtc acgcgcgttt agaaatgatt
     9241 tagtctcttt atcttttgaa ccgggttcca cttttaaggt cattactgca gcggctgctc
     9301 ttgaagaaaa tgcagtgaat cctaaacgga aaatgagtgg ggaaaatggc cgttgggtat
     9361 tacctaatgg agaagtgatt cgagatacga aagatcatgg cgaaatggat atgactgaag
     9421 ccatgactta ttcttctaat atcgtttatg caaaaatagc tgattctatt ggctcagaaa
     9481 cattctaccg atttgttcgt gattttggtt ttgggacaaa aacatccgaa gatttaattg
     9541 gagaagaatc tggttactta aaagaacctc atacttggag cgggcgaaca ttaaaaacaa
     9601 tgggatttgg tcatgaattg actgtgactc ctattcaaat ggtcatggct ttttctgcaa
     9661 ttgccaatgg cggcaaatta atgcagccta tgattattaa agaatggaga gacgagaatg
     9721 gaaatacttt agaaaaaaat aaccctgtag aaataagaag agttgtttct gaaaaaacag
     9781 ccgctcatat acgtgaaatg ctttatcaag ttgtagtgaa tggaacggca aaaagcgtga
     9841 tgtcttcaaa aattccaaat gtagactttg gaggaaaaac aggaaccgct gaaaagtata
     9901 gccaagaatt aaagcgttac gatcgcaact ctcaagtagc ttcctttata ggcttttcac
     9961 ctgcaaggga tgtacgctat gtttgtttag ttcttatgga tgatccaaaa acgaaaacag
    10021 tgggtggttt aactgctggg cctgttttta gaaaaattat ggaaagtatt tatttccatc
    10081 caaagctttc tcctacgcct tataatttag tccaagtggc acaggataaa aagtgcgaga
    10141 ttgattttgt cgggatgtct gtggaaaatg caaaaaagct tgctgcaaat aagggatgct
    10201 ctgttcaagt tgaaggcaat ggttctgtta tcgtttctca aaaaattaat ggagaaaaag
    10261 acgcacaggt gtccttgaaa tctggtgatt taaattattc aaaaatgcct gatttaagag
    10321 ggctctcctt aaaagaggct ttagaagcga tgagtctcat tcgtatgcct gttgagtttt
    10381 cagggaaagg acgcgttcta gaacagttcc cagagccatc aacgatgata tcgaaagggc
    10441 aaacctgtaa attggtttta aaggaaaagg tgtaatatga tattcaaaga attactccaa
    10501 gactttaaaa taactggttt agctgatgat tctcgtcggg ttcaagaagg atatttattc
    10561 ttttgttttc cagtaaaaga aagtctttct tttgcaaaaa tggctttaga tcaaggtgct
    10621 cgagcaatcg ttgctgaaac agaagctcct ctcgaaatga agtcaaaatg gattcaagtt
    10681 aagaacgtga aagaagctcg tttagaagcg gctattcttt tttatggcaa tccttttgaa
    10741 caattaaaag tgcatgcgat tacaggtacg aatggtaaaa cgacaagtac ttttttaatg
    10801 gaagccatgc tcaatgcggc tggtagaaaa acggctttga taggcaccat ttgtaataag
    10861 attggtaatg aaatcattcc ttccttttta acaactccag gtttacttga tctttttgct
    10921 tttgcaaggc atgctcttga ggcaggctgt acggatttag tgatggaagc ctcttcccat
    10981 gcattagatc aagggcgtgt agctggtatt tcttttaaaa ctgctttatt tagcaatttg
    11041 actcaagatc atttagatta ccatttgaca atggatcttt attttgaggc gaagaaaacg
    11101 ctctttacaa aatatttgtc taaagaaggt catgctgtca ttaatgttga tgatgcatac
    11161 ggtaaaatgc tttttgattc tttagatggt aataaaatgg ctgtttctcg aatgaagtct
    11221 aaagaagcaa tgatttatcc agaagggaat gtggctcaaa gtgaaagagg catgactctt
    11281 cttgtgcctg ctatttctaa tcagcctttt gaaacacatc tttgtggtga ttttaatgtg
    11341 gataatgtgc ttttagtttt aggctttgca aaagcacttc agcttccaga gatggctatt
    11401 cgaaaagcgt tagagaatgt tcgagttcat ggtcgatttg aattggttta taatcagaat
    11461 aaaaaacatg tagttgtcga ttatgctcat acgcctgacg cattacaacg cgttttacaa
    11521 acggctcgtc gcctttgtca gggtaaactt gctgttgttt ttggttgtgg tggagataga
    11581 gataaaacga aacgtccatt gatgggatct attgctacaa aaaatgcaga cgtcgtgtgg
    11641 gtgacttctg ataatccaag aacagaaaaa ccagaagata ttattcaaga tattttgaaa
    11701 ggcatttcaa aaaaagaaga tctctatgtg attgaaaata gagcggaagc cattcaaaaa
    11761 gcttgtgtat cgatgaatga aaatgattgg ttagtgattg ctggaaaagg gcatgaaaac
    11821 taccaaataa tcggtcaaac gaaacatcat tttgatgatt ctgaagaagt tcaaaaggcg
    11881 atgctatgat tatgaaactg gatttaacca ttgctgaaat gtgtgaaatt ttagaaacca
    11941 ctccagttgg tttaaaaaca cgtgaacttc aaaaaaaagt acaagtttgt atggattctc
    12001 gtgaagcggc caaaggatgc gttttttggc ccattcgagg agttcgtttt gatgcacatg
    12061 attttattac ccaagtcgaa gggaaaggag cgcttatgag cgttgtcaat gaagattatg
    12121 ctggtttaaa atctattaaa gcttatgctc cagtggatga ttcatcagaa gcattgctta
    12181 agctagctaa agggtatcag cgtcgttttc acttgaaaaa agttgccgtt actggaagta
    12241 acggaaaaac aacaacgaaa gagatgttgc gtgcagttct ttctactcaa tttaatactc
    12301 atgcgactca gggaaattat aataaccata tcggcgtgcc gatgacttta ttccaattga
    12361 aacatagtca tgaagccgcc attattgaga tgggtacaag cggtcctaat gagattaaac
    12421 ctctttctct tgccgcagaa ccaaatattg ctgtgattac aaatattggt gcaagccatt
    12481 tagaaggctt gggttctcta gaaaatgttt ttaaagaaaa attaagcatc gtttctggac
    12541 ttaagaaaaa tggcttatta gtagtcaatg cagatgatgc tttcttatct aaagtacgct
    12601 caactaagag ttacaaagtc attacttttg gattacgtcg aggagtgata aaaccaactc
    12661 atttgaaatg gaatgaaaat gcttgcgctt cttttgatat agggcgaaca catttcgaat
    12721 tgaaggtggc tggaattcat aatctttata atgctttagc tgctattgcg gtgggtgttt
    12781 ctttgcgtat tccaaaaaca cagattgcaa aagccttatc atcttttaga gcttcaagca
    12841 tgcgtatgga aatacataaa gcgaacggct ttagagtggt tgctgactgt tataatgcaa
    12901 atccttcttc aacacgtatg gcgttagaaa cgattggtaa tatctctacg catgaagggc
    12961 gtcgtattgc tgttcttggc gatatgttag aattaggtgc cgatagttcg aaattacatc
    13021 atcagattgg acgattgatc cctgaaatga attttgattt tttaatcgct gttggaaaag
    13081 atgcaaaaga atttcaaaaa ggggctattg aaggaggcct ttctccagaa aaagctcttt
    13141 attttgaaac cactttagag gccatgaatt ttttatctga agaagtgcgt cagaacgata
    13201 ttcttttgat taaaggatct cgtggtatga aaatggaaaa aattgtggaa atgttacttc
    13261 gcttggagcc cgtttacgga gtataatgat ggatcgtttt ttaaacatga agtttgataa
    13321 agggttactg ctagcagtaa ttctgcttct tgttttaggt atagtcataa tttatactgg
    13381 tacttctcct attgctatgc aaaaaggatt ggcagcagaa tatttcacca tttctcatat
    13441 taaaaaagtg gtgcttggct tgttcatgat gtgtgttgca cttgtattag attattccgt
    13501 ttggtataag ctcgctcgtc ctttatttgt gatttgtctt ctcgcgatga ttgctgtttt
    13561 atttcaaggt gattcagtga agggtgcttc tcggtggtta agtatagcag gaatgaaaat
    13621 acagccttct gaatttatga aactaggaat cttttgtttg ctttctgtaa agcttgtaga
    13681 agcaggagat cagattaatg attttaaaac aggatttttt aggcctttaa ttttagtcgg
    13741 tattgtttgt gtcacattga ttcttcaacc taatttttca atggttttaa tggttttagc
    13801 tacatcactt attctaatct atgtggctgg tgtaccaatg aagcatttga tgacattaat
    13861 tattttccct cctattttat caatcattgc atacactcag tcttatcgat ctttgcgtat
    13921 caaagcttgg cttgatccag aaagctatcc tgagaaggct tatcaattac gccattcgtt
    13981 aattgctctt ggtaacggag ggtttacagg tactggtatt gggcaaggca ctcaaaaatt
    14041 aggttatgta ccagaatcct ataaagatgc tgcattttcg attcttggag aagagcttgg
    14101 ttttattggt gctttcttaa tgttgagttt gtttgcgttt cttgcttata gaggttttgc
    14161 tattgcgcgc tcttcaagta cgcgttttgg aaagtattta gctgtggctt taacatcttc
    14221 tatttgtttg aatatgattt ttcatgtttg tgtgtgtaca ggtttatttc caacaacagg
    14281 tcaaccgttg ccctttatca gttttggcgg gacaagttta gtaatgacac ttctttcgat
    14341 tggaattctt ctcaatatat caaagcccaa tacagggcag aacattagag aaattccaat
    14401 caattccgcc ttttattcta cgaggtattt atgaaacaga aaagattttt atttgtttgt
    14461 ggcggtacgg gtgggcatgt ttttccagct ctcgcgatcg ctgaaagctt aaagaaaaaa
    14521 ggcatccaag aaatttattt tgcaggccga gcgaactcca tggaacaacg gcttgtttct
    14581 cctcattggc ccttttttga aatgactgct gaaccattac gtcgcggaag ctttgttaaa
    14641 aacttagctc ttccttttaa gctaagtaag tcttatattc atgcaaagca tcttatcgaa
    14701 aaaattaatc ctgatgtgat tatcgcgact ggtggctatg tttctttccc tattgttctt
    14761 gctggaggac gtttaggaaa gcctgtatat attcaagaac aaaacgcgat tgcaggagtg
    14821 gctaataaat atggtgcacg ttatgcttcc atgatttttg tgacttcaga agatgctgta
    14881 aaagcatttc aacatccaaa tgtaaaagtg atgggaaatc ctgttcgtga attgcctgca
    14941 aaagaaactc tcgctcgtcc tgctgaatac tctaaaggga aaaaagcggt ctttattgta
    15001 ggaggctctc agggtgcggt tggaattaat aataaaatcg aagaatctat tggtcgtatc
    15061 acttctagag atgatgtctc tgtggtttgg caagcgggtg caaaaaatgt agatgcattg
    15121 catgaacgct taggcatttt accaaatgta accatctgtt ctttcttaga taatatttat
    15181 gcctatatgg ctcatgctga tttaattatt agtcgtgcag gagcatccac attagcagaa
    15241 attttagctt ttggaaaacc atcgatttta cttccttatc cacatgctac agcaaatcat
    15301 caagaacata atgcccgtgc tgttgagcat agtggtgctt gtttggtgga actggataat
    15361 gatcctaatg atctttggaa taaagtagaa gaattactcg ctagtccatc aaaattagag
    15421 caaatgtcaa aggctgcaga aaaattaggc tctccagatg ctgcagataa aattactgaa
    15481 atcattttaa aagcggagcg ttgtatatga atttcatcag tcgtaaacgc atacaacggc
    15541 ttcattttgt aggcattggt ggtgctggca tgtctggtat cgctgaagta ttgcatgaaa
    15601 atgattttat cgttactggt tctgatacca gcgatggaga ggctattcaa taccttaaaa
    15661 agttagggat tcgtattgat tccattcacg aagcaaaaaa tgttgaagaa gctgatttag
    15721 tcgtttattc ttctgccgtg cagcaagata atccagaatt agtagaagct agagctcgtc
    15781 gtattcccat aatacgtcgt gcggaaatgc ttggcgaatt aatgcgatta aaatatacgt
    15841 tagcgattgc aggaactcat ggaaaaacaa cgactacttc aatggttggc gctatttggg
    15901 aaaaagcaga attagaccct acagtcattg ttggtggtgt tgttaaagga aaaggaagtg
    15961 gtgcaaaggt aggacgaggc tcttatttaa ttgcggaaag cgatgagttt gatcgtagct
    16021 tcctttcaat gatgccaagt tctgcaatta ttaccaatat tgatgaagat cacttagata
    16081 cttataaaaa tttagaagaa atcaaaaatg cttttgtgga atttgcaaac aaggttccat
    16141 tttacgggca agtgattata tgtcttgatg atctaaatgt tcaatctatt ttatctcgtt
    16201 tgacaaaacc tatcattact tatgggttta gccgtcaagc gatatatcgt attgaaggat
    16261 taaggagaga acaagagaat tttgtttttg atgtttttaa gaataatgag ttattaggaa
    16321 ctttcaaaat aaagactcct ggtaaacata atgttttgaa tgcaaccgct gccatcgcgc
    16381 ttgctgtaga agaacaaatt cctttgaatg tgattagtga agccatcgct tcttttgaag
    16441 gtgttcatcg tcgttttgaa tatatgggtg aatataaggg agctcttgtc tataatgatt
    16501 atgctcatca tccaacagaa gtggctgcga ctcttcaagg atttagagac gcatttcctg
    16561 atcgtcgttt gattgtcgtt tttcaaccac atcttttttc gagaacaaga gatcaccacg
    16621 gtttttttgg aaccgcattt gggaattgtg atattttgtt tggctgcgat atttatccag
    16681 cgagagaaaa accaatagaa ggtgttactg gagaattagt ggtgcaaagt gccttggata
    16741 gaggccatcg agatgctcgt tttattgggg caaaagaaaa tgctttgatt gcattgaaaa
    16801 aagaactcaa cgaaaaagat atcgttgttt ttatgggtgc aggtaatatt tggttgcaag
    16861 gaaaagagtt attggaggat aaaatttgac tcgagacaga accatattta gcagacgtcg
    16921 tttgggatat aacgaagtct tgcgaaaaga acgtcgccaa gaaaagctta aaaaaagcgt
    16981 tggcggtagt tttaaatggt ttggtcaaaa aggttggatc gctgctttag ttttagttgt
    17041 tttaggtgtt gcattatggc agggacgtta ttatctaaaa gattttaatc caacagaatg
    17101 gagatcgcta aaaacaattc agattaatgg caatcgtatg ttaacatggg aagaaatcat
    17161 acaaatttct cgaatagaag tag
//
LOCUS       DAOOBB010000020        16774 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_4489_length_16774_cov_4.663317, whole genome shotgun sequence.
ACCESSION   DAOOBB010000020 DAOOBB010000000
VERSION     DAOOBB010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 16774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16774
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4489_length_16774_cov_4.663317"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="PLT31_01885"
     CDS             complement(<1..207)
                     /locus_tag="PLT31_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93915.1"
                     /translation="MKYKLVLGCLGVFFIMSVIVACGEGAIDPFSTDDTVAQLSTASL
                     DVGKSINACLADPVCREKYESTETP"
     gene            complement(316..1566)
                     /locus_tag="PLT31_01890"
     CDS             complement(316..1566)
                     /locus_tag="PLT31_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93916.1"
                     /translation="MNKKLFFGIISIAFIAGIFVACGEGAIEAYNTDDNVADLTFGNS
                     LDIKKSETACQANPECLEKLNQVQPTSSAEPPPPSSSEEPVLISSSSVMFPTSSSSLI
                     TITSSTTIILPPSSSTIVIVSSSSVITPEGGLAGTCAPDPAIAELDTSVTWSFKKDPS
                     VTPQQVIAASYDWIFEGASVETFSGQGTLGMKKTVSYNTSGPHTTSLSINGTAPIACT
                     PVQVNGAKITGCKCLVDNASPDVAAGGVANWTVAGCASTANITGYTWNGGALGDVLTT
                     SHTFAAKDETFTPTLTVSNDDNTVQVVACPAATAIDATKPDYEMTASGDSVTFEGNVD
                     ATIVMNLPETWHNGTTGTCTFACQVNRGGGSGAISGTVDKVDITGSDYVTAPIPITST
                     INGYALKLVVDVGTNAGVTCFVGW"
     gene            complement(1784..2569)
                     /locus_tag="PLT31_01895"
     CDS             complement(1784..2569)
                     /locus_tag="PLT31_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW93917.1"
                     /translation="MPNVNINPDDVMIKLRGLKKIFGSQDVLLDVNLDIRRGETMVII
                     GQSGSGKSVILKHMIGLLQPDEGEVAVDGITISTPKYFDARTIRRKMGMLFQMGALFD
                     SMDTGENIAFALREHHPELTEAQVQKVVTENLKLINLVPEFRVKMPSELSGGMRKRVA
                     LARAIALSPEILLYDEPTTGLDPITSDVINDLILDMQSKLGVTSVVVTHDMVSAFKVA
                     DRIAMLYKGRIIEVGTVEEIKDTENPFVRQFITGQRKITSEEE"
     gene            complement(2581..3363)
                     /locus_tag="PLT31_01900"
     CDS             complement(2581..3363)
                     /locus_tag="PLT31_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW93918.1"
                     /translation="MSWWRYPIEGIGKILISGISNIGEIVCILIRTIKCIPYVHKNVG
                     LIVKQMVSIGVSSIPLLFVTSIFTGMVATVQAEFQFRDLVADKFVGTAACKMILIELG
                     PILTALVLAGRVGSALAAEIGSMREKEELDAYTVLGLDTYRYLALPRFVAFITMVPCL
                     TVISNCLALIGGWIVCVLALDITSYTYYTGMQYLFQPMDLWAGVIKSFVFGVIIFVLG
                     YYHGIKAGAGARGVGVATMNVVVSSCLMILIADFALAAVLFK"
     gene            complement(3360..4733)
                     /gene="dnaB"
                     /locus_tag="PLT31_01905"
     CDS             complement(3360..4733)
                     /gene="dnaB"
                     /locus_tag="PLT31_01905"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HPW93919.1"
                     /translation="MADFLDSSSSAQRQVPQNIDAEVNLLGGLIQDAEGFTEILAILD
                     EESFYLEKHQIIWKALTHLYRQGVVIDLVTLATTLETQGKLQFIGGKEYLFNLIESVA
                     SSANVLWHAEIIRNKYILRKLISNSNETIRAAQDPAASPDEILQESERQIFNLAEKQV
                     RDSMRPIEAHITTLLTKLKDRKDGLTGCPTGFTELDSLTNGLQPSDLIILAGRPGMGK
                     TAFALSLASNAAINYGQKIAFFSLEMDGEQLVQRILCSRAQVNMSNLRSGRLSRDDYQ
                     KINAQVQPILESKFFVDDNSDLGIMELMSKTRSLKRKSGLDLVVVDYLQLMKTGKEEN
                     RAVAIGAISRGLKILAKDLRVPIIALAQLSRKAEEKGRERPQLSDLRESGSIEQDADM
                     VWFVERPAYRTHAEEDKYKAQLLVAKHRNGSVADIDLTFMPEYTTFYNPSPDAFMDQD
                     NDFGEYV"
     gene            complement(4749..5552)
                     /locus_tag="PLT31_01910"
     CDS             complement(4749..5552)
                     /locus_tag="PLT31_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93920.1"
                     /translation="MPIEQSALKSYLQGQMELGEAEVYFDEPWTLAPKNLSKVPIKLQ
                     SKNVVEASLRAPVSSPSSVVNSAASLSTSLISDTEPVSELYAFQKARHLNDFNAALAE
                     DPIYAKGAPVILGTGALRPKAMLLFASPSKMDLAAGGIWKTEAGLMLERLFQSLNMEQ
                     NQLYYTYFYKREALRPISSLIEIQLRKMISKEIALVQPELIVLFGESSLQHIFGRGKT
                     FAQSVGTAMEFEGINTTVLVDPYEMMEDKNLKRLTWNVHIPASGFFVKR"
     gene            complement(5543..6763)
                     /gene="coaBC"
                     /locus_tag="PLT31_01915"
     CDS             complement(5543..6763)
                     /gene="coaBC"
                     /locus_tag="PLT31_01915"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HPW93921.1"
                     /translation="MELKGKKILLGVTGGIAAYKSVELLRLLQKQGAEVRVVMTPAAT
                     RFVTPLTFASLSKCPVYTQDGAPEARPYQHIDFPRWADLYLVVPCTANTLGKMANGLA
                     DDPVSLCFMSCAGEKWIAPAMNTVMYLSPAVQANLKRIREFPQVHVLESPSGMLACGE
                     EGPGRCAEPASIVEALLLAPEKNNPAWISGKKVLITAGRTEEAIDPVRYISNRSSGKT
                     ALALAFAFKNAGFSVTVVSGPMEASRPSEIEWISVKSALEMHHEVLSRASQFDVLVHA
                     AAVADYRPKTTSDEKIKDSRSHLVLELEPNPNILRDSVAEKKEGQVIVGFALETADAE
                     RHAKEKWQKSGADLLVLNTPVAKDSGFGFDQVSYSLISKDQPVTSPQMGSKDSLAQRV
                     VSFVVTSLGGHSCQ"
     gene            complement(6804..7952)
                     /locus_tag="PLT31_01920"
     CDS             complement(6804..7952)
                     /locus_tag="PLT31_01920"
                     /inference="COORDINATES: protein motif:HMM:NF033936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SO_0444 family Cu/Zn efflux transporter"
                     /protein_id="HPW93922.1"
                     /translation="MTLFIESFISLFVEMAPYLLLGFLLAGVLHVWVPKSFYLPKISK
                     PNFKSVLWATLFGIPLPICSCGVIPTAIALRREGASRGASVSFLISTPSTGVDSILAT
                     YSIMGFPFAILRVIAAFFTAIIGGLFTNWTTKSEAKPVESISLSEEKCGCHKEQENEK
                     TCCEKKSEKSCCENEDEDEVHFNEKSPKTLQERFIEMWRYGFVELLGDITRWLLVGLV
                     FGALISAFVPNDFFLGLRQYPILASLLVLVLAMPMYTCAIGSIPLAMALVAKGMTPGA
                     ALVLLMAGPATSMASIFVIGKSMGKRTLVGYLLSIAFGALFVGWVIDTFFMEFFLSSI
                     LPQSGLLTENHHISVLDSFASVILAILMIYSFRSKKTKKEGKKSCSCH"
     gene            complement(7949..8284)
                     /locus_tag="PLT31_01925"
     CDS             complement(7949..8284)
                     /locus_tag="PLT31_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPW93923.1"
                     /translation="MKSSKGKTNKEEISLDVLFELSEFFKFFSDTTRIRIIEILLHGE
                     MSVNDIADKLNIGQSAVSHQLRLLRTSHLVRPRRDGRTVFYSLDDEHIGLIFQTGLNH
                     ILHKKGVLP"
     gene            complement(8423..10210)
                     /gene="rpsA"
                     /locus_tag="PLT31_01930"
     CDS             complement(8423..10210)
                     /gene="rpsA"
                     /locus_tag="PLT31_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HPW93924.1"
                     /translation="MSKILYGTQDDLKEILEAEAELGSIGHKEAADVYAGMDCLEPGK
                     LVCGKISQVNEQEVLVDVNFKSEGVIARDEFKDTDSLEIGTEIEVFVEKLEGDDGRLV
                     LSKQKADFVRVWDRIHEAFENNEVVRGTLTKRIKGGVVVDLFGIDAFLPGSQIDLRQI
                     PDINALIGQDYDLKVIKVNKARRNIVVSRRVVLEEERNRQRGDVLETLEKNQVRKGIV
                     KNITDFGAFIDLGGVDGLLHITDMSYKRINHPTEMVQLGNEVEVMVLDFNDKKERISL
                     GMKQLKPHPWKDVAARYPEGMTVHGKVVSITDYGAFIELDSGIEGLIHVSEMSWTQHV
                     KHPSKILVVGQEVEAVVLKVEEDAERISLGMKQLETDPWDSIESELPPGARVVGEIRN
                     LASFGAFVEIKEGVDGLIHVSDMSWTKKITHPNELVKKGDKVECVVLAVDCEKRRISL
                     SMKHLTEDPWDSVESTYPVDAEVKGTIVRMLDRGVVVEMADGIEGFVPVSKLTAEYIK
                     VPADAFKVGDEIPAIVTEIDQNNRKLYLSVVDYFKNRESAELEAWMNSHKPGENNTSI
                     GEAIAPAKKKTTKKKAEKAEKTEEAPAAE"
     gene            complement(10247..10918)
                     /gene="cmk"
                     /locus_tag="PLT31_01935"
     CDS             complement(10247..10918)
                     /gene="cmk"
                     /locus_tag="PLT31_01935"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HPW93925.1"
                     /translation="MSESKNHIVIAIDGPSGTGKSTTAKLLSKNLGITYLDTGAMYRA
                     ITYVAMTSGVTPSDKEALLSLLQKTVIDFDSNNQVLINGFSQESAIRSPEVSEQVSYY
                     SAVPEIRSALTAKQREIGNKHSCILDGRDIGTVVFPNATFKFFLVTDLKVRAARRYAE
                     LIGQGKQVTLQEVEENLRLRDSIDSSREVAPLLKAPDAIEIDTTQLSIQEQVEKIQAI
                     VSVVA"
     gene            complement(11011..11643)
                     /locus_tag="PLT31_01940"
     CDS             complement(11011..11643)
                     /locus_tag="PLT31_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93926.1"
                     /translation="MKKGLMVLFAFLMVLSGCAGSKSSNPDQINDALVGFTTSVQGVR
                     WEEALKYVTEDEVYAISDGKEFKPEYQVAARRLKLSTLKQMELYVDGSGRLVGIKKAM
                     DDSNERYKMSEDQAKVGLDLEKREQERIKRRLEEGQKILEEESKPQEPEVQVFTNKLT
                     EEEKRKYGSTKELLPPEEYSDENTQEAEEVLETEDSDNMESSDFYEDTEY"
     gene            11759..12931
                     /locus_tag="PLT31_01945"
     CDS             11759..12931
                     /locus_tag="PLT31_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HPW93927.1"
                     /translation="MNTSSDIYRLTLPENREIILVGTAHISQESKDLVRKTIEEESPD
                     TVCVELDEGRMKSLEEPDRWKNLDLKAIIRQKQLGTLVANLVLGSYQKRMGDQTGVRP
                     GAELQEAVQIAKEKNIPIVLADRQIKTTLRRTWSYTPWYRKFALLGALISSIFDKTEV
                     SEEELRKIKEQDSLNAMMAEFGKTFPEVKKVLIDERDQFLASRIINAPGKKVVAVVGA
                     GHVKGISQIIEQNMELPSEDSLSEIKKASLTFKILAWSIPIAIVAAIVAVGFQSGIAR
                     AGELSLHWAMLTGGGAMLGTIIAGGHPLTVLIALLTAPITTLIPMIGVGFFTALTQVY
                     MRPPRVQEMETLNDDIWKVRRWWKNRVTRVFLCFLLPGLPTVIGKVLAILKIYQAF"
     gene            13120..14256
                     /gene="rffA"
                     /locus_tag="PLT31_01950"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             13120..14256
                     /gene="rffA"
                     /locus_tag="PLT31_01950"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HPW93928.1"
                     /translation="MKIPFNKPPFVGTELDYIKQAVHNGRICGDGEFNLKCHEWLENY
                     SGCAKALLTTSCTHALEMAAMLCDIKPGDEIIMPSFTFVSTASAFVLRGAKIVFVDIR
                     PDTMNLDETKLEDAITSKTKAIVPVHYAGVACEMDTILAIAEKHGLFVIEDAAQGMMA
                     SYKGKVLGTLSHFGCYSFHETKNYSMGEGGALLINKKDFVKRAEIIREKGTNRCEFHR
                     GEVDKYTWVDLGSSYLPSELNAAYLYAQLLEAQKILSNRLNCFQFYNEHLEILECRGL
                     IQRPHIPAECEHNAHMFYIKVDSIETRTALLSHLTKNGILAVFHYVPLHVSPVGKIHG
                     RFHGEDQYTTSESNRLLRLPLYYGLQEKDQEFVVQKLNEFFLRK"
     gene            14312..15514
                     /locus_tag="PLT31_01955"
     CDS             14312..15514
                     /locus_tag="PLT31_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPW93929.1"
                     /translation="MHTVNSKRLLLLGGSHAEIPLIKAAQKLGYYVITTGNARDGLGH
                     PVADKNIFADFSDKEQMLSIARAEGVSAVCSGCNDFALLSTVFVTEKLGLKGHDSLKI
                     SETIHHKDRFRALAEKLEIASPKAVQVKSIEDLEKALLTLKFPLIIKPVDLTGGKGVR
                     RVETAEEAIDAYHHAFERTRENHIIVEEFIEGTNHGFSAFLVDQKVAFYFSDNEQYYL
                     NKYMVSGANSPTIVCESSLKRLCTYSEKIAQDLQLVDGILHIQFIEHPTLGPYIIEIC
                     RRPPGDLYIQFVEYATGVPYPEYLILAETGADVHSIKQAPTKGFWLRHCIMAKEEGVL
                     EDVYFAPEIKNNVFDKLLWYKKGEKVEDKLYYKAGIVFLKFDSLKEMQEKTTLMNEYI
                     QCKINPIK"
     gene            15490..16632
                     /locus_tag="PLT31_01960"
     CDS             15490..16632
                     /locus_tag="PLT31_01960"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPW93930.1"
                     /translation="MQNKPYQIAFIGGGINSAVGETHKIASQMDGCFQLVAGSFSSHA
                     KTNEETAIAWNVPKERLYENYLTLLEKEKGCLDAVVILAPTDHHKEIVIQALRHGYPV
                     ICEKSLATSYEDGLEIKKVVEETHGFFCITYNYTGYPMIRELKRLIKDGRLGTIQQIQ
                     MEMPQEGFLRLGPNGEPPKPQAWRLHDTVIPKISLDLGTHLHNMILFLTEKNPQRIIS
                     DQTTFGLFPQIVDNVFALAQYPENMKAQIWFSKTALGQRNGLRIRVFGNKASAEWFQM
                     DPETLKFADVYGNISLMDRTTQVQEANKARYNRFKAGHPAGFIEAFANYYRDVADCLT
                     EYKKTGQFESPFVFGIKPSCDGLSMMQAAARSATNGQWEFVFYESL"
BASE COUNT         5208 a         3473 c         3215 g         4878 t
ORIGIN      
        1 aggtgtctct gtagattcat atttttcacg acaaactggg tcagcaagac aagcgttaat
       61 agactttcca acgtctaagg aagcagtact tagctgagcc acagtatcat ccgtactaaa
      121 tggatcaata gcaccttcac cacaagcaac aatgacactc ataatgaaaa aaacacccaa
      181 acatcccaat actagtttgt atttcatatt ttacctcatt ggcgctacaa gatagaaaaa
      241 aaaatattct ttttatagcc ctaaaaaaca agcacctctc aaaagaggtg ctttatagga
      301 aatgaaaaaa taattttacc aacctacaaa acaagtaacg ccggcatttg ttccaacatc
      361 aacaactaat ttcaatgcat aaccgtttat cgtacttgtt ataggaatag gtgccgtaac
      421 ataatcagac cctgtaatgt caactttatc tacagttcca gatatggcac cgctaccacc
      481 acctctattc acttgacaag caaaagtaca agtccccgtc gttccattat gccatgtttc
      541 agggagattc ataacaatag tagcatccac attcccttca aaagtgacag aatctccaga
      601 agcagtcatt tcataatcag gctttgtcgc atcaatagca gttgctgcag ggcaagctac
      661 gacttggacg gtgttgtcgt cgttagaaac ggtgagtgtt ggggtaaagg tctcatcttt
      721 cgcggcaaag gtatgagaag tagttagcac atcgcctaaa gcacctccat tccaagtgta
      781 acccgtgata ttagcggtgc tcgcacagcc agccactgtc caattggcaa caccaccagc
      841 cgccacatct ggagatgcat tatctacaag acatttacat ccagtaatct tagccccatt
      901 gacttgaaca ggagtgcaag caattggagc cgttccattt attgataacg atgtggtatg
      961 aggaccagaa gtattataag aaaccgtctt tttcatacca agtgttcctt gaccactaaa
     1021 ggtttcaaca gaagcacctt caaaaatcca atcataacta gctgcaatga cctgttgtgg
     1081 agtcactgag ggatctttct tgaaactcca agtgacagac gtgtccaact ccgcaatcgc
     1141 aggatcagga gcacaagtac cagcaagccc cccttctgga gtaataacag aagaagagga
     1201 tacaattaca atagtactac ttgaaggagg taaaataatg gtagtagaag aagtaatggt
     1261 aatcaaagaa gaactagaag ttggaaacat taccgaagaa gaagaaatca atacaggttc
     1321 ctcagaagag gaaggagggg gaggttcagc agatgatgtt ggttgtactt gattcaactt
     1381 ctctaggcac tctggattag cttgacaagc tgtttcagat ttttttatat ctaaagaatt
     1441 tccaaaagta agatccgcga cattatcatc agtattataa gcttctatag ctccttcacc
     1501 acaagcaaca aaaatacctg caataaacgc aatactaatt attccaaaaa atagtttttt
     1561 attcatacta cacctctttg cccataatca aacagattac tctacaaaac actgttgatc
     1621 tgcattaata tatactaaag taaagagtaa agaaatattt tcaaataatc ttcttttaaa
     1681 aaacgcctta ttagtgcaaa aatgcactta tttttgtttc tttttcttgt tttaaaggga
     1741 aaagagccgt ttttttgaat tttaaaggcg atttgagcaa aaatcactct tcttctgagg
     1801 tgatttttcg ctgacctgta ataaactgac gaacaaaagg attttctgta tctttgattt
     1861 cctctaccgt acccacctca ataatacgac ctttatatag catagcaatg cgatcggcga
     1921 ccttgaaagc actcaccata tcatgagtca ctaccacaga agtgacgcct aatttactct
     1981 gcatatctaa aataagatcg ttaatcacgt cactagtgat aggatctaag cccgttgtgg
     2041 gttcatcata aagtaatatt tctgggctta gtgcaatagc ccttgccaaa gccactcgtt
     2101 ttctcattcc tccagagagt tctgaaggca ttttgactcg aaactctgga accaaattaa
     2161 tgagctttag gttttcagta acgacttttt gtacttgagc ttccgttaat tcagggtggt
     2221 gttctcggag agcaaatgca atattttctc ccgtatccat ggagtcaaaa agggccccca
     2281 tctgaaaaag cattcccatt ttacggcgaa tagttcttgc atcaaaatat tttggtgtac
     2341 taatcgtaat accatctact gccacttcac cttcatcagg ctgtaaaaga ccaatcatgt
     2401 gttttaaaat aacagattta ccactacccg actggccaat aatcaccatg gtttctccac
     2461 gacgaatatc aagattcaca tctagtaaaa cgtcttgaga accgaaaatc tttttaagac
     2521 ctcgcagttt gatcattacg tcatcaggat taatatttac gtttggcata cctacacctg
     2581 ctacttaaaa agaactgctg ctagagcaaa atcagcaatt aatatcataa gacaacttga
     2641 aaccactaca ttcatggtgg caactccaac accgcgagca ccagcacccg ccttaatacc
     2701 atgataatag cctaaaacaa aaataatcac accaaagaca aaagacttaa tgactccagc
     2761 ccataaatcc atcggttgaa acaagtactg cattcccgta taataagtat aagacgtaat
     2821 gtctaaggca agaacacaga caatccagcc tccaattaag gctaagcaat tcgaaataac
     2881 agttaaacaa ggaaccatag taataaaagc aacaaatcga ggaagagcta aataacgata
     2941 agtatcaaga cctaaaacag tataggcatc aagttcttct ttttctcgca tggagccaat
     3001 ttctgcagca agtgcacttc ccacacgccc cgcaagaaca agagcagtta aaataggccc
     3061 taattcgatc aaaatcattt tacatgctgc tgttcccaca aatttatccg cgacaagatc
     3121 tctaaactga aattctgctt gaacggtggc gaccatccct gtaaaaatag aagtcacaaa
     3181 aagtaaaggt atagaagaaa caccgattga aaccatctgc ttaacaatta agcccacatt
     3241 tttatgaaca tagggaatgc attttatggt tcgaataaga atgcatacta tttctccaat
     3301 attactaatc ccagagatca gtatttttcc tatgccttct attggatagc gccaccaact
     3361 catacatatt ctccaaagtc attgtcttgg tccataaaag catctggcga tggattatag
     3421 aaagtggtat attcaggcat gaaagtaaga tcaatatctg caacagaacc attacgatgc
     3481 tttgcaacga gtaattgagc tttgtattta tcttcttcgg catgagttcg ataagcagga
     3541 cgctctacaa accagaccat gtcagcatct tgttctatag atcctgattc acgcaagtca
     3601 ctgagttgag gacgctcgcg tcccttttct tccgccttac gactaagctg agcaagagca
     3661 ataataggga ctcgtaaatc tttggctagt attttcaaac ctctagaaat agcgccaatg
     3721 gccacagcac ggttttcttc tttgcctgtt ttcatgagct gtaaataatc tacaaccaca
     3781 agatctaaac cacttttccg ctttaaggaa cgagttttag acattaactc cataatccct
     3841 aaatcagagt tatcgtctac aaaaaacttt gactctagta taggttgaac ctgggcattg
     3901 attttttgat aatcatctct actcaagcga ccgctgcgaa ggttactcat attgacttga
     3961 gctcttgaac aaaggatacg ttgaactaat tgttcgccat ccatttctaa actaaaaaag
     4021 gcaatttttt gcccgtagtt aatagccgca ttacttgcta gagaaagggc aaaagccgtt
     4081 ttacccatac ctgggcgacc agctaaaata atcaaatcgg aaggttgcaa accattggtt
     4141 aaactatcga gttcagtaaa ccctgttggg caacccgtta agccatcttt tcgatctttt
     4201 aatttggtga gaagagtagt aatatgtgct tcaataggac gcatggaatc tcgcacttgc
     4261 ttttcggcga gattaaaaat ttgacgttca ctttcttgaa gaatttcatc tggactagcc
     4321 gctggatctt gtgcagcacg aatggtctca ttagaattag aaatcaattt tcgcaagata
     4381 tatttattgc gaataatttc ggcatgccaa agcacattcg ctgaagatgc caccgattca
     4441 attaaattaa aaagatactc tttacctcca ataaattgga gtttcccttg cgtttctaaa
     4501 gtcgtcgcga gagtcacaag atcaatcacc accccttgac gataaagatg agttaacgct
     4561 ttccatataa tttgatgttt ttctaaataa aaagattctt catctaaaat ggcaagaatt
     4621 tctgtaaaac cttcggcatc ctgaattaag ccacccagta gattaacttc ggcatctata
     4681 ttttgcggaa cttgtcgctg agcggatgat gatgaatcta gaaaatcggc cataatcttt
     4741 taaatatact aacgtttgac aaaaaaaccg ctcgctggaa tatgaacgtt ccaagtgaga
     4801 cgttttaggt ttttatcttc catcatttca taagggtcga ctaaaacagt ggtattaata
     4861 ccctcaaatt ccatcgctgt tccgacactt tgagcaaacg ttttaccacg accaaaaata
     4921 tgttgtaaag aagattctcc aaaaagtact attaattcgg gctgcaccaa agcaatttct
     4981 ttagaaatca tctttcgaag ctgaatttct attaaagagg aaataggtct taatgcttct
     5041 cgtttataaa aatacgtata ataaagctga ttttgctcca tatttagact ttggaaaaga
     5101 cgctctagca ttaagcccgc ttctgtcttc caaatacccc cagcagctaa atccatttta
     5161 gatggggagg caaataaaag catggcctta gggcgaagtg cccctgtgcc taaaatcaca
     5221 ggcgcccctt ttgcataaat agggtcttct gcaagtgctg cattaaaatc atttaaatga
     5281 cgtgcttttt gaaaagcata taattcagaa acaggctctg tatctgaaat caaagaagtg
     5341 gataatgatg ctgctgaatt gaccacagaa gaaggcgaag aaacaggggc ccttaatgaa
     5401 gcttctacaa cattttttga ttgaagcttg attggtacct ttgaaagatt ttttggagca
     5461 agagtccatg gctcatcaaa atatacttcg gcttctccta gctccatttg cccttgcaag
     5521 taagatttta aagcactttg ctctattggc atgaatggcc tcctaatgag gtcacgacaa
     5581 aagaaaccac cctttgagcg agagaatctt tggaacccat ttgaggagaa gtaacgggct
     5641 gatcctttga tatcaaagaa taagaaactt gatcaaaacc aaagccacta tctttagcga
     5701 ctggcgtatt taatactaaa agatcggcac cactcttttg ccatttttct ttggcatggc
     5761 gttcagcatc tgcagtttca agcgcaaaac ccacaatcac ttgaccttct tttttttcag
     5821 ccacagaatc tcgaagaata tttggattag gttcaagttc taaaacgaga tggctacgac
     5881 tatctttaat tttttcatct gaagtggttt ttggacgata gtctgcaaca gctgccgcgt
     5941 gcactaagac atcaaactga gaagcacgag ataaaacttc atggtgcatt tctaatgcac
     6001 tttttacaga aatccattcg atttctgaag gacgagaagc ttccattgga ccagaaacta
     6061 cggtcacaga aaagcctgca tttttaaatg cgaaagcgag agctaaagcg gttttaccgc
     6121 ttgacctatt agagatatag cgaactgggt caatcgcttc ttctgttcga ccagcagtaa
     6181 ttaaaacctt tttaccagaa atccatgctg ggttattttt ttcgggagca agaagcagag
     6241 cctctacaat ggaagcaggt tctgcacaac ggccagggcc ctcttctcca caagcgagca
     6301 ttcccgaagg cgattctaaa acatgaactt gaggaaactc acgaattcgt tttaagttcg
     6361 cttgcactgc aggagataaa tacatcaccg tattcatcgc aggagctatc cacttttccc
     6421 ctgcacaaga cataaaacag agagaaacag gatcgtcagc gagaccattt gccattttac
     6481 caagagtatt tgctgtgcaa ggaacaacta aataaaggtc tgcccaacga ggaaaatcaa
     6541 tatgctggta aggcctagcc tctggagctc catcttgagt ataaacgggg catttggata
     6601 gagatgcaaa tgttaatggt gtgacaaaac gagtggcagc aggagtcatg accacacgaa
     6661 cttctgcacc ttgtttttga agtaaacgca ataattctac acttttataa gcagcaatac
     6721 cgcccgtcac gccaagaaga attttctttc ctttcaactc catagacgtg aatatagcat
     6781 taattcttta ctcttctttt tattcaatga caactacagg atttttttcc ttcttttttt
     6841 gtttttttag acctaaaaga ataaatcatg agtatcgcta gaattaccga agcgaagcta
     6901 tccaatacgc taatgtgatg attttcggtc aaaagccctg attgaggtaa aatagaactt
     6961 aaaaagaact ccataaagaa cgtatcgatg acccagccaa caaataaggc gccaaaagcg
     7021 atactcaata gataccccac aagagtacgt ttacccatgg attttccaat gacaaaaatg
     7081 gaagccatac tcgtcgcagg gcctgccatc aaaagaacaa gagccgcgcc tggagtcatt
     7141 cctttggcga cgagagccat cgctaaagga atagaaccta tcgcacaagt gtacatgggc
     7201 atcgccagaa caagaactaa taaactcgct aaaataggat attgccgaag acctaaaaag
     7261 aaatcatttg gaacaaaagc agagatcaaa gcaccaaaaa caagccccac aagaagccaa
     7321 cgagtgatat cgcctaatag ctccacaaaa ccatagcgcc acatctctat aaaacgttct
     7381 tgaagcgtct ttggagattt ttcattaaaa tgcacttcat cctcatcttc gttttcacag
     7441 caagattttt cacttttctt ttcacaacac gttttttcgt tttcttgttc tttgtgacag
     7501 ccacatttct cttcacttaa agaaatactt tctacaggct tcgcttctga ttttgtagtc
     7561 cagttagtga aaagacctcc aatgattgca gtgaaaaaag cggctatcac tcgaagaatt
     7621 gcaaatggaa accccattat agaatacgta gctaaaatcg aatccacacc agttgaaggc
     7681 gtagagatca agaaagagac cgaagcacct cgacttgctc cttctctgcg aagagcaatc
     7741 gccgtaggaa tcacgccaca agaacaaata gggagaggaa taccaaaaag tgtggcccaa
     7801 aggactgatt tgaaattggg ctttgaaatt ttaggaagat aaaatgactt aggtacccac
     7861 acatgtaaaa cacccgcgag taaaaagcct agtagtaaat agggagccat ttctacaaac
     7921 agcgaaatga aagactcgat aaagagagtc atgggagaac tcctttttta tgaagaatat
     7981 gatttaaacc cgtttgaaaa attaacccaa tatgctcatc atctagagaa taaaaaactg
     8041 tacgaccatc tcgacgcggg cgcactaaat gggaggttcg caaaagacgc aattgatggc
     8101 tcacagcgga ttgccctatg ttcagtttat cagcaatgtc atttacagac atttctccat
     8161 gaaggagtat ttcgataata cgaatgcgag tggtatcgct aaagaacttg aaaaattcag
     8221 ataattcaaa taagacatct agtgaaatct cttctttatt tgtttttcct ttacttgact
     8281 tcatttctca actctccaat atatgagcaa tggttcatat attcatatat acaaaacgaa
     8341 aaaagggttg tcaagatatt tttaaacaaa aaaaagagcc tgaaataaat caggcccttt
     8401 tttataaaca actagaggaa aattattcag cagctggtgc ttcttcagtc ttttccgctt
     8461 tttcagcttt tttctttgtt gtctttttct tcgctggagc aatagcttca ccgatgcttg
     8521 tgttattctc accaggcttg tgggagttca tccaagcttc gagttctgca gactcacgat
     8581 ttttgaagta atcaacaaca gaaagataga gcttacgatt attttgatcg atttctgtaa
     8641 cgattgcagg aatttcgtca cctactttaa atgcatctgc aggaaccttg atgtattctg
     8701 cagttaattt agagacaggg acaaaacctt cgatgccatc agccatttcg actacaacac
     8761 cgcgatcgag catacgaacg atagttcctt ttacttcagc atcaactggg tatgtagatt
     8821 caacagaatc ccatgggtct tctgtaagat gcttcataga aagagaaata cgacgttttt
     8881 cacagtctac agcaagaaca acacattcca ctttatcgcc cttcttgacg agttcgtttg
     8941 gatgagtgat tttcttagtc caagacatat cagaaacgtg aattaagcca tccacacctt
     9001 ccttgatttc aacgaaggca ccgaaagatg ccaaattacg gatttcgccg acaactcgtg
     9061 cacctggagg caattcagat tcgatggaat cccatggatc tgtttcaagt tgtttcatgc
     9121 cgagagaaat gcgttctgca tcttcttcaa ccttaagaac aacagcttcg acttcttgac
     9181 ctacaacgag gatttttgaa ggatgcttaa cgtgttgggt ccaagacatt tcagaaacgt
     9241 gaattaagcc ttcgatgccg ctatctaatt caataaatgc accgtagtca gtaatagaaa
     9301 caacttttcc atgaacggtc atgccttctg ggtagcgagc agcaacgtct ttccatggat
     9361 gtggtttcaa ttgtttcatg ccaagagaaa tgcgttcttt cttatcatta aagtcaagaa
     9421 ccatgacttc aacttcatta ccaagttgaa ccatttctgt tgggtggtta atgcgtttgt
     9481 agctcatgtc ggtaatatgg agaagaccat ctacgccgcc aagatcaatg aaggcaccga
     9541 agtcagtgat gttcttaacg atacctttgc gtacttgatt cttttcgaga gtttcaagta
     9601 catcaccgcg ttgacgattg cgttcttctt caagaactac gcgacgagag acaacaatgt
     9661 tacgacgagc cttgttgact ttgatgacct taagatcata gtcttggccg atgagagcat
     9721 tgatatctgg aatttgacga aggtcgattt gagagccagg aaggaaggca tcgattccga
     9781 agagatcgac aaccacacca cccttgatac gcttggtgag ggtaccacga acgacttcgt
     9841 tgttttcaaa agcttcatga atacgatccc aaacgcgaac aaagtcagct ttctgcttag
     9901 aaagaactaa acgaccgtca tcaccttcga gcttttcaac aaatacttca atttctgtgc
     9961 cgatttcaag agaatcggta tctttaaatt cgtcgcgagc aatcacgcct tcggacttaa
    10021 aattaacgtc aacgagaact tcttgttcgt taacttggct gatttttccg caaacgagtt
    10081 tacctggttc gaggcaatcc ataccggcat aaacgtcggc agcttctttg tgcccgatac
    10141 ttccaagttc agcttctgct tcgaggattt ctttcaggtc atcttgtgtt ccgtaaagga
    10201 ttttagacat atgtttcttt ttttggttgt tgtgcaaaaa atcaggttac gccactacac
    10261 tcacgatagc ctggattttt tccacttgtt cttggattga taattgtgta gtgtcaattt
    10321 ctatagcatc tggggctttt aaaagagggg ctacttctct agaagagtcg atagagtcac
    10381 gaagacgtag attttcttct acttcttgaa gagtgacttg tttgccttgc ccaatgagtt
    10441 cggcataacg acgagcagca cgtactttta gatctgtcac cagaaaaaac ttaaacgtcg
    10501 cgttagggaa aacaacggta ccgatatctc ggccatctag aatacaagaa tgcttgtttc
    10561 cgatttctct ttgttttgca gttagagccg accggatctc ggggacggca ctataatagc
    10621 tcacttgttc tgaaacttca gggctccgaa tagcggattc ctgagaaaac ccattaatta
    10681 aaacctgatt attggaatca aagtcaatca cggttttttg taataaactt aaaagagcct
    10741 ctttatctga aggagtaacg cctgaagtca tcgccacata ggtgattgcg cggtacatcg
    10801 ctccagtatc taaataagta atgcctaaat tttttgaaag caacttagct gtggtgcttt
    10861 tcccagtccc gctgggaccg tcaatggcaa taacaatatg atttttagac tctgacatga
    10921 ggccgctaat atagaaaagg acagaatacc tgtaaaacag aaaacctcgc ttttggcgag
    10981 gtttttgctt atcaacacta taaaatagac ttaatattcg gtatcttcgt aaaaatcaga
    11041 ggattccatg ttatcagaat cttcggtttc tagtacttct tctgcttctt gagtgttttc
    11101 atcggaatat tcttcaggag gaagaagttc ttttgtgcta ccatatttgc gtttttcttc
    11161 ttcggtcaat ttattagtga aaacttgcac ttctggttct tgaggtttgc tttcttcttc
    11221 aagaatcttt tggccttctt ctaggcgcct tttgatacgt tcttgttctc tcttttcgag
    11281 atccaagccc acctttgctt gatcttctga cattttataa cgttcgttag agtcgtccat
    11341 ggctttttta ataccaacga gacgaccaga accatcaacg tataattcca tttgttttaa
    11401 ggtagaaagc tttaagcgac gagccgctac ttggtattct ggcttaaatt ctttaccgtc
    11461 actaatggca taaacctcat cttctgtgac atatttaaga gcttcttccc aacggactcc
    11521 ttgtacactt gtagtgaagc caactaaagc gtcattgatt tgatcagggt tgctagactt
    11581 tgaacctgca cacccactta aaaccattaa aaatgcaaat aaaaccatca atcctttttt
    11641 cataggaata tcctccgaaa aattatccct tgctaaaata cattcatttt gttaatgaag
    11701 caattttttt aaacgaaaac gattctttac attccttatt ttctatcatt aaccgcctat
    11761 gaataccagt tctgatatct atcgcctaac tctccctgaa aacagagaga tcattttagt
    11821 gggtacggcc catatttcgc aagaatcgaa agatttggtt cgaaagacca ttgaagaaga
    11881 atcccctgac accgtttgtg tcgaattgga tgaagggcgt atgaaatcgc tcgaagagcc
    11941 tgatcgttgg aaaaaccttg atttgaaagc gattattcgc caaaaacaat tgggaacgct
    12001 tgtggcgaac cttgttttag ggtcttacca aaagcgcatg ggagatcaaa cgggagtacg
    12061 tcctggggcc gaactgcaag aggccgttca aattgctaaa gaaaaaaata tcccgattgt
    12121 actagccgat aggcaaataa aaaccacttt gcgtcgaaca tggtcttaca ctccttggta
    12181 tcgtaaattc gcccttttag gtgccctaat aagcagtatt tttgataaaa ccgaggtcag
    12241 tgaagaagaa cttcgaaaaa ttaaggaaca agattcttta aatgcaatga tggccgaatt
    12301 tggtaaaaca ttccctgaag tgaaaaaagt tttaattgac gaaagagacc aatttttggc
    12361 ctcacgaata atcaatgccc ctggtaaaaa agtcgtggct gtggttggtg caggtcatgt
    12421 gaaagggatt tctcaaatca ttgaacaaaa catggagttg ccttcggaag attctttaag
    12481 cgaaattaaa aaagcctctt tgacttttaa aatactcgct tggtctattc ctattgcgat
    12541 tgttgccgcc attgtagccg ttggattcca atcaggaatc gctagagcgg gcgaattaag
    12601 tttacattgg gccatgctca cgggtggagg cgccatgctt ggcactatta ttgctggcgg
    12661 tcacccttta acagtactta tcgccctact tacggcacct ataacaacac tcattccaat
    12721 gattggagtc ggtttcttta cggcactcac tcaagtgtat atgcgcccgc cacgcgttca
    12781 agaaatggaa actttaaatg atgatatttg gaaagtgaga cgctggtgga aaaatagagt
    12841 tacgcgcgtt ttcctttgct ttttattgcc tggactacct accgtaatag gaaaagtcct
    12901 tgccatctta aagatttatc aagcgttcta attattttca ggtaatttgc actttctgtt
    12961 cgttcgttaa ttattttcaa ttgagaatat catttataga caacatctaa aaatgctttg
    13021 gtaaaaggcc aaatcaactt ttaaacgttg cctaaaatca tttcaattat gaaacagata
    13081 cttagaatgg atagaaacta tttttaaagg ctcgctggaa tgaaaatccc atttaataaa
    13141 cctccctttg taggcactga gcttgattat ataaaacaag cggttcataa tggtcgaatc
    13201 tgtggtgatg gcgaatttaa tctcaaatgc cacgagtggc tagaaaacta ctctggatgt
    13261 gcgaaagctc ttttaaccac gagctgcaca catgctcttg aaatggctgc gatgctttgt
    13321 gatatcaagc caggcgatga aatcattatg ccttcattta cctttgtatc tacggcaagc
    13381 gcttttgtat taagaggtgc taaaattgtt tttgtagata ttcgccctga tacgatgaat
    13441 cttgatgaga cgaaactgga agacgccatt acgtctaaaa ctaaagccat tgtgcctgtg
    13501 cattatgcgg gcgtcgcttg tgagatggat actattttag cgattgcaga aaagcatggt
    13561 ttgtttgtga ttgaagacgc ggctcaaggg atgatggcaa gttataaagg gaaagttcta
    13621 ggaacattaa gtcactttgg ctgttatagt tttcatgaaa cgaaaaacta cagtatggga
    13681 gaaggcggag cccttttaat caacaaaaaa gattttgtga aacgagcgga aatcatccga
    13741 gaaaaaggca cgaatcgatg tgaatttcat cgaggagaag tggataaata cacttgggtg
    13801 gatttaggat ctagttattt gccaagcgaa ctcaatgccg cttatttgta tgctcaatta
    13861 ctcgaagctc aaaagatttt aagtaaccgc ttaaattgtt ttcagtttta taacgaacac
    13921 cttgagatac ttgaatgtcg tggcttgatt caacggcctc atattccagc agaatgtgaa
    13981 cacaatgcgc atatgtttta tatcaaagta gactctattg aaactcgcac ggctctactt
    14041 tctcatttga caaagaacgg aattctcgcg gtgtttcatt atgtaccttt gcatgtttct
    14101 ccagttggaa aaatccacgg acgctttcat ggcgaagatc aatataccac cagtgaaagc
    14161 aatcgtcttt tacgcctgcc cctctattac gggttacaag aaaaagatca agaatttgtt
    14221 gttcaaaagt tgaacgagtt ttttttaaga aaataatgct gttttttaga tcacctttac
    14281 ttatattcga tttattatcc aagagttata tttgcatacg gtaaattcaa aacgtcttct
    14341 tttactcggc gggagccatg ccgaaatccc gcttattaaa gccgcacaaa agcttggtta
    14401 ttatgtgatt accacaggca atgcgagaga cggtttaggg catcctgttg cagataaaaa
    14461 catattcgct gatttttctg ataaagaaca aatgctttcg attgctagag cagaaggtgt
    14521 ttctgcagta tgttctggat gtaatgattt tgctcttctc tccactgttt ttgtcactga
    14581 aaagctaggc ttaaaaggac acgattcctt aaaaatttca gagacgattc atcataaaga
    14641 tcgatttcgt gctcttgccg aaaagctcga gattgcttcc ccgaaagccg ttcaggtgaa
    14701 aagcattgag gatcttgaaa aagctcttct cacattaaaa tttcctctga ttattaagcc
    14761 tgtagattta actggtggca agggcgttcg acgcgtagaa acagcggaag aagccataga
    14821 tgcgtatcac catgcatttg aacgcactcg agaaaatcat attattgtag aagaatttat
    14881 tgaaggcaca aaccacgggt tctctgcttt tttagtagat caaaaagtgg ctttttattt
    14941 ttcagataac gagcaatact atttaaataa atatatggtt tctggagcca attctcctac
    15001 tattgtttgc gaatcctctt taaaacggct atgtacttat agcgaaaaaa ttgctcaaga
    15061 cttgcaactc gtggatggca ttttgcatat tcaatttata gagcacccca ctcttggccc
    15121 atatattatt gagatttgcc gtcgccctcc tggggatctt tacattcaat ttgtagaata
    15181 tgcgacaggc gtaccttacc ctgaatattt gattttagca gagacgggag ccgacgtcca
    15241 cagcataaaa caagctccca ctaagggttt ttggctcagg cactgcatta tggctaaaga
    15301 agaaggcgtt ttagaagacg tttattttgc tccagaaata aaaaataacg tttttgataa
    15361 attactctgg tataaaaagg gcgaaaaggt agaagacaaa ttatattata aagcaggcat
    15421 cgtttttcta aaattcgatt ctttaaaaga aatgcaagag aaaaccacct taatgaacga
    15481 atatattcaa tgcaaaataa accctatcaa atagccttta ttggaggcgg catcaattcg
    15541 gccgtcggtg agactcacaa aatcgcttct caaatggacg gttgttttca acttgtcgct
    15601 gggagtttca gttcgcacgc gaaaaccaat gaagaaacgg ctatcgcttg gaatgttcct
    15661 aaagaacgcc tttacgaaaa ctatttgact cttttagaaa aagaaaaagg gtgcctggat
    15721 gcggtggtga tactcgcgcc tacagaccat cataaagaaa ttgtgattca agcccttcgc
    15781 catggttacc ccgtcatttg cgaaaagtct cttgcgacaa gttatgaaga tggtcttgaa
    15841 ataaaaaaag tcgtagaaga aactcacgga tttttttgca ttacatacaa ttacactggc
    15901 tacccaatga ttcgtgaatt aaaacgtctc attaaagacg gtcgtttggg taccattcag
    15961 caaattcaaa tggagatgcc tcaagaggga tttttgcgcc ttggcccaaa tggcgagccc
    16021 ccaaaacctc aagcatggcg tttgcatgat acagtgattc caaaaatctc tctcgattta
    16081 ggaacgcatc ttcataatat gattttattt ttgacagaga aaaatccaca gcgaatcatc
    16141 tctgatcaaa cgacttttgg cttattccct caaattgtgg ataacgtttt tgctctcgcc
    16201 cagtacccag aaaatatgaa agctcaaatc tggtttagta aaactgcact agggcagcgt
    16261 aacgggcttc ggattcgcgt ttttgggaac aaagccagcg cggaatggtt ccaaatggat
    16321 ccagagacgc taaagtttgc ggatgtttat ggaaacatta gcttaatgga ccgcaccacg
    16381 caagttcaag aggcaaataa ggctcgctac aatcgtttta aggcagggca tcctgctggg
    16441 tttattgagg catttgcaaa ctattataga gatgttgctg attgccttac agagtataaa
    16501 aaaacgggcc aatttgaatc gccttttgtg tttggaatta agccgtcttg tgatgggctt
    16561 tccatgatgc aagcagcggc acggtctgct acaaacggtc agtgggagtt tgttttctac
    16621 gagtcgttgt agtttttttc attgtattgc gtctctattg catattaaaa actattataa
    16681 ttgattgaaa tggtattttc attaaaacaa ataaatggta ttgttgacgg taatgttttt
    16741 ttcattgcgt ctttatttaa tatcaaaacc actg
//
LOCUS       DAOOBB010000021        16275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_4657_length_16275_cov_4.427374, whole genome shotgun sequence.
ACCESSION   DAOOBB010000021 DAOOBB010000000
VERSION     DAOOBB010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 16275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16275
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4657_length_16275_cov_4.427374"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1388)
                     /gene="trpD"
                     /locus_tag="PLT31_01965"
     CDS             complement(<1..1388)
                     /gene="trpD"
                     /locus_tag="PLT31_01965"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HPW93931.1"
                     /translation="MIVMIDNYDSFTYNVVQAIARITTEPIEVLRSKECSIQDIESLK
                     PSKLIVSPGPGRPEDAGISVQAIQHFAGKIPILGVCLGHQAIGYAFGASIIQAKFIKH
                     GIAEEITLDGKGLFRTIGLKSTFTRYHSLVIDESTLSSDFEISARSSDGDIMGIRHKT
                     LEIEGVQFHPESIASVQGEAFFKAFLNYRRESLPVKQILNDLTSGKDLDKATAELFME
                     DLTEGVLDERQMSAILTAITSKGPAAAEIAGCAAVLCRKKKTMPLTDIELTDIVGTGG
                     DSKGSFNISSMSALIAATCGLPIAKHGNRAVSSKSGSADFYENIGFKIDVSPEKSAEV
                     IRKTNFGFLFAPVYHSAMRFAAPVRNVMGIKTIMNLIGPLSNPAGAAYQMLGVYSKDL
                     LLPVAEASKMLGAKRVMVVVSEDGFDEISPCAPTYVIEIDEKNQKKEYTINPSDYGIP
                     TCASSDLDGGTG"
     gene            complement(1385..2797)
                     /locus_tag="PLT31_01970"
     CDS             complement(1385..2797)
                     /locus_tag="PLT31_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HPW93932.1"
                     /translation="MTKRTDNIYLSLSGDRYTPFSLAKKLGAKAVLESASFSQGKDRY
                     SILMLEEAFRVIQDEEGVAFVVEGKRQPFHLKEKADILDALLEIAEENELPPQNIPLP
                     AAGIGYLSYEFCARCDTIHLAPQIDELHIPESEFIVGHVYIVFDHFTETLHVFALNYK
                     EHEIDLDNAILRLKEKLNDLDFSYMANPHNSSNCRVLTDLKESKKEYLEKVCELQKHI
                     VAGDIIQAVPSRRLQLESDVDALEVYRRLRSVNPSPYLFYLNYGTHQFIGASPESIVR
                     VRDGVASIRPIAGTRRRGKNEEEDLALKKELINDPKERAEHLMLVDLARNDLGRVCEA
                     GSVKLIRNMESEIFSHVIHLVSDVNGKVRKDKRPIDVLRSAFPAGTVSGAPKISAIEI
                     LSRLEKVKRNFYAGAVGYIEADGDLDFCITIRSALKQQNKWTLQAGGGIVYASEPERE
                     WEETNEKLGAMRAVLEGDLK"
     gene            3194..4654
                     /locus_tag="PLT31_01975"
     CDS             3194..4654
                     /locus_tag="PLT31_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HPW93933.1"
                     /translation="MSPFLIFFVLLGYFFLLGTISFFTSRNENDNDSFFIGAKKSPWY
                     IVSVGMIGATLSGISVVSVPGMVRSLNFTYMQMVFGFFIGYVVVVSVLLPVYYKLNLT
                     SIYGYLGNRFGSLTHRTGALFFIFAKAVGASARLYVVAIVLQEFIFDAWNVPFSLTIF
                     LTLFVIYLYSFNGGIKTIVWTDFFQTIALLLTVILMLVEVVRLLDFNVFSAVKVLVES
                     SYSQIFIFDDWHSRSHFIKQFLSGIFVVIVMTGLDQDIMQKNLSCKSYKESRRNMLWY
                     GFAFIPVNLLFLSLGGLLLIYASVNSIDLPAVNDSILPYFAIRLSPLVTVCFVIGIVA
                     AAFSSVDSALTSLTTSFFVDILKKDKQVDKKSRKKIHFGFTFLFFFLVLLFRFWNNQS
                     AIDTIYTIVSYTYGPLLGLFAFGLFTSYSIREKWVPWIAVASPLISYILKVILENQFG
                     YVFGYELLILNGLLTFIGLKLVAYKPKMNKEEKTLE"
     gene            4858..6570
                     /locus_tag="PLT31_01980"
     CDS             4858..6570
                     /locus_tag="PLT31_01980"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HPW93934.1"
                     /translation="MFESKTKNLNKIKLNLTIVGNLLLFELKKKAKQSRKAQEAVLRD
                     ILTISKDTEFGRKYQFERILSSVNDFSLMDLYEEFVPVSQYEDLYPYIERHKNGEENI
                     LFPGKPKMYATTSGTTNEPKWIPITEKYYHEVYNRMSPIWLYSFIAKRKHAFDGSIVS
                     NVGKAIQGAAPDGTVYGSIAGVTQRDCPPFIKAMFASPSDIFMIEDYKARYYAIMRMG
                     IERDVHLVIGGNPSTFIEMQNNVDEFLGEYIKDIEQGTLSQLFHIPEEVRQAVKPYLK
                     PNPKRANALREMKKNYPRLLPKHYWPNLQVLNVWKFGNSAVYFEKIKDFFPETCYFME
                     QGYFSPECRPGVVIDPDTDSTVIFPHKNYFEFVREKDIDKKKPKFFHLDELKVGERYC
                     IYVTTYSGLYRYNLNDLVEVTGFYEQTPMVRFVQKVNGIISMSGEKLNENQFISVVRQ
                     VEIEFSKKLLFFIGFASIKESRYHIYYEFQDDNIPDDLVTRFTARVDAILKSKNMEYA
                     DKRELGRVDAPIGHLLQIHAFEIFKSRIIDQGLYKDRFRLNYLMQDELRHGMFKKLIK
                     RKFS"
     gene            complement(6585..7310)
                     /gene="rph"
                     /locus_tag="PLT31_01985"
     CDS             complement(6585..7310)
                     /gene="rph"
                     /locus_tag="PLT31_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HPW93935.1"
                     /translation="MERIDNRAPNQLRTIRMTPHFISSADGSILIEMGKTRVICNATL
                     LPEVPKWLKGKGSGWITAEYALMPQSTSQRVDRERKGASGRTQEIQRLIGRALRGAAN
                     LSALGERAIVVDCDVIEADGGTRTASIIGGFVALAMALKKIKREAQIEEQILQHVITA
                     ISIGVVDGEPLLDLCYKEDSAADVDMNVVMRDASEFIEIQGTGEHSSFSRDTLNQLLD
                     WSEKACISIRELQLAILNHEKLP"
     gene            complement(7312..8178)
                     /locus_tag="PLT31_01990"
     CDS             complement(7312..8178)
                     /locus_tag="PLT31_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HPW93936.1"
                     /translation="MNRKKRIAFQGRHGAYSESAAYYLFGDNISLIPMHSFEDIYQGI
                     ETGLIDGGAIPIENSTAGSVYENFDLLYKWRHPIVAEVTLQIEHALCAIKGTRIEDLR
                     RVLSHPQGLAQCSRFFAQNPIIEKVPFFDTAGSAEEIAKRNNHTDGAIASAFAAQYYD
                     LEILANGIENMKGINFTRFYAIQKNQPELPEGDDVKTILLFELKKSKSSGSLYNALGC
                     FAHRDLNLTRIESRPHPDRPWEYIFHLSFEGSPHNPKVIEALQELQEYTDFIYRLGSF
                     KKGLTQGLKFEV"
     gene            complement(8171..8722)
                     /locus_tag="PLT31_01995"
     CDS             complement(8171..8722)
                     /locus_tag="PLT31_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HPW93937.1"
                     /translation="MPLRTGYDRKIGDYKPLPQSEVSTVAASSTKVTEEKNEETKTEI
                     IKEKTPKPTKEKTTQVANNSSFEEFIKPWIGTRYKFGGSSRSGTDCSGYVMQIYKTKL
                     NIALPHNAASMFKMGSAVKAADLKEGDLVFFGNVWGINHVGIYLSKNRFTHASSSQGV
                     TITPMEMDYWNSRYKGARRFINE"
     gene            9418..10764
                     /gene="hemN"
                     /locus_tag="PLT31_02000"
     CDS             9418..10764
                     /gene="hemN"
                     /locus_tag="PLT31_02000"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HPW93938.1"
                     /translation="MIETLLHYNINAPRYTSYPPANLFRKAENELEVKELWIASNALG
                     SSNLSFYFHIPFCSKRCLFCGCTSETIKEDSYREEYMQAIAKEMDKKLLWIDSSRVVT
                     QVHFGGGTPTSVPVCDLKFILDRLKEKFTFSAGAEIAIECNPASVTKELLSELALAGF
                     NRISYGIQDFNLETLRIIGRDPSLIDVPSLVSLSHELGFHSVNLDLIYGLPGQTLESF
                     SESVSKAIESKPDRIALFSYAHVPWKRPEQKHLESFVIPDAYHKMSFFLKAREMFREA
                     NYQSIGMDHFVIPTDSLYLAAKEGLLHRNFQGYCTRSTTGQVYAFGSSAISQLDNAYL
                     QNTHVTSDYIDKVKHEEMTEVRVHPLTETEKIWRNIIERLMCNNALKINEIEKKVLGS
                     RWDRLCALEKEGLLEKIADNEIRTTELGSLVIRYLVSVIDPLLENQKTEGVFSKTI"
     gene            complement(10757..11716)
                     /gene="argF"
                     /locus_tag="PLT31_02005"
     CDS             complement(10757..11716)
                     /gene="argF"
                     /locus_tag="PLT31_02005"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HPW93939.1"
                     /translation="MIDRKKHLLRLADWSEERILETIDIASRLKTEVHSGQYSDRFKG
                     QTLGMFFEKPSLRTITTFQVGIHQFGGFPVILDPDSIGLGKRESIKDVARCLSRWINA
                     LVVRCFKQELVDQLALYGDIPVINALTDDFHPCQAISFAQMFQEQIGSFQGKTIAFIG
                     DGNNVANSLLTLCAKLGMNFTLACPKGFEQPKWIIEESLPLLKKRGCQYRIFHNPEEA
                     VVDADILYSDVWVSMGQEGEKESKQSHFLPFQINDHLLTLAPEHCKVTHCLPAHRGEE
                     ITDSVMDGPKNLSYEEAENRLHAQKAILWQVMTATSDIKNSIK"
     gene            complement(11879..12865)
                     /locus_tag="PLT31_02010"
     CDS             complement(11879..12865)
                     /locus_tag="PLT31_02010"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPW93940.1"
                     /translation="MSILVTGGTGALGFHILSGVTRSGRKLYSYSDEQPQPWQKVPDV
                     NYLTGNLLNFKELFEVVQKIQPHYIYHLASQSSVGLSYKKPFETLSTNLLGTQNLLEV
                     VRQACPRAKVLLLSSSEIYGRTEEHLTTLHKETNPPNPLTPYASSKACMEILGNQFRN
                     AYGLHIVFVRPFHFTGPHHSRRFAIPSITYQLVKIKHYGNEPVLFTGSLDISRDVVDV
                     RDVARGMIQVLNGSESGSVYNICCGKSYTFRELTEFLIDIAGVNVDFRFDPSLERTND
                     IPLLIGDPTKIMDIGWKPLISIEDSLTDLFNEMVIRRRLELKMQAEENTKKA"
     gene            complement(12862..14487)
                     /gene="aroB"
                     /locus_tag="PLT31_02015"
     CDS             complement(12862..14487)
                     /gene="aroB"
                     /locus_tag="PLT31_02015"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HPW93941.1"
                     /translation="MYKHVYFTGFMASGKSRVGRHVAERLKRPFIDTDFLISEQANKT
                     ISEIFEQDGEPAFRKMELELIEKLAADPEPKVISLGGGALTQPSVVKAIRQSGVLIRL
                     WAEPDVLSERIGRKNNRPLMAGLDDTHRLEKVKKLLEEREIYYSKADFSVESSNEYPE
                     EHVTERVIRLLLFWESHVLNVETSSGERYPIFIGNNLLSKIGILLEGLQLMPSHHFLI
                     CSDNTVAKAQNQTVVQLRNQTAQSLVFKFRAGEINKNFNSLNQLYTFMLRRHFSRKTC
                     LLQFGGGVVGDMAGFGAATYQRGIPFIQLPTTLLSMVDSSVGGKVAVNHPEGKNMIGA
                     FYQPKAVICDLSVLSTLPHQEFLAGLAEIIKYGVIYDAAFFDFIESHVDSILNKDPAI
                     LKQLIQRSCEIKAEVVGIDEKETGLRAILNYGHTFGHAIEKLTDYKKFSHGIAVSLGM
                     RVAGRVSSLLQLWNPDDEKRQTDLLNRFGFPASFNVSAEEAWKAMSIDKKVDKNNRNY
                     ILPHKIGFVNKHTNIDEQIINNAWESIQAREEQ"
     gene            complement(14480..15385)
                     /gene="aroE"
                     /locus_tag="PLT31_02020"
     CDS             complement(14480..15385)
                     /gene="aroE"
                     /locus_tag="PLT31_02020"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HPW93942.1"
                     /translation="MTQINGKTKLLGVFGYPIEHSRSPVMQNALLKHLQINASYLPFS
                     FLPEHLSDAVQAFRTLGFIGANITIPFKEKIIPFIDELDSLSHFTGSVNTLYWKDGII
                     GSTLMGTTTDPYGAIFNLQNALGSSFQLDSSVNVALLGNGGAARAIAFVLAQKGVSLT
                     IVSRNQAKGEALVDDLTQATSTKPKTILFQDFESITEKIDLVINATSVGMSPSINETP
                     LLKNQIRSHQIIYDIVYNPPETLFLKEAKAIGCKVIQGTGMLAAQGAASFAKWFPAYS
                     TEENILKNALFMNNALIQDVEIPNV"
     gene            15882..>16275
                     /locus_tag="PLT31_02025"
     CDS             15882..>16275
                     /locus_tag="PLT31_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HPW93943.1"
                     /translation="MKLPVVSIVGRPNVGKSSLFNRILGRRAAVVSDRDGVTRDRHYQ
                     TANYKNREFIIIDTGGFLPDDTIDVLADSVRVQIFNAIQDSDLVLFMVDVRVGITELD
                     LQFSRMIKRLDKKVILVANKSEKESDRTE"
BASE COUNT         5051 a         2902 c         2938 g         5384 t
ORIGIN      
        1 ttacctgtac ctccatctaa atcacttgaa gcacaagtag gaattccata atcgctagga
       61 ttaatggtgt attctttctt ttgattcttt tcatcaattt ctattacata ggttggtgca
      121 caaggagata tttcatcaaa tccatcttca cttacaacta ccataactct tttggctcct
      181 agcattttag aggcctcggc aacaggtaaa agtaaatctt tagaataaac gccaagcatt
      241 tgataagcag cgcccgctgg gtttgataaa gggccgatta agttcataat cgttttaatc
      301 cccataacgt ttcggacagg agccgcaaaa cgcattgcac tatgataaac tggagcaaat
      361 agaaaaccaa aatttgtttt tcggatcact tcagctgatt tttcaggtga aacgtctatt
      421 ttaaaaccaa tgttttcata aaaatcggca gacccagatt tacttgagac ggcacgattc
      481 ccatgttttg caataggtaa tccacaggta gctgcaatta aagcagacat agaactgatg
      541 ttaaaacttc ctttactatc gccaccagta cccacaatgt ctgtgagttc tatgtcggtt
      601 agaggcattg tttttttctt tcggcaaagc acagcagcgc aacctgcaat ttctgcagcg
      661 gcagggcctt tagaggtaat ggctgttaaa atggcgctca tttgacgttc atcgagaact
      721 ccttctgtta aatcttccat aaagagttcc gctgtggctt tgtctaaatc tttgcctgaa
      781 gtcaaatcgt ttaagatttg ttttacagga agagattctc tacggtagtt taagaaagct
      841 ttgaaaaaag cttcgccttg cacagaggct atggattcag gatggaattg aacaccctct
      901 atttctagcg tcttatgacg aatacccata atatcgccat cactagatct tgctgatatt
      961 tcaaaatctg aagataacgt ggattcatcg atgactaagc tatggtagcg tgtaaaagta
     1021 ctttttaacc caatggttct gaataaacct tttccatcta aagtgatttc ttcagcaata
     1081 ccatgcttaa tgaattttgc ttgtataatc gaggctccaa aagcataacc aatagcttga
     1141 tggccgaggc aaacgcctaa gattggaatt ttacctgcaa aatgttgaat ggcttgaaca
     1201 gaaatgcctg catcttctgg acgtcctggg cctggtgata caatcaattt agaaggcttt
     1261 aaagattcta tatcttgaat agaacactct ttacttctta acacttcaat tggttctgta
     1321 gtaatgcgag caatcgcttg aaccacatta taagtaaaag aatcgtaatt atcaatcatg
     1381 acaatcattt taaatctcct tctagaacgg cacgcattgc cccaagtttt tcattagttt
     1441 cttcccattc acgttctggc tcagaagcgt agacaatacc tccgccagct tgtaatgtcc
     1501 acttgttttg ttgttttaag gcggatcgaa tagtgatgca aaaatcaaga tctccatcgg
     1561 cttcaatata accgactgct cctgcataaa aattgcgttt caccttttct aaacgtgaaa
     1621 gaatttcgat cgcactaatt tttggggctc cacttacagt tcctgcaggg aaagctgatc
     1681 ttaaaacatc aatagggcgt ttatcttttc tgactttacc attgacatca gaaacaagat
     1741 ggatcacgtg actaaaaatt tcactttcca tattgcgaat taatttaaca gaacctgctt
     1801 cacaaactct acctaaatca tttctcgcta aatcgacaag cattaaatgc tctgcgcgtt
     1861 ctttcggatc gttgatcagt tctttcttta aagcaagatc ttcttcttca tttttaccac
     1921 ggcgtcttgt tccagcgata ggtctaatcg aagccactcc atctcgaaca cgaacaatac
     1981 tttctggaga agctcctata aattgatgtg ttccgtaatt caagtagaaa agataaggtg
     2041 agggatttac tgaacgtaag cgacggtaga cttcaagggc atctacatca gattctaatt
     2101 gtaatcttct cgatggaaca gcctgaataa tatcaccagc cacaatatgt ttttgtaatt
     2161 cacatacttt ttcaagatat tctttcttag attcctttag atccgtaaga actcggcaat
     2221 tagaactatt atgagggttt gccatataag aaaaatcaag atcattcagt ttttctttaa
     2281 gtcttagaat cgcattgtca agatcaattt catgttcttt ataattgaga gcaaaaacat
     2341 gaagagtttc tgtaaagtga tcaaaaacaa tgtaaacatg cccaacaata aattcagatt
     2401 ctggaatatg caattcgtct atttgaggag ccaaatgaat ggtatcgcaa cgagcgcaaa
     2461 actcataact gagataacca attcctgcgg ctggtagcgg aatattttgt ggtggaagct
     2521 cgttttcttc tgcaatttcg agaagagcat ctaaaatatc cgccttttct ttcaaatgaa
     2581 aaggctgcct tttcccttcg acgacaaagg caaccccttc ttcatcttga atcactcgaa
     2641 aagcctcttc gagcattaaa atggagtagc gatctttgcc ttgagaaaaa gaggctgatt
     2701 caagaaccgc ttttgcaccg agctttttgg caagagaaaa tggagtgtaa cgatctccag
     2761 agagagagag gtaaatgttg tctgttcgtt tagtcatcat tcatccgttg ttaaaaaaaa
     2821 tcaagaaata aaaaaaggct ccggggagtc cggagccaga tttgaaaaaa aacaatccaa
     2881 gcacaaaaac cagactccag tctactgagt ctgccaccac caattgttat ttgtgcaaat
     2941 aaaattcatg tgcataaaac taagcataat taaaatgatt gtcaagtaaa tttttaattt
     3001 ttttttgtag cctcatttat gtggtaaaaa cacaaaaaat gaaaaacttc catatatatg
     3061 gattcaatta caaaaatgca catttttatt gaattttaat ttttatcaga gccctttttt
     3121 aggggcgggt tctagtaagc agtgtttctt aaaagttttt ctacttttga aattctacat
     3181 agaggaataa ttaatgtctc cttttctaat attttttgtc ttactaggct acttcttttt
     3241 attaggaacg atttcatttt tcacaagtcg taatgaaaat gataatgatt ccttctttat
     3301 tggagcgaaa aagtctcctt ggtatattgt ttcggttggg atgattgggg ctactctttc
     3361 tggcatttca gtggtctcag ttcctggaat ggtgcgttcc ttaaatttca cttatatgca
     3421 aatggttttt ggatttttca ttggatatgt tgttgtggtt tctgttcttt tacctgtgta
     3481 ttataagcta aatcttacaa gtatctatgg ttatttgggg aatcgttttg gttctttaac
     3541 tcatcgaaca ggggcattat tctttatctt tgctaaggcc gttggtgcat ctgcaaggct
     3601 ttatgtagtg gctattgtat tacaggaatt catttttgat gcctggaatg tccctttttc
     3661 gcttacgatt tttttgactc tttttgtgat atacctttat tcgtttaacg gagggataaa
     3721 aacaattgtt tggacagatt tttttcagac gattgcctta ctattaactg taatcttaat
     3781 gcttgtagaa gtagttcgat tactcgattt taacgtattt tctgcagtga aagtattagt
     3841 agaaagttct tattcacaaa tctttatttt tgacgattgg catagtcgaa gtcattttat
     3901 aaaacaattt ttgagtggta tttttgtggt gattgtcatg actggtttag atcaagacat
     3961 tatgcaaaaa aacttaagtt gtaagagtta taaagaatca cgtcgaaata tgctttggta
     4021 tggatttgct tttattccag tgaatttatt gttcttatct ctgggtggtc tgcttttaat
     4081 ttatgcttcg gtaaattcta ttgatttacc tgcagtgaat gattcgattt taccttattt
     4141 tgcgattcgt ttaagtccct tggtaacggt ctgttttgtg attggtattg tggccgcggc
     4201 tttttcgagt gtggattccg cattaacatc attaaccacc tcattttttg tagatatctt
     4261 aaaaaaggat aagcaggtag ataaaaagtc tagaaaaaaa atccattttg gttttacttt
     4321 tttgtttttc tttttagtcc ttttatttcg gttttggaat aatcaaagtg ccatagacac
     4381 catttatacg attgtttctt atacttatgg acctttatta gggctgtttg cttttggcct
     4441 ttttacttcc tattctattc gtgaaaaatg ggttccttgg attgctgttg cctcaccttt
     4501 gatttcatat attttaaaag tgattttgga gaatcaattt ggttatgttt ttggatatga
     4561 attattgatt ttaaatggtc ttcttacttt tataggttta aaattagttg cttataagcc
     4621 aaaaatgaat aaagaagaga aaactctcga gtaaaaatgt tattattaac taactattca
     4681 ttaaaattag tgtggttgat gatgaaaacg ggtaaacttt agtgattgta aacggaattc
     4741 agttttaaaa aaactattcg atgtatttta atgtttaatt atttaaacat ttgtcttttt
     4801 gtctatttaa tgaataaatt ctgattaaaa ttgggattat tttactttct ttcctatatg
     4861 tttgaatcga aaacaaaaaa cttaaataag ataaaattaa acctaacaat tgttggaaac
     4921 cttcttttat ttgaattaaa aaagaaagcg aaacaatcta gaaaagctca agaagcggtt
     4981 ttacgagata tattaacgat ttcaaaagat accgagtttg gtcgaaagta tcagtttgag
     5041 agaattctat cgtcggtgaa tgatttctca ttaatggatc tttatgagga atttgttcca
     5101 gtaagtcaat acgaagattt atatccttac attgaacggc ataaaaatgg agaagaaaat
     5161 attctttttc ctggaaagcc taaaatgtat gcgactacaa gtggcaccac taatgaacct
     5221 aaatggattc caattacaga aaaatattat catgaagttt ataatcggat gagccctatt
     5281 tggctttatt cttttattgc caaacgtaaa catgcttttg atggttctat cgtatccaat
     5341 gtagggaaag caattcaagg ggctgctcca gatggcactg tttatggatc aatagcgggt
     5401 gttactcaaa gagattgccc tccttttatt aaagctatgt ttgcctcgcc atcagatatt
     5461 ttcatgattg aagattataa agctcgttat tatgcgatta tgagaatggg aattgagcga
     5521 gacgttcatt tagtgattgg cggtaatcca agtacattta tagaaatgca aaataatgta
     5581 gatgaatttc ttggtgaata tattaaagat atagaacaag gtactttaag tcaattattt
     5641 catattccag aagaggttcg acaagcagta aaaccttatt tgaaacccaa tccgaaacgg
     5701 gctaatgctc ttcgagaaat gaaaaagaac taccctcgat tacttccaaa gcattactgg
     5761 ccaaatctgc aagttttaaa tgtctggaaa tttggaaatt cagctgttta ttttgaaaag
     5821 attaaggatt ttttccctga gacgtgttat tttatggaac agggttattt ctctccagaa
     5881 tgtcgtcctg gtgtagttat tgatccagat actgatagta cggtcatatt tcctcataag
     5941 aattattttg aatttgttcg agaaaaagat attgataaaa aaaagcctaa atttttccac
     6001 ttagatgagt taaaagttgg ggaacgttat tgcatttatg tgacaacgta ttcgggcctt
     6061 tatcgttata atctaaatga cttagtcgaa gtgactggat tttatgagca aacaccgatg
     6121 gttcgttttg ttcaaaaagt taacggtatt atttcgatgt ctggtgaaaa attaaatgaa
     6181 aaccaattta taagtgttgt tcgacaagtg gaaatagaat tttctaaaaa acttctcttt
     6241 tttattgggt ttgcttctat taaagaatct agatatcata tttattatga atttcaagat
     6301 gataatatcc cagacgattt agtgactcgt tttaccgcaa gagtagatgc catattaaaa
     6361 tctaaaaata tggaatatgc agacaaacgc gagttaggcc gtgtcgatgc tccaattggg
     6421 catcttttac aaatacacgc atttgaaatt tttaaatcaa gaattattga tcaaggcctt
     6481 tataaagacc gtttccgatt gaattatttg atgcaagacg aattacgtca tggcatgttc
     6541 aagaaactaa ttaagcggaa attttcctag agaaacctta aatattaagg tagtttttca
     6601 tgatttaaaa tggctagctg cagttcccta atagaaatac aagctttttc actccaatct
     6661 aatagttgat ttaatgtgtc tctactgaaa gaggaatgtt caccagtgcc ttgaatttca
     6721 ataaactcag aagcatcacg catcaccaca ttcatatcga catcggcagc agaatcttct
     6781 ttatagcata aatccaataa aggttctccg tccacgacac caatagaaat agctgtgatc
     6841 acatgttgta gaatctgttc ttctatttgg gcttctcgct taatcttttt taacgccatc
     6901 gcaagtgcta caaaaccacc aataatactt gctgttcttg taccaccatc agcttctatc
     6961 acatcgcaat ccactacaat agcacgttct ccaagtgcag aaagattcgc tgctcctcta
     7021 agagctcggc ctattaatct ttggatttct tgagtccttc cacttgctcc ttttcgttct
     7081 ctatcgactc gttgagaagt actttgtggc attaaagcat attccgctgt aatccaacca
     7141 gatccttttc cttttaacca tttgggcact tctgggagta aagtggcgtt acaaatgaca
     7201 cgtgtttttc ccatttcaat taatatagaa ccgtctgcac tactaataaa gtggggtgtc
     7261 atacgaatgg tgcgtagttg atttggtgca cgattatcaa ttctttccat tttaaacctc
     7321 gaattttaaa ccttgtgtta atcctttttt aaatgaacct agccgataaa tgaaatcggt
     7381 gtattcttgt aattcttgaa gggcttcaat gactttaggg ttgtgaggag atccttcgaa
     7441 agaaagatgg aaaatatatt cccaaggcct atctggatga ggtctacttt cgattcgagt
     7501 caagtttaaa tcacgatgag caaaacaacc aagagcatta taaagagaac cagaagattt
     7561 agatttcttt agttcaaaaa gcaaaatcgt tttgacatca tctccttcgg ggagttctgg
     7621 ttgatttttt tgaatggcat agaaacgtgt aaaattaatg cccttcatat tttcaatccc
     7681 gttcgcgagg atttctaaat cgtaatattg agccgcaaag gcacttgcta tagcaccatc
     7741 ggtgtggtta tttctttttg caatttcttc agcactgcca gcagtatcaa aaaaaggaac
     7801 tttttcaata ataggatttt gagcaaaaaa gcgactgcat tgagcgagcc cttgaggatg
     7861 actaagaact cttctaagat cttcaatgcg tgttccttta atggcacata gtgcatgttc
     7921 aatttgaaga gtcacttctg ctacaattgg gtgtcgccat ttgtataaaa gatcgaaatt
     7981 ttcatataca gagccagcag tagagttctc tataggaatt gcaccaccat caattaggcc
     8041 tgtttctatc ccttggtaaa tgtcttcaaa ggagtgcatg ggaatcaaag aaatattatc
     8101 cccaaacaaa taatacgctg cactttcgct ataggcacca tgccttccct gaaaagcaat
     8161 tctttttttt ctattcattt atgaacctcc tggccccttt atatcttgaa ttccaataat
     8221 ccatttccat tggagtaata gtcacgcctt gagaagaact cgcgtgagtg aagcgattct
     8281 tgctcaaata aatacccaca tgattgattc cccaaacatt gccaaaaaag acaaggtctc
     8341 cctcttttaa atccgctgct tttacagcac ttcccatttt aaacatgcta gcggcgttgt
     8401 ggggaagagc aatatttagt tttgttttat aaatctgcat tacataacca gagcagtctg
     8461 tgccagaacg ggatgaacca ccaaatttat agcgtgttcc aatccatggt tttataaatt
     8521 cttcaaatga cgaattatta gcgacctgag ttgttttttc tttagtcggt ttgggtgttt
     8581 tttctttgat tatttccgtt ttagtctctt cattcttttc ttcagttact tttgtagaag
     8641 acgctgctac ggtagagact tctgattgag gaagaggctt ataatcgcca atcttacgat
     8701 cataacctgt tctgagaggc attgtacaag cagataaaaa taaaacagaa taaataagcc
     8761 ctttttttag aaaagagggc gaaaacaata aacaaaagat cgattgattc cttttcaaca
     8821 cattcagggg caaatcaatt gggtaaaaac gacgcattaa taacaattta gaaatatata
     8881 tgctaaaaat tgttgtatca tttcatttat tgcccaattt ggaatgattt attatatcat
     8941 agaataacgc tatgaagagg tgtgattcat attattgaaa gctgcttcgc gttcgattcc
     9001 cctctaaaac agcccagttt ttttatgccg aatcaagcgt tatttgatcc cataaaggga
     9061 tcactaccac aggggtatca ttctcactaa atgctaaagc ataagtgttc atgtttctca
     9121 cataaaaagt aaacttttaa gtgttcatgt ttgtggcaca caacagagtg aggaggtaat
     9181 accatttccg tcaacaatat catttattaa taagttttaa tgaaaaaaaa cattgccgtc
     9241 aacaatggtg ttattcataa aaaacgaaat aaaatcaacc cgagcgaaga gataacagtc
     9301 atttcaatca attataatag tttttgatat gctatagaaa tgtcatgaaa aaacaatcac
     9361 gtcgttaatt taattaaagc gaataattgc ataaaagctt ttttctatat taaacgcatg
     9421 attgagacgc ttctacatta taatataaat gctccccgat ataccagtta tccacctgcg
     9481 aatcttttta gaaaagcaga aaatgaattg gaagttaaag agctctggat tgcttctaat
     9541 gcattaggat catcgaattt aagcttttat tttcatattc ctttctgttc aaagcgttgt
     9601 cttttttgtg gctgtacttc tgagacgatc aaagaagact cttatagaga agaatacatg
     9661 caagccattg caaaggaaat ggataagaaa ttactttgga tagactcttc tagagttgtt
     9721 acccaagtgc attttggggg tggaacaccg actagcgtac cagtctgtga tttgaaattc
     9781 attcttgata ggttaaaaga aaaattcaca ttctctgcgg gagctgaaat tgcgatagaa
     9841 tgtaatcccg cctcagtaac aaaagagctt ctttctgaat tggcgttagc gggttttaat
     9901 cgaattagtt atggtattca agattttaat ctagaaacgc ttcgtattat aggtcgtgat
     9961 ccttctttaa ttgatgttcc atctcttgtt tctctttcac atgaattagg ttttcatagc
    10021 gtgaatctcg atttgattta tggattacca ggtcaaacgc tagagagctt ttctgaaagt
    10081 gtttctaaag ccattgagtc aaaaccagat cgaattgctc tttttagtta tgcgcatgtg
    10141 ccttggaaac gcccagaaca aaaacattta gaatcatttg ttattcccga tgcttatcat
    10201 aaaatgagtt ttttcttaaa agcacgtgaa atgtttcgag aggcaaacta tcaaagtatc
    10261 ggaatggatc attttgtgat accaacggat tctttatatt tggcggcaaa ggaaggtctt
    10321 ttacatcgta attttcaagg ctattgcaca agaagtacta ctgggcaagt ttatgcattt
    10381 ggttcaagtg cgatttctca gctggacaat gcttatcttc aaaatacaca tgtcacctca
    10441 gattacatag acaaagtaaa acatgaagaa atgactgaag tgcgagttca tcctttaacg
    10501 gaaacagaga aaatatggcg caatatcatt gagcgattaa tgtgtaacaa tgcgctaaag
    10561 atcaatgaaa tcgaaaagaa agttcttggt tctcgttggg atcgtctttg cgcacttgaa
    10621 aaagaaggct tactagaaaa aatagccgac aatgaaattc gcaccacgga actaggttct
    10681 ttagtgattc gctatttagt atctgtgatt gatcctttac ttgagaatca aaaaacggaa
    10741 ggcgtattca gcaagactat ttgatagagt ttttgatatc gctggtggct gtcatgactt
    10801 gccaaagaat cgctttttgt gcatgtaagc gattttctgc ttcttcataa ctcaaattct
    10861 taggcccgtc catcactgaa tcagtgatct cttcgccacg gtgtgctggc aaacagtgag
    10921 tgactttaca atgttctgga gctaatgtaa gcaagtggtc attaatttga aaaggaagaa
    10981 aatggctttg cttagattct ttttcgcctt cttgccccat actgacccaa acatcgctat
    11041 ataaaatatc tgcatcgaca acagcttctt ctgggttatg gaatatacgg tattgacagc
    11101 cacgcttttt aagaagagga agagattctt ctataatcca tttaggttgt tcgaaacctt
    11161 taggacaagc taaggtaaag ttcattccta gtttagcaca caatgttaaa agggagttcg
    11221 ccacattatt gccatcacca atgaaggcaa ttgttttccc ttgaaaacta ccaatttgtt
    11281 cttgaaacat ttgagcaaat gaaatggctt gacatgggtg aaaatcgtct gttaaagcgt
    11341 taataacagg aatatcacca tatagagcga gttgatctac taattcttgc ttaaaacaac
    11401 gaactacaag tgcattaatc caacgagaaa gacagcgagc cacgtctttt atgctttcac
    11461 gctttccaag tccgatagaa tcaggatcta gaattacagg aaaaccacca aattgatgaa
    11521 ttccaacttg aaatgtggta attgttcgta aagaaggctt ttcaaaaaac attcctagtg
    11581 tctgaccttt aaaacgatca gaatactgcc ctgaatggac ttctgttttt aagcgtgaag
    11641 caatatctat tgtttcgaga attctttctt cactccaatc agcaagccga agtaaatgct
    11701 tcttacgatc gatcatgtta gttctttgat acctcaaagt ttaaactttt aaactatttt
    11761 agcgtttttg ttatttaaaa aagaaaaagg ctgtttgaaa cgatcaaacg ccattctctt
    11821 gtagagaaat cagaatatat attctttttc ttaaaaaggg aagaaaaata ttaaaaaatt
    11881 atgctttttt agtattctct tcagcttgca tttttaactc taaacggcgt cgaatcacca
    11941 tttcattaaa taaatcggta agactgtctt caatactaat tagaggtttc cacccaatat
    12001 ccattatttt agttggatct cctatcaata aggggatgtc gttggtgcgt tcaaggcttg
    12061 ggtcaaacct aaaatcgaca ttaactcctg caatatcaat taaaaattca gtcaattctc
    12121 taaaagtata agattttcca cagcaaatat tataaactga accagattct gaaccattta
    12181 gaacttgaat cattcccctt gctacgtcgc gaacatcgac tacgtcacga ctgatatcta
    12241 gacttccagt aaataaaacg ggttcgtttc catagtgttt tattttcacg agttggtatg
    12301 taattgaggg aattgcaaaa cgtctactat ggtgaggacc tgtaaaatga aatggccgaa
    12361 caaaaactat atgtaatcca taagcatttc taaattgatt acctagaatt tccatacatg
    12421 cttttgatga cgcataaggc gttagtggat taggtggatt cgtttcctta tgtaaggttg
    12481 ttaaatgctc ttcagtacgc ccataaattt cactggaact taaaagaaga actttcgccc
    12541 gaggacaagc ttgacgaacg acttcaagta ggttttgagt cccgagaagg tttgtactca
    12601 acgtttcaaa aggctttttg taagaaagtc caacggaaga ttggcttgct aaatgataaa
    12661 tatagtgagg ttgtattttc tgcactactt caaaaagctc tttaaaattc aataaattac
    12721 cagtcaaata attaacgtct gggacttttt gccatggttg aggctgctca tcgctataac
    12781 tgtataattt acgaccggag cgtgtgactc cagataaaat gtgaaaccct aaggctcctg
    12841 taccaccagt tacaagaata ctcattgttc ctctcttgcc tggatagatt cccaggcatt
    12901 gttaatgatc tgttcgtcaa tattagtgtg tttatttaca aaacctattt tatggggaag
    12961 aatataattt cgattatttt tatccacttt tttatcaata ctcatagctt tccaagcctc
    13021 ttctgcggaa acattaaaag aagctgggaa accaaatcga tttaagagat cggtttgacg
    13081 tttttcgtcg tcaggattcc atagttgtaa aagagaagaa actctaccag cgactctcat
    13141 tcctaaacta acagcaatcc cgtgagaaaa ctttttgtaa tctgtaagtt tttcaatggc
    13201 atgaccaaaa gtgtggccat aatttaagat ggctctaagg ccagtttctt tctcatctat
    13261 tcctacgact tctgctttga tttcacagct tcgttgaatg agttgtttta aaatagcagg
    13321 atctttattc agaatagaat ctacatggct ttcgataaaa tcaaaaaagg ctgcatcata
    13381 aataacacca tatttgatga tttcagcaag tcccgctaaa aattcttggt ggggaagagt
    13441 agataaaaca gataaatcac aaatcacggc tttcggttga tagaaagccc ctatcatgtt
    13501 tttcccttct ggatgattta cagccacttt tccaccaaca gaactatcca ccatacttaa
    13561 aagcgtagta ggaagctgga taaatggaat ccctcgttgg tatgtggcag caccaaatcc
    13621 tgccatatct cctacaacac caccaccaaa ttgaagaaga catgtctttc tactaaaatg
    13681 acggcgaagc ataaaggtgt aaagctgatt tagactatta aaatttttat taatctcgcc
    13741 agctctgaat ttaaaaacaa gggattgtgc tgtttggttt cttagttgta caaccgtttg
    13801 attttgggct ttagcgaccg tgttatctga acaaattaaa aaatgatgcg aaggcattaa
    13861 ttgaaggcct tcaagaagta tccctatctt tgataacaag ttatttccta taaaaatagg
    13921 gtagcgctct ccagatgacg tttccacgtt taatacatga ctttcccaaa aaagtaaaag
    13981 acgaatcact cgttctgtga cgtgttcttc tggatactca ttagaacttt cgacactaaa
    14041 atcagctttt gaatagtaaa tttctcgttc ttctagaagc ttttttactt tttctaatcg
    14101 atgagtatca tcaagaccag ccatcaatgg acgattattt tttcttccaa ttcgttcaga
    14161 taaaacatct ggttctgccc ataagcgaat taaaactcct gattgacgaa tggctttcac
    14221 aacagagggc tgagttaagg ctccgccacc taaagatatt actttaggtt cagggtcagc
    14281 cgcaagcttt tcaattagct ctagttccat tttccgaaaa gcgggttcac cgtcttgttc
    14341 gaatatttct gaaatagttt tgtttgcttg ctcagaaatt aaaaaatcag tgtctataaa
    14401 aggacgtttt aaacgctctg caacatggcg tcccacacgg cttttaccac tagccataaa
    14461 tccagtaaaa taaacatgct tatacattag gtatctccac atcttggatt aaggcgttat
    14521 tcataaataa agcattctta agaatatttt cttctgtaga atatgcagga aaccactttg
    14581 caaaactagc cgcgccttga gcggcgagca tacctgttcc ttgaatcact ttgcaaccaa
    14641 ttgcctttgc ttctttcaaa aaaagcgttt caggaggatt atatacaata tcgtatatga
    14701 tttggtgaga gcgtatttga ttttttaata aaggagtttc attaatggaa ggggacatcc
    14761 ccacagaagt ggcgttaatt actagatcta ttttttctgt aatagattca aagtcttgaa
    14821 ataaaatagt ttttggtttc gtgctcgtcg cttgagttag atcatctact aaagcttcgc
    14881 ctttcgcttg atttcttgaa acaatcgtta aagaaacgcc tttttgagct aaaacaaagg
    14941 caatggcacg tgccgcaccg ccattgccaa gaagagcaac atttacagat gaatctagtt
    15001 gaaaagagga tcctagtgca ttttgaagat taaaaatggc tccataaggg tcggtggtcg
    15061 ttcccatcaa agttgaacca ataatgccat ctttccaata aagagtattc acgcttccag
    15121 taaaatgaga taaagaatct aattcatcaa taaaagggat gattttttct ttaaaaggaa
    15181 tggtgatgtt tgctccaata aaacctaatg tacgaaaagc ttgaactgca tctgaaaggt
    15241 gttcgggtaa aaaagaaaaa gggagatagg aggcattaat ttgcagatgt tttagtaaag
    15301 cattttgcat tactggggat cgagagtgtt caataggata tccaaaaacg ccaagcaatt
    15361 ttgttttgcc atttatttgc gtcacatagt cctctgtttt ttaatggagc atctccaact
    15421 ttaaggaatc tctcaaaaga cattatcaat attttgaaaa ataacttatg tgaaaaggat
    15481 atttttgcca aaagaagcgt tgtcatgtat ttctaggctt tttggccaat tttccagtaa
    15541 tcacttggct atttcacaaa actatctacc aaagtaattt tcagagattc catttagttt
    15601 ttacactaat tggaatgatc caagatttat ttcctcttaa aaatacatat aaaaaagtct
    15661 cataaatctt aaaaactgaa ttgttttgct ttttttacct cattttaagc ttttttaaag
    15721 atagatatgc ttttcttcac aatggatact cagcaaaaag tcttacaaaa tgagcaataa
    15781 aaaatgattt tccctgatta aaaaggttat agtagagaaa cctcttattt ttatactcca
    15841 aataacgctt tttctaaatc cttttctata ttagcggcgt gatgaaatta cctgtagtat
    15901 ctatcgttgg acgcccaaat gtggggaagt cctctctttt taaccgaatt ttaggtcgcc
    15961 gtgctgcggt ggtatctgat cgtgacggag tcactcggga tcgccattat caaaccgcga
    16021 actataaaaa tcgtgagttt atcatcattg atacaggtgg ttttttgcct gatgatacga
    16081 ttgacgtatt agcagatagt gttcgagtgc aaatttttaa tgccatacaa gattccgatt
    16141 tagtcttatt tatggtggat gttcgagttg gcattactga gttggattta caattttctc
    16201 gaatgattaa gcgtttagac aaaaaagtca ttttagttgc caataaaagc gaaaaagagt
    16261 cggatcgtac agaaa
//
LOCUS       DAOOBB010000022        16053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_4749_length_16053_cov_5.298162, whole genome shotgun sequence.
ACCESSION   DAOOBB010000022 DAOOBB010000000
VERSION     DAOOBB010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 16053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16053
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4749_length_16053_cov_5.298162"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..1220)
                     /locus_tag="PLT31_02030"
     CDS             complement(69..1220)
                     /locus_tag="PLT31_02030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPW93944.1"
                     /translation="MRKIIKVSIILVILATLILGARFIFFNTKESSVGVFVSAAVEQD
                     TLLLTSISATGSLEPVDRVEIGTQVSGDLSRIYVDFNSKVKKGQVLAELDKSKWITNL
                     RQSELAYKAAENDYQYKKNIFERTQKLAESKSVSAVELETAEYNMNAARFSMERSKSD
                     VQQAKLNLSYCVIKSPIDGVVLERSVDVGQTVAASMSAPVLFILARDLSQMRVMASVD
                     EADIGSILQGQRVEFTVDAFPKDRFFGKVEELRLNPTVTSNVVTYTVVISAENPEFKL
                     LPGMTATCTIVTKEEPNAMVIPLQALQFKPAEGSYEKGMPRGQGQRSISGKGKKGFGR
                     SDDAARVWVSVNGRPEPRRIKTGINDGVRVQVLEGLSVGDSVIVYVFLF"
     gene            1504..2949
                     /locus_tag="PLT31_02035"
     CDS             1504..2949
                     /locus_tag="PLT31_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPW93945.1"
                     /translation="MKKALLLLIPSFLFALQLPVHKEVLDNGLTVLLHPNKQSPTVSC
                     RLFYVTGSVHEVPGSSGLAHMLEHELFKGTRKVGITDSIADSVFMYKQDSLQALMRPL
                     IQKGDTTHYLKLKAEHDSIVNEHRKIFVKDELWSTYQAAGGTGLNAFTSDLMTAYIVT
                     LPKNKVELFLWLEADRMQNAILREFYSERDVIREERRMRYDDRPTGRFYETLNSMIYE
                     AFPYRIPTIGWPSDIENLTREKAAEHYHKYYKPRNAILVLAGDLDTAEVMPLIKKYFG
                     SIPPGEPFPAITVRDPEQAGEKRLIVRRQDAPHLLTIVFKTPEVGDSKLYALDIAEGV
                     LNGRSGRLHKRLVQEENLAVKVSASNSPNKYVSEFSIQAELEPDADPAKAEAIIWEEL
                     EKLKVELLSQRDFQKVKNRAYASLVRSLTDMENVATMLAWYEMYGDYHIFLNWANALE
                     KVTAQEVQKAAQETFSRNKATIGILTKENAK"
     gene            2946..4523
                     /locus_tag="PLT31_02040"
     CDS             2946..4523
                     /locus_tag="PLT31_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPW93946.1"
                     /translation="MKWISPLNKFPSKKSSTTPSKAIFKESLMPFKMTLFFLILLIFG
                     CSSTPVKNNVSEVTPVSKTNEEQVVSKSTNKALPSSYKEISFPEFTYVAPHPKEYRVQ
                     LDDSITGYVVVDRSLPLISFSMFFKEATIPSNIKETAALELLSPMFRRGGSSDISANA
                     LDDSLEFISASIQGGLGSYYSKISIDCLSKNFEEMLSLSKSVYTKPGFDEKQLEIQKT
                     SYITAYKRRFDNPASILSALRSKVNYASSPRLWNPTIEEYQNVSKKDLERFAKTKFSA
                     NRIVFALAGDIEKDSALVLLKKYFADWKKTTSREPTPSMALLKKPGVYAVEKDITQAN
                     ISINQPFVKRPHPDYYPTAVASFILGGGSFSSRLTSKIRSEEGLAYSVYSHAGNDYND
                     TALTTIALQTKAESAFFAVQLIIQEIQKLSKEGPTLEELELAKKTLIESLHGLFDSPS
                     STASIFAVDELVGKSQDHYLEYVQEINAITPEQVKAMTAKYFDPQKMTISIVGPKKSW
                     DKFENVTIIPIQDLYFR"
     gene            4536..5426
                     /locus_tag="PLT31_02045"
     CDS             4536..5426
                     /locus_tag="PLT31_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93947.1"
                     /translation="MRFLLFLFILLSTTPLIMAYDQKSMCSSAKQCLKLDIAGCSAED
                     KKPNSKIKYNDDFCAPYIELKKRGVSLETEISKEIFGRLGSQYRAIYINEGVLQTTPG
                     MMSYLFDHMSFTAKLINAYQGGAYSIDYKTPNRRVFSGTNGRSLSGDFIWVAQDSAGQ
                     KLGFRNVFFGVGRVKILRWHLTGTAIAFLDMDSIGKNQVRYKLTAIVFPGNAVLNSIM
                     HMDAFKNIVAEKINLIIKDIQESSSRYAKGDKKPIGQSKALQTKNELIHLKEFESVVQ
                     GTPWTLGDYFKKKGSYVKPK"
     gene            5410..6057
                     /locus_tag="PLT31_02050"
     CDS             5410..6057
                     /locus_tag="PLT31_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPW93948.1"
                     /translation="MSNLNELLSQVTERRQNLLQKTVSTRTRHFCMVLEDLFDPHNIS
                     AVIRTAEVFGLQDVHIIEEDNAYKVNKSILKGSFKWMSLFLYKKRMACMANLKAKGYK
                     IAVASTNTENSVLDLNLDQPTAFYLGSEFHGNHPDTLAAADYEFKLPQYGITESMNVS
                     VAAGVLMTYLDVFMQKKGREHYSLSLEEQNTLLENWLDRHVNGTENNSPIQRIDD"
     gene            6122..7030
                     /locus_tag="PLT31_02055"
     CDS             6122..7030
                     /locus_tag="PLT31_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPW93949.1"
                     /translation="MKKNTSLYFAVGLVVILAVVILVFGMFFLNDKDPRETFNTFYLR
                     FSQVSTLVLDDPVKVNGVKLGKVENIELSGHRVTVTVRLRTDVKIPKDSEVRVQNIGI
                     MGERQIGMILGDSSQYFNPGDTIVGLFDAGIAEAIGLAGEVFDSTKVLIQSVKGVLNS
                     TIAAPEFQDQFKTILTKAEDLEDRLIVIVKQTDPQLKKSLNSLNEATVKVNTLLDNVE
                     EPVNNLFVNTNGLIKNADQLINELNQVTNRLSILTQKLQAKDNTAGLLLNDRALYDDL
                     MLTVHSADSLFRIILQDGLDINVDFF"
     gene            7037..7309
                     /locus_tag="PLT31_02060"
     CDS             7037..7309
                     /locus_tag="PLT31_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HPW93950.1"
                     /translation="MIVKTLYVTNKLGVHARPAGMIVDITGQTKSDVTIIHDGQKANA
                     KSILNVMMLAIPPGSEVQFEIDGQDEEDVMMQLERLFHDNFHEESY"
     gene            7281..9134
                     /gene="ptsP"
                     /locus_tag="PLT31_02065"
     CDS             7281..9134
                     /gene="ptsP"
                     /locus_tag="PLT31_02065"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HPW93951.1"
                     /translation="MITSTKNHTKKRQRIVLVGIPASPGYAMGHCHTISNREVTVVEE
                     TLPNDRLAQEEQLFLKAVNNTIKELAQIKQITVNRLGIDEGRIFDAHLMILKDPTLVN
                     GILDQIKKENKNAQWAVHTQITQLISTFESHKSALMRDRAMDLKDLYHRLMGALDDSG
                     PVIIGDEIGNGVILVAHELNPSTLISLKKNQVIAFATDSGGRTSHVSILARSMEIPAV
                     SGLRNVSAMIQPEDNIIVDGTGGMIIINPNEEDIRKFEEKLEVFKRQQRELFTMRQLE
                     PMTRDGKYITLHANIELTSEADKVLDYGASGIGLYRSEFLFFRKGLPSFEEQLEAYQY
                     VLNALAPNSVTIRTLDAGGDKLVADISISNEANPFMGWRSIRVCLDQEDLFKEQLKAL
                     LSANTQGNLRILLPMISSMQELRRSKAHIETCRRELEAQGIFSPKVELGIMIEVPAAV
                     MLVDKLAKEVDFFSIGTNDLIQFTLAVDRTNELISSMFQPHHPAVLNLIHKTVMAAHR
                     EGIPVAVCGEMSSDPMSTLLLVGLGVDELSMTPWSIMECKKIIRSINYEDVRETAQNV
                     LKMDDTESVNAYLKKKYSQTITDLGISSFISAQDLDDGDSNSSLISSLNEN"
     gene            9124..11307
                     /locus_tag="PLT31_02070"
     CDS             9124..11307
                     /locus_tag="PLT31_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HPW93952.1"
                     /translation="MKIKIICSFLFFIACSAFASDSIISYEQLPPYRFQDRVIRYERS
                     LRAMNDSSLSEEARQFSKAAVFYYSENWDSAFQAYSQLIKKDSLLERSVLIRMARIRF
                     KQGDITEMRKVLAQGISLEKDEQWQKQSWRLRSEAVLMDSTLSENQKADSLEAFLNKY
                     PRSEEAITIRYRYAVFLDHLNKKSKAKKAYLRVLADHSRFSDSAYIAIRNIREISDDE
                     TFEEKLAYAKITCKKDYAAECVDLIDSIFNPKNPPLSKEDKTALLQSKAVALRSLKRD
                     EESIATFKLLLDSIEMKSLWIQSSLRLMRKNPTKYKKEIRSLDSILQESKRYSSENAN
                     NLWVRGFEHEQNKRFQKAIDSYQKLSHPRFRNNSKRQWAKFRIAFIYYKQGKLEEAEK
                     AFLEAKKEPFTWSSNASRMFLGDIYHQQGKDSLAKEAYLDCIQDFPLAYYAHRSRSKL
                     IEYKLMDSSQVPFARGYEASEEETLQWIRNAQKTGKPDPTYSKDRYDRMKHLFLFGFT
                     EEAFDLYNEARYKNAKRLDFLYEYGNLFYEMGEIADGYRLARQFQNVIARQKLHRAPI
                     RVLKFLYPIPYLDAVKFRSGDAIDPFFVYSVMRQESIFNFEISSPVGARGLLQIMPAT
                     GKQLAKLEEIPDFDPNMLYNPYLNIRLGTRYLIDLKKEYDNDYMYVLGNYNAGPKPTK
                     RWQAAGGNLPWDIRAEEISYWETRDYVKRVMGNYWIYQEIWNQLP"
     gene            11312..12154
                     /locus_tag="PLT31_02075"
     CDS             11312..12154
                     /locus_tag="PLT31_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPW93953.1"
                     /translation="MLFFLLTLIPAICFACGNILAKSGLSAVAHTVSIKQPWAFIKLI
                     LRNKYWWMGFSLSAIANLGYYLAMALYDISLVQPMMTLNPVLTALFGFWFLKEHLTKR
                     IVAAIGFVFFGLLLSASHAGESSSVQNITGLWLFAAFFVLISILFKLGHSSVETSDSF
                     IMGTGFGVSAAFYKSMTLSFGESMQWTSSEIIQWITDPRLFAFIVTYMTAYIYSQIAF
                     TRGRALFIIPFSAALGAAIPILAGTIVFSENLSLEKFFSIIFVLLGSGLFIIKRHRKK
                     NPTT"
     gene            complement(12147..14888)
                     /locus_tag="PLT31_02080"
     CDS             complement(12147..14888)
                     /locus_tag="PLT31_02080"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HPW93954.1"
                     /translation="MRKNYHQYILALCGLIFLFGCSSEPEDKAIIPEEKKTLTYVGHS
                     PVEITEIDSRNSFYKDTDGDDPGWVELYNPADTAVNLSGYSFVDNHEIGNIWTFGNVV
                     VAPKSYVVVFFSEKNRPNAQNPSDTINMIGSGCWGWSDIDNSPIAGTSTVEPVYTNYC
                     YNTETGRRFSAQMQHGEDGELGWHSTCVFVGALTSDPEDVVDLSKTNELLLTGYLPKN
                     MELEVRLAQTDIDDYKGWFSTIKGTGDSNSTYRILLPQNTNFPDLKNIYGTRFSPTYN
                     EMRMLSFEFTSYIAYNSGSEAHTSFKLKKNGGSLYLINQEGILDSVAYPELPPEKTWS
                     KRESVWGYADASPKGAVLSTIWTEKLGKLELPPSGFFSAPFNVEFTAPEGQEIRCEQG
                     GFVPKETSPLFAGSLTIDHSMVLRCALFKAGALTGEVVSRTYLFETQPTVATVFITGD
                     PLAFFDPDTGIYELGPNASPEMPNYGANFWEDRELPIHVDLIEVNAQTPAFSMNAGYQ
                     IFGNYSRSNPKKSVAIVFREKYGDNKLEYPLFPEHPNLTEFKVFLLRNNGSSFGLDYI
                     RDMLSSSISRGLGVDYQKGRASVVFYNGEYFGIHNIRERSNEYYFETNYGLDPNAIDL
                     LKANNEASSGSSVGYTEMMDWIQANGVVSNENYQFVSTLMDVNNYLNYVHTEIFNNNR
                     DWPGNNLKKWRNTNPITPWKWFLYDQDFGWDYPYVESQYTYNIFEFVTEENGPDWPNG
                     PEHTLLLRKLLENNSFKAAFINRYTTLLSMNFEASRLDERIQTLMSAIEAEIPRDQDR
                     WLNSWQINNMESELEKIKAFAHTRQEASLLEMQSFFSLPAVKKVTLLSEGSGSIAVHD
                     LPLDRSSVTISFFEQLPVFLSAVPSAGGIFKEWSDGELSPIREINPSEVDTLKAIFR"
     gene            complement(14940..>15337)
                     /locus_tag="PLT31_02085"
     CDS             complement(14940..>15337)
                     /locus_tag="PLT31_02085"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93955.1"
                     /translation="LEMKLPSVKRVLLDNQDIPTGNYENDTRFQERVLIKNTEINDCF
                     VLDPLTSKAVSTLHNPKNGDSLEIWQDSNYKMIQIYTPPDRQSIAIEPMTHEPDVLNH
                     HRDLLIIAPGESLSFSFGSCYSSFVQKPL"
     assembly_gap    15338..15347
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15348..15918)
                     /locus_tag="PLT31_02090"
     CDS             complement(<15348..15918)
                     /locus_tag="PLT31_02090"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HPW93956.1"
                     /translation="MSKFQLLSRPLGAIERFVIQCSSGAELEILSGHGAGLNAWRVAN
                     ASGMLNLLYGYTDEAVFRKIQADTSAGVRLSPFAGRTNNAKWQLNGKSYYLDNNVSWA
                     PHALHGLLHTKPWRFVSFKEENDFAELTLAYDYVGSHQGFPFPYRAETRFRFSGNSYQ
                     VISTVTNRGVTPMPYSEGYHPYYSLNTRID"
BASE COUNT         5045 a         3179 c         2812 g         5007 t
ORIGIN      
        1 agactgcact ggagctacta tttgcactgc tagagctcaa gatggcgcta gaagaagaag
       61 acttagcgct agaagaggaa gacatagaca atcacagaat cccctacaga caacccttcc
      121 agcacctgaa cgcgtacgcc gtcattgata cccgttttta tgcgtctagg ttcagggcgc
      181 ccgtttacgg aaacccaaac tctagctgcg tcatccgaac gaccaaaacc ttttttccct
      241 ttccctgata tacttctttg cccctgaccc cttggcattc ctttttcata agacccttct
      301 gcaggcttaa attgaagcgc ttgcaaagga atcaccattg catttggctc ttcttttgtg
      361 acaatcgtac aagtcgctgt catccctggc aataatttga attctggatt ttctgcagaa
      421 ataacaaccg tataagtcac cacatttgaa gtcaccgttg gattcaaacg tagctcttcg
      481 acttttccaa aaaaacgatc ctttggaaac gcatctacag taaattcaac tcgctgccct
      541 tgtaatatag aacctatgtc ggcttcatct acactagcca tcactctcat ttgagataag
      601 tcacgcgcta aaatgaaaag aacaggagca ctcattgaag ccgctaccgt ttgccccaca
      661 tctacagagc gttctaaaac aactccatct atcgggcttt ttataacaca ataactcaaa
      721 tttaatttgg cctgttgtac gtcactttta ctccgttcca tcgaaaagcg agcggcattc
      781 atattatatt cggctgtttc taattctaca gcacttacac tttttgattc cgcgagtttc
      841 tgcgttcttt caaaaatatt tttcttgtat tgataatcat tttctgctgc tttataagca
      901 agttccgact gacgcaagtt agtaatccat tttgatttat ctaattctgc aagcacttgc
      961 ccttttttca ctttactatt aaaatcaaca taaatacggc ttaaatcgcc agatacttga
     1021 gtgcctattt ctacacgatc cacaggctcc aatgaacctg tcgcagatat gcttgttaat
     1081 aaaagagtat cttgttccac agccgcactt acaaaaacgc ctaccgaaga ctcttttgta
     1141 ttaaagaaaa taaagcgagc accaagaatt agtgtagcga gaataacaag aattatgctg
     1201 actttaatga tttttctcat tttttgcctt tttagggatc ctttataaaa tcctatttta
     1261 caatttagat gttttagccc tgctaaacgt tccataaaaa ctgtaaaaca cccaaataat
     1321 tacttttatt cttttttata ccatttttat aagtctcttc tttcgtacga taaaatcaag
     1381 aaccaatctc ggtttgaaaa atgttttttt cacgaagagg ctcgtattta tgtaaatcaa
     1441 attggtgtat ccccgacttt atgactaatt tctttcggtt tattattcta ttttaagatt
     1501 tgtatgaaaa aagctctttt attattaatc ccttcttttc tatttgcatt gcaactgcct
     1561 gtacataaag aagttctaga caatggtcta actgttttgt tacaccccaa taaacaatcc
     1621 cctactgttt cttgtcgtct tttttatgtc acaggttctg ttcatgaagt gccaggatct
     1681 tctggtttgg cacatatgct tgaacatgaa ttgtttaaag ggactcgtaa agtgggcatt
     1741 actgatagta ttgctgattc tgttttcatg tataagcaag attccctaca agcccttatg
     1801 cgcccactca ttcaaaaagg ggatacaact cactatctaa aactaaaagc ggaacatgat
     1861 tccattgtga atgagcatcg aaaaattttt gtcaaggatg aactttggag tacttatcaa
     1921 gcggcaggtg gaactggttt aaatgctttt actagtgatt taatgaccgc ttatatcgtc
     1981 acattaccta aaaataaagt ggaactcttt ttgtggctcg aagccgatcg tatgcaaaat
     2041 gcgattcttc gagaatttta ttctgaacga gatgtcattc gtgaagaacg ccgcatgcgt
     2101 tacgatgata ggcctactgg acgtttttat gagactttaa attctatgat ttacgaagcc
     2161 ttcccttatc gaattcctac tataggctgg ccttccgaca ttgaaaatct gactcgtgaa
     2221 aaagccgctg aacattatca taaatactac aaaccaagaa acgctatttt agttcttgct
     2281 ggcgatcttg atacagccga agtaatgcct ttaattaaaa aatattttgg ctccattcct
     2341 ccaggggaac catttcctgc aattacggta cgtgaccctg agcaagctgg agaaaagcgt
     2401 ttaatcgtac ggcgccaaga tgctcctcat ttactcacca ttgtatttaa aacaccagaa
     2461 gtcggagact ctaaactcta tgctttagac attgcagaag gggtcttaaa tggtcgttct
     2521 ggtcgattac ataaaagact cgttcaagaa gaaaatcttg ctgtgaaagt aagtgcaagt
     2581 aatagcccaa acaaatacgt ttctgaattt tcaattcaag cagaactgga acccgatgca
     2641 gaccctgcaa aagcagaagc gattatttgg gaagagcttg aaaaactaaa agtagaactc
     2701 ttaagtcaac gtgattttca aaaagtaaaa aaccgtgctt atgcttcttt agtcagaagt
     2761 ttaaccgaca tggaaaatgt cgccaccatg ctcgcttggt atgaaatgta tggtgattat
     2821 catatctttt tgaattgggc aaatgcttta gaaaaagtca ctgctcaaga agtacaaaaa
     2881 gcggcgcaag aaacgttctc aaggaataag gccaccattg gaattttaac taaagagaat
     2941 gcaaaatgaa atggatttcc cctttaaaca agttcccttc taaaaagtct tctactacac
     3001 cttcaaaagc catctttaag gaatccttaa tgccttttaa aatgactctt ttctttttga
     3061 ttctcttgat ttttggctgc tcttccactc cagttaaaaa caacgtttct gaggtgactc
     3121 ctgtttctaa aacaaacgaa gaacaagtgg tttctaaatc tacaaacaaa gccttgcctt
     3181 cttcttataa agagatctct ttcccagaat ttacttatgt tgctccacat ccaaaagaat
     3241 atcgagtcca actagatgat agcatcacag gttatgttgt tgtagaccgc tctcttcctt
     3301 taatttcttt ctctatgttt tttaaggaag ccacaattcc ttctaacatt aaagaaacgg
     3361 cagcacttga acttctttct ccgatgtttc gtcgcggagg ttcaagtgat atttcagcaa
     3421 atgctttaga tgactcctta gagtttatta gcgcctccat tcaaggggga ctcggctctt
     3481 actacagcaa aatttctatc gattgcttat ctaaaaattt tgaagaaatg ctttctttat
     3541 caaaatctgt ttatacaaaa ccaggctttg atgaaaagca acttgaaatt caaaaaacgt
     3601 cttatatcac cgcatataaa cgtcgttttg acaaccccgc ttcgattcta tcggctcttc
     3661 gttccaaagt caattatgct tcttcaccta gattatggaa ccctaccata gaagagtatc
     3721 aaaatgtttc caaaaaagat ctcgaacgtt ttgctaaaac aaagttctcc gcaaaccgca
     3781 tcgtctttgc tttggctggt gatattgaaa aagactctgc tcttgtttta ttaaaaaaat
     3841 attttgctga ctggaaaaaa acgacttcaa gagaaccaac tccttccatg gctctcttaa
     3901 aaaaacctgg cgtttatgca gtagaaaaag atattactca agcgaacatt tctatcaatc
     3961 aaccttttgt taaaaggcct catcctgatt attatcctac tgctgtagca agttttatct
     4021 taggtggagg cagcttttct tcacgcttaa cttcaaaaat cagatccgaa gaaggccttg
     4081 cctatagtgt ttatagccat gctggtaatg attataatga caccgcctta acaaccattg
     4141 ctctacaaac aaaagcggaa agtgcttttt ttgctgttca attaatcatt caagaaattc
     4201 aaaaactaag taaagaaggg cctactcttg aagagcttga gcttgcgaaa aagactttga
     4261 ttgaaagtct tcatggttta tttgattccc cttctagcac tgcttctatt tttgctgtcg
     4321 atgaattagt tggtaaatca caagaccatt atttagagta tgttcaagag atcaacgcca
     4381 tcactcctga gcaagtcaaa gcgatgacag caaagtattt tgatcctcag aaaatgacca
     4441 tctccattgt tgggcctaaa aagtcttggg ataaatttga aaatgtgact ataattccta
     4501 tccaagacct ttactttagg taactataaa tacaaatgcg attcttactc tttctgttca
     4561 ttcttcttag cactactcca ttgatcatgg cctatgatca aaagagcatg tgttcgagtg
     4621 caaaacaatg ccttaaatta gatattgcag ggtgctcagc tgaagacaaa aaaccgaact
     4681 ctaagattaa atacaatgat gatttttgtg caccttatat tgagctcaaa aaacgtggcg
     4741 tctcattaga aacagaaatc agtaaagaaa tttttggtcg cttaggtagt caatacaggg
     4801 ccatttatat taacgaaggt gttcttcaaa ccactcctgg aatgatgagt tacttatttg
     4861 accacatgtc ttttactgct aaactgatta atgcatatca aggtggcgct tattctatcg
     4921 attataaaac gcctaatcgg cgtgtctttt ctggaacgaa tggtagaagt ttatcgggcg
     4981 attttatttg ggtggctcaa gatagtgcag gccaaaaact gggttttcga aatgtatttt
     5041 ttggcgtagg gcgagtgaaa attttaagat ggcatctgac tggaacagct atcgcctttt
     5101 tagacatgga ttctatcggt aaaaatcaag ttcgctataa gttaacagcc attgtttttc
     5161 cagggaatgc ggttctaaat tccattatgc acatggacgc ttttaagaat atcgtcgcag
     5221 aaaaaatcaa tttgattata aaagatattc aagaatcctc gtcacgctat gccaaaggcg
     5281 ataaaaaacc gattggacaa agtaaagcac ttcaaactaa aaatgaatta atccatttaa
     5341 aagaatttga atcagtcgtt caaggaaccc cttggactct tggcgactat tttaagaaga
     5401 aaggatctta tgtcaaacct aaatgaatta ttatctcaag ttacagaacg ccgtcaaaat
     5461 ctgcttcaaa aaacagtaag cacaagaacg cgtcattttt gcatggtttt agaagatctt
     5521 tttgatccac ataatatctc ggcagtgatt cgcacggcag aagtctttgg cctacaagac
     5581 gttcatatca ttgaagaaga taacgcctat aaagtgaata aatccattct aaaaggatct
     5641 ttcaaatgga tgtctctttt tttatacaaa aaaagaatgg cttgtatggc taatctaaaa
     5701 gcgaaaggct ataaaattgc tgtggcaagt accaatacag aaaactccgt attagatctc
     5761 aatttagacc aacctaccgc tttttatctc ggaagcgaat ttcatggtaa tcatccagac
     5821 actttagcgg cagcagacta cgaattcaaa ttaccccagt atggcattac agaatccatg
     5881 aatgtttctg tcgctgcagg cgttttaatg acttatttag acgtttttat gcaaaaaaaa
     5941 ggaagggagc attattctct ctctttagaa gaacaaaaca ctttactcga aaattggctt
     6001 gatcgacatg ttaatggaac tgaaaacaat tcccctatac aacgaattga cgattaaaag
     6061 cgcctttttt cagataaaat tcatatgatc gtatcatgaa taaactaaat tatcagcaat
     6121 aatgaagaag aatactagtt tatactttgc cgttgggctt gtcgttatat tagccgtcgt
     6181 cattcttgtt ttcggtatgt ttttcttaaa cgataaagac cctcgtgaaa cctttaatac
     6241 tttttatctg cgattttctc aagtgagtac actcgttcta gatgatcctg ttaaagtgaa
     6301 tggcgttaaa ctagggaaag tggaaaatat cgaactaagc ggtcaccgcg tcactgtcac
     6361 tgttcgactt cgtaccgacg ttaaaattcc taaagattca gaagtgcgag ttcaaaacat
     6421 cggcatcatg ggtgaacgtc aaattggaat gattcttggc gattcgagcc aatattttaa
     6481 cccaggtgat accattgtcg gtctttttga tgctggcatt gctgaagcga ttggtctagc
     6541 aggggaagtc tttgatagta ccaaagtatt aattcagtcc gtgaagggcg tattaaattc
     6601 gactattgcc gctcctgaat ttcaggatca atttaagaca atcctaacga aagcggaaga
     6661 tttggaagat cgcttgattg tgattgtaaa gcaaactgat cctcaactca aaaagagctt
     6721 aaatagcctt aatgaagcaa ccgtaaaagt aaacactctg cttgataatg tagaagagcc
     6781 tgtaaacaat ctttttgtaa acactaatgg cctaatcaaa aatgccgatc agttgatcaa
     6841 tgagttgaat caagttacaa atcgtttgag tattctcact caaaaacttc aagcgaaaga
     6901 caatacagca ggcttattat taaatgatcg cgctttatat gatgatttaa tgttaacagt
     6961 acattccgca gatagtttat tccgtattat attacaagat ggcttagata ttaatgtgga
     7021 tttcttttga ggagttatga ttgttaaaac gctttatgta acgaataaac ttggcgtcca
     7081 cgcgagaccc gctggaatga ttgtcgatat tacgggacaa acaaaaagcg atgtgactat
     7141 tattcacgat ggtcaaaagg ccaatgcgaa gagcatttta aatgtaatga tgttagcgat
     7201 tcctccagga tccgaagttc aatttgaaat tgacggccaa gatgaggaag atgtaatgat
     7261 gcaactcgaa aggctttttc atgataactt ccacgaagaa tcatactaaa aaaagacaac
     7321 gtattgttct tgtggggatc ccagcttctc ctggttatgc gatggggcat tgccatacta
     7381 ttagcaatcg ggaagtaact gtcgtcgaag aaactcttcc aaatgatcgg ctcgctcaag
     7441 aagaacaact tttcttaaaa gcggttaata ataccattaa agaattagcc cagatcaagc
     7501 aaatcacagt caatcgactg ggtatagacg aagggcgtat ttttgacgcc cacttaatga
     7561 tcttaaaaga tccaacctta gtaaatggta ttttagatca aatcaaaaaa gaaaataaaa
     7621 atgcgcaatg ggcggttcat acacaaatca ctcaattgat ttcaacgttt gaatcccata
     7681 aatccgcttt aatgcgtgat cgcgcgatgg atctaaaaga cctttaccat cgcttaatgg
     7741 gtgctcttga tgactctggc ccagttatta taggagatga aatcggaaat ggtgttattc
     7801 ttgtcgctca tgaattaaat cctagtactt taatttcttt aaagaagaac caagtcatcg
     7861 cttttgcgac agactctggt ggccggacta gtcatgtctc catcttagct cgctcaatgg
     7921 aaattcctgc tgtttctggt cttcgaaatg tttctgcaat gatccaacca gaagacaata
     7981 ttattgtcga tggcactggt gggatgataa tcattaatcc caacgaagaa gacattcgaa
     8041 agttcgaaga aaaactcgaa gtatttaaga ggcagcagcg agaactgttt acgatgcgac
     8101 agcttgagcc aatgactcga gatggaaaat acatcactct acatgcaaat atcgaactta
     8161 cttctgaagc agataaagtt ttggattatg gagcgagtgg aattggttta taccgttctg
     8221 aatttttgtt ttttagaaaa gggctcccct ctttcgaaga acaattagaa gcgtatcaat
     8281 atgttttaaa tgctcttgct cctaatagtg tcactatccg tactttagat gctggtggtg
     8341 ataaacttgt tgctgatata tctatatcca atgaagcgaa tccctttatg ggctggcgtt
     8401 ctattcgagt ttgcttagac caagaagatc tctttaaaga acagcttaaa gccttactct
     8461 ctgcaaatac tcaagggaat ttaagaatac tacttccaat gatttcttct atgcaagagc
     8521 taagacgttc aaaagctcat atagaaacat gcagaagaga attagaagct caaggtattt
     8581 tttctcctaa agtagaactt ggcatcatga tagaagtgcc tgcggctgta atgcttgtag
     8641 acaagctcgc aaaagaagtc gattttttca gtattggaac aaatgacttg atccaattta
     8701 ccttagctgt agatcgcact aatgaattaa tctcttcgat gttccaacct catcatcccg
     8761 ccgtattaaa tttgattcat aaaacagtga tggccgcaca cagagaagga atacccgttg
     8821 ccgtttgcgg agaaatgagt tcggatccaa tgagtaccct tcttttagtg gggcttggtg
     8881 tagatgaatt atctatgact ccttggagta tcatggaatg caaaaagatt attcgttcta
     8941 ttaactatga agatgttcgt gaaacagcac aaaatgtttt gaaaatggat gacaccgaaa
     9001 gcgttaatgc ttatcttaaa aagaagtatt cacaaaccat tacggattta ggtatctcca
     9061 gctttatttc tgctcaagat ttagatgatg gagattcaaa ttcatcatta attagtagtc
     9121 tcaatgaaaa ttaaaattat ttgttctttt ttatttttta tcgcttgttc agcctttgct
     9181 tccgattcta ttataagcta cgaacagctt cctccttacc gatttcaaga tcgtgtgata
     9241 cgttatgaaa ggtctcttcg agccatgaac gattcctctt taagcgaaga agctcgtcaa
     9301 ttttctaaag cagccgtctt ttattactcc gaaaattggg acagtgcatt tcaagcctac
     9361 agtcaattaa ttaagaaaga ttcactttta gagagaagcg tgcttattcg tatggcacgt
     9421 attcgattta agcaaggtga tattactgaa atgcgaaaag tcctcgctca aggaatttct
     9481 ctagaaaaag acgaacaatg gcaaaaacaa tcttggcgtt tacgtagtga agctgtgttg
     9541 atggattcaa ctctttctga aaatcaaaag gcagactcgc tagaagcctt cttaaataaa
     9601 tacccaagaa gtgaagaagc tattaccatt cgttatcgct atgctgtctt cttagatcat
     9661 cttaacaaaa aatcaaaagc taaaaaagcc tatttacgag ttcttgcaga tcattctcga
     9721 ttcagcgatt cagcctatat tgccattcga aatattcgcg aaatctctga tgatgaaacg
     9781 tttgaagaaa aactcgccta tgcaaaaatt acatgtaaaa aagattatgc cgctgagtgc
     9841 gtggatttaa tagactctat ttttaatcct aaaaaccctc ccttaagtaa agaagataaa
     9901 acagctcttt tgcaaagtaa agcagtcgct ctccgttcac tgaagcgcga cgaagaatct
     9961 attgccacat tcaagttact tcttgactct attgaaatga agtctctttg gattcaatcg
    10021 agtcttcgct taatgagaaa aaatcctact aaatacaaaa aagaaattag atctctagat
    10081 tctattcttc aagagagcaa acgttatagt agcgaaaatg caaataatct ttgggttaga
    10141 ggttttgaac atgaacaaaa caaacgcttt caaaaagcca ttgattctta tcaaaaatta
    10201 tctcacccgc gttttagaaa caactctaaa aggcaatggg caaagtttag aatagcgttt
    10261 atatactaca aacaaggaaa gctcgaagaa gcagagaaag catttttaga agctaaaaaa
    10321 gaaccattca catggagcag taatgcgagt cgaatgttct tgggagatat ataccaccaa
    10381 caaggcaaag attctctcgc taaagaagcc tatttagatt gcattcaaga ttttcctctc
    10441 gcttattatg cgcaccgtag ccgcagtaaa ctaattgaat acaaattaat ggattcttct
    10501 caagttcctt ttgcaagagg ctatgaagct tctgaagaag aaacactcca gtggattcga
    10561 aacgctcaaa aaacaggaaa acctgatcca acgtattcta aagatcgcta tgatcgaatg
    10621 aagcacctat tcctttttgg ttttaccgaa gaagcatttg atttatataa tgaagctcgt
    10681 tataaaaatg ccaaacgtct cgacttcttg tatgaatacg gaaatctttt ttatgaaatg
    10741 ggcgaaatcg ctgatggcta tcgacttgct cgtcaattcc aaaatgtgat cgctcgccaa
    10801 aaacttcatc gcgctcccat tcgagtctta aaatttctct accctattcc ttatctggat
    10861 gctgttaaat tcagatctgg cgatgccatc gacccattct ttgtttatag cgtaatgcgt
    10921 caagaatcga tttttaattt tgaaatatct tctcctgttg gcgctcgcgg tttattacag
    10981 attatgccag ccacaggaaa acagcttgct aaattagaag aaattcctga ttttgatcct
    11041 aacatgcttt acaatcccta tttaaatatt cgattaggta cacgctattt aatagatctc
    11101 aaaaaagaat acgataatga ttacatgtat gttttaggca attataatgc tggtccaaaa
    11161 ccaacgaaac gctggcaagc cgcagggggt aatcttcctt gggatatccg tgctgaagaa
    11221 atcagctatt gggaaactag agattacgta aaacgcgtca tgggtaatta ttggatttat
    11281 caagagatct ggaatcaact cccctaattc tatgttgttt tttttactga ctttaattcc
    11341 tgctatctgt tttgcttgtg gaaacattct tgcgaaatca ggtctttctg ctgtagctca
    11401 tactgtaagt attaaacaac cctgggcttt tataaaatta attcttagga ataaatactg
    11461 gtggatgggt ttttcattat ctgcgattgc aaatcttgga tattacctag ccatggctct
    11521 ttatgatatc agccttgtcc aaccgatgat gaccttaaat cctgttctta cggctttatt
    11581 tggtttctgg tttttaaaag agcatttaac aaaacgcatt gtagctgcga ttgggtttgt
    11641 tttttttgga ttattacttt cagcgtctca tgcaggggaa agttccagcg ttcaaaatat
    11701 tactggctta tggcttttcg ctgctttctt tgttttgatt tccattcttt tcaagttagg
    11761 ccattcctct gttgaaacat ccgattcctt tattatggga acggggtttg gcgtttctgc
    11821 ggcattttat aaaagtatga cccttagttt tggagaatcc atgcagtgga cttcttcaga
    11881 aataattcaa tggattacag acccacgcct ctttgccttt attgttactt atatgacagc
    11941 gtacatttat tctcaaattg cattcaccag agggcgagct ctttttatta ttccattcag
    12001 cgctgctcta ggagctgcga ttccaattct tgcaggcacc attgtttttt cagaaaatct
    12061 ttctctagag aagtttttct caatcatctt tgtactctta ggatcaggcc tttttattat
    12121 taagcgacat agaaagaaaa accctactac ctaaaaatag ctttcaatgt atctacttct
    12181 gaaggattta tttctcgaat tggagaaagc tcaccatcac tccactcttt aaatatgcct
    12241 ccagcactag gcactgcact taaaaatact ggcaactgtt caaaaaatga tatcgtcaca
    12301 gaagaacggt ctaatggtaa atcatgcaca gcaatagacc cagaaccttc gcttaacaaa
    12361 gtcacctttt ttaccgcagg taagctaaag aagctctgca tttcaagtaa cgatgcctct
    12421 tgtcttgtat gagcaaaagc ttttattttt tcaagctctg attccatatt attgatttgc
    12481 caagaattta accaacgatc ttgatctctt gggatttcag cttcaatcgc tgacattaat
    12541 gtttgaatcc gttcatctaa acgacttgct tcaaaattca tcgacaacaa cgtggtgtat
    12601 cgattaataa acgccgcttt aaaagaatta ttttctaaaa gcttacgtaa taaaagtgtg
    12661 tgctctggcc catttggcca atctgggcca ttctcttcag tgacaaattc aaaaatatta
    12721 taagtatact gcgattctac ataagggtaa tcccaaccaa aatcctgatc atataaaaac
    12781 catttccaag gagtgatagg gtttgtgttg cgccatttct ttaggttatt cccaggccag
    12841 tcacgattgt tattaaagat ttcggtatgt acataattca aataattatt cacatccatt
    12901 aatgttgaga caaattgata attctcattt gagaccactc catttgcctg aatccaatcc
    12961 atcatttcgg tataacccac agacgatcca gagctcgctt cgttatttgc ttttaataaa
    13021 tcaatcgcat ttggatctaa accataatta gtttcaaaat aatactcatt agaacgttct
    13081 cgaatattat ggataccaaa atactctcca ttataaaaaa cgactgaagc acgccctttt
    13141 tgataatcaa ctcctaaacc cctgcttatt gaacttgaaa gcatatctcg aatgtaatca
    13201 aggccaaaac tactaccatt gtttcttaat aagaacacct taaactccgt tagattcggg
    13261 tgctcaggga acaaagggta ttctagcttg ttgtcgccat atttttctct aaatacaatg
    13321 gccactgatt ttttggggtt cgaacgacta taatttccaa aaatctgata tcctgcattc
    13381 atagaaaaag caggagtctg agcattcact tcaatcaaat ccacgtgaat tggaagctct
    13441 cgatcttccc agaaattagc cccataattt ggcatttcag gagaagcatt aggccctaat
    13501 tcataaattc cagtatctgg atcgaaaaag gctaacggat ctccagtaat aaaaacagta
    13561 gccacagtcg gttgagtttc aaaaagataa gttctcgaaa cgacttctcc agttaaagca
    13621 cctgctttaa ataaagcaca acgcaaaacc atagaatgat caatcgtcaa agagccagca
    13681 aaaagcgggc ttgtttcctt tggaacaaag ccaccttgtt cacaacgaat ctcctgacct
    13741 tcaggagctg taaactctac attaaaagga gcgctaaaaa agcctgatgg gggtaattct
    13801 agttttccta atttttcagt ccatatcgtt gatagcacag cgcccttagg acttgcatct
    13861 gcataacccc agacactttc cctttttgac catgtttttt ctggaggtaa ttctgggtaa
    13921 gctactgaat ccaaaatgcc ttcttgatta attaaatata ggctaccacc gttcttcttt
    13981 aacttaaatg atgtatgggc ttcacttcca ctattgtaag caatataaga agtaaattca
    14041 aatgaaagca ttctcatttc attgtatgtt ggagaaaagc gagttccata aatatttttc
    14101 aaatcaggga aattagtatt ctgtggcaac aaaattcgat aggtagaatt agaatcgccc
    14161 gtgcctttaa ttgtcgaaaa ccatccttta taatcatcaa tatctgtttg agcaagacgt
    14221 acttctaatt ccatattttt aggcaaataa ccagttaaaa gcaattcgtt tgttttactc
    14281 aaatcaacaa catcttctgg atcactcgtt aacgctccaa caaatacaca tgtcgaatgc
    14341 caacccaatt ctccatcttc tccatgttgc atttgagctg aaaagcgacg ccccgtttct
    14401 gtattataac aatagtttgt atacacaggt tcaacagtac ttgttcctgc aattggtgaa
    14461 ttgtctatat ccgaccagcc ccaacatcca ctcccaatca tattaatcgt atcagaagga
    14521 ttctgtgcat taggtctatt tttttctgaa aagaatacaa ccacgtaact ttttggagcc
    14581 acgacaacat ttccaaaagt ccatatattc cctatttcat gattatccac aaaagagtaa
    14641 cctgacaaat tgaccgcagt atctgcagga ttatataatt ctacccagcc tggatcatct
    14701 ccatctgtat ctttatagaa actatttctg ctgtcaattt cagtaatctc aactggagaa
    14761 tgacctacat aggttaatgt ttttttctct tctggaataa tagccttatc ttcaggctct
    14821 gaagaacacc caaacaagaa aattaaacca caaagggcca aaatatattg atgataattt
    14881 tttcgcattt tataaaaata cgtttcttct aatcacctag atgttatttt ttacatctct
    14941 tataacggtt tttgcacaaa agaagaataa caagaaccaa agctaaaaga caaagactcc
    15001 ccaggtgcta taataagtaa atcccgatgg tgatttaaaa catctggttc atgagtcatt
    15061 ggctcaatcg ctatactttg cctatcagga ggtgtataga tctgaatcat tttataattc
    15121 gaatcctgcc aaatttctaa agaatcgcca tttttagggt tgtgaagagt cgaaacggct
    15181 tttgatgtta atggatctaa tacaaaacaa tcattaattt ctgtattttt tattaacact
    15241 ctttcttgga atcgcgtatc attttcataa ttcccagttg gaatatcttg attatctaaa
    15301 agcacccttt ttactgaagg taacttcatc tctaactnnn nnnnnnncat caattcgagt
    15361 atttagagaa taataagggt gataaccttc agaatatggc attggagtta caccacgatt
    15421 tgtgactgta gaaatcactt gatatgaatt tccagaaaat cgaaaccgcg tttctgccct
    15481 atatggaaat ggaaaccctt gatgactacc tacataatca taagcaagtg ttagttctgc
    15541 aaaatcattt tcctctttaa aagaaacaaa acgccaaggc ttggtatgta ataatccatg
    15601 taaggcatgc ggagcccaag aaacattatt atcaagataa taggactttc cattgagttg
    15661 ccatttagca ttattggttc tacctgcaaa aggagaaagt cgaacgcctg cacttgtatc
    15721 cgcttggatt tttctaaaaa cagcctcgtc cgtataacca tacaagagat tgagcatacc
    15781 tgaagcatta gctactcgcc aagcatttaa acctgcacca tgcccagaaa gaatttcaag
    15841 ttctgctcca gaagagcact gaatcacaaa acgttctatt gcccctaaag ggcgtgataa
    15901 taattggaat tttgacatgc actaaaaata ggattttttt tgtttttttc atgattaatc
    15961 tcattaattc ttaaaactac tgttattgat tgaaatggtt ttttcattaa aactcaataa
    16021 tactatggtt gacgggcatg gctattacct cct
//
LOCUS       DAOOBB010000023        15836 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_4828_length_15836_cov_5.347697, whole genome shotgun sequence.
ACCESSION   DAOOBB010000023 DAOOBB010000000
VERSION     DAOOBB010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 15836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15836
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4828_length_15836_cov_5.347697"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            324..3557
                     /locus_tag="PLT31_02095"
     CDS             324..3557
                     /locus_tag="PLT31_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93957.1"
                     /translation="MVTILLTSTLEALPIYGNQSHLRWKTVSSEHFIFNYPSEYTEHA
                     GIVAAYAEAVYDSIVKRYRTDLHKKVNVNLHDALYANGNAIPTENTINLWLTNWDFKL
                     RSSHHWLSDVITHEFSHLVSIENGSKLPPFVYGFQVSYSDYYNENTTSNFLSMFPFTL
                     QPLWLAEGTAQFESARMGFDGWDSHRDMLLRTAALEEDLLSLEFMHSFANNALKAELG
                     PYTQGFSLIRYIASHYGEETISKIWSEMSRIHRVTVDAAIQSVLGISEKELYEAWKRE
                     ITEQYEKQKESLGLLVMGEKWTTDAFYQDFLVVAGNSLYGISNFGGDWFDGSLFKMPK
                     TRDSLTDEQSKKDSLVILDFEGTIDIKDYSKSGFKPKKAWFERGFSIRESKDKGPLLA
                     YVHYKNKDRNGRTYFDLDLLDTNGKVEHITSFADAVYPDISPNVNEIVFARRLPNSSR
                     FALSLAPISKEDDSYDEEYVDIFIPEDSLFYYNIYSPKFSPDGKKITFSFFDNTTRGI
                     AVIDRDGKNLKLFSSKNIDSRDPNWIDSERIIYSHDGNGIFNLYELNLATGKTTPLTN
                     VLGGAFTPVVDSSTIFYTGYDKDGFSIYSLKLDEPTQDTTIQKKPLELKGIKPQKNNK
                     EIELLDITLHGVETNYKPIPTIPLLVPLISFEERAPDFTVAGDGEVVPKIGLIMLLSD
                     PLKKNLLQVGGLIEISKDFDYFNSSGLNPSYGRDFFALLENRSFPITLQLNYMHSNYV
                     NKDTVRYEDPRSYEDSISTSHYAVPVHSFMGGLGYSLFKKNDSLTISLGYDWADFNLY
                     EDDLRWTYHKRVNAGVSLSHYSGFSEDNSNAISGEGNGISLSYQYSNADLYRPGTFAE
                     SFTVSASGAIKPIYRNFQIHELGLSLFGSLDNPIHSGARFAAGANVSGILSWKSKNKS
                     DTLDAFYYHPLFLEGYPYLISTEDFARSGTKTALAQLHYIFPIYKDFRKHLWIFTSRD
                     FFIDVFAQVGTAWNSSWFDKEALKNIDLWDRSVGFEIRLANTLFYSIPLDISLSFARG
                     LDRIAKKADGSGGSRLHRIDIPLLPTAIEPTKISFTIGMGFNNRWMK"
     gene            3559..4908
                     /gene="hemG"
                     /locus_tag="PLT31_02100"
     CDS             3559..4908
                     /gene="hemG"
                     /locus_tag="PLT31_02100"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HPW93958.1"
                     /translation="MKKIAIIGAGLTGLTTAFYLRKNNIDITLFESTQHIGGVIQTKE
                     KDQFIWETGPSTGVLGKPEAMELFEDLEALDILETAPSLAGKRYIWKGKGLHPLPSGP
                     ISGLLTPLFSWKDKFGLLFEPFRPKAENPDENLSSFVRRRLGESVLQYAVDPFVSGVY
                     AGSPDTIIPRYALPKLYNLEATYGSFLRGTYHKMKEPKTPRDLKATKKTFSAKGGLSR
                     LIERLVAVIGEERIKTKCKDLVVMNEEKGFSVSSSAFEAEHFDEVIFTGSAKGILKTF
                     PFLQNKGLEAISQIRYTAVAEVAVGFEKWEGFPLDGFGALIPSKEKRRILGILFMSSL
                     FQNRAPKGGAMLSVFVGGERHPEYVSLSDDELKALVKEELIPILKIPNFNPAIFEISR
                     HQEAIPQYDLLTPDRINAFKAAEENFPGLHLGGNGIGGIGMADRIKQGKELFLSIIS"
     gene            4970..5052
                     /locus_tag="PLT31_02105"
     tRNA            4970..5052
                     /locus_tag="PLT31_02105"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5003..5005,aa:Leu,seq:taa)
     gene            5071..5508
                     /locus_tag="PLT31_02110"
     CDS             5071..5508
                     /locus_tag="PLT31_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93959.1"
                     /translation="MIEFYPQSIYYPREAVEAKFASGELQATEKRLLEFAERHRSAIW
                     ESALEDSSEPTDAILLDNLRAFLLSIGSIHPAFEMGELIKEIKKEVWYQNETQDVSPN
                     EIAENWKKTYAIKWREARMFEAFILIERCSKEILNILRATGKV"
     gene            complement(5444..5974)
                     /locus_tag="PLT31_02115"
     CDS             complement(5444..5974)
                     /locus_tag="PLT31_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93960.1"
                     /translation="MIRRNVSVIFFLGLFLGLLAILFWKSSPQIAPLVKETPEPAPSE
                     VLFHGRLNLLTISDLYVFENTAERDIESLLLYLQGRLSSLHYLYENIYSGRKRLKEDL
                     LIGLWVEIDQDGFFKNVKVQFNNAEKNALNKPLVHFVEKYWRYKKSNKGSTSLLIPFQ
                     WHAKYSRSLLNNVQLE"
     gene            complement(5974..7668)
                     /gene="recJ"
                     /locus_tag="PLT31_02120"
     CDS             complement(5974..7668)
                     /gene="recJ"
                     /locus_tag="PLT31_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HPW93961.1"
                     /translation="MDLGNMQELLASTITSELKVPHVIARLLVKRGCTSVAEAYYMLF
                     GEKNGIQDPFLMLDMNEAVDWVLAVRDRQEKIFIFGDYDLDGLSSVALLTRGLKEIGI
                     ATEWRLPNRFSSGYGLSVSAIDEMIEAGAKNIITVDTGITAVTEIEYAKSKGLQVMVV
                     DHHQPSGDGLPACDVLLDPHREEDSYKNEELCGVGLSYKLICALFSRLGLSSAEKFLD
                     LVALGTLADLVPLTEENKMLTRSGLKKMCHSDWYGVQELCRDVLNMHSFIGGQDVLFR
                     IAPILNAPGRMGCADAALHLLLCDDPMRAPSLLASLKEWNVLRKKKEAEISEAAIRWV
                     HSRYGNDIPSVLIIDGNDWHLGVIGIVAAKVAQEFSRPTAILSLQPDGTAHASARSVL
                     GFNWHKALFEFRHLFDRWGGHANAAGFSIPVSEIESFREQLILFAANQNFFPDECEQK
                     TSFDILVALSELDEAVMDWILKMEPYGGSFPYPVFKAEKVKIHKLRELRGNHLQMEIN
                     QNGSRRFSAIAFGMGNQKSKIEQNNFEATVVFEPTWNVYNRKRSIQLAIKAMEFER"
     gene            complement(7655..8764)
                     /locus_tag="PLT31_02125"
     CDS             complement(7655..8764)
                     /locus_tag="PLT31_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPW93962.1"
                     /translation="MIEYELKGPLSQQRTRIRLGASLKENHFPQAVIIEGLPGIGKKA
                     LAMELSKALCCTNASQRPCGECFGCKMSVNSGAVENWVIPLESKEAKAKKASDVSEGS
                     SAKTVEEFTQVYIQKIIENPYSIDYLSAAAEISVDLIRSLTGRFSLKNDRVRCVIIAE
                     ADRMNESASNALLKTLEEVPPNTYFILTTSSKEQLLQTIRSRCLSIHLPPLSSEEVRD
                     LASQYVDIPISDDVLGLSQGSVGRAIYYASKATELQDLALEFLESSISGNYSDLFFNL
                     DSEELKDPAVAILFLEVLSFLITDIVRDMSGAPLRLAETTRQFKDKENTPLDLNAYTV
                     ALQSVQETMYRIGTRRTSALVCLQSLAIKLFDGFR"
     gene            complement(8761..10002)
                     /locus_tag="PLT31_02130"
     CDS             complement(8761..10002)
                     /locus_tag="PLT31_02130"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPW93963.1"
                     /translation="MRILCFLLCALVSIGSAAPLKTDTQIRNQQKALKQLEKDLVKKR
                     EQLMLLETEEKSVLNTLSLLDQNLSQTREYVSMLASNESLVAAAVKRLSLELDSLDQK
                     ISKQQDAMKKRIRTLYIQGRHSEGEELYRLLRQKENPERQLYLVRRLLSEDKMLVENL
                     SASIKERTEKKAMEAEHLKNLQVLRSKKASEEKGFVIQIERQSEMLFMLKRDKNLQKK
                     VLEEFERNQKTMILLIKKLEQKRKKEEAEAKKRLAEEKKKKSKKVQKEKPKVKKETPA
                     VVLDKAIVGPKTMPMHGPIISNYGMQEHPVLHIMTRNLGVEIKGQRGGKVKAAAKGKV
                     VMTAEIDGRGPSVIIEHEGGFYSVYGHLNSIRVQEGDAVRNGEEIGEVGDLASLNGIK
                     LYFQVSQGTQTIDPLQWLNEK"
     gene            complement(10009..10815)
                     /locus_tag="PLT31_02135"
     CDS             complement(10009..10815)
                     /locus_tag="PLT31_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HPW93964.1"
                     /translation="MTIFLCSLLLSASMVTLLGVFRVISVEKSLYTVEAFLKDSVSED
                     SVASIKSDLYKIKWVDSVEYVSASAALEDFKKHFSEEMLSLVSDNPLPASFRLTLQDR
                     YKTPYYLNTLSAELNSMSLFDAVQAPVEWVERISSWKFSLLFWPFSISFLLLTTLSLI
                     IGNSVRLSLFSRKLLVENMKYAGGSSFFIVFPFVLEGLIQGLVGSLLSVFFFIFLFNS
                     LQNLLPVAAVYLSGYGWMLFLVVLLVTFLSVYFSWRSVHRFLLSDRAGGT"
     gene            10940..11899
                     /locus_tag="PLT31_02140"
     CDS             10940..11899
                     /locus_tag="PLT31_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93965.1"
                     /translation="MKHIGLKIIAFICGIALWLYTISLKDYSIEIEVPLILSRLPENL
                     ALVTKPPETIEVRVEGKAFELIRLRTLDANAAFISLDLHNVPLGLQRFEISSNLFSSP
                     NFPNIRYLGPTETSFLELDFDTKISHTVPIKLAVEIQLDSGFMLMKEPTLEPSELTIH
                     GARKALMRVFEVQTVKDHLTKLRENQSFTLPLNLSHIPSNIETANTSVQLDLEIEPVT
                     RIKVNKVPVQLIGMYDRTKYSISPTEASVEITGGKKILDSLNNKDIELIIEFNRFAIE
                     DADSLSPTVRILKPIQSTRVQPEKFYLKTIAPDTTAASTGNTP"
     gene            11896..12903
                     /gene="tsaD"
                     /locus_tag="PLT31_02145"
     CDS             11896..12903
                     /gene="tsaD"
                     /locus_tag="PLT31_02145"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPW93966.1"
                     /translation="MIWLGIESSCDETACAVLEDNPVHILSNPLYSQIEEHNLYGGVV
                     PEIAARAHLQKIAVIVQKAIEDAKISLADIGAIAFTAGPGLMGPLLVGASFAKGLAKD
                     LGIPAYGVNHLEGHLSAAWLTNPNLKPPFLTLTVSGGHTELVVENPNFEYTLLGRTRD
                     DAAGEAFDKCGKLLGLPYPAGALIGKLGASGNRKFHTFPRALKHEDHGEFSFSGLKTS
                     VLRYVENNSPEFIQENINDICASLEEAIVDILVSKTIWALKKTQLKTLIVGGGVSANN
                     HLRAKLKAYCDRKQIDLYFPSPQLSTDNGAMIAAAAIRRAKEHALLNISEVKPWMPLA
                     P"
     gene            12973..13404
                     /locus_tag="PLT31_02150"
     CDS             12973..13404
                     /locus_tag="PLT31_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93967.1"
                     /translation="MQKKKPLFFDVTGMSFVILVILLLSSFVSAQDTTLYQPQKKETK
                     IEIQDTSKSNNIILNVDIFGEAAIGSYYVLWSELDLSEEMKTLLTTRDFARDEFFMQN
                     IDREQFEQERMLQLFEDKKKEYFAIFPEEALKALEDEERDK"
     gene            13410..14192
                     /locus_tag="PLT31_02155"
     CDS             13410..14192
                     /locus_tag="PLT31_02155"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HPW93968.1"
                     /translation="MPFLFDQDKPFSSWTLQGLYGAPTLLFENIESTHLYLHNHFREL
                     YPGTLIVANSQTAGHGRHNRPWVSPVNKNLYFNLFIPLAGIQESYFSQITQIAAISLA
                     QILKNYNIQVAVKWPNDLLWNRQKICGIISERLIFNKESFLSLGIGLNVNTDPSDLES
                     IGRPATSLKIITRKTINRSYLLQLFISKMGSALLKLKKEGFLPWKNEWLQMENFLGKK
                     ACVVEFGNSISGTIYNINDDGSLLFQKEDGEIITVYAGDLEI"
     gene            complement(14168..>15836)
                     /locus_tag="PLT31_02160"
     CDS             complement(14168..>15836)
                     /locus_tag="PLT31_02160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPW93969.1"
                     /translation="SNVMQEIRRHKYTTSFLTVFHEALLGHKPTLLMNLSMSKDYRPL
                     LEDITSIAKANKLDGEEKHLQIIQAIDQMFLKMNRLIVKRVQKQKKAEEKENWTIPLD
                     VDSFVKQFEIPSHWRSLIHHEDSSKNMKSFNLNAWFDQLSFPTLAASLLLGARFASTR
                     IMNNDRETLQNFASKIGADCPEERVLWLTDTLFDKNGVASVLQETLKEIRRRELPIDL
                     LVCNNDLSPEDHLQVVPSLGSFSLSQFSDFKFNVPNLLDIHRIFQEGAYDRIVVSTEF
                     FMGGVGLFLQKAFNVPVYLFMHTDWMDYIKTNTELDPSNLDRIRRFCRAFYSQFDGIF
                     VLNSQHKNWLTSPAMGISSEKIFKTAHWNGSHFKPFDNKKEVNKESPVFLYVGRLSEE
                     KGVFDLPLIFSNIKEVFPKAILQIAGTGNAEKELKEEMPDAVFLGWVDHQDLPSIYSG
                     ADMLLLPSYFDTFGCVVTEAMSCGLPVAAYDCKGPKDIISNGVDGVLGADKESLAKAI
                     IEVFKNGKAWEMSRAALEKASLYQPEEIMNQMMLDLKLKSQISKSPA"
BASE COUNT         4988 a         3249 c         2727 g         4872 t
ORIGIN      
        1 cacttatgct ttagcattta gtgagaatga tacccctgtg gtagtgatac ccttgcggta
       61 caaatgacgc ttgtctcttc atgaaaaaag cactaactag gttagatgga gcgtttttgg
      121 taggcgaact agcgcgatat ttttttgctc tttttataaa aatgagtttc tataggtatt
      181 cttaagctat tcttaagctt tttttttcat agaaagttat tttgttgttt tgtatattgc
      241 accataggtg tattttacat tttaggtaat actaaaaaag catttaacga gtgactcttt
      301 tgaaaaaaat tcttttcagc ttattggtta caattctttt aacctccacc ttagaggctc
      361 ttcctattta tgggaatcaa agtcaccttc gttggaaaac ggtttcttca gaacacttta
      421 tttttaatta cccctcagaa tacacagaac acgcgggtat tgtggccgcc tatgccgaag
      481 ccgtttacga ttctattgtc aaacgttata gaacggatct tcataaaaaa gttaatgtaa
      541 atcttcatga cgctctttac gcgaatggaa atgcaatccc tacagaaaac actatcaatc
      601 tttggttaac caactgggat tttaagctca ggagttccca tcactggctg agcgacgtta
      661 ttactcatga attttctcat ttagtcagta ttgaaaatgg aagtaaactt cctccttttg
      721 tttatggatt tcaagtttct tattccgact attataatga aaacaccacg tctaattttt
      781 taagcatgtt cccttttact ctccaaccat tatggcttgc agaaggaaca gctcaatttg
      841 aatctgctcg aatgggtttt gacggttggg actctcatcg tgatatgctt ctcagaacag
      901 ctgctttaga agaggatctt ttaagtttag agtttatgca ttcctttgca aacaacgcct
      961 taaaagcaga acttgggcca tatactcaag gtttttcttt aatacgttat attgcttctc
     1021 attatggcga agaaaccatt tccaaaatat ggtctgagat gagtcgcata catagagtca
     1081 ctgtggatgc agcgatacaa tcggttcttg gaatcagtga aaaagaactg tacgaagcat
     1141 ggaaaagaga aattactgaa caatacgaaa aacaaaaaga atctcttggc ctattagtca
     1201 tgggagaaaa atggaccaca gatgctttct atcaagattt tcttgtcgtt gcagggaata
     1261 gtctttatgg tatttccaat tttggaggcg actggtttga tggttcgtta ttcaaaatgc
     1321 caaaaacaag agactccctt acggacgaac aaagtaaaaa agatagccta gtcattctgg
     1381 atttcgaagg tactattgat atcaaggatt attcaaaatc tggtttcaaa ccaaaaaaag
     1441 cttggtttga aagaggcttt agtattcgag aatcgaaaga caaagggccc ttactagctt
     1501 atgttcatta caagaataaa gatcgaaatg ggcgcactta ttttgacctt gacttattag
     1561 atactaatgg aaaggtagag cacatcacct cttttgccga tgctgtttat ccagatattt
     1621 ctcctaacgt taatgaaatt gtttttgctc gaagacttcc aaattcttcc cgttttgcat
     1681 taagccttgc tcctatttca aaagaagatg attcctatga tgaagaatat gttgatattt
     1741 ttattccaga agattcatta ttttattaca atatttatag tcctaaattc agtcccgatg
     1801 gtaaaaaaat cacctttagc ttttttgata ataccactcg cgggatcgct gtcattgaca
     1861 gagatgggaa aaacctaaaa ttattctctt ccaaaaatat tgattcccgt gatcccaact
     1921 ggatcgattc ggaacgcatc atttattctc atgacggcaa cggcattttt aatctttatg
     1981 agctgaatct tgccactggc aaaaccaccc ctctgacgaa tgttctcggc ggcgccttta
     2041 ctcctgtcgt agattcttca accatctttt atacaggtta tgataaagac ggtttttcta
     2101 tttactcgct aaaattagat gaaccgactc aagacacaac tattcaaaag aaacccttgg
     2161 agctaaaggg aattaaacct caaaaaaata acaaagaaat agagttactt gacatcactc
     2221 tccatggcgt tgaaacaaac tacaaaccaa ttccaacaat ccctcttcta gtacctctca
     2281 ttagctttga agaaagagcc cctgatttca cggtcgcagg cgatggagaa gtggttccaa
     2341 aaattggcct aatcatgctc ctttcagacc ctctaaaaaa gaatctttta caagtgggtg
     2401 gattaattga aatttcaaaa gactttgact atttcaactc aagtggctta aatcctagtt
     2461 acggtagaga cttttttgct ttactcgaaa acagaagctt ccctatcact cttcaattga
     2521 attacatgca ttccaattat gtaaacaaag atactgttcg ttacgaagat ccaagaagct
     2581 atgaagattc tatttctact agtcattatg cggtaccagt gcattcattt atgggaggtt
     2641 taggttatag tctttttaag aaaaatgata gtttgactat ttctcttggg tatgattggg
     2701 ctgactttaa tttatatgaa gatgatctcc gctggactta ccataaaaga gttaatgcag
     2761 gtgtttcttt aagtcactat agcggttttt cagaagacaa ttcaaatgca atttctggag
     2821 aaggaaacgg aatttcttta agctaccaat acagtaatgc agatctttat cgtcctggta
     2881 catttgcaga aagctttacc gtgagcgcga gtggcgccat caagcccata tacagaaact
     2941 ttcaaatcca tgaattaggt ctttctcttt ttgggagctt agataaccct attcactctg
     3001 gagctcgttt tgccgctggg gccaatgttt ctggaattct ttcttggaaa agcaaaaata
     3061 aaagcgatac cttagatgcc ttctattatc acccactctt tctggaaggc tacccttact
     3121 taatttccac agaagatttt gctcgaagcg gcacaaaaac ggctttagct cagctacatt
     3181 atatttttcc catttataaa gactttagga agcatttatg gatttttact tcacgtgatt
     3241 ttttcatcga tgttttcgct caagtcggca ctgcttggaa ttcctcatgg tttgataaag
     3301 aggccttaaa aaacatagac ctatgggatc gaagtgttgg ttttgaaata cgtttagcaa
     3361 ataccctatt ctactcgatt cctttagata tttccttaag cttcgcaaga ggtttagatc
     3421 gtattgctaa aaaagcagac ggaagtggtg gttctaggtt acataggatc gatattcctt
     3481 tacttcctac cgctattgaa cctactaaaa tcagctttac tataggaatg ggttttaata
     3541 atcgttggat gaaataacat gaaaaaaata gcgatcattg gagcagggct cactggtttg
     3601 accactgctt tttacctaag aaaaaataac attgacatta ctctttttga atccactcaa
     3661 cacattggtg gcgttattca aacaaaagaa aaagatcagt ttatctggga aacaggccct
     3721 agtactggtg ttcttggaaa accagaagcc atggaacttt tcgaagattt agaagcctta
     3781 gatattttag aaacagctcc aagtcttgca gggaaacgtt atatctggaa aggcaaaggc
     3841 ttacaccctc ttccaagtgg cccaataagc ggattattaa cacctctctt ttcttggaaa
     3901 gataagttcg gcctattatt tgaacccttt cgccctaaag cagaaaatcc tgatgaaaat
     3961 ctttcttctt ttgtgcgccg tcgtctcgga gaatctgttc ttcaatatgc tgtagatcct
     4021 tttgtttctg gtgtgtatgc tggttctcca gataccatta ttccacgcta cgctttgcct
     4081 aaactctaca atctagaagc cacctacggt agttttctcc gtggtactta tcataagatg
     4141 aaagagccga aaacacctag agatcttaaa gccacaaaaa aaacattttc tgcaaaaggc
     4201 ggactttctc gtcttatcga acgcttagta gctgtcattg gtgaagaacg aattaaaaca
     4261 aaatgcaaag acctcgttgt catgaatgaa gaaaaaggct tttctgtttc ttctagtgct
     4321 tttgaagcag aacactttga cgaagtcatc tttacaggat cagctaaagg gattctaaaa
     4381 acgttccctt tcttacagaa taaaggccta gaagccattt ctcaaatacg ctacaccgct
     4441 gtagctgaag tcgctgtcgg ttttgaaaag tgggaaggtt ttcctcttga tggttttgga
     4501 gccttaattc catccaaaga aaaaagacgt attttaggta ttttgtttat gtcttctctc
     4561 tttcaaaata gagcccctaa gggtggcgct atgctatcgg tttttgttgg aggagaacgt
     4621 catcctgaat acgtttcctt aagtgacgat gagctgaaag cactagttaa agaagagtta
     4681 attcccattc ttaaaattcc taatttcaat cccgctattt ttgaaatcag tcgtcatcaa
     4741 gaagctattc cacaatatga tctactaact ccagatcgta ttaacgcctt taaggccgca
     4801 gaagaaaatt tccctggact ccacttagga gggaatggaa ttggtggaat cggtatggct
     4861 gatagaataa aacaagggaa agaacttttt ttatcaatta tttcataaat aataaagttt
     4921 tttatccttt tttctattct tttttctata ttaagcgcgt tccttcctcg cccgggtggt
     4981 ggaatggtag acactgggga cttaaaatcc cctggtcgca aggccgtgcg ggttcaagtc
     5041 ccgccctggg catttttaca gaggcaactt atgatagaat tctatccgca aagtatctat
     5101 tacccccgcg aagcggtgga agccaaattt gcaagcgggg aactccaagc aactgaaaaa
     5161 cgtttgcttg aatttgcgga gaggcaccgt tctgcaattt gggagtcggc tttggaggat
     5221 tcttctgagc ctactgatgc tattttgcta gataatctaa gagccttttt attatctatt
     5281 ggatctattc atcctgcttt tgaaatgggt gaacttatca aagaaataaa aaaagaagtc
     5341 tggtatcaaa acgaaaccca agacgtatcg ccaaatgaaa ttgctgaaaa ctggaaaaaa
     5401 acttatgcca ttaaatggcg tgaagctcgt atgtttgaag catttattct aattgaacgt
     5461 tgttcaaaag agatcttgaa tattttgcgt gccactggaa aggtataagc aagctcgtag
     5521 agcccttatt cgatttttta tatctccaat atttttctac aaaatgcact aaaggtttat
     5581 ttaatgcatt tttttctgca ttattaaact gtacttttac atttttaaaa aagccatctt
     5641 gatctatttc tacccaaaga ccaatcaaaa gatcttcttt taatctcttc cttccagaat
     5701 aaatattttc atataaataa tgaagagaag ataaacgccc ttgaagatac aagagaaggc
     5761 tttctatatc tctttctgcc gtattttcaa aaacataaag atcagaaatc gtcagaaggt
     5821 ttagcctccc atggaacaat acttctgaag gagcaggttc tggagtttct ttcactaatg
     5881 gggcaatttg aggagaggac ttccaaaaaa gaattgctaa aagccccaaa aacaagccta
     5941 gaaagaaaat gacagaaacg tttctgcgta tcattatcgt tcaaactcca tcgctttaat
     6001 cgctaattga atagagcgtt ttcgattata tacattccaa gtcggttcaa aaacaaccgt
     6061 ggcttcaaaa ttattctgtt caattttgct tttttgattt cccattccaa aagcaattgc
     6121 gctaaagcgg cgactcccat tctgattgat ttccatttgc aaatggttcc cgcgcaattc
     6181 tcttagctta tgaatcttaa ccttttctgc cttaaagaca ggatagggga aactcccccc
     6241 ataaggttcc attttcaaaa tccaatccat caccgcttca tctaattcag ataaggcgac
     6301 taaaatatca aaagatgttt tttgctcgca ttcatcaggg aaaaaatttt gatttgctgc
     6361 aaataaaatc aactgctctc ggaaagattc tatttctgaa actggaatag aaaagccagc
     6421 ggcattcgca tggcctcccc aacgatcaaa taaatgtcta aattcaaata acgctttgtg
     6481 ccagttaaaa ccaagaacag agcgtgcaga tgcatgcgcc gttccatcag gttgcagaga
     6541 aagaatggcc gtgggacgac taaattcttg tgccactttt gcagccacaa tcccaataac
     6601 acctaaatgc caatcgtttc catcaataat caataccgaa ggaatatcat ttccataacg
     6661 agaatgaacc caacgaatcg ccgcttcaga tatttccgct tcttttttct tgcgtaatac
     6721 attccactct tttaaagagg caagaagcga aggggctctc attggatcat cacataagag
     6781 caaatgtaat gcagcgtcag cacatcccat tcgtcctggg gcattcaaaa taggggctat
     6841 acggaacaaa acatcttgcc caccgataaa agaatgcatg tttaaaacat cgcggcataa
     6901 ttcttgaacg ccataccaat cactatgaca cattttttta aggccagaac gagtaagcat
     6961 tttattttct tctgtcaaag gcactaaatc agcaagcgtc cctaaagcca ctaaatccaa
     7021 aaacttttca gcacttgaaa gaccaagtct tgagaacaaa gcacagatta acttataaga
     7081 taatcccacg ccgcaaagtt cttcattttt ataggaatct tcttctcgat gtggatctaa
     7141 taaaacatca catgcaggga gcccatcacc agaaggctga tgatgatcga ctaccatcac
     7201 ttgaagtccc tttgattttg catattcaat ttcagtaacg gcagtgatcc ctgtatctac
     7261 ggtgataata tttttcgctc ccgcttcaat catctcatca atcgccgaca ctgataatcc
     7321 atatccagag ctaaaacgat ttggaagacg ccattcagta gcgattccta tttcttttaa
     7381 gcctctagtt aaaagagcaa cactactcaa accgtctaaa tcataatcac caaaaataaa
     7441 tatcttttct tggcggtcac gaacagcaag aacccaatct acagcttcat tcatatcgag
     7501 catgagaaat ggatcttgaa ttccattctt ttcgccaaaa agcatgtaat aggcttccgc
     7561 gacagaagtg catcctcttt tcactaaaag ccgagcgata acatgaggta cttttaattc
     7621 agaggtaatc gtagaagcga gtagttcttg catattacct aaatccatca aaaagtttta
     7681 tggctaacga ctgaaggcaa actaaagcac ttgttcttcg agtaccaatg cgatacattg
     7741 tttcttgaac tgattgtaaa gccactgtat aggcatttaa atcaagaggg gtattctctt
     7801 tatccttaaa ttgtctcgtc gtttcggcta aacgaagtgg cgctccagac atatcacgaa
     7861 cgatatctgt aatcaaaaag cttagcactt ctaaaaataa aatggcaacc gcagggtctt
     7921 ttaattcttc ggaatccaga ttaaaaaaca aatccgaata atttccagag atggatgatt
     7981 ctaaaaattc taaagctaaa tcttgtaatt ccgtcgcttt agaagcataa taaatcgcac
     8041 gccccacaga tccttgagac aaaccaagaa catcatctga aattggaata tccacatatt
     8101 gagaggctaa atctcgcact tcttcagaag ataaaggagg caaatgaata gacaagcagc
     8161 gcgaacgaat cgtttgtaac aactgctctt ttgaagaagt tgtcaaaata aaataagtat
     8221 taggaggcac ttcttccaac gtcttcaata acgcattgga agcagactca ttcatacgat
     8281 ccgcttctgc aataatcaca catcgcacac gatcattttt taaggaaaag cgcccagtta
     8341 atgatcgtat taaatccaca gagatttctg ctgcggcaga caaataatca atactataag
     8401 ggttttcgat gattttttga atgtacacct gagtaaattc ttccaccgtt ttagcggaac
     8461 tcccttcaga gacatcggaa gctttttttg ctttagcttc tttactttct aaaggaatca
     8521 cccaattttc tacagcacca gaattgacgc tcatcttaca accaaaacat tccccacaag
     8581 gtcgctgtga ggcattcgta caacaaagag cctttgataa ttccatggcc aatgcctttt
     8641 tcccaatccc tggaagacct tcaattataa ccgcttgagg aaagtggttt tcttttaaag
     8701 aagcccctaa acgtattcgc gtacgttgtt gagaaagagg accttttagc tcatattcaa
     8761 tcatttttca ttcaaccatt gtaaaggatc tatcgtttga gtaccttgac tcacttgaaa
     8821 atacaatttt attccattca aagaggcgag atctcccact tccccaattt cttctccatt
     8881 tcgaaccgca tccccttctt gtacccgaat ggaattcaaa tgcccataaa cagagtaaaa
     8941 accgccttca tgttctataa tcacggaagg gccgcgacca tcaatttcag ccgtcatcac
     9001 aactttccct ttagcagccg ctttcacctt tcctccacgt tggcctttta tttcaacacc
     9061 taagtttcta gtcatgatat gaagcacagg atgctcttgc atgccataat tagaaataat
     9121 aggcccatgc ataggcattg tttttggccc aacaatagcc ttgtctaaaa caaccgcagg
     9181 agtttccttt ttaacttttg gtttttcttt ctgaaccttt ttcgacttct tctttttttc
     9241 ttcagctaaa cgcttctttg cctctgcttc ttcttttttt cgtttctgtt ctaatttctt
     9301 gattaataaa atcattgtct tttggtttcg ttcaaattct tctaaaacct ttttctgaag
     9361 atttttatct cgctttaaca taaacaacat ttcactctga cgttcaatct gaatcacaaa
     9421 tcctttttct tcagatgctt ttttggatcg taatacctgt aagtttttta gatgttctgc
     9481 ttccatcgcc ttcttttcag tacgttcttt tatggacgca cttaaatttt ccacaagcat
     9541 tttatcttcc gaaagaaggc gcctcactaa atacaattgc ctttcaggat tttctttttg
     9601 acgaagtaaa cgatagagtt cctccccttc actatgtcgt ccttgaatat ataaggtacg
     9661 aattcttttt ttcatagcat cttgttgctt agatattttc tgatctagag aatctaattc
     9721 aagagataaa cgtttgacag ccgcggcaac taaagattca ttggaagcaa gcatcgatac
     9781 atactctctc gtttgtgata aattttgatc gagtagcgat agtgtattta aaacactttt
     9841 ttcttctgtt tctaagagca ttaattgctc tctctttttt actaaatctt tttcaagttg
     9901 ttttagtgct ttttgctgat tccgaatttg agtatctgtt ttcaagggag ccgcgcttcc
     9961 tatactcacc aaagcacata acaaaaagca caagattctc attctttctc aagtgcctcc
    10021 agcacggtca cttaaaagaa agcgatgtac agaacgccaa ctaaaataaa cagataaaaa
    10081 tgtgactaat aaaacaacta agaatagcat ccagccatag ccgctcaaat aaacggctgc
    10141 aactggtaaa agattttgta acgaattgaa taagaaaata aagaagaata cagaaagcaa
    10201 actaccgact aagccctgta ttaaaccttc aaggacaaaa gggaacacaa tgaaaaaaga
    10261 gctgcctcca gcatacttca tgttttccac taaaagcttc cttgaaaata aagaaaggcg
    10321 tactgaattc cctataatta aagatagggt tgttaaaagt aaaaagctaa tagagaaggg
    10381 ccaaaacaac aaagaaaatt tccaagaaga gattcgttca acccattcta ctggagcctg
    10441 aacggcatca aaaagagaca tactattaag ttcagcagat aacgtgttta aataataagg
    10501 ggtcttatag cgatcctgta gagtcagtct aaaagaggct ggcaatgggt tatcagaaac
    10561 taaagaaagc atttcctcag agaaatgttt tttaaaatct tctaaagcag cagatgcact
    10621 aacatattcc actgagtcta cccattttat cttataaaga tcagacttta ttgacgccac
    10681 agagtcttct gaaacagagt cttttaaaaa cgcttccact gtatacagag acttttctac
    10741 cgaaatcacg cgaaaaacgc ctaaaagtgt gaccatactt gcggaaagaa gcaaagagca
    10801 aaggaaaata gtcaccaagg acggcaaaat aaccgtccgg tgctgcttta aactacggaa
    10861 agattccgat attaaatatc ctatttgact aaacaccgct tgaatataac taaattttga
    10921 aaaaccaaat tggctattta tgaaacatat cggattaaag atcattgctt ttatttgcgg
    10981 aatcgcgtta tggctctata ccatttcatt aaaagactat tctattgaaa ttgaagttcc
    11041 tcttatttta tcaagacttc ctgaaaattt agccttggtt acaaaacctc cagaaacgat
    11101 tgaagtccgt gtggaaggta aggcttttga attaatccga ttgcggactc tggacgccaa
    11161 tgccgctttt atctctttag atcttcacaa tgtacctctt ggacttcaac gatttgaaat
    11221 ttcatctaac ctctttagtt ctccaaattt tccaaacatt cgttatttag gcccaacgga
    11281 aacctcattc ttagaattag attttgacac caaaatatct catacggttc ctattaaact
    11341 tgctgtagaa atacaactag attcaggctt tatgttaatg aaagagccca cccttgaacc
    11401 aagtgagctc accatacatg gggcaagaaa agcactcatg cgagtctttg aagtacaaac
    11461 agtaaaagat catttaacaa aacttcgaga aaatcaatcc tttactttac ctttaaatct
    11521 tagccacatt ccttctaata ttgaaacagc aaatacctct gttcaattag atttagaaat
    11581 tgaacctgtc acgcgtatta aagtgaacaa agttcctgta caactcatcg gtatgtacga
    11641 tcgtacaaaa tacagtattt ctcctaccga agcttctgtg gaaattacgg gaggaaagaa
    11701 aatcttagat agtttaaaca ataaagatat tgaattaatc attgaattta accgctttgc
    11761 tattgaagat gcggatagtt tatctcctac tgttcgaata cttaagccca ttcaaagtac
    11821 aagagtacag cccgaaaaat tttatttaaa aacaatagca cccgatacga ctgctgcttc
    11881 aacaggaaat actccatgat ctggcttggt atagaatcta gctgtgatga aactgcttgt
    11941 gctgtattag aagataatcc agtccatatt ttatcaaatc ccctttatag tcaaatagaa
    12001 gaacacaatc tctatggcgg cgtggtccca gaaattgcgg cacgcgccca tcttcaaaaa
    12061 atcgctgtca ttgttcaaaa agcgattgaa gatgcaaaaa tttcactcgc agatattggc
    12121 gccattgctt ttacagctgg ccctggatta atggggccac tacttgtagg ggcaagtttt
    12181 gcaaaaggcc tcgctaaaga cttaggaatt cccgcctatg gagtcaatca tttagaagga
    12241 catctcagcg ccgcttggtt aactaaccct aatctaaaac ctcctttttt aactctgact
    12301 gtatctggag ggcatactga attagttgtt gaaaatccta attttgagta taccctgctt
    12361 ggacgaactc gagacgatgc cgctggagaa gcctttgata aatgcggcaa attattaggc
    12421 ctcccttacc ctgcaggagc cttaattgga aagcttggag cgagtgggaa tagaaagttt
    12481 cataccttcc caagagctct aaaacatgag gatcacggcg agttttcttt tagtggacta
    12541 aagacctctg ttctccgtta tgtagaaaac aactctcctg aatttattca agaaaatata
    12601 aacgatattt gtgcctcttt agaagaagcg attgtcgata ttttagtttc caaaaccatc
    12661 tgggctttaa aaaagactca attaaaaacg cttattgttg gaggtggagt tagtgccaat
    12721 aatcacttac gagctaaact caaagcatat tgtgatcgaa agcaaatcga tttgtatttt
    12781 ccatccccac aactcagtac tgataatgga gcaatgatcg ctgctgcagc gattcgacgt
    12841 gccaaggaac acgctctttt gaatatctcg gaagtaaagc cttggatgcc tttagcccca
    12901 tagtttttac agaaaaaaaa cactatatta gtaaagcgag taaaataaga attgtctata
    12961 gggaaccgta ttttgcaaaa gaagaaacct cttttttttg atgtaactgg aatgtctttc
    13021 gtcattctcg tgatacttct tttgtcttct tttgtttctg cacaagatac cactctctat
    13081 caacctcaaa aaaaagaaac aaagattgag attcaagaca cctctaaaag caataatatc
    13141 attttaaatg tggatatttt tggtgaggca gctattggtt catactatgt gctatggagc
    13201 gaacttgacc tttctgaaga aatgaaaacg cttctcacaa caagagattt tgcccgagat
    13261 gaattcttta tgcaaaatat tgaccgcgag caatttgaac aagaacgtat gctccaactt
    13321 tttgaagata aaaagaaaga atacttcgcc attttccctg aagaggcctt aaaagcgctc
    13381 gaagatgaag agcgagataa ataacatcta tgcccttttt atttgatcaa gacaagcctt
    13441 tttctagttg gactcttcaa ggcttatacg gtgctccgac tctccttttc gaaaacatcg
    13501 aaagcaccca tctttatctc cacaatcatt ttagagaact ttatccaggc actcttattg
    13561 tcgcaaactc acaaactgct ggccatggca gacacaatcg tccttgggta tctccagtca
    13621 acaaaaattt atacttcaat ttatttattc ctctcgcagg cattcaagag tcttattttt
    13681 cgcaaatcac ccaaatagcc gctatctctc tagctcaaat tctaaaaaat tataacatcc
    13741 aagtcgccgt taaatggcca aatgacttat tgtggaatcg acaaaaaatc tgtggcatta
    13801 tttctgaacg gctcatcttt aataaagaat cttttctctc tttaggaatc ggattaaacg
    13861 tcaacaccga cccttctgac cttgaaagca ttgggcgccc agcaacgagc ttgaaaataa
    13921 tcacccgaaa aacgatcaat cgttcttatt tacttcagtt gtttatttct aaaatgggat
    13981 ccgctctctt aaagctcaaa aaagaaggct ttcttccttg gaaaaacgaa tggcttcaaa
    14041 tggaaaactt tcttggcaaa aaagcttgcg tcgtagagtt cggaaactcc atctcaggaa
    14101 ccatttataa tatcaatgat gatggaagcc ttttatttca aaaagaagat ggagaaatca
    14161 tcacagtcta tgctggcgac ttagaaattt gagatttcag ttttagatca agcatcattt
    14221 gattcataat ctcttctggt tgataaagag aagctttttc tagtgccgct cggctcattt
    14281 cccatgcctt accgttttta aatacctcta taattgcctt tgcaagcgac tccttatccg
    14341 cacctaacac cccatctacc ccattagata taatgtcttt aggcccttta caatcataag
    14401 cagcaacagg gagtccacaa ctcatagctt ctgtaacaac acagccaaaa gtatcaaagt
    14461 aactaggcaa taaaagcata tcagcaccgc tataaatcga aggcaagtcc tgatgatcaa
    14521 cccaacctaa aaaaacagca tcaggcattt cttcttttaa ttctttttcg gcatttcctg
    14581 ttccagcaat ttgaagtatc gctttaggaa acacttcttt tatatttgaa aaaattaatg
    14641 gtaaatcaaa tactcccttt tcttcactta atcgccccac atataaaaag acaggagatt
    14701 ctttattcac ttctttttta ttatcaaaag gcttaaaatg agaaccattc caatgagctg
    14761 tcttaaaaat cttttctgat gaaatcccca tggcaggact agtgagccaa tttttatgct
    14821 gcgaatttaa gacaaaaatc ccatcaaact gactataaaa agccctacaa aagcgccgaa
    14881 ttcgatctaa attagaagga tctaattctg tattcgtttt aatataatcc atccaatcgg
    14941 tatgcataaa taaatagacg ggaacattaa atgctttttg aagaaagaga cccactcctc
    15001 ccataaagaa ctcagtactc accacaattc gatcatacgc cccttcttga aaaatccgat
    15061 ggatatccaa aagattcggt acattaaact taaaatcgct aaattgagat aacgaaaagc
    15121 ttccaagaga tggaaccact tgtaaatgat cttcaggaga aagatcatta ttacagacta
    15181 acaaatcgat tggtaattcc cgacgtctaa tctcttttaa cgtttcttgt aaaacactgg
    15241 ctactccatt tttatcgaaa agtgtatctg ttaaccacaa gactctttct tcaggacaat
    15301 cggcccctat tttagacgca aaattttgaa gcgtttctcg atcattattc ataatacgag
    15361 tacttgcaaa gcgagccccc aaaagaagag atgctgcaag tgttggaaat gaaagctgat
    15421 caaaccaggc gttcaaatta aaagacttca tgttttttga cgaatcttca tgatgtatta
    15481 aggagcgcca atgacttggt atttcaaact gctttacaaa agaatcgaca tctaacggaa
    15541 tcgtccaatt ctctttttct tctgcttttt tttgcttttg aacacgtttc acaatcaatc
    15601 gattcatttt tagaaacatt tgatcaatcg cctggattat ctgtaaatgt ttctcttctc
    15661 catccaattt atttgccttt gctatagaag taatatcttc taataaagga cggtaatctt
    15721 tactcatcga taaattcatt aaaagcgttg gtttatggcc taataacgct tcatggaata
    15781 cagtcaaaaa acttgttgtg tatttgtgtc gacgaatttc ttgcatcaca ttagat
//
LOCUS       DAOOBB010000024        15814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_4834_length_15814_cov_4.229139, whole genome shotgun sequence.
ACCESSION   DAOOBB010000024 DAOOBB010000000
VERSION     DAOOBB010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 15814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15814
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4834_length_15814_cov_4.229139"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PLT31_02165"
     CDS             <1..254
                     /locus_tag="PLT31_02165"
                     /inference="COORDINATES: protein motif:HMM:NF013089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HPW93970.1"
                     /translation="EAIGSTMLLWYGGFFISLSISMALLIKVTQKLPIGTAYAVWTGI
                     GAVGTVLIGILIFKEPATWTRIFFLTTLIISIVGLKAVS"
     gene            313..480
                     /locus_tag="PLT31_02170"
     CDS             313..480
                     /locus_tag="PLT31_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93971.1"
                     /translation="MNSDSLKQKIQGFLAKIKGLSRTQKVIILFVMVLVPAGIAISAF
                     LILYFKGKKKR"
     gene            477..1625
                     /locus_tag="PLT31_02175"
     CDS             477..1625
                     /locus_tag="PLT31_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPW93972.1"
                     /translation="MIFSSRLPKDLSESPFFKALEEEKQKSPYIDLTVSSPLKAGILS
                     DISFEYIEKKWTSKEVSAEGSLLVRQAICDYYASRFGVFSPEQVIISSGTSEAYSFLF
                     KTFCDPGDVILTPMPGYPLLDTLANLEYLSCYPYFLDYNEESLSFSLDSQTLLSAPER
                     AKILLLVSPHNPTGHVVSELEWKQIISFCEERSLVLVVDEVFGDYCYDGKPRSWSYDC
                     GTVPVFWLNGLSKTVGNPHLKLSWMVFKAPEDQFEKIREALTFVADAYLSVSSGAQAA
                     ALDLLPKAGSIQERVVSRLKQNLDALKAMFPHAPIVQGGWYASICCDAYDDESLVLEI
                     LKENRLLVQPGFFFDFSEDGWLVISLLSSPEDFLFGLKTIAPFVNQNQ"
     gene            2500..3339
                     /locus_tag="PLT31_02180"
     CDS             2500..3339
                     /locus_tag="PLT31_02180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02147 family protein"
                     /protein_id="HPW93973.1"
                     /translation="MNSVILYENYRAYIQDYYSEHHERFGFTWRRFAKAAGYSSPVFL
                     KLVSEGKSNLSDLGIERTAQAMGLVGNDLLYFRVLVHFNQEKKASVKKNYYNEMRAIA
                     SKSQVSLVGEDQYDYFSSWRNPVLRELADSLKGLSPSDYASLFVEKTTPEEVKKALKI
                     LLKTGLMTQPSPKEYEKTEAALSTGNLEVASLTIRDMHRQMGELAVKSLDDVDPQERD
                     FSGLTFGVTEEAVERIKAEIADFRRRIMSIVLEDKGFDRVLRLNMQLFPLTKQAKKER
                     EKQ"
     gene            3336..4730
                     /locus_tag="PLT31_02185"
     CDS             3336..4730
                     /locus_tag="PLT31_02185"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HPW93974.1"
                     /translation="MKTFNAIFWIMCVAGALFWACTTNVAGGVTEETNSIAGVIYNSE
                     QKVASGVEVLARSVADTTKLYKDTTDSKGSFAFKFKEQGLYGVSAKTSELAYYELVEY
                     KGVTLNVEASLNKYTSFTGKLTLSGAVYNEPIQVFIPGSPWAVYTNENLEFEIDSVPE
                     GSYYVYVRSPDQTRFNDAYFWVSLLPDTLKGSQGPLPTSLATSAPSDDKNAVYESENS
                     VFNFSWTIPMSSEYGLVGWWPMNYISKDSSSIQYISDARGRTDVGVVYGKAFLDSGVL
                     GQSLVLRGSNDFVVIESDRGVLDSATALTLEAWIYIEDLNETEDVTNYRKNLIGKVGF
                     GNSDDRSVFSLAIIKNECEVEGSPVLAFFIADGSGTSLDCANAVVDSNAIKVGEWLYT
                     TSVWNGQTLSLYVNGAFVASRGVSVDVLTPSIESLYFGKENLNFRLDEVRWSTVAISA
                     SDVLYRYYNQGGVQ"
     gene            4727..5254
                     /locus_tag="PLT31_02190"
     CDS             4727..5254
                     /locus_tag="PLT31_02190"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPW93975.1"
                     /translation="MMTQDLQEEIAFCEVNEHGRLLNANKRFCKLFGFAENDVAWHYV
                     KDLYRHEKDWDAYQKCSPERGSNIRFVARLKNRKGRSFKCSISRDVFQDDTGRIIYRN
                     RIQKLLESSKDTSDNYNGSSSNESSFVYLAKCVSCAKQLKISASHRDSRLPIYCPECA
                     AISFSNHFQSKEIAL"
     gene            5566..8109
                     /locus_tag="PLT31_02195"
     CDS             5566..8109
                     /locus_tag="PLT31_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPW93976.1"
                     /translation="MSDFSNESEKILGKAQELMDKKSHSYLGSIHLACALVDYPDARL
                     KNLYKSKNVKIKELRTRLDSILDSVPKVAGVNPEASPDSDLNRVLRAAIQAARQVSRT
                     ASPSDLFVALLRFASNRQLVKIFEDAVGSAEVVETWLSDPMCAAAVADEQSPLKLYGR
                     ELVELCAEGKLDPVIGREDEIRRVILILARKTKNNPVLVGDAGVGKTAIVEGLAERIY
                     KGDVPDALKGKKLFALDLSALIAGAKFRGEFEERLKAVLEALEEDGNTLLFIDELHMI
                     VGAGKTEGSMDLGNMLKPKLARGELHCIGATTTQEYRKYIEKDSALERRFQPVSVFEP
                     SEDEALSILRGIKEGFEAHHGVRLHDNALVSAVKLSIRYIADRNLPDKAIDLIDEAAS
                     FVKTQLDTVPEALDNLQRKELQLKIEEQALSKETDDKSAKRLSELREELALTSAAVSE
                     MQERWQEQRAKFSELKEAKSSLKKAKEEMEQAEAHYDLNKAAELKYNTIVGLENQVKA
                     LEEEARAASQNGELTQEVNEETIALVVSRWTGIPVTRLREGERSKLLLLEDRLHERVI
                     GQDDAVKAVAEAILRNRSGLSRENAPIGSFLFLGPTGVGKTELARALAYTLFDSEDAM
                     IRVDMSEYMEKHSVARLIGAPPGYVGYEEGGQLTEAVRTKPYSVVLLDEVEKAHPDVF
                     NTLLQVLDDGRLTDGKGRVVSFKNTIVLMTSNLGSHLFQEEHQKVSLEQVMPELRRFF
                     RPEFLNRLDETLVFTSLSRDQLHSIVKLKVNALQERLSKQGFLMSVTEEAVAAIAEKS
                     YDPQFGARPIQRYLQRELERVLSHAIIAGEVKTEIPFVVDYKEKTFVIQ"
     gene            complement(8135..8833)
                     /locus_tag="PLT31_02200"
     CDS             complement(8135..8833)
                     /locus_tag="PLT31_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93977.1"
                     /translation="MVKKLLFLLAFLSLYSHALDRMWDARFTLGPEIRTYDKMGFGIG
                     LRTGFHSDVNFPVNFQARLNNEIEIGGKLFLESPNSLEHIIGNIDLGAKYNLSYNSYV
                     GIDTYFALNRNYGGGLIFTYSQQTHVARRFSNLYEIRVGFLDGITGSDGYMKLQVGFM
                     PTLHIGKTFLAMVEMNSSGSIGNIQDDFMIDLLPKLELRIANVRLRLEVDIGILLEKN
                     NSQKQIALYTLIAL"
     gene            8876..9973
                     /gene="hisC"
                     /locus_tag="PLT31_02205"
     CDS             8876..9973
                     /gene="hisC"
                     /locus_tag="PLT31_02205"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HPW93978.1"
                     /translation="MIEARPELDKLSDYVPGKSIDEIRTRYGLSKVVKLASNENPLGA
                     SPKAMEAYMKIASSLHLYPRGDAPHLVEALASKYRVKHNQVIIGNGSDEVIDMVGKAF
                     IRPGDTCLSLEPTFSVYRSVTYSYSGHFASVNSLDQQEPLKELAKKIDEKTRIIFICN
                     PNNPTGGYFSEDEIVQFMVQVPRHILVFVDEAYAEFATAANYPSLIKMIDLFPNLLLN
                     RTFSKIYGLAGLRVGYAISSPQVISNLWKVKPPFDVNLAAQEAALAALQDTSHIKKTL
                     EVTAQGIEYLTKALSTMGYRVLPTQANFICVHIGADAKKMVSFLEEKGMIIRGLSSFG
                     MPEWVRITIGKHEENQFLLECVNLWRNNGRD"
     gene            9960..10763
                     /locus_tag="PLT31_02210"
     CDS             9960..10763
                     /locus_tag="PLT31_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HPW93979.1"
                     /translation="MAEIKELLEMALKASSLAEEQILKYYGSSYEVSWKEDQTPVTIA
                     DQKAEEVMRSFLEKEMKDVGFIGEEFGVENPKASYQWIMDPIDGTKAFIHGVPLFGTL
                     LALYKNGKPCVGLIQLPALKTCLWASKGAGAYVDGHQVHCSKVSSIEESLVLSGTINT
                     MEDKGLGESFKTLRRASKLYRGWGDCYGYYLVASGRAEIMVDSVVSLWDIAPMPVIFS
                     EAGGVFSTITGETSLFNNQGEPIHSIYEGYTGLGCAPSVYSKAQEILSE"
     gene            11029..11520
                     /locus_tag="PLT31_02215"
     CDS             11029..11520
                     /locus_tag="PLT31_02215"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HPW93980.1"
                     /translation="MKDNKYRSIVKLDFQYAHRFMNYPGEASYLHGHSGLLTLEIEGE
                     LDKHNYAYPVKEAQKLAWAVADNFMHATIFQEGDPLFDLVLQGYEKTGMKGTTNRFGT
                     PAKVLDHELVKSFPESRLVLSKGPSTCEWFCTVFYELLKDKLNIKKVEFRSSSLNCAS
                     KEF"
     gene            complement(11588..12769)
                     /locus_tag="PLT31_02220"
     CDS             complement(11588..12769)
                     /locus_tag="PLT31_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW93981.1"
                     /translation="MIKRFLKVTFSEWKLFFTDPAAILLLVIAGFIYAFYYPTPYLKQ
                     VVTEAPVGIVDLDKTAMSRQLIQMSRAAQQIDVKYVFDDIHQAKLSMADGNIYGYMII
                     PNNMERDIRTGQKVTLNVFTHGAYVMLHGNIGTAFATSGLTLGAETKVKRIALDKQVP
                     AKRAMAMRDPFPIQIQTLYNRTGSYSNYVVPSVLVLILQQSLIIGICTLGGARAHRKF
                     RKKKQESAAENESLPYRYFGRSLAYFLHYACFIAFYHFVVYNVFDFPRRGEPLPMIVF
                     AVVFLSASINLGMVLSQVFLRRETGMQIFLFLSIPILFLSNFSWPRYLIPDWMAALSA
                     ILPSSYAVPAWLSIQQMGAGIQDASVFLVKMGVQSVIYLGLGLLLSFLRDKSKLKTGD
                     M"
     gene            complement(12762..13817)
                     /locus_tag="PLT31_02225"
     CDS             complement(12762..13817)
                     /locus_tag="PLT31_02225"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW93982.1"
                     /translation="MILLVLPVSTSIFMVHFFSAESIQRIPIGIIHYDYSKLSERLEY
                     SLSANPVLDLKLHCSDFSECERGIRRGEIQAFIVIPYQFEKRVLRYETPVVPIYSNGQ
                     YYLTNMFALKEIRSVFSTIGSNLFTRHIKEPIKTEIHSVGNSTGNYQGYLGLGLVTSI
                     FHMSSILIAVYVFSFPLRDRKTKEYIDAAGGSRMILWFASVVPLILISWFLMLAIYTY
                     THRFLAPLTLNEFIMVGAGQLAMVLACTGAGISFVGITGNMRMATSVAGVIGGPAFAF
                     AGQTFPIIAMPFFVRCFAFLLPLTHLLKIQSQMILGDVGLIPSWDSLVILLGMGLFWH
                     LLGGRFMFMRWKKWNHD"
     gene            complement(13905..14894)
                     /locus_tag="PLT31_02230"
     CDS             complement(13905..14894)
                     /locus_tag="PLT31_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPW93983.1"
                     /translation="MKIVLKVLLILTLIFLIIAGITTLQKFATEPREEFLQGQMEARR
                     VLVAGKIPGRLERLFVHEGDMVVKDSLVAIISSRELEAKKLQAEGALGAAKAQSNKAN
                     TGARAEEITALKAMKERAQDAADLAKTTFDRVQKLYNDGVIPLQKRDEAETQMKTAVA
                     AATAAKAQYEQAVAGARVEDKAAANALVIQARGANAEVDAYLDETKIRAPISGEVSLK
                     LAEEGEIVGAGMPVIAITDLNDSWAVFHIREDFLKHISKGKTFTVQIPALDKTIEMVV
                     TYIASVGDYATWRSSKESGGYDLKSFEIRMRPKTTVENLRPGMSVLFPMEQIQ"
     gene            complement(14894..>15814)
                     /locus_tag="PLT31_02235"
     CDS             complement(14894..>15814)
                     /locus_tag="PLT31_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPW93984.1"
                     /translation="AEFDMAQNTILMDVATKYFTLRLTEELIVLRETTKKNLEEHLDR
                     SKKLEEGGQISKAERLRAEVALAEAEDALSDAKRDESLARMVLASLLQTDTNLVAVTP
                     VDVPVQMRSKDEYKQLALEKHPGLRQLQTERKRSKNAISAAQADYFPTVALFGYKELY
                     TKDLTILEPEWAIGAKVQMDLFKGGDTRAKVNNAKSLDRSLASLEKQTMDNISLLAEK
                     KWRELEHAQSRLESLNKTKELAEESLRSQKMAYESGLATSLDVVDAELALLRLKMADL
                     KAHYDAVIAWLGLLEISGEVSDAGIILEKK"
BASE COUNT         4760 a         2890 c         3230 g         4934 t
ORIGIN      
        1 aagaggcgat tggatccacc atgcttttat ggtatggtgg tttttttatt tcactttcca
       61 ttagtatggc acttttaatc aaagtcactc agaaattacc gatcggtacg gcgtatgccg
      121 tgtggactgg aataggggcg gtaggtactg tattgatagg gattcttatt ttcaaggagc
      181 cagccacttg gacaagaatt tttttcttaa cgacactaat catttctata gtgggtttaa
      241 aagcggtctc gtgatttgca cttgattatt tttggttcgt taactctgct ctatttttct
      301 atattctgta gtatgaatag cgattctcta aaacaaaaga ttcaaggctt cttagctaaa
      361 attaaaggct taagccgtac tcaaaaagtg atcattcttt ttgtgatggt cttagtgcct
      421 gcaggtattg ctatttcagc atttttgatt ctttatttta agggtaaaaa gaaacggtga
      481 ttttttcatc tcgtttgcca aaggatcttt ccgaaagccc tttctttaag gctttagaag
      541 aagaaaagca aaagtctcct tacattgatt tgactgtgag ctctcctctt aaagcgggca
      601 ttttatccga tatttcattt gagtatattg aaaaaaaatg gacttcaaaa gaagtctccg
      661 cagaaggctc tttgcttgta cgccaagcga tatgtgacta ttatgcttct cgtttcggcg
      721 tatttagccc tgaacaagtg attatctctt ctggaactag cgaagcctac tcttttttat
      781 tcaaaacgtt ttgtgatcct ggagatgtga ttttaacgcc catgccaggg tacccgcttt
      841 tagatacgct cgctaacctt gagtatttat cttgttaccc atacttctta gattataatg
      901 aagaatcttt aagcttttct cttgattctc aaacgttact ctctgctcct gaacgcgcta
      961 aaatattgct gcttgtttct ccgcataacc ccacgggcca tgtggtttct gagttagaat
     1021 ggaaacagat aatttctttt tgtgaagaac gttctttagt attagttgtt gatgaggtct
     1081 ttggagatta ttgttatgat ggtaaaccaa gatcttggtc ttatgactgc ggcaccgtgc
     1141 ctgtattttg gttaaatggt ttaagtaaaa ccgttgggaa cccgcactta aaactttctt
     1201 ggatggtgtt taaggcccca gaagatcagt ttgaaaaaat acgagaagct ctcacctttg
     1261 ttgcagacgc ttatttgagt gtatcgtctg gggcgcaggc cgctgcttta gatttgctcc
     1321 caaaggcagg ctctattcaa gaacgagttg tatcgcgcct gaaacaaaac ttggatgctc
     1381 ttaaggcgat gttcccgcat gccccaatag tacaaggggg ctggtatgcc tctatttgtt
     1441 gtgacgctta tgatgatgaa tctttggtgc ttgagatttt gaaagagaat cggttattag
     1501 tacaaccagg gttctttttt gatttttcag aagacgggtg gcttgtgata agcttgcttt
     1561 cttctcctga agattttctt tttgggctaa aaactattgc cccttttgtg aatcaaaacc
     1621 aataaaaagc gtgttttgaa atcccttatt aattctttta aatgcgaata aacgtttttg
     1681 atgtttcaaa agcggaatat ttctcgacat aattttcgag tgtaataaaa tccaataaaa
     1741 atcgtgtttt tgaaatccct tattaatttt ttttaatgtg aataaaaata tttgatgctt
     1801 caaaagcgga attttttcaa taaaactttc gcatgtaatc aaaaccaata aaaatcgtgt
     1861 tttgagatcc cttattaatt ctttttaatg tgaataaaaa tatttgatga ttcaaaagta
     1921 gaattttttc gatgaaattc tcgcatgtaa tcaaaaccaa taaaaagcgt gttttgaaac
     1981 cccttattaa tttttgattt gttttctcct tgtatcatga aatcggtttg tatgcgtttt
     2041 ttttattttg tttcataaat cctcttttat tcgctttctt tattggattt cattaattgg
     2101 tttttaaccg taattttcgg caaaatcgag ttctggaaaa cgtaaacgtt tcctaaggaa
     2161 atttgattaa cggttgcctc cagtatttgc atgaagggag tgtgtctttg ttgtccatat
     2221 tttgaatttt tcgggaagaa tcgccaaatt agggaatttg gatacattgg actcattggt
     2281 ttcatacaat attttcctta gttatcgagt tacatgggag cacttaggct tgatattttg
     2341 aatgatttga tgctttttaa taataataaa gtgtttttgt atcatgaaaa aatggtgttt
     2401 tcgttgaaaa atgccaaaaa ctgctaaaat tttataaaaa atgtgatttt tgtatcatgg
     2461 gttattgctt ttttgttttt aaagattatt tttcttaata tgaactcagt catcttatac
     2521 gaaaactatc gtgcttatat tcaggattat tattctgaac accacgaacg tttcggattc
     2581 acatggcgtc gttttgcaaa agcagcgggt tattcctctc ctgttttttt aaaactcgtc
     2641 agtgaaggga aatcgaattt aagtgatttg ggtattgagc gtacggctca ggctatgggt
     2701 ttggttggta acgatctttt gtatttccga gtattagttc attttaatca agaaaaaaaa
     2761 gcttcggtta aaaagaacta ctacaatgaa atgcgagcca tcgcgagtaa gtctcaggtg
     2821 tccttggtgg gggaagatca atacgattat ttttcttcct ggagaaatcc tgtgcttcgc
     2881 gaactcgcag actctttaaa gggtttaagc ccttcggact atgcttcgct ttttgtagaa
     2941 aaaacaactc ctgaagaagt taaaaaagct ttgaaaatac ttcttaaaac agggttaatg
     3001 actcagcctt ctccaaaaga atatgagaaa acagaggctg cactttctac aggtaattta
     3061 gaggtggctt ctcttacgat tcgtgatatg catcgtcaaa tgggagagct tgctgttaaa
     3121 tctttagatg atgtggatcc tcaagaaaga gatttttctg gtttaacgtt tggggtaaca
     3181 gaagaagctg tagaacgtat taaagcagaa attgccgatt tccgtcgtcg aattatgtct
     3241 atcgtcttag aagataaagg ttttgatcgg gtacttcgtt tgaatatgca gttgtttccg
     3301 ctcactaagc aagcaaaaaa agagagggaa aaacaatgaa aacttttaac gccatctttt
     3361 ggattatgtg cgtcgctggc gccttatttt gggcgtgtac tacgaacgtg gctggaggag
     3421 taacggaaga aacgaactct attgctggag tgatttataa ttcagaacaa aaggtggcaa
     3481 gcggggtaga agttttagct cgttctgtgg cagatactac caagctatat aaagatacca
     3541 cagacagcaa aggctccttt gcctttaagt ttaaggaaca agggctttat ggggtctctg
     3601 ctaaaacaag tgaacttgct tattatgaat tagtagaata taaaggggta accttaaatg
     3661 tagaggcttc tcttaataaa tacacttcat tcactggaaa attgacttta agtggtgctg
     3721 tttataatga acctattcaa gtctttattc ctggatctcc ttgggctgtt tatactaacg
     3781 agaatcttga atttgagata gattcagtac ctgaaggttc ttattatgtc tatgtgcgct
     3841 ctcctgatca gactcgcttt aatgatgctt atttctgggt ctctttatta ccagatacgt
     3901 taaaaggttc tcaagggcca ttgcctacct ctcttgcaac gagtgctcca tcggatgata
     3961 aaaatgctgt ttatgaatca gagaatagtg tatttaactt tagctggact attccaatgt
     4021 cttcagaata tgggcttgtg ggctggtggc ctatgaacta tatctctaaa gattcctctt
     4081 cgattcaata tattagtgat gcaagaggcc gtaccgatgt gggtgttgtt tatggaaagg
     4141 ccttcttaga ttctggcgtg ttagggcagt ctcttgtact acgtgggtca aatgattttg
     4201 ttgtcattga aagtgatcgt ggcgttttag atagtgcaac agcccttaca ttagaagctt
     4261 ggatttatat agaagactta aatgaaacag aagatgttac gaattatcgt aagaatttga
     4321 ttggaaaagt aggttttgga aactcagatg atcgtagtgt atttagtctt gcgattatta
     4381 agaatgaatg tgaagtagaa ggttctccag tacttgcttt ctttattgcg gacggctctg
     4441 gaacaagttt agattgtgca aatgctgtgg tggattctaa tgccataaaa gtgggcgagt
     4501 ggttatatac aacttccgtt tggaatgggc aaaccttgtc tttatatgta aacggtgctt
     4561 ttgtggcttc tagaggagtc tctgtggatg ttttgactcc aagtattgaa tcgctttact
     4621 ttggaaaaga aaacctgaat tttagattgg atgaagttcg ctggagtaca gtcgctatat
     4681 ctgcatccga tgttctatat agatattata atcaaggagg tgtccaatga tgacccaaga
     4741 cctacaagaa gaaatcgctt tttgcgaagt gaatgagcat ggccgtctat tgaatgccaa
     4801 taagcggttc tgtaaactat tcggctttgc tgaaaatgat gtcgcctggc attatgttaa
     4861 agatctttat cgtcatgaga aagattggga tgcctatcag aagtgttctc cagaacgtgg
     4921 ttctaatatt cgttttgtgg cacgcttaaa gaatagaaaa ggccgtagtt ttaaatgcag
     4981 tatttctaga gacgtttttc aagatgatac gggtcgcatt atttaccgca accggattca
     5041 aaaactttta gagtcttcta aagatacttc agataattat aatggatctt cttctaatga
     5101 atcttcattt gtctatttgg caaaatgtgt ttcttgtgca aagcaattaa aaataagtgc
     5161 ttcgcatcga gatagccgtt tgcctattta ctgccctgaa tgtgctgcga ttagtttttc
     5221 taaccatttt caatcaaaag aaatagcgct ttagaattat ttactcgccc ttctaaaaat
     5281 cctttgatta attacttaca ccgtcaattt ggcggtgttt ttttttgtct tattatacaa
     5341 caaaaagggg tttctttttg agataaaatg ggctcttttt gaaacaggag atgaagaaaa
     5401 atcaattaag agaaatgaaa gcaggaacga aaactggcga atctgagttg tatttggctc
     5461 tttgagtacg aactggcatg aagtcttaga atcgagttct gtttggcaag attattgcga
     5521 tagtaaaagc gtaataagac tattttctca aaagaggtgt taattatgtc tgatttttcg
     5581 aatgaaagtg aaaagatttt aggtaaagct caagaattga tggataagaa atctcattct
     5641 tatctaggtt ctatacattt ggcttgtgct cttgtggatt atccagatgc gcgtttgaaa
     5701 aatttataca aatcaaaaaa tgtcaaaatt aaagaactac gcactcgctt agattctatt
     5761 ctcgattctg tgcccaaagt tgctggcgtg aatccagagg cgagcccaga tagcgattta
     5821 aatcgtgttt tgagagccgc gattcaggcg gcaaggcaag tttcgagaac ggcttctcct
     5881 agcgatcttt ttgtggctct tcttcgtttt gcatctaatc gtcagttagt gaaaatattt
     5941 gaagatgcgg taggctctgc tgaagtggta gagacttggt tgtctgaccc aatgtgcgct
     6001 gctgcggtgg ctgatgaaca atctccatta aaactctatg gccgtgaact cgtggaactt
     6061 tgtgccgaag ggaagcttga tcctgtgatt gggcgtgaag acgaaattcg tcgcgtgatt
     6121 ttgattcttg cgagaaaaac aaaaaataac cctgtgttag tgggggatgc tggtgttggt
     6181 aaaacagcca ttgtcgaagg ccttgcagag cgaatctaca agggagatgt tcctgatgct
     6241 ttaaaaggga aaaaattatt tgctttagat ttgtctgcct tgattgcggg agcaaagttt
     6301 agaggtgaat ttgaagaacg gttaaaagcc gttttggaag ccttggaaga agatggcaat
     6361 acgcttttat ttatagatga acttcacatg attgtgggtg ctggtaaaac agaaggaagc
     6421 atggatttag gcaatatgtt aaagcctaaa ttagctcgtg gagaacttca ttgtattggt
     6481 gcaacgacca ctcaagaata tcgaaaatat attgaaaaag attcggcgtt agaacgtcgt
     6541 tttcaacctg tttctgtttt tgaaccttct gaagacgagg ctctttccat tttgcgtggc
     6601 attaaagagg gctttgaagc ccatcatggt gtgcgtttgc atgataatgc tcttgtttct
     6661 gcagtgaagc tttctattcg ctatatcgct gaccgtaatt tgccagataa agcgattgac
     6721 ttgattgatg aagcggcaag ctttgtgaaa acgcagctcg atacggttcc agaagcatta
     6781 gataatttac agcgtaaaga gctccagttg aaaattgagg aacaggcgct ttctaaagaa
     6841 acggatgata agagcgcgaa acgtttgagt gaacttagag aagagcttgc tttaacgagt
     6901 gcagcggttt ctgaaatgca agagcgttgg caagaacagc gcgctaaatt ctctgaatta
     6961 aaagaagcaa aatcttcttt gaaaaaagcg aaagaagaga tggaacaagc agaggctcac
     7021 tacgatctaa ataaagcggc agagcttaaa tacaatacga ttgtgggctt agaaaatcag
     7081 gtgaaggcgc ttgaagaaga ggcgcgtgct gcttcgcaga acggcgaact cacgcaagaa
     7141 gtgaatgagg aaacgatcgc actagtcgtg agtcgctgga cagggatacc tgtcacgcgc
     7201 ctccgtgaag gagaacgctc gaaattattg cttttagaag accgccttca cgaacgcgtg
     7261 attggccaag acgatgctgt taaagccgtt gcagaagcca ttcttcgtaa tcgtagcggt
     7321 ctttctagag aaaacgcacc tatcggcagc ttcttatttt taggcccaac aggagttggt
     7381 aaaacagaac tcgcaagagc tcttgcttat acgcttttcg atagtgaaga cgcgatgatt
     7441 cgggtggata tgagtgagta tatggaaaaa cactctgtcg ctcgcttaat tggagcgcct
     7501 ccaggctatg tgggttatga agaaggcggc cagcttaccg aagccgtgcg tactaaacca
     7561 tatagcgttg tacttttaga tgaagtcgaa aaagcgcatc cagacgtctt taatacgcta
     7621 ttacaagtgt tagatgatgg gcgattaacc gatggaaaag ggcgtgtcgt cagctttaag
     7681 aatacgattg tcttaatgac ctctaattta ggcagtcatt tgttccaaga agaacaccaa
     7741 aaggtttctt tagaacaagt catgcccgaa cttcgtcgct tttttaggcc agaattctta
     7801 aaccgtttag atgaaacgct tgtgtttact tcactttctc gagaccagtt gcattcgata
     7861 gtcaaactca aagtgaatgc gttgcaagaa cgtttatcaa agcaaggctt cttaatgagc
     7921 gttacggaag aggctgttgc agcgattgca gagaaatcct atgacccgca atttggagcc
     7981 cgtcctattc agcgttattt acaacgagaa ttagaacgag tgttgtctca cgcgatcatt
     8041 gcaggagaag tgaaaacaga aattcctttt gtagtagatt acaaagaaaa aacctttgta
     8101 atacaataag aaggaaccat ttgcggaaaa tcgtttagag agcgattaag gtataaagag
     8161 caatttgttt ttgagaattg ttcttttcta aaagaatacc gatatcgact tctaaacgaa
     8221 gccgtacatt ggcgattctc aattctaatt ttggcaaaag atcaatcata aaatcgtctt
     8281 gaatattccc tatgctacct gatgaattca tttcgaccat cgctaaaaag gtttttccaa
     8341 tatgaagagt aggcataaaa ccaacttgta atttcatata accatcagat cctgtaatac
     8401 catctaaaaa gccaacacgg atttcataaa ggttcgaaaa acggcgagct acgtgagttt
     8461 gctggctata ggtaaaaata aggccaccac cataatttct atttaaagca aaataggtgt
     8521 ctataccaac gtaagagttg taagacaagt tgtattttgc tcctaaatca atgtttccaa
     8581 taatatgttc cagggaattt ggggattcta gaaataattt accaccaatt tcaatttcgt
     8641 tattgaggcg agcctgaaaa ttaacaggaa aatttacatc agaatgaaac cccgtacgaa
     8701 ggcctattcc aaaccccatc ttatcataag tgcgaatttc tgggcctaat gtgaaacggg
     8761 cgtcccacat gcggtcaaga gcatgagaat aaagacttaa aaaagcaagt aaaaataaaa
     8821 gttttttgac catagggtta aatattaata aattattaag tcgtaggagg tttttatgat
     8881 tgaagcacgt ccggaactag ataaattatc tgattatgta ccaggaaaat ctatagatga
     8941 gattcgtact cgctatgggt taagtaaagt ggtcaaactc gcctctaatg aaaatccgtt
     9001 aggagcctct ccaaaagcaa tggaagccta tatgaaaata gcttcttctt tgcatttgta
     9061 ccctcgtgga gatgcccctc atttagtgga agcactcgct tctaaatatc gagtaaaaca
     9121 taatcaagtc atcattggta atggttctga cgaagtgatt gatatggtgg gtaaagcttt
     9181 tattcgccca ggagacacgt gcctttcttt agaacctact ttttctgttt atcgttctgt
     9241 gacgtattct tatagtggcc actttgcaag cgttaattct ctagatcaac aagaaccact
     9301 caaagaatta gccaaaaaaa tagatgagaa aacaagaatt atttttattt gtaaccctaa
     9361 taaccccacg ggcggttatt tttctgaaga tgaaatagtt cagtttatgg tgcaagtgcc
     9421 tcgtcatatt cttgtttttg tagatgaagc ttatgctgaa tttgcaacgg cagcaaatta
     9481 tcctagtctc atcaaaatga ttgatctttt tccgaattta ctcttaaatc gcacgttcag
     9541 taaaatttat ggcctcgctg ggttacgtgt aggctatgcc atttcgagcc ctcaagtgat
     9601 ttctaaccta tggaaagtaa aacctccatt tgatgtgaat ttggcggcac aagaagctgc
     9661 tcttgccgct ttacaagata cttcccatat taagaaaacc ttagaagtca ccgcgcaagg
     9721 aatcgagtat ttaacaaaag cactttctac tatggggtac cgtgtattac ctacccaagc
     9781 gaatttcatt tgcgttcata taggtgcaga tgctaaaaag atggtttcct ttttagaaga
     9841 aaagggaatg attatcagag gcctttcgag ctttggaatg ccagaatggg ttcgtattac
     9901 aataggtaaa cacgaagaaa atcaattcct tttagaatgt gttaatttat ggagaaataa
     9961 tggcagagat taaagagctc cttgagatgg cgttaaaagc ctcttctctt gcagaagaac
    10021 agattcttaa atattatggt tcttcttatg aagtctcttg gaaagaggac caaactcctg
    10081 tcaccatcgc tgatcaaaaa gccgaagaag tcatgcgttc ttttttagaa aaagaaatga
    10141 aagacgtcgg ctttattggc gaagaatttg gtgttgaaaa cccaaaagcc tcgtaccaat
    10201 ggattatgga tcctattgat ggcacaaaag cttttatcca tggcgttcct ttatttggca
    10261 ctctccttgc tctttataaa aatggtaaac cttgtgtagg cttaattcaa ttgcctgcat
    10321 taaaaacatg tttatgggct tctaaagggg cgggcgctta tgtagacggg catcaagtcc
    10381 attgttcaaa agtatcttct atagaagaat cgctcgtact ttctggcacg atcaacacca
    10441 tggaagataa gggattgggt gagtctttta aaacacttcg ccgtgcttca aaattatatc
    10501 gtggttgggg cgattgttat ggctattatc tagtggcgag tggccgagct gaaatcatgg
    10561 tggattccgt tgtctctctt tgggatattg ctcccatgcc tgtgatcttt agtgaagcgg
    10621 gcggcgtgtt ttctacgatt accggagaaa catctttatt caacaatcaa ggagaaccaa
    10681 tccactccat atacgaaggc tatacaggtc ttggttgcgc tccctccgtc tattccaaag
    10741 ctcaagaaat actttcagaa taaatttttg aaaggaatgg caacgtttaa aaaccttgtc
    10801 gttttctaat tcaggctctt tttgattggt aaaaaagctc ttttttgttg ttttttgaac
    10861 atcacaaaaa acatggtata ccccttaaac atttgtcttg ggttatttag ctcattttaa
    10921 gctttttttt aataaaaaaa taaacagagt atagttgtgg ttaaagtgat cctataatag
    10981 tactttataa agagtcaacc acacttaaaa aaatatagga gcgcaggaat gaaagacaac
    11041 aaatacagat ctatagtgaa attagatttt caatatgcac atcgtttcat gaattatcca
    11101 ggtgaagcaa gctatttaca tgggcattct ggtttactca cacttgaaat tgaaggcgaa
    11161 ttagacaagc acaattatgc ttatccagtc aaagaagcac aaaaactagc atgggccgtg
    11221 gccgacaact ttatgcatgc caccattttc caagaaggcg atcctctttt tgatctcgtt
    11281 ctacaaggct atgagaaaac agggatgaag ggtaccacca atcgatttgg tacacctgcc
    11341 aaagtactcg atcacgaatt agtcaaatcg ttccctgaaa gcaggcttgt gctcagcaaa
    11401 ggcccttcta catgcgaatg gttctgtact gtattttatg aactcttaaa agacaagttg
    11461 aatatcaaaa aagtcgagtt ccgctcttct tcattgaatt gcgcttctaa agaattctaa
    11521 ttgttaaaat cttatttgta atgaatgctc acttatttta agtgggcgtt ttttatgaaa
    11581 taataggtta catatcccca gttttcaatt tggatttgtc tcttaagaaa gaaagcaata
    11641 agccaagccc taaataaata acgctttgaa ctcccatttt tactagaaac acggaggcgt
    11701 cttgaatacc cgcgcccatt tgttgaatcg atagccaagc tggcaccgca taagaacttg
    11761 gtaaaatagc agaaagtgct gccatccaat ctggaattaa gtagcgtggc caactgaaat
    11821 tagaaaggaa caaaataggg atcgagagaa acaagaagat ttgcatgcct gtttctcgac
    11881 gtagaaacac ttgcgataaa accattccta agttgatcga agccgaaaga aatacaacag
    11941 caaagacaat catcggtaat ggttccccgc gacgaggaaa gtcaaatacg ttgtagacca
    12001 caaaatgata aaaagcgata aagcaggcgt aatgtaaaaa gtacgctaaa gagcgaccaa
    12061 aatagcggta gggtaaggat tcgttttcgg cagcactttc ttgttttttc tttcgaaatt
    12121 ttcggtgagc cctcgctccg cctaaagtac agattccgat tattaatgat tgttgtaaaa
    12181 taagaactaa aacgcttggc accacataat tagaataact ccctgttctg ttatacaatg
    12241 tctggatttg aataggaaag ggatctctca tggccatcgc tctttttgca ggcacttgtt
    12301 tatcaagagc aatacgtttt acttttgtct ctgcacctaa ggtaaggcca cttgttgcaa
    12361 aagcagtacc tatgtttcca tgaagcatta cgtaagcacc atgcgtaaag acatttaatg
    12421 tcactttttg gcctgtacgt atatcgcgct ccatattatt tgggataatc atatagccat
    12481 aaatattccc atcagccatg gataatttag cttgatgaat atcatcaaat acatatttca
    12541 catcaatctg ttgcgccgct ctagacattt ggattaattg cctagacatg gctgttttat
    12601 ctaaatccac aattccaacg ggagcttctg tgactacttg ttttaaataa ggggtagggt
    12661 agtaaaaagc ataaataaaa ccagcaatta caagcaataa tatagccgct ggatctgtga
    12721 aaaacagttt ccattctgaa aaagtcactt ttaaaaaacg cttaatcatg gttccatttt
    12781 ttccaacgca taaacatgaa gcgaccacct aataaatgcc aaaataaacc catgccgagc
    12841 aaaatgacaa gtgagtccca agaggggata agccctacat cacctaagat catttgagac
    12901 tgtattttta agaggtgagt taaaggcaaa aggaaggcaa agcatcgaac aaaaaaaggc
    12961 atggcgataa tggggaacgt ttgcccagca aaagcaaaag cagggcctcc aataacgcct
    13021 gcgacacttg ttgccatacg catgtttcca gtaatcccta caaaagaaat tccagcccca
    13081 gtacaagcaa gcaccatagc caattgccca gcaccaacca ttataaactc atttaaagta
    13141 agtggtgcta agaatcgatg agtgtatgta tagatggcta gcattaaaaa ccaagaaatt
    13201 aaaattaaag gaactaccga ggcaaaccat aaaatcattc tggatccacc agcagcatca
    13261 atatattcct ttgttttcct atctcgaaga gggaacgaaa aaacatatac agctataagt
    13321 atggaactca tgtgaaatat ggacgttact aaaccgagcc ctaaataacc ttgataatta
    13381 ccagtgctat tcccaacaga atggatctct gttttgatcg gttctttgat gtggcgagta
    13441 aataaattag atcctatagt agaaaaaaca gaacgtattt ctttaagagc aaacatattg
    13501 gttagatagt attggccatt agaatagatt ggaactacag gggtttcata acgcaaaact
    13561 cgtttttcaa attggtatgg aattacaata aacgcctgta tctcaccacg cctgatgccg
    13621 cgttcgcatt cactaaaatc agaacaatgt aattttaaat ctaaaactgg atttgctgat
    13681 aaactgtatt ctaaacgttc cgaaagcttt gaataatcat aatgaataat acctatggga
    13741 atccgttgta ttgattcagc agaaaaaaag tgaaccataa agatagaagt gctaacagga
    13801 agaacaagta aaatcatcca cagtacaatg ttattgcaga ggtatatttt tttgactgcc
    13861 ctaaaaaaat cttttagcaa ctggatctcg attgattaaa gtggttactg aatttgttcc
    13921 ataggaaaaa gaacgctcat tcctgggcga agattttcca ccgtagtttt tggacgcatc
    13981 ctaatttcaa aagactttag atcataaccg ccgctttctt tggagctacg ccaagttgca
    14041 taatctccaa cagaagcaat gtaggtgact accatttcga tcgttttatc taaagcgggg
    14101 atttgaaccg taaatgtttt ccctttagaa atatgtttta agaaatcctc tctaatatga
    14161 aaaacagccc aagaatcatt tagatcagtg attgcaatga cgggcattcc tgctcccacg
    14221 atttcacctt cttcggcgag ctttaacgaa acttcaccag aaatcggcgc acgaatctta
    14281 gtttcatcta aatacgcatc gacttctgca tttgctccac gagcttgaat tacaagcgca
    14341 ttcgcagcgg ctttatcttc tacgcgtgcc cctgcgacag cttgttcgta ttgtgctttc
    14401 gcagcggttg ctgctgccac cgccgttttc atctgagttt ctgcttcatc tcttttttga
    14461 agaggaatca caccatcgtt atataatttt tgaactcgat caaaagtggt tttagcaaga
    14521 tcagcggcat cttgagcacg ttctttcatt gcttttaatg ctgtgatttc ttctgcacga
    14581 gcgcctgtat tggctttatt ggattgagct tttgcagcac ctaaagcccc ttcggcctgt
    14641 aatttttttg cttctaactc tctactagag ataatggcga ctaaagaatc tttaacaacc
    14701 atatcgccct cgtgaacaaa taagcgttct aaacgcccag gaattttacc tgccacaaga
    14761 actcgacggg cttccatttg accttgcaaa aattcttcac gaggctctgt cgcaaatttc
    14821 tgaagagtgg taataccagc aatgatcaaa aaaatcaagg ttaagataag aagaacttta
    14881 agtactattt tcattacttt ttctccaata tgattccagc atcagacact tcgccagaaa
    14941 tttcaagtaa accaagccaa gcgatcacag catcgtaatg agcctttaag tctgccattt
    15001 ttaaacgtaa aagagctaat tcagcatcca ccacatcgag gcttgtcgct aatccagatt
    15061 cataagccat tttttgacta cgcaaggatt cttcggctaa ttctttcgtt ttatttaaac
    15121 tctctaaacg actttgagca tgttccaatt ctctccattt tttttccgct aacagagaaa
    15181 tgttatccat cgtttgcttt tctaaacttg ccagagaacg atctaaagat ttcgcgttat
    15241 tcacttttgc acgagtgtct ccgcccttaa ataaatccat ttggaccttt gctcctatgg
    15301 cccattctgg ttctaaaata gttaaatctt ttgtgtacaa ctctttgtag ccaaataacg
    15361 ccaccgttgg aaaataatca gcctgagccg cactaattgc gttcttactg cgtttccgtt
    15421 ctgtttgtaa ctgacgaaga ccaggatgct tttctaaagc caattgctta tattcatctt
    15481 tagaacgcat ttgaacagga acatctacag gagtgaccgc tactaaatta gtatctgttt
    15541 gaagcaaact agcaagaacc atacgagcga gcgattcatc acgtttggca tcacttaggg
    15601 catcttctgc ctctgccaag gccacttcgg cacgtaaacg ttctgctttg gagatttgcc
    15661 caccttcttc taatttttta gaacgatcta aatgctcttc taaatttttc tttgtggttt
    15721 ctcttaaaac aattaattct tcagttaaac gaagagtaaa atattttgtg gcgacatcca
    15781 ttaaaatggt gttttgtgcc atatcaaatt cggc
//
LOCUS       DAOOBB010000025        15246 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_5070_length_15246_cov_5.390034, whole genome shotgun sequence.
ACCESSION   DAOOBB010000025 DAOOBB010000000
VERSION     DAOOBB010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 15246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15246
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5070_length_15246_cov_5.390034"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="PLT31_02240"
     CDS             complement(<1..964)
                     /locus_tag="PLT31_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HPW93985.1"
                     /translation="MQTLEGFEYTPFIPKSLLSIAEKIESAGGTSYLVGGWVRDALLA
                     EECRDFDIEVYGLDQDVLLKILSQYGRPNLVGRAFGVIHLAHKGFSLDFSFPRTESKV
                     GEGHRGFLVQTHTHLSFREAAYRRDFTINAMGMKLPDLVLEDPYGGRDDLEKRILRHV
                     SPAFVEDSLRVLRGVQFASRFHLTLAPETISLCRTLSIEDLSYERIFEEFKKWLLKPG
                     KPSLGLQAFLDMDLNRFFPEIKPLNGSWELLGSFLDAIHESFVSFSATDQLILAFTAL
                     LSENKEEQAISRFLERITNEIKILRGVPLLWREVSLWKPERENLA"
     gene            complement(951..1220)
                     /locus_tag="PLT31_02245"
     CDS             complement(951..1220)
                     /locus_tag="PLT31_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93986.1"
                     /translation="MKKYCWFLLSLFLISCASQRQKAVCSEIEYRLENLGHSPDQRAF
                     VEEELRQCQEQQKEYAKSDVLTNKAKKSIYDLYQESKGDSLNANP"
     gene            complement(1296..1664)
                     /locus_tag="PLT31_02250"
     CDS             complement(1296..1664)
                     /locus_tag="PLT31_02250"
                     /inference="COORDINATES: protein motif:HMM:NF015004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HPW93987.1"
                     /translation="MTRINVGIKPSELIGKMLVAEHREIKRIPNIIKTGKYSMQGQPK
                     LFTLGKGHVKFFYDKLEFLRKRYELLYAECKKRGYQVTYFGDSFTNLPKELMNDYKPK
                     KRDRELVLLRIAERTSQIEK"
     gene            complement(1682..3862)
                     /locus_tag="PLT31_02255"
     CDS             complement(1682..3862)
                     /locus_tag="PLT31_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93988.1"
                     /translation="MSYGSKAIEGFSVPSEIHSATIYQNHLFLATDGGIRFYLGDNQS
                     VVYTADDGLEATMFYAIASNENALYAVSAKGLIAKKTGLTSNFKVINRSFVSGNSQVL
                     KELILLKDSVMVLAFNDKLAFIDLISERSLISLTSIASHRLNTEMITAMTIKEDSLYV
                     AINNNVYARKMNWSDMGADLFLADPSSWKKYSITLPPDTIRAMAWLKDSLVVRAERGS
                     FYYNEKGEKTEASLNQASITLEDKELNDSLLFKNDSSLVEWILKTENGHYFIGPSTVI
                     YREGSKMADVYAWNFYSLGPAHQTITLNTGGVLAASPYGDISLSDGFTWTPKQRLNFT
                     PFTFEKEAITSKMKSISIDKNGNLLYGLWGGGFILYKNLGLELKKYIVPGTTCIDEFI
                     DDYTVTIGSTTAPDQSGFFVSYWFSPNYGFAYIDLDGEVSCANRVGSTSYPGPLQVIA
                     SAEDPDQWLLYSSAGNVNDVNGSGFLDVFSFTKPSKTGGRIVDVEHRTYDGPNKEYIV
                     DMDFDPSGRLWVAGHTSLGYLDPEDDQIRHPHKISGFSGASNSSMEVDVQGNIWLGSK
                     NGAYRFSPKNGSPDTLTTLHWATKQGLVSDVIYDIAIDSIQGMVWFGHDLGVTRYTRK
                     DLRKADAFMTEDSPKKTIAYPNPFKPKVHGKMIFDYVAEEARIQIFNAGGALVRSFAK
                     EDLLGGRLEWDGTDLKDRLVAPGLYHYLVKKGSKLEKGKLLIIH"
     gene            complement(3978..4505)
                     /locus_tag="PLT31_02260"
     CDS             complement(3978..4505)
                     /locus_tag="PLT31_02260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPW93989.1"
                     /translation="MFKNIEFSVKKKQEAFLMSIWNKNSQRIYQNCLKICNDQETAKD
                     LQQEVFIKIFKHVHVFKDYDNKDAWIQFVCRNHCLDFLRRQKARPEFIANENMGSYEV
                     VGDDFKSEQALFYKLEQVLMTLNILDRMILELYFIGGMNGDDISKCLGLSKTSVSLKI
                     KKALNKANFFFNNDG"
     gene            4654..6381
                     /locus_tag="PLT31_02265"
     CDS             4654..6381
                     /locus_tag="PLT31_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPW93990.1"
                     /translation="MSIFSFAQDTSMEIDTSTMPPPGIELSYPNKSLAVGDCVIVEIV
                     IPEKWHVNANVVSDEFLKASQIEIAAKGIVFNQPIWPTYKKKYNDALEMDIFVFDGKI
                     QIKIPIKELEASYDSTTTNTTFHYQACSNFICLAPNQVHFSLSPSAATPIPLKKNDSS
                     ILVLLFFAFIGGFILNLMPCVLPVLSLKLFSLIKQSGKSRKRLFTLGLSMTGGILFSF
                     WILATIIISLKSAGSLVGWGIQFQSIGFIAFMVILLSAFAMNFFGFFEIWLPGKTLTK
                     MDGFTKKEGLAGAFFTGSLLVLLSTPCSAPFLGSAMGFAFSASAPVLFLFFTVAGLGL
                     SLPYLIITLFPVLLSIFPKPGPWMVSLQKWMGLLLLGTVLWLLWVAKQTVGLEGFFLL
                     IILSLMSMGFSFLTGKIAPPYKPFYREILLLLSSVLILFFTWQFAVSPTIDKALKEKK
                     AAAFSLEKDESGFFPYSKEHLTSALEAGQPVLLDITADWCLTCKANEAAVLSQESLGY
                     VLDKHNVVKIKADWTLQDKEISELLKSLGRSGVPVYAIYLPHNPQNPILLPEILTTNS
                     ILDVLDSNQ"
     gene            6396..7631
                     /gene="glgA"
                     /locus_tag="PLT31_02270"
     CDS             6396..7631
                     /gene="glgA"
                     /locus_tag="PLT31_02270"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HPW93991.1"
                     /translation="MKAALFTNEFPPEIYGGAGIHIQYLSKELRALCQIEARCFGPQN
                     DEEDNLRAIGFSEKINNAPLSDIRVKKILKPLDINIQWAASLKDIDIIHCHTWYSHFG
                     GVLSSRLLQCPLILTTHSLEPHRPWKSEALGQGGYDMSCWIERTAYEAADGVIAVSQG
                     MKRDVMDLYGVPEDRVQVIYNGIDPDFYKPTFDNAVLKKWNIDPSRPFVLFVGRITRQ
                     KGISQLIQAIPQIDPNAQIVLCAGAPDTQELADECKNLILEVQKTRDGVIWIQEMMPH
                     SELRVLYSHATVFATPSLYEPFGIINLEAMSCGTPVVGSAVGGIPEIIVDGETGFLVP
                     LKAKSTINFDPIDPKEFQMQFADKLNKILSNPALAQKMGVVSRQRAINVFSWKSIAKQ
                     TFDFYSSCIERYKKEGRRV"
     gene            complement(7639..8205)
                     /gene="folE"
                     /locus_tag="PLT31_02275"
     CDS             complement(7639..8205)
                     /gene="folE"
                     /locus_tag="PLT31_02275"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HPW93992.1"
                     /translation="MDLKKMEEGFRLILEGMGENIHREGLIDTPKRVAKMYAELMTGL
                     SGELKEEEILKTQFHEKYDEMVYLKDIEFASMCEHHFLPFTGRAHIAYIPNDRIVGLS
                     KLARAVEYYARFPQVQERMTCQIATLLQRELNPKGVAVVIEASHTCMTIRGIKKAGAV
                     MVTTQLLGRFKSDEKTRAEFMATIHAAH"
     gene            complement(8208..9563)
                     /gene="argA"
                     /locus_tag="PLT31_02280"
     CDS             complement(8208..9563)
                     /gene="argA"
                     /locus_tag="PLT31_02280"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HPW93993.1"
                     /translation="MTVIPDFKEQKIEVASFIREVFSYITRFKGQLFVLKIEDSLMNH
                     PLFPVLIKDISFLHKAGIKVLIVPGTRNSIDAQLKAWDIDFNFNNGIRLTSEEALPLI
                     EQASLGVAQRIMSHLTAGGCDGIQGNWVSARSLGIIDGVDYMRTGKIERIQRNIIERL
                     LAENFIPIIPPIGWNKLGHAYNISSTELATELCKYLQVAKLFFIGTENGISMDGLKTS
                     SLTQYLEVTDSGVISALDIDQAKEILDLNPQTLNFAQKDYLLNAIQACKVGANRVHLI
                     SGEIQGSVLQEVFSSRGDGTMVYSSQYSSIRPAVIDDIADILRIMQDYIQKGFLISRT
                     QEAILEKLNDYVVYVIDNAILGCGALHPYENDSAEIAAVAVAANYRKSGVGDAVVRHL
                     LSAAKIRGYKKIFLLTTQALDWFYQFGFEDGTIDDLPPSKRKNYNTRRNSRVLVYSLE
                     K"
     gene            complement(9627..10664)
                     /locus_tag="PLT31_02285"
     CDS             complement(9627..10664)
                     /locus_tag="PLT31_02285"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPW93994.1"
                     /translation="MKRFGLFITACALTSLTACSSAPKPTTEQVSKPDTSAVASIKDS
                     LKTQVERYSYALGMDVGKTLKSILVDVNMDAFNLAMTHQMSGKASLLTDEEAETALET
                     LLLQVQEKRDQTAKEEAQKEKIRQTEFLAKNKLDSTVIVTASGLQYKIIKDGQGLSPK
                     RTEKVKVHYIGSLLDGKEFDNSIKRGEPLTFDVAHVIEGWQELLTLMKPGMKVKAWIP
                     SALGYGEEGAEPIIPRNALLVFEVELLEVFAEPSPQEVIAPVDSLAKPEVKTEAQPAA
                     KAEAKPEVKTEAKPAAKAEAKPEVKTEAKPAAKAEAKPEVKTEAKPAAKAEAKPAAKA
                     EAKPAAKTDKK"
     gene            11012..11263
                     /locus_tag="PLT31_02290"
     CDS             11012..11263
                     /locus_tag="PLT31_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93995.1"
                     /translation="MSQKFNQIINDLESRIDLFNQNKLSLSLKLNHAVQEDSEELITN
                     KTRSHKTPSFEKTGWIPGALSYPWIISFLFIPEILKNLV"
     gene            11302..12150
                     /gene="folD"
                     /locus_tag="PLT31_02295"
     CDS             11302..12150
                     /gene="folD"
                     /locus_tag="PLT31_02295"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HPW93996.1"
                     /translation="MPALILDGKSLAKQTENQLKIRVDNLRESSGKTPVLATILVGND
                     PASETYVKMKGNACQRVGMESLRVFLPKNINTEELLDKIRELNDNPNVHGILLQHPVP
                     RHIDERKAFETIDLRKDVDGVTCLGFGRMSMGQEAYGSATPAGIISLLKHYEIPLAGK
                     HAVVVGRSAILGKPMAMMLLAENCTVTICHSKTNNLPDLIKQADILIGAVGKPEFIKT
                     SWIKKGAIVVDAGYHPGGVGDIEKEGLDSIASAYTPVPGGVGPMTINTLIRQTVESGE
                     KIIKPI"
     gene            12502..13893
                     /locus_tag="PLT31_02300"
     CDS             12502..13893
                     /locus_tag="PLT31_02300"
                     /inference="COORDINATES: protein motif:HMM:NF014113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW93997.1"
                     /translation="MKKNILAFFASIFLLLVIWNCTESPNEPKQTTDNVGATFYPVLT
                     SGGFLILPNNLVTDFSGSTVVGVYNSDGTITSSEGIVLESNVNKNALSAITPALFVIN
                     PDGSVTDINGTPIGVMDPSGNIVLPNGAVYSGSGDLLIPPTILSSATEPLSSAFIPLS
                     NGEVPLSSAFIPFSSGEVPYSSAVIIPTSSSSQFVTPNSSSNWVPPTSSWTAPSSSSV
                     PKSSSSVAKSSSSQGSQAPNIKVIQGGKSGSGYATRYWDSCKPHCAWEGKGGPVARTC
                     QRDGTTRAGSGESSVCDGGNAGTCFDQTPIIINNDYAYAFAATPGGGNDCGKCYMLTF
                     KGTGADATNTPTDDAHRSIKGKKLIVMSNNIGYDVNHGQFDIMIPGGGPGAFNGCQQM
                     GISCAGAQYGGFLTTCNYKKDCLINMCNQEYGNKPSLKEGCLFLANWMNAANNPEVDY
                     VEVECPSELTSKY"
     gene            complement(13959..14756)
                     /locus_tag="PLT31_02305"
     CDS             complement(13959..14756)
                     /locus_tag="PLT31_02305"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014544918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HPW93998.1"
                     /translation="MNQKEFLDDALSIAETLARTAGNSALDLQSRLGQVDYKTPKDVV
                     TKADYLSEKIIVEGLKNAFPTHYIRTEEAGIIQGEDPNYLWIIDPIDGTINYSRGIPF
                     WGISIALHFNKKPLLSVVYLPRLEEMFTAILDRGAFLNGKPIHVSDVTNASQAIVSNG
                     DFNVGHIDKINKHNLQNFEIQANHFQRVKCLGSAVVEGCFVASGRLDLFVMTMSYPWD
                     IAAIALIIKEAGGEATRIDGAPLAFIDAEQVVFSNRLLHQKWLDITK"
     gene            complement(14848..>15246)
                     /locus_tag="PLT31_02310"
     CDS             complement(14848..>15246)
                     /locus_tag="PLT31_02310"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HPW93999.1"
                     /translation="KGITYVSNTQAGAVGQKTKYPIQVLRTKQANCIEGALLFASILE
                     SIGLDVVLVSIPGHAFVGWKTEEEGNTYNYLETTLTWNEMPVSFSTAVNSAIETYNEE
                     VTNGNFESGESRIISVAQAREYGIKPNDIP"
BASE COUNT         4752 a         2984 c         2555 g         4955 t
ORIGIN      
        1 ccgcaagatt ttctcgctct ggtttccata acgatacttc tctccatagt agtggaacgc
       61 ctcgaagaat ttttatttca ttagtaattc gttctaaaaa acgactaatg gcctgttctt
      121 ctttattttc agagagtaag gctgtaaaag caagaatcaa ttgatctgtg gctgagaaag
      181 atacgaatga ttcgtgaatg gcatctaaga aagaacccaa taactcccaa gaaccattca
      241 aaggtttaat ttcagggaaa aaacgattta gatccatatc taaaaatgct tgcagaccta
      301 aagagggctt tcctggtttc aataaccatt tcttaaactc ttcgaaaatc cgttcataag
      361 agagatcttc tatagaaagc gttcgacaaa gagaaatggt ttcaggagca agagttaaat
      421 ggaatcgaga ggcgaattgg acacctcgta aaacacgtag cgaatcttct acaaaagcag
      481 gactgacgtg acgtaagatt cttttttcta aatcgtctcg gccaccatag gggtcttcaa
      541 gaactagatc tggaagtttc attcccatcg cgttgatggt gaaatctcga cgataagcgg
      601 cttctcgaaa actcaaatgg gtatgcgttt gaactaaaaa acctcgatgt ccttctccta
      661 ctttactttc tgtacgtggg aaagaaaaat ctagagaaaa tcctttatga gccaaatgaa
      721 tcacaccaaa agctcggccc actaaatttg gccgcccata ttgagaaaga atttttaata
      781 aaacatcttg atctaagcca tagacctcta tatcaaaatc gcgacattct tcagcaagaa
      841 gagcgtctcg cacccatcct cccactaaat aagacgtacc gccagcactc tctatttttt
      901 cagcaataga taaaagtgac tttggaataa aaggagtgta ttcaaaacct tcaagggttt
      961 gcatttaagg aatccccttt agattcttga tataaatcat aaatactttt ttttgcctta
     1021 tttgttaaaa catcggattt cgcatattct ttttgctgtt cttgacattg ccgtaattct
     1081 tcttctacaa acgctcgttg atcaggggaa tgtccaaggt tttctaaacg atattctatt
     1141 tctgagcata ccgctttttg acgctgcgaa gcgcatgaaa ttaaaaataa gcttaataaa
     1201 aaccaacaat attttttcat tcttcaattg gaaaataact tttaaaaatt ctttaatcta
     1261 gagaagagac tagaatcact aaaaaaagta gttttttatt tttcgatttg gcttgttctt
     1321 tcagcaattc ttaataaaac aagttctcta tctcgctttt ttggtttata atcattcata
     1381 agttcttttg gtaaattcgt aaatgaatct ccaaaatagg ttacttgata acctcttttc
     1441 ttacattctg cataaagcaa ttcataccgt tttctgagaa attcaagctt atcataaaaa
     1501 aacttaacat gcccttttcc gagagtaaac agcttaggct ggccttgcat agagtatttg
     1561 cctgttttta tgatgtttgg aattcgttta atctcacgat gttctgcaac aagcattttc
     1621 ccaattaatt cggaaggctt gatgccgaca ttgattcttg tcataaaacc ccatcaaaaa
     1681 attagtgaat aatcaaaagt tttccttttt ccaattttga accttttttt accaaataat
     1741 gataaagtcc aggagccact aatcggtctt tgagatccgt tccatcccac tctaatcggc
     1801 cgcctaacaa atcttctttt gcaaaagaac gaactaaagc acctccagca ttgaatattt
     1861 gaattcgagc ttcttctgct acataatcaa aaatcatctt accatgtact tttggcttaa
     1921 atgggttagg ataagctatt gtttttttag gtgaatcttc agtcataaaa gcatctgctt
     1981 tacgtagatc ttttcttgta taacgagtaa ctcctaaatc atggccaaac cataccatgc
     2041 cctgaataga atctatcgct atatcataaa tcacatcact cacaagtcct tgttttgttg
     2101 cccagtgaag agtggtcaaa gtatcaggag atccattttt tggggaaaat cgataggcac
     2161 cattctttga tccaagccat atgtttcctt gaacatccac ctccatagaa gaattcgagg
     2221 cgccagaaaa accactaatt ttatgaggat gcctaatttg gtcatcttca ggatccaaat
     2281 aaccaagaga agtatgtcct gccacccaaa ggcgtccaga aggatcaaaa tccatatcta
     2341 caatgtattc tttatttgga ccatcataag ttctatgttc cacatccaca atacgtcctc
     2401 ctgtttttga tggctttgta aaggagaaaa catcaagaaa accagatcca tttacatcat
     2461 ttacattacc tgcacttgag tatagtaacc actgatcagg gtcctctgcg gaagcaatta
     2521 cttgaagagg ccctggataa gaagtactcc ctacacgatt agcacaactc acttctccat
     2581 ctaaatcaat ataagcaaag ccataattag gagaaaacca ataagaaaca aaaaaacctg
     2641 attgatctgg ggcagttgta gatcctatag taacagtata gtcatcaata aattcatcta
     2701 tgcaagtcgt accaggaacg atatattttt tgagttcaag acccaagttc ttataaagta
     2761 taaagccccc tccccacaac ccatatagca aatttccatt tttatctata gaaatagatt
     2821 tcatttttga agtgatagct tctttctcaa atgtaaatgg agtaaaattt aagcgctgtt
     2881 ttggtgtcca tgtaaaccca tcgcttaatg aaatatctcc atagggggaa gcagctaaaa
     2941 cgccacctgt attcaaagtg atagtttgat gagcaggacc taaagaataa aaattccacg
     3001 catatacatc agccatttta gacccttccc tatatatcac agtagaaggg ccaataaaat
     3061 aatgaccatt ttccgttttc aaaatccatt ctaccaaact actatcattc ttaaaaagca
     3121 aagaatcatt aagctcttta tcctctaatg ttatacttgc ttgatttaag ctcgcttctg
     3181 ttttttcacc tttttcatta taataaaaag atcctctttc tgcccgtacc actaacgaat
     3241 ctttaagcca tgccatcgct cggattgtat ctggaggaag agtaattgaa tactttttcc
     3301 atgaagaagg atctgctaaa aataaatccg ctcccatatc agaccaattc atttttcttg
     3361 cataaacatt gttattgatc gctacatata gagaatcctc tttgattgtc atagcagtta
     3421 tcatctctgt atttaagcga tgagaagcaa tagatgttaa agagatcaaa gatctttcgg
     3481 aaattaaatc tataaaagcc aatttatcat taaaagctaa aaccatgaca gagtctttta
     3541 gaagaattaa ttcttttaaa acctgtgaat tacctgaaac aaaagatcta ttaataacct
     3601 taaaatttga tgttagccct gttttttttg ctattaagcc cttagcagaa accgcataaa
     3661 gtgcgttttc gttacttgct atagcataaa acatcgttgc ttccagaccg tcatcggcag
     3721 tataaacgac agattggtta tcccccaaat agaaacgaat gccaccatca gtggctaaaa
     3781 ataaatggtt ttgatagatg gttgccgaat gaatttctga gggaacagaa aaaccttcta
     3841 tcgctttaga accatacgac aaaacactcc ctaataagag aacgcataaa atctgacaat
     3901 aaatcttttt tgtttccact ggataaaatt accaaaattt aatagatgaa aacctctatt
     3961 ttaacaaaaa aggagctcta accatcatta ttaaagaaaa aattagcttt attcaatgct
     4021 ttcttaattt ttagagagac cgatgtttta gataagccta aacatttaga aatatcatct
     4081 ccattcatac ctccaataaa gtatagttct aaaatcattc gatctaaaat attaagagtc
     4141 atcaatactt gttctaactt ataaaataag gcctgttcag atttaaaatc atctccaacc
     4201 acttcataag atcccatatt ctcatttgca ataaactcgg ggcgagcttt ttgtcttctt
     4261 aaaaaatcaa gacaatgatt tctgcataca aattgaatcc atgcatcttt attgtcataa
     4321 tccttaaata catggacatg cttaaatatt tttataaaaa cttcttgttg taaatcttta
     4381 gccgtttctt gatcattaca aatctttaag caattttggt aaatcctttg ggaattttta
     4441 ttccagatcg acatcaagaa ggcttcctgc tttttcttta ctgaaaattc tatattttta
     4501 aacattatga agacttgctc ctatagctcc atcattttta ataagaatca cagcgctttt
     4561 aaatttacga aaaaaaataa aaaaaagaat agctggcaga attttgacgt ctcaagacgt
     4621 caaagcttta ttttcctagt tactttgtac ataatgtcta ttttttcctt tgctcaagat
     4681 acatcaatgg aaatagatac aagtactatg cctcctccag gaatcgaact atcttatcca
     4741 aataaatctc tcgctgtagg tgattgtgtt attgtcgaaa tcgtaattcc tgaaaaatgg
     4801 cacgtcaatg caaatgttgt tagtgatgaa tttctaaaag cgtcacaaat agaaattgca
     4861 gcaaagggca tcgttttcaa tcaacccata tggcctactt ataaaaaaaa gtataatgat
     4921 gctttagaaa tggatatttt tgtttttgat ggaaaaattc aaattaaaat tcctataaaa
     4981 gaattagaag catcttatga ttcaacaacc acaaatacaa cattccatta tcaagcatgc
     5041 agtaacttta tttgtctagc cccaaatcaa gttcatttct ctttgtcgcc ttctgcagca
     5101 acacccatcc cgttaaaaaa aaacgactct agcattttag tccttttatt ttttgcgttt
     5161 attggcggat ttattttaaa tctgatgcct tgtgttctac ctgttttatc tcttaagctt
     5221 ttcagtctca taaaacaatc aggtaaatcc agaaaacgtc tattcacact agggctttcg
     5281 atgactggcg ggatcctttt ctctttttgg attctcgcca ccattatcat ctcactaaaa
     5341 tcggcgggta gtctagtggg atggggaata caatttcaaa gcattggctt tatagccttt
     5401 atggttatct tgttaagtgc atttgcaatg aatttctttg gtttttttga gatttggtta
     5461 cctggaaaaa cactcacaaa aatggatgga ttcactaaaa aagaaggtct tgctggtgct
     5521 tttttcacgg gatcccttct tgttctttta agtacacctt gctcggctcc cttcttaggc
     5581 tcagcgatgg gatttgcctt tagtgcttct gctcctgttt tgtttttatt tttcaccgta
     5641 gcaggactag gtcttagttt accgtattta atcatcacgc tatttcctgt attactttcc
     5701 atttttccaa aacctggtcc ctggatggtt tctctacaaa aatggatggg tttattatta
     5761 cttggaaccg tgctatggct tctttgggta gcaaagcaaa cagtaggatt ggaaggattt
     5821 ttcttgctca tcattttaag tttaatgagt atgggttttt ctttcttaac aggtaaaatt
     5881 gcccctccct ataaaccttt ttatagagaa attctattac tactctcttc cgtattgatt
     5941 ctcttcttca cctggcagtt tgctgtatct cctacaatag ataaagctct aaaagagaaa
     6001 aaagcggccg ctttttctct tgaaaaagat gaatctggtt ttttccctta ttcaaaagaa
     6061 caccttacat ccgctttaga agcagggcaa cctgttttac tcgatattac tgcagattgg
     6121 tgtttaacat gtaaagcaaa tgaagctgcg gtcctctcac aagaatcttt aggatatgtt
     6181 cttgataaac ataacgttgt aaaaataaaa gctgactgga cgctacaaga caaagaaatc
     6241 agtgaattat taaaaagtct cggaagaagt ggtgttcctg tttacgccat ttatcttcct
     6301 cataatcctc aaaatcctat tttgcttcct gaaattctga caactaattc cattttagat
     6361 gttctggatt cgaatcaata aactattttt aatttatgaa agcagctctc tttactaacg
     6421 aatttccgcc tgaaatttat ggcggtgctg gaatccacat ccaatactta agcaaagagc
     6481 ttcgtgctct gtgtcagatt gaagctcgtt gttttggtcc acaaaacgac gaagaagata
     6541 atctccgagc catcgggttt tctgaaaaga taaataatgc tcctctttct gatattcgag
     6601 tcaaaaaaat cttaaaacct ttggatatta atatacagtg ggctgcttca ttaaaagata
     6661 ttgatatcat tcactgtcat acttggtata gccattttgg tggcgttctt tcaagccgac
     6721 tattacagtg tcctcttatt ttaacaactc actctttaga accccaccgc ccatggaagt
     6781 cagaagcttt aggtcaaggt ggttatgata tgagctgttg gattgaaaga actgcttatg
     6841 aagcggctga tggagtgatt gcagtaagtc aagggatgaa acgcgatgtt atggacttat
     6901 atggcgttcc tgaagatcga gtccaagtaa tttataacgg tatcgaccca gatttttata
     6961 aacccacttt tgataatgcc gttttaaaaa aatggaatat tgacccttct cgtccattcg
     7021 ttctttttgt cggtagaatc actcgacaga aaggaatttc tcaactgatt caagcgattc
     7081 ctcaaatcga tccaaatgca caaattgttc tttgtgctgg agctccagac acacaagaac
     7141 tcgctgacga atgcaagaat ctgattcttg aggttcaaaa aactagagat ggcgttattt
     7201 ggattcaaga aatgatgcct cactcagagc tacgtgtatt atatagtcac gctactgttt
     7261 ttgcaacacc ttctctatat gaaccttttg gtattattaa tctggaagcc atgagttgtg
     7321 gtactcctgt tgttggaagt gccgttggag gcattccaga aatcattgtt gatggcgaaa
     7381 caggattttt agttcctctt aaagcaaaat cgaccattaa ttttgaccct attgatccaa
     7441 aagaatttca aatgcaattt gcagataaac taaataaaat tctttcaaat cctgctcttg
     7501 ctcaaaagat gggcgtagtc agtcgtcaga gagcgattaa tgttttcagc tggaaaagta
     7561 tcgcaaagca aacctttgat ttttatagct cgtgcatcga acgctataaa aaagaaggcc
     7621 gacgcgtata gtttctaatt aatgagccgc gtgaatagtg gccataaatt cggctctcgt
     7681 tttttcatct gacttaaatc ggcctaaaag ttgtgttgtt accatcacag ctccagcttt
     7741 tttaatccct cgaatagtca tgcacgtatg agacgcctca ataacaacag cgactccttt
     7801 tggatttaat tctctttgaa gtaaagtcgc tatttgacaa gtcatccgtt cttgaacttg
     7861 tggaaatcga gcataatatt cgaccgctcg ggcgagtttt gaaagcccga caatacgatc
     7921 gtttggaata taggcaatat gtgctcgacc agtaaatgga aggaaatgat gttcacacat
     7981 actagcaaac tcaatgtctt ttagatacac catctcatca tatttttcat gaaattgtgt
     8041 ttttaatatc tcttcttctt ttaattcacc agaaagccct gtcattaatt cggcatacat
     8101 tttagcgaca cgcttaggag tatcaataag accttctcta tgaatatttt ctcccatgcc
     8161 ttcaagtatc agccgaaagc cttcttccat tttttttaaa tccattatta tttctccaac
     8221 gagtagacta aaacacgcga attacgacgc gtattatagt tcttacgttt agatggtggc
     8281 aaatcatcaa tagtaccatc ttcaaaacca aattgataga accaatctag tgcttgagta
     8341 gtcaataaaa agattttttt atagccacga atttttgcgg cagataataa atgcctaacg
     8401 acagcatctc cgacaccaga cttacgatag tttgccgcca cagcaacagc agctatttct
     8461 gcagaatcat tctcataggg atgaagagct ccacagccaa gaattgcatt atcaatcaca
     8521 tagacaacat aatcatttaa tttttctaga atcgcttctt gtgttctaga aatcaaaaat
     8581 cctttttgaa tataatcttg catgatacgt aagatatcag caatatcatc aattacagct
     8641 ggccgtattg aggaatactg actagaatag accatggtgc catcaccacg actagaaaag
     8701 acttcctgaa gaacacttcc ttgaatttca ccactaatta aatggactcg gtttgcccct
     8761 actttacatg cttgaatcgc attcagcaaa taatcctttt gtgcgaaatt taaggtttgc
     8821 ggattgagat ctaaaatttc ttttgcctga tcaatatcta aagcagaaat gactccacta
     8881 tctgtcactt ctaaatactg cgttagcgag cttgttttta gcccatccat cgaaatgcca
     8941 ttctcagttc caataaagaa caatttggca acttgaagat atttgcatag ttctgtagct
     9001 aattctgtac ttgaaatatt ataagcatgg cctagtttat tccaaccaat aggaggaatg
     9061 atcggaataa agttttcagc gagcaaccgt tcaatgatat ttctttgaat gcgttcaatt
     9121 ttccctgtcc gcatgtaatc aacaccatca atgatgccaa gactccgagc agaaacccaa
     9181 ttgccttgaa taccatcaca gccaccagct gtaagatgac tcataatccg ttgtgcaaca
     9241 cctaaagaag cttgttcaat taaaggcaaa gcttcttcac tggttaaccg aatgccatta
     9301 ttaaaattga aatctatatc ccatgctttt aattgtgcat ctatactatt acgtgttcct
     9361 ggcacaatca aaactttgat cccagcctta tgcaaaaagg atatatcttt gattaataca
     9421 gggaatagag gatggttcat caaactatct tctattttca aaacaaataa ttgtccctta
     9481 aaacgagtga tataactaaa tacctcccga ataaaactgg caacttcaat tttttgttct
     9541 ttaaaatctg gaatcactgt catgctttaa atataagaaa agcgaaagga taatctttcg
     9601 ctttttcaaa aagtaacgtt ttcaactcat tttttatctg ttttggcagc tggctttgct
     9661 tctgctttgg cagctggctt tgcttctgct ttagctgcag gttttgcctc tgttttcact
     9721 tctggctttg cttctgcttt ggctgcaggt tttgcctctg ttttcacttc tggctttgct
     9781 tctgctttgg ctgcaggttt tgcctctgtt ttcacttctg gctttgcttc tgctttagct
     9841 gcaggttgtg cttctgtttt cacttctggt ttagcaagtg aatcaactgg agctattact
     9901 tcctgaggag atggttcagc aaaaacttct aatagttcca cttcaaaaac caataacgca
     9961 tttctaggga taattggttc agcaccttct tctccataac caagagcact tggaatccaa
    10021 gccttaacct tcattcctgg tttcatcaag gttaaaagtt cctgccagcc ttcaataacg
    10081 tgagccacat caaaagttaa tggttctcca cgtttaatag agttatcaaa ctcttttccg
    10141 tctaataaag agcctatgta atgtacttta actttttcag tacgttttgg agaaagccct
    10201 tgcccatcct taatgatttt atattgaaga ccacttgcag tgacgatcac ggtgctatct
    10261 aacttgtttt tcgcaagaaa ttctgtttga cgaatctttt ccttttgagc ttcttcttta
    10321 gctgtttgat ctcgtttttc ttgaacttga agcaataggg tttccaaagc cgtttctgct
    10381 tcttcatctg tcaacaaaga agcttttcca ctcatttggt gagtcatagc taagttaaaa
    10441 gcatccatat tgacatccac tagaatactt tttagtgttt ttccaacatc catacctaaa
    10501 gcgtaactat aacgttctac ttgtgtcttt aaagaatcct taatagaggc tactgcagaa
    10561 gtatcaggtt tagaaacttg ttctgtagtt ggtttcggag cactgctaca agcagtcaaa
    10621 cttgttaaag cacaagcagt tataaataat ccaaatcgtt tcattttttc ctcttgatga
    10681 aatgcagaaa taaaagttat gtactaacat aataaaatat gattaatctg attctgaaat
    10741 aggactaatc cagctctttt tttgcagata aaaagtaaga agaagcagta atattttttt
    10801 tattaacaag caagtttttt tatctaaatg cctgattagc aagtgattag catcattttt
    10861 tacagaaaaa atgattttca acaagcaacg ctgaatttac aataatattt attacaaaaa
    10921 acttgcattt atgtaaattt tttctatcat atatagtaag atgggagctt ttttgaccaa
    10981 aaagaccatc tattttttac tgaggtagta tatgagtcaa aaatttaacc aaataataaa
    11041 tgacttagaa tcaagaattg atctatttaa tcaaaataaa ctatccttgt cattaaaact
    11101 aaatcatgcc gttcaagaag attctgaaga gctcatcact aataaaacac gctctcacaa
    11161 aacaccttct tttgaaaaaa caggatggat tccaggagcc ttatcctatc catggattat
    11221 ttcgtttcta tttattcctg aaatactaaa aaatttagtt taaatctttt ttatctttac
    11281 tccattcaaa atggagtatt tatgcctgct ttaattcttg acggaaaatc ccttgctaag
    11341 caaacagaaa atcaactgaa aatcagagta gacaatcttc gtgaatcttc aggcaaaaca
    11401 cctgttctag ccacaattct tgttggaaac gaccccgcaa gcgagactta tgttaaaatg
    11461 aagggaaatg cttgtcaaag agtaggcatg gaaagtctcc gcgttttttt acctaaaaat
    11521 attaacactg aagaacttct cgataaaatt agagaattaa acgataatcc taatgttcat
    11581 ggaattcttc ttcagcaccc tgtaccaaga catattgatg agagaaaagc tttcgaaaca
    11641 atagatttga gaaaagatgt tgatggcgtg acttgtcttg gttttggaag gatgagcatg
    11701 gggcaagaag cttatggttc tgctactcct gcagggatta tttctttatt aaaacattat
    11761 gaaattcctt tggcaggaaa acatgctgtc gttgttggca gaagtgccat tcttggaaaa
    11821 ccaatggcaa tgatgcttct tgccgaaaac tgcaccgtca ctatttgtca tagcaaaaca
    11881 aataatttac ctgatttgat taagcaagct gatattttaa taggtgctgt aggaaaacca
    11941 gaattcatta aaacgtcttg gattaaaaaa ggcgctattg tcgttgacgc aggttatcat
    12001 ccaggaggcg taggagatat cgaaaaagaa gggcttgatt caattgcctc tgcgtataca
    12061 cctgttcctg gcggtgttgg tcctatgaca attaacactc tcatccgaca gaccgtcgaa
    12121 agtggtgaga aaattattaa gcctatttaa ccgactttat ctaaacgatt ttttactttt
    12181 tcttttatat tcagtatata ctgaatcaaa attatgataa aagtcacacc agcttaacac
    12241 gacatcaaaa acaagcaagt tgttttttta ctgttttatt tctgttttta aagcatctta
    12301 ttagcaaatc ccttctaaaa cgcattgatt gagcaacaaa aaaaaattgc ttattatttt
    12361 tgaaaaaaaa atcttttttt tgagaacaaa tttagaacac aaaaaaacgc atctcctttt
    12421 tttacactac tttacatgaa tcttaattta tttttggagt ggacgtatta ggatttcgct
    12481 taatgcgatt atgaggttta tatgaaaaaa aatattttgg ctttttttgc aagcattttt
    12541 ttattgttag ttatttggaa ttgtactgaa tctccaaatg agccaaaaca aacaacagat
    12601 aatgttggag ccacctttta cccagttctc acaagtggcg gttttttaat ccttccaaac
    12661 aatttggtga ctgattttag tggtagcact gttgttggtg tttataatag cgatggcact
    12721 attacttcaa gtgaaggcat agttctagaa tcaaatgtta ataaaaatgc actatcagca
    12781 attacacccg ctctttttgt aattaatcct gatggttctg taactgatat taatggaact
    12841 cctattggag taatggatcc ttctggtaat attgttcttc caaatggagc tgtttacagt
    12901 ggttctggcg accttcttat tcctccaaca atcctttcaa gtgcaacaga acccctttct
    12961 agtgcattta ttcctctttc taacggagag gttcctcttt ctagcgcatt tatccctttt
    13021 tctagcggag aagttcctta tagtagtgcc gtcataattc cgacttcttc ttctagccag
    13081 tttgtaactc ctaattctag cagtaattgg gttcctccta catctagctg gactgctcca
    13141 agcagtagct cagttccaaa aagcagttct tctgtagcaa aaagtagcag ctcacaagga
    13201 agtcaagctc caaatattaa agtaattcaa ggtggcaaat cgggttctgg atacgccact
    13261 cgttattggg atagctgtaa gcctcactgt gcctgggaag ggaaaggtgg ccccgtagca
    13321 agaacttgtc aaagagatgg aacaactcgt gctggctctg gagaatcctc tgtgtgtgac
    13381 ggtggtaatg ctggaacttg ttttgaccaa accccgatta ttataaataa cgattacgct
    13441 tatgcttttg ctgctacccc aggtggtgga aatgactgtg gcaaatgcta catgcttaca
    13501 ttcaagggta caggagcaga tgcaacgaat actccaaccg atgacgctca cagatcaatt
    13561 aaaggcaaaa agctcatcgt gatgtcgaac aacataggtt atgacgttaa tcatggtcag
    13621 tttgacatta tgattcctgg tggtggtcct ggagcattca acggctgtca gcaaatgggt
    13681 atttcttgtg ctggcgccca atatggcgga ttcttaacaa catgtaatta caagaaggat
    13741 tgcttgatta atatgtgtaa tcaagaatat gggaacaagc caagcttaaa agagggctgt
    13801 ttattccttg ccaactggat gaatgcagct aataaccctg aagttgacta tgtagaagta
    13861 gaatgtcctt cggaacttac atctaaatac tagtatatca tttattataa aactaaaaac
    13921 ctcggacata ccgaggtttt ttttattgtg ttaactcatt attttgttat atctaaccat
    13981 ttttgatgca aaagacgatt agaaaaaacg acctgttccg catcaataaa ggctaatggg
    14041 gctccatcaa tacgagttgc ctcaccacct gcctctttaa taataagagc tattgccgct
    14101 atatcccaag gataactcat ggtcatgaca aaaagatcaa gccgccctga agccacaaaa
    14161 caaccttcta ccactgctga accgaggcat ttcactctct ggaaatgatt tgcttgaatt
    14221 tcaaaatttt gcaaattgtg tttgtttatt ttatcgatat ggcctacatt aaaatctcca
    14281 ttagagacaa tagcttgaga tgcgtttgtg acatcactta catgaatagg cttcccattt
    14341 agaaaagcgc cgcgatctaa aatagctgta aacatctcct ctaatcgagg taagtaaacc
    14401 acagacaaaa gaggcttttt attaaaatga agtgcaatgg atattcccca aaatggaata
    14461 cctcgactat aattaatggt tccatcaata ggatctatta tccagagata atttggatct
    14521 tcgccctgaa ttattccagc ttcttcagta cggatataat gagttggaaa agcgtttttt
    14581 aagccttcta cgatgatctt ttcagataag taatccgctt ttgtcaccac atctttaggc
    14641 gttttataat cgacttgccc taaacggctc tgtaaatcaa gagctgaatt tccagcagtt
    14701 ctagcaagtg tttctgcaat agataaagca tcatctaaaa attctttttg gttcataaaa
    14761 tggtcctatt tgaagaggac aaagataaca ttaaaaaacc ccagccgtta ctggggttaa
    14821 aatgcttatc taattcaatg caaataatta tggtatatca ttaggtttaa taccatattc
    14881 gcgcgcttgt gctacagaaa taattcttga ttcacccgat tcaaaattac cattagtcac
    14941 ttcctcatta tatgtttcaa tagccgagtt cactgcagtt gagaaagaaa caggcatttc
    15001 gttccaagta agcgtggttt cgagataatt atatgtattc ccttcttctt ctgttttcca
    15061 acccacaaaa gcatggccag gaatagagac taaaacaaca tctaagccta tagattcaag
    15121 aatggacgca aataacaaag ccccttctat acaatttgcc tgtttcgttc gaagtacttg
    15181 tatagggtat tttgtttttt gacccacggc acctgcttgt gtattgctga cataggttat
    15241 accctt
//
LOCUS       DAOOBB010000026        14848 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_5223_length_14848_cov_4.779490, whole genome shotgun sequence.
ACCESSION   DAOOBB010000026 DAOOBB010000000
VERSION     DAOOBB010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 14848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14848
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5223_length_14848_cov_4.779490"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PLT31_02315"
     CDS             complement(<1..336)
                     /locus_tag="PLT31_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94000.1"
                     /translation="MFTDFNKTISISIEDKYVDYGFVFGSDPKKVLFIKAGLDGSMKG
                     YQNKYLDLAYEVNQKYGITVICSSNPIVRLLLKYNPIEQGLNVLDEELGNIKEKDIYY
                     MGFSKGAYYG"
     gene            complement(441..1469)
                     /locus_tag="PLT31_02320"
     CDS             complement(441..1469)
                     /locus_tag="PLT31_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94001.1"
                     /translation="MTHLTKKVTLALLGLGLLVNTYAADGDFNLTGTIQTQGEMSFKD
                     DDKVLDKFWLRANFGGKYSSENFDAQINIRMWAPNFGNTIEEKSYDKITADLYWANYK
                     WVLGGDKLNLKLGHWKTDWSEAGNFGTYIDAALKNRGLLARDYAHDAFELGWTRGISN
                     LNVMLATTSNSFSTGYVRVEENVKFSFPLELKAAYRVNAIDAMTKPAVQTHRVAAKAV
                     YSIMKDLKVYGEIGLLKTGENSDVNAATIENDIAVAPEYAQGSTYLPFYLGVSIPTAG
                     ILDGLMLEAEYLKNRDEINKDADDFAYTVSLIKKLGKAKAQLNIYSGNELKAEEVTVA
                     LRITTTIK"
     gene            1694..2380
                     /locus_tag="PLT31_02325"
     CDS             1694..2380
                     /locus_tag="PLT31_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014544883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPW94002.1"
                     /translation="MRIIPLILALFASLFAKDLVPSKTHAVLNISYTNFQDVPQTHKK
                     LVFVGQNNPNRKITVKTDSYGEASFLIPKEDTYDIMCESLTGPFKCGESPYVSIKAST
                     GGLSVVFDDTRAELTGVTFKAGSAELVESSLYTLNEAIKGLKINPDVKIEISGHTSSE
                     GGDELNQILSEERAKSVRNYMISKGIDSKRLVAVGYGPSRPKASNDTEEGRKQNRRIE
                     LSVIEEPNSP"
     gene            2485..6345
                     /gene="purL"
                     /locus_tag="PLT31_02330"
                     /gene_synonym="purI"
     CDS             2485..6345
                     /gene="purL"
                     /locus_tag="PLT31_02330"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HPW94003.1"
                     /translation="MIILRGSAALSDFRLKKLLQDFNKSELIVKSVYAEFTHIVNLSK
                     DLSNEEEQTLNKILHYGPTREAHSPKGTLFVVSPRPGTISPWSSKATDIAHICGLTSI
                     LRIERAIAYYIEFVNTPSKEVSSNVIAKIHDRMTQKVFQAFEDLDVLFHHDSPKPLVE
                     IPILTEGREALVRADRELGLALAQDEIDYLVDNFKALKRNPSDVELMMFAQANSEHCR
                     HKVFGAEWTIDGKKKDKSLFQMIKNTYECHHTNILSAYKDNAAVMAGYEGGRFYANPK
                     TNKYDWHKEPVHILMKVETHNHPTAISPFPGAATGSGGEIRDEGATGRGSKPKAGLTG
                     FSVSNLKIPGAIQPWERDFGKPSRIASPLDIMIEGPLGGAAFNNEFGRPNILGYFRTF
                     EAEVPAEKGAEVRGYHKPIMLAGGLGNIKEEHIAKGNINPKDHLIVLGGPAMLIGLGG
                     GAASSVASGSGNEDLDFASVQRENPEMQRRCQEVIDRCWALNDENPISFIHDVGAGGL
                     SNALPELVNDGGLGGNFELRKIPNDEPGMSPLEIWSNESQERYVMAVASDRLDAFDAI
                     CKRERCPYAVVGEAVPERHLTLNDSHFNNKPIDMPLEVLLGKPPRMTRNETSQKRPLT
                     NEIFPASTTIKDVAHRVLANPSVADKTFLISIGDRTVTGMICRDQMVGPWQVPVADCA
                     VTSQTLDSYQGEAMSIGERSPVAIISPAASARIAVGEALTNLAAAYVPEMGRVNLSAN
                     WMATPNYEGDGADLYEAVQAVGMELCPDLGITIPVGKDSMSMSTVWADEKGSHRVTAP
                     ISLVISAFAPCADVRKTLTPQLIKTPSKLFLIDLGRGQNRMGASIAAQVFMKVGAKAP
                     DVDSARDLRAFFETIQKLNADGKILAYHDKSDGGLYTTAVEMAFAGHTGVSLDLKSLA
                     GNELDILFNEELGAVIQVALDQVDSVREAFASAGIGDIVFEIGSLNDSYALEIGAYKE
                     DLSDLRAIWSDTTRRIAALRDNPECAESEYLLKLEKNDPGISPKINFNIQVNADFNSR
                     PKMAILREQGVNGEIEMAAAFDKAGFESIDVHMTDVLEGRVSLADFKGLVACGGFSYG
                     DVLGAGEGWAKSILFHPKARQEFESFFHRPDTFTLGICNGCQMVSNLKDLIPGAAHWP
                     RFVQNASERFEARFSSVKIEASPSVLLKDMEGSILPIAVAHGEGRAEFSSCSSADECL
                     NTGLVSLRYVDHFDRYTERYPLNPNGSPFGITGLTTVDGRALIMMPHPERVFRTCQNS
                     WHPKQWGEDGPWMRLFRNARLFVK"
     gene            6429..7442
                     /locus_tag="PLT31_02335"
     CDS             6429..7442
                     /locus_tag="PLT31_02335"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPW94004.1"
                     /translation="MRLSSFVRHFSLFLLLSSVFVSAQLMSGKNLDVIRVEKTGISQG
                     KIDSLARMLGEQQAQGRQIPPEAMTQLRWAVIDNLVGQELLKLEIKKQNIKVSATKVD
                     SLVKFFKSQFPNEEMFQKELKKTGATAAQFREKIEQQIQSETLLEKKVPYPNEPTEKE
                     RQAYWELNKKKVPINDSISGAQIILKIKKGEKAQDKNDKKELLKGLAAQVRMGKATFA
                     NLAAQYSDDPQAKKTGGVMNRFVAKSQGSAFEKAISKLKVGDISDAFETSDAIVLFML
                     TEKNDGKYDSYKHQIDYILRVNAEQERQLAIKAYLDSLGKVYKVQYLNKDYTPPEAIG
                     ASK"
     gene            7439..8543
                     /gene="prfB"
                     /locus_tag="PLT31_02340"
     CDS             join(7439..7510,7512..8543)
                     /gene="prfB"
                     /locus_tag="PLT31_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732469.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HPW94005.1"
                     /translation="MMFQTTHQGLIDLRSRIDKLWGYLDLESKEEELAVLEKQSGDPD
                     LWNNQDKAQALMKKIGALREVLESWNTVSATCNDLAELYEMAKAENSQDLFDSIEKDF
                     SSISKKVSEMEVRKMLDGPDDACPALLSIHPGAGGTESQDWALMLYRMYVRFLERENY
                     DFRVVEVQDGDDAGLKSATIEINSEYAYGMLRSEIGVHRLVRISPFDANARRHTSFTA
                     VYLYPVHEDIEFEIDMADVRVDTYRSSGAGGQYINKTDSAVRMTHLPTGIAASCQTER
                     SQIQNRETAFRMLKTMVAEHYRQEEEAKRADRMASKKKVEWGSQIRSYVLHPYQMVKD
                     LRTGEETSDTTGVLDGKLKPFINAYLLSTSEGE"
     gene            8560..10053
                     /gene="lysS"
                     /locus_tag="PLT31_02345"
     CDS             8560..10053
                     /gene="lysS"
                     /locus_tag="PLT31_02345"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPW94006.1"
                     /translation="MLDKNDQVIARLGKLDKLREMGLEPYPHHFKRSHDSESLKANKE
                     SLLKSEEKISFAGRVVRFNRKGKMCFMHLKDRFGRLQVVCAANLLEDNYEVVKLIDLG
                     DFIGVEGTMMETQSGEYSVKAAHVTMLSKAVRPLPVPKEVIDENGNKTVFHEFKDVDT
                     RYRQRYTDMALNDDVRDVFIKRSKIMQSIREYLIEKGFIEVETPTLQPIYGGANARPF
                     TTHHNAADMTLYLRVAPELYLKRCIVGGMEKVFEFSKNFRNEGMDRTHSPEFTGLEFY
                     EAYADYNDMMVHFENIYERACIAANGTTKINYQGKEIDFKAPWPRLTMAEAIEKYSGL
                     KIASLSDDDMKNELSKRGVKLDGIWSRGRAILELFECTVEEHLIQPTIIKDMPKESTP
                     LCKTHRTETGLIEQFEPYANGWELGNAYTELNDPLRQRELLEEQVARGRGGEDETHPM
                     DENFLHAIESGLPPTGGVGFGIDRMIMLLTNQQTIRDVQLFPLMKPE"
     gene            10058..11305
                     /locus_tag="PLT31_02350"
     CDS             10058..11305
                     /locus_tag="PLT31_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPW94007.1"
                     /translation="MKKLEWLIAWRYLGAQRKSLFVSLIGIFSMLGVSIGVFALIIAL
                     SAINGFEQEVTAQMIGKDAHFEIQSYNADPMYDYDALASEIRAKEPSVIASAPYIIYK
                     VGISSKKVNDGIVIYGIDAERSKEVVDIHKHMKWGVYSVDSFPDSEGNLRPSIILGSG
                     LASRLRVSVGEKILLQTFQNPEEMVGSGGPKMLAVIVSGIFETGMYEYDGNLAYVGIP
                     ELQRLLGMGDAVTGIQFRVKDHWKAGEIVDSISKWLPYPYYGIDWKVKNITLLKWMNY
                     EKFIVAAVICLIILVAAFNIISSLIMVVIDKTREIGILRSMGLSKKSIMRVFMLMGSF
                     IGVAGTLFGGSIGLALCLVQEKYRLIKLPGDVYVLPYFPMQVQVLDIVLIFVIGIALC
                     IFATLLPAWKASRLDPVGAIRHE"
     gene            11298..11996
                     /locus_tag="PLT31_02355"
     CDS             11298..11996
                     /locus_tag="PLT31_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW94008.1"
                     /translation="MNNELLLETKALTKVYEETGEKLEILRGIDFQIYKGEYLVLTGS
                     SGSGKSTFLNLVGALDKPTSGSILFNGKDLSKMNGRERDFYHRKNVGFVFQFHHLLSE
                     FSAYENVCLPARILGSTAVECKERAEALLETVGLKDRLRHLPRELSGGERQRVAVARA
                     LMNHPDLVFADEPSGNLDEKNSEMLNELFGELNKKFNQTFLIVSHDERLAKKASRRVH
                     MHGGLITTFEKEIR"
     gene            11996..14503
                     /locus_tag="PLT31_02360"
     CDS             11996..14503
                     /locus_tag="PLT31_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HPW94009.1"
                     /translation="MNGIFSDRVKKVLQLARSAAVSIGNEHVGTEHLLLGLIREGSGI
                     AIAALKKMNVETNQMATEIEEVLRSMNGTITIGSNQMIPFTGRAKAVLQASATEAKEM
                     NERYIGTEHLLLAIIKISDSIASATLSRFDVTYDKLKDEIIAIKQDVQMSSSASVGGN
                     EANEAGVPFTRTNTRSKTPILEHFSRDLTEQARRHALDPIIGREAEIERLIQILCRRK
                     KNNPVLIGEPGVGKTAIVEGLAQKIVQKKIPELLENKRVVTLDIAAMVAGTKYRGQFE
                     ERVKGLITELQRVDNSVILFIDELHTIVGAGGSEGSLDASNIFKPALARGELQCIGAT
                     TVDEYRKYIEKDAALERRFQKIMVEPPTADESIEILSGLQKKYEAHHKVTYTDDAVRA
                     AVLLSERYMNDRFLPDKAIDVLDEAGARVRLSSMALPPDLRDKERELSEIVRKKDDAA
                     IAEDFETAATQRQLEEQYSKEIELIKNEWREKRKNECLVVSQDQIRDVISSMTGIPIT
                     RLASEETKKLLNLETEIKQRIVGQDRAVEAIAKSIRRSRAGIHDTKRPMGSFLFLGPT
                     GVGKTELAKVLSVSLFGSEDAMIRIDMSEYMEKHSVSRLVGAPPGYVGYEEGGGQLTE
                     KVRKRPYAVILLDEIEKAHPDVYNLLLQILDDGLLTDSFGRKINFKNTIIIMTSNVGA
                     REVKHSSGMGFTKGSAEDDYARMESVIREEVKRVFTPEFLNRIDDQIVFYSLNKESLI
                     SIVDILLSFLQKNLSERGILLEVSQAAKTLIVDKNYDATLGARPLRRGIQQMVEDEIA
                     EGLLMGRYKDFSTIIVDVEEGKLTFKSESLSSEVPKK"
     gene            14550..>14848
                     /locus_tag="PLT31_02365"
     CDS             14550..>14848
                     /locus_tag="PLT31_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HPW94010.1"
                     /translation="MTKIPAVLIFGPPGSGKGTVGAKLAATTSLKHLSTGDIFRGIAP
                     SSENGKLIASYSSAGKLVPDTATAQIFERFIKGLIDTNKLNPDKDMLLLDGIPRTP"
BASE COUNT         4459 a         2639 c         3180 g         4570 t
ORIGIN      
        1 accataataa gctccttttg aaaaccccat ataataaata tctttttctt ttatattccc
       61 aagctcttca tccaaaacat ttagcccttg ttctattgga ttgtacttta ataaaaggcg
      121 gacaattggg ttagaactac aaataacggt gattccgtat ttttggttca cctcataagc
      181 aaggtctaaa tatttgtttt gatacccttt catggacccg tctagacctg ctttaataaa
      241 caaaactttt ttgggatcgc ttccaaaaac aaagccataa tccacatatt tatcttcgat
      301 agatattgat atagttttat taaaatcggt aaacatgcct taagatcggg taacttattt
      361 tgacttttta taaagtaaat aaaaaacctc gagaggttta atctcgaggc gtatattcta
      421 taaaatattt ttaaaacgtt ttacttaatt gtggtcgtaa tgcgaagagc gactgtgact
      481 tcttcagcct tcaattcgtt tccagaatag atatttaatt gtgcttttgc tttaccaagt
      541 ttctttatta aagaaacagt gtaggcaaag tcatcggcat ccttattgat ttcatctctg
      601 ttctttaggt attctgcttc gagcattaac ccatcaagaa tccctgcagt aggaatactt
      661 acaccaagat aaaaaggtaa ataggtggag ccttgagcat attctggagc cactgcgata
      721 tcgttttcaa tagtggcagc gttaacatcg ctattttcgc ctgtttttaa taatccgatt
      781 tcaccatata cttttaagtc tttcatgatg gaataaactg cttttgctgc gacgcggtgt
      841 gtttgcacag caggctttgt catagcatca atggcattca cgcgataggc tgctttcaat
      901 tctaatggga agctgaattt aacgttctct tcaacacgaa cgtagccagt gctgaaggag
      961 ttgctggtgg tcgctaacat cacgtttaag ttagagattc cacgagtcca acctaattca
     1021 aatgcgtcat gagcatagtc gcgagctaag aggcctctgt tttttagagc ggcgtcaata
     1081 taggtaccaa agttacctgc ttcactccaa tccgttttcc agtggcctaa tttaagattg
     1141 agtttatcgc ctcctaaaac ccatttatag tttgcccaat aaagatcagc ggtaatcttg
     1201 tcatagcttt tttcttcaat agtattacca aaattaggag cccacatacg gatgttaatt
     1261 tgagcgtcga aattttcaga actatatttt ccaccaaagt ttgctcttag ccaaaacttg
     1321 tctaagactt tgtcatcatc cttaaaggac atttcacctt gagtttgaat ggttccagtt
     1381 aaattaaagt caccgtctgc agcataggta ttgactagaa gtcctagtcc taataacgca
     1441 agggttactt tttttgttag atgagtcatt atttatctcc tgtttgagtt tctatgacaa
     1501 aattaaagta tgcttgtaat attaatgcaa gcgaattgta aaatcgaagt caaaattaga
     1561 attaaatcca ttttttttta atggataatt ttaacttcat taaaataaga agctatttta
     1621 taatagtttt tgttgttttt ttttgtgtgt ctgttgagaa aaaacaacct taattcatga
     1681 atttggagtt tttatgcgta taattccttt aattttggct ttatttgcct ctctttttgc
     1741 gaaagatctt gttcccagta aaacacacgc tgttttaaat atttcttata ctaatttcca
     1801 agatgtgcct caaactcaca aaaaactcgt ctttgttggt caaaataatc caaatcgaaa
     1861 gatcactgtt aaaacggata gttatgggga agcttctttt ttaattccaa aagaagatac
     1921 ctacgatatt atgtgcgaaa gtctcacagg cccttttaag tgtggtgaat cgccttatgt
     1981 ttcaataaaa gcaagtacag gcggattaag cgtcgttttt gatgatactc gagccgaatt
     2041 aacaggagtt acttttaagg cgggcagtgc agaattagtc gaatcgtctt tatatacctt
     2101 aaatgaagcc atcaagggct taaaaattaa tccagatgtg aaaattgaaa ttagtggcca
     2161 cacgagttct gaagggggag atgagttgaa tcaaattctc tctgaagaac gagccaagag
     2221 tgtgcgtaac tatatgatat ccaaagggat agactcaaaa cgactcgtcg ctgtgggcta
     2281 tggcccttct agacctaaag cttctaacga tacggaagaa ggtcgtaaac aaaatcgtcg
     2341 aattgagttg tctgtcatcg aagagccaaa ttctccttaa tttcaaagat tatttaatag
     2401 atgcatttag gaataagctt tattttttaa ggcttttttc tatcttttga tcgtattttg
     2461 gcctccttta tcaaggactt tttcatgatt attctccgtg gttctgccgc cctttctgat
     2521 tttcgtctta aaaaattgct tcaggatttt aataaatctg aactaattgt taaaagtgtt
     2581 tatgctgaat tcactcatat agtgaatctt tccaaagacc tctctaatga agaagaacaa
     2641 actttaaata agattttgca ttatggacct acgcgtgaag ctcattctcc aaaaggaact
     2701 ttatttgtcg tttctcctcg tccaggtact attagcccat ggagctctaa agcaacagat
     2761 attgctcata tttgcggatt aacctctatt ttacgcatcg aacgtgccat cgcttactac
     2821 atcgaatttg tgaatactcc atctaaagaa gtttcaagta atgtgattgc taagattcat
     2881 gatcgcatga ctcaaaaagt gtttcaagct tttgaagatt tggacgtgtt attccatcat
     2941 gattcaccaa agcctctcgt agagatccct attttaacag aaggtagaga ggcgttagtt
     3001 cgcgcggatc gtgaacttgg tttagcttta gctcaagatg aaattgatta cttagtggat
     3061 aactttaagg cgctgaaacg caatccttcc gatgtggaat tgatgatgtt tgcgcaagcg
     3121 aattcagaac attgtcgtca taaggttttt ggtgcagaat ggactattga tggtaagaaa
     3181 aaagacaagt ccttgttcca aatgattaaa aatacttatg agtgtcacca cacgaatatt
     3241 ttgtctgctt ataaagataa tgctgctgtg atggctggct acgaaggcgg acgtttttat
     3301 gcgaacccta agacaaataa gtacgattgg cacaaagaac cagtgcatat tttaatgaag
     3361 gtagaaacgc ataaccaccc aacagccatt tctccattcc caggagcagc cacaggttct
     3421 ggcggtgaaa ttcgagatga aggggctact ggtagaggct ctaaaccaaa agcaggttta
     3481 actggtttta gtgtatcaaa tttaaaaatc cctggagcga ttcagccttg ggaacgtgat
     3541 tttggaaagc cttctcgaat tgcaagccct ttagatatta tgattgaagg ccctcttggt
     3601 ggtgcagcct ttaataacga atttggacgt cctaatattt taggttattt ccgtacgttt
     3661 gaagctgaag ttcctgctga aaaaggcgct gaagtgagag gctatcataa gcctattatg
     3721 cttgctggcg gccttggtaa tattaaagaa gaacatattg ccaaaggaaa tattaatcct
     3781 aaagaccact tgatcgtttt aggtgggcca gcgatgctta ttgggcttgg tggcggtgct
     3841 gcaagttctg tagcgagcgg tagtggtaat gaagatcttg attttgcgag tgttcagcgc
     3901 gaaaacccag aaatgcaacg ccgttgtcaa gaagtgattg atcgttgctg ggcattgaat
     3961 gatgaaaatc caatttcttt tattcacgat gtcggtgctg gtggattatc caacgcactt
     4021 ccagaattag tgaacgatgg cggcttaggc ggaaattttg aattgcgcaa aattccaaat
     4081 gatgaaccag gcatgagccc tcttgaaatt tggagcaacg aatctcaaga acgttatgtg
     4141 atggctgtag cctccgatcg tttagatgct tttgatgcga tttgtaagcg tgaacgctgc
     4201 ccttacgccg ttgtgggcga ggctgttcct gaacgtcact tgaccttaaa tgattctcat
     4261 tttaataaca agccaattga tatgccttta gaagtcttgc ttggaaaacc tccacgcatg
     4321 actcgtaatg agacaagcca aaaaagacct cttacaaacg aaattttccc tgcgtctacg
     4381 actattaaag acgtggctca tcgtgtttta gcaaatcctt ctgtggcaga taaaacattc
     4441 ttgatttcca ttggcgatcg taccgtgaca ggcatgattt gccgagatca aatggtgggt
     4501 ccatggcaag ttcctgtagc cgattgtgct gtgacttctc aaacacttga ttcttatcaa
     4561 ggtgaggcga tgagtattgg agaacgctct ccagtcgcca ttatttctcc agcagcaagc
     4621 gctcgtattg ctgtaggcga agcgttgact aacttagcgg ctgcttatgt tccagaaatg
     4681 gggcgcgtga atttatctgc aaactggatg gcaactccaa actatgaagg cgatggcgct
     4741 gatctttatg aagctgtaca agccgttggt atggaacttt gcccagactt agggattaca
     4801 attcctgtag gtaaagactc tatgagtatg agcactgttt gggcagatga aaaaggaagc
     4861 caccgtgtga ctgctccgat ttctttagtg atttctgctt ttgctccttg tgcagatgtg
     4921 cgtaaaactt taacacctca gttaattaaa acaccatcta aattattctt aatcgattta
     4981 ggccgcggtc aaaatcgtat gggtgcatct attgccgctc aagtctttat gaaagtgggt
     5041 gcaaaagctc cagatgtcga ttctgctcgt gatcttcgtg ctttctttga aaccatacaa
     5101 aaattaaatg ctgatggaaa aatccttgct tatcatgata aatccgatgg cggtctttat
     5161 actacggctg tagaaatggc ttttgctggc cacacgggtg tttctttaga tcttaaatca
     5221 cttgctggta acgaactcga catcttattt aacgaagagt taggtgctgt cattcaagtg
     5281 gctttagacc aagtggattc tgttcgtgag gcttttgctt ctgcaggaat cggagatatt
     5341 gtatttgaaa ttggttcttt aaatgattct tatgctcttg aaattggagc ctataaagaa
     5401 gacttgtctg atttgcgtgc catttggagc gatacaacac gtcgaattgc cgcattaagg
     5461 gataacccag aatgtgctga aagcgaatac ttattaaaac tggagaaaaa cgatccaggt
     5521 atttctccaa aaatcaattt caatattcaa gtgaatgccg atttcaatag tcgtcctaag
     5581 atggctattt tgcgtgaaca aggcgtgaat ggagaaattg aaatggccgc tgcttttgat
     5641 aaagcagggt ttgaatccat tgatgttcac atgaccgatg ttttagaagg tcgtgtttct
     5701 ttggctgatt ttaaggggct cgtggcttgc ggtggcttta gttacggtga cgttttaggc
     5761 gctggcgaag gctgggcaaa gagcatcttg ttccatccta aggcaaggca agaatttgaa
     5821 tccttcttcc atcgtccaga tacgtttacc ttaggtattt gtaatggttg ccaaatggtt
     5881 tctaatctta aagatttgat tccaggagcc gctcattggc cacgttttgt tcaaaatgct
     5941 tctgaacgtt ttgaagctcg tttttcttct gtaaaaattg aagcttctcc atcggttctt
     6001 ttgaaagaca tggaaggtag tattcttcct attgctgtgg ctcatggcga agggcgtgct
     6061 gaattcagtt cttgttcttc ggcagacgaa tgcctcaata caggtcttgt gagtttgcgt
     6121 tatgtcgatc attttgatcg atatacagaa cgttaccctc taaaccctaa cggttctcct
     6181 tttggaatta cgggccttac aacggtagat gggcgtgctt tgattatgat gcctcatcca
     6241 gaacgcgtgt tcagaacttg tcagaatagc tggcatccaa aacagtgggg cgaagatggc
     6301 ccttggatgc gtttattccg taatgctcgt ctatttgtaa aataatgcga gaatacttta
     6361 tttttacaaa gtctgagaat agatattttt ctatattcgg gctatcatct tttaaataag
     6421 ggaaacatat gcgtttatcc tcttttgttc gtcatttttc tttgttcctt ctattgtcgt
     6481 ctgttttcgt ttcggcacaa ttaatgagcg gaaaaaattt agacgtgatt cgcgttgaaa
     6541 aaacgggtat ttctcaaggt aaaattgata gtcttgcaag gatgcttggg gaacaacaag
     6601 ctcaaggccg tcagattcct ccagaagcaa tgactcaatt gcgctgggct gtgattgata
     6661 acttagttgg tcaagaacta ttgaaactcg aaattaaaaa gcaaaacatt aaggtgtctg
     6721 ctactaaagt cgatagtcta gttaaattct ttaaatcaca gttcccaaat gaagaaatgt
     6781 ttcaaaaaga gttgaaaaag actggggcaa cggctgctca gtttagagaa aaaatagaac
     6841 aacagattca aagcgaaact cttttagaga aaaaggttcc ttatccaaat gagcctactg
     6901 aaaaagaacg tcaagcttat tgggaactca ataagaagaa agtgcctatt aatgacagca
     6961 tcagtggtgc tcaaattatt ctcaaaataa aaaagggaga aaaggcacaa gacaaaaatg
     7021 ataaaaaaga attgttaaaa ggtttagctg ctcaagtacg tatgggtaaa gctacttttg
     7081 ctaatttagc cgctcaatat agcgatgatc ctcaagctaa gaaaacaggt ggcgtaatga
     7141 accgttttgt agcgaagtct caaggaagtg cttttgaaaa agccatctct aaattaaaag
     7201 tgggcgatat ttccgatgct tttgaaacaa gtgatgcaat tgtacttttc atgcttacag
     7261 aaaaaaatga tggaaagtat gatagctata aacaccaaat tgactatatc ctccgtgtga
     7321 atgcggaaca agaacgtcaa ttagccatca aggcttattt ggattcttta ggaaaagtct
     7381 ataaagttca atatctcaat aaagattaca ctcctcctga agctattgga gcaagcaaat
     7441 aatgtttcaa acaacgcatc aggggcttat agatctacgc tcccgtattg ataaattatg
     7501 ggggtatctt tgacttagag tcaaaggaag aagaactcgc tgttttagaa aagcaatcgg
     7561 gggatccaga tctttggaat aatcaagata aagctcaagc gctaatgaaa aagattggcg
     7621 ctttgcgtga agttctcgaa tcctggaata ccgtttcagc aacatgcaat gaccttgcag
     7681 aactatacga aatggcaaaa gccgagaact cacaagatct ttttgatagt atagaaaaag
     7741 acttttcttc gatatcaaaa aaagtatccg agatggaagt tcgaaaaatg ctagacggtc
     7801 cagatgatgc ttgtccagca cttctttcta ttcatccagg agctggcggc acagagtctc
     7861 aagactgggc tctcatgctc tatcgcatgt atgttcgctt tttagaacgc gaaaattatg
     7921 acttccgcgt agtggaagtt caagatggag atgacgcagg gcttaaaagc gctacgattg
     7981 aaattaattc tgaatatgct tatggtatgc ttcgctctga aataggcgtg catcgtttag
     8041 tgcgtattag tccttttgat gcaaatgctc gccgtcatac gagttttact gctgtatatc
     8101 tttatcccgt tcatgaagat attgaatttg aaatagacat ggctgatgtt cgggtggata
     8161 cctatcgctc ttctggagcg ggtggccagt atatcaataa aacagattct gctgtgcgaa
     8221 tgactcatct tcctacaggc attgctgcga gctgccaaac agagcgttct caaatacaaa
     8281 accgtgaaac ggcatttaga atgcttaaaa ccatggtggc agaacattac cgtcaagaag
     8341 aagaagcaaa acgtgcagat cgaatggctt ctaagaaaaa agtagaatgg ggctctcaaa
     8401 tcagaagtta tgttttgcac ccatatcaga tggtcaagga tcttcgcaca ggagaagaaa
     8461 cttctgatac gactggagtt cttgacggaa agttaaaacc ctttattaat gcatatctcc
     8521 tttcgacaag cgaaggggaa taaccacaga agaagtatta tgttagataa aaacgatcaa
     8581 gtcattgcac gactaggaaa gctcgataaa ctccgcgaaa tgggtttaga accttatcca
     8641 catcatttta agcgttcaca tgattctgaa tcattaaaag caaataaaga atctttattg
     8701 aaatcagaag aaaaaatttc ttttgcaggt cgtgtggttc gttttaatcg caaaggcaag
     8761 atgtgcttta tgcatcttaa agaccgtttc gggcgtttac aagtcgtttg tgcggcgaat
     8821 cttttagaag ataattatga agtcgtgaaa ttaatcgacc tcggcgattt tattggcgtc
     8881 gaaggcacca tgatggaaac tcaaagtggt gagtattctg tgaaggccgc tcatgtcacc
     8941 atgctttcta aagcagtacg tcctttacct gtgccaaaag aagtcattga tgaaaatgga
     9001 aataaaaccg ttttccatga atttaaagat gtcgacactc gttatcgtca gcgctataca
     9061 gatatggcgt taaatgatga tgtacgtgat gtatttatta aaagatcaaa aatcatgcag
     9121 tcgattcgtg agtatttgat tgaaaaaggt tttattgaag tcgaaacgcc gactctacaa
     9181 ccgatttatg gtggagccaa tgctcgtcca tttaccactc atcataatgc tgctgatatg
     9241 actttgtatt tacgcgtggc tccagagctt tatcttaagc gctgtattgt tggcggtatg
     9301 gaaaaggttt ttgagttcag taaaaatttc agaaatgaag ggatggaccg tacacatagt
     9361 cctgaattta caggtcttga attttacgaa gcctatgctg attacaatga tatgatggtt
     9421 cattttgaaa atatttatga acgcgcttgt attgcagcaa atggaacaac aaaaatcaat
     9481 taccaaggga aagaaattga ttttaaagca ccttggcctc gtctcactat ggctgaagct
     9541 attgaaaaat atagcggatt aaaaattgct tcgttaagtg atgatgatat gaaaaacgag
     9601 ttatctaaac gaggtgtaaa actcgatggg atatggagcc gtgggcgagc gattcttgaa
     9661 ctatttgaat gtactgtaga agaacatttg atccagccta caattatcaa agacatgcca
     9721 aaagaaagta cgcctctttg taaaacgcat cgcacagaaa ctggtcttat tgagcagttt
     9781 gagccttatg ccaatggctg ggaattagga aacgcttata ccgaattaaa cgatccttta
     9841 cgtcaacgtg aattgcttga agaacaagtc gctcgtggcc gtggtggtga agatgaaacg
     9901 cacccgatgg atgaaaattt tcttcatgcg attgagtcag gccttcctcc tacaggcggt
     9961 gttggctttg gtatcgatcg aatgataatg cttctaacaa atcaacaaac aattcgtgat
    10021 gtacaattgt tcccgctgat gaaaccggaa taaaaaaatg aaaaaattag aatggctcat
    10081 tgcatggcgt tacttaggag ctcaaagaaa aagtcttttt gtttctttga ttggcatctt
    10141 tagtatgctt ggagtgagca ttggtgtttt tgctttaatc attgctcttt cagcgattaa
    10201 cggctttgaa caagaagtca ccgcccagat gattggtaaa gacgctcatt ttgaaatcca
    10261 gagctacaac gctgatccga tgtatgatta cgacgccttg gcttcagaaa ttcgggctaa
    10321 ggaaccttct gtgattgctt ctgctccata catcatttat aaggtaggca tcagctcaaa
    10381 aaaagtcaat gatggtatcg tgatctatgg aattgatgct gagcgttcta aagaagtagt
    10441 ggatattcat aaacacatga aatggggcgt ttattctgta gatagttttc ctgattcgga
    10501 ggggaatttg cgcccttcca ttattttagg aagtggtctt gcaagtcgct taagagtttc
    10561 tgtcggagaa aaaattttac tacagacttt tcaaaatcca gaagaaatgg tggggtctgg
    10621 tggacccaaa atgttagccg tgattgtctc tggtattttt gaaacaggaa tgtatgaata
    10681 tgacggtaac ttagcctatg tagggattcc tgaactacag agattgttag ggatgggaga
    10741 tgcagtcact ggaattcaat ttcgcgtgaa agaccattgg aaagctggag aaatcgtgga
    10801 ttctataagt aaatggcttc cttaccctta ttatggaatc gactggaaag tcaaaaatat
    10861 cacccttctt aaatggatga attacgaaaa gtttattgtg gctgctgtca tttgtttaat
    10921 tattctcgtg gctgccttta atattattag ttctttgatc atggtcgtga tcgataagac
    10981 tcgtgaaatt ggtatcttaa gaagcatggg actcagtaaa aagagtatta tgcgtgtgtt
    11041 tatgttgatg gggtctttca ttggcgtcgc tggcacttta tttggtggaa gtattgggct
    11101 tgcgctttgt cttgtccaag aaaagtatcg cttgatcaaa ttacctggtg atgtatatgt
    11161 gttaccttat tttcctatgc aagtgcaggt tttagatatc gttttgattt ttgttatagg
    11221 aatagctctt tgtatttttg ccacattatt accagcttgg aaagcaagcc gtttagatcc
    11281 agtaggagcc attcgtcatg aataatgaat tgttattaga aacaaaggct cttacaaaag
    11341 tttatgaaga aactggggaa aaactcgaga ttcttagagg gattgatttc caaatatata
    11401 aaggcgaata tcttgtttta acaggttctt ctggatctgg aaaatcaacg tttcttaatc
    11461 tagtaggcgc tttagataaa ccaacatcgg gctcgatttt atttaatggc aaagacttaa
    11521 gtaaaatgaa tggaagagaa cgtgattttt atcatagaaa aaatgttgga tttgtttttc
    11581 aatttcatca cttgctttct gaattttcag cgtatgaaaa cgtgtgccta cctgcacgta
    11641 ttttaggctc tactgcagtg gaatgtaaag agcgtgctga agcgttgctt gaaacggttg
    11701 gcttaaaaga tcgtctacgt catttaccgc gagaattatc tggtggagaa cggcagcgtg
    11761 tggctgtagc aagagctctc atgaatcatc ccgatcttgt ttttgcagac gaaccgagcg
    11821 gtaatttaga tgaaaaaaat tcagagatgc ttaatgaatt atttggcgaa ttaaataaaa
    11881 aatttaatca aacattttta attgtctctc atgatgaacg tttagcgaaa aaggcttctc
    11941 gtcgtgtgca tatgcatggg ggattaataa cgacctttga aaaggagatt cgttaatgaa
    12001 cggcatattt tcagatcgtg taaaaaaagt tcttcaactc gctcgttcag ctgcggtgag
    12061 catcggaaat gagcatgtag gaacagagca tttgctcttg ggcttgattc gtgaaggctc
    12121 tggtattgct attgcagcct taaaaaagat gaatgtagaa acgaatcaaa tggcgacaga
    12181 aatagaagaa gtcttacgtt ctatgaatgg tacgattacg attggaagca atcaaatgat
    12241 tccttttact ggtcgtgcta aagcggtatt acaggcttct gcaacagaag caaaagagat
    12301 gaatgaaaga tatattggaa cagaacatct cttgttagcc attataaaga ttagtgattc
    12361 tattgcttct gctactttat ctcgtttcga tgtgacttat gataaattaa aagatgaaat
    12421 tattgcgatc aaacaagatg tgcaaatgtc atcttctgct tctgtcggtg gaaacgaagc
    12481 taacgaagct ggagttcctt ttactcgtac taatactcgt tctaaaacgc caatactaga
    12541 acattttagt cgtgatttaa cagaacaagc gcgtcgccat gctctagatc caatcattgg
    12601 tcgagaggca gaaattgaac gcttgattca aattttatgt cgccgtaaaa agaataatcc
    12661 agtcttgatt ggagaacctg gtgttggtaa aacggctatt gtagaagggc ttgctcagaa
    12721 aatagttcag aaaaaaatac cagaactttt agaaaataaa cgcgtagtaa ctttagatat
    12781 tgctgcgatg gtcgctggta caaaataccg tggtcagttt gaagagcgcg tgaaaggatt
    12841 aatcactgaa ttacaaagag tagataattc ggtcattttg ttcattgatg agttacatac
    12901 aattgttggg gctggcggtt ctgagggtag tttagatgca tcgaatattt ttaagcctgc
    12961 tttagcacgt ggagaactcc aatgtattgg tgcaacaacg gtagatgaat atcgtaagta
    13021 catcgaaaaa gatgctgctt tagaaagacg attccaaaaa atcatggtag agcctcctac
    13081 tgctgatgaa tcgatagaaa ttttgagcgg tctacagaaa aagtatgagg cccatcataa
    13141 ggtcacttat acagatgatg ctgtgagagc ggctgtactc ctttctgaac gctatatgaa
    13201 tgaccgtttc ttaccagata aagcaattga tgttttagat gaagctggcg ctcgcgttcg
    13261 tctttctagt atggcattgc ctcctgattt aagagacaaa gaacgtgaac tttcagaaat
    13321 cgttcgtaaa aaagatgatg cagctattgc tgaggatttt gaaacggctg cgacacagcg
    13381 tcaattagaa gaacagtaca gcaaagaaat tgaattaatc aaaaatgaat ggcgcgaaaa
    13441 gcgtaaaaac gaatgtttgg tcgtctctca agatcaaatt cgtgatgtca ttagcagtat
    13501 gacaggcatt ccaattacta gactcgctag cgaagaaaca aaaaaacttt tgaatctaga
    13561 aactgaaatt aaacaacgca tagttggcca agatagggcc gtagaagcga ttgcaaaatc
    13621 cattcgtcgt tcaagagcag gtattcatga taccaaacgc ccgatgggta gtttcttatt
    13681 cctaggccca actggtgttg gtaaaacgga attagccaag gtgttaagtg tttccctttt
    13741 tggctcagaa gatgccatga ttcgcattga catgagtgaa tatatggaaa agcacagcgt
    13801 ctctcgttta gtaggtgcgc ctccaggata cgtcggttat gaggaaggtg gtggacagct
    13861 cacagaaaaa gtgcgtaagc gtccttatgc cgttatcttg ctagatgaaa ttgaaaaggc
    13921 tcatcctgat gtctataatt tgctattaca aattttagat gatggccttt tgaccgatag
    13981 ctttggccgt aaaattaact ttaagaatac aatcattatc atgacttcga atgtgggtgc
    14041 tcgtgaagta aaacacagct cgggaatggg ctttactaaa ggttctgctg aagatgatta
    14101 tgctcgcatg gaatctgtaa ttcgagaaga agtcaagcgg gtctttaccc cagaattttt
    14161 gaatcgtatt gatgatcaaa ttgtattcta tagcttaaat aaagaaagtc ttatttcgat
    14221 tgtagatatt ttactttcat tcttgcaaaa gaatctttct gaacgtggaa ttcttttaga
    14281 agtctcccaa gctgcaaaaa ccttgattgt ggataaaaat tatgatgcca cattaggtgc
    14341 tcgtccgctt cgtcgaggaa ttcagcagat ggttgaagat gaaattgccg aaggccttct
    14401 catgggtcgc tacaaagatt tttcaacgat tattgttgac gtagaagagg gtaaattgac
    14461 tttcaaaagt gaaagtttat catcggaagt cccaaaaaaa taattatttt tgcgacgcta
    14521 cattttcatt ttaactaatc gaggatttta tgacaaagat tcctgcagtt cttatttttg
    14581 gccctccagg ttctggtaaa ggaaccgttg gtgcaaagct tgctgctacc acttctctta
    14641 agcacctttc tacaggtgat atctttcgcg gcattgcgcc ttcaagtgaa aatggaaaat
    14701 taattgcatc ttattctagc gctggaaagc ttgttccaga tactgctacc gctcagattt
    14761 ttgaacgctt catcaaaggt ttaattgaca ctaataaatt aaatccagat aaagatatgc
    14821 ttttactcga tggtattcct agaacacc
//
LOCUS       DAOOBB010000027        14713 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_5283_length_14713_cov_4.469368, whole genome shotgun sequence.
ACCESSION   DAOOBB010000027 DAOOBB010000000
VERSION     DAOOBB010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 14713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14713
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5283_length_14713_cov_4.469368"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..1478)
                     /gene="asnS"
                     /locus_tag="PLT31_02370"
     CDS             complement(180..1478)
                     /gene="asnS"
                     /locus_tag="PLT31_02370"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF003483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HPW94011.1"
                     /translation="MIQKTYISHLTSKKNETVELAGWVVKVRDSGKICFMQFRDGTGF
                     IQVVATPNDMSAEEFEIMRHLSIESAVKIKGIVAENVKSPSGIEIIAKELTLVSKTEN
                     YPITPKEHGTDFLMDIRHLWIRSPRQHAILRIRHQIITAIRHYFDDLDFTLTDAPIFT
                     ANECEGSSTLFTTEYFGENAYLSQSGQLYMEAACQAVGRAYCFGPTFRAEKSNTRRHL
                     TEFWMVEPEVAYMDLEGCMELAEGLIQAIVKRVLEHCPQELKILERNLEPLESVIAGP
                     FPRISYDEAVEIIKKSGTHFEYGDDFGGADETIISNAFNRPVIVHSYPTAIKPFYMKK
                     DPKNPSVVRNMDVLAPEGYGEVIGGGQREEKYETLLESILASGLNPETYSWYLDVRRF
                     GAVPHAGFGLGVERCVAWITGISHVRETGLFPRMPQRLRP"
     gene            1542..2288
                     /locus_tag="PLT31_02375"
     CDS             1542..2288
                     /locus_tag="PLT31_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94012.1"
                     /translation="MFSTLKKQILLFMGLLILSSPIAAMEDIPLPKQSAFTGKGLAIG
                     IGVGVFNPSGDDCNCIGMWQAHVDYFYLPYLSGGADIRFYGGDVDRHSMIIYQRYQLH
                     LRYHKKIMENLAFYGAPLIGFETTDLHEIKKNITTGEPIDTELEEDVCTESFALNGFS
                     MGFDLGVGLKVNKDFGLVTGALYEFNFSDFHYMALTAGVTFNIRNHWKRLEEGMLSSW
                     LSLEIQWQRYLGTGDGLWSETILLGLFFGV"
     gene            2346..2783
                     /gene="rpsF"
                     /locus_tag="PLT31_02380"
     CDS             2346..2783
                     /gene="rpsF"
                     /locus_tag="PLT31_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPW94013.1"
                     /translation="MSVLTEPSQNHGDFGLTTKGKINMRQYETMVIIDAMISDEAIDA
                     EVQAMASIISAGGEILRRDDWGKRKMAYSINKKTHGYYVIFYYKAEVSVVAALEAAFK
                     HNDNLLRWMTLVDYPMTEIIYDKNLVQTTEDITPIDVDDVEVD"
     gene            2783..3034
                     /gene="rpsR"
                     /locus_tag="PLT31_02385"
     CDS             2783..3034
                     /gene="rpsR"
                     /locus_tag="PLT31_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPW94014.1"
                     /translation="MSFEDKKQGARVRRKKTCWFTENNVQFIDYKDEKILRRFVSERG
                     KIIPRRISGTSAKYQRMLNQAVKRARQMAILPFVADSIR"
     gene            3044..3490
                     /gene="rplI"
                     /locus_tag="PLT31_02390"
     CDS             3044..3490
                     /gene="rplI"
                     /locus_tag="PLT31_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HPW94015.1"
                     /translation="MEVILKANVAKLGKMLDIVKVKDGYARNFLFPKKLAMRATKAAK
                     LEIQRNREAMEALFRKELAAAGDLAAKLSEVTVNLTRRVVEDERLYGSVSAADIAEAI
                     TLQGIKINRNQVILPEPIKQLGVFSVAINVFGDVEATVKVWVVKEN"
     gene            complement(3559..3903)
                     /locus_tag="PLT31_02395"
     CDS             complement(3559..3903)
                     /locus_tag="PLT31_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94016.1"
                     /translation="MFTEEELDDTGIEITDVSATGVSGATCEELDDTGFSEIEELDKT
                     GTSAEEEEISFIEEELDDKGLSEIEELDKTGTSSEEEDEGLTSASNSAKRSLTWAKEE
                     VEKIIMSRKNNL"
     gene            3902..4864
                     /locus_tag="PLT31_02400"
     CDS             3902..4864
                     /locus_tag="PLT31_02400"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HPW94017.1"
                     /translation="MVSSSSIQVVAPIASSSSMSRRDLLGPVKVSKIRGINEMKGSYK
                     SPRKALFMSLVLPGAGQIYVGTSTFNKVRGGVYLALEAALWSGWYYYSVHKYNNQVSR
                     YKDYANTHFSVGAYEQEMHNIYLLQLEDASEEANFRIRYLNSRESYCKSIYGTATTNN
                     CYSSENLFEKDSRHLDLFANVSLGESKKGTSFYDDNQFYQLIANPIFVLGWDDVTDRA
                     SYQQLGLSDSQSETVPLGTSSNLSTYQDMRDKANDYASMQAWFFGGLILNHVISAFDA
                     ALSANANNKLLYEEKLSWLDQMHLGSSVSFFDGFMASVQAYWNF"
     gene            4864..5586
                     /locus_tag="PLT31_02405"
     CDS             4864..5586
                     /locus_tag="PLT31_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94018.1"
                     /translation="MKKKIFCFVLLCSVSLWAQTVISIDESISRNQNKSIALSLGASL
                     LLPGTGEYYLGEKQLLRPFIWTDAALWITTIGSYFIGEKYISSAHNYAVTHAGVSSTK
                     KSVDFLNTVADYRSRGGVFGQNSNPDMNEDYNQAMIRSGKSVDEEYPNTMEYQWDWGS
                     SDYPENTEHMDIYKNMLRNYRVSRIVFQVSVGALILNRVVSFLDVMRIYRATSSKKMT
                     RRWDIAPQFYPDGSGAVFSYQF"
     gene            complement(5591..7228)
                     /locus_tag="PLT31_02410"
     CDS             complement(5591..7228)
                     /locus_tag="PLT31_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94019.1"
                     /translation="MRFLFFVLFLLFSWSFGLRPTCGTEKFIKHQKQIKTGLLSRSIA
                     QSALTSCPEESYYDKIDTILTKHFVIYYTLEGPHATTESFLDSLTFYLEKAWNLHTTK
                     LGMKPPKAMSRSYHYKQENPLGLYPVEVIDIDLLRDTDNLMGGSCHGCFGLTYPGDPE
                     NPEATILMIDNDFKYTPEYNQQEAWITDSKGKECSYPIATKELYNNNQTSYSYFDYPE
                     KAIQVTAFHELYHAAQIRYMSFIDYPSFWFEASATGVEEIGAKEVNDYWGYLYDIFDY
                     PGTALSALNQDYSAVVFYLYLYENNGPLFDASIWEAYSKNPEKPFYEILSKKWVAQGI
                     EPDSAFHDFAKQLFFTNTRSKYKPTDSLYYEDMPHWPEMTIRPYNEKNQNLSSLAFNY
                     LSIKSSMPDISYFKGQASLFFWNSDDSEVSIKSLLNADDIDLWRSTISESDSAILVLS
                     RFSDDSPTIVPLVAKPLRAYPTPWQGTTELCFSPLPNTRSFIEIRNRIGNLVIRIPTN
                     STTHCLKPDYIKSIMSPGLYHFRVGSHGKTTPLLLIY"
     gene            complement(7230..9038)
                     /locus_tag="PLT31_02415"
     CDS             complement(7230..9038)
                     /locus_tag="PLT31_02415"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HPW94020.1"
                     /translation="MSYKSLEDALIDLKKAGMLLTISEEVDPYLEMASIARQAYKRKA
                     PAILFEKVKGSPFRAACNIFGTLERAEFLFRKEWKTLETAISAKANPLSVFQAPQNLP
                     LLPFAAIQAKPQRSFQVPVLKEECSLSDLPQLHSWKEDGGAFITLPQVCSLDPEESSL
                     LKANLGMYRIQISGNDYIANQECGLHYQINRDIARHHQKAIILKKPLKVSIFIGGPPA
                     HTLAAVMPMPENLSELIMAGLLNGRAFRYSMHEGWVISADADFCILGEIDILQKPEGP
                     FGDHLGYYSAKHNFPFLKVSKVFHKKNAIFPFTVVGRPPQEDTVFGHLIHKITAPMVP
                     ASIPGLMEMHAVDAAGVHPLLLAIGSERYVPYRPSEPMELLKTSNALLGFNQAALAKY
                     LLIAACEDDPSLTTKNIPAFFSHVLSRLDFSRDLHFQTSTTIDTLDYSGTSLNHGSKL
                     VLAAAGKPKRELGNAQGEVFQTFPKVEGFSNLKYVMPGVLALEAKSRASMEALARALE
                     SWEHKELYPWISIVNDASFTSESIENWLWITFTRSDPAQDIYGVRHHIHYKHFACEAP
                     ILIDARIKAHHQRPLETNFEVEDRAEQKLLKALKRA"
     gene            complement(9078..9566)
                     /locus_tag="PLT31_02420"
     CDS             complement(9078..9566)
                     /locus_tag="PLT31_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94021.1"
                     /translation="MVTFSQTAWFETSKEELINTLQIAPFHLKMESTPIVHYHMPEGT
                     ATGATVHYHLVAGFNRISWTGIISSSQNNQITTRLGKGPFRGFNAKHSILNDGHLSVC
                     YEELSFQGEEDSFRTEIEKARILYGLEERKKTLKMLMAYESKKKTKSFESLDCFGSST
                     AG"
     gene            complement(9804..10472)
                     /locus_tag="PLT31_02425"
     CDS             complement(9804..10472)
                     /locus_tag="PLT31_02425"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPW94022.1"
                     /translation="MPPRGVIFDLYGTLFIYKNMGKAFFSWHESLALGAKELGFSTSA
                     EQMAKECETFFSFPVTRLFNITPYEERLLFLFKKIGANPSLDWICDFSNRSMDSWQKE
                     MVLHPEAIPFLDELKRANVKTAILSNFDHYPHVYRFLKQTKLSDLMDAIVISSEVGLK
                     KPDAEIFEYTLQKLNLSASSTLMVGDDYKKDIEGAKKFGLMTHYHQNGASFAPIRSLF
                     NKNF"
     gene            complement(10473..10775)
                     /locus_tag="PLT31_02430"
     CDS             complement(10473..10775)
                     /locus_tag="PLT31_02430"
                     /inference="COORDINATES: protein motif:HMM:NF019491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HPW94023.1"
                     /translation="MIKHIVLWKMKDNAEGASAIENATKIVDMLHDLKGKIPSLISIE
                     AGIDFNRSGAAWDLALYSSFQNTEDLNNYQIHPEHQKVATFIRAVTEDRCVVDFEI"
     gene            complement(10772..11953)
                     /locus_tag="PLT31_02435"
     CDS             complement(10772..11953)
                     /locus_tag="PLT31_02435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPW94024.1"
                     /translation="MTIIDFNNFWSPSGGGVRRYHLEKMKYYTSESKNLLVFVMPDSK
                     TYTEEVSSSLIVEHVKAFKMPLYNGYRFIWKKKQIAPYLKKYNPDVIEVGSPYILPKA
                     VSKQALQICPQAKLLGFWHADFPITYVQRAFTRFFGKKIGAFARKLAFLYAHYQFKNY
                     HGIQVSCKEIIERLESEQFKNLFWIPLGCNIELFSPNKRDEALVKELKQGDEKRSIIF
                     FPHRFSKEKGLDLFLKAYSILAEKMNPVPAVVFAGTGPLQLLIDVALKKYKHLHYVGF
                     LSSEEEMARYYASANIGVALSAWETFGLSILESMACGNALIGASAGAAREHVSSSGAG
                     LILESYKADALAKCLLELCEQDLTQKQKAARLYAEKFSWDNCFSNQLKIYSTIISSRK
                     S"
     gene            complement(11956..13830)
                     /locus_tag="PLT31_02440"
     CDS             complement(11956..13830)
                     /locus_tag="PLT31_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HPW94025.1"
                     /translation="MDKDRTGLQSIKTHPRHLGIPLSRWGRNLISCCPFHAPEETSLF
                     FYDALGYWRYRCLQCGSEGDLVEFIMRSRFNGLDDQAARIEALEFFGAQEQADPDKSE
                     YEHPWVKEIGGEKSKVLEQFVSYCHWAACKSQSSAEFLAKRGWNIGQAQLYGIGYYSG
                     DPEPFISYSMLAGVERHQINFYLDNLEVYREPRITIPARNSKGIIHSVYGRSMEENHP
                     HPYISYASGPADIPFNIQADNERPVIVEGFFDALTADLAGIAGVVSTMYQELTQSHLY
                     KLKACGAESVTVILRRDANRRQQEYQIQKYLKLAENLDLKFKSIILPKDETIDQIVRK
                     NGADQLLELITHTEEDTIHTHRRSVMLQDIKENFDNAMACPPTTSVGYSLSNFPKLDA
                     TIDGIQSGCFFISSQPFGLKSLLLTSLALDLVESNPKIKVIYIGLETPRRQLFDRMIA
                     MMTGESILTVKKQNANDETNKKILEATKEMISYVRQNRLEIWEDEPGFDNVEILRILK
                     EELKEHQDLAVIIDGIDHLKITDRNDISDINERRSTVMLDLYKALDIPIFLGGELLCT
                     ESGLVGPRPYLRDSDAIFWLESKGGNLFLSVDSKRLGKNHLYQGNIIMDSISNRMNEE
                     "
     gene            13965..14531
                     /gene="ruvC"
                     /locus_tag="PLT31_02445"
     CDS             13965..14531
                     /gene="ruvC"
                     /locus_tag="PLT31_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPW94026.1"
                     /translation="MVILGIDPGSHTTGYAFLETAPQWIRVLEYGCIRASSTKSPEMR
                     LLDIITDLEALINQYKPTALSMEGIFFAKNIKSALLLGHVRGAILAICLKRGMTYHEY
                     SPKSVKLAVTGSGSSSKERVCQMVQVHLQLKEIKGPLDASDALAIAWTHAHPVTTVSK
                     PACLVKKKRKNQAQEWRSLLKKCGGLSE"
     gene            14524..>14713
                     /locus_tag="PLT31_02450"
     CDS             14524..>14713
                     /locus_tag="PLT31_02450"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HPW94027.1"
                     /translation="MNEVVSKLAPFGLDEEGLVSLYPEKLLLRKEAFNALEQLRQLAR
                     LFGFDIRVESAYRSFDRQI"
BASE COUNT         4432 a         2607 c         2934 g         4740 t
ORIGIN      
        1 ttaccgtcaa caatggttat ttagatttaa tcgtgaaaaa atcattaccg tcaaccatag
       61 tattattgag ttttaatgaa aataccattt caatcaataa cagtagtttt gagacataat
      121 gaaaaaccat tcacgtaaac aaagtgacat tgaaacgaaa tagaaaaaaa caacaaaacc
      181 tatgggcgaa gcctttgtgg catccttggg aataagccag tttctcttac atgagaaatc
      241 cctgtaatcc aagccacaca acgttcaacg cctaaaccaa aacctgcgtg aggaacagct
      301 ccaaagcgac gtacatctaa ataccaagaa tacgtttcag gatttaaacc agacgctaga
      361 atcgattcaa gaagggtttc atatttctct tctcgttgcc cgcctccaat gacttcaccg
      421 tatccttcgg gagcaagaac atccatgttt cgaacgacgg aagggttttt cggatctttt
      481 ttcatgtaga aaggcttaat ggccgttggg tagctatgaa cgatgactgg gcgattaaaa
      541 gcgttactga taatggtttc atcggcgcct ccaaaatcat ctccgtattc aaaatgagtt
      601 cctgatttct tgataatttc aacggcttca tcatagctta ttctcggaaa aggccctgca
      661 atcacggatt caagaggttc taggtttcgt tcaagaattt ttagctcttg tgggcaatgt
      721 tctaaaacgc gtttgacaat tgcttgaatt aaaccttcgg caagttccat acaaccttct
      781 aggtccatat aagcaacttc tggttctacc atccaaaatt ctgtgagatg tcttcttgtg
      841 tttgactttt cggctctaaa cgttggacca aagcagtagg ctcgacccac ggcttggcat
      901 gcggcttcca tatataattg accgctttga gaaagataag cgttttctcc aaagtattct
      961 gtggtaaata aagtgctgga accttcgcat tcgttcgctg taaagatagg ggcatctgta
     1021 agagtaaaat ctagatcatc aaaataatgg cgaatggctg taataatctg gtgacggatt
     1081 cgaagaattg catgttgcct agggcttcta atccagaggt ggcggatatc cattaaaaaa
     1141 tcggtaccat gttctttggg ggtaataggg tagttttctg ttttagaaac aagtgttagt
     1201 tctttcgcga taatctcaat gccagaaggg gattttacat tttctgcgac aatccctttg
     1261 atttttaccg ccgattcaat ggataaatgt ctcatgattt caaattcttc agcactcata
     1321 tcgtttggtg tcgcgactac ttgaataaag ccagtaccat cacgaaattg cataaaacaa
     1381 attttaccag agtctcgtac ttttacaacc cagccagcaa gttcaactgt ttcgttcttt
     1441 ttagaagtca aatggcttat ataagttttt tgaatcatat ctaacctcaa cattttgcta
     1501 aaagatagtt ttatcttaaa tgatttctta gatttgtaat tatgttttcc actctaaaaa
     1561 aacaaattct tctttttatg ggtctcctta ttttaagtag tcctatagca gcgatggaag
     1621 atattcctct tccaaagcaa tctgctttta cgggtaaggg tttggctatt ggtattggag
     1681 ttggcgtatt taatccgagt ggggacgatt gtaactgtat tggaatgtgg caagctcatg
     1741 tggattactt ttatttgcca tacttaagtg gtggtgcaga tattcgtttt tatgggggag
     1801 atgttgatcg tcatagcatg attatatatc aacgatacca gttgcatctt cgttatcata
     1861 aaaaaattat ggagaattta gctttttatg gagccccttt aattggtttt gaaacaacgg
     1921 atctccacga gattaaaaaa aatattacca ctggagaacc tatagatact gaattagagg
     1981 aagatgtttg taccgaatct tttgcgctaa atggattttc aatgggcttt gatttaggtg
     2041 ttggtcttaa agtcaataaa gattttggat tagttactgg agccctttat gagtttaatt
     2101 tctctgattt ccattatatg gcactcacgg cgggtgttac ttttaacatc aggaaccatt
     2161 ggaagaggtt agaagaaggc atgctttcca gttggctctc tctagaaatc caatggcaac
     2221 gttatttagg aactggagat ggtttgtggt cagaaaccat tttgttaggc ctgttttttg
     2281 gggtctaatt cgccttataa accttatttt tattgacaat acttgaattt tgtcctaaat
     2341 ttggcgtgtc cgtattaacg gaaccctctc aaaatcatgg tgattttggg ctaacgacca
     2401 aagggaaaat aaacatgaga caatacgaaa cgatggttat catcgatgct atgatctctg
     2461 acgaagcaat tgatgctgaa gttcaggcca tggctagtat tatttcggcg ggtggagaaa
     2521 tccttcgtcg ggatgattgg ggtaagcgca aaatggctta ttctattaat aaaaagacac
     2581 acggctacta tgtaattttc tattacaagg ctgaagtttc tgtcgttgct gctcttgaag
     2641 ccgctttcaa acataatgat aatcttcttc gttggatgac tctcgttgat tatccaatga
     2701 cagaaatcat ttatgacaag aatttagtac agactacaga agacatcact ccaattgatg
     2761 tcgatgacgt ggaggtagac taatgtcttt cgaagataag aaacaaggtg ctcgcgttcg
     2821 tcgcaagaaa acttgctggt ttacagaaaa taacgttcag ttcatcgact ataaagatga
     2881 aaagatttta cgtcgcttcg tttcagaacg tggtaaaatt attcctcgtc gtatttctgg
     2941 tacaagtgca aaatatcaac gtatgttgaa ccaagctgtt aaacgtgctc gtcaaatggc
     3001 tattttgcct tttgtcgcag atagcattcg ctaaggagat atcatggaag ttattcttaa
     3061 agcaaatgtt gctaagcttg gtaaaatgct cgatatcgtt aaggtaaaag atgggtacgc
     3121 tcgtaacttt cttttcccaa aaaaattagc gatgcgtgca acaaaagctg ctaaacttga
     3181 aattcaacgc aatcgtgaag ctatggaagc tttattccgc aaagaacttg ctgctgcagg
     3241 tgatttggca gctaaacttt cagaagtgac tgtgaactta actcgtcgcg tcgtagaaga
     3301 cgaacgtctt tatggttcag tgtccgctgc tgacattgca gaagccatta ctttacaagg
     3361 tattaagatc aatcgcaatc aagtgattct tcctgaacca atcaaacaat taggtgtatt
     3421 ttccgtcgct atcaatgtat ttggcgatgt agaagccact gtaaaagttt gggttgttaa
     3481 agaaaattaa acaattggct tttataaaaa aaaccgcttc attttgaggc ggtttttttt
     3541 gtacatttaa tgcatgcatt ataggttatt ttttctactc attattattt tttctacctc
     3601 ttcttttgcc caggttagag atcttttcgc tgaatttgaa gccgaagtta agccctcatc
     3661 ttcctcttca gaagaagttc ctgttttatc tagttcttct atttcagaaa gtcccttgtc
     3721 atcaagctct tcttcaataa aagagatttc ttcctcctca gcagaagttc ctgttttatc
     3781 tagttcttct atttcagaaa accctgtgtc atcaagctct tcacaagtag caccagagac
     3841 tccagttgct gatacatccg tgatttctat accagtatca tcaagttctt cttctgtaaa
     3901 catggtttcc tcttcgtcta tacaagttgt tgctccaatt gcatccagtt cttccatgtc
     3961 tcgtcgtgat ttattggggc cagtgaaggt ttctaaaatc cgtggcatta acgaaatgaa
     4021 gggctcgtat aaaagtccta gaaaggcttt atttatgtct ttagttcttc ctggtgcagg
     4081 gcaaatatat gtgggcactt cgacatttaa taaagtgaga ggtggcgttt atctagctct
     4141 agaggctgct ttatggagtg gctggtatta ttactctgtt cataaataca ataatcaagt
     4201 ttcgagatat aaagattatg caaatactca tttctctgtt ggagcgtatg aacaagaaat
     4261 gcataacatc tatttgcttc aattagaaga cgcttctgaa gaggcgaatt ttagaataag
     4321 gtatttaaat agtcgtgaat cttattgtaa atcaatttat ggcacggcta ctacaaataa
     4381 ctgttatagt agtgaaaacc ttttcgagaa agattcaagg catttagatc tttttgctaa
     4441 tgtttctctt ggtgaatcca aaaaaggcac aagcttttat gatgacaatc aattttatca
     4501 gctgattgca aacccaattt ttgtcttggg ttgggatgat gtgactgata gagcctctta
     4561 tcaacagtta ggattatcgg attctcaatc agaaacggtt cctttaggta cttcttctaa
     4621 tttaagtact tatcaagata tgcgtgataa agcaaatgac tatgcgtcta tgcaagcttg
     4681 gttttttggc ggtttaatct taaaccacgt tatttctgct tttgatgcag ctctttctgc
     4741 taatgcaaat aataagttat tgtatgaaga aaaattatct tggttagatc aaatgcatct
     4801 aggtagtagc gtatcctttt ttgatggatt tatggcttct gttcaggcct attggaattt
     4861 ttaatgaaga aaaagatttt ttgttttgtt ttattatgct ctgtttctct ttgggctcaa
     4921 acagttattt ctatagacga atctattagt cgtaatcaaa acaaaagcat tgccttgtca
     4981 ttaggagctt cattattatt gcctggaacg ggagaatatt atcttggtga gaaacagttg
     5041 cttcgtcctt ttatttggac tgatgcagct ttgtggatca ctacgatcgg gtcttacttt
     5101 ataggtgaaa agtatatttc ttctgcgcat aattatgcgg tgactcatgc tggtgtttca
     5161 tctacaaaaa aaagtgttga tttcttaaat accgttgctg attatcgtag tcgtggaggc
     5221 gtttttgggc aaaactcgaa tcccgatatg aatgaagatt ataatcaagc gatgattcga
     5281 tcgggtaaat cagtagatga agagtatcct aatacaatgg agtatcaatg ggattggggc
     5341 tcaagcgatt atccagaaaa tacagaacat atggatatat ataagaatat gcttcggaat
     5401 taccgagtct ctagaattgt atttcaagtt tctgtcggtg ctttgatatt aaatcgtgtg
     5461 gtttcgttct tagatgtcat gcgtatttat cgtgccactt cttctaaaaa aatgactcgt
     5521 cgttgggata ttgcgcctca gttttatcct gacggtagtg gtgctgtttt tagctatcaa
     5581 ttctaaagca ttaataaatt aaaagaagag gggttgtttt accgtggctt cctactctga
     5641 agtgatataa tcctggagac attatacttt ttatatagtc aggttttaaa caatgagtgg
     5701 ttgaatttgt tggaattctt ataactaaat ttcctattct gtttctgatt tctataaaag
     5761 atcttgtatt agggagtgga gaaaagcata gttcagtagt cccttgccac ggtgtagggt
     5821 atgcacggag aggcttagcc acaagaggaa cgattgtagg ggaatcatca ctaaagcgac
     5881 taaggactag tattgctgaa tctgattcag agatggttga tcgccataag tcgatatcgt
     5941 ctgcatttaa gagtgatttt atagatactt ctgaatcatc gctattccag aaaaaaagtg
     6001 atgcttggcc cttaaaatag gaaatatcag gcatactaga tttgatggat aaataattaa
     6061 aagctaagga actcaagttc tggtttttct cgttgtaggg gcgtatggtc atttctggcc
     6121 aatgaggcat gtcttcataa taaaggctat ccgtgggctt gtatttggat cttgtgtttg
     6181 taaagaagag ctgctttgca aaatcatgaa acgcagaatc aggctcaatt ccttgagcga
     6241 cccatttttt ggaaagaatt tcataaaaag gtttttctgg atttttagaa taggcttccc
     6301 agatagaggc atcaaaaagg gggccattgt tttcgtataa gtataagtag aaaacgactg
     6361 cactataatc ttgatttaag gccgataacg ctgtaccagg ataatcaaat atatcataga
     6421 ggtaacccca ataatcattc acctcttttg ccccaatttc ttctactcct gttgctgacg
     6481 cttcaaacca aaatgaagga tagtcgataa aactcatgta acgaatttgt gcggcatgat
     6541 aaagttcgtg aaatgcagtg acttgaatcg ctttttcagg ataatcaaag taagaataag
     6601 atgtttgatt attattgtaa agttctttag ttgcgattgg ataactacat tctttacctt
     6661 ttgaatctgt aatccaagct tcttgttggt tgtattctgg agtatattta aaatcattat
     6721 caatcatcaa gatggtggct tcagggtttt ctggatcacc agggtaggtg agcccaaaac
     6781 aaccatggca agaccctccc attaaattat ctgtatcgcg taaaaggtct atgtctatta
     6841 cttcaacggg gtaaagcccc aatggatttt cttgtttata gtgataggag cggctcattg
     6901 cttttggagg cttcatccct aatttagtgg tgtgtaaatt ccatgctttt tctaaataaa
     6961 aagtgagaga atctaaaaac gattcagtgg tcgcgtgtgg gccttctagg gtgtaataaa
     7021 tgacaaaatg tttagttaaa atggtatcga ttttatcgta ataggattct tcaggacagg
     7081 aggtcaaagc agactgagcg atggatctag aaagaagtcc agttttaatt tgtttttggt
     7141 gtttgataaa tttttcagta ccacaagtgg ggcgaaggcc aaaagaccat gaaaataaaa
     7201 ggaaaagaac aaaaaataaa aagcgcatct taagctcttt ttaatgcttt taagagtttt
     7261 tgttctgcac ggtcttctac ttcaaaattg gtttctagcg gcctttgatg atgagctttt
     7321 atacgtgcgt caataagaat aggggcttca catgcaaaat gtttgtagtg aatgtgatgg
     7381 cgaacgccat aaatatcttg agcggggtct gaacgcgtga aggtgatcca gagccagttt
     7441 tcaatgcttt ccgatgtaaa tgaagcgtcg ttcacaatgc taatccaagg gtatagttct
     7501 ttatgttccc atgattctaa agctctggcg agagcttcca tggaggctct cgatttcgct
     7561 tctagagcta atacaccagg catgacgtat tttaaattag aaaagccttc tactttagga
     7621 aatgtttgga atacttcccc ttgagcattt ccgagttctc gttttggttt tccagcagcg
     7681 gctaagacta atttagaccc atgatttaag gatgttcctg aataatctaa ggtatcaatt
     7741 gtggtagatg tttggaaatg tagatctctt gaaaagtcga gtcgagacaa cacgtggcta
     7801 aaaaatgcag gaatattttt tgtagttaaa gacggatcgt cttcacaagc cgctatgaga
     7861 aggtatttag ctagagcggc ttgattaaat ccaagaagtg cattggatgt ttttaataat
     7921 tccatcggtt ccgaagggcg ataaggaaca tagcgttcac tgccgatcgc aaggagtaga
     7981 ggatgaactc ctgcggcatc tacggcatgc atttccataa gcccaggaat agatgctggt
     8041 accatcgggg cagtaatttt atgaattaaa tgcccaaaaa cggtgtcttc ttgaggaggc
     8101 cttcctacaa cagtaaatgg gaaaatggca ttttttttat ggaatacttt tgaaactttt
     8161 aagaatggaa aattatgttt tgcagagtaa tagcctaagt ggtctccgaa aggtccctcg
     8221 ggcttttgaa gaatatcgat ttcccctaaa atgcagaaat ctgcatctgc tgagataacc
     8281 cagccttcgt gcatggaata cctaaacgcg cgaccgttca acaggcctgc cataattagc
     8341 tcggataaat tctcgggcat aggcatcact gctgcaagcg tatgtgcagg aggcccaccg
     8401 ataaaaatgc tcacttttaa tggttttttt agaatgattg ctttttgatg atggcgtgca
     8461 atgtctcggt taatctgata atgtaaacca cattcttgat ttgcgatgta atcatttcct
     8521 gaaatttgaa ttcgatacat tcctagattt gcttttagaa gagaggattc ttctggatct
     8581 aaagaacata cttgaggtag cgtgataaaa gctcctccat cttctttcca agagtgtagt
     8641 tgaggaagat cgcttaaaga acattcttct ttaaggacgg gtacttgaaa agaacgttgt
     8701 ggctttgctt ggatggcggc aaaaggcagt aaaggcaaat tctgtggcgc ttgaaataca
     8761 gaaaggggat ttgcttttgc tgatattgcc gtttcaagag ttttccattc tttgcgaaat
     8821 aaaaattcag ctcgttcaag cgttccaaaa atattacagg ctgcgcgaaa aggggagcct
     8881 tttacttttt caaaaagaat cgctggcgct tttcttttat aagcttgtct tgctatagaa
     8941 gccatttcta aatatggatc gacctcttca gaaatggtaa gaagcattcc cgcttttttg
     9001 agatcaatta aagcatcttc taaagactta tacgacataa taaaatgtta tgactaagat
     9061 agtctactta aaatatatta accagcagtc gaagaaccaa aacaatcgag agattcaaaa
     9121 gatttggttt ttttcttaga ctcgtatgcc attaacattt taagtgtttt ctttcgttct
     9181 tctaaaccat acagaatgcg tgctttttca atttctgtac gaaaagagtc ttcttcccct
     9241 tgaaaagaga gctcttcata gcagacggaa agatgaccgt catttaaaat agagtgttta
     9301 gcattaaagc cacgaaatgg cccttttcca aggcgagtgg taatctgatt gttttgagaa
     9361 gaactaataa taccagtcca agaaattcga ttaaaaccag ccactaagtg gtaatgaaca
     9421 gtggctcctg tggcggtccc ttcgggcatg tgataatgaa cgattggggt actttccatt
     9481 tttagatgaa acggagcaat ttgaagagta tttataagct cttcctttga tgtttcaaac
     9541 caggctgttt gagaaaaagt taccatatat tgtataaaga tactttcata aatacaattt
     9601 gtccacattt tttcaaatct tattggaaat tctaccatat ttttaattcg tttgatgtag
     9661 tagattatct gcttctttta taatcaaaaa aaatgaaaat tcaattagct cggatatctc
     9721 gcaaaaaatc ttttttactt tggcgtatta atttagagag tgttttcagt tgtatgcttt
     9781 agtagacatt tcattaaaaa tgactaaaaa tttttattaa aaagcgaacg aattggtgca
     9841 aaagaagctc cattctgatg atagtgagtc ataagcccaa atttctttgc tccttctata
     9901 tcttttttat aatcatctcc gaccattaac gtagaggaag ctgagaggtt tagcttttga
     9961 agtgtgtatt cgaaaatttc agcatcaggc tttttaaggc ctacttcact tgaaattaca
    10021 atagcatcca ttaaatcaga gagcttcgtt tgtttcaaaa aacgataaac atgagggtaa
    10081 tgatcaaagt ttgaaagaat agctgttttt acgttggctc tttttaattc atcaagaaag
    10141 ggaatggctt caggatgtaa aaccatttcc ttttgccaag aatccatgga acgatttgaa
    10201 aaatcacata tccaatctaa agaagggttc gctcctattt tcttaaatag aaatagtaac
    10261 cgttcttcat agggggtgat attaaaaagg cgagtcacgg ggaaagagaa gaaggtttca
    10321 cattcctttg ccatttgctc tgcactggtc gaaaaaccga gttcttttgc ccctaatgct
    10381 aaagattcat gccatgaaaa aaacgcctta cccatatttt tatagataaa tagcgttcca
    10441 taaaggtcaa aaataacgcc acgtgggggc atttaaatct cgaagtctac cacgcaacga
    10501 tcctctgtta ccgctctaat aaaggtcgct accttttgat gctctgggtg gatctgatag
    10561 ttatttagat cttctgtgtt ttggaaagaa gagtataaag cgagatccca cgcagcacca
    10621 gaacggttaa aatcgatacc tgcttcaata gaaattaaag aaggaatctt cccttttaaa
    10681 tcgtgtaaca tgtccactat cttggttgcg ttttctattg cagaagcacc ttctgcatta
    10741 tctttcattt tccataaaac gatatgtttg atcatgattt cctcgatgat ataatagtag
    10801 agtatatttt taattgattt gaaaagcaat tatcccaact gaatttttca gcataaagcc
    10861 tagctgcttt ttgcttttga gtaagatctt gttcacaaag ttctaatagg catttcgcaa
    10921 gagcgtctgc tttataggat tctaaaataa gccctgctcc agaagaagaa acgtgttctc
    10981 ttgctgcacc agctgaagca ccaatgagtg cattgccgca agccatgctt tctaaaatag
    11041 aaaggccgaa ggtttcccaa gcagaaaggg cgacgcctat atttgcactt gcataatagc
    11101 gggccatttc ttcttcagaa gataagaaac ctacataatg aagatgttta tattttttta
    11161 gagccacatc aattaaaagt tgaagagggc ctgtgccagc gaaaacaacc gcagggacag
    11221 ggttcatttt ttctgctaaa atggaatagg cttttaaaaa gaggtcaaga cctttctctt
    11281 tagaaaaacg atgaggaaaa aagataatag atcgtttttc gtcgccttgt tttaattctt
    11341 ttactaaagc ttcatctctt ttgttaggag aaaaaagctc aatattacag cctaaaggaa
    11401 tccaaaataa gtttttaaat tgttcacttt caagcctttc gattatctct ttacaagaaa
    11461 cttgaatccc gtgatagttt ttgaattgat aatgtgcata gagaaaagcg agttttctag
    11521 cgaaggcacc tatctttttc ccaaaaaagc gagtaaaagc tctttgtaca taagtgattg
    11581 gaaaatcggc gtgccaaaat cctaataatt ttgcttgagg gcaaatttgg agggcttgtt
    11641 tagaaacggc ttttggtaag atataaggag agccaacttc aatcacatcg ggattgtatt
    11701 tttttaggta aggtgcaatc tgtttctttt tccagataaa acgatagcca ttataaagag
    11761 gcattttaaa tgcttttaca tgctcaacga taagagacga agatacttct tcggtataag
    11821 ttttactgtc aggcattaca aaaacaagga ggtttttgct ctctgaagtg taatacttca
    11881 ttttttctaa atgataacgc ctaacgccac cgccagaggg gctccaaaaa ttattaaaat
    11941 caataatggt catttttatt cttcattcat tcgattggaa atcgaatcca taataatatt
    12001 accttggtaa agatggtttt taccaagacg tttggaatca acagataaaa ataaatttcc
    12061 accttttgat tcaagccaga aaatggcatc tgaatctctt aagtaaggac gaggccctac
    12121 taagccagat tctgtacaga gaagttctcc accaagaaaa atagggatgt caagcgcttt
    12181 atataaatca agcatcactg tggagcgcct ttcgttaata tcagaaatat catttctatc
    12241 ggtaattttc aggtgatcta ttccatcgat gatgacagct aagtcttggt gctctttaag
    12301 ttcttctttt aaaatacgaa ggatttcgac gttatcaaaa ccaggctcat cttcccagat
    12361 ttcaagccga ttttgacgca cataggagat catttctttt gtggcttcta gtattttttt
    12421 attagtctca tcatttgcgt tttgtttttt tactgttaaa atagattcac ctgtcatcat
    12481 ggctatcata cgatcaaata gttgccgacg aggtgtttca agaccaatat agatgacctt
    12541 gatttttgga ttagattcta ctaagtctaa tgcgagactt gtaagtaaaa ggcttttcaa
    12601 accaaaaggt tgtgatgaaa taaaaaaaca accagattga attccatcaa tcgtggcgtc
    12661 taatttagga aagtttgata acgaatagcc aacactcgtg gttggagggc atgccatcgc
    12721 attatcgaaa ttctctttga tatcttgaag cattacagaa cgacgatgtg tatgaatcgt
    12781 atcttcttcg gtatgtgtaa tgagttctaa aagttgatcc gctccatttt ttcgaactat
    12841 ttgatcgatt gtttcgtctt taggaagaat aatggatttg aatttaagat ctaaattttc
    12901 tgctagtttt aagtatttct ggatttggta ttcttgctga cgccgattgg catcgcgacg
    12961 taaaatcaca gtcacagatt ctgcaccaca ggctttaagc ttatacaaat ggctttgtgt
    13021 caattcttga tacattgttg aaacaacacc tgcaatgcca gctaaatcgg ctgttaacgc
    13081 gtcaaaaaag ccctccacaa tgacaggcct ttcgttatcg gcttggatat taaaaggaat
    13141 atcggctggg ccagaagcat aagaaatata aggatgaggg tggttctctt ccatgcttct
    13201 tccataaaca gaatgaataa tcccttttga gtttcttgca gggatagtta ttctaggttc
    13261 acgatatact tctaaattat caagatagaa gtttatttga tgacgctcta caccagcaag
    13321 catgctgtaa ctaataaaag gttctgggtc tccagaataa taaccgatac cgtataattg
    13381 tgcttgccca atattccagc ctctctttgc caaaaattca gcagaagact ggcttttaca
    13441 agcagcccaa tgacaatagc taacaaactg ttctaaaact tttgattttt cgccaccaat
    13501 ttctttcacc catgggtgtt cgtattcaga tttatctgga tcagcctgct cttgggctcc
    13561 aaagaattcg agtgcttcaa tacgagcggc ttgatcatct aaaccgttaa aacggctacg
    13621 catgataaat tcaacaagat caccttcgct gccacattgt aaacaacgat aacgccaata
    13681 gcctaaagca tcgtaaaaga aaagggacgt ttcttctgga gcgtgaaaag gacaacagga
    13741 aattaaatta cgtccccatc ggctgagggg gattcctaag tgcctaggat gagttttaat
    13801 gctttgaagt ccagtgcgat ctttgtccat aaacatgaaa aagcctcttt ttttccaagt
    13861 atcataaagt gatgctatat attcctcata aaaatctaat aaacaaaaga ctaaagagaa
    13921 tcactaaaaa aatctttttc ttgatatatt gttattatta ggatatggta attctcggaa
    13981 tagacccagg ttcacataca acaggatatg cttttttaga aacggctcct caatggattc
    14041 gtgttttaga gtatggttgt attcgagcct cctctacaaa gtctccagaa atgcgtttat
    14101 tggatataat tacggattta gaagccctta taaaccagta caagccaaca gcattaagca
    14161 tggaaggtat cttctttgca aaaaacataa aaagtgcatt gttgttaggg catgtacgag
    14221 gcgctattct tgccatttgc ttaaaacggg gcatgactta ccatgaatac agcccaaaat
    14281 ctgtaaaatt agcagtgact ggtagcggct cttctagtaa agaacgtgtt tgtcaaatgg
    14341 ttcaagttca cttacagtta aaagaaataa agggcccttt agatgcatca gatgcattag
    14401 ccattgcttg gactcatgcg catcctgtga ccactgtttc taaacctgcg tgtttagtca
    14461 aaaagaaacg gaaaaatcaa gcacaagaat ggcgttcttt actaaaaaag tgtggagggt
    14521 taagtgaatg aagttgtttc aaaattagcc ccatttggcc ttgatgaaga gggtttagtt
    14581 tctctatatc cagaaaagct actcctacga aaagaggctt tcaacgcttt agaacagtta
    14641 aggcagttgg ctagattatt tggctttgat atacgagtag aaagcgctta tcgttctttt
    14701 gatcgccaaa tct
//
LOCUS       DAOOBB010000028        14546 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_5355_length_14546_cov_4.664136, whole genome shotgun sequence.
ACCESSION   DAOOBB010000028 DAOOBB010000000
VERSION     DAOOBB010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 14546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14546
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5355_length_14546_cov_4.664136"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="PLT31_02455"
     CDS             <1..692
                     /locus_tag="PLT31_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW94028.1"
                     /translation="EKGMNSYQNGQYEQAIDFWKTCTDQGIEHPDLFYNLGNAYFRLG
                     RLGFAIFYYESAHRLNPSDKDIIHNLKHAKSLTKDKVKEAEENPILASIYALHHLISL
                     KGELLIVIGLIWLIAAILLFKVISKSPRNKNICIGAIFILSIFLGIITLSAGYKIFVL
                     ETETKGVVTATSADVTSAPHDRSQTLNILSEGTVFKVLSIGNGWVEIQLGEKIKGFVK
                     TTEVGIIDDLL"
     gene            679..1473
                     /locus_tag="PLT31_02460"
     CDS             679..1473
                     /locus_tag="PLT31_02460"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HPW94029.1"
                     /translation="MTFFDSSTVPWIGALASLIAGSATALGALGVFLMPRKNAKLEDA
                     FMSFAAGVMLAAAFFGLILAGIHAAEKIGFSSISSVSIVIAGILLGSVFVWSLNHFIP
                     HEHFEMGKHGLPDTLRLKRIWLFVIAIAIHNIPEGMAVGVSFAGNNMSNGTSLAFGIG
                     LQNIPEGFAVAVALLAVGYNRSFSFVVASITGFLEPLGGLIGVLTLGIAQTFYPIILG
                     FAGGSMLFIISDEIIPETHRKEHATLSTFCLLGGFVIMMFFDVLFG"
     gene            complement(1480..8241)
                     /gene="sprA"
                     /locus_tag="PLT31_02465"
     CDS             complement(1480..8241)
                     /gene="sprA"
                     /locus_tag="PLT31_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HPW94030.1"
                     /translation="MKYTLLITKTRFSSSSLLVLFLLLSFAFSHSADSTIATSVQYIP
                     LPPNTTPLNSLLPTGGIGQQNGALMNYTRQKNYVHDIDISTRELEVKEQYINKFSRDT
                     LELWSAHYPELALYVMDMYDIGLRRLWLNSLIGKPYDGYTPPETGSMLDITIPVNMPA
                     WMKDFGFNKPRLFLQGSMDIRLLGRGEKDNAPGSTADNLWPSPTLDYNPSFMVKGKIG
                     PYVTVEINNVESGLGVKNQVRVVYEESFKNEFEDYILQRIEAGTTSLGLSGTELTGYS
                     ENHQGLFGIKADWKFGDWKVTTIASQDGGSQEEYTINASESTSEFQILDKGFIPYRYY
                     FLNHDARNAYIKSAIVGQMTSSYPATNLKLYKRSSLNTSKDVLEKITVVYKTQSGEVI
                     EKEVERMIEIPASDYVYDSKTGILKINGVSKNTLIAASWSNDGTERKGTTISKGSKVV
                     LIQWDATLSELKDIDKLMLRNVYSVGISDESSSNFILRLKDKSGNIGSFLKSMGIADS
                     ITGQILVNDNSIFKKDASGSYTGEMWLPCLPSKGSIETAKQNCLEPLRWIDSSATLAQ
                     MYTLPVHNLNRYTSRFYFESVGKRRSSTISVRDPNSSYSVSAGSCMDISPGTEKLKAG
                     SEILVRGTDYDVNYELGQIELLSDRALDPNKQIKVTFECEPLFEIDSKVLLGARAELP
                     LNQYGFSEGSIFGITALYKSQNTTATIPTLGNEPYSSFLWGFNLRLLDSAAWLDSLVQ
                     KVPLLDAKAPSSWRFEAEYASSYHNANTSDNNSALVEDFENSTSGLIYPMSRTLWFPA
                     SPPGGVAENVSTYIEHQDYRHQGEFIWHSNNTELYKKIYPNVGNSEVDNQHLSVLKMT
                     LRPNDNLVGKSWGGIMRPNSSYYEDLSQKKYIEIVARGNVGSIYLDLGLISEDISING
                     ASPNKTYDGENDLGFTTALHDKGLDGLSGNEEKRIVWDCRVSGCEWSYANPEPFDTDI
                     AKDNFNPNLDDDSDPPVSINGTEGNAGERSYDSEDINRNGALDVDISFVRYRIDLSSN
                     DSTLFETLKNGWRRWRIPLSQYDTIVSQSGNNYNTILSESQFTRLWLGNLNPGVSEAK
                     VQIVQLGVMGNYWEETDASEHYMTSSTEQSQVAEINGSETEVSASVNIKDTAFIDVST
                     INNRENADVYFKSPNSATERDVSTNAVLKETALVLKYKNLSPGQEVGVTRVFDNGVKD
                     FTSYKSIKMEIHYETKAATVPIRFALQFGEGALEGSNNYYEWSFKPVNYNCVSARVQD
                     CHEENWLDNAFAMSLSAFSDLKKGRRPPYLVPVEKNLGGERDEKIKLVGNPSTSSVDW
                     IRFVIIADEGASTSDLNGTFWVNDLRLSEMDADWGNAIRLSGQVNVSDFMTLSGALRY
                     QDGDFATLSTTGGSPKPSLSVAASQLDVAGDVSFNLNKFFNEEHGLRIPMSFGYSSTT
                     TRPYLKPTDDLQLSRDNIVDISSDFIQNKLTMDYEEEQAKRDAAEAKGYQSYITNKNF
                     SLSYSKEYKPDEGRAAEILSQVFLERPVWSYSYNETESKTSINADSTYSYRTMLEYRL
                     GTFNRFNYKPFKSLQNKNWAKPFVDMSFEPWPQTFDLTLIDFSYVRYVDQDRHADYVE
                     PQSDKVVTYTADLNHKANIRWNILPFLSTSYSVNIKRDMYGGGDREDFTAENLFSFDE
                     GGLFAMDRIFDYDHSDRRVYVSRDSVIAIPIDTIATVTPTGDTMTIDLQNQSSYKILY
                     DSTYFYKVDSVGQRHYGKAYGILRNERARNQQFRVSLTPSFISFLPFSTSFTSDFSQQ
                     KTIPDQFSLFDETALEKNFWTISQTNRFELAPTFKLIEFAKLFGKENSVAKLLEKLKW
                     REFRTNWTVNLNTIGENFTLAQLYEDQGVNAFQYYLYGLGLGNGYKNRGLWNIISGDM
                     ALDSRDDYRNFAQYLNQNVDTIVYQGNFKHAVSRAFQIASGLTLPFWDIVLTGDLQWK
                     QDFAQSREYPLYIDSTVIWPKIGVGMTIPNFAKRLSFLSSFKSVSTSHRMDYSYMTTV
                     KPFQSAEDSWTTIWNFNPLVRLSFLLNNNIRIDNSVRLKLEYSDRRPKEEVISQTNWP
                     AEQENIQDTSSYFLNTPWMHTSLYKDYGYNVANDLTITYPFKTKRGFQLWKWYIKLKN
                     DIDIKLTAGYEYKKTIRELYDTEDDFNMTNKESGTDGVFREWNIDGKPYVVYKPSLSL
                     TDRTVPLRTHEWFIRPNVAYQFNKMASASAYIEYRQIHEKLDDETPHLKQILMFEIAL
                     MLKFN"
     gene            8316..8912
                     /locus_tag="PLT31_02470"
     CDS             8316..8912
                     /locus_tag="PLT31_02470"
                     /inference="COORDINATES: protein motif:HMM:NF015423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HPW94031.1"
                     /translation="MLVLIDLLGTLAFAISGASKAIYYKLDWLGLLVMAIVTGVGGGI
                     TRDLLLGSTPPLALQNPNYIMVCIAGAFLTLIVEKRFRFFMKLTLIIDALGLGFFTAV
                     GASKAAQMDSSALTIVLLAMITAAGGGLIRDLLVSEIPQVLRSDFYATAALLGGLLFL
                     ALENTSYSFSTQILITTFFTFVIRIIAIRHKLHLPKTG"
     gene            complement(9030..10907)
                     /locus_tag="PLT31_02475"
     CDS             complement(9030..10907)
                     /locus_tag="PLT31_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIPR family protein"
                     /protein_id="HPW94032.1"
                     /translation="MELTKSQERRLSFVASLLSLNPNDPTDRIKALRHLNLDEEGCFH
                     GFQYSQGFLELFIFLDEMTPLEKVVCNLNQDLKQLQIAVFRTSDPENPFFMSLREEAD
                     PWAVLDVNSTEDDDEDAPASRPYMETLEITVLRTRYSRDITDSEMERTLKDLRRKLNL
                     WKIDVRAKVEIIAEHDDLTRDFRSADDKLEIEMDAAPLHMTSDRGELFLGFCPIRTIF
                     EYHLNLGKRLKTKHNMAIVSSNIRTYLGNLTHTNAALIDAFQEMERSNDSTDFPFLHN
                     GMTLTGDDLRLKDRDGKKVLLIERPKIINGAQSLFTYEQYAKKSKKPVNPQVLVKVVV
                     PYAGSDSFLRQVTMANNRQNPVFSYHLRAADDLQFFIWQRYQEEGFTYVYKDGVRLKA
                     RTRKLEVRMRPELAKTLFMMDAKISESKSSDCIFDKESLYQRCFGAFLRVSPQKEKDF
                     VRKTIAYTKAWQLLTRLPIKIRNVVPGQGVSIEANDDNPLTKITWNGRLEDKFVFRSA
                     VRDLVVSIALRHWLIYGDPIQDFNYLVDNELDFNNSILRYASKIYSDHLKGILVSEFK
                     DDKNYYDTITTIDKDSGESVERRLWKGFGNTQTYDKVMNMLVKKDPSWAKCRGGIEAF
                     I"
     gene            11192..11944
                     /locus_tag="PLT31_02480"
     CDS             11192..11944
                     /locus_tag="PLT31_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HPW94033.1"
                     /translation="MNLLDCFAEIKAKKATKLDLSNQHLRVLPPELFTLDFLEELILD
                     ENLLVEIPDDLCKLKNLKSLSVSENDLMVLPDSIGELSQLENLYLGYNSLSELPESIT
                     KLTHLKKVNIAKNQLLDLPKEIGQWAEVQKLSLHDNMLSEIPESIGKMKNLEKLYLDN
                     NDLNALPSSIGGIKKLEILMVSGNNLTFLPKEIGLLSQLRELVLDANQLDSVPESLAD
                     CKKLEFLSLVENPMVESLPKILVSNSGLRIES"
     gene            11993..13216
                     /locus_tag="PLT31_02485"
     CDS             11993..13216
                     /locus_tag="PLT31_02485"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPW94034.1"
                     /translation="MTFTIPSCILDNPVSEVPDPSEFEYNYWLVSHWYLHSEEIKEAS
                     FYEGNTTKLYNSLSDPYTQYIPPSISDEAEQQINTSIVAGDIGVEILLNQQSEYPLFI
                     YRIYPSSPASNANIARYSTIISVNGIELKGSNAYTTYQQELATHKDIILTVSKNNVID
                     TFSLTKENIIIPTIFLDTLNGVLFISIETFKPYTWDQVNGSLGEMRSILEKTKNASLR
                     VLDLRNNLGGHLNQCIGMADLFVSEGILSSRSYKQINADGKISLFNSTTVATSGDPGE
                     QGEFLLFVNRNTASCAEIFTAALAQNRSFTVVGETTYGKGVGQNTWKTKDNGLAIITN
                     MIFKTPNGSIYNTQGIEPDVVCENANYDCIFNLIQKKSLLKSNNINSVYFDSFFRPSL
                     YEPFSFGGAIEYILE"
     gene            13227..>14546
                     /locus_tag="PLT31_02490"
     CDS             13227..>14546
                     /locus_tag="PLT31_02490"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPW94035.1"
                     /translation="MKTKVFISSSFFLFMVLFFSLLFMSCTQEYKPLNPNNQEPQNRN
                     DSIAYEFEWNYLLLKGFYFYADQELYGIDYYDGLLKSNDPYAKIYAMYEEMSDPFTQY
                     FSPEYFDMVMNALIYSESTSGLGFEINKDLKVTQVYENGPAEKEGLQRGDLVLMVDSN
                     SFSSKEIFDRLVEGEVDDEREIIVKRDSDTLSFTIAIDYISMPTVFLDSLDSIPLIKI
                     TEFTDTTSNVGGTYAEFKNALIKTDGAAATIIDLRGNPGGSILHCTKMAAEFLSKNDT
                     VINEISKDLDSTTFEQVTDTAVYIAQKDGLGKGRYYVFLQDEGSASCSEILIAGVTTN
                     LKSPVIGTLSYGKGIGQYYFITYLSGIAGVTSFNFYDKSFETYNHRGIMPDFVFESDS
                     LILAKAAELAKAKTYKRTKKYGTTYKAFEKKASNSQKGTAQNKPGAFK"
BASE COUNT         4668 a         2859 c         2369 g         4650 t
ORIGIN      
        1 cagagaaagg aatgaattcc tatcaaaacg gtcaatacga gcaagccatt gatttttgga
       61 aaacatgtac tgaccaaggc atagaacatc cagatctatt ttataactta ggtaacgctt
      121 attttagact cggacgtctt ggctttgcca tattctatta tgaatcagcg catcgcttaa
      181 atcctagcga taaagacatt atacacaatc taaaacacgc aaaatcgtta accaaggata
      241 aagtaaaaga agcagaagaa aatccaatcc ttgcttccat ttatgccctt catcatttaa
      301 tttccttaaa aggagaatta ttgatcgtga tagggttaat atggcttatt gcagccattc
      361 tccttttcaa agtcatcagt aaatcacctc gaaataaaaa tatttgcatt ggcgctattt
      421 ttattttatc cattttctta ggaatcatta ctttaagcgc aggctataag atttttgttc
      481 tagaaacaga aaccaaaggt gtcgttactg ccaccagtgc agatgtcaca agcgcccctc
      541 atgaccgaag tcaaacgctc aatatcctat ccgaagggac tgtttttaag gttctttcta
      601 ttggaaacgg ttgggtagaa atacaactag gtgaaaagat taaaggcttt gtaaaaacaa
      661 ctgaagtagg aataattgat gaccttcttt gattcttcta ctgtcccatg gataggagct
      721 ttagcaagtt taattgcagg gagtgcaact gcattgggtg ctttaggcgt ttttttaatg
      781 cctagaaaaa atgcaaagct tgaagatgcc ttcatgagtt ttgctgcagg agtaatgctt
      841 gccgccgcct tttttggttt aatccttgca gggattcatg ccgctgaaaa aattggattt
      901 tcgtcaattt caagtgttag cattgtgatt gcgggcatcc ttctcgggtc tgtttttgtt
      961 tggtcattaa atcacttcat tcctcacgaa cactttgaaa tggggaaaca tggcttaccc
     1021 gatacattac gccttaaaag aatatggctt tttgtaattg cgattgctat tcataacatt
     1081 cctgaaggaa tggctgtagg agtgagcttt gcagggaaca acatgtctaa tggaacgtct
     1141 cttgcttttg gtattgggct ccaaaacatc cctgaaggat tcgcagtcgc tgtcgcactt
     1201 cttgctgttg gatacaatcg ttcattttca tttgttgtag cttccattac aggttttcta
     1261 gagcccttag gaggtcttat aggagtactc actttaggga tagctcaaac gttttatcca
     1321 ataattcttg gatttgctgg tggtagcatg ttatttatta tttctgatga aattattcct
     1381 gaaacgcatc gaaaagaaca cgccaccctt tcaacgtttt gtttattagg aggctttgtc
     1441 ataatgatgt tttttgatgt tttatttggt taactttttc taattgaatt tcaacatcag
     1501 agctatttca aacattaata tctgtttcaa atgaggggtt tcgtcatcta acttttcatg
     1561 aatttgacga tattcgatat aagcactcgc agaggccatt ttattaaact gatacgccac
     1621 atttggacga ataaaccatt catgtgttct taaaggaacc gtacgatcag tcaatgaaag
     1681 agaaggttta tataccacgt atggtttacc atctatattc cactccctaa acactccatc
     1741 cgtaccactt tctttatttg tcatattaaa gtcgtcttct gtatcatata attcacgtat
     1801 ggtcttctta tactcataac ccgccgtgag cttaatatca atatcatttt ttaatttaat
     1861 ataccatttc cacaattgaa atccgcgttt tgttttaaag ggataagtaa tcgttaagtc
     1921 attagccacg ttatagccat aatctttata taaagaagta tgcatccatg gcgtattcaa
     1981 gaaataagaa gaagtatctt gaatattctc ttgctctgca ggccaattcg tttggcttat
     2041 tacttcctct ttaggacgtc tatctgagta ttctaatttt aagcgcacac tattatcaat
     2101 acgaatatta ttatttaata aaaatgaaag ccgaactaaa ggattgaaat tccagattgt
     2161 ggtccatgaa tcttctgcac tctggaaagg cttcacggtg gtcatataag aataatccat
     2221 gcgatgactt gttgaaacac ttttaaagct actcaagaaa ctaagtcgtt ttgcaaaatt
     2281 aggaatagtc attcccacac caatttttgg ccaaataaca gtgctatcaa tatacagagg
     2341 atattctcta gattgagcaa aatcctgttt ccactgcaag tcacccgtta aaacgatatc
     2401 ccaaaatggg agagttaaac ctgaagcaat ctggaaagcc ctagacacag catgcttaaa
     2461 gtttccttga tatacaatcg tatctacatt ctggtttaga tactgtgcaa aatttctata
     2521 atcatctcta gaatctaatg ccatatcacc tgaaataatg ttccataagc ctctattttt
     2581 atagccgtta cccaaaccta atccatacaa ataatattga aatgcattta caccttgatc
     2641 ttcgtataat tgagcgagtg taaaattttc acctattgta ttcaaattta cagtccagtt
     2701 tgttctaaac tctctccact ttaatttttc aaggagtttg gcgacactat tttcttttcc
     2761 aaataatttg gcaaactcaa tcaatttaaa tgtcggagca agctcaaatc gatttgtttg
     2821 tgaaatagtc caaaagttct tttctaaagc cgtttcatca aacagactaa attgatctgg
     2881 aattgttttc tgttggctaa aatcacttgt gaatgacgtt gagaatggta aaaatgaaat
     2941 aaatgaagga gttaaagaca ctctaaactg ctgatttctt gctcgttcat ttcttaaaat
     3001 tccataagcc ttaccataat gacgctgccc aacactatcg accttataaa aataggtgct
     3061 atcatataaa atcttataag agctttgatt ctgtaaatca atggtcattg tgtctcccgt
     3121 tggagtcact gtcgcaattg tatcaatagg aatggcaatc acactatctc tagacacata
     3181 aacacgacga tcgctatgat cataatcaaa aatacgatcc atcgcaaata aaccgccttc
     3241 gtcaaaacta aataggtttt cagctgtaaa atcttcccga tctccaccgc catacatatc
     3301 acgtttaata ttaacactat aacttgtaga taagaatggt aaaatattcc aacgaatatt
     3361 tgccttatga tttaaatcag cagtgtatgt taccacctta tcagattgag gttctacata
     3421 atcagcatga cgatcttgat ctacatagcg gacataacta aaatcaatta gagttaaatc
     3481 aaatgtctgt ggccatggtt caaaagacat gtcaacaaat ggtttcgccc aattcttatt
     3541 ttgcaacgat ttaaatggct tatagttaaa acgattaaat gtacctaacc tatattcaag
     3601 catggtacga tatgaatagg tagaatcagc attgattgac gtctttgatt cggtttcgtt
     3661 ataagaataa ctccaaactg gtcgttctaa gaatacttga gaaagaattt ctgcagcacg
     3721 cccttcatct ggtttatatt ctttgctata gcttaagctg aaattcttat tcgtaatata
     3781 agactgatac cctttagcct cggcagcatc tctttttgcc tgttcttctt cataatccat
     3841 agtcagttta ttttgaatga aatctgaaga tatatcaaca atgttatcgc gacttaattg
     3901 taaatcatct gttggcttta aatagggacg ggtcgttgtg ctactataac caaaactcat
     3961 cggtattcga agaccatgtt cttcattaaa gaacttattt agattaaagc ttacatcccc
     4021 agcaacatct aattgagaag ccgcaacgga aagcgaaggc ttaggagaac cacctgttgt
     4081 tgaaagagtt gcaaaatctc catcttgata gcgtaaagct cctgaaaggg tcataaaatc
     4141 agagacattc acttgaccag acaaacgaat cgcatttccc caatccgcgt ccatttcaga
     4201 aagcctcaaa tcattcaccc aaaatgttcc gttcaaatca gaagtagaag caccttcatc
     4261 agcaataatc acaaatcgaa tccaatctac agaacttgtc gaaggattac caacaagttt
     4321 gattttttca tctctttcac cgccaagatt tttttcaact ggcaccaaat aaggtgggcg
     4381 tcgtcctttc ttaagatcag aaaaagcgga gagagacatg gcaaaagcat tgtcaagcca
     4441 gttttcttca tgacaatctt gtactcgtgc agaaacacaa ttgtaattaa ctggtttaaa
     4501 actccattca taataattat tcgaaccttc taaagcacct tcaccaaatt gcaaagcgaa
     4561 acgaatagga actgtagccg ctttcgtttc ataatgaatt tccattttaa tggatttata
     4621 agatgtaaaa tcttttactc cattatcaaa aacgcgagtg actcccactt cttgaccagg
     4681 agaaaggttt ttatatttga gcactaacgc agtttctttc aataccgcgt tcgtactaac
     4741 atctctttct gtagcagaat ttggcgattt aaaataaaca tcagcattct cacgattgtt
     4801 gattgtggat acatcaataa aagcagtatc cttaatgttt acagacgctg aaacttctgt
     4861 ttcacttcca tttatttcag ccacttgcga ctgttctgta ctagacgtca tataatgttc
     4921 agaagcatct gtttcttccc aataatttcc catcactcct agctgaacaa tttgcacttt
     4981 agcttctgaa acccctggat ttaaattacc aagccataat cttgtaaatt gagattcaga
     5041 taatatcgta ttataattgt taccactttg agaaacaatc gtatcatact gacttaatgg
     5101 aattctccaa cgacgccatc catttttcaa tgtttcaaat agagtagaat cattacttga
     5161 caaatcaatc cgataacgaa caaaactaat atctacatct aaggcgccat tacgattaat
     5221 atcttctgaa tcataagaac gctctccagc atttccttcc gttccattaa tagaaacagg
     5281 aggatcactg tcatcatcta aatttgggtt aaaattatcc tttgctatat cagtatcaaa
     5341 tggctctgga tttgcatagc tccattcaca accagacaca cgacaatccc aaacaatacg
     5401 tttttcctca ttaccagaaa ggccatctaa tcctttatca tgaagagccg ttgtaaagcc
     5461 taaatcattt tcgccatcgt aggtcttatt aggtgaggca ccattgatag agatatcttc
     5521 actaatcaga ccaagatcca aataaatgga acccacattt cctctggcaa cgatttcaat
     5581 atattttttt tgacttaagt cttcgtaata actactgtta ggacgcataa taccgcccca
     5641 acttttacca actaaattat cattaggcct aagagtcatt tttaagacag ataaatgctg
     5701 attatcaact tctgaattac caacatttgg ataaatcttt ttatacaatt ctgtattatt
     5761 ggaatgccaa ataaactctc cctgatgcct atagtcttga tgttcaatat aagtactgac
     5821 attttcagct acgcctccag gaggagaagc tgggaaccaa agtgttcgag acattgggta
     5881 aattaaacca gaagtgctat tttcaaaatc ttccactagc gcagaattat tatcgctcgt
     5941 attggcatta tggtagctgc ttgcatattc tgcttcaaac cgccagctcg aaggagcttt
     6001 tgcgtctaac aaaggaacct tttgaactaa agaatctaac caggcggcag aatccaacaa
     6061 tctcaagtta aagccccata agaaactaga ataaggttca tttccaagag taggaatcgt
     6121 agctgtagta ttttgactct tataaagtgc tgtaatacca aatatagaac cttcagaaaa
     6181 gccatattga ttcaaaggca actctgctct tgctccaagc aacaccttac tatcaatttc
     6241 aaaaagaggt tcgcattcaa aagtaacctt aatttgtttg ttcggatcga gagctcgatc
     6301 acttaataat tcaatttgtc ccaattcata attcacatca taatcagtac cgcgcactaa
     6361 aatttctgaa ccagctttta acttttctgt ccctggagag atatccatac aagagcccgc
     6421 actcaccgaa taactggaat taggatcccg aacactgatg gtagagcttc ttctttttcc
     6481 gacagactca aaataaaaac ggctggtgta acgatttaga ttatgaacag gtaaggtata
     6541 catctgagcg agggttgcag aagaatcaat ccatctaagc ggttccaaac agttttgttt
     6601 tgctgtttca atcgatcctt tacttggtaa gcaaggcaac cacatttcac ccgtatacga
     6661 tcccgatgca tctttcttaa atatggagtt atcattgact aaaatctgcc ctgtaataga
     6721 atcagcaata cccatgcttt tcaaaaaaga accaatattt cctgatttat cttttagtct
     6781 tagaataaag ttactggaac tttcatctga aattccaaca gaataaacat ttctaagcat
     6841 taacttatct atatctttta attcagaaag agttgcatcc cattgaatta aaaccacttt
     6901 tgaaccttta gaaatagtag tacccttacg ttcagttcca tcattactcc agctcgcggc
     6961 tattagagta ttttttgaaa caccattgat tttaagaatt cccgtttttg agtcataaac
     7021 ataatcactc gcaggaatct caatcatacg ttctacttct ttttcaatta cttctccaga
     7081 ttgcgtttta taaactactg tgattttttc taagacatct ttacttgtat taagagaact
     7141 tcgtttgtaa agtttcaaat tggttgcggg gtaagaagaa gtcatttggc ctacaattgc
     7201 agatttgatg taagcgttac gcgcatcgtg atttaagaaa taataacgat aaggaataaa
     7261 tcctttatct agaatttgga attccgaagt gctttcactt gcattaatcg tatactcctc
     7321 ttggcttcca ccatcttgag acgcaatagt cgttaccttc cagtccccaa atttccagtc
     7381 cgccttaata ccgaaaagcc cttgatgatt ttcagaatac cctgttaact ctgttccact
     7441 taaacctaga gaggtagtac cagcctcaat acgttgcaat atatagtcct caaactcatt
     7501 tttaaaggac tcttcataaa caacacgtac ttgatttttt acgccaagac cactttccac
     7561 attgttaatt tcaacggtga cataagggcc tatctttccc ttcaccataa agcttggatt
     7621 ataatctaat gtcggagaag gccacaaatt atcggccgtg ctaccaggag cattgtcttt
     7681 ttctccacgc cccaaaagac gaatatccat tgagccttgt aaaaataaac gaggcttatt
     7741 aaaaccaaaa tctttcatcc acgcaggcat attgacaggg atcgtgatat cgagcattga
     7801 ccctgtttct ggaggagtat aaccgtcata aggtttccct attaaagaat tcaaccaaag
     7861 acgtcttaag cctatatcgt acatatccat tacatacaat gctaattcag gataatgagc
     7921 agaccacaac tcgagagtat ctcgactaaa cttatttata tactgttctt taacctctag
     7981 ttctcgagtg gatatatcaa tatcatgaac ataattcttt tggcgagtat aattcattaa
     8041 cgcgccattt tgctggccaa tccctcctgt tggcaataga ctatttaagg gggttgtatt
     8101 tggaggaaga ggaatatact gaacagacgt tgcaatcgta gagtcagccg aatgcgaaaa
     8161 agcaaaagac aacagcaaaa aaaggacaag caatgaactt gaactaaagc gagtttttgt
     8221 aataagcaga gtatatttca aaattatgca ccaaaataca gaattttaca agaaaaaact
     8281 atcttcaaga agagaatagt aataaatttt gtggtatgct tgtactcatt gaccttttag
     8341 gaacgcttgc ctttgctatt tctggagctt caaaagctat ttactataaa ttggactggc
     8401 tagggctgct tgtaatggct attgtcacag gagtgggtgg aggtattact cgagaccttt
     8461 tactcggttc tacacctcct ttagcccttc aaaatccaaa ttacattatg gtttgcattg
     8521 caggtgcatt tctaaccctc atcgtagaaa aaagatttcg cttttttatg aagctaactt
     8581 tgattattga tgctttaggg ctaggctttt ttaccgctgt cggtgcaagt aaggccgctc
     8641 aaatggactc tagtgctctc accattgttc ttctcgcaat gattactgct gcaggtggtg
     8701 gcttaatccg agatctcctt gtaagcgaga taccacaagt tttaagaagt gatttttatg
     8761 ctacggcagc cctacttgga ggcctccttt tcttagcctt agaaaacacc tcttattctt
     8821 tttctacaca aatactcatc actacattct ttacttttgt cattcggatc atagccatta
     8881 ggcataaact tcatcttcct aaaactggct aaaaatattt tcaaatgttt ttaagcatta
     8941 aaacaaaata aagactcaac aaaaaaaccg actcttaata agtcggtttt ttctttttag
     9001 caaagttcta aaaaactatt aaaggagcct taaataaaag cttctatacc accacggcat
     9061 tttgcccaag aaggatcctt tttaacaagc atattcatga ccttatcata agtctgagta
     9121 ttaccgaatc ctttccacaa gcgacgttca acagattcgc ctgaatcctt atcaatcgtc
     9181 gtaatggtat cataatagtt cttatcatcc ttaaattctg aaacgagaat acccttcaag
     9241 tgatcagaat aaattttaga ggcataacgc aaaatggaat tattaaagtc caattcatta
     9301 tccactaaat agttaaaatc ttgtattgga tcaccataaa tcaaccagtg acgtaaagca
     9361 atcgatacca ctaaatcacg aacagcactt ctaaagacaa acttatcttc taagcgacca
     9421 ttccatgtga tctttgtaag tggattatcg tcattggctt caatagaaac accttgccca
     9481 ggaaccacat ttctgatttt aataggcaaa cgagttaata attgccatgc ctttgtataa
     9541 gcaatggttt tccgaacaaa atctttttct ttttgaggag agacacgcaa aaaagcacca
     9601 aaacaacgtt ggtataagct ttccttatca aaaatacaat cagaagattt actttctgag
     9661 atttttgcat ccatcataaa aagagtttta gctagttcag gacgcatacg aacttccaac
     9721 ttacgtgttc ttgcttttaa acgaacaccg tctttataaa cgtatgtaaa gccttcttcc
     9781 tgataacgct gccaaataaa aaactgtaag tcatcagcgg ctctaagatg ataactaaat
     9841 actggattct gacggttatt agccattgtg acttgtctta aaaacgaatc tgatccagca
     9901 taagggacaa cgactttcac caaaacttgt ggattaactg gttttttgct ctttttcgca
     9961 tattgttcat atgtaaaaag actttgagca ccgttaataa tttttggacg ttcaatcaaa
    10021 agcacttttt tgccatcacg gtcttttaag cgcaaatcgt ccccagttaa ggtcattccg
    10081 ttatgtaaaa aaggaaagtc agtggaatca tttgaacgtt ccatttcttg gaaggcgtca
    10141 atcaaagcag cattcgtatg agttaaatta cccaaatacg tacgaatatt actagataca
    10201 atggccatat tgtgctttgt ttttaatctc tttccaagat tcaagtgata ttcgaaaata
    10261 gtacgaattg gacaaaatcc taggaataat tcgcctcgat cacttgtcat atgtaatgga
    10321 gcagcgtcca tttcaatttc aagtttatca tctgcagagc ggaaatccct tgttaagtca
    10381 tcatgttcag caataatttc cacttttgca cgaacatcaa ttttccaaag atttaattta
    10441 cgacgtaaat ccttaagagt acgctccatt tcggaatcag taatatcgcg actatatcga
    10501 gtacgaagaa ctgtgatttc gagcgtttcc atatatggac gacttgctgg agcatcctca
    10561 tcgtcatctt ctgttgaatt cacatctaaa acagcccaag gatcagcttc ttctcggagg
    10621 gacataaaaa atggattttc aggatcactc gttctaaata cggcaatctg taattgtttt
    10681 aaatcttgat ttagattaca aaccactttt tccaaggggg tcatttcatc aagaaagatg
    10741 aaaagttcta aaaatccttg tgaatattga aaaccatgaa aacaaccttc ttcatctaaa
    10801 ttgaggtgcc ttaatgcttt aattcgatct gtggggtcat taggatttaa actcaacaaa
    10861 ctagcgacaa aagagagcct gcgttcctgc gactttgtta attccattat ttcctctaac
    10921 caaagttcaa aaaataatac atatataatc ttatatatgt tacatagata ataaaaatat
    10981 ctttaatact gctttttgag tatataaaag atgtttcttt taaaaaacat attcaatttt
    11041 tacaaaacaa gtacactaaa ctggcttttt tttaaaattg ttgatactgc acaaaaaagc
    11101 aatccaaagt atattatctt ttttcaccta atttgacctc tctttttttt aaaaaagcta
    11161 tgattgtaac aaataggcag gagtatataa catgaatctt ctcgattgtt ttgcagaaat
    11221 taaagctaaa aaagcaacta aattagactt atctaatcaa caccttcgtg tacttccccc
    11281 agaattattc actctggatt ttttagaaga acttatttta gacgaaaatt tgctcgttga
    11341 aatccctgat gacctatgca aactgaaaaa tctaaaaagc ctttccgtta gtgaaaatga
    11401 tctaatggtt ttacctgata gtattggtga attatctcaa ttagaaaatc tttatttagg
    11461 atacaacagc ctttctgaac ttcctgaatc cattacaaag ctaactcatc ttaaaaaagt
    11521 caatattgca aaaaatcaac tactcgattt gcctaaagaa attggtcaat gggcagaagt
    11581 tcaaaagcta agcctccatg ataacatgct ttcagaaatt ccagaaagca ttggaaaaat
    11641 gaagaatctt gaaaagcttt atctggataa taatgattta aatgctctac caagttctat
    11701 tggcggaata aaaaaacttg aaattttaat ggtttcaggg aataatctca catttttacc
    11761 aaaagaaata ggtcttttat ctcaattacg agaactcgtt ctagatgcca atcaattaga
    11821 ttctgttcca gaaagcctag ctgattgtaa gaaattagag tttctttctc ttgttgaaaa
    11881 tcctatggta gaaagtcttc caaagattct cgtctctaat tcaggtctcc gaatcgaaag
    11941 ctaaaccgtg aaaacatttt tacgcttaat acgtttttgc ttattatgcg ttgtgacatt
    12001 cacaattcct tcttgtattc tagacaatcc agtttcagaa gtacctgatc cttctgagtt
    12061 tgaatataat tactggctag tttctcattg gtatcttcat agcgaagaaa ttaaagaagc
    12121 ttctttctat gaaggcaata caacgaaact ttacaatagt ctttctgatc cgtatacaca
    12181 atacatccct ccctctatct cagatgaagc ggagcaacaa attaatacaa gtatcgttgc
    12241 aggagatatc ggcgtagaaa tcctcttaaa tcagcaaagc gaataccctc tttttattta
    12301 tagaatctac ccatcttctc cagcatctaa cgcaaacata gctcgctact ccacgatcat
    12361 ttcagttaat ggaattgaac ttaaagggag taatgcctat acaacgtatc aacaagaact
    12421 agccactcat aaagacatta tactcactgt ttcaaaaaac aatgtaattg atactttttc
    12481 tctaacaaaa gaaaacataa tcattcccac tattttttta gacaccctta atggcgttct
    12541 ttttatttct attgaaacat ttaagcccta tacttgggat caagttaatg gctctttagg
    12601 cgaaatgcgt tctattctag agaagaccaa aaacgcttcc ctacgcgttc ttgatctcag
    12661 gaataatcta ggcggtcact taaatcaatg tattggcatg gctgatcttt ttgtttcgga
    12721 aggaattctt tcttctcgct cttataagca aatcaatgct gacggaaaaa tatcattatt
    12781 taatagtacg acagtcgcta cctctggaga cccaggagaa caaggggagt ttctgctttt
    12841 tgtgaaccgt aatacggctt cttgtgcaga aatttttact gccgctcttg ctcaaaaccg
    12901 ttcatttaca gtcgttggag aaacgacata tggaaaaggg gttggtcaaa acacatggaa
    12961 aacaaaagac aatggtctag ctattatcac caatatgatt tttaaaacac ccaatggttc
    13021 aatttataac acccaaggaa tcgaacctga cgtagtttgt gaaaacgcaa actatgattg
    13081 cattttcaac ttgattcaaa aaaaatcttt attaaaatca aataatatta attctgttta
    13141 ttttgactca ttttttagac cgagtttata tgagcccttt tcttttggtg gagcaattga
    13201 atatattttg gaataggagg ttttcaatga aaacaaaagt ctttatttcg tctagtttct
    13261 ttttatttat ggttcttttt tttagtctac tcttcatgag ctgtactcag gaatataagc
    13321 cacttaatcc aaataaccaa gaaccacaaa atagaaatga ttctatcgcc tatgaatttg
    13381 agtggaacta cctcctttta aaaggctttt acttttatgc agatcaagaa ttatacggca
    13441 tagactatta tgatggtctc ttaaagtcaa acgatccata tgcaaaaatt tatgccatgt
    13501 acgaagagat gagtgaccca tttactcagt atttctctcc agagtatttt gacatggtca
    13561 tgaacgcact catctattca gaaagtactt ctgggcttgg atttgagata aataaggatt
    13621 taaaagtaac gcaagtctat gaaaatggcc ctgcagaaaa agaaggcttg caacgaggtg
    13681 atcttgtttt gatggttgat tccaactctt tttcttcaaa agaaattttt gatcggttag
    13741 tagaaggaga agttgatgat gaaagagaaa tcattgtaaa acgtgattcc gatactcttt
    13801 catttactat tgcaatcgat tacatctcta tgccaacagt ctttttagat tctttggata
    13861 gcattccctt aatcaagatt actgaattta cagatactac gtctaatgta ggtggaactt
    13921 atgctgagtt taaaaacgcc ttaattaaaa ccgatggtgc tgctgctacg attatcgatt
    13981 taagaggtaa tcctggaggt tctattcttc attgcaccaa aatggctgca gaattcttga
    14041 gcaaaaatga taccgtcatt aatgaaatat caaaagatct tgactctaca acttttgaac
    14101 aagtgacaga taccgctgtt tatattgcac aaaaagatgg tctaggtaaa ggacgctatt
    14161 atgtatttct tcaagatgaa gggagcgcta gctgttctga aattttaatt gcaggtgtga
    14221 caaccaactt aaagtctccc gttattggaa cgctctccta tggaaaaggc attggccaat
    14281 attacttcat cacctatctt tctggtatag ctggtgtcac ttcatttaat ttttatgata
    14341 aatcttttga aacctacaat caccgcggca ttatgccaga ctttgttttt gaaagtgatt
    14401 ctttaattct tgctaaagcc gcagaactag caaaagcaaa gacctataaa agaacaaaaa
    14461 aatacggaac cacctataaa gcctttgaga aaaaagcctc taattcccaa aaaggaacgg
    14521 cccagaacaa acctggagcc tttaag
//
LOCUS       DAOOBB010000029        14032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_5612_length_14032_cov_4.189740, whole genome shotgun sequence.
ACCESSION   DAOOBB010000029 DAOOBB010000000
VERSION     DAOOBB010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 14032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14032
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5612_length_14032_cov_4.189740"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="PLT31_02495"
     CDS             complement(<1..717)
                     /locus_tag="PLT31_02495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPW94036.1"
                     /translation="MNSFVDTHCHLDTYEEQTQESFETLWAKMEIKPEAMIQVACDPN
                     EFEYAKIMSEKYDFIYAAYGIHPQYVDSYRPEHEELLKSYLDHPKAVACGEFGLDYHY
                     GAESKEAQCLLFEKELKIGLQKRKPLVLHLREAEKDSLSILRNAPIQNHPIHVHCFTG
                     TPAFAEALLNLSSNIYIGFTGIISFKNAENVREAAKIVPLERLLLETDAPYLAPVPFR
                     GKAAHSGMIPLIAEHLAQVKN"
     gene            complement(714..1232)
                     /locus_tag="PLT31_02500"
     CDS             complement(714..1232)
                     /locus_tag="PLT31_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HPW94037.1"
                     /translation="MNSNLVINNSFFYYCYMQHHISKEGFEKFKAEWESLKYIERPAM
                     QKQVSDAAAEGDRSENAAYTFGRMRLREIDRRLRQLDKLLDGAQIVEAKAPVDGSIRF
                     GAKIQLKEEKTQKLKAYSIVGGEEIDPLQGKISMKSPLGTALLGKKTGEKISVQTPRG
                     ELTYVILEVLYP"
     gene            1231..3072
                     /locus_tag="PLT31_02505"
     CDS             1231..3072
                     /locus_tag="PLT31_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014544932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HPW94038.1"
                     /translation="MKNPIVKRKHPDSRSTRIGRVMFNRMFPKRLDALKVAFSVGIGV
                     FIGIAPTIGIAVILTVAVCAALKLPKVPAVVASFIANPFTQFGFFYPVGYMIGKSIVE
                     PKKISFDFLGELERLSFRNFTDIISHLWINAADHLLSFMIGIMLLAFVFGLAFFGAAY
                     FIVSYRKRKHVEVKNAYIRNLVIEDEQLIKEAKQQGKSFHMHIYPFKALRPVRSEDAK
                     DISALPYDVMNRAEAAKMAENLPHSYLRVTRAELELPEISDAYDPKVYLRAKENLDKM
                     IADGVIAHEKKNCLYVYRQVMEGREQYGLVCCVPAKDYFHKIIKKHELTRADKEEDRL
                     RHVLATNANTGPVFLTYRDQGQFDLFGAVRSRKPIYDFVTEADGFGHTVWLIEDEQEI
                     QTICESFNHVPVSYIADGHHRSAAGARASGYRAEQNKNNTGEEEYNRFLAILFPSTQL
                     KIMDYNRVLKTWNGHTPESILKALEPIFNVKKLNDMIHPQQMHQVHMYLQGSWYQCEF
                     KDELLKDLGPVDSLDVSLLQKHVLNPFFGIDDPRTSKDIDFVGGIRGLSELVKRVDSG
                     ECSCAFAMYPTTLDQLMAIADANEIMPPKSTWFEPKLRDGLLVHTLD"
     gene            complement(3069..3914)
                     /locus_tag="PLT31_02510"
     CDS             complement(3069..3914)
                     /locus_tag="PLT31_02510"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24/S26 family peptidase"
                     /protein_id="HPW94039.1"
                     /translation="MNPSEKLLKKLIVKNSQTRVFFIFLVLIVVFTLSILTRFYAIEH
                     IQIKDNSMAPEIKNNERLWICKTPWCKNQIHPNDIVLAQKRIGEVLLRRVIGLPGSEL
                     KISSEGHVKSAYLDFVWKNETAFIDSRHFYIPKKGDTLFLDSLNDVEMDLAISILKQQ
                     NTSFFIKPSVFQGSQELPLNVVGSARIASRPVSVKEIQGLPWQELFLIGLQVQKSQPS
                     VGEVKIKREVFSLKDSSKIESFIVLEDYYYLACNQGARCIDSRELGLFSPSTIIGKKI
                     RLPWN"
     gene            complement(3916..5736)
                     /gene="lepA"
                     /locus_tag="PLT31_02515"
     CDS             complement(3916..5736)
                     /gene="lepA"
                     /locus_tag="PLT31_02515"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014544934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HPW94040.1"
                     /translation="MSKKHNLIRNFSIIAHIDHGKSTLADRMIEMTHTVASNEMTNQL
                     LDNMDLERERGITIKAHAIRMVYKRPEGEYILNMIDTPGHVDFSYEVSRSLAACEGAI
                     LVVDASQGIEAQTLSNLYLAIENNLTIIPVLNKIDLPGANPDHIAQLVGDLLGYDPDQ
                     IPRISAKSGINIQAVLDKIVDEIPPPEVNNDGPLQALIFDSVYDSYRGVINYIRIVQG
                     SLSAGQKIKMMKSGAEYMVTEVGTFSMQRDPKPELSAGMVGYVLANVKTISDVKIGDT
                     VTLAQNPSPTALPGYKDILPMIFSGIYPINPEDYKDLREALEKLKLNDSAISWEPETS
                     EALGFGFRAGFLGLLHMEIVQERLDREFNVDIITTVPNVEYHVYMNDGSHIKIESPSK
                     LPDASRYDYIEEPYVKVQIFTPKEFVGAMMTLCDEKRGEFTTMEYIDENKVILKYEIP
                     LAEIMFDFYDRLKSVSRGYAGLDYEPCGYRRNNLVKLDILLNGDPVDAFSVIIHKDKS
                     VQYANAICVKLKELIPRQQFDVAIQGAIGGKIISRSTVKAVRKDVLAKCYGGDITRKR
                     KLLEKQKEGKKRMKNIGSVEVPQKAFLAVLSLSDNSSSAE"
     gene            5814..6344
                     /locus_tag="PLT31_02520"
     CDS             5814..6344
                     /locus_tag="PLT31_02520"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014544935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPW94041.1"
                     /translation="MIFKKTFILLFLFGIVSLAFAEGKLFNKNYEGISQIQAVIITHE
                     GKIVVDLNFKAAPNTVANFVDLAQKGFYKGLVFHRIIQDFMVQGGDPLGNGQGDPGYY
                     IDDEANDLKHEVGVISMANRGPNTAGSQFFITLMPQHHLDGKHTVFGRVIQGQDVVCR
                     IEPNDPIINIEIIEKK"
     gene            6347..7549
                     /locus_tag="PLT31_02525"
     CDS             6347..7549
                     /locus_tag="PLT31_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94042.1"
                     /translation="MSAKKVSPKKAVPVKKAPAKAPAKKAVPVKKAAPAKKTAPAKKD
                     VAVKKATPAKKATSKVAAPKKQVVKAPAKKAVPVKKAAPAKKVVAKKKPVEIKSSAKK
                     TKTPVAAVKKSVPMKKAAPAKKAGVAKSVPSKKKETVKEVLKVSTAKKTPTKKPLKEE
                     EKSSKKIKKVSSNKSAVSPKPSKADTKKLDKKVAEKKSSAKKEKKIVETVVSEEQVEK
                     KEKATKPGKKDNVNNVPEVALAKLLPAGKKPSKVVLFFEIDEQEDRLNKKKVSAEMKG
                     LEKPTAAMRRKASLAEETPEELYQRVIQELEEKNQSFYREAATQVCTKCCVNIVSPEF
                     RVDKDLGYCEDCAMLLGLGFTKEARKVDYQMGLMGGDSLDEDRDEDIDEKAPSIEELE
                     DEDFDIDE"
     gene            complement(7721..8377)
                     /gene="lexA"
                     /locus_tag="PLT31_02530"
     CDS             complement(7721..8377)
                     /gene="lexA"
                     /locus_tag="PLT31_02530"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HPW94043.1"
                     /translation="MDNIDYQSSKNKLTARQQEIFEYIRECTQSSKMPPTVREIGDRF
                     NISSTNGVRSILAALIKKGYIKRSPRLSRGIEILDESSNPAESSSNSIEIPIIGRVAA
                     GQPILAVQNIEGTVTIDRDFLASRTDVFALRVKGDSMINAGILDGDLIFAKQQQSAER
                     GEIIVAQVDNEATIKYFQPQHDWIELHPANPKYHPIIVKPECPFSIAGRVIGVMRKIN
                     "
     gene            8702..9328
                     /locus_tag="PLT31_02535"
     CDS             8702..9328
                     /locus_tag="PLT31_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94044.1"
                     /translation="MEIQIITLILAVIAVVLGVIILSKVNKLTTMLRTPIVKKLSPDM
                     NLKPSSRHAVSAQEMASRGERNNKENRNNHSGKERIANKENNRTERSNTAARNGNDRR
                     DRRHNNRNDRTPRPETTPVQENSSALSSAEQASPVQNQPKPQVQQPETRRPLTPRIQA
                     EVPVAPVVSTPIAAPVAAETAVNDFDPSKVRYGRRNVVKKLPEIADEN"
     gene            9464..10639
                     /locus_tag="PLT31_02540"
     CDS             9464..10639
                     /locus_tag="PLT31_02540"
                     /inference="COORDINATES: protein motif:HMM:NF018271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94045.1"
                     /translation="MIIALLVICFSLYSFLGWVIEVVYRSSIQKTFVNAGFLQGFFVP
                     IYGVVALFFVALDDISNEYPLIIQFILYVVVASALEYIIGFFAEKILHVQLWSYSDNR
                     FNLHGRICLKYSLIWGGLGVFFIEILHPFVWEKVILLNVEQAYIIASILAVYFIIDFA
                     YSISLGRKLGLLLETGYQRIITSDPFELEKWLRQEKRLVQAFSNIRKNMSVAISKKIE
                     NNLGVVLEQYKSILSLKNIDVKFLDDIEYIECVKDILEHPEFLKTKNFKHHQSSIFDH
                     VLKVSFLAYKISRQRGWDYRSAARGGLLHDFFLYDWRDGNDPGRPTKNFHGFRHSKVA
                     LDNSLALFELNKVEQDIIIKHMWPLTPRIPKYKESFLVVFLDKYVATYEFWNKPKKK"
     gene            10701..11402
                     /locus_tag="PLT31_02545"
     CDS             10701..11402
                     /locus_tag="PLT31_02545"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPW94046.1"
                     /translation="MSDYIQNLFAERIGGSSFSIKGSLYKFEKIKQACREIERTHLDT
                     PIIDLGLGKPDWMADSLVIDTFYSEAKKSKITVDDDNVILAFQKAAVQYMDKVYGVSG
                     LDSGQLKAMQYAGIKALEHPEITEKTNMKYSRRHNLLVNALSAVGFTVIKPKAGLYLY
                     TKIPKGVEGGETFKNAEHFAQWLIKEKQISVVPWDDVGHYVRISVTFIANTETEELSL
                     VQEIQKRLSGVRFLW"
     gene            complement(11466..12386)
                     /gene="cysK"
                     /locus_tag="PLT31_02550"
     CDS             complement(11466..12386)
                     /gene="cysK"
                     /locus_tag="PLT31_02550"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HPW94047.1"
                     /translation="MSKVNSIIECIGKTPLIRIQKMNPYENEIYVKMESLNPLSSIKD
                     RVALALIEQAEKDGLLKPNSVIIEPTSGNTGIGLAYVAAVKGYKLILTMPETMSMERR
                     KLLKALGAELVLTEGSLGMRGAIEKADEIASQTKDSFIPRQFDNPANPQIHFETTGPE
                     IWNDSEGNVNIIIATVGTGGTLTGIGSYLKSKKPSVQVVAIEPDTSAVLSGKASGAHP
                     IQGIGAGFIPSVLDTKIIDEIYLTNEEKAFSTARMAVKKEGLLIGISSGAALEAALTI
                     ASRPENKGKTIVAILPDSGERYLSTTLFES"
     gene            complement(12658..13539)
                     /locus_tag="PLT31_02555"
     CDS             complement(12658..13539)
                     /locus_tag="PLT31_02555"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPW94048.1"
                     /translation="MNELIEEDKNIIASANYSTIRDYSLVLASQQIVHDVIPTEEGPF
                     EIWVSKDEAKIARRQIRLFRCENPPHQNNAPLPVMVTLQPLWILSVPALVTLIQFSDR
                     ISHLTSRGISDASMVLKGEWWRLFTALSLHGDAKHVLGNLISGFFVLNLLSLRIPLSR
                     LILPLLLFSGLANGLVSLTMQENFRSLGFSTFVFASLGALSSIEFRLMPKEFHGLQRR
                     FAPLFGALLLAVFMGLGENSDIFAHGYGFILGLVVGFLPKRKSLQWGITFSYLDVIMI
                     ITYFTVFLCCWLLALFY"
     gene            13662..>14032
                     /locus_tag="PLT31_02560"
     CDS             13662..>14032
                     /locus_tag="PLT31_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPW94049.1"
                     /translation="MDTKERFLAEALQLFRREGYESVGIQKIVDAVGVTKPSLYHYFG
                     SKSGLLKELLESHFNPFLRSLESISSYRGDLVYSIEKIIAHYFRFAQENKDFYHWYMG
                     IEHAPEENEVSRFVHPIVVKQ"
BASE COUNT         4406 a         2398 c         2696 g         4532 t
ORIGIN      
        1 atttttaact tgagctaagt gttctgcaat tagaggaatc attccactat gtgctgcttt
       61 acctctaaag ggtactggtg caaggtatgg agcatctgtt tcaagaagca acctttcaag
      121 aggtactatt ttagctgctt ctctcacatt ttcagcattt ttgaaagaga taatccccgt
      181 aaaaccaatg taaatattgg atgataagtt cagaagagct tcagcaaaag caggggttcc
      241 tgtaaagcaa tggacatgga taggatggtt ttgaattgga gcatttctta aaatagaaag
      301 gctatctttt tcggcttctc ggagatgaag tacaagtggc tttctttttt ggaggccaat
      361 ttttagttct ttttcaaata agaggcattg agcttctttg ctttcagcac cgtaatggta
      421 atcaagcccg aattctccac aggcaaccgc tttagggtga tctaaataag attttaatag
      481 ttcctcgtgt tcgggacgat aggaatcaac atattgagga tgtatgccat aggcggcata
      541 aataaaatca tacttttcag acatgatttt agcgtattca aattcgttag gatcacaggc
      601 cacttgaatc atggcttctg gtttgatttc catttttgcc caaagtgttt caaatgattc
      661 ttgagtttgt tcttcgtaag tatctaaatg gcaatgagta tctacaaaag agttcatggg
      721 tatagaacct ctaaaatgac ataggtgagt tcaccgcgtg gtgtttgtac tgatattttt
      781 tctccggttt ttttccctaa aagagctgtt ccaagaggag atttcatgct gattttacct
      841 tgaagagggt caatttcttc acccccaacg atggaataag ccttcaattt ctgagttttt
      901 tcttccttta attggatttt tgctccaaaa cggatcgaac catcgacagg agcttttgct
      961 tcgacaatct gggctccatc taaaagctta tctaattgac gtaaacgtcg atcgatttca
     1021 cgtaaacgca tacgaccaaa agtataagcg gcattttcag aacgatcgcc ttcagcagcg
     1081 gcgtcgctga cctgtttttg cattgctggt cgttcgatat atttaagaga ttcccattcg
     1141 gctttgaatt tttcaaaacc ttctttagaa atatgatgct gcatataaca ataatagaaa
     1201 aaagaattat ttattactaa atttgaattc atgaaaaatc caattgtaaa aagaaaacat
     1261 cctgactcac gttccactcg tattggacga gtcatgttta atagaatgtt tccaaagcgt
     1321 ttagatgcat taaaagtggc tttctctgta gggattggtg tttttatagg cattgctcca
     1381 acaattggaa ttgctgttat tttaactgtc gctgtatgtg cagctttaaa attacctaaa
     1441 gtgcccgccg ttgtcgcttc ctttatagcc aatccattta ctcagtttgg attcttttat
     1501 cctgtaggtt atatgattgg aaaatctatc gtagagccta aaaaaatatc gtttgatttt
     1561 ttaggcgaat tagagcgttt atcttttaga aattttacag atattatttc tcatttatgg
     1621 atcaatgctg cagatcattt gctttctttt atgattggca ttatgctatt agcttttgta
     1681 tttggtcttg ccttttttgg ggctgcttat tttattgttt cttatagaaa aagaaagcat
     1741 gttgaagtta aaaacgccta tattcgaaac ttagttatag aagatgaaca attaattaaa
     1801 gaagcaaaac aacaaggaaa atcatttcat atgcatatct atccatttaa agcgcttcgt
     1861 ccagttcgtt cggaagatgc aaaagatata tcagccctcc cttatgatgt tatgaataga
     1921 gcagaggccg ctaagatggc ggaaaacctc cctcattcgt atttgcgagt gactcgtgcg
     1981 gaacttgagc ttccagaaat atctgatgct tatgatccga aggtttatct tcgtgcgaaa
     2041 gaaaatttag ataaaatgat agccgatggc gttattgccc atgaaaagaa aaattgcttg
     2101 tatgtttatc gtcaagtgat ggaagggcga gaacagtatg gtctagtttg ttgtgtacct
     2161 gcaaaagatt attttcataa aattattaaa aagcatgagt tgactcgagc tgataaagaa
     2221 gaagatcgtt tacgtcatgt gcttgctact aatgctaata cagggcctgt ctttttaact
     2281 tatcgagatc aaggtcaatt cgatcttttt ggagctgttc gttcccgtaa acctatttat
     2341 gactttgtga ctgaagcaga tggctttgga catacggttt ggttaattga agacgaacaa
     2401 gaaattcaaa ccatttgtga atcttttaat catgtgcctg tttcttatat tgctgatggt
     2461 catcatcgta gtgctgcagg ggctcgtgct tcaggttatc gcgctgaaca aaataagaat
     2521 aatacaggtg aagaagagta taatcgtttc ttagctattt tattcccttc cacacaatta
     2581 aaaattatgg actataatcg tgttttgaaa acatggaatg gccatacacc agaatccatt
     2641 ctaaaagcac tagaaccaat ttttaatgtt aagaaattga atgacatgat tcatcctcaa
     2701 caaatgcatc aagttcatat gtatttgcag ggttcttggt atcaatgtga gtttaaggat
     2761 gagttgttaa aggatttagg tccagtagat agcctggacg tctctctatt acaaaagcat
     2821 gtattgaatc ctttctttgg aatcgatgac ccaagaacat caaaagatat tgattttgtt
     2881 gggggtattc gtgggctttc tgagctagtg aaaagagtgg atagcggtga atgttcttgt
     2941 gcttttgcta tgtatccaac tactttagat caattgatgg caatcgctga tgctaatgaa
     3001 atcatgcctc caaaaagcac ttggtttgag ccaaaactga gagatggtct attagttcac
     3061 accttagatt aattccaagg aagtctaatt ttcttcccga tgattgttga tggagagaat
     3121 aaccccaatt ctctcgaatc aatgcatcgg gcgccttgat tacaagcaag gtagtagtag
     3181 tcttctaaaa caataaaaga ttctatttta gacgagtctt ttaaagaaaa aacttctctt
     3241 tttattttta cttcgcctac actaggctga cttttttgta cttgtaatcc tattaaaaaa
     3301 agctcttgcc aaggaagccc ttgaatttct ttaacgctta caggtcttga tgcgattctt
     3361 gcactcccta ccacatttaa aggtaattct tgagatcctt gaaaaacgct tggtttaata
     3421 aagaaagaag tgttttgctg ttttagaatg gaaatggcta gatccatttc aacatcattt
     3481 aagctatcca aaaaaagagt gtctcctttt tttggaatat aaaagtgtct tgaatctata
     3541 aatgcagttt catttttcca aacaaaatca agataagcag atttgacatg accttcagaa
     3601 gaaattttaa gttctgaacc aggaagccca attacacgac gtaataaaac ttctccaatt
     3661 ctcttttggg caagaacaat atcattggga tgaatttgat ttttacacca aggagtctta
     3721 caaatccata gacgctcatt gtttttgatt tcaggagcca tggagttgtc tttaatttgt
     3781 atgtgttcaa tggcgtaaaa acgggtgaga atagatagag taaaaacaac tattaaaaca
     3841 agaaaaataa aaaatacgcg agtttgcgaa tttttaacaa ttaatttttt taatagtttt
     3901 tctgatgggt tcattttatt ctgcacttga agaattatcc gaaagagaaa gaacagcaag
     3961 gaaagccttt tgaggaactt ccacagaacc tatgtttttc attctcttct tgccttcttt
     4021 ttgtttctct aagagtttcc gtttacgagt aatatcacca ccatagcatt tggcgagaac
     4081 gtctttacgt actgctttca cagtacttct gctgatgatc tttccaccaa tagccccttg
     4141 aatagctaca tcaaactgtt gtcttggaat taattctttc aattttacgc aaatggcatt
     4201 ggcgtattga actgatttat ctttatgaat aataacagaa aatgcatcta cgggatcacc
     4261 atttaataaa atatctagct tgaccaagtt attgcgacgg taaccacaag gttcatagtc
     4321 taaaccagca taaccgcggc tgaccgattt taatcgatca taaaaatcaa acatgatttc
     4381 tgctaatgga atttcgtact taagaatgac tttattttcg tcgatgtatt ccatggtcgt
     4441 aaattcacca cgtttctcat cacaaagcgt catcatggcg cccacaaatt ctttaggtgt
     4501 aaaaatctgt acttttacat agggttcttc gatatagtca tagcgactcg catcaggaag
     4561 ttttgaagga ctttcaattt taatatgaga accatcattc atataaacgt gatactccac
     4621 gtttggaacc gtggtgatga tgtcgacatt aaattctcga tctaaacgtt cttgcacaat
     4681 ttccatgtgc agaagcccaa gaaagcctgc tctaaagcca aaaccaagag cttcagatgt
     4741 ttctggttcc catgaaatgg cagagtcatt taattttaat ttttctagag cttctcgtaa
     4801 atctttatag tcttcaggat taattggata aattccagag aaaatcattg gaagaatatc
     4861 tttataacca ggtagagctg taggtgatgg attttgagca agtgttacgg tatcgccaat
     4921 tttcacatca ctaatggttt tgacgtttgc aagaacatag cccaccattc ctgcactgag
     4981 ttcaggttta gggtctcttt gcatggagaa tgtgcctact tctgtcacca tatactctgc
     5041 gccacttttc atcattttga ttttttgacc agcagataga gatccttgaa caatgcgtat
     5101 atagttgatg actccacgat agctatcgta aacagaatca aaaattaaag cttgaagagg
     5161 cccatcattg ttgacttcgg gaggaggtat ttcatcaacg attttatcta agaccgcttg
     5221 aatattaatt cctgatttag ctgatattct ggggatttgg tctggatcat aacctaatag
     5281 atctccgact aattgtgcga tatgatctgg atttgctcca ggtaaatcaa ttttattaag
     5341 aacaggaata atcgttaaat tattttcaat cgcaagatat aagttggata gtgtttgagc
     5401 ctctatccct tgactagcgt cgactacgag aatagctcct tcacatgcgg ctaaggaacg
     5461 actgacttca taagaaaagt ctacgtgccc tggtgtatca atcatattta gaatatactc
     5521 gccttcaggc cttttgtata ccattctaat ggcatgagcc ttaattgtaa tgcctcgttc
     5581 tcgttctaaa tccatgttat ctaataattg atttgtcatc tcattagacg ctacggtgtg
     5641 agtcatttca atcattctat cggcaagagt tgattttccg tggtcaatgt gggcgataat
     5701 actaaaattt ctgatgaggt tatgcttttt tgacatattt ctacatttta taaaggttca
     5761 agacaaatat agttatgtat atttgttcca gtattttaaa tatgggtatt ctgatgatat
     5821 ttaaaaaaac ttttattctt ttattccttt ttggtatagt ctctttggct tttgctgaag
     5881 gtaaactatt taataaaaat tatgaaggta tttctcaaat tcaagcggtt attatcacac
     5941 atgagggtaa aattgtagtg gatttgaatt ttaaggccgc tccaaatacg gttgcgaatt
     6001 tcgtggatct ggctcaaaaa ggattttata aagggttagt ttttcatcgt attattcaag
     6061 acttcatggt tcagggcggt gatcctttag gtaatggtca gggtgatcct ggttattata
     6121 tcgatgacga agctaatgat ctgaagcacg aggtaggcgt tatatctatg gcgaatcgtg
     6181 gccctaatac cgctggatca cagtttttta taacactaat gcctcagcat catttagacg
     6241 gaaaacacac tgttttcgga cgagtgattc aagggcagga tgtcgtttgt cgtattgaac
     6301 caaacgatcc tattatcaac attgaaatta ttgagaaaaa ataagtatga gcgcaaagaa
     6361 agtgtctcct aagaaagcag ttccagttaa aaaggctcct gcaaaagcac ctgctaagaa
     6421 agctgttcca gtcaagaagg ctgctcccgc aaaaaaaacg gctcctgcaa agaaggacgt
     6481 tgctgttaag aaagcgactc ctgcaaaaaa ggctacatca aaagtagcag ccccaaaaaa
     6541 acaagttgtc aaagcacctg caaagaaggc tgttccagtc aagaaggctg cacctgcaaa
     6601 aaaagtggtt gcaaagaaaa agccagtaga aataaaatct tctgcaaaaa aaacaaagac
     6661 tcctgttgct gctgtaaaaa aaagcgttcc aatgaagaag gctgctcccg caaaaaaagc
     6721 gggtgtggca aagagtgtcc cttctaagaa gaaagaaacg gttaaggaag ttttaaaagt
     6781 ttctactgct aagaaaacgc ctacaaagaa accacttaaa gaagaagaaa aaagttctaa
     6841 aaaaataaag aaggtttctt caaataagag tgctgtatct cctaaacctt ctaaagcaga
     6901 cacaaaaaaa cttgacaaaa aagtagcaga aaaaaagagt tctgcaaaaa aagaaaagaa
     6961 aatagttgaa acagttgttt ccgaagaaca agtagaaaaa aaagaaaaag caactaagcc
     7021 tggaaaaaaa gacaatgtta ataatgttcc tgaagtggca ttagctaagc ttttgcctgc
     7081 agggaaaaag ccttcaaaag tcgttctttt cttcgaaata gatgagcaag aagatcgctt
     7141 gaataaaaag aaggtttctg cagagatgaa ggggcttgaa aagcctactg ctgctatgcg
     7201 tcgaaaggct tcacttgcag aagaaacacc tgaagaactt tatcaacgag tgattcaaga
     7261 gctagaagaa aagaatcaat ctttttatcg ggaagcggct acgcaagttt gtacaaaatg
     7321 ttgcgtgaat atagtctctc ctgaatttcg tgtagataaa gatttaggct attgtgaaga
     7381 ctgtgctatg cttttagggc ttggttttac taaggaagct cgtaaggtcg attatcaaat
     7441 gggcttgatg ggtggtgatt ctttagatga agatcgagac gaagatattg atgaaaaagc
     7501 tccatctata gaagaactcg aagatgaaga ttttgatata gatgaataat aagttttttc
     7561 ttcagaaagt tacataaaaa aagtcgaact cttgagttcg actttttctt tttaaataaa
     7621 tactgtttta ttgtgatatt tcaaatctta gagattattc cattcgcaat acgatgattt
     7681 aagcaagaac gagtaaaatg gctaatcgga ttctatcttc ttagtttatt tttctcataa
     7741 cgccaatcac tcgaccagca atggaaaaag gacattcagg tttaactata ataggatgat
     7801 attttggatt tgcaggatga agttctatcc agtcgtgctg aggttgaaaa tatttaatcg
     7861 tagcttcatt atcgacttga gcaacgatga tttctcctct ttcggcagat tgttgctgtt
     7921 ttgcaaaaat taaatcaccg tctaaaatgc ctgcattaat catggagtcg cctttgactc
     7981 gaagagcaaa aacatctgta cgacttgcta gaaaatcccg atcaatggtg acagttcctt
     8041 ctatgttttg aacagcaaga ataggttgcc cagcggcaac acgaccgata attggaattt
     8101 ctatggaatt actagaggat tctgcaggat ttgaagattc gtcaagaatt tcaatccctc
     8161 ggctgagtct tggggaacgt ttgatgtagc ctttttttat taaagcggct aaaatagagc
     8221 gaactccatt agtagaggaa atgttaaaac gatctccaat ttctcgaact gtaggaggca
     8281 tttttgaaga ttgtgtacat tcacggatgt attcaaaaat ctcttgttga cgagctgtaa
     8341 gcttattttt ggatgattga taatcgatat tgtccatttt aacctctttg atctaattaa
     8401 atatactgca ttattgtgca tttgtcaata taggcactgc acattaattc aaaaaaatgc
     8461 tttttcttct attttatctt ttttttgatt tgaaaaaccc tttaaggtct tctttttatg
     8521 taaatttgcc gcgtggtttc agctttttcg cttaagcgaa aaatgagcta tagaaagctc
     8581 cttgccgtca agtctttaag gcgttagtaa gatggcactc attttaattt ttgtaaacca
     8641 ccctgagaac aggttatttt ccagcttgtt ttcgaggcta actctatata tgaggtgact
     8701 aatggaaata caaatcatta cgctaattct tgctgttatt gcagtagttt taggcgttat
     8761 tattctttct aaagtgaata agcttacgac aatgcttcgt acgccgattg taaaaaaact
     8821 ttctcccgat atgaacttaa aaccatcttc tcgtcatgct gtaagcgcac aagaaatggc
     8881 ttctcgtgga gaacgtaaca ataaagaaaa tcgcaataat cactctggta aagaacgtat
     8941 agctaataaa gaaaataatc gtactgaacg tagtaatact gctgctcgta atggaaatga
     9001 tcgcagagat cgtagacata acaatcgcaa tgatcgtaca ccacgtccag aaacgacccc
     9061 agttcaagaa aatagttctg ccctttcttc ggcagagcag gcttctccag ttcaaaatca
     9121 accaaaacca caggttcaac aaccagagac acgtcgtccg ttaactcctc gcattcaagc
     9181 agaagtaccg gtcgctccag ttgttagcac tcctattgca gccccagtgg cagctgagac
     9241 tgcggtgaat gattttgatc cttcgaaggt tcgctatggc cgtcgtaatg tagtcaaaaa
     9301 actccctgag attgctgacg aaaattaata cggtttttga agaattaaaa aaaaggctct
     9361 ctttctagag ggcctttttt gttttacagg caatcataca aggattaagg atatatagaa
     9421 gaaactgata ttttagaata gtgctttttt tatattatga aatatgatta ttgctttatt
     9481 agtaatttgt ttttccttat attctttttt agggtgggtg attgaagtcg tttatcgttc
     9541 ttcgattcaa aagacttttg tgaatgcagg ctttcttcaa ggtttttttg ttccaatata
     9601 tggtgttgtt gctttatttt ttgtggcttt agatgatatt tcaaatgagt atcctttaat
     9661 cattcagttt attctttatg tcgtcgtggc gagtgcttta gaatatatta taggcttttt
     9721 tgctgaaaaa atacttcatg tacagttatg gagctattct gataatcgtt tcaatttgca
     9781 tggtcgaata tgcttaaagt attctttgat ttggggtggc ttaggtgttt tttttataga
     9841 aatacttcac ccatttgtat gggaaaaagt aattttattg aacgtagaac aggcttatat
     9901 aatcgctagc attttagctg tctattttat tattgatttt gcttattcta tttctcttgg
     9961 tcgaaagttg ggcttacttt tagaaacagg gtatcagcga atcattactt ctgatccttt
    10021 tgaattagaa aaatggttgc gtcaggaaaa acgattggtc caagcttttt ctaatataag
    10081 gaaaaatatg tctgtagcga tttcaaaaaa aatcgagaat aacttaggag ttgttttgga
    10141 acaatataaa tcgattttat cattgaaaaa tatagacgta aaatttctag atgatattga
    10201 atatatcgag tgtgttaagg atattttaga acatcccgag tttttaaaaa ctaaaaattt
    10261 caaacatcat caaagtagta tttttgatca tgttcttaag gtttcttttt tggcttataa
    10321 gatatcacgt caaagagggt gggattaccg ctcggctgca cgtggtgggc ttcttcatga
    10381 tttcttctta tatgattggc gtgatggcaa tgaccctgga cgacctacaa aaaattttca
    10441 tggctttcgt cattcaaaag tggctttaga taattcatta gcattgtttg agttaaataa
    10501 ggtagaacaa gatattatta tcaagcatat gtggccttta actccacgta ttcctaaata
    10561 taaagaatct tttttagttg tctttttaga caagtatgtt gctacttacg aattttggaa
    10621 taagcccaaa aagaaataaa aactcttgaa actaagagtc atattttatc atttcaaatg
    10681 ataagtttaa tggagtgtga atgtcagatt atattcaaaa tctttttgct gagcgcatag
    10741 gtggttcgtc ttttagtata aaagggagcc tctataaatt tgaaaaaata aaacaagctt
    10801 gtcgtgaaat agaaagaact catctcgata cgcctattat tgatttgggt cttggaaaac
    10861 ctgattggat ggccgattcc ttagtcatag atacttttta tagtgaagca aagaaatcga
    10921 aaattacggt ggatgacgat aatgtcattt tagcttttca aaaagctgcc gttcagtaca
    10981 tggataaagt gtatggtgtt tctggtctag attctggtca gcttaaagca atgcaatatg
    11041 caggtataaa agctttagag catcctgaaa ttaccgaaaa aaccaatatg aagtattctc
    11101 gtcgtcataa tttattagtg aacgcgctct ctgcagtagg ttttactgtt ataaaaccaa
    11161 aggcgggtct ttatctttat acaaagatac ccaaaggtgt agaaggcgga gaaacgttta
    11221 aaaatgcgga acatttcgct caatggctga tcaaagaaaa acaaatttca gtagtccctt
    11281 gggatgatgt gggccattat gtgcgtatta gtgttacctt tatagcaaat acagaaacag
    11341 aggaattgtc tttagtgcaa gaaatccaaa agcgtttgtc tggagtacgt tttctctggt
    11401 aaggtcttga gtaaaggttt tgtttgatta aaagaagagt gaattatatt aataattcaa
    11461 aaaagctacg attcaaaaag agtagtggat aaataacgtt ctcccgaatc aggaagaatc
    11521 gctacaatcg ttttcccttt attttcaggc cttgaagcta ttgtgagagc cgcttctaat
    11581 gcagctcccg aagaaatacc tatcaagagg ccttcttttt ttactgccat tctagcggtt
    11641 gaaaatgctt tttcttcatt agtgagataa atttcgtcaa taattttagt gtctaaaaca
    11701 gaagggataa atccagctcc aatcccttgt attggatgag ctccagatgc ttttccagag
    11761 agaacagctg aggtatcggg ctctatggcg accacttgaa cggaaggctt tttagatttt
    11821 aaataagaac cgatacctgt taatgttcct ccagtaccta ctgtagcaat aataatattc
    11881 acattccctt cagaatcatt ccatatttct ggtcctgtgg tttcaaaatg aatctgtgga
    11941 tttgctggat tatcaaattg gcgaggaata aaagagtctt tcgtttgaga tgcgatttca
    12001 tcggcttttt cgatagcgcc acgcattcct aaactccctt ctgtcaggac gagttcagca
    12061 cctaaggctt tgagaagctt tctacgttcc atgctcatcg tctcaggcat agtgagaatt
    12121 agcttatacc ctttcacggc cgctacataa gcaagtccaa tccctgtatt tccacttgtg
    12181 ggctcaataa ttactgagtt aggctttaat aaaccatctt tttcagcttg ctcaattaaa
    12241 gcaagagcca ctcgatcctt aatactcgaa agaggattta gcgattccat tttcacataa
    12301 atttcatttt catacggatt cattttttga attcgtatta atggagtttt gccgatgcat
    12361 tcgataatac tatttacttt agacattttt actccgtaaa catggaaaaa ttgtgtatga
    12421 aaaataaaaa aagttggttt tataagtata aaaaggaact tttcttgaaa tacaatattt
    12481 tacaaacata aaatatattt tcatccagtg atttctatat ccattttttt aggctgcttt
    12541 ttagctaagt ccttcggagg atctcgcttg agttacacga aggaagtaat aatagactcg
    12601 tattgcaagc tgttctgcgt gggtagaaaa agaaagataa agcattgaaa tctatttcta
    12661 ataaaataga gccaaaagcc agcagcaaag aaatacagta aagtaagtaa ttatcataat
    12721 gacgtccaaa taagaaaatg taatacccca ttgtagcgat ttcctttttg gtaaaaagcc
    12781 tacgactaag cctaatataa aaccatagcc gtgagcgaaa atatcagaat tctcaccaag
    12841 ccccataaag acagcaagaa gtagggctcc aaataatgga gcaaaacgtc tctgcaaacc
    12901 atgaaattct ttgggcatta aacgaaactc aatcgagcta agtgctccaa gagaagcaaa
    12961 aacaaaagtg gaaaaaccaa gagaacgaaa attttcttgc attgttagac tgacgagacc
    13021 attcgctaaa ccagagaata gtaatagtgg aagaatcagt ctagataatg gaattcgaag
    13081 agataataag ttcaaaacaa aaaagcctga gattaaattc cctagaacat gtttagcgtc
    13141 gccatgaaga gaaagggctg taaacaaacg ccaccattct cctttcaaaa ccatagacgc
    13201 atccgatatg cctcgagacg taaggtgaga aatacgatca gaaaattgaa ttagagtgac
    13261 aagtgctgga acagataaaa tccatagagg ctgtaaggta accattactg gaagaggcgc
    13321 attattttga tgaggaggat tttcgcatcg aaagagtcga atttgtcttc gtgcaatttt
    13381 tgcctcatcc tttgaaaccc aaatttcaaa aggcccttct tctgttggaa tcacatcgtg
    13441 aacgatttgt tgagaagcta aaacgagact ataatctcta atagtggaat aattagcgga
    13501 agcgatgatg tttttatctt cttcaatgag ttcattcatg aaaacaaaat accaatctta
    13561 tagataactg gctgaagaga ttctttattt taaggctttt tgctgttatt cttttttttc
    13621 tcctcttgac gaatagtata ccaattggta tattatagtc tatggataca aaagagcgat
    13681 ttttagcaga agctttgcag ctttttcgtc gtgaaggtta cgaatctgta gggattcaaa
    13741 aaatagtgga tgccgttggt gtcactaagc cttcgttgta tcattatttt gggagtaaat
    13801 caggcctact aaaagaactg ctcgagtctc attttaatcc ttttttacgc tctttagaat
    13861 ccatttcctc ttatcgtggc gatttggtct attctataga aaaaatcatc gctcattact
    13921 tccgctttgc acaggaaaat aaggattttt atcattggta tatgggcata gagcatgctc
    13981 cagaagaaaa tgaagtttca cgttttgttc atccaattgt ggttaaacaa tg
//
LOCUS       DAOOBB010000030        13960 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_5651_length_13960_cov_5.044588, whole genome shotgun sequence.
ACCESSION   DAOOBB010000030 DAOOBB010000000
VERSION     DAOOBB010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 13960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13960
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5651_length_13960_cov_5.044588"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..422
                     /locus_tag="PLT31_02565"
     CDS             84..422
                     /locus_tag="PLT31_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94050.1"
                     /translation="MDNDLLFDRFDETNFSVSIQDGHIVINYEYMRGEAVNEYAYENG
                     NWILVYASSSHRTCCHASISSYDYRTKTLTESTYRVGDEDYDESTGIPRDTVITTIEE
                     RPVIYMDQPF"
     gene            complement(439..1779)
                     /gene="glmM"
                     /locus_tag="PLT31_02570"
     CDS             complement(439..1779)
                     /gene="glmM"
                     /locus_tag="PLT31_02570"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HPW94051.1"
                     /translation="MSKLMRSVSGIRGIVGDSLTPTTLLSHVKAFLEITKAKKIVIGR
                     DSRPTGEAISTFVTAFCRLAGTEVIDAGLSTTPTVEILVTETKADGGIIITASHNPVE
                     WNALKFLDRKGLFLGPSDAESLFKIADQGDFKFPDYTQVATSAILKDADDIHINGTLG
                     IEFVDTQAIKNKRFKVALDAVNGAGSFILPRLLKALNCEVVPIHCSPNGKFPRGAEPL
                     PENLSDLSKAVLEHHCDVGFALDPDADRCALVDGNGNAIGEEYTLAIAMEEVLFQKKG
                     SVCVNLSTSRMNEDVAQKYGCVCTRAKVGEINVSLQMMQEKCIIGGEGNGGVILPALH
                     YGRDSLVAAALVLSWMAHHHGGPEKFVSENPSYFMPKKKFNLSNKPLSSILEEVKIEF
                     KDWEMDSRDGLRFAKNKSWVHVRASNTEPVLRVIAEAPSENEAYDLCKRVEQHI"
     gene            complement(2003..2869)
                     /locus_tag="PLT31_02575"
     CDS             complement(2003..2869)
                     /locus_tag="PLT31_02575"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HPW94052.1"
                     /translation="MKYAYIEIANTCNLNCSFCPYSQKEHFPSWMSVDLFSKIIDDIK
                     NNVEEIYLHVLGEPLLHPDLKTFLEIADQAKLRVNITTNGTLIKKNHLLLLESTVVRQ
                     INFSVHALEEWNNPEKALSALNDMLLFAQEAAEKRPDLYINFRLWNIQKENSSHWNRL
                     VSSQLESTFHCDLSTHDFSIRHKSFPLQNRIYLHFDTRFEWPDGKQEGLSTKGTCKAL
                     DSHIGILCDGRVVACCLDHNGNLELGKLPQQTLSSILSSPRAIAMKQGFKEKKLVEPF
                     CQSCGFCKRFKV"
     gene            complement(2866..3696)
                     /locus_tag="PLT31_02580"
     CDS             complement(2866..3696)
                     /locus_tag="PLT31_02580"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HPW94053.1"
                     /translation="MDFLWVLFALIYRFFYKMNRFVFLRPQKPLQKSKLIIIGSYLMG
                     GAGKTPFTLYLAKSFLKENFTVAILCHSKAMDEYEWLQQEALDAKVFSTKNRFRLAKK
                     IDGLFDIIIADDGFEDFRLSAHHKIALQWGEFAHDIIDLFPSGYCRSLKKDHPDVTMT
                     LYCGNSFIDPDLVFSISEIKNEQGDSLNKSATAMCGIGNPDRFFSDLQDFGITIEKKI
                     IRPDHDRKFELMLKKELLLGKPIVITEKDCCRLTLETRKIPLIFVAKQKITVLKEFHL
                     "
     gene            3879..4673
                     /gene="rsmA"
                     /locus_tag="PLT31_02585"
     CDS             3879..4673
                     /gene="rsmA"
                     /locus_tag="PLT31_02585"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HPW94054.1"
                     /translation="MDRARRRRFGQNFLDDSTAKLIADDLPVNNQAILEIGPGHGAMT
                     KHLLKKNVPLTAVEIDDQCATLLKDRFAEDSNFSIVNVDFLKFDLLQWLEEHPLSWIT
                     GNLPYNVSTAIIAGLMPRLSKTSGFMGMVQLEVAERLCAVPCTKAYGSLSVWVRSYGE
                     PRLLRKIGPEHFTPKPNVDSATIMIVPRENPIKAPEGFFDWVQLAFSMKRKRIANSLS
                     TSYEKELVFKALSILNLSENTRAEELSPEQFLDLYQLLLKLKSQTN"
     gene            complement(4817..6649)
                     /gene="yidC"
                     /locus_tag="PLT31_02590"
     CDS             complement(4817..6649)
                     /gene="yidC"
                     /locus_tag="PLT31_02590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HPW94055.1"
                     /translation="MNRNTIIGSVLIAAIMIWWMTTMPKPQPAPQKVEKAIASEVSQD
                     VKNESKNSLEIPSLKSNTDSTSSKAPAFLSSNDTLAKDSLASDSLIELPPPVLEKSIV
                     VETDLFILKLSNRGAKVSSVIIKTLTDNEGNFPELIEDTSAGALDLKLDKTNLSDALF
                     AVSPETPSKIVVNQDTTIQFVFSDPNRNKVIRSYTFSKTGSAVRQSNRFEGFQPNDYE
                     ILWNGGMRETELFPKGKNIGANYYFSEIIYNNTYSVEREVIQEKKKFNDEDGKIIWAG
                     LRRKYVAMSIQFDEPTDAVVQGEPLKGSKEKNNPGTYQLSVSDYLKGADSLSFNFMIL
                     PLEWTIIKSLDQDYEKVIVSGWQWIGADVWFVALCGLLLSLLKIFYSFIPNYGIAIIL
                     LTILVRIATTPLTIKQLRSTKEMGKIKPELDAINVKYRAEPQKKQAAIMELYAKHNIN
                     PMASCTGGCFPMLLQFPIFIGLFIILARAIELRGMPFIGWISDLSRSDVIWNGITIPY
                     IMPEGLSILPLIMVVTTYFQTKQSMSAMTDPAQRKMMIWMMPGMMFVFSAVMPSGLVL
                     YWIISNLWGIGQYAIIHREKAVASPTNAKIQDAEIIKPKKKKKK"
     gene            complement(6646..6900)
                     /gene="yidD"
                     /locus_tag="PLT31_02595"
     CDS             complement(6646..6900)
                     /gene="yidD"
                     /locus_tag="PLT31_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HPW94056.1"
                     /translation="MILKSIKKICQLILILPIRAYQYIHPSFFRGTCRFYPTCSHYAI
                     EAIETHGALKGFYLAVFRIIRCNPFCKGGYDPVPPRKESL"
     gene            complement(6897..7217)
                     /gene="rnpA"
                     /locus_tag="PLT31_02600"
     CDS             complement(6897..7217)
                     /gene="rnpA"
                     /locus_tag="PLT31_02600"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HPW94057.1"
                     /translation="MRSPFQFRQISAQGKFVRSPSLTMRWIKSPDSVCRFCFLVKKKN
                     GNAVYRNRCRRILRPIFFESSSQMKEPVWAMIFINENFKEMTPEKLRESANKLLSKME
                     KSLL"
     gene            complement(7251..7406)
                     /gene="rpmH"
                     /locus_tag="PLT31_02605"
     CDS             complement(7251..7406)
                     /gene="rpmH"
                     /locus_tag="PLT31_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HPW94058.1"
                     /translation="MKRTFQPHNRKRSNKQGFRARMEDRWGRAVISRRRAKGRKRLTV
                     SDTIYKK"
     gene            complement(7919..8227)
                     /locus_tag="PLT31_02610"
     CDS             complement(7919..8227)
                     /locus_tag="PLT31_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPW94059.1"
                     /translation="MNKQDLIGAVLADKAAGIETKAAAERAVNAVIAAITTGIKKDGN
                     VQLIGFGTFQVKERSARMGRNPKTGAAMKIKASKTVGFKAGAALKETAKKTKIAKKIF
                     "
     gene            complement(8331..9482)
                     /locus_tag="PLT31_02615"
     CDS             complement(8331..9482)
                     /locus_tag="PLT31_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPW94060.1"
                     /translation="MHKHWTADRIMQFIFALIIITIFILLLNYLSAVLVPFGAAFLIA
                     YILDPIVNKLQVKVKFRIISVLIVLFSFAALLTIALIIFIPQVTEEIQRLGSLLSKLL
                     TDSSWRGRLNEVIPENLWDGLRSLISMESIAESMRSLDFWKDVQSILSKVVPGALGVL
                     SGTATVIFWIVGAALIIMYLIFIMLDMPKLRANIKKLIPLKYKEEAAELAKEMDYFMT
                     SYFRAQTMVAFTVGILFATAFSIIGLPMGFLFGLFIGALNMVPYLQIVSIPLALLLGI
                     VYGLDTGIPFWEVALLITAIYATIQVIQDMLIVPNIVGRSMNLPPVGILLSLSVWGKL
                     LGFLGLIVAIPFTCLCLVYLDKIKRNQDAQDLFWESKEEETPPKPSVTP"
     gene            complement(9475..10614)
                     /locus_tag="PLT31_02620"
     CDS             complement(9475..10614)
                     /locus_tag="PLT31_02620"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPW94061.1"
                     /translation="MSKNIKLSILYVLCIIALVTIWSLFHSKQLEIFPSNSFPKSVIY
                     SYGDESDGGFSTASFTKNDSSLILSLLVRSGIAYPYAGMGIDLISDTQKLSGQFIDFS
                     SFDSLSITLRTKYMKQVQVKLITHDPNLTRLEDPMSARYLLQVIPSDRSYQNIHIPIS
                     SFSIPEWWFERNGFKAPDQLKYLERGIKIDISSGSGTMLGIADEIEIKKITLFGSNHF
                     LNKMILSLAIIITALFLGFLWIDHSKRLTEQKIEVKLRKEQALENYSKLPLESHQKQE
                     AERVIDYINSNYQNASLSLEDVCREVGLNRNKLSEILKKNVGMTFSSYVNEIRLKEAC
                     RLLIETDLQVIEISINIGFGNVSHFNKVFKQTYQKTPLEYRRAHA"
     gene            complement(10611..11327)
                     /locus_tag="PLT31_02625"
     CDS             complement(10611..11327)
                     /locus_tag="PLT31_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94062.1"
                     /translation="MKKLYLILGGLSAIALIIYALIPNREFRESLYPISESTIVSTYD
                     DQSDGGTSSAGLSIVDSTLLFKCKLHNNQDKPAWCGLIWDLDPEGKKNYQNWTFVDSL
                     ILNISSQNINEIIIKVWTFDPDVTNPEKKNSFRPLIKEWVLKGGVERIAIPMEALYIP
                     TFWYEQNNVDKKLKQKHQETVARFEITPGWNVSRDLEFSISIQSIEVKGLSNLAFGVI
                     LFFFLVIVSIAIGFRQKNKK"
     gene            complement(11324..11848)
                     /locus_tag="PLT31_02630"
     CDS             complement(11324..11848)
                     /locus_tag="PLT31_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94063.1"
                     /translation="MTKNCPFCQTPVSSIKLHHFTLRICPHCYSTFFPANNTMSFRNE
                     ISDKTREIWLSVLKSKNIKDPVVEGDVKCIDHGEPLIKGLLPDYAIEGYVTTCCDMFH
                     LPPSLTCQILERTLKSPFHATRRSKYAKNHFFFIHIFDKILSKIIGEKHTEDDPLDLI
                     QYELHFKDILGPKP"
     gene            12109..12933
                     /locus_tag="PLT31_02635"
     CDS             12109..12933
                     /locus_tag="PLT31_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW94064.1"
                     /translation="MSQILEITLENFDSMIMAASKERPVVLHFFSSRYPECATFGALL
                     EKEASLLDFTLGTVNLDVPENNQFIQVFRVQSLPDVRIISGGQIIDAISGIMPEDKLR
                     ARLEKHFLSDEKRYLMNVESLIEQKFWDEALPQIESVLKEKPGNKPYLLLKAKALLGQ
                     GQVALAKEILESFIASDDEYTSARSYLSLIDFHVEVAKTEIHGEEAQIYHEACLLAVQ
                     SEYKKSLQRFLDLVQLNKDWNNGAASKAMLTLFGVLGPKHELTWVFRSKLNTILFI"
     gene            13051..>13960
                     /locus_tag="PLT31_02640"
     CDS             13051..>13960
                     /locus_tag="PLT31_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin attachment protein"
                     /protein_id="HPW94065.1"
                     /translation="MKKILFQDTSFRDGFQSIFGARAFTKDFTPAVEAACAAGIQHFE
                     AGGGARFQALYQNVGEDAFDMMDEFRRIVGPNVRLQTLARGINVVALEAQPKDMIKLH
                     ADMFKKHGMTRIRNFDALNDVNNLIFSGQCIHDAGLEHEVVITMMELPLGCTGAHDPE
                     FYIKTLKDILNAGIPYSSICFKDASGTANPRKVYETIKLARKLLGDSVEIRMHTHDTC
                     GTGVAQYVAAIEGGCSGVDLARKPLSGGTGQPDLFSLYHALKGTDYKLALGDGIVDDQ
                     IPKIMEANNVTVECLKDYQFPPEALQI"
BASE COUNT         4438 a         2399 c         2708 g         4415 t
ORIGIN      
        1 cgagggataa ataataaaat tacatttgac taaatgcagt tcgtcggacg ggtacgtttt
       61 atgcgataga ttcttttgaa gatatggata atgatttatt gttcgatcgt tttgatgaaa
      121 ccaatttttc tgtttcaatt caagatggtc atattgtaat taattatgaa tatatgagag
      181 gggaggcggt gaatgaatat gcttatgaaa atgggaattg gatattagtg tatgcttcat
      241 caagtcatcg aacgtgttgc catgcatcca taagttccta tgattataga acgaaaacat
      301 taacagaatc tacttatcgt gttggtgacg aagattatga tgaatccaca ggaatcccgc
      361 gcgatactgt tattacaact atagaagaaa ggcctgttat atatatggat caaccttttt
      421 aataaaggct atcttaaact agatatgttg ttctactctt ttgcataaat cataggcctc
      481 attttctgat ggggcttctg caatgacgcg aagaacgggc tctgtattag aagcgcgcac
      541 gtgaacccag gatttgtttt ttgcaaaacg aagaccatct cttgagtcca tttcccaatc
      601 tttaaattca attttcactt cttctaaaat gctactcaat ggcttgttgc ttaaattgaa
      661 tttcttttta ggcatgaaat agctagggtt ttcgctcaca aacttttctg ggccgccatg
      721 atgatgagcc atccaactga gaactagagc ggcagcgaca agagagtctc ggccataatg
      781 aagagcaggt agaattactc cgccgtttcc ttcaccacca ataatgcatt tttcttgcat
      841 catttgaaga ctaacattga tctcgcccac tttagctcga gtacaaacgc atccatactt
      901 ttgagcgaca tcttcgttca ttcgagaggt agaaaggttt acacatacag agcctttctt
      961 ttgaaaaagc acttcttcca tggcaatagc aagggtgtat tcttctccaa tagcattgcc
     1021 attaccatcg actaaagcac agcgatcggc atcagggtcg agagcaaaac caacgtcaca
     1081 gtgatgctct aaaacagctt tagagagatc acttaaattt tcaggaagag gttctgcacc
     1141 tctagggaat tttccattag ggctgcaatg gattggaacg acttcacaat ttagggcttt
     1201 tagtaagcga ggtagaataa aagaacctgc tccatttacg gcatctagtg ccactttgaa
     1261 acgcttgttt tttatcgctt gagtgtctac aaattcaatc cctaaagtgc cattaatatg
     1321 aatgtcatcg gcatctttga gaatggcgct agttgctact tgagtgtaat cagggaattt
     1381 aaaatcacct tgatcggcta ttttaaataa cgattctgca tcgcttgggc ccaaaaataa
     1441 acctttgcgg tctaaaaatt ttagggcgtt ccattctact ggattatggc ttgctgtaat
     1501 aatgatgcca ccatccgctt tcgtttctgt aactaaaatt tcaactgtcg gggtggtact
     1561 taggcctgca tcaatcactt cagttcctgc taaacgacaa aatgcagtga caaaagttga
     1621 aatggcttct cccgtgggtc tagagtctcg gccaatcaca attttttttg ctttggtgat
     1681 ttctaaaaaa gcttttacgt gagaaagaag tgtggtcggg gtgagagaat cgcctacgat
     1741 gccacgaata cctgatacag agcgcattaa ttttgacatg aaaatacctg cattataaaa
     1801 aaggacacct ctaaaaattc atttacggcc aaaatagttt tgaatcttcg caatagttcg
     1861 ttgttcaaag tagcaaataa caccaattgt cgccatttta agcacctgct tttgcttgat
     1921 caatttgatt ttgtctttcg ttaaccaatt tttagaaaat cactaaagta aaaaataaaa
     1981 catttttaga agcttgttaa aattaaacct taaaacgctt acaaaatcca cagctttggc
     2041 aaaaaggttc taccagtttc ttttctttga acccctgttt catggcaata gctcttgggc
     2101 tagataaaat agagcttagt gtttgttgag gaagttttcc taattcgaga ttcccattat
     2161 gatctaaaca acaagcgaca acacgtccat cacataaaat gcctatatga gaatcgagtg
     2221 ctttgcaggt gcctttggta gagagccctt cttgtttgcc atcgggccat tcaaagcgag
     2281 tgtcaaaatg aaggtaaatt cgattttgaa gcggaaagct tttgtggcga atagaaaaat
     2341 catgagtgga aagatcacaa tgaaaagtgg attccaattg gctagaaacg agtctattcc
     2401 aatgagaaga attctctttt tggatattcc aaagtctaaa attaatatag agatcggggc
     2461 gtttttcagc agcttcttgt gcaaagagaa gcatatcatt taacgcagac aacgcttttt
     2521 ctggattgtt ccattcttct aaagcatgta cagaaaaatt gatttgccta acaactgtag
     2581 attctaataa tagcagatga ttctttttaa tgagagttcc attggtggtg atattcacac
     2641 gtagctttgc ttggtctgca atttctaaaa aagtttttaa gtctgggtgt aaaaggggtt
     2701 ctcctaaaac atgtaagtat atttcttcga cattgttttt gatatcatca atgattttag
     2761 aaaacagatc tacactcatc catgaaggga aatgttcttt ttgagaataa gggcaaaagc
     2821 tacaatttaa attacaggtg tttgcgattt caatgtaggc gtatttcata gatggaactc
     2881 ttttaataca gttatttttt gtttggcgac aaaaataagc gggatttttc ttgtttctag
     2941 agttagacgg cagcaatctt tttctgtaat gacaataggt ttgccaagta agagttcttt
     3001 tttaagcatc agctcaaatt tacgatcatg atctggacgg atgattttct tttctatggt
     3061 gattccaaaa tcttgaagat cagaaaagaa gcgatctgga tttccaatgc cacacatggc
     3121 cgtggcactc ttatttaaag aatctccctg ttcatttttt atttcagaaa tagagaaaac
     3181 aagatcgggg tcaataaaag aatttccaca ataaagagtc atcgttacat cagggtgatc
     3241 ctttttaagg ctacgacaat aacctgatgg gaataaatca attatatcat gagcaaattc
     3301 gccccattgg agagcgattt tatgatgggc agagagtcta aaatcttcaa aaccatcatc
     3361 agcaataata atatcgaata gtccatctat ttttttggct aagcgaaagc gattctttgt
     3421 tgaaaagact tttgcatcta aagcttcttg ttgtaaccac tcatactcat ccattgcttt
     3481 agaatgacat aaaatggcga cggtgaaatt ttcttttaag aaagatttag caagatacag
     3541 agtgaaaggc gttttccccg cgcctcccat aagataagaa cctataataa ttagtttgga
     3601 tttctgaaga ggcttttgag gacgcaaaaa aacgaatcga ttcattttat aaaagaaccg
     3661 atatattaaa gcgaataaaa cccaaagaaa atccatcgaa aaaaaataga atataaataa
     3721 gtcaagtaaa agactatttt ctggatcttc tgataaaaaa aggaggatat ttaatgtgat
     3781 taatcctatt taattaagcg agtgttaatt tgtttcattt aagtctttat ttttttcaca
     3841 attctattaa tgagtaagga ataactaaat ttgattttat ggatagagca cgccgtcgac
     3901 gatttggaca aaacttttta gatgattcaa ctgcaaaatt gattgcagac gatttacctg
     3961 tgaataacca ggctatatta gaaattggcc cagggcacgg tgccatgaca aagcatctat
     4021 taaaaaagaa tgtcccattg acggcagtcg aaattgatga tcagtgtgct acattactca
     4081 aagatcgttt tgcggaggat tcgaatttct ctatagtcaa tgttgacttt ttgaaatttg
     4141 atcttttgca gtggttagaa gagcaccctt tgtcttggat aacagggaac ttaccttaca
     4201 atgtttctac agctatcatt gcgggcttaa tgcctcgttt gtcaaagacc tcgggtttta
     4261 tgggcatggt gcaattggag gtcgcagaac gcttgtgtgc cgttccttgc actaaagcat
     4321 atggaagcct ttcggtatgg gttcgctctt atggagaacc acgcctatta agaaaaattg
     4381 gccctgaaca ttttacccca aagccaaacg tggatagcgc aaccattatg attgtgcctc
     4441 gagaaaatcc gataaaagca ccagaaggtt tttttgattg ggttcagctt gcattttcta
     4501 tgaagcgaaa gcgcattgct aattctttaa gtacctctta tgaaaaagag cttgttttta
     4561 aagccctttc tattctcaac ctatctgaaa acactcgtgc cgaagagcta tctccagagc
     4621 agtttttaga cttatatcaa ttgcttttga aattaaagtc acaaacgaat taaatcacaa
     4681 gaaattgttc ttgtaagcaa ctttaataaa tacaagaagt atttattgag actgaatatt
     4741 attgacttct gtagttgaaa aaagggtaaa caaaaaacga cgattttact cgccgttttt
     4801 tgtttgagag aatacattat ttctttttct tctttttagg cttgattatc tcagcgtctt
     4861 gaattttagc atttgtaggc gaagcaacag ccttttcacg atgaataata gcatattgtc
     4921 caattcccca taagttggag ataatccaat ataacacaag accagaaggc atcactgcac
     4981 taaagacaaa catcatacca ggcatcatcc aaatcatcat tttacgttga gctggatcgg
     5041 tcatcgcaga catagattgt tttgtttgga aataagtagt gacaaccata attaagggta
     5101 gaatagaaag accttctggc ataatataag gaatggtgat tccattccag attacatcgg
     5161 atctagacaa gtcactaatc caaccaatga atggcatacc tctaagttca atggcacgag
     5221 caagaataat aaagagacca ataaaaatag ggaactggag tagcattggg aagcagccac
     5281 ctgtacaaga ggccattggg ttgatattat gcttagcata aagctccata atagcggctt
     5341 gttttttctg aggttctgca cgatatttta cattaatggc atcaagttct ggcttgattt
     5401 tacccatttc ttttgtggag cgcaattgct ttatggttag tggtgttgtt gcaatacgaa
     5461 ctaatattgt taataagata atggcaatac cataatttgg gataaatgag tagaatattt
     5521 ttaataacga taataataat ccacaaagag caacaaacca aacatcagcg ccaatccact
     5581 gccatccact gacgattact ttttcataat cttgatcaag agatttaatt attgtccact
     5641 ctagtggaag aatcataaag ttaaagctaa gggagtcagc gccttttaga taatcgctta
     5701 cgcttaactg ataagtacct ggattattct tttctttcga tccttttaat ggttcccctt
     5761 gaacaaccgc atcggtgggt tcgtcaaatt ggatgctcat cgcgacatac ttacgacgaa
     5821 gccctgccca aataatcttt ccatcttcgt cattaaattt ctttttttct tgaatgactt
     5881 cgcgttctac actataagtg ttgttataaa tgatttcgct gaagtagtaa ttagcaccga
     5941 tgttcttgcc tttaggaaac aattctgttt cgcgcatacc gccattccag agaatttcat
     6001 aatcatttgg ttgaaatcct tcaaagcggt tagattgtct aacagcagag cctgtttttg
     6061 aaaaagtata tgaacgaatt actttattcc ggttaggatc ggaaaaaaca aactgaattg
     6121 tggtgtcctg gttcacgact atttttgatg gcgtttctgg tgaaacagca aataaagcat
     6181 cacttagatt tgttttgtct aatttaagat caagcgcacc agcagaggtg tcttctatga
     6241 gttctgggaa atttccttcg ttatcagtaa gcgttttgat aatgacgctt gaaactttgg
     6301 caccgcgatt agaaagtttt aagataaata agtctgtttc tacaacaata cttttttcta
     6361 aaacaggggg tggcagttca attagagaat cacttgcaag agagtctttt gctaaagtgt
     6421 cattagagga caaaaaagca ggtgctttac ttgaagtaga atcagtattg cttttgagag
     6481 atggaatttc aagagaattt tttgattcat ttttgacatc ttgagacact tcagaagcaa
     6541 tagccttttc cactttttga ggtgctggtt gtggctttgg cattgttgtc atccaccata
     6601 tcataatggc agcgataaga acagagccta taattgtatt tctattcata atgattcctt
     6661 acgtggagga acgggatcgt agccaccctt acaaaaaggg ttgcatcgaa taatccgaaa
     6721 aacagcaaga tagaaacctt ttaaagctcc gtgagtttca atggcttcaa ttgcatagtg
     6781 agagcatgtt ggataaaagc gacatgtccc tctaaaaaaa gaggggtgta tatactgata
     6841 agcgcgaata ggaagaatta agatcaattg gcaaattttt tttattgatt ttaagatcat
     6901 aaaaggcttt tctccatttt agacaaaagc ttatttgccg actcacgtag tttctctgga
     6961 gtcatctctt tgaagttttc gttaatgaaa atcatagccc agacaggctc tttcatttga
     7021 gaggaggatt caaagaaaat agggcgtaaa atacgtctac aacggttacg ataaacggca
     7081 ttaccattct tttttttgac taaaaaacaa aaacgacaaa cactgtcagg actttttatc
     7141 catctcatcg ttaatgacgg tgagcgaaca aatttacctt gggcagagat ttgtcgaaac
     7201 tgaaatggcg atcgcagccg ataagatctt aaagaggcaa taacccgacc ttatttttta
     7261 taaatagtgt cactaacggt taagcgtttg cggcctttgg cacgacgacg actaataaca
     7321 gcacgtcccc aacggtcttc catacgggca cgaaaacctt gcttattgga acgtttacga
     7381 ttgtggggtt ggaatgttct tttcatgatt ttaaaccttt aaaaattgca attcagaagc
     7441 ccaaatctaa taaaacgctt atattaatca aggggttatc ctgtacttag gggtttaaaa
     7501 gagaaaataa gggcacaaat aagcatttta ttgaagggtt tcttttaatt agagattaga
     7561 aaataggatc ttcaggagga acgcaaatgg ggttttgtat aagtcataca gaaaaacgaa
     7621 aaataataaa gccttagaaa gaagaataat tatgtatttg ttttctaaaa tatttcattt
     7681 cgttttgaca attgggatac aggtacggta aaaaaagact tcatgcgaca aaaagagagt
     7741 taattaaaat gatttatatg aagtattcgc gaggtgtatt tttttttcat gataatgaaa
     7801 aagtctttga aataaaaagt tgaaaaaatg ggcgctaaaa aagattagga tttgtgggtt
     7861 gcttttatgt gataggggta taaaaaaaga accgctaata aagcggctct ttttttgttc
     7921 agaaaatttt cttagcaatc tttgttttct tagctgtttc ttttaaagca gctccagcct
     7981 tgaagccaac ggtcttagaa gctttgatct tcatagcggc gccagtttta ggattgcgac
     8041 ccatgcgagc actgcgttct ttaacttgga atgtaccaaa accgatcaat tgaacgtttc
     8101 cgtctttctt gataccagta gtgatagctg caataacagc gttaactgcg cgttctgcag
     8161 cagcttttgt ttcgatacca gcagctttat ctgcaagaac agcaccgatg agatcttgtt
     8221 tattcattat gactccttga tagttgttga aaattcaatg taagatatta tacctttttt
     8281 ttggccttaa gcaagttttt ttttcattta tttctgttta agcccctatt ttagggggtt
     8341 acagacggtt tgggaggggt ttcttcctct tttgactccc aaaataagtc ctgggcgtct
     8401 tggttacgct ttattttatc gaggtaaact aagcataaac aagtaaatgg aatagcgaca
     8461 ataagcccta agaagccaag taatttaccc caaacagata aagaaagaag gattcctaca
     8521 ggtggtaaat tcatacttct tccaacaata tttggaacta taagcatgtc ctgtatgact
     8581 tgaatcgtag cataaattgc tgttataagt aaagcgactt cccagaaagg aatacctgta
     8641 tcaagaccat agacaatccc taataacaat gcaagaggga tacttactat ttgcagataa
     8701 ggaaccatgt ttaaggctcc aataaatagg ccaaataaaa agcccatagg aagtccaatt
     8761 atggagaagg ctgtggcaaa aagaatacct acagtaaagg caaccatggt ttgtgctcta
     8821 aagtaggagg tcataaaata atccatttct ttagcaagtt ctgctgcttc ttctttgtat
     8881 ttcaaaggga ttaatttttt gatattggct cttaactttg gcatatcaag cataatgaaa
     8941 atcaaataca tgataatcaa agcagcacct acaatccaaa atataacagt agccgtacca
     9001 gataaaacac ctagagctcc tggaacgact ttagacaaaa tggattgtac atccttccag
     9061 aaatcaaggc tacgcatgga ttcagcaata ctttccattg aaattaaact acgtaaacca
     9121 tcccaaaggt tttcaggaat gacttcgttt agacggcctc tccaggaaga gtctgttaaa
     9181 agctttgata atagagagcc gagtctttgg atttcttctg tgacttgagg aataaaaatg
     9241 attaaagcaa tggtgagtaa ggcggcgaat gaaaacaaga cgataagaac ggatatgata
     9301 cggaacttga cttttacttg taatttatta acgataggat ctaaaatata agcgattaaa
     9361 aaagcggcgc caaaaggcac aagaacagcg cttaagtaat tcaataataa aatgaatata
     9421 gtgattataa tcagagcaaa aataaattgc atgatccgat cggcggtcca atgtttatgc
     9481 atgggctctc ctatattcta aaggggtttt ttgataagtc tgtttgaaaa ctttattaaa
     9541 atgactcaca ttgccaaagc caatattaat tgaaatttca atcacctgaa gatcggtttc
     9601 aattagaaga cgacaggctt cttttaatcg aatttcattg acgtaactac taaaagtcat
     9661 acctacattt tttttcagaa tttcagaaag tttgtttcga tttaagccca cttcgcgaca
     9721 aacatcttct agagaaagag aagcgttttg atagttcgaa ttaatataat cgatgactcg
     9781 ctcagcttct tgcttttgat gactttctaa agggagcttg ctgtaatttt ctaatgcctg
     9841 ttctttacgt agctttactt cgattttttg ttctgttaat ctttttgaat gatcaatcca
     9901 tagaaaacca agaaataaag ccgtaatgat gattgcaaga ctaagaatca ttttatttag
     9961 aaaatgattg cttccaaaaa gggtgatttt tttaatttca atttcatcag caatgccaag
    10021 cattgttcct gatccacttg aaatatcaat tttaattcct cgttcgagat attttagctg
    10081 gtctggggcc ttaaaaccat tacgttcaaa ccaccattct ggaatagaaa aactggaaat
    10141 aggaatatgt atgttttgat aagaacgatc agatggaatc acttggagta aatatcttgc
    10201 agacataggg tcttcaagac gagttaaatt aggatcatga gtaatcagtt tgacttgaac
    10261 ttgcttcatg tatttggttc taagagtaat acttaaagag tcaaaagaag aaaagtcaat
    10321 aaattgtcca gaaagttttt gagtatcaga gattaaatca attcccatgc ctgcataagg
    10381 gtatgcaatt ccagatcgaa cgagtagcga tagaattaag gaagaatcat tttttgtaaa
    10441 agaggctgta gagaatcctc catcggattc atcgccataa gaataaataa cagattttgg
    10501 aaatgaatta gaaggaaata tttctagctg tttggaatga aataaagacc aaatcgtaac
    10561 aagagcgatt atacagagaa cataaagaat agaaagttta atatttttac tcatttttta
    10621 tttttttgac gaaatccgat ggcgattgaa acaatgacaa gaaagaaaaa taaaataacc
    10681 ccaaaagcca gattactaag ccctttgact tcaatcgatt gaatggaaat agaaaattct
    10741 aagtcacgag agacgttcca tccaggagtg atttcaaaac gagccaccgt ttcttggtgc
    10801 ttttgtttta gctttttatc tacattattt tgttcatacc aaaaggtagg aatataaagc
    10861 gcctccattg gaatcgctat tctttcaact ccacctttga gaacccattc tttaatgaga
    10921 ggtcggaaag aattcttttt ttctggattt gtgacatcag gatcaaaggt ccaaacttta
    10981 ataatgattt catttatgtt ttgactagaa atgtttaaga ttaaagagtc aacaaaagtc
    11041 cagttttggt agttcttttt cccttcgggg tcaagatccc aaattaatcc acaccaagcg
    11101 ggtttgtctt gattattatg aagtttgcat ttaaataaaa gagtagagtc tacaatagaa
    11161 agccctgctg aagaggttcc accatcggat tgatcatcat aggtggatac gatagtactt
    11221 tctgaaattg gataaagtga ttccctaaac tctcggtttg gaatgagagc atatattata
    11281 agtgcaatag cagacaaacc gcctaaaatt aggtatagtt ttttcatggt ttagggccta
    11341 aaatatcctt aaagtgaagc tcgtattgaa tcaagtcgag ggggtcatct tcggtgtgct
    11401 tttcaccaat aatttttgaa agaattttat cgaaaatatg aataaagaag aaatgatttt
    11461 ttgcgtactt acttcttctt gtggcgtgaa aaggtgattt taaggtacgt tcaagaattt
    11521 ggcaggtcag tgatggaggt aaatgaaaca tgtcacaaca tgtggtgaca tagccttcaa
    11581 tcgcgtaatc aggaagcaat cccttgatga ggggttctcc atggtctatg catttcacgt
    11641 caccttcaac aacgggatct ttgatgtttt ttgatttaag aacagaaagc cagatttcac
    11701 gtgttttatc agaaatttca tttcgaaagc tcatggtgtt attagctgga aaaaacgtcg
    11761 aataacaatg aggacagatt cgcaaagtga agtggtgtag tttgatactt gaaacagggg
    11821 tttggcaaaa agggcaattt tttgtcatag cgttaccatt aaatattgat ttttaaaatg
    11881 agaatagaat agagtcttca ttttgatgcc ataatcgcat aacaaagcca tccaatggca
    11941 atgaattcca ataaaaagaa tgaagagtga ttttttagcg gcaaacatat aagattaata
    12001 taataaaagc aaaataggct ttttttcact tttttaagaa attaggcaaa ttaggaacat
    12061 attgttaaaa tgagacgttt atcttttttg tttttttaaa ttgattctat gtcacaaatc
    12121 ctagaaatta cacttgaaaa ttttgattcc atgattatgg ccgcttctaa ggaaaggcct
    12181 gtggttttgc atttcttttc ttccagatac ccagaatgcg ctacatttgg ggctttgctt
    12241 gaaaaagagg cttcactttt agactttact ttaggaacag ttaatttaga tgttcctgaa
    12301 aataaccagt ttattcaagt ctttcgagta cagtctcttc ctgatgttcg gatcatttct
    12361 ggtggccaga ttatagatgc catttcagga attatgccag aagacaaatt gagagctcgt
    12421 ttagaaaagc actttctttc agatgaaaaa cgctatttga tgaatgtgga aagtcttatt
    12481 gaacagaaat tctgggatga agctcttcct caaatcgaat ctgttcttaa agaaaagcca
    12541 ggcaataaac cttatttact tttgaaagca aaagcgcttc ttgggcaagg tcaagtggct
    12601 ttagcgaaag agattttaga aagttttatc gcctctgatg atgagtatac ttcagcacgt
    12661 tcgtatttgt ctttaataga ttttcatgtg gaagtcgcta aaactgagat tcatggagag
    12721 gaagctcaaa tttaccacga agcttgtctt ttggctgttc aaagtgaata taaaaaatct
    12781 ttacaacgct ttttagattt ggttcaactg aataaagatt ggaataatgg cgcagcgtct
    12841 aaggcaatgc ttactctttt tggcgtttta ggcccaaaac atgaattaac gtgggtgttc
    12901 cgttctaaat tgaacacgat tttatttatt taatgatacc tctaaaaatc gagattttag
    12961 gggtgtcctt attctcattt ttttttgtat ctttagatca gtattgatag accaatagac
    13021 cggggtgacg aaccccacag gaaaaacaga atgaaaaaga ttctcttcca agatacctcc
    13081 ttccgtgatg gattccaatc cattttcgga gctcgtgcat tcacaaaaga ttttacgcct
    13141 gctgtagaag ccgcttgtgc tgcaggaatt cagcattttg aagctggtgg tggtgctcgt
    13201 tttcaggctc tatatcaaaa cgtaggtgaa gatgcttttg atatgatgga tgaattccgt
    13261 cgcatagtcg gcccaaacgt tcgtttacaa acattagctc gcggtattaa cgttgtggct
    13321 cttgaagcac aaccaaaaga tatgattaag cttcatgccg atatgtttaa gaaacatggt
    13381 atgactcgta ttcgtaactt tgatgcctta aacgatgtaa ataacttgat tttctcagga
    13441 caatgcattc atgatgcagg ccttgaacat gaagtcgtga tcaccatgat ggaactccca
    13501 ttaggttgca caggagctca tgaccctgaa ttttatatca aaacgctaaa agatattttg
    13561 aacgctggta ttccttattc ttctatttgc tttaaagatg cttctggtac agctaatcca
    13621 cgcaaagtat atgaaaccat taagcttgct cgtaagcttt tgggcgattc tgtagaaatt
    13681 cgtatgcata cgcatgatac atgcggcact ggagttgctc aatatgttgc tgctattgaa
    13741 ggtggctgta gtggtgttga cttggctcgt aagcctcttt ctggtggtac aggtcaacct
    13801 gatttattct ctctttatca tgcacttaag ggcacggatt ataagcttgc tttaggtgat
    13861 ggtattgttg atgatcaaat tcctaagatc atggaagcaa ataacgtgac tgtggaatgt
    13921 ttgaaagatt atcaattccc accagaagct ctccagattt
//
LOCUS       DAOOBB010000031        13895 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_5677_length_13895_cov_3.677095, whole genome shotgun sequence.
ACCESSION   DAOOBB010000031 DAOOBB010000000
VERSION     DAOOBB010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 13895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13895
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5677_length_13895_cov_3.677095"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="PLT31_02645"
     CDS             <1..491
                     /locus_tag="PLT31_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94066.1"
                     /translation="WITGDGLDSISKAFQWVNIRQDMTNLNWIMSAGFYLGNWKFYFE
                     RGEVIHRNVKLLDTHSLYYKGSIFWSNRFVKERLGVSVRFDFQWFGDRYDCAVNKDGV
                     PEIIPLNKYLALNFEAKMQILSFELYSRIDNLNHSLYKPAAGYTPEGVRFSYGIIWSF
                     SN"
     gene            complement(497..1228)
                     /locus_tag="PLT31_02650"
     CDS             complement(497..1228)
                     /locus_tag="PLT31_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016603 - glutaminyl-peptide
                     cyclotransferase activity [Evidence IEA];
                     GO_process: GO:0017186 - peptidyl-pyroglutamic acid
                     biosynthetic process, using glutaminyl-peptide
                     cyclotransferase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HPW94067.1"
                     /translation="MSIYLSLFFAAALQSFSIVDSLPHSTSHFTQGLNFNGKEWIEST
                     GLHGKSFLYRKTLSGQVLDSISLDERYFGEGSVQVGDVIFWLTWKNRKGFIYDAKPFQ
                     LRGSFRIPSEGWGLSFWNGTLLMSNGSHELYRLSPGDFRVVQVINVLDGNKPIKNLNE
                     LEVVGNTLYANIWQSDSIAIIDLPSGKVRAYLDLSELSQRIRKSHRNVDVLNGIAVDG
                     DFLWITGKNWPKIYKLKSPSFKHQK"
     gene            1264..1836
                     /locus_tag="PLT31_02655"
     CDS             1264..1836
                     /locus_tag="PLT31_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HPW94068.1"
                     /translation="MGAIKKPLLVKLIIGILCGDPKFMDHVRPILSQKFGKEEEILPL
                     FPFVFTNYYEEEIGDKPLRAFLSYERLISREEIVDIKLWTNDLELEIAKEQGTGEKRP
                     INLDPGYMTLGQFFLATTKDQRQRVYIRDGIFVEPTLYFQAGHFHPFDWTYRDYQSET
                     YIEYLEKVRQKLAYQHATGKPYRERSTPLK"
     gene            1846..2202
                     /locus_tag="PLT31_02660"
     CDS             1846..2202
                     /locus_tag="PLT31_02660"
                     /inference="COORDINATES: protein motif:HMM:NF016245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HPW94069.1"
                     /translation="MLKTLLNPHVLTVLMLICSNCFMTFAWYGHLKNMSHKAWYIAAF
                     ASWGIALFEYLIMVPANRFGYQVYTLPQLKIIQEVVTLSVFIPFAVFYMKQDFKLDYI
                     WACLCLLGAVYFMFRN"
     gene            complement(2549..3484)
                     /gene="mdh"
                     /locus_tag="PLT31_02665"
     CDS             complement(2549..3484)
                     /gene="mdh"
                     /locus_tag="PLT31_02665"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HPW94070.1"
                     /translation="MARKKIALVGAGQIGGTMALALAQKQLGDVVLVDVVPGVPQGKA
                     LDIMEGRSVINSSTQLSGSNDYADLAGSDVVIVTAGVPRKPGMSRDDLLGINYGIIEK
                     VGLALKATAPNAFVIVITNPLDAMVYAMQKVTGFPAKQVVGMAGVLDSSRLACFVAME
                     LGVAAEDVSAVVMGGHGDTMVSLYNYVSVGGIPLPQLMDKETFDKLAVRTANAGGEIV
                     ELLKTGSAFYSPGLSAIKMAEAYLLDKKSVITCAAKLNGEYGVNDLYCGVPVVIGAGG
                     VEKILEVAMDASEKAAFDKSVAAVKNNADWVNAKG"
     gene            3584..4873
                     /gene="hisS"
                     /locus_tag="PLT31_02670"
     CDS             3584..4873
                     /gene="hisS"
                     /locus_tag="PLT31_02670"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPW94071.1"
                     /translation="MTTTKVQPPQLPKGTRDFYPEDMRIQNFIFDTWKRISLRFGYEE
                     YEGPMFEHLELFTGKSGDEIVSQLYNFEDKGGRAISLRPEMTPTLARLVIQKGNAIKK
                     PFKWFSIPRLFRYERAQKGRLREFFQLNLDVIGTESIFAEADLISAIASMLLEFGLKP
                     TDFLIGISSRKLLSTYLEEIGTPSAELVYPVLDRRLKIGPEAFAEALTAIGLSEDQTK
                     KIDSLMCCKSIEDVRGLVSSPASIEALKEIEDLFDILTSTGFGECIGLDLSIVRGLAY
                     YTGIVFEVFDKGKSMRAIAGGGRYDSLCEKLGGAKVPGVGFGMGDVVLADLLRDHDLL
                     PTQKQTLDLFIASFTLDMKKIFKTANLFRQMGFNVSHPLSPIKLGKQMELANQQGASC
                     VVYVDGENAKEGEFEFKDLIKGTMNVGSAQKISEYLR"
     gene            4873..>6368
                     /locus_tag="PLT31_02675"
     CDS             4873..>6368
                     /locus_tag="PLT31_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94072.1"
                     /translation="MSPFKTLCSIIILFVALGLLSVVFPEKGISFFNVTLRFPTLSDM
                     FPKAEDKPTEKAEDPEIAIQRMLEETRQREFSVYADSLHFYESFFKKGATRFDLPNND
                     PTWFDRFFLHLELASLEEKVVHIIHYGDSQIEEDRISGTIREELQEVFGGSGPGMLPP
                     ILEVPSMTTSHWNSGDLERFILFGPKEQEATHNRYGPLAQFANLNGSASIQIKKRPNR
                     KKQFPHVGEYSQIRVLTGKEKSLNISLSYDSTYIENEGDPEKEKKKKKAVNAPAPKVE
                     RFEKLNVYQWILPEPTDVARLSLSGEAEIYGIAVDGRFGVALDNVPMRGSSGTIFHRI
                     DKTLLKESYDALNARLIIMEYGGNLVPGTNRGNVEWTKKIITRQIHTIQSVNPNADIL
                     FIGPADMAKQINGQLQTYPGLPLVIKALREVCEENGVAYWDMHRVMGGNGSMQKWVKR
                     TPALGHTDYIHFTRQGAAHIGNLFSSSLKTYYDFFKFRDAHKIDDNKL"
     assembly_gap    6369..6468
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6602..8062
                     /locus_tag="PLT31_02680"
     CDS             6602..8062
                     /locus_tag="PLT31_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HPW94073.1"
                     /translation="MNRWIGLLLLFSSLLAANENTLDFANYTFIDTSINEIHFPAGDE
                     SYKHFYEKMDSLVFENKGQVRIVHFGGSHIQADVFSNRVRENLVKRYPGLAAGRGFVF
                     PYSAARTNTPSSYASYYKGKWDMSKNVLKEITKPLGLLGIAVSTTDPRAEISIFLNQY
                     NNTPIWFFNEVRVFGFSELNDVVPIVKLDSTVYQGIPDTVNQSFVFNLPRSTDSLIIS
                     FRWTDSLKQDSLIQVFKQDSILQDSLSKDSLIKDSISKGVSKTDVVSADSMFQDSLII
                     SDTIPDLHHKFTLTGILVENQHPGISYTNVGINGAKVQAYLVCPNLERDLSFFKPDLI
                     IFSIGINDANVEHFNDKKFQEEYDSLITLIQKVAPKAAILFTTNNDSYRKVRRNRYIQ
                     HPNGKVAREAFLNMAQKHKAGVWDLFTIMGGLGSMAKWEKADLAKKDKVHFKSSGYKL
                     IGDLFYEALLKSYNNHVAHLPAEKPSLEKIEKGTKP"
     gene            8059..9909
                     /locus_tag="PLT31_02685"
     CDS             8059..9909
                     /locus_tag="PLT31_02685"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HPW94074.1"
                     /translation="MIDRLISYFSLIFSFDQNAPLLFTQFYFWAFFAIVFAVFSLIHN
                     KFALRNAFLFFVSLFFYYKTSGSYVLILIFTVVINFYLAKWIHRNTSISWRRFGVILA
                     VIVNLLTLSYFKYTYFFLDLIQQVFGIELHAYNFFNAASNYLFKTESLVDRIVLPVGI
                     SFFTFQAISYIVDVYRKTVTPVKKLLDFGFYLTFFPQLVAGPIVRANVFIPQLYKQSY
                     LPRRTFGLAVFWILNGLAKKIIMSDYLAVNFVDRVFETPLLFTGFENLLALFAYSLQV
                     YADFSGYTDIAIGVALLMGFHLPKNFNSPYKAINPTGFWRRWHMSLSTWLRDYLYIPL
                     GGNRRAGFGTFFWIAIISIIATILSGSLIVGLVLVLFFIGLGIYAYFHAEDRRSITTQ
                     MNNMTTMLLGGLWHGASWNFMIWGGLNGAGMILNRLWCRFQTPVRAILMFAMTTTFII
                     LSKHFFHPLFAILAVWGGLIFVGLYFNLIFRLFTDKTINWVYQSWNILLTFIFITFTR
                     LFFRSGSNLDPVEANETAWETATNMIRQIGSPWNLDTVPTMIFEYKNIILVFALGMII
                     HWLPDRFKRLYRYVFANFALPIQIILTALSVFLIYQFMSADSQPFIYFQF"
     gene            9951..11819
                     /locus_tag="PLT31_02690"
     CDS             9951..11819
                     /locus_tag="PLT31_02690"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPW94075.1"
                     /translation="MAARLTQLVQLYFTSIPSGGLLTEQPIKSFICASIVEISTFAII
                     AFLFSIVFLIKQKKWHSFFYAFFASIGVIYLLFSAINDQVMRWMGQHFDLAFIKTYSV
                     SNLEPKLFLNIASNGIVSFIISSLIVLITAWLLYFIPKKITFKNHSYFSLGLSSIVLF
                     ALFIISFTAIDWYKPVRIRWKQIQPIVATWYYDIKYDLTHKNKTEDILIGIEELGGDI
                     NSDYPFYHFVANEDSSLQAFKSKPLEERPDIILLSLESLRGWASDFRISENCSRMPNL
                     CALSNSGTSFPYTYSVGFPSTEGMTGLQLGIWSHPQKVFLTDFISSNSRALPEILGDA
                     GYYRLFLTAAEPSFDNFTPWFNKWADYHEYKKENDSDIPLAHRFVELYKKRPKDKPLY
                     SIWINFTTHTPFTVPKSYASPANTSEERYKQVLTYLDSALGIIFKAIQEDDRHHETIL
                     VITGDHSIANNASQKASSQNGNAFSGQTWTTMTWYGYRVPKEKKILSPISHVDFAPTI
                     LSLLDLSVSNHFVGTPLFDEENVHHTVYSFRRGGIARRQDSIICFTDVDPSVPSLYKK
                     HNSFIDWDTTNTIEGFASEPDLESPHDFNNLEHRARAAAESWRYILDHNKLFPTQK"
     gene            complement(11886..11958)
                     /locus_tag="PLT31_02695"
     tRNA            complement(11886..11958)
                     /locus_tag="PLT31_02695"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11923..11925),aa:Lys,seq:ctt)
     gene            12032..12934
                     /locus_tag="PLT31_02700"
     CDS             12032..12934
                     /locus_tag="PLT31_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HPW94076.1"
                     /translation="MTNGSLLDLYCSHLSIERNLSSATVRSYQEDLRHFIFWLDDHDI
                     SLRDLDLPLLDQFLNQLALNVEYSPTPIARHFSSLRGFLKFMQQEGYYSHSTESMLAV
                     PKIGLYLPQCLNLDEVDSVFKHITEFSKYPLRDTALFELLYSAGLRISEALNLKLEHL
                     DLENEWLTPVGKGNKQRLVPLGSKAKMNLQKWIKEGRPETEPKSDCVILNLKGRPMSR
                     MGAWKIVQQHTAHLTKHVSPHTFRHSFATHCLEAGMDLRVLQELLGHADIATTQIYTH
                     IDKEFIQREHHLHHPREIKATPND"
     gene            12985..>13895
                     /gene="leuB"
                     /locus_tag="PLT31_02705"
     CDS             12985..>13895
                     /gene="leuB"
                     /locus_tag="PLT31_02705"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HPW94077.1"
                     /translation="MSKNYKIAVLPGDGIGPEVMKEAVRVLDVVSEKFGFSIQKEWAD
                     VGGAAYDNHGSPLPESTLKIAENSDAILFGSVGGPKWESLPPHLQPERGALLPLRKHF
                     KLFCNLRPARVYKELAAACPLRSDIVGNGFDILCVRELTGDVYFGQPKGREGSGPDEK
                     GFDTMIYSRYEIERIAKFAFEAAMLRGKKVASVDKANVLTTMVLWRDVVKEVAANYPE
                     VALEHIYVDNAAMQLLRRPRDFDVMLCPNLFGDILSDECAMLTGSMGLLPSASIAEGS
                     FGLYEPAGGSAPDIAGKGIANPIAQILS"
BASE COUNT         4187 a         2794 c         2443 g         4371 t
ORIGIN      
        1 gctggattac tggagatggt ttagactcta tttctaaagc attccaatgg gtaaatatta
       61 gacaagacat gaccaacttg aactggatta tgtctgctgg tttttatctt ggcaattgga
      121 aattttattt tgaacgtggt gaagtcatac accgaaatgt aaaattactt gatactcata
      181 gtctttatta taaaggctct atcttttgga gcaatcgctt tgtaaaagaa agattaggag
      241 taagcgttcg ttttgatttt cagtggtttg gagaccgata cgattgtgct gtaaataaag
      301 atggcgtgcc agaaattatt cctctaaata aatatttagc tcttaatttt gaagcgaaaa
      361 tgcagatttt atcctttgaa ttatactcaa gaattgataa cctaaatcat agtctatata
      421 agcccgcagc aggctatact ccagagggag tccgtttttc ttacgggatt atctggtctt
      481 tttcaaatta aaaaatttac ttctgatgct taaaagaagg gctctttaac ttataaattt
      541 taggccagtt ctttccagta atccataaaa aatcaccgtc tacagcgatc ccatttaata
      601 catctacgtt tcgatgagac tttcttattc tttggcttaa ttcagataaa tccaaataag
      661 cacgtacttt tccagagggt aaatcaatga ttgctataga atcggattgc caaatattgg
      721 catagagagt attccctact acttccagtt catttaagtt tttaatcggt ttatttccat
      781 ctaaaacgtt aatgacttga actactcgaa aatctcctgg agaaaggcga tacaattcat
      841 gagagccatt actcataagc aacgttccat tccaaaaaga aagcccccag ccttcagaag
      901 gtattcgaaa agagcctctt aattgaaaag gttttgcgtc ataaataaag ccttttctat
      961 ttttccaggt gagccaaaaa atgacatctc ctacttgaac cgatccttct ccaaaataac
     1021 gttcatctag agaaatagaa tctaatactt ggccagagag tgttttacga tataaaaaag
     1081 acttgccatg taagcctgta gattcaatcc attcttttcc attaaaattt agcccctgag
     1141 taaaatgaga cgtggaatga ggtaaagaat caacaatgct aaaagattga agagccgccg
     1201 caaaaaagag ggaaagataa atactcatac aagaattata gaataattgc tattatttta
     1261 catatgggcg cgataaaaaa acctctctta gtaaagttaa tcattggcat tttatgcggt
     1321 gaccccaagt ttatggatca tgttcgccct attctaagcc aaaaattcgg aaaagaagaa
     1381 gaaatccttc ctctatttcc ttttgttttt acgaattatt atgaagaaga aattggagac
     1441 aagcctctcc gcgcctttct ttcttatgag cgtttaattt ccagagaaga aattgtagat
     1501 ataaaacttt ggacgaacga tctagaactc gaaattgcaa aagaacaagg gactggggaa
     1561 aaacgcccca ttaatctaga tcctggatac atgactcttg gccaattttt cttagcaaca
     1621 acaaaagacc aacgtcaacg agtttatatt cgagatggta tttttgtgga acctactctt
     1681 tattttcaag caggtcattt tcatcctttt gattggacct acagagatta tcaaagcgaa
     1741 acttatattg agtatcttga aaaagtgcgg caaaaactag cgtatcaaca cgccacagga
     1801 aagccttacc gagaacgttc cactccatta aaataaaaac gacctatgct caaaactcta
     1861 ctcaatcctc atgttctcac tgttttaatg cttatttgct ccaactgttt tatgacattt
     1921 gcgtggtatg ggcatctcaa aaacatgtct cacaaagcat ggtatatagc cgcttttgca
     1981 agttggggaa ttgctctttt tgaatattta attatggtgc ctgcaaatcg ttttggctat
     2041 caagtatata ctcttcctca gctcaaaatc attcaagagg tggtcactct ttctgtattt
     2101 atcccttttg ctgtatttta catgaagcaa gattttaagc ttgattatat ttgggcttgc
     2161 ctatgtcttt taggggcagt ctattttatg tttagaaatt aacgattacg atttttcgca
     2221 aataaaaaag ccagaaagcc cctcgttttt ttataaaaac aagtccacta taatttttct
     2281 ttaacttttt ttgaaaagaa aagttaaaat agcctttgta tacctatgag gtataaatct
     2341 tggttaaatg aatcttcgaa aaaattgccg aaaaccttac attggcattt aaacatatta
     2401 gatatacaat gtcgttaata cattataatt tcacgcattt ttctcttttc gcgaaaaaag
     2461 tcaaaaagca cgagcattat acacccttta aaaaagcaaa accctgcctt tactaagaca
     2521 gggtttttaa tttcaattaa gctaagaatt aaccttttgc attaacccaa tcagcattat
     2581 tctttacagc agcaacagat ttatcaaatg cagctttttc agatgcatcc atagccactt
     2641 cgagaatctt ttctacacca ccagcaccaa taacaactgg aacgccgcaa tatagatcat
     2701 tcacgccgta ttcgccattc aatttagcag cacaagtgat gacgctcttc ttatcgagca
     2761 agtaagcttc tgccatttta attgcagaaa gacctgggct ataaaatgcg gaacctgttt
     2821 tgagaagttc aacgatttca ccgccagcat ttgctgtacg tacagcgagc ttatcaaaag
     2881 tttctttatc cattaattgt ggaagaggaa taccgccaac agaaacgtaa ttatataagc
     2941 taaccatggt atcgccatga ccacccatca caacagcgct aacatcttct gcagcaacac
     3001 ctaattccat agccacaaag caagctaaac gagaagaatc tagaacacct gccataccaa
     3061 ccacttgctt tgcagggaaa ccagtgactt tttgcattgc ataaaccatg gcgtctaatg
     3121 ggttagtaat aacaataaca aatgcgtttg gagcagttgc tttaagagct aaaccaactt
     3181 tttcaataat tccatagtta ataccaagaa gatcatcacg actcattcct ggcttacgag
     3241 gaacacctgc agtaacaata acaacatcgg aaccagcaag atcagcataa tcattagaac
     3301 cagagagctg tgtagaagag tttataacgg aacgcccttc cataatatcg agagctttac
     3361 cttgaggtac gccaggtact acatcaacaa gaacaacatc gccgagttgc ttttgagcaa
     3421 gggcgagagc catggtacca ccaatctgac ctgcaccaac gagagcaatt ttttttctag
     3481 ccataataag cttccttaaa aagaagttga gttgtcaaat gacgagtata tagatagcaa
     3541 aaattgaata aaaaatcact agctttttac taactttaaa cttatgacta caacaaaagt
     3601 acaacctcct caacttccca aaggaacccg cgatttctac ccagaagaca tgcggattca
     3661 aaactttatt tttgatactt ggaaacgtat ttctcttcgt tttggctatg aagagtacga
     3721 aggcccaatg tttgagcatt tagagctatt tacgggaaaa tctggagacg aaattgtaag
     3781 tcaactttat aattttgaag ataagggcgg tagagcgatc tccttgaggc cagaaatgac
     3841 gccaactctc gcccgcttag tcatccaaaa aggaaatgca atcaaaaaac catttaaatg
     3901 gttcagtatt ccaagacttt ttcgttatga aagagctcag aaagggcgtt tacgagaatt
     3961 tttccaatta aatttagatg ttataggcac cgaaagtatt tttgccgaag ccgatcttat
     4021 ttctgcaatt gcttccatgc ttttagagtt tggtcttaag cctactgatt ttttaattgg
     4081 tatttctagt cgtaaattac tctccactta tcttgaagaa atcgggactc caagcgctga
     4141 attagtctac cccgttttag accgccgcct caagataggg ccagaagctt ttgccgaagc
     4201 cttaactgcg attggattat ccgaagatca aaccaaaaaa attgattctc tcatgtgctg
     4261 taaatcaata gaagacgtac gaggacttgt ttcttctcca gcttctatag aagctcttaa
     4321 agaaatagaa gacttatttg acatattaac aagcactggt tttggtgaat gtattgggct
     4381 tgatctaagc atcgtccgtg gccttgctta ctatactggc attgtcttcg aagtctttga
     4441 taaaggcaaa tctatgcgcg ctattgctgg tggtgggcgt tatgatagtc tttgtgaaaa
     4501 actaggtgga gctaaagttc ctggtgtagg ctttggcatg ggagatgttg ttcttgcaga
     4561 tcttcttaga gaccacgact tactccccac acagaaacag actcttgatc tttttattgc
     4621 ttcttttact ctcgatatga agaagatttt caaaacggca aatttgttca gacaaatggg
     4681 ttttaatgta tctcacccat tatctccgat aaagcttggc aaacaaatgg aacttgctaa
     4741 tcagcaaggt gcttcctgcg tggtttatgt ggatggagaa aatgctaaag aaggtgaatt
     4801 tgagtttaag gacctaatca aaggtactat gaatgtgggc tctgctcaaa aaatttcgga
     4861 atacttaaga taatgagtcc ttttaaaact ctttgttcca ttattattct ctttgtagcg
     4921 ttaggacttc tatcagtcgt atttccagaa aaaggaattt ccttttttaa cgttactctt
     4981 cggtttccga cactgtctga tatgtttcca aaggctgaag ataagcctac tgaaaaagcc
     5041 gaagatccag aaatagccat tcagagaatg cttgaagaaa cacgtcaacg tgagttttcg
     5101 gtctatgctg attctctcca cttttatgaa tccttcttta aaaaaggcgc cactcgtttt
     5161 gatttgccaa acaacgaccc tacttggttt gatcgtttct ttttacacct agaactagcc
     5221 tctcttgaag aaaaagtggt tcacattatt cattatggcg attcacaaat tgaagaagat
     5281 cgcatttcag gaaccattcg agaagagttg caagaagttt ttggtggaag cggtccagga
     5341 atgctccccc caatacttga agtaccttct atgacaacaa gtcattggaa ttcaggtgat
     5401 ctagaacgct ttattttatt tggcccaaaa gaacaagaag ctacccataa tcgttacggg
     5461 cctctcgccc aatttgccaa tctcaacgga tcggcaagca ttcaaattaa gaaaaggccg
     5521 aatagaaaga aacaatttcc tcatgtagga gagtattcac aaatacgtgt cttaactggt
     5581 aaagaaaaaa gtttgaatat ttctctttct tatgattcca cttatattga aaatgaaggg
     5641 gatcctgaaa aagagaagaa gaagaaaaaa gctgtcaatg caccagcacc taaagtcgaa
     5701 cgttttgaaa agctcaatgt ataccaatgg atacttccag agcccacaga tgtggcccgc
     5761 cttagtttat ctggagaagc ggaaatatat ggtatcgccg tagatgggcg atttggtgta
     5821 gctctagata acgtgccgat gagaggaagt tctggtacta tatttcaccg cattgacaag
     5881 acattgttaa aagaatctta tgacgcctta aatgcgcgct tgattattat ggaatacggt
     5941 ggtaatctcg ttcctggaac aaatagaggg aatgtggaat ggacgaaaaa aattatcact
     6001 cgtcaaattc atacaattca aagtgttaat ccaaatgccg atattttatt cattggccct
     6061 gctgatatgg caaaacaaat taacggtcaa ttacaaactt atccagggct tccacttgtg
     6121 attaaggctc ttagagaagt ttgcgaggaa aatggcgtag cctattggga tatgcaccga
     6181 gtcatgggtg gtaatggttc tatgcaaaaa tgggtaaaaa gaactcctgc cttaggccac
     6241 acagattaca ttcattttac ccgtcaagga gccgctcata ttggaaacct ctttagtagt
     6301 tccttaaaaa catactatga ttttttcaaa ttccgcgatg cacataaaat tgatgataat
     6361 aaattaaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnac tatgattttt
     6481 tcaaattccg cgatgcacat aaaattgatg ataataaatt aaacgatatt cgagcttttg
     6541 ctgattctct aaaaacacaa gattcgacaa ttaaaagctc tgcgataaca caggaggaac
     6601 aatgaatcga tggataggcc ttcttctttt gttctctagc ctcttagcgg caaacgagaa
     6661 caccctagat tttgcaaact atacatttat tgatacctct atcaacgaaa ttcatttccc
     6721 agcaggagat gaaagttaca agcattttta cgaaaaaatg gattctcttg ttttcgaaaa
     6781 taaaggtcaa gttcgaattg ttcactttgg cggatctcat atacaagcgg atgtcttttc
     6841 aaaccgtgtt cgcgaaaacc tagtgaaaag atatccaggg ctcgccgctg gtcgaggctt
     6901 tgtgttccct tattctgctg caagaacaaa taccccatct agttatgcct cctattataa
     6961 aggcaaatgg gatatgagca aaaatgtttt aaaagaaatc actaaaccct tgggcctttt
     7021 aggaattgca gtaagtacaa cagatccacg agcagaaata tctatttttt taaatcagta
     7081 taataatacg cccatttggt tttttaacga agtacgagtc tttggttttt ctgaacttaa
     7141 cgatgttgtt cccattgtca agttagattc tacggtatat caagggattc ccgacacagt
     7201 aaatcaatcc tttgttttta atctaccacg atccacagac tccctgatta tctcttttcg
     7261 ttggacggat tcactaaaac aagattcttt aattcaagta tttaaacagg attccatttt
     7321 acaagattca ctttcaaaag attctcttat aaaagattcc ataagcaaag gtgtttctaa
     7381 aacagatgta gtctctgccg attccatgtt tcaagactct ctgattatta gcgataccat
     7441 acctgattta catcacaaat ttactcttac tggaattctt gtagaaaatc aacacccagg
     7501 catttcctat acaaatgtgg gtattaacgg agcaaaagtc caagcctatt tagtttgccc
     7561 caatttagaa cgcgatttat ccttttttaa accagacctg attatttttt ctattggtat
     7621 taatgatgcg aacgttgaac attttaacga taaaaaattc caagaggaat acgactcctt
     7681 aataacttta atacaaaaag tagctcccaa ggccgcgatc ttatttacta caaacaacga
     7741 ttcttataga aaagtaagaa ggaatcgtta catccaacat ccaaacggca aagtggcaag
     7801 agaagccttc cttaatatgg ctcaaaagca taaagccgga gtatgggatt tatttaccat
     7861 tatgggcggg cttggctcca tggcaaaatg ggaaaaagca gaccttgcta aaaaagataa
     7921 agtccatttt aaaagttctg gttacaaatt gattggggat ctattttatg aagcccttct
     7981 taaaagttat aacaatcatg tggcccattt accagcagaa aagccttctt tagaaaagat
     8041 agaaaaaggt actaaaccat gattgatcgc ttgatttctt atttctcttt gattttttct
     8101 tttgatcaaa acgctccttt gctctttaca caattttatt tctgggcttt ttttgccatt
     8161 gtttttgctg ttttttcgtt aatccataat aaatttgcac ttcgcaatgc cttcttgttc
     8221 tttgttagtc tcttcttcta ctataaaaca agcggaagtt atgttcttat tcttattttc
     8281 actgtagtta tcaacttcta tctcgccaaa tggattcacc gtaacacctc catttcatgg
     8341 cgtcgatttg gagttatttt agcagtcatc gttaacctac tcaccctgag ttatttcaaa
     8401 tacacgtact ttttcttaga tcttattcaa caagtctttg gaatcgaact acacgcttat
     8461 aacttcttta acgccgccag taattatctg tttaaaacag aatctttagt cgatagaatc
     8521 gttcttcctg ttggtatttc cttttttact tttcaggcca tcagttatat cgtcgacgtc
     8581 tatcgaaaaa ccgtaacccc agtaaaaaaa ctattagact ttggatttta ccttaccttt
     8641 ttcccacagc tcgtcgctgg cccgattgtt cgcgccaacg tgtttattcc ccaactctat
     8701 aaacaatcgt acttacctcg ccgtactttt ggcttagctg ttttttggat attaaatggc
     8761 cttgccaaaa aaataatcat gagcgattat cttgccgtga attttgtaga ccgcgttttt
     8821 gaaacaccac tactctttac tggatttgaa aacttacttg ccttatttgc ctattctctc
     8881 caagtgtatg cagacttctc tggttatacc gatattgcga ttggtgttgc cctactgatg
     8941 ggttttcatc ttcctaaaaa cttcaactca ccatacaaag ccataaatcc cactggattt
     9001 tggcgtcgct ggcacatgtc cttatccact tggttacgag attatcttta cattccacta
     9061 ggtggcaatc gccgtgcagg cttcggtact tttttctgga ttgccattat ttccatcatc
     9121 gctaccattc tttcgggtag ccttattgtg ggtttagttt tagttctgtt ttttataggt
     9181 ttagggatat acgcttactt ccatgcagaa gacaggcgct cgatcaccac acaaatgaac
     9241 aacatgacta caatgcttct cggtggtctt tggcatggtg ctagctggaa ctttatgatt
     9301 tggggtggtc tcaatggagc aggtatgatt ttaaaccgct tgtggtgccg tttccaaacg
     9361 cctgtgagag ctatactcat gtttgcgatg accactactt ttataattct ctccaaacac
     9421 ttctttcatc cactctttgc cattctagcc gtttggggtg gtctcatttt tgttggatta
     9481 tacttcaatt taatctttag actttttaca gataaaacca ttaattgggt gtaccagtct
     9541 tggaatattc ttttaacgtt tatttttatc acattcactc gtttattctt tagatctggt
     9601 tctaatttgg atccagtgga agcaaacgaa acggcctggg aaacagcgac caatatgatc
     9661 cgccaaatag gcagtccatg gaatctagat actgtcccaa cgatgatttt tgaatataaa
     9721 aacatcattt tagtatttgc tctagggatg attatccact ggcttcccga tcgctttaag
     9781 aggctttatc gctatgtatt tgcaaacttc gcactcccta tacagatcat tctcacggct
     9841 ctatccgtat ttttgattta tcagtttatg agcgcagact ctcaaccgtt tatctacttc
     9901 caattttagc ctaaactatg tataaatcaa cattcctgtt ttttattcta ttggctgcaa
     9961 gattaacaca gttagtgcaa ttgtacttta catctattcc ctctggcggt ttactaacag
    10021 aacagcccat taaatctttc atatgtgcat ccattgtaga aatcagtact ttcgccatca
    10081 tagctttttt gtttagtatc gtatttctga ttaagcaaaa aaaatggcat tctttctttt
    10141 atgctttttt tgcctccatc ggtgttatct atttactctt ctctgcaatc aatgaccaag
    10201 tcatgcgatg gatggggcaa cattttgatc tagcctttat taaaacctat tccgtatcga
    10261 atcttgaacc aaaactcttt ctgaatatag catcaaacgg cattgtcagt tttattattt
    10321 cttctctcat tgtgctcatc acagcatggc ttctctattt tatccccaaa aaaatcactt
    10381 tcaagaatca ttcttatttt tcgttaggtt tatcttctat tgttctattc gctcttttta
    10441 ttatcagttt tactgccatc gattggtata aacctgtccg tattcgctgg aaacaaatcc
    10501 aaccgattgt agcgacttgg tattatgata taaaatacga tttaacgcat aaaaataaaa
    10561 ctgaagatat tttaattggc attgaagaat tggggggaga tataaatagt gattacccct
    10621 tctatcattt tgtcgccaat gaagattctt ccctacaagc atttaaatct aaacctcttg
    10681 aagaacgtcc tgatatcatt ttactgtcac tagaaagcct gcgcggctgg gctagtgatt
    10741 ttcgtatttc agaaaattgc agtcgcatgc caaacctatg tgctctctct aattcaggaa
    10801 catcattccc ttatacatat agtgttggat tcccttcaac tgaaggcatg actggtctac
    10861 aattaggtat atggagccat cctcaaaaag tattcctcac tgattttatt tcttcaaatt
    10921 caagagcttt gccagaaatt ctaggtgatg caggatatta tagacttttt ttgaccgccg
    10981 ccgaacctag ctttgataat ttcacaccat ggtttaataa atgggccgat tatcatgaat
    11041 ataaaaaaga aaatgattca gacattcctc ttgcccatcg atttgtagaa ctctataaaa
    11101 aacgtccaaa agacaagcct ctttattcca tttggatcaa ctttactaca cacactcctt
    11161 ttaccgttcc aaaatcttat gcgagcccag ctaatacttc tgaagaacgc tacaaacaag
    11221 ttttaactta tttggattcc gcgcttggta ttatttttaa ggcaattcaa gaagacgatc
    11281 gccatcatga aaccatccta gtcattactg gagatcactc cattgccaat aacgccagtc
    11341 aaaaagcaag tagccaaaat ggaaatgctt tttctggaca gacatggacg acgatgactt
    11401 ggtacggcta tcgtgttcct aaagaaaaga aaattcttag tcctatatcc catgtcgact
    11461 ttgcaccgac gattctctcg cttttagatt tatccgtctc taatcatttt gttggcactc
    11521 cactttttga cgaagaaaac gttcaccata ccgtttatag tttccgtcgc ggagggattg
    11581 ctagaagaca agactctatt atttgcttca cagatgtaga tccatctgtt ccttcgctat
    11641 acaaaaaaca caattcgttt attgattggg acactacaaa cacaattgaa ggatttgctt
    11701 ctgagcccga tttagaaagc cctcatgatt ttaataatct tgaacatcgt gcaagagcag
    11761 ctgcagaatc ttggagatat attttagatc ataacaagct tttccccact cagaaataat
    11821 cgataaacct agcttgaatt caaaaaccat aaaaaaaacc tctgtttttc aacagagatt
    11881 tttcgtggat gatgcaggga tcgaacctgc gacccgctga ttaagagtca gctgctctac
    11941 cgactgagct aatcatcctt aagtgctttc aattataaaa taacttttgt acattttcaa
    12001 gataaatttc tttacttttt tcaattttat catgacaaac ggttcacttt tagacctcta
    12061 ttgtagtcat ctcagtattg agcgaaactt atcttcggca acggtcagaa gttaccaaga
    12121 agatttacgt cattttattt tttggctcga tgatcatgat atctcactaa gagaccttga
    12181 tcttcccctt ttagatcaat ttcttaatca attggcctta aatgtcgaat atagccccac
    12241 tcccattgca aggcattttt cgagtttacg tggcttctta aaattcatgc aacaagaggg
    12301 ttactactct cattccacag aatccatgct cgcagttccc aaaattggac tttaccttcc
    12361 tcaatgccta aatttagacg aagtggatag cgtttttaag catattactg aattttcaaa
    12421 ataccctctt cgagataccg ctctttttga acttctttat agcgctggac tacgtatatc
    12481 agaagcctta aatctaaagc tagaacacct agatctcgaa aatgaatggc tcactcctgt
    12541 aggaaaaggg aataagcaac gattagtgcc ccttggttca aaagcgaaaa tgaaccttca
    12601 aaaatggatc aaagaaggtc gtccagagac agaacccaaa agtgattgtg taatcttaaa
    12661 tctaaagggt cgccccatga gtcgaatggg agcctggaaa atagtccaac aacataccgc
    12721 ccacttaaca aagcacgttt cgccgcatac ttttaggcac tcttttgcca ctcactgcct
    12781 cgaagcagga atggatttaa gggttttaca agagcttcta gggcacgctg atattgcaac
    12841 aacgcagatt tacacgcata ttgataaaga gtttattcaa agagaacacc acctacacca
    12901 ccctagagaa ataaaagcta caccgaatga ctagtttttt gctagattat gtttgaattt
    12961 ttattgtttt ttaaagagga atgtatgagt aaaaattata aaatagcggt tcttccaggt
    13021 gatggaattg gcccagaagt gatgaaagaa gccgttcgtg ttttagatgt tgtttctgaa
    13081 aaatttggat tttctattca aaaagaatgg gctgatgtag gcggtgctgc ttatgataat
    13141 catggctctc cacttccaga aagcacttta aaaatcgctg aaaatagtga tgctatcctt
    13201 tttggttctg taggcggccc taaatgggaa tcccttcctc ctcatcttca accagaacgt
    13261 ggtgcgttac ttcctctaag aaagcatttc aaattattct gtaatctacg ccctgcacgc
    13321 gtttataaag aactcgccgc agcctgccct ttacgttctg atattgttgg aaacggtttt
    13381 gacatccttt gtgtacgtga attaactgga gatgtttact ttggtcaacc taaaggccgc
    13441 gaaggctctg gaccagatga aaaaggcttt gacaccatga tttattcacg ctacgaaatt
    13501 gagcgcattg ccaaatttgc ttttgaagca gccatgctcc gcgggaaaaa agtcgccagt
    13561 gtagataaag caaacgtttt aaccaccatg gttctctgga gagacgtggt aaaagaagta
    13621 gcggcaaact accctgaagt cgcactggaa cacatctatg tcgataacgc cgctatgcaa
    13681 cttttacgtc gtccacgtga ttttgatgtg atgctttgcc caaatctttt tggcgacatc
    13741 ttatctgatg aatgtgctat gctcactggt tctatgggtt tactcccctc tgcttctatt
    13801 gccgaagggt cttttggtct ttacgaacct gcgggtggtt ctgctccaga tattgcaggt
    13861 aaaggcatcg caaacccaat tgcacaaatt ttatc
//
LOCUS       DAOOBB010000032        13834 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_5706_length_13834_cov_4.884825, whole genome shotgun sequence.
ACCESSION   DAOOBB010000032 DAOOBB010000000
VERSION     DAOOBB010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 13834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13834
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5706_length_13834_cov_4.884825"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="PLT31_02710"
     CDS             complement(<1..865)
                     /locus_tag="PLT31_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HPW94078.1"
                     /translation="MGEKVSLIYKDKTYEYPVITGTENEKAFDTSCLRKETGLITMDY
                     GYQNTGSTKSSITFVNGEEGILRYRGYPIEELAEKATFPETAWLLIYGELPTEAQLTV
                     FRRLLTENSLVHENLLHFFREMPPSAHPMGILSSIVNALGLFTPRFYDAESKADEFDL
                     MVAGLVSKIRTIAAFSYKASIGEPFVYPEADRSYCGNFLNMMFSSHTKPYVVNPLMEK
                     ALNALLIVHADHEQNCSTSTVRMVGSSQANLYASICAGICALWGPLHGGANQAVLETL
                     LHVVPSKLIVMI"
     gene            complement(858..2159)
                     /locus_tag="PLT31_02715"
     CDS             complement(858..2159)
                     /locus_tag="PLT31_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPW94079.1"
                     /translation="MILHPLAQALNQDLSAEGCSILDTLSSQGKAIFFPSKGILGQGA
                     EAKDAAINATIGTALEDDGSALTLPCIQKSMNLNKSSFLYAPSFGDPKLREAWKAQIQ
                     KKCPSLGNTKISNPVVTAALTHALSCAAYLFLDPGDEIIMPDLYWDNYELVFENGRGA
                     KIKTFNTFVNGGFDVEALRSALQNSKSSKKVVLLNFPNNPTGYTATDEEASAIAKVLK
                     ESADAGNSVVALLDDAYFGLVYEQGVYKESLFSKLADSSPNLLAVKLDGPTKEDYVWG
                     FRVGFVTFAFKGATDAQLKALENKIAGVVRGNISNAPSISQKILLAAYTCNEYEVEKK
                     EKYATLKKRYDIIKEVLASHPEYKKTFTPMPFNSGYFMCIRPIGIDAETIRKDLIANY
                     STGVIVLSGLIRLAFSAVPTGKIPLLFDNIYNAISKRQNHG"
     gene            2539..3561
                     /locus_tag="PLT31_02720"
     CDS             2539..3561
                     /locus_tag="PLT31_02720"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HPW94080.1"
                     /translation="MQEIDWKNIPFGFSETDYNVRCYYRNGSWGKIEVSSSNTINMHM
                     AATSLHYGQEGFEGLKAYTGKDGQVRIFRCEENAKRMQRTAERVLMAPPPVELFHEMV
                     KQVIQLNRRFIPPYGYGATLYIRPLLIGTGAEIGVRPSKEYLLIMFVMPVGPYFKDGF
                     KPVDMMICRDFDRAAPLGTGTVKVGGNYAASLMSLEIAKQKGFSSTIYLDAREKKYID
                     ECGPANFFGIKGKKYITPKSESVLPSITNMSLQQLATHLGYEVERRPVPFEELAEFDE
                     TAECGTAAVITPIKRIVDPVAGKEFTYGNGDAGPVCTHLYKEYTAIQFGESPDPFGWT
                     EVIDFK"
     gene            complement(3738..>4370)
                     /locus_tag="PLT31_02725"
     CDS             complement(3738..>4370)
                     /locus_tag="PLT31_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPW94081.1"
                     /translation="NVMHHSQKNVLRYIIKRTVDLSCAIIALLLLSPLLLIIALAVKI
                     SDPKGPIIFKQTRVGKNGKLFTMYKFRSMYADAEERKAALQEYNESGGPTFKMKNDPR
                     IYPFGRILRKFSLDELPQLFNILKGDMSVVGPRPPVPAEVAEYLPWHKMRLSVTPGLT
                     CFWQVSGRSNVTFEEWMRLDNQYVRHGNFGVDAQLIAKTFKVVIKGDGAY"
     assembly_gap    4371..4470
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4471..4770)
                     /locus_tag="PLT31_02730"
     CDS             complement(<4471..4770)
                     /locus_tag="PLT31_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPW94082.1"
                     /translation="MSNDSTKNHLDSLISSQNLGNPIYPAKLFQARLKEEFLRTNRIQ
                     RPFLYIKIFARQFDIFGWAKPDKVVLRTWNICVLSMLSQTKFTDVVGYLSEGSGLG"
     gene            4874..6154
                     /locus_tag="PLT31_02735"
     CDS             4874..6154
                     /locus_tag="PLT31_02735"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HPW94083.1"
                     /translation="MRIGLIGTGTVGGGVIDILESKSKSFQEDLGLTLELACICARTD
                     EELAPFKAKGYTVTTSADQMIKDPSIDVLVELAGGYEMPRKWITAALNEGKHVVTANK
                     ALLAKYGHELFPLAEEKGAHILFEAAVGGGIPIIRSIQEGFVGSKIEHLSCIINGTCN
                     YILTRMSKEGLSFESVLKDAQAKGYAEADPTFDIEGVDSAHKTALLASLCSRKHVDFE
                     KIHVTGVSSITSADIAYAKELDCSIKLLGLYHASGDRVDARVHPCLVPNDHLLSSVNG
                     VLNAVFLECDNLGPTLQTGAGAGRLPTASAVVADLVSLARSAGKRESIPMGWFQKNNE
                     ADLVPIEETEARYYFRFTTQDACGVLANITSILAKNQISIESIIQKDVKDPGKVSIVV
                     ITEKTKEKRISSAIASIDALESVSDKSQVIRFLM"
     gene            6170..7621
                     /locus_tag="PLT31_02740"
     CDS             6170..7621
                     /locus_tag="PLT31_02740"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPW94084.1"
                     /translation="MFRATTLERTLLVISDFIAFSICFVIAFWVQFYSGWIADKLDVS
                     KQLLDYLPAGVLLSFAWIIFFFLTGLYRSWLLLSRTQQALYVLRSILFGVVFLIVGLF
                     GSEFIGKIMASAPLTEGYLYGSRFIWIFAYGLLAFFLVVFFRMLIYLFLRRLLRQGFG
                     ANRMLVLGATEMGAHVADSFMASPERGQKVIGFVDERYQVLPKEYANLPLLGKYSDLP
                     RIVRENHVRGIIISHDSTSVQEIMRILVWLCDLPVHIYIIPNLYNVVHGHFKANLVHG
                     FELQELFPFNMPLWQVRVKRAMDIIIAFGLLILSSPIFFLAAIGVKLDSKGPIFYSQE
                     RVGLYGRRFMVHKFRTMKVDAEKNGAQWATKNDPRITRIGLFLRRARIDELPQLLCVL
                     KGDMSMVGPRPERAVFIEQLREQVPFYISRLKIKPGLTGWAQVRHHYDTSIEDVQKKL
                     QYDMYYYENMSLLLDIQILFRTIYVVITGKGAQ"
     gene            7626..8489
                     /gene="nadC"
                     /locus_tag="PLT31_02745"
     CDS             7626..8489
                     /gene="nadC"
                     /locus_tag="PLT31_02745"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HPW94085.1"
                     /translation="MSDPKLLESFPVADAVTLIRLALKEDVRTGDITSAWTIPPEQKQ
                     KAKLIAKESGVVAGLSIIDLVFQELKETVSVTLYKQDGDAVVPGDLIAELSGTTRALL
                     TGERTLLNFIQQLSGVATITSRYVDALKAGSTHVLDTRKTIPGFRTLQKYAVRAGGGL
                     NHRMGLFDMVLVKDNHIAACGGVLEALAVVKKNNTSCVRVEIEVENLEDLKRLLHQGV
                     DVIMLDNMDNETMAKAVQIVRESGDKVQLEASGNMNLERLREIAAIGLDFVSVGALTH
                     SVKALDISMRI"
     gene            8489..9301
                     /locus_tag="PLT31_02750"
     CDS             8489..9301
                     /locus_tag="PLT31_02750"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HPW94086.1"
                     /translation="MTKGVILVVDVGNSHTVVGIFRGDKVVDHWRLTTRPNTTSDEVL
                     NRVSGLLRFSEIKPNQITHIGLSTVVPAHERPWIKALTFLFNKPVQVVSSDNCLKLPI
                     AYLNPSMAGSDRLCNVIALRDRGYTDAIIVDMGTATTFDVMKDGGFAGGLIIPGISAS
                     LDVLTEKAARLLPVTIEWPENLIANNTDDAIRSGLLFGFKVELESIVALIKKEFTKEN
                     IPVFATGGWGKTIARRTECIDTYDPYLTLNGIRLVALYGEGAVVVDEEGDDE"
     gene            9298..10239
                     /locus_tag="PLT31_02755"
     CDS             9298..10239
                     /locus_tag="PLT31_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPW94087.1"
                     /translation="MRILVTGGAGFLGSHLCERLLSSGHEVICLDNFFTGRMDNIAHL
                     RDITRFEVIRHDVTEPILLEVDLIFNLACPASPVHYQHNPVKTIKTSVMGAIHMLGLA
                     KRVHARILQASTSEVYGDPTQHPQRESYWGNVNPIGIRSCYDEGKRVAETLFMDYHRQ
                     NKVDIRIVRIFNTYGPRMLANDGRVVSNFIVQALQGQDITIYGSGQQTRSFCYVDDLI
                     SGFVSMMDQTQTVGPINIGNPSEFTMLQLAEEVIRLTGSKSKIVYQPLPGDDPQKRRP
                     DISKAKEILNWEPVVSLEEGLIKTITYFDRLIEAGIR"
     gene            complement(10247..10861)
                     /gene="nth"
                     /locus_tag="PLT31_02760"
     CDS             complement(10247..10861)
                     /gene="nth"
                     /locus_tag="PLT31_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HPW94088.1"
                     /translation="MNRTERIKFIATKLDEYFPNPPIPLIHEDAYTLLIAVLLSAQCT
                     DVRVNQITPFLFKKAKTPEAMIKLKVETIMEIVKPCGFFRTKGTNIFNLSKILIEKHQ
                     GQVPRSFEELEALPGVGHKTASVVMSHAFKIPAFPVDTHIHRLAKRFGLSNGSSVEQT
                     EKDLKKIFPKEEWEKRHLQFIYFGRNFCKARGHVVSECPICKGL"
     gene            complement(10890..11690)
                     /gene="cysE"
                     /locus_tag="PLT31_02765"
     CDS             complement(10890..11690)
                     /gene="cysE"
                     /locus_tag="PLT31_02765"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HPW94089.1"
                     /translation="MTISEIEKELIKAAKELVSKEALSTNILNQQILSKSSFGEMLAA
                     TLAQLLGGDLVSKKDLEVLFLTQFRKYPSILTNAASDLEATKMRDPACSNILDPVLFF
                     KGFQGLQAYRTAHTLWNEKRTFPAKMLQSIISQKFGMDLHPAAKIGCGILIDHATNIV
                     VGETAKIGNNVSIFHGVTLGGSGNETGDRHPKIGDGVMLGAHAQLLGNIRIGRGAKIG
                     AGAVVLKNVPPHTTFAGVPAVQVGHPENDMPSFEMQQDFTLDAKLDPK"
     gene            complement(11723..12085)
                     /locus_tag="PLT31_02770"
     CDS             complement(11723..12085)
                     /locus_tag="PLT31_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94090.1"
                     /translation="MEIVLEIAPQKATHLKKRLAVLEQYFRDGSLLLSARDGEKPAQF
                     ILHSKDSFPWLAFFKKLRVFWWASSHQAIPYAFRLQKRVPEELLAELDLLSEPELLDA
                     LAALRANKYFPPLPKSPF"
     gene            complement(12106..12246)
                     /locus_tag="PLT31_02775"
     CDS             complement(12106..12246)
                     /locus_tag="PLT31_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94091.1"
                     /translation="MSNNEYAKINYARALIRAGVRKELILKITSISTYQYSLIERELQ
                     VA"
     gene            complement(12412..13221)
                     /locus_tag="PLT31_02780"
     CDS             complement(12412..13221)
                     /locus_tag="PLT31_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HPW94092.1"
                     /translation="MAKVYLIQMDILWCKKTQNFETVRRLLKSHPIEKGGLIVLPEMF
                     ATGFVGRPKDGIEESFDHSESGITASFLYELAQETGCWVQGSGISKKNGRFRNHISVY
                     SPDSTVEITQYDKIYPFHTEQKHFEAGNEITTYQFDSFKVAPFICFDLRFPEVFRAAM
                     NYNIELFTVIASWPSIRESHWKTLLKARAIENQCYVLGVNRIGEDPYTKYNGGSMIIA
                     PTGEVIASLDSKEGVVSAEIHYDFLAKVRQNFPVLNDAKPFFSFPIKNNVI"
     gene            13392..>13834
                     /locus_tag="PLT31_02785"
     CDS             13392..>13834
                     /locus_tag="PLT31_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94093.1"
                     /translation="MRSILFLLTASIMLIAAPTRLVGVVLEAGSDRPVADVNILYMSG
                     KKISSTDARGRFDIEVTSENTVLLVKKEGYDSLTVNVSEFPDFLDMVILVSTNVRDLG
                     QSTVIGGENIVEWENVRHVELDKLEDAAGMRFDLTEHLSQMSGISG"
BASE COUNT         4074 a         2460 c         2842 g         4358 t
ORIGIN      
        1 gaatcattac tatcaatttc gatggaacaa catgtaacaa tgtttctaaa acagcttgat
       61 ttgcaccacc gtgaagaggc ccccacaaag cacaaatacc agcacaaata ctcgcataaa
      121 gattggcttg agaactaccc accatacgaa ctgtacttgt agaacaattt tgttcatgat
      181 ctgcatgaac aatcaagaga gcatttaatg ccttttccat taatggattg actacataag
      241 gcttggtatg gctagagaac atcatgttta agaagttacc gcaatagctg cgatcggctt
      301 ctggatacac aaaaggttca ccaatacttg ctttataaga gaaggcggca atagttctga
      361 ttttagaaac aaggccagcg accattaagt caaattcatc tgctttgctt tctgcatcgt
      421 aaaaacgagg tgtgaaaagc cctagagcat tcacgataga gctgagaatt cccatcgggt
      481 gtgcagaagg aggcatctct ctaaaaaagt gcaataaatt ttcatgaacg agagagtttt
      541 cagtgagcaa gcgacgaaat accgttagtt gggcttccgt agggagctct ccataaatca
      601 atagccatgc ggtttcagga aacgttgctt tttcggcaag ttcttcaata gggtaaccgc
      661 gataacgtaa aatgccttcc tctccattca caaaggtgat gctacttttg gtgctaccag
      721 tattttgata cccgtaatcc atagtaatta aaccagtctc tttgcgaaga caagaagtat
      781 caaaggcttt ttcattttca gtccctgtaa ttacagggta ttcgtacgtt ttgtctttgt
      841 atattagaga aactttttca cccatgattt tgccttttgg aaatagcatt ataaatgtta
      901 tcaaataaaa gtggaatttt accggttgga acagcagaaa atgcgagacg gattaaacca
      961 gacaaaacaa tcactccagt gctgtaattt gcaatgaggt cttttctaat cgtttccgca
     1021 tcaataccaa tggggcggat acacataaag taaccactat taaaaggcat tggtgtaaat
     1081 gtttttttgt attctgggtg agaggcgaga acttctttaa tgatgtcgta tcgttttttt
     1141 agtgtggcgt atttttcttt tttctctact tcgtattcat tacaagtata agcggctaaa
     1201 agaatctttt gagatatgct aggggcgttt gaaatattac cacgaacaac accagcaatt
     1261 ttattttcaa gtgcttttaa ttgagcatca gtagccccct taaaggcaaa ggtgacgaat
     1321 ccaacacgaa aaccccaaac atagtcttct ttagttgggc catctagttt tacagctaaa
     1381 agatttgggc ttgagtctgc tagtttagaa aataaagatt ctttataaac accttgttcg
     1441 tacacaaggc caaagtaagc atcgtctaat agagcgacaa ccgaattacc agcatcggca
     1501 gattctttaa gaactttggc gatggcacta gcttcttcat cggtagcagt ataacctgta
     1561 gggttattag ggaagtttaa tagaacgact tttttagaag atttggaatt ttgaagagcc
     1621 gagcgaaggg cttcgacatc aaatccacca tttacaaagg tgttgaacgt tttaattttc
     1681 gcaccacggc cgttttcaaa gacgagctcg tagttatccc aataaaggtc aggcataata
     1741 atctcatcgc ctggatcaag gaataaataa gcggcacaag aaagggcatg cgtgagggct
     1801 gccgtgacga caggatttga aatttttgta ttccctaaac taggacattt tttttgaatt
     1861 tgtgctttcc aagcttcgcg aagtttagga tctccaaaac taggggcata taaaaagctt
     1921 gatttattta gattcatcga cttttgaatg caaggcaatg ttaaggcgga accatcatct
     1981 tctagagcgg ttccaatcgt tgcattgatg gcggcatctt tagcttccgc tccttgtcct
     2041 aaaatccctt tactggggaa aaaaatggct ttcccctgag aggaaagcgt gtccagaata
     2101 gagcatcctt cagcagaaag atcttgattg agggcttggg caagtggatg aagaatcatc
     2161 tgaaatccta tgtaggttaa aaggtgagca aacgcctgaa taataaaaaa aataggagct
     2221 tccttgtatc cccctgaaca aaaaaaagag aataataata aaggatttat ctaattcggg
     2281 gatgggatgc aatactttgc aatcgtttgt cataaaaaat ggtggaatca agattaagtg
     2341 taactaaaag acttcatgag tcaagtgctg taacttggtc ctattttata taaggatgga
     2401 ctgaaatact tgtgattttt gtaagaaata acttcagaaa caattttttt ctcatttttt
     2461 aacccctctt ttgccaaaag aatggggata aatgtatttt ttaaccattg tttcattctt
     2521 ttcaggtagg catcttttat gcaagaaatc gattggaaaa acattccttt tggcttttca
     2581 gaaacggatt ataatgttcg gtgctattat cgcaatggtt cttgggggaa aatagaagtc
     2641 tcttcgtcta atacgattaa tatgcacatg gcagcaacaa gtcttcatta cggtcaagaa
     2701 ggttttgaag gtttaaaagc atatactgga aaagatggtc aagttcgaat tttccgttgt
     2761 gaagaaaacg caaagcgtat gcagcgtacc gctgaacgtg tattaatggc gcctcctcct
     2821 gttgagcttt ttcatgaaat ggttaaacag gtgattcaat tgaatcgtcg ttttatccct
     2881 ccttatggtt atggagctac tctttatatt cgtcctcttt tgattggtac gggcgctgaa
     2941 attggcgtgc gtccttcaaa agaatatttg ttaattatgt ttgtaatgcc tgtgggccct
     3001 tattttaagg acggttttaa gcctgtggat atgatgattt gccgtgattt tgatcgcgct
     3061 gctcctctgg gtacaggaac ggttaaagtc ggtggtaatt atgccgctag cttaatgtct
     3121 ttagaaatag ctaagcaaaa aggcttttct tcgaccatct atttagatgc tcgtgagaag
     3181 aaatatattg atgaatgtgg ccctgctaat ttctttggaa tcaaagggaa aaagtatatt
     3241 actcctaaat cagaaagtgt tttgccttcg attacaaaca tgagcttaca acagcttgcc
     3301 actcatttag gctacgaagt cgaacgtcgt ccagttccat ttgaagagct cgctgaattt
     3361 gatgaaacgg ctgaatgtgg tacagcagcg gtgattaccc caatcaaacg cattgtggat
     3421 ccagttgcag gtaaagagtt cacatacggt aatggagatg ctggcccagt ctgtactcat
     3481 ctttacaaag aatatactgc aattcaattt ggcgaaagtc cagatccatt tggttggaca
     3541 gaagtgattg attttaagta attctttaat taaagttcac aaaataagaa accctaaatt
     3601 gatgatttag ggttttcttt ttttgaattg ttttgagtcg atataacgag aaaaattact
     3661 taaggcgttg tcgtctttga ttaatagacg ccgttgctta ataagcccca tcgcctttaa
     3721 tcaatagagg aaatcgttta ataagcccca tcgcctttga ttacgacttt aaaagttttt
     3781 gcaatcaatt gggcgtctac tccaaaattg ccgtgacgta cgtattgatt atcaagacgc
     3841 atccattctt caaaggtaac attacttctg ccactgactt gccaaaaaca agtgagccca
     3901 ggagtaactg aaaggcgcat cttatgccaa ggcaaatatt cagcgacttc tgcaggaaca
     3961 ggaggccttg gaccaacaac agacatatcg ccttttaaaa tattaaataa ctgaggaagt
     4021 tcatctaaac taaacttgcg taagatacga ccaaaaggat aaatgcgagg gtcgtttttc
     4081 attttaaatg tggggccgcc agattcgttg tactcttgga gagccgcttt tcgctcttcg
     4141 gcatcggcgt acatgcttct gaatttatac atggtaaaaa gctttccatt tttacccacg
     4201 cgagtttgct taaaaataat aggccctttg ggatcactga ttttgaccgc tagagcaatg
     4261 attaaaagca gaggagataa aaggagaaga gctataatag cacaagaaag atctactgtt
     4321 cgtttaatga tatagcgcaa aacatttttt tggctatggt gcatgacatt nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cccaaggcca ctcccttcgg aaaggtaacc
     4501 cacgacatct gtaaacttgg tttgagaaag cattgataaa acgcaaatat tccaagtacg
     4561 taaaacaact ttatctggtt ttgcccagcc aaaaatatcg aattgacgtg caaaaatttt
     4621 tatataaaga aatggtcttt gaattcgatt tgtccttaag aattcctctt taagcctagc
     4681 ctggaataat ttggctgggt agatgggatt tccaagattt tgagaagaaa tcaaagaatc
     4741 caaatggttt tttgtcgaat cgttgctcac gtatacaaat ataaactttt tatacatcct
     4801 ttgtgagaaa aaatacagct ttattcgctc tgattttcta tcttgtgacc gtatttttag
     4861 atcggagacc tttatgcgta ttggacttat tggtactggc actgttggtg gcggcgttat
     4921 tgatattctg gaatcaaaat caaaatcatt tcaagaagat cttggattaa ctttagagct
     4981 cgcttgtatc tgtgcaagaa cagatgaaga attagcccca tttaaagcga aaggttatac
     5041 agtaactact agtgcagatc aaatgattaa ggatccttcg atcgatgttt tagttgaact
     5101 tgctggtggt tatgaaatgc cacgaaagtg gattactgcc gcattaaatg aagggaaaca
     5161 tgtcgtcact gcgaacaaag cccttcttgc aaaatacggc catgaattat ttcctttagc
     5221 agaagaaaaa ggtgctcata ttctttttga agccgctgtc ggtggtggta ttccaataat
     5281 caggagtatt caagaaggtt ttgtcggtag taagattgag catttaagct gcattatcaa
     5341 tggtacctgt aattacattc taactcgaat gtcaaaagaa ggcctttctt ttgaaagtgt
     5401 gttaaaagac gctcaggcaa aaggctatgc agaggccgac ccaacatttg atattgaagg
     5461 cgttgattct gctcataaaa cggctttgct tgcaagctta tgtagtagaa aacatgtgga
     5521 ttttgaaaaa atccatgtaa caggtgtttc aagtattaca agtgctgata ttgcttatgc
     5581 taaagaactg gattgttcca tcaaattact tggtctttat cacgcttcag gtgatcgagt
     5641 agatgctcgt gttcatcctt gtcttgtccc taatgaccac ttactttcta gtgtgaatgg
     5701 cgttttaaat gcagtctttt tagaatgtga taatttaggt cctactctac aaacaggtgc
     5761 aggtgcaggt cgtttaccta ccgcaagtgc agtggtcgct gatcttgttt ctcttgctcg
     5821 ttccgctggt aaaagagaat ctattccaat gggctggttc cagaaaaata atgaagcaga
     5881 tcttgttcct atcgaagaaa cagaggctcg ttattatttc cgttttacaa cgcaagatgc
     5941 ctgtggcgtt ttagcaaaca ttactagcat ccttgccaag aatcaaattt ctattgaatc
     6001 tattatacaa aaagatgtaa aagatcctgg aaaagtaagt atcgtagtga ttacagagaa
     6061 aacaaaagaa aaacgaatta gctctgcgat tgcaagtatt gatgctttag aaagcgtttc
     6121 agataaaagt caggtgattc gttttttaat gtagagaaaa aggaatgtta tgtttcgagc
     6181 aactactcta gaacgcacct tactagtaat atctgacttt attgcgtttt ccatttgttt
     6241 tgtaatcgca ttttgggttc agttttacag tggctggatt gcagataaat tagatgtttc
     6301 aaaacaattg ctagattatt tgccagcggg cgttctatta agttttgctt ggattatttt
     6361 cttcttttta acaggtcttt accgctcttg gcttttactt tcaagaactc agcaagctct
     6421 atatgtttta aggtctattt tatttggagt cgtcttttta attgtaggcc tttttggtag
     6481 tgagtttatt ggcaaaatca tggcctctgc tcctttgaca gaagggtatt tgtacgggtc
     6541 tcgattcatc tggatttttg cttatggctt attagccttt ttcttagttg tcttttttag
     6601 aatgttgatt tatttattct tgagacgatt attaagacaa ggttttggag ccaatcggat
     6661 gctcgtttta ggggcaacag aaatgggagc ccatgtggct gattcattta tggcctctcc
     6721 tgagcgaggg caaaaggtga tcggctttgt agatgagcgt taccaagtgt taccaaaaga
     6781 atacgctaat ttaccattac ttggtaaata ttcagatttg cctcgtattg tgcgtgaaaa
     6841 tcatgtgaga ggaattataa tttctcatga tagcacttca gttcaagaga tcatgcgaat
     6901 cttagtttgg ctttgcgatt tacctgttca tatctatatt attccgaatt tatacaatgt
     6961 tgttcatggc cattttaagg cgaatttagt tcatggtttt gaacttcaag aacttttccc
     7021 ttttaatatg cctctttggc aagttcgagt caaacgggcc atggatatta tcatcgcctt
     7081 tggtttactc attctttctt ctcccatttt tttcctcgct gccattggcg ttaaactaga
     7141 ttccaagggc cctattttct atagtcaaga gcgagttggt ttatatggcc gtcgttttat
     7201 ggttcataag tttagaacga tgaaagtgga cgcagaaaaa aatggggcgc agtgggctac
     7261 aaaaaatgat cctcgaatta ctcgtatagg gctcttttta cgtcgcgcaa gaatcgatga
     7321 attacctcaa ttactatgcg tattgaaagg cgatatgagt atggtggggc ctcgtcctga
     7381 aagagctgtt tttattgaac aacttcgtga acaagtgcct ttttatatta gtcgattaaa
     7441 aatcaaacct ggtttgactg gctgggctca agtgcgtcat cattacgata ccagtattga
     7501 agatgtgcaa aagaaattac agtatgacat gtattactat gaaaacatgt ctcttctact
     7561 agatatccag attttattcc gtacgattta tgttgtcatc acgggtaaag gagctcaata
     7621 attttatgtc cgatccaaaa cttttagaaa gttttccagt agcagatgct gtcacattaa
     7681 ttcgcttggc attaaaagaa gatgtgcgga ctggtgatat tactagtgct tggacaatcc
     7741 ctcctgaaca gaaacaaaaa gccaaattaa ttgcaaaaga gtcaggtgtt gtggcgggct
     7801 tatctattat cgatcttgtt tttcaagaat taaaagaaac tgtttctgtt acgctttata
     7861 aacaagatgg cgatgcagtt gttcctggag atttaatcgc agagctctct ggaacaacaa
     7921 gagccctttt aactggggaa cgcaccttat tgaatttcat tcagcaactc tctggcgtag
     7981 ctaccattac ttctcgttat gtggatgcat taaaagcggg ctccacccac gttttagata
     8041 cgcgtaaaac gatccctggt tttagaacac tccaaaaata tgctgttcgt gcaggaggtg
     8101 gtcttaatca caggatgggt ctttttgata tggttcttgt aaaggacaat catattgccg
     8161 cttgcggagg cgttttagaa gcacttgctg tagtcaaaaa gaataacact tcttgtgtga
     8221 gagtcgaaat agaagttgaa aatttagaag atttaaaacg cttgcttcat caaggtgttg
     8281 atgtgattat gcttgataac atggacaatg aaactatggc aaaagctgtc cagattgttc
     8341 gtgaaagtgg tgataaggtt caattagaag caagtggcaa tatgaactta gaacgcctgc
     8401 gtgaaatcgc tgcgattggg ttagattttg tctccgtagg ggctttaact catagcgtga
     8461 aggctcttga tatctcaatg aggatttaat gacaaaaggc gttattcttg tggttgatgt
     8521 tggaaactcc catactgttg ttgggatttt tagaggcgat aaagtagtgg atcattggcg
     8581 ccttacgacg cgaccaaata caacgtcaga tgaagtcctg aatcgagtga gcgggttact
     8641 tcgattttct gaaattaaac ctaatcaaat aacacatatt ggtttgagta ctgtggttcc
     8701 tgctcatgaa cgtccttgga ttaaagcgtt aacttttctt tttaataaac cagttcaagt
     8761 tgtctcttca gataattgct taaaattgcc gatcgcctat ttgaatcctt ctatggcagg
     8821 ttcagatcgt ctatgtaacg taattgcgct tcgggatcgt ggttatacgg atgccattat
     8881 tgtggatatg ggaacagcca caactttcga tgtcatgaaa gatggtggtt ttgctggtgg
     8941 tttgattatt cctggaatta gtgcaagtct agatgtttta acagaaaagg ccgcgcgatt
     9001 attgcctgtc actattgaat ggccagaaaa tctaattgca aacaatacag atgacgccat
     9061 ccgttcgggt ttgttatttg gttttaaggt cgaattagaa tcgattgtgg ctttgataaa
     9121 aaaagaattc acgaaagaaa acattcctgt ttttgcaaca gggggttggg gtaaaacgat
     9181 tgctcgtcgt acagaatgta tagatacata tgatccgtac ttaacactaa atggcatccg
     9241 tttagtggct ctatatggcg agggagctgt tgttgttgac gaagaaggag atgatgaatg
     9301 agaattctcg tgacgggtgg tgctggcttt ttagggtcac atctttgtga acgtttgcta
     9361 tcaagcggtc atgaagtgat ctgcttagac aactttttta ctgggcgtat ggataacatt
     9421 gctcatctaa gagacattac tcggtttgaa gtgattcgcc atgatgtcac ggaacctatt
     9481 ttacttgaag tcgatcttat ctttaattta gcttgcccag caagccctgt gcattaccaa
     9541 cataatccag tgaagacaat aaaaacaagt gtcatgggag ccattcacat gcttggtctt
     9601 gcaaaacgag tccatgcacg tattcttcaa gcgagtacaa gtgaagttta tggagatcca
     9661 acgcaacatc ctcagagaga atcttattgg ggaaatgtaa acccaattgg gattcgcagc
     9721 tgttatgacg aaggcaaacg cgttgcagaa acgcttttta tggattacca ccgtcaaaat
     9781 aaggtggata ttcgtatcgt tcgaatcttt aatacttatg gcccaagaat gcttgccaat
     9841 gacggtcgag tcgtcagtaa ttttatcgtt caagcgcttc aaggtcaaga tattaccatt
     9901 tatggaagtg gccaacaaac acgtagcttc tgttatgtcg acgatttaat ctcaggcttt
     9961 gtttccatga tggatcaaac acaaacagtt ggccctatta atatagggaa tccttcagaa
    10021 ttcacgatgc ttcaacttgc agaagaagtc attcgtttaa ctggaagtaa aagcaaaatt
    10081 gtctaccagc cacttcctgg agatgatcct caaaaacgtc gtcctgatat ttcaaaggca
    10141 aaagaaatat taaattggga acctgtcgtt tctttagaag aaggccttat taaaacgatt
    10201 acttattttg atcgtttaat tgaagcgggc ataaggtaac ttgcgctcaa agacccttgc
    10261 aaatagggca ttctgagacg acgtgcccac gcgctttaca aaagtttcgc ccaaagtaga
    10321 taaactgtaa atggcgtttt tcccattctt cttttggaaa tattttcttt aagtcttttt
    10381 ctgtttgttc tacagaactt ccattgctta atccgaaacg ctttgctagg cgatgaatat
    10441 gagtatctac tggaaaagca gggattttaa aagcatggct cattaccaca cttgctgttt
    10501 tgtggcccac tccagggaga gcctctaatt cttcaaatga ccttggcact tgcccttggt
    10561 gtttttcgat taggattttt gataaattga atatgtttgt gcctttcgtt ctaaaaaagc
    10621 cacaaggttt gacgatttcc atgatggttt ccacttttaa cttgatcatg gcttctgggg
    10681 ttttagcttt cttaaaaaga aacggggtaa tctgattgac tcgaacgtct gtacattgcg
    10741 cacttaaaag aaccgctatt aataaagtgt acgcatcttc atgaatcaaa gggataggtg
    10801 ggtttggaaa atattcatcc agtttagttg caataaattt gattctttcc gtacgattca
    10861 ttttagctcg ttttatatgg aaggaaggtt tatttgggat ctaattttgc gtctaaagta
    10921 aaatcttgct gcatttcaaa actaggcata tcattttctg gatgccctac ttgaacggca
    10981 ggaactccag caaaagtggt atgtggcggc acatttttta atacaacggc ccctgcacca
    11041 attttagcac ctcgaccaat gcgaatattg cctagaagtt gggcgtgagc accaagcatc
    11101 actccatccc caattttagg atgacgatct ccagtttcgt ttcctgaccc acctaaagtg
    11161 acaccatgaa aaatagagac gttattccct attttggctg tttctccaac aacaatattg
    11221 gtggcatggt caatcaaaat gccacaacca atttttgccg caggatgtaa atccattccg
    11281 aatttttgac tgataatact ttgaagcatt tttgcaggga atgttctctt ttcattccaa
    11341 agagtatggg cggtgcgata agcttgaagc ccttgaaatc ccttaaaaaa gagcacaggg
    11401 tccaaaatat tggagcaggc ggggtcacgc attttcgttg cttctaaatc ggaggccgca
    11461 ttagttaaaa tcgaaggata tttgcgaaat tgagttaaaa atagcacttc taaatctttt
    11521 ttactaacga ggtcgccccc taaaagttgt gctaaagtgg ccgcaagcat ttcaccaaaa
    11581 gaacttttgc ttaaaatttg ttggtttaaa atgtttgtag agagagcctc tttactaacg
    11641 agttctttag ctgctttaat taattctttc tcaatttcac ttattgtcac aaatgcctca
    11701 acgattgagt tttaatgtag ctttagaaag ggctcttggg gagagggggg aagtatttat
    11761 tcgctcttaa agccgctaaa gcatctaaca actcgggttc acttaataaa tccaattcag
    11821 ctaaaagctc ttcagggact cttttctgta aacggaaagc ataaggaata gcctgatgag
    11881 aagatgccca ccaaaatacg cgtaatttct taaaaaaagc aagccaaggg aaagaatcct
    11941 tagaatgaag gataaattga gctggttttt ccccgtcacg agcacttaat aataaagaac
    12001 cgtcccggaa atactgttcc aggacggcta gtcttttttt taaatgagtc gctttttgtg
    12061 gtgctatttc aagaacaatt tccatattct tgaaaataga aattattagg cgacttgcaa
    12121 ttctctttca attagagagt attgatacgt agaaatggaa gtgattttaa gaattaattc
    12181 ttttcgaaca cctgcacgaa tcaatgctct tgcgtagttt atttttgcat attcattgtt
    12241 actcataagc tgttccttta ggaatgtttc attttattta caataactta gcatttaaaa
    12301 aaaggaatta atagtgccat gtctttttta tggatacaga aaatccatga taaaatgcat
    12361 ttgattgatt atcaatgagt taagaaattt gatgttaaat attaaagtga tttaaatcac
    12421 attatttttg attggaaaag agaaaaaagg cttcgcatca tttaaaacag ggaaattttg
    12481 gcgaactttt gctaaaaaat cgtaatgaat ttctgctgaa acgacgcctt cttttgagtc
    12541 aagagaggca atgacttctc ctgttggtgc aataatcatg gagccaccat tgtactttgt
    12601 atatggatct tctccaattc gatttactcc aaggacataa cattgattct ctatggctct
    12661 tgcttttaaa agagttttcc aatgagactc tcttattgaa ggccaagaag caatcactgt
    12721 aaacagctct atattataat tcatggccgc tcgaaaaact tcgggaaaac gcaggtcaaa
    12781 gcaaataaag ggagcgacct taaaagaatc aaactgatag gttgtgattt cgtttccagc
    12841 ttcaaaatgt ttttgctctg tatgaaaagg ataaatttta tcgtattggg tgatttctac
    12901 ggtactatct gggctataaa cgctgatatg attacgaaat cgaccgttct ttttagaaat
    12961 gccactccct tgtacccaac agcccgtttc ttgagcgagc tcgtacaaaa aagatgcggt
    13021 aatcccactc tcagaatgat caaaagattc ttcaatccca tcttttgggc gccctacaaa
    13081 gccagtggca aacatttctg gcaaaacgat cagccctcct ttttcaatag gatgagattt
    13141 taataaacgg cgaacggtct caaaattctg agtcttttta caccaaagga tgtccatttg
    13201 tattaggtat acttttgcca tgattaaaat ccaggtgacc ttataattaa agggcaatac
    13261 gtggaataaa agtaacaaaa acaaagctaa aacaaataaa aaataagagt aatccttgtt
    13321 ttaggctttt atctttaaat tcactttttc aaaagagatc tttttctatt ttattaattc
    13381 ttatgtactt aatgcgatca attctttttt tattaacggc ttcaattatg ttgatagctg
    13441 ctccaacaag attggtaggg gttgttttag aagctggttc cgatcgtcca gtggctgacg
    13501 tcaatatctt atatatgtca ggcaaaaaaa tttcatccac agatgctcga ggacgctttg
    13561 atatcgaagt aacttctgaa aatacggttt tattggtaaa gaaagaaggt tatgacagtt
    13621 taactgtaaa tgtttctgag tttcctgatt ttctcgatat ggtgattctt gtctctacca
    13681 atgtacgcga tttagggcaa agtactgtga ttggtggaga aaatattgtt gagtgggaaa
    13741 atgtccgtca tgttgagctt gataaattgg aagatgctgc tggaatgcgt tttgatttga
    13801 ccgagcattt aagtcaaatg tctggtattt ctgg
//
LOCUS       DAOOBB010000033        13807 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_5725_length_13807_cov_4.042321, whole genome shotgun sequence.
ACCESSION   DAOOBB010000033 DAOOBB010000000
VERSION     DAOOBB010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 13807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13807
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5725_length_13807_cov_4.042321"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..774
                     /locus_tag="PLT31_02790"
     CDS             61..774
                     /locus_tag="PLT31_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HPW94094.1"
                     /translation="MKYPKSLVIVPTYNEKENISLILPVILSQHECIEVLVVDDNSPD
                     GTGDIVQKMSDENPRIHILRRSGKLGLGSAYVMGFKWALEQGFERIFEMDADFSHAPT
                     DLVRFLEKAETSDLVLGSRYLDRKISVVNWDMKRLILSYGANLYTRIVTRLPVSDATG
                     GFKCFRREALEALNLSDMKSDGYCFQIETTFKIWKKGLRVSEIPIVFTDRTRGASKMS
                     GGIISEAFFLVLKLRLGLA"
     gene            774..2282
                     /locus_tag="PLT31_02795"
     CDS             774..2282
                     /locus_tag="PLT31_02795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPW94095.1"
                     /translation="MPCSVIIIAYNSRDFIPACLASVREALIDIDSEIIVLDNGSPYP
                     LTTEVKEMFPEVIWLESKENLGFGRGCNLAATHATKPYLFFINPDTIVSKTAFSKTLK
                     YIQEKPKAGLVGCRILNEDGSLQWACRRAFPSPLAAIFKTIGLASLFPKSRFFASYNM
                     TYLDPDQETEVDAISGSFFCISDELYKTLGGFDESFFMYGEDLDLCFRAKKLGYKNFY
                     FPETNIIHFRGQSSKTRRFRSYLDFYNAMIIFNRKHKQFHIPLFLVSIGIFFASLVGI
                     FSRLIPQFWKMIFDIGIVFAVAGPISLDFAVLLALSIWFPLMCLGDYSSPQLDGSHYL
                     KYLFPIQIAVSLFAYFYMHEPPAFIASVFFALGGVIFWRRIWFWGLYFYRIFSKQRHR
                     SIIVGGSSDSLHRWFNQYDLIPGMEILGCISGVPDQATDENRMHLLGHVNELKSICQR
                     TGCRELLIYSNRSGYREYFNDLILQDLPLKQYLLIDGEKKGDFALVNLKYLD"
     gene            2344..2595
                     /locus_tag="PLT31_02800"
     CDS             2344..2595
                     /locus_tag="PLT31_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HPW94096.1"
                     /translation="MIDPDLLQMLCCPETKLPLQEADDLLLKELNLKISEKTLKNFSG
                     QVLTTPLEGLLVTVDGSRAYPVVENIPVLLADESIILKR"
     gene            2604..>3686
                     /locus_tag="PLT31_02805"
     CDS             2604..>3686
                     /locus_tag="PLT31_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94097.1"
                     /translation="MAATLESVKKAIGKKSSSLAFAWLADLERKAGKPDVALDTVNTG
                     LTYLRDYLPGLLVRSAIYFDLERYDDVIESCEAIVKKDPFCIAAWRRLGAAYQKKNQI
                     GKRNFCYRMVHDMDPLDSFWKEEYNFVPPEDIVPEEDSSVVEGNEISETQDVQNVQEE
                     SFEMPPELAVSTPPDLENEDPFSLLTNLLPSDEDGMQDTSLESLESSLDDVMEVISEK
                     EETLPEYFGGDDISETDVSSAFSSMFGMDDELPSEKPVSPFASFEIPTSIDDEEDASE
                     PEPEVSIPEEPKLSSLVEEEKPQSLSSAFDDIFGADELPEEKFDSDDFTSGFEKSSSA
                     EAPSFEESISDDFTSGFEKSSSAEAPS"
     assembly_gap    3687..3786
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3787..4790
                     /locus_tag="PLT31_02810"
     CDS             <3787..4790
                     /locus_tag="PLT31_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW94098.1"
                     /translation="EKSSSAEAPSFEESISDDFTSGFEKSSSVETPSFEESISDDFTS
                     GFEKSSSVEAPSIEDSGVDIAPQKEESLIDQIKESDDILPEIKSSSNKAEPQKAADEF
                     ISAVDSSLSALFGEDDLDLPQEKESAPTDVSKNSIENSFTALFGEDDDLVLPSKDAEQ
                     KEKNDGTLFHSLEEDVDSSFESLFGKEDDSFELKKDSPASSYSQPVPPLNPVEVGNEI
                     SGAFATLFSSDEDDDLPEEKPQERDGVDFLMSGDSDDEMAVALMKNPSASLDQDEVVL
                     DENLNTKTLAEIYYEQGLYSKALNIYQDLARKNPSDSEIQKRLSDIQRQVNEKYGDL"
     gene            4803..5903
                     /locus_tag="PLT31_02815"
     CDS             4803..5903
                     /locus_tag="PLT31_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HPW94099.1"
                     /translation="MADVRVEHLLREMVNHGASDLHVRVGVPPIYRINGELQKLSDVR
                     IDSVMMDSFLDDIMNRDQKARFERDKECDFAVGAREMGRFRVNVFRQRGTIAMVVRHI
                     KAKIPTFDELQLPEVIKEMALKKRGLILVTGTTGSGKSTCLAAMIDHINNEETCNVIT
                     VEDPIEYLHRDKKSIISQREIGVDTNSYENALRSALRQDPDVLLVGEIRDLETMQIAL
                     TAADTGHMVFATIHTTNATETIHRILSMYPPHQHEEVRLLLAECLVGIISLRLLPTRD
                     GNHRVPAAEILVNTGAIREYIADKTKVDMIEQAIAEGHMQYQSQTFDQALLSLYMEEK
                     ISLDTAMKAATNPDDFDLKIRGISGTSDRAWM"
     gene            6186..7466
                     /locus_tag="PLT31_02820"
     CDS             6186..7466
                     /locus_tag="PLT31_02820"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HPW94100.1"
                     /translation="MPRIDLDDQSLECPIVIGTENEKAIDISQLRSKTGYITLDDSFG
                     NTGSCKSAITYIDGDKGVLRYRGYAIEDLAENSSFIETAYLIIYGHLPSPSEYKLFSG
                     LLTSQEILHEGFRHHFEGFPNNAHPMAMLSAMINAMGCYQSDLYDDIDGVSHFDKSAA
                     QLISQVRTIAAAAYKMSIGQPMIYPYPNKRYAENFLHMMFSLPNNPYEVDPDILHALT
                     MFLILHVDHEQNCSTSTVRLVASSGANLHASCSAGVCALWGRLHGGANQAVVEMFNKI
                     RNADYSVKEFIEKVKKKEHGAKLMGFGHRVYKNYDPRAQILQKAAHKLLDKAGKNDEL
                     LEIAQELAECALHDDYFIERKLYPNVDFYSGIILQAIGIPLNMFTVMFAIGRMPGWIA
                     HWKEISDNPKQRIYRPRQIYTGETLRKYVPMDER"
     gene            complement(7537..9699)
                     /locus_tag="PLT31_02825"
     CDS             complement(7537..9699)
                     /locus_tag="PLT31_02825"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HPW94101.1"
                     /translation="MQVRILKKISLLSLLLLWLLQACSETTSSVEDDVNANLSSEATS
                     FNPCDLTDPTSGAVFCKDETGTYVYYITVNGETVWINNNSSSSSITDTNSLYISSSSL
                     DVIQSSSSIEKSCTSKEPSFTAEGISYYYNEQNLSYYYYDSLCTPIFLTAASSSSSEK
                     SSSSTIISVSSSSNIISSASSSASTNGLSPTITYSESEVIVTNNNNCIEVTNGITKIT
                     CGGDYYFSGSRTEGQILVESADLNNVYLYLQGLILSNSTDAPLYVRNAEKVLLVLADN
                     TVNTFTDGKERTVFYDGDTTKACIYAKDDLTIKGNGKLNVTGNFNNGIHTSNDLRFKD
                     APTIVVSAVNNAIKGKGSVEFGDETPWAGNIEVTSTAGDGIKSDEGEDTANGVTYNLE
                     KGFIWITGSTFKVTTAHDAIDAYNYIKIDNSPTITIKTGQGATGSTNSTYSYKGLKSD
                     SLLIINGGTIDINAQDDCIHSSGDITIAGGTMTLASADDAIHSDKTLNINDGNISVSK
                     SYEGFEAYIINLNGGTTSIVASDDGWNAAGGDGSGTATGGSTSFPSWGGGGGGFSSST
                     GTLNVKGGYHFVKAGGDGLDSNGDFNISGGTTIVVQTGGGGNGILDMGDGSGYKMTHT
                     GGVVLGVGTAQMFVTPNSGTYISSTSLGASQNTMICIANSSGQVLAAFKAPLSAAAGI
                     YMDNNISGHKFYVGGTFSGSLDANGFASTGTISGATQK"
     gene            10003..11487
                     /locus_tag="PLT31_02830"
     CDS             10003..11487
                     /locus_tag="PLT31_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94102.1"
                     /translation="MSLLLRPLFLGLLLLLFTGSFARSPVISAERSVNLKTLETSPLL
                     YEYHRLTTNDVKRLYYFPRRDSSWRNNEAEVEKSALVHFHNKHGALAASLIGGVEYKS
                     SEALADTIFPSVDGGIYLRGYIDSVEFMLDARIYNERHYNSTPKSFDREFLEHQKEEN
                     NSGFEYTSYARYRGHFAINMGFARLDFARDVMHWGPGYYNNLSLNQFAVPYNSMSLEL
                     QLGPLSVVSFYGDLLIHNNSMSMQNKTSRNLYGHRYELSLSNLVFGISELQVLYDNNR
                     PWLFVPIVPLFMEKGNYSENSNNGSLSFDVAYHFLNTGRIYSEFFLDDMESPVSLIRN
                     DNIEAKWAWMAGLQLGHDFRIWNRLLELGSIVEYARVEPYVYSHFHPSTAQLAHLGYP
                     LGNQSGPNSQTIDWTIYSRFNSTWNLSFHSQWLWKGDDYGSAVNDTTPTSNHMNLPKK
                     FIDGAKMEYSLSPSLSYDGQYVAFLLQITLFHDEKIYTRLGFKW"
     gene            11481..12797
                     /locus_tag="PLT31_02835"
     CDS             11481..12797
                     /locus_tag="PLT31_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HPW94103.1"
                     /translation="MVNDLQNTKKELLAPAGDLTRLSYALAYGADAVYAGQPAFSLRA
                     RENGFKNLDDLAEGISLCRKAGKKFYLTSNVIARNTKVESFQKALLAAIELKPDALIV
                     ADPGIIGFLRKEVPQQELHLSVQANTTNWLTAKYWAEQGIKRIILSRELRLEEVQEIH
                     KRVPEVELEVFVHGSVCMAMSGRCMLSNWVSHRDANQGACNNSCRMPYKLYANADVQE
                     DSYQMHQGSFSLQRSDKEDLPPIQLDEDSWGTYFMSSRDLCALEGLPALMEGDMIASY
                     KIEGRTRSIYYLSQVIHAYRLAIDACALGKPIPEEARHALMLTDNRGWMPGFGFKGPL
                     PQNYEATHVASNHGCVAAQIMEYNATEGRCFFKVKNPFDASDSFELLTPQGIFPCRIS
                     HIQNADGNLTERLHPGTNGSSLIQTDEKVNSDHFSYAFLIKKKTNL"
     gene            12818..13240
                     /locus_tag="PLT31_02840"
     CDS             12818..13240
                     /locus_tag="PLT31_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94104.1"
                     /translation="MQNFDDREMEIDERDCCYDLKDHVIPFFKRHIGELYDFIEKRRA
                     SSAERIETIDLIKLFIQDRKLPFDMRHYMNAQSDFIRKNIKEGCQNRQEIVSHWIKVY
                     AEKHRNRAILLQCLYLDRVSQEIIPAIEKMLQDFHQQK"
     gene            13550..>13807
                     /locus_tag="PLT31_02845"
     CDS             13550..>13807
                     /locus_tag="PLT31_02845"
                     /inference="COORDINATES: protein motif:HMM:NF023069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA N-terminal domain-containing protein"
                     /protein_id="HPW94105.1"
                     /translation="MENIFSVLDHVNVWQNLLDVLEKESDNPVDFAFLEAFQFAGIEG
                     SFVKLIAPDHFRQNWLESHYKALVETTLRKIDPALIGYKVSV"
BASE COUNT         4206 a         2242 c         2707 g         4552 t
ORIGIN      
        1 aacagacata tccagttgct gaagtacttc ctgagcttac actccctttg gagaaaccgt
       61 atgaaatatc ctaaaagtct agtcattgtt ccaacataca atgaaaaaga gaatatttct
      121 cttattttgc cagtaatttt atctcaacac gagtgtatag aagtattagt agtagatgat
      181 aattcacctg atggtactgg tgatatcgtt caaaaaatgt cggatgaaaa tcctcgtatt
      241 catatattac gtcgttctgg taaactggga ttaggttctg cttatgttat gggatttaag
      301 tgggctttag aacagggttt cgaacgaatt tttgaaatgg atgcagattt tagtcatgct
      361 cctacagatt tagttcgctt tttagaaaag gccgagacta gtgatttggt tttaggtagt
      421 cgttatttag atcgaaaaat tagcgttgtt aactgggaca tgaaacgatt gattttaagt
      481 tatggggcta atttatacac gcgtatagtg actcgattgc ctgtaagtga tgctactggt
      541 gggtttaagt gttttcgaag agaagctctt gaagctttaa atctatctga tatgaaaagt
      601 gatggctatt gctttcagat agaaacaacg tttaagattt ggaaaaaagg attacgtgtt
      661 tctgaaatac caatcgtgtt caccgataga acacgaggtg cttcaaaaat gagtggcgga
      721 attatttcag aagcattctt tttagttttg aaacttcgat taggtttggc ataatgcctt
      781 gttctgttat tattattgca tataactcaa gagattttat tcctgcgtgt ttagcttccg
      841 taagagaggc tttaattgat attgattcag aaattattgt gttagacaat ggatctccgt
      901 atccattgac gactgaagtt aaggaaatgt ttccagaagt catttggctt gagtctaagg
      961 aaaacttagg ctttggacgt ggctgtaatt tggctgcgac tcacgctacc aagccgtatt
     1021 tattttttat caatcctgac acgattgtct ctaaaacggc cttttcaaag acgctcaagt
     1081 atattcaaga aaagccaaaa gcgggtcttg tggggtgccg aatattaaat gaagatggct
     1141 ctcttcagtg ggcctgtcga agagcattcc cttcaccttt agccgctatt tttaagacta
     1201 ttggccttgc ttcattattt cccaaaagtc gtttttttgc atcgtataac atgacttatt
     1261 tagatccaga tcaagaaaca gaagtcgatg cgattagtgg atcctttttt tgtatcagtg
     1321 atgaattata taaaacttta ggaggatttg atgaatcctt ctttatgtat ggagaagacc
     1381 tagatctttg ttttcgtgca aaaaaactgg gctataaaaa cttttatttt cctgaaacca
     1441 atattattca ctttagaggg cagagtagta aaacaagacg tttcagatcg tatttagatt
     1501 tttataatgc gatgattatt tttaatcgta aacacaaaca gtttcatatt cctctattct
     1561 tagtttcaat aggtattttc tttgcttcgc tcgtaggtat tttttcaaga ttaattcctc
     1621 aattttggaa aatgattttt gatataggta ttgtttttgc ggtagctggg cctatttctc
     1681 ttgattttgc tgttttgtta gctctatcta tttggtttcc tctcatgtgt cttggagatt
     1741 attcttcgcc tcaattagat gggtcacatt atctgaaata cttgttccct attcagattg
     1801 cggtttcttt atttgcctat ttttatatgc atgaacctcc tgcgtttatc gcatcagtct
     1861 tttttgcttt aggtggcgtt attttttggc gacgtatatg gttttggggc ctttattttt
     1921 atcgcatctt ttcaaaacaa aggcatcgct cgattatcgt aggtggttcg tcagattctc
     1981 tccatcgatg gtttaatcaa tatgacttga tcccagggat ggaaatttta ggttgcatta
     2041 gtggagttcc tgatcaagcc acagatgaaa atagaatgca tttattaggg catgtcaatg
     2101 aacttaagtc aatttgtcag agaacaggtt gtcgagaact attgatttac tccaatcgtt
     2161 ctggttatag agagtatttt aatgatttga ttcttcaaga tttgccctta aaacagtatt
     2221 tattgataga tggcgagaaa aaaggcgatt ttgctctcgt aaatctaaaa tacctcgatt
     2281 aatatcgtgc gttttcttaa aatgatgtta tttttatcat aagtgtatga aaagaaaggt
     2341 tttatgattg atcctgattt acttcaaatg ttatgttgcc ctgaaactaa acttcctctt
     2401 caagaagccg atgatcttct tttaaaggaa ttaaatctaa aaataagtga gaaaacttta
     2461 aaaaattttt ctgggcaagt attaacaact cctttggaag ggctattggt aacggtggat
     2521 ggttctagag cttaccctgt agttgagaac attcctgtat tattagctga tgaatccatt
     2581 attttgaaaa gatagaggtc attgtggcag cgacattaga aagcgtaaaa aaagcaatag
     2641 gaaaaaagtc aagctctttg gcttttgctt ggcttgccga tttagaacgt aaagcaggta
     2701 aaccagatgt tgcccttgat actgtaaata caggattaac ttatttacgt gattatttgc
     2761 ctggtctatt agtccgttct gccatttatt ttgatttaga acgttatgat gacgttattg
     2821 agtcttgtga agcgatagta aaaaaagacc ctttttgtat cgctgcttgg cgtcgtttag
     2881 gagctgcata tcaaaagaag aaccaaattg gtaaaaggaa tttttgctat agaatggttc
     2941 atgatatgga tcctttagat tctttttgga aagaagaata caattttgtc cctcctgaag
     3001 atatcgtccc agaagaagat tcttctgttg ttgaaggaaa cgaaatttca gaaacacaag
     3061 atgtgcagaa tgtgcaggaa gaatcatttg aaatgcctcc agaacttgcc gtatctacac
     3121 ctccagattt agaaaacgaa gatccattct ccttattaac taatcttttg ccttctgatg
     3181 aagatggaat gcaagataca tctcttgaaa gtttggaaag ctctttagat gatgtaatgg
     3241 aagtgatttc ggaaaaagaa gaaactctac cagaatattt tggtggagat gatatttctg
     3301 aaactgatgt ctcgtctgct ttctcaagta tgtttggcat ggatgatgag ttaccttctg
     3361 aaaaacctgt ttcaccattt gcatcttttg agattccaac aagtattgat gatgaggaag
     3421 atgcttctga accagaacca gaagtatcca ttccagaaga accaaaactt tctagcttag
     3481 tggaagagga aaaacctcaa agcctttcaa gtgcttttga tgatattttt ggggctgatg
     3541 aattaccaga agaaaaattt gattcagacg acttcacttc aggttttgaa aaatcaagtt
     3601 cagctgaagc accaagtttt gaagagtcta tttcagacga cttcacttca ggttttgaaa
     3661 aatcaagttc agctgaagca ccaagtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnttga aaaatcaagt tcagctgaag caccaagttt tgaagagtct atttcagacg
     3841 atttcacttc aggttttgaa aaatcaagtt cagttgaaac accaagtttt gaagagtcta
     3901 tttcagacga cttcacttca ggttttgaaa aatcaagttc agttgaagca ccaagcattg
     3961 aagatagtgg tgtagatatt gctccacaaa aggaagaatc tttaattgat cagataaaag
     4021 aatcagatga tattcttcca gaaattaaat cttcttctaa taaagcagag cctcaaaaag
     4081 ccgctgatga atttatttct gctgtagatt caagtttatc tgctcttttt ggagaagatg
     4141 atttagatct acctcaagaa aaagaatctg ctccgaccga tgtatcaaag aatagtatag
     4201 aaaattcgtt tacggctctc tttggcgaag atgatgatct tgttttacct tcaaaagatg
     4261 cagaacaaaa agaaaaaaat gatggaacat tatttcactc tttagaagaa gatgtagata
     4321 gttctttcga atctcttttt ggaaaggaag atgattcttt tgaattaaaa aaagattctc
     4381 ctgcgtcttc ttatagtcag cctgttcctc ctttgaatcc tgtggaagtg ggaaatgaaa
     4441 tttctggagc ttttgccact ttattctctt cagacgaaga tgatgatctt cctgaagaaa
     4501 aacctcaaga gcgtgatggc gttgattttt taatgtcagg tgattctgat gatgaaatgg
     4561 ctgtggcatt aatgaaaaat ccatctgcat ctttagatca agatgaagtg gtcttggatg
     4621 agaatttgaa tacaaagact cttgctgaga tttattatga acagggtctt tattctaaag
     4681 cattgaatat ttatcaggac cttgctagaa aaaatccttc ggattcagaa attcaaaaac
     4741 gtttaagtga tattcagcgt caggtcaatg aaaaatacgg tgacctttaa aaaggatatt
     4801 ttatggcaga cgttcgtgta gagcatttat tgcgtgaaat ggttaatcat ggggcatccg
     4861 atttacatgt tcgtgtaggt gttcctccta tttatcgtat taatggtgaa cttcaaaaat
     4921 tatcggacgt tcgaatcgat tctgtgatga tggattcttt tttagatgat ataatgaatc
     4981 gagatcaaaa agctcgtttt gaacgcgata aagaatgtga ctttgctgtt ggagctcgag
     5041 aaatgggccg tttccgtgtg aacgtctttc gccagcgtgg aaccattgca atggtggttc
     5101 gtcatattaa agctaaaatc cctacatttg atgagcttca attacctgaa gtaatcaagg
     5161 aaatggcctt aaaaaagaga ggcctcattc ttgttactgg aacaacggga tctggtaaat
     5221 ctacgtgtct tgcagcgatg attgatcata tcaataatga agaaacatgt aatgtgatta
     5281 cagtcgaaga tcctattgaa tatcttcatc gcgataaaaa atccattatt tctcagcgtg
     5341 aaattggtgt tgatacaaat tcttatgaaa atgctcttcg ttctgctttg agacaagatc
     5401 cagatgtttt gcttgtcggt gaaattagag atctagaaac catgcagatt gctctaaccg
     5461 ctgcagatac gggtcatatg gtttttgcaa cgattcatac aacgaatgcg acagagacaa
     5521 tacatcgtat cctttccatg taccctcctc atcaacatga agaagtgcgt ttacttttag
     5581 ctgaatgttt agtgggtatt atttctttac gtcttttgcc tacaagagat ggaaatcatc
     5641 gtgtgcctgc agctgaaatt cttgtaaata caggtgctat tcgagagtat attgccgata
     5701 aaactaaagt ggatatgatt gaacaggcga ttgctgaagg gcatatgcaa tatcaaagtc
     5761 aaacgtttga tcaggctcta ttaagtcttt atatggaaga aaaaatttct ttagatactg
     5821 cgatgaaagc cgctacgaat ccagatgact ttgatcttaa aattagaggt atttctggta
     5881 cttctgatcg cgcctggatg taagcactct aaatacatta ttttcacgtt tttcgccaaa
     5941 aagggccttt tttcacgccc ccttgacagg atagttattt ttaaaatggt tggtgcttct
     6001 attcattgat ttttaatttg aatacagagc gaaaacgaat gatgcattgt tatcagctgt
     6061 aaaaaaagaa cttttaaagt acttgtagtt taaaaaagaa tttttattat cctcaatcat
     6121 taaacaggta ataattgatt cgtattatta gatagctact gctatctcgg ttaggagaac
     6181 ttaatatgcc aagaatagac cttgatgatc aatctttgga atgtccaata gtaataggga
     6241 ctgaaaatga aaaagcaata gatatttctc aattaagatc aaaaacgggc tatattactc
     6301 tcgatgattc ttttggaaac acaggtagtt gtaaatcggc tataacttat atcgacggag
     6361 ataagggcgt tttacgttac cgtggctatg cgattgaaga tcttgctgaa aatagttctt
     6421 ttattgaaac ggcttattta attatctatg gccatttgcc ttctccatct gaatataagc
     6481 tgttttctgg tttacttact agtcaagaaa ttttacacga agggtttcgt catcattttg
     6541 aagggtttcc aaataatgca catcccatgg caatgctttc ggcgatgatt aatgcgatgg
     6601 gttgttatca atctgatttg tatgatgaca tagacggtgt atcccatttt gataaaagtg
     6661 cggcccagct gatttctcaa gtgagaacga ttgctgcagc tgcttataag atgagtattg
     6721 gtcaacctat gatttatcct tatcctaata agcgttatgc agaaaacttt ttacatatga
     6781 tgtttagtct tccaaataat ccttatgaag tggatcctga tattttacat gcattaacga
     6841 tgtttttaat tcttcatgtg gatcatgaac aaaattgttc tacaagtact gttcgattag
     6901 tggcttccag tggtgccaat ttacatgcga gctgttcagc tggagtttgt gccctatggg
     6961 ggcgtttaca tgggggagcg aatcaggctg tcgttgaaat gtttaataaa attcgaaatg
     7021 ccgattattc ggtgaaagaa tttattgaaa aagtgaagaa aaaagaacat ggtgctaaat
     7081 taatgggttt tggacaccgc gtttataaga attatgatcc acgagctcaa attttacaaa
     7141 aagcagctca taaattattg gataaagctg gcaaaaatga tgaactttta gaaattgctc
     7201 aagaactcgc tgaatgtgcc ttacatgatg attattttat tgagcgtaaa ttgtatccta
     7261 atgtggattt ttattcagga attattcttc aagcgatagg aattccttta aatatgttta
     7321 cagtgatgtt tgccattggg cgtatgcctg gctggatcgc tcattggaaa gaaatttcag
     7381 ataatccaaa acagcgaatt tatcgtccaa ggcagatata tactggtgaa acccttcgaa
     7441 agtatgttcc tatggatgag cgttaatttt atcaataaaa gaaggaattc cttgcggaac
     7501 tccttctttt gtggttttgg tggaatctga aacgacttat ttttgagtgg ccccagaaat
     7561 ggttcctgtg gatgcaaatc cattggcatc taaagaacca gagaatgtcc cgccgacata
     7621 gaatttatga ccactgatgt tattatccat ataaatacca gcggctgcgc ttaaaggggc
     7681 cttaaatgcg gcaaggactt gaccgctgct atttgcaata caaatcatag tgttttgaga
     7741 ggctcctaat gaagtgctgc tgatataggt gccactattt ggggtgacaa acatttgagc
     7801 tgtacctact cctaatacaa cgccgccagt atgagtcatt ttataaccac tgccatctcc
     7861 catgtcgaga ataccgttgc cgcctccacc tgtttgtaca actatcgtag tacctccaga
     7921 aatattaaaa tctccgttcg aatcaagacc gtctccgccc gctttaacaa agtgataacc
     7981 tcccttgaca tttagagtcc ctgtagagga agaaaatcca ccacctccgc cgccccaaga
     8041 aggaaagctt gtagagcctc ctgttgctgt accagagccg tctccgccag cagcattcca
     8101 gccgtcatca cttgctacga tagaggttgt tccaccatta agattaatga tataagcttc
     8161 aaatccttca taagatttcg atacagaaat gtttccatca ttgatattta aagtcttatc
     8221 agagtgaatg gcatcatcag cagaagcaag agtcattgtt cctcctgcaa tagtaatgtc
     8281 accactgcta tgaatacagt catcttgtgc attgatatca atcgttccac cattaatgat
     8341 taaaagagaa tctgatttta gccccttata gctgtaggta ctgtttgtag agcctgtggc
     8401 tccttgacct gttttaatgg taatcgtagg agagttatca attttaatgt aattgtaggc
     8461 atcaatggcg tcatgagctg tggtaacctt aaatgtacta cctgtaatcc aaatgaagcc
     8521 tttttctaga ttataggtta caccatttgc agtgtcttct ccttcatcgc tcttaatacc
     8581 atcgccagca gtacttgtca cttcaatgtt tcctgcccat ggggtttcat ctccaaattc
     8641 tacggaacct tttcctttga tcgcattatt aactgcactt acaacaatcg ttggagcatc
     8701 cttaaatcga agatcatttg aagtgtgaat accattgttg aaattaccag ttacatttaa
     8761 ttttccattc ccttttatgg ttaaatcatc tttagcataa atacacgctt ttgttgtatc
     8821 tccatcataa aaaacagttc gttcttttcc atctgtgaat gtattaacgg tgttatctgc
     8881 taaaacaagt aaaacttttt ctgcgttacg aacataaaga ggggcatctg tactattaga
     8941 taaaatgagt ccttgtaaat agaggtaaac gttattaaga tctgctgatt cgactagaat
     9001 ttgcccttct gtacgggacc ctgaaaaata atagtctcct ccgcaagtga tttttgtaat
     9061 gccattggta acttcaatac aattattgtt attagtcaca atgacttcac tttcagagta
     9121 ggtaatggtg ggtgagagac cattcgtaga tgcacttgaa gatgcacttg aaataatatt
     9181 agaagaagag ctaacagaaa taatagttga agaagaactt ttttctgaac tacttgaact
     9241 tgctgcagtt aagaaaatag gggtacataa tgaatcataa taatagtatg acaagttttg
     9301 ttcgttataa taataagaaa tgccttctgc cgtaaaagag ggctcttttg aagtgcagct
     9361 cttttctata gaagagctgg attgaattac atctaaagaa gaagaactga tatacaatga
     9421 attcgtatcc gttatggacg aagaactgct attattgttt atccatacag tttctccatt
     9481 gactgtaatg taatagacgt aagttcctgt ttcatcttta caaaaaacag ctccgctagt
     9541 aggatctgtt agatcgcaag gattaaaaga agtggcttct gatgaaaggt ttgcgttaac
     9601 atcgtcttct acggacgatg tcgtttcgct acaggcttgt aatagccata ataaaagaag
     9661 agagagtaaa gaaatttttt ttaaaattct tacttgcata gtgaatcctt tttcttaaaa
     9721 gagttaatgc tttatacgtt tacattgata aagatatcca ttactcattt ttttaataag
     9781 aagttttatg tttaaaattt gtgaaaaaat gttttctctt tcatagaata aatgaaatgg
     9841 aacgaaagac tccaaatttt aggagcaaaa aaaagaagtg gaacaaaaag ctccgaaaaa
     9901 acaaactaaa aaaactcgaa atataaaggg atggatactt ttttgagcct tcttaaaaga
     9961 ataaagtaca agtagtcagt gtgcaattat tatctttacc ttatgagttt gcttttacgc
    10021 cctttatttt taggcctttt attattgctt tttacaggta gcttcgcccg ttctccagtg
    10081 atttctgcag aacgttcagt gaatttaaaa acattagaga ctagtcctct tctctatgaa
    10141 tatcatcgtt taacaacgaa tgatgtcaaa cgcctttatt atttccctag aagagattca
    10201 tcatggagaa ataatgaagc ggaagtggaa aaatcagcct tagttcattt tcataataaa
    10261 catggagcgc tagcggctag tttaattgga ggagtggagt acaagagtag tgaggcactt
    10321 gcagatacga tatttcctag tgttgacgga ggcatatatt taagagggta tattgactca
    10381 gtagaattta tgcttgatgc acgcatttac aatgagcgac attataatag tacaccgaaa
    10441 tcttttgacc gcgaattttt agaacatcaa aaagaagaaa ataattcagg atttgaatat
    10501 actagttatg caagatacag agggcatttt gccattaaca tgggatttgc taggcttgat
    10561 ttcgcgagag atgtcatgca ttgggggcca gggtattata acaacctgag cttaaaccag
    10621 tttgcagttc catataatag catgtcgtta gaattacaac taggccctct ttcggtagtt
    10681 agtttttatg gggatttatt aattcataat aatagtatga gtatgcaaaa taaaacatct
    10741 cgtaatctat acggtcatcg atatgaatta agtttaagta atttggtttt tggtattagt
    10801 gaactacaag tcttgtacga taataacagg ccttggctat ttgttcctat tgttcctttg
    10861 tttatggaaa aaggaaatta ttcagaaaac tcaaataatg gttctctttc ttttgatgtc
    10921 gcttatcatt ttttgaacac tgggcgtatt tatagcgagt tctttttaga tgatatggaa
    10981 agtccagtta gtttaattcg aaacgataat atcgaagcca agtgggcgtg gatggctggc
    11041 ttacagttag ggcatgattt tagaatttgg aatcgtttat tagaattagg ttctattgta
    11101 gagtatgctc gagtagagcc ttatgtctat agccattttc atccaagcac agcacaactg
    11161 gctcatttag gttatccatt aggcaatcaa agtgggccaa atagtcagac tattgattgg
    11221 actatttata gccgttttaa ttctacttgg aatttaagct ttcattctca gtggttatgg
    11281 aagggagatg attatggaag tgctgtgaat gatactactc caacaagtaa tcatatgaat
    11341 cttccaaaga aatttattga tggtgctaaa atggaatact cgttatcgcc atcactaagt
    11401 tatgatggtc agtacgtggc ttttttactt caaatcacat tatttcacga cgaaaaaatt
    11461 tatacccgtt taggatttaa atggtaaacg atttacaaaa tacaaagaaa gaattattag
    11521 ctcctgcagg agacctgact cgtctttctt atgccttagc ttatggtgct gatgctgtct
    11581 atgctgggca acctgctttt tctttacgag ctagagaaaa tggatttaag aatttagatg
    11641 atttagcgga aggaatttct ctttgccgaa aagcagggaa gaaattttat ttgacaagta
    11701 atgtcattgc tcgaaataca aaggtcgaat cctttcaaaa ggcattgctt gctgcgattg
    11761 aactaaaacc agatgcattg attgtggctg atcctggaat tattggcttt ctgcgtaaag
    11821 aagtaccaca gcaggaactc catttatctg tacaagccaa taccaccaac tggcttactg
    11881 ctaaatattg ggctgaacag ggaatcaaac gtattatttt aagccgagaa cttcgattag
    11941 aagaagttca agaaattcat aaacgcgttc cagaggtgga actagaagtt tttgttcatg
    12001 gctctgtttg tatggcaatg tctggtcgct gcatgctatc taattgggtc tctcatcgag
    12061 atgctaatca aggagcatgt aataattctt gtcgaatgcc ctataagtta tatgctaatg
    12121 ctgatgtgca agaggattca tatcaaatgc atcaagggtc gtttagtcta caacgctctg
    12181 ataaagaaga tttacctccc attcaattag atgaagactc ttggggcact tattttatga
    12241 gtagtcgaga tctttgtgcc ttagaagggc tgcctgcatt aatggaaggc gatatgattg
    12301 cgtcttataa aatagaaggt cgaactcgtt ccatttatta tttatctcaa gtgattcacg
    12361 cctatcgttt agcgatagat gcttgtgctt taggtaaacc gattcctgaa gaggcaagac
    12421 atgctttgat gttgactgac aatcgcggtt ggatgcctgg ttttggtttt aaggggcctt
    12481 tacctcaaaa ttatgaagca actcatgtgg cttccaatca tggttgtgtg gcggctcaaa
    12541 taatggaata taatgcaact gaagggcgat gcttttttaa ggtgaaaaac ccatttgacg
    12601 cgtcagattc ttttgagtta ttaacgcctc agggcatttt tccatgtcgt atctctcata
    12661 tacaaaatgc agacggaaat cttacagaaa ggttacaccc tggtacaaac ggaagttctt
    12721 tgattcaaac ggatgaaaaa gtcaattcag accacttttc ttatgctttt cttataaaga
    12781 aaaagacaaa tttgtaattc taacataaat tttagatatg caaaatttcg atgatagaga
    12841 aatggaaatt gacgaaagag attgctgtta tgatcttaaa gatcatgtga ttcctttttt
    12901 taagcgccac atcggagaac tgtatgactt tattgaaaag agacgagctt cttctgctga
    12961 acgaattgaa accatagact taattaaatt attcatccaa gaccggaagt tgccttttga
    13021 tatgcggcat tacatgaatg ctcaaagtga ttttattcgt aagaatatta aagaagggtg
    13081 tcagaatcgt caagaaatag taagtcattg gattaaggtt tatgctgaga aacatcgtaa
    13141 tagagctatc ttgttgcagt gcttatactt agatcgcgtt agtcaagaaa ttatccctgc
    13201 gatagaaaaa atgcttcaag attttcacca gcaaaaataa attctatgta aattaacctc
    13261 taattcagag gttttttttt acctatattt tatatttcca tgtcaagccc tttgaagaaa
    13321 tttttatgtg aataagatgt ttagatattt gtgacgtata gcgaatgctt cgtgtgcatt
    13381 ttgatcatga gtacttctaa attcacaaaa aaaagagaaa tagcgatttt gacaaaaaaa
    13441 agcctttttt atataaaaaa aagaaaaata gatctatttc aaaatccttt ttttacattc
    13501 ctttctgtta aacttttatt tctcgatagt gttgttttta ggattggctg tggaaaatat
    13561 atttagtgtt ttagatcatg ttaatgtatg gcaaaactta ttagatgttc ttgaaaaaga
    13621 atcggataat ccagttgatt ttgctttttt agaagctttt caatttgcag gaattgaagg
    13681 ctcatttgtt aaattaattg ctccagatca ttttaggcaa aattggctag aatcgcatta
    13741 taaagcttta gttgaaacga ctcttcgtaa aattgaccct gcgttaatag gatataaggt
    13801 tagtgta
//
LOCUS       DAOOBB010000034        13752 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_5748_length_13752_cov_4.834708, whole genome shotgun sequence.
ACCESSION   DAOOBB010000034 DAOOBB010000000
VERSION     DAOOBB010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 13752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13752
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5748_length_13752_cov_4.834708"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /locus_tag="PLT31_02850"
     CDS             complement(<1..1221)
                     /locus_tag="PLT31_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HPW94106.1"
                     /translation="MNVKTKVLCIGAGYVGGPTMTVIADKCPHIQVTVVDINQARIDA
                     WNSDSLPIYEPGLDEVVRRARGRNLFFSTDVASAIKDADIIFVSVNTPTKTFGQGAGK
                     ASDLQYWEKTARQILEVSTSNKIIVEKSTLPVRTAAAMERILNSNDKGLHFEVLSNPE
                     FLAEGTAIQDLLNPDRVLIGSHQTESGLAACKQVVDIYANWVSRDRILTTNLWSSELT
                     KLTANAFLAQRISSINSISALCERTGADVDEVAYVMGKDSRIGSKFLKASIGFGGSCF
                     KKDILNLVYLCSYYGLPEVAAYWESVVKINEWQTHRFVARMLENMFNTIAGKRIAVFG
                     FAFKANTGDTRESPANLVVRDLLEERALPVVTDPKAIPDAKRDLNDVLDKVIFEEDEY
                     QAAKKAHAVVVCTEW"
     gene            complement(1257..3356)
                     /locus_tag="PLT31_02855"
     CDS             complement(1257..3356)
                     /locus_tag="PLT31_02855"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HPW94107.1"
                     /translation="MASTPKPEETNEFLELLGDLWKYKFSVTLFLLLSGLLGAFAAYW
                     IRPVYEVNALLQIETKKNSGALMGDLGSLFSSGSPAETEIELVKSRRVMGAAVDQSGL
                     EYMSSPIGFWDRLLRKEGRIDLQEFYYPKDSIPEEYRFEPWILVAKDSVSYDLFDYDK
                     KKVLSGIVGETSVASYLGDSVRIGVIRMKAKAGQRFNVWRRSRLDAIEAFNSAFSVGE
                     RGKKTGILEFTYQDIYADRGVSVVNQVANAYLRQNVEQRSAEAQKTLSFLEKQIPEVK
                     KKLDSAEAELNDYRYRVGSVDINTETKIVLENQMRLQQQILSLQQKKEETVRLFNDSH
                     PMVKTMDRQIESIRAELSGMSFQVKKLPSKQQEILRLTSEVELDKILYTNMLNNIQQL
                     RLVAAGEMGSVRIVDYAELVTKPVKPKKKLILMVALFLGFCVSIVYVTIRKKFRSGVK
                     DSRVIERELGISVYAKIPKGTAIPVQKTMPLAVAFPDDVAVESMRTLRTSLEFMMPEQ
                     GGNVIVLSGLIPAVGKSFVSVNLASLFAGMDKKVLLIDADLRKGRLHKEFGLRKDLGL
                     SDVLVGKIAVEEGIHITSVPNLSLMVAGKVPASPAELLGSQKFEQLLVKMKSQFDVII
                     VDTPPVMLVTDAMLVCRHSDQAVMVVEYNRHSIEAIREGLNLFTKGVGERVHKSIVIN
                     KYVHSKMDGYGYKYGKY"
     gene            complement(3527..4930)
                     /gene="cysS"
                     /locus_tag="PLT31_02860"
     CDS             complement(3527..4930)
                     /gene="cysS"
                     /locus_tag="PLT31_02860"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPW94108.1"
                     /translation="MALQFYNTATRSKEPFTLPEGVPAVRIYCCGPTVYHFAHIGNLR
                     TYIFEDFLRRTLEYYGFTVNHVVNITDVGHLTSDGDDGDDKMEKGALRTGKTVWEVAD
                     YYTQAFMKDWKALNIVEPSIWCKATDHIQEQIQLVQTLEKKGYTYRTSDGIYFDSAKF
                     PRYPDFAHLDVENLQQGSRIDMGEKRSATDFALWKFSPQDQKRAMEWDSPWGIGFPGW
                     HIECSAMAMHHLGETLEIHCGGSDHIRVHHTNEIAQSECATGKPFARFWMHGEFLRMG
                     SDKMSKSAGEFLTVSLLQEKGFNPLDYRLFALTSHYRNYLNFTWEALESARESLKSLH
                     KKTDVLIGKADLIQSELALRFKNEFQEAIGDDLNMPKALGILNSMLKENLEESEKAAL
                     VLDFDRVLGLNLGSVREEYAPKQENVDVFKIDALISLRNEARKNKNWAESDRIRDELK
                     SMNITIKDGPNGTEWSL"
     gene            5183..6649
                     /gene="gltX"
                     /locus_tag="PLT31_02865"
     CDS             5183..6649
                     /gene="gltX"
                     /locus_tag="PLT31_02865"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPW94109.1"
                     /translation="MSASNRPVRVRFAPSPTGYLHVGGARTAIYNYFFAKAMKGTFYL
                     RIEDTDRKRYNEEALHDLLRDLRWLGLQWDEGPDCEGDVGPYFQSERLDIYAREIKKL
                     LDSGNAYYCFCTEERLQEVRAAQEKSGAVVTGYDRHCRNIPREEAEERIARGEKAVIR
                     FKVPETGITGFDDLIRGHIEYQNELLDDLVLIKRDQYPTYHFASVVDDHLMGTSHVLR
                     GDEWISSTPKHVLLYKAFGWEPPVFCHLPVILAAGGGKLSKRKGAASVGDFRDLGFLP
                     ETLVNFLALLGWNPGDDREVMTIQEMIDVFTLERINPKAVAFDEKKLQWMNGQHIHQA
                     DEKFLADYMVKGLEAIEIKTSSFSEDYILAVVKLLKNRAHFLTDLPIMASYFFTTPKT
                     FDEKAKTKNWGAGSRAIAQSVRKALEPLTEFNASSIEQAFHALAESSGLRLGDLVGAV
                     RLAVSGVSAGPGLWEIFELVGKQNVLSRILDAEPLMEP"
     gene            6646..7902
                     /locus_tag="PLT31_02870"
     CDS             6646..7902
                     /locus_tag="PLT31_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94110.1"
                     /translation="MRKAFLYPITSELLFCRRCQKVCSHQIFAREPYSTRGGIRPHIP
                     LLCSCKICSTYFIAFSQEFNFFCDSHKSEYVKILGHNRIIPGNWLYVKGTPRPGKVKG
                     VFHSATEEIIVISYNNGPDQKIERPFNEEEVEEYPQGYRLLPVQSGQTLIGDPIYHVP
                     RDAFGKVVGIVSDGEKEKLAVLLDNNILLFMTLPEAYQTTPNAQLHELIRFKLKDTFP
                     EVISALSYEVAQGIVFIKGNVPNIRLKKEIRQFLENIPAVRGCVDFIQVDPSVTISDN
                     LLKSNVLSVLEDLSLPIFDYDVNVENGKVTVRCYFSFEATPADLEKRLEVLEGIRELS
                     LLLELSPAETNTHKILCLNAARALKEHPKLKDTCIRVSCNGKKMILEGRVHSILQKSQ
                     AYFTAIRSTKKVSIDNKLRIVQPSEE"
     gene            7903..8880
                     /locus_tag="PLT31_02875"
     CDS             7903..8880
                     /locus_tag="PLT31_02875"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HPW94111.1"
                     /translation="MASTYEKLNRLKLRLMTSPATVEELANYMDCNTRTIFRFLKDLS
                     KENSSLRKIKNPGQPRRFFIEQTKNNVNAPLIRSLRKLHKELTDHAEIRYTKVINKAI
                     DALEGKTPETDCAPEAISLDPDFIIDHGPFSEYDISEARIERYLTAIKKGQCMKVHYM
                     QGSSGEIQKLEIRPLKLILRMGTLYLAYSTEKTKNVKPKLLVVKRIRQESLMQEFFKL
                     QEIDVQNFYKYCYGKWVGDSEKTVILKLLLLVKEPWLESQFRESHFNPPAKIKKQKNH
                     STIELNLYDSPDLRKWIIGLLPDIEIIEPVLFKEEIKKMIQTSLKALKS"
     gene            8940..9986
                     /gene="queA"
                     /locus_tag="PLT31_02880"
     CDS             8940..9986
                     /gene="queA"
                     /locus_tag="PLT31_02880"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HPW94112.1"
                     /translation="MNSYKLSDFDFDFPKELIANRTAGKGKTNILYCPKNGTARSILP
                     SAEIVDLIQPEDCLVVNNTKVIPARLFGNTAFGGHVETLLVQALIPAKSGEARWEAWV
                     KPGKAFTVGKELSIAGVSVTVEEIKEDGARVLRFSVSPAELEQVMNDQGHVPLPPYIL
                     RPDDEDDKKAYQTIFAKFAGAVAAPTASLHFSNEMLDKLKAKGVSIAEVTLHVGPGTF
                     QNISVEDFREHHMHGEFYELTEENARLINNVKEKKGRIITVGTTSTRVLETIASPDGK
                     LTAQKGITHAFIYPGYQYKIVDGLLTNFHWPKSSLILLVAALYGRENTLAAYAEAVKN
                     KMKLFSYGDGMLIL"
     gene            complement(9959..10489)
                     /gene="hpt"
                     /locus_tag="PLT31_02885"
     CDS             complement(9959..10489)
                     /gene="hpt"
                     /locus_tag="PLT31_02885"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HPW94113.1"
                     /translation="MDYELSKEPLISASQIEGRVLELGQEISSQFSVDLVLGVLTGSF
                     IFVADLVRTMPNPVQIRFIKASSYGLSQHSSGIVKINGLDEMDLKNKNVLVVDDILDS
                     GRTLSCLLEQVRLAGANIVKSAVLLDKPSRREVPLEADFVGFSIENHFVVGYGLDYAE
                     GYRSLRAIYKMSIPSP"
     gene            complement(10479..12902)
                     /locus_tag="PLT31_02890"
     CDS             complement(10479..12902)
                     /locus_tag="PLT31_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HPW94114.1"
                     /translation="MNSKNQLYFTIHGHFYQPPRENPWTGIIENQESAYPFHDWNDRI
                     AHECYSPNGASRLLSPTGRIQDIVNNYEYMSFNIGPTLMNWIRKKAPETYQHIQEADK
                     KSAERLNGHGNAIAQVYNHIIMPLASPEDRITQIRWGIRDFEFHFNRKPEAIWLAETA
                     INMDTVVDLIREGIRFTILSPTQAESFRKIGDSHWSDCSHTNIDTSRPYRIYPKDAEG
                     NTICEGYLDVFFYHPSLSSAVGFEHLLRDANVFGKRILDIFDPKKTEPQLVNIGTDGE
                     SYGHHEPFGDMCAAWLFNKYAPDHHMVPVNYGWFLEKFPPQYEVKLKNVYGEGSAWSC
                     AHGVGRWIRDCGCSTGGPGEWNQKWRTPLRDAFDHLKKLADDVFLREFPKYSSINPWE
                     ARNLYIDCLVDSKDEERSKKFLKHILKHPDNEKESAAALRLLEIQKYVMFSYTSCGWF
                     FNDLEGLEPVQNMRYALRAIELFKPFLPFGHPIKEEVLSILARAVSNEHQWNGAEIFT
                     RFAVPEIPVTMKLMAERAVVYHLKLNDIYYDKDSRIHTEKKSSRRRQTLVLSSFRDTC
                     LQEALSAKILVITDHLGRVNIVVCEGSYNDLSFVSDPNISIAELRSLYPTAYVVRLRD
                     LMSDSLKRINQLSTQKYLAKITETFSEVALQQGLTVDSLADPDHTLPDTMRKVLSLDI
                     NFRIHRGALDYLKKPSEEVFNTVKELVDESNELRTKFSFGGTGRLFHKKLMELIDLAT
                     DQLDETSVNHITGLISMADWLKLYIDKTTLENKAFLTYQHFKEKPKGPLKSLLPMFDW
                     LNFERPDGL"
     gene            complement(12920..>13752)
                     /locus_tag="PLT31_02895"
     CDS             complement(12920..>13752)
                     /locus_tag="PLT31_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HPW94115.1"
                     /translation="GIKIINTRSPEEFERRRAYAAIYTEENGRNGVTFEEARDDMYEP
                     NHFGMMMVKTGEADAFITGVYSKYSETIALAKSIIGIRKEYKHFGALHILNTTKGTFF
                     LADTLINRNPDADTLVDIAKLTHDAVRFFAHDPVMAMLSYSNFGADQEQVGGTANIVS
                     EAVAKLHRDYPEYVVDGEMQVNFALNKELRDQKYSFTKLKDKNVNTLIFPCLSSANTT
                     YKMLLEMGIGESIGPVQMGLKKPIHFTDVESSVRDIFNLTVAAVIDAIVQEKKDAEKK
                     "
BASE COUNT         4352 a         2830 c         2376 g         4194 t
ORIGIN      
        1 ccactccgta caaacaacta cggcatgagc ctttttagca gcttgatatt catcttcttc
       61 aaaaatgact ttatctaaaa catcattgag atctcttttt gcgtcaggaa tagcctttgg
      121 atcagtcacc acaggaagag ctctttcttc taagagatct cgaacgacaa gattggcagg
      181 actttctctt gtgtctcccg tatttgcctt aaaagcaaaa ccaaagacag cgattcgttt
      241 accagcaatg gtattaaaca tattttcaag catacgagct acaaaacgat gtgtctgcca
      301 ctcattaatt ttaacgacac tttcccaata cgcagccact tcagggaggc cataatatga
      361 gcataagtaa actaaattca atatatcttt cttaaaacaa gaaccgccaa atccaatact
      421 tgcctttaag aacttagaac ctattcttga atctttaccc attacataag cgacttcatc
      481 aacatcggct cctgttcttt cacaaagagc actgatacta ttaatagagc tgattcgctg
      541 agctaaaaag gcatttgccg ttaatttagt gagttcagaa ctccataaat tggtcgttag
      601 aatacgatct cttgaaaccc aattagcata aatatctacg acttgtttac aagcagcaag
      661 cccagactct gtttgatggc ttccaattag aacacgatct ggatttagta aatcttgaat
      721 tgctgtccct tcagctaaaa actctgggtt agaaagaact tcaaaatgaa gccctttgtc
      781 attagagttt aatattcttt ccatagcggc agcagtacgc acagggagtg ttgatttttc
      841 aacaataatt ttattggatg tactgacctc aagaatctga cgagccgttt tttcccagta
      901 ctgtaaatcg ctagccttac cagctccctg cccgaaagtt tttgtaggcg tgttaacact
      961 cacaaaaata atatcagcat ccttaattgc agaagcgaca tctgtactaa agaatagatt
     1021 acgtccacga gctctcctca caacttcatc taatcccggc tcataaatgg gaagagaatc
     1081 actgttccaa gcgtcaattc ttgcttgatt aatgtccacg acagtgactt gaatatgcgg
     1141 gcatttatcg gcaattacag tcattgtagg accgccaaca taaccagcac caatacataa
     1201 cactttagtt tttacgttca ttccttaaac ctttacccaa cccgattgaa ggggaattaa
     1261 tatttgccat acttgtagcc atatccatcc attttactat gaacatactt attaataaca
     1321 atcgatttat gaacacgttc acccacaccc ttagtaaaca agttcaaacc ctctcgaatc
     1381 gcttcaattg aatgtcgatt gtattccaca accatgaccg cttgatctga atggcgacac
     1441 acaagcattg catccgtaac aagcatcact ggaggggtat caacaataat cacatcaaac
     1501 tgagacttca ttttcacaag taattgttca aatttttgag accctaatag ctcagcaggg
     1561 cttgcgggca cctttccagc taccatgaga cttaaatttg ggacactcgt tatatgaata
     1621 ccttcttcca ctgcgatttt tcccaccaac acatcagata gaccaaggtc tttccgtaat
     1681 ccaaattctt tatgtaaacg ccctttgcgt aaatcggcgt caattaacaa aactttttta
     1741 tccatgccag caaaaagcga cgctaaattc actgagacaa aactcttgcc aacagcagga
     1801 atcaagccac ttaaaacaat aacgttgccg ccttgctctg gcatcataaa ttcgagcgac
     1861 gttcttagcg tgcgcatcga ttccactgct acatcgtctg ggaaagcaac tgctagtggc
     1921 atcgtttttt gaacaggaat agcagttccc tttggaattt ttgcatacac actaattccc
     1981 aattctcttt caatcacacg agaatctttc actccactac ggaatttttt acgaatggtg
     2041 acatagacga tagatacaca aaagcctaga aagagagcga ccattaaaat caattttttc
     2101 ttgggcttca ctggtttagt caccaattcg gcgtagtcca caattcgaac agatcccatt
     2161 tccccagcag ccacaaggcg aagctgctga atattgttca acatattggt gtacaatatt
     2221 ttatctaatt caacctcact cgtcagtcta agaatttcct gctgcttgga aggtaatttt
     2281 ttgacttgaa aagacatccc cgaaagctct gcccgaatcg attcaatttg acgatccatt
     2341 gttttaacca tcggatgaga atcattaaat aagcgaaccg tctcttcttt tttttgttgc
     2401 aagcttagaa tctgttgttg taatcgcatt tgattttcta atacaatctt cgtttccgta
     2461 ttaatatcta cagaacccac tcgatagcga taatcattta actcagcttc cgcactatcc
     2521 aatttctttt ttacttctgg aatttgtttt tctaaaaaag acaatgtctt ttgagcttca
     2581 gcacttcgct gttccacatt ttgacgtaaa taggcattcg ccacttgatt caccacacta
     2641 acgcctcgat cggcataaat atcttgataa gtgaactcta aaatccctgt tttttttcct
     2701 ctttcaccaa cggaaaaagc actattaaac gcctctattg catccagtct agaacgccgc
     2761 caaacattaa acctttggcc cgctttcgcc ttcatacgaa ttacaccaat acgaacagaa
     2821 tcgcccaaat aagaagctac agaggtttct cctacaatac cagaaagcac ttttttcttg
     2881 tcgtaatcaa acaaatcata agaaacactg tctttggcca ccaagatcca tggttcgaaa
     2941 cgatattctt ctggaataga gtcttttggg taatagaatt cttgcaaatc aattcggcct
     3001 tctttacgaa gcaatcgatc ccaaaaacca atgggagaag acatatattc taagccactc
     3061 tgatccacag cagctcccat cactcgacgg ctcttcacca attcaatttc agtttcagca
     3121 ggacttccag aggaaaataa gctccctaaa tcccccatta aagcaccact attttttttt
     3181 gtttcaattt gtaaaagagc atttacttcg tatacaggac gaatccaata tgcagcaaag
     3241 gcacccaata gaccagagag gagtaggaac aaagtcacag aaaacttata cttccataga
     3301 tcacctaata attctaggaa ttcgtttgtt tcttctggtt taggtgttga ggccatattt
     3361 aaaatacctt gaaataaagc ttatattttt ggacacttaa aacttgtttt agatcaaaaa
     3421 cacatatttg catttttaat ggggataagg taaaaaaaag acccctttat gtctacttag
     3481 aaaaacataa aaaagggtta agccatttga atttactgtt ttttacctat aaactccact
     3541 ctgtgccatt tggtccatct tttatagtga tattcatcga ttttaattca tctcgaattc
     3601 gatcactttc cgcccaattc ttattctttc gagcttcgtt tcgcagagaa attaaagcgt
     3661 ctatcttaaa aacatcgaca ttttcttgtt ttggagcgta ttcttctcga acagaaccaa
     3721 gatttaagcc taaaactcga tcgaaatcta gcactaaggc ggctttttct gattcttcca
     3781 gattttcttt taacatacta ttcaaaatgc caagtgcttt tggcatattg agatcgtctc
     3841 caatagcctc ttggaattca tttttaaaac gaagagctag ttcactttga attagatcag
     3901 ccttacctat caaaacatct gtctttttat gcagactttt taatgattct cttgcacttt
     3961 caagagcttc ccaagtaaaa ttcaaataat ttcggtaatg actagttaat gcaaataaac
     4021 gataatctaa tggattaaag cctttttctt gtaatagaga aaccgttaaa aattcacctg
     4081 cacttttact cattttatct gaacccatgc gtaaaaactc accatgcatc caaaaacgag
     4141 caaaaggttt tcctgtagca cattcacttt gcgcaatttc atttgtatga tgcactctta
     4201 tatgatcaga gccgccacaa tgaatttcta gagtttctcc taaatgatgc atcgccatcg
     4261 cagaacattc aatatgccat ccaggaaaac cgatccccca aggactatcc cattccatgg
     4321 cacgtttctg atcttgtggg ctaaatttcc acaaagcaaa atccgttgca gaacgtttct
     4381 ctcccatatc aatgcgagag ccctgttgta aattttctac gtctaaatga gcaaaatcag
     4441 ggtagcgagg aaatttggca ctgtcaaaat agatgccatc cgaagtacgg taagtatacc
     4501 cttttttctc aagagtttgc actaactgaa tttgttcttg tatgtggtct gtggccttac
     4561 accaaatgga aggttctacg atattcaaag ccttccaatc tttcataaaa gcttgtgtgt
     4621 aataatcagc gacttcccag acggttttac ctgtacgtaa agcgcctttt tccattttat
     4681 catcaccgtc atcgccatca cttgttaaat gccctacatc ggtaatattc acaacatgat
     4741 ttaccgtaaa gccatagtat tctaaagtcc gcctaagaaa atcttcaaaa atataagtgc
     4801 gaagattacc tatatgcgca aaatgataga ctgtaggacc gcaacaataa atgcgtactg
     4861 caggtacgcc ttcaggtaaa gtgaacggtt ctttggagcg agtagcggta ttataaaatt
     4921 gtaaagccat aatttggtaa tttagaaaaa gctttgataa aagggaatat gaagctttta
     4981 gaactaataa aaatgaggct aggaaaggga atgatctata aaagaagcgc tgctgaggtc
     5041 ctcttcgatg ataaaggctg tttttattct tatgtgaaag cttaattctc ttctttttgc
     5101 tattttttgt cattaggggt tctttaaaaa agtcgttttt ttggattacc cataaagtat
     5161 ttctgtttct atagaggata ttatgtctgc ttccaatcgc cctgttcgtg ttcgctttgc
     5221 tccaagccct actggctacc ttcatgtagg tggggctcgt acagccattt acaattactt
     5281 ttttgcaaaa gcgatgaagg gcacttttta cttacgcatt gaagatacag accgtaaacg
     5341 ctataacgaa gaagcccttc atgatttatt acgagatctt cgatggcttg gacttcagtg
     5401 ggatgaaggt cctgattgcg aaggagatgt tggcccgtac tttcaaagcg aacgtttaga
     5461 catctatgca agagaaataa aaaaattatt ggactcaggt aatgcttact attgtttttg
     5521 cactgaagaa agattacaag aagtgcgagc tgctcaggaa aagtctggag ccgtagtgac
     5581 tggttatgat cgtcactgta gaaacatccc cagagaagaa gccgaagaac gcattgctcg
     5641 cggagaaaaa gcagtgattc gttttaaggt tccagaaacg ggaatcacag gatttgacga
     5701 tttaattcgt ggacatattg aatatcaaaa tgaacttcta gatgatttag tgttaattaa
     5761 aagagatcaa taccccactt atcattttgc aagcgtggta gacgaccatt tgatgggaac
     5821 gtctcatgta ttacgaggcg atgaatggat tagttctact ccaaagcatg ttttattata
     5881 caaagccttt ggatgggaac ctcctgtatt ttgtcattta cctgttattc ttgctgctgg
     5941 tggcggtaaa ctttcaaaac gcaaaggcgc cgcctctgtt ggtgatttcc gtgatttagg
     6001 attcttacca gaaacacttg ttaattttct cgcgcttctt ggctggaacc caggtgatga
     6061 tagagaagtc atgacgattc aagaaatgat tgatgtattc accttagaac gcatcaatcc
     6121 taaggctgtt gcttttgatg agaaaaagct acaatggatg aatggccaac atattcatca
     6181 agcggatgaa aaattcttag cagactacat ggtcaaaggt ttagaagcca ttgaaataaa
     6241 aacttcttct ttttctgaag attatattct agctgttgtt aaacttctaa aaaaccgcgc
     6301 tcatttctta acagatctac ctataatggc ttcttacttt tttacaactc ctaaaacgtt
     6361 tgatgaaaaa gcgaagacta aaaattgggg tgctggttct agagcaatcg ctcaatctgt
     6421 aagaaaagca cttgagcctc ttactgagtt taatgcctct tctatagaac aagcatttca
     6481 tgctcttgct gaatcaagtg gtcttcgctt aggagattta gtgggagctg ttcgtcttgc
     6541 agtctctgga gttagcgccg ggcctggtct ttgggaaata tttgagttag ttggaaaaca
     6601 aaacgtattg agtcgaattc tagatgcgga accattaatg gaaccatgag aaaggctttt
     6661 ctatacccca taacgagtga acttttattt tgcaggcgct gtcaaaaagt ctgttctcat
     6721 caaatttttg ctcgtgagcc gtactccact cggggtggga ttcgccctca tattcctttg
     6781 ctctgttcct gtaagatttg ttccacttac tttattgctt tttcacaaga attcaatttt
     6841 ttctgcgatt ctcataaatc agaatatgtg aaaatattag gccataatcg aattattcct
     6901 ggaaattggc tttatgtcaa aggaactcct agaccaggta aagttaaagg tgttttccat
     6961 tctgccacgg aagaaattat tgtcatcagt tacaataatg gtcctgatca aaaaatagaa
     7021 cgtcctttta atgaagaaga agttgaagaa tatccgcaag ggtatcgtct tcttcctgta
     7081 caaagcgggc aaacgttgat tggagatccc atctatcatg ttcctcgaga tgcgttcggg
     7141 aaagtggttg gtattgtaag cgatggtgaa aaagaaaaac tcgctgtatt attagacaat
     7201 aacatccttt tattcatgac tctcccagaa gcttatcaaa cgacacccaa tgcccagctt
     7261 catgaattaa ttcgttttaa gctaaaagat actttccctg aagttatttc tgctctttct
     7321 tatgaagtgg cgcaaggaat tgtttttatc aaaggcaatg tccctaatat tcgactaaaa
     7381 aaagaaattc gacaattttt agaaaacata cctgccgtac gtggctgtgt tgactttatt
     7441 caggtagacc cttctgttac catttctgat aacttattaa aatcaaatgt tctatctgtt
     7501 ttagaagact taagcctgcc tatttttgac tatgatgtga acgtagaaaa tggaaaagtg
     7561 actgttcgat gctatttctc atttgaagca acacctgcgg atttagaaaa aaggttagag
     7621 gtgcttgaag gaattcgtga attatctctt ctcttagaat tgtcccctgc tgaaacaaac
     7681 acccataaaa tattatgctt aaatgcagct agggcactaa aagaacatcc taaattaaaa
     7741 gatacctgta ttcgtgtttc atgcaatggg aaaaaaatga ttttagaagg gcgcgtccat
     7801 tctattttac aaaaaagtca agcctatttt actgccatac gttctacaaa aaaagtttcc
     7861 attgacaaca aactacgtat tgttcaacca tcagaggaat aaatggcttc cacatacgaa
     7921 aaacttaatc gattaaaatt gcgattaatg acttcccctg ctacggttga agaattggcg
     7981 aattatatgg attgcaatac acgaactatt tttcgttttt taaaagactt aagcaaagaa
     8041 aacagcagtc tacgaaaaat taagaatcct ggacagccta gacgattctt catagaacaa
     8101 actaaaaaca atgtcaatgc tccattaatt cgttctctaa gaaaacttca caaagaactt
     8161 acagatcatg ctgaaattcg ctatacaaaa gtaatcaata aagcgataga tgctctagaa
     8221 gggaaaacac ctgaaacaga ttgtgccccc gaagctattt ccttagaccc tgatttcatt
     8281 attgatcacg gccctttttc tgaatacgat atttctgaag cacgaataga acgatacctg
     8341 acggcaataa aaaaaggcca atgcatgaag gtgcattata tgcaaggttc tagcggtgaa
     8401 atccagaaat tagaaattag accattaaaa ttaatccttc gaatggggac tctttacctc
     8461 gcttattcta cagagaaaac aaagaatgta aagccaaaac ttcttgtggt gaagaggatt
     8521 cgtcaagaat ctcttatgca agaatttttc aaactccaag aaatagatgt ccaaaatttt
     8581 tataaatact gttatggtaa atgggtagga gatagtgaaa aaacagttat tcttaaactg
     8641 cttctacttg ttaaagaacc ttggctagaa agccaattca gagagtctca ttttaatcca
     8701 cccgctaaaa taaaaaaaca aaaaaatcat tctactatcg aattgaattt atacgattct
     8761 cctgatttaa gaaaatggat tattgggctt ttgcctgata ttgaaatcat tgaacctgtt
     8821 ctctttaaag aagaaataaa aaaaatgatt caaacctcat taaaagcact taagtcataa
     8881 ttttaattta cttcagtaaa ccttcttttc gccactatat tttttaaagg ctctcatcca
     8941 tgaattctta taagttatct gatttcgatt ttgattttcc aaaagaatta atagctaatc
     9001 gaactgcagg aaaagggaaa acaaatattc tctactgtcc taaaaatggg accgctcgat
     9061 ccattcttcc atcagccgaa atagtcgatt taattcaacc agaagattgt cttgtcgtca
     9121 ataacacaaa agtcattcca gcaaggcttt tcggaaacac cgcttttgga ggccatgtag
     9181 agactcttct agtacaagct ttgattcctg cgaaatctgg tgaagcacgt tgggaagctt
     9241 gggttaaacc aggaaaagcc tttaccgttg gaaaagaact ttcaattgct ggcgtttccg
     9301 tgactgtcga agaaataaaa gaagatggtg ctagagtcct tcgttttagt gtttcaccag
     9361 cggaacttga gcaggtgatg aatgatcaag gacacgttcc attaccacct tatatattaa
     9421 ggcctgatga cgaagatgat aaaaaagctt accaaacgat ttttgcaaag ttcgcaggtg
     9481 cagtcgcagc ccctacagcg agccttcact ttagtaatga aatgcttgac aagttaaaag
     9541 ccaaaggcgt ttccattgca gaagtaactt tacacgttgg gccaggtact tttcaaaata
     9601 tttctgtcga agattttcga gagcaccata tgcatggtga attttatgaa ctcactgaag
     9661 aaaatgcacg cctcattaat aacgtaaaag agaaaaaagg gcgaatcatc actgttggaa
     9721 ctacaagcac aagagtactt gaaacgatcg cttctcctga tggaaagctc acggcacaaa
     9781 aagggattac tcacgcattt atttatccag ggtatcagta taaaatcgtt gatggattac
     9841 tcactaattt ccactggcca aagagttctt taattttact tgttgccgct ttgtatggaa
     9901 gagaaaatac tctggcagct tatgcagaag cggttaaaaa caaaatgaaa ctttttagtt
     9961 atggagatgg aatgctcatc ttataaatcg ctcgcaatga gcgatatcct tcagcataat
    10021 ctaatccgta gccaaccaca aaatgatttt cgatcgaaaa gcctacaaag tcagcttcta
    10081 aagggacctc tcgacgagaa ggtttatcta aaagaacagc actttttaca atattcgctc
    10141 ccgctaaacg gacttgttct aaaaggcaag ataacgttct ccctgaatcc aaaatatcat
    10201 ccacaacaag aacgttctta ttttttaagt ccatttcatc taaaccattt attttgacta
    10261 ttcctgaact atgttgagaa agcccgtaac ttgaggcttt aataaaacga atctgaactg
    10321 gatttggcat agtgcgaact agatcggcta caaaaataaa agaaccagta agaaccccta
    10381 aaactaagtc caccgaaaat tgagaagaaa tttcttgccc taattctaga acacgcccct
    10441 caatttgaga ggcggatatt aaaggttctt tagaaagttc atagtccatc tggtcgctca
    10501 aaattaagcc aatcaaacat tggtaataat gattttaatg gccctttagg tttttcttta
    10561 aaatgctgat atgttaaaaa cgccttattt tctaaagtgg ttttatcaat atataacttc
    10621 aaccaatcag ccatagaaat gagacctgta atgtgattga ctgaggtttc atccaattga
    10681 tcagtagcta aatcgattaa ttccattaat tttttatgga ataagcgacc tgttccacca
    10741 aaagaaaatt tcgtacgtaa ttcattagat tcatccacta attcttttac cgtattaaac
    10801 acttcttcgc ttggtttctt taagtaatct aatgcaccac gatgtattct aaagttgata
    10861 tcaagactta aaacctttcg catggtatct ggtaaagtat gatctggatc agcaaggcta
    10921 tcaacagtca agccctgttg taaggctact tcagaaaatg tttctgtaat ctttgctaaa
    10981 tatttttggg tagataactg attaattctt ttaagagaat cactcatcaa atcacgtaac
    11041 cgaaccacat acgctgttgg gtatagagac ctcaattcag cgatggaaat attagggtct
    11101 gagacaaagc ttaaatcatt atagctgcct tcacaaacca cgatattaac acgcccgaga
    11161 tgatcagtaa tcactaaaat tttagcagac aaagcttctt ggaggcaagt gtcacgaaat
    11221 gaagataata ctaatgtctg gcgacgtcta gaagattttt tctctgtatg aatacgagaa
    11281 tctttatcgt aataaatatc atttaatttt aaatgataaa caacagcacg ttcagccatc
    11341 aatttcatcg tgactggaat ttctggaaca gcaaagcgcg taaatatttc agctccattc
    11401 cattgatgtt cattagaaac ggcgcgagct aaaatactta aaacttcttc tttgattgga
    11461 tgaccaaaag gtaagaaagg cttaaagagt tcaatcgctc taagagcata acgcatattt
    11521 tgaacaggct caagaccttc tagatcatta aagaaccaac cacaacttgt ataactaaac
    11581 atgacatatt tttgaatttc aagcagacgt aaagccgctg ccgattcttt ttcattgtca
    11641 ggatgtttta aaatatgttt taagaacttc ttagatcgtt cctcatcttt agaatccact
    11701 aaacaatcaa tataaagatt gcgcgcctcc cacggattta tagaagaata ctttgggaat
    11761 tctcgaagaa aaacatcatc agctaatttt ttcaaatgat caaaagcatc gcgtaaaggg
    11821 gtacgccatt tctgattcca ttctccaggg ccacctgtag aacagccaca atcacggatc
    11881 caacggccta caccatgagc acaactccat gcagagcctt cgccatacac gttttttagt
    11941 ttcacttcat attgaggagg aaatttttcc aaaaaccaac cgtagtttac tggaaccata
    12001 tgatgatctg gggcatactt attaaaaagc caagcagcac acatatctcc aaatggttca
    12061 tgatggccat aagactctcc atcagtacca atattcacca gttgaggctc tgtttttttg
    12121 ggatcaaaaa tatccaaaat acgcttacca aagacattgg catctcgtaa aagatgttca
    12181 aaacccactg cactagaaag agagggatga taaaaaaaga catctaaata cccttcgcaa
    12241 atggtattgc cttcagcatc tttaggataa attcgataag gacgactcgt atcaatattg
    12301 gtatgagaac aatcagacca atgcgagtcc cctatttttc taaatgactc cgcttgagtt
    12361 ggagaaagaa tggtaaaacg aattccttca cgaatcaagt ccaccaccgt atccatatta
    12421 atcgctgttt ctgctaacca tattgcttct ggtttacgat taaaatggaa ttcaaaatca
    12481 cgaatacccc aacgaatctg agttattcga tcttctggtg aggcaagagg catgatgatg
    12541 tgattataga cttgagcaat cgcattgcca tgcccattca agcgttcagc acttttttta
    12601 tccgcttctt gaatatgctg gtaagtttca ggagcctttt ttcgaatcca attcattaat
    12661 gtgggtccga tattaaaact catgtattca taattattaa cgatatcttg aatgcgtccc
    12721 gttggtgaaa gtaatctact agccccatta ggactataac attcatgggc tattcgatca
    12781 ttccaatcat gaaaagggta tgcactttct tgattttcta taataccagt ccaaggattc
    12841 tctcgtggag gctgataaaa gtgtccatgt atcgtaaaat aaagctgatt ttttgaattc
    12901 atagtcgtaa tataaattcc tatttctttt cggcgtcttt tttctcttgc actatggcat
    12961 caatcacagc agccacagtc aaattaaaaa tatcccgtac tgaactttct acatctgtaa
    13021 aatgaatcgg tttttttagc cccatctgta caggccctat cgactcgcct atacccattt
    13081 ccaaaagcat tttataagtt gtatttgcag aacttaggca agggaaaatc agagtattta
    13141 cgtttttatc tttcaacttt gtaaaactat acttctgatc tcttaactct ttattcagag
    13201 caaaattcac ttgcatttca ccatccacaa catactctgg ataatctctg tgtaacttag
    13261 ccactgcttc acttacaata tttgcggtac cacccacttg ttcttgatcg gcaccaaaat
    13321 ttgaatatga aagcatagcc atcacaggat catgggcaaa aaagcgaacc gcatcatgag
    13381 ttaatttcgc gatatccact agcgtatcgg catctggatt acggttaatc aaagtatctg
    13441 caagaaaaaa agtacccttt gtcgtattta aaatatgaag agcaccaaaa tgcttatact
    13501 cttttcgtat tccaataata gactttgcta gagcaatagt ttcggagtac ttggaataga
    13561 ctcccgtaat aaaagcatcc gcttctcccg tcttcaccat catcattcca aaatgatttg
    13621 gctcatacat atcgtctcgg gcttcttcaa atgttacgcc atttcgaccg ttttcctcag
    13681 tataaatggc agcataagct ctacgtcttt cgaattcttc agggcttctt gtattaataa
    13741 tttttattcc tg
//
LOCUS       DAOOBB010000035        13581 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_5840_length_13581_cov_6.257134, whole genome shotgun sequence.
ACCESSION   DAOOBB010000035 DAOOBB010000000
VERSION     DAOOBB010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 13581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13581
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5840_length_13581_cov_6.257134"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            301..1650
                     /gene="mnmE"
                     /locus_tag="PLT31_02900"
     CDS             301..1650
                     /gene="mnmE"
                     /locus_tag="PLT31_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HPW94116.1"
                     /translation="MNQTIVAPMTPHGVSAIAALRVSGPAVRQVILSLFKNDKPIPRH
                     SYLGTAIDPVTQKSIDSLLYIFFESPNSYTGEDTLELFPHGNPLIVRNLMKAITSLPL
                     VRIAEPGEFTERAFLNGKLDLVQAEAVADVIHSTSISAIDNARRLLRGKFSTRVHELT
                     EIIKDLSIRLELDVDFAEEEVDPDTSSWRPKLENLHQQISRLINDFQSAETANHLPLV
                     VIYGAPNAGKSSLVNALLEEERVLVSSIPGTTRDFVEVRLLLSGGEIRLVDTAGISDQ
                     ATDLLDSLSMEKSRKIIKEADLKICLIDGTNTATLQTSLDEAAQKNALILISKADLFD
                     LEARKKDFPKDAIFISTKEDVSVHLSNLKSILNEKLFPPRQIQESFWVTSERQKKCLI
                     QAKEGIEKTLDLIEHNPAVELIAFEMQLVRRELLSITGEISSEDILQSIFSGFCIGK"
     gene            1640..2485
                     /locus_tag="PLT31_02905"
     CDS             1640..2485
                     /locus_tag="PLT31_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HPW94117.1"
                     /translation="MANSYIGALLAIIAFGTYMAPLKKWPLFSSWTFLPVLSWGLFIS
                     ANIVGLITNTINWNYIGLLCGFIWVGGGALCFWAVQKEEDLAGTGVRSMGVSILTSFL
                     IGIFLFKESTHLYLSIPAIIAILFGLSLLAPKGMKFLRNWRSLGAGAIFGSYLIPYQL
                     SKMDYMDFVYPFSAGIFITSHLLIFFLFKKKAIQITPIKGAVFASIGAGVLWMIGTHG
                     CFWALNGLGYAIGYPLTQLNLLVNLLWGVVIFKEYPTTNERLRIGFAAAIILTGALFL
                     TLARA"
     gene            complement(2509..3423)
                     /locus_tag="PLT31_02910"
     CDS             complement(2509..3423)
                     /locus_tag="PLT31_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94118.1"
                     /translation="MLGIEQKKGNPNKLQGKLIVYAKISSDAELEKGMLPLRNMVRNG
                     LLVVSGEFKPNESFKDFIRREVNSNSNTEGLSELIEHLKEEGMDLPDNLDPESFKDRI
                     QEISNMEIIPVPAKVVFYETEEEILKEDADIYYIGTFSGIGQAHLCITSLPIFYQAMY
                     REQVNSWERQYINDLLGQIENGDSKESENNLSNVDVFSEHESLDTFMGNLWELLGIRI
                     IPFLLAQETDEDYENNIRKFYSFMSSYPEIEDVVQMDHSIRLLRQNKGDPKARERLEL
                     LGQKISATYQEDFEKASSFKKRLEMLEN"
     gene            3595..4350
                     /locus_tag="PLT31_02915"
     CDS             3595..4350
                     /locus_tag="PLT31_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94119.1"
                     /translation="MDSTWAHILSICEQLSTTTYENLTKIIRFSKAQSADQARNFDDS
                     FQNDLDFWMGGGTCFSMTWHLYQQLQKLNLSPRLLMGHKRKEQNIHCALLLPFENKTY
                     LFDPGYLIFDPLEIPEPAPIGTGISFFPLIPNTVQLQNNGSILSLFTGSFQSPLKLRF
                     EFILSGVDVLEFQKHWTESFHREMMTYPVLNRLDKKNGIQYYYQKGNLVTRKKEGSFI
                     QKIEKENQISTLSHLFQLAPEIVEEALKIIEKR"
     gene            4604..5089
                     /locus_tag="PLT31_02920"
     CDS             4604..5089
                     /locus_tag="PLT31_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94120.1"
                     /translation="MNKLLLSFIVLFSFALIACGPSRTQIQEMSSACDALIEVRYVQN
                     DSIAIFVGNTLFVNTQQIVRDEIFPFLVSKRNPNDIDIPTATTVLNNKEEFINYLKSS
                     VSPLTSFGIVIGENVSNEIGFSEEEAIQKLTGLLSSIEGGTAVLFHEKGGQLIKAKKL
                     Y"
     gene            complement(5086..5742)
                     /locus_tag="PLT31_02925"
     CDS             complement(5086..5742)
                     /locus_tag="PLT31_02925"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPW94121.1"
                     /translation="MNFIRIVDLISDVKKQDKVVFLLRHAERRHITKEDAQFGANVGL
                     TEEGKAQAFKLGALFPTIGDASYYSSPVGRCKETAAFIAKGRGDTDSNSPEKIEVLDA
                     LGNFFVKDYDSYAEALKNNFYQNVVDYIKQGEHPAFLPLKEASEEFKEILLEKSFDQW
                     NFFLSHDAWIVPCLSHFLNIEFSLNRWMNFLSGLAIVISPSGNKLYPVTGLQDGYINY
                     "
     gene            complement(5739..6275)
                     /locus_tag="PLT31_02930"
     CDS             complement(5739..6275)
                     /locus_tag="PLT31_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPW94122.1"
                     /translation="MLQKIQLLDQRITLFMKEHRFSPKVNSWLRFYVRLGDGYIWAII
                     ALLLIYLLGWRSFLTVLWQSLDSLAVSLALYWLVKLSVKRVRPFSFFDHIDAEVPPLD
                     KYSFPSGHTMNNLAVACTVFFLVPQVGWVMLLLPVTWGLLRIYFGVHWASDIVAGIFL
                     GGISFLIGHFLWNLFIGL"
     gene            6463..7632
                     /locus_tag="PLT31_02935"
     CDS             6463..7632
                     /locus_tag="PLT31_02935"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HPW94123.1"
                     /translation="MKILVLNCGSSSVKFAVIDTKNKMAISSGLVENIGVNGHIKAKG
                     PINPIDEFLDVPTHAEAILAIQQYLKKQDLINVIEAIGHRVVHGGKYVKSEPVTQEVI
                     DYIRSITLFAPLHEPAHAIGMEEAKKYFPSLPQVAVFDTAFHQTMPPKAFLYGIPYEI
                     YEKQNVRRYGFHGTSHRYVSGEAIRLLNKKPEDTYLITAHLGNGSSAAAISQGRCVDT
                     TMGFTPLDGLVMGTRSGSIDPAILFFLNKTLGYDIDRLDKLLNKESGLLGLSGLSNDM
                     RTITQAASEGHERAKIALEVFCYKLAREIGGLAMALPRIDALIFTGGIGENSVIVREL
                     VLKNLSILGYVVDEEKNANHGKQSSGVITKDGSAKAMIIATNEELLIALDTEAIV"
     gene            7844..9205
                     /locus_tag="PLT31_02940"
     CDS             7844..9205
                     /locus_tag="PLT31_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl esterase"
                     /protein_id="HPW94124.1"
                     /translation="MGVRLFPLVFTSIAFTASALFAFSISGSVSSSKGGPIEGAAVSV
                     LNIPSISSVQTDINGNFSLEHTTSIQPNQSGLKSWKVKWNRQFLEIENIHSGSFQLSL
                     IDALGKVVWEDRISGSNGHYTLDVSTISAYKVSFLRIKDSNKESTLLLSKEAVSTKSE
                     VLLPAILFKKTGYQDTTYTMSSENETNVSIVMRDTTTEVLACPSSVLAPGDQNKTITI
                     DGKSRQYILHVPNAYKGDSPVPLVVDFHPIGGSASGESGSSPYKAKTDPEGVITVYPN
                     GLSGPMGNAWDVGPCCSDANDTLFARKIVEEVKTLACINPKRVYATGFSMGGGMSHYT
                     ACHLADIFAAVAPAAFDLLQENQDACKPVRPISVISFRSTGDPIVPYNGGLSSVVQGM
                     PITFLGAEGTHKKWAEINQCTGNPTIEKNGCSTYSNCANGVKVTLCTKQGGNHDYGNA
                     EVG"
     gene            complement(9287..12373)
                     /locus_tag="PLT31_02945"
     CDS             complement(9287..12373)
                     /locus_tag="PLT31_02945"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPW94125.1"
                     /translation="MRILHVRFENINSLAGKWEVDFTNKEFVENGLFSIVGPTGSGKS
                     SILDAITLGLYGQTPRQKNVRSNNEIMTYGTANCSAIVTFEVNGVIYRSYFEQKRARG
                     KIDGQLQGQKLSLYKIKEDETEESLTDKNTETTKKIEELIGLDYEQFTKAVMLPQGAF
                     SRFLTSDNDDRAKILEQLSGSGKYREYSKKVFEREKKEREKLNSIEEKIKSLFLLTKE
                     EEENYKAELQEKEAHQKELEKQILQLQETISWLKKIDELKKASANIASDIELLKKKEE
                     ELNPKKVLCEKARRALEVEVFYKAYQNTIEEIKRKEIARENHLSKIESIKKEIELIVE
                     KNTTLENDIEEAKKIAASKISVWDQVNELDIQIKAKKKEIEEIRKERDHITEQYQEEV
                     KKLNQLKQEWDKELSHFEIAKKYIDENQNDEFLSSDIEKLKGLFNQIEDFFKAIQEKK
                     QKLEKQKKAFTDLKQDDEKIKSSLENHRMERESFLEQELDSLSAVLREQLQENTPCPV
                     CGSLEHPFHEKEISEVKNSALENIERLKKLQEQVQNEQNELIKLQEKLKYTDKDIQDT
                     EGDLASFDEKYLSKKVEIEESLKKYSLAFNSMDEIPSLIKKLESKKEKYDKCKNQMQE
                     HQEKKSDYQLKEQAIQNLLQTIENQKQKIDVQVKAFLEELLRQQERRKEIFGEKSVES
                     DRQEVDAKRKTLEEQLKQVTNQKQEKEKARSSSEALVTSLEQEIKNLHEAKKQQEEDF
                     NANLRKAEFDSPEEFLSARMDPEECRRIEKQIQDNQKELNKKNGEYDYNQKTLQAENE
                     KKLTEKSLDELNTESVEITQKNSVFNEEIIGLRLRLEKQKENEKAKSALQDSLEKQRH
                     HHRIWDNLNKLIGSAQGDKFSRFVQGITLKMLTVNANYHLKKLNPRYFLTPQETELTF
                     KLHDSDFGETRSTSNISGGETFQISLSLALGLSSMASNRVRLDTLFLDEGFGTLDPKT
                     LQSAISLLSNLRQEEGKLIGVITHVESLKEEIETQINVLPIGNGHSRIQGPGVKKW"
     gene            complement(12370..>13581)
                     /locus_tag="PLT31_02950"
     CDS             complement(12370..>13581)
                     /locus_tag="PLT31_02950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D C-terminal
                     domain-containing protein"
                     /protein_id="HPW94126.1"
                     /translation="SDWHLGNTLKDKDRTDEFESFLTWVLDALKEEQVDALLVSGDIF
                     DVANPSHRIQEIYYRFLSKIALSGCSQVVIIAGNHDSGLLLDAPKNLLSTISIYVVGN
                     VVKGDLSGEMIELKNKDGGVEMIVCAVPYLKEAALRDVCPKEVDRDIAKLVVEGTRAH
                     YHAVFDLALQRKVELGNPEIPIVAMGHLFAANVSGGKDEGIRDVVGNLGLIDVDVFPE
                     GFDYVALGHIHRAQKIGGLNHIRYSGAPLLMGFDEIGQKRIVHIVDFPKGESCVVEKL
                     EVPSFQEFVRISGDLFTIQKELSLLREKKSNAFVEIIYNGEELINNLSYRLKDDLEEV
                     TFTVVRLMNQKEYLSGVLLDEKIEDLSEMREEEVFLKLLEVNHISEEQSQELLAAFRE
                     IIALEEDEGIV"
BASE COUNT         4071 a         2755 c         2122 g         4633 t
ORIGIN      
        1 aacttagcgg ttcaccgctg tagttgaacc atgcagagct gtgagaatga tacccctgcg
       61 gtacaaacga gctctaaagt aaaatccatt ttcgtctagt aattcactat tatgatttag
      121 ttgaagggca ttattttttt attcgagcct tcgctatttg ctttccaaac aaataacgtt
      181 tgtctcggca tgaaaaaact gggttggttt agatgggctt ttctttgttt tcttcaaaca
      241 tgcattattc gtgagagtgc tcgcttttga tttgaagtag tgatatacta tatttacaaa
      301 atgaatcaaa ctattgtcgc tccaatgacg ccccatggcg tttctgcaat agcagctctt
      361 cgagttagcg gacccgccgt tcgacaagtg atcttatctt tatttaaaaa tgataaaccg
      421 attccaaggc attcttactt aggaacagcc attgacccag tgactcaaaa aagcatcgat
      481 tctttacttt atattttttt tgaatctcca aattcatata ctggcgaaga cactcttgaa
      541 ctctttcctc atgggaatcc tttaattgtt cgtaatttaa tgaaggccat cacctctcta
      601 ccccttgttc gcattgcaga gccaggtgaa tttacagagc gcgccttctt aaatggaaag
      661 ctcgatttag tccaagccga ggccgttgct gacgtaatac actctacttc tatttcagcc
      721 atagataatg cacgcaggtt attacgcgga aaattttcaa cgcgcgtcca tgaactcact
      781 gaaatcatca aggatctttc cattcgatta gaacttgatg tcgattttgc tgaagaagaa
      841 gttgatccag acacttcttc ttggcgccct aaactagaaa atcttcatca acaaatttca
      901 cgtttaatta atgattttca aagtgcagaa actgcaaatc atttgcctct agtcgtcatt
      961 tatggagccc caaatgcggg caaatcaagc cttgtgaatg cccttcttga agaagaacgc
     1021 gtactagtaa gctctattcc tggcactaca agagattttg tagaagtacg ccttttgctt
     1081 tctggtggtg agattcgtct cgtagatacc gcaggtattt ctgatcaagc gactgatctt
     1141 ttagattccc tttctatgga aaaaagccgg aagattatta aagaagccga tcttaaaatt
     1201 tgcctcattg atggtactaa cacagcaact ctccaaacgt ccttagatga ggccgctcaa
     1261 aaaaacgctt tgattcttat ttcaaaagcg gatctttttg atttagaagc acggaaaaaa
     1321 gactttccaa aagatgctat ttttatctct actaaagaag acgttagtgt tcatctatcc
     1381 aatttgaaat ccatcttaaa cgaaaaacta ttccctccta gacaaattca agaaagcttt
     1441 tgggtcacaa gtgaaaggca aaaaaaatgc ttgattcaag caaaagaagg gatagaaaaa
     1501 actcttgatt taatcgaaca taatcctgct gtagaactca ttgcgtttga aatgcaactc
     1561 gtacggcgtg aacttctttc tattacggga gagatttctt ccgaagacat tctccaatct
     1621 atattctcgg ggttctgcat tggcaaatag ttatataggt gctttattag ccatcattgc
     1681 ttttggaacg tatatggcgc cattaaaaaa atggcctcta ttttcttcat ggactttttt
     1741 gcctgttctt tcttggggct tatttataag cgctaatatt gtaggactaa ttacaaatac
     1801 catcaactgg aactacattg gcttactctg cggttttatt tgggttggag gtggtgctct
     1861 ttgtttttgg gcagtccaaa aagaagaaga tctcgcaggg acaggcgtaa gatccatggg
     1921 agtaagcatc ttaacttcat ttttaattgg cattttcctt ttcaaagaat cgactcatct
     1981 ttatctttct attcctgcta tcatagccat attatttgga ttatctttat tagcaccaaa
     2041 agggatgaaa tttttaagaa attggagatc tttaggagct ggtgccatat ttggttccta
     2101 cttaatccct tatcaactct caaaaatgga ttacatggat tttgtctatc cattttctgc
     2161 tgggattttt atcacttctc atcttttgat ttttttcttg ttcaaaaaga aagctattca
     2221 aatcactcct attaaaggag ctgtttttgc ttcgataggt gccggcgttt tatggatgat
     2281 tggtactcac ggttgttttt gggcactgaa tggcctagga tatgcgattg gatatccgct
     2341 cactcaatta aatttgctcg tgaacttgtt gtggggcgtt gtcattttta aggaataccc
     2401 taccactaat gaacgcctta gaattggttt tgcagccgcg attattttga ctggagcact
     2461 ctttttaact ttagctcgcg cctaagtaat ttggctttca acgagccatt aattttcaag
     2521 catttcaagg cgtttcttaa aactagaagc cttttcaaaa tcttcttgat aagtagcgct
     2581 tattttttgc cctaatagtt ccagccgttc gcgtgctttt ggatcccctt tgttctgcct
     2641 taataatcga atggaatgat ccatctgcac cacatcttcg atttcaggat aagaactcat
     2701 aaaagaataa aattttcgaa tgttattctc gtaatcctca tctgtttctt gagctaacaa
     2761 aaatggaatg atacgaatac caagtaattc ccataagtta cccataaaag tatctaggga
     2821 ttcgtgttca gaaaagacat ctacattact aagattattt tcactttcct tagaatcgcc
     2881 attttcaatc tgccctaata aatcattaat gtattgacgt tcccaagaat tcacttgttc
     2941 tcgatacatg gcttgataaa aaataggtag gctcgtaata cataaatgag cttgaccaat
     3001 acctgaaaat gtacctatgt aataaatatc agcatcttct tttaagattt cttcttctgt
     3061 ttcataaaaa acaactttgg caggaacagg aataatttcc atatttgaaa tttcctggat
     3121 tctatcctta aaagactcag gatccaaatt atcaggtaaa tccattcctt cttcttttaa
     3181 atgctctatt aattcagata aaccttctgt attcgaatta gaatttactt cacgccttat
     3241 aaaatcctta aaagactcat taggcttaaa ttctccagaa acgactagta aaccattacg
     3301 aaccatattt cttaatggaa gcattccttt ttcaagctct gcatcagacg aaatctttgc
     3361 atagacaatc aatttgcctt gcaacttatt tggattccct tttttttgtt ctatacctaa
     3421 catgactttt ctctcaacta aaaaacaaac agagcgttat atagaaatat aaaaaactat
     3481 ttaagagtaa cccaaaattt attatctgaa acgcaagatt tatgaaatca aggagatcat
     3541 atacaataat atgaacttct aggatttttc actcaacaat tctatttttc tatcatggat
     3601 tctacttggg cacatattct ttcgatttgc gagcaactat caacgacaac gtatgaaaat
     3661 ctaactaaaa taattcgatt ttctaaagcg cagtctgcag atcaagctag aaattttgat
     3721 gatagttttc aaaatgattt agatttctgg atgggtggcg gcacttgttt tagcatgact
     3781 tggcatttat accaacaact acaaaagcta aatctttctc ctcgcttact aatgggccat
     3841 aaaagaaaag agcagaatat tcactgcgcc ttattgcttc cctttgaaaa taaaacgtac
     3901 ttatttgatc ctggttattt aatttttgac cccttagaaa tcccagaacc agcacctatt
     3961 ggaacaggaa tctccttttt ccctttaatt ccaaatacgg ttcaattaca aaataacggt
     4021 tctattcttt ctttatttac tggcagtttt caatcccctt taaagcttcg tttcgaattt
     4081 attctctcag gtgttgacgt tttagaattt caaaaacatt ggacagaaag tttccaccga
     4141 gaaatgatga cttacccagt cctaaatcgt ttagataaaa aaaatgggat tcaatattat
     4201 tatcaaaaag ggaatttagt cacaagaaaa aaagaaggct cttttattca aaaaatagag
     4261 aaagaaaacc aaatttcaac actaagtcac ctttttcagc tcgcgccaga aattgtagaa
     4321 gaagccttaa aaattatcga gaaaagatag atcctattga gtcatctcta aaatcactca
     4381 cggataaaat taccttgata cttcaaagat cgtaagttgt taaatgtggc atatatcaca
     4441 aacatttgct atttatgtgc tgtattcgtc ttatcaagca aacatgcctt gaggttgctt
     4501 gatctattct attttgcccc atgcaaaacc aaattttaga ggttccttat ttttttaaaa
     4561 ctttttgacc ttgaattatt tattttattc atacggagat tttatgaaca aattacttct
     4621 ttcttttatc gttttattca gttttgctct tattgcctgt ggcccttccc gaacacagat
     4681 tcaggaaatg tcttctgcat gtgacgcatt aattgaagtg cgttatgtac agaatgattc
     4741 tattgccatt tttgttggta atacgctctt cgtgaacaca cagcaaatcg tccgtgatga
     4801 aatatttcct tttcttgtaa gcaaaagaaa tcctaacgat attgatatcc caacagcgac
     4861 caccgtcctc aataacaaag aggaatttat caactattta aaaagtagcg tttccccatt
     4921 aacctctttt ggtattgtca ttggagaaaa cgtttctaat gagattggat tcagtgaaga
     4981 agaagccatt caaaaattaa caggcttact gagttccatt gaaggtggaa cggctgtgct
     5041 atttcatgag aaaggtggac aattaattaa agctaaaaag ctttattaat aattaatata
     5101 accatcctga agcccagtca cgggatataa tttattccct gaaggggaga tgactatcgc
     5161 taatccagat aggaaattca tccaacgatt taatgaaaat tctatgttta gaaaatgaga
     5221 taagcatggt acaatccaag catcgtgcga taaaaagaaa ttccactgat caaaagattt
     5281 ctctaaaagg atctctttaa actcttccga cgcttctttt aatggtaaaa aagcagggtg
     5341 ctccccttgt tttatataat ccactacgtt ttgataaaaa ttatttttta atgcttctgc
     5401 ataagaatcg taatctttaa caaaaaaatt cccaagcgca tccaagactt caattttttc
     5461 tgggctatta gaatctgtat cccctcgccc tttagcaata aaagcggccg tttctttaca
     5521 acgacccaca ggacttgaat aatatgaagc atcacctatg gttggaaata gagctcccaa
     5581 tttaaaagct tgggctttcc cttcttcggt taaccctaca ttagccccaa attgagcatc
     5641 ctcttttgta atatgacgac gctctgcatg acgtagtaaa aaaacaacct tatcttgttt
     5701 ctttacatca gaaattaaat ctacaattcg aatgaaattc ataatcctat aaacagattc
     5761 caaagaaagt gaccaattaa aaaactaatt ccacctaaaa atatacccgc aacaatatca
     5821 gaagcccaat gcacaccaaa ataaattcga agtaatcccc aagtcacagg caataaaagc
     5881 atcacccaac ctacttgagg aactaagaaa aatacagtgc aagctaccgc caaattattc
     5941 atcgtatggc cagaagggaa actgtactta tctagtggag gtacttctgc atctatatga
     6001 tcgaaaaatg aaaaaggacg aactcgttta acagaaagtt ttacaagcca ataaagagca
     6061 agactaacgg ctaaagaatc taagctctgc cacaaaacag ttaaaaaact tctccaaccg
     6121 agtaaataga ttaatagcaa agcaattata gcccaaatat aaccatcacc taaacgcaca
     6181 taaaaacgaa gccaactatt cacttttgga gagaaccgat gttctttcat aaataatgtg
     6241 attcgttgat ctaaaagctg aattttttga agcatgctta aaatttatat aatttactct
     6301 tccgtcaaac cagaacgttt ttgtttttat atcttttcga atataaacat aggacacctc
     6361 taaaaattag catgtttagc gaatattatt atattcacca ttttttctag aaattatttt
     6421 ttagagttac ccgataatca cacttttctg tcaaaggttt ttatgaagat tcttgtttta
     6481 aattgtggta gttcttcagt caaatttgct gttatagaca caaaaaataa aatggccata
     6541 tccagcggac tcgtcgaaaa cattggagta aatggtcata taaaagcaaa aggccctatt
     6601 aacccaatcg acgaattctt agacgttcct actcatgccg aagctatctt ggctatacag
     6661 cagtatttaa aaaaacaaga cctcattaac gtgattgaag ccattggaca ccgagtggtt
     6721 catggcggta agtatgttaa atcagaacca gtgacacaag aagttattga ttacattaga
     6781 agcattactt tatttgcacc gctacacgaa ccagcccatg ccatagggat ggaagaggct
     6841 aaaaagtatt ttccaagtct tccccaagtt gctgtttttg ataccgcttt tcatcaaaca
     6901 atgccgccaa aagcttttct gtatggcatt ccttatgaaa tctatgaaaa gcaaaatgta
     6961 cgccgttatg gatttcatgg tacttctcat cgatatgttt ctggagaagc cattcgttta
     7021 ctcaataaaa aaccagaaga cacttattta attaccgctc atcttggcaa cggttcttct
     7081 gcagcagcca tttctcaagg gcgttgtgta gacaccacca tgggattcac ccctttagat
     7141 ggccttgtta tggggacacg ctctggctcg attgaccctg cgattctctt tttcctaaat
     7201 aaaacgcttg gttacgatat cgatcgttta gataaattac tgaataaaga aagtggctta
     7261 ttaggcctct ctggtcttag taacgacatg cgtaccatca cacaggccgc gagtgaaggg
     7321 cacgaaagag ccaagattgc tttagaagtt ttttgttata aattagcgag agaaattggt
     7381 ggccttgcca tggctttacc aagaatagac gctcttattt tcactggtgg cattggtgaa
     7441 aatagtgtaa tcgttcgcga actcgtttta aaaaatttaa gtattctagg ctatgtagtt
     7501 gatgaagaga aaaatgcgaa ccatggaaaa caatcttctg gcgtaatcac aaaggatggt
     7561 tcggcaaaag caatgatcat tgcgacaaat gaagaacttc ttattgcttt agacaccgaa
     7621 gccattgttt aattcaaacc agcaatcatt ttttggaacg ccagcctagc taacactttt
     7681 gaagaaacac aaaacaacag aattttctat aaaaatctgt tgtaaaactt taatatactt
     7741 tttggatgta aatcgattat ttacatcctc gcccattagg aaaaacgtat tatttgatta
     7801 agtgtaggga ccaagggaaa atccatattt caaaggagat gttatgggtg ttcgtttatt
     7861 tccactggtt tttacaagca ttgctttcac agcatctgct ttattcgctt tcagtatcag
     7921 cgggagcgta tcctctagta aaggagggcc tattgaaggc gcggccgttt ctgttctaaa
     7981 tattccctct atttcttctg tgcaaacaga tataaatggc aatttcagtt tagaacacac
     8041 cacttctatt cagccaaatc aatctggttt gaagagctgg aaggtaaaat ggaatcgtca
     8101 atttcttgaa attgaaaata ttcattctgg ctcatttcaa ttgtctttaa tagacgctct
     8161 aggaaaagta gtttgggagg atcgcatttc tggatcaaac ggtcattata cattagacgt
     8221 ttctacaatt tctgcttata aggtttcttt tttgagaatt aaagactcca acaaagaatc
     8281 cactcttctt ttatcaaaag aagctgtttc tactaaatct gaggtattat tgccagctat
     8341 tctcttcaaa aagacaggct atcaagatac cacttatacg atgtcttctg aaaacgaaac
     8401 caacgtttct attgtcatga gagataccac tactgaagtg ctagcttgcc cttcttctgt
     8461 tcttgctcca ggtgatcaaa ataagactat tactattgat ggaaaatcac gtcaatatat
     8521 tttacacgtt ccaaacgcat acaaaggcga ttccccagtt cctctagtcg ttgatttcca
     8581 tcctattggt ggttctgctt caggagaatc tggttcttcc ccttacaaag caaaaactga
     8641 tcctgaaggc gttatcaccg tttatccaaa cggcttatca ggacctatgg gcaatgcttg
     8701 ggatgtaggc ccctgttgtt cagatgccaa tgacacttta tttgcaagaa aaattgttga
     8761 agaagtaaaa acattagcct gcattaatcc aaaacgagtt tatgccactg gattttctat
     8821 gggtggaggc atgtctcatt atactgcctg tcatttagca gatattttcg ctgctgttgc
     8881 accagccgct tttgatttgc ttcaagaaaa ccaagatgcc tgcaaacctg ttcgacccat
     8941 ttctgttatc tctttccgca gcacaggtga tccaattgtc ccttataatg gaggtctttc
     9001 cagcgtagtc caaggaatgc cgattacctt cttaggtgca gaagggactc acaaaaaatg
     9061 ggcagaaatc aatcaatgta ctggaaaccc taccatagaa aaaaatggtt gctccactta
     9121 ctccaattgt gctaatggcg ttaaagtcac cctttgcaca aaacaaggtg gtaatcacga
     9181 ttatggtaat gcagaagttg gttgaccatt cttaaaggaa ttcacccttc cataataagg
     9241 gttaaaaaga atagaaatca ccacccaatt cacgggcggt gtttttttac catttcttga
     9301 ctcctggccc ttggatacgg ctatgaccat ttcctatggg tagcacatta atttgagtct
     9361 caatttcttc ttttaatgat tccacatgag taatcacgcc gattaatttc ccttcttctt
     9421 gacgcaaatt agaaagcaaa ctaatagccg attgtaatgt tttaggatct aatgtcccaa
     9481 atccttcatc taaaaataac gtatctaaac gcacgcgatt gcttgccatg gatgataaac
     9541 cgagagctaa agaaaggcta atttgaaagg tttcaccgcc tgagatattt gaagtacttc
     9601 gagtttctcc aaaatcgcta tcatgcaact taaaagtaag ttcagtttct tgaggtgtta
     9661 aaaaatacct aggattcaat tttttcaagt ggtaattcgc attaaccgta agcattttaa
     9721 gtgtaattcc ctgtacaaat ctcgagaatt tatccccttg agccgatcca atcagtttat
     9781 tcaaattgtc ccagatgcga tgatgatggc gttgcttttc taaagaatcc tgtaacgcac
     9841 tctttgcttt ttcattttct ttttgctttt caagccttaa tcttaatcca atgatttctt
     9901 cattaaacac actatttttt tgagttattt ctacgctttc tgtatttaat tcatccaaag
     9961 atttttccgt tagcttcttc tcgttttcag cttgtagcgt cttttgattg taatcatact
    10021 cgccattttt cttattcaat tctttttgat tgtcttgaat ttgtttttct attcgacggc
    10081 attcttctgg gtccattctc gcacttaaaa attcttctgg cgaatcaaat tctgcttttc
    10141 ttaaattagc attaaaatct tcttcttgtt gtttttttgc ttcatgcaag ttttttattt
    10201 cttgttctaa tgaagtgact aaagcctctg aagaagacct cgccttttct ttttcttgtt
    10261 tttggtttgt cacttgtttc aattgttctt ctagggtttt tctcttggca tctacttctt
    10321 ggcggtcact ctccacactt ttttcaccaa aaatttcttt tcgcctttct tgttgcctca
    10381 ataactcctc taaaaaagcc tttacctgta cgtcgatttt ttgtttttga ttttcaatcg
    10441 tttgtaaaag attctgaatc gcctgttctt ttaactgata atcacttttc ttctcttgat
    10501 gctcttgcat ctgattttta cacttgtcat atttttcttt tttactttcc aactttttta
    10561 taagagaagg aatttcatcc attgaattaa aagctagcga atattttttt aaggattctt
    10621 ctatttctac ttttttgctc aaatattttt catcaaaaga agcaagatcc ccttctgtat
    10681 cctgaatatc tttatcggta tatttcaatt tttcttgtaa ttttattaat tcgttctgtt
    10741 cattttgtac ttgttcttga agctttttta atcgctctat attttccaaa gcagaatttt
    10801 ttacctctga aatttctttc tcatgaaacg gatgctctaa tgaaccgcaa acagggcaag
    10861 gcgtattttc ttgaagctgc tctcgaagca cggctgatag cgaatccaac tcttgttcta
    10921 aaaaactctc cctttccatt ctatgatttt caagtgatga tttgattttt tcatcatctt
    10981 gctttaaatc agtaaatgct ttcttttgct tttctaattt ttgctttttc tcttgaatag
    11041 ccttaaagaa atcttctatt tgattaaata aaccttttaa tttttctata tccgatgata
    11101 aaaattcatc gttttgattc tcatcaatat atttcttagc gatttcaaaa tgagaaagtt
    11161 ctttatccca ttcctgtttt aattggttta atttttttac ttcttcttga tattgttccg
    11221 taatatggtc gcgttctttt cttatttctt ctatttcttt tttctttgct ttaatctgaa
    11281 tatctaattc gttgacttga tcccatacac tgattttcga agccgctatt tttttcgctt
    11341 cttctatatc attttctaac gttgtatttt tttcaactat gagttctatt tcttttttta
    11401 tactctctat ttttgataga tgattctctc ttgctatttc ttttctttta atttcttcaa
    11461 tcgtattttg atacgcctta taaaacacct ctacttctaa ggcacgcctg gctttttcac
    11521 acaatacttt tttaggattt aattcttctt ctttcttttt taataattca atatcacttg
    11581 caatgttcgc agaagctttc tttagttcat ctattttttt caaccaagaa atcgtttctt
    11641 gtaattgaag aatttgcttt tctaactctt tttgatgtgc ctctttttct tgcaattctg
    11701 ccttataatt ctcttcttct tctttcgtta gtaaaaacaa acttttaatt ttttcttcaa
    11761 tagaatttaa tttttccctt tccttttttt ctctctcaaa aacttttttt gaatactctc
    11821 gatacttgcc agatcctgat aattgctcca gtattttggc tcgatcatca ttgtctgaag
    11881 ttaaaaaacg actaaaagca ccttgtggaa gcatcacggc tttagtaaac tgttcgtaat
    11941 ctaaaccaat caattcctca attttcttgg ttgtctctgt atttttatct gttagagatt
    12001 cttcagtctc atcttctttt atcttataca aagataattt ttgcccttgt aattgaccat
    12061 ctatttttcc gcgcgctcgt ttctgttcaa aataagaacg ataaataact ccattcactt
    12121 caaatgtcac tatcgctgaa caattcgccg tgccataagt catgatttca ttattacttc
    12181 tcacattttt ttgacgaggc gtttgcccat ataatccaag cgtaatcgca tctaatatag
    12241 agcttttccc agagccagtg gggcccacaa tagagaacaa accattctct acaaattctt
    12301 tatttgtaaa atcaacttcc catttgccag caagagaatt tatgttttca aaacgtacgt
    12361 gtaaaattct catacaatac cctcatcctc ttcgagagcg ataatctctc gaaacgcagc
    12421 taataactct tgactttgtt cttctgaaat atgattgact tctagtaact ttaagaaaac
    12481 ctcttcttca cgcatttccg ataagtcttc aattttttca tctaaaagaa cacctgataa
    12541 atactctttt tgattcatta aacgaactac cgtaaaagtc acctcttcta aatcatcttt
    12601 taagcgatag cttaaattat tgatcaattc ttcgccatta taaataattt ccacaaaagc
    12661 atttgacttt ttttctctaa gtaaagaaag ctctttttga atagtaaaaa gatctccact
    12721 aatacgaaca aactcttgaa aagaagggac ctctaatttt tcaacaacac aagactctcc
    12781 tttcggaaaa tcaacaatat gaacaattcg cttttgacct atttcatcaa agcccatcaa
    12841 aagaggcgcc ccgctatagc gaatatgatt taatccccca attttttgag ctctatgaat
    12901 atgccctaaa gccacataat caaaaccttc aggaaacaca tctacatcaa ttaaccctaa
    12961 attcccgacc acatcacgaa tcccttcatc tttaccgcca ctcacattag ccgcaaacaa
    13021 atggcccatc gccactattg gaatttcagg attccctaat tctacttttc tttgtaacgc
    13081 taaatcgaac accgcatgat aatgagctct tgtgccttcg acgactaatt ttgcaatatc
    13141 tcgatccact tcttttgggc aaacatcacg taaagcggct tctttcaaat aaggaaccgc
    13201 acaaacaatc atttcaacgc cgccatcctt atttttcaat tcaatcatct cgcctgataa
    13261 atcgcctttc accacattcc cgaccacata aatcgaaatc gtagacaaaa gattcttggg
    13321 cgcatctaat aacaaaccag aatcatgatt ccctgcgatg atgaccactt gcgaacaacc
    13381 actcaaagca attttcgata aaaaacgata atagatctct tgaatcctgt ggcttggatt
    13441 cgccacatca aaaatatccc cagagaccaa cagagcatcg acctgctcct ctttcaaagc
    13501 atccaaaacc caagtcaaaa acgactcaaa ttcatctgtt ctatctttat cttttaaggt
    13561 gttccccaaa tgccaatccg a
//
LOCUS       DAOOBB010000036        13528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_5872_length_13528_cov_4.680398, whole genome shotgun sequence.
ACCESSION   DAOOBB010000036 DAOOBB010000000
VERSION     DAOOBB010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 13528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13528
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5872_length_13528_cov_4.680398"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="PLT31_02955"
     CDS             complement(<1..473)
                     /locus_tag="PLT31_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPW94127.1"
                     /translation="MENKILIVEDEEIIRLGLQDNFELENYIVETASDGEEAIAKADS
                     FKPDLVILDLMIPKKSGFEVCRVIRKKHPKCFIIMLTAKTEETSKVAGLEMGADDYVT
                     KPFSILELLARVKAFLRRSADLKTAIESNTTVVPDVIQFADIRLDFKKYEAFKKD"
     gene            complement(475..2520)
                     /locus_tag="PLT31_02960"
     CDS             complement(475..2520)
                     /locus_tag="PLT31_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPW94128.1"
                     /translation="MRFSKSNPILVAFFFGGIILPTAILSVLSFRNIQNETYLAKKNF
                     EENLKSFKKELENVVQKEQGKILQETKAASQFLYEQPQNLLEFGNASSFKNVKGLSAI
                     FLFNKETIVYPNIASHFYYESSKWPQTNYSSIEQQLFFEEINKTNEEKVKLARSLRLM
                     QNPFQSRSEQVQNLLGLLRNAYQNKKYDEALHIISLLQQRPNLQSYLHYQLRESLQLL
                     HFEILVEQNKHEEAQNLCLLELNRFLQQPHINDISATRYFFETTFNQILSFEKLTQET
                     REAFWNLRENFNRQLTHTEILYEHLPFFKRLLSDHLSPKEGILYHFDGNDMFFRMSYP
                     WLSGDQIVIGKVDVENYEARIRYQIQTIAREWKNIPYTVTNIKDSILIGSIPENGTIL
                     EQLILGQDLNWTLTLFQKDPKEIHKETRHKMFLLYGLVSFSLITVLFGSFFMFRFLIQ
                     ERKLLFMKSNFLSSVSHELKTPLTSIKMFAEMMARGRVQKPEKIQEYSSLIGKESTRL
                     ENLIGAILSYTRMENGTSAFKWERFDLGICAETVYNALLDITEQKGLEISSRIEKNLF
                     IMGDYTALYSLIQNLIENAIKYTNSPGSIFVQAYEENEKILFSVVDTGIGIPPSEQKN
                     IFNDFYRVGDEMTRSTKGSGLGLAIVKRVADTHKASISLQSRLGKGSTFTVRFKKAE"
     gene            complement(2558..3727)
                     /locus_tag="PLT31_02965"
     CDS             complement(2558..3727)
                     /locus_tag="PLT31_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPW94129.1"
                     /translation="MNQMKLLTLLLGLTALVTGAYFVKKGDTLWDLSETFLNDPFAWP
                     DLWENNRHIEDPHWIYPGDSIYFGENDSVSDTMPPAQPKDQNLCNSSIPDSALPKTVT
                     PVGCDENDSRGDQFENLLGNLRSKSKIEKKKKVTDEYFYQQRPESKIFNGYYQVLSPD
                     VLKLDELKNDSRWFSVQSGERSTPLLHMPENEIVVGVGYKTNSNIKKGDLVEVWQTKT
                     IEYLNPVSKNTEKTALLQIGGYAKITAIGDTLSRAILVQSFKEINIEKSKAKLKEPYS
                     AINVKGYSTINEVKVDEMGYVLYSIDPALVIGAYSYIIIDKGTKSGYNAGDGVAIWEE
                     DLSDPGLPPRLLGRGIVTRAKQEQATVLIREIYSNNRRIKLGHRISLTHKAQLVQ"
     gene            complement(3790..5004)
                     /locus_tag="PLT31_02970"
     CDS             complement(3790..5004)
                     /locus_tag="PLT31_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94130.1"
                     /translation="MKTITELSQKKDLLLDWVRKEKIPGKIQAWYSLESESTHCDFRI
                     IQIRRGSKEMLSIEPPVPGMATPEMVQAIYDQLRKEHLHILKKAIIQIWIRASGHGQF
                     GVLVQANLRSAATTKAYKSFVSFLERTFPEIVSCHHIQTYPFTLFDPAQNDSPIRIDL
                     KNDFGNSFLPIGNTPFSYHIVDWAPKCKAAWIQLPERIKNFIHPTAEDRLLEFHASSG
                     YLGASLSPYFKSVDSVDMYDSAKQMAIKNAHQLPKANMKVHRERLSEKWILSFFNIPE
                     NEGKWTILFNPPAGKPLPTGVIMATAKACPERILFISSNLEEAAKEIKRFRREGYMLR
                     KALPMDLEPGTKSFEVLFLFVPDRAGILGDKKTFSTKKEQKTNSKPKKEVKIGEIPHF
                     VQRKKPTFSRKD"
     gene            complement(5006..6727)
                     /locus_tag="PLT31_02975"
     CDS             complement(5006..6727)
                     /locus_tag="PLT31_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPW94131.1"
                     /translation="MEDPKKLINQFLKTKHSPNEVKDSLFKAAEAALSEGWTYMEAAF
                     QLGSKAQEEGLSEEESESIIRRAFASEKRTKERAPDSSEAPVIMSTQASPSVAHASPT
                     FAAGMIPLDQVLAMGLDTQSIELLQNHRIDPEALSIPWPATDWRKDLAKLVEIAFHPD
                     ETIDFKMSNTPESTSEKISNIVNQSDAIKKIMKSLDGPEGALISINATHGGPDATNES
                     YRYRYVVVDNPKMSLAKQLAYYKALNLPCIALVNTGANSVQAWVKINAADLEEYHERV
                     DFLFSTLEEQGFPVNAANKNANQMVRMPGVLRNGKQQYLIGLEQGAKDFLEWKEWVEY
                     CLDGKPLIELASDSEKPPKKDDQIIQNALRAGEFMLLTAPQKTGKSFALLDLALSICY
                     GEEWLGSTTTTNDVLFINFEFTKATFLNRLHLIASKRNIPATTSKLGFLNLRGMTLSP
                     LEIAQLIAKRIEGAKKLENHDYKVVIIDPIASVLHNPKAMRTTGPSHQILMQMVDTII
                     ALTGSAVITASNTDEFPYLETHADSVISLNPMEGCPNVFQIHGTFREFAPFLAKECSW
                     IFPRFII"
     gene            6886..8574
                     /gene="ettA"
                     /locus_tag="PLT31_02980"
     CDS             6886..8574
                     /gene="ettA"
                     /locus_tag="PLT31_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HPW94132.1"
                     /translation="MAEKTPEKFIFYMHRMTKLYPPNKEVLKDISLSFFYGAKIGIIG
                     PNGSGKSTLLRIMAGIDKEFQGEAWIEPGRTAGYLPQEPQLDPNLTVKENVMLAVKKK
                     QAILDSYNEISMRFAEELSDDEMNRLMEEQGKLQDIIDAQDLWSLERNIEIAMDALRC
                     PPGDAVVTHLSGGEKRRVALCRLLLEEPDLLLLDEPTNHLDAESVAWLERHLRDYKGS
                     VILVTHDRYFLDNVTNWILEIDRGRGIPWQGNYAEWIDQKLDRLKNEEKGESDRQKRL
                     AREQEWVKQSPKARQAKSKARLKAYEELLAADQREQIKVAQIAIPNGKRLGDKVIEAS
                     GLRKAFGDKLLFDDLSFTLPRSGIVGVIGPNGAGKTTLFKLVMGTETPDAGELKIGET
                     VEIISMEQGRDSLDDSKTVWETITDGRDEIVLGERKMNSRAYCGLFNFTGADQQKKLS
                     VLSGGERNRVLMAKNLQKPGNLLFLDEPTNDLDVETLQALEQAVLRFAGCAVIISHDR
                     WFLDRIATHILAFEGDSKVVWFEGNWSEYEADRRKRLGEDADNPKPIRYKTLSR"
     gene            complement(8652..10523)
                     /locus_tag="PLT31_02985"
     CDS             complement(8652..10523)
                     /locus_tag="PLT31_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPW94133.1"
                     /translation="MKRFILIFFLFCFVSLFANDKIKNPWYVEFSGNSLFSTFQLTDN
                     LDVPKEFGQMDTTKQDFLMKLAKEGLTGLYYAQGYFSADINLEIQREYYSLDSAQRGY
                     LFYITEGPQYTLDSIHITVKEGNRELEINKHRLKSNKKSAYNSEAIIEDLEYIKSIYR
                     KEGYLHTTIDYNELLDTLQHVVMLQVRIDPKTQVLMGKIKSKSFKPLSKSNTEAPQEE
                     GLSDTSWLNSLWRIPSESLIDGEQFSSFKNKLFSTQLFTQIKLEDSLRADGFSDVHLQ
                     VIERVPGEAKYSIFFEEYYGFGGSASLRHKNFFGSFHEFSTHLFVAQNKQEIALGYAN
                     PLLFGTALTFIPTAIRFEDRLSFNHEKITPPAYPDSVEERMEVINRGDITFGLSSKIR
                     FRGTLDSRFVNKNNDQIFKFKYENALTFDFTDDFFNPKKGIRFIPTLGAGINLQGTEM
                     IGNPYTYGELTTNLYFPIIGTLLGAISGSGGIFFNEALEDDARIFYQGGARSVRSYRF
                     RSIFAGYESVEDGDTLINTSLTPRYLRLNQELRFNIPSVSWKNWQIVQFWDWTKVMDK
                     DSDLFKEKMSASVGLGIRYHWQFLTLRLDYAFKKDFGSASPEGFAFNRFTFDLSQAF"
     gene            complement(10520..>13528)
                     /locus_tag="PLT31_02990"
     CDS             complement(10520..>13528)
                     /locus_tag="PLT31_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94134.1"
                     /translation="SFSGDSKEIIAEAFLRNEDGGEIELHGTLDFDLNFNLRGSVKDM
                     SAEFGPQIMPLDVQIHSAVKKGNSIVADIKTRRGSEVKANLSNLDSNLRIDYQARITP
                     TESWALDWTSGNVILEDITHISGYFQDGLLHAHAKIAPVPYAYGMKADSVVTDLVLNE
                     DGIIFPTINIFTPRDSLFASGEVIWAHPKQHTAWELTLPNSSAKAWIDFEDSIKISAE
                     THNIEVASIPLAKSNILNKIKGTVTASWNHNFDSNEGYAEIEASTMLDKFMIEGKMTV
                     KQNQDTIFIQNAKINHDKNAIEAEATFLIPNESTPKGILPVDLLHAWVASHEFDIPLI
                     LQPLQDSTFKSAFFSGDLAYNKQFGLQGNINFDRILFNNISEDVLSIHRVNLFTEKDK
                     AELNAYLKIGNGGWNGNTQIILSDLFNSKRHLNISHATETGGTIWTDGFIDTSLTFNG
                     SIQATGSWFLPANAGEITQTDLQIDVKAPLKKGLSGIEAQFYSDKSFYKIPSFNMTIP
                     ISLHGKMKDKVVSVANVKTKNELDEEINASVQINLDQMELDAIRFYSEKYSLTYDSIH
                     SIEIKNLFGQMEDFSDEFIISSDLFEIAYNLNSPSIGKASANLRGKLNVNIPHSKENS
                     IENSSVNGFIFIDKAVYEKEIDIDISPKSIDRLLTMFKNTLVKFRKSSIETASIAGGR
                     PTNLSIHISDSQKDSVAIVTSVAKFPLTADVWLLGTTQHPILRGDVTSSGEGFLGIEK
                     LYEFNLDYFRIYWPDVAWQKGLLDVSSSQELPYCNSTEKEEEETCPINLNIMGTVTAP
                     QVIPSSSCGNESSAASMYYNIFLGCISDENTTNLTDWNKLAGKAIGKALSSTANKTLG
                     GEYIGDIDMKIQLFNSAGTSEKDSSYFRVPISLDRWVKDLSIIFGYTQDQSENPSYDQ
                     SLELGVNYTLPVFNEKDQNQLEHLDPKLTLNGVLISKQYISNTGTSNSESRVEKNIGF
                     NYSYRFWTPCILGFGQCKDKRGPFDEEDEK"
BASE COUNT         3988 a         2334 c         2615 g         4591 t
ORIGIN      
        1 tcatcttttt taaatgcttc gtatttctta aaatctaatc gaatatcagc aaattggatt
       61 acatctggaa ccactgttgt gtttgattca atagcggttt ttaaatccgc agaacgacgt
      121 aagaaggcct tgactctcgc gagtaattca agaatagaaa atggtttggt gacatagtca
      181 tcggcaccca tttctagccc agcgacttta cttgtttctt ctgttttggc tgtaagcata
      241 ataataaaac atttaggatg cttcttacga atcactcgac atacctcaaa accacttttt
      301 ttcgggatca ttaaatctaa aatgacaaga tctggtttaa aggaatccgc tttagcaata
      361 gcttcttctc cgtcagaagc ggtttctacg atgtaatttt caagttcaaa attatcttga
      421 agccctaagc ggatgatttc ttcatcttct actattagta ttttattttc catattactc
      481 tgccttttta aacctcactg taaatgtaga accttttcct aagcgacttt gtaatgatat
      541 agacgcttta tgagtatctg cgactcgttt aacaatcgca agccctagtc cagagccttt
      601 tgtacttctt gtcatttcat caccaactcg atagaaatca ttaaaaatat ttttttgctc
      661 agaaggagga attcctatac ctgtgtctac cactgaaaat aaaatttttt cattttcttc
      721 gtaggcttga acaaaaatag aaccaggaga attcgtgtat ttaattgcat tttcaattaa
      781 attttgaatt aagctataaa gagcggtgta atctcccatt ataaaaagat ttttttctat
      841 tctagatgat atttctaatc ctttttgttc tgtaatgtct agaagtgcat tatatactgt
      901 ttctgcacaa atccctaaat caaatcgttc ccatttaaat gcagatgtac cgttttccat
      961 gcgagtgtaa cttaaaatag ctccaattaa gttttctaaa cgagtggatt cttttccaat
     1021 taaagaggaa tactcttgaa ttttttctgg cttttgaact cgtcctctgg ccatcatttc
     1081 agcaaacatt ttaatggatg ttagaggcgt ttttagctca tgagaaacgc ttgacaaaaa
     1141 gtttgatttc atgaataaaa gctttcgttc ttgaatcaaa aaacggaaca tgaaaaagga
     1201 accaaataat acggtaatta gagaaaagct aactaaaccg tataagagaa acattttatg
     1261 acgtgtttct ttatgaattt cttttgggtc tttttgaaat aaagttaaag tccagtttag
     1321 atcttggcct aaaataagtt gttctaaaat ggttccattt tctggaatag aacctatgag
     1381 aatagagtct tttatgttag tgaccgtata cggaatattt ttccattctc tagcgatggt
     1441 ttgaatttga taacgaatgc gggcttcata attttcaaca tctacttttc ctataacgat
     1501 ttgatctcct gaaagccaag gatatgacat tctaaaaaac atgtcatttc catcgaaatg
     1561 atagagaata ccttctttag gggataaatg atcggaaagt agtcttttga aaaagggaag
     1621 atgttcgtat aaaatttctg tatgggttaa ttggcgatta aagttttcac ggagattcca
     1681 gaatgcttct ctagtttctt gtgttaactt ttcaaaggaa aggatctgat taaatgtggt
     1741 ttcaaaaaaa taacgagtgg cactaatatc attaatatgg ggctgttgta aaaagcgatt
     1801 taattctaat aagcacaagt tttgagcttc ttcgtgctta ttttgttcaa caagaatttc
     1861 aaagtgtaac agttgtagag attctcttag ttgataatgc agatagcttt gaagattagg
     1921 acgttgttgc aaaagagaga tgatgtggag ggcttcatca tattttttat tttggtatgc
     1981 attacgaaga aggccaagaa ggttttgtac ttgctcgctt ctgctttgaa aaggattttg
     2041 cattaaacga agagaacgtg ctaattttac tttttcttca tttgttttat tgatttcttc
     2101 aaaaaaaagt tgttgctcta tagaggaata attcgtctga ggccatttag aactttcata
     2161 ataaaaatga gacgctatat taggataaac gattgtctct ttattgaaaa ggaaaatagc
     2221 gcttagccct tttacatttt taaaggagga ggcgtttcca aattctaata aattctgtgg
     2281 ctgttcgtaa agaaattgag atgccgcctt ggtctcttgt aatattttcc cttgttcttt
     2341 ttgaaccaca ttttctagtt cttttttaaa agacttaagg ttttcttcga aatttttttt
     2401 agctaagtag gtctcatttt gaatattacg aaaacttaaa acagaaagaa tcgcggtagg
     2461 aaggatgatt cccccaaaaa agaaagcgac gagaatcgga ttgcttttag aaaaccgcat
     2521 acgctctctt aaagattatc tgagcgattc atctttatca ttgaactaat tgggctttat
     2581 gagttaatga aatgcgatgg cctaatttaa tacgtcgatt attggagtag atctcacgaa
     2641 ttaaaacagt ggcttgttct tgttttgctc gtgtaactat tcctctacct agaagacgtg
     2701 ggggaagtcc tggatctgat aaatcttctt cccatatagc gactccatca cctgcgttgt
     2761 aaccagactt agttccttta tcaataatga tataagaata agctccgatg actaatgcag
     2821 gatcgataga atagagaaca taacccattt catctacttt cacttcattt atagtactgt
     2881 agcctttgac attaattgcg gaatatggct ctttcagttt tgccttgctc ttttcaatgt
     2941 tgatttcttt aaagctttga actaaaatgg ctctagaaag ggtatcgcca atggctgtga
     3001 tttttgcgta tccaccaatc tgaagtagag cggttttttc tgtatttttt gaaactggat
     3061 ttaggtattc aattgttttg gtttgccata cttcaactaa atcccctttt tttatattcg
     3121 agtttgtttt gtatccaact ccaacgacta tttcgttttc aggcatgtga agtagaggtg
     3181 tggaacgttc tccagactga actgaaaacc aacgagaatc atttttaagt tcatccagtt
     3241 ttaaaacatc tggtgacaaa acttggtaat agccattaaa tatttttgat tctggacgtt
     3301 gttgatagaa atactcgtca gttacttttt tctttttttc aattttagat ttgcttctta
     3361 aatttccgag caagttttca aattgatctc ctctggaatc gttttcatca cagccaacag
     3421 gggttactgt tttaggtaat gcggaatcag gaatacttga gttacaaagg ttttggtctt
     3481 ttggttgagc tggaggcata gtatcagaaa cactatcatt ttcaccaaaa tagatggagt
     3541 caccaggata aatccaatga ggatcctcaa tatggcgatt gttctcccat agatcaggcc
     3601 aagcaaaggg gtcatttaag aacgtttcgc tcaaatccca taaagtatcg ccttttttta
     3661 caaaataggc tcctgtgact aacgctgtca atccaagaag tagggttaag agtttcattt
     3721 gattcattgt gagtttacct tttgtaactc tttgtatact aagaggttac gttgaattta
     3781 tcttttaatt taatcttttc tagagaaagt aggttttttc ctttgtacaa aatgaggaat
     3841 ttctcctatt tttacctctt ttttaggttt agaattcgtt ttttgctctt tttttgttga
     3901 aaaagttttc ttatcaccta aaattcctgc acggtcaggg acaaatagaa acagtacttc
     3961 aaatgattta gtaccaggtt ctagatccat cggcaaggct tttcttaaca tataaccttc
     4021 tcttcgaaat cttttaattt ctttagcagc ttcttctaaa ttcgaagaaa taaaaagaat
     4081 tctttctgga caagctttag cagtagccat gatgactcct gtagggagag gtttccctgc
     4141 aggtgggtta aataaaatag tccatttccc ttcgttttca gggatgttga aaaaagaaag
     4201 aatccatttt tcgcttaagc gttcacgatg cactttcatg tttgcttttg gcaattgatg
     4261 tgcatttttt atagccattt gttttgccga gtcatacata tccacgctat ctacggattt
     4321 aaaataaggc gatagacttg ctcctaggta gccactactt gcgtgaaatt cgagtaaacg
     4381 atcttctgcg gttggatgta taaaattctt tattctttct ggaagttgta tccaagcggc
     4441 tttacatttt ggtgcccagt ccacaatatg ataagaaaat ggggtgtttc ctataggcaa
     4501 aaaagaattt ccaaaatcat tcttgagatc gattcttatt ggagaatcgt tttgagctgg
     4561 atcgaaaagg gtgaatgggt atgtttgaat gtggtgacaa ctaacgattt ctggaaaggt
     4621 ccgttctagg aaagaaacaa agcttttata ggctttggtt gttgctgcac ttcttaaatt
     4681 ggcttgaact agaactccaa attgaccgtg accagaagct cgaatccaga tttgaataat
     4741 ggccttctta agaatatgta agtgctcttt gcgaagttga tcgtagatag cttgaaccat
     4801 ttcaggagtg gccataccag gaactggagg ctcaatggaa agcatttctt ttgaaccacg
     4861 cctgatttga atgatccgaa aatcacagtg agtgctttcg gattccaaag aataccaagc
     4921 ttgtattttt ccagggattt tttcttttcg gacccaatcc aataggaggt cttttttttg
     4981 agataattct gtaatagttt tcattttaga ttataaatct aggaaaaatc caagaacatt
     5041 ctttggcaag gaatggcgca aactccctaa aagtaccatg aatttgaaat acattaggac
     5101 aaccttccat tggattcaat gaaatgacgc tatcggcatg tgtttctaag tagggaaatt
     5161 catccgtatt gcttgctgta ataacggcac ttcctgttaa ggcgatgatc gtatctacca
     5221 tttgcatgag aatttgatga gatggcccag tggttctcat ggctttcgga ttatgtaaaa
     5281 cagaagcaat tggatcaata atcaccactt tataatcatg attttcaagt ttttttgctc
     5341 cttcgattct tttagcgatt aactgagcaa tttctaaagg agataaagtc atgcctcgaa
     5401 gatttaagaa accgagcttg gatgttgttg cgggaatatt tcttttgctt gcgattaaat
     5461 ggagacgatt taagaacgtc gcttttgtga attcaaaatt aatgaataaa acatcgtttg
     5521 ttgttgttgt tgaaccgagc cattcttctc cataacaaat cgatagtgct aaatcaagta
     5581 atgcaaatga tttgcctgtt ttttgaggtg ctgttaagag cataaattcg ccagcccgaa
     5641 gagcattttg aataatctgg tcatcttttt taggtggttt ttcactatca ctagcaagtt
     5701 caataagcgg cttaccgtca agacagtatt ctacccattc tttccactca agaaagtctt
     5761 ttgccccttg ttctaaacca attaagtatt gctgctttcc gtttctaagg actcctggca
     5821 tacgcaccat ttggtttgcg tttttattcg cggcatttac aggaaaacct tgttcttcta
     5881 aagtggaaaa taaaaagtcg acgcgttcgt ggtattcttc taagtcggcg gcgttaattt
     5941 taacccatgc ttgtacggaa tttgctcctg tattcacaag cgcgatgcaa ggtaaattta
     6001 gagctttata ataagctagc tgttttgcta agctcatctt agggttatcg actactacat
     6061 aacgatagcg ataactctcg ttcgtggcgt ctgggccgcc gtgagttgca ttaatgctaa
     6121 ttaaagctcc ttcgggacca tcgagactct tcattatttt tttgatcgca tcagattgat
     6181 taacgatatt acttattttc tctgatgttg attctggtgt attagacatc ttgaagtcaa
     6241 ttgtttcatc tggatggaaa gcaatttcta ctaacttcgc taaatcttta cgccagtctg
     6301 tggcaggcca tggaatggaa agagcttctg gatcaatacg atggttttga agtaattcta
     6361 tggattgagt atctaatccc atcgctagga cttgatctaa tgggatcatg ccagcggcga
     6421 acgtaggcga agcatgggct acagaaggcg atgcttgtgt ggacatgatt actggagctt
     6481 ctgatgagtc aggggccctt tcttttgttc tcttttcaga ggcaaaagcc cttctaataa
     6541 ttgattcaga ttcttcttcg gaaagccctt cttcttgagc cttactacct agttgaaaag
     6601 ccgcttccat ataagtccag ccttctgata aagctgcttc tgcagcctta aagagagaat
     6661 ctttgacttc atttggggag tgttttgttt ttaagaactg atttattagt ttttttgggt
     6721 cttccatagc tagcctcttt tgtcttttaa tataaaagat ttatttcgaa tgaaccacga
     6781 ataaaaagaa attccttcct ataaaggtgt tttttataaa aacattatag gtaaccttta
     6841 ttatctttaa gtttaataag tctctttttt ttataggatt ttatcatggc tgaaaaaaca
     6901 ccagagaagt ttatttttta tatgcaccgc atgactaaat tgtatcctcc aaacaaggaa
     6961 gttttaaagg atatctcttt aagttttttc tatggagcta aaattggtat cataggcccc
     7021 aatggttctg gaaaatccac tttactgcgc attatggcgg ggattgataa agaatttcag
     7081 ggagaagctt ggattgaacc aggtcgtact gctgggtatc ttcctcaaga gcctcaatta
     7141 gacccaaatt taacggtgaa agaaaatgtg atgcttgctg tgaagaaaaa acaggccatc
     7201 cttgattctt ataacgaaat ttcaatgcga tttgccgaag aattatctga tgatgaaatg
     7261 aatcgtttaa tggaagagca gggaaaactt caagatatca ttgacgctca agatctttgg
     7321 agtcttgaac gcaatattga aattgcgatg gacgctttac gttgccctcc aggagatgct
     7381 gttgttactc atctttcggg aggagaaaaa aggcgcgtgg ctctttgtcg attactttta
     7441 gaagaaccag acttacttct tttagatgaa cctacaaacc atttagatgc tgaatctgtg
     7501 gcttggttgg aacgtcactt aagagactat aagggctctg ttatattagt cacacatgat
     7561 cgctatttct tagataatgt gactaattgg attttagaaa tcgatcgcgg tcgaggaatt
     7621 ccttggcagg gtaattacgc cgaatggatt gatcaaaaat tggatcgttt gaaaaatgaa
     7681 gaaaaagggg agtcggatcg ccagaaacgg cttgctcgag aacaagaatg ggtgaaacaa
     7741 agtccaaaag ctcgtcaagc aaagagcaag gctcgtctta aagcatacga ggaattactt
     7801 gctgcagatc agcgtgaaca gattaaggtc gctcaaattg ctatcccaaa tgggaaacgt
     7861 cttggcgata aggtaattga agcaagtggc ttgcgtaaag catttggcga taaactttta
     7921 tttgatgatt tatcctttac acttcctaga tctggaattg tcggcgtgat tggccccaat
     7981 ggagctggta aaacgacttt gtttaagctt gtcatgggaa cggaaactcc tgatgcaggc
     8041 gaattaaaaa ttggtgaaac cgttgaaatt atttcgatgg aacaaggtcg tgatagtttg
     8101 gatgattcta aaacagtttg ggaaaccatt actgatggac gagatgaaat agttcttggt
     8161 gaaaggaaaa tgaatagtcg tgcctattgt ggcttattta attttactgg agctgatcaa
     8221 cagaaaaaac tctcggtctt atctggtggt gaacgaaatc gcgtgttaat ggcaaaaaat
     8281 cttcaaaaac cagggaattt gttgttcttg gatgaaccga cgaatgactt ggatgtggaa
     8341 acgcttcagg ctttggaaca agcggttcta cgttttgctg gttgtgctgt gattatctcg
     8401 catgaccgtt ggttcttaga ccgaatagcc actcatatat tagcttttga aggagattca
     8461 aaagtagttt ggtttgaagg caactggagt gaatatgagg cagatcgtcg taaacgttta
     8521 ggtgaagatg ctgataatcc aaagcctatt cgttataaaa cgctttctcg ttaaggttgt
     8581 ttttaaggct atttaaacca tttaaaaagc tctcgttttc tacgagggct tttttagtct
     8641 agtctcagaa actagaatgc ttgagataga tcaaacgtaa aacggttaaa tgcaaatcct
     8701 tctggagatg cgcttccaaa atcttttttg aaagcgtaat ccagtcgaag ggttaagaat
     8761 tgccagtgat agcgaatacc aagacctacg cttgcagaca ttttctcctt aaataagtcg
     8821 ctgtctttat ccatgacttt tgtccaatcc cagaattgga caatttgcca gtttttccat
     8881 gaaacacttg gaatattgaa acgtaattct tgatttagtc tcaagtatct gggagttaaa
     8941 ctggtattaa ttaaggtgtc tccatcttct acagattcat agcctgcaaa gatacttcta
     9001 aagcgataac tacgcaccga tcttgcgcca ccttgataaa aaatacgggc atcatcttca
     9061 agagcttcat taaagaatat gccgccacta ccactaatgg ctccaagaag tgttcctata
     9121 atagggaagt agaggtttgt ggttaattca ccataggtat agggattccc gatcatttca
     9181 gtgccttgta agttgatacc tgcacctaat gtgggtatga atcgaatccc ttttttcggg
     9241 ttgaaaaaat catccgtaaa atcaaaagta agggcgtttt cgtatttaaa cttaaagatt
     9301 tggtcattat ttttattcac aaaacgagag tctagggtgc cacgaaatcg aatttttgaa
     9361 gataatccaa aggtgatatc accacgatta atgacttcca ttcgttcttc aacgctatca
     9421 ggataagctg gaggtgtgat cttttcatga ttaaatgaaa gacgatcttc aaatcgaatg
     9481 gcagtaggta tgaatgttaa tgctgttcca aataaaagtg ggttagcata ccctaatgcg
     9541 atttcttgct tattttgagc gacaaacagg tgtgtcgaaa attcatgaaa agagccaaaa
     9601 aagtttttat ggcgaaggct tgcgctacca ccaaatccat agtattcttc aaagaaaatg
     9661 gagtactttg cttctcctgg aacacgttca attacttgaa ggtgaacgtc ggaaaatccg
     9721 tctgctcgta atgaatcttc aagcttgatt tgagtaaata attgggtcga aaacagcttg
     9781 tttttgaaag aactaaattg ttctccatca attaaacttt ctgaaggaat tctccaaaga
     9841 gaatttaacc aagaggtatc tgaaagtcct tcttcttgag gagcttctgt atttgattta
     9901 ctaaggggtt tgaatgattt actctttatt tttcccatta aaacttgtgt tttgggatca
     9961 atacgaactt gtaacatcac gacatgttga agtgtatcta aaagctcatt gtaatcaatg
    10021 gtggtatgta aatacccttc ttttcgataa atgcttttaa tgtattctaa atcttcaata
    10081 atagcttcgg aattgtacgc acttttttta ttggatttta atcgatgctt gttgatttca
    10141 agctctctgt tcccttcttt tactgtgata tgaatcgaat ctaacgtata ttgagggcct
    10201 tctgtgatat aaaaaagata acctcgttga gcgctatcta acgaataata ttctcgttga
    10261 atttctaagt tgatatcagc actaaaataa ccttgagcat aataaagccc agtgagccct
    10321 tcttttgcaa gcttcattag gaaatcttgc tttgtcgtat ccatttgacc gaattctttt
    10381 ggtacatcta aattgtcggt caattgaaaa gtagagaata ggctattccc tgaaaactcg
    10441 acataccatg gattttttat tttatcgttt gcgaagaggg atacgaaaca aaacaagaaa
    10501 aagattaaaa taaagcgttt catttttcat cctcctcatc aaaagggcct cgtttatctt
    10561 tacattgccc aaaaccaaga atacaaggag tccaaaaacg ataagaatag ttgaaaccta
    10621 tatttttttc tacgcggctt tcagagttgc tggtacctgt atttgaaata tattgtttgg
    10681 aaataagtac gccattgagt gttaatttgg ggtctaagtg ttctagctga ttttgatcct
    10741 tttcattaaa aacagggagc gtgtaattaa cccctaattc aagtgattgg tcataagaag
    10801 ggttttcact ttgatcttgt gtgtatccga aaatgatact aagatctttt acccagcgat
    10861 cgagggaaat aggtactcta aaataactag aatccttttc agaagtccct gcggaattaa
    10921 acagttgaat tttcatatca atatcgccaa tatattcacc ccccagagtt ttatttgctg
    10981 tagaggaaag ggcttttcct atcgctttac ctgctaattt attccaatca gttaagtttg
    11041 tcgtgttttc atcggagata cagccgagaa agatgttata atacatggaa gccgctgaag
    11101 attcattgcc acaagaagaa ctcggtatga cttgtggagc tgtgactgtt cccatgatat
    11161 ttaagtttat ggggcaggtt tcttcttcct ctttttctgt tgaattacaa taaggtagtt
    11221 cttgggaact agatacatca agaagtcctt tttgccatgc cacatcaggc caatatattc
    11281 ggaagtaatc caaattaaat tcatacagtt tttcaatgcc taagaatcct tctccagaac
    11341 tcgttacatc gccacgaagg attgggtgtt gtgttgttcc aagtaaccaa acatcagccg
    11401 tcaaaggaaa ttttgcaaca cttgttacaa tagcaacgga atctttttga gagtcactga
    11461 tgtgaataga taagttggtt ggacggcctc ccgcaatgga tgctgtttct atagaagatt
    11521 ttctgaattt tactaaggtg tttttgaaca tggtgagtaa acgatcaata ctttttggcg
    11581 aaatatcaat gtctatttct ttttcataaa cggccttgtc tataaagata aatccgttga
    11641 cagaagagtt ttcaatactg ttttctttag agtgtggaat attaacattt agtttacccc
    11701 ttaaattggc cgaggcttta ccaatggagg gtgaatttaa attatacgcg atttcaaata
    11761 aatcagacga gatgataaac tcatctgaaa agtcttccat ttgcccaaat aagtttttga
    11821 tttcaattga gtggatgctg tcatatgtta aggaatattt ttcggagtag aaacgaatgg
    11881 catccagttc catttgatct aagttgattt gaacggaggc attgatttct tcatctagct
    11941 catttttagt ttttacgttt gctacagaaa ccactttgtc tttcattttt ccatggagag
    12001 aaatgggaat agtcatgtta aatgaaggta ttttgtagaa tgatttatct gaataaaatt
    12061 gagcttcaat tccactcaat ccttttttta gaggagcctt cacatcaatt tgtaaatcgg
    12121 tttgagtgat ttctcctgca tttgcaggta aaaaccaaga tccagtcgct tgaatagaac
    12181 cattgaacgt taatgaagta tcaataaaac catctgtcca tattgtacca cctgtttctg
    12241 tggcgtgaga tatatttaga tggcgttttg aattaaataa atcgcttaat ataatttgag
    12301 tatttccgtt ccagccaccg tttcctattt tgagatacgc atttagttca gctttatctt
    12361 tttctgtaaa aagattcact cgatgaatac ttaatacgtc ttctgaaatg ttattaaata
    12421 aaatgcgatc aaagttgatg tttccttgta aaccaaattg tttgttgtat gcaaggtctc
    12481 cgctaaaaaa ggcacttttg aaagtggagt cttgaagtgg ttgtaaaatg aggggaatat
    12541 cgaattcatg tgaggcaacc caagcgtgta aaagatcgac aggtaaaatt cctttaggag
    12601 tcgattcgtt tggaattagg aatgtggctt ctgcttcgat agcattttta tcgtggttaa
    12661 tttttgcgtt ttgaataaaa atggtatctt gattttgttt aaccgtcatt ttcccttcaa
    12721 tcataaattt gtctaacatg gtggatgctt ctatttcagc atacccttca ttgctatcaa
    12781 aattatgatt ccaagaagct gtgacggttc ctttaatctt attgagtata tttgattttg
    12841 ctaaaggaat ggaagcaact tcaatgttat gtgtttctgc actgattttt atactgtctt
    12901 caaaatcaat ccaagctttg gccgaactat taggtaaagt taattcccaa gcagtgtgtt
    12961 gttttggatg agcccatatg acttcacccg aggcaaataa tgaatctctt ggagtgaata
    13021 tattaattgt tggaaatatt attccatctt cgttaagcac gagatctgtg accacagaat
    13081 cggctttcat gccataagca tatgggacgg gagcaatttt tgcatgtgca tgtaaaagcc
    13141 catcttgaaa atagcccgat atatgagtaa tgtcttctag aatgacattt ccactagtcc
    13201 aatctaaagc ccaagattct gtaggcgtta ttcttgcctg atagtctatt ctgagatttg
    13261 aatctaaatt ggatagatta gcttttactt ctgagcctcg tcttgtttta atatcagcta
    13321 caatggaatt cccttttttc actgcagaat gtatttgtac gtcaagaggc ataatttgag
    13381 gaccaaattc agcactcatg tcttttacac ttccacggag attgaagttc aaatcaaaat
    13441 ccagagttcc atgaagttcg atttctccac catcttcatt acgtagaaag gcttcggcta
    13501 ttatttcctt tgagtctcct gaaaaaga
//
LOCUS       DAOOBB010000037        13484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_5897_length_13484_cov_4.495420, whole genome shotgun sequence.
ACCESSION   DAOOBB010000037 DAOOBB010000000
VERSION     DAOOBB010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 13484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13484
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5897_length_13484_cov_4.495420"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(259..1203)
                     /locus_tag="PLT31_02995"
     CDS             complement(259..1203)
                     /locus_tag="PLT31_02995"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPW94135.1"
                     /translation="MKRHLISIFSLLSVSTAFAIGGTLWNMTVDAYQVQIPCVPESCE
                     NNAGWWYPYTGDTEGGQVMSFEPISENEDGSLSLIMTDTSDGSILPNGNLIEDTGLRV
                     AISAANGSASTPAIAGIAFDWLKDGTIDITGMGGIIVDYAWTGALALQIELGWDEDKY
                     GFDTWYTTLPPSASPTNIYLPWSKFKQDGWDKNKHPLKTALENSKSIKIRVKNAAHDE
                     AKGTMTLYNFEYIGVTADISTRGNNQSLVQASVSGKTLTFSGITSETSFEIMNLQGQV
                     VKNGHANASVGLTGVNPGVYLLHVLGKNLNMTQKILVK"
     gene            1454..2803
                     /locus_tag="PLT31_03000"
     CDS             1454..2803
                     /locus_tag="PLT31_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HPW94136.1"
                     /translation="MIPSISWSKAHGVAFLSSLSKTESHSLPVAMSDPSNELLIALIQ
                     AETSQSITPHPYSLAEIQKYNSLSNPSTESILEKIQLQKNLSDTNVWESEPIIELVDT
                     ILESAMMQRVSDIHFEPFETHLSVRFRKDGLLIPHLTLPDWLKDSVILRLKVLACLDI
                     SEKRIPLDGRFRFRSPNHHCAIRISTLPTRYGEKAVLRLLLPQESKEDLESLGMPLEM
                     LTKVKLIFEKPQGIFIVTGPTGSGKTSSLYAGLRHIIQKQLNITTIEDPVEYELPGAN
                     QVQVNEKAGLHFATALRSVLRQDPDVIFVGEIRDKETACVALQAAQTGHLVLSTLHTN
                     DSVSAISRLKDLGISPPLIADAVLGVLAQRLLRKICPHCKGNHEGCKHCDFTGYKGRF
                     AVFELLEASSKLNHCIINDFSHSQILEEISFLKLTDYANLAVKEKKTTMEELKRVLG"
     gene            complement(2783..3637)
                     /gene="mtgA"
                     /locus_tag="PLT31_03005"
     CDS             complement(2783..3637)
                     /gene="mtgA"
                     /locus_tag="PLT31_03005"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HPW94137.1"
                     /translation="MNVEFKKVLVQIKRISFLIFKWIYRILNYLARGVLLALFLYAAA
                     FSIVVSYLLYRAFDYGYKIYDNVISLKYVQPEMSNYMKALLDSNPNFEIKHEFVPLDS
                     ISKHLQKAVIASEDAGFYFHPGFDVRAIAEALDANQLRGKTKFGGSTLTQQLAKNLFL
                     SGERSWDRKFKELAYALLMEKELGKDRILELYLNYAQWGKDMFGCEVACKMYYKKSCS
                     KLSLDQAINLAAVLASPGKHNPHMRQSQFMSQRRAVIYQNMFPKRDSVLVDSLKSLVA
                     PPSPQNPL"
     gene            complement(3656..4726)
                     /locus_tag="PLT31_03010"
     CDS             complement(3656..4726)
                     /locus_tag="PLT31_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HPW94138.1"
                     /translation="MQIKRDDIDKIKASLSITAVANALGIDVQRGKFRCLYPMRHAHG
                     DRTPSVSISEEKGLFTCWVCEDMRGDVFELVKQARSCSFAEALEWLQTEFMSQTQIHG
                     LTSLNKSITEPSIARPVKQAKPEIKPEERMKVVLSFLKRLSVVDGTPAARWLASRRIF
                     KPAWDKMRLRYISDYSALNKSLLSEFGLELLQKTGLYNDRGNLRYYKHPLIFPYLDEH
                     VRSLFFQARAIDSSVVPKELNLKGEIPYPYNKVILNQQPGWVFLCEGVVDTLTMISRN
                     MEAVGIPGVKSFKPEWVSLFAQKKVVLCFDQDGPGREATELVQNLFQRAQIQTALFGR
                     GIEDERLKLKEGQDINDWFNQR"
     gene            4745..5620
                     /gene="murB"
                     /locus_tag="PLT31_03015"
     CDS             4745..5620
                     /gene="murB"
                     /locus_tag="PLT31_03015"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HPW94139.1"
                     /translation="MAFVNIKENYSLRHLISFRVGGEARFFCEIETNEQFLEVKKKAI
                     QQNKPLFILGRGTNVVISDKGFDGYIVKLSRSFSELSIEDSIIKAKAATPLSRIARVS
                     IQNNLSGMHLLAGIPGTLGGAIYMNAGAYGQEISQTVIEVKSINDQGQIITRDASACS
                     FSYRHSIFQDNKECVLEASFQLTKGSKDALEKEMNTVMQKRRDSQPLEYPNAGSVFKR
                     TPQDFPGALVEKAGLKGFSIGGAMVSPKHANFIINTGNATAQNIYDLTSAIIETVFKQ
                     TGITLEREIIFLGDF"
     gene            complement(5598..6005)
                     /locus_tag="PLT31_03020"
     CDS             complement(5598..6005)
                     /locus_tag="PLT31_03020"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HPW94140.1"
                     /translation="MIEKIFIQYPFLRYVSAILIMAVLFHMSSKPGTDLPSLFYGSDK
                     ILHTIAYAALGCSFSFWFKPALWKKFPIRIALVVFLLTVLYGVSDEFHQSFTPGRFVS
                     GYDLIADGIGAILAIVAYRYYLQVVFFKNHLKR"
     gene            6043..7236
                     /locus_tag="PLT31_03025"
     CDS             6043..7236
                     /locus_tag="PLT31_03025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HPW94141.1"
                     /translation="MKAITLKAGRDKSALRRHPWVFSGAIDKVLGDPSLGDSVEIYSY
                     AGDFLGMGAYSPSSQIRARFWNFGEKKNIGEDYFSEILDLAIEIRRARGFDIQNKENA
                     FRLINAENDGIPGCIIDKYGPYFSIEILSAGAEVFRKIIFKLLVEKTGCTGILERSDS
                     EVRTKEGLPLRKEVAYGNVPTEPVLINENGVLFDIDLWNGHKTGYYLDQRDARKRIGE
                     LASGKSVLNCFCYTGGFGLYALKGGAKHVHQVDVSKEALKIARDLVLKNKLPTSQITH
                     TQADVFTYLRQCRDKGLQFDLIILDPPKFIESKDSLNKGARGYKDINLLAIKLLSPGG
                     RLATFSCSGLMEMDLFQKIIADAVEDAKRSAQIIERFGQPADHPVKTSFPEGQYLKGL
                     LIEVE"
     gene            complement(7239..8555)
                     /locus_tag="PLT31_03030"
     CDS             complement(7239..8555)
                     /locus_tag="PLT31_03030"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW94142.1"
                     /translation="MRFYHVILLLVLLIDVSFAAIDPRLEQGARFAAAGEYEKAIGEY
                     NAILATDPNNAQAYFAAAEVRFKMKDYSRALANYRLAYRYAPQMSEAYEGAARIYEVL
                     GNKKGAADERAKDPANQPPVVEESSSSVELSSSSEIPVSSSVIPPSSSSVVVSSSSIV
                     LPSSSSVAPVPSSATVAKDSSSKFSYNSPLFLKGVELFKAKKYNEVASIWREVLKQEP
                     GHPGAYYYAGLVRYELGELDKAEYNLKRAFDFPELGPKAHYYLSLIYKKKNKKDLEIA
                     ELRLYSETMSSLDPKDPALARLAELTGTTDAVDSTPKTQVDSTPAVVDAVKSTPPEVS
                     KVSTDERSIDAANVAFRKGDLATALSIYREFIENKTSDEEYSFALLQIGNIYRERRDF
                     RSAVAKYREIVESYPNSDWANEAERAWEDAVWQEKNASILPRQNKR"
     gene            complement(8656..10041)
                     /locus_tag="PLT31_03035"
     CDS             complement(8656..10041)
                     /locus_tag="PLT31_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HPW94143.1"
                     /translation="MNLDIASKIIDKGRQLGADFVEIFEEESRSSTLLLKDRKIENAS
                     ASTEYGIGIRLIYGTEVLYAFTSDDHLDTLLRMTETLAFGRNQKGSSIKTLDYQSMKR
                     VLDYSANAFKDPRLLGQHYKLDFLKRADDKARSLSDRIVQVSSSVSDICSTIQIINSE
                     GLHIENTRARTRANISVTGAKNQERLTSHEAPGALGGYEILETLSPENIAMECGERVL
                     RMLDAGYISGGQMPVVMGNGFGGVIFHEACGHPLETESIRRQASPFCGKLGEQIAHPC
                     LTAIDDGTLPGVWGSLSVDDEGTPTERTVLIENGVLKSYMSDRVGAKEVGVPLTGSAR
                     RESYKYAPVSRMRNTFIAEGKDSLAEMISSIDNGLYAAKMAGGSVNPATGEFNFAVDE
                     GYVIRQGKIAEPVRGATLIGKGHEILPRISMVGNDFDMAAGMCGASSGSVPVTVGQPS
                     IKVDQILVGGR"
     gene            complement(10045..10818)
                     /gene="hisF"
                     /locus_tag="PLT31_03040"
     CDS             complement(10045..10818)
                     /gene="hisF"
                     /locus_tag="PLT31_03040"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HPW94144.1"
                     /translation="MLSKRLIVCLDVRNRKVTKGVKFKGNIDIGDPVEMGARYSDDGV
                     DELVFYDITASAENRPCDIEMVKDIARHVFIPFAVGGGIRNLDDMRAVLLAGAEKVSI
                     NSLAVLHPEIIAEGAKAFGNQCIVLGMDAKFVGVSEKIPSGYEVYIQGGRRAMGIDAV
                     QWAIQAQELGVGEICLNSIDADGTKEGYELTITDLIAKAVQVPVIASGGAGVPEHIST
                     LFSKTSADAALVASMVHFGDYTVQGIKSAMRDAGIAVRK"
     gene            complement(10864..11841)
                     /locus_tag="PLT31_03045"
     CDS             complement(10864..11841)
                     /locus_tag="PLT31_03045"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPW94145.1"
                     /translation="MAHIGKMVFESIVRPEHRGFLLVDSLCERFTYHTREEWLEKIQS
                     GAVLVNGIVATIETRVETGNQVLYQIDNYAEPDVPTHFDVLYEDDEFLLVDKPAGVPV
                     HHTGRIFFNTFTSILRRAFDNEEITPMHRLDRDTGGIMLFSKTRDTGARFQRHLDRIL
                     LKKKYLAVVHGRFPQEIERCDLSLQEKKDSRIRLKMYPCLEGKPSTTLFQLQKVREEN
                     FRTLTGPFSFVEAELVTGRKHQIRAHLAALGYPVVADRLYGHEGFYYEKMLNALLTAE
                     DYAILGAENQMLHAYKVLLMLPYFKEARWFESKAFTPEMQEMLTVPSNV"
     gene            complement(11843..12949)
                     /locus_tag="PLT31_03050"
     CDS             complement(11843..12949)
                     /locus_tag="PLT31_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HPW94146.1"
                     /translation="MKKALEKFKHFFPALAFFIGFAWDSITLGRMVYSSDLLFLLAYY
                     FGALILVILLSASKMDSHLHAEPGWKLSKAYRVRNFFLNREWSEKWTSRFTWAVQFCF
                     GSLFSALVICYFKSSGSLASLFLVLFLTALLIGNEFLQRRYESFAVSLSFFCLLGTMF
                     LNFAIPHWVHRLGVIWFLISTFLSLFLCYGIWKLSGRPLKVFFAPIAISALLIVMYFF
                     NVIPPVPLVVKQHNVCLHFDRATYSCQVESPTILERMGLSTQSLSWQPSEGEIFFLSS
                     VFAPNKVKANIEHRWSYYNPKTEKFELTDRISSSRMAVQGGRESGFRTYTKKKNLSYG
                     RWRVETALQNGAVIGSKEFNVREGVPNGQTWILE"
     gene            complement(12960..>13484)
                     /locus_tag="PLT31_03055"
     CDS             complement(12960..>13484)
                     /locus_tag="PLT31_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nicotinamide nucleotide
                     adenylyltransferase"
                     /protein_id="HPW94147.1"
                     /translation="WFVPSPDRWDKTLIASPEERFEMLKLAVGDNPSFIVSDIEINMN
                     DYRGSYVSMQYIQETYPDYHFRLLVGADSYEGIPHWRDPLHFYGTEYNGHLLLRDFEL
                     IVFARRGYKEPDLKKHMELGYAPLFWIGREQGFEGMISSTGIRKSFLCSGEKPSGLPA
                     EVYQYILDHNLYRE"
BASE COUNT         4292 a         2774 c         2428 g         3990 t
ORIGIN      
        1 tcactactac ctctaagaca cccccaagtg atgaacgctt gaagcgatcg tttctgttca
       61 taatttagct acatatttcc ttggcgatat catttctaaa gaaaaatgga gcattctgcg
      121 ctctgtgata aagatttttt tcatttcatc atcgcctgaa ggcttatttt aaagcgagag
      181 cctaactctc ggttttgaag aaatacaaaa aaaagcccgt attaaacgag ctcttctttt
      241 agcagttttt attgctaatt atttaactag tattttctgt gtcatattga ggttcttacc
      301 tagcacatga agtaagtaaa ccccaggatt tacaccagtg agacctacag aagcattagc
      361 atgcccattc ttaaccactt gaccttgaag gttcattatt tcaaacgagg tttcagaagt
      421 gataccgcta aatgtcaagg tttttcctga cacagaagct tgaacgagtg attgattatt
      481 acccctagta gagatatcgg cagttactcc tatgtattca aaattataaa gggtcatagt
      541 tcctttggct tcatcatggg cggcattttt aactctaatt ttaattgatt ttgaattttc
      601 taaagcagtt ttcaaaggat gtttgttttt gtcccaacca tcttgtttga attttgacca
      661 aggaagatag atattggttg gacttgcaga tggaggtaga gtagtatacc atgtgtcaaa
      721 gccatatttg tcctcatccc aaccaagttc tatttgtagg gctaaagccc cagtccaagc
      781 atagtctaca atgataccgc ccatacctgt gatatcgatt gtaccgtcct ttaaccaatc
      841 gaaagcgata cctgctatcg ctggcgttga tgcagagcca ttagcagcag aaatagccac
      901 ccgtaaacca gtatcttcaa tgaggttacc gttaggaagg attgatccat cactcgtatc
      961 tgtcataatc aaacttaagg aaccatcttc attttctgag atgggttcaa atgacatcac
     1021 ttggccacct tcggtatctc ctgtgtatgg gtaccaccaa ccagcattat tttcacacga
     1081 ttcaggaaca caaggtatct gaacctgata agcatcaaca gtcatattcc atagagtacc
     1141 acctatggca aatgctgttg aaacggataa tagactaaaa atagaaatca aatgcctttt
     1201 cataacttct cctaggtatt tatttaatat taattacaat ttgaatatat ataatttttg
     1261 atttattaca ataaattatt ttttaaaaat gtttttgatt taatctacgg tatactattt
     1321 caagacgtat acttcaataa tccatcattt gaggggatcc tcaatccatc attatttgtt
     1381 atataaatta tatcgtaaag aacatataaa gggttttgtg cttttttaga acatcttttt
     1441 ataaattaat ctaatgatcc cttctatttc ttggagcaag gcgcatggcg tcgccttttt
     1501 gtcgtctcta tcgaagacgg aaagtcactc tctacctgta gcgatgagcg acccttctaa
     1561 tgagttgtta atcgccttga ttcaagcaga aacaagccaa agtattactc cacaccccta
     1621 ttctcttgca gagattcaga aatacaattc tttaagtaac ccttcgacgg aatcgatttt
     1681 ggaaaagatt caattgcaaa aaaatttaag tgatacgaat gtttgggaat cggaaccgat
     1741 tatagaactt gtggatacaa ttctcgaatc cgcgatgatg caaagggttt ctgacattca
     1801 ttttgaacca tttgaaacgc atctttctgt tcgttttcga aaagatggtc ttttgattcc
     1861 ccacttaacg ctccctgatt ggctaaaaga ttctgtgatt ttgaggctca aggttttagc
     1921 atgccttgat atttcagaaa aaaggattcc gcttgacgga cgctttcgat ttagatcacc
     1981 aaaccaccat tgcgccattc gcataagtac tctccctact cgctacggag aaaaagcggt
     2041 tttgcgttta ttgctacctc aagagtcgaa agaagattta gagtctttag ggatgcctct
     2101 tgagatgtta acaaaagtaa agctcatttt tgaaaaacct caaggaattt ttattgtcac
     2161 agggcccact ggaagtggaa aaacgagttc tctttatgca ggcttacgtc acattattca
     2221 aaagcagtta aacatcacga caatcgaaga ccccgtggaa tatgagcttc ctggagcaaa
     2281 tcaagtgcaa gtgaatgaaa aagcggggct tcattttgca acggctcttc gctctgtttt
     2341 aaggcaagat ccagatgtga tttttgtggg tgaaattcga gataaagaga cggcatgcgt
     2401 tgctttacaa gcagcgcaaa caggtcactt ggtgttaagc acccttcaca ccaatgactc
     2461 tgtttcggca atctcaagac taaaggattt agggatttca ccacctttga ttgccgatgc
     2521 cgtcctcggc gttcttgcgc aacgactttt aagaaaaatt tgccctcatt gtaaaggaaa
     2581 tcacgaaggt tgtaagcact gtgattttac tgggtacaag ggacgttttg ccgttttcga
     2641 attattagaa gcgagctcaa aactcaatca ttgtattatc aatgattttt ctcattcaca
     2701 aattcttgaa gaaatctcat ttcttaaact gactgattat gcaaatctcg ctgtgaaaga
     2761 aaagaaaaca acaatggaag aattaaagag ggttttgggg tgaaggtggc gcaaccaaag
     2821 attttaagga atctactaaa acactatctc gtttggggaa catgttttga tagatcacag
     2881 cacgacgttg tgacataaat tgactctgtc gcatatgtgg attatgtttc ccagggctag
     2941 caagcactgc cgctaaatta atagcttggt caagactcaa tttagagcaa ctcttcttgt
     3001 aatacatttt acaagcaacc tcgcagccaa acatatcttt tccccactgt gcgtaattca
     3061 gatataattc taaaatacga tctttcccta attctttttc cattaacaag gcgtatgcga
     3121 gttccttaaa ttttcgatcc caagaacgtt caccgcttaa gaaaagattt ttggctaatt
     3181 gctgtgttaa agtactacca ccaaattttg tttttcctct tagttgattt gcatctaaag
     3241 cttctgcgat cgcccgcaca tcaaagcctg gatggaaata aaagccagcg tcctcacttg
     3301 cgataaccgc tttctgaaga tgtttactaa tgctatctaa aggtacaaat tcatgtttaa
     3361 tttcaaaatt aggatttgaa tccagaagag ctttcatata attactcatc tctggttgta
     3421 cgtatttcaa cgaaattaca ttatcgtaaa ttttatatcc ataatcaaaa gctcgatata
     3481 ataagtaaga aacaacaatg gaaaacgctg ccgcatataa aaataaggct aacaatacgc
     3541 cacgcgctag ataatttaat atccgataaa tccacttaaa tatgagaaaa gaaattcgtt
     3601 tgatctgaac aagtactttt ttaaactcta cattcatatt cctaaaccta aataattagc
     3661 gctgattgaa ccagtcatta atatcttgac cttctttcaa ttttaaacgt tcgtcttcaa
     3721 taccgcgacc aaaaagagcc gtttgaattt gagctcgctg aaataagttt tgaactaatt
     3781 ccgttgcttc tcggcctggc ccatcttgat caaaacaaag cactactttt ttttgagcaa
     3841 atagagaaac ccattctggt ttaaacgatt ttactccagg aatcccaact gcctccatat
     3901 ttctggaaat catagtgaga gtatctacaa ctccttcgca aagaaaaacc caaccaggtt
     3961 gttgatttaa aatcacctta ttataaggat aaggtatttc accttttaga tttaattctt
     4021 ttggaacaac gctcgaatca atggctcttg cttgaaagaa aagggagcgg acatgctcat
     4081 ctaaatacgg aaaaattaga ggatgcttat aataacgtag gtttcctcga tcattataca
     4141 atcctgtttt ttgtaaaagt tctaagccaa actcagaaag taacgactta ttaagggcac
     4201 tataatctga aatataacga agacgcattt tatcccaagc aggtttaaaa atacgacgac
     4261 tagctaacca acgagccgca ggcgttccgt cgacaactga aaggcgcttt aagaaagata
     4321 aaaccacctt catccgttct tcaggcttaa tttctggctt cgcttgtttt acaggccttg
     4381 ctatacttgg ttctgtaatt gatttgttta aggaagttaa cccatggatt tgagtctgag
     4441 acatgaactc tgtttgtaac cactctaaag cctctgcaaa agaacaacta cgagcttgtt
     4501 tcactagttc aaaaacatcc cctcgcatat cttcacaaac ccaacacgta aaaaggccct
     4561 tctcctcaga aatagaaacg gaaggcgtac gatctccatg agcatggcgc atgggatata
     4621 agcaacggaa tttgcctctt tgcacatcaa ttccaagtgc attagcgact gccgtgatcg
     4681 atagggacgc ttttatttta tctatgtcat cgcgtttgat ttgcatcttt ctaaattatt
     4741 tgatatggct ttcgttaata taaaagaaaa ttactctctg cgtcatttaa tttcatttag
     4801 agtgggcggt gaagcgcgat ttttttgtga aatagaaaca aatgaacaat ttttagaagt
     4861 taaaaaaaaa gcgattcaac aaaacaagcc tctttttatt cttggacgcg gtacaaacgt
     4921 tgttatttct gacaaaggct ttgatggcta tatcgtgaag ctgtcacgtt ctttttctga
     4981 attatccata gaagactcta tcataaaagc gaaagccgcg actcctttat cgagaatagc
     5041 tcgagtatcc attcaaaata atctcagtgg tatgcatttg ctcgcaggta tcccaggaac
     5101 attagggggt gctatctaca tgaatgcagg ggcatacggg caagaaattt ctcaaacggt
     5161 aatagaagtc aaaagcatca atgatcaagg gcaaatcatt acccgagacg cttctgcctg
     5221 ttctttttct tataggcact ctattttcca agataataaa gaatgcgttc tagaggcctc
     5281 ctttcaattg actaaaggct ctaaagatgc tctagaaaaa gaaatgaata cagtgatgca
     5341 aaaaagaaga gattctcaac ctctagaata ccccaatgca ggttctgttt ttaaaagaac
     5401 tcctcaagat tttcctggag ctttggttga aaaagcaggg ctcaaaggat tttctattgg
     5461 tggtgccatg gtttctccaa aacatgcgaa ctttataatc aatacaggga atgccaccgc
     5521 tcaaaacatc tatgatttaa caagcgcaat cattgaaact gtatttaagc aaacagggat
     5581 tactctcgag cgagaaatca tctttttagg tgatttttaa aaaagactac ttgtaaataa
     5641 tagcgataag cgacaatagc aagaatcgct ccaattccat cggctattaa atcatagcca
     5701 gagacaaatc ggcctggtgt aaagctttgg tgaaattcat cagagacacc atatagtacg
     5761 gttaataaaa atactacaag agcaattcga ataggaaact ttttccaaag agctggctta
     5821 aaccaaaacg agaaagaaca ccctaaagca gcataggcta tcgtatggag tatcttatct
     5881 gagccataaa ataacgaagg taaatccgtt cctggttttg aagacatatg gaataaaaca
     5941 gccattatga gaatagcaga gacatatcgt aaaaatggat attgaataaa gattttttct
     6001 atcatagcca tattataata gaaaattcta acttttcttt ttatgaaagc cattacatta
     6061 aaagctggac gcgataaatc tgcattacgc cgtcatcctt gggtttttag cggagcgatc
     6121 gacaaagtcc ttggagaccc aagtcttggg gattccgttg aaatttattc ttatgctggg
     6181 gatttcttag gcatgggagc ttattctcct tcttctcaaa ttagagctcg attctggaat
     6241 tttggagaga agaaaaacat aggagaagat tacttctctg aaatcttaga ccttgctata
     6301 gaaatacgcc gtgctagagg ctttgatatt caaaataaag agaatgcgtt tcgtttgatt
     6361 aatgctgaaa acgatgggat tcctggttgt attatcgata aatatggtcc ttacttttcg
     6421 attgaaattt tatccgctgg cgcagaagtt ttccgaaaaa tcattttcaa attactcgtc
     6481 gagaaaacag gctgtacagg gattttagaa cgatccgatt cagaagttcg taccaaagaa
     6541 ggtttacctt tacgcaaaga agtggcttat gggaatgtgc ctactgaacc tgttctgatt
     6601 aatgaaaacg gcgttctctt tgatattgat ctttggaacg ggcataaaac aggctactac
     6661 ttagatcaac gtgatgctag aaaaagaatc ggagaattag catctgggaa aagtgttttg
     6721 aactgtttct gctatacagg aggctttggt ctttacgcgc taaaaggcgg tgctaaacac
     6781 gttcaccaag tcgatgtatc taaagaagct ctgaagattg cacgtgattt agtattaaaa
     6841 aacaagctcc ctacaagcca aattacacat acacaagccg atgtctttac ctacttaagg
     6901 caatgtcgag ataaagggct tcaatttgat ttaatcatcc ttgatccgcc taaatttata
     6961 gagtctaaag acagcttaaa taaaggagct cgtgggtata aagacattaa ccttctcgcg
     7021 atcaagcttt taagccctgg tgggcgtctt gctactttta gttgttctgg tttaatggaa
     7081 atggatctat ttcagaaaat cattgccgat gcggtagaag atgctaaacg aagtgctcaa
     7141 attatagaac gttttgggca accagcagat caccctgtaa aaacgtcctt ccctgaaggg
     7201 caatatttga aaggtctttt aatcgaagtg gaataaaact agcgcttgtt ttgacgtggt
     7261 aatatagaag cattcttttc ttgccatacc gcatcttccc aagctctttc tgcttcattt
     7321 gcccagtctg aattagggta gctctcaaca atttctctat acttcgcgac tgcagaacgg
     7381 aaatcacgac gttctctgta tatgtttcca atttgtaata aagcgaatga atactcttca
     7441 tcgcttgttt tattttcaat aaattctcga taaatagaaa gagctgttgc taaatcgccc
     7501 tttctaaaag caacatttgc tgcatcaata gaacgttcat ctgtagaaac cttagaaacc
     7561 tctggaggag ttgatttcac agcatctacg actgctggcg tagaatctac ttgtgtcttt
     7621 ggagtcgaat ctactgcatc tgtagtacct gttaattccg caagccttgc aagcgcaggg
     7681 tctttaggat ctagagatga catagtttct gaataaagac gaagctcagc aatttctaaa
     7741 tctttcttat ttttcttttt ataaataaga cttaaataat aatgcgcttt aggccctaat
     7801 tcaggaaaat caaaagcacg ttttaaatta tactctgctt tatctaactc ccctaattca
     7861 taacggacta agccagcata gtaataagct ccaggatgcc ctggttcttg ttttaagact
     7921 tctctccaaa tagaagccac ctcattatat tttttggctt taaaaagttc aacgcctttt
     7981 aagaatagag ggctattata agaaaacttt gaagaagagt ctttagcaac cgttgctgaa
     8041 gaagggactg gagcgacaga agaagaagaa ggtagaacaa ttgaagacga agaaacgact
     8101 acagaagaag aggaaggagg aatcactgaa gaggatacag gaatttctga agacgatgat
     8161 agttccactg aagaagacga ttcttcaaca acaggaggtt ggttagcagg gtctttcgca
     8221 cgttcgtctg cggccccttt tttatttcct aatacttcat aaattcgagc ggcaccttca
     8281 taagcctcac tcatctgagg agcatagcga taagctaaac ggtaattagc tagtgctcgg
     8341 ctataatctt tcattttaaa acggacttct gcagcagcaa aataggcctg tgcattattg
     8401 ggatccgttg caagaatagc gttgtattct ccaatagctt tttcatactc gcctgcagca
     8461 gcaaaacggg ctccttgttc taaacgagga tctattgctg caaaggaaac atcgatcagc
     8521 aaaactaaaa gtaagataac atgataaaag cgcataacta aataatacta aaaacaatat
     8581 taaaattttt aacgatttgc catttttttg ttctaaaaga acctttatct tcttttcaaa
     8641 caaagggtag ctgtattaac gcccacccac aagaatctga tctactttaa tggaaggctg
     8701 gcccacagtg actggaactg aacccgaaga agcgccacac atgcctgctg ccatatcaaa
     8761 atcattgccg accatactga ttcttggtaa aatctcgtgc ccttttccaa taagagtggc
     8821 gccacgcact ggttctgcaa ttttcccttg gcgaatcaca tagccttcat ctacagcaaa
     8881 attaaactcg cctgttgcag gattcacaga accacctgcc attttagctg catacaagcc
     8941 gttatcaatg cttgaaatca tttctgctaa agagtcttta ccctctgcaa taaaagtatt
     9001 tctcatgcgg ctaacaggag cgtatttata agattctcga cgagcacttc ctgtaagagg
     9061 aacacccact tcttttgcgc ccactcggtc actcatgtat gattttaaaa ctccattttc
     9121 aattaaaacg gtacgttctg ttggagtgcc ttcatcatca acagaaaggc ttccccaaac
     9181 gcctggcaaa gtaccatcat cgatagcggt taaacaaggg tgtgcaattt gttcgcctag
     9241 ttttccacag aaagggcttg cttgacgtcg aatagattct gtttctaaag gatggccaca
     9301 agcctcatgg aaaatcacgc caccaaaacc attccccatc acgacaggca tttgaccgcc
     9361 actgatgtag cccgcatcta acatgcgtaa aacacgttct ccacattcca tagcaatatt
     9421 ttcaggagaa agtgtttcaa gaatttcata accgcctaag gcgccaggag cttcatgaga
     9481 agttaaacgt tcttgatttt ttgccccagt aacactgata tttgcccgtg tacgagctct
     9541 tgtattttca atatgaagcc cttcactatt gataatctga atcgttgaac aaatatcaga
     9601 aacagaggaa cttacttgaa caattcgatc agacaaagac cgagctttgt catctgctct
     9661 tttaagaaaa tcgagtttgt aatgttgccc taataatcta ggatctttaa acgcatttgc
     9721 tgaataatct aaaacacgtt tcatcgactg ataatcaagt gttttgatag aagagccctt
     9781 ttgatttctg ccaaaagcaa gcgtttccgt catgcgaagt aaagtatcca gatgatcatc
     9841 actagtaaaa gcgtaaagaa cttctgtacc gtaaattaaa cgaatgccaa tgccatattc
     9901 agtacttgcg gaagcgtttt caattttacg atcttttaaa agcagagtgg aagagcgaga
     9961 ttcttcttca aagatctcta caaaatccgc tcctaattga cggcctttat caatgatttt
    10021 tgaggcaata tctaaattca taatttattt ccgtaccgca ataccagcat cacgcatggc
    10081 tgatttaatt ccttgtactg tataatctcc aaaatggacc atcgatgcga caagggctgc
    10141 atctgcagaa gtcttagaaa atagcgtaga aatatgctca ggaacccctg ctccaccgct
    10201 tgcaatgaca ggaacctgta cagctttggc aatcaaatca gtaattgtaa gttcataacc
    10261 ctcttttgtt ccatcggcat caatcgaatt aaggcaaatt tcccctacgc ccaattcttg
    10321 cgcttgaatt gcccattgaa cggcatcgat tcccatggct cgcctaccac cttgaatata
    10381 aacttcatag ccactaggta ttttttcaga aacgcctaca aatttggcat ccatcccaag
    10441 aacaatacac tgatttccaa acgcttttgc gccttcggca ataatttcag ggtgaagcac
    10501 agcaaggcta ttgatactta ctttttcagc accagctaaa agcaccgcac gcatatcatc
    10561 gagattacga atcccaccac caacagcaaa cggaataaaa acatggcgag caatatcttt
    10621 caccatttct atatcacatg gacgattttc agcacttgct gtaatatcat aaaagactaa
    10681 ttcatctacc ccatcatcac tgtatcgggc ccccatttct acgggatccc caatatcaat
    10741 atttccttta aacttgacac cttttgtcac ttttctattt cgaacatcta aacaaacaat
    10801 taatcttttg gataacataa taagtcctat ttttttcaaa tatatctttt cgatgcatct
    10861 ctgtcataca ttcgagggca cggttaacat ttcttgcatc tcaggggtaa aagccttact
    10921 ttcaaaccaa cgagcttcct taaaataagg tagcatcaaa aggactttgt aagcatgaag
    10981 catttgattt tcggcaccta aaatggcata atcttctgca gtcaaaagcg cattcaacat
    11041 tttctcgtaa taaaagcctt cgtgcccata aaggcgatcg gcaacgactg gatatccaag
    11101 ggctgcaaga tgagcacgaa tttgatgctt acgtcccgtc actagttctg cttccacaaa
    11161 agaaaaaggg cctgtaagag tcctaaaatt ttcttctctt actttttgca attgaaataa
    11221 ggtggtcgag ggttttcctt ctaagcaggg gtacattttt aggcgaatgc gactgtcttt
    11281 cttttcttga agagataaat cacaacgttc tatctcttga ggaaatcgcc catgaaccac
    11341 ggccaagtat tttttcttta gtaaaatccg atctaaatgc ctttggaaac gagctcctgt
    11401 atctcttgtc ttcgaaaaga gcatgattcc accagtatcc ctatctaagc gatgcatggg
    11461 agtaatctct tcattgtcaa aagcacgacg aagaatactc gtaaacgtat tgaaaaaaat
    11521 ccgtcccgtg tggtgtactg ggactccagc tggtttatct acaagcaaaa attcatcatc
    11581 ttcgtatagt acatcaaaat gcgtcggaac atctggttct gcataattat caatttgata
    11641 aagtacttga tttcctgttt cgactcgcgt ttctattgta gcgacaatcc cattaactaa
    11701 gacagcccca gactgaattt tttcaagcca ttcttcacga gtgtgataag taaaacgttc
    11761 acaaagagaa tcgactaata aaaagccgcg atgttctggt cgaacgatac tttcaaaaac
    11821 catttttccg atatgagcca tattactcga gtatccaagt ttgtccgtta ggaacgcctt
    11881 ctcggacgtt aaattcttta gatccaatga cggctccatt ttgaagtgct gtttccactc
    11941 gccaacgccc gtaacttaaa tttttctttt ttgtataagt tctaaaacca ctttctcgcc
    12001 ctccttgcac cgccatgcgt gaagaggaaa tacgatcggt aagttcaaat ttttctgtct
    12061 taggattata ataagaccaa cgatgttcaa tatttgcttt cactttatta ggagcaaaaa
    12121 cagaacttaa gaaaaagatt tccccttctg atggttgcca actcaagctt tgagtcgata
    12181 agcccattcg ttctaaaata gttggagatt ccacttggca agaatacgtg gctcgatcaa
    12241 aatgaagaca cacattatgt tgttttacta ctaaaggaac aggaggaatc acattgaaaa
    12301 aatacatgac gattaataaa gcgctgatcg ctataggtgc aaaaaatact ttcaaaggac
    12361 gccctgagag tttccatatt ccatagcata aaaaaagcga taaaaaggta ctaatcaaaa
    12421 accaaataac cccgagacga tgtacccaat gaggaatcgc aaaattcaaa aacatcgtcc
    12481 ctaataagca aaaaaaggaa aggctcacgg caaaactttc ataacgacgt tgtaaaaatt
    12541 catttccaat taaaagagct gttaaaaata aaacaaggaa taacgaagcc aatgagccac
    12601 tgcttttgaa ataacaaatc actaaagcac taaataagct accaaagcaa aattgaacag
    12661 cccaagtaaa acgagaagtc catttttcac tccattctcg attcaaaaaa aaatttctaa
    12721 ctcgataggc tttagagagt ttccatcctg gttctgcatg caaatgagaa tccatttttg
    12781 aagcagataa caagatcact aaaatcaacg ccccaaaata ataagccaat aaaaaaagta
    12841 aatccgaact atagaccatt cgaccaagcg taatggaatc ccaagcaaat cctataaaaa
    12901 aggctagtgc tggaaaaaaa tgtttgaact tttctaaagc ttttttcatt cttgtggatt
    12961 tactcccgat acaaattatg atctaaaata tattgataaa cttcagcggg tagaccacta
    13021 ggcttttcac ccgagcataa aaaggacttt cttatccctg tactcgaaat catgccttca
    13081 aagccttgct cacgaccgat ccaaaatagc ggagcataac ctaattccat atgttttttt
    13141 agatctggtt ctttatagcc acgacgagca aatacaatta attcaaaatc tcgaagtaat
    13201 aaatgaccat tatactcagt tccataaaaa tgaagaggat ctcgccaatg aggaattccc
    13261 tcataactat cagcgccaac taataaacgg aaatggtagt ctggatatgt ctcttgaata
    13321 tattgcatcg aaacataaga cccgcggtaa tcattcatat tgatttcaat atcagaaaca
    13381 ataaacgaag gattatcacc tacagctaac ttaagcattt caaagcgttc ttcaggagaa
    13441 gcaattaatg ttttatccca acgatcagga gaaggcacaa acca
//
LOCUS       DAOOBB010000038        13234 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_6037_length_13234_cov_6.817816, whole genome shotgun sequence.
ACCESSION   DAOOBB010000038 DAOOBB010000000
VERSION     DAOOBB010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 13234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13234
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6037_length_13234_cov_6.817816"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..367
                     /locus_tag="PLT31_03060"
     CDS             227..367
                     /locus_tag="PLT31_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94148.1"
                     /translation="MKEQNRKNKLDELEKEINALKERVTKIEASFEVKDKEEEKPKTS
                     FM"
     gene            369..1031
                     /locus_tag="PLT31_03065"
     CDS             369..1031
                     /locus_tag="PLT31_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94149.1"
                     /translation="MLTDKDIKEGQKTRQEMLNAFRASRKMVSWKRRGVAKELPERKL
                     INLRRDYYNWIKENYFPILKHPSFLDQNDPDFLLNAWRSFCTMSLIRCSNHCNFLLSI
                     YPESAKYFIEIGKIKKILPLRSSFVDTYKEGDDSYSKERVKVEKDFAGWFQEAKEQIK
                     TLLAQDCYFFGNVKNNSPQYFMEKFVVDKENKEDKDNESKTVQVDFNIISRQSNANEN
                     KS"
     gene            1015..2286
                     /locus_tag="PLT31_03070"
     CDS             1015..2286
                     /locus_tag="PLT31_03070"
                     /inference="COORDINATES: protein motif:HMM:NF016359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HPW94150.1"
                     /translation="MKIKVSVDISEQQSRFILSEKKFTAFVGGRGSGKTFILGHRAAG
                     RAANGRNQLVVGRTYKELRQTIIPSVKNALELYRIPFKENKNDNIITLKKSKILFLSG
                     ENPEAIRSYTDYHDGYIDEAAWQKQMVMKNLSMCLRGKDVLNPSVSFTTTPRGGSWFN
                     LFVTKSNQEKLELIKCTTFDNPFLSEDSLELFKEALSGDEKLARQELYADMLSESPIN
                     AVIPDNLIFMSDVPLDLPVSIGIDVAREGVDNTFIVVSNEKGVVESNKFSNLDGINLY
                     LNFEKIAKKYKTLCSVNVDNTGGWASSFLDIVKTTDYRDKVFGINFASSASNNIYANK
                     RAEMLFKLRDLCEKKTFSLKNCQDVVSEINATTYFLNGSGQRQIIDKKLIKKDIGHSP
                     DELDAVSLSVLNGFNLHTNEARYSPLPQQGW"
     gene            2289..4163
                     /locus_tag="PLT31_03075"
     CDS             2289..4163
                     /locus_tag="PLT31_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94151.1"
                     /translation="MNGIVKEFIDFANKAKDKFKSEIDEMKIDRDIFSSTKVWDEIDE
                     KVRGKSRDKSTVNPLQKYKNAICNSFNRYPYDVEVVGQAPEIIKEKLKKISVDSGLNN
                     IIMQCVSDSVIMGKGFAFITTNGNEIQINYSDDPSQVIIDSESKAIDGSDIQKIAFVD
                     KMSYEKIKQTFASFTLTEDELKASKNLDVGCWESGKYSYNVISYFTLNESGVTLYRII
                     GDEVVETIVYEGLKKIPVVPLYANEFWKEGSRHYKGIVRDVRDLIKIINYSYSALKER
                     LACPLIPKTRVSFESVEGYLEDYTQANKSTAGVERYKEWSVDGRKLTAPITEYPELKS
                     NDLLVVIGNAKSEISDIIGVPQSGLAFETDSAQATATEVLLRSQANVNNVSHYYQNAK
                     SSLKNLMSICLDLLCYVEGVENTFEIAVTNGPETYVRKEMLRQQLMATQSLVPDAVKP
                     LIMAEVVKTLEIENAEALSNAILLTLPEELRPVNKTPQALALELVQSKQQIEQLQQAL
                     QQLSEQNKQLQETINTDVINSQNQLLMVRVQNESSLKTKLIEMQQRNKEFELNYQLEI
                     AKVDASQREKLMEQANEQAKIQNTLRQTALKEIELAEKMRLEEEQKRSELLALVLNKD
                     "
     gene            4239..5171
                     /locus_tag="PLT31_03080"
     CDS             4239..5171
                     /locus_tag="PLT31_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94152.1"
                     /translation="MAEEQDIINKYRGISSNDTEQKTEEPVEEQTQTPTEETETPEKK
                     PVEGESVETPEEKNSEGEKEFKNNSSPYVERIIKDRLARQAKKHQRELEALRAELQSL
                     KKKEEDPEFTRDDFIDEEEFERFKADKLKKSIKTDVMKEFESYQREREAERAQQEKVN
                     ATIANFLKTPEELQEWKSRLEDFEEDYSDFLESEQGQEMSSFMINSSVFPVMFDLIAR
                     NPSVVDKLSSLSTKEVYFNLKQLEDAILKKITGIKEAQQQENKNAEEQKPKRSLPNSG
                     KFGGSSSSTSSRLDPNSKDFDAKEYLKRKYPNQY"
     gene            5186..>5817
                     /locus_tag="PLT31_03085"
     CDS             5186..>5817
                     /locus_tag="PLT31_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94153.1"
                     /translation="MANLSTNITTAQLDILSVAVEKYAPILEDVRSSQKGLKGRTGGL
                     LRVVIPDSGSVVITENGLRDISAVTLDNAEFSKDLRISSANTAFSATALERVTNVDDF
                     DKEIVQPRAVNYGETVNETIIDKAYTVAGIAQTAALATLEFDALATTAGKLRENRATN
                     LVGYMSPTVAAKLGSKGANGSFLPPAILEPMYKDCQIGRFANVQWKESKMP"
     assembly_gap    5818..5917
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5918..6220
                     /locus_tag="PLT31_03090"
     CDS             <5918..6220
                     /locus_tag="PLT31_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94154.1"
                     /translation="HANVSVMPVATNRPTPVNCGAAKTYSVVFVFEKGNIEYDAVELN
                     TAGFESVSVSGIDSKIKTTALVSGDINTLTAKYRIDSAFVTGGIDDRRAALLFVEI"
     gene            6338..7009
                     /locus_tag="PLT31_03095"
     CDS             6338..7009
                     /locus_tag="PLT31_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94155.1"
                     /translation="MTIRDIITLIAADINYTSGNNSVGLEEANRILSLMNRCINIYNT
                     QGLLSFNYHSETPQKINGDYFVSNGIDVAALYVLCNSSKLRIKQVQLTSLYELTNSGV
                     MPSLFSIKRNIDVNGVRMIQLFFDTKNVSYELEAVIKEDLPAFNLNDEFTLPPEYQNL
                     LISDVQLRLLVNDDISPSSLLYIEKKREFEEVKKLIKEANFKNYDFGEYAISKFDKFN
                     AGLFL"
     gene            7006..8694
                     /locus_tag="PLT31_03100"
     CDS             7006..8694
                     /locus_tag="PLT31_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94156.1"
                     /translation="MKKILNSFTGGSSKYKDLDFVSHEENFNMFPETLESNEHYTNKV
                     LKSLTGSRTILSQLGGFCRGLYIASTSPLTSYNAGTPLLYGVYGSKVYRIYNNFSYDY
                     IGDVADNSEPVSFAETSGVPAHLCICSSFNIYTINLETESSLVSVDVMELPKKAGELV
                     SIRPTMITALNYRIICNDKDSDYFYYSELGKPNGINNNYAFYKYMTRYTFMKKDGTLV
                     TADDNQYYPPSEGSYVEGTLVTEDVWMGSLNYIKAEFRSDNIVAIKAMDDYLFVIGYS
                     SYQVYRWQDNINIPFITSTKNSSIGCKAPYSVSSINNKLIFLGASSVGTNAIWVSDGQ
                     GIEKISSAWIEEQIESFTRTDDAFSFCYVDGKHTFYVISFPSANRTYCYDFDEKEWHT
                     RATRDINNEQKCWFPAFAVKYSDKIIMGAFNEDKLIYLDKNKYTDYNDKVIERSRTTG
                     IIINNFKKVIIHSLELIISSGKTNVEKEYDDQMNGATPEGYNPNVMLITSADGGYTWG
                     GEKWAKAGRIGEYNSRCIFRNLGGLRRIAFKVTFTDPAPFNISKAIIDYTECGR"
     gene            8695..9006
                     /locus_tag="PLT31_03105"
     CDS             8695..9006
                     /locus_tag="PLT31_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94157.1"
                     /translation="MITSNVINPYSSETKDLLPLVLIKNGMVGFLQSDGLINLSTIKK
                     LNLPNAVYDYTLDFNFYQKINGVNLYQRTNKIYICKEQTSLDISLVGLAFIQAKLILK
                     Q"
     gene            9015..9827
                     /locus_tag="PLT31_03110"
     CDS             9015..9827
                     /locus_tag="PLT31_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94158.1"
                     /translation="MSIGSSINSAISGVTDAFGLTNVKGKKKAYDNAASMLQDTLAKS
                     GKTYSQILESIKGTGRSLQDQLGGSASVSDWINSIKEAGNKDYSVDSSKVSDFDWDKT
                     VSDYLDPNASYMIDQATQAAQNTLAGQGGLFSGGAGQQLQAVASDKARELYGDAQEQM
                     NKEKSFDYNKLLDELNIEAGNLGREQSQDMAYSSNLGNVANAYQTSVRDTQEGVNNAL
                     LSQLQNDSSIQQALANLGISEASAPTALGSIFGDVLGFASAFIPQKGVKENA"
     gene            9820..10806
                     /locus_tag="PLT31_03115"
     CDS             9820..10806
                     /locus_tag="PLT31_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94159.1"
                     /translation="MPDFNLFRFQNQLNTKPLEEGLARDYENAGRSIGGLIGLGLKIK
                     GDKEAEEKNAALEKNKKDFLNFLDNYGNGLTDEAIMREGNRYGFPEIAAQFVEAKSGR
                     LSREESLRARTAAEKESRDTGKAIGESNRLTLQSNIQDLEDEINELTLKIKSRSKESQ
                     EARNLAAKRNALKKRANDMKKNYEIKFGPFESNLIDDDKIETDDSVYEEIESIVDRLK
                     DNDSFIKEFSGLSDTFQSLEKDDASKTLDKLKLFESKLEKNKSALKDRKDKAYKSIEN
                     LKNKTVKGGNVSNNRFILDDIAALHKVVYGKNLSRSEVVNTYKLNLVEGEDY"
     gene            10806..12791
                     /locus_tag="PLT31_03120"
     CDS             10806..12791
                     /locus_tag="PLT31_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94160.1"
                     /translation="MSKGIFYPIDFGDKEKKIVEKNYSKIIELDRLLRDGSIDESEAA
                     KNELLKMFETRFVSDIFEPVKYAEEIATRKRSDREEESRKSLKDVGDRETPLLTPEDI
                     IKAYKPIDEEGNLYVMAKAPNESDLAFISRQKAAFSNMGLPWNTEAKRLVSSVMQDAG
                     IKGARAKVVSDYGKSLGGFLGSFVVPRTKESVEKDILEGGEGKIKKGDLALDIGENLA
                     QTAAPFSRLFKGVKYSKAIPRAVGNASFAPVVSEIADALYYDEKTNKDRANPSLFDIV
                     TAAGLNTAADYKAIKRGRQAMREAGIPITGYSRNYLAQKELKRMTKENAIRNKEEAAK
                     NLKRIQELPNFISDAQTLKNPNKIRKAIQEKGYSIDELEASFPSVQAFDDWLNGRVVF
                     ENIEDIDNLTIPLLKKARADYQKASSEAAEVIMKSAEKKGEGVFGYFKPKKPARFSRV
                     KDDFVKEKDFEKGKEIVDNLLAAKKKNPFDKKTNNFFMGELKREGFTNKEIAGMMLDK
                     GYQPSVTEYTSGVFNQVIKEREKLKNAKPIKPKAIKKDKSFEALSENPEAYRVLGTGK
                     KVSAAEKAKYEQSLPKKMPSQFKEDITSPENRQNIFDLVRGYAIREASPFVSGSLTRD
                     IEPFFMDDIEEERLDKKKLDEKKSLEKKQLFKKLYGR"
     gene            12797..>13234
                     /locus_tag="PLT31_03125"
     CDS             12797..>13234
                     /locus_tag="PLT31_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94161.1"
                     /translation="MLLSKALKMSKSKAKRIRNTAWYSGNIEADGKIDKDTYYKSSYH
                     SNINPQKSKKGTCNYCLRCNGRIFKGSDLILANGEFGHHPNCKCSFTPVEKGVKVKNG
                     FVTNKDYGARKTLDARSMKALPISQLGMIARKRGFENKIADKGN"
BASE COUNT         4943 a         2019 c         2657 g         3515 t
ORIGIN      
        1 tttttattta tatttgaaaa agaggatttt gttttctccc tcctctgaaa ggctgcttag
       61 ttgattctaa gtggcctttt tttatacctt gttgtgtaca aaataactta atttatacgt
      121 acaaatgtta atttatgcgt atatttatac acaaattcat tagtttgtgt aataaaatgt
      181 gtatattata gtagtaaata cacatacaac agacagagac actataatga aagagcaaaa
      241 cagaaaaaat aaacttgatg agcttgaaaa agaaatcaac gctcttaaag aaagagttac
      301 aaagatagaa gcatctttcg aagtgaaaga taaagaagaa gaaaaaccta aaacttcgtt
      361 tatgtaaaat gctaactgat aaagacatta aagaaggtca aaagacaaga caagagatgc
      421 ttaacgcttt tagagcgagt agaaagatgg tgtcttggaa aagaagaggc gttgcgaaag
      481 agcttcctga aagaaaatta attaatctgc ggcgtgatta ttacaattgg ataaaagaaa
      541 attactttcc aattttgaaa cacccatctt ttttagacca gaacgacccc gattttctat
      601 taaacgcatg gcgttctttt tgcacgatgt cacttattcg atgcagcaat cactgtaatt
      661 ttttactttc tatctatcca gaaagtgcga agtattttat agagataggt aaaattaaaa
      721 aaatattgcc tcttcgtagc tccttcgttg atacatataa agagggtgat gattcttatt
      781 ctaaagagag agttaaggta gaaaaagatt ttgcagggtg gtttcaagag gcaaaagaac
      841 aaattaaaac actacttgca caagactgct atttttttgg caatgttaaa aacaattcgc
      901 cacaatactt catggagaaa ttcgtcgtcg ataaagaaaa taaagaagac aaagacaacg
      961 aatccaaaac agttcaagtt gattttaata taatttctag acaaagtaac gccaatgaaa
     1021 ataaaagtta gcgtagatat atccgagcaa caatcgcgat ttattctttc agaaaagaag
     1081 ttcacggcat ttgttggtgg gcgtggttca gggaaaacat ttattcttgg acatagagca
     1141 gcaggaagag ccgcaaatgg aaggaatcag ctagttgttg ggagaacata taaagagctt
     1201 aggcaaacaa tcattccaag cgtaaaaaat gctttagaat tatatagaat tccttttaag
     1261 gaaaataaaa atgacaacat aatcacgctt aaaaaaagta agattctttt tttatctgga
     1321 gagaatccag aagccattcg atcttataca gactatcacg atggatatat agatgaagcc
     1381 gcttggcaaa agcaaatggt catgaagaac ctgtctatgt gcttgcgtgg taaagatgtg
     1441 ttgaatcctt cagttagctt tacaacaaca ccaagaggcg gatcatggtt taatttgttt
     1501 gtaacgaaat caaatcaaga aaaattagag ctaataaagt gtaccacttt tgataatcct
     1561 tttttatctg aagattcttt agagcttttc aaagaagcat tatctggaga cgagaagcta
     1621 gctaggcaag aactatatgc tgatatgctt tcagagtctc caatcaatgc ggtcattcca
     1681 gacaacttaa tctttatgag cgatgtgcct ttagacttgc ctgtgtctat tggaatagat
     1741 gttgcaagag aaggagtaga taacactttt atagtcgtta gtaatgagaa aggcgttgtt
     1801 gaatcgaata agttttcaaa cttggatggc attaatttgt acttaaattt tgagaagata
     1861 gcaaagaaat ataaaacctt gtgttctgtg aacgttgata atacaggcgg ctgggcatct
     1921 tcatttcttg acattgtaaa aacaacagac tatagagaca aagtatttgg tataaacttt
     1981 gcgtcatcag caagtaacaa catatacgca aacaaacgag cggagatgct tttcaaatta
     2041 agagatctat gtgaaaagaa aactttctca cttaagaact gtcaagatgt tgtaagtgaa
     2101 ataaatgcaa caacatattt tttgaacggc tctggacaac gccaaataat tgacaaaaaa
     2161 ttaataaaaa aagatatcgg tcattctcct gacgagcttg acgctgtatc tttaagtgta
     2221 ttaaatggat ttaatttaca tacaaatgaa gcaaggtatt cacccttgcc acaacaaggt
     2281 tggtaattat gaacggaatt gtaaaagaat ttatagactt tgcaaataaa gcgaaggaca
     2341 aatttaaatc agaaatagat gagatgaaaa tcgaccgtga tattttctca tctacaaaag
     2401 tttgggatga gatagatgaa aaagtcagag gaaaaagtag ggacaagtct acagtaaacc
     2461 cgctccagaa gtacaagaat gctatatgta acagctttaa cagataccct tacgatgttg
     2521 aagtcgtagg acaagcacca gaaataatta aagaaaaatt aaagaaaata tctgttgact
     2581 ctggactaaa taacatcata atgcaatgcg tgtctgactc tgttattatg ggcaagggct
     2641 ttgctttcat tacaactaac ggaaatgaaa tacagataaa ttattcagac gacccatcac
     2701 aagtcattat agatagcgaa tcaaaagcaa tcgatgggag cgacatacag aagattgctt
     2761 ttgtagacaa gatgagctat gaaaagataa aacaaacctt tgcttctttc acgctaacag
     2821 aagatgaatt gaaggcatca aaaaatttag atgttggatg ttgggaatca ggaaagtata
     2881 gttacaacgt cattagttac tttacattaa atgagagtgg cgttactttg tatagaatca
     2941 taggcgatga agtcgttgag accattgttt acgaaggact aaagaagata ccagtagttc
     3001 ctttgtatgc aaatgaattt tggaaagaag gaagcaggca ttacaagggc attgtaaggg
     3061 atgtaaggga tttaattaaa ataataaact actcttactc cgctttaaaa gaaagacttg
     3121 cttgcccatt gatcccaaaa acaagggttt cttttgagtc ggtagaagga tacctagaag
     3181 actacacaca agctaacaaa tcaacagcag gcgttgagcg ttataaagaa tggtcggtag
     3241 atggtagaaa attaacggct ccaattacag aatatccaga attaaaaagt aatgacttat
     3301 tagtcgtaat tggaaatgca aaaagtgaga tttcagatat cattggcgtt ccgcaatctg
     3361 ggcttgcttt tgaaacagac tctgcacagg caacagcgac tgaagtttta cttcgatcac
     3421 aagctaacgt taataatgta tcccattatt atcaaaacgc aaaatcaagt ctaaaaaact
     3481 tgatgagcat ctgcttagat ctcttgtgtt atgtagaagg cgtagagaat acttttgaga
     3541 tcgcagttac taacggcccc gaaacttatg tcagaaaaga gatgctaagg cagcaactga
     3601 tggcaacaca atcgcttgtg cctgatgccg tgaagccttt gattatggca gaagttgtga
     3661 agactttaga aatagaaaat gcagaagcat tgagtaacgc cattcttttg actttacctg
     3721 aagagctaag gccagtgaat aaaacaccgc aggctttggc tttagaatta gtacaaagta
     3781 agcaacagat agaacaactc cagcaagcat tacagcaact tagcgaacaa aataaacagt
     3841 tgcaagagac tataaatact gatgtaatta atagtcagaa ccaattgctg atggttcgag
     3901 tacagaatga aagctcttta aaaacaaagc taatagagat gcaacaacgg aataaagaat
     3961 tcgaactaaa ctatcaatta gaaattgcga aagtagatgc atcacaacga gaaaagctga
     4021 tggaacaagc taatgagcag gctaagatac aaaatacatt aaggcaaacg gctttaaaag
     4081 aaattgagct agcagaaaaa atgaggttag aagaggaaca aaagagaagc gagctgttag
     4141 cacttgtact aaataaagat taatttgtat taaaattagt ttatatgtaa cacttgtaat
     4201 tttatttaat tatatttata aaaacaaagg acagagatat ggctgaagag caagatataa
     4261 tcaataagta tcgtggcatt tcgtcaaacg atactgaaca aaaaacagaa gagcctgtag
     4321 aagaacagac tcaaacaccg acagaagaaa ccgagactcc agaaaaaaaa ccagtcgaag
     4381 gtgaaagcgt cgagacacca gaagaaaaga attctgaggg tgaaaaagaa tttaaaaaca
     4441 atagttctcc atacgtcgag cggattataa aagatcgatt agcacgacaa gcaaaaaagc
     4501 atcagagaga actagaagcc ttgcgagctg agctgcaaag cctcaagaaa aaagaggaag
     4561 atccagagtt cacaagagat gactttattg atgaagaaga gtttgagcga tttaaagcag
     4621 acaaattaaa gaagtcaata aagacagatg taatgaaaga atttgaatcc tatcaaagag
     4681 agagagaagc tgaaagagca cagcaggaaa aagtaaacgc aactatcgca aactttctta
     4741 aaactcctga agagttacaa gaatggaaga gcaggctaga agattttgaa gaagactatt
     4801 ctgattttct agagagtgaa caaggacaag agatgtcttc gttcatgatt aacagtagcg
     4861 tattcccagt gatgtttgat ttgattgcta gaaaccctag cgttgtagac aaactaagct
     4921 cactgagtac aaaagaagtt tacttcaatt taaagcagct cgaagacgca attctgaaaa
     4981 aaataactgg gataaaagaa gcccaacaac aagaaaataa aaatgcagaa gaacaaaaac
     5041 caaaacgttc tctgcctaat tctggaaagt tcggaggatc ttcatcaagc acatcatcaa
     5101 ggctagatcc aaacagcaaa gactttgatg ctaaagaata cttgaaaaga aaatatccga
     5161 atcagtacta aaaaggaaaa aaattatggc aaacttatct acaaacatta ccacagcaca
     5221 acttgacatt ctgtctgttg ctgttgaaaa atatgcccct atccttgaag acgttcgatc
     5281 ttcacagaag ggactaaaag ggcgtactgg cggcctcctt cgtgttgtca ttccagattc
     5341 tggatccgta gtgattacag aaaatgggct gcgtgatatt tcagctgtga ctttagataa
     5401 tgcagaattt tcaaaagact taagaatttc ttctgctaac actgctttta gtgctacggc
     5461 attagagaga gtgacaaacg ttgatgattt cgataaagag atcgtgcaac ctcgtgccgt
     5521 gaactacggc gaaacagtca acgaaaccat cattgacaaa gcatacactg ttgctggaat
     5581 cgcacaaact gcggcacttg caacacttga atttgatgca ttagcaacaa ctgctggtaa
     5641 gcttcgtgaa aatcgagcta ctaatttggt aggctatatg tctcctactg tcgctgcaaa
     5701 gctaggttct aaaggggcta acggctcgtt ccttcctcca gcaattttgg aaccaatgta
     5761 taaagattgc caaattgggc gtttcgcaaa cgtccaatgg aaagaatcaa agatgccnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncac gccaatgtca gcgtcatgcc
     5941 agtagctact aacagaccaa ctcctgttaa ctgcggtgca gcaaaaacat attctgttgt
     6001 tttcgttttc gaaaaaggga acattgagta tgatgctgtc gagctaaaca ccgctggctt
     6061 tgaatccgtt tctgtttctg gaatcgattc caaaatcaaa acaactgcat tagttagtgg
     6121 cgacatcaac acgctaacag caaagtatcg aattgattcg gcctttgtaa ctggcggtat
     6181 tgatgaccgt agagccgctc tattgtttgt agaaatctga taattaagtt gtacacacaa
     6241 gcttaattta tctatattat ttattaaccc tacctgtctg ggtaaaagac agtgcgaaga
     6301 gtttttttcg tgctgtcttt tttttattag gattaaaatg acaataagag atatcattac
     6361 attaatagcg gcagacatta actacacgag cggaaataat tccgtcgggt tagaagaagc
     6421 aaaccgaatt ctttcattga tgaatagatg cattaacatc tataacacgc aagggctact
     6481 gtcattcaat tatcacagcg aaacaccaca aaaaataaat ggcgattact ttgtaagtaa
     6541 cggaatagat gttgctgcgt tgtatgtatt atgcaattca agcaagctac gcataaagca
     6601 agtacaacta acatctcttt atgagcttac taatagtggt gtgatgccat cactattttc
     6661 tataaaaaga aatatagatg taaatggtgt tcgaatgatt caacttttct ttgatacaaa
     6721 aaatgtgtct tatgaattag aagccgtcat aaaagaagac ttgcctgcat ttaatttgaa
     6781 cgacgaattt acgctgcccc cagaatatca aaacctttta attagtgacg ttcaattacg
     6841 cttacttgtg aatgatgata tttctccaag ctcgttactt tatatagaaa agaaaagaga
     6901 atttgaagaa gtaaaaaaat taattaagga agcaaacttc aaaaattatg attttggaga
     6961 atatgcaata agcaagtttg ataagttcaa tgcaggtttg ttcctatgaa aaagattctt
     7021 aattcattta ctggtggatc gtcaaagtac aaagatttag actttgtttc tcatgaagaa
     7081 aactttaata tgtttccaga aacgctggaa tctaatgagc attacacgaa taaagttcta
     7141 aaaagtctta ctggatcaag aacgatatta tcacaattag gtggattttg taggggacta
     7201 tacattgcat caacaagtcc attgacaagc tataacgctg gaaccccttt gctatatggc
     7261 gtttatgggt cgaaggttta tagaatatat aataattttt cttatgatta tataggagat
     7321 gttgcagata atagcgagcc tgtatctttt gcggagacaa gcggtgttcc tgcacatctt
     7381 tgtatctgct catcttttaa tatttacact attaatttag agactgaaag ctcgttagta
     7441 tctgttgatg taatggagtt gccaaaaaag gctggagagc tagtaagcat aaggccgaca
     7501 atgattacgg ctttgaatta tagaatcatt tgtaacgaca aagatagtga ttacttttat
     7561 tattcagagc ttggaaagcc gaatggcatt aataacaatt acgctttcta caagtatatg
     7621 acaagatata ctttcatgaa aaaggacgga actttagtaa cagcagacga taatcaatac
     7681 tacccacctt cagaaggttc atatgttgaa gggactttag tcacagaaga tgtatggatg
     7741 ggatcgctta actacataaa ggccgagttc agaagcgaca acatagtagc tataaaggca
     7801 atggatgatt atctatttgt tatcggttat agttcttatc aagtatatag gtggcaagat
     7861 aacatcaaca ttccttttat aacatctaca aaaaatagtt caataggatg taaggcacca
     7921 tatagtgttt catcaattaa taacaaatta atttttttag gtgcatcatc agtaggtaca
     7981 aatgcaattt gggtaagtga tgggcaggga atagaaaaga tttcttcagc atggatagaa
     8041 gagcaaatag aaagctttac aagaacagat gatgctttct cattttgtta tgttgatggg
     8101 aaacatacat tttatgttat atcattccct tctgctaata gaacttattg ttatgacttt
     8161 gatgaaaaag aatggcacac aagagcgaca agagacatca acaacgaaca aaaatgctgg
     8221 tttcctgcat ttgctgtaaa gtattctgac aaaataataa tgggtgcttt caatgaagac
     8281 aaattaattt atctagataa aaataaatat acagattaca acgacaaagt aatagaacgc
     8341 tcaagaacaa ctgggataat aataaacaat tttaaaaagg tgatcattca ttctttagaa
     8401 ctaattatta gttctggaaa aacgaatgta gaaaaagagt acgacgatca aatgaatggg
     8461 gcaacgcctg aagggtacaa tcctaatgta atgcttataa ctagcgcaga tgggggctat
     8521 acatggggtg gtgaaaagtg ggcgaaggct gggcgtatag gagaatacaa ctcaagatgc
     8581 atttttagaa atttaggagg cttacgaaga attgcgttca aagtaacgtt tactgaccca
     8641 gccccattta atatttctaa agctattatt gattatacag agtgcggtag ataaatgata
     8701 acaagcaacg taataaatcc atattcttca gaaacaaaag acttacttcc gttagtgcta
     8761 ataaaaaatg gtatggtcgg ttttcttcaa tcggatggat taattaattt atctacaatt
     8821 aaaaagctaa atcttccaaa tgctgtatat gactacacat tagactttaa tttttatcaa
     8881 aaaataaacg gtgttaattt gtaccaaaga acaaacaaaa tttatatttg taaggaacag
     8941 acatcattag atatttcatt agtcggcctt gcgtttatcc aagcaaaatt aattttaaaa
     9001 caatgaggaa aaaaatgtca ataggtagtt ccattaacag tgccatttca ggtgttacag
     9061 atgcattcgg gcttactaat gtaaagggta aaaaaaaggc gtatgataat gcagctagta
     9121 tgttacaaga tactcttgct aaatcaggaa aaacatatag ccaaattttg gaatccataa
     9181 aaggaactgg ccgcagtctg caagatcagc taggtggatc ggcttcagta agtgattgga
     9241 ttaatagtat taaagaagct gggaataaag actattcagt tgactcgtca aaagtaagcg
     9301 attttgattg ggataaaaca gtcagtgatt acttagaccc aaacgcttca tatatgatag
     9361 atcaagcgac acaagccgca caaaatacat tagctgggca aggtgggtta ttcagtggtg
     9421 gggcagggca gcaactgcaa gccgttgcaa gtgataaagc tagggaacta tacggcgatg
     9481 ctcaagaaca aatgaacaaa gaaaaatcat ttgattacaa caagttgctt gatgagttga
     9541 atattgaagc tggaaattta ggccgtgaac aaagtcaaga catggcttat agttcaaact
     9601 tgggaaatgt agcgaacgct tatcaaacat cagtgagaga tacacaagag ggcgtaaaca
     9661 atgctctatt atctcaatta caaaatgact catctattca gcaagcacta gcaaatttgg
     9721 gtatttcaga agcatctgca ccaacggcat tgggttcgat attcggtgat gttcttgggt
     9781 ttgcaagtgc gtttattccg caaaaaggag tgaaagaaaa tgcctgattt taatttattc
     9841 agattccaaa atcaattaaa cacaaagcct ttagaagaag gtcttgcgag agattatgaa
     9901 aatgcaggcc gctctattgg tggcttgatt ggattaggat taaagataaa aggggataaa
     9961 gaagcggaag aaaaaaacgc tgccctcgaa aaaaataaaa aagacttttt aaactttctt
    10021 gacaattacg gtaacggctt aacagatgaa gctattatga gagaagggaa tagatacgga
    10081 ttcccagaaa tcgccgctca atttgtagag gcaaagtcag gacgattaag cagagaagaa
    10141 tcactaagag caagaacagc agcagaaaaa gaaagccgag acacagggaa agctatagga
    10201 gagagcaatc gcttaacatt gcaatcaaat atccaagacc ttgaagacga aataaacgaa
    10261 ttgacattaa agataaagtc acgctctaaa gaatcgcaag aggctagaaa cctagcggct
    10321 aaaaggaatg ctcttaaaaa aagagcaaac gatatgaaga aaaattatga aataaaattt
    10381 ggtccttttg aatctaattt aattgacgat gataaaatag aaacagatga ttctgtatac
    10441 gaagaaatcg aaagtatagt agatagatta aaagataacg atagttttat taaagaattt
    10501 tctgggttat cagatacttt ccaatcacta gaaaaagatg acgcatcaaa aactttagat
    10561 aaattaaagt tgtttgaaag taaattagaa aaaaacaaga gtgcgttaaa agatagaaag
    10621 gataaggctt ataaatcaat tgaaaactta aaaaacaaaa ctgtaaaagg tggaaacgta
    10681 agcaataacc gatttattct tgatgacatt gcagccttgc ataaagttgt gtacggtaaa
    10741 aatctatcaa gaagcgaggt agtaaacacg tacaaactaa acttggttga aggagaggat
    10801 tattaatgtc aaaaggtata ttttatccaa tcgacttcgg agacaaagag aaaaaaatag
    10861 ttgaaaaaaa ctattctaaa atcatagagc ttgataggct tctgcgtgat ggatctattg
    10921 acgagagtga agccgcaaaa aatgagttat tgaaaatgtt tgaaactcgt tttgtgagtg
    10981 acattttcga gccagttaaa tatgctgaag agatagcgac aagaaaaagg agtgatagag
    11041 aggaagaaag ccgcaagtca ttaaaagatg ttggtgatag agaaactcca ttgttgacac
    11101 cagaggacat tataaaagca tataagccga ttgatgaaga aggcaatttg tatgtaatgg
    11161 caaaagcacc taatgaaagc gatttagctt ttatctcaag acaaaaggcc gcatttagca
    11221 acatggggtt accttggaat acagaagcca aaaggcttgt atcttctgtt atgcaagatg
    11281 ctggaataaa aggggcaaga gctaaagtag ttagtgatta cggaaaaagt ttgggtgggt
    11341 ttttaggttc ttttgttgtt ccgaggacaa aagaaagcgt agaaaaagac attttagaag
    11401 gcggtgaagg gaaaataaaa aaaggagacc tggctcttga tattggtgaa aacttagcac
    11461 aaacggcagc accgttttca agactgttta aaggagtgaa atattcaaaa gctattccaa
    11521 gggctgttgg aaacgcatct tttgcccctg ttgtttccga aatagcagat gctttgtatt
    11581 acgatgaaaa aacaaataaa gaccgtgcta atccatctct ttttgatatc gtcacggcgg
    11641 caggattaaa tacagccgct gattacaagg caataaaaag aggaaggcaa gctatgagag
    11701 aagctggaat tcctattaca ggctattcaa ggaattattt agcacaaaaa gaattaaaaa
    11761 gaatgacaaa agaaaatgcg attagaaata aagaagaggc cgctaagaat cttaaaagga
    11821 ttcaagagct tccaaacttt atttcagatg cacagacgtt aaaaaaccca aacaaaataa
    11881 gaaaagcaat tcaagaaaaa ggctattcta tagatgagtt ggaagcctct ttcccaagtg
    11941 tgcaagcttt tgacgattgg ttaaatgggc gtgtagtctt tgagaatatt gaagatatag
    12001 ataacttaac gattccactt ttaaagaagg caagagccga ctatcaaaaa gcatcttcag
    12061 aagccgccga ggttattatg aagagtgctg aaaaaaaagg agagggggta ttcggttatt
    12121 ttaagcccaa aaaacctgca agattttcta gagtaaaaga tgattttgta aaagaaaaag
    12181 attttgaaaa aggtaaagaa attgtagaca accttttagc agcaaaaaag aaaaacccat
    12241 ttgacaaaaa aacaaataat ttttttatgg gcgagttaaa aagagaaggt tttacaaaca
    12301 aagagattgc tggcatgatg cttgataaag gctatcagcc aagtgtgacc gaatatacca
    12361 gcggcgtttt taatcaagta attaaagagc gagagaaatt aaaaaacgca aagccaatca
    12421 agccaaaagc aataaagaaa gacaaatctt ttgaggcatt gtcagaaaat ccagaagcat
    12481 atcgagtttt gggaacagga aaaaaagtta gtgcggcgga gaaagcgaag tatgaacaaa
    12541 gcttgccgaa aaaaatgccg tcacaattta aagaagatat tacaagtcca gaaaatagac
    12601 aaaatatttt tgacttagtt aggggctacg ctattagaga ggcttcgcca tttgtttctg
    12661 gctcactgac tagagatata gagccattct ttatggatga tattgaggaa gaacgactag
    12721 acaaaaaaaa gctagatgaa aagaaatctt tagaaaaaaa acaattgttt aagaaattgt
    12781 atggacgtta gtttatatgt tattatctaa agcactaaaa atgagtaagt ctaaggcgaa
    12841 gcggataagg aataccgctt ggtattctgg aaacattgaa gccgacggaa agatagacaa
    12901 agacacttat tacaaaagtt cttaccactc aaacattaat cctcaaaaat caaaaaaggg
    12961 aacttgtaac tattgtttaa gatgtaatgg ccgtattttt aaaggatccg atttaatttt
    13021 ggcgaatgga gaattcggtc atcatccaaa ttgtaagtgt tcttttaccc cagtggaaaa
    13081 gggcgtaaag gtgaaaaatg gatttgtgac aaataaagat tacggtgcaa gaaaaacgct
    13141 agacgcaaga agcatgaagg ctttacctat ttcgcagctt ggaatgatcg caagaaaaag
    13201 aggctttgaa aacaagattg cagataaagg aaac
//
LOCUS       DAOOBB010000039        13152 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_6088_length_13152_cov_5.278766, whole genome shotgun sequence.
ACCESSION   DAOOBB010000039 DAOOBB010000000
VERSION     DAOOBB010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 13152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13152
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6088_length_13152_cov_5.278766"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1230
                     /gene="polA"
                     /locus_tag="PLT31_03130"
     CDS             <1..1230
                     /gene="polA"
                     /locus_tag="PLT31_03130"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPW94162.1"
                     /translation="EKFFLELEMPCLKVLFEMEEKGVSVDSEELKRQSAEMELRILDL
                     EKEIYALAEGVEFNIASPKQLSEILFDHLGLPVIKKTQTGRSTDSVVLEELSVVAPHP
                     IVFAIMEYREIKKLQSTYLSVLPELVNPETKRIHTSFIQWGTATGRLSSREPNLQNIP
                     IRTEAGKKIRAAFVPAKKEDVILAVDYSQIELRMLAHLSGDEKLIAAYNHGADIHTQT
                     AAAIFNVPLAEVTSDHRRDAKIVNFGVLYGMTAFRLARDLKISRTRAKEFIDDYFALY
                     QGVQSFIDQTVAFAHEKGYVETLTGRRRYIPGIDSHDRTECQMAERMAVNTPVQGSAA
                     DLIKIAMIRIAERIEKEKLPLKMVLQVHDELVFECPKSEVEALSMMVKEEMESAMKLS
                     VPLIASVGFGSNWLSAH"
     gene            1275..2360
                     /locus_tag="PLT31_03135"
     CDS             1275..2360
                     /locus_tag="PLT31_03135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPW94163.1"
                     /translation="MKKNWIITLGIFLLVACASEEKVDSSKKGSRKGRSQARMISVEG
                     YLAEPGQTISSSKLAGNLSAYDEVELKTEMAGKLVALKVKDGAYVKKGSLIAKINDAE
                     LQAQLKQANASLELAKQKETRTKTLFEKQSATQAELETVVAARASAEASVALILAQIR
                     KTEVIAPFSGTLGILKVSAGEWVTTGTSIATLANVQKLKVSFSLPQRYATSVSKGTKV
                     KINDSERGISAEATVRVLEPILSQNNRSRIIQAEINNKDNLFIAGSFVEVTVPIEAGS
                     EKEVIHVPAEAFTLDDKGPYLFVAEAGKAVKRYVTTGLRTPISISVLDGLNKGDTIIV
                     SGMMSVREGISIQIKEWRRKLNYGVKK"
     gene            2357..5407
                     /locus_tag="PLT31_03140"
     CDS             2357..5407
                     /locus_tag="PLT31_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPW94164.1"
                     /translation="MSLAQVSIRRPVLMTVLSIGVVLLGFFGFSNLGIREYPSVDSPV
                     ITVTTSYSGANAAVVEAEVTEVLEEAINSASGIKTLTSTSQDGRSRIKVEFDVGFDME
                     TAANDVRDRVSRVQRRLPDAADAPTISKSDADAQPILMLSLLSDKRDAMEISEIARNQ
                     VKERLQTITGVSEVAIWGEKRPVVRLWLDPIKMQSLQVTAADISTALAKENLELPAGK
                     IEGEVVDLSIRTLGRLQKPSDFSAIAVKKTASGVIRLADIAEIHYEPKDPRTGWKRNG
                     KNIVSIALIPQPGSNHVDIADAYYKRLEEIKRDLPEDVQMIRGMDATKNIRASIEEVI
                     GTLVIAFILVIFMIFAFLREVRTTFIPMIVIPIAIIGSFFVLYLFDFSINVLTLLAMV
                     LAIGLVVDDAIVIVENIYHKIENKMAPKQAAVAGTNEIFFAVIATSIVLMSVFVPMFS
                     LGGTTGLLFREFVAVMIGTVFISTVIALTISPMLCSKFLRHQKKGRLFIITEPFFISL
                     NQIYARLLGAFLKVRFLMFPLLIILLGLALFFFKALPQEMAPLEDRGFFMARVSLPEG
                     LGIAKTRAITEEIVDDMTSRIDTLDYEEITAGANGNSATIRVVLNENIKERRSQSEIA
                     RQVQAMGQEYPDVRLMVIEPQGLSSQRGGMPVDFVLQAPSIDVLRELVPVFLEEAQKD
                     PTFSVVNSDLKFTKPELHIEILRDKARESGVYVADIASAVELALGDQTYGDFYMNGRQ
                     YDIIGAVGLQYRNSPDLIRTLSVKNNNGEILSLDNFVTLTERAAAPSLPRYNRFSSAT
                     ISAGLVQGKTVGEGVEAMRAIASRILKDYPTVSTDLAGTSKEYEESSSGLLLTFLLAL
                     AFVFLVLAGQFESFRSPFIILFTVPLALVGALSALYAVGITMNIFSEIALILLIGLVT
                     KNGILIVEFANQIAESTGCSRLEAARLAAERRFRPILMTSSSTILGSVPLIMTGTPSR
                     VSMGVAIVGGLTFATLMSLFVVPAAYSFFAGLVEKKSLDASKMT"
     gene            complement(5529..5795)
                     /locus_tag="PLT31_03145"
     CDS             complement(5529..5795)
                     /locus_tag="PLT31_03145"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HPW94165.1"
                     /translation="MISQLIFGKNGLLGQHRLYNHIQSLEIEIDSLKVELQKQQDENK
                     RLKTDSFYLEYLVRTRYGMSKKDELPIQFIDIKKEKPATLPTDE"
     gene            complement(5846..6751)
                     /locus_tag="PLT31_03150"
     CDS             complement(5846..6751)
                     /locus_tag="PLT31_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990814 - DNA/DNA annealing activity
                     [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPW94166.1"
                     /translation="MKNKDSNSRQIFPSDFPESLQKSIHCPKSLFIKGTLPDKNTVGV
                     ALVGTRRPSVSAPLLCDLLVKSLRGTNAVVISGLAQGIDSLCHEAALKYGIPTIAVLG
                     QGLNSEIGGSRKILAQKIIEQGGALVSTFENDSPAFKWQFPLRNGIIVGLSKTTVVIE
                     SKTKGGALITADLCLKEKRTLLAIPGDFNRETASGTNALLRRGLAKALFSPEDLGLYC
                     GLTQKNDPSKETLLKADISLSEDATKILEKVGGYTYTIEEVQELFPFKRHVLLSILTE
                     LEIAGLVYSKDGFRYHFSLMEFSSC"
     gene            6750..7535
                     /gene="mazG"
                     /locus_tag="PLT31_03155"
     CDS             6750..7535
                     /gene="mazG"
                     /locus_tag="PLT31_03155"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HPW94167.1"
                     /translation="MAYSFNDLIDIMKRLRAPDGCPWDREQDINSLLPYLIEESCEYI
                     DAAQANDKVHMCEELGDVLLQVVFHAQVASEQGDFSIDEVVESICEKMIRRHPHVFGD
                     ATAEDANAVLREWERIKASEKNNIGMAEKSAMDKVSKSLPPLARAQELQRRAAKVGFD
                     WSREAPVFEKVLEEITEFRKEIQAENKNKEKIEEEFGDILFSLVNWARHLGFNASTAL
                     MRSNAKFDRRFRAVEKLAGDQDMKSMGLEKLDALWDEVKKEEH"
     gene            complement(7575..7790)
                     /locus_tag="PLT31_03160"
     CDS             complement(7575..7790)
                     /locus_tag="PLT31_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94168.1"
                     /translation="MIKIILLIIVSIVGLGLSILYLRKNLVRIAEKNKSIDSTGKKVL
                     NYPLTILWYGYLIAFFIGLTVNNLILN"
     gene            8107..8556
                     /gene="rimP"
                     /locus_tag="PLT31_03165"
     CDS             8107..8556
                     /gene="rimP"
                     /locus_tag="PLT31_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HPW94169.1"
                     /translation="MDNREKLDSLIADACQSVGVELVELDQFQAGKRKVLRLYVDKPE
                     GVTIGDCTEVSRQLSAALDLDEEIIAGAYTLEVSSPGLDRPLKSTADFQRNMGRLLRI
                     TRSTGKPVTGILQSVDEQNIMLDLKGHAGEFQVPRSEILSAKVDIQF"
     gene            8569..9831
                     /gene="nusA"
                     /locus_tag="PLT31_03170"
     CDS             8569..9831
                     /gene="nusA"
                     /locus_tag="PLT31_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPW94170.1"
                     /translation="MKNEPKINLVEALKSLVQDKNVEDSIVTGALKDALISAARKYLQ
                     INKKIDVDIDTETDEIRVFLRVEVVDDYPDYDTEMSAEEVEELDEGYMLVQEAQEFNE
                     DAQPGDLLEMEVPVSAFGRQAIQTAKQFLIQRIRTAERDKIYETYRSRIGTMINGEVV
                     RIENKNLIVSIGRQTEAVLPAKEQIAKERFHQGSSVKAVIADVTDSTKGGAQVILSRA
                     NGDFLKELFRQEVPEIYEGTVEVKGVSRDPGFRAKIAVYSRDERIDPVGSCVGMKGAR
                     VQAIVRELANERIDIVHWNPDFETYVRRSLAPANIVKFVEVSGTRRIVLVVADEDLAQ
                     AIGRNGQNVRLASQLVGRDLDVFGEKEWSEKDEETKEKILTPRANEYRQMNQMNDKKM
                     QLDELFTKENTEEQPIEEDGVSMEESEA"
     gene            9860..12946
                     /gene="infB"
                     /locus_tag="PLT31_03175"
     CDS             9860..12946
                     /gene="infB"
                     /locus_tag="PLT31_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPW94171.1"
                     /translation="MSKIEEIRPADWAKSKGVTSEIVVKLLKEAGVDVRSHMTKIPVS
                     EYAPIEAQVESERAKLDSRKQKKSSSTSDADNSSTSSAPKRKVGSVSTIKGGAVKVSL
                     KRAKPAAPKPASETKALAAPQKPAIAQPEVVKSVESKKENVAVSTAAEVSKPVEQKPV
                     QQKPVEQKSAEHKSVEQKSAEHKPAVAPRTTPQASLQPKKEVAPKEIPATSKEEDSKP
                     DLKVAAMKAQVFKPDAAILARIEKSRQQQANTQHRRPNGPRSGGNNSQGYTGSFGKSP
                     AGGGVNSNNRRPQGQGGNAGGGYRPGGNAGGGNRPYTPGGQNRSGGFSSGSMKEAFGA
                     SPSGANLVASTTTAAKDGSKSGNRRNDKKRGTSLGNKQERQKEQQDVVRQNVSRVMAS
                     LSKHPIKKVYRKELSESDGSVERQILKTSDFITVGELAGLMEQMPARVIAKCMEMGMM
                     VTINARLDFESIQVLADEFGFEAQLMEEYEEEVLGVEEEQTENLLPRHPVVTVMGHVD
                     HGKTSLLDWIRKANVVSGESGGITQHIGAYEVSTKAGKVTFLDTPGHEAFSAMRARGS
                     QVTDVIVLVVAADSMVMPQTVESIELAHREKVPLVVAITKIDLPTANPDKIRTQLAER
                     GVEVEQWGGTVSCIEVSARSGKGMDLLLETLALETEVLELKANPDTRARGAVVESKLD
                     AGKGSMATILIQNGTLHIGDPFVCGLYSGRVRAMFDERGGIRKEAGPSTPCQVLGFDG
                     TPQAGDDLIVVEDEKTAREIASKRRMAARERDLRARKTISLENVYEGAKDGKISELNL
                     IVKADVGGSAEALAASLEKLSNSEVKIHIIRKGVGAITDSDILLATTSQAIVIAFHLM
                     PSLSIREMAEKEGIEIKTYRVIYDCIEDIKNAVEGLLKPTTREEIVGEADIREVFKIP
                     KVGLIAGCMVTSGEVNRDSRVHVYRNGVELGLTEVQSLKRHKDDVKSVARGFDCGIGL
                     KGYDNIEVGDTLMFFKDVKVARTLADVARDEKAAAEKSAADKAASEKKQNDKGETK"
     gene            12943..>13152
                     /locus_tag="PLT31_03180"
     CDS             12943..>13152
                     /locus_tag="PLT31_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HPW94172.1"
                     /translation="MSRRTDRLGELFRIEISKLLQKGLKDPRVGFATINRVDITEDLS
                     FAKVLVSVMGSDKEKRDTIIGLRSSA"
BASE COUNT         4044 a         2267 c         2913 g         3928 t
ORIGIN      
        1 gaaaagttct ttttagaatt agaaatgcct tgtttaaaag tcctttttga aatggaagaa
       61 aagggtgttt ctgtggatag tgaagaatta aaaagacaat ctgcagaaat ggaattgcga
      121 attttagatc ttgaaaaaga aatctatgct cttgctgaag gcgttgagtt taatattgcc
      181 tctccaaaac agctttcgga gatattattt gatcacttag ggcttcctgt gattaaaaaa
      241 actcaaacag ggcgtagtac agatagcgtg gttttagaag aactaagtgt agtagccccg
      301 catcctattg tttttgcgat catggaatat cgagaaatta agaaactgca aagtacttat
      361 ctctcggttt taccagaact tgtgaaccca gaaactaagc gtattcacac gagctttatt
      421 cagtggggta cagcgactgg tcgcctttca agccgtgagc ctaatttaca gaatattcct
      481 atacgtacag aagcagggaa aaaaattaga gcggcttttg ttccagcaaa aaaagaggat
      541 gtgattcttg ctgtggatta ctcccaaata gaacttcgaa tgcttgccca tttaagtgga
      601 gatgaaaaat taatagctgc ttataatcat ggcgcagata ttcatacaca aaccgctgcc
      661 gctattttta atgtcccttt agctgaagtt acgtcagatc atcgtcgaga tgcaaaaatt
      721 gtgaattttg gtgttcttta tgggatgaca gcttttaggc ttgctcgaga tttgaaaatt
      781 tcaagaacta gagcgaaaga atttattgat gattattttg ccttatacca aggagtccaa
      841 agcttcatcg atcaaacagt cgcatttgca catgaaaaag gctatgtaga aacgctaaca
      901 gggcgtcgca ggtatattcc tggaatcgat tctcatgatc gaacggagtg tcagatggca
      961 gaacgaatgg ctgtaaatac tccagttcaa ggaagtgccg cagatttgat taaaatcgcc
     1021 atgattcgta ttgctgaacg aatagagaaa gaaaaacttc ctttgaaaat ggtcttacaa
     1081 gtacacgatg aattagtctt tgagtgtcca aaatcagaag tagaagcatt atctatgatg
     1141 gtgaaagaag aaatggagtc tgcaatgaag ctttcagtac ctctcattgc atcggttggt
     1201 tttggttcta attggttatc agcacattaa ttattgttgt tttaataaat agaggttttg
     1261 tagtttaggt tgatatgaaa aaaaattgga ttattacttt aggtattttt ttactggtag
     1321 cttgtgcctc tgaagaaaag gtggattcat cgaaaaaggg ttctagaaaa ggacgctcac
     1381 aagctcgaat gattagtgta gagggctatt tagctgaacc aggacaaacc atctcctcct
     1441 caaaactggc tggcaactta tctgcttatg atgaagtaga attaaaaaca gaaatggcag
     1501 ggaaattagt ggcattgaaa gttaaagatg gagcttatgt aaaaaaagga tctttgattg
     1561 ctaaaatcaa tgatgctgaa ttacaggctc aactaaaaca ggcaaatgct tctttagaac
     1621 ttgcaaaaca aaaagaaaca cgtacgaaga cattatttga aaaacagagc gctactcagg
     1681 ctgaattaga aacggtagtg gcggctcgtg cttctgcaga agcttctgtg gctttaattt
     1741 tagctcaaat tagaaagacg gaagtcatcg ctcctttttc tggtacttta ggaattttga
     1801 aagtctctgc aggggaatgg gttacaactg gaacaagtat tgccacatta gctaatgttc
     1861 aaaagttgaa agtttctttt tctttgccac agcggtatgc aacaagtgtg tctaagggaa
     1921 caaaagtaaa aataaacgat tctgaacgag gcatatctgc cgaagcgact gttcgtgttt
     1981 tagaaccaat cctttctcaa aataacagaa gtcgtatcat tcaagcagaa atcaataata
     2041 aggataattt gttcatcgcg ggttcgtttg tagaagtgac tgttccaata gaagctggct
     2101 cggaaaaaga ggtgattcat gtccctgcag aagcttttac tctagatgat aaaggcccat
     2161 atctttttgt ggctgaagca ggaaaagcgg tcaaacgcta tgtaactact gggctaagaa
     2221 cacctatttc aattagtgtt ttagacggct taaataaagg cgatacaatt attgtttctg
     2281 gcatgatgtc tgtacgtgaa ggcatttcta ttcagattaa agaatggcgt agaaagttga
     2341 attatggggt gaaaaagtga gtctcgctca ggtatcgatt cgtcgccctg tattaatgac
     2401 tgtattgtct attggtgtcg ttttacttgg tttttttgga ttttctaatc taggtattcg
     2461 agaataccca agcgtggatt ctcctgtgat tacagtgacc acgtcttact ctggagcgaa
     2521 tgctgccgtt gttgaagcgg aagtcacgga agttttagaa gaagcgatta atagtgcttc
     2581 tggaattaaa acactcactt ctacaagcca agatggccgc tctagaatta aagtggaatt
     2641 tgatgtgggc tttgatatgg agaccgcggc aaacgatgtt cgtgaccgtg tgagtcgtgt
     2701 tcaaagacgt ttgcccgatg ctgctgacgc gccgacgatt tcaaaatcag acgcggatgc
     2761 tcaaccgatt ttaatgctca gtttactttc agataaacgc gatgcgatgg aaatttctga
     2821 aatagcaaga aaccaagtca aggaaaggtt acaaacaatt actggggttt cagaagtggc
     2881 catttggggt gaaaaacgcc ctgttgttcg tctatggctt gatcctatta aaatgcaatc
     2941 tcttcaagtg actgctgcag atatttcaac ggctttggcc aaagaaaatt tggagctgcc
     3001 agcaggcaaa atagaaggcg aagtagtaga tctttccatt cgtactttag gccgtttgca
     3061 aaagccttct gatttttcag ccattgcagt gaagaaaacc gcatcaggag tgattcgttt
     3121 agctgacatt gctgaaattc attatgaacc aaaagatcca aggactggtt ggaaacgaaa
     3181 cggaaaaaat atagtctcca ttgcattgat tccacaacct ggttcaaacc atgttgatat
     3241 tgcagatgct tattataaac gtctagaaga aattaaaaga gacctccctg aagacgtaca
     3301 gatgattcga ggtatggatg cgactaaaaa tattcgagcc tccatcgaag aagttatagg
     3361 cactcttgtg attgctttta ttctcgttat atttatgatt tttgcctttt tacgagaggt
     3421 gagaactact tttattccga tgattgtgat acctatagca attataggtt cgttctttgt
     3481 tctttatcta tttgattttt cgattaacgt attgacttta cttgccatgg ttttagcgat
     3541 tggtcttgtc gttgatgacg ctattgttat tgttgaaaat atttatcata aaattgaaaa
     3601 taaaatggct cctaagcaag cagctgttgc aggcaccaat gaaatctttt ttgctgtgat
     3661 tgccacatct atcgttttaa tgtctgtttt tgttccgatg ttttcacttg gtggaaccac
     3721 aggattactt ttccgagaat ttgtagcggt gatgattggt actgttttta tatccacagt
     3781 gatagcttta accatctctc ccatgctttg ctccaaattt ttaaggcacc agaaaaaagg
     3841 gcgcttgttt ataatcacgg aaccattttt tattagctta aatcagattt atgctcgtct
     3901 attgggggcg tttcttaaag ttcgcttttt aatgttccca ttactgatta ttctacttgg
     3961 tttagcttta ttctttttca aggccttgcc tcaagaaatg gctccattag aagatagagg
     4021 cttttttatg gcacgtgtgt ctttgccaga aggccttggt attgcaaaaa ctcgagccat
     4081 tacagaagag attgtcgatg atatgacctc tcgtatagac accttggatt atgaagaaat
     4141 cacggctggt gcaaatggca atagtgccac gatacgagtg gttttaaatg aaaatattaa
     4201 agaacgacgt tcacaaagtg aaattgcaag gcaagtacaa gcgatggggc aagagtatcc
     4261 tgatgttcgt ttaatggtga ttgaacctca agggctttct tctcaaagag gcggaatgcc
     4321 agtcgatttt gttttacaag cgccaagtat tgacgtctta cgcgaattag tcccagtatt
     4381 tttagaagaa gctcaaaagg atcctacttt ctcagtggtg aactcggatt tgaaatttac
     4441 aaaaccagaa ctccatattg agatactaag agataaggct agagaatcgg gagtctatgt
     4501 ggctgacatt gcttctgctg tagaacttgc attaggcgat cagacctatg gcgattttta
     4561 tatgaatggt cgccagtatg atattattgg agcggtcggt ttacaataca gaaattctcc
     4621 agatttgata cgcactcttt ctgtaaaaaa taataatgga gaaattcttt cgctggataa
     4681 ttttgtaaca ctcacggaaa gagctgcggc cccttctctt cctcgatata atcgttttag
     4741 ttcagcgacc atttcagcgg gccttgtgca aggtaaaacg gtgggagaag gagtagaggc
     4801 catgcgtgcc atcgcttcta gaatcttaaa agattatcca acagtttcta ctgatttagc
     4861 tggaacttct aaagaatatg aagaaagttc ttcagggctt ttactcacat ttttacttgc
     4921 gttagcgttt gtgtttttag ttcttgcagg gcaatttgaa agtttcagat ctccgtttat
     4981 tattcttttc acagtgcctt tagctttagt gggagcttta tctgctcttt atgctgttgg
     5041 aattacaatg aatatcttta gtgaaattgc tttaatatta ctgataggat tagtcactaa
     5101 aaatggtatt ttgattgtag aatttgcaaa ccagattgca gagagtacgg gatgctctcg
     5161 tttagaagcc gctcgtttgg ctgcggaacg ccgtttcaga cctattttaa tgacaagttc
     5221 ctctactatt ttagggtctg ttcctttaat catgacagga acgcctagcc gtgtttctat
     5281 gggcgtagcg attgtgggtg gtcttacttt tgcaacgtta atgtcattat ttgtagtgcc
     5341 tgccgcttat tccttctttg ctggcttggt tgaaaagaaa tctttggatg cttctaaaat
     5401 gacctgattt ttattacagc tcatttggaa aattcaaaat tattattctc cgaagaattg
     5461 gcacaaatgg ctttttagaa aaatagtttc gttggaattt atcaagcaaa caagaaaaag
     5521 agcttttact attcgtcggt tggtaaggta gctggttttt cttttttgat atctatgaat
     5581 tgtatgggaa gctcatcttt tttgctcatt ccgtaacgcg ttctcacaag atattctaaa
     5641 tagaaagagt ctgtctttaa tcgtttgttt tcgtcttgtt gtttttggag ttccactttt
     5701 aatgagtcaa tttctatttc aagagattga atgtggttat aaaggcggtg ctgccccaaa
     5761 agaccatttt ttccgaatat aagctgagaa atcattatca gtagaaaaga gataatgata
     5821 ataaaaatga tgccattctt ttttttcagc aagaagaaaa ctccatcaga gaaaaatgat
     5881 atctaaaacc atctttggaa taaaccaagc cagctatttc caattctgtc aatatagata
     5941 aaagtacatg gcgcttgaag ggaaaaagct cttgaacctc ttcgattgtg taggtgtaac
     6001 cacccacttt ttcaagtatt ttagtggcgt cttcagaaag tgaaatatct gcttttaaca
     6061 aggtttcttt agaaggatcg tttttttgag ttaaaccgca gtatagccca agatcttcgg
     6121 gcgaaaataa agcttttgca agtcctcttc ttaaaagagc gtttgttccc gatgccgttt
     6181 cacgattaaa gtctccagga atcgctagaa gagttctttt ttcttttaag caaagatcgg
     6241 ctgtaattaa ggctcctcct tttgttttac tttctataac tacggttgtt ttagaaagtc
     6301 caacaataat accattacgc aaaggaaatt gccatttgaa cgctggagaa tcattttcaa
     6361 aagtactcac taaagcgcct ccctgttcaa taattttttg agcaagtatt ttacgagatc
     6421 cacctatttc tgaatttaat ccctgaccta aaacagcgat tgtaggaatc ccgtatttta
     6481 aggctgcttc atggcaaagg ctatcaatcc cttgagcaag gccagatatg acaaccgcat
     6541 ttgttcccct taagcttttg acaagtaaat cgcataaaag cggagcactt acactaggtc
     6601 ttcgtgtgcc tactaacgcg actcctacag tatttttatc aggaagcgtt cctttaataa
     6661 aaagactttt aggacagtga attgactttt gaagtgattc tggaaagtca ctcgggaaaa
     6721 tttgtctact attactatct ttgtttttca tggcatattc ctttaatgat ttaattgata
     6781 taatgaaacg ccttcgtgct cccgatggct gtccttggga tagagaacaa gacattaatt
     6841 ctttgctccc atatcttata gaagaatctt gtgaatatat agatgcggct caagcaaatg
     6901 acaaagtaca catgtgtgaa gaattgggag atgttctttt gcaggtggta tttcatgctc
     6961 aagtagcctc tgaacagggt gacttttcta ttgacgaagt cgtagaaagt atctgtgaaa
     7021 aaatgattag acgacatccc catgtctttg gggacgccac agcagaagat gccaacgcgg
     7081 ttttacgcga gtgggaacgc attaaagcct ccgaaaagaa taatattgga atggcagaaa
     7141 aatctgccat ggataaggtg agtaaaagtt taccaccgtt ggccagagct caagaattac
     7201 aacgtcgtgc cgcaaaagtg ggctttgatt ggagtcgtga agcaccagta tttgaaaaag
     7261 ttcttgaaga aattacagaa ttccgcaaag aaattcaagc agaaaacaag aataaagaaa
     7321 aaattgaaga agaattcggt gatattttat tttcgcttgt taattgggca aggcatttag
     7381 ggtttaatgc ttctacggct ttaatgagat ccaatgcaaa atttgataga agatttcgcg
     7441 ctgtagaaaa attagcaggt gatcaagata tgaagtccat ggggcttgaa aagctagatg
     7501 ctctttggga cgaagtaaaa aaagaagagc attaaggtaa ccccaaaaag gttatttaaa
     7561 aatggactat gacattaatt taaaataaga ttattgactg ttagcccaat gaaaaaagcg
     7621 atgagatagc cgtaccataa aatagttaga gggtagttaa gcactttttt cccagtacta
     7681 tcaatggatt tattcttttc agcaatgcga actagatttt ttcgcaaata taaaatgctt
     7741 aaaccaagac cgacaataga aacaataatg agtaaaatga tttttatcat aaggcacaag
     7801 atagaaaatt taaaaatgat aggataaaag aataaaaggt ttcttaatag acgatcacta
     7861 attgatttgg ttttttgctt ttttggagta gatgagtaga atattttttt cgtctttatt
     7921 taaatagata tctaaaaaat acaggtagag acctgttttt tgctatttga ggctcttaag
     7981 ggactaaata tgcatctaaa accccttgaa aataaaagta gtgggttgca gttaacgcta
     8041 aaaatttctc tatttgggat cgattaggtc gttcatgtga gcgaaccttt cttttatgag
     8101 gtctttttgg ataaccggga aaaattagat tccctcattg cagatgcttg ccagtctgtt
     8161 ggagttgaac ttgtggagtt agatcaattt caagcgggca aaagaaaagt tttaaggctt
     8221 tatgtcgata aacccgaagg cgtgaccatt ggcgactgca ctgaggtcag ccgtcagtta
     8281 tcggcggcat tagatttaga tgaagaaatt attgctgggg catacacatt ggaagtgtcg
     8341 tctccaggtt tagaccgccc tcttaagtcc acggctgatt ttcagcgtaa tatggggcgt
     8401 cttttacgta tcactcgtag cacagggaag cctgtcactg gaatcctcca gagtgtagat
     8461 gaacaaaata taatgctgga tctaaaaggg catgcagggg agttccaagt tcctcggtcc
     8521 gaaattcttt cagccaaggt agatattcaa ttctgacaaa aggttactat gaaaaacgaa
     8581 cctaaaataa atttagtaga agctttaaaa tcactagtgc aagataaaaa cgttgaagat
     8641 tcgatagtga ccggtgcact gaaggatgcg ttaatttctg ccgctcgtaa gtatttacaa
     8701 atcaataaaa aaatcgatgt agatattgat acagaaaccg atgaaattcg tgttttctta
     8761 agagttgaag tggtggatga ttatcctgac tacgatactg aaatgagtgc tgaagaagtt
     8821 gaagaattgg atgaaggcta tatgcttgtc caagaagctc aagagttcaa tgaagacgct
     8881 cagccaggtg accttttgga aatggaggtt cctgtttctg catttggtcg tcaagccatt
     8941 caaactgcaa agcaattctt aattcagcgt attcgtactg cagaacgtga caaaatttac
     9001 gagacatacc gcagtcgtat tggaaccatg attaatggtg aagtcgttcg tattgaaaat
     9061 aaaaacttga ttgtttctat tggtcgtcaa acagaagctg ttttacctgc taaggaacaa
     9121 attgccaaag aacgtttcca tcaaggaagc tcagtgaaag cggtgattgc tgatgtaact
     9181 gattctacaa agggtggcgc tcaggttatt ttatctagag cgaatggtga cttcttaaaa
     9241 gaacttttcc gtcaagaagt tccagaaatt tatgaaggga ctgttgaagt aaaaggtgtt
     9301 tctcgtgatc ctggatttag ggcaaaaatt gccgtatatt ctcgtgatga acgtattgac
     9361 ccagtaggtt cttgtgtagg catgaagggt gctcgtgtac aagcgattgt tcgtgaatta
     9421 gcaaacgaaa gaatcgatat cgtacattgg aatcctgatt ttgaaactta tgttcgtcgt
     9481 tctttagctc cagcaaatat cgttaagttt gttgaagtct caggcactcg tcgtatagtt
     9541 cttgtagtag ccgatgaaga tcttgctcaa gccattggtc gcaatggtca gaatgtacgt
     9601 ttagcatccc aattagttgg gcgtgattta gacgtgttcg gcgaaaaaga atggtctgaa
     9661 aaagatgaag aaacgaaaga aaaaatatta actcctcgtg ctaatgaata tcgtcaaatg
     9721 aatcaaatga acgataaaaa aatgcagtta gatgagttat ttacaaaaga aaatacagaa
     9781 gaacagccaa tcgaagaaga tggcgtcagt atggaagaaa gtgaggctta atgataagcc
     9841 tattggagcc aacaaataga tgagtaaaat agaagaaatt agacctgcag attgggctaa
     9901 atcaaagggc gtaaccagtg agattgttgt taagctgctg aaagaagcag gtgttgacgt
     9961 tcgttcacac atgacaaaaa ttcctgtttc agaatatgct ccaattgagg cacaggttga
    10021 atccgaaaga gcaaaactgg attcgagaaa acaaaaaaag agtagtagca cttctgatgc
    10081 ggataattct tctacttcat ctgctccaaa gagaaaagtg ggttcggttt ctacaattaa
    10141 gggcggagca gttaaagttt ccctaaaaag agcaaaacca gctgcaccga agccagcctc
    10201 tgagacaaag gctctagcag cacctcagaa acctgctatt gcacaacctg aagtggtaaa
    10261 atcggtagaa tcaaaaaaag agaatgtagc ggtctctaca gcggctgagg tttcaaagcc
    10321 tgtagaacaa aaaccagtgc aacaaaagcc agtagaacaa aaatctgcag agcacaagtc
    10381 agtagaacaa aagtccgctg aacataaacc tgcagtggct cctagaacaa caccacaagc
    10441 ttctttacaa ccgaaaaaag aagtggctcc aaaagaaata cctgctactt ctaaagaaga
    10501 agattctaag ccagacttaa aagtcgctgc aatgaaagct caagttttta aacctgatgc
    10561 agctatttta gctcgtatag aaaaatctcg tcagcaacaa gccaatacac aacatcgtcg
    10621 tccaaatgga cctcgttctg gtggtaataa tagtcaaggt tatacgggtt catttgggaa
    10681 atcaccagct ggtggtggcg taaactcaaa taaccgtcga cctcaaggtc aaggcggaaa
    10741 tgcgggtgga ggttatcgtc caggtggtaa tgcaggcggt ggaaatcgtc cttatacacc
    10801 aggtggtcag aatcgttctg gaggtttttc cagcggttct atgaaagaag cctttggcgc
    10861 ttctcctagt ggagcaaatc ttgttgcctc aacaactaca gcggcaaaag atggttctaa
    10921 atcaggtaat cgtcgtaatg ataaaaaacg tggcacgtct ttaggtaata agcaagaacg
    10981 tcaaaaggaa caacaagacg ttgttcgtca aaatgtttca agagtgatgg cttctctttc
    11041 aaagcatccg atcaagaagg tatatcgtaa agaactttct gaatcggacg gctctgtgga
    11101 acgtcaaatt cttaaaacct cagactttat cactgtgggt gaattagctg ggttaatgga
    11161 acaaatgcct gcacgtgtga ttgctaagtg tatggaaatg ggcatgatgg taaccattaa
    11221 tgctcgctta gattttgaaa gtattcaagt tcttgccgat gaatttggct ttgaagctca
    11281 attgatggaa gaatatgaag aagaagtttt aggtgttgaa gaagaacaaa ccgaaaactt
    11341 attacctcgt cacccagtgg ttacagttat gggtcacgtt gaccatggta aaacctcttt
    11401 actagactgg attcgtaaag cgaatgtggt ctctggtgaa tctggtggta ttactcaaca
    11461 tattggtgct tatgaagtaa gtacaaaagc gggtaaggtt actttcttag atactccagg
    11521 tcatgaagct ttcagtgcta tgcgtgctcg tggttctcaa gttacagacg tgattgttct
    11581 tgtggtggcc gccgactcta tggtgatgcc tcaaacagtg gaatctattg aacttgctca
    11641 tcgcgaaaaa gtgcctttag tggttgctat tacaaaaatt gatttaccta cagccaaccc
    11701 agataaaatt cgtacgcaac tcgctgaacg cggcgtagaa gtcgaacaat ggggtggtac
    11761 tgtcagctgt atcgaagttt cagcacgttc tggtaaagga atggatcttc ttttagaaac
    11821 acttgcttta gaaacagaag tgcttgaact aaaagccaat ccagatactc gtgctcgtgg
    11881 agccgttgtg gagtctaagc tagacgctgg taagggttct atggctacta ttttgattca
    11941 aaatggtact cttcatattg gagatccttt tgtttgcggt ctttattctg gtcgcgtgag
    12001 agcgatgttt gatgaacgtg gtggtattcg aaaagaagcg gggccatcaa ccccttgtca
    12061 agttcttggc tttgacggca ctcctcaggc aggtgatgat cttatcgttg ttgaagatga
    12121 aaagacagca cgtgaaattg catcaaaacg ccgtatggct gcacgtgaaa gagatttacg
    12181 tgctcgtaag acaatctctc ttgaaaatgt ttacgaaggc gccaaagacg gtaaaatttc
    12241 tgagttgaat ttgatcgtaa aagcggatgt gggtggatct gcagaagctc ttgctgcaag
    12301 ccttgaaaaa ctcagcaata gtgaagtaaa gatccatatt attcgtaaag gtgttggtgc
    12361 cattacggat tccgatattc ttcttgctac aacatcacaa gcgattgtca ttgcgttcca
    12421 tttaatgcct tcactttcta ttcgtgaaat ggctgaaaaa gaaggcattg aaattaaaac
    12481 atatcgcgtg atttacgact gtattgaaga tattaagaat gctgtggaag gcttgttaaa
    12541 gcctactacc cgcgaagaaa ttgtgggtga agcagatatt cgtgaagtct ttaagattcc
    12601 aaaggtcggc ttgattgctg gctgtatggt cacgagcggt gaagtcaatc gcgatagtcg
    12661 agttcatgtt tatcgtaacg gtgttgaact tggtcttact gaagttcaat ccttaaaacg
    12721 ccataaagat gatgtcaaat cggtggctcg tggatttgat tgcggtattg gtcttaaggg
    12781 atacgataat atcgaagtgg gcgatacctt gatgttcttt aaggatgtta aagtcgctag
    12841 aactcttgct gatgtggctc gtgatgaaaa ggctgctgct gagaaatcag ctgctgacaa
    12901 ggctgcttca gaaaagaaac aaaacgataa aggtgaaact aagtgagtcg tagaacagac
    12961 agattaggtg aactttttcg aatagagata agcaaactct tgcaaaaggg gttgaaagac
    13021 cccagagttg gctttgctac tattaatcga gtagatatca ctgaagattt aagctttgca
    13081 aaagtattag tctctgtgat gggttccgat aaagaaaaac gcgataccat cattgggtta
    13141 cgtagttcag ct
//
LOCUS       DAOOBB010000040        12843 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_6292_length_12843_cov_4.506021, whole genome shotgun sequence.
ACCESSION   DAOOBB010000040 DAOOBB010000000
VERSION     DAOOBB010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 12843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12843
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6292_length_12843_cov_4.506021"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..2657)
                     /locus_tag="PLT31_03185"
     CDS             complement(210..2657)
                     /locus_tag="PLT31_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HPW94173.1"
                     /translation="MHCNSFIKVLVSGCILFVLGCSEDPQKPEEIDSSSSNLSNGASS
                     GAPLSSGIVNTSSSSNVGSSSSTGVGTSSSSIVPNDSTVTWVGQSSLLITEVNPINYI
                     WLDETGSDPGWVEIYNAGSETANLNGYSLVENLVEPRKWVFGDEVIGPKQFRTVFCDN
                     LDLRVAKEGTDTDTTHFRTHTNWKLEKDGGSIYLIDYNWNIRDSVKYPVLEPGVSWGR
                     VDGGSWKYFAKPTPEKKNTESDPYSGVTNPVTFGDRQAGFYSEPITLNPPTSTDGASV
                     RCTFDGSVPDKNTPVFSSSQFLEKSTVVRCGAFKDGLLTKTIVTNTFFISETVSMPVL
                     SIAVDPYDMFDSANGYYSQGIASCAEPCYDANYFKDIELPVHVEFFESANVGTPRAWN
                     INAGLSIMGGWSRYNDKKSVSISMREQYQDGRLEYPLFETRPDAKKFKAFNLRNNGNR
                     FHWDFVEDAMGGALLEGSGVDYQRSRQVIVFYNGEYFGIHDLRERLNEHFVETNYGID
                     ASTVESIKHTNRLVTANGGTIDGYVAMLDFVDASDFSEAGSTAYQTIQAMLDVGSYAD
                     YMAAQIFYRNGDWPNNNVRAWRSAPEHPWKFMVFDLDHGFNFEWTVPGFGTWINMFDW
                     IKEGGGGESSCLNKGCFAQIFNKLILNPDFKRLFINRSAVMLKNYLTSARIDSITDAM
                     VSKIPEAEMDRDIERWPRKNSVSYTGSALKSFGAERETSAWEEYRSEFDLSSDVTIAI
                     QSTGSGIVLMEGMTLPPRNATKTNYTGTFFGGIDMQLTAIPTQAGATFVKWEDGSTAN
                     PRLVTPTEGATYIATFK"
     gene            2848..2920
                     /locus_tag="PLT31_03190"
     tRNA            2848..2920
                     /locus_tag="PLT31_03190"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2881..2883,aa:Phe,seq:gaa)
     gene            complement(3000..4103)
                     /locus_tag="PLT31_03195"
     CDS             complement(3000..4103)
                     /locus_tag="PLT31_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HPW94174.1"
                     /translation="MNESKNESNTEEILNLLFSYMPKIAEERKMDGLLILMADLGRSL
                     VGADRCSLWLIDENKGEMWTKVAHGVDELRIPMNLGFVGHTVKTGESLCIKDAYKDSR
                     FDSRSDQKTTYHTQSVLTVPLKNSEGAIIGVFQAINKKNPPYYFSPKDVEYLSLTAVY
                     SGKTLESALLTKELEDTQAEILNILGEVSEHRSKETADHVTRVAASCYKMACYLGLSE
                     YEANQIRLAAPLHDLGKVAIPDAILQKTGKFTPEEYEVMKTHTVMGHHLLKSSSRKLL
                     QLAAEMAIGHHEHWDGSGYPKGLSGENIPLSSRICAVVDVFDALSSARCYKEAWSEEM
                     VKDFFIKQKDHQFQKELIDILLLHWDDFISEYK"
     gene            4187..4267
                     /locus_tag="PLT31_03200"
     tRNA            4187..4267
                     /locus_tag="PLT31_03200"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4220..4222,aa:Ser,seq:tga)
     gene            complement(4272..4979)
                     /locus_tag="PLT31_03205"
     CDS             complement(4272..4979)
                     /locus_tag="PLT31_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HPW94175.1"
                     /translation="MPLVRRISNFIFGSSCLGCGLFTEKLDPWLCPSCREELISLGKE
                     PFFPQEDVVCLYTMNSLTRRLVHALKYHHMPGLASYLVKQSAACRGREVFMWFDLLKK
                     PFLLIPIPLSSSRYRERGYNQTEKIAFALASHLEGVVFHGVVRRSFSESQTKLSVEER
                     KRNVAGVFELKKKIPEGRSTCIIIDDVYTTGATTKACSYALLQENKLDVKICTLLFEK
                     PVSAKLDLVADHDVEWD"
     gene            complement(5032..6288)
                     /gene="rodA"
                     /locus_tag="PLT31_03210"
     CDS             complement(5032..6288)
                     /gene="rodA"
                     /locus_tag="PLT31_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HPW94176.1"
                     /translation="MRTGRRFLDKTMKVDWFFILLVLALMCCGVTLVYSATYNPDEVF
                     YQSFWFRQIVYFIVGSFIAAGLAFIRIDYLKRLALPFYFLSLIGLVIVLFGGGDVVKG
                     AGRWIDLGIIKIQPSEFAKIAYLMAFSYWLSRHPVSFFRLKSFLAPFALFIVPFILVL
                     QQPDLSTALVFTAITLVGFYWAGLTVVDMFLLISPVFSVILSHSQFAYSQIFWGVLIC
                     LVALALFYRRLPKALTSFFFLINIFAGYASSMVWNVLKPHQQKRVETFLDPMNDPLGD
                     GYQVLQSMTAIGSGGLTGKGFGEGSQTNLSFLPEEHTDFIFSVLGEQFGFLGAVFVLT
                     LFFLFLWRGLTICKIYEDPFVNLMTVCACAIFLFHILVNIAMTIGLMPVTGLPLPFLS
                     YGGSFALTCMVLVGFLVNMRYQGGRY"
     gene            complement(6285..8141)
                     /gene="mrdA"
                     /locus_tag="PLT31_03215"
     CDS             complement(6285..8141)
                     /gene="mrdA"
                     /locus_tag="PLT31_03215"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HPW94177.1"
                     /translation="MYNASFGNEELQNRNLKVLVLLALIFFFFTIIISRLFYLQYLHY
                     EENLERSENNRLRRVVLTAERGFIYDRKGRILVRNRPSYQIALQSLSLPRKKSEKDSI
                     FRRLLEIRDLNGVQLFDSASLDTAFQRTVWIKNRPIRILEDATPEEVALIEEHSGDLP
                     GVVTLIESRREYPYGTLAAHFLGYTGEISEEQLKQEEFKDYSLGDRIGQKGLELQYDR
                     EFRGVNGVKIIEVNASGREVGVIEGMGSQEPVPGMHLVTTIDLDLQRVAEEAFPDSVR
                     GAVVAIDPRTGEILAMVSSPRLDPNIFSLKRRERNRGWAEVALDSTRPLTNRAIAGAY
                     PPASVFKLVTAGAGLEHGLIGRNTHFQKACTGGFQFGNRYQKCWSSHGNLNLIHALRL
                     SCDVYFYQAGLLIDMPRINEFGERFGLGSDLLGIDIPGERSGWLPDSISFNKRNKRNG
                     WRWARGLILNLAIGQGQLVTPLQQAVFIGSLARNEGVFRPHFMKEIRSIDGALVRRQE
                     PVKIREGVMKPETHQVLLDAMDSVVNHIGGTGKRGAVKNIRVGGKTGSGEWKKGEKTH
                     AWFASVAPLDDPKIAVAVIMEAAGGGGAMAAPVAQKIMQEFFYGDTLKKEGP"
     gene            complement(8134..8625)
                     /gene="mreD"
                     /locus_tag="PLT31_03220"
     CDS             complement(8134..8625)
                     /gene="mreD"
                     /locus_tag="PLT31_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HPW94178.1"
                     /translation="MIWLWIRFPLLFLVAVVMQTTVSKWIRIFDIGPDFIIIFIVLIA
                     LRRGPVVGALWGFFAGFALDVYAPVEWLGAHTISMVVLGFAVGQLEERFLTLNLGMKI
                     GVLGLGFFICDIVYYLAVGFERGIVFNLLLTQTLPESLYTMVCGGVAFKFLFEGNLKN
                     KHV"
     gene            complement(8622..9446)
                     /gene="mreC"
                     /locus_tag="PLT31_03225"
     CDS             complement(8622..9446)
                     /gene="mreC"
                     /locus_tag="PLT31_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HPW94179.1"
                     /translation="MFKITRFLKAIASIGHGLVVFIFLLFLAVIIRKADVMVKNSIVT
                     TALSTIFYPVQSVVSSIDDFYGKSIENEQLKRENAKLRVELFRAKEGLQELSRLHKLV
                     RFENQWDYPIVTARVVGRNPGRFLTTFIINRGKNHGLKEEMPVFSMRGLVGKISKVEW
                     NHASVQLLVDPNLKLSVMEKRTRVIGFLESVKGRNLSAMIPTNAGVKTGDTLITSGLG
                     GIFPKGIEVGVVDDIHKSDLEVMRYMDILPFQEFSLLEEVFVMQKEPDWIVREMLK"
     gene            complement(9469..10491)
                     /locus_tag="PLT31_03230"
     CDS             complement(9469..10491)
                     /locus_tag="PLT31_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPW94180.1"
                     /translation="MFGLFSCDIGIDLGTANTLVHVAGQGIVINEPTVIAVDSKNNRV
                     SAIGFEAKKMLGRTPGESRAVRPMRDGVIAEFELVEVLLQTFIKRVQKYPLFLVKPRV
                     VIGVPSGITEVEKRAVIDAAKMAGAKEVHLLHEPMAAAIGMGIPVEDPIGNMIVDIGG
                     GTSDIAVIALNGTVCNASVRVGGDEMDESVIRYLKSMYNLCVGESTAELIKTQIGSAF
                     PLEKELTMEVKGHDFIAGVPRTMVISSEEIRDALNEPVTAIIEAVKQALSITPPELSA
                     DILDKGIIMTGGGSQLRGLDARIRQETGLAVNVIDEALTCVCKGAARVLEDLDKYRPV
                     LIASSN"
     gene            complement(10553..10915)
                     /locus_tag="PLT31_03235"
     CDS             complement(10553..10915)
                     /locus_tag="PLT31_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94181.1"
                     /translation="MQKIIFIILSVFFFGCSEAPERIESKLHPYLQEDLKFMVAELLK
                     ANGTKEDLLDTPYYVVKDFRLFEGASAEIFSAYAEVDFFIYKKIKLYQKRKYRYDAHY
                     RKWDRYYKKFFFGSDFSK"
     gene            complement(11025..12317)
                     /locus_tag="PLT31_03240"
     CDS             complement(11025..12317)
                     /locus_tag="PLT31_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94182.1"
                     /translation="MNFRSAVVAGVAFGVIGVGSAFATFARVESMGKNTTYIMDDVSI
                     FDNPANINFYPNYLIGEFGNYTDDVPAGTNLDPKTPTFGGIFAVPLGASSSPDARISI
                     GGLFGRLDTELTKYLPTRFVGDNVRDTIDLPNTVTNFDGFLGGTFSNGNAIGLHIYIA
                     HQDGGYETPSGVYQVDKDAYASVVRVDAGINWQFRNSIDFEASFGLARIQYGPEHKGF
                     FDDGDFSYLGKARAFFTLEAIDGELVPAASGSFIQSPGIRNIHAQAGLGINVALDRGF
                     FWLGADFVWNQLKAHDWEFDYTTKESTYNSQYSDELMWDKRQDVGGQISFGIERNIWY
                     DWFVIRVGGKKSILYTSCDQDPRNVGKSGVCLENGNFFSTNPLADGSSDDHVGFGFGI
                     NIEEKLKIDVTVAEDLLYRNPFQGEGRFFSRISASYSF"
     gene            complement(12438..>12843)
                     /locus_tag="PLT31_03245"
     CDS             complement(12438..>12843)
                     /locus_tag="PLT31_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HPW94183.1"
                     /translation="GIGDGLAGLLGGGGGGIATKARGSIKTPSERDIDMGAGGGSRSA
                     ADIMKVVRQRTPGLRHIYNKSLKKKPGFAGKVTLRFTIAPGGEVISISIASSTTGFSE
                     FDNDVKSAVSRWTFSKVKSGNTTVTIPFTFSE"
BASE COUNT         4410 a         2877 c         2012 g         3544 t
ORIGIN      
        1 cgaagcatcc gacaactagc cttacgccaa gccacatctc taaaaagact caaagcatga
       61 tttaaaaaat cataccgatg agcaatacca tcaaacattt ttcttatcgg actaaccata
      121 accctaaaga taaaaagaca acgcccgaga cgttgccttt ttaaaataaa atactgtttt
      181 tctattcaat aatagatgaa aaagaaatgc tacttaaatg tggcaatata agtagctcct
      241 tctgttggag tgactaaacg aggattcgct gtggagccat cttcccactt cacaaaagtt
      301 gctcctgctt gagtaggaat agccgttaat tgcatgtcta tcccgccaaa gaacgttcca
      361 gtataatttg tttttgttgc atttcttggt ggtaaagtca ttccttccat caaaacaatt
      421 ccgcttccag tggactgaat tgcaatggtc acatcagaag ataaatcaaa ctcagaacga
      481 tattcttccc atgcgctcgt ttctctttcc gctccaaaag actttagagc agaacccgta
      541 taagaaacgc tatttttacg aggccagcgt tcaatatctc ggtccatttc tgcttctgga
      601 atttttgaaa ccatagcatc agtaatagaa tcgattcgag cggaagttaa ataattcttc
      661 agcatcaccg cagaacgatt gataaagagt cgcttaaagt ctggattaag tatcaacttg
      721 ttaaagatct gggcaaaaca gcccttattt aaacaacttg attcacctcc accaccttct
      781 ttaatccagt caaacatgtt aatccatgtt ccaaaaccag gtacagtcca ttcgaaatta
      841 aagccatgat ctaaatcaaa aaccataaat ttccatggat gctctggagc agaacgccat
      901 gcacgaacgt tattatttgg ccagtcccca tttctataaa agatctgagc agccatataa
      961 tcagcataac ttccaacatc taacatagct tgaatagttt gataagcagt gcttcccgct
     1021 tcagagaaat cacttgcatc tacaaaatca agcatcgcta catacccatc gattgttccg
     1081 ccattggctg ttaccaaacg attagtatgt ttaatagatt ccaccgtcga ggcgtctatg
     1141 ccataattag tttctacaaa gtgttcattt aagcgttctc gtaaatcgtg aattccaaag
     1201 tactctccat tatagaatac aatcacttga cgagatcttt gataatcaac accagagcct
     1261 tcaagtaaag cacctcccat tgcatcttcc acaaaatccc aatggaagcg attaccatta
     1321 ttccttaagt taaacgcctt aaattttttt gcatctggac gagtttcaaa aagtgggtat
     1381 tccaaccgac catcttgata ctgctcacgc atagaaatag aaacagactt tttatcatta
     1441 taacgactcc agccgcccat aatggaaagg cctgcattaa tattccatgc tctaggagta
     1501 ccaacatttg cagattcaaa aaattcaaca tgaacaggaa gttctatatc tttaaaataa
     1561 tttgcatcat aacaaggttc tgcacaacta gcaatgcctt gagaataata cccattagca
     1621 gaatcaaaca tatcataagg atcaactgca atcgaaagaa caggcatgct cacggtttcg
     1681 ctgataaaga acgtatttgt tacaattgtc tttgtgagta agccatcttt aaaggcgcca
     1741 caacgaacaa cagtcgactt ttctaaaaat tgagaacttg aaaaaacagg cgtgttctta
     1801 tctggaacag aaccgtcaaa agtacaacga acagaagcgc catctgttga tgtaggagga
     1861 tttaatgtga ttggctcact ataaaatcct gcttgacgat ctccaaaagt aacgggattg
     1921 gtgacaccag aatatggatc tgactctgta ttcttttttt ctggagtggg ttttgcaaaa
     1981 tacttccaac taccaccatc tacacggccc cagctcacac ctggttctaa aacaggatat
     2041 tttacagaat ctcgaatatt ccaattgtag tctattaggt aaatagaacc gccatctttt
     2101 tctaacttcc aattcgtatg cgtccggaaa tgcgtagtgt ctgtatctgt cccctcttta
     2161 gccacacgta aatcaagatt atcacagaaa accgttctaa attgttttgg accaatgacc
     2221 tcatctccaa aaacccattt acgaggttcc actaagtttt cgacaagtga atacccattc
     2281 aaattagccg tttcagaccc agcattataa atttcaaccc aaccaggatc tgaacctgtt
     2341 tcatccaacc agatgtaatt aattggattc acttctgtaa tcaataaaga agactggccc
     2401 acccaagtta cagttgaatc attagggaca atggaagaag aggaagttcc aacaccagtc
     2461 gacgaagacg aaccaacatt agaagagctt gatgtattta ctatgcctga agaaaggggc
     2521 gctccagaag aggccccatt agataaatta gaactactag aatcaatctc ttcaggtttc
     2581 tgtggatctt ctgagcagcc taaaacgaat aaaatacaac cagatactaa aactttaata
     2641 aaagaattac aatgcaaaat aaccacctat cgaaagcttt ctacacccat atgccaaaac
     2701 ctttcatttc aaatctatat gaaaaaataa tttaaaatta atacttaaca gtaatattct
     2761 gtttacacct tttttttctg tattttctat cactttccct tgacacattg aaaattctgt
     2821 gttaaatttg gcatctatca acagaatggc tctgtagctc agttggtaga gcagcggact
     2881 gaaaatccgc gtgtcagcgg ttcaattccg cttggagcca ctcaacaaaa aaacttcact
     2941 tttagtgaag tttttttgtt ttatgtcaaa tttttgaaaa aacagactta ttagcgggtt
     3001 tatttatatt cgctaataaa atcatcccaa tgtaacaata aaatatcaat caactctttt
     3061 tgaaactgat gatctttttg ttttataaaa aaatctttca ccatttcttc tgaccaagct
     3121 tctttataac aacgagcaga actcaatgca tcaaaaacat caactaccgc acaaattcgg
     3181 ctagaaaggg ggatattctc accagaaagt ccttttggat atccagaacc gtcccaatgc
     3241 tcatgatgac ctatagccat ttctgccgct aattgcagca attttcttga agaactcttt
     3301 aaaagatggt ggcccattac agtatgagtt ttcatcacct catattcttc aggagtaaat
     3361 ttccctgttt tttgtaaaat cgcatcagga attgcaactt ttcctaaatc atgcaacggg
     3421 gcagccaaac gaatttgatt cgcttcatat tcacttagcc ctaaataaca agccatttta
     3481 taacaagagg cggcgactcg cgtgacatga tcagccgttt ccttactgcg atgttcagat
     3541 acttctccta aaatattgag tatttcagcc tgtgtatctt ctaattcttt cgttagcaaa
     3601 gcagattcta gcgtttttcc agaataaaca gccgttaaac ttagatactc cacatctttt
     3661 ggagaaaagt aataaggagg atttttttta tttatggctt gaaaaacacc tataatagcc
     3721 ccctccgaat ttttcagagg cactgtaagt acggattgag tgtggtaagt tgttttttga
     3781 tcgcttctgg aatcgaacct agaatctttg tacgcatcct taatacaaag gctttctcca
     3841 gttttcacag tatgcccaac aaagcctaaa ttcatgggaa ttctaagttc atctacccca
     3901 tgagccacct ttgtccacat ttcgccctta ttttcatcaa taagccacaa tgagcaacga
     3961 tccgctccaa ccaaagaacg acctaaatcg gccattaaaa tcaaaagacc gtccatttta
     4021 cgttcttctg caatttttgg catataggag aacaataaat tgaggatttc ctctgtatta
     4081 gactcattct ttgattcatt cataactaaa atttaaatat aaagattaaa aaaggcatcc
     4141 tgctattgac gaaatgacac ttctttttta tcgttgctgc tattccggag gaataggcta
     4201 attggtaagt cagcggtctt gaaaaccgcc gccgcaaggc ttgggggttc gagtccctct
     4261 tcctccgcta cttaatccca ctctacatcg tggtctgcca ccaaatcaag ctttgcagac
     4321 actggttttt caaaaagtag cgtacatatt ttcacatcca atttattttc ttgtaatagg
     4381 gcataagagc aagcttttgt cgttgctcct gtcgtatata catcatcaat aataatgcaa
     4441 gtcgaccgtc cttctggaat ttttttcttc aactcaaaaa cacctgctac atttcgtttt
     4501 cgttcttcaa cagataattt tgtttgtgat tcactaaaag aacgtcgaac aactccatga
     4561 aaaacaaccc cttcaaggtg actagcgaga gcaaaggcta ttttttccgt ttggttatac
     4621 ccgcgctctc gatatcgaga agaactaaga ggaataggaa tcaataaaaa tggtttcttt
     4681 aacaagtcaa accacataaa cacttctcgt cctcgacaag ccgctgactg ttttacgaga
     4741 tacgaagcaa gcccaggcat atgatgatac tttaacgcat gcactaaacg acgagttaaa
     4801 gaattcatgg tatataaaca aactacatct tcttgaggaa agaatggctc tttaccaaga
     4861 gaaatcagtt cttctcgaca actaggacaa agccaaggat ccagcttctc tgtaaatagg
     4921 ccgcacccta agcatgagga accgaaaata aagtttgaaa ttcgacgaac caacggcata
     4981 aaaaactcct gaaaacgttt tcttatttaa acgttttttc aagagatttt tttaataacg
     5041 accgccctga taacgcatgt ttactaaaaa accaactaag accatgcatg ttaaagcaaa
     5101 agagccccca taagaaagaa agggcaaagg taaaccagtg acaggcatca aaccaatagt
     5161 catcgcgata tttactagaa tatgaaataa aaagatggca caagcacaca ccgtcattaa
     5221 attgacaaaa ggatcttcgt atatcttaca gatagtgagt ccacgccaca gaaataaaaa
     5281 aaataaagtc aaaacaaata cagctccaag aaaaccgaat tgttctccta atacgctaaa
     5341 aataaaatcc gtatgctctt ctggtaagaa cgataagttc gtttgagatc cctcaccaaa
     5401 gccctttcca gttaaaccgc ctgaaccaat agcggtcata gattgtaata cttgatagcc
     5461 atcgcctaaa gggtcattca ttggatctaa aaaagtttct acacgttttt gctgatgagg
     5521 ttttaacaca ttccacacca tggaactcgc atatccagca aaaatattga ttaaaaagaa
     5581 aaaggaagtt aaagctttgg gtaatcttct ataaaacaaa gcaagagcga caagacagat
     5641 taatacaccc caaaaaatct gagaataggc aaactgagaa tgagatagaa tcactgaaaa
     5701 aacaggacta atcaacaaaa acatatccac aacagttaag ccagcccaat aaaagccaac
     5761 caaagtgatt gctgtaaaaa caagagctgt acttaaatca ggctgttgta gtaccaaaat
     5821 gaaaggaaca ataaacaaag caaatggagc aagaaaagat ttcaagcgaa aaaagctcac
     5881 tggatgcctt gataaccaat aagagaaagc cattaaataa gcgatcttag caaattcaga
     5941 aggttgaatt ttaataatac ctagatcaat ccaacgacca gcacctttaa ctacatctcc
     6001 accaccaaat agcacaataa ctaacccaat gagagataaa aaataaaaag ggagcgctaa
     6061 ccgtttcaaa taatcaatcc gaataaatgc aaggccagca gcgataaatg agcccacgat
     6121 aaaataaaca atctgacgga accaaaaaga ttgataaaat acttcgtctg gattatacgt
     6181 tgcggaataa actagtgtca caccacaaca catcaaggct aaaacaagaa gaataaaaaa
     6241 ccaatccact ttcatggttt tatctaaaaa acgccgtccc gttctcatgg accctccttt
     6301 tttagggtat ccccataaaa gaattcttgc attatttttt gtgcgacagg agcagccatc
     6361 gcaccaccac caccagcagc ctccataatc acagcaaccg caattttagg gtcatctaaa
     6421 ggagccactg aggcaaacca tgcatgcgtc ttttcgccct ttttccactc tccagagcct
     6481 gtttttccgc ccacacgaat atttttaacc gcacctcgct ttcctgtacc tccaatatga
     6541 ttcaccacgg agtccatcgc atctaaaagc acttggtgcg tctcaggctt catcacccct
     6601 tctctgattt ttacaggctc ctggcgtcta actaaagctc catctataga acggatttcc
     6661 ttcataaaat gaggcctaaa aacgccctca tttcttgcaa gagagccaat aaatacagcc
     6721 tgctgtaaag gagtgactaa ttgcccctgg ccaatcgcta aattcaaaat caaaccacgt
     6781 gcccaccgcc agccatttct tttattcctt ttattaaaac taatcgaatc aggcaaccat
     6841 ccagaacgtt ctccaggaat atcaatgcct aataaatcag aacctaagcc aaaacgctct
     6901 ccaaattcat taatccgagg catatctatt aataaaccag cttgataaaa ataaacatca
     6961 cacgaaagcc ttagtgcatg aattaaattc aaattaccat gagatgacca gcacttttga
     7021 taacgatttc caaattgaaa accacccgta caagcctttt gaaaatgagt attccgtcct
     7081 atcaatccat gttcaagccc agcaccagcc gtcacgagct taaaaacaga agctggcgga
     7141 tacgctccag caatagctct attagtcaaa gggcgtgtcg aatctaaagc aacttcagcc
     7201 caacctctat ttctttctcg tctttttaga gaaaaaatat ttggatccaa acgaggagaa
     7261 cttaccatcg caagaatttc tcctgtacgt ggatctatag caacaacggc tccacgaaca
     7321 ctatcgggaa aagcctcctc tgcgaccctt tgcaaatcaa gatcaatcgt tgtcaccaaa
     7381 tgcattccag ggacaggttc ttgactaccc atgccttcga taacaccaac ttcacggcca
     7441 gaagcgttta cttcaataat tttaactcca ttaacacctc taaattctcg atcatattgc
     7501 aactcaagac ctttttggcc aatccgatcg cctaaggaat aatccttaaa ctcttcttgt
     7561 ttcaattgtt cttcggaaat ttctcctgta taacccaaga aatgagcagc taaagtacca
     7621 tatgggtatt ctcgtcttga ttcaattaaa gtaaccacgc caggtaaatc gcctgaatgt
     7681 tcttcgatca aggccacttc ttcaggagtc gcatcttcta aaatccgaat aggacgattt
     7741 tttatccaaa ccgttctttg aaacgcagta tctagagaag cagaatcaaa taactgcacc
     7801 ccatttaaat ctcgaatttc taataagcga cggaaaatag aatctttttc actttttttt
     7861 cttggcaaag aaagtgattg caacgcaatc tgataagagg gtctattacg tactaaaata
     7921 cgtccttttc gatcataaat aaagcctcgt tctgcagtaa gaaccactcg tcttaagcga
     7981 ttattttctg atcgttctaa attttcttcg tagtgcaaat attgtaagta aaaaagtcta
     8041 gaaatgataa ttgtgaaaaa gaagaaaatt aaagctaaaa gtacaaggac ttttaaattt
     8101 ctattctgta gttcttcatt tccaaaactc gcattataca tgtttatttt tcaaattccc
     8161 ttcaaaaaga aatttaaagg cgacgccacc acaaaccata gtgtataagc tctctggaag
     8221 tgtttgagtc aagagtaaat taaaaacaat acctctttca aatcctacag caagataata
     8281 aacaatatcg caaatgaaaa aacctaaacc caagacacct attttcatac ccaaatttaa
     8341 ggttaaaaaa cgttcttcta attgaccaac agcaaaacca agtacaacca tagaaatcgt
     8401 atgtgcaccc aaccattcta caggagcgta aacatctaaa gcaaaaccag caaagaaacc
     8461 ccacaaggct ccaacaacag ggcctcgacg caaagcaatc agaacaataa aaatgattat
     8521 aaaatcaggt ccaatatcaa aaatgcggat ccatttagaa actgtcgtct gcatcactac
     8581 agcaactaaa aataaaagag ggaagcgaat ccatagccaa atcattttaa catctcccga
     8641 acaatccaat cgggttcttt ttgcattaca aaaacttctt ctaatagcga aaattcttgg
     8701 aacggcaaaa tatccatata acgcatcacc tctaaatccg acttatgaat atcatccaca
     8761 actcccacct ctattccttt tgggaaaata ccccctaaac cagaggtaat cagagtatct
     8821 cctgttttta cacctgcatt ggtagggatc attgcggaaa gattacgtcc cttcaccgat
     8881 tccaaaaagc ctatgactct tgttcttttt tccattacag aaagttttag attaggatca
     8941 acaagaagtt gaacagaggc atgattccat tccacctttg atattttccc aacaagtcca
     9001 cgcattgaaa aaacaggcat ttcttctttt agcccgtgat tttttccacg attaataata
     9061 aacgttgtta aaaaacggcc aggatttcgt ccaaccacgc gagccgttac aataggataa
     9121 tcccattgat tttcgaaacg aactagttta tgaagacgag aaagctcttg tagcccttct
     9181 tttgcacgaa acaattcgac acgaagtttc gcattttctc ttttcaattg ttcattctca
     9241 atagacttcc cataaaagtc atctatagaa gaaaccactg actgaacagg ataaaatatc
     9301 gtactaagag cagttgttac aatactgttt tttaccatca catctgcttt acgaataata
     9361 acagctagga acagcaaaaa aataaaaaca actaatccat gacctatgga tgcaattgcc
     9421 tttaaaaaac gggtaatctt aaacatttac aacccgtatt ccagttaatt aattcgaaga
     9481 agcgattaaa acagggcgat acttatcaag gtcttctaga actcgagcag cccctttaca
     9541 aacgcaagtt aatgcttcat caatcacatt cacagctaag ccagtctctt gacgaatacg
     9601 agcatctaaa ccacgtagtt gagagccacc accagtcatg ataatacctt tatctaaaat
     9661 atctgcggat aattcaggag gcgtaatact taaggcttgt ttaacggctt caataattgc
     9721 tgtcacaggt tcattcaaag catcacgaat ttcctcacta gagataacca tcgtacgagg
     9781 aactccagca ataaaatcat gacctttcac ttccattgtc aattcttttt cgagagggaa
     9841 agcagaacca atttgtgttt taattaactc tgcggtactt tcacccacac acaaattata
     9901 catgctttta agatagcgaa ttacagactc atccatttca tcgccaccaa cgcgaacaga
     9961 agcgttacat acagtaccat taagagcgat cacagcaata tcagaagtac caccaccaat
    10021 atcaacaatc atatttccaa ttggatcttc tacaggaatt cccattccga tagctgcagc
    10081 cattggttca tgaagcaaat gaacttcttt tgctccagcc atttttgcag catcaattac
    10141 tgctcttttt tccacttcag ttataccaga tggaacacca atgacaactc gaggtttaac
    10201 taaaaataga ggatacttct gaactcgttt gataaaggtt tgcaaaagaa cttctactag
    10261 ttcaaattca gcaataacac catctctcat tggtctaaca gctctactct ctcctggagt
    10321 acggcctaac attttttttg cttcaaaacc aatcgcagaa acacgattgt ttttactatc
    10381 tacagctata accgtaggct cgttaattac aatgccttgg ccagcaacat gcactagcgt
    10441 atttgctgtg cctaaatcaa taccaatatc acaagaaaat aaaccaaaca aagtaaagcc
    10501 ctcgaataga atttgaaata aaaatatagc ttaaaaataa accaatctac ccttacttag
    10561 aaaaatcaga cccaaaaaag aactttttat aatatcgatc ccatttccga taatgtgcat
    10621 cataacgata ctttcgtttt tgataaagtt tgatcttctt ataaataaaa aaatcaacct
    10681 ctgcataagc tgagaatatc tcagcactag ccccttcaaa gagacgaaaa tctttaacca
    10741 cataataagg tgtatctaac agatcttctt ttgttccatt cgcttttaaa agctcagcaa
    10801 ccataaattt caagtcttct tgaagatacg gatgtaattt tgattcaata cgttctggag
    10861 cctcagaaca gccaaagaaa aaaacactta agataataaa aatgattttt tgcataaaag
    10921 aattcaagaa gacaattaac cttatactta attaagaata ttaagaatat aaaaaaaagg
    10981 tctctcaaaa ggagaccttt ttataaatag attcgcttta cgagttagaa agaataagat
    11041 gctgaaatgc gagagaagaa acgaccttca ccttgaaaag gattacgata caataaatct
    11101 tcggcaacag taacatcaat ctttaacttt tcttcaatat taatgccaaa gccaaaacca
    11161 acgtggtcat ctgaggaacc gtctgctaat ggatttgtac taaagaagtt tccattttca
    11221 agacatacac cagacttgcc aacattgcga ggatcttgat cacaagaggt ataaagaata
    11281 gatttcttac cgcctacgcg aataacaaac caatcgtacc agatgttcct ttcaatacca
    11341 aagctaattt gcccaccaac gtcttgtctt ttatcccaca taagttcatc agaatattga
    11401 gaattatagg tggattcttt tgttgtataa tcgaattccc aatcatgagc tttcaactga
    11461 ttccatacaa aatcagcacc aagccagaag aagcctctat ctaaagcaac attaatgcct
    11521 aaaccagctt gagcatgaat attacgaatc cctggagact gtataaaact accagaagca
    11581 gcaggaacta attcaccatc aattgcttct agagtaaaga aggcacgggc ttttcctaaa
    11641 taagagaagt ctccgtcgtc aaaaaagcct ttgtgttcag ggccatattg aatgcgagct
    11701 aaaccgaaag aagcctcgaa atcaatagag ttacggaact gccagttaat accagcatca
    11761 acacgaacta cagaagcata agcatcttta tctacttgat aaacaccgct tggtgtttca
    11821 tatccgccgt cttgatgagc tatataaata tgaagaccaa tagcatttcc attagaaaaa
    11881 gtaccgccca agaagccatc aaaatttgtt actgtatttg gaagatctat cgtatcacga
    11941 acattatctc caacaaaacg agtaggtaaa tactttgtta attcagtatc aagtcttcca
    12001 aataacccac ctatagaaat gcgagcatct ggagaactac ttgccccaag aggaacagca
    12061 aaaattccac caaatgtagg cgtttttgga tctagatttg taccagcagg cacatcatca
    12121 gtataattac caaactctcc aattaaataa ttaggataaa aatttatgtt ggctgggtta
    12181 tcaaaaatac taacatcatc cataatgtaa gtcgtatttt tgcccatcga ttcaactctt
    12241 gcaaaagtgg caaaggcaga accgactccg atcacaccaa aagccacacc agcgacaact
    12301 gcagatctga aattcattga aaactcctgt aaaaatctta tttaaactta cttttataaa
    12361 tgaagtttag caacataaaa ataaaaaaaa cgaggctgaa aactatgttt caacctcgtt
    12421 cttctaaaac aactcaatta ttcagagaat gtgaatggaa ttgtaactgt agtgttacca
    12481 gacttcactt tactgaatgt ccagcgacta actgcactct tcacatcatt atcaaactca
    12541 ctgaaaccag tagtcgaaga tgcaatagag atactaatca cttctccacc cggagcaatt
    12601 gtaaagcgta gagttacctt accagcaaaa ccaggctttt tcttcaagct cttgttatag
    12661 atatgacgta gacctggagt acgctgacga accactttca tgatatctgc agcactacga
    12721 gaaccaccgc cagcacccat atctatatca cgttctgatg gagtcttgat agaacctctc
    12781 gccttagtag caataccacc gccaccaccg ccgagaagac ctgctagacc atcaccaata
    12841 cca
//
LOCUS       DAOOBB010000041        12642 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_6400_length_12642_cov_4.171923, whole genome shotgun sequence.
ACCESSION   DAOOBB010000041 DAOOBB010000000
VERSION     DAOOBB010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 12642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12642
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6400_length_12642_cov_4.171923"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="PLT31_03250"
     CDS             complement(<1..582)
                     /locus_tag="PLT31_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94184.1"
                     /translation="MSEEIKKTVSDEICENIQKHGFLPIPVQTLNEDAEASRYFAGTF
                     EEFLEAAKALNAKGIFVETLYLEEDEFYYDSGIDEEDDEYDCCCCDDCDCDCDCDCED
                     DCECDDCECDDEECECECTEESDKEEDEAVWIDPEDLDGLDLSLLRPEVANYQDRIGE
                     SCGVRLTIPGVDHVEVEIFAEWYNTFAEMIDDAY"
     gene            complement(598..1554)
                     /gene="dapA"
                     /locus_tag="PLT31_03255"
     CDS             complement(598..1554)
                     /gene="dapA"
                     /locus_tag="PLT31_03255"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HPW94185.1"
                     /translation="MQITKSSQLTGVFPALFTPLMSDDPKNLYNTIDYKKMALMIDDL
                     IESGVSGIVPVGTTGQSATVTHKQHLDIIKFTLDYVNGRVPVIAGAGSNCTRESIEMI
                     QEILKIASVPVLCVTGYYNNPSQEGIEKHFKTLSSETGAKIILYNVPGRTSNYIHPDT
                     LIKLSEDKNIIGLKQAVEFRIGEKYHEDTVRVIKETANNDFAVLSGEDGAFIDILEMG
                     GKGLISASANISEASSIFVQLYRAFLDGHFEKADDLQDAARDFIEITFCRKNPIPLGT
                     LFNSPLFLPLVSVRQTARGDEAEARILKLIHEKAPSLLKYHK"
     gene            1646..3259
                     /locus_tag="PLT31_03260"
     CDS             1646..3259
                     /locus_tag="PLT31_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HPW94186.1"
                     /translation="MQTITQLWKKIQSPDFNPSTDFALVEEIKKEALTSQKPTKVTFG
                     TSGWRGEIGSEFTIRNLQVVGAAIVRLYREATPELLKSLGVLNFEDLQKRGLVIGHDN
                     RLLGKEFCMAVASQFQKAGVKIYYGGEMATPEFSASVELLGAACSINMTPSHNPSHYN
                     GIKFNPADGGPAGPEITDVITALSNEMMNTHQFEPVGEVSFEKIDPVKIYKEFLVKQE
                     TIKFGRIQKLIDEGRLTLVCDHVHGSTRGRPAALLGNPECLISLRTEDDSLFGGVAPE
                     PSSKNLELVRQALDASKNWFRLGAIFDPDGDRIRFYDGTREIDMNSFGAIAFHYMITW
                     RKEQGVVAKSVATSNFVNIIAEKLGVPVMETPVGFKNFRPWLARNARPKALIAFEESD
                     GISGLNNTLEKDAQFGLLLALEIMAVTGKNLGEYLDALYAEYGRFYPTRSGFEVDKSL
                     VGDPLIAKVNAVAERAQPKMKVLVGSEEKEVKQLLTLDGVKIIFTDDSWMLIRPSGTE
                     PKVRIYTECRKPEEKDALFEAAKALFFKE"
     gene            3269..3814
                     /locus_tag="PLT31_03265"
     CDS             3269..3814
                     /locus_tag="PLT31_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94187.1"
                     /translation="MKKIILFLFCLLTTISAQVEPEFQGFAGNLLRLKKANKGYHDFS
                     MEITVPPWAFSVEGSVKSPGGDPDVLFNGIFDEELIVVMAYIPYPTQGKDGNEYQTGM
                     FDLMIYLQDEKTVIKDLSFKLLSPANDSWAKESFEMAKSSSQMLGPIWNGKFEKKIIV
                     ASATPLTKKKIKALQKKMNKK"
     gene            complement(4001..4741)
                     /locus_tag="PLT31_03270"
     CDS             complement(4001..4741)
                     /locus_tag="PLT31_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPW94188.1"
                     /translation="MSGHSKWATIKRKKAKTDVDRAKAWNKLIKEISIAAKLGGDNLD
                     ANPRLRAAVLKAKGQSLPAKNIESAIQKAVGGNSGADMTEPMYEGRGPGGVAILVSCM
                     TDNKVRTVAEVRNVFNKNGGNMGESGSVAWAFTYKGVILIDAEKYTEDKVMDIVLEAG
                     ASDMSTEENVHEVSTTPDAFEAVTKALEAANIEMMSAELAYVPNDTIKLERADAQKLL
                     KLIDKFEDNDDVQDVYHNAEIDVEDMEE"
     gene            complement(4797..5888)
                     /locus_tag="PLT31_03275"
     CDS             complement(4797..5888)
                     /locus_tag="PLT31_03275"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPW94189.1"
                     /translation="MLIKHNSIWMLNLEALANHENYTIEFHPVEKRLSLKGNSSEWVF
                     VVGFPYFLHDGKTESLSAPVYLEKDKVFAPAEETVKFFQKEFNKNITLDTIKNLVLFT
                     DLPKAKPKPIIPQTSKKETAGTREVKTIVIDPGHGGKDPGALGKKSQEKDIVLSVAKL
                     LKKKLTKEGFNVKLTREKDVFIELSQRANLANKWDGDLFISLHCNAVDGIERQKRTEG
                     YHFYVLRAPESEEDKAIARRENKVASLYGDKEAKEELSPLEWFKLEARLEKYKQNSYM
                     YTEELLNSFNNNGKIKQLGTGVGGAGFMVLVGALMPAVLVELGFITHPKDENYMNSAK
                     GQEDLANRITKSIIEYKKAVNDYRKTLQH"
     gene            complement(5945..6418)
                     /gene="smpB"
                     /locus_tag="PLT31_03280"
     CDS             complement(5945..6418)
                     /gene="smpB"
                     /locus_tag="PLT31_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HPW94190.1"
                     /translation="MNKPKEQKTPTIINRKANHLYSVEERFEVGIMLIGSEIKSIRDG
                     KCNLGDAWVDVSSDKDEIWLVGAHIDEYLFANRFNHIPARRRKLLAHLHEIQKMRKAV
                     ETKGKTIVPLKLYFKNRYAKLEIGICRGKDQQDKRQDIITRDAKLEMARIIKMHR"
     gene            complement(6405..6758)
                     /locus_tag="PLT31_03285"
     CDS             complement(6405..6758)
                     /locus_tag="PLT31_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPW94191.1"
                     /translation="MATNNSNITKKDIVEDIALRTGLTQVDTKVIIESFLSSVVKAMS
                     EGNNIEIRGFGRFKIKERKARKARNPKTGEDVIVKAGIKPVFEASRELKKFLNDTPVI
                     DELFSESIESKNEQA"
     gene            complement(6835..7824)
                     /gene="obgE"
                     /locus_tag="PLT31_03290"
     CDS             complement(6835..7824)
                     /gene="obgE"
                     /locus_tag="PLT31_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HPW94192.1"
                     /translation="MFLDEKVIEVRSGKGGDGLCSFRHEIYTPLGGPDGGDGGRGGSV
                     ILAVNEQYSTLLDMGNIRLYKAKSGSPGGASRCTGASADDLIVNVPRGTLVKDEEGRI
                     LADLTEIGQTWVAAKGGRGGFGNQHFATPSNQAPRKIIPGKPGEKRMLYLELKLMADV
                     GLVGFPNAGKSSLVHKISSARPKIGDYPFTTLEPILGIVQLGSRSFVVADIPGLIEGA
                     SEGRGLGHQFLKHIERTHTLLFVIDGFEEDAYEKFKILKKELLGFHPKLAEKPYLIAL
                     NKSDLGIEKTQKIFLKKKEKVILTSAITGEGCKELKIALDEMVPNKQKRSKGW"
     gene            complement(7824..8789)
                     /gene="argC"
                     /locus_tag="PLT31_03295"
     CDS             complement(7824..8789)
                     /gene="argC"
                     /locus_tag="PLT31_03295"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HPW94193.1"
                     /translation="MKIKVFVDGQEGTTGLQIHERLNNRDDIEILEIDSALRKDPEAR
                     AKLINASDITFLCLPDEAAKESALLCTNSKTKIIDASTAHRTNPLWAYGLPELSQKFR
                     DQIRQSHRIANPGCHATAFNLGLYPLTSKGLLPLETPVSAYGITGYSGGGKKLISEYE
                     TEINNAKVAGKESFIFAPSPYALALTHKHLPEMQKYTELKNPPLFSPILGPFYKGMAV
                     SINLFASQFTHKITPESLTEVFQSHYDGCHFIEIMPYEKIPELHHGRLDCTVCNDTNK
                     ARIQIFGNENLMQVTTIIDNLGKGASGAAVQNMNIALGLDEGISL"
     gene            complement(8805..9296)
                     /locus_tag="PLT31_03300"
     CDS             complement(8805..9296)
                     /locus_tag="PLT31_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HPW94194.1"
                     /translation="MSQDLCPCGSLKKYSECCEPIIKKAEKASSPEVLMRSRYTAYAK
                     HEISWLKDSLDPTQREEFDEKSVEAWSRESEWVGIEIRNTKTEEEKNIGWVEFIARFK
                     QGNITRNHHELGEFHKINDEWFFYDGRAVKPETVKKEGPDVGRNDPCPCGSGKKYKKC
                     CGQ"
     gene            complement(9311..9931)
                     /locus_tag="PLT31_03305"
     CDS             complement(9311..9931)
                     /locus_tag="PLT31_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiopurine S-methyltransferase"
                     /protein_id="HPW94195.1"
                     /translation="MLTQNLPDFWDSLYSEGKDYWNFKKATPALLKFFEHSSCPKTGS
                     VLVPGAAFGYDAEQWAIKGHDVLAVDFSPTAVDELDRLSRKYKNLKSLDLDLFDLSPK
                     DPKKGGQLFDIIYEYGTFSAIHPGRRDEYFEVWYKMLKDDGIIISLFYPLVNGNTFQG
                     PPHCTSEGELMARLGGIFDVIEKIPAQNSLPDRVGKEEFWILKKII"
     gene            complement(9925..11001)
                     /gene="rlmN"
                     /locus_tag="PLT31_03310"
     CDS             complement(9925..11001)
                     /gene="rlmN"
                     /locus_tag="PLT31_03310"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPW94196.1"
                     /translation="MNGLHNIKSLTLNELKTWLKSVGEKPFRAEQIQNWLFRNQVENI
                     EEMVNISPSLQSKMKEAFYIHSLTEELKKESVDGTIKWLFKTHDNHYIETVMIPTNGR
                     FSVCVSTQIGCAMNCSFCRTAKMGFIRNLEAGEILEEIIRVNWHLKKGQVLNDEGAAA
                     EVTNIIFMGMGEPLNNLEHVHRTCCTLHNQKLFNMGRKRMTISTSGVVPKIKELVDRN
                     TPCCLAVSLNSTNNENRSAVMPVNRTWPIEKLLEATDEYTRRTDNYVTFEFVLIKGVT
                     CTKEAAKELIRICAPRRCKVNAIALNDGDDPHLEAPTTEEIEQFLEIVRSAKVQITIR
                     NPRGRDILAACGQLAYKQGTAEIC"
     gene            complement(11267..>12642)
                     /locus_tag="PLT31_03315"
     CDS             complement(11267..>12642)
                     /locus_tag="PLT31_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPW94197.1"
                     /translation="EIQAFAEQAAVKQIESVRKRIKRRSIRRLGLAKKYEMKEDSVLV
                     HFDSVYTLFKKDYLSKDTVSDLTKRRFPDSLLYHHAEVAAILIENETGAIRAMVGGSN
                     FNESKWNRTVQSLRQPGSSFKPFVYATAMDNGASPCDSVNDQPVTIPDPDDPSKTWRP
                     ANFGHDFEGMMTLRRALYLSKNLPAILTGMKYGLNNVVNYSRKFGIQKAPLLAVPSLA
                     LGSVGATLMEMTSAYTVFPNGGNRIEPYMIESIVDRSGEVIEKHFKVEHEVLRAPSAY
                     IMVDMLKDVNVRGTAARVWSNGFYHPSGGKTGTTNDYTDAWYIGFTKQYTMGVWIGPD
                     APGTMGAGHTGTEDALPIWIDVMKELHKDLPKKAFPMPAGVISKSVCNFTGKIAGEFC
                     GTKTYCLYSSGYVPEEICDGNHYDLKVKSADNATLFSSKGSDVAPPSSPKSKDQPVKP
                     NRTRKMF"
BASE COUNT         3741 a         2449 c         2202 g         4250 t
ORIGIN      
        1 ataagcgtca tcaatcattt cagcaaaagt attgtaccat tctgcaaaga tttcgacttc
       61 aacatgatcg acaccaggaa tggtcaaacg tacgccacag ctttcaccaa tacgatcttg
      121 gtaattagca acttcaggac gtaaaagact taaatctaga ccgtccaaat cttctggatc
      181 aatccaaacg gcttcatctt cttctttgtc cgattcttct gtgcattcac attcacattc
      241 ttcgtcatca cattcacagt catcacattc acagtcatct tcacagtcgc agtcgcaatc
      301 gcaatcgcaa tcatcacagc agcagcaatc gtattcgtca tcttcttcat caatgccgct
      361 atcataatag aactcatctt cttcaagata aagagtttct acaaaaatgc cttttgcatt
      421 taatgctttt gctgcttcga ggaattcttc aaatgtccct gcaaaatagc gagaggcttc
      481 agcatcttca tttaatgttt gtacaggaat tggtaaaaaa ccatgtttct gtatattttc
      541 gcaaatttcg tcactaaccg ttttttttat ttcttcgctc atggtttatc tccgttgtta
      601 tttatgatac tttaataaag aaggtgcttt ttcatgtatt aatttgagaa tacgtgcttc
      661 agcttcatct cctcttgccg tttgccttac gctaactaga gggagaaata gagggctatt
      721 aaacaatgtt cctaaaggaa tagggttttt acgacagaaa gtaatttcta taaaatcacg
      781 agctgcatct tgtaagtcgt ctgccttttc aaaatgacca tctaaaaaag ccctgtataa
      841 ctgaacgaaa atagaggatg cttcagaaat attagcagaa gcgctgatta ggccctttcc
      901 acccatttct agtatatcaa taaacgctcc gtcttcgcca ctgagaactg caaaatcatt
      961 gtttgctgtt tctttaatga ctcgaacagt gtcttcgtga tacttttcac caattctaaa
     1021 ttctactgct tgttttaatc caatgatgtt tttgtcttca gataacttaa tgagtgtatc
     1081 tggatgaatg taattggatg ttcttcctgg gacgttataa agaataattt ttgcgcccgt
     1141 ttctgaactt aaagttttaa aatgtttttc aatcccttct tgtgaaggat tgttgtaata
     1201 gcctgtaacg caaagtactg gaacagaagc tatttttaaa atttcttgaa tcatttcaat
     1261 ggattctcta gtgcagtttg atcctgcgcc agcaatcact ggaactcttc cattcacata
     1321 atccaacgtg aatttgataa tatcaagatg ttgtttgtgt gttacagtcg cactttgacc
     1381 tgtggtacct acaggaacaa ttccgctcac accagattca attaaatcat cgatcataag
     1441 agccattttc ttataatcaa ttgtattata aagatttttt gggtcgtcac tcatgagtgg
     1501 agtgaacaaa gcaggaaata caccagttaa ttgagaagat tttgttattt gcatgatgta
     1561 aaaatagaca tttctaatca acttggaaat acaaatttct aaattaccat tttaaacact
     1621 ctcccttttt agaaaggaat cttttatgca aacgattact caattatgga aaaaaattca
     1681 atcacctgat tttaatcctt caacagattt tgctcttgta gaagaaatta aaaaagaagc
     1741 tttaacttca caaaagccaa caaaagtcac gtttggaact tctggatggc gtggagaaat
     1801 tggatctgaa tttactatcc gcaatttaca agtcgttgga gcggctattg ttcgtttgta
     1861 tcgcgaagcc actcctgaat tattgaagtc tttaggtgtt cttaattttg aagatcttca
     1921 aaaacgaggt cttgtcattg ggcatgataa tcgtttgctt ggaaaagaat tttgtatggc
     1981 agtggcatca caatttcaaa aagctggggt caagatttat tatggtggag aaatggctac
     2041 accagaattt tctgcttctg ttgaactttt gggggctgca tgttctatta atatgactcc
     2101 aagtcataat ccaagccatt acaatggaat caagtttaat cctgcagatg gtggtcctgc
     2161 tggtccagaa attacagatg tcattacagc tctatcaaat gaaatgatga acactcatca
     2221 atttgaacca gtgggcgagg tttcatttga aaagattgat ccagttaaga tttataaaga
     2281 atttttagtg aaacaagaaa ctatcaagtt tgggcgtatt caaaaattaa ttgatgaagg
     2341 tcgcttaact cttgtttgtg atcacgttca tggctctact agagggcgtc cagcggcatt
     2401 gcttggaaat ccagaatgct taatcagtct tcgtactgaa gatgattccc tttttggtgg
     2461 tgttgctcca gaaccaagtt ctaaaaatct agaacttgta cgtcaggctt tagatgcaag
     2521 caagaactgg ttccgtttag gtgccatttt tgaccctgat ggagaccgta ttcgttttta
     2581 tgatggcact cgcgaaattg atatgaatag ctttggtgcg atagctttcc attatatgat
     2641 cacatggcgt aaggaacaag gggttgtagc gaagtctgtt gcaacgtcaa attttgtcaa
     2701 tattattgcc gaaaaattag gcgttcctgt gatggaaacc cctgtgggct ttaaaaattt
     2761 cagaccttgg ttggctagaa atgcgcgccc taaagcctta attgcttttg aagaatctga
     2821 cggtatttct ggattaaaca acactcttga aaaagacgct cagtttggtc ttcttcttgc
     2881 actagaaatt atggccgtta caggtaaaaa tctaggtgaa tacttggatg ctctttatgc
     2941 tgagtatggt cgtttttatc caactcgttc tggttttgaa gtcgataagt ctttagttgg
     3001 tgatccactt attgctaaag taaatgctgt tgcagaaaga gctcaaccta aaatgaaagt
     3061 attagtcggt tcagaagaaa aagaagtcaa acaattatta actctagatg gtgttaagat
     3121 tattttcact gatgattctt ggatgctcat tcgtccttca ggtacagaac ctaaggttag
     3181 aatttataca gaatgtcgta aaccagaaga aaaggatgct ctttttgaag ccgctaaagc
     3241 tttatttttt aaagagtaaa aggatattat gaaaaagata attttattcc tattctgctt
     3301 attaaccaca atatctgctc aagtagagcc tgaatttcag ggttttgctg gtaatttatt
     3361 gaggctaaaa aaagcaaata aaggctatca tgatttttcg atggaaataa cagttcctcc
     3421 ttgggctttt tctgtcgaag gttctgtaaa gtctcctggt ggcgatccag atgttttgtt
     3481 taatggcatt tttgatgaag aactcattgt ggtaatggca tatattcctt acccaacaca
     3541 aggaaaagat ggtaatgaat atcaaacagg catgtttgat ctcatgatct atctacaaga
     3601 tgaaaaaact gtgattaaag atctttcctt taaattgctt tctcctgcaa atgatagctg
     3661 ggcaaaagaa tcatttgaaa tggctaaaag ttctagtcaa atgctaggcc ctatctggaa
     3721 tggaaaattt gagaaaaaga ttattgtcgc aagtgccact ccattaacaa agaaaaaaat
     3781 aaaagctcta caaaagaaaa tgaataagaa atgaacgtat tctttttcta ttaattttaa
     3841 ggtattatta aaaaagaaac ccactaaatt gatagtgggt tctttttatt tcttcaaaat
     3901 caaaagctaa tctcgctcct aaaaaataaa ccttctggcg aaaatgaaaa accaccagct
     3961 tggggccagt ggttatcttt tttattatgg ggtataataa ttattcttcc atatcttcta
     4021 catctatttc agcattatga taaacgtctt gaacatcatc attgtcttcg aatttatcaa
     4081 ttagttttaa tagtttttga gcatcagcac gttccaattt aatggtatca tttggaacat
     4141 aagctaattc agcgctcatc atttcaatat tagctgcttc taaggcttta gttacggctt
     4201 caaaagcatc aggagttgtg cttacttcat gcacattttc ttctgtagac atgtcactag
     4261 cacctgcttc aagaacaatg tccatgactt tatcttcagt gtacttttct gcatcaatta
     4321 aaatcacgcc cttgtatgtg aaggcccatg cgacagagcc agattcaccc atgttgccgc
     4381 catttttatt aaaaacatta cgtacttcag caaccgttct tactttgtta tcagtcatgc
     4441 aactgactaa aatagcgacg ccacctggac cacgtccttc atacattggt tcggtcatgt
     4501 cggcaccaga gtttccgcca actgctttct gtatagcact ctcaatgttt ttagctggta
     4561 aactttgacc tttagcttta agaacggcgg ctctcaatct tggattggca tccaaattat
     4621 caccgcctaa ttttgcagca attgagattt ctttaattaa tttattccac gctttggcac
     4681 ggtcaacatc tgtttttgct tttttacgtt tgatggttgc ccatttggaa tgtccagaca
     4741 tatttacctc aaagatctaa agttcaatag ttaatgttaa aaataaaatt ttatcgttaa
     4801 tgttgtagag tctttctata atcatttaca gcttttttgt attctatgat tgatttagtg
     4861 attctatttg ctaaatcttc ttgccctttt gctgaattca tataattttc atctttagga
     4921 tgggtgataa agcctaattc tacgagaaca gcaggcatta gtgccccgac taaaaccata
     4981 aatccagcac cgccaacgcc tgtaccaagt tgtttgattt tgccattatt gttaaaactg
     5041 tttaataatt cctcagtgta catgtagctg ttttgtttgt atttttctaa acgagcttct
     5101 aatttaaacc actccaaagg ggataactct tcttttgctt ctttatcccc ataaagagag
     5161 gccaccttgt tttctcgacg agcaatagct ttatcttctt cgctctctgg agctctaaga
     5221 acatagaaat gataaccttc tgtacgcttt tgcctttcaa taccatcaac ggcattacaa
     5281 tgaagactga taaataaatc gccatcccat ttgtttgcca aattagctct ttgtgaaagt
     5341 tcaataaaca cgtctttttc tcgagtgagc ttaacgttaa agccttcttt agtaagtttc
     5401 ttttttagca atttagctac agataaaaca atgtctttct cttgactttt tttccctaat
     5461 gcacctggat ctttgcctcc gtgaccagga tctattacta tggttttaac ttcgcgtgtg
     5521 ccagcagttt cttttttaga ggtttggggg attataggct taggcttggc tttcggtaaa
     5581 tcagtaaata ggactaaatt ttttatggta tctaaggtga tatttttatt gaattctttt
     5641 tgaaaaaact taacggtttc ttctgcaggc gcaaaaactt tatctttttc aaggtataca
     5701 ggagctgata atgattctgt ttttccatcg tgtaaaaaat aaggaaatcc aacgacaaaa
     5761 acccattcag aagaattccc tttaagagag agtctttttt ccactggatg aaattctata
     5821 gtgtaatttt catgattagc gagagcttct aggtttagca tccaaataga attatgcttt
     5881 ataagcatag gactagcagt agcagagtat acaaagactg ctaataaaaa taacaatata
     5941 ttttttagcg atgcattttg ataattcgag ccatttctag cttggcgtct cgcgttataa
     6001 tatcttgtct tttgtcttgt tgatctttgc ctcgacatat tcctatttca agttttgcgt
     6061 aacgattttt gaaatacaat tttaaaggaa caattgtttt tccttttgtt tcaaccgctt
     6121 ttctcatttt ttgaatttca tgaaggtggg cgagtagctt acgccgacga gcggggatat
     6181 gattaaagcg attggcaaat aaatactcat caatatgagc acccactagc caaatctcat
     6241 ctttatctga agatacatca acccaagcat cacctagatt acatttccca tcacgaatag
     6301 attttatttc tgaaccaatt agcataattc cgacttcaaa acgctcttcc acagaataaa
     6361 ggtggtttgc tttacgattg atgatagtcg gtgttttttg ttctttaggc ttgttcattt
     6421 tttgattcta tggattcaga aaaaagctca tctatgactg gagtatcgtt taagaatttt
     6481 tttaattctc ggctggcttc aaaaacaggt ttaatacctg ctttaacaat cacatcctct
     6541 cctgtttttg gatttcttgc ttttcgagct tttctctctt taattttaaa tcgaccaaat
     6601 cctcgaattt caatattatt cccttcactc atggctttta caacagatga aagaaatgat
     6661 tcaataatca ctttggtatc tacttgagtt agtccagttc gtaaagcgat atcttctaca
     6721 atatcttttt tggtaatgtt actgttgttt gtcgccattt tattgtaact ccaaaaaaat
     6781 caatcactta taaatataag ttataatttt tttagacgtt agtcaatgag aatattacca
     6841 acctttcgat cgtttttgct tgttaggcac catttcatct aaggctattt ttaattcttt
     6901 gcaaccttct ccagtaattg ctgaagtaag aattactttt tctttcttct ttaaaaagat
     6961 tttttgagtt ttttcgattc ctaaatccga cttattcaaa gcaattaaat agggtttttc
     7021 tgctaatttt ggatgaaaac caagcaattc ttttttaaga attttgaact tttcataagc
     7081 atcttcttca aaaccatcga tcacaaataa caacgtatgt gttctttcaa tatgctttaa
     7141 aaactgatga ccaagacccc ttccttcact agctccttca attaaaccag gaatatcagc
     7201 aacaacaaag ctacgagagc ccaattgcac aattcctaaa ataggctcta gagtggtgaa
     7261 tggataatct ccaattttag gacgagcact agatatttta tgaactaaag aagatttgcc
     7321 tgcatttgga aagcctacaa gcccaacatc tgccatgagc ttcaattcta gataaagcat
     7381 gcgcttttct ccaggtttcc ctggaataat ctttctcgga gcttgattac taggagttgc
     7441 aaagtgctgg tttccaaagc ctcctcgacc tccttttgca gcaacccaag tttgaccaat
     7501 ttctgttaaa tcagctaaaa ttcggccttc ttcgtcttta actaatgttc cccttggtac
     7561 attcactatt aaatcatcgg cactggctcc agtacaacga gatgctccac caggggaacc
     7621 acttttggcc ttgtacaggc gaatatttcc catatctaag agagtggagt attgttcatt
     7681 gacggctaga atgacactgc ctccacgacc accatcacca ccatcaggcc cacctaatgg
     7741 tgtgtatatt tcgtggcgaa aactacataa accgtctccg cctttgcctg atcgcacttc
     7801 tattactttt tcatctaaaa acattataga ctgattcctt catctagccc aagagctata
     7861 ttcatgtttt gcactgcggc accactagct cctttgccta aattgtcgat gattgtggtg
     7921 acttgcatca ggttctcatt tccaaaaatc tgaatccgtg ctttattagt gtcgttacat
     7981 acagtacaat ctaaacgtcc gtgatgaagt tcagggattt tttcgtaagg catgatttca
     8041 ataaaatgac acccatcata atgagattga aatacttctg taaggctttc aggcgttatt
     8101 ttatgagtga attgtgatgc aaaaagatta atggaaacag ccattccttt atagaaaggg
     8161 cctaaaatag gactaaataa aggaggattt tttaattccg tatatttttg catctcaggc
     8221 aaatgcttgt gagtaagagc gagtgcgtaa gggcttgggg caaaaataaa tgattcttta
     8281 ccagcaactt ttgcattatt aatctctgtt tcatattctg aaataagctt ttttccacca
     8341 ccagaatacc ctgtgattcc ataagcagaa acaggtgttt ctagaggcaa aagccctttt
     8401 gatgtcaaag gatagagtcc caaattaaat gctgtagcat ggcatcctgg atttgcaatc
     8461 ctatgacttt gacgtatttg atctcgaaat ttttgggaaa gttctggcaa tccgtaggcc
     8521 caaagtggat tagtacgatg agcagtagag gcgtcgataa tttttgtctt agaattagtg
     8581 caaagaagag cgctttcttt agcggcttca tctggtaagc ataaaaaggt gatatcagaa
     8641 gcattaatga gttttgctcg agcttctgga tcttttctaa gcgcagaatc aatttctaat
     8701 atttcaatat catcacgatt gtttaatctt tcatgaatct gtaaaccagt agttccttct
     8761 tgaccatcga caaaaacttt aattttcatt attagcccta aactttattg accacagcac
     8821 tttttatatt ttttcccaga accgcaaggg catgggtcat tacgccctac atctgggcct
     8881 tcttttttta ccgtttcagg ctttacagcg cggccgtcat aaaagaacca ttcgtcatta
     8941 attttatgga attcaccaag ttcatgatga ttgcgagtaa tgttaccttg cttaaagcga
     9001 gcaataaact caacccaacc gatatttttt tcttcttctg tctttgtatt gcgaatttca
     9061 ataccaaccc attctgattc acgactccaa gcttcgacac ttttctcatc aaactcttct
     9121 ctttgagttg gatctaaaga atcttttaac caagatattt cgtgttttgc ataagctgta
     9181 taacgagaac gcattagaac ttctggtgaa gaagcctttt ctgctttttt tataataggt
     9241 tcacaacatt cgctatattt tttaagtgac ccacaagggc ataaatcttg agacatatga
     9301 ttacctgttt ttaaatgatt ttttttagaa tccagaattc ttcttttcca actcgatctg
     9361 gtaagctgtt ttgagcgggg attttttcaa tcacatcaaa gataccacca agacgagcca
     9421 ttaattcacc ttcagaagtg caatgaggag gaccttggaa ggtatttcca ttcactaatg
     9481 ggtaaaataa agaaataata atcccatcgt ctttgagcat tttgtaccaa acttcaaaat
     9541 actcatctcg acgccctggg tgaattgcag aaaaagtgcc atattcgtat ataatatcaa
     9601 ataactggcc acctttttta gggtcttttg gggacaaatc aaaaagatct aaatcaagag
     9661 attttagatt tttgtattta cggctcaagc gatctagttc atctacagcg gttggactaa
     9721 aatcaacagc aagaacatcg tgccctttga tggcccattg ttcagcatca tagccaaatg
     9781 cagcaccagg aactagaaca cttccagttt taggacatga agagtgttca aaaaacttta
     9841 aaagagctgg agtagccttt ttaaaattcc aataatcttt gccctctgaa tagagagagt
     9901 cccaaaaatc gggtaggttt tgtgttaaca tatttctgcc gtcccttgtt tgtatgcgag
     9961 ttgtccacac gctgctaaaa tatctcttcc acgcggattt cttatggtaa tttgaacttt
    10021 agcagagcga actatttcta aaaactgttc aatttcttct gtagtaggag cttctagatg
    10081 tgggtcatca ccatcattta aagcgattgc gttgacttta caacgacgtg gtgcgcaaat
    10141 acgaatcaat tcctttgcag cttctttagt gcaagtgact cctttaatca aaacaaattc
    10201 aaatgtcaca taattatctg tgcgtcttgt atattcgtca gttgcttcta aaagtttttc
    10261 tataggccat gttctattca ctggcattac agcggagcga ttttcgttat tggtactatt
    10321 taagctaaca gctaaacaac acggagtatt gcggtcgact aattccttta ttttaggaac
    10381 cactccagag gtgctaatgg tcatacgctt acggcccata ttaaataatt tttgattatg
    10441 caacgtacaa caagtacgat gtacatgttc aagattattc aatggttcgc ccatacccat
    10501 gaaaataata ttggtgactt ccgctgcagc tccttcgtca tttaagactt gacctttttt
    10561 caaatgccag ttcactcgaa tgatctcttc taaaatttcc ccagcctcaa gatttctaat
    10621 aaagcccatt ttagctgttc tgcaaaaaga acagttcatt gcacagccga tttgagtgga
    10681 aacacaaact gaaaaacgac cattggttgg aatcatcact gtttcaatgt agtgattatc
    10741 atgagtttta aataaccatt taatggtacc atccacggat tcttttttta attcttcagt
    10801 taaggaatga atataaaacg cctctttcat tttggattgg agagaggggg agatattgac
    10861 catttcttcg atattttcca cttgattacg aaacaaccaa ttttgaattt gttccgccct
    10921 aaaaggtttt tctcctacac tttttaacca agtttttaat tcgttaagag tcaatgattt
    10981 tatattatgc aagccgttca ctagacggta aagatagtaa aatatacgta ttttgtatag
    11041 atttgagaaa aatgaaaacc ctccagaagg ctgcttttag tagtaaaaat cgttaaaagt
    11101 ctcattaaaa aactcttttt actagcctta ttggcctgtt atatcaaaag ccaacttgcg
    11161 aatagaccat ttgacaatca tagagataaa aaagttttcc tattatggct aggaatactc
    11221 agtgatttaa ggccagttat gattttatta tggcttcaaa agataattaa aacattttac
    11281 gtgtacgatt tggttttaca ggctgatctt tgcttttggg gcttgaagga ggagctacat
    11341 ctgagccctt actactaaag agagtggcat tatctgctga ttttactttc agatcataat
    11401 gattaccgtc gcaaatttct tctggaacat atccagaaga gtataagcaa taggtcttag
    11461 taccgcaaaa ttctccagca attttacctg tgaaattaca tacactttta ctgatcactc
    11521 ctgcaggcat agggaatgct tttttaggca aatctttatg taattccttc atgacatcaa
    11581 tccatatagg aagagcatct tcagtacctg tgtgaccagc tcccattgta cctggtgcat
    11641 caggtccgat ccaaacaccc atagtatatt gtttggtaaa acctatatac caagcgtctg
    11701 tataatcatt tgtggtacct gtttttccac cactcgggtg ataaaatcca ttagaccaaa
    11761 ctcgggccgc tgtacctcgg acattcacat ctttaagcat atccaccatg atgtaagcag
    11821 atggagctct taaaacttca tgttcaacct taaaatgctt ttcaattact tcgccgcttc
    11881 gatcgacaat gctttcgatc atgtagggct caattcgatt tcctccattt ggaaatacag
    11941 tataagcaga tgtcatttcc atgagcgtag cacctacaga gcctaaagca aggctaggga
    12001 ctgctaataa aggagctttt tgaattccaa atttccgaga ataattgact acgttattca
    12061 aaccgtattt catgcctgtt aaaatagcag gaagattctt agataaatag agagctcttc
    12121 ttaaagtcat cattccttca aaatcatgcc caaaattcgc agggcgccaa gttttagaag
    12181 gatcatctgg gtctggaatg gtcactggtt ggtcgtttac agaatcacaa ggacttgctc
    12241 cgttatccat cgccgttgca taaacaaagg gcttaaaaga ggatccaggt tgacgtaaag
    12301 attgaaccgt tctattccat ttagactcat tgaaattact accaccaacc atagcacgta
    12361 tagctccagt ttcgttttca ataagtatgg cagcgacttc agcgtgatga tataaaagac
    12421 tatcaggaaa gcgtctctta gttaaatcgc tcacggtatc ttttgaaaga taatctttct
    12481 taaatagggt ataaacgcta tcaaaatgaa caagaacgct gtcttctttc atttcgtact
    12541 ttttagcaag accaagccgt ctaatgctgc gtcttttaat tcgcttgcgt acactttcaa
    12601 tttgtttaac agcagcttgt tctgcaaatg cttgaatttc tg
//
LOCUS       DAOOBB010000042        12302 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_6599_length_12302_cov_4.621540, whole genome shotgun sequence.
ACCESSION   DAOOBB010000042 DAOOBB010000000
VERSION     DAOOBB010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 12302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12302
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6599_length_12302_cov_4.621540"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="PLT31_03320"
     CDS             <1..677
                     /locus_tag="PLT31_03320"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HPW94198.1"
                     /translation="VIPARMGSSRFPGKPLAKINGTEMIIHTLNRAHLAGCFDSIHCA
                     TDSEVIASLVRSHGYDAVMTGVHATGSDRVMEASEKLGLDLVLNLQGDEPLVDLDLLR
                     NVSNAIQMEPSSWVTAASPLDPNDAYNRNIVKILVEGDYARDFQREVPLDKVMLWNCH
                     RGIYAYGKEARDEFSHLPLGEEEKKRSIEPLRIMNLRPIRVVFSEKASVSVDVPADIL
                     VVEQMI"
     gene            707..898
                     /locus_tag="PLT31_03325"
     CDS             707..898
                     /locus_tag="PLT31_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94199.1"
                     /translation="MKQTESLYTYNDKTTFIKFTGDDLSRQEMKGATMRLSSHELNFI
                     LLNRSPLFVELWRSTDEQI"
     gene            885..1307
                     /locus_tag="PLT31_03330"
     CDS             885..1307
                     /locus_tag="PLT31_03330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPW94200.1"
                     /translation="MNKSEKKALLLFLVLFGIGFLIRLLPIEPVPAIEEFSVEEKESL
                     LEGEVKDVKESVVENKKVSFPVKKTKKKAEIKFPISINKATLEELCALKGVGPKLAEK
                     MIAYRDSVGGFKTASDLLKVPGIGKKKSKNILHDVIFD"
     gene            1353..2264
                     /locus_tag="PLT31_03335"
     CDS             1353..2264
                     /locus_tag="PLT31_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94201.1"
                     /translation="MKLQLGIEAGDAALKLSLFDPQAKKVLKTSYLPTETSPLDDIYT
                     FETTLQSWLQENDLESIKSIALTVSVFRGLVRQVYIPPEASSSIRDYLSWTLSLMTNI
                     DPDVYYFDYHTLNGDASLGYTVLLIAVRRVWVDAVRKGFRSKKIAPTIFELDVVSLYN
                     LLEYDGALEKGTECLIKADFSGVTIMWMAKDDLKALRCVSTLELVDKSKEEAYLLLSK
                     QVLEQLQLADEENGVSVKRIRLCGELANDITFVNILREQLNGYTLTLLDSFANIRLPV
                     DDESTACVLNCVGAIGASLRSVGGGNP"
     gene            2261..3280
                     /locus_tag="PLT31_03340"
     CDS             2261..3280
                     /locus_tag="PLT31_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94202.1"
                     /translation="MIQLNLIEAASQTASLEAKVQVKLNTKNKTTLKSHKILIAFGSV
                     AAVLVLFAGSLILFGLPKPLHGVIPEAVLSTLGVEDPSREALLQGRGKAQMTTAGGLL
                     EKQREAERAALLEKQSISVENIVQGVSPKMFNQTVAKTHYSNYLPLEQIAYQNAALSQ
                     LLVFINTATPDNISFSDLIFEAPNYYYVRGVAENPSLQRSFLERIKAVSSSFKTPTLP
                     ENAPVTDITAFGEYNVAQPNLNAHKSLVSADGVAEELKHFAALDVLKKIKFSGLDKPK
                     IEDYGVFKKYSFKASTNIDYISLMNFIVELQKSPVRLGVQKLEMVPGKKNLAATLHLV
                     MYVAP"
     gene            3284..3817
                     /gene="rsmD"
                     /locus_tag="PLT31_03345"
     CDS             3284..3817
                     /gene="rsmD"
                     /locus_tag="PLT31_03345"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HPW94203.1"
                     /translation="MPIRVTGGDFGGRLIPSPLSSKTRPTASMTREALFNILFDVSDY
                     TVLDLFAGSGIVGIEALSRGAKCVYAVEQAHRQAHEIYKAYQSLGLEKSLQLLEKNAL
                     SLTQGNLLVEGGFDLIYADPPFTEEYPDLLPFLKWLSPQGTAVFECPSRSIPKWAKNA
                     KVKRYGESTLLFFKSSL"
     gene            3841..4335
                     /gene="coaD"
                     /locus_tag="PLT31_03350"
     CDS             3841..4335
                     /gene="coaD"
                     /locus_tag="PLT31_03350"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HPW94204.1"
                     /translation="MNIAVFAGSFDPLTIGHYDIVTRATSFFDEVIVLVNSNVSKHSL
                     FSVDARVRMAQAAFSSMDCIRVESFDGLTVSFLDRVGARFLIRGIRHAQDLEYERALS
                     WNNQKLNSKIETIFLLSAPEHMAVSSSVVRELLSYNQNVDTYVPENMREILFSEWRKL
                     NVRS"
     gene            4322..5149
                     /locus_tag="PLT31_03355"
     CDS             4322..5149
                     /locus_tag="PLT31_03355"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPW94205.1"
                     /translation="MFGLKPFGFIAKPVRVLISINAIVFGLVILGALIGLDSQALVLS
                     VGALIPFEFTQAWRFLTYNFVHVSFMHFLFNMLMLWMFGDEVAEFMGHRKFVGLYIFS
                     GIFAGVFSIPFYLFGSLSPYAMIIGASGALMGVFVAYYKFFPERTLLLFFVIPLKMKY
                     AIWVLIALDIMMSQTNDSIAHFTHLGGVLAGFIFMNFFYRIPFWEKWFQPKRKKQHSQ
                     HNSSEKVLEGEVGYLDQDKQMDAILSKVNKEGVNSLTESERQYLLKAGERLRRRRGL"
     gene            5188..6150
                     /locus_tag="PLT31_03360"
     CDS             5188..6150
                     /locus_tag="PLT31_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HPW94206.1"
                     /translation="MKKILGMGAALVDVVYTVEDAWVKASGSEKGGMTLIDQKKSQAW
                     LAEVKNPVLVPGGSACNTIVGLSKLGARAAFIAKIGHDELGKRFETHLSDSHVESKLL
                     FSDKPTGSVLAAVTPDAQRTMFTYLGASDDLDVDDIQDSLFQDVSLFYIEGYRAYNGP
                     CFKKALQLAKKNGVTTVVDFGAFSVVNDCRALFDNLFQEKLVDVIIANEDEALAYTGK
                     TEEEALAVLASKSSVAVVKLGKRGALISKDGKVTSVDAEQVNAIDTTGAGDLWAAGFL
                     FAYLEGESLSVCGRLASLVASEVVQVMGPQIPEEGWFRIKHFFR"
     gene            complement(6240..7103)
                     /locus_tag="PLT31_03365"
     CDS             complement(6240..7103)
                     /locus_tag="PLT31_03365"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPW94207.1"
                     /translation="MSDSFLKEAWSDHSRLWEKNTWVYPVISRRAKGLSIGVNLNPDK
                     RCSFDCVYCQVDRSIPGSNKRLDIEAMTKEIELIIQEYEKNGLTSFSNFKLIEESKRQ
                     IKDICLSGDGESTLIKEFPAVCERLLEIQHKYSKYNLKLTLITNSTRLDQEYVREGLR
                     YLTQTNGEIWAKLDAGTEEWFQKVNISPYPLEVIQRNIELTVKDFPTRIQTMWCEVDG
                     ISPSPEEVRKYIERVKKIYHHHSKNLLEVQLYSVVRNTARPHVLPVSKSFMEDVANQL
                     QSQIDIPVGIY"
     gene            complement(7096..7239)
                     /locus_tag="PLT31_03370"
     CDS             complement(7096..7239)
                     /locus_tag="PLT31_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94208.1"
                     /translation="MKEKETYRELEIGELPCPVENCRGVLKVEPDNSYKLTCDLCGYI
                     KDE"
     gene            complement(7236..7967)
                     /locus_tag="PLT31_03375"
     CDS             complement(7236..7967)
                     /locus_tag="PLT31_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HPW94209.1"
                     /translation="MKRLVFTLLILLACTLFAEEEPRHYDYNGASRQMHRIYYGELQE
                     TLYCGCSYKNKKSIDFNSCDYAPRENPKRKSFRRAHRIEWEHIVTAHNMGHFLPCWKE
                     GGRKNCSNNNEQFDLMEGDLHNLYPAIGEVNGDRSNFMFSQWTNEPEPMYGKCSTIVD
                     FELKKVQPRKEARGIIARVSFYMEKTYGVSLSNQDRKLFEAWDRMYPVNSKECLRDKR
                     IYNVQGNHNPFVYDKCLEQKLYLDE"
     gene            complement(8007..8990)
                     /locus_tag="PLT31_03380"
     CDS             complement(8007..8990)
                     /locus_tag="PLT31_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPW94210.1"
                     /translation="MTYSPASNRYDSMIYRRCGKSGLKLPAVSLGFWHNFGGVDLFEN
                     SREKVRYAFDHGVCHFDLANNYGPPAGSAEETFGQILKKDFLSYRDEMTISTKAGWGM
                     WPGPYGDFGSRKYLIASLDQSLKRMGLDYVDIFYHHRPDPETPLEETMGALDHIVKSG
                     RALYVGISSYDGPSTQAAYDILKSMGTPCLIHQPQYHMFNRSLEGNLQSVLNKNGIGC
                     ITFSPLAQGLLTDRYLNGIPSDSRAAKSPFLTKDQVESSLSKVKQLNELAKKRGQKLS
                     QLALAFNLRLPEVTSVLIGASRTEQIQDALGALQNLSFTSEELQLIDSILA"
     gene            9135..10397
                     /locus_tag="PLT31_03385"
     CDS             9135..10397
                     /locus_tag="PLT31_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPW94211.1"
                     /translation="MKKSGMDFLNDSLLYGMKPGLQNMETMCRYFNHPEKSFQVIHVV
                     GTNGKGSSAYYLAQILKAHEKKAGLFSSPHLVSLQERIRVNQEAISKADLESTLLEVQ
                     KAAIEKKISPTFFEIMTMVCLLYFREQQIEVAVLEAGLGGRLDSTAIAKGKYCVLTSI
                     GLDHTEILGDSLSSILNEKLGILSAGSLLIRFSLGSNLDTQASQLVKEKYASEMVVER
                     DLSLVLLNGGTHFKENATLSLKAAELVLGKEWKNSVALEALSQNAWAGRMHLLKNKNE
                     KLEFILDGAHNLHAMERLVETLNADFHGMTFPCVFGAVKDKDASDMIRLLAPFVSKWY
                     FTRTPYMRFRETEELAEILLQYGKTPERQFMLSKEDLLECQKEANGAPILVTGSLYLI
                     GGVVDLLKNDFESLSFFRGLEATTNEHR"
     gene            complement(10400..>10676)
                     /locus_tag="PLT31_03390"
     CDS             complement(10400..>10676)
                     /locus_tag="PLT31_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPW94212.1"
                     /translation="SIPQILSGNSSTYAQYTLRSSNRKQMESILKECGIPYCIHYPKA
                     LRKQPCFLGLSNPENPSTDLVCKQVFSLPVCAFTDVSEVLQKLKTFF"
     assembly_gap    10677..10686
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10687..11473)
                     /locus_tag="PLT31_03395"
     CDS             complement(<10687..11473)
                     /locus_tag="PLT31_03395"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPW94213.1"
                     /translation="MIPFVNLSAQYKAHETELKSAIDRVLYSGAYIGGPDVSALEEEL
                     AHYVQVPYAITCASGTDALFLALEALGLQPGDEVIVPDFSFIAPAEAVRSLGGVPVFC
                     DINPNTFLIDPSSVESLITSKTKGIIAVDLFGQCAPYEALSKISQKYHLWILEDAAQS
                     FGASQNGKKAGSFGNMAITSFYPTKPLGCFGDGGALFTYSEKLADRIRSLANHGSSIR
                     YYHELIGRNSRLDSIQAATLRIKLKYLDNELSIRRENALQYNSF"
     gene            11892..>12302
                     /locus_tag="PLT31_03400"
     CDS             11892..>12302
                     /locus_tag="PLT31_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94214.1"
                     /translation="MERLNFVGIMEGSPQSLDKYSLVYYIDEQQVNDVYKTRRLSFNS
                     INEESNIGRLELYGAGGYCNVYMKNGQFANCLHPLRGQFFVKVFLRHNETGDSVLVGK
                     HFIDMDGYLLTSVRTEVYLGPNKEEYEPRNCYNFD"
BASE COUNT         3729 a         2088 c         2527 g         3948 t
ORIGIN      
        1 gtgtcattcc tgcgcgtatg gggtcttcgc ggtttcctgg caaaccacta gcaaagatta
       61 atggaacgga aatgattata cacacgctaa atcgagctca tttagcaggg tgttttgatt
      121 ccattcattg tgcaacggat tcagaagtaa tcgcttctct agttcgttct catggctatg
      181 acgctgtgat gacaggtgtt catgcgacgg gatccgatcg cgtcatggaa gcgtcagaaa
      241 aattaggcct agatttagtt ttgaatttac agggagatga acctcttgtg gatttagatc
      301 ttttgagaaa tgtatctaat gccatacaaa tggagccctc ttcttgggtc acagcggcct
      361 ctccactaga tccaaatgac gcttataaca gaaatatagt gaaaattctt gtcgagggcg
      421 attatgctcg tgattttcaa agagaagtgc ctttagataa agtaatgctt tggaattgtc
      481 atcgaggtat atatgcatac ggcaaagagg ctcgtgatga attttctcat ttacctcttg
      541 gagaggaaga aaaaaaacgt tctatagagc cacttcgcat aatgaattta cgtccaatta
      601 gagtggtttt tagtgaaaaa gcatccgtct ctgtggatgt acctgcagat atattagttg
      661 tagaacagat gatttaataa gtaaaacgtt tggagggaat aaacgtatga aacagactga
      721 atctctttat acatataacg ataaaacaac gtttattaaa ttcactggtg atgatctttc
      781 tcgtcaagaa atgaaagggg cgaccatgcg tctcagtagt catgaattaa attttatttt
      841 attaaatagg tctccactat tcgtagaatt gtggaggtct acagatgaac aaatctgaga
      901 aaaaggcgtt actacttttt ttagtccttt ttggaatagg ctttctgatt cgattattgc
      961 cgattgaacc agtacctgcc atagaagagt tttcagtaga agaaaaagag tcattactag
     1021 aaggtgaagt gaaggatgta aaagaatctg ttgtagaaaa taaaaaagtt tcttttcccg
     1081 tgaaaaaaac aaagaaaaag gcagaaatca agtttccaat ttcgataaac aaagctactt
     1141 tagaagaact ttgtgcctta aaaggggttg gtcctaagct tgcagagaag atgattgcct
     1201 atcgggactc cgttggtgga ttcaaaacag cctctgattt gctgaaagta cccggaattg
     1261 gcaagaaaaa gtcaaaaaat attttacatg acgttatttt tgattagttt taatagcata
     1321 aatgtttcta agtcgttgaa aatatgagtg atatgaagtt acagttaggt attgaagctg
     1381 gcgatgccgc tcttaaatta tctctttttg acccacaagc caaaaaggtg ttaaagacgt
     1441 catacctgcc tacagaaaca agtcctttag atgatatata tacttttgaa accaccctac
     1501 aatcgtggtt gcaagaaaat gatttagaat caattaaatc tattgcattg acggtctcgg
     1561 tctttagagg attagtgcgt caagtttata ttccacctga agcatctagt tctattagag
     1621 attatctctc ttggactctt tctttgatga caaacataga tccagatgtt tattattttg
     1681 attatcacac attgaatggt gatgcttctc ttggttatac cgttcttttg attgcggtcc
     1741 gtcgagtctg ggtcgatgct gtgagaaagg gattccgttc taaaaaaatt gctcctacta
     1801 tttttgaatt agatgtagtt tctctttata atctgctcga gtatgatggc gctttagaaa
     1861 agggcacgga atgcttgatt aaagcggact tctcaggcgt tacgatcatg tggatggcaa
     1921 aagacgattt gaaagcgctt cgctgcgttt ctactttaga gttagtggat aagtcaaaag
     1981 aagaagctta tcttttgcta tcaaagcaag ttttagaaca actccaatta gcagatgaag
     2041 aaaatggggt ttctgtgaag agaataaggt tatgtggtga attagcgaac gatattactt
     2101 ttgtaaatat tttgagagaa cagctaaatg gatatacact tacactgctt gactcttttg
     2161 caaatatacg gttgcctgta gatgatgaaa gcaccgcttg tgtattaaat tgcgtaggag
     2221 ctattggagc ttctcttcgt tcagtaggag gaggtaatcc atgattcagt taaacttaat
     2281 tgaagctgct tctcaaactg catccctaga agctaaagtt caagtaaaat tgaacaccaa
     2341 aaataaaaca acgcttaaat cgcataagat attgattgct tttggttcgg tagctgctgt
     2401 tcttgtttta tttgctgggt ctttgatttt gtttggttta ccgaagcctt tacatggagt
     2461 gattcctgaa gcagttcttt cgactttagg agtggaagat ccaagtcgtg aagctcttct
     2521 tcaaggtaga ggaaaagcgc agatgactac ggcaggtggt ttgcttgaaa aacaacgtga
     2581 agctgaacgt gctgctctat tagagaaaca atccattagt gttgaaaaca ttgtgcaagg
     2641 tgtttctcca aaaatgttta atcaaactgt tgcaaagact cattattcca attacttgcc
     2701 gttagaacaa atagcttatc aaaatgcggc gttatcgcaa ttgcttgttt ttattaatac
     2761 ggcgactcct gataacatta gtttctctga tttgattttt gaggctccaa actattatta
     2821 tgttcgaggc gttgcagaaa atcctagttt gcaacgtagc ttcttagaac gcataaaggc
     2881 agtcagctct agttttaaaa cgcctacact gccagagaat gcgcctgtaa cggatattac
     2941 ggcttttgga gaatataatg ttgcccaacc aaatttgaat gctcataaga gtttagtcag
     3001 tgcagatggt gttgcagaag aattaaaaca ttttgctgct ctagatgtat taaaaaaaat
     3061 aaagtttagt ggtttagata aacctaagat cgaagactac ggtgttttca agaagtactc
     3121 ttttaaggct tctactaata ttgattacat ctctctgatg aactttatcg ttgaattaca
     3181 aaaatctcca gtacgactag gagttcaaaa gctagaaatg gtgccaggca aaaagaatct
     3241 tgctgcgact ctgcatctgg tgatgtatgt ggccccttaa tatatgccta ttcgagtgac
     3301 aggtggcgat tttggtggac gcctgattcc atctccttta tcgtctaaaa cacgtcctac
     3361 cgcttcaatg actcgcgaag cgctatttaa cattttattt gatgtttcag attatacagt
     3421 cctggattta tttgcaggga gtggtattgt tggaatagag gctttgagtc gaggagcaaa
     3481 atgtgtttat gcagtagaac aagcacatcg tcaggctcat gaaatttata aggcgtatca
     3541 gtctttaggc ttagaaaaat ctttacaact acttgaaaaa aatgcgcttt ctttaacgca
     3601 aggaaatctg ctagtggaag gtggctttga tttgatttat gcagatcctc cttttacaga
     3661 agagtatcct gacttgttgc catttttgaa atggctttct cctcaaggaa cagcggtctt
     3721 tgaatgccct agccgaagta taccaaaatg ggcaaaaaac gcaaaagtca agcgttatgg
     3781 agaatccacc cttttatttt ttaaatcatc tttgtaattt gactctacat ggaagaaaaa
     3841 atgaatatag ctgtatttgc cggttctttt gatccgttga ctattgggca ttatgatatc
     3901 gtgactcgag cgacttcttt ttttgatgaa gtgatagtct tagtgaattc aaatgtttct
     3961 aagcacagtc ttttttctgt ggatgccaga gtgcgtatgg ctcaagcggc tttttcttct
     4021 atggattgta ttcgtgtgga atcttttgat ggattaacgg tttcgttttt agatcgtgtt
     4081 ggcgctcgtt ttttaattcg aggcattcgt catgcacagg atcttgaata tgaacgagcc
     4141 ttgtcttgga ataatcaaaa attaaattca aaaatagaaa cgattttttt attgagcgct
     4201 cctgaacata tggctgtttc aagcagtgtg gttcgagagc ttctttctta taatcaaaat
     4261 gtagatactt atgtgccaga aaacatgaga gagattttgt tttctgagtg gagaaaatta
     4321 aatgttcggt cttaaacctt ttggttttat agcaaaacca gttagagtat taatttcaat
     4381 caacgccatc gtttttggct tggttatatt aggagccttg attggcttag attctcaagc
     4441 tttagttctt tcggttggcg ctttaattcc ttttgaattt actcaggcat ggcgcttctt
     4501 aacttataat tttgtccatg tttcatttat gcatttttta tttaatatgc tgatgctttg
     4561 gatgtttgga gatgaagtgg ctgaatttat gggccatcga aaatttgtgg gcctgtatat
     4621 attctctggt atttttgcag gagtttttag tattcctttt tatctctttg gctctctttc
     4681 gccctatgct atgattattg gtgcttctgg tgctctaatg ggggtctttg tagcttatta
     4741 taagtttttc cctgaacgga cattgttgtt gttttttgta atccctttga aaatgaaata
     4801 tgcaatatgg gttttaattg cacttgatat tatgatgtcg caaacaaatg attctattgc
     4861 ccatttcaca catttaggtg gagtgttagc gggctttatt tttatgaatt tcttttatcg
     4921 tattccattt tgggaaaaat ggtttcaacc taaaagaaaa aaacagcact ctcaacataa
     4981 tagttcagaa aaggtgttag aaggcgaagt aggttatctt gatcaagata aacaaatgga
     5041 tgcgattctt tctaaggtca ataaagaagg tgtaaattcg ctgactgaat cagaacgtca
     5101 atatttgtta aaagctggag aacgtctgcg ccgtcgacgt ggtctataaa gaaaatattt
     5161 ttaatggcaa ataaggataa tagatagatg aaaaagatat taggtatggg cgccgcactt
     5221 gtagatgttg tctatactgt agaggatgct tgggttaagg cttctggaag tgaaaagggg
     5281 ggcatgactt taattgatca aaagaaaagt caagcatggt tagcagaagt aaaaaatcca
     5341 gtattagttc ctggtggttc tgcctgcaat acgattgttg gactttctaa gcttggagcc
     5401 cgcgctgctt ttattgcaaa gattgggcat gatgagttag gaaaacgttt tgaaactcat
     5461 ttgagtgatt ctcatgtcga atccaaactt cttttttcag ataagcccac aggatctgtt
     5521 ttagccgcag tgactccaga tgcgcagcgc acgatgttta cttatttagg tgcatcggat
     5581 gatttagatg tagatgatat tcaagattct ttatttcaag atgtatctct attttatata
     5641 gaggggtatc gtgcatataa tggcccttgt tttaagaagg ccttacaact ggctaaaaag
     5701 aatggcgtga ctactgtggt ggatttcggc gcatttagtg ttgtgaatga ttgtcgcgct
     5761 ctttttgata acttgtttca agaaaagtta gtagatgtta tcattgcgaa cgaagatgaa
     5821 gctcttgcct atacgggtaa aacggaagaa gaggcgttag ctgttttagc ttcaaaatct
     5881 tctgttgctg tagtgaaatt aggaaagcgc ggcgcattga tctctaaaga cggaaaagtc
     5941 acttctgtag atgcagagca agtgaatgcg attgatacaa caggagcagg ggacctttgg
     6001 gcggctggtt ttctatttgc ctatttagaa ggagagtctc tttctgtttg cggtcgctta
     6061 gcaagtcttg tggcttcaga agtggtacaa gtaatgggtc cacaaattcc tgaagaaggc
     6121 tggtttagaa ttaagcattt ttttagataa gcataaaatt aaaagctctc ttttgaaaga
     6181 gggctttttt aataatgaat attttaagaa tcaaatagaa attgttttag aagtgccttt
     6241 taataaatcc caacaggaat atcgatttga gattgaagtt ggttcgctac atcttccata
     6301 aatgatttcg agactggtaa aacatgggga cgtgccgtat ttcgaactac gctgtataat
     6361 tgcacttcaa gaaggttttt actgtgatga tgatatatct ttttaactct ttcaatgtac
     6421 ttacgaactt cttcgggaga agggcttatg ccatctactt cacaccacat cgtttgaatt
     6481 cgagtaggaa aatcttttac ggtaagctca atatttcttt gaatcacttc taaagggtat
     6541 gggctgatat ttactttttg aaaccactct tctgtgcccg catctaattt agcccagatt
     6601 tctccattag tttgagttaa atagcgaagc ccctcgcgca cgtactcttg atcgagtctt
     6661 gtggaattcg tgatgagagt gagtttcaaa ttgtatttag aatatttatg ctggatttca
     6721 agaagacgtt cacacacggc agggaattct ttaatcaaag tcgattcgcc atctccagaa
     6781 agacagatgt cttttatttg acgctttgat tcttcaataa gcttaaagtt ggaaaaagaa
     6841 gtaagcccat tcttttcata ttcttggata atgagttcta tttctttggt catcgcctca
     6901 atatctaggc gcttattaga tcctggaata gatcgatcca cctgacaata aacacagtca
     6961 aaggaacaac gcttatcggg atttaaattc actccaatag aaagcccttt ggctctcctg
     7021 ctaatgacag gataaaccca ggtgtttttc tcccaaagtc tagagtggtc tgaccaagcc
     7081 tcttttaaaa aagaatcact catctttaat atacccacat aaatcacaag ttaatttgta
     7141 gctgttgtcg ggctctactt ttaatacccc gcgacaattt tcaacagggc aggggagttc
     7201 cccaatttct aattctctat aggtttcttt ctctttcatt cgtctaaata taatttttgt
     7261 tcaaggcact tatcgtaaac aaaaggatta tgattccctt gaacattata aatacgttta
     7321 tctctaaggc attcttttga attgacgggg tacattcgat cccatgcttc aaataatttg
     7381 cggtcttgat tagaaagcga aacgccgtat gttttttcca tgtaaaaaga aactcgtgca
     7441 ataatgcctc gcgcttcttt tcgaggttgg acttttttga gttcgaaatc cacaatcgtc
     7501 gaacatttcc catacatagg ttcaggctcg tttgtccatt gagaaaacat gaagttgctt
     7561 cgatccccgt tgacttctcc aatcgcaggg taaaggttat ggagatcgcc ttccattaaa
     7621 tcgaattgct cattattgtt agaacagttt tttcgcccgc cttctttcca gcaaggaaga
     7681 aaatgcccca tattatgagc tgtgacaata tgttcccatt caatgcggtg ggcacgtcta
     7741 aagcttttac gttttggatt ttctcgaggg gcgtaatcac aagaattaaa atcaatactc
     7801 tttttatttt tataactaca cccacaatag agcgtctctt gaagctcgcc ataataaatg
     7861 cgatgcattt gccgtgaggc accgttgtaa tcataatgcc taggttcttc ctcagcgaat
     7921 aaagtacacg ccagaagaat caatagggta aagactaacc gtttcattta taaataagat
     7981 gtattaaaag aatatttaaa acagttttac gctaaaatgg aatcaattaa ttgaagctct
     8041 tctgaagtaa aggaaagatt ttgcagagcg cctaaagcat cttgaatttg ctctgttcga
     8101 ctggctccta ttaaaacaga agtgacttct ggaagcctta aattaaaggc aagggctagt
     8161 tgagacaatt tttgaccacg tttttttgca agctcattta attgttttac tttagataag
     8221 cttgattcta cttggtcttt tgttaaaaat ggactcttag ctgctctaga atcacttgga
     8281 ataccattta agtaacgatc ggtcagtagg ccttgagcca gtggcgagaa ggtgatacaa
     8341 ccaatcccat tcttatttag aaccgattgt agattccctt ctaaggaacg attaaacata
     8401 tgatattgtg gctggtgaat taagcaaggt gtgcccattg attttagaat atcataggca
     8461 gcttgagtag aaggcccgtc atagcttgaa ataccaacat aaagagcacg accactttta
     8521 acgatatgat ctaaggctcc cattgtttct tcaagaggtg tctctggatc aggacgatga
     8581 tgataaaaga tatcgacata atcaagaccc atgcgtttta agctttgatc aagacttgca
     8641 attaaatact ttctgcttcc aaaatctcca taagggcctg gccacatacc ccaaccagct
     8701 tttgtagaaa tagtcatttc atcacggtaa gagagaaaat ctttttttag aatctgacca
     8761 aatgtttctt cggcacttcc tgctggaggg ccataattat tcgcgagatc aaaatgacag
     8821 accccatggt caaaagcgta gcgtactttt tctctagaat tttcaaaaag atctacgcca
     8881 ccaaaattat gccaaaagcc aagtgaaacg gctgggagtt ttaatccact tttaccacag
     8941 cgacggtaaa tcatagaatc atatcgatta gatgcaggag aatatgtcat aataaagcct
     9001 cactattttt taggaatata taaataaaat aaaacgtgtg tttgtgataa atattatggt
     9061 ggccttaata agtaccctat ttagttttgt aaaacaatat ataaaggttt ctttaattag
     9121 ttattattga gggtatgaaa aaaagtggaa tggatttttt gaatgattct ttattgtatg
     9181 gtatgaaacc tggtcttcag aatatggaaa ccatgtgcag gtattttaat catccagaaa
     9241 agtcttttca agtgattcat gttgtaggga ctaatggcaa aggctcttcg gcgtattatt
     9301 tagcccaaat actcaaggcc catgaaaaaa aggctggcct attttctagc cctcatttag
     9361 tgagcttaca agagcgtatt cgtgtgaatc aagaagccat ttctaaagcc gatttagagt
     9421 ctactctttt agaagtacaa aaagcagcca tagaaaaaaa aattagccct actttttttg
     9481 aaatcatgac gatggtttgc ttgttgtatt ttagagaaca gcaaattgaa gtggccgttt
     9541 tagaagcagg cctcggtggt cgtttagata gtacggctat cgctaagggc aagtactgcg
     9601 ttttaacaag tatcggtcta gatcatacgg aaatattagg ggattcttta tcttctattc
     9661 taaatgaaaa gttagggatt ttatctgcgg gttcgttact cattcgcttt tccctaggtt
     9721 ctaatttaga cacccaagca agccaattag taaaagaaaa atacgcgagt gaaatggtcg
     9781 ttgaaagaga tttatcgcta gtccttttaa atggtggcac tcattttaaa gaaaacgcca
     9841 cgttatcctt aaaggctgca gaactcgttt taggcaaaga atggaagaac tctgttgcat
     9901 tagaggcact ttcgcaaaat gcttgggctg gcagaatgca tctactaaaa aataaaaatg
     9961 agaagctaga gtttatctta gatggcgctc ataatttaca cgccatggaa cgattagtag
    10021 aaaccttaaa tgcggatttt catggcatga cgttcccttg tgtatttggt gcagtcaaag
    10081 acaaagacgc gagcgatatg attcgtttac tagctccgtt tgtctctaaa tggtatttca
    10141 caagaacgcc gtatatgcgc tttagagaaa cagaagaact ggctgaaatc cttttacaat
    10201 atggtaaaac accagagaga caatttatgc tttctaaaga agatttgctc gagtgtcaaa
    10261 aagaagcaaa tggtgcccct attttagtaa caggttctct ttatttaatt ggcggcgttg
    10321 tggatctatt aaagaatgat tttgaatcgc tttctttttt cagagggctt gaagccacca
    10381 caaatgaaca tcgatagatt tagaagaaag ttttaagctt ttgaagcact tcagaaacat
    10441 ctgtaaaggc acatactggt aaactgaaca cttgtttgca gactaagtct gtggaaggat
    10501 tctctgggtt acttaggcct aaaaaacaag gctgtttacg caatgctttt ggataatgaa
    10561 tacaataggg aataccacat tctttcaaaa tagattccat ctgttttcga tttgaagacc
    10621 gaagcgtgta ttgagcgtaa gtagaagaat ttccagatag aatttgcgga atggacnnnn
    10681 nnnnnnaaaa agaattgtat tgaagagcat tttctcttcg aatagataat tcattgtcta
    10741 aatattttaa cttgattcga agcgttgccg cttggataga atctaaacga ctatttctac
    10801 caatcagctc atgataataa cggatagaag agccatgatt ggcaagagaa cgaatacgat
    10861 cggctagttt ttcggagtaa gtgaataagg caccaccatc cccaaaacaa ccaagaggct
    10921 ttgtggggta aaaactggtg atcgccatgt ttccaaagga acccgctttt ttcccatttt
    10981 gagaggcacc aaaactttgt gctgcatctt ctaaaatcca gagatggtat ttttgactga
    11041 ttttagaaag agcttcataa ggagcacatt gcccaaatag atcaacggct atgattcctt
    11101 tagtctttga agtaatgaga ctctccacag aagaagggtc aattaaaaaa gtattaggat
    11161 taatatcaca aaaaacaggc acaccaccta aactacgaac agcttctgca ggagcaataa
    11221 aagaaaaatc aggaacaatc acttcatcac ctggctgtag accaagagct tctaaagcta
    11281 agaagagagc gtcagtaccg cttgcgcaag taattgcata aggaacttga acatagtgag
    11341 caagttcttc ttctaatgca gaaacatcag ggcctccaat ataagctcca gaataaagaa
    11401 cgcgatctat ggcgctttta agttcagttt cgtgtgcttt gtattgagcc gaaagattaa
    11461 caaatggaat catggccaca aaattataaa aacatctaag ccaaatcatg ttttttataa
    11521 aaagacatcc caaaaaaatc ataatccaaa aaagaaccgt gacacattga agaaattact
    11581 gcgtatggat ctcattaaag gagtagcccc ctccgccttc atgattgaaa agcatttcct
    11641 cgcgtcctca tctttggtgc tgatattcat taaaaagatg gttttttatt aggtaaagtt
    11701 ggtttatatt gcgacgcctt tctgctaaaa acttatattg attttatgca gcgtattcat
    11761 aaaatactcg gtttgcgttt agctggggaa cggccattac attataggta tacatttata
    11821 gtaaatccat ggtaaaatat tttctattac ttatagcctt tttctttttt atttcttgtt
    11881 cttataagaa tgtggaaaga cttaattttg ttgggattat ggaaggctct cctcaatcct
    11941 tagataaata ttctctagtc tattatattg atgagcaaca ggtaaatgat gtttacaaaa
    12001 ctaggcgact ttcttttaat agtataaatg aagagtcaaa tataggtaga ctagaattat
    12061 atggtgctgg cggctactgt aatgtctata tgaagaatgg gcagtttgct aattgtcttc
    12121 atccattaag aggtcagttt tttgtaaagg tgttcttaag gcataatgag acaggagata
    12181 gcgtacttgt tgggaaacat tttatagata tggatgggta tttattaaca tcagttagaa
    12241 cagaggtgta tttaggccct aacaaagaag aatatgaacc tagaaattgt tataattttg
    12301 at
//
LOCUS       DAOOBB010000043        12219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_6659_length_12219_cov_5.027458, whole genome shotgun sequence.
ACCESSION   DAOOBB010000043 DAOOBB010000000
VERSION     DAOOBB010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 12219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12219
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6659_length_12219_cov_5.027458"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /gene="pheS"
                     /locus_tag="PLT31_03405"
     CDS             <1..963
                     /gene="pheS"
                     /locus_tag="PLT31_03405"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPW94215.1"
                     /translation="LTSKEEVDAIRVKYLGKKGLVTDLLKQMGALPAEERPAYGKKVN
                     ELKEFVSESIENALLVAAEAALQKKLASGFVDISLPENGIPAGSTHPLYDVREEIIDF
                     FAHMGFEVDFGRDIETDWYNFEALNTPPDHPSRDMQDTFYLADKVMLRTHTSGTQIHY
                     METHKPPFRMIAPGHVFRVDSDATHAPMFQQCEGLVVDENITFAQLKGVLQVFMNKLF
                     GSGVKTRFRPSFFPFTEPSAEMDVSCVFCKGEGCRRCKGTGWMEIGGCGSVDPNVFIN
                     CGIDPERYTGFAFGFGLDRIAMLRHAIPEIGLLTGNDERFLSQF"
     gene            complement(1050..1862)
                     /locus_tag="PLT31_03410"
     CDS             complement(1050..1862)
                     /locus_tag="PLT31_03410"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPW94216.1"
                     /translation="MKKNSLFIFILSFLFLFSTSLWAQEEEVSSEETSEDSTSYESSN
                     ASEFEDDEDYAEAYQRYQEQKTSSDLIASERIRNWSDAVTIGLKAGLGSPTFLGDQGN
                     GWSFGLAVNLGAIMKIRITDYFALTPELTASYRNLSREINENDAYTVETSLSNIVIEI
                     PILLRFALDNTEGLYAFTGPEIGLVVYNNSSEEVTFETSSSPTTEELDPIESSSVEIG
                     FVLGVGYQVNRWLGLDLRAFYSFTNYSNETLSTDDYKNINTLFFQFSASYFF"
     gene            2007..4718
                     /gene="leuS"
                     /locus_tag="PLT31_03415"
     CDS             2007..4718
                     /gene="leuS"
                     /locus_tag="PLT31_03415"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HPW94217.1"
                     /translation="MAKYNPSAIEPKWQHFWDEKHTFKTGEDPSKPKYYCLDMFPYPS
                     GAGLHVGHPEGYTATDIICRYKRAKGFNVLHPMGWDAFGLPAEQYAIQTGTHPNVTTQ
                     KNCDNFRGQIKRLGLSYDWDREINTTDPGYYKWTQWIFSQLYNTWFDNSIQKGRPISE
                     LPIPAEIEKQGKVAIREYKDSKRLAYYADAQVWWCKHCRIVCANEEVLNDGSHEKCGT
                     KEVERRQLKQWLMRIPHYGERLLEGLDQLDWPEGIKDMQRNWIGKSRGAEVDFTIANP
                     DGSAGTRRLKVYTTRPDTLFGATYMVVAPEHALVSDLLDSGEMGVSAEIKKAVSAYVK
                     AAALKSDMDRTELAKEKTGVFTGLYAVNPLTHNLIPIWVADYVLTGYGTGAIMAVPAH
                     DTRDFEFAQKFNLPVICIMEPDSACPPETAALVLEGKACWTADGKYIKSSNDTLSLNG
                     LSKKEGIDKVIAYLQENKLGQAAVNYKLRDWLFSRQRYWGEPFPVIHWEDGEITLLNE
                     NELPLELPEMADFKPGEGGESPLANAGEWLNVVDANGRKGRRETNTMPQWAGSCWYYL
                     RYIDPKNATSFLGKEKENYWMPVDLYVGGAEHAVLHLLYSRFWHKVLFDLGHVSTPEP
                     FHKLFNQGMILAYAYEDAAGSKVPSDEVEERDGLFYKKGTSIQLKQIIAKMSKSLKNV
                     VNPDDVVNQYGADSLRLYEMFMGPLDAVKPWQSQGLEGMHRFLSRVWRAIIGDDDDVI
                     AVYENEGAPESLIKVMHQSIIKVTEDIEKMAFNTAISQLMIFNNELTKTTKRFREPCE
                     TFVKLLHPFAPHLAEELWSLLGHENSLTNESWPVADVSKTVENTVEVVFQVNGKVRAR
                     EKVSVSLDKETLENMAINHERVKEYTTGKTIVKAIVVPGKLVNFVVK"
     gene            4883..5128
                     /locus_tag="PLT31_03420"
     CDS             4883..5128
                     /locus_tag="PLT31_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94218.1"
                     /translation="MQKKVTRSIYGSYKLLMTIENKSTYIIFRVRSSTSIEEMKSSAS
                     TISSSHKLVVRNNKVELQKKTNTGKMIYYNLHGSRVK"
     gene            complement(5206..5958)
                     /locus_tag="PLT31_03425"
     CDS             complement(5206..5958)
                     /locus_tag="PLT31_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94219.1"
                     /translation="MKKYIYIGILASILLSACLFDGEDNSKNNTTPAKGTLHSAFVGD
                     WLFVYKTSTEQELWQWAFNADGSMNSCRWETSADGSLSSSHLCVEGSWSDVGDVATLW
                     PELYPTLEQYTRFPVSKGHAILTPIDTSKSNLRRWSQGWLLYARNDTLTYLGTIIILD
                     GGPQLYGDPWTYQDKTFIFYEDETCSMLNNDSSNCSWGIRETDVLKISDVLEISWDAG
                     NGEKDYLDGHFAIHEGKILLLDLEDSADFFRQ"
     gene            6340..7158
                     /locus_tag="PLT31_03430"
     CDS             6340..7158
                     /locus_tag="PLT31_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94220.1"
                     /translation="MVEKQTKLQKYNQRIIAIVGTLFLVILLIALIAILKELVDELFP
                     DETQPYASVFSEEKQDASSSENLVTQQISYGKPILVDTTAMVFVIPVHNEIVNKTKSK
                     SLFARFAFYETVVEEVIFEYSKIYSRDFNNLILWDYNSNSKQVLLKDRIAAGGLTRVK
                     VNGKRYLVFVIADTDSNKDGLINNLDNISLLIYSLDTQEKRKIAYDNATLISYHYLKG
                     RNEIFMEFKKNQQKSEEKVIQVEPSFFMLYDYEKETMTPVVDPETDQSLKKMYE"
     gene            7186..7932
                     /locus_tag="PLT31_03435"
     CDS             7186..7932
                     /locus_tag="PLT31_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94221.1"
                     /translation="MFNKIIFGAFALLLLACNNGPKIMPPLEIQAISNDVVIMADPSD
                     SLTLSVAANQATIMANDYFDLQQKIGVDSILNKSIVISKKEGTLCSKKILESTNDNVK
                     IIVYGCDDEVGVVSILLRELEGKSSDSLIIYMDENNNVLHGFISPNHSLSDLYYGGGY
                     EFQYNKETKEMELINLDFGKIRTIPDRSLMHVRSSSKSNTPTVTDLFQFLTNKYFLPN
                     VYVMTKDQSFWQMFFSYKENKNYIIKKQMD"
     gene            complement(7985..8635)
                     /locus_tag="PLT31_03440"
     CDS             complement(7985..8635)
                     /locus_tag="PLT31_03440"
                     /inference="COORDINATES: protein motif:HMM:NF024845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HPW94222.1"
                     /translation="MARCKNCGAPLLIGSSICGYCKSSVDIDLSGRHYYTSNLPKKKR
                     PCPNCKIEMDVLNITRDEKPFYLERCPKCLGLFFDIGELDLFLKEYSNGVRSMNHSHL
                     SALRSIAGKAMENATFYKPCPVCGKLMNQQNYGQVSGVVIDRCRDHGVFLEAGELQKL
                     AMWIKAGGHLQEVPDNKVYKREKEIKDEHDYSFLKNEAKEGGFFDSLLDVIFEFMK"
     gene            complement(8637..9707)
                     /locus_tag="PLT31_03445"
     CDS             complement(8637..9707)
                     /locus_tag="PLT31_03445"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPW94223.1"
                     /translation="MSLLDKIIGEFIDIIEWLEDGSDTLLWRFPRYQNEIKNNAKLTV
                     REGQVAIFVNEGKVADVFQPGMYTLNTKNLPILSTLKGWKYGFDSPFKAEVYFISTRR
                     FTDQKWGTRNPIILKDPEIGPVRLRAYGTYILQSTDPLLLLKEVLGSKKEFSMAIISE
                     QIRNLIVAAFADAIGESKIGVFQLAENYDTLSVGISKKIATEIKGYGLSCSQLLIENI
                     SLPEEAERSIDKKSSMGVVGEMDYTRYQMATAAETAATHSGEASAGIGLGMGIGMAQQ
                     MTQSVSNPPPIPGSSLWWVAINGKKNGPHSLEALSSLFQKNNLTAETLVWKQGMDNWS
                     AAKSRPELSSLLNQTPPPIPEK"
     gene            complement(10004..10705)
                     /locus_tag="PLT31_03450"
     CDS             complement(10004..10705)
                     /locus_tag="PLT31_03450"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPW94224.1"
                     /translation="MMNTDALPPENSVNDETEEIKTHRPWQRKKEIRPQEILAAALNV
                     FVEQGFSAAKVAEVARRAGVQPGTIYVYFENKEALFKAVIHAAMSPVLSYSNELLDNY
                     TGTPEKLLYELTHQWWNLMESESCAGIPKLITAEANNFPELTQFYIDQVLVHAKRIVE
                     RVLQYGIDRNQFFIDDMDITSRMIMMSLHEMTVYAHSFDHFDKMKIDVPKYLDTLAAF
                     LYKGLSCQKRQDPET"
     gene            complement(10795..12075)
                     /locus_tag="PLT31_03455"
     CDS             complement(10795..12075)
                     /locus_tag="PLT31_03455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPW94225.1"
                     /translation="MSVESLYNVVDRFFVAEEVGYLGITGITLCFPISLFIMAMSILI
                     GVGGNTLFSLKLGQKKYRQAAMVLNHSLVLLTIMSVIVFALGQIFLEPLLKVFGASEV
                     TLPYAKTYMSIILCGVFFQTINPGMNNFIRSMGHPKTAMFRMLIGAFCNVFFDWLFII
                     KFHWGIAGAAWATVLSQFISFLFIMWFFFKKNTPIKIQSRFMHLQFPFVRRLLIFGLP
                     MSMMQICNSLMNVILNKSLATYGNQSVYGGDLAISAFGVINSIAMMIIMPLLGFVQGI
                     QPIVGFNYGAKNYSRVKSALKYAFVLAVSFLSIAWILIQVFAPNLVQPFASNNRELQE
                     LATHSMKIFLLGLPAIGVGMICGNYFQATGKPGRSIILNMSRQILILIPLLLIIPNFF
                     GLSGVFMAAPISDTLAALLGVFLLSKEWKKMPNQ"
BASE COUNT         4024 a         2072 c         2367 g         3756 t
ORIGIN      
        1 ttaacttcta aagaagaagt ggatgctatt cgtgtcaagt acctcggtaa aaaaggctta
       61 gttaccgatc tactaaaaca aatgggggca cttcctgcag aagaacgccc tgcttacggt
      121 aaaaaagtta acgagctaaa agaatttgta tctgaatcaa tagaaaacgc tcttttggtt
      181 gctgctgaag ctgctttgca aaaaaaacta gcgagtggat ttgttgatat ttcgcttcca
      241 gaaaatggaa ttcctgctgg tagtactcat cctctgtatg atgttagaga agagatcata
      301 gacttttttg ctcatatggg atttgaagtt gattttggtc gtgatattga aaccgattgg
      361 tacaactttg aagctttgaa tacgcctcca gatcatccaa gtcgcgatat gcaagacacg
      421 ttttatcttg cagacaaggt gatgcttcga acccatacgt ctggaactca aattcattat
      481 atggaaacgc acaaacctcc gtttcgtatg attgctccag gtcatgtttt tagagtagat
      541 agcgatgcta cacatgcacc aatgttccaa caatgtgaag gtcttgtcgt tgatgaaaac
      601 attacttttg ctcaattgaa aggcgttctt caagtattta tgaataagct ttttggctct
      661 ggtgtaaaga caagattccg ccccagtttt ttcccgttta ccgaaccatc tgctgaaatg
      721 gatgtgagtt gcgttttctg taaaggagaa ggttgtcgac gctgtaaagg aactggctgg
      781 atggaaatcg gtggatgcgg ttcagtagat cctaatgttt tcattaattg tggaattgac
      841 cctgaacgct atactggatt tgcttttggc tttggcttag atcgaattgc catgcttcgt
      901 catgcaatac cagaaattgg ccttttaaca gggaatgatg aacgtttcct ttctcaattt
      961 tagttcgcta ttttatatga tttaaaaaaa agaactccgt tagtttagct agcggagttc
     1021 ttttataaaa gcaatatgat taaactatct tagaaaaaat aggatgcgct aaactggaag
     1081 aagagcgtgt tgatgttttt ataatcatcg gttgatagag tttcattaga gtaatttgtg
     1141 aagctataaa aggctctcaa atctaaacca agccagcgat tgacctgata accaacgccc
     1201 aagacaaatc cgatttctac tgaactagat tcaattggat ctagttcttc tgttgtaggt
     1261 gaggagcttg tttcaaaagt gacttcctca gaactattat tataaacgac taaaccgatt
     1321 tctggtccag tgaaggcata gagcccttca gtattatcta aggcaaatct tagaagaatc
     1381 ggaatttcga ttacgatatt tgaaagcgat gtttctaccg tataggcatc attctcattg
     1441 atttctctag ataaatttcg atagcttgcg gtgagctcag gtgttaacgc aaaataatcc
     1501 gtaatgcgaa ttttcatgat tgcgccaaga ttgacggcaa gtccaaaaga ccaaccatta
     1561 ccttgatcgc ccaaaaatgt aggagatcca agccctgctt ttaagccgat agtaacagca
     1621 tcagaccaat tgcgtatgcg ttcacttgca attaagtcgg aagaagtctt ctgctcttga
     1681 tagcgttggt aggcttcagc gtaatcctca tcatcttcaa attcacttgc attagaggat
     1741 tcataagaag tactatcttc tgaagtctct tctgaagaga cttcttcttc ttgagcccaa
     1801 agagaagtgg aaaaaagaaa gaggaaagaa agaataaaga taaataagct gttctttttc
     1861 atatgaaaaa aagtagtaaa aaaataatgt ctttatagaa tcaagaatgt taaatgcgtg
     1921 taacattcaa cccattaaag ttttttttgt tctttttttc tagatttgac cccaaaaaag
     1981 cattaaatcc gatttcagga gtttttatgg ctaagtataa cccttcggca atagaaccta
     2041 aatggcaaca tttttgggat gaaaaacata cttttaagac aggggaagat ccatcaaaac
     2101 ctaaatatta ctgcttagat atgtttcctt acccaagtgg agcgggtctt catgtcggcc
     2161 atccagaagg ttatacggca acagatatta tttgccgtta taaaagagcc aaaggattta
     2221 acgttttaca cccaatgggt tgggatgcat ttggccttcc agcagaacag tatgcaattc
     2281 aaactggaac gcatccaaat gtaacgacac aaaaaaattg tgataacttt agaggccaga
     2341 ttaaacgatt gggtttatct tatgattggg atcgtgaaat caatacaaca gatcctggtt
     2401 attataaatg gactcaatgg atttttagtc agctctataa tacttggttc gataattcta
     2461 ttcagaaggg tcgccccatt tcagaactcc caattcctgc agaaatagaa aaacagggaa
     2521 aagtcgccat acgtgagtat aaagattcaa aacgactcgc ttattatgcg gatgcgcaag
     2581 tgtggtggtg taagcattgc cgtattgtat gtgctaacga agaagtatta aatgatggat
     2641 ctcatgaaaa gtgcggcaca aaagaagtgg aacgccgcca attaaaacaa tggctgatgc
     2701 gaattcctca ttatggggaa cgtttattag aaggtcttga ccaattggat tggccagaag
     2761 gcatcaaaga catgcaacgc aattggattg gcaaaagtcg tggtgctgaa gttgatttta
     2821 ctattgctaa tccagatggt tctgctggaa cacgtcgctt aaaagtctat accactcgtc
     2881 cagacacttt gtttggtgca acatatatgg tggtggctcc tgaacatgca ttagtttctg
     2941 atttattaga ttcaggcgaa atgggtgtat ctgctgagat caagaaagct gtttctgctt
     3001 atgtaaaagc ggctgcttta aaaagcgata tggatcgaac ggagcttgcg aaagaaaaaa
     3061 caggcgtttt tactggctta tatgcggtca atcctctcac gcataatctc attcctattt
     3121 gggttgcaga ttatgtctta acgggttatg gtacaggagc tattatggca gtccctgctc
     3181 atgatacgcg tgattttgaa tttgctcaaa agttcaatct accagttatt tgtatcatgg
     3241 aaccagatag tgcatgccct ccagagacgg ccgcccttgt tttagaaggc aaagcttgtt
     3301 ggactgcaga tggaaaatac attaagagtt caaatgatac tctttcctta aatggattgt
     3361 ctaaaaaaga aggcattgat aaagtgattg cctatcttca agagaataag ttggggcaag
     3421 cagctgttaa ttacaaactt cgtgattggc tctttagtcg tcaacgttat tggggtgaac
     3481 cgttccctgt aattcattgg gaagatggcg aaattacttt gttgaatgaa aacgaattgc
     3541 ctttggagct ccctgaaatg gctgacttta aacctggaga aggaggcgag tctcctttgg
     3601 ccaatgcagg cgaatggttg aatgttgttg atgccaatgg gcgaaaaggt cgtagagaaa
     3661 caaataccat gcctcaatgg gctggtagct gttggtatta cttgcgctat attgatccta
     3721 aaaatgcaac ttccttttta ggtaaagaaa aagaaaatta ttggatgcct gtcgatttat
     3781 atgtcggtgg tgcagaacat gccgttttac atttgcttta tagccgtttt tggcacaagg
     3841 ttctctttga tttagggcac gtgagtacgc cagaaccctt tcataagttg ttcaatcaag
     3901 gcatgatcct tgcttatgct tatgaagatg ctgctggctc aaaagtacct tccgatgaag
     3961 tggaagaacg tgatggcttg ttttataaaa aagggacttc cattcaatta aaacagatta
     4021 tcgcgaagat gagtaagtcg cttaaaaacg tcgtgaatcc tgatgatgtt gtgaatcaat
     4081 acggagcgga ttctcttcgt ttatatgaga tgttcatggg gccattagat gctgtaaaac
     4141 catggcaatc gcaaggctta gaagggatgc atcgcttttt atctcgtgtt tggcgtgcga
     4201 ttattggtga tgatgacgat gttattgcag tgtatgaaaa tgaaggtgct cctgaaagtc
     4261 tcattaaggt aatgcatcaa agcattatta aggtgactga agatattgaa aagatggctt
     4321 ttaatacagc gattagtcag ttgatgatat ttaataatga gttaacaaaa acgacgaagc
     4381 gtttccgtga accatgtgaa acttttgtga agttacttca tcctttcgct cctcatcttg
     4441 cagaagaact ttggagcctt ttagggcatg aaaattcttt aacaaacgaa tcatggcctg
     4501 tcgcagacgt ttctaagact gtggaaaata cggtggaagt cgttttccag gtcaatggaa
     4561 aggtgcgcgc aagagaaaaa gtcagtgtct cattagataa agaaactctt gagaatatgg
     4621 cgataaatca tgaacgagta aaagaatata ctacagggaa gacaattgtg aaagcaattg
     4681 tagttcctgg taaattagtg aattttgtgg tgaagtaata ttcgcttgat cacttgaaaa
     4741 cagtttctta aaattagcaa attaatcctt tagtattaac agatgaaaac ttcgaaagag
     4801 aatttctttt ttgaagtttt tttataaaac gacgcaaaaa acaatgaaaa aaagaatgat
     4861 gaaattctct taatttttta tattgcaaaa aaaagtcact aggtccattt atggatctta
     4921 taagttatta atgactatag aaaataaatc cacgtacatt atttttagag ttcgctcttc
     4981 aacatcaata gaagaaatga agtcatcggc atctactatt tcttcttcac acaaacttgt
     5041 tgttagaaat aataaagtag aattacagaa aaagacaaat actggaaaaa tgatatacta
     5101 taacttacat ggctctagag tcaaataaat cataaaaaag aatcatcccg tttatgatta
     5161 acataagcgg gatttttcta tagaaagtct ttgttttaga ttattttact gacgaaaaaa
     5221 atcggctgaa tcttctaagt ctaaaagaag aattttccct tcgtggatag cgaaatggcc
     5281 atctaagtaa tctttttcgc catttccagc atcccaagag atctctagta catcggagat
     5341 ctttagtaca tcggtttctc ttattcccca agaacaatta gaagaatcat tgttaagcat
     5401 agagcaagtc tcgtcttcat aaaaaataaa tgttttatct tgatatgtcc aaggatcgcc
     5461 ataaagttga ggccctccat ctagaattat gatcgtacct aagtaagtta aggtgtcgtt
     5521 tctggcgtaa agtaaccatc cttgactcca tcttcttaaa ttggattttg aagtatctat
     5581 cggggtgaga atcgcatggc ccttagaaac aggaaaccgt gtgtattgtt ctaatgttgg
     5641 ataaagctct ggccacaggg tcgctacatc accgacatcg ctccaagaac cttcaacaca
     5701 taaatgtgaa ctagatagcg atccatcggc agacgtttcc cagcggcagg aattcataga
     5761 gccatccgca ttaaaagccc attgccataa ttcttgttct gtactcgttt tatatacgaa
     5821 taaccaatct cctacaaaag cagagtggag tgtgccttta gcaggagttg tattattctt
     5881 gctattatct tcaccatcga ataggcatgc cgataataga atgctcgcta aaataccgat
     5941 ataaatatat tttttcatat gctcctctaa aaaataattg gagaagaatt tattaattta
     6001 tcttaatatt tatataatat aataataaaa ggaaccgatg ctttctagtg tatcaagaaa
     6061 ggaacatatt gaggagaaac atacaaatga aattaacaaa tgaaggagtc ggccaagtta
     6121 tttcaaaatt aggaataatt tattttggtg taggatgtgt ttctagcttt ataagatgag
     6181 ttttatttga atgagacatt ttgcccaaag atgttcagcg gaacgattgc tattattctt
     6241 tcatttggtg ttctgaagca taagattata tataaaaaat aaacatgtgt ttataaaaca
     6301 cgatataaaa agcaataaat catttgaaat aaggagaaaa tggtggaaaa gcaaactaaa
     6361 ctacagaagt ataatcaaag gataatcgcc attgtaggga cgttgttctt ggtaattctt
     6421 cttatcgcgc tgattgcgat tttgaaagag cttgtagacg agcttttccc agatgaaaca
     6481 cagccttatg ctagcgtatt ttccgaagaa aaacaagatg catcatcttc agaaaattta
     6541 gtgactcagc aaataagtta tggcaaaccg atattagtgg atacgactgc aatggttttt
     6601 gtcattcctg ttcataatga aatagtgaat aaaacaaaaa gtaaatcact ctttgctcga
     6661 tttgcttttt atgaaacggt tgttgaagaa gtgatttttg aatattcaaa aatttattca
     6721 agagatttta ataacttaat cctttgggat tataattcaa attcaaaaca agttttacta
     6781 aaagatagaa tagctgcagg tggtcttaca agagttaaag tcaatggaaa aaggtatctt
     6841 gtttttgtga ttgctgatac tgattctaat aaagatggtt tgattaataa tttggataat
     6901 atttctttgc tgatttattc tttagacacg caagagaaac gaaaaatcgc atacgataat
     6961 gcaacactta tatcttatca ctatttaaaa gggcgtaatg aaatatttat ggaattcaaa
     7021 aagaatcaac aaaaaagtga agaaaaagta attcaagtag aaccgtcatt ttttatgctt
     7081 tatgattatg aaaaagaaac aatgactcct gttgttgatc ctgaaacaga tcaaagcttg
     7141 aaaaaaatgt atgaataatt atcttagact attggagtat atatcatgtt taataaaata
     7201 atatttgggg cttttgctct tttacttttg gcttgtaata atggacctaa aatcatgcct
     7261 ccattggaaa tacaggctat tagtaatgat gtagtgatta tggctgatcc tagtgattct
     7321 ttgactttaa gtgtagctgc taatcaagcg acgatcatgg caaatgatta ttttgattta
     7381 caacaaaaaa ttggcgttga ttctatctta aataaaagca ttgtgatttc aaaaaaagaa
     7441 ggaactcttt gctctaaaaa gattttagaa agtacaaatg ataatgtaaa aattattgta
     7501 tatggttgtg atgatgaggt cggtgtggtg tcgatccttt tgagagaatt agaaggcaaa
     7561 tcttcagatt cactaattat atacatggat gaaaataata atgtattaca tggatttatt
     7621 tctcccaatc attcattgag tgatttgtat tatggaggag gttatgaatt tcagtacaat
     7681 aaagaaacga aggaaatgga attaataaat cttgattttg gaaaaattag aaccattcct
     7741 gatcgtagtt tgatgcacgt tcgctcaagt tcaaaatcga atactcccac agtgaccgac
     7801 ttattccagt tcttaaccaa taagtatttc ttaccaaacg tatatgtcat gacaaaggat
     7861 cagtctttct ggcaaatgtt tttttcttat aaagaaaata aaaactatat aataaaaaaa
     7921 cagatggact gatttgtgcg ttgatccgtc cgtgagattt atttcttaag gtggctgtta
     7981 atctctattt cataaattca aaaataacat caagtaaaga atcaaaaaag ccaccttctt
     8041 ttgcttcatt tttcagaaaa gaataatcgt gttcgtcctt gatttctttt tctcttttat
     8101 aaaccttgtt atcagggact tcctgtaagt ggccacctgc tttaatccac atggcgagtt
     8161 tctgtaattc gccagcctct aaaaaaacgc catgatctcg acaacgatca ataaccactc
     8221 cagagacttg cccgtagttt tgctggttca ttaatttccc gcaaacaggg catggtttat
     8281 aaaaagtagc gttttccatt gctttgcctg caatagatct taatgcagaa aggtgagaat
     8341 gattcataga gcgtacgcca tttgaatatt cttttaagaa caagtctaat tctccgatat
     8401 caaaaaacag acctaaacat ttaggacaac gttctaaata aaaaggcttt tcatctctcg
     8461 tgatattgag gacatccatt tctattttac aattaggaca aggtcttttt ttctttggca
     8521 gattagaagt gtagtaatga cgtccagaca aatcgatatc aacacttgac ttacaataac
     8581 cacaaataga agagccgatt aataaagggg ctccacagtt tttacaacga gccattttat
     8641 ttctctggaa ttggaggagg cgtttgattc aacaaagagg aaagttcagg cctacttttt
     8701 gctgcgctcc aattgtccat tccctgtttc cagacaagag tctcagccgt taaattattt
     8761 ttttgaaata aagaggaaag agcttctaaa gaatgaggcc cgtttttttt cccgttaata
     8821 gccacccacc ataaagaaga accaggaatt ggtggtgggt tcgatacaga ttgcgtcatc
     8881 tgctgggcca tacctattcc cataccaaga ccaatgccag cagaagcttc ccctgaatga
     8941 gttgcggcag tttctgcagc tgttgccatt tggtagcgag tataatccat ttctccaacg
     9001 acgcccatgc ttgatttttt atcaatgctt cgttctgctt cttctggaag agaaatgttt
     9061 tcaataagta attgagagca agaaagacca tagcctttaa tttctgttgc gatttttttg
     9121 gagatgccta cagagagggt gtcatagttt tctgcgagtt gaaatacgcc tattttactt
     9181 tctccaatcg catcggcaaa ggccgctaca attaaatttc gaatttgttc agaaatgatg
     9241 gccattgaaa attctttctt agagcccaag acttctttaa gtaatagaag tgggtctgtg
     9301 gattgtaata tatatgtgcc gtaggctcga agacgaacag ggcctatttc tggatctttt
     9361 aaaatgatcg gatttcgagt gccccacttt tgatctgtaa aacgtctagt gctaataaag
     9421 tacacttctg ctttgaatgg gctatcaaaa ccatacttcc aacctttcag tgtcgataga
     9481 ataggcaagt ttttagtgtt aagagtatac attcctggtt gaaaaacatc tgcaactttg
     9541 ccttcgttta cgaatatggc cacctgacct tcacgaacag tcagcttagc gttgttttta
     9601 atttcatttt gatatcgagg aaatctccat aataaagtat cgcttccatc ttcaagccat
     9661 tcaatgatat caataaattc gccaatgatt ttatctagta aactcataat aaaccactct
     9721 ttaaaaagtt tctaataaaa tacattagaa ctggcctaga tgttaattct ttcgctagct
     9781 ttaattatta gacaagactt cgttctctag tctaaccatg ggtagttaaa aaaaaaagaa
     9841 aggttattga ttttgaattc agttaatgga cgcgtgaaca cttgcactat agttgagcca
     9901 tgcttatcta gtcgtgtagt ttctctggaa ataaaaaatt caaagtgatg aaaaccactt
     9961 tgaataaata acttgaaaac gtattctttg acttctaaat gaattaagtt tctggatctt
    10021 gtcttttttg acaagacaag cctttgtata aaaaggcagc gagtgtatcc agatactttg
    10081 gaacatcaat tttcattttg tcaaaatgat caaatgagtg agcataaaca gtcatttcat
    10141 gaagagacat cataatcata cgagaagtga tatccatatc atcaataaaa aactgatttc
    10201 gatctatacc gtattgtagt actcgttcca caatacgttt cgcatgaaca agaacctggt
    10261 caatataaaa ctgagttaat tcaggaaaat tattggcttc tgcggtaatc aattttggta
    10321 tgccagcaca agattcgctt tccatgagat tccaccattg gtgagtgagc tcataaagta
    10381 atttttcagg ggtgccagta taattatcca aaagttcgtt actataagat aagacagggc
    10441 tcatggcggc atgaataaca gccttaaaga gagcttcttt attttcaaag taaacgtaaa
    10501 tagttcctgg ttgtactcca gcacggcgag cgacttcagc tacttttgca gcagaaaaac
    10561 cttgttccac aaaaacattt agggccgcag caagaatctc ttgaggtcgg atttcttttt
    10621 tacgttgcca cggacggtgc gttttaatct cttcggtttc gtcgttaacg gagttttccg
    10681 gaggtaaagc atctgtattc atcatagcga tctcttattg aatttggatt agaaatataa
    10741 aaaaaaaata ataaatcgta atttcgaaaa aaaagtaacc taaaaaacaa gaaatcactg
    10801 attaggcatt tttttccatt ctttcgagag taaaaagaca ccaagaagag cagccaaggt
    10861 atcggaaata ggtgcagcca taaaaacacc tgaaaggcca aagaaatttg gaataattaa
    10921 taatagggga attaaaatca aaatttgacg actcatattt aatataatag agcgccctgg
    10981 tttccctgtc gcttgaaaat agtttccaca gatcattcct acgcctatgg caggtaaacc
    11041 aagtaaaaag attttcatcg aatgagtggc tagttcttga agctcacgat tattagatgc
    11101 aaatggttgc acaagattag gagcgaatac ttgtatcaga atccatgcta tagataagaa
    11161 ggatacagca agaacgaacg catacttcaa agcggatttt actcttgagt aatttttggc
    11221 accgtagtta aatccaacaa taggctgaat tccttgaaca aatccgagaa gaggcataat
    11281 aatcatcatg gcgatactat taataactcc aaaagccgaa atagctaagt ctcctccata
    11341 gacactttgg tttccgtaag tggcaagact tttattcaaa atgacattca ttagactatt
    11401 acaaatctgc atcatgctca tgggaaggcc aaagattagt aggcgacgga caaagggaaa
    11461 ttgcaagtgc atgaatcgcg attggatttt aataggtgta tttttcttaa agaaaaacca
    11521 catgataaac aagaaagaaa taaattgaga taaaacggta gcccaagcgg cgccagcaat
    11581 tccccaatga aattttataa taaaaagcca gtcaaaaaaa acattacaaa atgcaccaat
    11641 gagcattcta aacatggctg tttttggatg ccccatggaa cggataaaat tattcattcc
    11701 agggttaatt gtttgaaaga aaacaccaca taagataatg ctcatataag ttttggcata
    11761 aggtaaagtg acttcacttg caccaaaaac ttttaaaaga ggttctagaa aaatttgtcc
    11821 taaggcaaag acaattacgg acataatagt taaaagaact aaggaatgat ttaaaaccat
    11881 tgccgcttgt cgatactttt tctggcctaa tttaagtgaa aataaggtat ttccaccaac
    11941 gccaattaaa attgacattg ccataataaa aagagaaata ggaaaacaaa gtgtaattcc
    12001 tgtaatgcct aagtatccaa cttcttctgc aacaaaaaaa cgatctacta cattatagag
    12061 ggactctacg ctcatactta taatagaggg cactgaaaaa actgcaaaat cagttttgga
    12121 atgcttaaat agcctataga gttatatcga gctgttgttg tattggacat gtggtccaaa
    12181 gttagaaaat aatatgggat atggaaatta cattactat
//
LOCUS       DAOOBB010000044        12166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_6687_length_12166_cov_4.843118, whole genome shotgun sequence.
ACCESSION   DAOOBB010000044 DAOOBB010000000
VERSION     DAOOBB010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 12166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12166
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6687_length_12166_cov_4.843118"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PLT31_03460"
     CDS             <1..189
                     /locus_tag="PLT31_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HPW94226.1"
                     /translation="NFIAVVGVVEPLKAFEYLQKKGTIVRPQPQIGNIIRVTVGTKDQ
                     NERFLNNLKSYLIEKSDS"
     gene            complement(186..1136)
                     /locus_tag="PLT31_03465"
     CDS             complement(186..1136)
                     /locus_tag="PLT31_03465"
                     /inference="COORDINATES: protein motif:HMM:NF015763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94227.1"
                     /translation="MKNFMFLFLSLLITASFGNVSQKRFNVERTPTISVQTVVKDTVS
                     VEVPVQAQSSFFLKSGAWQELVSLQKDLRDQLTDYLKAMKEGDNKVISWFLLICFIYG
                     LVHALGPGHGKSVVAGYFLARRGKWWHGVSLGFSITMIHTMSAVILLFVLFLLARAMV
                     FSTFELSRMAIEKASYLLVVLTGLILIGASIFNRIKIKKEKSSDSIIETQASWKELLA
                     ISFITGIVPCPAVALVVFFCLLQGLYGMALLGALAIGFGMAFTNILFGVGTIFLRQGI
                     DHGAHKIGFLTQNVNMIVSLCGGILVSITGLLLFFSVSGP"
     gene            complement(1136..1720)
                     /locus_tag="PLT31_03470"
     CDS             complement(1136..1720)
                     /locus_tag="PLT31_03470"
                     /inference="COORDINATES: protein motif:HMM:NF017985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1007 family protein"
                     /protein_id="HPW94228.1"
                     /translation="MLKPIIWICLFFSVFLYAHPHIFANVFVSIEFEPSGEIIIKNRW
                     VFDELYSAAKLDVVDLNEDGKLSAEEWYEIERSVVQSAQRFNYYNYLLVGPKFIASDG
                     YKNFEIKKEGALLVIYFDLNFNLKAQKEYTILTFALKDLSNYIQFDLDMKKVKVQAPS
                     NIEVEYFVDVLDGLTMFNAFPPTTEALFLRYREK"
     gene            complement(1833..2969)
                     /gene="proB"
                     /locus_tag="PLT31_03475"
     CDS             complement(1833..2969)
                     /gene="proB"
                     /locus_tag="PLT31_03475"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HPW94229.1"
                     /translation="MSELRKKILDEARRVVIKIGSSILVDSKQGGVNIRYIASLAKSI
                     EQLSINKEVVIVTSGAVGTGMAKLGYIKKPSVIPEKQACAAVGQIELMHTYSEEFAKC
                     NMVVGQILLSAEDFRSRDRYKNLQNMIKVMLAKKIIPIINENDSLATAEIKVGDNDKL
                     SSDVALFLDADLLLIFTDEDGLFDDNPKTNPNARLLRFVPEINNEIMALAGKPDVTGS
                     AVSTGGMYSKLKSIRAVVKSGCNAFLANGKRVFPHQVFFENASGTLFTGSSKKLSSRQ
                     RWLSFVSTPRGSIVIDAGGSKALREKHSSLLPVGVISVQKHFDQGDLVNVLDESGTPI
                     ARGITKYDSETLKLVLRKKTAAVHALLGNKVPDEVIHKNDLVIM"
     gene            complement(2984..5899)
                     /gene="rapA"
                     /locus_tag="PLT31_03480"
     CDS             complement(2984..5899)
                     /gene="rapA"
                     /locus_tag="PLT31_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HPW94230.1"
                     /translation="MSIFKPGQRYISVHEPELGIGRVQAIQNKNIIFEFPAVGQSRIY
                     RNHAAPVERYILNVGETAKNEKGVSFLIESVENIADLMVYRGRGGREMNEADLMSKAA
                     ARPADLFSALLKGKISDLKEFKLREKALMLSTEWQSSTVRGMIGPRVDMIEHQYYLCH
                     RACSSSELPRLMLSDEVGLGKTIEAGMIWHALRARGRVVRSLILVPESLKHQWMVEMK
                     RRFNHIFTLVDEGFLRALFSGNDKPNPFLQANEVICTVELLMDHPALIEDLLKVKWDL
                     TIVDEAHHLVCEDGFTSKEYMLVNAIVSRSKGVLLLTGTPLQLHPESHFNRLRMLDPA
                     RFSDFNEFIKDQERYRKLANDLAKLPTDSDSHLSWDDLYELVPKKSSIREWLEKENSK
                     AMSAGEWMRRIVDALGTGSVVFRNTRKGVGGFPKRHLDAIELAPNPKYREWVALVANK
                     DPDHSTDIQENGLLITNYKEGWNQDERFLWLMSFLKEHQQEKVLLICEDIAVVSALDS
                     LLEEYYGKGAYAVFHENLSILARDQAAAWFSEPEGANLLIASEIGSEGRNFQFAHHLV
                     LFDLPLDAALVEQRIGRLDRIGQNRDIIIHVPYVKDSAQEVMFRWYHDGLGAFTSPLM
                     SGGELFLKYTDALIEALDNPNDNLDSFVKNVIPKVHEDSLELRKNIEKGRDRLLEFNS
                     RDPETARKIIEEIQRLDKDVSLFSFVYDVLTSHGVEIEKSAIPNSFVFSAGPQVESGT
                     IPGMPTSSMYAQKEEEEREVASSTLTVTNSRTQAKLREDIEFLSWEHPLTQGAIDLAT
                     AMGKGSTTCSIWENSGLRGLMMQYNFVLEPSVLAEWGFSDIAGPAVLRVLIDGAGENR
                     TDLLASLDEGSSKTAPIPQGNAAVATKIRYFASEGFAIAKRVVSIQVSEISEKASLAV
                     ESRSEQEYQRKQHLLSLRGKAESDTSLPILRKNIQERKKAAAFPQLRLDAVRLIVCR"
     gene            5963..6712
                     /locus_tag="PLT31_03485"
     CDS             5963..6712
                     /locus_tag="PLT31_03485"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HPW94231.1"
                     /translation="MNWFYIDKSIKTGDRRQGPYTIEEMRELAAQGTIEKTSLVWHNG
                     LENWITWEKSDGFISEEKVNEMIQQTIAEILLEKKKVQKNAAGFGIRAMAFLIDFIIL
                     SLLGSLIVYFLSALHWIDLETINVIMEKYLSNPLSPDFAKEFLEAKGVSTFVSIWFLI
                     QAFYSIYFHTKYAGTLGKLLLHLKVENADGTKMNLAKAFLRYVYSIITQLTLAFYGIG
                     YLLALIDPKKRALHDFLANTRVVYSDFDKDK"
     gene            6719..7987
                     /gene="murA"
                     /locus_tag="PLT31_03490"
     CDS             6719..7987
                     /gene="murA"
                     /locus_tag="PLT31_03490"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPW94232.1"
                     /translation="MDRYLVSALKQPLHGEVEISGAKNAVLAVMAAALLADGVSEITN
                     APHLKDMKTMSDVLRVIGCRIDGGSHTLHIDTRGVNHLEAPYELVKTMRASFYVLGPL
                     VSRFGRCRVSLPGGCAWGPRPVDLHLKGLEALGATINVTHGYVEALCEGRLKGGTFHF
                     PISSVGATVNVLMAATLASGKSTLHNVAIEPEIDALVDYLIQMGAKIEGRGTRTLVIE
                     GVEKLNPGSGATIPDRIEAGTYLIGAAMTRGCIKTTKMNPSHIEALLSSLREMGCDVS
                     VGDSWVEVNAYQKKLKPISVTTLPHPGFPTDLQAPMMALLATIDGVSVIQDTVYNDRF
                     THIAELQRLGADIQLTGNTAHVRGGKSLSSAPIMASDLRAGAALVLASLLAEGETSIS
                     RIYHIDRGYENFEEKMRSIGAQIQRTQEEG"
     gene            complement(7992..9131)
                     /locus_tag="PLT31_03495"
     CDS             complement(7992..9131)
                     /locus_tag="PLT31_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94233.1"
                     /translation="MSWPLMRIAGILFLFLVVNIFADEQTSLYRRALEAESYGDIVSA
                     VTLLEEALLTEGEYTEEILQILKQYYNVLGIEFIDAQKQRPWRFGLDFDAIGIRYTEK
                     VNGLNSREWIGEAFSVLSLGYDWHRKQIKHSLEISFLSDLFWKSDSSSLDTNDWVLSP
                     SIQYILMSKSFALSTGIDFTINEKDGFNPDVFFFFEKYAFKKGSRWFALDAFAFVNKI
                     LEMRYGFNLSWNQIQKKGLSISTSLGAQLNVDSTVFIQWIYDDNFFDGFDPPYLDVSY
                     YQGVKWGPTLKVKTSYQFENPIGIEIKGRIAPSFLLNGKDSASVSFIEGGFGGYLFVK
                     WDKIRVHVGVEEFLGYYLERPEIWKDYIPKTTLFTEFKIGTSIKF"
     gene            complement(9116..9538)
                     /locus_tag="PLT31_03500"
     CDS             complement(9116..9538)
                     /locus_tag="PLT31_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94234.1"
                     /translation="MKRTYYFFLISLLASLSFGADDKVLPSPQISTPADKPVSSKNWT
                     LGEKESSDWQKMRMERQKAREEILSKIRESSGQEKEQLRRRLSKNRNHDARFDGDSQK
                     RMKSRERTPFFERPESHNVRPMREKEFPPPCPGDCHGH"
     gene            complement(9615..11069)
                     /locus_tag="PLT31_03505"
     CDS             complement(9615..11069)
                     /locus_tag="PLT31_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPW94235.1"
                     /translation="MSIDTLQATEELLDTLIHLLDESNPDDLLPKILKSATQILHADA
                     SVLEIPGDETLLLFYPEKSPISLSAVKKAKGENRVILWNQLEDDDVDLSQSIVQNQLT
                     SIMVAPFRTPQSESGYLYLQRKARTDSFSEQERLLFESFVTVCEKLAFTAYDRLREKE
                     SLDVLKGICRKGGIIYGSEPMVNLMALAEKLAALPLPVIIRGETGTGKELFARWLHSE
                     SPRRDMPFVAINCGAIPENLIESILFGHVKGSFTGAIENRKGVFEQAEGGAVFLDEIG
                     ELPLSMQVKLLRVLQEKHITRVGDNREIPVNVRVISATHVDLEEAVKEKKFREDLYFR
                     IQVMPVEIPPLRNRGQDVILLAESFLEKYCAEYGRGRFRLSRASEKALLSYSWPGNVR
                     ELENKIQKSLIQAVRGVISPEDLGLENVSEQVKNSPRTLREARESAEKTVIHQALLDS
                     KGNLTLAATILGIDRKVLREVMERLKIRKEEYKN"
     gene            complement(11066..>12166)
                     /locus_tag="PLT31_03510"
     CDS             complement(11066..>12166)
                     /locus_tag="PLT31_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPW94236.1"
                     /translation="LMHAGGEAFVYRARYKKRETVALKIYKEGLHFNPDFYLALKKNK
                     HANVASIFDYGFYSDRFWSCSEYIEGIASSAVAPFSVPIALHLLKGIVSGLSFLENHH
                     LSHGDLSPDNILITKEGSPVLIDGGIQGVGHLGYAAPERFENAESTVKSDLFSLGILL
                     YFWLTNSLPYEKEEYDSIVEEILHIESSKISVLLHLKNSFSIEEIGTLDPIWKGLLQL
                     NSEDRFSSFEELEEHLEIALLKLSKQAICLEKSYKNWLLFLDEKIVEQEVALDQKEVE
                     SLNGFSSHSIVKKPFLRVWIFSFILFLLFIISLLFIFGEERKSQNVLESGEQMLERVR
                     SREIPLEPKSKNKGITIENVLRPIDILNKDSL"
BASE COUNT         3964 a         2527 c         1972 g         3703 t
ORIGIN      
        1 aactttattg ctgttgttgg tgttgtagaa ccactaaaag cctttgaata cttacaaaag
       61 aagggaacaa ttgttcgtcc tcaaccacag atcggaaata tcattcgagt taccgttggc
      121 acgaaagatc aaaacgaacg tttcttaaat aacttaaaat cttatttaat agaaaaaagc
      181 gattcttaag gcccagaaac gctaaagaaa agtaataagc cagttatact gactaaaata
      241 cctccgcaaa gagacacaat catattcacg ttttgtgtta agaatcctat tttatgggca
      301 ccgtgatcaa tcccttgcct taaaaagatc gttcctacac caaatagaat attggtaaac
      361 gccattccaa atcctattgc tagtgcaccc aaaagagcca ttccgtataa gccttgtaat
      421 aaacagaaaa agacaaccaa agccacggct gggcaaggga caatacctgt tataaaggat
      481 atggcaagta attctttcca agaagcttga gtttctatta ttgaatcaga tgatttttct
      541 ttttttattt taatccgatt aaaaatagaa gcccctatta atataagacc tgttaaaacc
      601 actaacaaat aacttgcctt ttctatcgcc attcgactta actcaaaagt agagaaaacc
      661 atagcacgag ctaataagaa tagaacaaac aataaaataa cggcactcat tgtatgaatc
      721 attgtaatcg aaaagcctaa actgactcca tgccaccatt tccctcgacg tgctaaaaaa
      781 taccccgcca caacagattt tccatgacct ggacctaacg catgaactaa accatagata
      841 aagcaaatca gaagaaacca actgatcact ttgttatcac cttctttcat tgcttttaaa
      901 taatctgtta actggtcacg caaatctttt tgtagtgaaa ccaactcttg ccaagcacca
      961 ctctttaaga aaaaagaact ttgtgcttgt actggaacct ctacagaaac ggtatcctta
     1021 accaccgttt gaacacttat tgttggagtt cgctccacat taaaacgttt ttgagaaaca
     1081 ttaccaaaag aagcagtaat gagtaaagac aaaaaaagaa acataaaatt tttcatcatt
     1141 tctccctata ccttaaaaaa agagcttctg tggttggcgg aaaagcatta aacatagtca
     1201 aaccatctaa gacatctaca aaatattcca cctcaatatt tgaaggagct tgaactttca
     1261 cttttttcat atccaaatca aattgaatgt aattagacaa atcttttaat gcgaatgtta
     1321 aaatggtgta ttctttctga gccttcaaat taaaattaag atcgaaataa ataactaaga
     1381 gagccccctc tttttttatt tcaaaatttt tataaccatc agaagcaata aatttgggac
     1441 ccaccaataa gtaattataa taattaaaac gttgagccga ttggactaca gaacgttcta
     1501 tttcatacca ctcttctgca cttaattttc catcttcatt taaatccact acatctagtt
     1561 ttgcagcact atataattca tcaaaaaccc aacgattttt aataattatt tctcctgaag
     1621 gctcaaattc tatcgataca aaaacgtttg caaatatatg gggatgcgca tataagaaaa
     1681 cagaaaaaaa gagacaaatc catattattg gtttcagcat aaaaacaaat tacaatttta
     1741 ttttttaaac cagataatat tttttgatca aattaaaaac cttgaaataa tcagcttatt
     1801 tgtattaaaa agaatagtgt atttgttctt aatcacatga tgactaaatc atttttatga
     1861 ataacttcat cagggacttt attgcctagt aaagcatgaa cagctgctgt ttttttccgt
     1921 aaaactaatt ttaatgtttc gctatcgtat ttagtgattc cacgtgcaat tggagttcct
     1981 gattcatcta acacattgac caaatcccct tgatcgaaat gtttctgaac agagatcaca
     2041 cccacaggca aaagactgga gtgcttttct cttaatgctt ttgagccacc agcatcaata
     2101 acgatagaac ctcttggtgt acttacaaaa gaaagccagc gttgacgact gctaagcttt
     2161 ttagaagacc ctgtaaaaag agttccagaa gcattttcaa aaaagacttg atgtggaaaa
     2221 acacgttttc cattggctaa aaaagcattg catccacttt tcactactgc acgaatagac
     2281 tttaatttag aatacatgcc accagtgctc accgctgaac ctgtaacatc tggcttacct
     2341 gcaagcgcca taatctcgtt attaatttct ggaacaaacc gcaataaacg agcattagga
     2401 ttcgttttag gattatcatc aaataagcca tcttcatcag taaaaatcaa caataaatca
     2461 gcgtctaaaa ataaggccac atcacttgaa agtttatcgt tatccccgac ttttatttct
     2521 gctgtagcta atgaatcatt ttcattaata attggaatta ttttttttgc aagcatgact
     2581 ttaatcatgt tttgcaaatt tttataacga tcacgagaac ggaaatcttc tgctgaaaga
     2641 agaatctgcc ctacaaccat attgcatttt gcaaattctt cactgtaagt atgcattaat
     2701 tcaatttgcc caacagccgc acaagcctgt ttttcaggta ttactgatgg ctttttgata
     2761 tagcccagtt ttgccattcc agtaccgaca gcaccacttg tgacaatcac cacttcttta
     2821 ttaattgaga gctgttctat agacttcgca agagaagcaa tgtagcgaat attgactcca
     2881 ccttgtttag aatcgacaag aatactcgat ccaattttga tcaccacacg acgagcttca
     2941 tctaatattt tttttcttaa ttcactcata ccaacaaaac ctatcaacga caaacaatta
     3001 atcgtacagc atctaagcgt aactgaggaa aagcagctgc ttttttacgt tcctgaatat
     3061 tttttctcaa aataggtaac gaggtatcac tttccgcttt accacgtaaa gaaagcaaat
     3121 gctgttttct ctggtattct tgttccgaac gactttctac cgctagcgag gctttttctg
     3181 atatttcaga aacctgaatg gaaaccacac gcttagcaat agcaaatcct tcgcttgcaa
     3241 aataacgaat ctttgtagca accgcagcat ttccctgagg gatcggggcc gtttttgaag
     3301 acccttcatc tagacttgca agcaaatcag ttctattttc acctgcacca tcaattaata
     3361 ctcgaagtac agcgggccct gcaatatctg aaaagcccca ctctgctaaa acagatggtt
     3421 cgagaacaaa gttgtactgc atcattaatc cacgaagccc tgaattttcc caaatagaac
     3481 aggtcgtaga cccttttccc atcgctgtcg ccaaatcaat agcgccttgt gtgagcgggt
     3541 gttcccaact taaaaattca atgtcttcac gaagcttagc ttgcgttctt gaattggtga
     3601 cagtcaaagt cgaagaagcg acttctcttt cttcttcctc tttttgcgca tacatggatg
     3661 atgttggcat tccaggaatc gtacctgatt caacttgagg accagcagaa aaaacaaaac
     3721 tatttggaat agcactcttt tcgatttcca ctccatgaga cgtcaagaca tcataaacaa
     3781 aactaaacag cgaaacgtct ttatcgagtc gttgaatttc ttcaataatt ttacgagccg
     3841 tttctggatc tcttgagtta aactctaaca atcgatctcg acccttttca atattcttac
     3901 gaagttcaag actatcttca tgtactttag ggatgacgtt tttcacaaaa gaatccaaat
     3961 tatcatttgg attatctaag gcttcaatta aagcatctgt atattttaag aagagttctc
     4021 caccactcat taatggcgac gtaaatgctc ctaagccatc atgataccaa cggaacatga
     4081 cctcttgagc agagtctttc acataaggaa catgaataat gatatctcga ttttgcccta
     4141 tacgatccaa acgccctatg cgttgttcaa ctaacgctgc atctaatggc aaatcaaata
     4201 aaaccaaatg atgagcaaac tggaagttac gaccttcaga tccaatttct gaagcaatta
     4261 acaggtttgc accttctggt tcagaaaacc atgctgcggc ttggtcacga gcaagaatag
     4321 atagattctc atggaatacc gcataagcac cttttccgta atactcttct aacagagaat
     4381 ctaaagccga aacaacagca atatcttcac aaatcaaaag cactttttct tgttgatgct
     4441 cttttaagaa agacatcagc cacaaaaagc gttcatcttg attccaacct tctttatagt
     4501 tggtgatgag aagaccattt tcttgaatat cagtcgaatg atctggatcc ttgttcgcca
     4561 ccaaagccac ccactctcta tactttggat taggtgcgag ttcaatcgca tctagatgac
     4621 gcttaggaaa tccacccacc ccttttcgag tatttctaaa aacgacagag ccagttccaa
     4681 gagcatctac aattcgacgc atccattctc cagcagacat tgctttagaa ttttcttttt
     4741 caagccattc tcgaatagat gactttttag gaacaagttc ataaagatca tcccaactta
     4801 aatgactatc tgaatccgtt ggtaacttgg caagatcatt tgccaatttt cggtatcttt
     4861 cttgatcttt aataaattca ttgaagtcag aaaaacgagc tggatcaagc atgcgtaaac
     4921 gattaaaatg cgattcaggg tgcaactgta aaggcgttcc cgttaaaagt aaaacccctt
     4981 tagagcggct aacaatagca tttacaagca tatattcttt actagtaaaa ccatcttcac
     5041 atactaagtg atgagcttca tctacaatag ttaaatccca ttttaccttt aataaatctt
     5101 caattaaagc gggatgatcc attaataatt ctacagtaca aatgacttca tttgcttgta
     5161 aaaaaggatt tggcttatca ttaccactaa acaatgctct taaaaagcct tcatccacga
     5221 gtgtaaaaat atgattgaag cgccgcttca tttccaccat ccactggtgt ttcaaagatt
     5281 cagggactaa aataagagaa cgaaccacac gcccacgcgc acgtaaagca tgccaaatca
     5341 tgcctgcttc aatcgttttt cctaaaccca cttcatcgga aagcatcagt cttggtagtt
     5401 cagaagaaga acaagcccta tggcaaagat aatactgatg ttcaatcata tcgactcgag
     5461 gtccaatcat acctcgaaca gtggacgatt gccactcagt agaaagcatt agagcttttt
     5521 ctcttaactt aaattcttta agatctgaaa ttttcccttt tagcaaagca gagaataaat
     5581 ccgcagggcg agccgccgcc ttagacatta agtctgcctc attcatttct cggccgccac
     5641 ggccgcgata aaccatcaaa tcagcaatat tttctactga ttcaatcaaa aaagaaacgc
     5701 ctttttcatt tttagccgtt tctccgacat tcaatatata acgttccact ggtgccgcat
     5761 gatttctata aatccgactc tgccctaccg ctgggaactc aaaaatgatg tttttatttt
     5821 gaatagcttg aacacggccg attcctaatt ccggttcatg tacactaata taacgctgac
     5881 ctggcttaaa gatactcatg agggcataaa ttagcttttt tctttttaaa aagaggaaga
     5941 tgtttctaaa ttagaccttg ttatgaattg gttttatata gataagagta ttaaaacagg
     6001 tgaccgacgt cagggtcctt ataccattga agagatgcgt gagcttgccg cacaaggcac
     6061 tatcgagaaa acttccctcg tttggcataa tggccttgaa aattggatta cctgggaaaa
     6121 atctgatggc tttattagcg aagaaaaagt aaatgaaatg attcagcaaa ccattgctga
     6181 aattttactt gaaaagaaaa aagttcagaa aaatgctgct ggatttggca ttagagccat
     6241 ggctttcctt atcgatttta tcatccttag tttacttggt agcctaatcg tttatttttt
     6301 aagtgccctc cattggattg atctagaaac gatcaacgtc ataatggaaa agtacttgtc
     6361 aaatcccctt tctcccgact ttgcaaaaga atttttagag gccaaaggag tctctacatt
     6421 tgtctccatt tggtttttga ttcaagcatt ttattccatt tattttcaca ccaaatacgc
     6481 tgggacttta ggtaaacttc tacttcatct aaaagtggaa aacgctgatg gaactaagat
     6541 gaatttagcg aaagcttttt tacgttatgt ttatagcatt ataactcaac tcactcttgc
     6601 tttttatggt attggctatc tcctcgcttt aatcgaccca aagaaaagag cattacatga
     6661 tttcttagct aatactcgtg tggtttacag tgatttcgac aaggacaaat aaaacattat
     6721 ggatcgttat ttagtctcag cattaaaaca acctcttcat ggtgaagttg aaatttctgg
     6781 tgctaaaaac gccgtcttag ccgtaatggc agctgctctt ctcgccgatg gtgtttctga
     6841 aatcacaaat gctccccacc taaaagacat gaaaaccatg tccgatgtac ttcgtgtcat
     6901 tggatgtcga attgatggtg gatcacatac tttgcatatt gatacaagag gtgtaaatca
     6961 tcttgaagca ccttatgaat tagttaaaac catgcgtgca agtttctacg ttctagggcc
     7021 tctggtaagt cgttttggac gttgccgagt gtctcttcct ggtggctgcg cttggggtcc
     7081 tcgtccagta gaccttcatt tgaaaggcct tgaagctctt ggagcaacta ttaacgtcac
     7141 ccatggttac gtcgaagctc tttgtgaagg gcgcctcaaa ggaggaacgt tccatttccc
     7201 aatcagtagt gttggagcaa cagtcaacgt tttaatggct gccactctag cctcaggtaa
     7261 aagcactcta cacaatgtcg cgatcgaacc agaaatcgat gctcttgtag attacttaat
     7321 tcaaatggga gctaaaattg aaggccgtgg tacaagaact cttgtcattg aaggtgttga
     7381 aaaactaaat ccaggatctg gagccaccat ccctgatcgt attgaagcag ggacttattt
     7441 aattggtgct gcaatgactc gtggttgtat caaaacaacc aaaatgaatc cgtctcacat
     7501 tgaagctctc ctcagtagtt taagagaaat gggatgtgat gtttctgtcg gtgattcttg
     7561 ggttgaagtc aatgcctatc aaaaaaaatt aaaaccgatc tccgtcacca cattacctca
     7621 tcctggtttt cctactgatt tacaagctcc catgatggct ttacttgcca ctattgatgg
     7681 cgtgagtgtg attcaagata ctgtttataa cgatcgattt acccatattg ctgaattaca
     7741 acgtttaggt gctgatatcc aacttacagg caatacagct catgtacgtg gtggcaaaag
     7801 tttatctagt gctcctatta tggcttcaga cttaagagct ggtgcagcac ttgttttagc
     7861 atctcttctt gctgaaggag aaacatccat cagtcgtatt tatcacattg atcgcgggta
     7921 tgaaaacttt gaagaaaaga tgcgttccat aggagcacaa attcaaagaa ctcaagaaga
     7981 gggttaattc gtcagaattt aatgctcgtc cctattttaa attctgtaaa taaagtcgtt
     8041 tttggaatat agtccttcca tatttcagga cgttccaaat aataccctaa aaattcttct
     8101 actcctacat gtactcgaat tttatcccac ttaacaaata aatacccgcc aaacccacct
     8161 tctataaaag aaacggaagc agaatctttt ccgttcaaca gaaacgaagg agcgatacgt
     8221 ccttttattt caattcctat tggattttcg aattgataag acgttttaac tttaagcgta
     8281 gggccccatt ttacgccttg ataataactc acatcaagat atgggggatc aaaaccatca
     8341 aagaaattat catcataaat ccattgaata aaaaccgttg aatccacatt cagttgagcg
     8401 cccaaagaag tagatatcga aagtcctttt ttctgaattt ggttccatga taaattaaaa
     8461 ccataacgca tttctaatat tttgttcaca aaagcaaatg catctaaagc aaaccaacgt
     8521 gaaccctttt taaaggcata tttttcaaag aaaaagaaaa catcgggatt aaacccatct
     8581 ttttcattga tagtaaaatc aatccccgtg gataaagcaa aagatttaga catcaatatg
     8641 tattgaatcg aaggagataa aacccaatca ttggtatcta aagaagaaga atctgatttc
     8701 caaaatagat ctgataaaaa agaaatttct aatgaatgtt ttatttgttt gcgatgccaa
     8761 tcataaccta atgaaagaac ggaaaaagct tccccgatcc attctctaga atttaaacca
     8821 tttacttttt ctgtataacg aatcccaatg gcatcaaaat ctaatccaaa acgccaagga
     8881 cgttgctttt gagcatcaat aaactcaatt cccaaaacat tatagtattg ttttaatatt
     8941 tgaagtattt cttcagtata ctccccttct gttaaaagag cttcttctaa taaagtgacg
     9001 gcactaacaa tatcaccata agattccgct tctagagcac gtcgatacaa agatgtttgc
     9061 tcatctgcaa aaatattaac aacaaggaat aaaaatagta tacctgcaat acgcattaat
     9121 ggccatgaca atcccccggg caaggcggag gaaattcttt ctctcgcatt ggcctcacat
     9181 tatgagactc tggacgttca aaaaaaggcg tacgttcacg agatttcatt cttttttggg
     9241 aatccccatc aaagcgggcg tcatggttcc gattttttga aaggcggcga cgaagctgtt
     9301 ctttttcttg tcccgaactt tcacgaattt tagacaaaat ttcttctcga gctttttgcc
     9361 tttccattct cattttctgc caatctgagc tttctttttc acctaaagtc cagttttttg
     9421 aagaaacagg cttatcggcg ggggtagaga tttgaggtga aggcaaaact ttgtcatcag
     9481 caccaaacga taatgatgct aacaaagaaa tcaagaagaa ataataagta cgtttcatat
     9541 gatatctcta acaaaaacta tgccaacaaa aaaagttaat atatccttct ataaaaaaac
     9601 tgaatattaa tatactaatt tttgtattcc tcttttctta tttttaatcg ctccataacc
     9661 tctcttaata cttttcgatc aataccaaga atggtagcag ctaacgtcaa attcccctta
     9721 ctatccaaaa gggcttgatg aattacagtt ttttcagccg attctcttgc ctccctaagc
     9781 gttcttggtg aattttttac ttgttcagaa acattttcta accctaaatc ttctggtgaa
     9841 atgacaccac gaacagcctg aattaaggat ttttgaattt tattttctaa ttcgcgtaca
     9901 tttcctggcc aagaataact tagcaaggct ttttctgaag cccgacttaa tcggaatcgc
     9961 ccgcggccat attccgcaca atatttttcc aaaaaggatt ctgctaaaag aatgacatct
    10021 tgacctcgat ttcgtaaagg aggaatttcc acaggcataa cctgaattct aaaataaaga
    10081 tcttccctaa actttttctc ttttacagcc tcttctaaat caacatgagt ggctgaaatc
    10141 accctcacat tgactggaat ttcacgatta tcaccgactc tggtaatatg tttttcttga
    10201 agcactcgta ataattttac ttgcatcgaa agaggaagtt cccctatttc atctaagaaa
    10261 acagctcctc cctccgcctg ttcaaacacc ccttttcgat tttctatagc ccctgtaaaa
    10321 gagcctttta catgaccaaa caaaatagac tctatcagat tttcaggaat agcaccacag
    10381 tttatcgcta caaaaggcat gtctcgtctc gggctctcag aatgaagcca acgagcaaaa
    10441 agttccttac cagtccctgt ttcccctcga ataataacgg gtaagggaag agctgctaac
    10501 ttttctgcaa gagccattaa attaaccatc ggctctgagc catatataat gccaccttta
    10561 cggcaaatcc cttttaaaac atctagggat tctttttctc ttaatcgatc ataagccgta
    10621 aaagcaagct tttcacaaac agtgacaaaa gactcaaata aaagacgttc ttgttccgaa
    10681 aaagaatctg ttcgtgcttt tcgttgtaaa tataaataac cagactcact ctgtggcgtt
    10741 ctaaatggag ccaccataat acttgtcagc tggttttgta caatcgattg agaaagatcc
    10801 acatcatcat cttctaattg attccaaaga atgacacgat tttctccttt cgctttttta
    10861 acggcagata aagaaatggg tgatttttca ggataaaata aaagaagagt ttcgtctcct
    10921 ggaatctcaa gaacagatgc atccgcatgt aatatctgtg tggcagactt aagaatcttt
    10981 ggaagtaaat catctggatt ggactcatct aacaaatgaa tcaaagtatc tagaagttct
    11041 tctgtggctt gcaacgtatc tatactcata aagaatcctt attcaaaata tcaataggac
    11101 gaagaacatt ttcaattgta atccctttat tttttgattt gggttctaaa ggaatttctc
    11161 tgctacgaac tcgttcgagc atctgttctc cagattctaa aacattttga ctttttcttt
    11221 cttctccaaa tataaagaga agggaaataa taaataaaag aaaaagaata aaagaaaaaa
    11281 tccaaacacg taaaaaaggc tttttaacaa tagaatgact agagaatcca ttcagagact
    11341 ctacttcttt ttgatctaat gccacttctt gctctactat tttttcgtct aaaaatagta
    11401 gccagttttt atatgatttt tctaaacaga ttgcttgttt agaaagtttt aaaagagcta
    11461 tttctaaatg ttcttctaat tcttcaaaac ttgaaaagcg atcctcagaa ttcaactgta
    11521 ataaaccttt ccagatcgga tctagagttc ctatttcttc gattgaaaaa gagtttttta
    11581 gatgtaataa aacagaaatt tttgatgatt caatatgaag aatttcttct actatagaat
    11641 cgtattcttc tttttcataa ggaagagaat tggtgagcca aaaataaagt aatattccta
    11701 aactaaataa atcgcttttt actgtagact cagcattttc aaaacgttct ggagccgcat
    11761 aaccaagatg cccaacgccc tgaataccac catcaatcag tactgggctt ccttcttttg
    11821 taatcaaaat attatcagga gaaagatccc catgagaaag atgatgattt tctaaaaatg
    11881 aaaggcctga aacaattccc ttcaataaat gtaaggctat aggaacggag aatggggcaa
    11941 ctgcagaaga tgcaattccc tcaatatatt cagagcaaga ccaaaagcga tcgctgtaaa
    12001 atccataatc aaaaatcgaa gctacattcg cgtgtttatt cttttttaat gccaaataaa
    12061 aatctggatt aaaatgaagc ccttctttat aaatttttaa agcgacagtt tctcgtttct
    12121 tatacctcgc gcggtaaaca aaagcctcac caccagcgtg cattaa
//
LOCUS       DAOOBB010000045        11779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_6958_length_11779_cov_4.514159, whole genome shotgun sequence.
ACCESSION   DAOOBB010000045 DAOOBB010000000
VERSION     DAOOBB010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 11779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11779
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6958_length_11779_cov_4.514159"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /locus_tag="PLT31_03515"
     CDS             complement(<1..1117)
                     /locus_tag="PLT31_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94237.1"
                     /translation="MKNTRFTHLLLLLLTTATFFIACGDDKPNKPEESSSSSEASSSS
                     SSLINRLDVTYVDTLESNETANFYFEDDSTTSERALFYLGDLKAGSLIKIAAATTGIN
                     NDTIRIYEEHGALVKTIFPTPDAKGNNVYNYMLPGTGSELDSNQFITLEEGFYYLELK
                     GEIKESSHLRIFSEINEEYYSYIGDSSSVSFVTNDTLRGFFIIGSGPRKLDISIEANT
                     GISLNIDVSGSWINEYLLTEGLDTLAKDTAQIDKLLLPQKSTTFNVLLKPLSISNYLT
                     GPYAFFELITNSRTLDKGEYLAKPDSIVRPGDTLVVVRPRNDQAKYYLRQEQYIWLSD
                     LKKGDTLFISHEIEGYYSGPLYPATLTVLNADGDSLQN"
     gene            complement(1135..4209)
                     /locus_tag="PLT31_03520"
     CDS             complement(1135..4209)
                     /locus_tag="PLT31_03520"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HPW94238.1"
                     /translation="MFQKILFVVFTLFSLISAQISFPLGSEVVDVTKEPYFAKGDGKA
                     DDTEAIQRALDDHPNGDFIIYLPHGTYKISKQLSWPKSEEPTEAYRRTILQGESMGGT
                     ILILKDNARGFDNPEIPRAMIYTGIGPHPRHRNAVRDMSLRTGKGNPGAIGIRFNAAS
                     QGTVFNVKIFSGDKNGAGVAGIDMGFAENVGPLLLKNIEVRGFDIGIYTRTPFFGMTL
                     EHISLSGQRKYGIENWDQALTIRGLRSRNSVPAIYNTGEYAMLTLIDGTLEYVPNKKK
                     PAGPAIINEAGLFVRGVTTSKYKTPIKSTTPGLGEGIKGQEILEYVSENLINLCHSPK
                     ASMRVPVAEVMELPKQTPSNWVKITGDYGGTAGDGRDVSKAIQQAIDDGAETIYFPPK
                     GRYTINRDVFIRKRVRHIIGTEGRIEGTGRFIILDGTFPDLTIERFSSFGNGILHKSN
                     RLLLLKNVMVKSYEGDVDGAGDVYMEDVSVDQIKLNFQHLWARQLHMNNDKTTKIINN
                     GGTIWVLGLTAENGNTILHNKNKGKAELAGVHVISNDKAKVQPMFINDSSNISIEGLR
                     ETTIRGNAFHKIIEETRKGLTKTLYAKDLKRGASGGVNIPLYVGYIPQTGNNIAPKAE
                     TLNEQILVQPNKIRLKGIVDDDGRADGLCEVPVEWTKAKGPGRAVFLDHRSYETDVTF
                     TASGRYDLLFKAHDGQLKGTDTARVFVFDKRYSTFDHNGDNVPSGRGADAWVSEFDNY
                     TPQSNDSVLRISYNNDNSSKIYLKFDLSALPGPLSDAALQLNVKNIDKIKKPIQWNVF
                     GLKEIDKNYNFGEDKLGPEWKESELTWANAPGNLDEGGGLFNVRKGIGGGVDTRFADH
                     LGVITIDPKATLSSFLRSPSFTQFFKRKHPSGLYTLILTGTEKSSTEYQISAKEQGRD
                     FAPVLYISYFDSTKSAGGESMQGGYQLSKVNIDIYTLKCSFDLVLGYPQFVQIEVYNE
                     FGKRMMIVAEKDLESEKKLNFQFEAISFPTGKYILKIIGEAFTTQQSFYILN"
     gene            4420..5682
                     /locus_tag="PLT31_03525"
     CDS             4420..5682
                     /locus_tag="PLT31_03525"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPW94239.1"
                     /translation="MLLFLVYIFILTSFAFSEDVTEISSCEEGQLVSKIEMLELKHTR
                     TRVVERELLHQIGKAYSKTLWLKEKGRLEGLDLFAEVRLSCKKESSGLVLEYHLPEIF
                     NIIPSPAGKKTDQDGLMLGLALAHLNLGGDDIRAEIQYRTSVSPFFSSNEYALYASSP
                     WFLNQPLGWNFEFLRTSSWDNLRLFEEESYLADIDLQWFLKKPYGFLFSLAYRYLNNY
                     GYTPSVSLGFLIDSRQPNVDSRSGMYQEWLWTYNGGIANSDQNYQEYLWDSRFYFTVN
                     RFVSGASSLLRVRLGEVAFYDRFHHGGANTFRGWDPDSLCYGTHEWILNFEERIVLKE
                     RAPVSIGGVSLFYGLQWVVGVDGAFLWDKGLPTWSDYHSALYTGLHVIIPALDRIRFE
                     VGYSSDESEFKFFIGLYEKNIAQRWRSR"
     gene            5817..6419
                     /locus_tag="PLT31_03530"
     CDS             5817..6419
                     /locus_tag="PLT31_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HPW94240.1"
                     /translation="MAINYLDLPIGPKYPYEVDCVVEIGKDTNVKYEYDEHLHVFRLD
                     RCLLSSMSYPCSYGFVPSTLADDGDAIDMLIYNSASLNTGTVVTCRVVGVLDMTDGGK
                     KDYKVLGVPLYTPHPIRDIQDVDPMFLKITRNFFQYYKELEGKNVQIGEWLDAKFARE
                     KIVSAHHSYFQTQVQMPETRYQEPETDSSCDSSVIPSILM"
     gene            complement(6512..7273)
                     /locus_tag="PLT31_03535"
     CDS             complement(6512..7273)
                     /locus_tag="PLT31_03535"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HPW94241.1"
                     /translation="MKFLIAFISLCITITAIVYIKQNPKSIPLSEEGKQAIQYIDVSG
                     YHSEMFSSDEILWELQISLIGKEKEELLAKKNERIKELNSLVSSIGIDPSVLSFENYT
                     LRKQWNWDHGKKTFSNYQLTQTIKIQFHSEEKSDLLSEGLIQINDIEIVNVQYEIGEK
                     EDKLNKMAAKAFEKAKDKAEALAKTSNKRIASVISISESPISGESMILEGFLGGGSRA
                     LKSSMPGTNGPTKAKLESQLFVRFLLKDDNKKLFD"
     gene            7462..8139
                     /locus_tag="PLT31_03540"
     CDS             7462..8139
                     /locus_tag="PLT31_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94242.1"
                     /translation="MKKIILLFLLAVPLTHADIIRTKACDRGNCCNLTINDRDDSYSL
                     NCDRRNTEVILSVSDRRGRHEFNQVLRSDRGRLDRRISRQHSFRMDIREVRGRHETVV
                     VKEGPRRHETIVVKDNSRPRRRSGPARIHYEEPDCYDIGSGRFRIEYESGEVLVEGRC
                     HRRDRDGEFLFYNDFGDLWMRVNYHRDRAERIECRDRRGHWRNVRSEDDCFRDYDVRT
                     IRRARRR"
     gene            8247..8744
                     /locus_tag="PLT31_03545"
     CDS             8247..8744
                     /locus_tag="PLT31_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPW94243.1"
                     /translation="MMKSLKKEKFELIYQQYMPMVLRRCRFLLKNEEEAFDVAQDVFI
                     RILSKQDSLDLEAPSSLLWNSATRLCLNRIRNKTRRDLNRPTEDLLCQIACVRDERDD
                     YEHQYLLSKLFQSEPASSRTIAVLHFIDGMTFEETARNVGLTVGQVRYRLNTLQKKLK
                     SMEEK"
     gene            8741..9493
                     /locus_tag="PLT31_03550"
     CDS             8741..9493
                     /locus_tag="PLT31_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94244.1"
                     /translation="MIPDWKLERFLLGDLPELDMMQIRFEESQDDILRARINSLKESN
                     QEILSKYPFEKMNARFQTIVDKKKNRNLLFYAPMVAAAIILVAFIPFYAPEIASLWQS
                     PATVEIIEYAEDGTRIKGLSLNLEVWKKTSDSAEKLVEKSIVKAGDELQLRYSVPSKC
                     YGMIFSLDGNGKVTALLAEENSSVALEAGKIKLLPYAYKLDDAPHFEKFFLVVSNEAF
                     DLDVSQLDSLLNSKKVQVVTFIIQKEITSEGK"
     gene            9495..11111
                     /locus_tag="PLT31_03555"
     CDS             9495..11111
                     /locus_tag="PLT31_03555"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HPW94245.1"
                     /translation="MSVFFNVCIAVIILGFATFTNATPIERHVFAVSANNGGTDRPIL
                     RYAKTDAKSFVAVLSEMGGVQKNNAILLHEPSIKKLKQEFDQLDRRILESKKNGARQE
                     LLVYYSGHADDQGLHLGSEIYPWSDLRKRIDAMNASVKIAVIDACGSGAITRMKGGVA
                     VPAFMVDASSDMNGYAFITSSTQNEASQESDRLQGSFFTHSLVSGLRGAGDASGDGKV
                     TLSEAYQFAFNETLQKTQTTLGGAQHPSRDMNLTGTGDVVITDIRNTTAGLILDEKME
                     GRLFIRDDQNRLVAELQKISGRSLELGLPAGVYKVQMVIPEHNFVCDVKLKEGRKEKL
                     SNQKFSRVELSKTVSRGDVATDTTKKERRRRVTFNFIDIEDQPRIGSQFGVIMTSAED
                     EMAGSQFSLLLNKSRKEYSGVQLSVVGNLAKKMDGIQISTVYNIAQNLDGIQISSGLN
                     QLKEKSNGAQIGSINLTSDTLKGIQIGAINGANTVNSQIGSINWAKKSNLQISSINHA
                     SNVDIQIGSVNNAKNIGKVQVGVVNVAQKK"
     gene            11329..11685
                     /locus_tag="PLT31_03560"
     CDS             11329..11685
                     /locus_tag="PLT31_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94246.1"
                     /translation="MASGLGFGTQFGRYDTHFDLDFMTLLDYKNWSPSKIGSFDMDMD
                     EDFNLHYRVRLGATYKVFKGFGLMGGINANVVTEGSLSQVHIQPKGKYHAEWHIAGRD
                     VRVWPGLYAGLVFGKF"
BASE COUNT         3554 a         1940 c         2350 g         3935 t
ORIGIN      
        1 tattttgtaa agaatctccg tcagcgttaa gaactgttaa tgtggcaggg taaagaggcc
       61 cagaataata tccttcaatt tcatgactaa tgaaaagagt atcacctttt ttaaggtctg
      121 ataaccaaat gtattgctct tgtcttaaat aatatttggc ttgatcgttt cttggacgaa
      181 caacaacgag agtatcacca ggtcgaacga tcgagtctgg ctttgctaaa tattcgcctt
      241 tatccagagt tcttgaattg gtaattaatt caaaaaaggc atagggaccc gttaaataat
      301 ttgaaataga aagaggcttt aagagaacat taaaagtagt gcttttttga ggtaaaagga
      361 gtttgtctat ctgggcagtg tctttagcaa gagtgtctaa cccttcagtt aataaatact
      421 cattgatcca agagcctgag acgtcgatat tgagactaat tccagtattt gcctcaattg
      481 aaatatcaag tttacgtgga cccgatccaa tgataaagaa gcctcttaga gtgtcgtttg
      541 ttacaaaaga aacgctagaa gaatccccta tataagaata gtattcttcg tttatttcac
      601 tgaaaattct gagatgagaa ctttctttga tttccccttt tagttcgaga taataaaaac
      661 cctcttctaa ggtgataaat tgattggagt ctaattcact accagtacca gggagcatgt
      721 aattataaac attattaccc ttagcatcgg gtgttgggaa aattgtctta actaaagcgc
      781 cgtgttcttc atatatacga atggtatcgt tatttatacc tgtggtggct gcagcaattt
      841 taataagaga acctgctttt agatcgccca agtaaaaaag agcacgctca gaagtggtag
      901 aatcatcttc aaaataaaaa tttgctgttt cgtttgattc taaggtgtct acataagtca
      961 catcaagtcg attaattaat gaacttgaac ttgaagatgc ttctgaagaa gatgatgact
     1021 cttctggttt atttggttta tcgtcgccac atgcaatgaa aaaagtagct gttgttaaaa
     1081 gcagtaataa gaggtgtgta aaacgagtgt ttttcataat aagttttcct ctaattaatt
     1141 taatatataa aagctttgtt gggtggtgaa agcttcacca attatcttta aaatatactt
     1201 gcctgtaggg aatgaaatgg cttcgaattg gaaattgagt ttcttttcac tttccaaatc
     1261 tttttcggcg acaatcatca tgcgtttgcc aaattcatta tacacttcaa tttgaacaaa
     1321 ttggggataa ccaagaacta aatcaaagga acattttaag gtatagatat caatatttac
     1381 tttacttaat tgatatcctc cttgcattga ctcaccccca gctgattttg tgctatcaaa
     1441 ataactgata tacaaaacag gagcaaaatc tcgaccttgt tctttagctg aaatttggta
     1501 ttcagtggat gatttttctg tacccgttaa gatcaaggta tataaacccg aaggatgttt
     1561 ccgcttaaaa aattgggtga aactaggaga tctcaaaaag ctggaaaggg ttgctttggg
     1621 atcaatggtg ataacgccaa gatgatctgc aaagcgtgtg tcaacaccac caccaatgcc
     1681 ttttctcaca ttgaaaagac ctccaccttc gtctaaattc cctggagcat tggcccatgt
     1741 taattcagat tccttccatt caggaccaag tttgtcttct ccaaaattgt agtttttgtc
     1801 aatttccttt agtccaaaaa cattccattg aatgggtttc tttattttat caatgttttt
     1861 tacatttagt tgaagagcag catcagaaag aggccctggg agagcggata aatcaaattt
     1921 caaatatatt ttggagctat tatcattatt gtaactgatt cttaaaacag aatcattact
     1981 ttgaggtgtg tagttgtcaa attcggatac ccacgcatca gctcctctac cacttggcac
     2041 gttatctcca ttatggtcaa aagtactgta acgtttgtca aagacaaaga ctctcgctgt
     2101 atcggttcct tttaattgcc catcatgagc tttaaataaa agatcgtacc tgccgctcgc
     2161 tgtaaaagta acatctgttt cataactacg atgatctaaa aagactgctc gaccaggccc
     2221 tttcgctttt gtccattcca caggaacttc gcaaaggcca tcggcacgac cgtcatcatc
     2281 aacaattcct ttgagcctta ttttatttgg ctgtacaaga atttgttcgt ttaaggtttc
     2341 tgcttttggg gcaatattgt tacctgtttg aggaatataa cctacataaa gaggaatgtt
     2401 cactccacca gaagcacctc gctttaaatc tttagcataa agagttttcg ttaagccttt
     2461 tcttgtttct tcaataattt tatggaatgc gtttcctctg attgtagttt ctctaaggcc
     2521 ttcaattgaa atattagatg aatcattgat aaacatcggc tgtactttcg ctttgtcatt
     2581 agagatgaca tggacaccag ctaattctgc tttaccctta ttcttgttat gcaaaattgt
     2641 atttccattt tcagcagtga gacctagaac ccatatagta ccaccattat tgattatctt
     2701 ggtggtttta tcattgttca tgtgtagctg acgagcccac aagtgctgga agtttagttt
     2761 aatctgatct acagagacgt cttccatgta aacatctcca gccccatcta catcaccttc
     2821 ataacttttt accattacgt ttttcaaaag taataaacga ttacttttat gaaggatgcc
     2881 gttaccgaaa gaagaaaaac gttctattgt aagatcgggg aaggttccat caagaataat
     2941 aaagcggcca gttccttcaa ttcggccttc tgtaccaata atgtgacgta ctctttttct
     3001 aataaataca tctcgattga ttgtataacg cccttttgga ggaaaataaa ttgtctcagc
     3061 tccatcatca atggcttgtt gaatagcctt agatacgtct ctaccatcac ctgcagttcc
     3121 accgtaatca cctgtaattt ttacccagtt tgaaggagtt tgtttaggaa gttccatgac
     3181 ttctgctaca ggaactctca ttgatgcttt aggggagtga catagattaa tcagattttc
     3241 agaaacgtat tctaaaattt cttgaccctt aatcccttct cctaatccag gtgtagtgct
     3301 tttgattggt gttttgtatt tactggtggt tactccacgt acaaataaac cagcttcatt
     3361 aataatagct gggcctgcgg gctttttttt atttggaaca tattccagcg ttccatcaat
     3421 taaagtgagc atcgcatatt cgccagtatt gtaaatagcg ggtacgctat ttcggctacg
     3481 aaggccacga atggttaatg cttgatccca gttttctatc ccgtattttc tttggccaga
     3541 aagagaaatg tgctctagag tcattccaaa gaagggggtt cgggtataga taccgatatc
     3601 aaaaccacgg acttcaatat tttttaaaag taaaggccct acgttttcag caaaacccat
     3661 gtctattcca gcaactcctg caccgttttt atcgccagag aatattttca cattaaaaac
     3721 agttccctga ctagcggcat taaatcgaat accaatagca ccaggatttc cttttcctgt
     3781 tcgaagactc atgtcgcgaa cggcgttacg atgacgaggg tgaggcccaa tgcctgtgta
     3841 gatcatggct cttggaattt ctggattatc gaaaccacga gcgttgtctt taagaattaa
     3901 aatggttcca cccatacttt cgccttgtag gatggtgcgg cgataggctt ctgtaggttc
     3961 ttctgatttt ggccaactga gctgttttga aattttgtat gtcccatgag gcaagtagat
     4021 aatgaaatcc ccattcgggt gatcatctaa agctcgttga atggcttcgg tatcatcagc
     4081 ttttccatca ccttttgcaa aataaggctc ttttgtaaca tctacgactt cagaacccag
     4141 aggaaatgaa atttgagctg atattaggga gaataaagta aaaacaacaa aaagtatttt
     4201 ctgaaacacc ataacctcaa acaggaagaa tttctatgcc ctagtttaat attaatttag
     4261 aaaaaataag ccgatatttt tttaggatct ttgtagaagt gatctatatt caatgatgtt
     4321 gttttatttc gaaataaagc aagctcgaaa aataatgcta aatttaaaat cacatcgttt
     4381 tattttgctt ttttcttttc atacgcttgt tggagacaca tgcttttatt tcttgtctac
     4441 atcttcattt tgacttcttt tgcctttagt gaagatgtta cagagatttc gagttgtgaa
     4501 gaagggcaac ttgtatcaaa aattgagatg ttggaattaa agcatacaag aacccgtgtc
     4561 gtagagcgag aacttcttca tcaaataggg aaggcgtatt ctaagacgtt atggctaaaa
     4621 gaaaagggac gccttgaagg gttagattta ttcgccgaag tgcgattatc ttgtaaaaaa
     4681 gagtccagtg gattggtttt agaatatcat ttacctgaaa tattcaatat tatcccttct
     4741 cctgcaggta aaaagacgga ccaagacggg ttaatgttag ggctcgctct tgcccattta
     4801 aatttgggtg gagatgatat tcgagccgaa attcagtata gaacatcagt ttcgcctttc
     4861 ttttcttcaa atgaatatgc gttgtatgcg agttctcctt ggtttctaaa ccaaccgcta
     4921 ggttggaatt ttgagttttt acgcacttca agctgggata atcttcggct ttttgaagaa
     4981 gaatcatacc ttgcggacat agatcttcaa tggtttctta aaaaaccata tggattttta
     5041 ttttctctcg cttatcgcta tctaaataat tatggctaca cgccaagtgt ttctttaggc
     5101 tttttgattg actcacgcca gcccaatgta gactctcgtt ctggtatgta tcaagagtgg
     5161 ctttggactt ataatggggg tatagcgaat agtgatcaga attatcaaga atatttatgg
     5221 gattctcgtt tttatttcac tgtgaatcgt tttgtgtcag gagcttcttc tcttttaagg
     5281 gttaggctag gagaagttgc attttatgat cgttttcatc atggtggagc aaatactttt
     5341 cgaggttggg atcccgattc cctttgttat ggtacgcatg aatggatttt aaattttgaa
     5401 gaaagaattg ttttgaagga aagagcccct gtttcgattg gtggagtctc tcttttttac
     5461 ggattacaat gggttgttgg tgtcgatggg gctttcttat gggataaggg cttacctact
     5521 tggagtgatt accatagtgc attatacact gggcttcatg tcattattcc agccttagat
     5581 cgcattcgtt ttgaagttgg ttatagctct gatgaatctg agtttaagtt ctttattggt
     5641 ctttatgaaa aaaatattgc tcagagatgg agatcaagat aaaatgcgat ttttgggatt
     5701 tccctaccca aattcaaaat aaaaaatata ttatttatct gtaatactgt gattaaaaaa
     5761 ggaaatacta aaattagatg ctctaatctt agaggctttc caaagtgaaa aaaggcatgg
     5821 caataaatta tctggattta cctatcggcc caaaataccc ttacgaagta gattgtgtag
     5881 tagaaattgg aaaagacacc aatgtcaaat atgaatatga cgaacatttg catgtttttc
     5941 gcttggatcg ttgtcttttg tcttcaatga gttatccttg cagttatggt tttgtgccaa
     6001 gtacgttagc tgatgatgga gatgccattg atatgttgat ctataattcc gcttccttaa
     6061 atactggaac ggtggtcact tgccgtgtgg tgggcgtttt agatatgact gatggtggga
     6121 aaaaagacta taaagtatta ggagttcctc tttatactcc tcatcccatt cgagatattc
     6181 aagacgttga tccaatgttc ttaaagatca ctcgtaattt tttccaatac tacaaagagt
     6241 tagaagggaa aaatgttcaa ataggggagt ggttagacgc taagtttgct cgtgaaaaaa
     6301 ttgtgagtgc acatcattcc tattttcaga cacaagtgca aatgcctgag acgcgttacc
     6361 aggaaccaga gacggactcg agttgtgatt cgagtgttat tccttcgatt ttaatgtaat
     6421 gttataaaaa acgcaataat actttggtgt ctattattca aagaattttt gacagcaagt
     6481 ttattgcgat agacgtgatt taaaatggct cttaatcaaa gagcttttta ttatcatctt
     6541 ttaataaaaa gcgcacaaat aattgcgatt caagttttgc ttttgttggg ccatttgttc
     6601 caggcatact agatttaagt gctcttgaac cgcctccgag aaatccttca agaatcattg
     6661 attcaccgct gattgggctt tctgaaattg aaatcactga tgctattctt ttgttgctag
     6721 ttttagcgag agcttccgct ttgtcttttg ccttttcaaa agctttagcg gccattttgt
     6781 ttaatttatc ctctttctct ccaatttcat actgaacatt gactatttca atatcattta
     6841 tttgaattaa tccttctgat aataaatcag atttttcttc agaatggaac tgaattttaa
     6901 tggtttgggt caattggtaa ttcgaaaaag tttttttacc gtggtcccag ttccattgtt
     6961 ttctgagggt atagttttca aatgataaaa cgcttggatc aatcccaatt gaggagacta
     7021 aggaattcag ttcttttatt cgttcattct ttttggcgag aagctcttct ttttcttttc
     7081 caattaatga aatttgaagt tcccatagga tttcatcaga ggaaaacatc tctgaatggt
     7141 agccagaaac gtctatatat tgaatagctt gtttgccttc ttctgaaagg ggaatgcttt
     7201 tggggttttg tttgatgtat acaattgcag taattgtgat gcaaagagaa ataaaggcga
     7261 ttagaaattt catgttaggc ctcttttata agattattag gcttgtggtt tcatttataa
     7321 taatttaaat ataaatttgg ctttgataga attttaaatt aagggtgcct caaaattgtt
     7381 ttatgaaagt aatagacggt tttaattttt ctttttgtgg tgtcttttag gcttttcaat
     7441 acagttaaac aaaggataaa aatgaaaaag atcattctac tttttttatt agcagtccct
     7501 ttgactcatg ctgatataat tagaacgaaa gcttgtgatc gaggaaattg ctgtaattta
     7561 accattaacg atcgagatga tagctattct ttaaattgcg atcgtcgaaa tacagaagtc
     7621 attttatctg tatcagatcg tcgtggccgt cacgaattta atcaggtatt aagatctgat
     7681 cgtggtcgtt tagatcgtcg catttctcga caacattcgt ttagaatgga tattcgtgaa
     7741 gtcagagggc gtcatgaaac tgttgtagtg aaagaagggc ctcgtcgaca tgaaactatc
     7801 gttgttaaag acaattctcg tccaagaaga cgttctggtc cagcacgtat tcattatgaa
     7861 gaaccagatt gttatgatat tggttctggc cgtttccgga ttgaatatga atcaggtgag
     7921 gttttagtgg aagggcgttg ccatcgtcgt gatcgcgacg gtgaattctt attctataat
     7981 gattttggtg atttatggat gagagtgaat tatcacagag atcgtgctga gcgtatcgaa
     8041 tgtcgtgaca gacgtggtca ttggagaaac gttcgttccg aagacgattg tttccgcgat
     8101 tatgacgttc gtacgattcg tagagctaga agacgctaag caattttcgt tttttttggt
     8161 atttttgcct cagttttgat aactgaggta ttttttttaa ctcgtttagc aaatttccat
     8221 cgtttgggtg tttaataata gtgaatatga tgaaatcgtt aaaaaaagaa aaattcgagc
     8281 ttatttatca acaatatatg ccaatggttt tacgtcgttg tcgtttttta ttgaaaaatg
     8341 aagaagaagc ttttgatgtg gcgcaagatg tttttattcg gatactgtca aagcaagatt
     8401 ctttagatct tgaagctccc tctagtttat tatggaattc ggctactaga ttatgtttaa
     8461 atcgaattcg taataagaca agaagagatt tgaataggcc tacagaggat ttgctttgtc
     8521 aaattgcttg cgtccgagat gaaagagatg attatgaaca tcaatattta ctttctaagt
     8581 tatttcaaag tgagcctgct tcttctagaa caatagccgt tttacatttt attgatggaa
     8641 tgacttttga agaaactgcc cgaaatgtag ggctaacagt cggtcaagtt cgttatcgtc
     8701 tgaatacctt acaaaaaaag cttaaaagca tggaggaaaa atgatacccg attggaaatt
     8761 agaacgcttt ttgctaggcg atttacctga attagatatg atgcaaattc gttttgaaga
     8821 aagtcaagat gacattttaa gagctcgtat caattcatta aaagagagca atcaagaaat
     8881 tttatctaaa tacccgtttg aaaaaatgaa tgctcgtttt caaacgattg tagataagaa
     8941 aaagaatcgt aatcttttgt tttatgctcc aatggttgct gctgctatta ttttagtggc
     9001 gtttattcct ttttatgctc cagaaattgc ttctctttgg caaagtcctg cgaccgtaga
     9061 aattattgag tatgctgaag atgggacaag aattaaagga ctctctctta atttagaagt
     9121 gtggaaaaaa acatccgata gtgctgaaaa gcttgttgaa aaaagtattg ttaaggcagg
     9181 cgatgaatta caacttcgtt attctgtacc atcgaagtgt tatgggatga tttttagcct
     9241 tgatggcaat ggaaaggtga cggctcttct tgcagaagag aattcttctg ttgctcttga
     9301 agctggaaaa ataaaattgc ttccgtatgc ctataaattg gatgatgctc ctcattttga
     9361 aaagttcttt cttgtagtat ccaatgaggc ttttgattta gatgtttctc aattagactc
     9421 tttgttaaat agtaaaaaag tgcaagtagt gactttcatt atacaaaaag agattacttc
     9481 ggagggtaaa taggatgtct gtatttttta atgtttgtat cgcagttatc attttaggct
     9541 ttgctacttt tacaaatgca acacccattg aacgtcatgt atttgcggta agtgcaaata
     9601 atggtggaac agataggccc atattacgtt atgctaaaac agatgcaaaa tcatttgttg
     9661 ctgttctatc agaaatgggg ggtgttcaaa aaaataatgc catattatta catgaaccat
     9721 caattaaaaa gttaaaacaa gaatttgatc aattggatcg aagaattctc gaaagtaaaa
     9781 agaatggtgc aagacaagaa ttgttggttt attatagtgg gcatgccgat gatcaaggac
     9841 ttcatttagg gagtgaaatt tacccttggt ctgatctcag aaaaagaatc gatgccatga
     9901 atgcgagcgt aaaaatagct gtcattgatg cttgtggttc tggcgccatt actcgaatga
     9961 agggtggcgt tgctgtgcct gcatttatgg tagatgcatc aagtgatatg aatggttatg
    10021 cttttattac ttcaagtaca caaaatgaag caagtcaaga aagtgatcgt ttacagggat
    10081 ctttttttac tcattcttta gtgagtgggc ttcgtggagc aggcgatgcg agtggagatg
    10141 gaaaagtaac gctttcagag gcttatcaat ttgcatttaa tgaaacacta caaaaaactc
    10201 aaacgacgct tggtggggcg caacatccta gtcgagacat gaatttaacg ggaacaggag
    10261 atgtggtcat caccgatatc cgtaatacaa ctgctggact catcttggat gaaaaaatgg
    10321 aaggtcgttt gtttattcgt gacgatcaaa atcgacttgt ggctgaactt caaaagatca
    10381 gtggaagatc tttagaatta ggcttacctg caggtgtata taaagtacaa atggtgattc
    10441 ctgaacataa ttttgtttgt gatgtgaaac taaaagaagg gcgaaaagaa aaactttcca
    10501 atcaaaaatt ttctcgtgta gaactctcta aaacagtttc tcgtggtgat gtagctactg
    10561 atactacaaa aaaagaaaga cgtaggcgtg tgacttttaa ttttattgat atagaagatc
    10621 aacctcgtat aggaagccaa tttggggtta ttatgacgag tgcagaagat gaaatggctg
    10681 gttctcaatt tagcttgtta ttaaataaat caagaaaaga atattcaggc gtacaattaa
    10741 gtgtagttgg aaatttggct aaaaaaatgg atggaattca aatttcaacg gtttataata
    10801 tcgctcagaa tttagatgga attcaaatta gttcaggtct aaatcaatta aaagaaaaat
    10861 cgaatggagc tcaaattggt tcaataaact taacttctga tacattgaaa ggtattcaaa
    10921 ttggagctat taatggagct aataccgtaa atagccagat aggaagtatt aattgggcta
    10981 aaaaatctaa tctacaaatc tcatctatta accatgcaag taatgtagat attcaaatag
    11041 gaagcgtaaa taatgctaaa aacataggta aagtacaagt aggtgtagtt aatgtagctc
    11101 aaaaaaaatg atggccgttc tgtaggtgtt attaatatct gttctaattg taaaaaagct
    11161 cctattggac tgatcaatat tgttggtaat ggtgtatgga gtttttctgc aacagtagac
    11221 gaatctgcaa attttattgc aaacattcgt ttaggaacag cagcttttta taccatcttt
    11281 gagacttctc gttcatttaa ttataacgaa ggtgagtttg ataagggaat ggcttctggc
    11341 cttggttttg gaacgcagtt tggaaggtat gacactcatt ttgatttaga ttttatgact
    11401 ttattggatt ataaaaattg gtccccatca aaaataggat cctttgatat ggatatggat
    11461 gaggatttca atttacatta tagagttcgt ctcggggcta cctataaagt gtttaaaggc
    11521 tttggcctca tgggaggaat caatgcaaac gtggttacag agggctcttt aagtcaagtt
    11581 catattcagc ctaaagggaa atatcatgca gaatggcata tcgcaggaag agatgtccgt
    11641 gtttggcctg gcttatacgc gggcttagtc tttggtaaat tttaatttta atgcataaaa
    11701 aaaagccact tcaaagaagt ggtttttttt gtatttcttc aaaaccgaga gctaggctct
    11761 cggtccaaaa taagccttc
//
LOCUS       DAOOBB010000046        11715 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_7001_length_11715_cov_4.301372, whole genome shotgun sequence.
ACCESSION   DAOOBB010000046 DAOOBB010000000
VERSION     DAOOBB010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 11715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11715
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7001_length_11715_cov_4.301372"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1491)
                     /locus_tag="PLT31_03565"
     CDS             complement(<1..1491)
                     /locus_tag="PLT31_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94247.1"
                     /translation="MTTRTSFIKSAALGLTFVSTLAASPMYSPHKYQQNEWFGEFGGN
                     TSMYVNPAGIAETDQLEVSIGFFSTISGEASQEYVSLTYPIDYKHTLGFSFFENGATV
                     DGNHSYVENTLMLGYAYNLFQVLALGIDVSILQVDQFGSNQQFTIGADVGLSWNPIAN
                     SKFGFLQIGLALQNVLQPAISTAEESSGYKFILMTEEEAYKIPMNLNFSVFYRGLNRM
                     LEAKAEVSVIDLVHDEEEGGKGQNIETSFTLTYYLSPFLGVRVRFTKEGYPVAGATVN
                     VKDLKIFRYLALDLEMSHDDIYEQKNRGFIWAVKLTSRFGNTREEKIGEERYRRLKIE
                     PENDYRAAMRLYLNRQFLEAAYAFGKVQTKYPAFHLVDQAAFYKAKSFENLRMHKAAK
                     SIYEDAIKRYPQSDQRAKYHFQLMNIDYKEGKYSEAIVKYQGIAQRFGESDVKADADY
                     IAGQIKFEQGLYQEAVDLLASILPGNANYFYARYTMGIAYSRMSKWD"
     gene            complement(1506..2945)
                     /locus_tag="PLT31_03570"
     CDS             complement(1506..2945)
                     /locus_tag="PLT31_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPW94248.1"
                     /translation="MPGDYPKIADALGNADAGDTILVRRGVYNENITLIMGVVLKGED
                     PVTTIIDGGRRGPTVMGTSGAEISHFTIRNGIEGVLCENAAPYIHHCYIIDNKATGIG
                     AFISLPHVRNNVVYGNRWSGILAWGAKSLDAYIEQNVVLRNGYSGLALKGPSNIIARN
                     NIFMENHYYGVFADPASGQTKIEYNNIYKNYYPFNRFIKVNRTNVSLDPAFINPSLSK
                     PNFYCAPSSPMLKRGKGKLDIGLTAIEAIQKEEEVEETRSPDTDADGLCDAWVSEEGA
                     SEKYAAVCTGLDNCAEEAEDFDGYQDDDGCPDADNDRDGLCDPWVEAKGLLNQFAHVC
                     KKVDLCPEQAESMNGYKDEDGCPDEVPVPPKKVFILEGVNFESGKATITQDSYVSLMK
                     VVDIMEAFPETTFEIVGHTDNVGRPEKNKQLSADRAESVKNFLVEKGVSESRMVTRGV
                     GDSEPIAANTTAEGRAKNRRIEFTRTDIK"
     gene            complement(3236..3424)
                     /locus_tag="PLT31_03575"
     CDS             complement(3236..3424)
                     /locus_tag="PLT31_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94249.1"
                     /translation="MNLVQTLEQQLEAFKREYEKFERGNKSAGTRARKALQDIKRTCQ
                     DLRVSIQGSKKEDAGSEE"
     gene            complement(3583..4383)
                     /locus_tag="PLT31_03580"
     CDS             complement(3583..4383)
                     /locus_tag="PLT31_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HPW94250.1"
                     /translation="MIPLKLGISTCPNDTFIYEHLVAGISHSPFQWQVIYADVQTLNE
                     MVLHGELDVAKVSCGVLPLIENNYRVLSSGGAIGYGCGPLLLSSEAREFNPSQMTQLP
                     GENTTAALLFRYWFENEYNGSSLLTSYDFFDQVYKNLCLKRISQGVVIHEHRFTWKRD
                     GLRLIQDLGAYFETKTGAPIPLGCTVARQLFSEDLIVSIEKEIQKSIDDAWNRNFLVT
                     PFIKEKAQIEDDSVIEDHIRMFVTDFSYNMGKKGFDALKELFSIVKKN"
     gene            complement(4380..5039)
                     /locus_tag="PLT31_03585"
     CDS             complement(4380..5039)
                     /locus_tag="PLT31_03585"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HPW94251.1"
                     /translation="MFRQLIAFASSLECQKVFPEIDTKSFKSLFFHLTDDVDVAVLDV
                     GILPFTVALTSLFSKNYWPVVIQVGIAGAYMNRGLCIGDVVRVDTEILGDQGFEKVDG
                     SFQSWPLKENQEPISFSASDLSFSPPEIRMLKGVRGLTVNTCTGTSKTAENRCRSFNV
                     DVESMEGASFLALAPLLNFKAYEVRAISNYVSDRNKSDWRIDDALAHLRTKIINPMVF
                     S"
     gene            complement(5039..5998)
                     /locus_tag="PLT31_03590"
     CDS             complement(5039..5998)
                     /locus_tag="PLT31_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPW94252.1"
                     /translation="MLNNTLILGLNPAWQRLFITEKFELGEVHRLPKAVEFGSGKGIN
                     CGRVLQLLGGNPVMMHFLGGSHGSCIFDELSNAGIQQVPIWIQTPTRICTTVVSDGVT
                     TELIEPSPVLSDLENEDFIQTLSENWEDVNRIVVCGSFPINFESETLLNIDLKDKRLF
                     VDAINDIDKWLEKGVELLKLNANEYFKLLQHMSIPQVTSSPQFWKITASTVLERLPIH
                     YLVVTDEDGPVRVFFMVEGKFQFIQLQPPSIKVVNSIGAGDSFLAGWLRADALDFSLE
                     ETICRATAVAVARCEVERPWNLKLDRVHQLEKELLSAIERIAD"
     gene            complement(5998..7302)
                     /gene="tyrS"
                     /locus_tag="PLT31_03595"
     CDS             complement(5998..7302)
                     /gene="tyrS"
                     /locus_tag="PLT31_03595"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPW94253.1"
                     /translation="MPFRPVKEQLDILLRGVIEIVPQEELEKKLQKSYETGIPLRIKL
                     GVDPTAPDVHFGHTVVMRKLRQFQDLGHTVVLIVGDYTAMIGDPSGRNKMRPRLSHEQ
                     VLENAKEYQEQFFKVVRRDQVEIRYNGEWFSKLPFNKVTELMGQFTVAQMLEREDFNN
                     RYTSNQPISLHEFMYPMMQGYDSVAIASDVELGGTDQKFNVLRGRDLQLVEGMEPQIG
                     MFMPILLGTDGKVKMSKSIGNYVGLNEPADVMYHKIYSLADNLIANWFELLTTVPLDE
                     IHLMVEKIAKGEMNPNEAKHLLAINIVSQYYGEAEAEAAAKKEREVHSGNAIPSDAIE
                     CKLNPGSYGLLDLLAEIKAFPSKGEARRMIQNGGVRIRFKVESDFNINDKVIRNDPPA
                     NIIQLDNQWFEWKVDDPQSFVKFEKSSFVVQIGKRKFFKVNF"
     gene            complement(7514..7966)
                     /locus_tag="PLT31_03600"
     CDS             complement(7514..7966)
                     /locus_tag="PLT31_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HPW94254.1"
                     /translation="MSSTLLQKILDDIKVAMKSRDALVLEVLRTLHSDIKNISINSGK
                     EITDEIVIDVLAKSVKQKNEAIEMLKAGGREEKVASEEAAIEIYKRYLPQELSEEEVI
                     ALIAQIKESTGAQGPKDMGKVMKEITPQVKGRFDAKRVSVLVQEALKS"
     gene            complement(8034..8243)
                     /gene="rpsU"
                     /locus_tag="PLT31_03605"
     CDS             complement(8034..8243)
                     /gene="rpsU"
                     /locus_tag="PLT31_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HPW94255.1"
                     /translation="MIGVIVKSNEPFERALKRFTKSCEKNGIISDVKKRQRFEKPSEV
                     KKRLNTAARRKRLKEIADLNRKRLY"
     gene            8443..9252
                     /locus_tag="PLT31_03610"
     CDS             8443..9252
                     /locus_tag="PLT31_03610"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPW94256.1"
                     /translation="MKKSFFIVLATSVLASQLFLACSKEEEVVPEIDLQKTKNAATSE
                     KQDSEEPVLQPIQALTTTSKAEEATPAQTTKPTSFASTNAIEQGSSGPYVIQVSIQPS
                     KSNANSIVEKLSSNNIDAYIANVEDPGELEGTYYRVRIGYFTSIQDAQNFGKQTLEPL
                     GYAWWVDNKSNDNVGNSESDYEEYEDAEEEEYEEEEVVEAPAPAPTPAPAPAPAPTPA
                     PAPAPAPTPAPASEPAAPVAQPAPQVAPAAPAAPAPQTPAPETEEVIDDWE"
     gene            9278..10588
                     /gene="rimO"
                     /locus_tag="PLT31_03615"
     CDS             9278..10588
                     /gene="rimO"
                     /locus_tag="PLT31_03615"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HPW94257.1"
                     /translation="MPIKKNSVFTIHLGCSKNQVDAECMLSELLASDFSISQESSTAN
                     YIIINTCGFIEPAKEESIDIILSQIKDKKKSQKIIVTGCLSERYKHDLPIEIPGVDYW
                     LGTYKPGELLKLLGFSEKHICETVPTNRINFGSFSHHAYLKIAEGCNRRCAFCAIPNI
                     RGKQVSRSIDELVLEAQQLEAQGVKELTLVAQDTTYFGREKGKKGGTLQTLLEALLEH
                     TTIPWIRTLYWYPDFIDDGLLDLIAKEPRLCKYIDMPIQHASDRQLKLMSRRYNQSTL
                     YSLLNKIKEKIPGVSLRTTVLVGFPGESHEDFEELMSLIQKIQFDHLGGFIYSPEEDT
                     PAEKITADTVSESEARLRLDMITEYQEDLAMERNEALIGKKIRIIIDEVAEESEFHFY
                     GRTEGSALDTDDIVKVIEGNAEIGSFYDALVVDAAPHELICNIL"
     gene            complement(10708..10781)
                     /locus_tag="PLT31_03620"
     tRNA            complement(10708..10781)
                     /locus_tag="PLT31_03620"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10745..10747),aa:Arg,seq:ccg)
     gene            complement(10855..>11715)
                     /locus_tag="PLT31_03625"
     CDS             complement(10855..>11715)
                     /locus_tag="PLT31_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94258.1"
                     /translation="SFAVWAQNDEDMDSSEDVDTEEVSEEETEEEDVEEDAPSVAAGS
                     NYNEHLSRKKFEEDQRADEFANALRRRDWLKDRLILQVGMGSRMPVMGETGIGMGFGA
                     GVEYITRWHLAAYASIGMVPSGIDNYFEDINLQDGMGWKFGLNYYLFAKNAIHLGFSA
                     SYGTVHFDHDVVADENNNMLRSIIMLEGYQFDILVTYLTSEWYYLQFAFGFYYAPTAQ
                     NDDVSFRTKSTTSFELVSRVVNPDGIPEYGLVFGITLGFGFPEFFPDDTEKRRRAREK
                     ERSNAQQKAW"
BASE COUNT         3764 a         2415 c         1970 g         3566 t
ORIGIN      
        1 gtcccactta ctcatacggc tataagcaat acccatagta taacgagcat agaagtagtt
       61 cgcattacca ggaagaatag aagctaaaag atcaactgct tcttgatata agccttgttc
      121 aaacttaatc tgaccagcaa tatagtcggc atctgctttc acatcacttt caccaaaacg
      181 ctgtgcaata ccttggtact taacaatagc ttcgctatac tttccttcct tatagtcaat
      241 attcattaac tggaagtgat atttggcgcg ttggtcagac tgaggataac gcttaatagc
      301 atcttcataa atggattttg ctgctttatg catacgcaaa ttctcgaaag attttgcttt
      361 atagaaagcg gcttggtcca ctaaatggaa agctggatat tttgtttgaa cctttccaaa
      421 agcatatgct gcttcgagga actgacgatt caagtacaaa cgcatagctg cacggtaatc
      481 gttttcaggt tcgattttca aacggcgata acgttcttca cctattttct cttcacgagt
      541 atttccaaag cgagaagtta atttaacagc ccatataaag ccacgattct tttgttcata
      601 aatatcatca tgagacattt cgagatctaa agctaaatat ctaaagattt tcaagtcttt
      661 gacgttcaca gtggcacctg caacaggata gccttctttt gtgaagcgga cacgaacacc
      721 taagaatgga cttaagtagt atgttaaagt aaagctggtc tcaatatttt gaccttttcc
      781 accttcctct tcatcgtgaa cgagatctat aacagaaact tctgctttag cttcaagcat
      841 gcgattcaaa ccacgataaa acaccgagaa gtttaaattc attggtattt tataagcttc
      901 ttcttctgtc atcaagataa acttataacc acttgattct tcagcagtac taattgcagg
      961 ttgaagtacg ttttgaagag ctaatccaat ttgtaagaag ccgaatttgg agttagcaat
     1021 tggattccag ctaagaccta catccgcacc tatagtaaat tgttgattac ttccaaattg
     1081 gtccacttga agaatagaaa catctatacc aagagcaagt acctgaaata ggttatatgc
     1141 ataaccaagc atgagtgtgt tttcgacgta agaatggttt ccgtcaactg tagcaccatt
     1201 ttcaaaaaat gaaaagccta atgtgtgctt atagtcgatt gggtatgtca aggaaacata
     1261 ttcctgactt gcttctccac tgatagtgct gaagaaacca atacttactt ccaattgatc
     1321 cgtctctgca atgccagctg gattcacata cattgaagta tttccgccga attctccaaa
     1381 ccattcattt tgttggtatt tatgtggaga atacattgga gaggcagcta gagtgctgac
     1441 gaaagtgagc ccgagggctg ctgatttgat gaaactagta cgagtcgtca cctaaccttc
     1501 tccttttact tgatgtccgt gcgagtaaat tcgattctac gattcttcgc acgtccttcg
     1561 gcagtcgtgt ttgcagcgat aggttctgag tcacctacac cgcgggttac cattcggctt
     1621 tctgaaacgc ctttttccac aaggaaattc ttgacggatt cagcgcgatc tgcagaaagc
     1681 tgtttattct tttccggacg gccgacatta tcggtatgcc ctacaatttc aaacgttgtc
     1741 tcagggaacg cttccataat atcaaccact ttcattaatg acacataaga atcttgtgta
     1801 atagtggctt tacctgattc aaaattcaca ccttccaaaa taaatacttt cttaggaggt
     1861 acaggaactt catctgggca accatcttca tctttataac cattcatgct ttcagcttgt
     1921 tctgggcaca agtccacttt tttacaaaca tgagcaaatt ggttcaaaag acctttagct
     1981 tctacccaag gatcacaaag accatcacga tcattatcag catcaggaca accatcgtca
     2041 tcttgataac catcaaaatc ttcagcttct tcagcgcagt tatctaaacc agtacaaaca
     2101 gcagcgtatt tttcagaagc accttcttct gaaacccacg catcacaaag accatctgcg
     2161 tcagtatcag ggctacgtgt ttcttcaact tcttcttctt tttgaatggc ttcaattgct
     2221 gttaaaccaa tatccaattt accttttcca cgcttgagca ttggagaaga tggagcacaa
     2281 tagaaatttg gtttacttag agaaggatta ataaaagctg gatctaagga aacgttagta
     2341 cgattcactt tgataaaacg attaaatgga taatagtttt tgtaaatatt gttgtactca
     2401 atctttgttt gaccagatgc agggtcagcg aaaacaccat aataatggtt ttccataaag
     2461 atattattac gagcaataat attcgaagga ccctttagag caagtccaga atatccattt
     2521 cgtaaaacaa cgttttgttc aatgtaagca tctagtgact tagcacccca tgcaaggata
     2581 ccagaccagc gattaccata cactacgtta ttgcgaacat gtggtagcga aatgaaggcg
     2641 ccaataccag ttgctttgtt gtcaatgatg taacaatgat gaatgtaagg agcagcgttt
     2701 tcgcaaagga caccttcaat accattgcga atcgtgaagt gcgatatttc agcaccagag
     2761 gtacccataa ctgtaggacc tcgacgacca ccatcgataa ttgttgttac aggatcctca
     2821 cctttaagca caacgcccat gatgagggtg atattttcat tatatacgcc acgtcgaacg
     2881 agaattgtat ctcctgcgtc agcatttccg agcgcgtctg ctatcttcgg gtaatcgccg
     2941 ggcacattta taacctttgc cgcggcagtt cctgaaagaa gcattgcccc ggctagaata
     3001 aagaccagtt tctttagggt catattgcta ggtttctcca atcataaaac acgtataagt
     3061 ttacaatcaa caccgatggt ttgtcaaaga atcgctttaa tcaaacctaa gtccaagtgg
     3121 gaatatacct tcaaaaacat cgttagtcaa atactttctt attcattttt aagattatta
     3181 aaaaaaacaa gaaacatttg actaagctag tttaatcaaa ttcccaattg aacgcctatt
     3241 cttcagagcc ggcgtcctcc ttcttagagc cttgtataga cacacgcaaa tcttgacagg
     3301 ttcttttgat atcctgcaaa gccttacggg ctctggttcc agcggattta tttcctcttt
     3361 caaacttttc atattcacgc ttgaatgctt caagttgttg ttcgagtgtc tgtactaaat
     3421 tcataacgat actcccaaaa aaaggtgttg tttggaaaat aaataaagtt ttaatcaaaa
     3481 atcacgttta ctgtaaaatt atttttacgt taatcatttt attacaaaag atttcaaaag
     3541 aaattcattt tatagaaaaa aaataaaaaa aaaagatcat ttttaatttt ttttaactat
     3601 agaaaaaagc tcttttagag catcaaaacc cttttttccc atgttatatg aaaaatcagt
     3661 cacaaacatt cgaatatgat cttctataac agaatcgtcc tcaatctgag ctttttcttt
     3721 aataaaaggg gtgactaaaa agttcctatt ccatgcatca tcaatacttt tttgaatttc
     3781 tttttctatc gaaacaatta aatcttctga aaagagctga cgtgccacgg tacaacctaa
     3841 aggaattggt gcccctgttt ttgtttcaaa ataggctcct aaatcctgta taagccttaa
     3901 accatccctt ttccaagtaa aacggtgttc atgaataacg actccttgag agattcgctt
     3961 gagacataaa ttcttataga cttgatcaaa aaaatcatac gacgttaaaa gagaagaacc
     4021 attatattca ttttcaaacc aataacgaaa taataaagcg gccgtagtat tttcacctgg
     4081 tagttgcgtc atttgactag gattaaattc ccttgcctct gatgaaagaa gcaagggacc
     4141 gcaaccataa ccaatggcac caccagaaga aagcacccta taattgtttt ctattaaggg
     4201 taaaacgcca caacttactt tggctacatc tagttctcca tgcagaacca tttcatttaa
     4261 ggtttgtaca tcagcataaa tcacttgcca ctggaatgga gaatgagata tgcctgcaac
     4321 caaatgttcg taaataaacg tgtcatttgg acaagtagaa ataccgagtt ttaagggaat
     4381 caagagaata ccattggatt aattattttt gttcttaaat gagctaaagc gtcatctatt
     4441 cgccagtcag atttatttcg atcagagacg taattactta tagcacgaac ttcataagct
     4501 ttgaaattta atagaggagc tagagctaaa aaagaagcgc cttccatgga ttcaacatct
     4561 acgttaaaag aacggcaacg attttctgct gttttagaag tccctgtaca agtatttaca
     4621 gtaagccctc gaactccttt taacatacga atttctggcg gcgaaaagct taaatcactt
     4681 gctgaaaagg aaataggctc ttgattttct tttaatggcc atgattgaaa agaaccatcc
     4741 actttttcaa aaccttgatc gcctaagatt tcagtatcta cacgaacgac atctcctata
     4801 caaagacctc gattcatata agctcccgca atccccactt gtatcacgac aggccagtaa
     4861 tttttagaga aaagagatgt taatgcaact gtaaaaggca aaataccaac atctaaaacg
     4921 gcaacgtcca catcatctgt caaatgaaaa aaaagacttt tgaaagattt cgtatcaatc
     4981 tcaggaaaca ccttttgaca ctcaagagaa gaggcaaagg ctattaactg acgaaacatt
     5041 aatccgcaat cctttctata gcagagagta gttctttttc cagttgatga acacgatcta
     5101 attttagatt ccatggacgt tctacttcac aacgagcgac tgcaactgct gtagctctac
     5161 aaatggtttc ttcaagagaa aaatcaagcg catctgcacg aagccagccc gctaaaaagg
     5221 aatctcctgc accgatgcta ttcaccactt ttatagaagg tggttgtaat tgtataaact
     5281 gaaactttcc ttctaccata aaaaagacac gtacaggacc atcttcatct gtaacgacta
     5341 ggtaatgaat aggtaagcgt tcaagaactg tggaagctgt tattttccag aattgcggac
     5401 tggaagtaac ttgagggata ctcatgtgtt gcaatagctt aaaatactca tttgcattta
     5461 gttttaagag ttctacccct ttttctagcc acttatctat gtcgttaatg gcatccacaa
     5521 aaagtctttt atctttcaaa tcaatattaa gaagcgtttc agattcgaaa ttaataggaa
     5581 atgatccaca tacaacaatt cgatttacat cttcccaatt ttcagaaagc gtttgaataa
     5641 aatcttcatt ttctaaatcg gaaagtacag gagaaggttc aattagttct gtagtaacgc
     5701 catcactcac cactgtggta caaatacgtg ttggcgtctg tatccaaatt ggaacttgtt
     5761 gaatccctgc atttgaaagt tcatcaaaaa tacaagagcc atgacttcca cctaaaaaat
     5821 gcatcatcac gggattgcca ccaagcagtt gtaatactcg gccacaatta ataccctttc
     5881 cagatccaaa ttctactgcc ttaggaagac gatgaacctc cccaagttca aacttctcag
     5941 tgatgaataa tcgctgccaa gcaggattta aacctaaaat cagggtgtta tttaacatta
     6001 gaagtttacc ttgaaaaact tacgtttacc tatttgaact acaaaagaag atttttcaaa
     6061 tttcacaaaa gactgtggat catcgacttt ccattcgaac cattgattat ctaactgaat
     6121 gatattcgca ggtggatcat tacgaataac tttatcattg atattaaaat cggattccac
     6181 tttaaaacga attcgaactc caccgttctg aatcatacga cgagcctctc ccttgctagg
     6241 aaaagctttt atttcagcta acaaatctaa caagccataa gaacctggat tcaacttgca
     6301 ttctatggca tcgcttggaa ttgcattacc actatgcact tctctttctt tttttgctgc
     6361 ggcctctgct tcagcttctc cgtagtactg agaaacaata ttaatcgcca atagatgttt
     6421 tgcttcattg ggattcattt cccctttcgc tattttttca accataaggt gaatttcatc
     6481 tagtggcaca gtagttaaaa gttcaaacca gtttgcaatt aaattgtcag ctaaagaata
     6541 aattttatgg tacatgacat cggctggttc atttaagcca acgtaattgc ctatggattt
     6601 actcattttt actttgccat ctgtccctaa aagaataggc ataaacatac ctatctgagg
     6661 ttccataccc tctactaatt gaaggtctcg gccacgaaga acattaaatt tctgatctgt
     6721 tccacctaat tctacatcgg aagcaatggc aacagaatcg tagccctgca tcataggata
     6781 catgaattca tgaagagaaa taggctggtt gctcgtatat cgattattaa aatcttctcg
     6841 ttcaagcatt tgtgctacag tgaactgccc cattaattca gtcactttat taaaaggtaa
     6901 tttggaaaac cattctccat tatagcgaat ttctacttga tctcgacgaa cgaccttaaa
     6961 aaattgttct tggtattctt ttgcattttc taaaacctgt tcatgagaaa gtcgagggcg
     7021 cattttgtta cgaccacttg gatcaccaat catcgctgtg taatcaccta cgattagcac
     7081 aacggtatga cctaaatctt gaaactgacg taactttctc atcacgacgg tatgaccaaa
     7141 atgaacatca ggggctgttg gatctacacc taatttgata cgaagaggaa ttccagtttc
     7201 ataagatttt tgaagtttct tttcaagttc ctcctgtggt actatttcaa taacaccacg
     7261 aagtagaata tccaactgtt ctttcactgg tcgaaatggc atagacaaaa tcctttatct
     7321 tacgggttct tctaaacagc ctaatcctct tttaaagaag cccttatctc gttcattaat
     7381 ttagtaaaaa ttaaaaacaa agattaaaag agacatttag atgacaatat ttttaattaa
     7441 gccttatttt tttgaaaaaa aaagaacttc attttttgaa gttctaaaaa tctgaaaaaa
     7501 ttacaagtat ttactatgac tttaatgctt cttgaactaa aacgctcact cgctttgcat
     7561 caaaacggcc cttgacttga ggagtaattt ctttcattac tttccccata tccttaggac
     7621 cttgagcacc agtggactct ttaatttgag caatcaaggc aatgacctct tcttcggaaa
     7681 gttcttgagg gagatagcgt ttataaattt caatagctgc ttcttctgat gcaacttttt
     7741 cctctcgccc accagccttt aacatttcaa tagcttcatt cttctgcttt acacttttag
     7801 caagaacatc aatcacaatt tcatctgtga tttctttacc agaatttatt gaaatattct
     7861 tgatatcaga atgtaatgtt ctcaacactt caagaactaa agcatctcta cttttcatag
     7921 ctactttaat atcatcaaga attttttgta ataaagttga actcatttta actatcctga
     7981 taaaaagaaa aacaggaagt aaagatactt cccataaaag tcaaaaaaga atcttagtac
     8041 aaacgcttgc gatttaaatc agcaatttct ttaagacgct tgcgacgagc cgctgtatta
     8101 aggcgtttct tcacttcaga tggtttttca aaacgttgac gttttttcac atcggaaatg
     8161 atgccgtttt tttcacaaga cttggtaaag cgcttaagag ctctttcgaa gggttcgtta
     8221 gacttaacaa ttacgccgat cacgatgttc ctttggttac aaatttaaga cagccaaata
     8281 tacgtaaatg cttttttttc gtcaaccatt catctatcat tcttcaaaaa gatgcttttt
     8341 attcgtaata acgttatttt ttttctattc taaatgatta taacttgctg ataataaaag
     8401 acttatatat atttttacaa tgaccttttt aggagagctc ctgtgaaaaa atcttttttt
     8461 atcgtgcttg cgactagtgt tctagcctct caactctttt tagcttgttc taaagaagaa
     8521 gaagttgtac cagaaataga tttgcagaaa acgaaaaatg cagctacttc tgaaaagcag
     8581 gactccgaag agcctgtatt acaaccaatt caagctctta caactacttc aaaagccgaa
     8641 gaagctactc ctgcacaaac cacaaagccc acttcttttg catccacaaa tgctattgaa
     8701 caaggctcaa gtggcccgta tgtaattcaa gtgagcattc aaccatcaaa aagcaatgcc
     8761 aattccattg ttgaaaagtt aagttctaac aatattgatg cttatatagc caatgtcgaa
     8821 gacccaggtg aactcgaagg aacttattac agagtacgaa tcggttactt cacttcaatt
     8881 caagatgctc aaaactttgg taaacaaact cttgaacctc tcggctatgc ttggtgggtt
     8941 gataataaaa gtaatgataa tgtaggaaac tctgaatctg attacgaaga atatgaagac
     9001 gctgaagaag aagagtatga agaagaagaa gtcgttgaag ctccagcacc agctcctaca
     9061 ccagcaccag ctcctgcacc agcgccaaca ccagctcctg caccagctcc tgccccaaca
     9121 ccagctcctg cttctgaacc agcagcccca gtagcccaac cagcacctca agtagcacca
     9181 gccgctcctg cagcccctgc accacagact cctgctccag aaacagaaga agttattgac
     9241 gactgggaat aagcattgac ttttttatat ttgagtcatg cccattaaaa aaaattctgt
     9301 tttcaccatt cacttgggat gttcaaaaaa ccaagtggat gctgaatgta tgctttctga
     9361 actactagca agtgatttct caatttctca agaaagctct actgcaaatt acatcatcat
     9421 caatacttgt ggctttattg aacctgcaaa agaagaatcg attgacatta ttttgtctca
     9481 aatcaaagac aagaaaaaat ctcaaaagat catcgttaca gggtgtttaa gtgaacgata
     9541 caagcatgat cttccaatag aaattcctgg agtagattac tggcttggta cttataagcc
     9601 tggagaatta ttaaagctat taggcttttc tgaaaaacat atttgtgaaa cggttccaac
     9661 aaacagaatt aattttgggt ctttttctca ccatgcctat ttgaaaatcg ccgaaggctg
     9721 taatcgtcgc tgtgcttttt gtgcaatacc caatattaga gggaaacaag tttctagaag
     9781 catagatgaa cttgttcttg aagcccaaca gcttgaagct caaggggtaa aggaattaac
     9841 tcttgtagct caagacacta cctattttgg tagagaaaaa ggaaaaaaag gtggcacgct
     9901 tcaaactctt ttagaagctc ttcttgaaca tacgactatt ccttggatta gaactcttta
     9961 ttggtatcca gactttattg atgatggatt gcttgatctt attgcaaagg aacctaggct
    10021 ctgtaaatac attgacatgc ctattcaaca tgcaagtgat cggcaactga aattaatgtc
    10081 gagacgttat aatcaaagta ctctctacag tctactaaat aaaattaaag aaaaaatccc
    10141 aggtgtttct ctaagaacga ctgttcttgt tggcttccct ggagagtctc acgaagattt
    10201 tgaagaatta atgtctttga ttcaaaaaat acagtttgat catcttggtg gttttattta
    10261 tagcccagaa gaagatactc ctgctgaaaa aattacagct gatactgtta gcgaaagtga
    10321 agcccgctta aggcttgata tgattactga ataccaagaa gatttagcta tggaaagaaa
    10381 tgaagctctt attggtaaaa agattagaat catcattgat gaagtagcgg aagaaagtga
    10441 atttcatttt tatggaagaa cggaagggag tgctttagat acggatgata tcgttaaagt
    10501 gattgaagga aatgctgaaa ttggaagttt ttatgatgct ctggttgtag atgctgctcc
    10561 ccatgaactc atatgcaata ttctttgatt catctctaat acagctttac aaaatctttc
    10621 aataacgatt aaataaatca aatataacat ttcttgtaat ttcaaaattt atgcaaaaag
    10681 ggtgccttat aaaagcaccc tttttaatac acctgatgcg attcgaacgc atgaccttca
    10741 acttcggagg ctgacactct atccaactga gctacaggtg caaagttttc caataataca
    10801 tttttttgtg tataaaaaaa gcccgctttt aacgggctta agacttttta taaattacca
    10861 agctttctgt tgagcatttg acctttcttt ttcacgtgca cgacgtcgtt tttctgtatc
    10921 atctgggaag aattcaggga aaccgaaacc aagagtaata ccaaatacta acccatattc
    10981 tggtatacca tctggattaa caacgcggct tacgagttca aaactagttg tactttttgt
    11041 acggaaagaa acatcgtcat tttgtgccgt tggagcatag taaaagccaa aagcaaattg
    11101 caaataatac cattcagaag taagataagt caccaaaata tcaaactgat atccttcaag
    11161 cataatgata ctacgtagca tattattatt ttcatcggca acgacatcat gatcaaaatg
    11221 aactgttcca taagaagcag aaaagcctaa atgaattgca ttttttgcga acaaataata
    11281 gtttaagcca aacttccagc ccatgccatc ttgaagatta atatcttcaa aataattatc
    11341 aataccgctt ggtaccatgc ctatagaagc ataagcagcc aaatgccagc gtgttatata
    11401 ttcaacacca gcaccaaaac ccataccaat accagtttca cccatcactg gcatacgaga
    11461 acccataccg acttgtaaaa tcaaacgatc tttgagccag tcacgacggc gtaatgcatt
    11521 agcaaattca tctgctcgtt gatcttcttc aaatttttta cgagaaaggt gctcattata
    11581 attagaacca gcagccacag aaggagcgtc ctcttcaacg tcctcttctt cagtttcttc
    11641 ttcgcttact tcttcagtat caacatcttc tgaagaatcc atatcctcat cattctgagc
    11701 ccatacagca aacga
//
LOCUS       DAOOBB010000047        11672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_7038_length_11672_cov_4.477490, whole genome shotgun sequence.
ACCESSION   DAOOBB010000047 DAOOBB010000000
VERSION     DAOOBB010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 11672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11672
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7038_length_11672_cov_4.477490"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="PLT31_03630"
     CDS             <1..534
                     /locus_tag="PLT31_03630"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HPW94259.1"
                     /translation="SDVNIDSIKKQLLTLPLVHSAVVSTSFPSTLEIVLEEATPLMVS
                     FDKNKWKVFSERAKVLPTAILSAYQLPVVDLMDEEEMKQIGTFLSAMKAKDEFLYRKV
                     SQIGVNKKEYAIEVFFKDVEFKTLFPLSGWEEKQFDQYKQLIQGFTSDLKTASIVDMR
                     FNGFAYIKPYEKRYSDG"
     gene            527..1786
                     /gene="ftsA"
                     /locus_tag="PLT31_03635"
     CDS             527..1786
                     /gene="ftsA"
                     /locus_tag="PLT31_03635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HPW94260.1"
                     /translation="MDDKNKMTVNKENIIVGLDIGGSKIAVFIGLQENQNQVRVLGFG
                     VKELKKNPISDLDFLISSIGSAVKEAETMTGFDVKEVYVGVAGDYISCFKSKGGVTLD
                     PSKNEVKESHVAAVIKHASTLPSTAGEILHIFPRNYSLDDVQGIKNPIGMSGVRLDAE
                     VQIVAAKTLNLQNHKKSVEKAGFEIKAMVLEPLAAAYAILHDEERELGVAIIDIGATS
                     ADLAVFYEDAVCYSVSLDLAGNIITSDIAKSFDLPISIARAEEIKKKYGTVAISNVLN
                     AEVIPVPGIGDRGTVPCSRERLARVMHARLSEILGLIADHLRKRQLDKIIGAGIVLTG
                     GTSATEGIVQLAEGIFKKPVRLGQPKQDVGLGDSLAKPSFSVGVGLLHYAAKTRKKSI
                     KETSTQGILSYIIAGTRRFIQIIKRYI"
     gene            1806..3251
                     /gene="ftsZ"
                     /locus_tag="PLT31_03640"
     CDS             1806..3251
                     /gene="ftsZ"
                     /locus_tag="PLT31_03640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPW94261.1"
                     /translation="MSENNYINIEGKSHILAAENFSGNAKIKVVGVGGAGGNAVNRMI
                     KMNIAGVDFLAINTDALALEHSLASEKIAIGQKITKSLGAGARPDVGRKAIMEDKETV
                     AEALKGCDLVFIAAGMGGGTGTGAASVVAEIAKSQGILTVAVVSLPFKFEGPVRSRNA
                     ANGISALRDTVDTIIVINNQKIMEVIDKTTTTEQAFATVDQVLGNAVRGVCDIMLHHG
                     NIHVDFNDVRSVMQDGGDALMGTGIAEGEDRALRAAENAINCPLLDDVNIEGASGVLV
                     NISHGENFTMQECSTAMEYIYSAVGEANDPSVIFGDITIPELGDKVSITVIATGFGFT
                     PLDKKTTSTSSVSASDPTMVMPKVNENNGRVVEKATPRPTANFCFMANQPTSAIEEVV
                     KPEPIAEPKTMDDYYPFSSFTGSELDVRSYSLEEEEQTESHFGVMDAEESLNTEKMNS
                     FDSDAFSLKNKSIDYNQPTIQREVNRHSEIF"
     gene            complement(3342..3869)
                     /locus_tag="PLT31_03645"
     CDS             complement(3342..3869)
                     /locus_tag="PLT31_03645"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPW94262.1"
                     /translation="MNINTLLLISHPDFSKSKINKALAEIASQKPNIIVRHLDEIQKD
                     NRYSLEEEFALLKSVSSIIWQFPIYWYNAPASLRNWQDQILSPIVYGPENFLIGKKLR
                     VVCSTGAAENTFSATGLNRFTAQQMLCPMQMTTNACRMEWQEPFITYGAGTITEDQIQ
                     EVAKKYSALLDQNTI"
     gene            4021..6399
                     /locus_tag="PLT31_03650"
     CDS             4021..6399
                     /locus_tag="PLT31_03650"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HPW94263.1"
                     /translation="MILKVKKRDGREKPFNIEKIAGAIAKALRASGELNERLREEEQQ
                     LSLIKINPEEIVDSTALNIAIAVVEHIESQGIDQPGIEDIQDAVEHVLVEQGYAETAK
                     RYILYRAERSRVRELNTRLMRTLHDITFSDAEKSDLKRENANIDGDTAMGTMLKYGSE
                     GAKHFYTMVMLKPEHSMAHQDGDIHIHDLDFYSLTMTCCQIDLNHLFEGGFNTGHGHL
                     RAPRDIRSYAALAAIAIQSNQNDQHGGQSIPNFEYALANGVRISYRKFYSANLIKALE
                     IMTDSEESKITEIVKKLHQELDALGIYPTLDPNPTFHENEARELVKNFSAEIVSKAQA
                     FAQKQAYQETDKTTFQAMEAFVHNLNSMHSRAGAQTPFSSINYGLCVEPEARMVMRNL
                     LLTTEEGLGAGETAIFPIQIFRVKEGINLNPGDPNYDLFKLACRVSAKRLFPNFSFQD
                     APYNLQYYKPGHPETEIAYMGCRTRVIGNIVDPTREITFGRGNLSFTSINLPRIAIKL
                     NGSQELFFKELDRILELVRDQLLERFEVQCRKKVKNFPFLMGQGVWIDSDQLDWNEEV
                     REVIKHGTLSIGFIGLAETLVALTGKHHGESEESQQLGLKIVKHMRDFCDAESERLKL
                     NFSLLATPAEGLSGRFVRMDRKRFGVKVGITDRDYYTNSFHVPVYYKTNALRKIELEA
                     PYHALTNAGHISYIELDGNPSHNLEAFEKIIRVMAKSGMGYGSVNHPLDRHPECSYTG
                     IIGDECPKCHAKESEGAKFERIRRITGYLVGTLERFNDAKKSEVKDRVKHGL"
     gene            6401..6940
                     /gene="nrdG"
                     /locus_tag="PLT31_03655"
     CDS             6401..6940
                     /gene="nrdG"
                     /locus_tag="PLT31_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HPW94264.1"
                     /translation="MSESEERVLRLRISGIEPESIVDGPGIRMTIFVQGCPHRCPHCH
                     NPQTHDFDGGHFLPIHEILEMIEENDLLDGITFSGGEPFSQAEELVPLAREIKERGYN
                     LVIYTGYLYEDLLASSSKIPAYLELLSFADILVDGPFIYAERTLDKPFKGSANQRVID
                     VQKSLSEGRVVLFSQNDEK"
     gene            7218..8141
                     /locus_tag="PLT31_03660"
     CDS             7218..8141
                     /locus_tag="PLT31_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HPW94265.1"
                     /translation="MKLKKMVLIAMALISSVFAQDKPYSGAELYSNETFMYGRYEARM
                     YMAAGSGLVSSMFLYYNDSWIGKGEPWVEIDIEVLGKSPSTFQSNIISGSLESKITSE
                     KHHNLSQPANATYHTYAMEWTPNYVAWFVDNVEVRRAAVGSSDEKNQVTNLNKEQSLR
                     FNLWSSTVAAWVGAWDENILPVNQFINWVKVYNYTPGQGPNGSDFTLSWVDNFESFDN
                     SRWGKGDWTFDENRVTMSKGNINVKDNTLILSITKVGQEGFTGTVPVDPEENTSIETP
                     KRNLEKRPSGIYEVKTFDLKGRYQGLQKIER"
     gene            8160..9503
                     /locus_tag="PLT31_03665"
     CDS             8160..9503
                     /locus_tag="PLT31_03665"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPW94266.1"
                     /translation="MKQIIVETLLNQKHRTGEQLPSVRQLMTSFKASSGTVQRALKEL
                     CSQGIIFSIPSKGYFWGNRTAFSNHPPVIQSPIELLHNRFMLDWRSGAFSHDRTLPSL
                     KELKARYHVSSSLLLKYLSQELQSGFLGRIGKSRFFINSTFQQETKTEVLLITRCTQW
                     GAFAPESEREVDFIRLVYRGAASRRLKLRLLGYNEETNQWIDRNGVVCHLEERKNTIG
                     AIVSTLLIQKPKPFLSALSHLSVPVSIWWEHPQEGFHSLFKKQPLWTFFNSTFGSVPG
                     RMMGRFLLEKGIQKVVYISPYHASSWSQDRLTGLQESGLVVLPFTDSTNASPWDYRER
                     ARQNGPKNTVELRARDLLRQRLKKMISKAPTTEAWVCVNDEVAAIIMELFEQKKINKK
                     PYIIGFDNSSESYLLQFDSYDFNTEALVLQMFYHLNSDSLLASHLVEIPGSLVEK"
     gene            complement(9507..9827)
                     /locus_tag="PLT31_03670"
     CDS             complement(9507..9827)
                     /locus_tag="PLT31_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020046532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPW94267.1"
                     /translation="MPQNIINLENHLSYQAESVVSREIIRKKEGTVTLFAFDKGQGLS
                     EHKAPFDALVQVVDGEADIFIGGEAHRLHAGELIIMPANIPHALQAVTAFKMILTMIR
                     QTQE"
     gene            9976..>11672
                     /locus_tag="PLT31_03675"
     CDS             9976..>11672
                     /locus_tag="PLT31_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HPW94268.1"
                     /translation="MPNLTLSDLLSPKGEPVLADNLLESFLTWVEDHHIDLYQAQEEA
                     ILELFEGKNVILNTPTGSGKSLVALALHFYSLSNGRRSVYTCPIKALVNEKWMALCKE
                     FGPENVGLSTGDATVNRDAPILCCTAEILSNMALSEGESLSIVDVVMDEFHYYSDRER
                     GVAWQIPLITLPQSRFLLMSATLGNTSFFESELLKHTGLECVTVRSMNRPVPLEFSYS
                     DQELSNTVQKLINSGKGPVYVVHFTQADASKNAQNFMSLDLCDKEEKLQIQKAIAETR
                     FSSPYGPDIKRWLRQGIGLHHAGLLPKYRILVEKLAQKGLLKVICGTDTLGVGVNVPI
                     RTVLFTQLCKYSGDKTAILSVRDFHQVAGRAGRKGFDDVGYVVAQAPEHVIENTKLAA
                     KASQSGRKFAKKQPPEFGYVPWDESTFRKLIDSPPEPLVSNFQVSHSMLLNILSRKTD
                     GCLAMRRLIEDSHETPISKKKLKKRAFQLFRALVEKEIISIIHPILPGQSKIAINMDL
                     QDDFSMNQPLSLFMVDSLPLLNSESPEYALDVISLCESILENPDLIFLLGDFMGHHI"
BASE COUNT         3668 a         2019 c         2392 g         3593 t
ORIGIN      
        1 tctgatgtaa atatagattc catcaaaaaa caactgctga cgttaccttt agttcattct
       61 gcagtggttt ctacatcgtt tccttcgacg ctagaaattg tattagaaga agccacccct
      121 ttaatggttt cttttgataa aaataaatgg aaagtctttt ctgaaagagc aaaggtttta
      181 ccaacagcaa ttttatcggc ttaccagtta ccagtagtgg atttgatgga tgaagaagag
      241 atgaaacaaa ttggaacttt tctaagtgca atgaaggcaa aagatgagtt tctctatcga
      301 aaagtatcgc agataggtgt taataaaaaa gaatatgcga tagaagtgtt ttttaaagat
      361 gttgaattta aaacgctgtt tcctttaagc ggttgggaag aaaaacagtt tgatcagtat
      421 aaacaattaa tacaaggttt tacttccgac ttaaaaacag caagtattgt ggatatgcgt
      481 tttaatgggt ttgcctatat aaaaccttat gaaaagaggt atagtgatgg atgataaaaa
      541 taaaatgacc gtaaataaag aaaacatcat tgttggactt gatattggtg gctccaaaat
      601 agctgttttt attggtttgc aagaaaatca gaatcaagta cgtgttcttg gttttggtgt
      661 aaaggaattg aaaaagaatc cgatttctga tttagatttt ttaatttcat ctataggtag
      721 tgccgtaaaa gaagccgaaa ccatgactgg ttttgatgta aaagaagttt atgttggtgt
      781 cgcaggtgat tacatctcct gtttcaaatc gaaaggcgga gtcactcttg acccttccaa
      841 aaatgaagtc aaagaatctc atgtggcggc ggtaattaag catgcaagca cattaccttc
      901 taccgcaggc gaaatacttc atattttccc aagaaattat tctttagatg atgttcaagg
      961 aataaaaaat ccaataggaa tgagtggtgt tcgtttagac gctgaagtac aaattgtcgc
     1021 tgctaaaaca ttgaatttgc agaatcataa aaagagcgtt gaaaaggcag gctttgaaat
     1081 aaaagcgatg gttttagaac cattagcagc agcttatgcc attttacacg atgaagaacg
     1141 agagctcggt gtcgctatta tagacattgg ggccacttct gctgatttag ctgtttttta
     1201 tgaagatgct gtatgctatt ctgtttctct tgatttagct ggaaacatca tcacgagtga
     1261 tattgctaaa agctttgatt tgcctatttc aattgctcgt gctgaagaaa taaagaaaaa
     1321 atatggaacg gtagccattt ctaatgtgtt aaacgcagaa gtcattcctg ttccaggcat
     1381 tggtgatcga gggactgttc cttgttctag agaacgttta gcaagagtaa tgcatgctcg
     1441 cttgagtgaa atattagggt taatcgctga tcacttaaga aagcgtcaat tagataaaat
     1501 cattggagca ggaatcgttt taacaggtgg cacaagtgct acagaaggaa ttgtacaact
     1561 agcagaaggt attttcaaaa aaccagttcg tttaggacaa cccaaacaag atgtggggct
     1621 tggagattct ttggctaaac cgtcgttttc agtaggcgtg ggcttacttc actatgctgc
     1681 taaaacgagg aaaaaaagta taaaagagac gagcacgcag gggattctaa gttacataat
     1741 agcaggaact cgtcgcttta tccagattat aaaaagatac atctaaactc aatagggaga
     1801 tcaatatgag tgaaaacaac tacatcaaca tagaaggcaa gtctcacata cttgctgcag
     1861 aaaactttag cggcaacgct aaaatcaaag tcgtcggtgt cggtggtgca ggtggaaacg
     1921 ctgtaaaccg catgatcaaa atgaatatcg ctggagttga ttttctagcg atcaataccg
     1981 atgcactagc tttagaacat agtttagcta gtgaaaaaat tgcgatcgga caaaagatta
     2041 caaaatcttt aggagcaggt gctcgtccag atgtcggtcg taaggcgatc atggaagata
     2101 aagaaacggt ggctgaagcg ctaaaaggtt gtgaccttgt gtttattgct gctggcatgg
     2161 gtggcggtac aggtactgga gctgcttctg tcgttgcaga aattgcaaaa agtcagggca
     2221 ttttaactgt ggccgttgtc tctcttccat ttaagttcga aggccctgtg cgttctcgta
     2281 acgcagcgaa cggaatctct gctttgcgag atactgtgga taccattatt gtgattaaca
     2341 accaaaaaat tatggaagtc attgataaga cgacgactac agagcaagcc tttgccactg
     2401 tagatcaggt tcttggaaat gccgtgcgtg gtgtttgtga tatcatgctt catcatggaa
     2461 atattcatgt tgattttaat gatgtgcgtt ccgtgatgca agatggcggt gatgcgttaa
     2521 tgggtacagg tattgcagag ggagaagacc gtgcattacg cgctgcagaa aatgcaatta
     2581 attgcccact cctagatgat gttaatattg aaggtgcttc aggtgtttta gtgaatattt
     2641 ctcatggtga aaactttacg atgcaggaat gttcaacagc aatggaatac atctatagtg
     2701 ccgttggtga agccaatgat ccatctgtca tttttggtga tattaccatt ccagagttag
     2761 gagataaggt cagtattaca gtcattgcca ctggctttgg ttttactcca ttggacaaaa
     2821 aaaccacttc tacttcttca gtcagtgcaa gtgacccaac aatggtaatg cctaaggtca
     2881 atgagaacaa cggtcgtgtc gttgaaaaag caacaccacg tccaacggct aatttttgct
     2941 tcatggctaa tcaaccaacg tcagcaatag aagaggtcgt gaaaccagag cctatagctg
     3001 aacccaaaac catggatgat tattatccgt tcagttcttt tactggatca gaacttgatg
     3061 ttagatctta tagtttagaa gaagaggaac aaactgaatc tcattttgga gtcatggatg
     3121 cagaggaatc cttaaataca gaaaaaatga acagctttga ttcagatgca ttttccttaa
     3181 aaaataaatc aatagattac aatcaaccga cgatacaacg tgaagtaaac agacattctg
     3241 aaatattcta acacaacccg aaaacatcaa accccttgat gttttcacca caacaaaaaa
     3301 gactatcgtg gttctccctc catggtagtc tttttttgct tttaaatagt gttttgatct
     3361 agtaaagcgg aatatttctt agccacttct tggatttgat cctcagtaat ggtgcctgct
     3421 ccgtaagtaa taaagggctc ttgccattcc atacgacaag cgttggtggt catttgcata
     3481 gggcagagca tttgttgtgc agtaaaacga ttcaatccag tggctgaaaa ggtgttttcc
     3541 gctgcgccag tactacaaac tacgcgtagt tttttcccta tcaaaaagtt ttctggccca
     3601 taaacaatag gactaagaat ttgatcctgc cagtttcgaa gagatgcggg ggcattatac
     3661 caatagatag ggaattgcca aataatggat gaaacgcttt ttaataaagc aaattcttct
     3721 tctagagaat aacgattatc tttttgtatt tcgtcaaggt gccttacgat aatattaggc
     3781 ttttgactag cgatttcagc taacgcttta ttaatcttag acttagaaaa gtcggggtga
     3841 gaaattaaaa gtagtgtgtt tatattcata ctacacaatg taaaaaaaat aaatggatag
     3901 atattttatt taaaggttgt tttattatct tgtcattcca tatattgtgt taatgaaatg
     3961 ataatgcctt aaaaatatgc aatatgttgt gaaaaaggca taaaataaat ggatggaacc
     4021 atgattttaa aagtgaaaaa acgcgatggt cgtgaaaagc cctttaatat agagaagatt
     4081 gcaggtgcaa ttgctaaggc acttcgtgct tcaggtgaat taaatgaacg tcttcgagaa
     4141 gaggagcaac agcttagttt aattaaaata aatccagaag agatagtaga ctccacagca
     4201 ttaaatattg ctatcgctgt tgtagaacat attgagtctc aagggataga tcaacctggt
     4261 attgaggata tccaagatgc ggtcgaacat gttttagtcg aacaaggtta tgcagaaaca
     4321 gccaaacgct atattctata tagggcagaa cgctctcgag ttcgagaact caatacacga
     4381 ttaatgcgta ctttacatga tatcactttt agtgatgctg aaaagtcaga cctaaaaaga
     4441 gaaaatgcaa atattgatgg cgataccgct atgggaacta tgcttaagta tggttctgaa
     4501 ggagcgaagc atttttatac catggttatg ctaaagcctg agcattctat ggcgcatcag
     4561 gatggagata tccatatcca tgatttagat ttttattcct tgacaatgac ttgctgtcaa
     4621 atcgatttaa atcatttatt tgaaggtggt tttaatacag gtcacggtca tcttcgtgca
     4681 ccaagagata ttcggagtta tgccgcttta gctgcaattg caattcaaag taatcagaat
     4741 gaccaacatg gtgggcaatc aattcccaat tttgaatatg ctttagccaa tggcgttcgt
     4801 atttcttatc gcaagtttta ttctgcaaac ttaataaaag ctttagaaat catgacggat
     4861 tcagaagaat caaaaattac tgagatcgtt aagaagcttc atcaagaatt agacgcttta
     4921 ggtatatatc ccactttaga tccgaatcct acatttcatg aaaatgaagc tcgcgaatta
     4981 gttaaaaatt tttcggctga gatcgtttct aaggcgcagg cctttgctca aaaacaagct
     5041 tatcaagaaa cagataaaac cactttccaa gcgatggaag cgtttgtcca taatttaaat
     5101 agcatgcata gtcgtgctgg agcgcaaacc ccattttcaa gcattaacta tgggctctgc
     5161 gtagaaccag aagctcgaat ggtgatgcgt aatcttttac tcacaacaga agaggggtta
     5221 ggtgctggag aaacagccat tttcccaata cagatcttta gagttaaaga aggaatcaac
     5281 ttaaatccag gcgatccaaa ttatgactta tttaagctcg cttgtcgtgt aagcgctaaa
     5341 cgcttgttcc ctaacttcag tttccaagat gctccttata atcttcagta ttataaacca
     5401 ggtcatcctg aaaccgaaat cgcatacatg ggatgtagaa ctcgtgtgat tggtaatatt
     5461 gtggatccca caagagaaat tactttcgga cgcggaaact taagctttac atccattaac
     5521 ttgcctcgta tagcaattaa attaaatgga agtcaggaac tcttttttaa agaattagat
     5581 cgaatcttag aactagttcg agatcagttg ttagaacgtt ttgaagtcca gtgccgtaaa
     5641 aaagtgaaga acttcccatt cttaatgggg caaggcgttt ggattgattc agatcaatta
     5701 gattggaacg aagaggttcg tgaagtcatt aagcatggaa cgctttccat tggttttatt
     5761 ggacttgcag aaacgctagt tgctttaact ggtaaacacc atggagaatc agaagaatca
     5821 cagcaattag gcttaaaaat agtgaagcat atgcgtgatt tttgtgatgc cgaaagcgaa
     5881 cgattgaaac tcaatttctc tttactagca acacctgcag aaggtctttc tggacgattt
     5941 gttcgaatgg atcgcaaacg ctttggagtc aaagtcggca ttacggatag agattattac
     6001 accaattcat ttcatgtgcc tgtgtattat aaaacaaatg ctcttcgtaa gattgaactt
     6061 gaagctcctt atcacgcgct tacgaatgca ggtcatatca gttatattga attggatgga
     6121 aatcctagcc ataatttaga agcgtttgaa aaaattattc gagtcatggc aaaatcagga
     6181 atgggttacg gttctgtgaa tcatccttta gatcgtcatc cagaatgttc ttacacaggt
     6241 attattggtg atgaatgtcc taagtgtcat gccaaagaat ctgagggtgc aaaatttgaa
     6301 cgcatccgtc gaattacggg ttatttagta ggtaccttag aacgttttaa tgacgccaaa
     6361 aaatcagagg ttaaagatcg agtcaagcat ggactttaat atgtcagaat cagaagagcg
     6421 agtcttaaga cttcgtattt ctggaattga gccagagtcc atcgtcgatg ggcctggtat
     6481 tcgaatgact atttttgtcc aaggttgtcc gcaccgttgc cctcattgcc ataatcctca
     6541 gacgcatgat ttcgatggag ggcatttttt gcctatccat gaaattttag aaatgataga
     6601 agaaaatgat cttttagatg gcataacttt tagtggagga gaaccatttt ctcaagctga
     6661 agagttagtt ccattagctc gtgaaataaa agaacgtggc tataatttag tcatctacac
     6721 aggctattta tatgaagatt tgttagcctc ttcttctaaa atccctgctt acctagaatt
     6781 gctctctttt gctgatatac ttgttgacgg cccttttatt tacgccgaaa gaacattaga
     6841 taaacctttt aagggatctg caaatcagag agtgattgat gttcaaaaaa gtttatcgga
     6901 aggacgtgta gttctttttt ctcaaaatga tgaaaagtaa ggacttatct tttttattta
     6961 ataaagtcct gttttatctt aaattccttg atttttgtct tttttaatcc attttccgag
     7021 ttgtttgttt gaaaatgatc ttttgattgg ttgccaaaaa aaacaattaa aaatagatac
     7081 agttgataca gttatatgtt ttgaagctgt tattcaggat tcagcttctg atgtaataac
     7141 ttattttttt tacgtggtta aggatacttt ctatgctcta atcttttaga gtgagttttc
     7201 aaaacaagga tctttttatg aaactaaaaa aaatggtatt aatcgctatg gccctaattt
     7261 catctgtatt tgcacaagac aagccttatt caggtgctga gttatatagc aatgaaactt
     7321 ttatgtacgg caggtatgaa gcaagaatgt atatggccgc aggtagtggt ttagtaagtt
     7381 ctatgttttt gtattacaat gactcatgga taggtaaagg agaaccttgg gtagaaatag
     7441 atatagaagt gttaggtaaa tcaccaagca cgtttcaatc aaacataatc tctgggtctt
     7501 tggagtctaa gatcaccagt gaaaagcatc acaatctctc ccagccagcc aatgccactt
     7561 atcatactta cgcgatggaa tggacgccta attatgtagc ttggtttgta gataatgtag
     7621 aagtcagaag agctgcagta ggatcgagtg atgaaaaaaa tcaagtgact aatttgaata
     7681 aagaacaaag tcttcgtttt aacctttggt cttctactgt tgcagcttgg gtaggtgctt
     7741 gggatgaaaa tatattacct gtaaatcaat ttattaactg ggttaaggtt tataattata
     7801 ctcctgggca ggggcctaat ggtagtgatt ttaccttgtc ttgggtggat aattttgaat
     7861 cttttgataa ttctcgttgg ggtaaaggag actggacttt cgatgaaaat cgagttacaa
     7921 tgagtaaggg taatatcaac gttaaagata acactttgat cttaagtatt acaaaagtgg
     7981 gccaagaagg ttttacagga acagtccctg ttgatccaga ggagaacaca agtattgaga
     8041 ctccaaaaag aaatcttgaa aaacgtcctt ctggcattta tgaagtaaaa acctttgatc
     8101 taaaaggtcg ttatcaggga ttacaaaaaa tagagcgctg atttatgtaa ttttacataa
     8161 tgaagcaaat cattgttgaa acattattga atcaaaagca tcgaacagga gagcaattgc
     8221 cttctgttcg tcaattaatg acttcattca aagcatcatc ggggactgtt cagcgggcat
     8281 taaaagaact ttgcagtcag ggaatcattt tttcgattcc atcaaaggga tatttttggg
     8341 gtaatcgcac tgctttttct aatcatcctc cagtaattca atctcctata gaattattgc
     8401 acaatcgatt tatgctcgat tggagatcgg gagctttttc tcatgatcga acattacctt
     8461 ccttaaaaga attaaaagct cgctatcatg tctcttcctc tttgctctta aagtatttat
     8521 cgcaagaact acaatctggc tttcttggcc gaattggaaa aagtcgattt tttatcaatt
     8581 ccacttttca acaagaaaca aaaacagaag ttttactaat tacacgttgt actcaatggg
     8641 gcgcttttgc tccagaaagt gaacgagaag tagatttcat tcgtttagtg tatcgaggag
     8701 ccgcttctcg gcggctaaaa ctacgcctat taggctataa tgaagaaaca aatcaatgga
     8761 ttgatcgaaa tggagtggtc tgtcatcttg aagaaagaaa aaatacgatt ggcgccatcg
     8821 tttcaacgct tttaattcaa aaaccaaaac catttctctc cgctttaagt catctttcgg
     8881 ttcctgtatc tatttggtgg gaacaccctc aagaaggatt ccattcgcta tttaaaaagc
     8941 agcctttatg gacgtttttt aattcgactt ttggttctgt accaggccgc atgatgggac
     9001 gatttttatt agagaagggt attcagaaag tcgtttatat ctctccatac catgccagtt
     9061 cttggtctca agatcgcctc acaggacttc aagaaagtgg tttagttgtt ttgccattca
     9121 cagattctac taatgcaagc ccttgggatt acagagaacg ggcgcgtcaa aatggtccta
     9181 aaaatacagt agaattaaga gccagagatt tattacgcca aaggctgaaa aaaatgattt
     9241 ctaaagctcc taccacagaa gcatgggtct gcgtgaatga tgaagtggct gctataataa
     9301 tggaactttt tgaacaaaag aaaataaata aaaagcctta tattataggc tttgataatt
     9361 cttctgaaag ctatctattg caatttgatt cttatgattt taacacagaa gcattagtgc
     9421 tacaaatgtt ttatcatctg aattccgatt cgcttttagc aagtcacctt gtagaaatac
     9481 caggctcctt agtggaaaaa tgatttttat tcctgagtct gtcgaatcat cgttaaaatc
     9541 attttaaaag cagtgaccgc ttgtaatgca tgtggaatgt tcgctggcat aatgatgagc
     9601 tctccagcgt gtaagcgatg agcttctcct ccaataaaaa tatcggcttc tccatcgact
     9661 acttgaacaa gagcatcaaa cggtgcttta tgttcactta aaccttgccc tttatcaaaa
     9721 gcaaataaag tgaccgttcc ttcttttttg cgaatgattt ctcgactcac tacggattcg
     9781 gcttgataag acaagtggtt ttctaagttg atgatgtttt gaggcatgag tcatccttta
     9841 tcgttttatt aacgatggct aaactattca ataatatatg aaatgaataa atacaaaagt
     9901 gcttaatatg taaagatttt gtataaaaaa cgaacgtttc ctttttcgta aagagagata
     9961 aattatcttt gttttatgcc taatttaacg cttagtgacc tactttctcc taaaggggaa
    10021 cctgttttag cagataactt gcttgaatca tttttaactt gggttgaaga tcatcatatt
    10081 gatttatacc aagctcaaga agaagctatt cttgaattat ttgaaggcaa aaatgtcatc
    10141 ttaaatacac ccacaggttc tggaaagtct ttagtcgcct tagctcttca cttttatagc
    10201 ttatctaacg gtcgtcgatc tgtttacact tgccctatta aagcacttgt gaatgaaaaa
    10261 tggatggcgc tctgtaaaga atttggtcct gaaaatgtag gcctatctac tggagatgca
    10321 acagtcaatc gagacgctcc tatattatgt tgcacggcag aaattctttc caatatggca
    10381 ttaagtgaag gggagtctct ttctatagta gatgtggtga tggatgaatt tcattactac
    10441 tctgatcgtg aacgcggggt ggcttggcaa atccctttaa ttacgttacc tcagtctcga
    10501 ttcctattaa tgtcagcgac tcttggaaat acttcctttt ttgaatcaga actattgaaa
    10561 catacagggc ttgagtgtgt tacggttcgt tctatgaatc gacccgtgcc tcttgagttt
    10621 tcttattctg accaagaact ttctaatact gttcaaaagc ttataaattc agggaaagga
    10681 cctgtttatg ttgttcactt tactcaagcc gatgcctcaa aaaatgcaca gaattttatg
    10741 agtctagatt tgtgtgataa agaggaaaaa ctacagatcc aaaaagccat tgcagaaaca
    10801 cgtttttcaa gcccctatgg ccctgacatc aaaagatggc ttcgtcaagg gattggttta
    10861 catcatgcag ggcttcttcc caaatacaga attcttgtag aaaagctcgc tcaaaagggc
    10921 ttgttaaaag tgatttgtgg aacagacact ttaggcgttg gcgttaatgt tcctattcgt
    10981 accgttcttt ttacacagct atgtaaatac agtggtgaca aaacggcgat actttctgta
    11041 agagattttc atcaagtggc agggcgtgca gggcgaaaag gttttgatga tgtaggctat
    11101 gtcgtggcgc aagctccaga gcacgttatt gagaacacaa agctagcggc aaaagcatct
    11161 cagagtgggc gaaaatttgc gaaaaaacaa ccgccagaat tcggctatgt cccttgggat
    11221 gaatcgacat ttcgtaagtt aatcgattcg cctccagagc ctcttgtctc taactttcaa
    11281 gtatctcaca gcatgcttct caatattctt tctcgaaaaa cagatgggtg tttagcgatg
    11341 aggcgcttaa tagaagattc gcatgaaacc cccatatcta aaaagaaact taaaaaaaga
    11401 gcgttccaat tatttcgagc gttagtagaa aaagaaatta taagtatcat tcatcctatc
    11461 cttcctgggc aatcgaaaat agcgattaat atggatttgc aagatgactt ttccatgaat
    11521 caaccgctat ctctttttat ggtggactct cttcctttat taaattcaga aagtcctgaa
    11581 tatgctttag atgtcatttc gctttgtgaa tcgattcttg aaaatccaga tttgattttt
    11641 ttactcggag atttcatggg acatcacata ag
//
LOCUS       DAOOBB010000048        11124 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_7457_length_11124_cov_4.333544, whole genome shotgun sequence.
ACCESSION   DAOOBB010000048 DAOOBB010000000
VERSION     DAOOBB010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 11124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11124
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7457_length_11124_cov_4.333544"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2635
                     /gene="ileS"
                     /locus_tag="PLT31_03680"
     CDS             <1..2635
                     /gene="ileS"
                     /locus_tag="PLT31_03680"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HPW94269.1"
                     /translation="ALATPLSNFETNQGYKDRQDPSLTLIFPIVSENSRFKDTSILVW
                     TTTPWTLPSNFAIAVGPDMEYSLVSHEDKKLWVATSRIQAYFKDAEVLDTCKGSDLLG
                     LHYEPLFRFSDAYATKEELEKRYQIYPADFVSTDDGAGAVHIAPSFGEEDFQLGASLG
                     LGLFDPLNEEGRFTEKVPLWQGLGAKEADKSIMAFLKEQGRVFRHDTLVHSYPHCWRT
                     GVPLLYRALKTWFLKIDGELTNAEGETKTLKQWMIDSNQQINWVPDHIKNGRFGKWLE
                     NARDWNISRNRFWGTPLPVWLSEDGEMIAVGSIQELEELSGVKLNDLHKHFVDPIVIK
                     KNGKEFRRTSEVFDCWFESGSMPYAQNHYPFENKEMVEASFPADFIAEGLDQTRGWFY
                     TLSVLSNALFQKPAFKNVIVNGIILAENGQKMSKSLRNYPDPNELIGRTGADAIRLFM
                     INSAALKAEDLRFSEEGVKNIVKQVMLPLWNAVSFFISNHNADAAKGQLDWKPGQALR
                     SENELDRWMLAVLQDLSEKVEQEMAAYRLYNVVPAIISAVDDLTNWYVRRSRRRFWKS
                     DNDGDKNAAYATMYKVLVDFSKILAPFLPMLSEEIYQLLVRETDPNAPVSVHLCEFPS
                     ADESLKDPRLVERIAMVRGMVEMGRIIRATNNVKNRMPIASMTVVPHGPIEKEVAENM
                     RELILEELNVREMKFLDDETALVKLSAKPNFLGIKAKGPEYSKNMKDIQAKLAKLNAE
                     EIRDLQEGKTIEFPFGCVGGDCLLIQRIVPEGMAVEANSHFTVALDLTVTDELKRACI
                     ARELVNRIQNRRKDEDYAITDKIEVTLYSSSDVFKKAVEENLSYIMGETQAINLIWTS
                     SDEGLNSNEADGEAFAFTTKR"
     gene            complement(2733..4757)
                     /locus_tag="PLT31_03685"
     CDS             complement(2733..4757)
                     /locus_tag="PLT31_03685"
                     /inference="COORDINATES: protein motif:HMM:NF015387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HPW94270.1"
                     /translation="MSKKINSMLTAVLLGVVSLSAQNVTVNLNEEYQTIRGFGGMVHN
                     TWQGGAGLSAEDAKIAFGAGEGQLGLTALRIPVNESKNDFGKELAVAKLAQSHGAIVY
                     ASPWNPPSNLRDGNRKIPESNWQAYVDHLNSFSAYMKDQGAPLYAISIQNEPDWCHEW
                     TCWSADELYKFTKNYGNQLRKNGTKVITAESFAYVKTLYDKILNDATALSNVDIIGAH
                     FYASTAASGDNFFQYSLADQKAKNQERWMTEHYTESQGSGNMWRAYIKTGDQDQQAKY
                     DTVRALDVGYEIHRGLVVGNFNQYTWWYIRRCYGLIMEKDFGNKLTIPSNEIGKVSKR
                     GYVLSQYARFVRPGAIRVGATAKPMNHVFASAYKKEDSVVVVLINRDAGATKTLNISV
                     PGAPSITSWTKYTTSATKNVSNDGEISATNGSFSVTLDKESIVTLVGTGPQIVIPQGP
                     YDSLNVPTIPGKIEAENYDVGGHNKAYYDEESENKGKAYREEGVDIEGNETDGFVVGY
                     TMAGEWMEYTLDVKKEGIYAFNARVSSGAENASFIMYMDSVAITDTIKVPQGADWTTY
                     GNIKGETKTLPAGRHILKIEVTGNYFNLDWIDFQDPSLVKINPSLELSISKTNEFEVF
                     NLSGKRVGSFVSTNLNNIKTQINDIVRHSGIYYVKSKTGTFCKRISIVKE"
     gene            complement(4836..6992)
                     /locus_tag="PLT31_03690"
     CDS             complement(4836..6992)
                     /locus_tag="PLT31_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HPW94271.1"
                     /translation="MNLPIRIALFGIIASVGVHIYADNPISSYHYLADPAATSDGETF
                     YILTDSDDPAGSNGYTIKSLYGFSSKDMKNWTDHGIIFEAKREYDNINDIWASGIDIA
                     PDGTFYIVYPDGGGGGVGLIGAKNFSGPWENPIPGNKKLINNWGGGLADCDGIGWCFD
                     PAIYFDTDGSGYFTFGGGSSDSRPAAENNNNIFNIYKFNSDMKGFDINSKVQLKVGGP
                     KAMEASYIHKLGSTYYLTYSTADLRIAYATSSKVTGPYTYQGIFMDNPNINGQNINAH
                     NNNHHGIAEFKGKWYAVYHDRRLVQASEHPASLGVANPEPAYHRSVSIDEFTFSGEKM
                     NKLVYTNEGPKQIANFDPYQTYPAITSSKQRNIRSRTDWTRGQPVKHVLTPYSTKESW
                     IRVSGVDFGSGATNFIVTAASVASGNKIEIRSGSVSGTLAGSCDLPQTSGWNAYETTE
                     CAVDGLKGVVDQLFLVFKGSKDSTMGVLEWEFSGAPSAPQTAYKGVAATIPGVIEAEN
                     FDDGGARKAYSDEESENQGDATMRATEGVDLVTIPEGTAVGYTVAGEWLEYTVNVTGT
                     TDKSFTARVASGSETSSFMLFLDDVAISDTIKIPAGADWDTYTEVTGQLNSLTEGEHV
                     LKILITGSYVNIDWIALGLKPTGFSNPLNLGALEAESTFRVFNVQGKQVGQFKASNRS
                     NITQKTQMVVDKSGVYLVKPQNDSKVYRVFVSKKGF"
     gene            complement(7176..8342)
                     /locus_tag="PLT31_03695"
     CDS             complement(7176..8342)
                     /locus_tag="PLT31_03695"
                     /inference="COORDINATES: protein motif:HMM:NF022251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94272.1"
                     /translation="MKKWVWFVAIFYSGLYVACSIGDDVSGSSFETENSIAFEVVLTN
                     KHPAAHTDVIIRPSDYVARSKNTAKTDLAQGIWNGKTDENGKITINNLPNGKYVIETR
                     NDSLKGVMSFELDSTDLKGVNLIMKKPGTVFGSVVLPPEYRRAKVGIVGLDYFALTDS
                     TGSFEFESLPADTFSIVALMESESSAFTQFASSSVAVVSGESTEINIGVNGKMDSVTV
                     VDSLFYEDFEDSTKGWYTSAANYATADLRAVLEEDRDELVAYFSYTNDSTNGWALMGN
                     SFDDVQDLSDLDSVSFMARGDGRVSLAFDHWDDVDSAFTDKAWIHIEINSSWTRYVVK
                     PSDFLKADSIGGNTGWDSVKKAVTNITFFGNNGKEFWLDDIVLYSTKRVLKGIE"
     gene            complement(8344..9171)
                     /locus_tag="PLT31_03700"
     CDS             complement(8344..9171)
                     /locus_tag="PLT31_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4423 domain-containing protein"
                     /protein_id="HPW94273.1"
                     /translation="MTNFSDVADYRDLLKSYYEQRKKEMPFYSYRMMGDKLGLDSSYL
                     YRVLQKKQHLPAHVIPAAKELLDVSGRSAEYFDLLVAAGISKSAEKKAELMAKAVALR
                     DVERHSLQQAELKFLENWWIPAVRAYLDLNGGVVNIKKIAKDLCPPITEEQAAEAIET
                     LKEIGLVKNVTSGRLVLSDAHLTVGGQEKSAAVRHFQKQVLFLASEALESVPVNERNI
                     STLTLSVDQACFEDLGEMLREFRRLVQKRVDESKRPDRVMQLSMAFYPVTKTNGKVG"
     gene            complement(9848..10930)
                     /locus_tag="PLT31_03705"
     CDS             complement(9848..10930)
                     /locus_tag="PLT31_03705"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPW94274.1"
                     /translation="MKRLSLSGDNLKLHLQSRAAFYGAVAKNSKNHGMIPVLWDTGDE
                     GEGNMTVIRRQKGTTGSIFDYEVLNSMLKAYGLDTLQGNSIDALVNLSTDTSNKSVLV
                     ETKASPDTSTTTTIRITPSIKDWSNYTAVSFLGYVDGTTHQVDTAYGFASIDLAVMSG
                     TDWAWDDVHSQELTMGQWAEFKYTLSKNIADESQAAKNQQILYIENPTSINALVINLY
                     SLGFEGEIRIDNVVLWKADGTKDTLETFNKKLPTTEGNVSNTELVATVISGPIKICAS
                     PIKTLSTKLRFTQNANQLNVSFESERSSKGTLVLMNSLGQVIVEKSISVEKGLNTVSL
                     KTGFRGVAFVQLRQQSSSSTAKVIIK"
     gene            complement(10943..>11124)
                     /locus_tag="PLT31_03710"
     CDS             complement(10943..>11124)
                     /locus_tag="PLT31_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94275.1"
                     /translation="LMTEDADWGKCYYYWGNYLSSTDAVHNTPASGFASPAWVDETLS
                     SVSTKFAAKQMPVVI"
BASE COUNT         3469 a         2342 c         1890 g         3423 t
ORIGIN      
        1 tgctcttgca acacctcttt caaattttga aacaaatcaa ggttataaag atcgtcaaga
       61 cccatcttta actttaatct tccctattgt ttcagaaaat tctcgcttta aggatacctc
      121 tatacttgtc tggacaacga ctccttggac tttgccatct aactttgcaa ttgctgttgg
      181 gcctgatatg gaatacagtc ttgtttccca tgaagataaa aaactctggg tcgccactag
      241 tcgtattcaa gcctacttta aagatgctga agtattagat acatgtaaag gatctgattt
      301 actcgggctc cactacgaac ctttattccg tttttcagat gcctacgcta ctaaagaaga
      361 gctcgaaaag cgctaccaga tatatcccgc tgatttcgta agcaccgatg acggcgctgg
      421 cgcggttcat attgcaccta gctttggtga agaagacttt caattaggag cctctcttgg
      481 gcttggcctt tttgatcctc ttaacgaaga aggtcgtttc acagaaaaag tacctctatg
      541 gcaaggattg ggcgcaaaag aagccgacaa atctattatg gctttcttaa aagaacaagg
      601 acgtgttttt agacatgata ctttagttca tagttatcct cattgctgga gaactggtgt
      661 tcctctttta tatagagcct taaaaacatg gttcttaaaa atagatggcg aactcactaa
      721 tgctgaaggc gaaaccaaaa cattaaaaca atggatgatt gattctaatc agcaaatcaa
      781 ctgggttcct gatcatatca aaaatggtcg tttcggaaaa tggttagaaa acgctagaga
      841 ctggaatatt tctcgcaacc gtttctgggg cactccactc cctgtctggc tctctgaaga
      901 cggggaaatg attgctgttg gttccatcca agagctcgaa gaactttctg gtgtaaaatt
      961 aaacgattta cacaaacact ttgtagaccc aattgtcatt aaaaagaatg gcaaagaatt
     1021 ccgccgcaca agcgaagttt tcgactgctg gtttgaatct ggctctatgc cttatgcaca
     1081 aaaccattat ccttttgaaa ataaagaaat ggtcgaagcc tctttccctg cagactttat
     1141 tgccgaaggc ctggatcaaa ctcgcggctg gttctatact ctatccgttc tctccaacgc
     1201 cctcttccaa aagcctgcgt ttaagaacgt cattgtaaac ggcattattc ttgctgaaaa
     1261 cgggcagaaa atgtctaaga gcctacgcaa ctacccagac cctaatgaac tcattgggcg
     1321 tacgggagcc gatgcgattc gcttatttat gattaattcg gctgctctaa aagcagaaga
     1381 tttacgcttc tcagaagaag gcgttaaaaa cattgttaag caggtaatgc tcccgctttg
     1441 gaatgctgtt agctttttca tcagcaacca caacgctgat gctgctaaag ggcaattaga
     1501 ctggaagcca gggcaagcgc ttcgtagcga aaatgaatta gaccgctgga tgcttgcagt
     1561 gcttcaagac ctttcagaaa aggtcgagca agagatggcg gcttatcgtt tgtataacgt
     1621 agttccagca attatctctg ctgtggatga tcttaccaac tggtatgttc gccgtagtcg
     1681 tcgtcgtttc tggaaaagtg ataacgatgg agataaaaat gctgcatatg ctaccatgta
     1741 taaagtcctt gtagatttca gcaaaatact tgctccattc ttacctatgc tttcagaaga
     1801 gatttaccag cttctcgttc gtgaaacaga tccaaatgct cctgtcagcg ttcatttgtg
     1861 tgaattccct agtgccgacg aatccttgaa agaccctcgt ttagtggagc gtattgccat
     1921 ggttcgcggg atggttgaaa tgggacgtat tattcgcgcc acaaataacg tgaagaatcg
     1981 tatgcctatt gcaagcatga ctgttgttcc tcacggccca atcgaaaaag aagtcgctga
     2041 aaatatgcgt gagcttattc ttgaagagtt aaacgttcgc gaaatgaaat ttcttgatga
     2101 tgaaaccgct cttgtaaaac tttctgcaaa accaaacttc ctcggtatta aagcaaaagg
     2161 ccctgaatat tctaagaaca tgaaggatat tcaagcaaaa ctcgctaaac tcaatgccga
     2221 agagattcgt gatttacaag aaggtaaaac tattgaattc ccatttggtt gcgtcggtgg
     2281 agactgtttg ttaattcaac gcatcgtacc tgaaggcatg gcagttgagg caaattctca
     2341 ttttactgtt gctcttgatt taactgtcac agatgagctc aaacgcgctt gcattgcaag
     2401 agaacttgtc aatcgtattc aaaatcgtcg taaagacgaa gactatgcca ttacagataa
     2461 aatcgaagta actctctatt cttcgtctga tgtatttaag aaagcagtag aggagaatct
     2521 ttcttatatc atgggtgaaa cgcaagcgat taatttaata tggacttcaa gtgatgaagg
     2581 ccttaattct aatgaagcag atggagaagc atttgctttt accacaaaaa ggtaaaatac
     2641 ttcttaattg ttattaaaaa gaagcggtgg attaattatc caccgcttct tttattgaga
     2701 gagagatcta ttataataag aaacttatta ttctattctt ttacaataga aatacgttta
     2761 cagaaagttc ctgtttttga cttcacataa taaatgcctg aatgtcgaac gatatcattt
     2821 atttgtgttt ttatgttatt taagtttgta gaaacaaaag aacctactct ctttccacta
     2881 agattaaata cttcaaattc atttgtcttt gatatggata attcaagtga tggatttatt
     2941 ttaaccaaag aaggatcttg aaaatctatc caatctaaat taaaataatt tccagtcact
     3001 tctatcttta agatgtggcg accagcgggc agtgtttttg tctctccttt tatatttcca
     3061 taagtagtcc aatcagctcc ctgagggact tttatggtgt ctgtaatagc tacagaatcc
     3121 atgtacatta taaacgaagc gttttctgca ccagaagaaa cacgagcatt aaacgcatat
     3181 atcccctctt tttttacatc aagagtgtat tccatccact ctcctgccat cgtataaccc
     3241 acaacaaaac catcggtttc attcccttca atatcgacgc cttcctcacg ataagccttg
     3301 cctttatttt ctgattcttc atcataataa gctttattat ggccaccaac atcataattt
     3361 tctgcttcta tttttcctgg aatagtagga acattcaaag aatcataagg gccttgagga
     3421 attacaattt gaggacctgt acccaccaaa gtgacaatac tttctttatc taaagtcaca
     3481 gaaaaactgc cgttagttgc agaaatttct ccatcattac ttacattttt tgttgcagaa
     3541 gtagtgtact tagtccaaga agtaatggat ggcgctcctg gaacggatat attcaaagtc
     3601 ttggtagcgc ctgcatctcg gttaattaag accacaacaa cactatcttc ttttttatat
     3661 gcagaagcaa agacgtgatt cattggctta gcggttgcac caactcttat tgcccctgga
     3721 cgaacgaaac gagcgtattg agaaaggaca taacctctct tagaaacctt tcctatttca
     3781 ttgctaggaa tagtaagctt atttccaaag tccttttcca taatcaaacc ataacagcga
     3841 cgaatatacc accatgtgta ttgattaaag tttccaacaa ccaaacctct atgaatttca
     3901 taaccaacat ctaaagctct cacagtgtca tatttagctt gctgatcttg gtctcctgtt
     3961 ttaatgtaag cacgccacat attgccgcta ccttgacttt ctgtgtaatg ttcagtcatc
     4021 cagcgttctt gatttttagc tttttgatct gctaaggagt attgaaagaa attatcccct
     4081 gaagcagctg tactcgcata aaaatgagct ccaataatat ccacattact cagagcagtt
     4141 gcatcattca atattttatc atacaaggtt ttgacatagg caaaagactc tgcagtaatg
     4201 acctttgttc cattcttacg tagttgatta ccatagtttt ttgtgaattt ataaagttcg
     4261 tcagcactcc aacatgtcca ttcatggcac caatcaggtt cattttgaat agaaatagca
     4321 tatagaggag ccccttgatc tttcatgtac gctgaaaaac tatttaagtg atccacataa
     4381 gcctgccaat tactttctgg aattttccta tttccatctc gcaaattcga aggagggttc
     4441 catggtgagg catagacaat tgcaccatga gattgagcta atttggctac agcaagttct
     4501 ttgccaaaat catttttact ttcattgact ggaatacgta aagcagttaa gcctaattga
     4561 ccttcacctg caccaaaagc gatttttgca tcttctgcag aaaggcctgc tccaccttgc
     4621 caagtattgt gaaccattcc accaaaacca cgaatagttt ggtattcttc atttaagttc
     4681 accgttacat tttgagctga tagtgatact acaccaagta ataccgctgt aagcattgaa
     4741 tttatttttt ttgacatcat aaacacatcc ttataaattg atacgacaaa aggagccttg
     4801 cttaagcata gactcctttc aaaacctaga accaattaaa accctttttt agaaacaaat
     4861 actcgataga cttttgaatc attttgaggc ttaactaaat agactcccga tttatcaact
     4921 accatttgtg ttttctgtgt aatattggat cgatttgaag ccttaaattg ccccacttgc
     4981 tttccttgaa cgttaaacac tctaaatgta ctttctgctt ctagagcacc taaattcaat
     5041 ggatttgaga agcctgttgg tttcaagcca agagcaatcc agtcaatatt tacataacta
     5101 cccgtaatta gaatcttcaa aacgtgttcc ccttcagtga gagaatttaa ttgaccagtg
     5161 acttctgtat aggtatccca atcggctcct gcaggaattt tgattgtatc tgagatagcc
     5221 acatcatcta aaaagagcat gaaggatgat gtttctgaac cagaagcaac gcgagcagta
     5281 aatgatttgt cagtagtacc agttacgttg acagtatatt caagccattc acctgcaaca
     5341 gtgtaaccaa cagcggttcc ttctggaata gtaacaaggt ctacgccttc agtagcacgc
     5401 atggtagcgt caccttggtt ttctgactct tcatcactat aagcttttct ggcgccgcca
     5461 tcatcaaaat tttcagcttc aattacacct ggaatagtcg ctgccacacc tttataggct
     5521 gtttgtggtg cactaggagc tccagagaat tcccattcta aaacgcccat agtggaatct
     5581 ttagatcctt taaatacaag gaacaattga tctactacgc cttttaatcc atctacagca
     5641 cattcagttg tttcataagc attccagcca cttgtttgtg gaaggtcaca agaaccagcc
     5701 aaagtgccgc ttacgctacc tgaacgtatt tcaattttat taccgctagc aacacttgca
     5761 gcagtgacaa taaagtttgt agctccagat ccaaaatcaa ctccagagac gcgaatccaa
     5821 gattcctttg tagaataagg agtaagtaca tgctttacag gttgtccacg agtccaatcg
     5881 gtacgactac gaatattacg ttgcttacta ctagtaatcg caggataagt ttgataagga
     5941 tcaaaattgg caatctgttt tgggccttca tttgtatata ctaacttatt cattttttca
     6001 ccactgaaag taaactcatc aatacttaca ctacggtgat aggcaggctc tggatttgca
     6061 actcctaaag aagcgggatg ttcagaagct tgaactaaac gacggtcatg atacacagca
     6121 taccattttc ctttgaattc tgctatacca tggtgattat tattatgtgc attgatgttt
     6181 tgaccgttaa tatttggatt atccataaag atgccttgat aagtataagg ccccgttact
     6241 ttactagatg tagcataagc aattctcaag tcagctgtac tataggttaa ataatatgta
     6301 cttccaagct tatgaatata ggaagcttcc attgctttag ggccaccaac ctttaactgt
     6361 acttttgaat tgatatcaaa tcccttcata tcggaattga atttgtaaat attaaagata
     6421 ttgttattgt tttctgcagc agggcgagaa tcactactac ccccaccaaa agtaaaatag
     6481 ccgcttccat cagtatcaaa atagatagca gggtcaaaac accacccaat accatcacaa
     6541 tccgctaaac cgccacccca gttattgatc aacttcttat tacctggaat aggattttcc
     6601 catggaccac taaaattttt ggcacctatt aaaccgacgc caccgccacc accatctgga
     6661 tagacaatgt aaaaggtacc atctggggca atatcaatac cagatgccca aatatcattg
     6721 atattatcat attcacgttt cgcttcgaag ataataccat gatctgtcca gtttttcatg
     6781 tctttactgc taaatccata gagcgactta atcgtatatc catttgagcc agctggatca
     6841 tcagaatctg taaggatata gaatgtttca ccatctgaag ttgcagctgg atcagctaaa
     6901 taatgataac tgctaattgg attatctgca taaatatgaa cgcccacact agcaatgatt
     6961 ccaaataagg caattcttat tggtaaattc attttgactc ctttttttaa tccctaatat
     7021 ccaaaaggta accgatacaa taaaatctat tttcaaaccc ccattttctt aactgttcaa
     7081 cgatagttct tatggataga ttgtccataa aaaaacaccc aggaaagaaa atcctgagtg
     7141 ttattcaact caaaaaaatg aagcaatgac ttatgctatt caattccttt taaaactcgc
     7201 tttgtactgt ataatacaat atcatctaac caaaattctt ttccattatt gccaaaaaag
     7261 gtgatattcg taactgcttt ttttactgaa tcccaaccag tattaccacc aatgctatct
     7321 gcttttaaga aatctgatgg ctttactaca taacgagtcc aagaggaatt aatttctata
     7381 tgaatccaag ctttatcagt aaaggcacta tcgacgtcat cccaatggtc aaaagctaaa
     7441 gaaacacgac catctcctcg agccataaag ctcaccgaat ccaaatcact aaggtcttgt
     7501 acatcatcaa aagaattgcc cattaaagcc cagccatttg tagaatcgtt agtatagcta
     7561 aaataagcta ctaattcgtc tctatcttct tctaatacag ctcttagatc tgctgtagca
     7621 taatttgccg ctgaagtata ccaaccttta gtactgtctt caaaatcttc ataaaataag
     7681 gaatccacaa cagtaaccga atccattttt ccattcacgc ctatattgat ctctgtcgat
     7741 tcacctgaga ctacagccac tgatgatgag gcaaattgtg taaaagcact agattcactt
     7801 tccatcaaag caacaataga gaatgtatct gcagggagag actcaaattc gaaagaccct
     7861 gtagaatctg ttaatgcgaa ataatctaaa ccaacgattc caactttagc tcgcctatat
     7921 tctggaggta aaacaacaga gccaaaaacc gttccaggtt tcttcattat aagattcact
     7981 ccctttagat ctgtagaatc tagttcaaaa ctcattacgc cttttaggga gtcatttctc
     8041 gtttcaatta catattttcc attaggtaaa ttattaattg tgattttacc gttttcatcg
     8101 gtttttccat tccaaatccc ctgagcaaga tctgtttttg ctgtgttttt tgatcttgca
     8161 acgtaatcag agggacgaat gatcacgtct gtatgagctg caggatgttt atttgttaaa
     8221 accacctcaa aagcaatgga attttcggtc tcaaatgatg agccagaaac atcatcgcct
     8281 atagagcaag ctacgtacag cccgctataa aagattgcca caaaccacac ccattttttc
     8341 atcttagcct accttcccat tagtctttgt cacaggataa aaagccatag aaagctgcat
     8401 cactcgatct gggcgtttag attcatcaac acgcttttga actaaacgtc taaattccct
     8461 taacatttct ccgagatctt caaaacaagc ctgatctaca gataaggtta aagtggagat
     8521 atttctctcg ttcactggaa cactttctaa agcttcactt gctaaaaata acacttgttt
     8581 ttgaaaatgt cgcactgcag ctgatttttc ttgtccgcca accgttaaat gggcatctga
     8641 aagcactaat cgaccagaag tcacgttttt tactaggcca atctctttta aggtttcaat
     8701 ggcctctgcg gcctgttctt cagtaattgg tggacaaagg tcttttgcga tcttttttat
     8761 gttcacaaca ccaccattta agtctaaata agctcgaact gctggaatcc accagttttc
     8821 gagaaatttc aactcggctt gttgcaaact atgacgctct acatctctaa gagccacagc
     8881 tttagccatt aattccgctt ttttttctgc acttttagaa attcctgctg ccactaataa
     8941 atcaaaatac tcagcagaac gtccagatac atcgagtagt tcttttgccg caggaataac
     9001 atgtgctggc aaatgttgct ttttctgaag cactcgatac aggtagcttg agtctagacc
     9061 caatttatca cccatcattc tataggaata gaatggcatc tcttttttac gttgttcgta
     9121 ataagacttt aagagatcgc gataatccgc tacgtctgaa aaatttgtca tgttacatcc
     9181 aatgctaaaa aatatagccc agtgttgatg taaaaaaaaa tatacctttc ctattcgaaa
     9241 gaggctcatt gtgacaaatc tgtatggata gattgtccat gtgctttttt cattaaaaaa
     9301 ggacgctaca tttacacatc aatccatctc ataatagtaa caaaaaaata cgattaaacg
     9361 attctgaaaa ctgcactttt aacactaaaa aaatcaagaa aaacgctaaa aagaagaaat
     9421 aggacaaaaa agtatcaata acttgttctt aaaacataag ccaacaatca ttaatcgtta
     9481 gaaaaaaata acaaaaaata cagtttatcc taaagaccaa tatatcaatt ctcccttgat
     9541 ttatatgggc tcaatgtgat ttacatcata ctccaattag caagtaaaat agataaatca
     9601 acgctattcg agcataaatg ctgttgataa catcaaaaaa gtatggtatt aatcgcttcc
     9661 cttattctta atgaaaacta attgcttcta atacggcaat gaattctatt ttggggttaa
     9721 aaagaagaaa gacaaggcta ttcctaaatg agatttaatc tgtggacaaa ttgtcaataa
     9781 atattttttt acagctaaaa aaaaaccata cccgaaggta tggctaatca gataaaaaag
     9841 ttaaatacta cttgattata accttagctg tggaggaaga cgattgttgt cgcaattgca
     9901 caaaagcgac tccacggaat cccgttttta aagaaaccgt attcaaacct ttctcaacac
     9961 taatgctttt ttctacaatc acttgcccta gtgaattcat tagaacaagt gtaccctttg
    10021 aacttctttc actttcaaaa gacacgttca attgatttgc attctgtgta aaacgtagct
    10081 tagtggaaag cgtctttatt ggcgaagcac aaattttgat aggtccactt attaccgttg
    10141 ccacaagttc tgtgttagat acatttcctt ctgttgtagg aagtttttta ttaaatgttt
    10201 ctaaagtatc ctttgtacca tcagccttcc ataaaaccac attatcaatt cgaatttcac
    10261 cttcaaaacc taacgaataa agatttatca ctaaagcatt tatactcgta ggattttcaa
    10321 tatacagtat ctgttgattt tttgcagctt gagactcatc agcaatattt ttagaaagag
    10381 tatacttaaa ttcagcccat tgacccatcg tgagttcttg agaatgtaca tcatcccatg
    10441 cccaatccgt accactcata acagcaagat caatagaagc aaaaccatag gcagtatcga
    10501 cttgatgagt cgttccgtct acatatccta agaaagatac cgctgtataa ttactccaat
    10561 ctttgattga aggagtaata cgaatggtcg ttgttgtaga agtatcaggg cttgcctttg
    10621 tttcaacaag aacagactta tttgatgtat cggtactaag attcacaaga gcgtctatgg
    10681 agtttccttg aagtgtatct aaaccatagg ctttaagcat ggaatttaac acttcgtaat
    10741 caaaaattga tccagtggtt cctttttgac gacgaatcac tgtcatattt ccttcgcctt
    10801 catctcctgt atcccaaagc acaggaatca ttccatgatt ctttgagttt ttagccacag
    10861 ctccataaaa agccgctcta gattgcaaat gaagttttag attatcccct gaaagagata
    10921 gacgtttcac agccccaaac tctcatataa caacaggcat ctgttttgca gcaaatttag
    10981 tacttacaga cgaaagtgtt tcatccaccc aagctgggct tgcaaaacca cttgcaggtg
    11041 tattatgtac tgcatctgta gaagagagat aattccccca ataatagtag cacttacccc
    11101 aatccgcatc ttctgtcatt aatg
//
LOCUS       DAOOBB010000049        11062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_7512_length_11062_cov_4.589080, whole genome shotgun sequence.
ACCESSION   DAOOBB010000049 DAOOBB010000000
VERSION     DAOOBB010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 11062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11062
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7512_length_11062_cov_4.589080"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(466..912)
                     /locus_tag="PLT31_03715"
     CDS             complement(466..912)
                     /locus_tag="PLT31_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94276.1"
                     /translation="MTFDFHTTDFVIAEIKEKHQQKLIAKYIENGKLFIKKFDSEETG
                     ELISFFSNYSNKISIQDCSVWHYAKKTNARLLTGDLRLRKMIEQENAVQVSGIIFVFD
                     EMIKQKIISKKYACTKLIELMGINPRLPNSICNEKIELWRSKDEND"
     gene            complement(991..2046)
                     /locus_tag="PLT31_03720"
     CDS             complement(991..2046)
                     /locus_tag="PLT31_03720"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HPW94277.1"
                     /translation="MNHLKNFGKRLKNARIMKGLSMDELCQKMDNSLSKMAISKYENG
                     KSMPNSTVLISLAKALNVSVEYFFRPSVFSVESIHFRKKASLPQKKISSLKEVISDFA
                     ERYIEIEDICAVENPFAYSDYKGIKQKEDVLNAALKIRQSWNLGQDGIVNIIELLEEH
                     GIKVLEIEAPAKFDGLSTIINEKHPIIVLNKDFSSERKRFTAFHELGHIVLQFDDSIE
                     EKEQESLCNAFANEMLIPQSAFVKQLGSSRHDISYVELQSMQKQFGISCDALMFKAKD
                     LGVISEQRYQYYCIKKNKNRKFKELVESSHYPMESSMRFERLVFRALSDALISYSKAS
                     SLLGKSLEMIRRKAALV"
     gene            complement(2223..2537)
                     /locus_tag="PLT31_03725"
     CDS             complement(2223..2537)
                     /locus_tag="PLT31_03725"
                     /inference="COORDINATES: protein motif:HMM:NF027067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPW94278.1"
                     /translation="MKNKMKEALSDVLQGTVDAGLKTSFTERELKEYGIVIKKIDFGP
                     AKIKKVRKEARLSQAVFAKVLNVSISSVRQWEQGTRKPTGSTKVLLNLLSKKPDLVDQ
                     LI"
     gene            complement(2534..2905)
                     /locus_tag="PLT31_03730"
     CDS             complement(2534..2905)
                     /locus_tag="PLT31_03730"
                     /inference="COORDINATES: protein motif:HMM:NF018050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPW94279.1"
                     /translation="MNAFESRWFKRWSKKNGLKKADLLDALERTINGSGVVNLGGNIY
                     KIRVAKNGQGRSGGFRTILIFKKGKRSLFIYGFEKNDQDNIDKGTLADYKKFAVTFLN
                     YTDDDINRLVNNGTISLLEEK"
     gene            complement(3076..>3536)
                     /locus_tag="PLT31_03735"
     CDS             complement(3076..>3536)
                     /locus_tag="PLT31_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPW94280.1"
                     /translation="VSGGYTSLQRHARAMGFSVLQVPLAPIKAKSIQKGLPQEASIAS
                     NASLQQDGSTANRSSLHQTEHSAADTNTPIIVDVRTSREFARGAYPNAINIPLDELEE
                     RIDEFGPLTRKITVYCASGARSEYAKEILERNGFTNVINGGGIIQMMHRK"
     assembly_gap    3537..3636
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3637..5288)
                     /locus_tag="PLT31_03740"
     CDS             complement(<3637..5288)
                     /locus_tag="PLT31_03740"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPW94281.1"
                     /translation="MEKILVIGGVAAGATAAAKLRRLSGDVEITVLEAGPDISFANCG
                     LPYYIGRDIENRSSLILQSPVSFKEQYNVNVFTNTKAIKIDREQKLVYTENTKTNEKK
                     TFEYSKLILAQGGLPLTPPIEGASLPHVFTLWTLANMDAIDAFIKEQKPKKATIVGGG
                     FIGLEMAEALAKRGLKVSVVEKMPHVMNVVDPEFAGFIEEELLSYGLEVYKNVGVQVI
                     GKDQVLLDNGSAIESDMVLLSIGVRPTLDLAKDAGLEIGSTGGLLVDADLRTSDPSIF
                     AAGDMIEIEHRVHQAKVRIPLAGPANKQGRIAAENALGGNHLYPGSLGTSIVRVFEAV
                     VGITGLSLKAAKAAGIDAAAVTVHKEHHVAYYPGAQEVTVRLIYDKTTGCVIGGETAG
                     YAGADRRLDVIATAVFGRMTIHDLANIDFAYSPPLGTANDAINMAAFSAENRNSGYAP
                     SLTPEELEEWIKKEKPLILDVRDPFVYNAGHVENAHYLPPEILELEMAKIPKGHSVLV
                     YDENGKKGHQMLRKIQGLCPCNVVNVSGGYTSLQRHARAMGFS"
     gene            5502..5574
                     /locus_tag="PLT31_03745"
     tRNA            5502..5574
                     /locus_tag="PLT31_03745"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5535..5537,aa:Ala,seq:cgc)
     gene            complement(5627..6628)
                     /locus_tag="PLT31_03750"
     CDS             complement(5627..6628)
                     /locus_tag="PLT31_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94282.1"
                     /translation="MKMSLKNYRKELRDSLLDLAWVQWSALGVAGHVTRFSSYVVDVE
                     ALVLFTIFIGRYDERLFDSMQDWLQTNGRFVNTQRLKVLAKKNGFRYQRELAYVAANQ
                     NAFENKSKWKNIADSVKITSGDSVEPLFKDIPLLGKQLKDPLALKFGLERNIYKYAGK
                     NAKFDDGLASLMLRLRGLFGVNSRVEIMLLLLSNDFCKIQDLAKRSGYSWRSVQDILF
                     EMTFGDIVQATASKKNRVYSLPNKSDWLKTFGFQDDEPIRFPQWDKALFSLELIYETL
                     ENPLLDKLSNLSVQGAVNECFKKIGTSFLESSIRELQTVSSNSWLEIPEMMSRIFKK"
     gene            complement(6603..7193)
                     /locus_tag="PLT31_03755"
     CDS             complement(6603..7193)
                     /locus_tag="PLT31_03755"
                     /inference="COORDINATES: protein motif:HMM:NF039330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HPW94283.1"
                     /translation="MKEKTIDISKLNETLTLLNEQLLLQKSEPVHLVVCGGSALIATG
                     LVPRTTQDVDVVAMMEGDILKDPEPLPDKLINAAQATANLLKLPANWLNCGPADLFRM
                     GLPEGFVERLQTKVIGDCLVIHYVSRTDQIHFKLYASVDRGGYHVTDLRALNPTADEL
                     FMAAKWCTTQDVSEPFLYLLKEFLKAFEYENVAEKL"
     gene            complement(7394..8017)
                     /locus_tag="PLT31_03760"
     CDS             complement(7394..8017)
                     /locus_tag="PLT31_03760"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HPW94284.1"
                     /translation="MSAALVLSFIDQLKVINLSSLIPLGVGAGAYFIFLFVLSLLNRS
                     IEFAQTFCHELNHTFFSLLFFKKIKGFRAHADNGGEVSFEGKTNIMITLAPYSVPLFA
                     VLTLLLLLLAEPRVVPAIHFVVGFFISFHLHSVFSEFRLYQSDLRVYGIFLSFAFVVF
                     VNLLFLLWIISVSADDFSAGFHFLKNAFFSLRDVGMFLVDKAMAVVK"
     gene            complement(8062..8268)
                     /locus_tag="PLT31_03765"
     CDS             complement(8062..8268)
                     /locus_tag="PLT31_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94285.1"
                     /translation="MKKYILMILMVLGFVACSDPAKEAEVKTLENKVTDLQNVISKTK
                     DSISALEEKERALKKELESLDMVK"
     gene            8342..10780
                     /locus_tag="PLT31_03770"
     CDS             8342..10780
                     /locus_tag="PLT31_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HPW94286.1"
                     /translation="MNKSELLLPVGTKEMLAAAIHNGADAVYLGVPGWNARGRTEDFS
                     LDDLEEMITYAHLRDVRVFFAMNVLVFERELESLTPYIEQLVHLRPDAFIIQDVGLAR
                     LIHSIAPDQEIHASTQMTISSSEAIEMVSSLGFKRVVLARELSLLQIQRIKESTSIEL
                     EVFIHGALCISYSGQCLTSESFGGRSANRGQCAQSCRLPYKLFVDGKEQKQQGYQYLF
                     SPYDLCAIDQIKELIEIGVTSFKVEGRLKSPEYVAATAKAYRNQIDTGTITKEQKEAL
                     NILFSRGLSTGFLKGINHQTLVNGYFSNHHGEYLGTVIKADQSTVWIKSEVIPAPGDG
                     ILFEDEKNSNGSRLYNATKESRGIVRLEFSNQFKATDININSRTYRNDSPNLEKELRK
                     TFTDRQVEKRIPVQIKLNARVGTPLTLEMGTSAEMRVIVQGTLVEKAQKPTAPKIEKE
                     LKALSSTPYYAGTLSINVDADSFIQEKTIRVLKQEAVEKLNEKRLQEYKEPKVFQNAS
                     LIEEVKQKARAGAINTPQLSVLIRNPKQLDALENLPIQKVIMDFDWGVDYKPSLEKAK
                     TFGFKAGIATLRIHQPGENHYLKKIEECAPDFVLVRNLGALSILKKTNLPLFGDYSLN
                     VTNTLSADWFFEQGLSLIHPSWDLNSTQLFELLDTSNAPQFEIALHQYIPAFHTRHCV
                     FAALLTNGRQFPECQKICQAHQVEILDHKGERHFLQSDAECRNTLFVGKPQSALKLLR
                     TLREKGVYQFRLEMLDDSIENVRKKVALYADALFERISIEKAISLAGIEEKYGLSEGQ
                     LFNENTWINRKKKS"
     gene            10844..>11062
                     /locus_tag="PLT31_03775"
     CDS             10844..>11062
                     /locus_tag="PLT31_03775"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014544873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPW94287.1"
                     /translation="MKIATNSVVSMHYTLTSDEGSVIDTSAGREPLQYIQGKSMIVPG
                     LEKAMEGHTAGEKFDVKVIPAEGYGERNE"
BASE COUNT         3507 a         2363 c         1891 g         3201 t
ORIGIN      
        1 tacacgcccc aacaaactga aaataaaata aaatagtaac gtcaagaata atgaaataat
       61 caatccatac gaaacataac taacagtgaa aacactcttt cgaagcaccg ttgctataat
      121 tatacagatt tctgcaataa tgatgcataa tggcacaaac caaagtacca gccatacttt
      181 tttgaatcga aaatttttat aaaaaaaaga gaacagaaag cctaacatat acgggagcat
      241 attgaaaatc aaggacatga ttaaaaagaa aagaaaatta tctaaatcac taaatattgt
      301 cagaaaaaag tttatcattt aattatactc ctattcgtat agtaatattc ttttcttaaa
      361 ctatcttggt atggtgtgaa cgtatccata tttccgtgat tttttatttt gctcagtatg
      421 atcttatgaa tttaactttt ttaataggta aatagctttt tgattttaat cattttcatc
      481 ttttgaacgc caaagctcaa ttttttcatt gcagatacta ttcgggagcc gaggatttat
      541 acccatcaat tctattaatt ttgtacatgc gtacttttta gagattattt tctgcttaat
      601 catttcatca aatacaaaaa tgatgccact cacttgcact gcattttctt gttctatcat
      661 ttttctaagc cgtaaatctc ctgttaacaa acgagcattc gtcttttttg cataatgcca
      721 tacagaacaa tcttgtattg atattttatt agaataatta ctaaaaaaag aaatgagttc
      781 gcctgtttct tcactatcaa atttttttat aaagagcttt ccattctcaa tatatttcgc
      841 tatcaatttt tgttgatgtt tttcttttat ctctgcaata acaaaatcag tcgtatgaaa
      901 atcaaaagtc aatgaaaaaa aatgcagcga gcaaatctat cgataataaa tctaaaaata
      961 tatttgtgtc actcactaca tacagccgct tcataccaaa gccgctttcc ttcgtatcat
     1021 ttcaagtgac ttgcctaaaa gagaggaagc cttggaatag cttatcaatg catcacttag
     1081 agccctaaag acaagccttt caaagcgcat tgaagattcc attggatagt gagagctttc
     1141 tacaagttcc ttaaatttac gattcttatt tttctttatg caataatact gataacgctg
     1201 ttcactaata acacctaaat cttttgcctt aaacataagc gcatcacacg atattccaaa
     1261 ttgtttttgc atagattgca attcgacata tgaaatatca tggcgagatg aacccaattg
     1321 tttaacaaaa gcggattgtg gtataagcat ttcattagca aaagcattac acaaagattc
     1381 ttgttctttc tcttcaatag aatcatcaaa ctgcaaaaca atatgcccaa gttcatgaaa
     1441 agcagtaaaa cgttttcttt cggaagaaaa atctttatta agaacgataa tagggtgttt
     1501 ttcgttaata atagtactta aaccgtcaaa ctttgcagga gcttctattt ctaaaacctt
     1561 tataccatgt tcttctaaca attctataat attgacaatt ccatcttgcc ctaaattcca
     1621 gctttggcgt attttgagag ccgcattaag tacatcttct ttttgtttta ttcccttata
     1681 atcagaataa gcaaaaggat tttctacagc acatatatct tctatttcaa tgtacctttc
     1741 cgcgaaatct gaaatgactt ctttgaggct gctaatcttt ttctgaggta aactggcttt
     1801 ttttctaaaa tggattgatt cgacagaaaa tacagaaggt ctaaaaaaat actctacaga
     1861 gacattaaga gcttttgcta aagaaattaa aacggtgctg tttggcatgc tttttccgtt
     1921 ttcatatttt gatattgcca tttttgaaag agagttatcc attttttgac aaagctcatc
     1981 cattgaaagg cctttcataa tacgggcatt ctttaaccgt tttccaaagt ttttcaaatg
     2041 gttcatactg cctccttaca gtttacaaat ataataaaaa aaagtagatt tgtaaacagc
     2101 tattttggaa acaacaacac ctttctcaaa tacaaaaacg gttattgcgc gatttaacaa
     2161 aaccgcattt gctcggctac ttttaattga acagcctcaa aaactcgtgg agtcaaaaat
     2221 ttttatatca actgatcgac aagatctggc tttttggaga gaagattaag caacaccttt
     2281 gtcgatcccg ttggttttct tgtaccttgt tcccattggc gtacagatga tattgacaca
     2341 ttcaagactt tcgcaaaaac agcttggctt aatctggctt ctttccgtac ttttttaatc
     2401 ttagctgggc caaaatcaat ctttttaatg acgataccat attcttttaa ttctctttct
     2461 gtaaacgatg ttttaaggcc cgcatcaacc gtaccttgca gcacatcaga caacgcttct
     2521 ttcattttat ttttcatttt tcctccaata atgatattgt cccattgttc acaagtctgt
     2581 ttatatcatc atccgtataa ttcagaaatg ttactgcaaa ttttttataa tcagccaatg
     2641 ttcctttatc aatattatct tgatcatttt tttcaaaacc atagatgaaa agagatcgtt
     2701 tacctttttt gaatataaga attgttctga atccgccgct tcttccttga ccattctttg
     2761 ctactcttat cttgtatata tttccaccaa gattaacaac accagatcca ttgatcgttc
     2821 gttcaagagc atcgagcaag tcagctttct tcaacccatt cttttttgac catcttttaa
     2881 accacctaga ttcaaaagca ttcatactga aatatacctc attgtgatac agttgtcaag
     2941 ttcaatataa taacagcacc tttctcaaat acaaaaaaat ctggcgccag gaaataggcc
     3001 agattatttg tataaaaacg gttattgcgc gatttaacaa aaccgcattt gcttggtatt
     3061 atatcattat cctgcttact ttcgatgcat catttgaata atgccgccgc cgttgattac
     3121 gtttgtgaaa ccatttcgtt caagaatttc ttttgcgtat tcgcttcggg ctccagaggc
     3181 acaatagacg gtgatttttc gagtcaaagg tccaaattca tcaatccgtt cttctaactc
     3241 atctaacggg atattgattg cattaggata agcacctctt gcaaattccc gagaggttct
     3301 gacatcgacg attatcggtg tatttgtgtc cgcggcggaa tgctccgttt ggtgtaacga
     3361 cgaacgattc gcggtactac catcctgttg taacgacgca ttagacgcga tactcgcttc
     3421 ttgtggcaaa cctttttgta tgctttttgc ttttatagga gctaacggga cctgaagtac
     3481 agaaaagccc atggctcttg catgccgctg taaagaggta tagccgccac tcacatnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngaaa agcccatggc tcttgcatgc
     3661 cgctgtaaag aggtatagcc gccactcaca ttcactacgt tacaagggca taacccttga
     3721 attttgcgaa gcatttgatg gccctttttc ccgttttcat catagactaa aacggaatgc
     3781 cctttaggga ttttagccat ttctaattcc aaaatttcag gaggcaagta gtgcgcgttt
     3841 tcaacatggc ctgcattata aacaaaagga tctcgaacat ctaaaattaa aggcttttct
     3901 tttttaatcc attcttctaa ttcttcggga gttaaagaag gagcataacc agaatttcga
     3961 ttttctgcgc taaatgcagc catattaatc gcatcgtttg cagtgcctaa tggcggagaa
     4021 taggcaaaat caatatttgc taaatcgtgg atcgtcatgc ggccaaaaac agccgtagct
     4081 atcacatcta aacggcgatc agcgcctgca tagcctgcgg tttcgccacc aataacgcaa
     4141 cctgtcgttt tatcgtagat taaacgcaca gtcacctctt gtgcgcctgg gtagtaagcg
     4201 acatgatgtt ctttatggac ggtaacagca gcggcatcaa tgccagccgc tttcgccgcc
     4261 ttcaaagata agcccgtgat ccccacaacc gcttcaaaaa ctcgcacaat agatgtacct
     4321 aaagagcctg gataaaggtg gtttccgcct aaggcatttt ctgcagcgat tcgcccttgt
     4381 ttatttgctg ggcccgccag aggaatacgc actttagctt gatgcactcg atgttcaatc
     4441 tcaatcatat cgcctgcggc aaaaatagaa gggtcgcttg tgcgcaaatc agcatcgact
     4501 agcaagccgc ctgtagagcc aatctcaaga ccagcgtctt tcgctaaatc aagtgtaggg
     4561 cgaacgccta tcgacaagag aaccatatca gattctatag cagagccgtt gtctaaaagc
     4621 acttgatctt tgccaatcac ttgaacgccc acattcttat aaacctctag gccataagac
     4681 aagagttctt cttctataaa cccagcaaac tctggatcga caacattcat cacatgcggc
     4741 attttctcca caacgctgac ttttaagccc cgttttgcaa gggcttctgc catttcaagc
     4801 ccaataaaac cgccaccgac aatcgttgcc ttttttggtt tttgttcttt gataaatgca
     4861 tcaatcgcat ccatattggc taaagtccag agagtaaaaa catgaggcaa agacgcccct
     4921 tcaatcggag gtgttagagg gagaccccct tgagctaaaa tcagtttgga atattcaaaa
     4981 gttttctttt cgtttgtttt ggtgttttct gtataaacaa gcttttgctc gcggtcgatt
     5041 ttgatcgctt ttgtatttgt aaaaacattc acattatact gttctttaaa actaactggg
     5101 ctttgtaaaa tcaaggaaga cctgttttca atatcccttc cgatataata aggaagcccg
     5161 caattagcaa acgagatgtc tggcccagcc tctaaaacgg tgatttcaac atctcctgac
     5221 aaacgacgta attttgctgc tgctgtagca cctgccgcta ctccaccaat aacaagaatt
     5281 ttttccataa atattccttt ttaataaaag cacccttaaa aaacaattat taatgacgcc
     5341 cttatgataa aataatcatc ttctaaaagg aatatataaa gatggcaatt aaaatatcag
     5401 tgactttatc acaaaagcga ctttcccctc tgaaaccatt atcttagcgt caaaatctct
     5461 tgccaagtta tatttcttaa aatatatttt gcctaatctt tggggatata gctcagttgg
     5521 tagagcgatg cgttcgcaat gcataggcca ggggttcgaa tccccttatc tccataagta
     5581 aaattagaaa aaccgtctct ctcgtatcaa atccgaagag gcggttttat tttttaaaaa
     5641 tacgggacat catctccgga atttctagcc agctattgga ggaaactgtt tgtaattccc
     5701 taatactgga ttccaggaaa ctagttccta ttttcttgaa gcattcatta actgcacctt
     5761 gtacggacaa attggataat ttgtccaata agggattctc cagtgtttca taaatcagtt
     5821 caagactaaa tagagctttg tcccactgcg ggaatctgat gggctcatca tcttgaaagc
     5881 caaaggtttt cagccaatcg ctcttgttcg gtaagctata aactcgattc tttttggatg
     5941 ccgtcgcttg cacaatatcg ccaaaagtca tttcaaagag aatgtcctga acgctacgcc
     6001 atgaataacc acttcgtttg gctagatcct ggattttaca aaagtcatta gaaagaagca
     6061 gaagcataat ttcaactctg gaatttacgc caaacaaacc tcgcagcctt aacatcaacg
     6121 aagcaaggcc atcatcgaat ttagcgttct ttccagcata cttatatatg ttgcgttcca
     6181 gcccgaactt caacgctaat gggtctttca attgttttcc caaaagcgga atatccttga
     6241 acaaaggttc aaccgaatcg ccagatgtga tttttacaga gtctgcgata tttttccact
     6301 tgcttttatt ttcgaatgcg ttttgatttg ccgccacata cgccaattcc ctttgatagc
     6361 gaaacccatt ctttttcgct aggactttca gtcgctgcgt gtttacaaac cgtccattcg
     6421 tctgcaacca atcctgcata gaatcaaaaa gacgctcgtc gtaacgacct atgaaaattg
     6481 taaaaagaac caaagcctct acgtctacca cataagagga aaatcgtgta acgtgccctg
     6541 ctacacccaa agcagaccac tgaacccatg caaggtctaa tagagaatcc cttagttctt
     6601 tcctataatt tttcagcgac attttcatat tcaaacgcct tcaaaaattc tttgagcaaa
     6661 tacaaaaagg gttccgagac atcctgcgtg gtgcaccatt ttgctgccat gaacaattcg
     6721 tcggctgtag gattcaatgc cctcaaatca gttacatggt agccgcctcg gtcaacggaa
     6781 gcatagagtt tgaaatgaat ctggtccgtt cgggaaacat aatggataac cagacaatct
     6841 ccaataacct tcgtctggag cctttcgaca aaaccttctg gaagccccat gcgaaaaaga
     6901 tccgctggtc cacagttcaa ccaatttgca ggaagtttca aaagattcgc agtggcctgt
     6961 gctgcattga tcaatttatc ggggagaggt tcaggatctt tcaatatgtc accttccatc
     7021 atggcgacta cgtctacatc ctgcgtggtt ctgggaacaa ggccagtggc aatgagtgca
     7081 gaccctccgc ataccaccaa gtgtacgggt tctgatttct gcaaaaggag ctgctcattc
     7141 agcagagtta atgtttcatt taactttgaa atatcgatcg ttttctcttt catattaaga
     7201 atatacatta ttttttagtg gttatactta ataatagaca aaaaaaggta aaatttttca
     7261 tttaaaagct gttcagatag cggaaaacga cagtttataa tacccgtaaa cgaaagtgct
     7321 tttattgcaa catttcaatt acgaaacgta ctatttcaaa taaaacgtaa tatttcagct
     7381 ggcgaaaaag actttatttc accaccgcca ttgctttgtc tactaaaaac atcccgacgt
     7441 cacgcaaaga gaagaaggcg ttttttaaaa agtggaagcc tgcggaaaag tcatcggcag
     7501 atacgcttat tatccacaat aagaatagca agttgacaaa gacgacaaac gcaaaagaca
     7561 agaaaatgcc atacacgcgc aagtcgcttt ggtacaagcg aaattcgctg aacaccgaat
     7621 gcaaatggaa tgaaataaaa aagcccacaa caaaatgaat cgcagggact acgcgaggct
     7681 ctgcaagcaa taagagcaac aaggttaaca ccgcaaacaa tggcacgctg taaggggcga
     7741 gcgtgatcat gatattcgtt ttgccttcaa aagaaacttc cccgccatta tcggcatgag
     7801 ctcgaaaccc ctttatcttt ttaaaaaaca agagactaaa aaaagtgtgg tttaattcgt
     7861 ggcaaaacgt ttgagcaaac tcgatgcttc gattcaaaag agaaagtaca aatagaaaaa
     7921 taaaataggc cccagcacca accccaagag ggatcaaaga agacaagttt attactttca
     7981 attgatcaat aaaagataat accaatgcgg cgctcattac ccaaaacagc aaggcaaacg
     8041 gaacgcgtag cagaaaacgc attacttcac catatctaaa gattctaatt cttttttaag
     8101 agctcgttct ttttcttcca aagccgaaat gctgtctttg gttttagaaa tcacgttttg
     8161 taagtctgtg actttatttt cgagggtctt cacttctgct tctttggcag ggtcagaaca
     8221 agccacaaaa cctaagacca ttaaaatcat taaaatatac ttcttcattt tttaaatata
     8281 taaaactcat acaatactat tcttatattt ttatcagcat ccttaaaaat tgagccttgc
     8341 cttgaataaa tcagaactac tacttcctgt tggaaccaaa gaaatgcttg cagccgccat
     8401 ccataatgga gccgacgccg tttatcttgg agtccctgga tggaacgcga gaggcagaac
     8461 agaagacttt tcgttagatg atcttgaaga aatgatcact tatgctcatt taagagacgt
     8521 cagagtattc tttgccatga acgttttagt ttttgaacga gaattagagt ctttgacccc
     8581 ttatattgag caattagtcc acttaagacc agatgccttt attattcaag atgtgggcct
     8641 tgctcgttta atacatagca ttgctccaga tcaagaaatc catgcctcta cacagatgac
     8701 aatctctagt agcgaagcga ttgagatggt ttcttcgtta ggatttaagc gagtcgtttt
     8761 agcccgtgaa ttatcactcc ttcaaataca gcgaatcaaa gaaagcactt ctatagaact
     8821 cgaagtcttt attcatggag ccttgtgtat ttcttacagt ggccaatgcc ttacgagcga
     8881 atcgtttgga ggccgctccg caaacagagg ccaatgcgcc caaagttgca gactccccta
     8941 caaactcttt gtggatggca aagaacaaaa acaacaaggc taccaatatc tttttagccc
     9001 ctatgacctt tgcgccatag accaaatcaa agaactaata gaaattggag tcacctcttt
     9061 taaagtcgaa ggccgcttaa aaagcccaga gtatgtggca gctaccgcaa aagcttatag
     9121 aaaccaaatc gacacaggca ctattaccaa agaacaaaaa gaagccttaa atattttatt
     9181 ttctcgtggc ctctccactg gatttctaaa aggcattaac caccaaaccc ttgtgaatgg
     9241 gtatttctcg aaccatcatg gcgaatactt aggcactgtt atcaaagcag accaatcgac
     9301 cgtatggatc aaaagcgaag taatccctgc tcctggagac gggatccttt ttgaagacga
     9361 gaagaattcg aatggaagcc gcttgtataa tgcgacaaaa gaatctcgtg ggattgtacg
     9421 tttagaattt tctaaccaat ttaaagccac agacatcaat atcaatagcc gcacgtatcg
     9481 caatgactcc ccgaacttag aaaaagaact ccgcaaaaca tttacagacc gccaagtaga
     9541 aaaaagaatc cctgttcaga taaagttaaa tgctcgtgta ggcacaccac taacactaga
     9601 aatgggcacc agtgcggaga tgcgtgtaat agtgcaggga acgcttgtag aaaaagcaca
     9661 aaagcctact gcacctaaaa tagagaaaga attaaaagcg ctgtcatcga ccccctatta
     9721 tgcaggaaca ttaagtatta atgtggatgc cgattctttt atacaagaaa aaaccattcg
     9781 agtacttaaa caagaagccg ttgaaaagct aaacgaaaaa agactacaag aatataaaga
     9841 accaaaagta tttcaaaatg cttctttaat tgaagaagta aaacaaaagg cgcgtgcagg
     9901 ggcaataaac accccacaat tatccgtatt aatacgaaat ccaaaacaac tagacgcttt
     9961 agaaaactta cccattcaaa aagtcatcat ggattttgat tggggcgtcg attacaaacc
    10021 ctctttagaa aaagccaaaa cgtttggatt caaggcaggg atagcaaccc ttagaatcca
    10081 ccaaccaggc gaaaatcatt atctcaaaaa aattgaagag tgtgcgccag actttgtctt
    10141 agtcaggaat ctcggggctt tatcgatcct taaaaaaacg aacttgcccc tatttggcga
    10201 ctacagctta aacgtcacca acaccttaag cgccgactgg ttctttgaac aaggcctctc
    10261 cttaatacac ccctcatggg atctcaacag cacacaactc tttgaattat tagacacttc
    10321 gaacgcacca caatttgaaa tcgccttaca ccaatacata cccgctttcc atacaagaca
    10381 ctgcgtattt gcagcactcc tcacaaacgg aagacaattc ccagaatgcc aaaaaatatg
    10441 ccaagcccat caagtcgaaa tcttagacca taaaggcgaa cgacactttc tacaatctga
    10501 tgccgaatgc cgaaacacct tatttgttgg caaaccccaa tcggccctaa aattactccg
    10561 aacattacga gaaaaaggag tctatcagtt tagactagaa atgcttgacg atagtattga
    10621 aaatgtaaga aaaaaagtag ccctttatgc agatgcctta tttgaacgaa tttcaataga
    10681 aaaggccata tcacttgctg gaatcgaaga aaaatacgga ctttccgagg gccaactatt
    10741 taatgaaaat acgtggataa atcgaaaaaa gaaatcttaa cactccttaa cagaactctt
    10801 ttttgtatct ttggctttta ctcattaccc ttaaggatat ttcatgaaaa ttgcaaccaa
    10861 ctccgtagtc tccatgcact acaccctcac ttctgatgaa ggaagcgtaa tagacacctc
    10921 tgcaggtcgc gaaccattac agtacatcca aggcaaaagc atgattgtcc ctggattaga
    10981 aaaagccatg gaaggccaca ccgctggtga aaaatttgac gtgaaagtaa tccctgccga
    11041 aggctatgga gaacgcaacg aa
//
LOCUS       DAOOBB010000050        10987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_7566_length_10987_cov_4.328851, whole genome shotgun sequence.
ACCESSION   DAOOBB010000050 DAOOBB010000000
VERSION     DAOOBB010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 10987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10987
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7566_length_10987_cov_4.328851"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PLT31_03780"
     CDS             complement(<1..257)
                     /locus_tag="PLT31_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HPW94288.1"
                     /translation="MNRNSKANIGELLLRQGIIDEDQLKHAMSEQERTGIMLSKILVR
                     LGMVSEETLTNILGVQMQSTTKMRIGEMLMAQGYITEAQLD"
     gene            complement(254..1252)
                     /gene="thiL"
                     /locus_tag="PLT31_03785"
     CDS             complement(254..1252)
                     /gene="thiL"
                     /locus_tag="PLT31_03785"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HPW94289.1"
                     /translation="MEENDYAFPDLGEFRLIQSLLKDRFPLSNPSPKHRFWVDAGDDA
                     AGVDGWLITKDLSMENTHFLLDYSTMAQAVEKHIVSNVSDISSMGGVPMIAFLGLGLN
                     RHWNEDQRNALIDAFQKGFKSRGIRLLGGDTVVGDVGFFSTTLLGQTTLVQPLKRSSA
                     KPGERVYVQGTLGKSAAGLWVLLNHPEDRKKWSSLVEYHLSPVIKERAGEALAEIQSI
                     GACIDISDGLSSELNHIASSSRVQLVIEKESLPVDPEVLALSEYYGLSPYDFALNGGE
                     EYQLLFTSSCSNSIFYNNDRIGSVYDIGYVQVGSGVEQINSQGIVEQMNAQSWSHL"
     gene            1335..1769
                     /locus_tag="PLT31_03790"
     CDS             1335..1769
                     /locus_tag="PLT31_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94290.1"
                     /translation="MGHIFFITLDSSCNLDEVAQWTLFWLLDHDMVANTTLYTCSGLE
                     QAYRALEKPLKTGTSPALIVIDYPMQPNAEMLQFVNRLRECIPETWIIEFIPRTMPMP
                     SEKEGHLFWIPKPVCQEDWTDVLKHVCFQAASPQWSEAENPY"
     gene            1777..2760
                     /locus_tag="PLT31_03795"
     CDS             1777..2760
                     /locus_tag="PLT31_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94291.1"
                     /translation="MKKGISYFGVRRPKWALEDMKEIKERGFTHVLHTWSEEDLLYYR
                     DSMQEIIDGSDELGLKVYVNPWGVGRVFGGEAFSELVGRNFSLAQKTVDGKSKVAACP
                     NQQEFRDYMDQWIQAVCSTKMETIFWDEPHFYFEKGNLNNWACCCDQCQKIFFDKYGF
                     KMPDSLTPEVQSFREDSLIDFLNVMTEKVHCLGKRNSVCLLPPWFPAGIENWDRIAEL
                     PFVDEISSDPYWEKTTSSEIIKENYREVSKQVLSVAHKYGKEAQIWIKNYHIVKDKES
                     DVRLATEEAYDAGIRNIFAWSYLGSEYLSWLRSDNPELVYETQISALNKLK"
     gene            complement(2779..4332)
                     /locus_tag="PLT31_03800"
     CDS             complement(2779..4332)
                     /locus_tag="PLT31_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HPW94292.1"
                     /translation="MNSMTRMVMVLLLMVATLWSAPAEGSVAPNKKLYDFSFVDIEFD
                     AAFRSLSIAAGVDILLSPDVKGKLNLKVSKKTWQETLDIICELNDLVWLVQDKYISIQ
                     RLASYQAKQKKIAEQEEQSQEVAPLIRKNFAIRHAKAQDLVSVLETMKSKRGKITVVE
                     RTNSIIVYDTDASIVQMKNALDELDVETLQIMITAKLVVVNSNLARELGVDWTSAMGS
                     TALTPGVASAPNGSNAGGTRTSAAIQSFPNNGVSGLPGAATSLTASILDNNLQIAINS
                     IMGDASTEILASPQVSTLDNTEAIVFMGDKVSIRVIDDAGESSTQLVETGIKLTVTPH
                     VSGDNRILLDLHPENNSYDYDTKGEIVISTQEAKTKVVVADGETVVIGGLTRNEDVES
                     ESGIPFLKDIPILGNLFKYTKKSVTKKDLVIFVTPRIIRNYIGNVQTSEPSEEKVQRL
                     VDNQPAPVTSPAKEESNKVPVQQEQPATQQAPAVSEPPPAPAAPVNDAQQNEAPQNDE
                     ATAEEGEDW"
     gene            complement(4329..6059)
                     /locus_tag="PLT31_03805"
     CDS             complement(4329..6059)
                     /locus_tag="PLT31_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94293.1"
                     /translation="MKKIKALLLIFFVVSTSFAADVKDVSMQCQKEACQLVFQFVSSK
                     NLPSFFQKYDPKSKILTVAFSETNFLLGDGTFDLNPTSPLIQKMKVFKETNKKVSLLK
                     FEFNVGDLIKTDKNKIELVKETHFMIVLPKANDKGWVLSKKFKELKKAAEKATLEEKK
                     RLAEEKKAAEKAALEEKKRLAEEKKAADKAALEEKKRKAAEKKTAGKATVVETKNVAE
                     SKPVSALIDGVQEMIALSSFGLEQFQLVTDDKIILTNVGPLNKQVITIGLAGPSKSPV
                     FKISRSSIVKSIYWSSFGLNLELQPGIEPSVLVRNGILILQKPSDKKIEGMWYWHAKP
                     TGIHERQWISTKSTSSSFDSFAKRLEPESKKIVSVAQTFYLRPTARELIVVSEETELY
                     AAASEKSQVLQRVVFGERLVSLENTGLFQKVQYNNKTGFVYKRAVSFRDELSSIQLER
                     LKQMAFEKGENLDSIASRFEKITEDRVTYSSFGRRDPFVEIKGLVEEGINIDQVELVG
                     IIWETEEPMAILAESKNPSISYTVKEGDKILNGKVLKITSTDVLFLIQEFGVSRRYSM
                     GLPDKYGSLK"
     gene            complement(6056..6655)
                     /gene="pilO"
                     /locus_tag="PLT31_03810"
     CDS             complement(6056..6655)
                     /gene="pilO"
                     /locus_tag="PLT31_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HPW94294.1"
                     /translation="MGNFDWKDKKNIFAILVVLLIGGVSFAFYQYKWTTFETKKQELE
                     QQLTSARDDLAKINVQKNRIAELSLRLAQAENEFERLKEMFPEEEKVPLRLQDLYAVL
                     RSSGVQIQKFNPEGREPKDYYIENKYSIVVNSGYHMLGYLFAEIANFNYPTSITDLRL
                     GRFSGIATELEKAETHGWTPITVSVSFKLTTYTSKKVAQ"
     gene            complement(6658..7260)
                     /locus_tag="PLT31_03815"
     CDS             complement(6658..7260)
                     /locus_tag="PLT31_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HPW94295.1"
                     /translation="MAEKKNKLCVEINLLPAEYRKVKNDLSWILDRRIVWPIVALLVV
                     AVLIFLLILHIEDVTSSLENELQTTLAEVEKEKPLLNKITELDNKLSVIAQKNNALKS
                     IQVSKKRWVILFENMSSILPPNMWLTNLNQIGANEMELKGFTYDFSEVAEYMVKLEKQ
                     VSISSVNLISIATTKTGGEDTYNFTIKAAIKQDLGLEGAK"
     gene            complement(7260..8348)
                     /gene="pilM"
                     /locus_tag="PLT31_03820"
     CDS             complement(7260..8348)
                     /gene="pilM"
                     /locus_tag="PLT31_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HPW94296.1"
                     /translation="MASDFISHMLGDRQTVGIDIGHYSIKLVRVFHSHRGNIQVTAVD
                     LERIPEKTLEDGQIKDSEKLREALSKIMARQVSDREKVDIVAGINSASGVLVDKLTVK
                     VPKNANEDAIIVQTAQARPPFDDQDNILDYQVYDRQDGEIKVNIVAAKNALLDSWAEF
                     YKDSDVRLNAIDVDVFALANVYMMSAEEEEKKETVAIFDIGEKKASLVFLMGGFFHST
                     RALTSGSMESVINMLCRHLGIDVAKCHEIFESDKPVMIDGFSEADIESAMQLAFEEVV
                     GALEFGIRYFSNSEAGEKPSKILLSGGGVSLSGLCEYIEERIGIHTEALSPFEKVSCA
                     PEVVGESGLSKALVNIYAPALGYAMRKF"
     gene            complement(8469..9482)
                     /locus_tag="PLT31_03825"
     CDS             complement(8469..9482)
                     /locus_tag="PLT31_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilZ"
                     /protein_id="HPW94297.1"
                     /translation="MDSGLIMIEPFVLIWIAVIVILLLLLLVLIEIQRSEKRRENEEM
                     FGTQSFERKKLLFGLTEKETHTIEKLVRQSTFSNKDAVFNSSSLFEEAVNRFYNFRKI
                     NQIRTETLASVSSLREKLGFTVNHPHVLLTSSRQFVVGIKVTLLSSLKTNKEHTVVTS
                     VDEKSWKVRVSGVWEEEFYHTLEGSFILMRWTQPEEAVYSGKVKVLKVTGNELELSHL
                     IEFEKLQLRRWIREQVLFPVQAFLSSSSKLEGFLFDLSAGGILIGLPVSEIPTHEIEI
                     QFRLPGFGLERVKIEILRNLGKKRQEYPDLYLYTASFSGEFGRIQEKVLQYIFQVHKK
                     KKT"
     gene            complement(9467..10714)
                     /locus_tag="PLT31_03830"
     CDS             complement(9467..10714)
                     /locus_tag="PLT31_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HPW94298.1"
                     /translation="MNKVILKNVRPFIDGAFQTPTTMNLVEGRWVDESIEGATEIDAK
                     GALALPALFALGLDFQEPLRDDIYTFQNGYTAMRRGGFYGGIYESTANPIDNVQKLSA
                     IQQVFSKSGLHLSILGAFSKHHMCEQLSEMLELASCGVCGFGDGNHLNATTRFLRLAM
                     EYGAMTSKRFFFLPLDLSLRCAGCVHEGDVADVLGMKGIPRIAETIVVHTILETALFL
                     NVPIHLKQITCSESLNLIEQARARGLDVTCDVGLYHLLFDDQAIFNLDSHYYIIPPLR
                     TPKDKEALWDGLKRGVIDAISCNHTPVLRQEKEVNFEDAVAGAISLEVALPAIWSTLV
                     DHVGERRALDLLSFNPAKIASAMPQKIEKGTIVNLVLLDPKEQSQVTPEKLAGAVRNT
                     PLLNAQLPSAIKGSYIHGVWTQV"
     gene            complement(10707..>10987)
                     /locus_tag="PLT31_03835"
     CDS             complement(10707..>10987)
                     /locus_tag="PLT31_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HPW94299.1"
                     /translation="AVIALRLQKERMDDSLLPSMREYRNFFGITREVLEQASPSALIM
                     HPGPINRGVELDSEMADSDQSVILEQVTNGVAVRMAVLFLLSGGRASE"
BASE COUNT         3464 a         2277 c         1800 g         3446 t
ORIGIN      
        1 ttatccaact gagcttctgt aatataaccc tgcgccatga gcatttcacc aatgcgcatt
       61 ttagttgtgg attgcatttg cacgccaaga atatttgtca gagtctcttc actcaccata
      121 cctaaacgaa caagaatttt actcaacata attcctgtac gttcttgttc actcatcgca
      181 tgcttgagct gatcttcgtc tataattcct tgacgaagga gtaattcacc tatattagct
      241 tttgaatttc tattcataaa tgcgaccatg attgagcgtt catttgttct actatccctt
      301 gagaattaat ctgttcaacg ccacttccga cttgaacata accaatatca taaacagaac
      361 caatcctatc attattatag aatatactat ttgaacagga acttgtaaaa agaagttgat
      421 attcttcacc cccatttaat gcaaaatcat agggtgacaa accgtaatat tcagacaaag
      481 ccaaaacttc aggatccaca ggtaaacttt ctttttcaat tacgagttgc actcttgaag
      541 aagaggcgat atgatttaac tcagaactta atccatcgct tatgtcaatg caggccccta
      601 tagattgaat ctcggcaaga gcttctcctg cacgttcttt gatcacagga ctcaaatgat
      661 attctacaag agaagaccat ttttttcgat cctcaggatg atttaataat acccaaagcc
      721 ctgcagctga ttttccaaga gttccttgaa cataaacacg ttcaccaggc tttgcactag
      781 agcgttttaa aggctgaact aaggttgttt gtccaaggag agtcgtagaa aaaaaaccaa
      841 catctcccac cacagtgtct cctccaagaa gacggatacc gcgagattta aatcccttct
      901 gaaaagcatc tatcaaagca tttctttgat cttcattcca atgacgattc aatcctaaac
      961 ctaagaaagc gatcatcgga actcccccca tagaggaaat atcagagaca ttagaaacta
     1021 tatgcttttc taccgcttga gccatagtcg aataatctaa tagaaaatgc gtgttttcca
     1081 tagataaatc ttttgtaata agccaaccat ctacacctgc agcatcatcc ccagcatcta
     1141 cccaaaaacg atgtttagga gaaggattcg ataacgggaa tcgatccttt agtaagcttt
     1201 gaatcagcct aaattcacct aaatcaggaa aagcgtaatc attttcttcc ataagcgtaa
     1261 atttaatgaa aaaaaagact tgaacaactc cttctataca ctaaagagaa aaaaaaaact
     1321 aattttagga gacaatggga catatatttt ttattacgct cgattccagt tgcaatttag
     1381 atgaagttgc ccaatggact ttgttttggc ttttagacca tgatatggtg gcaaatacaa
     1441 ctctttatac ttgcagtggg ttagaacaag cataccgtgc ccttgaaaag ccattaaaaa
     1501 caggcacttc tcccgcttta atagtcatag actatccaat gcagccaaac gctgaaatgc
     1561 ttcaatttgt aaaccgttta cgagaatgta ttccagaaac ttggattata gaatttatcc
     1621 cacgtaccat gccaatgcct tcagaaaaag aaggccactt attctggata cctaagcctg
     1681 tttgccaaga agactggaca gatgtattaa aacacgtatg ttttcaagcc gcttcacctc
     1741 aatggtctga agcagaaaat ccatattaat aaaactatga aaaaaggtat tagttacttt
     1801 ggagtccgtc gtccgaaatg ggctcttgaa gacatgaaag aaattaaaga gcgagggttt
     1861 actcacgttc ttcatacatg gtcagaagaa gatcttttat attaccgtga ttccatgcaa
     1921 gaaattattg atggttccga tgaattaggg cttaaagttt atgtaaaccc ttggggcgtt
     1981 ggaagagttt ttggtggaga agccttttct gaacttgtcg gaagaaactt ttctttagct
     2041 caaaaaacgg tcgatggaaa atctaaagtt gccgcttgcc ctaaccagca ggaattcaga
     2101 gattatatgg atcaatggat tcaagccgtt tgttctacca aaatggaaac catcttctgg
     2161 gatgaacccc atttttattt tgaaaaaggc aatcttaata actgggcttg ttgttgtgac
     2221 caatgccaaa agattttttt tgacaaatat ggttttaaaa tgcccgattc tctaactccc
     2281 gaagtacaat catttagaga agattcatta attgactttt taaatgtaat gactgaaaaa
     2341 gtgcattgtt tagggaaacg aaattctgtt tgtcttttac ctccatggtt cccagcggga
     2401 atagaaaact gggatcgtat cgctgaatta ccttttgtag atgaaattag ttctgaccct
     2461 tattgggaaa agacaaccag ttctgaaata attaaagaaa attatagaga agtgtctaaa
     2521 caagttttat ccgttgctca taagtacgga aaagaagctc aaatttggat aaagaactat
     2581 cacatcgtca aagataaaga atccgacgta cgtctcgcta cagaagaggc atacgatgcg
     2641 gggattcgta atatttttgc ttggtcatat ctaggttcag aatacttatc atggctacgt
     2701 tctgataatc cagaactggt ctatgaaaca caaatcagcg cactcaataa gcttaagtag
     2761 acctattaat agatctactt accaatcttc accttcttca gctgtggctt catcattttg
     2821 aggagcctca ttttgctgag catcatttac tggagcagcg ggtgctggtg gaggttctga
     2881 tacagcagga gcctgttgtg tcgctggttg ttcttgctgt acaggaactt tatttgattc
     2941 ttcttttgca ggagaagtta caggagcggg ttgattatct accaaacgct gcactttttc
     3001 ttccgaaggt tctgatgttt gtacattacc gatataatta cgaataatac gaggggtaac
     3061 aaatatgacg aggtctttct ttgtcactga ttttttcgtg tatttgaata aatttcctaa
     3121 aatagggata tcctttaaga aaggaattcc actttcagac tccacatctt catttcttgt
     3181 taatccacca atcacgaccg tttcaccatc agctacaacc actttggtct tagcttcttg
     3241 agtgctgatc actatttcac ctttggtgtc ataatcataa gaattattct ctggatgtaa
     3301 atctaaaaga atacggttat ctccagaaac atgaggtgtg acagtcaact taataccagt
     3361 ttctacaagc tgtgttgaag attcgcctgc atcatcaatc acacgaatgg aaactttatc
     3421 acccataaaa acaatggcct cagtattatc taaagtagat acttgaggac ttgctaaaat
     3481 ttctgtagaa gcgtctccca taatgctatt aatagcaatt tgcaaattat tatctaaaat
     3541 acttgcagtt aaagaagtcg ctgctccagg taaaccagat actccgttat tagggaatga
     3601 ttgaatggca gcacttgttc ttgttccgcc ggcattactt ccatttggtg cagaagcgac
     3661 gcctggcgtt aacgccgttg agcccatcgc gcttgtccaa tcaacaccta attcacgagc
     3721 aagattacta ttgaccacca ctaattttgc cgtaatcatg atttggagag tttccacatc
     3781 caattcgtct aaagcatttt tcatttgaac aatacttgca tcggtatcat atacaataat
     3841 cgaattagtc ctttctacaa cggtaatttt gcctcttttc gatttcattg tttctaaaac
     3901 acttactaaa tcttgggcct tggcatgacg aatagcgaag ttcttacgaa tcagaggagc
     3961 aacctcttga ctttgctctt cttgttcagc aatcttcttt tgctttgctt gataggaagc
     4021 aagcctttga atagagatat acttatcttg aactaaccat actaaatcat ttaattcaca
     4081 gataatatct aatgtttctt gccatgtctt cttagagact tttaaattca atttcccttt
     4141 gacatctgga gaaagtaaaa tatcaactcc agctgcaatg gaaagagatc taaatgcagc
     4201 gtcaaactca atatcgacaa atgagaagtc atatagcttc ttatttggag ctacggatcc
     4261 ctctgcagga gcagaccaaa gagtagctac cattaacagc aaaaccatta ccattcgagt
     4321 cattgaattc attttaaact cccatattta tcgggcaaac ccatggagta acgacgactg
     4381 actccaaatt cttggattaa gaacagtacg tctgttgatg taattttaag cacttttccg
     4441 ttcaagattt tatcaccttc tttaacggta taagaaatag atggattttt actttcagca
     4501 agaatagcca taggttcttc ggtttcccaa ataattccca ctaattccac ctgatcaata
     4561 ttaattcctt cttctactaa acctttaatt tcaacaaaag gatctctacg accaaaagaa
     4621 ctatatgtca ctcgatcttc tgtaattttt tcaaaccgag aagcaatcga atccagattt
     4681 tcaccttttt caaaagccat ctgttttaat cgttctaatt gaatgcttga taattcgtca
     4741 cggaaagaaa cggctctttt ataaacaaag cctgttttat tattatactg cactttctga
     4801 aacaaacctg tattttcaag acttactaat cgttcaccaa aaacaacacg ctgtaaaact
     4861 tgagattttt cacttgcagc cgcatacaat tctgtttctt cagaaacaac gattaattct
     4921 cttgcagtag ggcgaaggta aaaagtctga gctactgaaa ctattttttt agattcaggt
     4981 tctaatcttt ttgcaaacga atcaaaagaa gaggatgtcg atttggtgct aatccattga
     5041 cgttcatgaa tgcctgttgg ttttgcatgc caataccaca ttccctctat ctttttatca
     5101 gaaggcttct gtaaaatcaa aattccattt cgaacaagta ctgaaggctc aattcctggt
     5161 tgaagttcta agttcaaacc aaaagaactc caatatatag actttactat ggagcttcta
     5221 cttattttaa atacaggtga tttggaaggc ccagctaagc caatcgtaat cacttgttta
     5281 ttcaatggac caacatttgt taaaattatt ttatcatctg tcactaattg gaattgttct
     5341 aaaccaaaac tagacaaagc gatcatctct tgaacaccgt caattaaagc cgacacaggc
     5401 tttgattcag caacattttt agtctctacc actgttgctt ttcctgcagt ctttttttct
     5461 gctgctttac gtttcttttc ttcaagtgcc gctttatctg ctgccttctt ttcttctgct
     5521 aagcgttttt tttcttcaag agctgctttc tctgcggcct tcttttcttc tgctagacgt
     5581 ttcttttctt caagtgttgc tttctccgcg gccttcttta attccttaaa ctttttagat
     5641 aaaacccaac ccttatcatt tgcttttgga agaacaatca taaaatgagt ttctttaaca
     5701 agctctattt tatttttatc tgttttaatt aaatctccaa cattaaactc aaattttagc
     5761 aaggacactt ttttattggt ttccttaaat actttcattt tctgaattaa aggagaagta
     5821 ggattcaaat caaacgttcc atcgcctaaa agaaaatttg tctcagaaaa agcaaccgtt
     5881 aatatttttg attttggatc atacttttga aaaaaactag gcaagttttt ggaggaaaca
     5941 aattgaaaaa ctaattgaca agcttctttt tgacactgca tactaacatc tttaacatca
     6001 gcagcaaaag aagtcgacac tacaaaaaag ataagaagaa gggcttttat ttttttcatt
     6061 gcgcaacctt cttggatgtg taagtggtca atttaaatga gacagaaacg gtaattggag
     6121 tccatccatg agtctccgct ttttcaagct ctgtcgcaat gccagaaaaa cgtcctaaac
     6181 gcaaatccgt aattgaagta ggataattaa aatttgcaat ctcagcaaaa agatagccaa
     6241 gcatatggta accagaattc actacaatag aatacttgtt ttcaatatag tagtctttgg
     6301 gctcacgacc ttctggattg aatttttgaa tctgcacacc agaacttctt aacacggcat
     6361 ataaatcttg taaacgcaga ggtacttttt cttcttctgg gaacatctct ttaagacgtt
     6421 caaattcatt ctctgcttga gccaaacgaa gactcaactc tgcgattcta tttttttgaa
     6481 cattaatttt agctaagtca tcgcgagcgc tagttaactg ttgttctaat tcttgttttt
     6541 tagtttcaaa agtagtccat ttatattgat aaaaagcaaa actaacacca ccaatcaaaa
     6601 gcactactag aatagcaaaa atattttttt tgtctttcca atcaaaatta cccataacta
     6661 cttagctcct tccaggccaa gatcctgttt aatcgctgct ttaatagtaa aattataggt
     6721 atcttctccg cctgttttcg ttgtcgctat agagattaag ttgactgaac taatactaac
     6781 ctgtttttcg agtttgacca tgtattctgc aacttcagaa aagtcataag taaacccttt
     6841 taattccatt tcatttgcac caatctgatt taaattcgta agccacatat ttggaggcaa
     6901 aatggatgac atgttttcaa aaagaatgac ccatcttttt ttactgactt gaattgattt
     6961 aagtgcatta tttttttggg cgatcaccga aagtttatta tctagttcag tgattttatt
     7021 aagtaatggt ttttcttttt ctacttcagc aagagtcgtc tgcaactcat tttctaaaga
     7081 agaagtcaca tcttcaatat gaagtattaa taaaaagata agtacggcta cgactaacaa
     7141 ggcgacaatt ggccatacaa tacgacgatc taagatccag cttaaatcat ttttaacctt
     7201 cctatattca gcaggaagta aattgatttc aacacaaagt ttatttttct tttctgccat
     7261 tagaatttcc tcatggcata cccaagtgcg ggagcataaa tattaactaa cgctttagaa
     7321 agacctgatt caccaacgac ttctggtgca caagaaactt tttcgaaagg gctcaaggct
     7381 tctgtgtgaa ttccaattcg ttcttcgata tattcacata aaccagaaag actaacgcct
     7441 ccaccactca ataaaatttt acttggtttc tctccagctt ctgaattaga gaaatatcga
     7501 ataccaaatt ctaatgctcc aacgacttct tcaaaagcga gctgcatagc agactcaatg
     7561 tccgcctcag agaaaccatc gatcatgaca ggtttgtcac tttcaaatat ttcatgacac
     7621 tttgcaacgt ctataccaag atgacgacaa agcatattga tcacagactc cattgaacca
     7681 ctagtcaaag ctcgagtaga atgaaaaaag cctcccatta aaaagacaag gctagccttt
     7741 ttttcaccaa tatcaaaaat agccaccgtt tcttttttct cttcttcctc tgcagacatc
     7801 atataaacgt tcgctaaagc aaaaacatcc acatcaatgg cgtttaagcg aacatcggaa
     7861 tctttataaa actcagccca tgaatctaac agcgcatttt ttgcagccac aatattgact
     7921 ttaatttcac cgtcttggcg atcataaact tgataatcta atatgttatc ctggtcatca
     7981 aaaggaggac gagcttgtgc cgtctgaaca atgattgcat cttcatttgc attctttggc
     8041 actttaacgg ttagcttatc gacaagcact ccagatgcac tattaatccc agcgacgata
     8101 tccacctttt ctcgatcgct tacttgcctt gccataattt tagacaaagc ttctcttaat
     8161 ttttcagaat ccttgatttg cccatcttct aaagtctttt ctggtatacg ttccaaatca
     8221 acagcggtga cttgaatatt ccctcgatga ctatgaaata cacgaaccag cttgatactg
     8281 taatgaccaa tatcaattcc gaccgtttgt cgatcgccaa gcatatgtga aataaaatca
     8341 gaagccaaaa taaactccga cgggaaacat tatcatctca attctacctt tcgcaagtgc
     8401 gaatgtcaag caaaaaatac ataactcctt gaaaaataat tagttagacc tgtttttacc
     8461 acctataatt acgtcttttt ctttttatga acctgaaata tgtattgaag caccttttct
     8521 tggatacgac caaattcacc tgaaaaggaa gcggtgtata aatataaatc ggggtattct
     8581 tgcctttttt tgcctaaatt acgtaaaatt tcaattttta cacgttctaa tccaaagcca
     8641 ggtaatctaa actgtatttc tatttcatga gttggtattt cacttactgg taacccaatt
     8701 agaatacctc ctgcagataa atcaaacaaa aagccttcca acttagacga actcgataga
     8761 aatgcttgaa caggaaataa gacttgttct cgaatccaac gtcttaattg tagtttttca
     8821 aactcaatta aatgagaaag ttctaattca ttacctgtca ctttcaaaac ttttactttt
     8881 ccagagtaaa ctgcctcttc tggttgagtc caacgcatga gtataaaaga cccttctaac
     8941 gtatgataaa attcctcttc ccaaactcct gatactctta ccttccaact tttttcgtcc
     9001 acacttgtaa ctacagtatg ttctttgttt gttttcaagg aggaaagaag agtaacctta
     9061 atccccacta caaattgcct tgaactcgtt aaaagaacat gaggatgatt gacagtgaag
     9121 ccaagctttt ctcttaaaga agaaacggaa gctaatgttt ccgtacgaat ctgattgatt
     9181 ttacgaaagt tataaaatcg atttacagct tcttcaaata gactagagga attaaaaaca
     9241 gcatccttat tcgaaaaagt agactggcgg actaattttt ctattgtatg cgtttccttt
     9301 tctgttaaac cgaaaagtaa ttttttcctt tcaaagcttt gagtcccaaa catctcttcg
     9361 ttttcacgac gtttttcact tcgctgtatt tcgattaata ctaaaagtag tagtaataaa
     9421 ataactatta ctgcaatcca tattaagacg aagggctcaa tcataattag acctgagtcc
     9481 aaaccccatg aatataagag cctttaatag cactaggtaa ttgagcattc aatagaggtg
     9541 tatttcggac agctcctgca agtttctctg gcgtgacttg actctgttcc ttaggatcca
     9601 aaaggactaa attgactatc gttccctttt caatcttttg aggcatggct gaagcgattt
     9661 tcgctggatt gaatgaaagt aaatccagcg cccgtcgttc accgacgtgg tctactaatg
     9721 tagaccaaat cgctggtaaa gcaacttcta aagaaatagc gcccgcaact gcatcttcaa
     9781 aatttacttc tttctcttga cgaagaacgg gggtatgatt acagctaata gcatcaatca
     9841 cccctcgctt aagaccatcc caaagagctt ctttatcttt aggagtacga agaggtggaa
     9901 taatataata gtgagaatcc aaattgaaaa tagcttgatc atcaaatagt aaatgataaa
     9961 gccctacatc acaagtgacg tctagacctc gagctctcgc ttgttcaatt aggtttaaag
    10021 attctgaaca agtaatttgt tttaaatgaa ttggaacatt caagaaaaga gctgtttcta
    10081 aaatggtgtg cactacgata gtctcagcaa ttctaggaat acccttcatt ccgagaacat
    10141 cagccacatc cccttcatga acacatcctg cacagcgtaa agaaagatct agaggcaaaa
    10201 agaaaaaacg cttggatgtc atcgcaccgt attccatcgc taagcgtaaa aagcgagtcg
    10261 tagcatttaa atggtttcca tctccaaagc cacatactcc acacgaagca agctctaaca
    10321 tctcagatag ctgctcacac atatggtgtt tactaaaagc acctaaaatg gaaagatgta
    10381 atccactttt tgaaaatacc tgttgtatag cacttaactt ctgaacatta tcaatagggt
    10441 ttgcagtact ttcatatatt ccaccgtaaa atccgccacg acgcatggca gtataaccat
    10501 tttggaaagt gtatatatca tctcggagag gttcctgaaa atcaagcccc aaagcaaaaa
    10561 gagctggtag agcaagtgct cccttagcat ctatttcagt agcaccttca attgattcat
    10621 cgacccaacg gccttccaca agattcatgg tcgtaggtgt ttgaaaagct ccgtcaataa
    10681 aaggacgaac attttttaga atgaccttat tcactagcac gccctcccga aagaagaaat
    10741 aaaacagcca tacgtacggc gactccatta gtcacttgtt ctaaaataac agattgatca
    10801 ctatcggcca tttcagaatc aagttctacc cctcgattga ttggaccagg atgcataatt
    10861 aaagcagatg gacttgcttg ttcgagaact tctcgagtaa ttccaaaaaa gtttcgatac
    10921 tcacgcatgc ttggtaaaag cgaatcatcc atccgttctt tttgtaaacg taaggcaata
    10981 accgcat
//
LOCUS       DAOOBB010000051        10615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_7873_length_10615_cov_4.894697, whole genome shotgun sequence.
ACCESSION   DAOOBB010000051 DAOOBB010000000
VERSION     DAOOBB010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 10615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10615
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7873_length_10615_cov_4.894697"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="PLT31_03840"
     CDS             complement(<1..655)
                     /locus_tag="PLT31_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HPW94300.1"
                     /translation="MTSKPSLQERFTNWYIPLICKHKFKALVVYLFLALLCAYPILIN
                     PGLKLDADLSNLLPEKTPSVVALKESYTRFGSTDRFMLAVQSEDVELVAALQDSIQNY
                     IHKNWKGDFVSTQVDNDNKFFKDNALLYLPTKYLERIRDNLEDVQYEIGRKNLPLLVD
                     LVEAEPTKERVWFDASIPQELGLPDEAAGAFDAFFKETNKDTAVTNAKPEWDQKGPVP
                     "
     gene            complement(728..2659)
                     /locus_tag="PLT31_03845"
     CDS             complement(728..2659)
                     /locus_tag="PLT31_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPW94301.1"
                     /translation="MNPFILISSVALVLQSIMLVLFYSLPDPLGLSLTEMFSGKTIWQ
                     ISMAFGAILVLSSIFAFLKKPRLLAIFLAFYFLIAVVDYEVFRFSHQRLSYSFLRTYF
                     HITNITDSTTISTLGGDLKGTILWLMIMLITVVSAITFVVYYTKKKTKLAQTLKKSSL
                     KLPIVLGSVGLILSVIPLVLFLTGTRGIKTIPIINVPVDMRFTLGKHTLTSPVLHIAA
                     VETFEFIRDNHKITDELVDDLDAFLPSHFGSSRVDAKRYPLYRNAPTHEYKAKQPYNI
                     IFVFGESYKGRIFNQMLEGDTTLAPNLWKLASGAFFTEGGGGLWFKNAFSGGYPTVRG
                     IMATYLGFPSHPNRDVPSFYASNHFTGFPEYLTHYIKSYMTVSNPVFDHTLPFVEKFY
                     GDHWHLPETAEISGTADSLGMDLTLKLLDDLPTDTTWFLTYNTIASHIPFRGYPDDFA
                     SKPDDAMERYRNSILYIDHHFGRLLSALSSRPDFKRTVIVVMGDHDTPVDSIDYKVPQ
                     PLGVSSSQIFMGIFSADSNLFQGLEIREDVASQLDIGPTILDLAQVRASNHFWGYDLL
                     AEKRPAEQPAVFFSQNAYYLGFRDFVLTGGLTTEDVYKGVNDHFTLAEDSLSLFWKKK
                     VVGASKVLRSLLRNDNMQP"
     gene            complement(2656..3849)
                     /locus_tag="PLT31_03850"
     CDS             complement(2656..3849)
                     /locus_tag="PLT31_03850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPW94302.1"
                     /translation="MNLLFNLVAVQPIHNAKFHGGGSYGEEIFWALIRRLESSVFQNQ
                     ELQIFCAYDSKKYLDEKILTACQKYEIPLLDIHEYTPQQMVNQHAINTFYTPLYSLEK
                     KWQIQVERFVFTWHGVRALEMHYSHLGVAFGKTISRKLEALIRYRECWKKYFYKPKYQ
                     LLAKQIAEGRVQAMTVSEHSKASIQSFFPELMHVEIPVFYSPLAENEPIGELPKGVEN
                     KKYFLLTSGARWEKNNLRAVMAFDDLISSWKAQNKSFDYKVIVTGVTNPKVYTKKIRH
                     HDYFIFLNYIENEALEYLHQNAYAFIFPSLNEGFGYPPVQSMRYGVPVAASGTTSIPE
                     IGGDAVLYFDPYSITEIKNRLIQLLDRTIYNDYSKRVNNRYNEVCQRQKEDLDKAVHF
                     IIGLT"
     gene            complement(3846..4853)
                     /locus_tag="PLT31_03855"
     CDS             complement(3846..4853)
                     /locus_tag="PLT31_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94303.1"
                     /translation="MKYLFVLTSSPKDFFCEQTLVAIASLRVHNPKAFVTLLIDDKTA
                     ESLTGNRAVLKEAVDELKVLTLDESLSPMLRSRYLKTVMRNEIDGDFLYMDSDIAIVG
                     DLSIPEEWRGGIYAVLDFHTNLHKAINRKKVLNNAKMMGFSPILNDEIFNGGVMYASD
                     TPDCRQFFKTWHELWLYCVSKNFPYDMASLAETNFKFGYITKKMPGEWNCQLAYGRQF
                     IPNGKVLHFFGSRIIDTCGRYVPKSMDVFLPKILRRDFYTNLKNIPVTVNADKSININ
                     EYYDDVIAHVKEAFLDQEKHVGGFGADIVRSYAFAKSLGFLYKKMPFLVRPLELLGRL
                     L"
     gene            complement(4853..5830)
                     /locus_tag="PLT31_03860"
     CDS             complement(4853..5830)
                     /locus_tag="PLT31_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94304.1"
                     /translation="MGTKFLYILVSQEKDIFYEQTLVSAVSLRYHNPDAFISLLVDDL
                     TDKNLVNFRGQIKELVNEYKVVEYPKETSNKVRSRLLKTNMRNLIDGDFLYIDGDTAI
                     VDKLDAKFGEECDVGAVSDLHARENDKYHTKHKLINARIKKLNFSFSLKNLYFNGGLI
                     FAKDSLKAKEFFNKWHELYLYCVTKGIDVDQLSLNESNHVLGFPIKEMPGEWNCQVRE
                     AYNHLYRVKTIYPILCRAKIIHFFGSGIDGRSEPHPLMKNEFFEKIKREGKISDEALQ
                     IIYNAKSAFICAPKVLNPSVRFPLFFIFRQFPRLCRILMQIKKQVFGYK"
     gene            complement(5849..6781)
                     /locus_tag="PLT31_03865"
     CDS             complement(5849..6781)
                     /locus_tag="PLT31_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94305.1"
                     /translation="MRYHYVFMSQWDHLNEVIYGDILHLENVEFLEAPANNWSIKLMK
                     LLPNPLKFLWNKKFYNKKIDGPICFVFRMSSMMKGREPLFRYLKKRYKGCKFVAYLGD
                     VIASRPYADLSLLDKYFDLVFSFDQGDCEKYSEHHLLYFPTMYSKQEIPDSSAFAESD
                     VFFCGKAKDRYECIAEIYKRLTASNIRCDFSVSRLNNGVHLVDGINYIDKMEYKEYLQ
                     RLDKSKCLLDVLQGESRGFTLRVWEALVYGKKLITTNSEILNAPFYDARQFLYISSPE
                     ILDVSFVTQNFQPEPRYISEVSPMKFLQFLENNL"
     gene            complement(6778..8334)
                     /locus_tag="PLT31_03870"
     CDS             complement(6778..8334)
                     /locus_tag="PLT31_03870"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HPW94306.1"
                     /translation="MDKVKKSHAEDNKRLAKNTVALYVRNAVTMPVALYVSRVLLKML
                     GVDDFGIYNVVGSIVILFSFMSTAMSSASQRFITYELGTGDMQSTNRVFCASFMAQIF
                     IAVILIAIVEVFGYWFLNYKLNIPEGRLMAANYAFQFSIFAFAIRILQVPYEATVVAN
                     EKMTFFAYASVADSLMKLGLVFLLKLSDYDKLITYAALIAAESFLMWFVYRLYCKRHF
                     STCDFHIVRDKSLFKRLFSFTGWSLWGSGANVLTQQGFTFILNIFYGVAVNAAMGIAN
                     QINSAMNSFVGSFQTSYRPQIVKAYARNDYEHLNDLVFSTSKMSYCLMVLPTLLLIFN
                     MPLVLNVWLSNPPPYAIQFCQMILICIIIDATTGSYNAAIMATGKIRNYQLAISASFF
                     LDLVMSFLLIKAGVLPYLVLISRIATRGLFNMFIGLFFLKSMLLFRIKNYLRHVIAPI
                     VAQLSIMVPVLSFFYGRYDSWTMLAISSLVVITLGFITIIYVVFNSQERSYAFGIFKN
                     KWLAFRGVKK"
     assembly_gap    8570..8669
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8670..10370
                     /locus_tag="PLT31_03875"
     CDS             <8670..10370
                     /locus_tag="PLT31_03875"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HPW94307.1"
                     /translation="LPFTILDNEVQRFLGSHLSFGLLNTYSDTSSVIMFWDYVANDYS
                     VPFLQFFVLALILPFTYGVYRLLCLWIAPFTKELDPPKDAKLKCKISIPKAAMAMLIF
                     YILSYLFIHVIWTGNARMTKLRPVVSLVYHELFTKSNSQGLTPKEYKEYGKTYQKLWQ
                     QIEGDSLWAFSTKDDTSAHPLYRIPTEKLLQSKKLQEQRAQKPNFIVVFLESHRGLNT
                     GFLNPDLKPSPTPFFDSLAQYSRVWERMHTSGLPTTGGLLSSHTGLPHHSKLSQATDL
                     AHISIPSYASVLKDSGYTTHYFSAADPAWDNLGVWMAKWYNAQHYDRTREDDSTFFIH
                     TASYILDTLAKQEAPFLTTIMTRSNHYPFNFAAGMTDEDKQKPLIERINITMNYTDRQ
                     LARFIRSIQNEPWFQNTYLIVLADHGFPLGENGVSTMNGGGFSNATWIPFLVWGQGIQ
                     TGRDTVTTAQIDIAPTILELAGIATPNYFMGHNLLRGFGSGLSLGAYSRVASIGLDGY
                     RLITKYPLSDGNTPWLFGESDTHQKMDLASEKGDVVEKLKKTLDTLILFSDYSLEKGM
                     "
BASE COUNT         3476 a         2112 c         1791 g         3136 t
ORIGIN      
        1 agggtactgg gcctttttga tcccattctg gcttggcatt tgtgactgcc gtatccttat
       61 ttgtttcctt aaaaaacgca tcaaaagcac cagccgcctc atctggaagc cctagctctt
      121 ggggaatact agcatcaaac caaacacgtt cttttgttgg ttctgcttct actaaatcca
      181 caagaagggg caagtttttt ctacctattt catactgaac atcttctaag ttatctctta
      241 tacgctctaa atattttgtg gggagatata aaagggcatt atccttaaag aatttattgt
      301 cgttatcgac ttgcgtagaa acaaaatcac ccttccaatt tttgtgaata taattttgta
      361 tagaatcttg caaagcggca actaattcta catcttcact ttgaacggcg agcataaaac
      421 gatctgtact gccaaatcgc gtgtaagatt ctttaagagc aacaacacta ggcgtttttt
      481 ctggaagtag gttagataaa tcggcgtcta attttagccc aggattaatt aaaatcggat
      541 aagcacagag gagagctaaa aacaaataaa cgacaagcgc tttaaattta tgtttgcaaa
      601 ttagtggaat gtaccaattt gtgaatcttt cttgtagaga tggttttgat gtcatagcta
      661 aaaatatata aaaagtatca acttggttca tgaaaatcaa tccccatcat agccaaagtc
      721 ttgcacctta tggttgcata ttatcattac gcaatagaga acgcaacact ttacttgccc
      781 ccactacttt tttcttccag aaaagcgaaa gagaatcctc tgcaagagta aaatgatcat
      841 ttaccccctt ataaacatct tctgtagtaa ggcccccagt taagacaaaa tccctaaatc
      901 ccaaataata ggcgttttga gagaaaaata cggcgggttg ttcagcgggg cgtttttcag
      961 caagtaaatc gtatccccag aaatggttag aagctctcac ttgagctaaa tctaaaatgg
     1021 ttggaccaat atctagctga gaagcaacat cctctcgaat ttctaaacct tgaaataaat
     1081 tcgaatctgc agaaaagatg cccatgaaaa tttgagaaga agagacgccc aaaggctgcg
     1141 gcactttata atcaatagaa tctacagggg tatcatgatc tcccatcact acaatcaccg
     1201 tacgcttaaa atcaggacga ctagaaagtg ccgaaagtaa tcgaccaaaa tgatgatcta
     1261 tatataaaat ggagtttcta tatcgttcca tggcatcgtc tggtttagaa gcaaaatcat
     1321 ctggataacc acggaatgga atatgcgatg caattgtatt ataagttaaa aaccaagtgg
     1381 tatcagtcgg taaatcatcc aaaagtttta atgttaaatc cattcccaaa ctatctgctg
     1441 ttccactaat ctcagcagtc tctggcaagt gccaatgatc accataaaac ttttctacaa
     1501 aaggaagcgt gtgatcaaaa acgggattgg aaacggtcat ataagatttt atgtaatgcg
     1561 ttaaatattc agggaaccct gtaaaatgat tagaggcata aaaacttggc acatctcgat
     1621 tggggtgcga agggaaccct aaataagtag ccataatccc tctgaccgta gggtatccac
     1681 cgctaaaagc attcttaaac caaaggccac caccgccttc tgtaaagaaa gcaccgcttg
     1741 cgagtttcca caaattagga gctagagtgg tatctccttc tagcatttga ttaaaaatac
     1801 ggcctttata agactctcca aacacaaaga ttatgttata tggctgtttt gccttgtatt
     1861 catgagtagg cgcatttcga tataatggat accgctttgc atccacgcga ctagacccaa
     1921 aatgagaagg taaaaaagcg tctaaatcgt cgacaagttc gtctgtaatt ttatggttgt
     1981 ctcgaataaa ctcaaatgtt tcgacggcag cgatatgtaa aactggagaa gtgagagtat
     2041 gcttaccaag tgtaaagcgc atatcgactg ggacatttat aataggaatc gttttgatgc
     2101 cacgagtccc tgtcaaaaat aaaactaaag ggataaccga aagaattaat ccaacacttc
     2161 ctaacacgat gggtaactta agacttgatt ttttaagtgt ttgagccagc tttgttttct
     2221 tcttagtgta atacaccaca aaagtaatag cacttacaac cgtgattagc ataatcatta
     2281 gccataaaat ggttcctttg agatcgccgc caagagtgga aatggtagtg ctatcggtga
     2341 tgtttgttat atgaaaataa gtgcgtaaaa aagagtaaga taatcgctgg tgagaaaatc
     2401 gaaaaacttc ataatcgaca acggcgatga gaaaataaaa agctaaaaag atggcaagca
     2461 aacgaggttt ttttaagaat gcaaaaatcg aagaaagaac caaaatagcg ccaaaagcca
     2521 tagaaatctg ccaaattgtt tttccagaaa acatttcggt taacgaaagc ccgagaggat
     2581 ctggcagaga ataaaaaagc acaagcatga tgctttgtaa aacaagggcc actgaggaaa
     2641 ttaaaataaa aggattcatg ttaaaccaat aataaaatgt actgctttat ctaagtcttc
     2701 tttttgcctt tggcaaactt cattgtatcg attgtttact cgttttgaat aatcattata
     2761 gatggttcta tcgagtaatt gaataagtct attttttatt tcagtaatag agtaagggtc
     2821 aaaatagagc accgcatcgc caccaatttc tggaatagaa gtggtgccac tggctgctac
     2881 aggcactcca taacgcatag attgcactgg tgggtaacca aagccttcgt ttaaagaagg
     2941 gaatataaac gcataggcat tttgatgcaa atattcgaga gcttcatttt caatgtaatt
     3001 caagaaaata aaataatcgt ggtggcgtat ttttttggtg tacacttttg gatttgtaac
     3061 acctgtaaca atgaccttat aatcaaaact tttattttga gctttccaag aagagattaa
     3121 atcatcaaaa gccattaccg cacgcaaatt gttcttttcc cagcgagcgc cactcgttaa
     3181 aagaaaatac tttttatttt ctaccccttt aggtaattct cctataggtt cgttttcggc
     3241 aagcgggcta taaaacacag ggatttcgac atgcattaat tctggaaaaa aagattgaat
     3301 agaagcctta ctatgttcac tcaccgtcat ggcttgaaca cggccttcgg cgatttgttt
     3361 tgcaagtaac tggtattttg gtttataaaa atattttttc caacattctc gatacctaat
     3421 caaagcttct aattttcgag aaattgtttt tccaaaagca actccaagat gactataatg
     3481 catttctagt gcgcgcaccc catgccatgt aaacacaaag cgttctacct gtatttgcca
     3541 ttttttttca agcgaataaa gtggtgtata aaaagtatta atggcgtgtt gattcaccat
     3601 ttgttgcggc gtatattcat gaatatctaa aagcggtatt tcatattttt ggcatgccgt
     3661 taaaattttt tcatcaagat attttttgct atcataagca caaaatattt gaagttcttg
     3721 attttgaaat accgaagatt ctaaacgcct aattaaagcc caaaagattt cttcaccata
     3781 agagcctcct ccatggaatt tggcgttgtg tataggctgc acggccacca gattaaaaag
     3841 taaattcata ataaacgacc tagcaattcc aaaggacgaa ctaaaaacgg catcttttta
     3901 tataaaaaac caagtgattt tgcaaaagca taagagcgca caatatcagc accaaaaccg
     3961 cccacatgtt tttcttgatc aagaaaagct tctttcacat gagcaatgac atcgtcatag
     4021 tattcattaa tattaatact cttatctgca tttacagtca cagggatatt tttaagattc
     4081 gtataaaaat ctcgacgaag aatctttggt aaaaacacgt ccatactttt aggcacatag
     4141 cgcccgcaag tatcaatgat acgagaacca aagaaatgca aaacctttcc atttggaata
     4201 aattgccttc cataagctaa ttggcaattc cattcgcctg gcattttctt tgtgatataa
     4261 ccaaacttaa aattcgtttc tgcaagagaa gccatatcat aaggaaaatt tttagagaca
     4321 cagtacaacc aaagttcatg ccatgtttta aaaaactggc gacaatcagg cgtgtcactt
     4381 gcatacatca ctcccccatt gaaaatttca tcattcaata ttggagaaaa gcccatcatt
     4441 tttgcattgt tcaatacttt ttttcgattg atggccttat gcaaattagt atggaaatca
     4501 agtacggcat aaattccacc tcgccattct tctggaatag ataaatctcc aactattgca
     4561 atatcggaat ccatgtacaa aaaatcaccg tcaatctcat tacgcatcac tgttttaagg
     4621 taacgcgagc gaagcatagg agaaagagat tcatctaaag ttaaaacctt taattcatct
     4681 acggcctctt ttaaaaccgc acgattccca gttaatgact ctgccgtttt atcatctatt
     4741 aaaagcgtaa caaaagcttt aggattatga actcgtaaag aggcaatcgc cactagcgtt
     4801 tgctcacaaa aaaaatcttt aggagaactt gttaatacaa ataaatactt catcacttat
     4861 acccaaaaac ctgttttttt atttgcatca aaatacggca aagccttggg aattgacgga
     4921 aaataaagaa caacgggaat cgtacagaag gattcaaaac cttcggtgca caaataaaag
     4981 ccgattttgc attataaata atctgtaatg cctcatcact aatttttcct tctcttttta
     5041 ttttctcaaa aaactcattt ttcattagcg gatgaggttc acttcttcca tctattcctg
     5101 aaccaaaaaa atgaataatc tttgctctgc ataaaatcgg ataaattgtc tttactcgat
     5161 aaagatgatt gtatgcttca cgcacttgac agttccattc cccaggcatt tctttgatag
     5221 gaaaaccgag aacgtggtta ctttcattta atgaaagttg atcgacatcg atcccttttg
     5281 taacgcagta caaataaagc tcatgccatt tattgaagaa ctcttttgcc tttaagctat
     5341 cctttgcaaa aataagtcca ccgttaaaat aaaggttttt aagtgagaag gaaaagttta
     5401 actttttaat tctggcattt atcagtttgt gttttgtatg atacttatcg ttttctcttg
     5461 cgtgtaaatc agacacggca cccacatcac attcttctcc gaactttgca tcgagtttat
     5521 ctacaatcgc tgtatctcca tcaatgtata gaaaatcgcc atcaatcaaa tttctcatat
     5581 ttgtttttag aaggcgagaa cgcactttat tggatgtttc ttttgggtac tcgaccactt
     5641 tatattcatt cactaattct ttaatctggc ctcggaagtt tacgaggttt ttgtctgtta
     5701 gatcatctac taacaaagaa ataaaagcat ctggattgtg atagcgaaga gagacagcag
     5761 aaacaagcgt ctgctcgtaa aagatatctt tttcctgact aaccaagata tataagaatt
     5821 ttgttcccat ttattttaat atagaagttt aaaggttatt ttccagaaac tgaaggaatt
     5881 tcattgggct tacttcgcta atatacctag gttctggctg aaaattttgc gtaacaaagc
     5941 ttacatctag aatttctggt gaactaatat acaagaattg ccttgcatca taaaaaggtg
     6001 catttaaaat ttcgctatta gtcgtgatta attttttccc ataaacaagt gcttcccaca
     6061 ctcgtaaagt aaatcctctg ctttctcctt gtaaaacatc taaaaggcat tttgatttat
     6121 ctaaacgctg aagatattct ttatactcca tcttatctat ataattaatg ccatccacca
     6181 aatgaacgcc attattcaat cgagagacgc taaagtcaca acgaatattt gaagctgtta
     6241 accgtttgta tatttctgcg atacactcat aacggtcctt agcttttcca caaaaaaaca
     6301 catcgctttc tgcaaaagca gaactatctg gaatttcttg tttagaatac atcgttggaa
     6361 aatacaacaa atgatgttcg ctatattttt cacaatcgcc ttgatcaaaa gagaatacta
     6421 aatcaaaata tttatccaaa agagataaat ccgcataagg cctgctagca atgacatcgc
     6481 ctagatacgc tacaaactta caccctttat aacgcttttt taaataacga aataaaggtt
     6541 cccgcccttt catcatggac gacattctaa aaacaaagca tattggccca tctatttttt
     6601 tattgtaaaa ctttttattc cacaaaaact tcaaaggatt tggtaatagt ttcatcaatt
     6661 tgatagacca attattagca ggcgcctcta agaattccac attttctaaa tgcagaatat
     6721 caccataaat cacctcgttc aaatgatccc attggctcat aaaaacataa tggtatctca
     6781 ttttttaacc cctcgaaaag caagccattt attcttaaaa ataccaaacg cataggagcg
     6841 ttcttgtgaa ttaaacacca catagataat agtaataaag ccaagcgtta ttactacgag
     6901 agacgaaatc gcaagcatcg tccaagaatc ataacgccca taaaaaaatg ataagactgg
     6961 aaccataatg gataattgag ctacaatcgg agcgatgaca tggcgtagat agtttttgat
     7021 tctaaataaa agcatggatt ttaaaaagaa gagcccgata aacatattaa ataatccacg
     7081 agtcgctatt cttgaaatta gcacaagata agggagaaca cctgctttaa taagcaagaa
     7141 actcatcact agatccaaga agaaactagc actaatcgcg agttggtaat tgcgaatttt
     7201 ccctgtagcc ataatcgctg cattgtatga acctgttgta gcatcaataa tgatacagat
     7261 taaaatcatt tgacaaaact gaatcgcata aggaggcggg ttactaagcc aaacattgag
     7321 taccaaaggc atattaaaga tgagaagcag cgtaggcaaa accattaaac agtaagacat
     7381 ttttgaagtg gaaaaaacaa gatcattcaa atgttcataa tcatttctcg catatgcttt
     7441 tacaatctgc ggtcggtaag acgtttgaaa gctaccgaca aagctgttca ttgcagaatt
     7501 gatctggtta gcaatcccca tagcagcatt cacagccaca ccataaaaga tattgagaat
     7561 aaacgtaaaa ccttgctgcg taagaacatt ggcgccactt ccccacaagc tccatccagt
     7621 aaaagaaaac aatcgtttaa ataaactttt atctcgaaca atgtggaaat cacaagtaga
     7681 aaaatgcctt ttgcaatata acctatacac aaaccacatc agaaacgatt cagcagcaat
     7741 tagagctgca taagtaatca atttatcata atcacttaat tttagaagga acacaaggcc
     7801 taatttcata agagagtcag cgacactggc ataagcaaaa aaggtcattt tttcattcgc
     7861 gactacggtc gcttcatagg gaacttgcaa aatgcgtata gcaaaagcaa atattgaaaa
     7921 ctgaaatgca taattggcag ccattaggcg gccctctgga atattcaatt tatagttcaa
     7981 aaaccaatat ccaaatactt ctacaatcgc gatcaaaatc acagcaataa aaatctgtgc
     8041 cataaaagaa gcacaaaaca cacggtttgt cgattgcata tccccagttc caagctcata
     8101 agtaatgaaa cgctgagatg cagaagacat cgctgtgctc ataaaagaaa ataaaatgac
     8161 aatactacct acaacattgt aaataccaaa atcatccacg ccaagcattt tgagcaatac
     8221 gcgcgaaaca tacaaagcca caggcatagt aacggcattt cttacataga gagccaccgt
     8281 gtttttagct agtcgcttat tatcctcagc atgggatttt ttcactttat ccatttctat
     8341 tataattatt aatctctggt aaaaatgtag tatagaaaat agaaaagaca gggtctttca
     8401 tcagaagaac tagccattat tgttattatt ttgatataga cgcaaatcat aagagcacca
     8461 tttttttaca tttcatctaa tgaaattttt ttctaatgcc atcacgagtc aaatcaagcc
     8521 tttatttaat tcgttagaga aattaattcc tctttcttta gcggtatggn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ttccttttac catattagat aacgaggtcc
     8701 agcgtttcct tggaagccac ctttcttttg gcctcttaaa cacatacagc gatacctctt
     8761 cagtaataat gttttgggat tatgttgcaa acgattattc tgttcccttt ttgcaattct
     8821 ttgtactcgc cctcatttta ccctttactt acggcgttta ccgtttactt tgcttatgga
     8881 tagccccttt tacaaaagag cttgatccac ccaaagacgc caaattgaaa tgcaaaattt
     8941 cgattccaaa agcagcgatg gccatgctga tcttctatat cttatcttat ttatttattc
     9001 acgtcatttg gacaggaaac gcgagaatga cgaagctacg gcctgttgtt tctttagttt
     9061 atcatgaact ttttactaaa tctaattctc aaggccttac ccctaaagag tacaaagaat
     9121 acggaaaaac ataccaaaag ttatggcagc aaatcgaagg cgattccctt tgggcttttt
     9181 ctacaaaaga cgatacatca gcgcaccctc tgtatcgcat ccccacagag aagctcttac
     9241 aaagcaaaaa attacaagaa caacgtgccc aaaagcccaa ttttatcgtt gtctttttag
     9301 aatctcatag aggcttaaat acaggttttt taaaccctga tttaaaaccc tcgcccacgc
     9361 cattttttga ctcgctcgct caatattctc gagtttggga gcgcatgcac acgagcggcc
     9421 tccccacgac aggtggctta ctttctagcc atacaggctt accccaccat tcaaagcttt
     9481 ctcaggcgac ggatttagct cacatttcga ttcctagcta tgcttctgta ttaaaagact
     9541 ctggctatac cacacattat ttttctgcag cagaccctgc ttgggataat ttaggcgtgt
     9601 ggatggcaaa gtggtataac gctcaacatt acgaccgtac cagagaagat gactccacct
     9661 tttttataca caccgcttct tatatactag atacactagc caaacaagag gctccttttt
     9721 taacaacgat tatgactcgt tctaaccatt acccttttaa ttttgcagca gggatgacag
     9781 acgaagataa acaaaagcct ctcatcgaac gtattaatat tacaatgaat tataccgata
     9841 ggcaactcgc ccgttttatt cgttccatac aaaacgagcc ttggtttcaa aatacgtact
     9901 taattgtact ggccgaccac gggtttcctc taggagaaaa cggcgtttcc actatgaacg
     9961 gcggcggttt ttctaatgcc acttggattc cgttcttagt ttgggggcaa ggaattcaaa
    10021 ctgggcgaga caccgtcacc acagctcaaa ttgatattgc acccacgata ttagaactcg
    10081 caggcattgc caccccgaat tactttatgg ggcataattt attaagagga tttggcagcg
    10141 ggttatccct tggagcgtac tctagagtcg cttctattgg gcttgatggc taccgtttga
    10201 ttacaaaata tcctttaagt gatggcaata ctccatggct ttttggcgaa agcgacacgc
    10261 atcaaaaaat ggatttggct agtgaaaaag gcgatgtagt ggagaagcta aaaaagacgc
    10321 ttgatacgct gattcttttt tctgattatt ctctcgaaaa agggatgtga tttttttcat
    10381 ctcgttttat cacatcccat aaccacctta attgacgctc ggctcattac gaaaaaaata
    10441 acgggttaag atgggtcgtt tcaaaacgac tttatttcct cttcagagag acctgaggct
    10501 tttagaatag catcaattga aacgccttgt tcttttaata cttttgccat ttctcgttta
    10561 gcgttaagcg caccttgttc aattccttgt tcaattcctt gttcaattcc ttgtt
//
LOCUS       DAOOBB010000052        10355 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_8084_length_10355_cov_4.148350, whole genome shotgun sequence.
ACCESSION   DAOOBB010000052 DAOOBB010000000
VERSION     DAOOBB010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 10355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10355
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8084_length_10355_cov_4.148350"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..776)
                     /locus_tag="PLT31_03880"
     CDS             complement(138..776)
                     /locus_tag="PLT31_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94308.1"
                     /translation="MPKTQINLDGWQDYRGNYTGALLYVETSHQSAVPVRDQLNENGK
                     GFFYEPNYETNTYGFVSCSNVKNVNSIIKNKSRYILFGTRYEGVTYPDFKNKYLIMGY
                     MRIDKTKDVRTRHIQKYMGNPSEEEPECMQLTQDFAVYGEMHFVGLENSFILTDELLK
                     EWKYKGRVSRQLKTIFEKEHLDIILSHLNSMPDMTEEYIATVDEYKEALEEG"
     gene            complement(869..1681)
                     /gene="murI"
                     /locus_tag="PLT31_03885"
     CDS             complement(869..1681)
                     /gene="murI"
                     /locus_tag="PLT31_03885"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HPW94309.1"
                     /translation="MIGVFDSGFGGLTILRDLRKALPENDFLYLGDNARSPYGSRSFE
                     TIHRYTWQSVSYLLEQGCPLVVLACNTASARALRTIQQKDLVNSPYSGRVLGIIRPTA
                     EEIGFYTKTGHIGIFATPGTVYSESYLLEIRRFFPNIKVSQQACPLWVSLVENGEFDS
                     EGARFFIQRDVNALLEKDPEIDTILLGCTHYPLIADQIREFLPKNIQLVSQGKIVAEK
                     TLDYLKRHPEIDSKLQKSGKTNFLTTDTAEFFKKGAFLFGETAISVKSLTFL"
     gene            complement(1678..3822)
                     /locus_tag="PLT31_03890"
     CDS             complement(1678..3822)
                     /locus_tag="PLT31_03890"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPW94310.1"
                     /translation="MDIGEILKGLNSDQRKAVLHDHEKDAQLLILAGAGSGKTSVLTK
                     RIQYRIASGVCPDSILSLTFTAKAALEMKERVCQIFPEAGVRLCTFHSLALFLLREKV
                     GDSFGYERLGFQKPPIPVESDVGDFACDLVRFGLGAKSVSREELFSLDLSSRLERELI
                     PVRKSILLSGHVIFDDLIYLAIRLLENDPEFRAEFRGRWTEFLVDEYQDINPAQYRLV
                     IALLGDRKSLFVVGDDDQAIYGFRGADIGNIIRFQKDFKESTLIRLEWNYRSTPSILH
                     LANRIFTDKPVTLRKVLRAGNCRQDALFKENRKPEFWESENPVLEMSRIVQRIKEMRL
                     DYDLDYEAFAILVRYNHQRIYYEEALKDYGLPIFQVEEEGDVKGVHVETVHSSKGLQY
                     PVVFYAGLAEKLTPGSCRGSKKEQKKQIKEERRLFYVGVTRAEALLVLLYCSKRHWKG
                     RLETFRKSRFLKCLEKPLPESIMPLFLFRIFVLTKILCYMGIGILRLSVLRLVQPGKI
                     KQWVDLQIQAFGAYCIRSLRVDLTIEDQALLGKVDWSRPVVVIGNHQSYADIPVVFLA
                     LSRTVGFLAKRELKLIPFLGFWMKKIGCIFIDRNKKGAGLEVKKEILESKEIPCVFIF
                     PEGTRSKNGSMLPFKSGGFRMAVELEATLLPVVLHGTRAVWESRRDSKICKVSARILE
                     PLDTLALKKEGGEIKIREYLMPKIRKQMEDAL"
     gene            complement(3896..4873)
                     /locus_tag="PLT31_03895"
     CDS             complement(3896..4873)
                     /locus_tag="PLT31_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPW94311.1"
                     /translation="MNQTIKSTLVFAIKLLVTIIPAYFVYTNITQTPGWDSGDLFQLF
                     SQMDIWPLVVALVCLGVSNLTGCLQWKILLEKQDVHLSYGHLLKLYFVGLFFNNFMPG
                     NVGGDFKKVYDIRVQGGQDSVGAGLTATFFDRIFGLFFLNALALGVGALFFVYDEQQR
                     VFLLPSLWVFLGFCAFFATLFSKRLSKLLSRLTSYVFPNAVQDRFLRMQSRFQYFRKI
                     RLWTNITILSALTQILRVLVHFFCGLAIGLDIAVSWYFFYIPMIAVISALPISIGGFG
                     PRELLAQSLFAKAGVPGLESVLVQLLAYLASLLLSLFGAIFFWCDRKKA"
     gene            complement(4870..5979)
                     /locus_tag="PLT31_03900"
     CDS             complement(4870..5979)
                     /locus_tag="PLT31_03900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPW94312.1"
                     /translation="MKILVVNYRDRKHPASGGAEKHLHCIFSRIVEKGHQVVLFSTSF
                     PGAPSYEIVDGIEVIRSGSDLLFQLTYARAIKKLDRKYQFDVIYEDLNKLPLFTPQLS
                     KKPHIIQIHHLWKRSIFSEAFFPVALGVWLFEKMIPLFYKKSHFIVVSPSTEKELEEL
                     GIASSQIQLVYNGADGLPPNVTLPLKKSDYFLWLSRLHRYKGIYTALEAFRIFSKKYP
                     DVKLRIAGGGPLLKKMPKIIERYGLTGKVILEGYVSGEKKRTLLAEAIGLLQTSYKEG
                     WGLTVIEAAECKTATIASAVPGLVDSVIDQKTGILFPMKDASSCAQAMELFYLNSERR
                     ELFELEAQMHAAEFTWDKAALETLKILEWRVAFSK"
     gene            complement(5988..6764)
                     /locus_tag="PLT31_03905"
     CDS             complement(5988..6764)
                     /locus_tag="PLT31_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPW94313.1"
                     /translation="MTIKEPTQSVWNRFWQQKNDMEKVYPSSPSVFKSIEKHFNLDGM
                     KILEVGAGTGRDSVELARRGAEVYVLDFAENSLKIINSLRIKEGLYDQIHLIRGDAFA
                     SPFPDNTFDLVFHQGLAEHFKDPVALLRENYRIIKKGGHCLCDVPQTIHPYTVIKHIL
                     IALDRWFAGWETEYTMPQLKGLMTQAGFSIRYTYGDWMRPNLFYRMLRELCFRFNIEL
                     PKYPLTGTAYQKLKDQILDALATVPFMHYTQLSIGVLGKK"
     gene            6988..7800
                     /locus_tag="PLT31_03910"
     CDS             6988..7800
                     /locus_tag="PLT31_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HPW94314.1"
                     /translation="MHIDSTDTTLKRYLEDIRKTAPLSREEEQILFQKAKEGDKIARK
                     KLISANMRFVLKVAIQYRGCPIPLPDLVSEGAMGLVRAIESFEHTRGLKFISYGVWWI
                     KAYITRAINEQGNLIRLPANQHLRVRKALHEQSRGKEINEEIRELIQIGQRGVSFDSP
                     LKADSKATYAEVLADNTTTNPEADSEIQSVEALARDLMEQLPEREAKVITGIFGINQE
                     APQTLREVGESMNISHERVRQLRDQALRRIRKYNSREFLQEKKDAFLAAINK"
     gene            complement(7852..>10355)
                     /locus_tag="PLT31_03915"
     CDS             complement(7852..>10355)
                     /locus_tag="PLT31_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94315.1"
                     /translation="GTGSSSSGGGTGSSSSGGAGTGDDLSSSSSSGTGSGSGDDINFS
                     SSSSKQYVGFDDFDIDDDDDPLPYPSDDKREKGDTLVIGGSVLNPVDAEPGTPGTITI
                     GGNVYDTTLPGSSLDGFGGNTGSEKEAVNVGQVIEVTLSGDKLLDYLNISSGDPIIIT
                     GVGGIQIIDPNNPNDPSTKKTIISSGNDVKILITSDSVVMGGQIYVQNELGETVIFDG
                     FNFLDPIPDALVGFIKDTEGDGKLDMVEVVLNDPLPSKMTVTAMAIVVNGDTLNVTSV
                     PNLNKDRITVDVTSLNFPDKFPEDAYVIVTYTDTETNTSYKRNASLVEVGGHVIKQAN
                     AIRNASGQDSLFIEFNIDLLPADVSQADFIIMLNKNGFDLDQVSSVSMPTKNLIIITG
                     DSLGLRGADRDSISLYPSVSFTNLPYMTSDEYSRQIVVKVTDRLPTGILDQIVTVFPD
                     SISEEDKNKLYMSFPWYSFRGMLIQLQAQPASLEIDPKDPKRVIWNVQSDIKLATGLT
                     SISDEMPQATIYTYYDVMGYTFVAENTSPIRDKMAPVITAANLRYGDGVDTLIVSFSE
                     IVNSSKLSGKDYFSYIHGQDTINLVPSRIEWSNDGSSARLVLNTGFNTILPGDSLMMI
                     SGKDGSIKDNAGNIAGEIPSPVVITGFLNQLVHSVKMGSLNSEDETLRELSSVSLSYL
                     PSSTRKEDLEEKGVLGHLVELGQRFVPQLIDAAQVDPNGEVNPSILDSLDPAKVMLSF
                     SVRYFDHVGQYVNDTVISVACNSPKFGGNCLNTDQKLFVNWNFKDRSGRFVGAGVYLV
                     QFKLVVRYENKRIEEELIDKWGVRRSKKGTVPLNE"
BASE COUNT         3472 a         2168 c         1617 g         3098 t
ORIGIN      
        1 aaaagcagtg aaagaaatgc ttgaagccat ccgtcgagca gagcattaat tctcatacca
       61 tttctttgat tcttaaaaaa aaaaataaaa cccccactga ctaccagtag gggttttatt
      121 tttaatcttt agtaaaatca accttcttct aaggcttctt tatattcatc tacggtcgca
      181 atatactctt cagtcatatc tggcatagag tttaaatgag acaaaataat atctaaatgt
      241 tctttttcaa aaatcgtctt taattgacga gaaacacgac ccttatactt ccattctttg
      301 agtagttcat ctgttaaaat gaaactattt tctaaaccaa caaagtgcat ttcaccataa
      361 acagcaaaat cctgagttaa ttgcatgcat tctggctctt cctcacttgg attccccata
      421 tatttttgaa tatgccgagt acgaacgtct tttgttttgt caattctcat ataccccata
      481 atcaaatact tattcttaaa atcaggataa gttacacctt cataacgtgt tccaaaaaga
      541 atgtatcggc ttttattttt gattattgaa ttaacgtttt ttacattaga acaacttacg
      601 aaaccatatg tatttgtttc ataattaggc tcataaaaga aacctttacc attttcattc
      661 aactgatcac gaacaggcac tgcagactgg tggcttgttt caacatacag taaagctccg
      721 gtataattac cacgataatc ctgccaacca tcaagattta tttgtgtttt aggcatatct
      781 ttttccagtc aaaatgttta tagataaaaa aataaagagc gaacctatat attaagttcg
      841 ttcctaatat gttcaagata cattatacct acaaaaaagt caatgactta acagaaattg
      901 ccgtttcacc gaacaaaaac gccccttttt taaaaaattc ggcagtatcg gtcgttaaga
      961 agttagtctt tcccgacttt tgaagtttag aatcgatctc tggatgacgt tttaaatagt
     1021 ctagcgtttt ttcagctact attttgcctt gagaaaccaa ttggatgttt tttggtaaaa
     1081 attcacgaat ttggtctgca attaatggat aatgggtgca tccaagcaag atcgtatcaa
     1141 tttctgggtc tttttcaagt aatgcgttta catctctctg aataaaaaaa cgagcccctt
     1201 cggaatcaaa ttctccgttt tctacaagag aaacccaaag agggcaggct tgttgagaaa
     1261 cctttatatt tgggaaaaaa cgacgaattt caagcaaata actttctgaa taaacagtac
     1321 caggagtcgc aaaaataccg atatgccccg ttttcgtata aaaccctatt tcttctgcgg
     1381 taggacgaat aatcccaaga actcgaccag aatagggtga attcactaaa tctttttgct
     1441 gtattgttct taatgcacga gcagaagccg tattacaagc aagaacaaca agagggcacc
     1501 cttgttctaa taaataggat actgattgcc aagtgtatcg atgaatcgtt tcaaaacttc
     1561 tagatccgta aggagagcga gcattgtcac ctaaatacaa gaaatcattt tctggaagag
     1621 cttttcgcaa atctcttaaa atggttaatc caccaaaacc agagtcaaaa acgcctatca
     1681 taaagcgtct tccatttgtt tccgtatttt aggcattaaa tattctctaa tcttgatttc
     1741 cccaccttct ttttttaatg ctaaagtatc taaaggttca agtattcttg cagatacttt
     1801 acaaatctta gaatcgcgac gagattccca taccgctctt gttccatgaa gaactactgg
     1861 caaaagtgtc gcttctaatt cgacagccat cctaaaacca ccgcttttaa acggaagcat
     1921 tgagccattt ttactacgag tcccttcagg aaaaataaat acacaaggaa tctcttttga
     1981 ctcaagaatt tcttttttta cttcgagccc agcgcccttc ttatttcgat caataaaaat
     2041 acaacctatt tttttcatcc aaaagccaag aaaaggaata agctttaatt ctcgttttgc
     2101 caaaaagcca accgtacgag ataaagctaa aaaaaccaca ggaatatctg catacgattg
     2161 atgattacct ataacaacta caggcctaga ccagtctacc tttcctaata gggcttgatc
     2221 ttcaatagtt aaatctactc taagagatct aatgcaatag gcaccaaacg cttgtatttg
     2281 caaatcaacc cattgtttaa ttttacctgg ctgcactaag cgtaacacag aaagtcttaa
     2341 tatacctatg cccatatagc acaaaatttt tgttagaacg aagatacgaa ataaaaacaa
     2401 tggcatgata ctctccggca aaggcttttc aagacatttc aaaaatcttg attttctaaa
     2461 tgtctctaaa cgacctttcc aatgtcgttt gctacaatat aataaaacta acaaagcctc
     2521 ggctcgagtc acccccacat aaaaaagcct acgttcttct tttatttgtt ttttctgttc
     2581 tttttttgaa ccccgacaag aacctggagt caatttttcg gctaaaccag cgtaaaaaac
     2641 aactggatat tgtaatcctt tagaagaatg cactgtttcc acatggactc ccttcacatc
     2701 tccttcttct tcaacttgaa aaatgggtaa cccataatct tttaaggcct cctcataata
     2761 aattctttga tgattgtagc gaacgagaat ggcaaatgcc tcataatcca aatcataatc
     2821 gagcctcatc tcttttatcc gttgcacaat cctactcatt tcaagaacag ggttttcact
     2881 ttcccaaaat tctggttttc gattttcctt aaaaagagca tcttgtctac agttacctgc
     2941 tcttaacact tttcgtaacg ttacaggctt atctgtaaaa atgcgatttg ccagatgtaa
     3001 aatagaagga gtagagcgat aattccattc taaacggatt aacgtacttt ccttaaaatc
     3061 tttttgaaag cgtataatat taccaatatc tgctcctcta aagccatata tagcttgatc
     3121 atcatctcca accacaaata aactttttcg atctcctaat aatgcaatca ctagtctata
     3181 ctgagctgga ttaatatcct gatattcatc aactaaaaac tcagtccatc gcccgcgaaa
     3241 ttcagctcta aattcaggat cattttctaa tagacgaatg gctaaataaa tcaaatcatc
     3301 aaagatcaca tgcccactta atagaatcga ctttctaaca ggaatcaatt ctctttccaa
     3361 ccgactggat aaatctaagg agaaaagttc ttctctagat actgattttg cccctaagcc
     3421 aaaacgaact aaatcacaag caaaatcacc gacatccgat tccacaggga tcggtggttt
     3481 ttgaaagccc aaccgctcat acccaaaaga atcccccact ttttccctta ataaaaatag
     3541 agctaacgaa tgaaacgtac ataaacgaac accagcctct ggaaaaattt gacaaactcg
     3601 ttctttcatt tcaagagccg cttttgctgt aaacgttaac gacaaaatgg aatcagggca
     3661 tactccagac gctatccgat attgaattct ctttgttaaa acggaagttt ttccagatcc
     3721 cgctccagct agtattaata attgagcatc cttttcatga tcatgtaaaa ccgcttttct
     3781 ttgatctgaa tttaatcctt ttagaatttc tcctatatcc ataaaacatt ccttttaaaa
     3841 ggctaatggt aaaacaagcc ttctttttaa acgtttttag aagagaaaaa ttcaactatg
     3901 ctttttttcg atcgcaccaa aaaaagatag caccaaagag actcaggagt aaacttgcta
     3961 aataagctaa aagctgtact aatacagact ccaaaccagg aacgcctgct tttgcaaaaa
     4021 gagattgagc aagtaattct cttgggccaa agcccccaat agaaatagga agagcactaa
     4081 tcactgcaat cataggaata taaaagaaat accaagaaac agcgatatct aaaccaatcg
     4141 ctaaaccgca aaagaaatga accaataccc gaagtatttg agtcaacgca gataatatgg
     4201 ttatgtttgt ccaaagacga atttttctaa aatattgaaa tcgggattgc attcgcaaaa
     4261 agcgatcttg aacagcatta gggaaaacat aggaagtcaa tcgacttaat agtttactta
     4321 gtcttttact aaatagtgtc gcgaaaaatg cacaaaagcc taaaaagacc caaagagaag
     4381 gtaataaaaa gactcgttgt tgttcatcat aaacaaaaaa caacgctccc acaccaagag
     4441 cgagcgcatt taagaaaaat aagccaaata ttcgatcaaa aaaagtcgcc gttaaaccag
     4501 cccctacaga atcttgccct ccttggacac ggatatcata aactttctta aaatcgccac
     4561 ccacatttcc aggcataaaa ttattaaaaa ataaacccac aaaataaagc tttaataaat
     4621 ggccataaga aagatgaaca tcttgctttt ctaatagaat tttccattgg agacaaccag
     4681 tcaaattaga aactcccaaa caaacaagcg ctaccactaa aggccaaata tccatttgcg
     4741 aaaagagttg aaacagatct cctgaatccc aacctggtgt ttgagtgata tttgtataaa
     4801 caaaataagc agggataatt gtaactaaca atttgattgc aaaaactaat gtcgatttta
     4861 tggtttgatt cattttgaaa aagccactcg ccactctagg atttttaacg tttctaaagc
     4921 agccttatcc cacgtgaatt cagctgcatg catttgtgct tctaattcaa aaagttcacg
     4981 gcgctccgag tttaaataaa ataactccat cgcttgggca catgaagaag catctttcat
     5041 aggaaataga attcctgttt tttgatcaat cacactatcc actaaaccag gtactgctga
     5101 ggcaattgta gctgttttac actccgcagc ttcaattaca gtaagccccc aaccttcttt
     5161 atagctagtc tgtaaaaggc ctatcgcctc tgcaagcaac gttctttttt tctctcccga
     5221 aacataacct tctaaaatca ctttacccgt tagcccgtaa cgttcaatta tttttggcat
     5281 ctttttcaag agcggaccgc caccagcaat tcgaagtttt acatcagggt atttctttga
     5341 aaaaatacga aatgcttcta atgcagtata aatgccctta taacggtgta gacgtgaaag
     5401 ccataaaaaa tagtctgact tttttaatgg caaagtcaca ttagggggta aaccatctgc
     5461 cccattatac acaagctgaa tttgagagga agcaattcct aactcttcta attctttctc
     5521 tgtacttgga ctcaccacaa taaaatgact tttcttataa aacagcggaa tcattttttc
     5581 aaaaagccac acccctaaag ccactggaaa aaatgcttct gaaaatatcg atcttttcca
     5641 aagatgatga atctgaataa tatgtggttt tttcgataac tgcggtgtaa ataaaggcag
     5701 cttatttaga tcttcataaa taacatcaaa ttgatatttt cgatctaatt tttttattgc
     5761 tcttgcataa gtaagttgaa ataaaagatc tgagcccgaa cggattactt caattccatc
     5821 cacaatctca taagatggag caccaggaaa ggatgtcgaa aaaagaacca cctgatgtcc
     5881 tttttcaaca atacgagaaa aaatgcaatg caaatgcttt tcggccccac ccgaagcggg
     5941 gtgctttcta tcccgataat tcacgactag tattttcatt atctaatcta ttttttccct
     6001 aaaacaccaa tactcaattg agtgtaatgc atgaaaggca ctgtagctaa agcatctaaa
     6061 atttgatctt tcaatttttg ataagccgtt cctgtcaaag gatatttagg cagctcaata
     6121 ttaaatcgga aacacaattc tctaagcata cgataaaata aatttggacg catccaatct
     6181 ccatatgtat acctaatact aaaaccagct tgggtcataa gaccctttaa ttgtggcatc
     6241 gtgtattcag tttcccaacc agcaaaccat cgatcaagag caatgagaat atgtttaatg
     6301 acagtataag gatgaatagt ctgaggcaca tcgcaaaggc aatgcccccc tttttttatt
     6361 atccgataat tttcacgcaa taatgctaca ggatctttaa aatgctctgc taaaccctga
     6421 tgaaaaacta aatcaaacgt attatcagga aatggagaag caaaagcatc tccacgaatc
     6481 aaatgaattt ggtcataaag accttctttg attcgcaggg aattaatgat tttcaaacta
     6541 ttttcagcaa aatccaaaac ataaacttca gcccctcggc gagctaattc aacactatct
     6601 cttccagttc cagctccaac ttctagaatt ttcattccat ctaaattgaa gtgtttttct
     6661 atggatttaa aaactgaagg agaagatggg taaacttttt ccatatcgtt tttttgctgc
     6721 caaaaacgat tccaaaccga ttgcgtaggc tctttaattg tcataaacgc aaatatacga
     6781 attcagtgaa aaagagaaat acagacgttt tattttctta catttttgct attaatgtca
     6841 tttttatatt acttagtcat cttttaatga ctattttata tattaaaaaa cgccaaatta
     6901 tgataacaac aaatatttta cacttttggc acactccttg ctcattaaaa aagcgatatt
     6961 atggattgat aataaggatt tttaagcatg catatagatt ctactgatac tactctaaaa
     7021 cgttatctcg aagacattag aaaaacggcg cccctttcta gagaagaaga acaaattttg
     7081 tttcaaaaag caaaagaagg cgataagatt gctcgtaaaa aattaatttc tgcaaatatg
     7141 cgctttgtat taaaagtggc aattcaatat cgtggatgcc ctattcctct tccagatcta
     7201 gtgagtgaag gcgccatggg cctcgttcgt gccattgaat catttgaaca tactcgtggt
     7261 ttaaaattca ttagttatgg tgtttggtgg attaaagcct atatcaccag agccattaat
     7321 gaacaaggta acttaattcg tctaccagcc aaccaacact tacgtgtccg taaagctttg
     7381 cacgaacaat ctcgcggaaa agaaatcaat gaagaaattc gcgagttaat tcaaattggc
     7441 caaagaggtg tttcttttga tagccctttg aaagctgatt caaaagccac atacgccgaa
     7501 gtgcttgccg ataataccac tacaaaccca gaagctgatt ctgaaattca aagcgtagaa
     7561 gcacttgcta gagaccttat ggaacaactt ccagaaagag aagctaaagt gattactggt
     7621 atttttggta tcaatcaaga agcgcctcaa acacttagag aagttggcga atctatgaat
     7681 atttctcatg aacgcgtacg tcagcttcgt gaccaagcgc ttagaagaat tcgaaaatac
     7741 aacagccgcg aatttcttca agaaaagaaa gacgcattct tagcggctat caataaataa
     7801 aactttatga ataaaaaaaa gaaccccgat tggggttctt ttttttagat gttactcatt
     7861 gagaggcacc gttccttttt tactgcgacg gacaccccac ttatcaatca attcttcttc
     7921 aattcgtttg ttttcatagc gaacaacaag cttaaattga actagataaa ctccagctcc
     7981 tacaaagcga ccagatctat ctttgaaatt ccagttcaca aagagctttt gatctgtatt
     8041 caagcaattg cctccaaatt tagggctatt acatgcaacg cttattactg tgtcattcac
     8101 atattgaccg acatgatcaa aatatcttac actaaaggaa agcatcactt tagcaggatc
     8161 taatgaatct aatatcgaag gatttacttc tccattagga tccacttggg ccgcatctat
     8221 taactgcggt acaaagcgct gacctaattc cactaaatgc ccaagaacgc ctttttcctc
     8281 caagtcttct tttcgtgtcg aacttggtaa ataactcagc gatacagaac ttaattctcg
     8341 aagagtttcg tcttctgaat ttaaggatcc cattttcaca gagtgcacca attgatttaa
     8401 gaagcctgta atcacaactg gtgaaggaat ttctcctgcg atattgcctg cattatcttt
     8461 aatagatcca tcctttccag aaatcatcat gagagaatca cctggaagaa tagtattaaa
     8521 gcctgtattc aaaaccaacc ttgcagaaga tccatcgttt gaccattcaa ttcttgaagg
     8581 aactaagtta atcgtatctt gtccatggat ataagagaag taatctttac ccgagagttt
     8641 tgaagaattt acgatttcag agaatgaaac aatcaaagta tcaactccat ctccataacg
     8701 gaggtttgcc gccgtaatga ctggagccat tttatctctg attggagaag tattctcagc
     8761 cacgaaagta taacccatca catcatagta tgtatagatg gtggcttgtg gcatttcatc
     8821 actaatgcta gtaagaccag ttgctagttt tatatccgat tgaacattcc aaatgacacg
     8881 tttaggatct ttcggatcga tttccaaact tgcaggttga gcttgtaact gaattaacat
     8941 acctctaaag ctgtaccatg ggaaggacat atagagttta tttttgtctt cttcagaaat
     9001 actatcagga aatacggtta caatttgatc caaaatacct gttggtaaac gatccgttac
     9061 ttttacaact atctgacgac tgtattcatc tgaagtcata taaggtaaat tcgtaaacga
     9121 gacgcttggg tataaagata tactatctcg atcagctcct cttaaaccaa gcgaatctcc
     9181 agtgattata attaaatttt tagttggcat cgaaacggaa ctgacttgat ccaagtcaaa
     9241 cccattttta ttcaacataa tgataaagtc tgcctgagaa acatctgctg gcaataagtc
     9301 tatgttgaac tcaataaaca gagagtcttg accagaagca tttctaattg cattggcttg
     9361 cttaatcaca tgaccaccga cttccacaag agaagcatta cgtttatatg aagtattcgt
     9421 ttctgtatcg gtataagtca caatcacata agcatcttca gggaatttat ccgggaaatt
     9481 taagctagtg acatctacag tgatgcgatc cttattcaaa ttaggaacag aagtgacatt
     9541 taatgtatct ccattcacca caatagccat ggcagtcact gtcatttttg aaggtaatgg
     9601 gtcattaagc accacttcta ccatatccag ttttccatcc ccttcagtat ctttaataaa
     9661 gccaactaaa gcatctggaa taggatctaa gaaattgaac ccgtcaaaaa taactgtttc
     9721 acctaactca ttttgaacgt aaatctgacc gcccataaca acagagtcac tagtaatcag
     9781 aatcttcaca tcatttccag aagaaataat ggttttcttt gtacttgggt catttggatt
     9841 atttggatca ataatctgaa taccaccaac acctgtgatg attataggat caccagagga
     9901 aatattcaaa taatcaagca atttatctcc agagagtgtg acttctatga cttgtcccac
     9961 attcacggct tccttttctg atccagtatt tccaccgaat ccatctaaag aagaaccagg
    10021 taatgtcgta tcatagacat tgccgccaat cgtaattgtt ccaggagttc caggttctgc
    10081 gtccacagga tttaagacag agccaccgat aactaaagta tcgccttttt ctctcttatc
    10141 atcacttgga tatggaagag gatcatcatc gtcatcaata tcaaaatcat caaaaccaac
    10201 atattgctta ctagaagagc tcgagaaatt aatatcatca ccagaaccag aacctgttcc
    10261 tgaactagat gaactactta aatcatctcc cgtgccagca ccaccagagg aactacttcc
    10321 tgttcctcca ccagaggaac tacttcctgt tcctc
//
LOCUS       DAOOBB010000053        10303 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_8134_length_10303_cov_5.711456, whole genome shotgun sequence.
ACCESSION   DAOOBB010000053 DAOOBB010000000
VERSION     DAOOBB010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 10303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10303
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8134_length_10303_cov_5.711456"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PLT31_03920"
     CDS             <1..391
                     /locus_tag="PLT31_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94316.1"
                     /translation="AKIDPNLSFGFSYSPRPLSSNTAFARKVNFAVDFEDALNNDRNY
                     KPLSHLNLGMEVEQTLLAWPGLNNEIRALKVRLAGGFKGGYPSGGVSLEVLRLVEVEL
                     ATWAEEKGYYTGQAPYRIYMGQVRVGF"
     gene            complement(388..906)
                     /locus_tag="PLT31_03925"
     CDS             complement(388..906)
                     /locus_tag="PLT31_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94317.1"
                     /translation="MIRHFFQILFAGATALFWADKSKILMGIIFSFSAVQHIACTYGP
                     PNDIDNGNDPSPDCSPQFSVEKISRIDYSECENTLADLLENVSACEAPCKNGEMLNAT
                     WRPDGSIDSGQFVCYDERPEDCSYNTMLSKKDSIIALGDTCLLDHMKADSSLIQYLCG
                     TEVVSVEEADRR"
     gene            complement(985..2910)
                     /gene="recD"
                     /locus_tag="PLT31_03930"
     CDS             complement(985..2910)
                     /gene="recD"
                     /locus_tag="PLT31_03930"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HPW94318.1"
                     /translation="MGDTMENKKGSEILSEVLSPMDKHMLRFVKELQTDISENVENFL
                     KMIFLLQGAGNTRFSLDAAVFNEKWMVLWNGLQTMSLESFNDFIPTIKTALQDIQKNK
                     YTNMIEYREDNKETPDEAVTKPFVIHKNYIYITKYFDAKCIIERVANEIFKGGKAPEN
                     KSIQECIDYIASIHVKDSPFANAQNPEGKFLVNEEQAEAILRGQNENLIITGGPGTGK
                     TTVVLYILWKWLASNHEFLDWNIRLAAPSGKAADRMRESLINGLNGIEESVRKGSGEK
                     IYKKLSDLQSSTIHRLLKFIPTSGTFKYNKNNPFSENTLFVIDEASMIDISLFASFLQ
                     AVPKGAKVFILGDPFQLPSVGAGAVLGEILSQENKFSIKLKKSNRFTDESEIGQLAKA
                     IQQNAETTNFQPVSMQEQESVKFIDILARTIKETENQVKTLVDEWLSDMQQLPILATQ
                     VKVDLKKDEVSESEVCKKLWNLSLKKRMLSAERKGPRGVDFLNRQAFKNMMRTEAARK
                     HTDGKYFAGQLLILTQNQPMYQLYNGDTGIVVFSDNRPYLMLKKDKFVFYPLALLPAD
                     ALESAFAITIHKSQGSEYEEILMFLPSQIGHPLLTNQIVYTGITRAKKKVTTVVSPEA
                     FQAACSTVTKRETGVVL"
     gene            complement(2912..6544)
                     /locus_tag="PLT31_03935"
     CDS             complement(2912..6544)
                     /locus_tag="PLT31_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPW94319.1"
                     /translation="MSFIDKIDLEENWVVEASAGTGKTYTIQQIVARLVKEGISLKKI
                     LIVTYTEKAAGELKDRIRKKLEEVLDSQNNSQDDLDVFETALREVDNASIFTIHSFCQ
                     KALKEFAYDAGRPFDLNMIDDNEVLDVIDEWARDKWPHDPLYKMLIQHDTNVSDLKKE
                     LKNAIPLYYGKSADGEEIVEIDSAVIPNFPKWPEKFDDLLLVPEVKKNYDIIEANSGC
                     TFNKDNESKTLGDFAAALKRWQGTSSELYIAGDFSDGKTPKSWPSEVYGAFKFFYKLK
                     EEISKYPDAVKQKFIYSQIPLLFEEWQRIKKDHKSQSFNDMILSVHRAVMEKSLDGSD
                     SNLCKKLRSEYTHAIIDEFQDTNQLQWDIFKKIFICDKHSVLVVGDPKQSIYSFQGAD
                     VNVYRSAKKEINNSKELNNNYRSTNEMIEACNRFFSCKSFFGDNSSFHKSEPPESVEQ
                     QKNAARFWNENLKEWTNAVPFWISEESLSEESFAETCVRKIIECCSYVKTSNGLHTRL
                     QVFNKEKCKEWRDVTFKDFAILARTRSEMEPIEERMRKVGVPFTRYKDDHLFDGRECA
                     SWIALFKALNAPDFTSRNRRILNELLMTDFFAFSLEQVQSDSFDNPLCKEREMINNWK
                     MLALHRRFAELQECIYSDTSVEERLKDLSKLQNLAKLRQIGSYAIDYLYDHGCSLNEL
                     IRKLVAMQNRQGDADDRDGNLIAKGTDFEAVQVMTIHASKGLEFPVVISVAGFKQIPT
                     NRSGPFLYHDPNDPKKMRLGLGDDAKEARKKEELEEWSRLFYVDFTRASSVLMLPRYD
                     KWYNKSGFKKHFKFLGESMKAFCVDENSAYYTVMQTENSWDFNTEKNLKKMVQEKILK
                     PLSDREGKLIATPVSKEDQLVLIENLQKKVGELSIMQHSYSSLVGKVESSVSIDGSKA
                     LDKEGLSESYDIENELEKADAGIGEEELSIKYPRGSKLGNALHEVFELTTFHDFTVKY
                     PDLELAQNSAELCDRVETAFRKQSLPIWNHQEDWTNHTIQILWNTLNATLPTIEGGRK
                     LSDIDSKFKLTSIEPNCSKSEAQFNLNATAENNGEASEFLQNVCKGFIDLMFVRQDSS
                     GENRYSILDWKSDVIPSGDYSSESVQKRVDEDYSVQRVLYCYCLIKWLKQFYSGLSET
                     EIFDKHFGGIYYVFARGCKADSENGIYAHTWETYDALCESYEKVRTLMHKRRKKITGE
                     INGEEQ"
     gene            complement(6541..10116)
                     /locus_tag="PLT31_03940"
     CDS             complement(6541..10116)
                     /locus_tag="PLT31_03940"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HPW94320.1"
                     /translation="MLYLKFALNIEKLADEMISKIKLSWTNPLVAPIVIFPDPKLEYW
                     FRLHWVKTQGTLANFNSMMIDRFLMTILCPNDNTKKKLNADLLRSVIWAYLYKNYKSL
                     GDEVTRYLEVEGKLDEKHLFDLCSKMATLFLEYETSRPKGFVHKGSNLNEKGDLAEGI
                     LDRWKQGNLQNFFDGSEREVWQRKLYSQIFHTQGSEPSILTKAFENYNQRINKGKKIK
                     FETTYLTIPFLFQDCMLNHGKFHTEQFSINEKTLPVFIFGLTGMGQFYRVILQEYAKT
                     HDVNAYIQNPCMEFWEDTNVAKKSRHRQWNARNGVWQSENGEIPSTVRQKLHYSLNES
                     AGVEVNDDYEEGVNSRNENALLSYWGKSGRDNIKLWCQANDYNFEFEEESLELPSDSL
                     LHQIQYMVANRTNELPGLLSEDNESFSLTAAPTREREIEALHTRICKLLAKRDKDGNP
                     VNRTSDILVVSPNLDVYRTAIFQCFDQQSFQIPFSIVDSPAKKSLTGEALSALFAMQE
                     KRTLSRSIFFDLVRNPVIQQTRQISNEDVSAWESWVENMNVFRTRGNGKNDWTFGLKR
                     LMASRFSDHKISAAGDETSEGYAPYMDINSENDASLSRFADCVTELEEWIEFARNKET
                     VELGELDLISTMLNKWLAMTNPPDGMNGETIIFQNVLNSLEDLKFQFDAGATEVSWDC
                     ISQTLISAAEASEYSCGSLFMNGITFAKFVPNRIIPVKHLFFIGLDSGTFPGSKSQNT
                     LDLRRSVAPWPGDDSPVAKQRYAFLCQLMSVKKSLHISYLNKNIVKDEDLYPSSVVAD
                     IRNFLKNALRKNGIDVNAKKVWPQEVIPLDESRDYGELFTQKEFRNKSIFEDMKKSGD
                     ESDVEKEPLALSKELPPDRVSFYQFKKYLEDPFQFRVSQVLNDGEEDDDPTDEDFEPI
                     VFDILDKSSLMKQMVKFKITGDHEALEKLKWQMLSIGDIPDGVYGEKMWQDAEKYAEN
                     IIKLMEIDENSPKSNSTDWTFDERIELPMEQLDGTKWTLQGSLGWCNKKRNCLLYLTT
                     SDPHAAKTSPYFDKGKYLSSYVAALAIIASETSKYDDERHIEIQIYSGASSIKKPAKA
                     NVVLSPNKAIEILKKLYQKAFCEKYSKAVPADIVDETIETFSDYTKKFTGDHNLWKYF
                     KKAKNFDTRKDLGFSPNNFEEEWEKATKQQRNLMCFSIDGEENTK"
BASE COUNT         2951 a         2175 c         1861 g         3316 t
ORIGIN      
        1 agccaagatt gaccctaatt tatcatttgg ctttagttat agtccacgtc ctttaagctc
       61 caatacagct tttgcccgca aagtgaattt tgccgtagac tttgaagatg ctctaaataa
      121 tgatcgaaac tataagccat taagtcatct caatttaggt atggaagtag aacagactct
      181 tctcgcttgg cctggtctaa ataatgaaat tagagcccta aaagtaaggc ttgctggtgg
      241 atttaaaggt ggataccctt caggtggcgt tagcctagaa gtattacgtc ttgtcgaagt
      301 tgaactagcc acttgggcag aagaaaaagg ctattatact ggccaagcac cttatcgaat
      361 ctatatggga caagtccgag tgggattcta acgacgatca gcttcctcta cggaaacaac
      421 ttctgttccg cacaaatatt gaattaagga agaatccgct ttcatatgat cgagtaaaca
      481 tgtatcacca agagcaataa tagagtcttt cttacttagc atagtattat aagaacaatc
      541 ttctggacgc tcatcataac aaacaaattg accagaatca atagagccgt ctggacgcca
      601 agtggcattg agcatttcac cgttcttgca tggtgcctca caagcactta cgttttctaa
      661 aagatcggct aaagtatttt cacattcaga ataatcgata cgagaaattt tttctacaga
      721 aaattgtggg gagcaatctg gtgatggatc atttccgtta tctatgtcat ttggaggtcc
      781 ataagtacaa gcaatatgtt gcactgcaga aaaagaaaat ataataccca ttagaatttt
      841 ggatttatct gcccaaaata aggcggtagc tccagcaaat aaaatttgaa aaaaatgtct
      901 aatcatattt ctttatataa tatatattct tcactcactc ttttcaaaaa aaatacaagc
      961 tttataaaac cttatgaaat aaagttatag aacaacacct gtttcgcgtt tagtcactgt
     1021 gctacaggcg gcttgaaagg cttcgggcga aacgactgta gtcactttct tttttgcacg
     1081 cgtaatccct gtatacacaa tttggtttgt gagcaatgga tgcccgattt gcgaaggtaa
     1141 aaacattaga atttcctcgt attccgaacc ttgcgatttg tgaatggtaa ttgcaaaggc
     1201 actctctaat gcatccgctg ggagaagggc aagcggataa aagacaaatt tatccttttt
     1261 cagcatgagg tacggacgat tgtcactgaa aacaacaatt cccgtatcgc cattgtacag
     1321 ctggtacata ggttggttct gcgtaagaat taggagttgc cctgcgaaat attttccatc
     1381 tgtatgtttt cgagctgcct cagtacgcat catgttctta aaggcttggc gattcaaaaa
     1441 atcaacacca cgtgggccct tgcgctctgc agaaagcatg cgttttttta gcgatagatt
     1501 ccacagcttt ttgcaaactt cagattcgct aacctcatcc tttttcaaat ccactttcac
     1561 ttgtgtagcg agtatcggaa gctgttgcat gtcacttagc cattcgtcga caagcgtttt
     1621 gacctgattt tcagtttcct taattgtacg cgctagaata tctatgaact tgactgactc
     1681 ttgctcttgc attgatactg gttgaaaatt tgtggtttcc gcattttgtt gaatcgcttt
     1741 tgctagttgc ccaatttcag attcatcagt aaaacggttt gattttttta gtttaataga
     1801 aaatttattt tcttgagaaa gaatttcacc aagtacagcg cccgcaccaa cggaaggcaa
     1861 ctggaaggga tcccctaaaa taaaaacctt agctccctta ggaacagcct gcaaaaaaga
     1921 agcgaaaagg ctaatatcaa tcatgctcgc ttcgtcaatg acaaataacg tgttttcaga
     1981 aaacggatta ttcttattgt atttaaacgt tccagaagta gggataaatt taagaagcct
     2041 atggattgtg ctactttgta aatcactaag cttcttgtag attttttcgc cactaccctt
     2101 acgaacactt tcttcaattc cattcaagcc gttaattaaa ctttcgcgca tacggtctgc
     2161 ggccttacca ctgggggctg ccagtcgaat attccagtcc aaaaactcat gattgctagc
     2221 gagccatttc caaagaatat atagaaccac agttgttttt ccagttccag ggccacctgt
     2281 aataatcaag ttttcatttt gcccgcggag aatcgcttct gcttgttctt cgtttactaa
     2341 aaattttcct tcgggatttt gagcgtttgc aaatggcgaa tccttaacat gaatggaagc
     2401 gatataatcg atacactcct gaatactttt gttttcaggc gctttgcccc ctttaaaaat
     2461 ttcgttagct acgcgctcaa ttatacactt ggcatcaaaa tatttggtta tataaatgta
     2521 atttttatga atcacgaatg gttttgtaac cgcttcgtct ggggtttcct tgttatcttc
     2581 gcgatactca atcatgttgg tgtacttgtt cttttgaatg tcttgaagag cagtcttaat
     2641 cgtgggaata aagtcattaa aagactcgag agacatggtc tgtagcccgt tccaaagcac
     2701 catccatttt tcgttaaata ccgcagcatc cagagaaaaa cgcgtgttac cagcaccctg
     2761 cagcaagaaa atcatcttca aaaagttctc tacattttca gaaatatctg tctgcagttc
     2821 tttcacaaaa cgaagcatgt gcttgtccat cggcgaaagt acttcggaaa gaatctcgga
     2881 acccttttta ttttccattg tatcacccat tttattgttc ctccccgttt atttcaccag
     2941 ttattttttt ccgcctctta tgcatcagtg ttcgcacctt ttcatacgat tcacagaggg
     3001 catcgtaagt ttcccaagta tgtgcataaa tgccattttc agagtcggct ttacaaccgc
     3061 gagcgaatac gtaatagata cccccaaagt gcttatcaaa gatttcggtt tcgctaagac
     3121 cactgtagaa ctgcttgagc catttgataa ggcaatagca gtaaagaact cgctgtacag
     3181 agtaatcttc atcaacacgt ttctgcacac tttctgaact ataatctcca gaaggaatca
     3241 catcggattt ccagtcgaga atggaataac gattttctcc agaagaatcc tggcgcacga
     3301 acataagatc tataaagccc ttacaaacgt tttgtaagaa ttcgctcgcc tccccattat
     3361 tttcggcagt agcattcaaa ttgaactgtg cttcggattt actgcaatta ggttcaattg
     3421 aagttaattt gaatttggaa tctatgtcgg ataattttct accgccttca atcgtgggga
     3481 gagttgcatt taacgtgttc caaagaattt gaattgtatg gtttgtccag tcttcttggt
     3541 gattccagat gggtagagat tgtttacgga aagccgtttc tactctgtca cagagttccg
     3601 cagaattttg cgccaattca agatctgggt attttacggt aaagtcatgg aacgttgtga
     3661 gttcaaaaac ttcatgtaat gcgttaccga gcttagaccc tcgtgggtac ttgatagaga
     3721 gttcctcttc accaattcca gcatctgctt tttctaattc attttcaatg tcgtaacttt
     3781 ctgacaagcc ttccttatct agggctttac taccatcaat actcacgcta ctttcaacct
     3841 tacccacaag cgaactgtag gaatgctgca ttatgctaag ctcccctact ttcttttgca
     3901 agttttcgat gagaacaagc tggtcttcct ttgaaactgg tgttgctatg agttttccct
     3961 cacgatctga aagaggtttt agaatttttt cctgaaccat ttttttaaga tttttttcgg
     4021 tattaaaatc ccaagagttt tcggtttgca ttacggtgta ataggcagaa ttttcatcta
     4081 cacaaaacgc cttcatagat tcgccgagaa atttgaaatg ttttttaaaa ccacttttat
     4141 tataccattt gtcatatcgc ggaagcatta gtacagagga tgcacgcgta aaatcaacat
     4201 aaaacaagcg gctccattct tcaagttctt cttttttgcg tgcctcttta gcatcatcac
     4261 ctaatccgag tctcattttt ttgggatcgt tcggatcgtg gtataggaat ggcccacttc
     4321 tatttgttgg gatttgcttg aaacccgcaa ctgatattac tacaggaaat tcgagcccct
     4381 tggatgcatg aatcgtcatc acctgcacgg cctcaaaatc agtacccttt gcgatgagat
     4441 tcccgtcgcg atcatcggca tccccttgcc tgttttgcat ggccactaat tttcgtatta
     4501 gttcattcag cgaacaacca tgatcataga ggtaatcaat agcatagctt ccaatttgac
     4561 gaagtttagc aagattttgc agtttggaga gatcttttaa gcgctcttca acactagtat
     4621 cactataaat acattcctga agttctgcaa aacgccggtg tagagcaagc attttccaat
     4681 tattaatcat ttctcgctct ttacaaagcg gattatcgaa ggaatcactt tgaacctgct
     4741 ccaaagaaaa agcaaaaaaa tcggtcatca agagttcatt caatatacga cgatttcgtg
     4801 atgtaaaatc gggcgcattt aatgctttaa aaagagcaat ccatgaagca cattctcggc
     4861 catcaaacag atgatcatcc ttatatcgag taaatggtac acctacttta cgcatacgtt
     4921 cttcaatagg ttccatttca cttcttgttc gagcaagaat ggcaaaatcc ttaaacgtga
     4981 cgtctcgcca ttccttgcat ttttctttat tgaaaacttg cagacgagtg tgcagaccat
     5041 tcgaagtttt tacataagag caacattcta taatttttcg aacacatgtt tcagcgaagg
     5101 attcctctga aagagactct tctgatatcc agaatggcac ggcgtttgtc cattctttaa
     5161 gattttcatt ccaaaaacgt gctgcgtttt tttgttgttc tacgctttcg ggcggctctg
     5221 atttatggaa cgaagaattg tcaccaaaga aacttttgca agagaaaaat cgattgcagg
     5281 cttcaatcat ctcattagtc gaacggtaat tgttattgag ttctttggaa ttattaattt
     5341 ctttttttgc actacgatag acatttacat cggcaccctg aaaactataa atggattgtt
     5401 tcggatcacc taccacgaga acggaatgct tatcgcatat aaatattttt ttgaaaatat
     5461 cccactgtaa ttgatttgta tcttggaact cgtcgatgat ggcatgagta tattcggagc
     5521 gcagtttttt acaaagattg ctatcacttc catcaagtga cttttccata acggcacgat
     5581 ggactgaaag aatcatgtcg ttgaaagatt gagacttatg atccttctta atgcgttgcc
     5641 attcctcaaa taataaagga atttgagagt agataaactt ttgtttgact gcgtcgggat
     5701 atttggatat ctcttctttt aatttgtaaa agaacttaaa agcgccgtat acttcggatg
     5761 gccaactttt tggagttttt ccatcactaa aatctccagc aatgtaaagt tctgaacttg
     5821 ttccttgcca gcgttttaaa gctgcagcaa agtcgcctaa tgtttttgat tcattatcct
     5881 tattaaaagt acacccacta ttagcctcaa taatatcata atttttcttt acttctggaa
     5941 ccaataataa gtcatcaaat ttttctggcc attttggaaa attcggaata actgcagaat
     6001 ctatttctac aatttcttca ccatctgcag acttaccata ataaaggggt atggcattct
     6061 tcaattcttt tttcaaatca cttacatttg tgtcatgttg aatcagcatt ttatacagcg
     6121 gatcatgagg ccatttgtct cttgcccatt catcaatgac gtctaatact tcgttatcgt
     6181 caatcatatt taagtcgaat ggccgccctg catcataagc gaattctttc agtgcttttt
     6241 ggcaaaaaga atggatggta aaaatggagg cgttatccac ttcacgtaag gccgtttcaa
     6301 aaacatctaa atcgtcttgt gaattatttt gggaatctaa aacctcttcc agttttttac
     6361 gaatgcgatc cttcaattct ccagctgctt tttcagtata ggtgacaatt agaattttct
     6421 taagagagat accttctttt acaaggcgag ctacaatctg ctgaatagta tacgtttttc
     6481 ctgtccctgc agaagcctcc acaacccaat tttcttctag gtcaatctta tcaataaaac
     6541 tcatttcgtg ttctcctcac catcgatcga aaagcacatc agatttctct gttgtttggt
     6601 agccttttcc cattcttctt caaaattatt gggagagaat cctaagtctt ttcgcgtatc
     6661 aaaatttttt gcttttttga aatatttcca aaggttatga tcaccagtaa actttttagt
     6721 gtaatcactg aatgtttcaa ttgtttcatc cactatatcc gcaggaaccg ccttagaata
     6781 tttttcgcag aaggcttttt gataaagttt cttcaatatt tctattgctt tattcggaga
     6841 taggacaaca ttagccttcg caggcttttt aatgctagaa gctccactat atatttgaat
     6901 ttcaatatga cgttcgtcat cgtattttga tgtttcactc gcgataatag caagtgcagc
     6961 aacataagat gataaatatt tacccttatc aaaatagggg cttgtttttg cagcatgtgg
     7021 atctgatgta gtcaaataca atagacaatt tcttttcttg ttgcaccaac caagagatcc
     7081 ttgtaaagtc cacttggttc catcaagttg ctccatcggc aattcaatac gttcgtcaaa
     7141 tgtccagtct gtggaattcg atttcggtga attttcatct atttccatta gttttatgat
     7201 gttttcagca tacttttcag catcttgcca cattttttca ccataaacac catcgggaat
     7261 gtctccaata gaaagcatct gccatttcag cttttcaagc gcttcgtgat cgcccgtgat
     7321 tttaaattta accatctgtt tcatcaaaga acttttatct agtatatcaa acacaatagg
     7381 ttcgaaatct tcatcagtcg ggtcatcgtc ttcttcacca tcgtttagca cctgagaaac
     7441 acgaaactgg aatggatctt caagatattt tttaaactga taaaaagaaa cgcggtctgg
     7501 tgggagctct ttggatagtg ccaaaggctc tttttctaca tccgattcgt cacctgactt
     7561 tttcatatct tcgaaaatgg acttgtttcg aaattccttt tgcgtaaaca gttcaccgta
     7621 atcacgcgat tcgtcgagtg gaatcacttc ttgcggccag actttcttag cattgacatc
     7681 tattccgttt ttacgaagtg catttttcaa aaaatttcga atatcggcca caacagaaga
     7741 cgggtacaaa tcttcatctt tgacaatatt tttattcaag taactgatat ggagcgattt
     7801 tttcacgctc atcagctggc aaagaaaagc atagcgttgt ttggcaacag gagaatcatc
     7861 accaggccac ggcgctacgg acctccgcag atcaagcgta ttctggcttt tactcccagg
     7921 aaaggttcca gaatcaagtc caataaagaa caagtgcttt actggaataa ttctattcgg
     7981 aacaaatttt gcaaaagtaa tgccgttcat aaataagctt ccacaactat actcagaagc
     8041 ttctgcagca gaaataagcg tttgcgaaat acaatcccat gaaacttctg tagcacctgc
     8101 atcaaactgg aatttcaaat cttcaagaga attcaaaacg ttctggaaaa taatcgtttc
     8161 cccgttcatt ccatcgggcg gattcgtcat cgctagccac ttgttgagca tcgtggagat
     8221 taaatccaac tcccctagct ctaccgtttc tttgttacgg gcaaactcaa tccattcttc
     8281 aagttctgtg acgcagtccg caaaacggct taaactagca tcattttcgc tattgatatc
     8341 catgtaagga gcatagcctt cgctagtctc gtcacctgcc gcactaattt tatggtcgct
     8401 gaatcttgaa gccattaatc gttttaatcc aaaagtccag tcattttttc catttccacg
     8461 cgtgcggaaa acattcatat tttcaaccca agattcccaa gcgcttacat cttcattgct
     8521 gatttgccgt gtctgttgta ttactggatt acgcacaaga tcaaagaaaa tcgaacgaga
     8581 gagagtgcgt ttttcctgca tcgcaaaaag ggcggaaaga gcttcgcctg ttaaagattt
     8641 cttggctggg gaatctacaa tagagaacgg aatttgaaac gactgctgat caaagcactg
     8701 gaaaatcgct gtgcgataaa catcaagatt gggagagact accagaatat cgctagttcg
     8761 atttacggga ttgccatctt tatcgcgttt cgcaaggagt ttacagattc gggtatggag
     8821 agcctcgatt tcacgttcac gcgttggagc agcggtcaat gaaaaacttt cattatcttc
     8881 actcaacaaa ccaggcagct catttgtacg attggccacc atatattgaa tctggtgaag
     8941 caatgaatcg ctcgggagtt ctaacgattc ttcttcaaat tcaaaattgt aatcgttcgc
     9001 ttggcaccac aacttgatat tatcgcgtcc agattttccc caatagctaa gaagggcgtt
     9061 ttcatttcgc gagttcacgc cttcttcata atcatcattc acttcaacac cagctgattc
     9121 attgagacta taatgcaact tttgccttac tgtacttgga atctctccgt tttcactctg
     9181 ccacacaccg tttctcgcgt tccactggcg atggcgtgat tttttagcaa cattggtgtc
     9241 ttcccaaaat tccatgcaag ggttttgaat gtaagcattc acatcatgcg ttttagcata
     9301 ctcctgcaaa ataacgcggt agaattgccc catccctgta aggccgaaaa taaacactgg
     9361 aagcgttttt tcatttatcg aaaactgttc tgtatgaaat ttgccatgat tcagcataca
     9421 atcttggaac aaaaacggaa tcgtcaggta agtggtttca aacttaatct ttttgccttt
     9481 atttatacgc tgattataat tttcaaatgc cttcgtgaga atcgaaggtt cgctaccctg
     9541 cgtatgaaaa atctgagaat aaagttttct ctgccatact tcgcgttcac ttccatcaaa
     9601 aaaattctgt agattcccct gtttccacct atctagaatc ccttccgcca aatccccttt
     9661 ttcgttcaga ttacttcctt tatgaacaaa tcctttaggg cggctcgttt cgtattccag
     9721 aaaaagcgtc gccattttac tgcaaaggtc aaacaagtgc ttttcatcta gtttgccctc
     9781 cacttcaagg tagcgtgtga cttcatcgcc aagagactta taatttttgt aaaggtacgc
     9841 ccagattacg cttcgtaaaa gatcggcgtt tagtttcttc tttgtgttat catttgggca
     9901 aagaatcgtc atcaaaaaac gatcgatcat catggagtta aaattcgcaa gagtgccctg
     9961 cgtttttacc caatgcaagc gaaaccaata ctccaatttt ggatctggaa aaataacaat
    10021 tggagcgacg agcggatttg tccacgagag cttaattttc gagatcattt catcggcaag
    10081 tttttcaata ttcaaagcaa actttaagta taacatgaca cccttttttt atgcattttc
    10141 aatctaaata taatgtctca aaatgacaaa aaatgacaaa atgagctttt tatagaaata
    10201 aaagtagaaa accagagtaa aagacgcatt gaatcatata ttccttcttt ggatttgtag
    10261 tttttttaat agtgggttca tgaacgacca tattcattac cgg
//
LOCUS       DAOOBB010000054        10195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_8229_length_10195_cov_5.262229, whole genome shotgun sequence.
ACCESSION   DAOOBB010000054 DAOOBB010000000
VERSION     DAOOBB010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 10195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10195
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8229_length_10195_cov_5.262229"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="PLT31_03945"
     CDS             <1..566
                     /locus_tag="PLT31_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94321.1"
                     /translation="SPDVAAGGVATWTVSGCLTTANISGYAWAGSPAVPTTTFAHTFT
                     AKGETFTPTLSVYNDDNTVQPVTCPAATAIDATLPDYEMTASGNTAGITFEGNVDATI
                     VMNLPETWHGETAGTCSFACQVNRGASGSGASGSGAISGTVDEVDITGSDYVTALIPI
                     TSTINGYALKLVLDVGTNAGVTCFLNW"
     gene            652..3015
                     /gene="sppA"
                     /locus_tag="PLT31_03950"
     CDS             652..3015
                     /gene="sppA"
                     /locus_tag="PLT31_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HPW94322.1"
                     /translation="MILVFHKKIISLLVRPILRKGLFGFSKNLFGFFFVLLGIPTFLQ
                     AYIPGESFVSLTDDQGLFSNPAGLSAFDSKGGLLSYEYSQEKIHQLQLGFNLGAFGFS
                     FDYQTDQKGFDESRWHLTNAFPLFDRSVFFGHRLTAFRSGDFDGTAVIYSPGLLWRPV
                     SFLSLGFTSYNLLQMSSDNLDRIQEAGAALRLGRKLTLGYEIENLESHQLFLQTSLSG
                     VEIGFQIPIYGDKKEYKLSLSYPLDAYMNAAVSVFDDFIPRRVAFSYHRSRNPKAYAR
                     SLIIRVPLSLTVKETEERFAFFKDPSMSVWAVRNHFEQILHDPSTGIIIFDFSGYTGG
                     AAISKEIQRGISTLRESGRKVVAYLDDVRPTVLLASASADRIVIEPSARVNLRGLAGT
                     SLYYKGFLDWIGVDVQFLRHGDYKAAVEPYTMDSMSVEARSDRESLYNEWWSVLVEDF
                     QKRLPAGLQVDDFVKDPLLTAKSAKARQWVDTILYIDQVPAYALKEFFALDIPQAKAV
                     SWAPTDRKILADDWTPRSKVAVLNIEGTIDKKTEVESIVLIDEVMQEGKYEALIVRIN
                     SPGGDAQASEKIWRALRVLSESGIPVVASIGDMAASGGYYIACGADQIIAEKSSIVGS
                     IGIFGGKVNASALLEKLKLRAETVKTHEHADAESFTRSFSEVEKKALQEYMDDFYLRF
                     TKVVSDATKIPQEKVDQEYGGGRVFVGKKAMSLGLVHSLGGLDDAISAVKKMAKISVS
                     KQVEIVPLVSKSSYINRVSFKSLAEFVSSFDGDFIWALESDLWPLLQ"
     gene            2997..4046
                     /locus_tag="PLT31_03955"
     CDS             2997..4046
                     /locus_tag="PLT31_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPW94323.1"
                     /translation="MAAIAITIFLTLLLSAFCSITEASFYSVSNTVVESLQRQKKFTG
                     KYIAHVKKNIDTYIASVLILNTLTNTIGVYMTTKLAVSTLDSVGQMILPIVLTVSILL
                     FGEITPKTVGVKKANKVGPIVAIPFYYITIVLKWTGLIWVCIALTKRWTKHEKKNDVS
                     IEDINSLVTLGLREEILDRQQASVIKNILALKSICVRKIMTPRQVVFSLSGDLTIGQS
                     IDKRGNWPFSRVPIYSEKKDQWIGIVLRRDVYNKLVEGDHQLQLKQLMRPIQLIPDSL
                     TLDKLLLRFLKQRGHIVGVVDEWGAIAGIATLEDVLEEILGREIVDEYDETIDLQKQA
                     RRKSKALAFIRKQEK"
     gene            4370..4804
                     /gene="rplM"
                     /locus_tag="PLT31_03960"
     CDS             4370..4804
                     /gene="rplM"
                     /locus_tag="PLT31_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPW94324.1"
                     /translation="MKTIMVNPKTVTHKWKLVDAAEKPMGRVASEVARLLMGKHKAIY
                     SPNVDTGDFVIVINAEKVMLSGNKIDQKEYFHHTGHIGGERWINCKDLMVKHPTAPLE
                     MAIWGMLPHTNLGRKMMKKLKIYAGAEHQHAAQNPETVDLKV"
     gene            4822..5226
                     /gene="rpsI"
                     /locus_tag="PLT31_03965"
     CDS             4822..5226
                     /gene="rpsI"
                     /locus_tag="PLT31_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPW94325.1"
                     /translation="MATAKAKKIYRGTGRRKNAIAAVVLRPGSGKRTINGRDFKEYFH
                     SDVQNMIANLPFAILENEGEWDVEVTARGGGIAGQMGAVRLGIARALVANDAEVKPAL
                     KKEGLMTRDARAVERKKFGRKKARKRFQFSKR"
     gene            5798..6532
                     /gene="rpsB"
                     /locus_tag="PLT31_03970"
     CDS             5798..6532
                     /gene="rpsB"
                     /locus_tag="PLT31_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPW94326.1"
                     /translation="MANLPSVEDLLAAGAHFGHQTQRWNPKMKPYILAEKNGIYVLNL
                     SKTRDLLDAAAKAAARVSASGKSVLFVGTKPTARQVVAEAAGTCNHFSVTNRWLGGML
                     TNFQTVRKSIKQIDKIDAMEKDGLFQELSKKEVLDKTRERDKLLSVFGGIREMGGIPG
                     LVVVTDINHEKIAVAEARRLHIPIIGICDTNVDPTLVDYPIPANDDAVKSVKLIIDYI
                     ASNVTPRSNVAKEANEAAKKFDNGEN"
     gene            6534..7427
                     /gene="tsf"
                     /locus_tag="PLT31_03975"
     CDS             6534..7427
                     /gene="tsf"
                     /locus_tag="PLT31_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HPW94327.1"
                     /translation="MQITASLVNELRTKTGVGMMQCKKALTETDGDLEKALDLLRKQG
                     AAVAAKRADKDAKEGRVFLVEADSRAVAFELSCETEPVSKNEDFNDLANLATKVLSTQ
                     EINSVESLLEASADGVKVGDRLQDVLVKIQENISFRKLATMKRSASEVFGIYSHMMGK
                     IGVITKLAYVGTPSNEADLKQVAKEISMQAAAFAPIAVKAEDVPAETIEKEREIARAQ
                     IENAAKESGKVTKPEFVERQLDGRVAKVLKEIVLEDQEFFISDKNPKKLNVKEYLQSI
                     ASSLGLSSLQVVELIRFERGN"
     gene            7427..8143
                     /gene="pyrH"
                     /locus_tag="PLT31_03980"
     CDS             7427..8143
                     /gene="pyrH"
                     /locus_tag="PLT31_03980"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HPW94328.1"
                     /translation="MAHAPRFSRVLLKLSGEALAGTKGHGIDSEILTTMAREIAAVLK
                     SGVQVALVIGGGNLVRGISASAGGMNRAAGDAMGMLGTVMNGIAMQDALDKQGVNSVV
                     MSAIRMEPVCEFFDRRKAISLLNSGSVVIFSAGTGNPFFTTDSAAALRAIETECDVIM
                     KATKVDGIYSADPMKDPNATRFESITYSEVIQKGLQVMDTAAVALCMEQKMPILVFQM
                     IEGNLTKATTEGTLGTLVHC"
     gene            8178..8711
                     /gene="frr"
                     /locus_tag="PLT31_03985"
     CDS             8178..8711
                     /gene="frr"
                     /locus_tag="PLT31_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HPW94329.1"
                     /translation="MSEYVQKMEKAVEATEREFSKLRAGQASPAILNDVRVDYYGTPT
                     PISQVAKISVPEPRMLLVSPWEKPLMDAVEKAILAANIGVTPMKDGNCIRVALPILTT
                     ERRQELAKIARKHAEDGRVAIRNIRRDANDAIKKQKDLPEDEVKKQQDEIQKATDKYI
                     GMIDRVLQEKENDILKV"
     gene            8714..9424
                     /locus_tag="PLT31_03990"
     CDS             8714..9424
                     /locus_tag="PLT31_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HPW94330.1"
                     /translation="MENQLRHVAIIMDGNGRWAKRQGFERFFGHRKGTQATIDAVEVG
                     VDLKLEHLTLYVFSSENWGRPSKEVDYLMKLLVEMVEQELPHLMEKQVKLIVIGNMDR
                     LPKEPRSKLQEAINKTANNTGMQLNLAISYGGRLEIIDSCKKIASKVKNGSLNIEDIN
                     EEVFSNHLYLEGAPDPDLVIRTGGEFRLSNYLLWQAAYSEFFVSKTLWPDFTKEEFLE
                     AVAFYQTRERRFGKVVSE"
     gene            9417..>10195
                     /locus_tag="PLT31_03995"
     CDS             9417..>10195
                     /locus_tag="PLT31_03995"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPW94331.1"
                     /translation="MSNLAQRLIFAAVAIPVVFFCLWWADWTRISLMVFLGAVGAWEW
                     SRMVSKIYSGPSMQIFAPLSAAALTLGWVFTSGRFFGLPPVPGLIGFIALIVVCLYIA
                     IAFAKVKIDNLFPWFLMQLGAPMYLGLWGGLATLILGSGHGLEHSYKFILVMTAMWGC
                     DTIAYFVGKFLTNRFIFGRHLMAPQISPKKTWEGAIGGTLFTMGWVAFWAIPVFQYPW
                     YKGLVLGLVLAIAGQAGDLLMSALKRWSGTKDASQIFVGHGG"
BASE COUNT         2969 a         1783 c         2189 g         3254 t
ORIGIN      
        1 cttctccaga tgtggctgct ggtggtgttg ctacttggac agtgagcggt tgtttgacaa
       61 ctgcaaatat ttctggttat gcttgggctg gttctccagc tgttccaacc acaacatttg
      121 ctcatacctt cacagcaaag ggagaaactt ttactccaac cctcagtgtt tacaatgatg
      181 ataatacagt tcaacctgtg acttgtccag cggcaactgc tattgatgcg actctgcctg
      241 attatgaaat gactgcttct ggaaatactg ctggaataac ttttgaaggg aatgtggatg
      301 ctactattgt tatgaatctc cctgaaacat ggcatggcga aaccgctggg acttgttctt
      361 ttgcttgtca agtgaataga ggtgctagtg gtagcggtgc tagtggtagc ggtgccatat
      421 ctggaactgt agatgaagtt gacattacag ggtctgatta tgttacggca ttgattccta
      481 taaccagtac gataaacggt tatgccttga aattagttct tgatgttgga acaaatgctg
      541 gtgttacttg ttttttaaat tggtaaaatt agtttagcat agtttttata aaatcacctc
      601 ttttgtagag gtgttttttt ctttatagaa aagggttttt atatttaaat gatgattctg
      661 gtatttcata aaaaaataat ttctctttta gttcgtccta ttttacgaaa aggtcttttt
      721 ggcttttcaa aaaacctctt tggatttttc tttgttcttt tgggaatacc tacttttttg
      781 caggcttaca tccctgggga atcatttgtc tctttgacag acgatcaagg actattttca
      841 aatccagcag ggttatctgc ttttgattct aaaggagggc tactctccta tgaatatagt
      901 caggaaaaaa ttcatcaatt acaattaggt tttaatttag gcgcttttgg cttttctttt
      961 gactatcaaa cggaccaaaa gggttttgat gaatctcgtt ggcacctcac aaatgcgttt
     1021 cctttatttg atcgatccgt cttttttggt catagactca ctgcttttcg aagtggcgat
     1081 tttgatggaa ctgcagtgat ttattctcct ggtcttttgt ggcggccagt ctcatttctc
     1141 tctcttggtt ttacaagtta taatcttctt caaatgagct cagataacct agaccgtatc
     1201 caagaagctg gagcggcact ccgattaggg agaaaattga ctttaggtta cgaaattgaa
     1261 aatttggaat ctcatcagct gtttttacaa acttcattga gtggagtaga aattggtttt
     1321 caaattccaa tttatggaga taaaaaagaa tataaacttt cgctctctta ccctctcgac
     1381 gcctatatga atgctgctgt ctcagtcttt gatgatttta ttcctcgtcg ggtggccttc
     1441 tcttatcacc gttcgagaaa ccccaaagcg tacgctcgtt ctctaatcat tcgtgttcct
     1501 ctaagtctta cagtgaaaga aacggaagaa cgattcgctt tctttaaaga cccttcgatg
     1561 agtgtttggg cagtgagaaa tcattttgaa caaatccttc atgatccttc gacgggaatc
     1621 attatttttg atttttcagg gtatactggt ggtgctgcaa tttcaaaaga aattcagcgt
     1681 ggtatttcta cgttacggga gtctggcaga aaagtagttg cttatttaga tgatgttcgt
     1741 ccaactgttt tacttgcgag tgcttctgct gatcgcattg tcattgaacc ttcggctcga
     1801 gttaatttaa gaggccttgc tggaacctct ctttattaca aaggcttttt agattggatt
     1861 ggagtagacg tacaattctt aagacatgga gattacaagg ctgccgtaga gccttatacg
     1921 atggattcta tgtctgttga agctcgttca gatagagaat ctctttataa tgaatggtgg
     1981 tctgttttgg tagaagattt tcaaaaaaga cttcctgctg ggcttcaagt tgatgatttt
     2041 gtgaaagatc ctttattgac agccaagtcg gccaaagctc gtcaatgggt agatactatt
     2101 ctttatattg atcaggtgcc tgcttatgca ttaaaagaat tctttgcttt ggatattcct
     2161 caagcaaaag cggtatcttg ggctcccaca gacagaaaaa tactcgctga tgattggact
     2221 cctcgttcga aggtagctgt tttgaatatc gaaggaacga ttgataaaaa aacagaagtg
     2281 gagtctatcg tcctaataga tgaagtgatg caagagggta aatacgaagc attgattgtt
     2341 cgaattaact cgcctggagg cgatgctcaa gcatcagaaa aaatatggcg tgctttaagg
     2401 gttttatctg aatctggaat tcctgtcgtc gcaagtattg gagatatggc tgcttctgga
     2461 ggctattata tcgcctgtgg agcagatcaa atcattgccg aaaaatcctc aatcgttgga
     2521 agtatcggta tctttggcgg taaagtgaat gcttctgctt tgcttgaaaa attaaaatta
     2581 cgtgcagaga ccgtaaaaac acatgagcat gctgatgctg aaagctttac acgtagcttt
     2641 tcagaggtag aaaaaaaagc gttacaagaa tatatggatg acttctatct tcgttttacg
     2701 aaagtggtct cggatgccac aaaaattccg caagaaaaag tggatcaaga atatggtggc
     2761 gggcgagtct ttgtcggaaa aaaagcgatg tctcttggct tagttcattc tcttggtgga
     2821 ttagatgatg cgattagtgc ggtgaaaaaa atggcgaaaa ttagcgtttc taaacaagtg
     2881 gaaatagtgc cactagtttc taagagctct tatattaatc gtgtttcatt taaaagtctt
     2941 gctgagtttg tttcttcatt tgatggagat tttatttggg cattggaatc tgatttatgg
     3001 ccgctattgc aataactatt tttcttactt tattactctc tgccttttgt tctattacag
     3061 aagcatcttt ttatagtgtt tcaaatactg ttgtagaatc tttgcaaagg caaaagaaat
     3121 ttacaggcaa atacatcgct catgtaaaaa agaatattga tacctatatt gcttctgtat
     3181 taatcttaaa tacgttaacc aatacgatag gtgtttatat gacaactaag cttgctgtct
     3241 ctacgcttga ttcagttggc caaatgattt tgcctattgt attaacggtc tctatattat
     3301 tatttggtga aatcactcct aaaacagttg gtgttaaaaa agcaaataaa gtagggccta
     3361 ttgttgcgat acctttttat tatatcacta tagtattaaa atggactggc ttaatatggg
     3421 tttgtattgc tttaacaaag cgatggacta agcacgagaa aaaaaacgat gttagtattg
     3481 aagatattaa tagcttagtg actttaggtc ttagggaaga aattttggat cgtcagcaag
     3541 cttctgttat taaaaatatt ttagccttaa aatcaatttg tgtcagaaaa atcatgactc
     3601 caagacaagt cgtattttct ctctcaggag acttgacaat agggcagtct attgataaac
     3661 ggggtaactg gcctttttct agagtgccta tttattcaga aaaaaaagac caatggattg
     3721 gaatcgtttt acgccgagat gtctataata aattagtcga aggagatcat caattacaat
     3781 taaagcaatt aatgcgtccc attcaactca ttccagatag tttgacttta gataagttac
     3841 tgcttcgttt tctaaaacaa cgtgggcata ttgtaggcgt tgtggatgag tggggggcaa
     3901 ttgcagggat tgctacactc gaagatgtcc tagaagaaat tcttggccga gaaattgtgg
     3961 atgaatatga tgaaactatt gatttgcaaa aacaagcaag aagaaagagt aaggctttag
     4021 cttttattcg caaacaagaa aaatagttgg ctcgaatttt cctttcaaat attaaaagag
     4081 tcattgaaat agcttagtaa atagataaaa catttataaa atgttgagtt atataacttt
     4141 gtatacccaa atacatttgg gcaggagtta tacatcatag gtatacaatg atgaaatata
     4201 atgctataat tatagcaatt gatataattg atatgatgta aaaccttgat ttatagagga
     4261 tttctttaaa aaagagtttt ttgactcttg tttttggctt ctcttattct aaattttgcc
     4321 cgaactattc agaacaattc gttttgatta aacctaatcg aggatttaca tgaagaccat
     4381 tatggtaaac cccaagacag tgactcacaa gtggaaactt gttgatgctg cggaaaagcc
     4441 tatgggccgc gtagcaagtg aagtggctcg tcttcttatg ggcaaacaca aggcaattta
     4501 ctctccaaac gtagatactg gtgatttcgt cattgttatt aatgcagaaa aagtaatgct
     4561 tagtggaaat aaaatcgatc aaaaagaata tttccatcac acaggccaca tcggtggcga
     4621 acgttggatc aattgcaaag atcttatggt aaaacatcct acggctcctc tcgaaatggc
     4681 tatctggggc atgcttcctc ataccaatct tggccgcaag atgatgaaga aattaaaaat
     4741 ctatgctggt gctgaacatc aacatgcagc tcaaaaccct gaaaccgtcg acttgaaagt
     4801 ctaggaacgg aggataaatc tatcgctact gcaaaagcaa aaaagatcta ccgtggcaca
     4861 ggtcgtcgta agaacgccat cgccgctgtt gttttaagac cgggttccgg taagcgcacg
     4921 atcaatggtc gtgatttcaa agaatatttc cattccgacg tgcaaaacat gatcgcgaat
     4981 ttaccatttg ccattcttga aaatgaagga gaatgggacg tcgaagttac cgctcgtggc
     5041 ggtggaatcg ctggtcaaat gggtgctgtt cgtctaggta tcgctcgcgc tctcgtcgct
     5101 aacgatgccg aagtcaagcc tgctttgaaa aaagaaggtt taatgactcg cgatgcacgt
     5161 gctgttgaac gtaagaagtt tggccgtaaa aaggctcgta aacgcttcca gttctccaaa
     5221 cgttaatcgt ttacaacgta tttcaaaaaa acgccttcta tgcaaggcgt ttttttgtgt
     5281 atttagagcc accagctagg tactccgttt gccctcatga ctgaaaatat cacaacgcaa
     5341 accattattt ttgctgccga taattgctta aaaagtttta ttctttggtt aataaccgta
     5401 aagtagcgaa aagtgtatag gttccttaca aaacgatact tactttattt gtaattcagc
     5461 aaatatcttg tcaatagttc gttccaaaaa cttactttca ttatttgtct tggatgctta
     5521 cttaaaaagt tataaggacg cttttttagt aataacagct tagcataagt tttcttaaaa
     5581 tagtgattca aaatactacg ttttctttca tttccgattt gatttctcta gtcacttcat
     5641 ttgatttttc tatatttggc tccaacttgg atattaagtc cgagtcaacc aataaatcac
     5701 cggtgaacaa aagttgcttc ggtggcgaaa aaagaaactt tttttgctta tgcatcttag
     5761 ttgtttaccg ggtagtttaa ccgattagga gtcaattatg gcaaatctgc cttccgtaga
     5821 agacctgctt gctgcaggcg ctcattttgg tcatcagaca cagcgctgga acccaaaaat
     5881 gaaaccttat attcttgccg aaaaaaacgg catctatgtt cttaaccttt caaagacccg
     5941 tgatctttta gatgctgctg caaaagctgc tgcccgtgtc tctgcttctg gaaagagcgt
     6001 tctttttgta ggtacaaagc caactgctcg tcaagtcgta gcagaagctg ctggtacttg
     6061 taatcacttt agcgtaacca accgttggct cggtggtatg ctcacaaact tccagactgt
     6121 tcgtaagtca atcaagcaaa tcgataagat tgatgctatg gaaaaagacg gtctattcca
     6181 agaattatca aaaaaagaag ttttagataa gactcgtgaa cgcgataaat tattatccgt
     6241 gttcggtggt atccgtgaaa tgggcggcat tccaggctta gttgttgtca ctgacatcaa
     6301 ccacgaaaaa attgctgttg ctgaagctcg tcgtttgcat attcctatca ttggcatttg
     6361 cgatacaaac gttgatccta ctctagtgga ctatccaatt cctgcaaatg atgatgcagt
     6421 gaagtctgta aaacttatta ttgattatat cgcaagcaat gtcactcctc gttctaacgt
     6481 agctaaagaa gcaaacgaag cagcgaagaa gtttgacaat ggagaaaact aaaatgcaaa
     6541 ttactgcctc tttagtaaat gaacttcgca ccaagactgg tgtggggatg atgcagtgca
     6601 aaaaagcgtt gactgaaacg gatggcgatt tagaaaaagc tcttgattta cttcgtaaac
     6661 aaggtgcagc tgttgccgct aaacgtgcag acaaagatgc aaaagaaggt cgtgtatttc
     6721 tcgttgaagc agattctcgt gctgttgctt ttgaattgag ctgcgaaaca gaacctgttt
     6781 ctaaaaatga agattttaac gatcttgcta atcttgctac aaaagtttta agtacgcaag
     6841 aaatcaattc tgttgaaagt ttacttgaag cttctgctga tggtgtaaaa gtgggcgatc
     6901 gtcttcaaga tgtgctcgtt aaaattcaag agaacattag cttccgtaag ttagctacaa
     6961 tgaaacgttc tgctagcgaa gtgtttggca tttattctca catgatggga aaaataggcg
     7021 taatcactaa gcttgcttat gttggtactc cttctaatga agcagacctc aagcaagtcg
     7081 ctaaagaaat ttcaatgcaa gctgctgcat tcgctcctat tgctgtaaaa gcagaggatg
     7141 ttcctgctga aactattgaa aaggaacgcg aaattgctcg tgctcaaatt gaaaacgctg
     7201 ctaaggaatc tggaaaggtg acaaagcctg agttcgttga acgtcaatta gacggtcgtg
     7261 tagctaaggt tttaaaagaa atcgttcttg aagatcaaga atttttcatc tccgacaaaa
     7321 atccaaagaa attgaacgtt aaagaatatc tacaaagtat tgcttcttct cttgggctct
     7381 cgagcttgca agtcgtagaa ttaattcgct ttgaaagagg taactaatgg ctcacgcacc
     7441 aagattttct agagtgctac tcaagttgag tggagaagct cttgctggta ccaaaggaca
     7501 tggtattgat tccgaaattt taacgacgat ggctcgagaa attgctgctg tcttaaaatc
     7561 aggtgttcaa gtcgctcttg ttattggtgg tggtaatctg gtgcgtggaa tttctgcatc
     7621 tgctggcggc atgaatcgtg ctgctggtga tgctatgggt atgttaggta cagtcatgaa
     7681 tgggatagcc atgcaggatg cgttagacaa acaaggcgtg aatagcgttg tcatgtctgc
     7741 gattcgcatg gagcctgttt gtgaattttt tgatcgccgt aaagcgatct ctttattgaa
     7801 ttcggggagt gtggttatat tctctgcagg tactggaaat ccgtttttta ctaccgatag
     7861 tgctgcagct cttcgtgcta ttgaaactga atgtgatgtg atcatgaaag cgactaaggt
     7921 cgatggtatt tattctgcag atccaatgaa agaccctaat gcgactcgtt ttgaatcgat
     7981 tacctattct gaggtaatcc aaaaagggct tcaagtcatg gatacagccg ccgttgcttt
     8041 atgtatggaa caaaaaatgc ctattttagt gttccaaatg attgaaggaa acttgactaa
     8101 agcgaccact gaaggtactt taggtacact agtgcattgt taatttttat tattgcacaa
     8161 gaaagaggaa aataaatatg tcagaatatg tgcaaaaaat ggaaaaagcg gtcgaagcga
     8221 ccgaacgtga attttcaaaa ttaagagcag ggcaagcttc tcctgctatt ctaaatgatg
     8281 ttagagtgga ttattatgga actcccactc ctatttctca agtggcaaaa atttcagttc
     8341 ctgaacctcg catgttatta gtgagccctt gggaaaagcc tttaatggac gctgttgaaa
     8401 aagccattct tgctgctaat attggtgtta ctccaatgaa agatggtaac tgtattcgtg
     8461 tggctctccc tattttaact acagaacgtc gtcaagaact cgctaaaatt gctcgtaagc
     8521 atgctgaaga cggacgcgtg gctatccgta atattcgtcg tgatgctaac gatgctatca
     8581 aaaaacaaaa agatcttcct gaagatgaag tcaaaaaaca acaagacgag attcagaaag
     8641 cgaccgataa atacattggt atgatcgatc gtgtgctcca ggaaaaggaa aacgacattc
     8701 ttaaggtttg agtgtggaaa atcaactcag acatgtcgcc atcatcatgg atggtaatgg
     8761 aagatgggcc aaacgtcaag gttttgagcg tttctttgga catcgaaagg gcactcaggc
     8821 tactattgat gcggtagaag ttggtgtcga tttaaaatta gaacatttaa cgctatatgt
     8881 tttcagttcc gagaattggg gccgaccttc aaaagaggtt gattatttga tgaaacttct
     8941 tgtagaaatg gtagaacaag aacttcctca tttaatggaa aaacaagtga aattaatagt
     9001 cattggtaat atggaccgat taccaaaaga acctcgttct aaattacagg aggctattaa
     9061 taaaacagcg aataatacag gcatgcaatt aaatcttgcg atttcttatg gaggtcgttt
     9121 agaaataatt gattcttgta aaaagattgc gtcaaaagtt aaaaacggat ctttgaatat
     9181 tgaagatatt aacgaagaag ttttttcaaa ccatctttat ttagaaggcg ctcctgatcc
     9241 agatcttgtg attcgtacag gtggagaatt tcgtttatcg aactacctac tttggcaggc
     9301 cgcttacagt gaattttttg tttcaaaaac gctttggcct gatttcacaa aagaggaatt
     9361 tcttgaggct gttgcgtttt atcaaactag agaacgtcgt tttgggaagg tagttagtga
     9421 gtaatctagc acagcgttta atttttgctg cagtagcaat tcctgtggtc tttttttgtc
     9481 tttggtgggc agattggact cgaatttctt taatggtttt tcttggtgct gttggtgctt
     9541 gggaatggtc tagaatggtt tccaaaatat actcggggcc aagtatgcag atttttgctc
     9601 ctttatctgc tgcggcactt acactaggat gggtttttac ttctggtcgt ttttttggac
     9661 taccacctgt tcctggatta atcggtttca ttgctctaat tgtcgtttgc ttatacattg
     9721 ccatcgcttt tgcaaaagta aaaattgata atttattccc ttggttttta atgcaattag
     9781 gcgctcctat gtacttaggt ctttggggtg gattagccac tttaatttta ggttcagggc
     9841 atggcctaga acattcatac aagtttattc ttgtgatgac agccatgtgg ggctgtgata
     9901 ccattgctta ctttgtaggg aagtttttaa ccaatcgttt catctttggc cgtcatctaa
     9961 tggctcctca aatcagtcct aaaaaaacat gggaaggtgc aattggtggc acattgttta
    10021 ctatgggttg ggttgctttt tgggctattc cagtttttca atatccctgg tacaaaggtc
    10081 ttgttcttgg tttagtttta gcgattgcag ggcaagctgg tgatctttta atgtctgctc
    10141 ttaaacgttg gagtggtaca aaagatgcaa gccaaatttt tgtagggcat ggtgg
//
LOCUS       DAOOBB010000055        10080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_8347_length_10080_cov_5.341845, whole genome shotgun sequence.
ACCESSION   DAOOBB010000055 DAOOBB010000000
VERSION     DAOOBB010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 10080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10080
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8347_length_10080_cov_5.341845"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="PLT31_04000"
     CDS             complement(<1..635)
                     /locus_tag="PLT31_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPW94332.1"
                     /translation="MQNELILKAADNVRILSAAMVQKAKSGHPGGAMGAADAITLLFA
                     EFLRFNPDQPSWMGRDRFFMDPGHMSPLLYSELALTGFMGLSDLAEFRQLNSETPGHP
                     ELAVHRAIENSSGPLGLGHGMALGTAIAERFMVERFGDIVSHKTVVLISDGGVQEEIA
                     YGVGRVAGHLQLSNLILFYDANEVQLSCKTKDVMCFDVASQYKAWGFRVFEV"
     gene            773..3304
                     /locus_tag="PLT31_04005"
     CDS             773..3304
                     /locus_tag="PLT31_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HPW94333.1"
                     /translation="MKTIVHLFKNLAHPVGLIGTIIAVLIPFLIYLLPNIDQKEFIKN
                     ADLHFPIWLFIIQFIAVISLFIYLHKDLTNWIREHFPSKHLFLAVIGFIAFATIFAGT
                     QIEARHRVQSDESIFLSVAQNLYYNQSSGTCNQGEFKDGGLECYVVSNSFKTKGQPFL
                     YYLGMYLFGTDLQWIFSAQLLMFAATLLLFFLAIFVWTKQPILSFLSVILIASQPTIL
                     FQFRSMSVEPLYFFLSALSILVFKWAYEKNTLTHWILLGLILAFFAQTRQETVFCLFA
                     FILFSLPKILDRRDEKAPAFFLILSIFTLPALLTISYFQGYSFQGGEFKAHGYFLEHL
                     AKNWTVMTQPLKNGELVNPFLTYFNYLFVFGVLALVFSAVREWQQGKQGFSFYSLLFL
                     LLYHIQTYAILENVSGDFSIEINQRYSVVMLPTMAFIAAFGINLLINLLLKFTRKENF
                     LSKPIVTAVILLLSAIGFFGYTMNYKSSFNKNIMYNRNHLTIEEKSILSWLETEPAKK
                     RLFIYGRPWHFIGYGISSVHYNFARSQNEAVLKKWIDDYDGEVYYIRGLDCWDSKTYH
                     QKAVETRIPTTCDVFEREMQLEAVKNILITNNYWVQIAKFKGKKTYQYDQVIQVSPPV
                     IDSTNAVTVSYIQRHTQQEPWETRLYMNQKLLWQEPYAAKNDTRSIEGINTVPGYNTL
                     EFYILDTLKNKNIAYHQSVFFAQNQGATKLIDLAIISHKQDWGELKKNTTIEENPFTV
                     GGKRFTEGFGIHAASTTVFLLDKPYSKLTAFIGLDDESLCSDGVQVVIEGDDQMIYQS
                     QEISPQKVIPIEASIKNVKKLTIKTLSLQSRDCDHVNIIYPTLYE"
     gene            3319..4230
                     /locus_tag="PLT31_04010"
     CDS             3319..4230
                     /locus_tag="PLT31_04010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPW94334.1"
                     /translation="MLLSVIVPLYNEEEIVEKTFLTLEEELKGLNHQIIFVNDGSKDK
                     TREILEKLLESTPHNQLVNFSRNFGHQAAFSAGLQHAKGDAVVIIDGDLQDPPSLIHD
                     MISKWQEGYQVVYAQRNKRRGESLFKRFSAYCFYRVIHALTNIEIPPDTGDFRLMDRV
                     VVDQLNSLPERSRFLRGLVCWVGFKKVGILYDRAERTAGISKYPLRKMIRLAIDGITG
                     FSTTPLKISFLVGLLATIIALGVFIWSILEKVLFPETTVPGWASLMGAIVFFGGIQLI
                     SIGILGEYIGRIYDEVKQRPLYIEDKK"
     gene            4227..4796
                     /gene="gmk"
                     /locus_tag="PLT31_04015"
     CDS             4227..4796
                     /gene="gmk"
                     /locus_tag="PLT31_04015"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HPW94335.1"
                     /translation="MKHRLYVMSAASGAGKTTLKDLVIQEFPDICYSISATTRAPRKG
                     EINGVHYFFKTKKEFEEMIAKNELVEWNLVHENYYGTPKSFVEEQLAQGKRILFDLDV
                     FGKINFDKVYPESTGILILPPSIEELKKRLLQRATDSEEVIQLRLDNAVKEIDYAKKH
                     GKYEYTIINDQLEKAAQELRNILKDPYQA"
     gene            complement(4774..6828)
                     /gene="priA"
                     /locus_tag="PLT31_04020"
     CDS             complement(4774..6828)
                     /gene="priA"
                     /locus_tag="PLT31_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HPW94336.1"
                     /translation="MTKRIPKTPVPENMDKVMTPESFDLFCDVYIPQAPHVYTYGVQR
                     GEVVQKGSVVWVQFNRRKIGLAVVSRVFTERPHFKLKRAVPHLSHYIFSERYMENLEW
                     TAKYYLSSPMTALHAFLPSDFPVLLDAALADILETTCPSNKEAKTIPPCLTREQKEVV
                     DKLTLMLEGDSFRGALLHGVTGSGKTRVYQEVVEKALSLNKKVLILVPEIGLTPQSVA
                     RFSDFLNEPVYVLHSALSKVEKRKTWVEILLGRARVVLGTRSAILAPFDFDLVILDEE
                     HDSSYKQQDPAPRYHCRELAFHLAHKHGALVVLGSATPSVETFSYAKEGNLSYFYLSK
                     RATDIQMPKVHLIDMKKEASLQQTGILLSSPLREALTKCLDEGHQAIILMNRRGYSKT
                     RVCSGCGATLYCKNCHIPLVYHKQHRGLLCHYCGCIHRIDAPCFECGSPTFEFQGGAI
                     EKLEEEIISWIPSAKVVRMDRDTTQNIGSAEEILTSFRDQEYNVLLGTQMVAKGHDFP
                     RVQLVGIVGADIGTGIPDFRMSERLFQLLSQTAGRAGRNQEGGEVWIQTMNPTDPLMR
                     FALTHDYEGFIEMEIQQRREACYPPFCKLLSIEVGARDQTVLQETVQKIETLILPHQS
                     VQLLGPVEGFIPMLKKTFWMRFLVKAESAVAIRNAIKPLTSDLRTLGIPVNTEIKLDM
                     DP"
     gene            6960..9866
                     /locus_tag="PLT31_04025"
     CDS             6960..9866
                     /locus_tag="PLT31_04025"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HPW94337.1"
                     /translation="MSLLNVFRPKWQHSDPEVRIKALQELGPENQDVFETVATSDEAV
                     EVRAMAIRKLTVISALRQLSKEEKDPLIKRTAENKLNEEIVKILKEFRGTASDLELSY
                     VDEIANTRFAEELLKTMPSSELRLALVQKCNKPGFLSVVALKDSKEEVALAAVSAIDS
                     ETLLQDIAKNSRHNTVRQKANEKLKAQKAEPQTATSATMMLFKKREALIQQAKRIIED
                     KSCLSHTDEFNGIIAEASALGMGPAQAELDGLHETFKEKCREELAKKEEEQKQKQLLE
                     LKVGQLDRYLEEFEKILNDNKVKESQLRVNEIIDFWNTHKDNVPAALIKRFTAAISRF
                     DRLSLVKEEKDPSDEKAETIHDDSIVQSMRLEIIEQLKHLAQKDFSEVVSRQVKSLIS
                     NWESLPAILESDVLFETYNSLKNQISETFDKASALQDEIFQSNLVKLQNLIKKIKSID
                     ENVNFRDIAKTLKDSYQEWKEIVGEDKYRYQEIWKEYKEATSRFQEMQQWESWHNERD
                     RETILEEMAFLLKEEPSKEVLFKLRTLSNQWKTIGPVAPSRITELREQFRELFEKIME
                     RCSPLLEEQNAERQKNLEQKEALCERAEKLVSDETIPWKDKFKQFIDLQNEWKTIGQV
                     PKEMNQILWDRFHAITDSFYAEHKERIKQEDLIRQENYEKKQALCEQAEKLQISENWN
                     ETSNKLKKLQEEWKAIGPVPKSQSDEIWNRFRTACDKFFEQKRSHFEEMDEAKQANLK
                     AKEALCEKLESLELDFSNPATLDTLRSIEAEWKAIGMVPKENIESIWERFSSITNKIL
                     ESYAEINEETRNNLNEIKAKKKAMIEQIANLMENAGANQSADITRSLQQEWKTLGSCG
                     IDDFDLHKEFKTICDEFFTRRRDQLDIQEHARKNNLQKKYMLCEQAEQLLENLNESNK
                     EDSMNQVKHLRRLWKEVGAVPREHSDKVWKRFNKACDAIFGKDSSTNTDPVPEA"
     gene            9866..>10080
                     /locus_tag="PLT31_04030"
     CDS             9866..>10080
                     /locus_tag="PLT31_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HPW94338.1"
                     /translation="MSYPPWTSVSEAAALLASGHIVAIPTETVYGLAGNALDPLALAK
                     IFAAKGRPVFDPLIVHIADINSLDNVA"
BASE COUNT         3334 a         1994 c         1799 g         2953 t
ORIGIN      
        1 acttcgaaaa ccctaaagcc ccaagcctta tactgactgg caacgtcaaa gcacatgacg
       61 tcttttgttt tacaagagag ctgcacttca ttggcatcat aaaataaaat caaattggac
      121 aattgaagat gcccagccac acgacctaca ccgtaggcta tttcttcttg aacaccacca
      181 tccgaaatta agaccaccgt tttatgagac acaatatcac caaaacgctc taccataaag
      241 cgttctgcaa tggcagtccc aagagccata ccatgaccaa gacctaatgg tccagaagag
      301 ttttcaatag cgcgatgcac tgcaagctca ggatgcccag gggtttcact attaagttgt
      361 ctaaattcag caagatcaga aagccccata aagcccgtta aagcaagctc agagtataaa
      421 agtggggaca tgtggcctgg atccataaaa aagcgatcgc gacccatcca acttggctga
      481 tctggattaa aacgtaaaaa ttcagcgaat aaaagagtta ttgcatcagc agcccccata
      541 gctccaccgg gatggcccga tttggctttc tggaccattg ccgctgaaag gattcgaaca
      601 ttgtctgctg ctttaagaat taactcattt tgcactgaaa aacctcttta aggtaaaaaa
      661 cattttgcga cgcaaattta atttttcttt actatttcta caagcgataa aacttaaaaa
      721 acctatattc ttactcaatc ttacgttttt tattttgaat tcaaatttta ttatgaaaac
      781 aatcgttcac ttattcaaaa acttagctca tcctgttgga ctcatcggga ccattatagc
      841 cgttttaatc ccctttttga tatacctttt accaaatatt gatcaaaaag agttcataaa
      901 aaatgcggat cttcattttc ccatttggct tttcattatt cagttcattg ctgttattag
      961 tctttttatt tacttacata aagatcttac gaattggatt cgtgaacatt ttccgtcaaa
     1021 acatcttttt ttagcagtca ttggatttat tgcgtttgcc actatttttg ctggaacaca
     1081 aattgaagca agacaccgcg ttcaaagcga tgaaagtatt tttctctcgg tagctcagaa
     1141 tttatactac aatcaatctt ctggcacctg taatcaaggg gaattcaaag atggtggcct
     1201 tgagtgttat gttgtatcca atagttttaa aacgaaaggg caaccttttt tatactactt
     1261 gggtatgtac ctctttggca ccgatctaca atggattttt agtgcccagt tattaatgtt
     1321 tgcagcaaca ttactgctct ttttccttgc tatttttgtc tggacaaagc aaccgattct
     1381 ttctttctta tctgtcatcc tcatcgcctc acagccaacg attctatttc aatttcgatc
     1441 gatgtctgta gaacctcttt atttcttttt atcagcactt tccattttag tctttaaatg
     1501 ggcgtacgaa aaaaatacat taactcattg gattctactc ggtcttatcc ttgctttttt
     1561 tgcacaaaca cgtcaagaaa ccgttttttg cttattcgct tttattcttt tttcactccc
     1621 gaaaatactc gatcgtcgtg acgaaaaagc tcccgctttt tttctcatcc tttccatctt
     1681 tactttacca gcgcttttaa ccatcagcta ttttcaaggt tatagttttc agggcggtga
     1741 gtttaaggct catggctatt ttctagaaca ccttgctaaa aattggactg ttatgacgca
     1801 gcctcttaaa aacggagaac tagtcaaccc atttttaact tactttaact atctctttgt
     1861 ttttggtgtt cttgctcttg tattttccgc cgttcgcgaa tggcaacaag gtaagcaagg
     1921 cttttcattt tattctctct tatttttgtt actttaccat atccaaacct atgctatttt
     1981 agaaaatgtc tctggtgatt tttccattga aatcaatcaa agatatagtg tggtgatgct
     2041 cccgaccatg gcttttatag cagcctttgg catcaatcta ctcatcaatc ttcttctaaa
     2101 atttactaga aaagaaaatt tcctttctaa gccaattgtc acagcagtga ttcttctttt
     2161 aagtgccatt ggattctttg gctacaccat gaactataaa agcagtttca ataaaaacat
     2221 tatgtacaat agaaaccact taactataga agaaaaaagc attttaagct ggcttgaaac
     2281 agaacccgct aaaaaacggc ttttcatcta tggtaggccc tggcatttta taggctatgg
     2341 tatttcttct gttcactata attttgctag aagtcaaaac gaagctgtat taaaaaaatg
     2401 gatagacgac tacgatggcg aagtctatta catacgtggt ctagattgtt gggacagtaa
     2461 aacataccat caaaaagcag tagaaacacg tatccccacc acttgcgatg tttttgaaag
     2521 agaaatgcaa ttagaagcag tgaaaaacat tctgatcacc aataattatt gggtacaaat
     2581 cgcaaaattc aaaggcaaaa aaacgtatca atacgatcaa gtcattcaag tttctccgcc
     2641 agtcattgat tctacaaatg cggtgactgt ttcctacatt caacgccata cacagcaaga
     2701 gccatgggaa acaagacttt atatgaatca aaaacttctt tggcaagaac cttatgcagc
     2761 aaaaaatgac actcgctcta tagaaggaat aaataccgtt ccaggttata atacgcttga
     2821 attttatatc ctagatactc ttaagaataa aaacatagct taccaccaat ctgtcttttt
     2881 tgcacaaaac caaggagcca caaaactcat tgatcttgca ataatttctc acaaacaaga
     2941 ttggggggaa ttgaaaaaga acaccacaat agaagaaaac ccctttactg ttggagggaa
     3001 acgatttaca gaaggttttg gcattcacgc tgcctccacg actgttttct tactagataa
     3061 gccctacagc aaattaacag catttattgg gctagacgat gaaagccttt gtagcgatgg
     3121 agtccaagta gtcatcgaag gcgatgacca aatgatttat caaagtcaag agataagccc
     3181 tcaaaaagta attcctattg aggcttctat aaaaaatgta aaaaagctta ccataaaaac
     3241 actgtcactt caatctcgcg attgtgatca tgtgaacatt atctacccta cactttacga
     3301 ataataattg gagaacttat gcttttatca gtcattgtcc ccttatataa cgaagaagaa
     3361 attgttgaaa aaacattttt aactttagaa gaggaactca aaggccttaa tcatcaaatt
     3421 atttttgtaa atgatggatc aaaagacaaa acgagagaaa tacttgaaaa gcttttagaa
     3481 agcaccccac ataatcaact agttaatttt agccgtaatt ttgggcatca agccgctttt
     3541 agtgcgggct tacaacacgc caagggagat gcagttgtta ttattgatgg cgaccttcaa
     3601 gatcctccaa gcttaattca cgatatgatt agcaaatggc aagaaggcta ccaagtggtt
     3661 tatgcccaac gaaacaaacg ccgtggcgaa tctttgttca aacgtttttc tgcatactgt
     3721 ttttatcgag taatccatgc cctcaccaat attgaaatac ctccagatac aggtgatttc
     3781 cggttgatgg accgcgtggt agtcgatcaa ctgaacagcc tccccgaacg cagtcgtttt
     3841 ttaagaggct tagtttgttg ggttggattt aagaaagtag gaattctcta cgatcgtgct
     3901 gaaagaactg cagggatttc aaaataccct cttcgtaaaa tgattaggct tgcgattgat
     3961 ggaatcactg gttttagtac aacgccctta aaaattagtt tcttggttgg ccttctcgcg
     4021 acaatcatcg ctttaggtgt ttttatttgg agcattcttg aaaaagtact attccccgaa
     4081 acgacagtgc ctggatgggc ttctttaatg ggagcgattg tcttttttgg aggaattcaa
     4141 ctcatttcaa ttggaattct tggcgaatac attggccgca tctacgatga agtcaaacaa
     4201 cgtcctcttt acattgagga taaaaaatga aacacagatt atatgtcatg agtgccgcta
     4261 gtggagcggg caaaacaacc ttaaaagact tagtcattca agagtttcct gacatttgtt
     4321 attcgatttc agcaactaca agagctccta gaaaaggcga aatcaatggt gttcattatt
     4381 ttttcaaaac aaaaaaagag tttgaagaaa tgattgcaaa aaatgaacta gtggaatgga
     4441 atcttgttca tgaaaattat tacggcacgc caaaatcttt tgtagaagaa caactcgctc
     4501 aagggaaacg tattttattt gatttagacg tctttggcaa aatcaacttt gataaagttt
     4561 atcctgaatc tacaggcatc ctgattttac ctccttctat tgaagagtta aaaaaacgtt
     4621 tacttcaaag agcgactgac tcagaggaag tgattcaact gagattagat aatgcggtaa
     4681 aagagataga ttatgccaaa aagcatggta agtacgagta caccattatt aatgatcaat
     4741 tagaaaaagc cgctcaagaa ttaaggaata tcctcaagga tccatatcaa gcttaatctc
     4801 tgtattcaca gggattccaa gcgttcttaa atcagatgtt aatggcttta tcgcatttct
     4861 aatcgctacc gctgattccg cttttacgag aaagcgcatc caaaatgttt ttttcagcat
     4921 cggaataaaa ccctctacag gccctaaaag ctgaacactt tgatgaggta aaatgagggt
     4981 ttctattttt tgcaccgttt cttgcaaaac agtttgatcc cgtgcaccga cttctattga
     5041 aagcaatttg caaaaaggag gataacacgc ttctcgacgt tgttgtattt ccatttctat
     5101 aaagccttca taatcatgag tcaaagcgaa acgcatcaaa ggatctgttg gattcattgt
     5161 ttggatccag acttcaccac cttcttgatt tcgcccagca cgacctgccg tttggcttaa
     5221 caactgaaac aatcgctcac tcattctaaa atcggggatg cctgttccta tatcggctcc
     5281 aacgattccc acgagttgca ccctcgggaa atcatgccct tttgccacca tttgcgttcc
     5341 taaaaggaca ttgtattctt gatctctaaa agaagttaaa atctcttctg cagaccctat
     5401 attttgagtc gtatctcggt ccattcgaac cacttttgca cttggaatcc acgatataat
     5461 ttcttcttct aatttttcaa tagctccacc ttgaaattca aaagtaggag aaccgcattc
     5521 aaaacaaggt gcatctatgc gatgtataca accacaataa tgacaaagta aaccacgatg
     5581 ctgcttatga taaacaagag gaatatgaca atttttgcaa tacaatgttg cgccacaacc
     5641 gctacaaact cttgttttag aataccctct acgattcatc aagataatcg cctgatgccc
     5701 ttcatcgagg cacttcgtta aagcttctct aagaggagaa gacaatagaa ttcccgtttg
     5761 ttgtaaagaa gcttcttttt tcatatcaat caaatgcact tttggcattt gaatatcagt
     5821 agctcttttc gataaataga aataggagag atttccttct ttagcatacg aaaatgtttc
     5881 tacagaaggc gtagcactac ctaaaaccac taaggcccca tgtttatgag ctaaatgaaa
     5941 agcaagttct cgacaatgat agcgaggagc aggatcttgt tgtttgtaag aagaatcatg
     6001 ttcttcatct aaaataacca gatcaaaatc aaaaggagct aaaattgcac ttctcgtacc
     6061 aagcaccact ctcgctcggc caagaagaat ttccacccac gtttttctct tttcaacttt
     6121 tgataatgca gaatgtaaaa catagacagg ctcatttaaa aaatcagaaa agcgagcgac
     6181 actttgggga gttaaaccta tttctggtac aagaataaga acttttttat tcaaggataa
     6241 cgctttttcc actacttctt gataaactct cgtttttcct gaacccgtaa cgccatgtaa
     6301 taaggcacct cgaaaagaat cgccttcaag cattaaagtt aatttatcta caacttcttt
     6361 ttgttccctt gttaaacatg gaggaatagt ctttgcttct ttgttagaag gacaagtggt
     6421 ttcgagaatg tctgccagag ccgcatctaa taaaacagga aaatcggaag gtaaaaaagc
     6481 atgtagagct gtcattggag aagacaaata atacttagca gtccattcta aattttccat
     6541 gtaacgttca gaaaaaatat aatgactcaa gtgtggcact gctcttttca atttaaaatg
     6601 agggcgttcc gtaaacactc tcgaaacaac ggcaagtcca atttttcgtc gattaaattg
     6661 cacccaaaca acactgcctt tttgtacaac ctcccctctt tgaaccccat aagtatacac
     6721 atgaggagcc tgtggaatat aaacatcaca aaacagatca aacgactcag gggtcatgac
     6781 cttgtccata ttttcgggaa caggggtttt aggtattcgt ttagtcatgg aatagctcaa
     6841 aaatagcttc taaaagctat aaaaagcgtt ttttttttat tacttcactt attgatttat
     6901 ttagattatg ttgagtttca tttcctaaat cggaaatgcc atcttatatg gagatcctta
     6961 tgagcttatt aaatgtattt agaccaaaat ggcagcactc tgaccctgaa gtacgtatta
     7021 aagctttaca agaacttggc ccagaaaacc aagatgtatt tgaaaccgtc gcaacaagcg
     7081 acgaggctgt agaagttcgc gccatggcca tacgcaaatt aacagtcatt tctgctttac
     7141 gtcaattatc taaagaggaa aaggatccat taattaaacg cactgctgaa aataagctca
     7201 atgaagaaat tgtaaaaatc ttaaaagagt ttcgaggaac cgcttctgat cttgaactaa
     7261 gctacgtgga tgaaattgca aacactcgtt ttgcagaaga attattgaaa acgatgccct
     7321 cttctgagtt acgtcttgct ttagttcaaa aatgcaataa gccaggtttt ttatctgttg
     7381 ttgctcttaa ggattcaaaa gaagaagtgg ctcttgctgc tgttagtgcg atcgatagcg
     7441 aaaccctatt acaagacatt gcgaaaaatt ctcgccacaa tactgtacgt caaaaggcaa
     7501 atgaaaaatt aaaggcacaa aaagcagaac cacagactgc gacttctgct accatgatgc
     7561 tctttaagaa aagagaagct ttaattcaac aagcaaaacg aattatcgaa gacaaatcct
     7621 gtctcagtca tacagatgaa tttaatggca ttattgcaga agcatctgct cttggcatgg
     7681 gtcctgctca agcggaatta gacggactgc atgaaacatt caaagaaaag tgcagagaag
     7741 aacttgccaa aaaagaagaa gagcaaaaac aaaaacaatt actcgaactc aaagtgggtc
     7801 aattagatcg atatctcgaa gaatttgaaa aaatcttgaa cgataataaa gtgaaagaaa
     7861 gtcaacttcg cgttaatgag attattgatt tctggaatac tcataaagac aatgtgcctg
     7921 cagctttaat caaacgtttt actgctgcaa tttctcgctt tgatcgtctc tcgcttgtga
     7981 aagaagagaa agacccttct gatgaaaaag ctgaaaccat tcatgacgat tctatagttc
     8041 aatccatgag acttgaaatc attgagcaac taaaacacct agctcaaaaa gatttttcag
     8101 aagtagtaag tcgtcaagtg aaaagtttaa ttagcaattg ggaatctcta ccagccattt
     8161 tagagtccga tgtactcttt gaaacctata attctttgaa aaatcaaatc tctgaaacat
     8221 ttgataaggc aagtgctttg caagatgaaa ttttccaatc aaatcttgtg aaacttcaaa
     8281 acctgataaa aaagatcaag agtattgatg aaaacgttaa tttccgtgat atagctaaga
     8341 ctcttaaaga ttcttaccaa gaatggaaag agattgttgg cgaagataaa tatcgctatc
     8401 aagaaatctg gaaagaatat aaagaagcta cttctcgatt ccaagaaatg caacaatggg
     8461 aatcttggca caacgaacga gatcgagaaa cgattctcga agagatggcc ttccttctca
     8521 aagaagagcc aagtaaagaa gtattattta agcttcgtac tttaagcaac caatggaaaa
     8581 caattggccc tgtagctcct tctcgaatca ctgaattacg agaacagttc agagaattat
     8641 tcgaaaaaat aatggaacgt tgttctcctc tcttagaaga acaaaatgcc gaacgccaaa
     8701 agaatttaga acaaaaagaa gccctctgcg agcgcgctga aaagcttgta agtgatgaaa
     8761 ccattccttg gaaagataaa ttcaagcaat ttatagatct acaaaacgaa tggaaaacca
     8821 ttggtcaagt tcctaaagaa atgaatcaaa tcctttggga tcgtttccat gccatcacag
     8881 actcttttta tgctgagcac aaagaaagaa tcaagcaaga agatcttata agacaagaaa
     8941 attatgagaa aaaacaagct ctttgtgaac aagccgaaaa acttcaaatt tctgaaaatt
     9001 ggaatgaaac atctaataaa ttaaagaagt tacaagaaga atggaaagcc attggtccag
     9061 tgcctaagag tcagtccgat gaaatatgga atcgtttccg tactgcttgc gacaagttct
     9121 ttgaacaaaa aagatctcac tttgaagaaa tggatgaagc taagcaagcg aatttgaaag
     9181 caaaagaagc tctttgcgaa aaacttgaat ccttagaact agacttttca aatccagcaa
     9241 cattggatac cttacgctct attgaagcag aatggaaagc cattggaatg gttcctaaag
     9301 aaaatattga atctatttgg gaacgtttct cttctatcac gaataagatt cttgaaagtt
     9361 atgctgagat taatgaagaa acaagaaata atctgaatga aattaaagct aaaaagaaag
     9421 cgatgataga acaaatcgca aacttaatgg aaaatgctgg cgccaatcaa agcgccgata
     9481 tcacaagatc acttcaacaa gaatggaaaa cattaggttc ttgcggcatt gacgatttcg
     9541 atcttcataa ggaattcaaa accatttgtg atgaattttt cactcgtcgt cgtgatcaac
     9601 ttgatattca ggaacatgct agaaagaata atttgcaaaa gaagtacatg ctttgcgaac
     9661 aagctgaaca gcttcttgaa aacctaaatg aatcgaataa agaagattcc atgaatcaag
     9721 taaagcatct ccgtcgactc tggaaagaag tgggtgcagt gccaagagaa cattccgata
     9781 aagtttggaa gcgttttaat aaagcatgtg atgctatttt tggaaaagat tcttctacga
     9841 ataccgatcc tgttccggaa gcttaatgtc ctacccgcct tggactagcg tttccgaagc
     9901 ggccgctttg ctcgcttcgg ggcatattgt agcaataccc accgagaccg tatacggtct
     9961 cgcgggaaac gctttggatc ctcttgcatt agctaaaatt tttgcagcca aagggcgtcc
    10021 tgtttttgat ccccttattg tgcatattgc agatattaat tccctagata atgtggcaca
//
LOCUS       DAOOBB010000056        10025 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_8405_length_10025_cov_4.415647, whole genome shotgun sequence.
ACCESSION   DAOOBB010000056 DAOOBB010000000
VERSION     DAOOBB010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 10025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10025
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8405_length_10025_cov_4.415647"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PLT31_04035"
     CDS             complement(<1..371)
                     /locus_tag="PLT31_04035"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPW94339.1"
                     /translation="MRWDWGSFLAVLSLLILVSLGFASNPIVNTMFTADPAVLVYNDS
                     LIDKEENSIEWLAVSDHLKRQGIGRILVEYAINELDSKRDMKVQTFSKGVEAGTPARN
                     LYQAFGFEDHKNMGKNPAGIET"
     gene            complement(371..2335)
                     /locus_tag="PLT31_04040"
     CDS             complement(371..2335)
                     /locus_tag="PLT31_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HPW94340.1"
                     /translation="MGKGFSFLASVLFLIQGVSAQEWYARENSVGAHDPTILRHEDGY
                     VLQTTNNMLLTSTSEDALNWKTHGRALSAIPSWLKTVTNNGVEDIWAPDLFFFGGEYR
                     SYYTGSAFGQNTSGIGFVSTKSIVPGSSDYNWVDEGEVVRSVSSNNYNAIDADVVEDA
                     EGRYWMAFGSWWTGIKLVRLDESTGKQSSSDKTVYSIANRGGSGIEGPSIIEYKGKYF
                     LFTAWDVCCKMGADLEDNTYKTVMGRADNITGPYVDRSGKSLANGGGTILMQRYGRYY
                     GPGGGEAFKDLNRIRFAHHYYNANSNGSPTLHIRDLVFTEDNWAEMGQPFLGRYLSAE
                     AEHGALTNVEITTGTASNGEYVAYINYADSKVRLPMNIPQAGDYLLRYRYANGDANAS
                     THQVTINGQKQTVNLPSTGAWGTFPENSVVYIPATLKRGGNFIEVGVGTNFGELDRID
                     FLRVIRDTLPANGFDNGIRVRLTGNDEFAIKAGGWALFENVITDSIKGNEVSIQVKNS
                     SGGTLTLHAGSRTSAAISTCTLPSSTSEWTKVECSSLSDLSGVKDFYIKAEGLSQEVL
                     VGNINFEEDGPPISLMKQKTPGLNYQLTKTGPNYTVVFDEPGAYTIDVFNSLGQVLKR
                     VSTSNEKSIELSNLPETKTIIRVQKLGRVK"
     gene            complement(2630..2857)
                     /locus_tag="PLT31_04045"
     CDS             complement(2630..2857)
                     /locus_tag="PLT31_04045"
                     /inference="COORDINATES: protein motif:HMM:NF024050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HPW94341.1"
                     /translation="MADAKLDYKTTILERICYTELVYGRINKKLNLNLSKAEIEKMIF
                     EMIQKTDDSFFQKIGKNIYITNEKKKRCFDH"
     gene            3394..5568
                     /locus_tag="PLT31_04050"
     CDS             3394..5568
                     /locus_tag="PLT31_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HPW94342.1"
                     /translation="MIKRFQILLLTATIAVGSAMAAPQYPFPQNRANPYGNTVKYADP
                     QMIQDHFTKWKGAWYQDKGSEAWVLAPEGTCSTVSEAIAYGMMIMVYMSSSQSDHQSE
                     FDKLWATWKNNSMGGNGGMNWRIGCDGGSGSATDADVDAALALIMASKQWNDSKYLDA
                     AKSLITWMASNDIDGNNSVKPGSNWNDAFNPSYGTLANFELFAKVSSDPKWNSVKTKA
                     GQDLLACQNSKTGLVPDWCSWSDHKPVNTSASVAQNDNPGFFDDAARTPWRMAWAYYW
                     YNNNDAKTFNGKILPWMYAESKGTASNINSGYYPDGTLSSKRDNFVSSTFSGGLGLAA
                     ASDETDAGRNFMGTVYKTLSNMTSCATGNGCGGATPGEKYYPSTLNILYLLLMTGNMP
                     NLYDLNGFTQFTPDPTLAPSISDIDGEQMKKGDTTVGVSGFWNWGAYHDKYNIGTKMV
                     PDSGASPLFRKSGVIYAEASMEIGPEPEWTAEAAAAGTLKYPSAGIAMSFLKSEKGVN
                     LTALGVSKIRINAKVQGPIRVALLNEATVQLGGEPGMYMEPGSDYQDYLINLTPSGYG
                     TTDLEILSWVDESTAPTGENILKVATGLKFELKDSKGGVGSISIKSIEFLKADGSVVD
                     PATITGIIIPEVAIPETPNPDIQGIADPQVQNFAKFQTSVNGTQLQIFNAVSGSDFAV
                     FNMQGKVITKGKIESNTQNVTIPNKGVFMLRVGSVNRLISVK"
     gene            5722..6114
                     /locus_tag="PLT31_04055"
     CDS             5722..6114
                     /locus_tag="PLT31_04055"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HPW94343.1"
                     /translation="MKTIEVVAGIIQKDNKIFATQRGYGDFAGYWEFPGGKMETGESQ
                     EQALIRELKEELNVIVSVDSFFCTVNYDYPSFHLILHCFLCSIISGELTLVEHKAAKW
                     LDKANLLSVNWLPADITVIDSLLQREHN"
     gene            complement(6111..7502)
                     /locus_tag="PLT31_04060"
     CDS             complement(6111..7502)
                     /locus_tag="PLT31_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94344.1"
                     /translation="MNNIQRLFCAIALLSSLVCAWNQRGLADCGGLFLLDQTDLSFSV
                     FRCNATDSVIKNNIMVYGGYGFVQGNAAWNNDWLLEWKKKDHYGQGGFRGDSRYLSLA
                     ADFEFGQARESYSVTSGLKLPDSSFYASGSISRENNQIFNLTWTPEDSFQYIDTISGN
                     WESNLLKKEFNLGMIYHQHHLYSSVSLMDSKRASLEDNHYSIRDSSMIYTGFLKYEYQ
                     LEKGRFSIQGYHLDLESYIYGLRKVEENTKRFLYIPLEIQYNEYWASFLYTHWDFRIG
                     YGNLAIELPQDTRRFYETLSPNRALDNSITQVVSFSFYQRNYRITGNMDAYWLDARAS
                     YSSEKQIKRWHWKRILGFDFFRVQGDMSVIKTSESSNIFGGTSYNEDIMGRFTFVGTH
                     LKIGSRLESPERKIYFDITLGQIIPFYFHHQIKEIKTEKLVQKYDSKEGHETQYKPFR
                     DGFDMRVQLGFIF"
     gene            complement(7517..9256)
                     /locus_tag="PLT31_04065"
     CDS             complement(7517..9256)
                     /locus_tag="PLT31_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94345.1"
                     /translation="MIVATSVIFYSGCSSEVDPASTGEVPKEVDYKVSACADISDSEV
                     QAELDEATNSSIGIFQALASGDFDSAKVVSAKTKSTFKTILTDNPKNCGAQLGYAVTI
                     ISDLVNNKDVNDLYNRVTNEEPSTLLNVDPESYSKILMKTSVLAKSSEQPLITDQVQS
                     AIATGVLPAIDSAIVLLRNIANTEGFSFTFTVEGRTVELDKGEIAPALGVLYFAKAYL
                     IAAASINIDASKDKSYQWALTLSDMNSSDFENLSTEQRAALDHVTGLLSSNSPFTTVR
                     DGWESSWKSIPALLDTAISNVQEGLEYGIEEYNDPKNSQENDLYVVGLDEEADVSPQD
                     LQEAIDSMESIKGYLRGPVEIDITDSLSITVNISKFFTITDGFQDFLPYYKLTDYSTW
                     MTPPPEVMVWEEYLNWELDGSAAAYEIEEAVTSLYQGEVDDLWEGSDAYYVELITPDY
                     EYNSIPVYIDGCSYTIGDGDVAHQLSTTSCKDSNGTIRYQNFNITMPNILIFTDADGK
                     ETLPAYKLTLPKNEDGDNWEISDMKGIIIFPDPTFNGVFPKMKTQDDIWNLIEKIYYL
                     ETSYEDEEDYLYY"
     gene            complement(9567..>10025)
                     /locus_tag="PLT31_04070"
     CDS             complement(9567..>10025)
                     /locus_tag="PLT31_04070"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPW94346.1"
                     /translation="KDSVFFYESSDFLAKGYLIMDSLILVTGFDGIGIDYFTVFSNDS
                     FYKHSPLIEAIDEVVFSRINKVVGIKELKNKLSQPITVNLSRNQLYVVSENKQIERVS
                     IYSMQGEIMKTKIIGHNSFGLNVSDLVHGMYVLEIQLNDQKKIRHSFVIK"
BASE COUNT         3124 a         2026 c         1670 g         3205 t
ORIGIN      
        1 gtttctatcc ctgctggatt tttacccatg tttttatgat cctcaaagcc aaacgcttga
       61 tacaaatttc gagcaggtgt tcctgcctcc acacccttag aaaacgtctg cactttcata
      121 tctcttttag aatctaattc gttgattgcg tactcgacaa gtattcgccc tattccttgt
      181 ctttttaaat gatctgatac cgctagccat tctatgctat tttcctcttt gtcaattaga
      241 ctgtcattat aaactaaaac agctggatct gcagtaaaca tggtattcac aatcggatta
      301 cttgcaaaac ccaaagacac caaaattaat agcgagagca cagccaaaaa tgaaccccaa
      361 tcccacctca ttattttact ctcccaagct tttgtactcg aatgattgtc tttgtctctg
      421 gtaaattgga aagttctatt gatttttcgt ttgaagtaga gacacgcttt aatacttggc
      481 ctagagagtt aaaaacatct attgtgtatg cgcctggttc atcaaaaaca acagtatagt
      541 ttggacctgt ttttgtgagt tgataattta atccaggtgt tttctgtttc attaaagaaa
      601 tcggaggacc atcctcttca aaattaatat tacccacaag cacttcttga gataagcctt
      661 ctgcttttat gtaaaagtct ttaaccccag ataaatccga gagtgaagag cattccactt
      721 ttgtccattc gcttgtactt gatggtaatg tacaagtgga gatcgctgct gaagtacgag
      781 acccagcatg taaagtaaga gtaccaccac tactattttt tacttgaata gaaacttcat
      841 ttcctttaat ggaatctgta atgacgtttt caaataaagc ccacccacct gctttgattg
      901 cgaattcatc gttacccgtt aaacgaactc gaataccatt atcaaagcca ttcgctggca
      961 aagtatcacg aataacacgt aaaaaatcaa tgcgatctag ctcgccaaaa tttgttccca
     1021 cacccacttc gatgaaattt cctccacgct taagcgttgc aggaatatat accacagagt
     1081 tttctgggaa cgttccccaa gcacccgtag aaggcaaatt caccgtttgc ttttggccat
     1141 taattgtcac ttgatgagtg gaagcattag cgtctccatt ggcataacga tagcgaagta
     1201 aataatctcc cgcttgagga atattcattg gcaaacgcac tttactatcc gcataattaa
     1261 tgtaggcaac atactcccca ttagaagcgg ttcccgtagt gatttcgaca tttgtcaatg
     1321 caccatgttc tgcttctgca gaaaggtaac gacctaaaaa tggttggccc atttcagccc
     1381 aattatcttc tgtaaagacc aaatctcgaa tatgtaaagt aggagaccca ttgctattgg
     1441 cattgtaata atgatgagca aaacgaatac gatttagatc tttaaacgct tcaccaccac
     1501 cagggccata ataacggcca tagcgttgca ttaaaatcgt accgcctcca tttgcaagtg
     1561 attttccact acgatctaca tatggcccag tgatattgtc tgctcgtccc attaccgttt
     1621 tataagtatt gtcttcaaga tctgctccca ttttacagca aacatcccac gctgtgaata
     1681 agaaatactt ccctttatat tcaataatac ttgggccctc tataccagag ccacctcgat
     1741 ttgcaatact ataaaccgtt ttatcagaac tagactgttt gcctgtagat tcatctaagc
     1801 gaaccaactt gattcctgtc caccatgatc caaaagccat ccaatagcga ccctcggcat
     1861 cttcaactac atcagcatca attgcattat aattgttact agaaacactt cgaaccactt
     1921 ctccttcatc tacccaattg tagtcagaag atccaggtac aattgattta gtggagacaa
     1981 aacctatacc cgatgtattt tgtccaaaag cggaaccagt gtaataactg cgatattcgc
     2041 ctccaaaaaa gaagaggtct ggagcccaaa tatcctccac accattatta gtcactgttt
     2101 ttaaccagct agggattgca gataaagctc tcccgtgagt tttccaattc aaagcatctt
     2161 cagaagtact cgtcaataac atattgttag tcgtttgcaa aacatagcca tcttcatggc
     2221 gaagaatcgt agggtcatgt gcccctacac tattttctct tgcataccat tcttgagcgc
     2281 ttaccccttg aattagaaat aaaacactcg ccaaaaagga aaaaccttta cccatataca
     2341 ccacccttat tgtcatccaa atcttactcg aaatataaac aataatggtt aatagtgcaa
     2401 ataaaagtct acgttttaac gcaaataaat gttaattcaa atgtttttta acattacaag
     2461 atgaatttaa gtctacactt agtaattatt ttgacactat tttttccatc ctcaaatgct
     2521 ctttttttct ataaaacccg ttttttgtaa aaaaggggaa agctaacgac aatgagatca
     2581 gcactatttt ttgattctat ctgctgtaat aattcggtag gtgtaagaat taatggtcaa
     2641 aacagcgttt ttttttctca tttgtaatgt aaatgttctt gcctattttt tgaaagaaag
     2701 aatcatccgt tttttgaatc atctcaaaaa tcattttttc aatttcggct tttgataaat
     2761 tcaggtttaa ttttttatta attcgaccat aaacaagctc tgtgtaacat attctttcta
     2821 aaatggtcgt tttataatca agttttgcat ctgccatgag tcaccttgtt tttttgaata
     2881 actttgtggg ttctggtctt tgaaaaaccc ataatttaat gtttcttatg cgaagagcaa
     2941 tatagttttt atcaattatt cagagtgatt tttgcaaata catttcactt cgtttttaag
     3001 gatggtttgt acatataaca tttacttaac cttcattttc gtgcccgtag tgcttagcgt
     3061 gaaaattgcc gagcgtagcg actgcaaaaa gtacagcaaa aggaattatt aggttcaaga
     3121 atagtcagta tttacctgct tcgtctcggg cacgattaaa tgtaaacaac atttctttac
     3181 tgtaaatatt ctattacaat gacacttatt tttttctaaa agcccgtttt tgtcacaaaa
     3241 acaattgttt ttaattacaa tgcaacatat ttattacatt caaaaacgaa acgattcgta
     3301 gataagattt tatattctct gtaactagtt gtaagctagt tccttttttt tgggatgcgt
     3361 gttgggaacg tccattatca caaagggaac ataatgataa aaagatttca aatccttctt
     3421 ttaactgcaa cgatagcagt cggttcagcg atggccgctc cacagtatcc atttcctcaa
     3481 aacagggcaa acccatacgg caataccgtt aaatatgccg accctcagat gatccaagat
     3541 cattttacta aatggaaagg agcatggtat caggacaagg gttctgaagc ttgggttctt
     3601 gcccctgaag ggacctgctc cactgtttct gaggcaatcg cttatggtat gatgatcatg
     3661 gtttatatgt ctagctcgca aagcgatcat caatcggagt tcgacaagct ttgggctact
     3721 tggaaaaaca actctatggg tggaaacggc ggtatgaact ggcgtatcgg atgcgatggt
     3781 ggttctggat ctgctactga tgccgatgta gacgctgctc ttgctctcat catggcctcc
     3841 aagcaatgga atgacagcaa gtatcttgac gctgcaaagt cccttattac ttggatggct
     3901 tctaacgaca ttgatggcaa caattctgta aagccaggta gcaattggaa tgatgctttc
     3961 aacccaagct atggcactct cgctaacttt gagcttttcg ccaaagtttc tagtgacccc
     4021 aaatggaatt ccgtaaagac caaagctggc caagatcttc ttgcttgtca aaattctaag
     4081 actggtcttg ttcctgactg gtgttcttgg agtgatcata aacctgtgaa tactagtgct
     4141 tccgttgctc aaaacgataa tcctggtttc tttgacgatg cggctcgtac tccatggcgt
     4201 atggcttggg catattattg gtacaataac aatgacgcca aaacatttaa tggtaaaatc
     4261 ctcccttgga tgtacgccga aagtaaaggt acagcaagta atattaattc tggttactat
     4321 cctgatggga ctctttcttc caagcgcgat aactttgttt cttctacctt ttctggcggt
     4381 cttggtcttg cagcagcttc tgatgaaacc gacgctggca ggaatttcat gggcacagtc
     4441 tataaaaccc tttctaatat gacaagttgt gctacaggta atggctgtgg cggtgccact
     4501 cctggagaaa aatattatcc atctacttta aatattcttt atttgcttct aatgacaggc
     4561 aatatgccta acctctacga cttgaatggt ttcacacagt tcactccaga tccaacactt
     4621 gctccttcta tttcagatat tgacggcgaa caaatgaaaa aaggcgatac gacagttggc
     4681 gtttctggat tctggaactg gggagcttat catgataagt acaacatcgg aaccaagatg
     4741 gtaccagatt ctggtgcatc tcctcttttt agaaaaagtg gtgtcattta cgctgaagca
     4801 agcatggaaa tcggccctga acctgaatgg actgctgaag ctgctgctgc gggtacccta
     4861 aaatacccaa gtgcgggtat tgcaatgtcc ttcctcaaaa gcgaaaaagg ggtgaattta
     4921 accgctcttg gtgtttcgaa aatacgtatt aatgctaaag ttcaaggccc tatccgcgtg
     4981 gctcttctaa atgaagctac tgtacaactt ggtggtgaac ccggtatgta tatggaacca
     5041 ggttctgact accaggatta tttaatcaat cttacacctt ctggctatgg aactacagat
     5101 ctagaaattc ttagttgggt tgatgaaagt actgccccta ctggtgaaaa tattcttaaa
     5161 gtcgcaacag ggcttaagtt tgaattgaaa gactcaaaag gtggtgtagg ttctatctct
     5221 atcaagtcca ttgaattctt aaaggctgat ggttctgtgg tggatcctgc tacaattact
     5281 ggaattatca ttcctgaagt tgccattcct gaaaccccaa atccagatat acaaggaatt
     5341 gctgatccac aagtgcagaa ttttgccaag ttccaaacaa gcgtcaacgg cactcagctt
     5401 caaattttca atgctgtatc tggttctgac tttgcagtgt ttaatatgca aggtaaggtc
     5461 attacaaaag gtaaaatcga aagcaacaca caaaacgtta ctattcctaa caaaggtgta
     5521 tttatgctcc gcgttggatc tgtaaatcgt ttgattagcg taaaataata ttgcttttct
     5581 tctctctttg aaagtcgcgg ttataccgcg acttttttat gccgtaaaat cagaaagaat
     5641 acgaaatctc caaaaaatgc ataaccctaa atacatcgta caagaaaagc taaattatca
     5701 aaaaatgctt ttaggaaaat catgaaaaca attgaagtcg tagctggaat tattcaaaaa
     5761 gacaataaaa tttttgccac acaacgtgga tatggtgatt ttgcaggtta ctgggaattt
     5821 cctggtggga aaatggaaac aggtgaatcc caagagcaag ccctaataag agagcttaaa
     5881 gaggaactca acgttattgt ttctgttgat tctttttttt gtactgttaa ttatgattac
     5941 ccttctttcc atttaatatt gcattgcttt ctttgctcca ttataagtgg cgaattaacg
     6001 ttagtagaac acaaagcagc taaatggtta gataaagcaa atctactttc tgtaaattgg
     6061 ttacccgctg atattactgt aattgattct cttttacaga gggaacacaa ctaaaaaata
     6121 aaacctagtt gtacgcgcat atcaaaccca tcgcgaaaag gtttgtattg tgtttcatgg
     6181 ccctctttag aatcatattt ttggactaac ttctccgttt ttatttcttt tatttgatga
     6241 tgaaaataaa agggaattat ttgaccaaga gttatatcaa aataaatttt tctctctgga
     6301 ctttccaatc gagaaccaat ctttaaatga gttccaacaa aagtaaaacg ccccattata
     6361 tcttcgttat aagaagttcc accaaaaata ttactggatt cactcgtttt tataacactc
     6421 atatcgcctt gtactctaaa gaaatcaaaa cctaatattc ttttccaatg ccatcgtttt
     6481 atttgttttt ccgatgaata cgatgctcga gcatctagcc aataagcatc catatttcct
     6541 gttatgcgat aatttctttg ataaaagcta aatgaaacca cttgggtaat ggaattatct
     6601 aaagcccgat ttggagaaag tgtttcgtaa aaccgtcttg tatcttgtgg aagttctata
     6661 gctaaattcc cataaccaat acgaaaatcc cagtgagtat ataaaaatga agcccaatat
     6721 tcattatact gaatttctaa gggtatatat aaaaaacgtt ttgtattttc ttctactttt
     6781 cgtaacccat aaatatatga ctctaaatcc aaatgatagc cttgaataga aaatcggcct
     6841 ttttctaatt gatattcata ttttagaaaa cctgtataaa tcatcgatga atcacgtata
     6901 ctataatgat tatcctctaa agaagctcgt tttgaatcca tcaaactaac acttgaataa
     6961 agatgatgtt gatgatagat catcccaaga ttgaattctt tttttaataa attactttcc
     7021 caatttccag aaatcgtatc tatatattgg aaagaatcct ctggagtcca tgtcaagtta
     7081 aagatctgat tattttcacg actaatagat ccgcttgcat agaaggaaga atctggtagt
     7141 tttaatccag aggttacaga atatgattcc cttgcttgcc caaattcaaa gtccgccgct
     7201 agagacaaat aacgagaatc accacgaaag ccgccttgac cgtaatgatc tttctttttc
     7261 cactctagaa gccaatcatt attccaagca gcattgcctt gaacaaagcc gtaaccgcca
     7321 taaaccatga tgttattttt tatgacagaa tcggttgcat tacaacgaaa tacagaaaaa
     7381 gacagatctg tttgatctaa taaaaaaagc ccgccacaat cggcgagccc tctttgattc
     7441 catgcgcaca ctaggctcga aagcaaagcg attgcacaaa ataaacgttg aatattattc
     7501 acaaataagc catttatcaa taatacaaat agtcctcttc atcttcatat gacgtttcca
     7561 aataataaat tttctcaatc aaattccaga tatcatcctg agttttcatt tttgggaaca
     7621 ccccattgaa tgttggatct gggaatataa taatcccttt catatcagaa atttcccaat
     7681 tatccccatc ttcattcttt ggcaaagtca atttgtatgc tggaagagtt tcttttccat
     7741 ctgcatcagt aaagattaaa atattaggca ttgttatgtt gaaattctga tacctgatag
     7801 ttccattaga atctttacaa gacgttgttg aaagttggtg tgcaacatct ccatcaccaa
     7861 tagtataact acaaccatca atataaacag gaatagaatt gtattcataa tctggagtga
     7921 taagttcaac ataataagca tcgcttcctt cccaaagatc atctacctct ccttgataaa
     7981 gacttgttac agcttcctca atttcatatg cagcagcaga accatctaat tcccaattaa
     8041 gatattcttc ccataccatt acttctggag gaggagtcat ccaagtagaa taatccgtca
     8101 atttgtaata agggaggaaa tcttggaaac cgtctgtaat cgtaaagaat tttgaaatat
     8161 tcactgtaat agatagagaa tcggtaatat ctatttcaac agggccgcgc aaatagccct
     8221 ttattgattc catggaatcg attgcttctt gcaaatcttg tgggctaaca tctgcttctt
     8281 catctaatcc aactacatat aaatcatttt cttgtgaatt cttaggatca ttatattctt
     8341 caattccata ttctaagccc tcttgtacat tagaaatcgc agtatctaaa agggcaggaa
     8401 ttgacttcca agaagactcc caaccatcac gtactgtggt aaaaggacta tttgaactta
     8461 agagacccgt cacatgatcc aatgcagctc tttgctcagt ggagagattc tcgaagtctg
     8521 aggaattcat gtcagaaaga gttaaagccc attgataaga tttatcttta gaggcgtcaa
     8581 tgtttatact tgcagcagca ataaggtaag cctttgcaaa ataaaggaca ccaagagcag
     8641 gagcaatttc acccttatct aattcaaccg ttcttccttc tacagtaaaa gtaaaagaga
     8701 aaccctctgt atttgcaata tttcttaaga gaacaattgc agaatcaata gcaggaagaa
     8761 cgcctgtagc aatagcagat tgaacctggt ccgttattag tggttgttca ctactttttg
     8821 ctaaaacact tgttttcatt agaatctttg aataagattc tggatccaca ttcaacaaag
     8881 tacttggttc ttcattcgtt actctgttat aaagatcatt cacgtcttta ttattaacta
     8941 agtcagagat gatggtaact gcataaccga gttgagcacc acagtttttt ggattatcag
     9001 tgagaatagt tttaaatgtg cttttggtct ttgcactaac cactttggca ctgtcaaaat
     9061 ctccacttgc aagagcttga aaaataccaa tgctactgtt tgtcgcttcg tcaagttccg
     9121 cttgaacttc agaatcagag atatctgcac aagcactgac cttgtaatca acttcctttg
     9181 gaacttctcc tgtagaagca ggatccacct ctgaagaaca gcctgaataa aaaatgacgg
     9241 atgtggctac tatcatcccc caaaatttct ttgatccaaa cataagcgtc tcctgaaaaa
     9301 attattattg aatatgtaac ttatttttga cttactttgt taaaaatata gcactttttg
     9361 taatatcctt gccacttttt caaataatag ttctctatca tttttttgtt ttttattaaa
     9421 ataatgcatt tttttcgttt ttacccgtta atttctaaat acattctttt tcccaaaaaa
     9481 aaattagggt gactgtcatc accccatttt gaaatatata atccgtttct aaaacaaaaa
     9541 gagttatttc tcagcgaata caacttttat ttaatcacaa atgaatgtct tattttcttt
     9601 tgatcgttaa gctgaatttc taaaacgtac attccatgaa ctaaatcaga aacatttaag
     9661 ccaaaagaat tgtggcctat tattttcgtc ttcatgattt ctccctgcat ggaatatata
     9721 ctaactcttt cgatctgttt attttcagaa acaacgtata attgattcct cgatagatta
     9781 actgtaatag gttgagataa cttgttcttt aattctttta ttccaaccac tttatttata
     9841 cgtgaaaaaa caacttcatc tatagcctct attaacggag agtgcttata aaaagaatca
     9901 tttgaaaaca ccgtaaaata atctatccct ataccatcaa atcctgtaac tagaattaaa
     9961 gaatccatta ttaaatagcc cttagccaaa aaatctgatg attcataaaa aaagacggag
    10021 tcttt
//
LOCUS       DAOOBB010000057         9927 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_8506_length_9927_cov_4.676661, whole genome shotgun sequence.
ACCESSION   DAOOBB010000057 DAOOBB010000000
VERSION     DAOOBB010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 9927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9927
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8506_length_9927_cov_4.676661"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /gene="efp"
                     /locus_tag="PLT31_04075"
     CDS             complement(<1..565)
                     /gene="efp"
                     /locus_tag="PLT31_04075"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPW94347.1"
                     /translation="MGIVSTNEFRKKLKIMVDGQPYMIIDNQFVKPGKGQAFNRVRIK
                     NLVTGRTLDRTWKSGETVEEAEVNYSEMTYLYNDGATWFFMDKTSMETMEISKEAMNG
                     AEKWLLDGADVEVTWWDGKAIEVIPPTFVDLLIVDSPPAVQGNTSGNVMRPAILETGA
                     EVMIPLFIEQGTKIRVDTRDGSYLERAK"
     gene            606..1556
                     /locus_tag="PLT31_04080"
     CDS             606..1556
                     /locus_tag="PLT31_04080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HPW94348.1"
                     /translation="MNDSSFIFKLLTDLLAYLELPMDEKYDLTPSFLLQVPIHYAQKI
                     EKGNPLDPLLLQILPLLEENKQKPGFTKDPVGDLNATQEPGILQKYNARALVISTCAC
                     SLHCRFCFRKHFNYESTEALPDRLDRWLANHPDIKEIIFSGGDPLFTSPAFFKKLNDS
                     VLKHETVKTIRFHSRLPVTDPDRLEPLLPILKTIPSQKNAILVSHVNHAQELDEKSSA
                     LYKTLREAGFTLLNQSVLLRGINDTVKALVDLSNQLFSQGVLPYYLHQLDKAEGTSHF
                     EVPDHKAIHLLENIRIELPGYLVPRLVREIANEKSKTPLC"
     gene            complement(1565..2935)
                     /locus_tag="PLT31_04085"
     CDS             complement(1565..2935)
                     /locus_tag="PLT31_04085"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPW94349.1"
                     /translation="MFKKVTLISFIFFFSGLSAFASCVIKEVSFDGYWQEEHHRLYRE
                     ILNEPCSALEKANQTVIEWHENHGFPAAKIQSKISEDGKVKVILVRGEAWVWASPENQ
                     GKTKTKKNLWPKITGIEEGKPFSLSEIKKAESQVLRLGYYDKAKETQLYKDGRRNRLI
                     PVFFMKDRPVNHLEGFFSYSSVDHEYSGSIEIALLNMAGTARDLYLEGETGLWGRSLL
                     LKYKEPWLLGSAWSGLIRGMIDEDSLTKEALLEVGVSRRIFEQMELAILGGIGNDEWR
                     TALEVNYLTLDRFILPRSGVSMDASMIIIQKRDSLENALVALTGKGIYLIPIYTKGVL
                     RFSFGAGTLLPSRENHELEDLFSLGGVDSFKGYRNGFFRSRAYGYSECMLEWQAANST
                     AFQAFYQPGIYRARAPEHGWKEINSYGLGISQYRDRWNISIYYAMNLESSFLEGLLHL
                     NVKTLF"
     gene            complement(2935..5799)
                     /locus_tag="PLT31_04090"
     CDS             complement(2935..5799)
                     /locus_tag="PLT31_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94350.1"
                     /translation="MKNHDRLGFIFSIICCFHLASWALNLDSLPVWKGNEIQIDNEAQ
                     TPVQKDSLKLKTQGSKTLQVVVGDGGTQVDQELRLSIQGEVYPSVFVDALLSDVGRSP
                     ADQNTATLQEVDQIYFKVESPYVSLHLGDFSWKQDEFGLLGLERSSLGIGAAIRYSNT
                     EVRGAYGTDEVLREVHVFNGVDGQRSGYIVNTDGLYQTIVPGSEKVWLNGTALKKDVD
                     YDLNYAGGVLDFKGIHLPGAQDEIRIEYDSYASQSISTLKAFEGRYRSKNISLDVGGF
                     RLSSDVERLKRGTWTENDYEKLKADSGNGALWSDSLGSLERPVQTDRMSARIRAQANR
                     RWFLDAEMAYQKKDTNIVSDYVDGPVGRASRWVLSSDSSTNLIHFPIAISLYGNYYET
                     GFLNTLFQGTDRDWESYSLNEEWDLDSSLLTSALRHDHLNIKWRLPLHLFAGVEMGYR
                     QSTDTSLWNASRARVFLEHRHQKVKNSLSLVRVNAVQLLETERYQGIIESRFLEGPFK
                     PYALASYSFWNKDSLAYLFYSDRFKAGTGFEASFYDLKIGEDFAVQQSRRGESKGDLE
                     DSLSLWQWTQKIEVDKKSFQLAHLLHYKNAWTDSIGTADSWLGEISFLTKPNQRGFSL
                     SSSFELGLTNEQTYVPIYKAVAPGTGDVLYDSLTGTFIEGVDNGNYMYEGMGRIDSLG
                     SVRASTAAMDFKAEWNPAKMFAIKNGILRDISFTLQGHFEGRDTTGKTIYAPPFKKQT
                     LEALTSGIVHYEGSLLWQYPSGIASVIYSMGYEDEKKNSVFYYFQNKKWHSGDVRFNI
                     NLLNEISIFAKREEVDLVSLQEMNWVLWDLKASWKRYLFTHFAIEPSAFFRKGDGVDE
                     ISDLNLFLKQGQIKLSWEKSKAATAYALFSATHVSTEGYSLPYQMNSGFSPGLTYRIE
                     ANASFNINDYISFSLRYLIRFGNAEEDLFQKWTTEAKAYF"
     gene            complement(5802..6275)
                     /locus_tag="PLT31_04095"
     CDS             complement(5802..6275)
                     /locus_tag="PLT31_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94351.1"
                     /translation="MEGIPPLRYLRKTTLIIAVVFFSFFVHRIYVSLKSDFVEPVVET
                     VSLLSPEQSVVCRNIVDGAPFGVDSIFEENTRLYFHSHLPIEKEGSSIDSVPFKQIWF
                     IGLDTMLVSNCKEQENICVSSIAPNLLRAGEWSVDLVQGRKLWTSRQFKVESKGL"
     gene            complement(6288..6704)
                     /locus_tag="PLT31_04100"
     CDS             complement(6288..6704)
                     /locus_tag="PLT31_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HPW94352.1"
                     /translation="MSDFAVYSEDAEKVRRLMLAYQGSVKADYVVLCHRDGSIIAEVG
                     ALGVDATPLAVLSTASFDSAKQIGMMLGGENFQSVSYGGENRSIYISPVNESLLLVQI
                     FPGTKLPNRIEDYNRLLVEKLVDAVPAFTQNTSKLI"
     gene            complement(6738..7703)
                     /locus_tag="PLT31_04105"
     CDS             complement(6738..7703)
                     /locus_tag="PLT31_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HPW94353.1"
                     /translation="MSSINFATREISCKVVYYGPGLSGKTTNLQVIHQKMPQDKRTDM
                     VSLATEGDRTLFFDFLPLNLGDIKGFKTRFQLYTVPGQVYYNSTRKLVLRGADGIVFV
                     ADSQRSRQAENLESLQNLRQNLQDYGMDLDDMPFVLQYNKRDMDDVFTLDEMNKYLNP
                     RSIPFFPATAHNGKGVVTTLKMIAMLVIEKFNVKQGFLRKAAESVNNAGVHDLTLNKN
                     GVSMTDIPAKEDSKSGPPDLQSSGAPNSPFKMPSFAAKAPAPEPPRTGNAGLFQKSAL
                     ASPFGRPKVGTPPPQRMPTFGREVPKPNQDVSEDDIELRPYVPKK"
     gene            complement(7916..8185)
                     /gene="rpsT"
                     /locus_tag="PLT31_04110"
     CDS             complement(7916..8185)
                     /gene="rpsT"
                     /locus_tag="PLT31_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HPW94354.1"
                     /translation="MPNHLSCKKRLRQAEKANAVNRSVRSAIRTSLKTIRTATSKEDA
                     VKEMPNLFSMLDKAAAKGRAGFSANRAANYKAKVSKVISNLAAKA"
     gene            complement(8262..8951)
                     /locus_tag="PLT31_04115"
     CDS             complement(8262..8951)
                     /locus_tag="PLT31_04115"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPW94355.1"
                     /translation="MNMGSESLRLGTIYFLAIVLSLAFHEAAHALMAYVLGDSTAKDR
                     GRLTLNPLVHLDTLGTIMIAFMAFQGMGIGWAKPVPVDTNSLKKSRYIGLVSLAGPLS
                     NLILAVLGFQLFLLFQAILLPNIGLHVNIERLIIDFLRVFVLVNIALTAFNLLPVYPL
                     DGAKVVSSLLPPTLSRQFDAFFIKFGSWPLLFFVVWEWFFPFPGPLSFILGPFTRFLL
                     QVLEKSAFWIM"
     gene            complement(8961..9737)
                     /locus_tag="PLT31_04120"
     CDS             complement(8961..9737)
                     /locus_tag="PLT31_04120"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HPW94356.1"
                     /translation="MNTFAEWAPIFEIMRKASQAVLDSYKSDFTVSSKEDATPVTSAD
                     LEANQIIMDGLREYYPEIPIISEEIQNSSIEERSSWNRFFLIDPIDGTSEFVAHQGDF
                     TVNIALIENGLAVLGFVAVPVTGELFVGNTQESWVTPLWNKEDFQSRRLIKESWDLNQ
                     MNPESKILVSVLHFDQPTEEYVSSLGGTVLPIGSSLKFCRIADGTANYYPRLRKLHEW
                     DIAAGHAILKGALGNVYLWKTKTELRYGSSLFETEPFEAY"
BASE COUNT         3179 a         2099 c         1671 g         2978 t
ORIGIN      
        1 attttgcacg ttctaaataa gaaccatcac gcgtatccac gcgaatcttg gtgccttgtt
       61 caataaatag agggatcatc acttcagcac ctgtttctaa aatcgcagga cgcatcacgt
      121 taccactagt attgccttga actgctggag gagaatccac aatcaataaa tccacaaaag
      181 taggaggaat tacttcaata gccttaccat cccaccaggt gacttcaaca tcagcaccat
      241 ctaaaagcca cttttcagcg ccgttcatag cttcttttga aatttccatg gtttccatag
      301 aagtcttgtc cataaagaac catgtagcac catcgttata gagataagtc atttcagaat
      361 aattgacttc ggcttcttct acagtttcac cactcttcca agtgcggtct aaagttctgc
      421 ctgtaactaa attcttaata cgaacacgat taaaagcttg acctttacct ggttttacaa
      481 actggttatc aataatcatg taaggttgac catcaaccat gatttttaac tttttacgaa
      541 attcattagt actaactata cccataaaaa tcctttttta aatttgattg taaatttaac
      601 atcttatgaa cgattcatct tttattttta agcttttaac tgatctttta gcgtatttag
      661 aacttccaat ggacgaaaaa tacgacctta ccccgtcgtt ccttcttcag gttccgattc
      721 attatgctca aaaaattgaa aagggtaatc ctttagaccc cttattatta caaattctcc
      781 cgctactaga agaaaacaaa caaaaacctg gttttaccaa agaccctgtc ggagatttaa
      841 atgctaccca ggagccaggt attcttcaaa agtacaacgc aagagcttta gtgatttcca
      901 catgtgcttg tagtctccat tgtcgtttct gctttcgaaa acattttaat tacgagtcta
      961 cagaggccct tcctgatcga ttagaccgct ggttagccaa tcatccagat atcaaagaaa
     1021 tcattttttc tggaggagat ccacttttta cgtctcctgc cttttttaag aaactcaacg
     1081 atagtgtttt aaagcatgaa accgtaaaaa cgattcgttt tcattctcgg ttaccagtga
     1141 ctgacccaga ccgccttgag cccttactac ccattttaaa aacgattcct tcgcaaaaaa
     1201 acgccattct agtaagccat gtaaaccacg cccaagagct tgatgaaaaa tcctctgctt
     1261 tatataaaac acttagagaa gctggcttta cacttttaaa ccagtctgta ttgctccgtg
     1321 gtattaatga tacagtaaag gctttggtgg atttatcaaa ccaattattt tctcaagggg
     1381 tactccccta ttatttacat caattggata aagcagaagg gacttcccat tttgaggttc
     1441 ctgatcacaa agccattcat ttacttgaaa atattcgaat cgaattgcca ggctatttag
     1501 tccccagatt agttcgagaa atcgctaatg aaaaaagcaa aacgcctttg tgctaattaa
     1561 gctactaaaa caacgttttc acatttaggt gtaaaaggcc ttctaaaaag gatgattcta
     1621 aattcattgc gtaataaata gaaatattcc agcgatctcg atattgggaa atacctaagc
     1681 cataactgtt tatttctttc catccgtgtt caggagctct cgctctgtaa attcctggtt
     1741 ggtaaaaagc ctgaaaagcg gtcgagttag ccgcttgcca ttcgagcatg cattcactat
     1801 agccataagc acggcttcta aaaaagccat ttcgatagcc tttgaaacta tccaccccac
     1861 caagggaaaa taagtcttct aattcatgat tttctctaga aggaagaagt gttcctgctc
     1921 caaaagaaaa gcgaagaact ccttttgtat aaataggaat taaataaatg ccttttcctg
     1981 ttaaagcgac tagtgcgttt tcaagagaat ctcttttctg aataataatc atcgaagcat
     2041 ccattgaaac gccagaacga ggcagtataa agcgatctaa agtcaaatag tttacttcta
     2101 atgccgttcg ccattcatca tttccaatgc cacctaaaat cgctagttcc atttgttcaa
     2161 agatccttct agaaacaccc acttctaaaa gagcttcttt ggttaaggaa tcttcatcaa
     2221 tcatgcctcg aattaagcca ctccatgcag aaccgagaag ccatggttct ttatatttta
     2281 aaaggaggct tctcccccat aatcctgttt ctccttcgag gtaaagatct cttgcggtac
     2341 ctgccatatt taggagcgca atttctatag agcctgagta ttcatgatct acgctggaat
     2401 agctaaaaaa accttccaaa tgattaactg gccgatcttt cataaagaaa acaggaatca
     2461 aacggtttcg tctaccatcc ttgtagagtt gagtctcttt tgctttatca taatacccta
     2521 aacgcaatac ttgagattct gctttcttaa tctcacttaa agaaaaaggc tttccttctt
     2581 ctattcctgt tatttttggc caaaggtttt tcttagtttt tgttttccct tgattctctg
     2641 gagacgccca tacccaagct tcacctcgga ctaaaatcac cttaactttt ccgtcttcag
     2701 aaattttgct ttgaattttt gctgctggga aaccgtgatt ttcatgccac tcgatgaccg
     2761 tttgatttgc tttttctaaa gcagagcaag gttcatttag aatttctcga tatagccgat
     2821 gatgttcttc ttgccaatag ccatcaaaac tcacttcttt aatcacacaa gaagcaaaag
     2881 cagagagccc gctaaaaaag aagatgaaag aaatgagtgt gactttttta aacattagaa
     2941 ataggccttt gcttcagtgg tccatttttg aaataaatct tcttctgcat ttccaaaacg
     3001 aatgagatag cgtagagaga aagaaatata gtcattgata ttaaatgagg cattagcttc
     3061 gatacgataa gtcaaaccag gagaaaagcc tgaattcatt tggtaaggca aagagtatcc
     3121 ttctgtagaa acatgagtcg cactaaataa tgcataagca gtggccgctt tagacttttc
     3181 ccaagataat tttatttggc cctgttttaa aaataaattc aaatcactga tttcatccac
     3241 gccatcgcct tttcggaaaa acgcactagg ttctatggca aagtgtgtaa acaaatagcg
     3301 cttccaagac gctttaagat cccacaagac ccagttcatt tcttgtaaag aaactaaatc
     3361 cacctcttct ctttttgcaa aaatagagat ttcattgagc aaattaatat taaaacgaac
     3421 atctcctgaa tgccactttt tattttgaaa gtaataaaaa acagaatttt tcttttcgtc
     3481 ttcatacccc atagagtaaa tgactgaagc gattcctgaa gggtattgcc atagtaaaga
     3541 gccttcgtag tgcacgatgc cacttgtgag agcttcaaga gtctgttttt taaaaggggg
     3601 tgcataaata gtttttcctg tagtatctcg cccttcaaaa tggccttgta aagtaaaaga
     3661 aatgtctcgt aaaatcccat ttttaattgc aaacatcttt gctggattcc actctgcttt
     3721 aaaatccatc gccgccgtag atgctcttac agagcctaaa gaatcgatgc gtcccattcc
     3781 ttcatacata taattaccat tatctactcc ttcgataaat gtgcctgtga ggctatcata
     3841 aaggacatcg ccagtgccag gagcgacggc tttgtaaatt ggaacataag tttgctcatt
     3901 ggtgagtcct aattcaaatg atgagcttaa agagaatcct ctttgatttg gttttgttag
     3961 aaaagaaatt tctccaagcc aagaatctgc agttccaata gaatctgtcc aagcattttt
     4021 ataatgcaag agatgagcaa gttgaaatga tttcttatcg acttctattt tttgggtcca
     4081 ttgccataaa gatagagagt cttctaaatc tcctttcgat tcacctcgtc tcgattgttg
     4141 tactgcaaaa tcttctccaa tttttagatc ataaaaacta gcttcaaaac ctgttcccgc
     4201 tttaaatcga tcgctgtaaa ataaatatgc taaagagtcc ttattccaaa agctataact
     4261 cgctaaagca taaggcttaa aagggccttc taaaaatcga gattcaatga ttccttgata
     4321 gcgttcggtt tctaaaagtt gcacggcatt cacacggaca agagaaagag aatttttaac
     4381 tttttgatga cggtgttcta aaaaaacacg agcacgagaa gcgttccata aagaagtatc
     4441 tgtactttgc ctatacccca tttcaacgcc agcaaaaaga tgtaaaggca aacgccattt
     4501 tatattcaag tgatcatgcc tcaaagcact cgttaacaag ctactatcaa gatcccattc
     4561 ttcatttaaa gagtaagatt cccaatctcg atctgttcct tgaaaaagag tattcaaaaa
     4621 acctgtttca taataatttc cataaagaga aatggcgatc gggaaatgaa tgagattcgt
     4681 ggatgagtca gaagataaaa cccagcgaga agctcttcca acaggtccat caacataatc
     4741 agaaacgata ttcgtatctt ttttttgata agccatttca gcatctaaaa accagcgtcg
     4801 atttgcttgt gctcgaattc tcgcactcat tcgatccgtt tgaacaggac gttctaaaga
     4861 gcctaaagaa tcactccaca aagccccatt tccagaatcc gcttttaatt tttcataatc
     4921 attttcagtc catgtgcctc ttttcaatcg ttccacatca ctggataatc taaaaccacc
     4981 gacatcaagc gaaatatttt ttgaacgata acgtccttca aaagccttta aagttgaaat
     5041 agattgagag gcataagaat catattctat acgaatttca tcttgagctc caggcaaatg
     5101 gatgccctta aaatcaagta ctccaccagc ataattcaaa tcataatcca cgtctttttt
     5161 taaagccgtc ccattcagcc aaaccttttc agaaccaggt acaatcgttt gataaagccc
     5221 atccgtattc acaatatacc ctgaacgctg cccatctacg ccattaaaaa catggacttc
     5281 tcgaaggact tcatccgtgc cataagcgcc tctaacttcc gtattagaat aacgtatagc
     5341 agctccaatt cctaaagaag acctttctaa tcctaataaa ccaaattcat cttgtttcca
     5401 agaaaagtcc cctaaatgaa gcgagacata aggtgattct actttaaaat aaatttgatc
     5461 gacttcttgc agcgtggctg tattttggtc tgcagggctt ctccctacat ccgaaagaag
     5521 ggcatctaca aaaaccgaag ggtacacttc tccttgaatg gaaagcctaa gttcttgatc
     5581 aacttgagtg cctccatctc caacaactac ttgtaacgtt ttagaaccct gagtttttaa
     5641 ttttaaggaa tctttttgga ctggagtttg agcttcatta tctatttgta tttcgttccc
     5701 tttccaaaca ggtaacgagt cgagattcaa agcccatgat gccaaatgaa agcagcatat
     5761 gatactgaaa ataaatccca aacgatcatg atttttcaaa atcacaatcc tttagattct
     5821 accttgaatt gacgagaagt ccatagtttc cgtccttgta caagatctac actccattca
     5881 cctgcacgta ataaatttgg agctatagaa ctgacacaaa tattctcttg ttctttacaa
     5941 ttagatacaa gcattgtatc tagacctata aaccaaattt gcttaaaggg aaccgaatct
     6001 atagagctgc cttctttttc gattggcaaa tgagaatgaa aatataatcg tgtattttct
     6061 tcgaaaatag aatctacacc aaaaggcgca ccatcgacaa tattacgaca taccacgctt
     6121 tgttctggag ataaaagaga aactgtttct accacaggtt caacgaaatc tgactttaag
     6181 ctcacataaa ttcgatgtac aaaaaaagaa aaaaacacca ccgcaataat cagtgttgtt
     6241 tttcttaggt aacgaagtgg cggaatacct tccacagaca cctcttatta aattaattta
     6301 cttgtatttt gtgtaaacgc aggaacagcg tctaccaatt tttcaactaa taaacgatta
     6361 taatcttcaa tacgattagg taacttagtg cctggaaata tttgcactaa aagcaaagac
     6421 tcattcactg gtgaaatata gatagagcga ttctctccac cataagacac ggattggaag
     6481 ttttcaccgc caagcatcat tcctatctgt ttagctgaat caaatgaagc cgtacttaac
     6541 acagccagtg gagttgcatc cacacctaga gctccaactt cagcgataat ggatccatcg
     6601 cgatgacaaa gaacgacata atctgctttt acacttcctt gataagcaag catcaaacgc
     6661 cgtactttct ctgcatcttc tgaatatact gcaaaatcgc tcataaaaaa acctcgttct
     6721 agttcaacac atcactctta cttcttagga acgtaagggc gtagttcaat atcatcttct
     6781 gaaacatctt gatttggttt tggaacctca cgaccaaacg tgggcattct ctgaggtgga
     6841 ggcgttccta cttttggacg accaaatgga gatgctaatg cacttttttg gaacaaacca
     6901 gcatttcccg ttcgaggtgg ttctggtgca ggtgcttttg ctgcaaatga tggcatctta
     6961 aatggagagt ttggtgcacc tgaactttgt agatctggtg ggcctgattt ggagtcttct
     7021 ttagccggaa tatctgtcat agaaacgcca tttttattta gcgttaaatc atgaacaccc
     7081 gcattattaa cgctctcagc ggccttacgt aaaaagcctt gctttacatt aaacttttca
     7141 atcacaagca tggcaatcat tttaagcgta gtcacgacac ctttgccatt atgtgctgta
     7201 gctgggaaaa atggaataga gcgtggattc aaatatttat tcatttcatc taatgtgaat
     7261 acatcgtcca tgtctctttt gttgtattga agaacaaaag gcatgtcatc taaatccatc
     7321 ccataatctt gaagattttg tcttaaattt tgcaagcttt ccaaattttc agcttggcgt
     7381 gaacgttgag aatcagcaac aaaaacaatt ccatctgcac cacgaagaac aagcttacgg
     7441 gtgctgttat aataaacctg accaggcact gtatatagtt gaaaacgagt tttaaaacct
     7501 ttaatatcac ctaaattcaa cggtaaaaaa tcaaaaaata acgtacgatc gccttccgta
     7561 gcgagcgaga ccatatccgt tcgtttatct tgcggcattt tctgatgaat cacctgcaaa
     7621 tttgtcgttt taccacttag accgggtccg taataaacaa ccttacaact gatttcgcgt
     7681 gttgcaaaat ttattgaaga catctctatt ccttcgtatt tacacctaat ctaactaatt
     7741 tctaacatct atgttttaat tattgaaaaa aaaaccaatg ttttgagcca ttttaaataa
     7801 gcgtaactta ttattaaaga atacgttacg actagctcaa attaagtata tatataaaaa
     7861 tcgacagtgt aaaacactgt cgacttatat ctctcaataa caaaagtgat taagattaag
     7921 ctttagcagc taggttacta ataaccttag atacttttgc tttataattt gctgcacgat
     7981 tagcagaaaa accagcacgg ccttttgcag ctgctttatc taacatactg aaaagatttg
     8041 gcatttcttt gacagcatct tctttagaag tggctgtacg gattgttttt aagctggtac
     8101 gaatcgcaga acgaacggaa cgattgacag cattagcttt ttcagcttga cgtaaacgct
     8161 ttttacaaga taaatgatta ggcaccttaa aatctccggg tgaaaaccta tacttaattt
     8221 tattgccgca agtttagcat ttattctaaa atcagtcaag gtcacataat ccaaaaagct
     8281 gatttttcta aaacttgcaa taaaaaacga gtaaaagggc ctaaaatgaa agataatggc
     8341 cctggaaatg ggaaaaacca ctcccaaacc acaaaaaata ataaaggcca agagccaaat
     8401 ttaatgaaaa aggcatcaaa ttgacgactt aaagtgggag gtaaaagcga agaaaccact
     8461 ttcgctccat ctaaagggta aactggaagc aagttaaaag cagttagagc aatatttaca
     8521 agcacaaaaa cccttagaaa atcaatgatt aatcgctcta tattcacgtg taagcctata
     8581 ttcgggagta atatggcttg aaaaagcagg aataattgaa agcctaatac cgctaaaatc
     8641 agattcgata aagggcctgc taaagagact aaaccgatgt aacggctctt tttcaaggag
     8701 ttcgtgtcca cagggactgg ttttgcccaa ccaattccca tcccttgaaa agccataaac
     8761 gcaatcatga ttgttccaag cgtatctaaa tgcaccaagg ggttcaatgt taagcgcccg
     8821 cgatccttcg ccgtcgaatc gcccaaaaca taagccatca aggcatgagc ggcctcatga
     8881 aacgcaagag ataaaacaat cgctaaaaaa taaatggtac ctaaacgtaa agactcactt
     8941 cccatattca ttccatgacc ttaataggcc tcaaaaggtt ccgtttcaaa caaagaggag
     9001 ccataacgca actctgtctt tgttttccat agataaacat tgccaagcgc cccttttaag
     9061 atggcgtggc ctgccgcaat atcccactcg tgcaattttc ttaatctagg ataataattt
     9121 gcggtaccat cggcaattcg acaaaatttg agactagaac caataggtaa aaccgttcca
     9181 cctagcgaag atacatattc ttccgttggc tgatcaaaat gcaacacact cacaagaatt
     9241 ttagactctg gattcatttg atttagatcc caagattctt ttattaagcg acggctttga
     9301 aaatcttctt tattccatag tggagtaacc caagactctt gggtgttacc aacaaacagt
     9361 tctccagtca ctggaacggc aacaaaacca agaacggcaa ggccgttttc tatcaaagcg
     9421 atatttacgg tgaaatcacc ttgatgcgcg acaaattcac tagttccatc aatgggatca
     9481 atcaagaaaa aacgattcca agaggaacgt tcttctatcg aactattttg aatttcttca
     9541 gatatgatgg ggatttcggg gtaatattct cgtaagccgt ccataatgat ttgattcgct
     9601 tctaaatcgg cactcgtaac aggagtggcg tcttcttttg aagaaacggt aaagtcactt
     9661 ttataggaat ctaaaacggc ttggctagcc ttgcgcatga tttcaaatat aggggcccat
     9721 tcagcaaaag tgttcatagc tataagataa aaaatgtttt gtctgcatag cgtatgtttt
     9781 tttcattacg attaaatcta tatagccaat gttggcggca atggtttttt cattacgata
     9841 tgattctata taaccattat tgacggaaat gattttttca ttaaaactca ataatactat
     9901 ggttgacggg catggctatt acctcct
//
LOCUS       DAOOBB010000058         9853 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_8582_length_9853_cov_4.507553, whole genome shotgun sequence.
ACCESSION   DAOOBB010000058 DAOOBB010000000
VERSION     DAOOBB010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 9853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9853
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8582_length_9853_cov_4.507553"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="PLT31_04125"
     CDS             complement(<1..663)
                     /locus_tag="PLT31_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW94357.1"
                     /translation="MKAIEIKDMSFSYGNTPVLDQVTFSVEEGDFLGIIGPNGGGKTT
                     LMKLILGLLKPTSGSIRLFGKKVPSHKIKVGYVPQNTNYNIEFPITVEECVSLGLVGE
                     KKNKETIHHALERVQMIDYRAKRLGELSGGERQRVLVARSLVSSPRILFLDEPSSNID
                     SAGQENLFQLLYDLNKTMTLVVVSHDLMVLSNHIKSVACVNRTVHYHPESEITEDMME
                     SMY"
     gene            complement(669..1586)
                     /locus_tag="PLT31_04130"
     CDS             complement(669..1586)
                     /locus_tag="PLT31_04130"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HPW94358.1"
                     /translation="MNPFYLAIVLLCAFGSVASASKLNVAVTLQPYAKIVEEIGGDRV
                     SVISMIPPNADPHSYEPKPGVLKTFSKADLYLSDSSGLDQVWLPRFLSVNKKIEMVYI
                     SSGVSWMQESEDHHHGHHHDHHHHGEDHHENKHKHEENASLDPHLWTSPKQVIKLAEN
                     VLSALIKKDAASKSYYESRYQLFIEKWTSIDQFIEKTVSQIPEKRRIFIVFHPSYGYL
                     ARDYDLIQRAIEVEGKEPKPQDLKALILEAQKNDIRAIFVQPQFSKRSAESIAKQLNA
                     VVVSTDPLAYDIEKNLRNFIQALLEASKK"
     gene            complement(1583..2389)
                     /locus_tag="PLT31_04135"
     CDS             complement(1583..2389)
                     /locus_tag="PLT31_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HPW94359.1"
                     /translation="MLENKKPVRAVVFDLDGTLYLSGKPYPKAVETVNRVAKQVPVYY
                     LSNNTSKSPVFYENRLLRMGLPLFDGAIISALSLALQAIQREHIKNLYFFANPEVFEW
                     FAAQDSSLNLKSSVEDAELVLMAYHNSFDYEELCEVSWRIQRKTRWWVTHSDFVCPDA
                     RGPVPDIGSFMALFERAYGVKPEKSFGKPNPEMLAPVLERFAPDEVLFVGDRLYTDFE
                     LAKQSGCRFALPLCGETKEKDLWGLSVLPEFIVDQVSDINFDSLIEGIQK"
     gene            complement(2376..3257)
                     /gene="rfbD"
                     /locus_tag="PLT31_04140"
     CDS             complement(2376..3257)
                     /gene="rfbD"
                     /locus_tag="PLT31_04140"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HPW94360.1"
                     /translation="MKLKIWITGASGMLGKDLTSSAKAQGHECLLTDLDVDITDEKAV
                     TLFLENNKPDRIVNCAAYTAVDLAETETALNYKVNAIGPQVLGKCAAQKKVPLLHIST
                     DYVLNGNPPAPLNPEDEYHPVNAYGKAKMEGERFLLESGASVWIIRTAWLYGINGKNF
                     VKTMLSLMNTKEKLTVVNDQFGNPTWTVDLARAILTVLQSGSHFGIYHFTGEGITTWC
                     DFALKIQELGLQEGLLSKKIPIEGVLSSQFKTAAVRPSWSALSKEKIKSTFSIQVPKW
                     EDSLASYIQLEKEHAGK"
     gene            3347..4057
                     /locus_tag="PLT31_04145"
     CDS             3347..4057
                     /locus_tag="PLT31_04145"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HPW94361.1"
                     /translation="MRSPDSHFFHSPLSVGELILNEEESTHAIRVCRAQKGDFLRLCD
                     GEGHFAKGIILETDPKECRVLVENIETASGEKPRFSLALACLKDDGNEEVVFHASQTE
                     LNQIVFLRTDYSQEPKNSDCKKIIRRAELKSKVSLKQSLKPWLTAIQGPIVLDDFLAN
                     YSGDLILADIDGDPELKPLNENVETTLLVGPEGGFSAREIELIKAYSRGQVYLLNLGK
                     TRLRAKTAAIIALGKCLH"
     gene            complement(4376..5260)
                     /locus_tag="PLT31_04150"
     CDS             complement(4376..5260)
                     /locus_tag="PLT31_04150"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPW94362.1"
                     /translation="MKNVAIIGASGFVGPYLVQEFTQAGYSVFPVDLPEVDILKRETL
                     DAFFTKVKPDFVVNLAAISSVGASWNNPGLTVQVNVNGSLNVLDSLVSVGLLSSRILL
                     VGSSEQYAPCDRPILETDPLLATNPYGVSKIAQENFSSLYQKKYGLKIINTRSFNHTG
                     VGQSDQFVIPSFCKQVAEIDKRGVPGKIAVGNLSAYRDISDVRDVVHVYRMLLEEDQT
                     ESIFNVGSGKVHRIKDLLDYILSLSSCKIEVIEDPARMRPVENPYLCCNPSRIERYWG
                     GRDLKDTIREMFLYFKEH"
     gene            complement(5257..6279)
                     /gene="gmd"
                     /locus_tag="PLT31_04155"
     CDS             complement(5257..6279)
                     /gene="gmd"
                     /locus_tag="PLT31_04155"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPW94363.1"
                     /translation="MTKALITGITGQDGSYLAEFLLEKGYEVYGLVRRKSKLDFGNAC
                     HLQGRVKFIFGDMTDLASLIRAIEISTPDEVYNLAAQSFVTASWETPLSTADINSIGV
                     TNILEAIRLIRPKCRFYQASTSEMFGKVQEIPQNEDTPFYPRSPYGVSKLYGHWITKN
                     YRESYGMYACSGILFNHESERRGAEFVTRKITIAVAKIKHGLQDVLELGNLDAKRDWG
                     HSKDYVEAMWLMLQQEVADDFVVATGDTHTVREFVTLAFKAAGMHLDWHGEGSKEYAT
                     LKETDRVVVRVNPEFYRPAEVELLIGDPSKAEKLLGWKRAISYNDLISRMVGNDLQLV
                     EKSLKK"
     gene            complement(6312..8549)
                     /gene="pflB"
                     /locus_tag="PLT31_04160"
     CDS             complement(6312..8549)
                     /gene="pflB"
                     /locus_tag="PLT31_04160"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: protein motif:HMM:TIGR01255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HPW94364.1"
                     /translation="MIEKNAWRSFVSGSWDSEIDVKDFIQKNRTPYEGDDSFLAKATA
                     RTQKLMESVNALLKEERKKGILGASVDVGTSITAHKPGYIDQALEKIVGLQTDAPLKR
                     AIAPNGGLRMVETGLEDYGYTIDPLIKEVYTKYRKDHNRGVFDVYSPDIRACRKSGVI
                     TGLPDAYGRGRIIGDYRRVALYGVAFLIEAKEKEKAELDYADFTEDVIRLREELSEQI
                     AALKELVEMASSYGFDLLKPAENAIESIQWTYFAYLAAVKEQNGAAMSIGRISTFLDI
                     YIERDLAEGTITEVDAQELIDDLVIKLRLVRFLRTPDYDALFSGDPTWVTESIGGMSE
                     DGKTLVTKSSFRFLHTLYNLDPAPEPNLTVLWSEHLPSHFKEFCSKVSIDTCAIQYES
                     DDLMRPRWGDDYAIACCVSAMKIGKQMQFFGARANLAKTLLYAINGGVDEKLFIKVAP
                     GFEPITDEVLDFDKVMEKYDLMMEWLAHTYVKALNAIHYMHDKYYYERIEMALHDRDI
                     LRTMACGIAGLSVAADSLSAIKYAKVYPIRNEMGIAIDFKIEGTFPAYGNNDDRVDSI
                     ATMLVTRFMEKLKKQKAYRNSLVTQSVLTITSNVVYGKKTGNTPDGRRSGEPFAPGAN
                     PMNGRDVSGFVAAGASVAKISYDDALDGISWTATSTPEALGRTAKERIDNLSNCLDGF
                     VRAGGYHVNVNVLHKETLLEAMEHPEKYPSLTIRVSGYAVNFIKLTKEQQLDVIQRTF
                     HASLV"
     gene            complement(8546..9727)
                     /gene="purT"
                     /locus_tag="PLT31_04165"
     CDS             complement(8546..9727)
                     /gene="purT"
                     /locus_tag="PLT31_04165"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HPW94365.1"
                     /translation="MAIIGTPLSESATRVLFCGAGELGKEVIIEMMRLGVEVIAVDRY
                     PNAPGMQVAHRSHVINMLDGAELRRIIELEKPDYIVPEVEAIATDTLVEMESEGYCVI
                     PTAKATKLTMNREGIRRLAAEELHLKTSPYRFAETFESFSKAIHEVGIPCVVKPVMSS
                     SGHGQSVVRSESDIERSWNISQQEGRTGAGRVIVEGFVPFDYEITLLTVRHVGGTSFL
                     EPIGHHQVDGDYQESWQPQPMESHLLEQAKEMALRITDALGGRGIFGVELFVCKNEVL
                     FSEVSPRPHDTGMVTLISQDLSEFALHARAVLGLPIPNIAFHGPSASKAIVCDGQSKQ
                     LKFSHLEDVLAEKDTAIRLFGKRELKGHRRMGVLLARRQNVEEALDVVKKMREKVVVE
                     L"
BASE COUNT         3114 a         2094 c         1752 g         2893 t
ORIGIN      
        1 atacatactc tccatcatat cttcagtaat ctctgattca ggatggtaat gcaccgttct
       61 attgacgcat gccacacttt taatatggtt tgataaaacc attaaatcat ggctcaccac
      121 aacaagagtc atcgttttat taagatcata aagcaactgg aataaatttt cttggcctgc
      181 agaatcaata ttactagatg gctcatctaa aaataaaatt cgtggagaag acactaaaga
      241 tcgagcaact aaaacgcgct gccgttcgcc gccagagagt tcgcctaaac gcttggctct
      301 ataatcaatc atctggactc gttctagagc atgatggatt gtttctttat ttttcttttc
      361 gccgactagc cctaaagaaa cacattcttc tacggtgatt ggaaattcaa tattatagtt
      421 cgtattttga ggaacgtagc cgactttgat tttatggctc gggacttttt ttccaaaaag
      481 acgaatactc ccagaagttg gctttaaaag cccgagaatc aatttcatga gagtcgtttt
      541 accgcctcca ttagggccta tgattcctaa aaagtcacct tcttctacgg aaaaagtaac
      601 ctgatcaaga acaggtgtat taccataaga gaaagacata tctttgattt ctattgcttt
      661 cattagatct actttttaga ggcttctaaa agggcttgaa taaaatttct taagttcttt
      721 tcgatatcat aagctaaagg atctgtagag actaccaccg cattcaattg ctttgcgatg
      781 gattctgcag aacgtttact aaactgaggc tgtacaaaaa tagctcgaat atcatttttt
      841 tgagcttcaa gaatcaaggc tttcaaatcc tgcggcttgg gctctttgcc ctcgacttca
      901 atcgctcttt gaattaagtc ataatcacga gctaaatagc cataagaagg gtgaaacaca
      961 ataaagatcc gacgtttttc agggatttga gacaccgttt tttctataaa ctgatctatg
     1021 gatgtccatt tttctataaa aagttggtat cgagattcat aataagattt agaagcagca
     1081 tcttttttga tcaaggcaga aagtacattt tcagccaatt taatcacttg ttttggggat
     1141 gtccaaagat gagggtctaa agaagcattt tcttcatgtt tgtgtttatt ttcatgatgg
     1201 tcttcgccat gatgatgatg gtcatgatga tggccatgat gatggtcttc tgattcttgc
     1261 atccaagaaa caccgctaga aatataaacc atttctattt ttttatttac agaaagaaat
     1321 cgcggtagcc atacttgatc taagccagaa ctatcactta aataaagatc ggctttagaa
     1381 aatgttttta agactcctgg ctttggctca taagaatgag ggtctgcatt tggaggaatc
     1441 atgctaatga cagaaacgcg atcaccacca atttcttcga ctatttttgc gtaaggttga
     1501 agagtcacgg ccacattcaa tttagaggcc gaagcgaccg aaccaaaagc gcataataat
     1561 acaattgcta aataaaaagg attcattttt gaataccctc tattaaagaa tcaaaattaa
     1621 tatcactcac ttgatccaca ataaattcag gcaaaacact taagccccat aaatcttttt
     1681 ctttagtctc tccacaaaga gggagtgcaa aacgacaacc tgattgcttc gctaattcaa
     1741 aatcggtgta caaccggtcg cccacaaata acacctcatc tggagcaaat cgttcaagca
     1801 caggcgcaag catttcagga tttggtttcc cgaaactctt ctctggcttt actccgtagg
     1861 ctctttcaaa taaggccata aagctcccga tatcaggcac agggcctcgc gcatctgggc
     1921 atacaaaatc agaatgagtc acccaccaac gcgtttttcg ttggatacgc caagagactt
     1981 cgcaaagttc ttcataatca aaagaattat gataagccat caaaacaagt tctgcatctt
     2041 ctacagaaga ttttagatta agagaagaat cttgtgcagc aaaccattcg aatacttctg
     2101 gatttgcaaa gaaataaaga ttcttgatgt gttcacgttg aattgcttgt aacgcaaggc
     2161 ttaatgcaga aataatcgcg ccatcgaata agggtaatcc catgcgaagc agacgattct
     2221 cataaaaaac aggcgattta ctcgtgttat tactgagata ataaacaggc acttgttttg
     2281 ccacgcgatt gacagtctcg accgctttag ggtatggctt tccgctcaag taaagcgtgc
     2341 catctaaatc aaaaacaact gcccgaactg gttttttatt ttccagcatg ctctttttcc
     2401 aactgaatgt aagaagctaa agaatcttcc cacttaggga cttgaataga aaaagtggat
     2461 ttaatctttt ctttagagag cgcagaccaa gatggacgaa ccgcagcagt cttaaactgt
     2521 gagctcaaaa cgccttctat agggatcttt ttggaaagca aaccttcttg aagccctagt
     2581 tcttgaattt ttagagcaaa atcacaccaa gtagtgatcc cttctcctgt aaaatgatat
     2641 atcccgaaat ggctgcccga ttgaagcacc gttaaaatcg cacgcgctaa atcaactgtc
     2701 cacgtagggt taccaaactg atcattcacc acagtaagct tctcttttgt attcattaaa
     2761 gagagcatgg ttttcacaaa atttttacca ttgatcccgt agagccatgc ggttctaata
     2821 atccataccg atgccccact ttctaataaa aagcgttccc cttccatttt tgcttttcca
     2881 taagcattca caggatggta ttcatcttca gggtttagag gggcaggagg attaccgttc
     2941 aacacataat ctgtagagat atggagtaac ggcacttttt tttgagcggc acatttgcct
     3001 aaaacttgtg gcccaatcgc attgacttta taatttaagg cagtttctgt ttctgctaaa
     3061 tcaacagcag tataagccgc acaattaacg atacgatctg gcttattatt ttctaaaaaa
     3121 agagtcacag ccttttcgtc agtgatatcg acatcaagat ctgttaatag gcattcatgc
     3181 ccttgcgctt ttgctgagct tgttaggtct ttccctaaca taccgcttgc tccagtaatc
     3241 catattttta acttcatatt taaaatttag taaaagttct tattcaaggt gaacaattga
     3301 atgaatcacc ctctatttaa gctaaaaaga attttatctt taacaaatga gatcaccaga
     3361 tagtcatttt tttcatagtc cgctttctgt tggcgaatta attctcaacg aagaagaaag
     3421 tacccacgcc atacgcgttt gtagagctca aaaaggcgat tttttacgtt tatgtgatgg
     3481 tgaagggcat tttgctaaag gaattatttt agaaacagac ccaaaagaat gccgcgtttt
     3541 agtagaaaat atcgaaacgg cttctggcga aaagcctcgt ttttctttag ctctcgcctg
     3601 tttaaaagac gacggcaacg aagaagtggt gtttcatgcc tcacaaacag aattaaatca
     3661 aattgtattt ttaagaaccg attattctca agagcctaaa aattcggact gtaaaaaaat
     3721 aatccgtcgc gcggaattaa aaagcaaagt gagcttaaaa caatcactaa agccttggct
     3781 caccgccatt caaggaccaa ttgtacttga cgatttttta gcgaattatt ctggcgatct
     3841 tattttagcc gatattgatg gcgacccaga actaaagcca ttaaatgaaa atgtagagac
     3901 gactcttctc gtagggcccg aaggtgggtt ttctgctcgt gaaatagaat tgattaaagc
     3961 ttatagtcga gggcaagttt atttattgaa tttaggcaaa accaggctta gagcaaaaac
     4021 agcagcaatc attgccttag gcaaatgttt acattaaaaa aattagtgtc ctattttttt
     4081 ggcaattatt ttgcggcttt aaagaaatga tttaatacga aataattttt ttggaaaaaa
     4141 atgtgaataa tattttcgcg gagaattaat tcagcaaaat cgttgtaaaa ataaacatga
     4201 gccatatttc caaaaatcgt tgtgaataaa ggtttcgcgg aaaatccatt tcaaaaaaca
     4261 tggtaaaaag agagaaaacc aattcgtaaa aattggtatt aatacaacat taaaggtatt
     4321 aatttaacgc gagaattgtc gctctaaaaa ccgttaattt atatgtaaag atttcttaat
     4381 gttctttaaa ataaaggaac atttcccgaa tagtatcttt caagtctcgc ccaccccaat
     4441 aacgctcaat ccttgaagga ttgcaacaga gataaggatt ctctacagga cgcattcgag
     4501 ctggatcttc tatcacttct attttacaag aagaaagaga caagatatag tctaataagt
     4561 ctttaatacg atgcaccttg cccgaaccca cattaaaaat agattccgtt tgatcttctt
     4621 ctaaaagcat gcgatacaca tgaacgacat cgcgtacatc ggaaatatct ctgtaagcag
     4681 ataaattacc aaccgcgatt tttcctggaa cacctcgttt gtcaatttca gccacctgct
     4741 tacaaaaaga agggattaca aattgatctg actgccctac tcccgtatgg ttaaaagaac
     4801 gagtgttaat gatttttaga ccatattttt tctggtataa agacgaaaaa ttttcttgag
     4861 ctattttaga gaccccataa ggatttgttg ccaaaagagg atctgtttct aaaataggac
     4921 gatcacaagg agcgtattgt tcgcttgaac ccacaagtaa aatacgagat gataagagcc
     4981 ctacggaaac taaagaatcc aaaacattca aagaaccatt gacattcact tgaacggtta
     5041 agcctgggtt attccaagag gcgcccacag aactaatggc cgctagattc acgacaaaat
     5101 caggttttac ttttgtaaaa aaagcatcta gcgtttctcg ttttaaaata tcaacttctg
     5161 gaagatccac aggaaaaaca gaataccctg cttgagtgaa ctcttgaacc agataagggc
     5221 caacaaaacc actggcccca ataatcgcta catttttcat ttttttaagg atttttctac
     5281 tagttgcaaa tcgttaccaa ccattcggct aatcaaatca ttatagctaa tggctcgttt
     5341 ccagccgagt aatttttctg ctttagaagg atctcctata agcaattcta cttctgctgg
     5401 gcgataaaac tctggattca cacgaactac gacgcgatcc gtttctttta atgtagcgta
     5461 ttctttggat ccttcaccat gccaatctaa atgcattcca gccgccttaa aagcaagagt
     5521 cacaaattca cgtacggtgt gagtatcgcc tgtggcaacc acaaagtcat cagcgacttc
     5581 ttgctgaagc attaaccaca tagcctccac ataatcttta gaatggcccc aatctctttt
     5641 cgcatccaaa tttccaagct ctaaaacgtc ttgtaatcca tgtttaattt tggcgacagc
     5701 gatggtgatt tttcgagtga caaattcggc accacgacgc tctgactcat gattaaaaag
     5761 gattccagag caagcgtaca tcccgtaact ttctctataa ttcttggtaa tccaatgccc
     5821 gtaaagctta gatacaccat aaggacttct tgggtaaaat ggagtatctt cattttgagg
     5881 aatttcttga acctttccaa acatttcact tgtagacgct tgataaaagc ggcatttagg
     5941 gcgaatcaaa cgaatggctt ctaaaatgtt ggtcacaccg atcgaattaa tatcagcagt
     6001 agataaaggc gtttcccaag aggccgtgac aaaagattga gcggctagat tatagacttc
     6061 atctggagtc gaaatttcaa tcgctcgaat tagagaagcc aaatcggtca tgtctccgaa
     6121 aataaatttt actcgaccct gtaaatgaca agcattacca aaatctagct tactctttcg
     6181 gcgcactaag ccataaacct catacccttt ttcaagaaga aattcggcta ggtaggagcc
     6241 atcttgacct gtaatacctg tgattaatgc ttttgtcatt cgaatccctt ttagattttt
     6301 agaaccattt cctatactaa agacgcatgg aacgttcttt gaatcacatc taattgttgc
     6361 tctttagtca acttaataaa attcacggca taaccagaaa ctcgaatagt caaagaagga
     6421 tacttttcag ggtgttccat tgcctctaac aaggtttctt tatgtagtac attcacattc
     6481 acgtgataac caccagcacg cacaaatcca tccaaacaat tcgaaagatt atctattcgt
     6541 tctttggcgg ttcgaccaag agcttctgga gtcgatgtag ctgtccaaga aataccatct
     6601 aaagcatcat cataagaaat ctttgccaca gaagcgccag cagcgacaaa gccagagaca
     6661 tccctaccat tcataggatt tgctcctggt gcaaaaggct ctccactgcg acgtccatct
     6721 ggagtatttc cagtcttttt gccataaacg acattagaag taatagtcaa tacagattga
     6781 gtcactaaac tatttcggta tgccttttgt tttttgagct tttccataaa acgagtcaca
     6841 agcatcgtcg caatcgaatc gactcgatca tcattatttc cataagcagg gaaagtccct
     6901 tcaatcttaa aatcaatcgc gatacccatt tcattacgaa taggatacac ttttgcatac
     6961 tttatggctg ataaagaatc ggcagccaca gaaaggcccg cgatgccaca agccatcgtg
     7021 cgaagaatat ctcgatcatg caaagccatt tcgatacgtt cataataata cttatcatgc
     7081 atataatgaa ttgcatttaa agccttgacg taagtatgag caagccattc catcatcaaa
     7141 tcatactttt ccatgacctt atcgaaatca agaacttcat cggtaatggg ctcaaaacct
     7201 ggagcgactt tgataaagag cttttcatct acgccaccat tgatggcgta caatagagtc
     7261 tttgctaaat tcgctcttgc tccaaaaaac tgcatttgct ttccaatctt catcgcagaa
     7321 acgcagcaag caatggcata atcatcgccc catcttgggc gcattaaatc atcggactcg
     7381 tattgaatgg cgcaagtatc aatagaaacc ttagaacaga attccttaaa atgagaaggc
     7441 aaatgttctg accaaagcac cgttaaatta ggctctggag cagggtctaa gttataaagc
     7501 gtatgtaaaa aacggaaact actttttgta acaagagtct taccatcttc actcatgccg
     7561 ccaattgatt ctgttaccca agtagggtct ccagaaaaaa gagcatcata atctggtgtt
     7621 cgtaaaaaac gaaccaatcg caatttaatc accaagtcat caattaattc ttgagcatcg
     7681 acttctgtaa ttgtgccttc agctaaatcg cgttcaatat agatgtctaa gaatgtcgaa
     7741 atacgaccaa tagacatcgc ggcaccattt tgttctttaa cagcagctaa gtaagcaaaa
     7801 taagtccatt gaatggattc gattgcattt tctgcaggct ttaacaaatc aaaaccataa
     7861 gaagaagcca tttccactaa ttctttaagt gctgcgattt gttctgaaag ttcttctcta
     7921 agacggatca cgtcttcggt aaaatcagca taatcgagtt ctgctttttc tttttctttc
     7981 gcttcaatta aaaaggcaac accataaaga gcaacacgac gataatcacc aatgatacgg
     8041 ccacgcccat aagcatctgg aagccctgta atcacaccag acttacgaca agcgcgaata
     8101 tctggactat acacatcaaa gacgcctcga ttatgatctt tacgatactt ggtgtacact
     8161 tctttaatca atggatcaat cgtgtaacca taatcttcaa gccctgtttc gaccatacga
     8221 aggccaccat ttggagcaat cgcccgtttt aatggagcat cggtttgtaa gcctacaatt
     8281 ttttcaagag cttgatcaat ataacctggt ttatgagccg taatcgaagt acctacatcg
     8341 acacttgccc ctaaaattcc ttttttcctt tcttctttta agagagcatt aactgattcc
     8401 atcagttttt gagtgcgcgc ggtggcttta gccaaaaatg aatcatctcc ctcataagga
     8461 gtcctatttt tttgaataaa atctttaaca tcaatttcag aatcccatga tcctgaaaca
     8521 aaagagcgcc atgcgttttt ttctatcata attctacaac taccttctcg cgcatttttt
     8581 tgactacatc aagagcttct tcaacgtttt gtcgacgagc aagtaaaaca cccatacgac
     8641 gatgtccctt tagttctctc tttccaaata aacgtattgc tgtatctttt tcagcaagaa
     8701 catcttctag atgactaaac tttaattgtt tcgactgacc gtcacataca atggctttag
     8761 atgccgaagg accatgaaag gcaatattgg gaataggtaa acctagcacg gcgcgagcgt
     8821 gtagggcgaa ttccgataaa tcttgagaaa ttaatgtgac catccccgta tcatgaggtc
     8881 gaggactcac ttcgctaaat aacacttcat ttttacagac aaaaagctcg acaccaaaaa
     8941 tgccgcgccc acctaaagcg tctgtaatcc gaagagccat ttcttttgct tgctctaata
     9001 aatgagattc cattggctga ggctgccagg attcttgata gtctccatcg acttgatgat
     9061 gaccaatagg ttcgaggaaa gaagtgccgc ccacgtgacg aacggttaaa agagtgattt
     9121 cataatcaaa aggcacaaaa ccttctacaa taactcgacc agctccagtc cgaccttctt
     9181 gttgagaaat attccaagaa cgttcaatat cgctttctga acgaaccacg ctttgcccat
     9241 gaccagaaga actcattaca ggtttcacca cgcaaggaat ccctacttca tgaatcgctt
     9301 ttgagaaaga ttcaaaagtt tctgcaaatc ggtaaggaga tgtttttagg tgaagttctt
     9361 ctgcagctaa acgacgaatt ccctctcgat tcatggtgag tttagtcgct ttagcggttg
     9421 gaatcacaca atacccttct gattccattt ctacaagagt gtctgtggca atggcttcta
     9481 cttctggaac aatataatca ggcttttcaa gttcaatgat acgacgtagt tcagcaccat
     9541 caagcatgtt aatcacatgg gaacgatggg ccacttgcat gcctggggca tttggataac
     9601 gatctacagc aatcacctct acaccgaggc gcatcatttc aataatcact tcttttccaa
     9661 gttcacccgc tccacaaaaa agaactcgag ttgcagattc acttaaagga gtaccaatga
     9721 ttgccataaa acctcttttt ttaacaaaaa cgttggtatt tcaaaccaat ttatcactta
     9781 cgtttttaca agatatggtg cactattagg ttaaagagat actacatctt gtttcactaa
     9841 aatagaaaaa aaa
//
LOCUS       DAOOBB010000059         9690 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_8742_length_9690_cov_4.842761, whole genome shotgun sequence.
ACCESSION   DAOOBB010000059 DAOOBB010000000
VERSION     DAOOBB010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 9690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9690
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8742_length_9690_cov_4.842761"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..765
                     /gene="rsfS"
                     /locus_tag="PLT31_04170"
     CDS             133..765
                     /gene="rsfS"
                     /locus_tag="PLT31_04170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HPW94366.1"
                     /translation="MNEKKSTRKPSVKKATTPVKTATVKKEAAKKTVVKKEAAPKATS
                     KKAPAKKVATTKTVTKAVKTVKAVKATPAKKKEALSEAVSLAAVSLFQLRAENVQLID
                     LRGINDVADYFLIATCTSEAQMQAILNELTKEFKSKKIQTKGIEYKSGVQWAVFDAGF
                     ELMVHLFEETKREEIQMDRLYSDANIQNLQEKDFIKKTTKKKANIEDDLI"
     gene            752..2467
                     /gene="argS"
                     /locus_tag="PLT31_04175"
     CDS             752..2467
                     /gene="argS"
                     /locus_tag="PLT31_04175"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPW94367.1"
                     /translation="MISFEQSIAAAVAKTGTFLEEEVLQLLTVPQETTHGNFSLPCFA
                     LAKKLRKAPKIIAETLATEIELPQGISKVEPINGYLNFFIERSFLATSVLTQIEEKGL
                     NYGHASSNNKTVIIDFSSPNIGKELAFHHLRSTMIGNSLSRIYKANGYRVERINHLGD
                     WGTAFGKLIVMYLREGGSLEEEALDQLTVKELNLLYSSFSKISKENPELENEARAAFT
                     KLEEGDSLYRKLWSAFKKATLKELMRIYSMMGVSFDHYIGESFFEDKIPAVLDELREK
                     KLLVKSQELDVVMLNDFDLPPCLIRKSDGSTLYATRDLAAALYRKKEFQFSKCLYVVD
                     LGQSLHFKQVFHVLKKMDYEWYQDMYHIPFGVILQQTEEGKWEKGKTRSGTASLLRDV
                     IEAAKEKILTFINEKNPELENKEEIATQIGISALTFNDLKNRRLMDVKFDWDSALSFD
                     GATGPYVQNAHVRLCSIMRKAAYTAKIEDVSWDTLTDDAAYELIKVLSRKGNNIAKAC
                     ETDEPSTLAQYALEIAEAAHRFIHQDRVLGSAEEKSRLYLVQATQIVLENVLDLLGLF
                     PIRQM"
     gene            2871..4424
                     /locus_tag="PLT31_04180"
     CDS             2871..4424
                     /locus_tag="PLT31_04180"
                     /inference="COORDINATES: protein motif:HMM:NF027224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR-4 repeat-containing protein"
                     /protein_id="HPW94368.1"
                     /translation="MHLLFLILFIAISLHAAVVERSEISSVWSSHFVDFAIATDSTAK
                     LQYVSFYDKDRKMTIAMRSLSSNDWQFKTLPQTTGWDSHNYIAMAVDDSGYIHISGNM
                     HNAPLVYFRSSKPHSIDNFESLSMTGSNETKVTYPIFIKGIEKGELFFQYRDNANNTS
                     TTIWNKYSTATKTWTRLTTQGFFNTEGLYSAYMTTPIKGPDDYFHVVWMWRNTPVANT
                     NHDISHIRSKDLVHWETMSGLDVTLPIKQSTPGVIVDSVDAGNGLINIAFGIGWDNQN
                     RAVINYHKYDVNGISQRWNTRWENGSWKIYQTSSWNSFKWPLDRNGTLGIDISGSPVK
                     KDYSGRLVQEYSHKNFGNRMWVLNEETLQPEKDTLPIIIPGLDSLYQVKSTFPEMQVH
                     FKVDGDYYLRWETLPENQDLARNPPLPEPTMLSVYKIDSKDPTQKLFSMKEEKSYPTF
                     VQNSTLTINGLNENASVDLYTLKGKKISASKHTSSIQEFSNLARGTYLYIIRNGEALT
                     PVINKVFIK"
     gene            complement(4594..4923)
                     /locus_tag="PLT31_04185"
     CDS             complement(4594..4923)
                     /locus_tag="PLT31_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HPW94369.1"
                     /translation="MAIEQFADILLEKLRFISQAETVVGKPIQAGEHTTIIPVSRVSL
                     GFGLGGHSGKGDITGSGGGASVEPVAFLVISGEDVRIVPVSRDNSMLSKIVDLVPDVV
                     SSFKKNP"
     gene            complement(4954..5775)
                     /locus_tag="PLT31_04190"
     CDS             complement(4954..5775)
                     /locus_tag="PLT31_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94370.1"
                     /translation="MNRLALISMVLSFIIACSDEGIPLRSVSFDSIVYQNHKHESAKW
                     VKQDSSKWHLLEEIHLLYKMDLDDSLWVSILEFENDAFALAYYINSGRFQGALPVVKG
                     PLLEQSIRSGRWLFIFRHKRFYPHDRRLLEEYVQQFPGYRGGLPQEFLSLPFENREPG
                     KTSVQIDLFHGEKISFPMFVQRYRNADVYWSVARSFESVDNEDWLKWVDSISKKKEKV
                     SYTESILKFDSGHGTRAMATRLGGGRVVCVWGPLDSKKLETLFFEISERVYSAKY"
     gene            complement(5780..6601)
                     /locus_tag="PLT31_04195"
     CDS             complement(5780..6601)
                     /locus_tag="PLT31_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94371.1"
                     /translation="MSKQGLRFARENPHIVEAARSFILEFDSVPDPVVKVASLAEDEN
                     SQIAWVLLGSVIFQNLSFFQMASVLEALFNAFPNKKLWETPVPKASDIKAVVQKALKK
                     ESWALLDHAPGMFWSVGFFIRRHFPLNIWLTSRNEAEIWRDLGEIYFMGKKNIRPKVM
                     SAIHRLKSNSPLGLGLTIREGKSKLPFPISMGSRRFMGFIGPGKEDDFANWTPEKKII
                     EVNDFYKILSPMAIDKAAHGLSFFLEPGEKDFLCREQIKSCEKCPLYSECSYGRP"
     gene            complement(6601..7458)
                     /gene="asd"
                     /locus_tag="PLT31_04200"
     CDS             complement(6601..7458)
                     /gene="asd"
                     /locus_tag="PLT31_04200"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545364.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HPW94372.1"
                     /translation="MNTPFYIFMLLLPKSLVSQVFGIFTRLRLPGISAKARDWFASHY
                     QLNEEESEKPFSEYPNIATLFIRHLKKGARPIDPSAEVVSPVDGVLSQTGVFDNNSQE
                     MIQAKGKNYDLASLLRSEEMAKDFEGGFWATIYLAPFNYHRIHTPVSGSIVSAHYCPG
                     RLWPVNLGSVERVEGLFAINERLTTRIKMADGAEMLVVKVGATNVGRIALSYKEDWIT
                     NSSKLTRQNPRLDWFATSPIAIEKGSEMARFEMGSTVILIGNKKLRDRQPDLFKSKLN
                     QAVQVGQSL"
     gene            complement(7474..8211)
                     /locus_tag="PLT31_04205"
     CDS             complement(7474..8211)
                     /locus_tag="PLT31_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94373.1"
                     /translation="MKEEPCADCGKRGEIELEVHSSFYYREWNPVAYQKWDSTAVIGV
                     LPAQVIDAIAPENCSFCHTFSADALDFDLARVEDSLFAKAYPKMPRELMFPGARLPDE
                     DSTWIAQWMARFVETPWAEGKVLKDPSPWQERLGIEQSFQRLVTDSLKNLLTQISLKY
                     NVRYLSVPILLKVEILPKAGKKGGFSWKSIWSLWDARYGELAFLTYVEFIAETKTRVA
                     PDRYWAEPFAPYLWRMLKTHPSEIENH"
     gene            8423..9520
                     /gene="ychF"
                     /locus_tag="PLT31_04210"
     CDS             8423..9520
                     /gene="ychF"
                     /locus_tag="PLT31_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HPW94374.1"
                     /translation="MGFKCGIVGLPNVGKSTIFNAITNAGAESANYPFCTIDPNVGMV
                     NVPDLRFEKLVASYKPRAIVPTVTQFVDIAGLVKGASQGEGLGNQFLTHIRECEAIME
                     VVRCFDDENIVHVHGNVDPLRDVEIIETELLLKDMDSLEKRLVTEAKNARVGTPASKL
                     RLEACEKLKNAMSEGIPARRLLGEDEVLDEVVSDLALLTAKPIFYCANVNDQDMLTGN
                     AYVDTLREYAEKQGSSVVILCGKIEEELSAMEPEDKQEFLKELGMTESGLDAVIRKGY
                     SLLGLRTYFTAGEKEVRAWTFQAGWKAPACAGVIHTDFERGFIRAETLSFADFEKHGS
                     WNAAKEAGLVRTEGKEYEVQDGDIMYFLFNV"
BASE COUNT         3224 a         1859 c         1765 g         2842 t
ORIGIN      
        1 ggaaggtgca gaagcattga gtcaaatact caaaacaaaa aatgtactac aattaaaaag
       61 taaacgagca cacgtagatg ccaccgttta tattggaaaa gactttaaca aaatcatcga
      121 acaggacaca ccatgaatga aaaaaaatct acacgaaaac caagtgtaaa aaaagcaacc
      181 actcccgtga aaacggctac tgttaaaaaa gaagcagcaa aaaaaacggt ggtaaaaaaa
      241 gaggctgcac caaaagcaac ttccaaaaaa gcaccagcta aaaaagtagc tacaacaaag
      301 acagtcacca aggctgttaa gacggtcaag gcagtgaagg ctacacctgc taaaaagaaa
      361 gaagctcttt ctgaagcagt gtctttagca gcggtttctt tgtttcaatt acgtgcagaa
      421 aacgttcaac taattgatct tcgtggtata aacgacgtcg cagactattt cttaattgcc
      481 acatgcacca gtgaagctca gatgcaagcc attctaaatg agctcaccaa agaatttaag
      541 agcaaaaaaa ttcagactaa aggaattgaa tataaaagtg gcgttcaatg ggctgtattc
      601 gatgcaggtt ttgaattgat ggttcatctt tttgaagaaa caaagagaga ggaaattcag
      661 atggatcgtc tttatagcga cgccaatatt caaaacctcc aagaaaaaga ctttattaaa
      721 aagacgacaa agaaaaaggc taacatagaa gatgatctca tttgagcaat ccattgcagc
      781 agctgttgca aagacgggta cttttttaga agaagaggtt ttacaacttc taactgttcc
      841 gcaagaaaca actcatggta attttagttt gccatgtttt gctcttgcta aaaagttgcg
      901 taaagcacct aagatcatag ctgagactct cgccacagaa atagaacttc cgcaaggaat
      961 ttctaaagtg gaacctatta atggttatct gaattttttc atagaacgtt cttttttagc
     1021 aacctctgta ttaactcaga ttgaagagaa aggtttaaat tatggccacg cttcgtcaaa
     1081 taataaaacc gttattattg attttagttc tcccaatatc ggaaaagaat tagcatttca
     1141 tcatcttcga tccaccatga taggcaatag cttgtctcgc atttacaagg cgaatggtta
     1201 tcgggtagaa cgcatcaatc atttaggtga ttggggcact gcctttggta agttaatcgt
     1261 catgtactta agagaaggtg gttctttaga agaagaagcc ctcgatcaat taacggtaaa
     1321 agaactgaac ttactatata gctctttttc aaaaattagt aaagaaaatc cagaactaga
     1381 aaatgaagct agagccgctt ttacaaagtt agaagaaggc gactctcttt atagaaagct
     1441 ctggagtgca ttcaaaaagg cgaccttaaa agaactcatg agaatttatt ccatgatggg
     1501 cgtctctttt gatcactata taggggaatc tttttttgaa gataaaatcc cagcagtcct
     1561 tgatgaatta agggagaaaa agctcctcgt gaaaagtcaa gaactcgatg tcgtaatgtt
     1621 aaatgatttt gatttacctc catgcttaat tcgtaaaagt gatggaagta ctttatatgc
     1681 gactcgagat ctcgctgctg ctctttatag aaaaaaagaa ttccaatttt cgaaatgcct
     1741 ttatgttgtc gatttaggac aatcacttca ttttaagcaa gtgttccatg tcttaaaaaa
     1801 gatggactac gaatggtatc aagatatgta ccatattcct tttggtgtta ttctacaaca
     1861 aacagaagaa gggaaatggg aaaagggaaa aacacgttca ggtaccgcca gtttacttcg
     1921 cgacgtcatt gaggccgcta aagaaaaaat ccttactttt attaacgaaa agaatcctga
     1981 actagagaat aaagaagaaa tcgcgactca aataggaatc agcgctctca cctttaatga
     2041 tctaaaaaac agaagattaa tggacgttaa atttgattgg gattcggctt taagttttga
     2101 tggagcaaca ggcccttacg ttcagaatgc tcatgtgaga ctttgtagta ttatgcgtaa
     2161 agccgcttat acagctaaaa tagaggatgt ctcttgggat actctaacag atgatgcagc
     2221 ttatgagctt attaaagtat taagccgtaa gggtaataat attgctaaag cctgtgaaac
     2281 agatgagcca agtacactcg cgcaatacgc tcttgaaata gcagaagccg ctcatcgttt
     2341 tatccatcaa gatcgagttc ttggaagtgc agaagaaaag tcaagactat atttagtaca
     2401 agccactcaa attgtacttg aaaatgtttt agatctttta ggattattcc ctattcggca
     2461 aatgtaattc tagcaattgc aattccgatt cattcgatta tattaaagac tctcttacga
     2521 gagtcttttt tatttaattt atctaaatca ttattttatt gacgaaaaca atttttcata
     2581 aaattgaatt ttgtattgca agtgatttaa tccagcacac tttatcctca aataattatt
     2641 attgtcagag accgtattgc ttttgcttta agatatgtta tatacaatat gtgcacttat
     2701 gtttatgcat ctagagcaat gccttttttt tgaatacaaa ttcaaaagca caaatcaaat
     2761 gatactttat ctataaaaaa acaatatcgc ttaacgaaaa ataatatttt atagggaaat
     2821 cattttaata cttttctata actatatttt agtggcacag gagcgtttgt atgcatctat
     2881 tgtttctcat tttattcatc gctatttctc ttcacgccgc tgtagtagaa cgttctgaga
     2941 tcagctctgt ttggtcttct cattttgttg attttgcaat cgctaccgat tctacagcaa
     3001 agctacaata cgtcagcttt tatgataaag acagaaaaat gactattgcc atgcgtagcc
     3061 tttcttcaaa tgattggcaa tttaaaacgc ttccacaaac tactggatgg gatagtcata
     3121 attatatagc catggctgta gatgatagcg gatacattca tataagcggc aacatgcata
     3181 acgcaccact tgtgtacttt cgctcttcaa agcctcattc gattgacaat tttgaatctc
     3241 tttccatgac aggctcaaac gagactaagg tcacttaccc tatttttatt aaaggcattg
     3301 aaaaaggaga actctttttc caatatcgtg ataatgcaaa taacactagt acaactattt
     3361 ggaataaata ttctacagct acaaaaacat ggactcgtct aaccacgcaa ggttttttta
     3421 ataccgaagg gctctatagt gcctacatga ctactccaat aaaaggccct gatgattatt
     3481 ttcatgtagt ctggatgtgg cgtaatactc cagtggcaaa taccaatcac gatatatccc
     3541 atattagaag taaagatctt gtccattggg aaaccatgag cggtttggat gtgactcttc
     3601 ctatcaagca atcgactcct ggagtgattg tcgattctgt ggatgctggc aacgggctaa
     3661 tcaatattgc ctttggtatt ggatgggata atcaaaatcg tgctgtgatt aattatcaca
     3721 aatatgatgt caatggaatt agccagagat ggaatacccg atgggagaac ggctcttgga
     3781 aaatctacca gacgagttcc tggaattcat tcaaatggcc tctagataga aatggaactc
     3841 ttggtattga tatttctggc agccctgtaa aaaaagatta ttcgggccga cttgttcaag
     3901 aatatagcca caaaaacttt ggtaatcgta tgtgggttct aaatgaagaa acgttacaac
     3961 cagaaaaaga tacgctacct ataattattc cagggctcga ttcattatat caagtaaaat
     4021 ctacttttcc agagatgcaa gttcatttta aagtcgatgg ggattattac cttagatggg
     4081 aaactttacc agaaaatcaa gatctagctc gtaaccctcc tcttcctgag ccaacgatgc
     4141 taagtgtcta taaaatcgat tcaaaagatc ctactcaaaa attattcagt atgaaagaag
     4201 aaaagtccta tcctactttt gtccaaaact caacgctcac gatcaatggc ttaaatgaaa
     4261 atgcatcagt ggatctttac actctcaaag ggaaaaagat ctctgcttca aagcatacaa
     4321 gctccattca agaattttca aaccttgcta gaggcaccta cctctatatt ataagaaatg
     4381 gagaagcatt gactcctgtg ataaataaag tcttcataaa ataaagtttg tattcttaga
     4441 ttgtgaaaag cttaaaaatg gcgatccaca atagtttttt tcagtaaatt cgcaaaaaaa
     4501 ttagctgcaa ttaatcgcag cttttttttg aaaagattta gcgaaacgct tcaatttaaa
     4561 tattacatca cggtgcaaac attgcttaca ggattaagga ttcttcttaa aacttgaaac
     4621 aacatctggt acaagatcaa cgatctttga aagcatcgaa ttgtctcgag aaacaggcac
     4681 tatgcgaacg tcttctccag aaatgacaag aaaagcgact ggttcaacgg aagcacctcc
     4741 gccagatcca gtgatatcac cttttccaga atggccacct aatccaaagc ctaaagaaac
     4801 tcgacttaca ggaataatcg tggtatgctc ccctgcttgt atcggttttc caactactgt
     4861 ttccgcttga gatataaagc gtaatttttc aagtaagata tctgcaaatt gttctatagc
     4921 catgtttata atataacatt tttataagca agattagtat tttgctgaat aaactcgttc
     4981 actaatttca aaaaacaagg tttctagctt ttttgaatcc aacgggcccc atacgcaaac
     5041 gactcgtccc ccacctaacc gagtcgccat agcccgagtt ccatggccac tatcaaattt
     5101 tagaatagat tctgtataag aaaccttttc tttctttttt gaaatagaat cgacccactt
     5161 aagccaatct tcgttatcaa ctgattcaaa gcttctcgct acactccaat agacatccgc
     5221 gtttcgataa cgctgcacaa acatcggaaa agagattttt tctccatgaa atagatcgat
     5281 ctgaacagag gttttccctg gctctctatt ttcaaaagga agacttagaa attcttgagg
     5341 aagcccgcca cgataccctg gaaactgttg tacatattct tctaaaaggc gcctatcatg
     5401 agggtaaaaa cgtttgtgtc taaaaataaa aagccaacgt cccgaacgaa tactctgttc
     5461 taataaagga ccttttacaa caggaagagc cccttgaaag cgcccactat taatgtaata
     5521 agcaagagca aaggcatcgt tttcaaattc aagaatagaa acccatagag agtcatctaa
     5581 atccatttta tacagtaaat gaatctcttc tagcaaatgc cattttgaag aatcttgttt
     5641 aacccatttt gctgattcat gtttatgatt ttgatagaca atcgaatcaa aagaaacaga
     5701 acgtaaagga atgccttcat cagaacacgc aataataaaa gaaagcacca tgctaatcaa
     5761 agccaatcga ttcattaaac taaggcctcc cataagagca ttcactataa agaggacatt
     5821 tttcacaaga ctttatctgt tcacgacaaa gaaaatcttt ttctcctggt tcaagaaaaa
     5881 aacttagccc atgagctgct ttatctatcg ccattggaga tagtatttta taaaaatcgt
     5941 tcacttctat tatttttttt tctggagtcc agtttgcgaa atcgtcctct tttccaggac
     6001 caataaaccc cataaaacgg cggcttccca ttgaaatagg aaaaggtaac ttagattttc
     6061 cctctcgaat agtaagacct aatcctaatg gactattact ttttaatcga tgaatcgcag
     6121 acatgacttt aggccttata ttctttttcc ccataaaata tatttcaccg agatctcgcc
     6181 atatttctgc ttcatttcgc gaagttaacc aaatgtttaa gggaaaatgg cgacggataa
     6241 aaaagccaac actccaaaac atacctgggg catgatctaa caaagcccat gattcttttt
     6301 ttaatgcttt ttgaactact gcttttatat cagaagcttt aggcacagga gtttcccata
     6361 attttttatt cggaaaagca ttaaacagag cctctaaaac tgaggccatt tgaaaaaaag
     6421 ataagttttg aaatataaca ctgccaagta aaacccaagc gatttgagag ttttcgtctt
     6481 ccgctaatga agccactttt acaactgggt ctggtacaga atcaaactct aaaataaaag
     6541 agcgtgcagc ctcaacgata tggggatttt ctctagcaaa gcgtaatccc tgtttagaca
     6601 ttataaactc tgacccactt gaaccgcttg atttaatttt gatttaaata gatctggctg
     6661 acgatctctt aattttttat tcccgattaa aatcacagta cttcccatct cgaaacgagc
     6721 catttcagaa cccttttcta tagcaatagg agaagtcgca aaccaatcta agcgaggatt
     6781 ttgacgtgtg agttttgaag aattcgtaat ccaatcttcc ttataactta aggcaattcg
     6841 ccctacgttt gtagctccca ctttaacaac tagcatttca gccccatcgg ccattttgat
     6901 tcttgtcgtt aatcgttcat taatagcgaa caaaccttca actcgttcta cacttcctag
     6961 attcactggc cacaatcgcc cagggcagta atgagcagaa acgatagaac cagaaacagg
     7021 tgtatgaatt cggtggtaat taaaaggagc taaataaata gtagcccaaa aaccaccttc
     7081 aaaatctttg gccatttctt cacttcgcaa aagagaggct aaatcatagt tttttccttt
     7141 agcttgaatc atctcttgag aattgttgtc aaaaacaccc gtctgagaaa gaacgccatc
     7201 gacagggcta acgacttctg cagaaggatc aattggacga gcaccttttt ttaaatgacg
     7261 aataaaaaga gtggcaatgt ttgggtattc agaaaaaggc ttttctgatt cttcctcatt
     7321 caactggtaa tgagaagcaa accaatcccg cgctttagca ctaatcccag ggagacgcaa
     7381 acgagtaaaa attccaaaca cttgcgaaac aaggctttta ggaagcaaaa gcataaaaat
     7441 gtaaaatgga gtattcataa gcctaaatat aaatcaatgg ttttcaattt ctgaggggtg
     7501 agtttttaac atacgccata aatagggtgc aaaaggttca gcccaataac gatctggagc
     7561 aactcgcgtt tttgtctctg caataaattc aacatatgtt aaaaaagcaa gttctccgta
     7621 acgagcatcc caaagactcc atatgctttt ccaagaaaat ccgcccttct ttccagcctt
     7681 aggtaatatt tccaccttta ataaaatagg gactgaaaga taacgtacgt tgtatttcaa
     7741 agaaatttgc gttaaaagat tttttaagga atcagtcact aatctttgaa aagactgttc
     7801 tatgcctaat cgttcctgcc atggagaagg atcttttaaa accttccctt ctgcccaagg
     7861 agtttctaca aaccgcgcca tccactgggc aatccaagtg gaatcttcat caggtaaacg
     7921 agctcctgga aacatgagtt cacgaggcat ttttggataa gcttttgcaa aaagggaatc
     7981 ttccactcta gctaagtcaa aatctaaagc atcggcacta aacgtatggc aaaaagaaca
     8041 attttctggg gcaatggcgt caatgacctg agctggaaga actccgatca cagcagtgga
     8101 atcccatttc tgataagcca caggattcca ttctctgtaa taaaacgaag aatgtacttc
     8161 tagttctatt tcgcctcgtt tgccacaatc agcacatggt tcttctttca tcgagctatt
     8221 cgatcctgta gaactacagg aaaaaattag gaagctcaca gataggatta agattatttt
     8281 tttcatcatt taaaatggta attaaatttc aatcaaaata cttctttttt tcaaaaaaga
     8341 gtcctaattc aacaaggaac cgttcttcat tttataaaaa gaactaaatt tgccacgttg
     8401 ttttttgttt tttggagaca tcatgggttt taaatgcggt attgttgggc ttccaaacgt
     8461 gggtaagagc actattttta acgccatcac caacgctggc gctgaatcag cgaattatcc
     8521 cttttgtact attgatccaa acgtaggcat ggtaaatgtg cctgatttac gttttgaaaa
     8581 attagtggca agctacaaac ctcgcgccat tgtccctaca gtgacccaat ttgtggatat
     8641 tgctggcctc gtgaaaggag ctagtcaagg cgaaggacta ggaaatcaat ttttaacgca
     8701 tattcgtgaa tgtgaagcga tcatggaagt ggtgcgttgt tttgatgatg aaaatattgt
     8761 tcacgtccac ggtaacgtag atcctttacg tgacgtagaa atcatcgaaa cagagctttt
     8821 attaaaagac atggattctt tagaaaagcg cctagtgact gaagctaaaa atgcaagagt
     8881 tggtacccct gcttcaaagc ttcgtttaga agcatgtgaa aagcttaaaa atgccatgtc
     8941 cgaagggatt cctgcaagac gtcttttagg cgaagatgaa gtcttagacg aagtcgtctc
     9001 tgacttagct cttttaactg caaagcctat tttttattgc gctaatgtga acgatcaaga
     9061 tatgctaacg ggcaacgctt atgtggatac tcttcgagag tatgctgaaa agcagggcag
     9121 ttctgttgtg attctctgtg gaaaaatcga agaagaattg tctgcaatgg aacctgaaga
     9181 caaacaagaa tttttgaaag aactagggat gactgaatct ggcctagatg ccgttattcg
     9241 aaaaggttat tctttacttg gcttaagaac ttattttact gctggagaaa aagaagtccg
     9301 agcatggact tttcaggctg gctggaaagc acctgcatgt gctggtgtta ttcatactga
     9361 ttttgaaaga ggctttattc gagcagaaac tctttcattt gccgatttcg aaaaacacgg
     9421 ttcatggaat gcagctaaag aagctggatt agttcgtacc gaaggaaaag aatacgaagt
     9481 acaagacggt gatattatgt acttcttatt taatgtttaa gagcgcctct aaaaattcat
     9541 tcatggcaag caaacacgcc atgcgttttg cacactattt taaatggtgg ttttaattca
     9601 cttcttattt ttatgtagta atggccgaat taaaaaggcg atacttattg agatgccgat
     9661 aattaagagc atacttacac agtatgctcc
//
LOCUS       DAOOBB010000060         9619 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_8829_length_9619_cov_4.268716, whole genome shotgun sequence.
ACCESSION   DAOOBB010000060 DAOOBB010000000
VERSION     DAOOBB010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 9619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9619
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8829_length_9619_cov_4.268716"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="PLT31_04215"
     CDS             <1..623
                     /locus_tag="PLT31_04215"
                     /inference="COORDINATES: protein motif:HMM:NF015387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HPW94375.1"
                     /translation="HDIDLVNEGGLYREDGVDIVGIGCDTEGANCTGGFALGYTTTDE
                     WLNYTINVEKEANYMFRANVSSGLDGGSFMLFIDNKPVTDTIKVPAGEDWNTYKFVEG
                     SIGKVTQGKHVLKVLFTGSYVNMDWILFGSTEQEMPIQDRSITSLIPSDKEFRVYNLK
                     GKLMHSMVAKGISEVREQVKESHLQGGVYMIRSTDGLIKQLIRVAK"
     gene            648..3542
                     /locus_tag="PLT31_04220"
     CDS             648..3542
                     /locus_tag="PLT31_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HPW94376.1"
                     /translation="MKKFWQSLVLIFVVSAAYAGDPYSEILWYDSDAGNTFTNAIPIG
                     NGYMGGMIYGGVEKDVINLNEGTVWSGGPGDNNKAGAADKLPSVRAALFTQDYKLAES
                     IAGQMVGYQPAAYQPVGDLNLLFSGHKGTNYRRELDLSTAISKTTYTYNGVEYTREYF
                     ANYPDKVIVIHLTANQTGKISFEASFTSPHGNKSFSTLNNNILMMNGTINSIKFQSRL
                     RISTDGGTVSAGSGKLTVSGANSATIILNIASNFKSYNDVTGDPSTLTSKVIDQVSTK
                     TYDALKSAHLKDYQALYNRVRLNLGTPASSAGDITTNRVKFFNSNDDPSFVELYYQFG
                     RYLMISSSRAGGQPANLQGIWNRDLSPTWGSKYTTNINLEMNYWMVESANLQECIMPL
                     VDKIKAMVAQGSKTAKVHWGTDKGWVVHHNTDLWNRTAPIDGSWGVWPTGAGWLSTHL
                     WEHYLFTQNKDFLKDVYPTLKSAAEFLLAILVEEPVSGKKYLVTAPSASPENTHGGYN
                     VCFAPTMDNQIIRDAFNYVIEASKVLGVDNELRIQLEAAVKRLPPDQIGKHSQLQEWF
                     FDWDDPRSDHRHVSHLYGLFPSAQISVDETPNFAEAAKQTLIQRGDLATGWSLAWKIN
                     LWARLQDGDHAYNLVRLLLTPDRTYNNLFDAHPPFQIDGNFGAVSGINEMLLQSQGGK
                     IRILPALPSKWATGNIAGIRARGGVVVDSLAWKDGKLTYLKMTSENNTPLHLEYAGKT
                     FSITTKSGSSYELDGNLKLLNQPFEAVVIPARIQAEDYVEMSGVEIETGSDNEVNIGW
                     INDGDWVSFLVRSPSAATFKWRISVGSAAEEASEIILKDSSGNVLARQTIDLTKTTGW
                     NDWYETETDVELPAGVQKLTMEFTGTSTYLVNVNWIEFENTTLFGSALKMDAQQQNRP
                     CQIFDMNGKYLGQLIIQDFSQAVSSMQAKNYRRGIYLLMDKTNAKVIHLQ"
     gene            3566..5554
                     /locus_tag="PLT31_04225"
     CDS             3566..5554
                     /locus_tag="PLT31_04225"
                     /inference="COORDINATES: protein motif:HMM:NF027814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HPW94377.1"
                     /translation="MNQIKNIILFTAVAILPAFSQINDLVKTPPMGWNSWNVFHENIN
                     ENQIKEIADVMVSSGMKDAGYIYLNLDDNWMAKTRNANGDLQADPTRFPSGMKALGDY
                     IHEKGLKFGIYGDRGLRTCHHYNNSSQFPNSGSGSYMKEERDAKTFASWGVDYLKYDN
                     CEPAPGSNQRQDYERMRDALLKSGRDIVFSICAWGYQDWMPKTGNLWRTTGDISNAWE
                     AKPGDWFRGVADIIEENEKLYIHAGPGKWNDPDMLQVGNGKLTMDESRSHMTMWAMMA
                     APLLTGNDLRKMTPEIKDIYMNAEMIAVNQDSAGIAGHRVSNENGKQIWIRYLGSQSS
                     GNIAVALYNKNNTTQTIDLNFADIGITGEVSVRDIWQKKDLGLLKTSLSAEVPAHGTT
                     FLRLTTKLDPAEPFGDNAIAIPGKLETENYDKGSSGLAYSDNDIANQGNAYREGGVDV
                     VGLNCAAVAEGTFLKSSDCEGFAIGYTNVGEWMRYTVNIAKTQTYYVVARVSSGSENS
                     SFRLFLNDKAITDTIKVPQGADWDTYTTVSVTSQEMTAGEQALKLQITGSYVNVDWLY
                     FCEEENCADTIKTAIHAGVNNQNLELMPQVKVSEFNVFSITGKHLGRIHFDGSMLSNS
                     ISKNDAFAKEMKFAGYTNGVYIVRAVKGDKALKIVVKE"
     gene            5610..7373
                     /locus_tag="PLT31_04230"
     CDS             5610..7373
                     /locus_tag="PLT31_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HPW94378.1"
                     /translation="MDISKVLIIIAMGCLPTFAVTNQFRGVNWADKRDNFVSEILVLS
                     GLSLSDTYESASIVADRVIGQFVELLGTNSVRLPINEPTISSFWNTYTGVIDVGLKKG
                     RVVLGYWGPAQPSGPKNMNDWWAMWDTVVNKYGEDPNAYFEIFNEPHMYTKEELRNLY
                     ASWLTRYPNVPRDHIILDGSGMAQNVPDIASDSRFDGCLFAVHEYTFWNMSITTEAGW
                     KNSIKGKVGNYSDRTICTEWGGAMSPGDKAGVHYETMDYNQQPTNYFMAYIRGIPEQL
                     REWKMGSFYWPGLRDGDWYSMTKRSGEGANIKLEIVNQSGVDRMQYSWTDTVEVPSVP
                     QEPFGSFNGELEIAGEPTSIPGTIEVENFDLGGNQVSFYDKDTENKGNAYRKNAVDVV
                     GLDCAEIAEGVFVKGTDCAHYALGYTEAGEWLEYTVNVESENVYTIEARVASGGDNSS
                     FRLFLDDEPIADTIKAENTGDWDTYKTISAKTIKLSKGEHILKLLITGSYVNIDNLKF
                     TEGTIRLSEPLHFISRKGMQEYRVYHLNGALLGNYNAVDMNSLKREMHRSNLKSGIYF
                     VRSKTGQINQLMEIKKQGVRI"
     gene            7370..9322
                     /locus_tag="PLT31_04235"
     CDS             7370..9322
                     /locus_tag="PLT31_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HPW94379.1"
                     /translation="MKKNLKVFFMLTSIFFFSIQLNAAEFVLKVSLSDSLRPATHCAS
                     GSLYGMTETLPSDINAHVSPLKPNVFLQPAISGSGHQQPIGDAFKVAERLQGTTGKVQ
                     IRLADILPGWPYQWPGKNKWLAGVKSIIEKKLASGLTNFDGYEIWNEPDGTWKSENGD
                     FHSTVWKPTYDLIKSMDPTAKIIGPSYSYYNSGRMSDFLKYTKANNCLPDVISWHQWG
                     SGGFIGALENYKSLEKSNGISERKLSINEYSSDTHAYEGSPGISVPFIAKFERNGVES
                     AMISWWFTNLPGRLGSLLTANNEKGGGWWLYKWYGDMTGYMANVVPPNDKSEGVDAFA
                     AVDEKQRYASIILGGNSIGTVNMNIEGIPSFFGSEVEVLIEYVTWENKDKAVSSVQKI
                     SSNKYSINDQKLSIPVNVTSQFYAYRLYITPTEQLPQEPYKGVISIPGTVEVENYDLG
                     GNNNAYYDMEYENKGGEYREDGVDVVTNDNGYAIGYTMDTEWLEYTVNVESENVYTIE
                     ARVASGGDNSSFRLFLDDEPIADTIKAENTGDWDTYKTISAKTIKLSKGEHILKLLIT
                     GSYVNIDNLKFTEGTIRLSEPLHFNSQNEIQEYRVYHLNGALLGTYNAMDIASLKSEM
                     HSSNLKTGIYLVRSKTAKINQLIELK"
BASE COUNT         3090 a         1591 c         2136 g         2802 t
ORIGIN      
        1 atcatgatat tgatttagtg aatgaagggg ggctctatcg tgaagatggc gtcgatatcg
       61 tgggcatagg ttgtgatact gaaggagcca attgtacggg tggctttgct cttggctata
      121 cgactacaga tgaatggctg aattacacga ttaacgtaga aaaagaagcc aattatatgt
      181 tccgtgcgaa cgtttcaagt ggacttgatg gaggctcatt catgttattc atagataata
      241 agcctgtcac cgatacgatt aaagtaccag ctggcgaaga ctggaataca tataaattcg
      301 tagaaggttc tattggaaaa gtcactcaag gaaaacatgt actgaaagtc ttatttacag
      361 gtagttatgt gaatatggat tggattcttt ttggatcgac tgaacaagaa atgccaatac
      421 aggatcgctc aatcacttcc ttaatcccga gcgacaaaga atttcgagtg tataacttga
      481 aaggaaagct catgcattcc atggtggcaa aaggaatctc tgaggttcgt gaacaagtga
      541 aagaatcgca tttgcagggt ggagtttata tgatacgatc aacagatggt ttgataaaac
      601 agttgatacg agtcgctaaa tagtagggct atcaaaaagg tcgatttatg aaaaaatttt
      661 ggcaaagcct tgttctcatt tttgtggtct cagcggccta tgctggagat ccatattctg
      721 agatcctttg gtatgatagt gatgctggta atacatttac gaatgctatt ccaattggta
      781 acggatacat gggcggcatg atttatggtg gcgttgaaaa agacgtgatt aacctaaatg
      841 aaggcaccgt gtggtcggga ggccctggag acaacaataa ggcgggagct gctgacaaac
      901 tgccctctgt tcgtgccgct ttgtttactc aagattataa actagccgaa tcgattgctg
      961 gtcaaatggt tggctatcaa cccgccgctt atcagcctgt aggtgatctc aacttattat
     1021 tttcaggtca caaggggaca aattatcgcc gagaattaga tttaagtacg gccatttcta
     1081 aaacgactta tacttacaat ggagtcgaat atacaagaga atactttgcg aattacccag
     1141 ataaggtaat agtgattcac ttgacagcaa atcaaacagg aaagatttct tttgaagcct
     1201 cttttacatc gcctcatggg aacaagtctt ttagcacttt gaacaataat attctcatga
     1261 tgaatggaac gattaattcc ataaagttcc agagccgtct tcgtatttcc acagacggag
     1321 gcacggtatc agcaggtagt ggtaagttga ctgtttctgg agctaattct gctactatta
     1381 ttttgaatat tgcaagtaat tttaaatcgt ataatgatgt tacaggagat ccatctactt
     1441 taacgagtaa ggtgattgat caagtatcta caaaaactta tgatgcctta aaatcagcac
     1501 atttaaaaga ttaccaagct ttgtacaatc gcgtgcgttt gaatcttggt acacctgcaa
     1561 gtagtgctgg tgatattacc accaatcgag tcaaattttt taattctaat gatgatcctt
     1621 cttttgtaga actttattat caatttggtc gatacctgat gatttcaagt tctcgtgcag
     1681 gcgggcaacc agctaatttg cagggaatat ggaaccgaga tctttctcct acttggggaa
     1741 gtaaatacac gactaatatt aatttggaga tgaattactg gatggtggaa tcggcaaatt
     1801 tgcaagaatg tattatgcca cttgtggata aaattaaggc gatggttgcg caaggatcaa
     1861 aaacagcaaa ggtacactgg ggaacagata aaggttgggt tgtgcatcat aataccgatc
     1921 tctggaatcg tacggcaccg attgatggtt cttggggtgt atggcctaca ggtgctggct
     1981 ggctgagtac tcatttgtgg gagcactatc tttttactca aaataaagat tttttgaaag
     2041 atgtgtatcc aactttgaaa agtgcggctg aatttttgtt agcgattctt gtagaagaac
     2101 ctgtgagtgg caaaaaatat cttgtgacag ctccgagtgc ttctccagaa aatacccatg
     2161 gtggatataa cgtttgtttt gctcctacta tggacaacca gattattcga gacgccttta
     2221 attacgttat agaagcttca aaagtattag gcgtagataa tgagttgaga attcaattag
     2281 aagccgctgt taaaagatta cctccagatc aaattggaaa acacagtcaa ttacaagaat
     2341 ggttttttga ttgggatgat ccaagaagcg atcatcgtca tgtttctcat ttgtatggtc
     2401 ttttcccaag cgctcaaatc tctgtggatg aaacaccaaa ttttgcagag gctgcaaagc
     2461 aaacattaat tcaaagaggt gatttagcga ctggatggtc tttggcatgg aaaatcaatt
     2521 tatgggcacg tttacaagat ggagaccatg cttataattt agttcgcttg cttcttactc
     2581 ctgatcgtac ctacaataat ttatttgatg cacatccacc atttcaaatt gatggaaact
     2641 ttggtgctgt gtctggaatt aatgaaatgc tattgcaaag ccaaggcgga aaaattcgta
     2701 tccttcctgc gttgccttca aaatgggcta caggaaacat tgctggtata cgtgcccgtg
     2761 gtggcgtggt cgttgattct cttgcatgga aagatggaaa acttacttat ttgaagatga
     2821 cttcagaaaa taatactcct ctccatttag aatatgcggg taagactttt tctattacta
     2881 caaaaagcgg ttcctcttat gaattagatg gaaatttgaa attattaaat cagccttttg
     2941 aagccgttgt aattcctgca agaatccaag cagaagatta tgttgaaatg tcaggagtag
     3001 aaatagaaac aggctctgat aatgaagtga atatcgggtg gatcaatgat ggcgattggg
     3061 tgtcttttct tgtgcgttct ccttcggcag ctacattcaa atggcgtatt agcgtgggtt
     3121 ctgctgcaga agaagcaagt gaaattatat taaaagattc ttcgggaaat gttcttgcaa
     3181 ggcaaaccat tgatcttaca aaaacgactg gatggaatga ttggtacgaa acagaaacgg
     3241 atgtggagct tcctgctggc gtacaaaaac tgactatgga atttacaggc actagtacct
     3301 atttagtgaa cgtgaattgg attgaatttg aaaacaccac tttgtttggg tctgctctaa
     3361 aaatggatgc acaacaacaa aaccgtccat gtcaaatttt tgatatgaat ggaaagtatt
     3421 tgggacaatt gataattcaa gactttagtc aagcggtttc atcaatgcag gcaaaaaatt
     3481 atagacgtgg tatttatttg ttaatggata aaacaaatgc aaaggtgatt cacctacaat
     3541 aagtttaaaa tgcaagtata ataatatgaa tcagataaag aacatcattc tttttacagc
     3601 ggtagcaata ctacctgctt tttcacaaat taatgattta gttaaaacac ctccgatggg
     3661 atggaatagc tggaatgttt tccatgaaaa cattaatgaa aatcaaatta aagaaattgc
     3721 cgatgtcatg gtcagttcag gaatgaaaga tgctgggtac atatatttga acttggatga
     3781 taactggatg gcaaaaacca gaaatgccaa tggcgatttg caagcagacc ctactcgttt
     3841 tccaagtgga atgaaggcat taggtgatta tattcatgaa aagggcttga agtttggtat
     3901 ttatggtgac cgtggattac gcacttgcca tcattacaac aacagcagcc aattcccgaa
     3961 ttcggggagc ggaagctata tgaaagaaga acgagatgca aagacttttg cttcttgggg
     4021 tgtagattac ctaaagtatg ataactgcga accagcacca gggtcaaatc aaagacaaga
     4081 ttatgaacgc atgcgagatg cgcttttaaa atctggtcga gacattgtat ttagtatttg
     4141 tgcatggggt tatcaagatt ggatgccaaa aactggtaat ctatggcgta ctacaggaga
     4201 tattagcaat gcatgggaag caaaaccagg ggattggttc cgcggtgtgg ctgatattat
     4261 tgaagaaaat gaaaagcttt atatccatgc tggtcctgga aaatggaatg acccagatat
     4321 gttacaagtg ggtaatggaa aattgacaat ggatgaatca agatctcaca tgacgatgtg
     4381 ggctatgatg gctgctcctt tgctcacagg aaatgacttg cgtaaaatga ctcctgaaat
     4441 taaagacatt tatatgaatg cggaaatgat tgccgtgaat caagactctg caggcattgc
     4501 agggcatcgt gtgagtaatg aaaatgggaa acaaatttgg attagatatc ttggttctca
     4561 aagcagtggt aatatagccg ttgctttata taataaaaat aacacgactc aaaccataga
     4621 tttgaatttt gcagatattg gtatcacagg agaagtgagc gttcgtgata tttggcagaa
     4681 aaaagatctc ggtcttctca aaacaagtct ctctgcagaa gttcctgcac acggcacaac
     4741 tttccttcgc ttaacaacaa aattagatcc agcagaacct tttggagata atgcgattgc
     4801 cattccaggg aaattagaaa cggaaaatta cgataagggt agctctggcc ttgcttatag
     4861 cgataacgat attgcaaatc aagggaatgc atatcgtgag ggcggagtag atgtcgttgg
     4921 cttaaattgt gctgccgttg cagaaggtac attcttgaag agttcagatt gtgaaggatt
     4981 tgctatcggt tatacaaatg tgggcgaatg gatgcgctac acagtaaaca tcgcaaaaac
     5041 tcaaacgtac tatgtagtgg ctcgcgtttc tagcggttca gaaaattcta gtttccgtct
     5101 attcctaaat gataaagcca tcactgatac aatcaaagtg cctcaaggtg ccgattggga
     5161 tacatacacc acggtgagtg tgactagtca agaaatgaca gctggtgaac aagctctcaa
     5221 attacagatt acaggcagtt acgtgaatgt ggattggctt tacttctgtg aagaagaaaa
     5281 ctgcgcagat actatcaaaa ccgcaattca tgctggagta aataatcaga atcttgaatt
     5341 gatgcctcaa gtaaaagtaa gtgaattcaa tgttttcagc attacaggga agcatcttgg
     5401 tcgtatccat tttgatggct cgatgctctc gaatagtatt tcaaagaacg atgcttttgc
     5461 aaaggaaatg aagtttgctg gttatacaaa tggcgtatat atagtgcgtg ctgtaaaagg
     5521 agataaagca ctaaaaatag tagtgaaaga ataaacctag tattttggaa tacgctttgt
     5581 ggagattggg tattccatag ggaaacatta tggatatttc taaagtctta ataattattg
     5641 cgatgggttg cttgccaact tttgccgtca caaatcagtt tcgtggtgta aactgggctg
     5701 ataaacgaga taatttcgtc tcggaaattc ttgtgctttc gggattatcg ctttcggata
     5761 cttatgaatc tgcttcgatt gtggctgatc gcgttatagg tcagtttgtg gaattgcttg
     5821 gaactaatag cgttcgactt cctattaatg aacctaccat ttcctcgttc tggaatactt
     5881 atacaggcgt tattgatgtc ggcttaaaaa aaggacgtgt cgttttaggc tattggggcc
     5941 ctgcacaacc atctggccca aaaaatatga atgattggtg ggctatgtgg gataccgttg
     6001 taaataaata cggcgaagat ccaaacgctt attttgagat attcaatgag cctcatatgt
     6061 acacgaagga agaactcagg aatctttatg cctcatggct tacaagatac cctaatgttc
     6121 ctcgtgatca catcattctt gatggaagtg gcatggcaca gaatgtgcct gatatagcaa
     6181 gtgatagtcg ttttgatggc tgcctttttg cagtacatga atacacgttt tggaatatga
     6241 gtatcacgac tgaagctggc tggaaaaatt ccattaaggg aaaagtgggc aactattctg
     6301 atcgtaccat ttgtacagaa tggggtggcg ctatgagccc tggtgacaaa gcgggtgtcc
     6361 actacgaaac aatggattac aatcaacaac caaccaacta ctttatggcc tatattcgtg
     6421 gcattccaga acaattgcgt gaatggaaaa tgggtagctt ctattggcct gggcttagag
     6481 atggcgattg gtatagcatg acaaaacgca gtggtgaagg ggcgaatatc aagctagaga
     6541 ttgtaaacca atctggagtc gatcgcatgc aatattcttg gacggataca gttgaagttc
     6601 cgtctgtacc acaagaaccg ttcggaagtt ttaatggcga actggaaata gctggcgaac
     6661 ctacttctat tccagggact attgaagtag aaaattttga tttgggtggt aatcaagttt
     6721 cgttttatga taaagacaca gaaaataaag ggaatgctta tcgaaaaaat gctgtagacg
     6781 ttgttggtct tgattgtgct gaaattgccg aaggtgtttt tgtgaaagga accgattgtg
     6841 ctcactatgc tctaggctat acggaagctg gtgaatggct tgaatacacc gtaaacgtgg
     6901 aatctgaaaa cgtttacact atagaagcac gtgtggcttc tggaggagat aattcttcgt
     6961 ttagattatt cttagatgat gaaccgatag cggatactat taaagcagag aatactggcg
     7021 attgggatac ttacaagacc atttcagcta agacgattaa actctccaaa ggggaacaca
     7081 tattaaaact tctcatcact gggagctacg tcaatattga caatttgaaa tttacagaag
     7141 ggacgattcg cctttcagaa ccgcttcact ttatttctag aaaagggatg caagagtatc
     7201 gcgtctatca tttgaatggg gcgcttcttg gtaattataa tgcggtagat atgaattcac
     7261 tcaaaagaga aatgcatcgt tccaatttaa aatcaggtat ttattttgtg cgttcaaaaa
     7321 caggacaaat aaatcaactc atggagataa aaaaacaggg tgtaagaata tgaagaaaaa
     7381 tttaaaagta ttttttatgc taacaagtat attctttttt agcattcaat taaatgcagc
     7441 agagtttgtt ctaaaagtct cgctatctga tagtttaagg ccagcaacgc attgtgcttc
     7501 tggttcatta tatggaatga cagaaacatt accttcagat ataaacgcac atgtttctcc
     7561 attaaaacca aatgtttttc ttcagcctgc aattagtgga agcggtcatc agcaacctat
     7621 aggagacgct tttaaggtgg ctgagcgatt acagggaacg acaggaaaag tgcagattcg
     7681 tttagcagat attttgcctg gctggccata ccaatggcca ggaaaaaata aatggcttgc
     7741 tggagtgaaa agtattattg aaaaaaaatt agcctctggt ttaaccaatt ttgatggcta
     7801 tgaaatttgg aatgaacccg atggcacatg gaaaagtgaa aacggtgatt ttcacagtac
     7861 agtatggaaa ccgacttatg atttaatcaa atcgatggat ccaacagcaa aaattattgg
     7921 cccttcttat tcgtattaca atagcggcag aatgtctgat ttcttaaaat acacaaaggc
     7981 taataattgc cttccagatg taattagctg gcatcaatgg ggttcaggag gctttattgg
     8041 agctctagaa aattataaat ctttagaaaa atcaaatggc atttcagaac gaaagttaag
     8101 tattaatgaa tactcttcag atacacatgc ttatgaaggg tctccaggaa tttcagttcc
     8161 tttcattgcc aagtttgaaa gaaatggcgt tgaaagtgcg atgatctcct ggtggtttac
     8221 gaatttacca ggtcgtttag gcagtctatt aactgcaaat aatgaaaaag gtggaggttg
     8281 gtggctttat aaatggtatg gtgatatgac tggttatatg gctaatgtgg ttcctccaaa
     8341 tgataaaagc gaaggtgtcg atgcatttgc tgcagtcgat gaaaaacagc gctacgcgag
     8401 tattattctt ggtggaaaca gcattggtac cgtgaatatg aatatagaag gtattccttc
     8461 tttttttggc tcagaagttg aagtattgat tgagtatgtc acttgggaaa ataaagataa
     8521 agcggtaagt agcgttcaaa aaatttcatc caataaatat tcaattaatg atcaaaaact
     8581 ttccattcct gtcaatgtca caagtcagtt ttatgcgtat cgtctttata tcacacctac
     8641 agaacaactt cctcaagaac cctataaagg tgttatctca atcccaggaa ctgtagaagt
     8701 agaaaattat gatttgggcg gtaataataa cgcctattat gatatggagt atgaaaataa
     8761 gggcggagaa tatcgtgaag atggcgttga tgtagtgaca aatgataatg gctatgcgat
     8821 agggtatacc atggataccg aatggcttga atacaccgta aacgtggaat ctgaaaacgt
     8881 ttacactata gaagcacgtg tggcttctgg aggagataat tcttcgttta gattattctt
     8941 agatgatgaa ccgatagcgg atactattaa agcagagaat actggcgatt gggatactta
     9001 caagaccatt tcagctaaga cgattaaact ctctaaaggg gaacacatat taaaacttct
     9061 catcacaggg agctacgtca atattgacaa tttgaaattt acagaaggga ctattcgcct
     9121 ttcagagccg cttcatttca actctcaaaa tgagatacaa gagtatcgcg tctatcattt
     9181 gaatggagca cttctgggta cttataatgc catggatatt gcttcactta aaagtgaaat
     9241 gcatagttcc aatttaaaaa caggtattta tttagtgcgt tcaaaaacgg caaaaataaa
     9301 tcaactcata gagttgaagt aagaattttt gcatccaaaa atgcaaaaaa aaagccacag
     9361 atacataaaa gtggctcctt tacgagacca aaagaggcat gagatctttt agatttcatg
     9421 ccttttttat aatgaaattt tataaatcaa atattcacta aaaaaaatga taaatcacta
     9481 ttaaagggcc ttagtaaggc atttaaaaat aaaaggaaag ttttattctc tcgtaaatga
     9541 tatattaacg gatatacaag aaaacaatgg agttgaaatg acaaaaagaa caatttcgga
     9601 agtggtacga ggccaacct
//
LOCUS       DAOOBB010000061         9585 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_8881_length_9585_cov_3.691920, whole genome shotgun sequence.
ACCESSION   DAOOBB010000061 DAOOBB010000000
VERSION     DAOOBB010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 9585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9585
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8881_length_9585_cov_3.691920"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /gene="radC"
                     /locus_tag="PLT31_04240"
     CDS             <1..618
                     /gene="radC"
                     /locus_tag="PLT31_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HPW94380.1"
                     /translation="EQGTSVLSTKELLALILGKGSKNKNVFSLAEELAEYISVQPRVP
                     KLEDLLKIKGLGPVKASQILACLELSGRFLFSGIRQEINSPEQALNSLSFLKNALQEN
                     FVVISLNSANNIIETHTLTVGLANQTQVHPREAFTKAIENRAVAVIFAHNHPSGNLDP
                     SSDDLNITRTLCKAGQLLSIPVLDHLIVSPSGFLSLKREHPSLFE"
     gene            747..3596
                     /locus_tag="PLT31_04245"
     CDS             747..3596
                     /locus_tag="PLT31_04245"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPW94381.1"
                     /translation="MKRLYLSLLLFAITSVYASDTKTINKYGFAGVNKTQSAQSLGHS
                     KLTFSILGNVTNDFSMIEDRGGDSQHLTQFLSYKNGDPSDTSYSKINDFLGINSYISF
                     GLGLSNYFDIGVTLPVYYERVGTETNGNSPVTGTDVGYIGNLKADIKIRLPLPDYQPF
                     DIALFGGIDIGTSDLGEDGMWVREPEYINTESLKAESFGYDNLNLLFRGGGALSIDFG
                     KLDGGIPWIFHFNGGYRYVKPQDADSTSYLNIIHGSVATEFFFFEFLSLFAEYYMDFM
                     PEFKNSSNGKDETLNLAELTGALVFHLPVGLDVHIGGSYSFVNDQYINNLTAYHIDDD
                     NHTRTITYNGRIVPEYSVYGGITWSGFLLPQDRDDDTVVDEEDSCPDEFGHIRNGGCP
                     FPEPDIDGDGFCDAWVSEKGLLDIFVDVCKGVDECPNEKGEGKDGCPLDNPDTDADGI
                     CDPWVKQKKMSKRFKGVCKGVDSCPTQAGPTTNKGCPQDNPDVDSDGICDPWVSQKDK
                     LSDFANLCKGYDNCPGEAGPAANKGCPWPDPDTDGDGLCDEWVIKQKMGYFFENPTDS
                     TVKKCKSVDKCPYEFGPADNEGCPFDNPDTDNDSVCDQWVALKKQQDRFVNVCTGSDK
                     CPTEAGLLINNGCPVEDPDIDKDSLCAPWVKEKGMQDQFKTICKGIDNCPTEFGPKFN
                     GGCPIDDPDPDKDSVCASWVIEKGMLDQFKDVCKGSDKCPTEFGPVFNNGCPVADPDP
                     DKDSVCASWVTEKGLLKEFESVCKGADRCPTEVGPIENKGCPLEDPDLDKDGVCDPWV
                     TQKKLLSKFVNTCKGLDRCPLDSGSVESNGCPVKEIEENVKLEGVTFKSGSSILESNA
                     KKVLKVIAEQLLAPENASVNIEIHGHTDNVGRPAKNKKLSLQRAQSVVNYLASQGVPK
                     KRMKAFGHGDEEPVADNKTKEGREQNRRIEMHRAE"
     gene            3762..6002
                     /locus_tag="PLT31_04250"
     CDS             3762..6002
                     /locus_tag="PLT31_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPW94382.1"
                     /translation="METLYLIALILIFTLAIFDLFVGVSNDAVNFLNSAVGSKAFKFK
                     TILFIAALGIFVGASFSSGMMDIARHGIFQPQHFYFVEIMAIFMGVMITDVILLDTFN
                     FFGMPTSTTVSMVFELLGGAFALSLFKIIQSNGDLSFGMLINTEKALSVIFAIFVSVA
                     IAFTFGTIVQYITRLMLTFDFQKRLKYFIGLFGGLSFSSIVYFILFKGLKDSVIAKLP
                     FFHFIKDYSIELFIGAFIFFALFSYLLLFLKINVFKWIVALGTFSLALAFAGNDLVNF
                     IGVPLAALSSFQDFSANGTSVDTFLMTSLQSSASGDSWILVVAGVIMVIALLTSKKAH
                     NVIKTSLSLSSQGESDEPFGTNPVARAVVRWSHSIGESIAHMLPKRLLKWTHSRLDDS
                     KLTLHAEGASFDLVRASVNLMLASLLIAAGTSLKLPLSTTYVTFMVAMGTSLADKAWG
                     RDSAVYRISGVLSVIGGWFFTAFAAFISAFIVCALIYIGGFPILFSFFVVVIFLLVRS
                     SKRFKKKAHAKDLAQERFHKIMISNDPKETLELVRDHYKEEWGWHLIWVEDRYRDLIT
                     AFITEDLTKLRMVNHKIDDQKNVVKRLKRQGTLASRKMSSDDAIAKTFFLIQANDYAS
                     DMVFHLDRICDPSLQHVDNHFAPISKNQKKVLDSLWSEITDYIEDCGRMIFSGIYDNC
                     MDLEERGRILSEKVISIRKEQTDLIKNQKSNTRASIVFLTILYETKSIVDTSLFLTKF
                     SRKLMS"
     gene            6002..6256
                     /locus_tag="PLT31_04255"
     CDS             6002..6256
                     /locus_tag="PLT31_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94383.1"
                     /translation="MTILDQILTEANLDNHALVEASSKQLSHKTVQKARTGKRPLSKK
                     MKVLVTEALNKTLQPEKLYKKEYLFPNNLSEELEENNESN"
     gene            complement(6412..6771)
                     /locus_tag="PLT31_04260"
     CDS             complement(6412..6771)
                     /locus_tag="PLT31_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94384.1"
                     /translation="MKKQILFFTSILFVLFLAGCSEKEKPNYQGFWLGEENMIFEVKQ
                     TSDGKYTVSNINGSLNAEYKDEALRGKNSLDMEFYMTVKGDSAYYTFGTITTGYKRID
                     EKSYKDIFNSLKPMTAQ"
     gene            6815..7327
                     /locus_tag="PLT31_04265"
     CDS             6815..7327
                     /locus_tag="PLT31_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94385.1"
                     /translation="MRYLYLLPLFFVIIIAIFVWQNTTKSFSKDSIIESSSSIFISSD
                     SFKTYSFSADDVHRLLLARTKQKKGVYLDKMEPSLDSLALDIVCVFHEVMGDSYVPTI
                     TSANDYPYHARFSKHYYNKAIDFRTKDLNSRQKKELLEKAQIRIYKKGRVLLEVNGKS
                     NEHLHLELLN"
     gene            complement(7324..8526)
                     /locus_tag="PLT31_04270"
     CDS             complement(7324..8526)
                     /locus_tag="PLT31_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014544894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPW94386.1"
                     /translation="MTSPKEKNEAKKVKRSKRRKTVILVLMLLLLITLFLVQCELQKI
                     KENELNKQKAQTELLLWQKQRADSLKKLDSLKVLENSLNDSLRVADSIRVADSLRKAD
                     SLSQTKVPIIKKQDSISPKINQDSIKAIEIAEALKRDSLEKIRIQDSIRKLDTIPPEA
                     FLTPPPGRYYEPIRLKVKCDEIKCKTWISVNDTTQKQDASTGVEYNKTGDVFFKAEDS
                     LGNSTPWQKGSFDMASDNRCGKNGFSVPVNGKEICVDAYEYPNVADEIPRDMVSHEQA
                     MALCEKEGKRLCSIDEWQAACRGKDKFTYSYGNKYHPFKCNTNTKKVMRSGRKDQCRS
                     WWGMNDMNGNLWEWTSTPSSQKANSFLVAGGAWNGQNLTQCKENKFSFYPQNQYPYVG
                     FRCCREAP"
     gene            complement(8544..9362)
                     /gene="cdaA"
                     /locus_tag="PLT31_04275"
     CDS             complement(8544..9362)
                     /gene="cdaA"
                     /locus_tag="PLT31_04275"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014544895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HPW94387.1"
                     /translation="MILFKLFGVIDVRMADILDILLISIILYYVFLLFRGTRAVQMFF
                     GGALLLLAWFLAQWWELHTLVWMLSNVATLGVVAVVILFQPEIRGALTRIGQGVSQTN
                     WRSIFFHSSGLNETTEALVSAVQDLAKNRFGALIVLEKRVGLRSYADTGEQLNAIISC
                     RLLRSIFFPNSALHDGAVIMNHKNIVAAGCILPLPSTSSDQESSLGMRHRAAKALAAE
                     SDAMVIVVSEETGIISIAYRSTLRRKLSIKELEEEIKRHWRILFEEVKAEKNDD"
     gene            complement(9349..>9585)
                     /locus_tag="PLT31_04280"
     CDS             complement(9349..>9585)
                     /locus_tag="PLT31_04280"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPW94388.1"
                     /translation="ISSAADFSNLDFPILFGLSRKSYIGKIHALETSDRFIPSVVSAF
                     ITALGGATIVRVHDVASTFESFKLLEAFRSYDSI"
BASE COUNT         2931 a         1600 c         1784 g         3270 t
ORIGIN      
        1 gagcaaggaa catcagtgct ttccactaaa gaattacttg ctttaatatt agggaagggg
       61 agtaaaaata aaaatgtatt ctctttagct gaggaattgg ctgaatatat ttcagtacag
      121 ccaagagtgc caaaactaga ggacttattg aaaataaaag ggcttggtcc tgttaaagct
      181 tcgcagattc ttgcctgttt agaattgtcg gggcgttttt tgttttctgg aattcgtcaa
      241 gaaattaact ctccagagca agccttaaat tcactttcgt ttctaaaaaa tgcacttcag
      301 gaaaattttg tcgttatttc tttaaattct gcaaataata taattgaaac acacacattg
      361 actgttggac ttgctaatca aactcaagta catcctagag aagcatttac aaaagccatt
      421 gaaaatagag ctgtcgccgt tatttttgca cataatcacc cctctggcaa tttagatcca
      481 agctctgatg atctaaatat tactcgaacg ctgtgtaagg ctgggcaact actgtctatt
      541 cctgttttag atcacttaat agtgagtccc tcaggctttc tcagcttaaa aagggaacat
      601 ccttctctat ttgaataaaa atagatgata ttttcgtcat aaaaatagct tttattttcc
      661 aaaatctttg aaaaattaga ttttggaact agattaaggg aaataagatt tatttctgtt
      721 tagctatctg tatcaaagga gattgtatga aacgacttta cttgtcctta ttgctttttg
      781 caataacaag tgtttacgct tcggacacaa aaacgattaa taaatatggt tttgctggtg
      841 taaataaaac tcaatcagca caaagtcttg gacattctaa actaactttt tcaattttag
      901 gaaacgtgac gaacgatttc tcaatgattg aagacagagg tggcgactct caacacttaa
      961 cacaattttt atcatataaa aatggagatc catctgatac gagctatagc aaaatcaacg
     1021 atttccttgg aattaatagt tacatcagtt ttggactagg cttatccaat tattttgata
     1081 taggggttac tctccctgtt tattatgaac gagtcggtac tgaaacaaac ggtaattctc
     1141 cagttactgg aaccgatgtc ggctatattg gaaatttaaa agctgatatt aaaataagac
     1201 ttcctcttcc tgattatcaa ccatttgata ttgctctttt tggtggtatc gatatcggta
     1261 catcggattt aggtgaagat ggtatgtggg ttcgtgaacc agaatacatt aatacagaat
     1321 cattaaaagc tgaatctttt ggttatgata accttaatct tctatttcga ggtggaggag
     1381 ctttatcgat tgactttggt aagctagatg gaggcattcc ttggattttt cattttaatg
     1441 gcggttaccg ttatgtaaag cctcaagatg ctgactcaac ttcttatcta aatattattc
     1501 atggaagtgt ggctactgaa tttttcttct ttgaattcct ttctttattt gctgaatact
     1561 acatggattt catgcctgaa tttaagaact cctcaaatgg taaagatgaa actttgaact
     1621 tagctgaatt aacaggagct cttgtttttc atcttcctgt aggtcttgat gttcatattg
     1681 gtggatctta ttcctttgtt aatgatcagt acattaacaa cttaactgct tatcatattg
     1741 atgacgataa tcatactaga acaataactt ataacggacg tattgttcct gaatatagtg
     1801 tttatggtgg tattacttgg agcggtttct tactaccaca agatcgtgat gatgatactg
     1861 ttgtagatga agaagattct tgcccagatg aatttggtca tattagaaat ggtggttgtc
     1921 cattcccaga accagatatt gatggcgatg gattttgtga tgcttgggtt tctgaaaaag
     1981 gtttattaga tatttttgtt gatgtttgta aaggtgttga tgaatgtcca aatgaaaagg
     2041 gagaaggtaa agacggttgt cctctagata atccagatac cgatgccgac ggcatttgtg
     2101 atccttgggt aaaacagaaa aagatgagta agcgctttaa gggtgtatgt aaaggtgtag
     2161 atagttgccc aacacaagct ggtcctacaa cgaataaagg ttgtcctcaa gataatccag
     2221 acgtagatag cgatggcatt tgtgatccat gggtttctca aaaagataaa ctttctgatt
     2281 tcgcaaatct atgtaagggt tacgataatt gcccaggtga agctggccct gctgcaaata
     2341 aaggctgtcc atggccagat ccagatactg atggcgatgg tctttgtgat gaatgggtga
     2401 tcaaacagaa aatgggttac ttctttgaaa atccaacaga ctctactgtg aaaaaatgta
     2461 agagtgttga taaatgccca tatgaatttg gtcctgctga taacgaaggc tgcccatttg
     2521 ataatccaga taccgataac gatagcgttt gtgatcaatg ggttgctttg aaaaagcaac
     2581 aagatcgttt tgtaaatgtt tgtacgggtt ctgataagtg cccaacagaa gcaggtttac
     2641 taattaataa cggttgccct gtggaagatc ctgatattga taaagactcc ctctgtgctc
     2701 catgggtaaa agaaaaagga atgcaagatc aattcaagac tatatgtaaa ggaattgata
     2761 actgtcctac agaatttggc cctaaattta atggcggttg cccaattgat gatccagatc
     2821 cagataaaga tagtgtctgt gcttcttggg ttatagaaaa aggaatgcta gatcaattta
     2881 aggatgtatg taagggttct gataagtgtc ctacagaatt tggtcctgtc tttaataacg
     2941 gatgtccagt ggccgatcca gatccagata aagatagtgt ctgtgcttct tgggtcactg
     3001 aaaaaggtct tttgaaagaa tttgaatccg tttgtaaggg cgctgataga tgtcctactg
     3061 aagtcggtcc tattgaaaat aaaggttgtc ctttagaaga tcctgactta gataaagatg
     3121 gtgtctgtga tccttgggta actcagaaga agttgctcag caagtttgta aatacttgta
     3181 agggtctaga tcgctgtcca ttagattcag gctctgtaga atccaatggc tgtcctgtta
     3241 aggaaattga agaaaatgtg aaactggaag gtgttacatt taagagcggt agttcaattc
     3301 ttgaatcaaa tgccaagaaa gttcttaagg ttattgctga acagttactt gctccagaaa
     3361 atgcctctgt aaacattgaa attcacggtc atacagataa tgtgggtcgt cctgctaaaa
     3421 acaagaaatt gtctttacaa cgtgctcaat cagttgtaaa ctatcttgct tctcaaggtg
     3481 ttccaaagaa acgtatgaaa gcctttggtc atggagatga agaaccagtg gccgataata
     3541 aaactaagga aggtcgtgaa caaaatcgac gcatagaaat gcatcgagca gagtaatttg
     3601 aaaaaataga tcgtaatact aggctacaat gaacctttag attcattttt gcctagtatt
     3661 ttctagtata aacaaatagt tcattttatt actaaactct aatacaaaag tattagagtt
     3721 ttttttatct ttagcacagc aacttgtttt aggattttaa aatggaaact ctatatctta
     3781 ttgcattaat ccttattttt actcttgcta tttttgattt atttgttggc gtaagtaatg
     3841 atgctgtaaa cttcttaaat tcagcggttg gttcaaaagc ctttaagttt aaaacaattc
     3901 tttttatagc ggctctaggc atttttgtcg gagcctcttt ttctagtggg atgatggata
     3961 ttgctcgtca tggtattttt caacctcagc atttttactt tgtagaaatt atggccattt
     4021 ttatgggtgt gatgattacg gatgtgatct tacttgatac gtttaacttt tttggaatgc
     4081 ctacttctac tacagtttca atggtctttg aactactagg aggagctttt gctctttcat
     4141 tatttaaaat catacaaagt aacggtgatt taagttttgg tatgttgatt aatacagaaa
     4201 aagcattatc tgtcattttt gccatatttg tttctgtagc gatcgccttt acttttggta
     4261 ccattgtcca gtatattacg cgcttgatgt tgacttttga ttttcaaaaa agattgaagt
     4321 attttattgg cctttttgga ggcctttcat ttagctccat tgtttacttt attttattca
     4381 aagggttaaa agactctgtc atagcaaaat tgcctttttt tcattttata aaagactatt
     4441 ccatcgaact ttttataggt gcttttatct tctttgcttt gttttcatac cttcttcttt
     4501 ttcttaaaat taatgttttc aaatggatag tcgctttagg tacattctct ttagctctcg
     4561 cttttgctgg taatgattta gtcaatttta ttggtgtccc tttagcagct ctttcttctt
     4621 ttcaagattt tagtgctaat ggaacttcag tagatacttt tttaatgact tctttacaaa
     4681 gctctgctag tggggatagc tggattttag tagtcgctgg cgttatcatg gttattgctc
     4741 ttttgacctc taaaaaagca cataatgtga ttaaaacttc tttatctctt tcttctcagg
     4801 gtgaatccga tgaacctttt ggaaccaatc ctgtcgcaag agccgtagtg cgttggagtc
     4861 attctattgg ggaatctatt gctcacatgc tccctaaaag acttctaaaa tggacacatt
     4921 cccgtttaga tgactcgaaa ctcacattac atgcagaagg ggcttctttt gatttagtgc
     4981 gtgcttctgt taatttaatg ctggcgagct tgttaattgc tgcaggtact tccttaaaat
     5041 taccactttc tactacttat gtcactttta tggtggctat gggtacttct cttgctgata
     5101 aagcgtgggg tagagatagt gctgtatatc gtatttctgg cgtactttct gttattggcg
     5161 gttggttctt tacagccttt gcagcattta ttagtgcttt tatcgtatgt gctttaattt
     5221 atatcggtgg cttcccaatt ttattttctt tctttgtagt agtaatcttc ttattagttc
     5281 gttcttcaaa acgcttcaag aaaaaagctc atgctaaaga tttagctcaa gaacgtttcc
     5341 ataagatcat gattagtaac gatccaaaag aaacccttga attagttaga gatcattata
     5401 aagaagagtg gggatggcat ttaatttggg tggaagatcg ttatagagat cttattacgg
     5461 cttttattac agaagattta accaaattac gcatggtcaa tcataaaatt gacgatcaaa
     5521 aaaatgtggt aaaaagatta aaaaggcaag gtactcttgc ttctcgaaag atgagttcgg
     5581 atgacgctat tgccaaaaca tttttcttga tacaagccaa tgattatgca agtgatatgg
     5641 ttttccattt agatcgtatt tgtgacccaa gcttgcagca cgtagacaat cattttgctc
     5701 caataagtaa gaatcaaaag aaagtattag attctctatg gtctgaaatt acagattata
     5761 ttgaagattg cggacgtatg attttctctg ggatatacga caattgcatg gatttggaag
     5821 aacgagggcg tatccttagt gaaaaagtca tttctattag aaaagaacag acggatttga
     5881 ttaagaatca gaaatccaat acacgtgcaa gtattgtttt ccttactatt ttgtacgaga
     5941 caaaatctat tgtcgatacg tcgttatttt taacaaaatt cagtagaaag ttgatgagct
     6001 aatgacaatt cttgatcaaa ttttaacaga agctaatctt gataatcatg ctttagtcga
     6061 agcctcttct aagcaattat ctcataagac ggttcaaaaa gcaagaactg gtaaacgtcc
     6121 tctttctaaa aagatgaaag ttctcgtgac agaagcttta aacaaaactt tgcaaccaga
     6181 aaaactttat aagaaagaat atttgtttcc taataattta tctgaagaat tagaagaaaa
     6241 caatgaatca aactgatata ctgttgtata aaaaatgatt ctatcacgtt gttgattaat
     6301 cattttaagc ttaccaagtt gaagtcattt tataaaaaaa gactcatttt aatgagcctt
     6361 tttttataag tagattgtat agagttttta actaatgacc aaaagaattt gttattgagc
     6421 ggtcattggt tttagagagt taaaaatatc tttatatgat ttttcatcga ttcgcttata
     6481 accagtggtg attgtaccga atgtataata agctgaatcc cctttaacgg tcatataaaa
     6541 ttccatatct aaagaatttt taccgcgtaa ggcttcatct ttatattcag cgttaagaga
     6601 accattaatg ttagaaaccg tatacttacc atcactggtt tgtttaactt caaagatcat
     6661 attttcttct cctaaccaga aaccttggta gttaggtttt tctttttcag aacagccagc
     6721 taggaatagg acaaaaagaa tggatgtaaa aaaaaggatt tgttttttca taagaaatct
     6781 ccttgctata aaatagtatt ttttatattc acagatgcgt tatttatact tactacctct
     6841 tttttttgtc ataataatag ccatttttgt ttggcaaaat acaacaaaat ctttttcgaa
     6901 agattctatt atagaaagtt cctcttcaat attcatttct tctgattctt ttaaaactta
     6961 ttcctttagt gctgatgatg tacatcgttt gctacttgca agaacaaaac aaaaaaaggg
     7021 ggtttatttg gataaaatgg aaccttcatt agattcttta gctttggata ttgtatgtgt
     7081 atttcatgaa gtcatggggg atagctatgt gccaactatt acgagtgcta atgattatcc
     7141 ttatcatgcg agattctcta agcattatta taacaaagcc attgatttta ggacaaagga
     7201 tttaaattca agacaaaaaa aagagctttt agaaaaagct caaataagaa tttataaaaa
     7261 agggcgtgtg cttttggaag tcaatggaaa atcaaacgag cacttacatt tagagctctt
     7321 aaattaaggg gcttctctac agcaacgaaa tcctacataa gggtattggt tttgagggta
     7381 gaaactgaat ttattctctt tacattgagt taagttttgc ccgttccaag cgccgccagc
     7441 cactaaaaat gaattggcct tttgagaact aggggtactt gtccattccc aaagattacc
     7501 attcatatcg ttcatacccc accagctacg gcattggtct ttacggccag aacgcataac
     7561 tttttttgta tttgtattgc atttgaacgg gtgatattta tttccataag aataggtgaa
     7621 tttgtctttt cctcggcatg cggcttgcca ttcatctata gagcaaagcc ttttcccttc
     7681 tttttcgcat aaagccatgg cttgttcatg agaaaccatg tcacgaggaa tttcatctgc
     7741 cacatttgga tattcataag catctacaca gatttctttg ccattgacag gaacagaaaa
     7801 tccatttttg ccacagcgat tatcgcttgc catatcaaaa gagccttttt gccatggagt
     7861 tgaattacct aaagaatctt cggccttaaa aaatacatcg cctgttttat tatactcgac
     7921 acctgtacta gcatcttgtt tttgagtggt gtcgttaact gaaatccatg ttttgcattt
     7981 gatttcatcg cacttgactt tgagtcgaat aggctcataa taacgacctg gaggaggtgt
     8041 taaaaaggct tctggaggga ttgtatctaa ttttctgata ctgtcttgta tgcgtatttt
     8101 ctctaaagaa tctcttttta aagcttcggc gatttcaatg gcttttattg aatcttgatt
     8161 gatcttaggg gaaatagaat cttgcttttt gataataggt actttcgttt gagaaaggct
     8221 atcggctttt cttaaactat ctgctacgcg aatactatcg gcaacgcgta agctatcgtt
     8281 taatgagttt tctaatactt ttaggctatc taatttttta aggctatcag ctctttgctt
     8341 ttgccataat aaaagttctg tttgagcttt ttgtttattt aattcattct cttttatctt
     8401 ttgtaattca cactgaacta aaaacaacgt gattaataat aaaagcatta aaacaagaat
     8461 gaccgttttt cttcttttag atctttttac ttttttagct tcattttttt cttttggact
     8521 agtcatcttt agtgctcctt tctctagtca tcattctttt cggcttttac ttcttcaaat
     8581 aagatacgcc aatgtctttt gatttcttct tctaattctt tgatgctgag ctttcttcta
     8641 agagtacttc tataggcaat agaaataata cctgtttctt ctgatacgac aatcaccatg
     8701 gcgtcacttt cagcggcaag cgcttttgca gcacgatgtc gcatacctaa actagattct
     8761 tgatcagaag atgtggatgg taatggaaga atacaaccag ccgccacaat atttttatga
     8821 ttcataatga cagcaccatc atgtaatgct gaatttggaa agaatatgga gcgtaacagt
     8881 ctgcaactaa tgatggcgtt taattgttca cccgtatcag catagcttct taaacccact
     8941 cttttttcta atacgatgag ggctccaaaa cgatttttag ctaagtcttg tacagcagag
     9001 acaagagctt ctgtggtttc atttaagccg ctagaatgga aaaaaatgct tctccagtta
     9061 gtttgactga ctccttgacc aattcgagtt aaagcaccac gaatttctgg ttgaaaaaga
     9121 atcacaacag ctacgacacc taatgtagcg acattactaa gcatccaaac taaagtatgg
     9181 agttcccacc attgagcgag aaaccaagct aataaaagga gagctcctcc aaaaaacatt
     9241 tgaacagctc gtgtgcccct aaaaagtaag aatacgtaat aaagaataat cgatattaaa
     9301 agaatatcga gaatatctgc catacggaca tcaataacac caaataactt aaatagaatc
     9361 ataactccgg aacgcctcca aaagcttgaa cgattcaaaa gtagaagcca catcatgtac
     9421 tcgaacaatg gtggctccac caagagcggt aataaaagct gaaactactg aagggatgaa
     9481 gcgatcacta gtttctaaag catggatttt accaatataa gattttctag atagcccaaa
     9541 caatataggg aaatctaagt ttgaaaaatc ggcggcagaa gaaat
//
LOCUS       DAOOBB010000062         9553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_8919_length_9553_cov_4.608023, whole genome shotgun sequence.
ACCESSION   DAOOBB010000062 DAOOBB010000000
VERSION     DAOOBB010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 9553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9553
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8919_length_9553_cov_4.608023"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="PLT31_04285"
     CDS             <1..425
                     /locus_tag="PLT31_04285"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014544907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPW94389.1"
                     /translation="HTNWQSIPSISPTRGRYASPFGSRKHPIKGIVRMHNGIDIANDR
                     WTPIYASADGVVQRAQFSSSLGNFVAIDHGNGIETKYGHMQAYMVRSGQFVKRYQIIG
                     YMGNTGLSAGPHLHYEVWVNGKAVNPVHYILPNDHAIE"
     gene            512..1903
                     /locus_tag="PLT31_04290"
     CDS             512..1903
                     /locus_tag="PLT31_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94390.1"
                     /translation="MGCPLFNEFLPDPQDTVDLYGEFIEIRLGSDFSVKDTLYIQFED
                     KIEYAFTQIKASRLLLHRDTSTCKSSELLDCRPLPFPALPNSRSSFWSLRSLNCVDSA
                     ALPIPKNGKSFQRYSSEKDSWVYVSPSPGEANSQYESGIKDCAIRIKQAKYLEKKWQI
                     QLQVLNCDSSLVEWTILPLKYLNLDESKTMTITDSLWIFSRYEGESLLFKAALSLDEN
                     IQNNQVDTLLFLHESPPVYLTEVHHCPEEPTPEWIEIYNQENRALPLVHFGLGERGLI
                     SSSVEDSIDSHQSLILTKDTLAFIQSKGLADLFFKQVALGYLKNTSDTIFLTYKNTVL
                     DSTIWNKKTGIECPSGFNPKTGRAENTPGFQGRGLKIQAKTSPFSFKINTRVISKSID
                     ENSLQILIESEEGVLIELLSGQGNLLWHMKNNALNHNWISIPIKQKGQLGPNFIRLSV
                     GHYEKVVGVVLRP"
     gene            1869..2633
                     /locus_tag="PLT31_04295"
     CDS             1869..2633
                     /locus_tag="PLT31_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94391.1"
                     /translation="MKKWWVLFCVLSFSSVCAENPSFPSSMYPGFWGFMGSTLESGAP
                     SEESSPARRTDSLWLSAFWNNLNAVEDCDQFGFSSEWGRSFYRSAFLFSLLSLDSIYR
                     AVSFSGEIALHKSRFNIGIGHVWRTEWVPGIDSWATHLTKIGLHLKLVGGFSFGGLYI
                     INSEEKKRWWGGLHWGSVSGTRFFLELGQGILNVGQSIDFGALRFQSAFSYPGPQISV
                     GLILSFRNLLLGAGIYKNSNQFSSNSFHLNWNIFRK"
     gene            2778..4034
                     /locus_tag="PLT31_04300"
     CDS             2778..4034
                     /locus_tag="PLT31_04300"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPW94392.1"
                     /translation="MMDMFTLISIILVLVIVCIFLSYKLLALQKYLSNKKLIEVFSQE
                     FIASLSEDFSFLYVSPSSVNTIEMPPSLLKGRYLKEFVHPDDLDLLVRSLEAPISDDR
                     IKTAVFRCRHGSEGWCWVEMYGKKIRWESKKKILVCYFKNVDSVIKLRSDHNSIEKRL
                     QIFLRLSSDIVWEIDVEKRELSILTPVVFERHRVPSRTPGRVSMDEIMPAEDLRLVEN
                     VINARVQHFSEYGRDVPSPEEIFIRLYGIDESRVWYSVRGLLDKNINGRLMLYGSGHL
                     VDHALINKTSPEIKTGLFDSIMSLPYVRVFWVDKDQVYLGCNQTYASDLGQYNPSKVI
                     GEHMQSFSVNAALGSILKDNTLRVIKTRQACSGKTELFIGPDSVRHLLFYSFMPLEDE
                     SKSVSAVLGIYLISNTDRITPLNLNM"
     gene            4047..4469
                     /locus_tag="PLT31_04305"
     CDS             4047..4469
                     /locus_tag="PLT31_04305"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPW94393.1"
                     /translation="MKKRILFLLLGVFISFGCSESSSEKEAKSKPVVKKEEPIQTISY
                     KTIEWNEAIQLNQKGETLFLDVRTPSEVAEGDVSGALNIPVQELDRRYSEIPKDKKLL
                     VYCRSGRRSAAAASMLIQAGYKEVYNIRGGFSQAPAGL"
     gene            4476..5510
                     /gene="ribD"
                     /locus_tag="PLT31_04310"
     CDS             4476..5510
                     /gene="ribD"
                     /locus_tag="PLT31_04310"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HPW94394.1"
                     /translation="MSTDSKYMCLALQQAHFAIGVSRPNPPVGAVVVSNGIVVGKGFT
                     QAPGNAHAEVMAIKDAGPKALGADLWVTLEPCCHYGRTPPCTQAIGEAGIRRVFYSHP
                     DPNPAVRSKSKEILEVAGIEVFEGILSEETNRFYEAYDYFVRNQRPFVEVKVAQTQDG
                     FIAGKNRERLMITGDEANHWTNSLRSISDAILIGGGTLEADDPLLSLRGLEGNSPERI
                     VLVGKRLLSSRYRMFHEGKKPIIYSEVPQKEIEEIAEVRLLRGNNFLSHWTQIIDDLS
                     NRGMHRLLVEAGSQLSKLIIDAQVYNRFHVWVSPKRIGEGLPWALTIEMDPSEKDQHI
                     SFVNHKFNGK"
     gene            complement(5541..6365)
                     /locus_tag="PLT31_04315"
     CDS             complement(5541..6365)
                     /locus_tag="PLT31_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94395.1"
                     /translation="MEGILIIVIFAVVNIYSSWQKQKKKFEAEKRGKESSSDPLAPIP
                     LDPLQELLRKFDEAQKKYSQNSEDVPNEMEDFEEERFEDDFEGEVIKEEPEIKPVVSE
                     TPPQESPQKKFVLNETHSNPVPQNQPSFAPTSLNQSEPLNSQINSTPESKTILEKAAY
                     FAITCNTISEDLLIEEFMIGNAKAASLIKQLEYLGICGKNMGELNRIVLVKNENDLIN
                     ILKRASDLNLSQNPVSFEVRHKRKFVFSKEEAEKGVIWSKILDEPRFKKHWTPQSR"
     gene            complement(6369..7352)
                     /gene="floA"
                     /locus_tag="PLT31_04320"
     CDS             complement(6369..7352)
                     /gene="floA"
                     /locus_tag="PLT31_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547008.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HPW94396.1"
                     /translation="MNPILIIGIIIAAIIALVLLAFIGKFFSLWLQALFSKAGVSIFQ
                     LIGMRLRKVPPQTIVEARILSCKAGIPVDTNLLEAHYMSGGQVLRVVQALIAANKANI
                     QLNFKDATAIDLAGRNVLEAVQMSVNPKVIQTPKVSAVALDGIQLHAVTRITVRASIQ
                     KLVGGAGEETVLARVGEGIVSSIGSAQSHKEVLENPNMISKKVLASGLDAGTAFEILS
                     IDIADVDVGQNIGAILETDRAEADKKIAQAKAEERRAMAFAAEQEMKAKVMEMRAKFV
                     EAEAQIPMAMASALRDGKLGVMDYYNLKNIEADTQMRRELGGTQESNSSDK"
     gene            complement(7370..8851)
                     /locus_tag="PLT31_04325"
     CDS             complement(7370..8851)
                     /locus_tag="PLT31_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HPW94397.1"
                     /translation="MKTQHILFLLLFFFTSISFAEATTVKENSPSSSKKAVWIPLEGD
                     VDQGMVNYLGRALADASKLKPDVIIFEINTFGGSLDAAFSIVDTILSIKNTKTVALVQ
                     KKAISAGSLIALASQELYMLEGTTIGDCAPIVQGTDGTPTIVGEKIQSPLRAKFRNLA
                     QRNHYPELLSAAMVTPELEVLELLHQDSILIIEGKNYAKFSEEEKKFWGTPKVLVQEG
                     ELLTMTEKEALELGFSKATVQSKSQLEGLLEITSSEVIESTTSEKISRFIAAISGLLL
                     IIGFGALYLEFKTPGFGLFGIIGIIAIAIVFLGQYASHIGSYLPLILLVIGILLFVVE
                     ILIMPGTFLFGVAGIAAMMIALVMTFDISQLPSFLSSGSSFDASPWLYGLFFIFTCAI
                     IALIFPIIVSKYIVPLLPEGYTPMLKADLAEALSPTESIENIHIGSIGIAKTFLRPVG
                     HADFDGQLLDVQTRGEIIEPGEQIEVTLVQDSRLWVQKHTKNS"
BASE COUNT         2821 a         1660 c         1912 g         3160 t
ORIGIN      
        1 aacacacgaa ttggcagtct ataccttcta tttctccaac aagagggcgt tatgcttctc
       61 cgtttggttc tcgaaaacac cctattaagg ggattgttcg catgcataat ggaatagata
      121 ttgctaatga tcgctggact ccaatttatg cttctgcaga tggtgttgtt caaagagctc
      181 aattcagttc ttctttaggt aattttgtgg cgattgatca cggtaatggg attgaaacaa
      241 aatatggtca tatgcaagct tatatggtaa ggtctggcca gtttgttaag agatatcaaa
      301 tcattggtta tatgggaaac actggtcttt cagcaggccc tcatcttcat tacgaagtgt
      361 gggtaaatgg gaaggcggta aatccagttc attatattct ccctaacgat catgcgatcg
      421 aataaatagg tggtttaaag cattctaatt gcttttccca taataaggtt tccaaaaaat
      481 gttgttatcc ctttcgtgcg gtttcgcact gatggggtgt cctttattta acgagttttt
      541 accagatcct caggatacgg tagatcttta tggtgaattt attgaaattc gattagggtc
      601 tgatttctcg gtcaaggaca ctctttatat tcagtttgaa gataaaattg aatatgcttt
      661 cacgcaaata aaagcttcta ggcttctttt acaccgagat acctctactt gtaaatccag
      721 tgagcttttg gattgtaggc ctctcccttt tcctgcttta cctaattcca gaagttcttt
      781 ttggagttta aggtcattaa attgcgtcga ttctgctgct cttcctatcc ccaaaaatgg
      841 aaaatcattt cagcggtata gttctgaaaa ggattcttgg gtttatgttt ctccgagtcc
      901 aggagaagca aatagtcaat acgaatcggg tattaaagat tgtgctattc gaattaaaca
      961 agcaaaatat cttgaaaaaa aatggcagat acaactacag gttttgaatt gtgattcgtc
     1021 tttagttgaa tggactattt tacctttgaa gtatcttaat ttagatgaat caaaaacgat
     1081 gactatcacc gattctctct ggatattctc tcgctatgaa ggagaatctc ttttatttaa
     1141 ggcagcactt tctcttgatg aaaatattca aaataatcaa gtcgatacgt tactgttttt
     1201 acacgaatct ccaccagttt atttaacaga agtgcaccat tgcccagaag aacctactcc
     1261 tgagtggatt gaaatataca atcaagaaaa tcgagcttta cccttagtcc attttggctt
     1321 gggagagcga ggtttaattt catcttccgt tgaagattct attgattctc atcaatcact
     1381 cattttaaca aaagatacgc ttgcttttat tcaatcaaaa ggattagcgg atcttttttt
     1441 taagcaagtg gctttaggtt accttaagaa tacgagtgat actatttttt tgacttataa
     1501 aaatacagtt ttagattcta ccatttggaa taaaaaaaca ggtattgaat gcccaagtgg
     1561 ctttaaccct aaaacaggaa gagctgaaaa tacccctggt tttcaaggaa ggggcctaaa
     1621 aattcaggct aaaacatctc ctttttcatt caaaattaat acacgagtaa tttcaaaatc
     1681 tatagatgag aattcattgc aaattcttat agaaagtgaa gaaggtgttt taatcgagtt
     1741 actttcgggg cagggaaatc ttctttggca tatgaaaaat aatgcactaa atcataattg
     1801 gatttctatt cctattaaac aaaagggaca attaggccca aattttattc gtctttctgt
     1861 ggggcactat gaaaaagtgg tgggtgttgt tttgcgtcct tagcttttct tcagtatgtg
     1921 cagaaaatcc ttctttccct agttctatgt atccaggctt ttggggtttt atgggaagta
     1981 ctttagaaag cggtgctcca agtgaagaaa gttctcctgc aaggcgtacg gattctttgt
     2041 ggttaagtgc tttttggaac aatttgaatg cagttgagga ctgtgatcaa tttggattca
     2101 gttctgagtg gggacgatct ttttacagga gtgcttttct attttcgtta ttgagtttag
     2161 attcaatata tagggcggtt agttttagtg gagaaatcgc ccttcataaa agtcgtttta
     2221 atatagggat tggtcatgta tggcgtacag agtgggttcc tggaatagat tcatgggcaa
     2281 cacatcttac aaaaatagga cttcatttga aactagtagg aggattctca tttggaggtc
     2341 tttatatcat taattctgaa gaaaaaaaaa gatggtgggg agggctacat tggggttctg
     2401 tatcagggac tcgtttcttt ttggaattag gtcaaggtat cttgaatgtg gggcaatcta
     2461 ttgattttgg agcacttcgt tttcaaagtg cgttttctta cccaggaccg cagatttctg
     2521 ttggtttaat tctttccttt cggaatcttc ttttgggagc aggtatttat aaaaattcca
     2581 atcagtttag ttctaattct tttcatttaa attggaatat ttttcgaaag taatatagat
     2641 cacataaata caacgttttt tttgttatga caaaggttta agtaaaagaa ttattctaaa
     2701 ctttggtttt gtggtaaaaa gtttattaga taataataat aagctgctgt gtgtttagat
     2761 cctgtaagga gaaatgtatg atggatatgt ttactctaat ttcaattata ttggtcttgg
     2821 ttatcgtttg tatttttttg tcttataagt tactagcgtt acaaaaatat ttaagtaata
     2881 aaaagctaat agaagtcttc tctcaagaat ttatagcctc tctttcagaa gatttttctt
     2941 ttttatatgt gtcgccttca tctgtgaata caatagaaat gcctccatca ttacttaagg
     3001 ggcgctatct aaaggaattt gttcatcctg atgatttaga tttattagtt cgttcactag
     3061 aagcaccgat aagtgatgat cgcataaaaa cggctgtttt tagatgtcgc catggctcag
     3121 aaggttggtg ctgggtggaa atgtatggaa agaaaattcg ctgggaatcc aagaaaaaaa
     3181 tactcgtctg ctattttaag aatgtcgatt cagtgataaa attgcgctca gatcacaata
     3241 gtatcgagaa aagattacaa atatttttaa ggctttcttc cgatattgta tgggaaattg
     3301 atgttgaaaa aagagagctg tctattttaa ctccagtagt tttcgaacgt catcgagttc
     3361 catcaagaac ccctggtcga gtatctatgg atgagatcat gcccgcagaa gatcttcgac
     3421 tagttgaaaa tgtgattaat gcccgtgttc aacatttttc tgaatatggt cgggatgtcc
     3481 cttcgccaga agaaattttt attcgtttgt atggtatcga tgaatctcgt gtatggtatt
     3541 ctgttcgagg gcttcttgat aaaaatataa atggccgact catgctctat ggctctggtc
     3601 atttggttga tcatgctcta atcaataaaa catcacctga gattaaaacg ggtttatttg
     3661 attccatcat gtcattaccc tatgtgcgcg ttttttgggt agataaagac caggtttact
     3721 tagggtgtaa tcaaacgtat gcttctgatt tagggcagta taatccttct aaagtgattg
     3781 gcgaacacat gcaatccttt tcagtgaatg ctgcattagg atccatctta aaagataata
     3841 cactaagagt gattaaaact agacaagctt gttctggaaa aacagaactt tttattggcc
     3901 cagattctgt gcgtcatctt ttattttaca gtttcatgcc gttagaagat gagtcgaaat
     3961 cggtttctgc agtacttggg atttatttga tctctaatac tgatagaatt acacccttaa
     4021 acttaaatat gtaatgtgag atcaatatga aaaaaagaat tcttttttta ttgttaggtg
     4081 tttttatttc ttttggttgt agcgaatctt cttctgaaaa agaagcaaag agtaaacctg
     4141 ttgtaaagaa agaagaacct attcagacta tctcttataa aacgattgag tggaatgaag
     4201 caatacaatt aaatcaaaaa ggagaaactc tatttttaga cgtgcgcact cctagtgaag
     4261 ttgctgaagg tgatgtttct ggagccttaa atattcctgt tcaagaatta gatcgccgtt
     4321 attctgaaat accgaaagat aaaaagctat tagtttactg ccgttctggt cgtcgtagtg
     4381 ccgccgctgc ctctatgctt atacaagcgg gttataaaga agtttataat atccgtggcg
     4441 gtttttctca agcgcctgct ggtttgtaat ttttaatgtc aacagattct aaatatatgt
     4501 gccttgctct tcagcaggca cactttgcta taggagtcag tcgtccaaat cctcctgttg
     4561 gagcggttgt cgtttctaat gggatagttg ttggaaaagg ctttacacaa gcaccaggta
     4621 acgctcatgc agaggtgatg gccattaaag atgctggtcc aaaagctttg ggtgctgatt
     4681 tatgggtgac tctagaacct tgttgtcatt acggtagaac gcctccatgt acacaagcca
     4741 ttggtgaggc tggtattcgt cgtgtttttt atagccatcc tgatccaaat ccagccgtac
     4801 gctccaagag taaagaaata ttagaggttg ctggtataga agtttttgaa ggcatattaa
     4861 gtgaagaaac aaatcgtttt tacgaggctt acgattattt tgtacgtaat caacgccctt
     4921 ttgtagaagt gaaagtggct caaactcaag atggtttcat tgcaggtaag aatagagagc
     4981 gcttaatgat cacaggagat gaagcgaatc attggaccaa ttctttacgc tctatttcag
     5041 atgctatttt aattggagga ggaacattag aagcagatga tcctctcctc agtttaagag
     5101 ggttagaagg gaattcacct gagcgcattg ttcttgtagg aaaacgcctt ctttcttcac
     5161 gttacagaat gtttcatgaa ggtaaaaagc ccatcatcta ttctgaagtt ccacaaaaag
     5221 agatagaaga aatagctgaa gttcgtttat taagaggaaa taatttttta agccattgga
     5281 cgcagataat tgatgattta tctaatagag gaatgcaccg attattggta gaagcaggtt
     5341 ctcaattatc aaaactgatt atagatgctc aagtttataa tcgctttcat gtatgggttt
     5401 ctcctaaaag gattggcgaa ggccttccat gggctttaac gatagagatg gatccgtcag
     5461 aaaaagatca acatatttct tttgtaaatc ataaatttaa tggaaaataa aaaagcacca
     5521 tttactggtg ccttttttta ttatcgagac tggggagtcc aatgcttttt aaatcgaggt
     5581 tcgtctaaaa tttttgacca tataacccct ttttctgctt cttctttact gaacacaaac
     5641 ttgcgtttgt gacgtacttc aaaagaaact gggttttggc ttaaatttag atcgctagct
     5701 cgttttaata tatttattaa gtcattctca ttcttgacaa gaacgatgcg attgagttcc
     5761 cccatgtttt ttccacaaat gcccaagtat tcaagttgtt tgattaagct agcggccttc
     5821 gcgtttccaa tcataaactc ttcgatgaga agatcttctg atatcgtatt acaagtaata
     5881 gcgaagtatg ctgctttttc taaaatagtc tttgattctg gggtagaatt gatttgacta
     5941 tttaatggtt cactttgatt tagagaagta ggtgcaaaag aaggttgatt ctgaggcact
     6001 ggatttgagt gcgtttcatt tagaacaaac tttttttgtg gactttcttg aggaggggtt
     6061 tcagaaacaa ctggtttgat ttccggttct tcttttatta cctcaccttc aaaatcgtct
     6121 tcaaatcttt cttcctcaaa atcttccatt tcatttggaa cgtcttctga attttgagaa
     6181 tacttttttt gggcttcatc aaatttacgt agtaattctt gcagcggatc caaaggaata
     6241 ggagcaagcg gatcagacga ggattctttt cctcgctttt ccgcttcaaa cttcttcttt
     6301 tgtttttgcc aactactgta aatgttgaca accgcaaaaa tgactatgat gagtattcct
     6361 tccatagatt acttatcgga ggaattggat tcctgcgtgc cacctaattc acggcgcatt
     6421 tgagtgtctg cttcgatgtt tttaagattg taataatcca ttacaccaag ttttccgtcg
     6481 cgaagagcag aagccatggc cattggaatt tgagcttctg cttcgacaaa cttcgctctc
     6541 atttccatca cctttgcttt catttcttgt tcagcagcaa aagccattgc acgacgttct
     6601 tctgctttcg cttgagcaat tttcttgtct gcttcggcac ggtctgtttc aagaatagca
     6661 ccgatgtttt gcccgacatc gacgtctgca atatcaatcg ataaaatttc aaaagcagtg
     6721 ccagcatcta aaccagaggc aagtactttt ttagaaatca tattaggatt ttctaacact
     6781 tctttatggc tttgagcgct accaatagaa gaaacgatac cttcaccaac acgagcaaga
     6841 accgtttctt ctccagcacc acccactaat ttctgaatgc ttgcgcgtac agtaattcta
     6901 gtgacagcat gaagttgaat accatcaaga gcaacggcgc ttactttagg tgtttggatc
     6961 acttttggat tcacagacat ttgcacggct tctaaaacat ttcgaccagc aagatcaatg
     7021 gctgtggcat ctttaaagtt taattggata ttggctttgt ttgcagcaat caaagcttgg
     7081 acaacacgaa gcacttgacc accagacata taatgggctt ctaaaagatt ggtgtctaca
     7141 gggatacctg ctttacaact taagatacga gcttctacaa ttgtctgtgg tggcacttta
     7201 cgtaaacgca taccaatcag ttggaaaatg ctaacgccag ctttagagaa taaagcttgt
     7261 agccagaggc tgaaaaattt accgataaaa gcaagcagca caagtgcgat gatggctgca
     7321 ataattattc ctataatgag aatgggattc attgtttctc ctgtagatgt tatgaatttt
     7381 tagtgtgttt ttggacccag agacgactat cttgaacaag ggtgacttcg atttgttctc
     7441 caggttcaat aatttctcct cgagtttgca cgtcgagtaa ttgaccatca aaatcagcat
     7501 gtccaacggg tcttaaaaaa gttttagcga tgcctatgga tccgatgtga atgttttcaa
     7561 tggattcggt aggggaaaga gcttctgcaa ggtctgcttt taacataggg gtatagccct
     7621 ctggaagtag agggacaatg tatttagaaa cgatgattgg gaaaatgagt gcaatgatag
     7681 cacaagtaaa gataaagaag agtccataaa gccaagggga agcgtcaaaa gaagaaccag
     7741 aagacaagaa actaggcaat tgagaaatat caaaagtcat tacgagagca atcatcatgg
     7801 ctgcaatccc tgcaacgcca aataagaaag ttcctggcat aattaatatt tcaacaacaa
     7861 ataaaaggat acctattacg agtaaaatta aaggtaagta gcttccgata tggcttgcgt
     7921 attggcctaa gaagacaatg gctatggcga taataccgat gataccaaaa aggccaaacc
     7981 caggagtctt aaactctaag tataaagcac caaagccaat aatcaaaaga agtccagaaa
     8041 tggcggctat aaaacgtgaa attttttcgc ttgtagtgct ttcaattact tctgacgaag
     8101 taatttctaa tagaccttca agttgagatt tactttgtac agtagcttta gaaaagccga
     8161 gttctagggc ttctttttct gtcattgtga ggagttcgcc ttcttgaact aagaccttgg
     8221 gtgttcccca aaacttcttt tcttcttcag aaaacttagc gtaatttttc ccttcaataa
     8281 tgagaataga atcttggtgt agcaactcca gaacttctag ttctggcgta accatagccg
     8341 cgcttaagag ttctggataa tggttacgct gtgctaaatt tctgaattta gcacggagtg
     8401 gcgactgaat tttttctccc acaatggttg gagttccatc ggtaccttgc acaataggag
     8461 cacaatcccc aatggttgtg ccttcaagca tataaagctc ttggcttgct aaagcaatta
     8521 aagagcctgc actaatcgcc ttcttttgaa cgagagcaac cgtcttagtg tttttgatgc
     8581 taagaatcgt atctacaata gaaaatgcgg catcaagact tccaccaaag gtattgattt
     8641 caaaaataat gacatcaggt tttaatttag aggcatctgc taaagctcgc cctaaataat
     8701 taaccatccc ttggtcgaca tcgccttcta aaggaatcca gactgctttt ttcgaagacg
     8761 agggagaatt ttctttgacg gtagtggctt cggcaaaaga aatacttgta aagaaaaaaa
     8821 gtaaaaggaa tagaatatgt tgggttttca tactatgaat gtataaaatt acgggcctct
     8881 ttgtgacgca aaatgggtgg ttttttccat tttttcattg ggtctcaata agactgttat
     8941 tgattgaaat aactgttatc tcttcacttg gttgactatc cagtaacatg aacacttaaa
     9001 agtttacttt ttagtgagaa tgatcccctt gcgggacaaa aagctcatca ataacagtta
     9061 tttcaatctg agtcattgta ttgtattgat tgaataaaca gtgttgtatt tttcatttga
     9121 gcctgcgcta ttgaatttac aattcaaaat acgcttgtct ctgcatgaaa aaaactcagt
     9181 taactttaga ggggcatctt attttcatgc aaagcttttc aaaacgagct catcaataac
     9241 agccattccc gtctgctatg caactattgt tatttaattg actaggattg tttttttcat
     9301 ttgaatctgc gctatttgct ttctaagcaa attgacgctt gattcggtat gaaaaaaact
     9361 gggttcgttt agatgagatt ctcattgtct cttcatgaaa aaacttggct gggttagagg
     9421 ggcgtttctt tttcatgcaa ttacatttta agagaaactc gtcggcaaca gccatttccg
     9481 tctgctatgc aactattatg atttaattga ataaggttgt tttttcattt gaatctgcac
     9541 taattgattg cta
//
LOCUS       DAOOBB010000063         9508 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_8976_length_9508_cov_4.854861, whole genome shotgun sequence.
ACCESSION   DAOOBB010000063 DAOOBB010000000
VERSION     DAOOBB010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 9508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9508
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8976_length_9508_cov_4.854861"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3605
                     /locus_tag="PLT31_04330"
     CDS             <1..3605
                     /locus_tag="PLT31_04330"
                     /inference="COORDINATES: protein motif:HMM:TIGR03903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TOMM system kinase/cyclase fusion protein"
                     /protein_id="HPW94398.1"
                     /translation="DQGSTKDDQLYAVFEFVEGETLQELLKRKGALPVRQLKVIMCQV
                     LEALVYAHQKGVIHRDLKPGNIMVHSIQDHTYAKILDFGMGGFLEESGVDYQTLTLPS
                     DALGTPSYGAPEQLRGERPSSKSDLYSWGLVFLECLTGEPVMKGASVADIIHQQLLLD
                     DIVLPAGIASHPLSTLLRRVLAKRVEDRASDASAVLAELEKIHLDDLVGSFRSSVKEQ
                     SSYYVADETAEGILAGQDLQEVTLLCYAIELTSNQEKKTVPENRDFLFRSIKNNFADI
                     VSKYGGYAAGSLGDFHLCYFGYPRSSDMDASLAGRCALNLQKEMEVFHQKNLADSPFR
                     FNVRIGIHSGPILVERGAVPSGETSHDVLRLTALTPPGKIYLSHTSCERLIQRVNIEP
                     VTGLESFIERGVAALISEKSNPPHLFQQGLPFIGRIKERESIEKGFEKVKQGSTVSFL
                     IQGEPGIGKSSLIRSLYPLFQSEDSLLEEIQCYPEYKNNALYPIWQMIRRQINISEKE
                     NPEEAVKKLKDAFIMTKSDMDTFFPLCCLWLSLPTPAPYELKKLSFEKQKSVLFEGLS
                     HFIRYIASRKIVLVIEDLHWADPTTLEWIHFLLEKNDTTAMLILLTARPEFKSPFSKN
                     KVSTISLSELSTKEAQELVLSLTQDNPIASAIQSNILNRAGGFPIFIETLTQTIVEKV
                     STQNKTEEVLRSSFWGNIPNSLMDLLNAQIHQLHKSRETAQIASIIGRDFEYDLMAQI
                     SFLSEAALKEDLQELINQRIIEIKEIKNREVYTFKHALLKEAAYLSVTQAKKKEYHSR
                     IAEILEKNNSNPNEQYFMELSKHYAGALDYEKAISIGIDAATSFLNKASLLEAITLSF
                     EMLDWIDLYQNTKKEEFRLQIHRILTQALMGSHGWADPKVKKYLDISRSLLDEIGESR
                     HYGPILCSLMTYHYVSSNRKELKCISEEVMHHAVVISDIGLEIAGKMFYGLWAHGAGE
                     YEEAEACFEYALSHYDPVEHKNHGELLGIDTRAWSYATLALVKWFRNDSANAVLFSDK
                     GVAWARELNHIPSLCISLLYKANLCHYRGNREEALEIVEELNKYAETYNLPAYKAYGQ
                     IMLAWIKKNPEKAEAEIKMLEEMGCTAALSYYRSLLADTYADQGRYDLAVDNIKACLL
                     LCESNDEYYYQFELLRLEFLYLSKMTPPKTEEAAKVLEKAKTVAFSQGIICTNF"
     gene            3800..4615
                     /locus_tag="PLT31_04335"
     CDS             3800..4615
                     /locus_tag="PLT31_04335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMA_0021/BMA_0022 family TOMM bacteriocin"
                     /protein_id="HPW94399.1"
                     /translation="MNGTNDLMKFRTMYLKAIAESWRDTTGAFLKKLQQDPIGVLQSL
                     GDYEFPWDIHIDIKSVAPEDSLWSPEIAGGWVGKNAVICLWLPPPPENEDEYGRAWAA
                     YYDEFPSYLGAIQNSPRQANPASYGHSYAPGMGKANDFLEFSAVVMRLIALVWKDKQV
                     LREIQAIENTNKGVTIINKWLGFNMPWNMDVSLKLSGWSSSSSDEEKLCRWNSERKEW
                     PKPIQGKRPNSCRNSLQFYIPSRPIQAEKDKSIEAIALSAYNMTGDQYPFTCP"
     gene            4681..6876
                     /locus_tag="PLT31_04340"
     CDS             4681..6876
                     /locus_tag="PLT31_04340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOMM precursor leader peptide-binding protein"
                     /protein_id="HPW94400.1"
                     /translation="MSVKNMNDSGIYLGKGLQIFVLSPNKAIVVGEKNHFVISGEDQV
                     AVLELINQSYSETEILMALGTRLGELNVLSILHHFFHSQLCYRAAFPIDATCQDYICY
                     ENSNLLSDYNSFSIEFLEDSDNKGIIENLFEEEGLFSDQSHLESNSKLRVIVTKSLTH
                     LSLKEINRTQKKLNSPWLLLSLEGPDPMIIPFFNPVYGPCYQCFLFWYKVNHPVEQYI
                     LKKTGEMVSSKEVAQKMAVRELAKQAVIFIKNFLLGSRDVAFDVMSIDGETRICSLHT
                     LLKRPQCPCCGDSKYMKNQGFEAIQLENKKKGFVKDGGARLQDPLTTFHQFRNLISPL
                     VGPVSQFGPMPNRHTNERPVFLSGYRTCPSLDDIDAFSFQRICAGKGCSVEQAQVSAL
                     FEAVERFSSLYQGDEAIVKGCLHDMGPSAFHPNDLQLFSDAQLDSWTQEHLQKLGALK
                     VAKRYNEEDPLHWTPGWSLTYNCRRFIPFTYCFSDVSSHINSSYACYTGNGVAAGVCL
                     EEAILQGLFELVERDAVAIWWYNQIQRPAPNAEVLADSFYIKTKEEYKSAGWNMWVLD
                     LTHDLSIPVYAALAQSEKDGRFCIGFGCHLSSRLALYRALTELNQLFDPAGDLKTPWD
                     DHAMTSRDYLFPSGESASSRTYPLNQTGFLDDIQECLHLLNLANLELVVVNKTRPDTG
                     VKVAHVIVPGLRHFWPRFGKGRLYDVPLKLGWLKKSPYEKALNPVPLLV"
     gene            6891..7847
                     /locus_tag="PLT31_04345"
     CDS             6891..7847
                     /locus_tag="PLT31_04345"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPW94401.1"
                     /translation="MGKILKNETEICLFRDHIFGRDLRCSSVLKGNAVSRFHARISWN
                     GENWCLRDLGSRNGTYVNGRLLATEGVFYLNPGDVILFGDFEEKYLFSERTPPESCLI
                     YAGNDGVEKKVSLTHLLPLPSMERPVATIFAGKNGGYFLETESGELLPLQHAVPFFVE
                     EIKYTPFFENPPDESPQTDTSEMTGSSSAYINFLEILVSPDEESAEVRFHKGNEVFSL
                     NHKVHFYLLAYLARKRIEESEAQENTLKETKDDDNGWIDCEVISKELMMNRDHLGQQV
                     FRIRQEFKSIDPLIAEKIIDRQLRGKMRIGFPASKIQIKKMK"
     gene            7952..8278
                     /locus_tag="PLT31_04350"
     CDS             7952..8278
                     /locus_tag="PLT31_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94402.1"
                     /translation="MDKKSAIMAHMLTEQGGSVLVRCEGGFISRFTLSYEFEGIEFSK
                     HSGNISLGVNKSESVPIGAKNLFLKVEEMWGFGRSTIFIQQFAEPVQKCYKVYGTTLN
                     PKWIEI"
     gene            8354..9115
                     /locus_tag="PLT31_04355"
     CDS             8354..9115
                     /locus_tag="PLT31_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94403.1"
                     /translation="MSYDPFLKIYVEHEYSDFQLCPTLRLQPTKNTTHWLNQHKCISL
                     YGPGNVKISKHVDIHVEKPVILDFLGFCSDPNFPNYTLFENEEVESVPVYFCKTPRSN
                     NIKTEYLWEKWDYLFTENKIIRPYFIVKVQILPHVLHEQKISIRLSSKKVYAKYYIMG
                     EFAQKPTQIIDISYGHEKIHFNPVQETLPVSAQSFISDRPIPLQKGNRTHFQLQEKGS
                     SRILLSRLPDVNPKSLIEAKKSDGSVILIAETLVL"
     gene            9273..9419
                     /locus_tag="PLT31_04360"
     CDS             9273..9419
                     /locus_tag="PLT31_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94404.1"
                     /translation="MKKLFIFAACTLLIACASSSKKPLQYSSNDLFMQVQQAQDSLNR
                     IENN"
BASE COUNT         3065 a         1490 c         1863 g         3090 t
ORIGIN      
        1 tagatcaggg atctactaaa gacgatcaat tatatgcggt atttgaattt gtagaagggg
       61 aaactttaca agagctttta aaaaggaaag gagctctccc tgttcgtcaa ttaaaagtca
      121 ttatgtgcca agttttagaa gcattggttt atgctcatca aaagggagtg attcatcgag
      181 atttaaagcc aggcaatata atggttcatt ctattcaaga ccatacttat gctaaaattt
      241 tagattttgg aatgggtgga tttcttgaag aatcaggcgt tgattatcaa acactcactt
      301 taccttctga tgctttagga actccatctt atggtgctcc tgagcagctt cgcggggaac
      361 ggccttcatc gaaatcagat ctatactctt gggggctagt ctttttagaa tgtttgacag
      421 gtgaacctgt aatgaagggt gcaagtgtag cagatattat tcaccaacaa ttattgcttg
      481 atgatattgt attacctgct ggaattgctt ctcatccact atcgacttta ttacgtcgcg
      541 ttttagcaaa acgagttgaa gatcgagcct ctgatgcttc tgctgtttta gctgaattgg
      601 aaaaaattca tttagatgat ttagtgggtt cttttagatc ttcagtgaaa gaacaatcat
      661 cttattatgt tgctgatgaa accgccgaag gtattttagc ggggcaagat ttacaagaag
      721 taactcttct ctgttatgct atagaattaa catctaatca agaaaaaaag acggttccag
      781 aaaataggga tttccttttt agatctatta agaataattt tgctgatatc gtttctaagt
      841 atggaggtta tgcagcaggg agtttagggg attttcattt atgttatttt ggctatcctc
      901 gctcttccga tatggacgct tctctagcgg gaagatgtgc cttaaactta caaaaagaaa
      961 tggaagtttt tcatcaaaaa aacttagctg attctccttt ccgatttaac gttcgaatag
     1021 gaatacactc tggtccaatt ctagtcgaaa gaggagccgt tccttctgga gaaacgtctc
     1081 acgatgtttt aaggttaacc gcattaactc ctccaggaaa aatttatttg agtcacactt
     1141 cttgtgagcg attgattcaa agagtgaata tagagcctgt tacaggttta gaatcattta
     1201 tagaaagagg tgtggcggct cttatttcag aaaagtctaa tccacctcat ttattccaac
     1261 agggtcttcc ttttattggt cgaattaaag aaagagaatc cattgaaaaa gggtttgaaa
     1321 aagtaaaaca gggaagtaca gtctctttcc ttattcaagg ggagccaggt attggaaaat
     1381 cttctcttat tcgttctttg tatcctttgt ttcaatcaga agattcttta ttagaagaaa
     1441 ttcaatgtta ccctgagtat aaaaacaatg cgctataccc catttggcaa atgatccgtc
     1501 gccaaataaa tatatctgaa aaagaaaacc cagaagaagc agtaaagaaa ttaaaggacg
     1561 cttttataat gacaaaaagc gatatggata cttttttccc tttatgttgt ctttggcttt
     1621 ctctacctac tcctgctcca tatgagctaa aaaaattgtc ttttgaaaaa caaaaaagtg
     1681 ttctttttga gggccttagt cattttattc gatatatagc ttctcgtaaa atagtacttg
     1741 tgatagaaga tttgcattgg gctgatccaa cgacactcga atggattcat tttttattgg
     1801 aaaagaatga tactacagcc atgttgattc ttcttacagc aaggccagag tttaaatctc
     1861 ctttttcaaa aaataaagtt tcaacaattt ccttaagtga actttctaca aaagaggctc
     1921 aggaattagt tttgagtcta acgcaggata atccaattgc ttcagcaatc caaagcaata
     1981 tcttaaatag agctggtgga tttcccatat ttattgaaac attaactcag actattgtgg
     2041 aaaaggtttc gacgcaaaat aaaacagaag aagtattacg atcttctttt tggggaaata
     2101 ttcctaatag cttaatggac ttattgaatg cacaaattca tcaacttcat aagtctagag
     2161 aaacggctca aatagcttct attattggtc gtgattttga atacgattta atggcacaaa
     2221 tttcattttt aagtgaagcg gcattaaaag aagatcttca agagctaata aatcagcgta
     2281 ttattgaaat caaagaaata aaaaatcgtg aggtatacac ctttaagcat gctttattaa
     2341 aagaagcggc ttatttatct gtaacgcaag cgaagaaaaa agaatatcat tctcgaattg
     2401 cagaaatact tgagaaaaat aattctaatc caaatgaaca atattttatg gagctttcca
     2461 aacattatgc aggggctctt gattatgaaa aagccatttc tattggtata gatgcagcta
     2521 cttcattctt aaataaagca agccttttgg aagcgattac tctttctttt gaaatgttag
     2581 attggattga tttataccag aatacaaaaa aagaagaatt ccgtttacaa atacaccgta
     2641 tattaactca agctttaatg ggttcacatg ggtgggctga tccaaaagtg aaaaagtatt
     2701 tggatatatc aagaagcctt ttagatgaga ttggagaaag tcgtcattat ggccctattc
     2761 tttgctcttt aatgacatac cattatgtga gtagcaatag aaaagaactt aaatgtattt
     2821 cagaagaagt gatgcatcat gccgttgtga tttcagatat aggtcttgaa atagcaggga
     2881 aaatgtttta tggtttgtgg gctcatggtg ctggagaata cgaagaggca gaggcttgtt
     2941 ttgaatatgc cctaagtcat tatgatccag tagaacacaa aaatcatgga gagcttcttg
     3001 gaatagatac acgagcttgg tcttatgcaa cacttgcttt agtcaagtgg ttccgaaatg
     3061 attcagcgaa tgccgtttta ttttctgata agggtgtggc atgggcgaga gaattaaatc
     3121 atattccttc actttgcatc tctcttctgt ataaagcgaa tttatgtcat tatcgaggaa
     3181 atcgagaaga agctctagaa attgtggaag aattgaataa gtacgcagaa acctataatc
     3241 ttccagctta caaagcatat ggtcaaataa tgcttgcttg gatcaaaaaa aatcctgaaa
     3301 aagcagaagc agaaattaaa atgcttgaag aaatggggtg taccgcagca ctttcttatt
     3361 atcgatcttt attagctgac acttatgctg atcaaggtcg atacgattta gcagtagata
     3421 atattaaggc ttgtctacta ctttgtgagt ctaatgatga gtattattat cagtttgaat
     3481 tgttgagatt agaattcttg tatctatcaa agatgactcc accaaagacg gaagaagcgg
     3541 caaaagtttt agaaaaggca aaaacagttg cttttagtca gggaataatc tgtacgaatt
     3601 tttaaaataa atctctatag aagatattct atttggattt atattatcat gaagaaaaat
     3661 tacttcatat aattagaaat atttttaaaa acttgtatct ctttttcttt aaacgctata
     3721 ttttaataat agaacttgca ggggatgctc gcactttgta agtgaatatt tattaaaagt
     3781 gggcaaagag gggatgatta tgaatggtac gaatgattta atgaaattca ggaccatgta
     3841 cttaaaagca atagcagaat catggaggga taccacagga gcttttttaa aaaagcttca
     3901 gcaagatcct attggtgttt tacaaagtct aggagattat gaatttccat gggatattca
     3961 tatagacata aaaagtgtag cgccagaaga tagtttatgg agtccagaaa tagctggagg
     4021 ctgggtcggt aaaaatgcgg taatttgttt atggcttcct cctcctccag aaaatgaaga
     4081 tgaatacggg agagcgtggg ctgcttatta tgacgaattt ccatcttatt taggggcaat
     4141 acaaaatagt cctcgtcagg caaatcctgc ttcttatggg cattcgtatg ctccaggaat
     4201 gggaaaagca aatgactttt tagagtttag tgcggttgtc atgcgcttaa ttgccttggt
     4261 ttggaaggat aaacaagttc ttcgagaaat tcaggcgatt gagaatacca ataaaggggt
     4321 taccatcatt aataaatggc taggctttaa tatgccatgg aatatggatg tgagccttaa
     4381 actctctggc tggagttctt cttcttcaga tgaagaaaaa ctatgtaggt ggaattccga
     4441 aagaaaggaa tggccaaagc ctattcaagg aaagcgtccc aacagttgcc gtaactcttt
     4501 acagttttat attcctagca gacctatcca ggctgaaaag gataaaagta tagaagctat
     4561 tgcattaagc gcttataata tgactggtga tcaatatcca ttcacttgtc cttgatattc
     4621 tttcaataat aatcacatct aaaaactata agtgttttta gatgtgataa aaggtttttt
     4681 atgtctgtta aaaatatgaa cgattcagga atatatttgg gaaaaggtct tcaaatattt
     4741 gttctttccc ctaataaggc tattgttgtt ggagaaaaaa atcattttgt gattagtgga
     4801 gaagatcaag ttgcagtact tgaactaatt aatcaatcct attcggagac ggaaatcctt
     4861 atggctttag gtactcgttt aggggaacta aatgtacttt ctatcttaca tcattttttt
     4921 catagtcaac tttgttatcg cgctgctttt ccaatagatg cgacttgcca ggattatatt
     4981 tgttatgaaa actctaattt attgtcggac tacaattctt tttcgattga gtttttagaa
     5041 gatagtgaca acaaaggtat tattgaaaat ctttttgaag aagagggctt attttctgat
     5101 caatcacatt tagaatcaaa ttcaaaattg cgtgttattg ttacaaaaag tctaactcat
     5161 ttgagtttaa aagaaatcaa tcgaactcaa aaaaaattaa attccccttg gcttcttttg
     5221 tctttggaag ggccagatcc aatgattata cctttcttta atccagtata tggcccttgt
     5281 tatcagtgtt ttttattttg gtataaggtc aatcatccag ttgaacaata catactaaaa
     5341 aagacagggg aaatggtttc ttcaaaagaa gttgctcaga aaatggctgt tagagagctt
     5401 gctaaacaag cagttatctt tataaaaaac ttccttttag gttcaaggga tgtggctttc
     5461 gatgtcatga gtattgatgg tgaaacaaga atctgctctt tgcatacttt attaaaacgc
     5521 ccacagtgtc cttgttgtgg tgattccaaa tatatgaaga atcaaggctt tgaagcgatt
     5581 caacttgaaa ataaaaagaa gggatttgtt aaggatggtg gtgctcgtct acaagatcct
     5641 ttaacgacat ttcatcagtt tagaaatttg attagtcctt tagttggtcc tgtaagtcaa
     5701 tttggcccaa tgccaaatag gcatacaaat gaacggcccg tctttttatc tggatataga
     5761 acgtgtccta gtcttgatga tatcgatgcg ttttcttttc agcgtatatg tgcagggaaa
     5821 ggatgttccg ttgaacaagc tcaagtgagt gctttatttg aggctgtgga acgatttagc
     5881 agcttatatc aaggtgatga ggctattgtc aaagggtgtt tacatgatat ggggccgagc
     5941 gcttttcatc cgaatgattt acaattattc agtgatgcac aattagattc ttggactcag
     6001 gagcatcttc aaaaattagg tgcattaaaa gtcgcaaaac gttataatga agaagatcct
     6061 ttgcactgga ctccaggatg gtctctgact tataattgca gacgttttat tccttttact
     6121 tattgtttct cagatgtgtc tagtcatatc aattcaagtt atgcttgtta cacaggaaat
     6181 ggtgttgctg cgggagtttg tttagaggag gctattttac aggggctttt tgaattagta
     6241 gaacgagatg ctgttgctat ctggtggtac aaccaaattc aaagacccgc tccaaatgca
     6301 gaggttttgg ctgattcctt ttatatcaaa acaaaagaag aatataaatc agcaggctgg
     6361 aatatgtggg tgctagattt aacacatgat ttatcgattc ctgtttatgc tgctcttgct
     6421 caatcggaaa aggacggtcg tttttgtata ggttttggat gtcacctttc ttcaagacta
     6481 gccttatata gggcattaac agagttaaat cagttgtttg atccagcagg agatctgaaa
     6541 accccatggg atgatcatgc gatgacttct agggattact tattcccttc gggagaatcg
     6601 gcatcatcgc gtacttatcc attaaatcaa acaggctttt tggatgatat tcaagaatgc
     6661 cttcatttat taaatcttgc taatctagag ttagttgttg tgaataaaac aaggccagac
     6721 actggagtta aagtggctca tgtcatagtt ccaggtcttc gacatttttg gccaagattt
     6781 ggtaaaggac gtctctatga tgttcctctt aaattaggat ggttaaaaaa gagtccttat
     6841 gaaaaagcat taaatccagt cccgctactt gtttagtata ttattaaaat atgggcaaaa
     6901 ttctaaaaaa tgaaacagaa atatgtcttt ttcgagatca tatttttgga cgcgacttac
     6961 ggtgtagctc tgtattgaaa ggcaatgctg tttcacgttt tcatgcacga attagttgga
     7021 atggagaaaa ttggtgtcta cgtgatctag gaagtcgaaa cggtacttat gtaaatggcc
     7081 gtttgcttgc aacagaaggc gttttttact taaatccagg cgatgtcatt ctttttggtg
     7141 attttgaaga aaagtatctt ttttctgaac gtacgccacc tgaatcttgt ttaatttatg
     7201 ctggaaatga tggcgttgaa aaaaaagtct ctttaactca tttgcttcct ctaccttcca
     7261 tggaacgccc tgtggcaact atttttgctg ggaaaaatgg aggttatttc ttagaaacag
     7321 aatcaggtga attattacct cttcaacatg cagttccttt ttttgtggaa gagataaaat
     7381 acacaccttt ttttgaaaat cctccagatg aatctccaca aacagataca tcggaaatga
     7441 caggtagcag ttccgcttac atcaattttc tagaaatttt agtttctcct gatgaggaaa
     7501 gtgccgaagt gcgttttcat aaaggaaatg aggttttttc tttaaatcat aaggttcatt
     7561 tttatttgct tgcttattta gcgagaaaga gaatcgaaga atcagaagcg caagaaaata
     7621 ctttgaaaga gactaaagac gatgacaatg gttggattga ttgtgaggtc attagtaaag
     7681 aattaatgat gaatcgagat catttaggcc aacaagtctt tagaattcgt caagaattca
     7741 agtccataga ccccttaatt gctgaaaaaa tcatcgatcg tcaacttcga ggaaaaatgc
     7801 gaataggctt tcctgcttca aaaatccaga taaaaaagat gaaataggga ccgtcctatt
     7861 ttgtaatgtt ttgtaatgga ctttttttca tgctagactt aaatttataa atgtaaagaa
     7921 aaattaatgt ttgtaagtta ggttaacgat catggataag aaaagcgcta ttatggctca
     7981 tatgctaaca gaacagggcg gttccgtttt ggtccgctgt gaaggcggct ttatctctcg
     8041 tttcaccctg tcttatgaat ttgaaggtat agaatttagt aaacattctg gtaatatttc
     8101 tttgggcgta aataaatctg aatctgttcc aattggagca aagaatttat ttctaaaagt
     8161 cgaagaaatg tggggttttg gaaggtcaac tatttttatt caacaatttg ctgaacccgt
     8221 tcaaaaatgt tacaaggtat atggtactac attaaatccc aaatggatag aaatctagga
     8281 ggggggagag aaataagcag ccgccaatta atttgagcgg ctgtttattc ttttttaggg
     8341 aggaaagaat aatatgagtt atgatccttt tctaaaaatc tatgtagaac atgaatactc
     8401 tgattttcaa ttatgtccaa cattacgttt acaaccaaca aaaaatacaa cgcattggtt
     8461 aaatcagcat aagtgtattt ctctatatgg cccagggaat gttaagatat caaaacatgt
     8521 ggatatccat gttgagaagc ccgtaatact tgattttcta ggcttttgta gtgatcccaa
     8581 ttttccaaat tacacgttat ttgaaaatga agaagtagaa agtgttcctg tgtatttttg
     8641 taaaacacca agaagcaata atattaaaac tgagtatttg tgggaaaaat gggattatct
     8701 ttttacagaa aataaaataa taaggcccta ttttatcgtt aaagtacaaa tactaccaca
     8761 tgttttacat gaacaaaaaa tatcgattcg cttatcaagt aagaaagttt atgcaaaata
     8821 ttatatcatg ggagagtttg ctcagaaacc aacacaaatc attgatattt cttatgggca
     8881 tgaaaaaatc cattttaatc ctgttcagga aactcttcca gtaagcgctc aatcttttat
     8941 ttctgatcgt ccaattccct tacaaaaagg gaatagaacg cattttcaat tgcaggaaaa
     9001 gggctcttct cgtatattac tttctaggct tccagatgtg aatccaaagt cattaataga
     9061 agcaaaaaaa tcagatggct ctgttatttt aatcgctgaa acattagttc tataatagcc
     9121 tattccaagt aggaccatca aactctttgt cttttctgcg ctctttttgt atcaataata
     9181 agaagcaata gtaagttttg gctaatcggg tagtcaaaat tttgtcataa tacaatttat
     9241 caaaggaatg tgcccgaaag tatggaggtc tcatgaaaaa actatttatc ttcgctgctt
     9301 gtacattatt gattgcctgt gcaagctcat ctaaaaaacc tctgcaatat tcaagtaatg
     9361 atttatttat gcaggtgcaa caagcacaag actctcttaa tagaatagaa aacaactaaa
     9421 aaaaaggctc ttaaataagg gccttctttt tttggataag aagattaatg gtatggttca
     9481 gttttagtgt atttgtagtt ttcgaata
//
LOCUS       DAOOBB010000064         9400 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_9089_length_9400_cov_4.731300, whole genome shotgun sequence.
ACCESSION   DAOOBB010000064 DAOOBB010000000
VERSION     DAOOBB010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 9400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9400
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9089_length_9400_cov_4.731300"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..1536)
                     /locus_tag="PLT31_04365"
     CDS             complement(214..1536)
                     /locus_tag="PLT31_04365"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HPW94405.1"
                     /translation="MKKIFILSLLGLFITSYAQITPTRVGPVSQYGELITGKNSSGKG
                     QIYGSCDSVANGKEVQVRGMSLYWSLLDNALDFWSEEGVTSMVNTMKIQIVRAAMATG
                     TEDWSDGKYKGYTADPNTQKNYVKKVVEAAIKNDIYVIIDWHSHNAHNQTELAKSFFS
                     EMAELYGEYDNVIFELYNEPEKITWSQIKDYANQVIATIRNHSDNLIIVGTPEWDQKT
                     NAAVNNEVTDPENNVAYAFHYYAGSHCFSGQTFWGANCEGQNAVDAMNAGLSVFVSEW
                     GTTDAGGGGDPMGDNTGWQNWMNTHKLSWANWSGSKIPEGSAAFSSGASPTNLQFSTS
                     GNMVKGYLSSNPSSYTTCPDKVSTEAKLPSSEMKITAVSEGIRLSTNQSGLVEIDVFD
                     MLGQSVFSAKEYYNQGSYILPLEKINAGNYLVKAKIGNKTKTITFQKR"
     gene            1683..3251
                     /locus_tag="PLT31_04370"
     CDS             1683..3251
                     /locus_tag="PLT31_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94406.1"
                     /translation="MIRLACFLLLVGSLWAQEAPTGIPVDPSTEEQQALKRISGQIQG
                     KIVWSTSRANSKHDIWIMNADGTGAKALTKSNQVDWFPRFSPDGSKVIFNRSKSGWVP
                     ENDANYPEKWDIWQIGSDGTDEQKIISNATWATYRPDGKAIIFSRAAQVFSFDIATKK
                     ETLLIDGEVIFNKKGVILQEPNMSPDGKHIAITLRGSMRETGVWDLEKKVWTKSGDGC
                     QIDWFADGSRLYRVNPTGNGGTAAPSEILWFTVKDGKPVEKIGFFGIPQAVRLMDLPG
                     RRSHEYFPRVSPDGKWLVWGATDKGHDHDIYDYELYIWKIGSPVNEVTRITYHTGNDR
                     WPDIWLGTLKAKKEEPKKDLSKTEVAKSEAAPARNIEVQVKEFRIKNGQSIFVEESYE
                     KGSSLRSLRVYTRRAKLDVSIALESDKVSNILLEDLTGDGLEDLVLITESAAEGGFKD
                     VYAFVPQEKGPLAVVPLFSWDNSTLPSDYKYKGNDQFNVENGLLVRTLPGLAPLKYRL
                     QLKENGFQFTQEQK"
     gene            complement(3256..3675)
                     /locus_tag="PLT31_04375"
     CDS             complement(3256..3675)
                     /locus_tag="PLT31_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94407.1"
                     /translation="MKSIILNLLFAVGIIFILSCSEEKITAGCNCMSIALEVNAIGKD
                     STTISLDSIQYIYENGEIETIVLSSEDNFDNIVGFQAGEYQVWAFYNGLQSETVTVDV
                     EMAGPEDCRRPSTKRITFQFEDEYKGSLLEKIGNCSE"
     gene            complement(3805..5796)
                     /gene="ligA"
                     /locus_tag="PLT31_04380"
     CDS             complement(3805..5796)
                     /gene="ligA"
                     /locus_tag="PLT31_04380"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPW94408.1"
                     /translation="MNSSSNDFLRYQELCQKLNEASKLYYENAFSPMSDEEFDMGMKE
                     LEALENQHPEWKTASSPVHKVGSDLTNDFPKVTHQVPMLSIANAYNAEEMGDFIRSTT
                     QNSDTQIEWVCERKIDGVSLSVIYENGVLKQAVTRGNGVQGDEVTLNALTINDIPKQL
                     KGAPQGILEVRGEVYMEREAFLALNERFYTEGKKTFQNPRNTVAGSLKLKDPKECAAR
                     PLRYIAYFITQNIGTQTHSQNLEQLRKYGFISNDYWLSNNSEGVMKIADQIYSGRESL
                     AFDIDGMVVKVNSLRIQEELGSTAKSPRWVIAYKFKAERESTQVLSVEFQVGRTGAIT
                     PVANLKPVWLGGTTVKRATLHNFDEIGRLDLHIGDFVLVEKSGEIIPKIIQVEKEKRL
                     PTIETIEKPTHCPVCASPLRQDEEEVVIRCENLHCPAMKQSLLEHFVSREAMNIDNLG
                     PSLIAQLLASGKVKNIPDLYRLTKEDLLELERMADKSADNVITAIAKSKENSLEHFLH
                     GLGIRFIGRTSARNIARHFKNLDDIIQASIEDLKTVPDVGERMAQSLYQFFHQERELA
                     EVLELKALGLPTEFKGSVKNLFQGQTIVITGTLPTLGRNEARQMIEEYGGKVSSSVSK
                     KTSWVLFGADAGSKLTKAEELNIPLHDEAWLLEAIRKEG"
     gene            complement(5885..6433)
                     /locus_tag="PLT31_04385"
     CDS             complement(5885..6433)
                     /locus_tag="PLT31_04385"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HPW94409.1"
                     /translation="MNFLALLWISISLGMDCLAVSISTGLCHCSLAKRKIFGMAFSFG
                     LFQGGMTLLGSLIGNVAQNSFSSISIYIACAILVLLGLRMLYNALFSDPEETSFANVS
                     IRRLIVLSFATSIDAFAVGISLAIIKTPIIKAALIIGLTSFIMSILGAIIGCYSGKKL
                     KSRIPEVLGGLILIFIGIKMLF"
     gene            6621..6986
                     /locus_tag="PLT31_04390"
     CDS             6621..6986
                     /locus_tag="PLT31_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HPW94410.1"
                     /translation="MRSRFFFFIFLFICSLFAAEAIILPKISENVKKEGKLIYNKDCY
                     ACHRWTRDFAGPRMDENIQKYVDQKEELVRYLMNPTPKQPEKYPPMTLEPLSQQDAQV
                     ISSWLFYLLDNPKDPERPR"
     gene            7073..7495
                     /locus_tag="PLT31_04395"
     CDS             7073..7495
                     /locus_tag="PLT31_04395"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HPW94411.1"
                     /translation="MKKTSIPFLHSLHGDSIGLSCEACHSGAQTGREAFLPSKADCMD
                     CHRLPLTESPGIAVLDSILESSPDFPWKRKSRLPSHVVFHHGVHAKASVSCQECHSGE
                     GAWDRGEAPLMLMNDCLSCHRGDRGFAPSATDCASCHR"
     gene            7499..8368
                     /locus_tag="PLT31_04400"
     CDS             7499..8368
                     /locus_tag="PLT31_04400"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPW94412.1"
                     /translation="MLISKRTFIKSFAALLGGFLFFGCRNQGKKSRSRVRSVSVPEIK
                     GEIKKAMQNQKPLDSLVPIKTPGPLKAFPSEINRFGMAIDLDLCDGCGECILACNLEN
                     NIPLVNEYQASKNNYMHWMEMRDQIPVMCFHCGDAPCEKVCPTGAATHSPDGVSAMVF
                     ARCAGTRFCGVNCPIKIRKFNYVDALEEGLQYIANPEVPIRERGVIEKCSLCLHRIQN
                     ARLKAKTLGVEFRGESLTTACAEACSKGAIQFGNWLDSNSKLYKKAKGRGIYTLASMA
                     PFDPSVVYLKGVR"
     gene            8368..>9400
                     /gene="nrfD"
                     /locus_tag="PLT31_04405"
     CDS             8368..>9400
                     /gene="nrfD"
                     /locus_tag="PLT31_04405"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HPW94413.1"
                     /translation="MVKTLFIIGLVLLIPGSSTLFQALSVGPASWALNQTLFWGTPIV
                     HFVFWIGLAHAGTFLSAILLIFEVRFQKRISLVAELSTIAAILVAALFPLIHLGIVSR
                     FYFLVPFFNERDMFVNFNSPLIWDFLAIGAYACLSIVFFISHILSLKIPSFLKTRKVL
                     AWILFPLVLWVHTIVSLDFAVTTDSLWQGAYFPIYFIIGAIFSGLALVLFLLEIMQRR
                     VRKIEEILVAGSWFLLVFWIWEFIQKGVMHSFVLLFGFVFVQLFWLPRFRNTKAVRLF
                     VSFSVLVAMWIERYVLIVHTNIEWLLVDWGWFALGIGLFLSVFFGGVLLFQKYCPQIL
                     LSVPEETSEK"
BASE COUNT         2631 a         1718 c         1914 g         3137 t
ORIGIN      
        1 gatctgcaat taaaattcaa gtaatagctt tgcgaaatga ctgaagcggc atatttttgg
       61 ggaatagagt ctagtgttat gcgtgtgtga aaaattaatt cttttttatg atgaaataag
      121 aattatgttg tgatcattct ttttttgatt ttcatataag taaaaagcca ccagaaaatc
      181 tgatggctct ttttttctct caattgtttt taattatcgt ttttgaaaag tgatggtttt
      241 cgttttgtta ccaatttttg cttttacgag ataatttcca gcattgatct tttcaagagg
      301 gagtatgtag gaaccttgat tatagtattc ttttgcagag aatacgcttt ggcctaacat
      361 atcaaataca tcaatttcaa caagtccact ctgattggtt gaaagacgaa ttccttctga
      421 aacagcggtt attttcattt cagaagatgg aagtttagct tcggtactca ccttatctgg
      481 gcaggtggtg taagaagacg gattgctgct taagtaaccc ttaaccatgt ttcctgaggt
      541 tgaaaattga agattggtag gggatgctcc agaggaaaaa gcggcagaac cttcaggaat
      601 tttggatcca gaccagttag cccatgataa tttatgggtg ttcatccaat tttgccagcc
      661 agtattatca cccattgggt ctccaccccc gccagcatct gttgtacccc attcgctaac
      721 aaatacggaa aggcctgcat tcatggcatc tactgcattt tggccttcgc aattggcgcc
      781 ccaaaaggtt tgtcctgaaa agcaatggct gccagcatag taatggaatg cataggccac
      841 attattttct gggtctgtga cttcgttatt cacagcggca ttggtttttt gatcccattc
      901 aggtgttcct acaatgatta aattatcaga atgatttcta atagttgcaa tcacctgatt
      961 ggcataatct ttgatttgag accatgtaat cttttcaggt tcgttataga gttcaaaaat
     1021 tacgttatca tattcgccat atagttcagc catttctgaa aagaagcttt tggctaattc
     1081 ggtttgatta tgagcattgt gagaatgcca atcaataatg acatagatgt cgtttttaat
     1141 ggcagcttct actacctttt tgacatagtt tttttgagta tttgggtctg cagtatagcc
     1201 tttatattta ccatcgctcc agtcttctgt tcctgtggcc atggctgctc taactatttg
     1261 aattttcatg gtgttcacca tggatgtaac gccttcttcg gaccaaaaat cgagagcatt
     1321 gtctaaaaga ctccaataaa gactcattcc cctgacttga acctcttttc cgttggcaac
     1381 actatcacaa gagccataga tttggccttt gccagaagaa tttttaccag taattagctc
     1441 tccgtattgg ctaacagggc caactcttgt gggtgtaatt tgagcataag aagttataaa
     1501 tagtcctagt aaggaaagaa tgaatatttt tttcataaga gcctcgtctc taaatcaatc
     1561 tatcctaaac ataaattgaa aaaataaaat ggagctaata acagttattt ttattgtata
     1621 cagatgtgta atgttatctt aggtcaacat ttttactaaa ttagacgtac aatggagatt
     1681 ttatgattag attggcttgt tttttgttat tagtgggctc tttatgggct caagaggctc
     1741 ccacaggtat tccagttgat ccttctacag aagaacaaca ggccttaaaa cgcatttctg
     1801 ggcaaattca agggaaaata gtctggtcta cgagtcgtgc gaattcaaaa catgacattt
     1861 ggattatgaa cgcggacggc acaggggcaa aagctttaac aaaaagcaat caagtggatt
     1921 ggttcccacg cttttctcca gatggctcta aagtgatttt taaccgctct aaaagcggtt
     1981 gggttcctga aaatgacgcc aattatccag aaaaatggga tatttggcag attggttccg
     2041 atggcacaga cgaacaaaaa atcatttcga atgccacatg ggctacatat agaccagatg
     2101 gtaaggctat tattttttca agagccgctc aagtcttttc ttttgatatt gccacaaaaa
     2161 aggaaacttt actcattgat ggggaagtga tttttaacaa aaaaggcgta atcttacaag
     2221 aacctaatat gtctcccgat ggtaaacaca ttgcgattac acttcgcggc tctatgagag
     2281 aaacaggcgt ttgggattta gagaaaaaag tttggacaaa atcaggagat ggctgccaga
     2341 ttgattggtt tgccgatggc tctagattat atcgtgtgaa tcctacaggt aatggaggca
     2401 ccgctgcccc tagtgaaatt ctttggttta cagtgaaaga cggaaagcct gtcgagaaaa
     2461 ttggcttctt tggtattcct caggcggttc gtttgatgga tcttcctggc cgtcgttcgc
     2521 atgagtattt cccaagagtc tctcctgatg gcaagtggct cgtttggggg gctaccgata
     2581 aaggccatga tcatgatatt tatgattatg aattatacat ttggaaaatt ggttctccag
     2641 tcaatgaagt aactcgaatc acttaccata caggtaatga ccgctggcca gatatttggt
     2701 tagggacttt aaaggctaaa aaagaagaac cgaaaaaaga tctttctaaa acagaggtag
     2761 ctaaatcaga agccgctcct gctagaaata tagaagtgca agttaaagaa ttccgcatta
     2821 aaaacgggca aagcattttt gttgaagaat cctatgaaaa aggatcttca ttgcgttccc
     2881 ttcgcgttta tactcgtcgt gctaaattag acgtttctat agccttagaa agcgataaag
     2941 tctcgaatat tcttttagaa gacttaacag gagatggttt agaagacttg gtcttaatca
     3001 ctgaaagtgc agcagaaggt ggctttaagg atgtgtatgc gtttgtgcct caagaaaagg
     3061 gaccacttgc cgtagttcca cttttctctt gggataattc aacgcttcct tcagattata
     3121 aatacaaagg aaatgatcaa tttaacgtag agaatggcct attagttcgc acacttccag
     3181 gcttagctcc attaaagtat cgcttgcagc taaaagaaaa tggctttcag ttcactcaag
     3241 aacagaagta actttctatt cgctacagtt gcctattttt tctaataggc tccctttata
     3301 ttcgtcttca aattgaaacg taattctttt tgtggatggc cgcctgcagt cttctgggcc
     3361 tgccatctct acatctacag taaccgtctc tgattgtaag ccattataaa aagcccaaac
     3421 ttggtattcg ccagcttgaa aacctacaat gttatcaaaa ttatcctctg aagataaaac
     3481 aatcgtttcg atttcaccat tttcgtagat gtattggatt gaatccaatg aaatagtagt
     3541 actatctttt ccaatagcat ttacttctaa tgctatggac atgcaattac atcctgcggt
     3601 tattttttct tctgaacatg aaagaatgaa aatgattcct actgcaaata ataagttcaa
     3661 gataatgctc ttcatatgga ctccttaaaa ttactccttc aacaatttac ttttattact
     3721 ccatgtagcg gcatccattt gatttttttt gtcactatat acaagatttt acgcttttaa
     3781 aatcttgata aaacagtaat tcaatcaacc ttcttttcta atcgcttcta gtagccaggc
     3841 ttcgtcatga agaggaatat ttaattcttc ggctttggtg agtttgctgc cagcatctgc
     3901 accaaagaga acccaacttg tttttttact gaccgaggag gaaactttgc ctccgtattc
     3961 ttcgatcatt tggcgggctt cgtttcggcc tagggtaggt aaagtgcccg tgatgacaat
     4021 cgtttggcct tggaatagat ttttgacaga ccctttgaac tctgttggta atccaagagc
     4081 tttgagttct aagacttcgg caagttcacg ttcttgatga aaaaactggt agaggctttg
     4141 agccatgcgt tcgccgacat caggtactgt ttttaggtct tcgatggagg cttgaataat
     4201 atcatctagg tttttaaagt gtctcgcgat gtttcgagca cttgttctgc caataaaacg
     4261 aatgcctaaa ccatgtaaaa aatgttctaa gctgttttct ttgctttttg caatcgctgt
     4321 gatcacgtta tcggcacttt tgtcggccat gcgttctaat tcaagaaggt cttctttggt
     4381 taagcgatag agatctggaa tgtttttgac ttttccagag gcgagaagtt gagcgattaa
     4441 acttggccca agattatcaa tattcatggc ttctcggctt acaaaatgct cgagaaggct
     4501 ttgtttcatc gcaggacagt gtaagttttc gcagcgaatc accacttctt cttcgtcttg
     4561 tcgcaaagga gaagcacata ctgggcaatg tgttggtttt tctatcgttt caatagtagg
     4621 aagccgtttt tctttttcga cttgaatgat ttttggaata atctctccgc ttttttcgac
     4681 taaaacgaag tctccaatgt gaaggtctaa gcgcccgatt tcatcaaagt tgtgtagtgt
     4741 ggctcgtttg actgtagtgc cgcctaacca aacaggtttt aagttcgcaa cgggtgtgat
     4801 tgcccctgtt cggccgactt gaaattcaac agaaaggact tgagtcgatt cgcgctcggc
     4861 tttaaactta taagcaataa cccagcgagg gcttttcgct gtagagccta gttcttcttg
     4921 tatgcgtaaa gaattgactt tgacgaccat gccatcgata tcaaaggcca gtgattctct
     4981 gccagaataa atctgatctg ctattttcat cacgccctct gaattattag atagccaata
     5041 atcgttactt ataaacccat attttctgag ttgttctaag ttttgagagt gtgtttgtgt
     5101 gcctatattt tgcgtaataa aataggcgat atagcgaagc ggccttgcgg cacattcttt
     5161 aggatcttta agtttaaggc tcccagcaac agtatttctc ggattttgaa acgttttttt
     5221 gccttcggtg tagaatcgtt cgtttaacgc gagaaacgct tcacgttcca tatagacttc
     5281 gcctcggact tctaatatgc cttgaggtgc gccttttaat tgtttgggaa tatcatttat
     5341 agtcagggca tttagggtca cttcatcgcc ttgaacgcca ttgccgcgag tgactgcttg
     5401 ttttaaaacg ccgttttcat aaatgacaga gaggctcacg ccgtcaattt tcctttcaca
     5461 gacccattct atttgagtgt cgctattttg tgtggtgctt cgaataaaat cacccatctc
     5521 ttctgcattg tacgcatttg cgatgctcaa catgggcact tgatgagtga cttttggaaa
     5581 atcatttgtt aaatcactgc cgactttgtg tactggagat gatgctgttt tccattcagg
     5641 gtgctggttt tcaagagctt ctagctcctt cattcccata tcgaactctt catcactcat
     5701 aggtgaaaag gcattttcat agtagagttt gcttgcctca tttaattttt ggcatagctc
     5761 ttggtatcta agaaaatcat ttgaagaaga gttcataaaa agcctttaaa aacggttggg
     5821 gctacttttg aatgttttta taaaaataat ttttgataga attcataaac tatttataac
     5881 atttttaaaa tagcatttta atgccaataa aaattaaaat aaggccacct aaaacttcag
     5941 ggattcgact ctttaatttt ttaccagagt aacaaccaat tatggcgcct aaaatgctca
     6001 ttataaatga tgtgagtcca atgatcaatg ccgctttaat aattggggtt tttatgattg
     6061 ctaaagaaat tccaacggca aaagcatcaa tacttgtggc aaaagaaagg actatcaaac
     6121 ggcgaatgga aacattagca aaagatgttt cttcaggatc tgaaaaaaga gcgttataaa
     6181 gcattctaag gccaagtaaa actaaaatgg cacaggcgat atagatgctt atagagctga
     6241 aagagttctg agcgacgttt ccaattaggc ttcctaaaag agtcattcca ccttgaaaaa
     6301 gcccaaagga aaaagccata ccgaatattt ttcttttggc taaggagcaa tggcaaaggc
     6361 ctgtagaaat agaaacggct aagcaatcca ttcctaggct aattgaaatc cataaaagag
     6421 caagaaaatt catgaaaaat ccgtagctaa aaacagcaag aatatagaaa aatacgatta
     6481 gattatatca gaatgattga ttcttacaaa tatcatggaa caaaaccaat actaaaaagg
     6541 gagcgattgg atacattctg aactttttag gatttaatct tttatatttc ttaaagaaag
     6601 agttatataa aggtgtgtat atgaggagtc gctttttttt ctttattttc ttgtttattt
     6661 gctcgctttt tgcggcagag gcaattattt tgcctaaaat ttcagaaaat gtaaaaaaag
     6721 aaggaaaact gatttataat aaggattgtt atgcatgcca tcgttggact cgagattttg
     6781 cagggcctcg tatggatgaa aatattcaga agtatgtgga tcaaaaggag gaactggttc
     6841 gctatttaat gaatcccact cccaaacaac cagaaaaata ccctccaatg actttagaac
     6901 ctctttcaca acaagatgcc caggttattt cttcttggtt attttacctt ttagataatc
     6961 ctaaagatcc cgaaaggcct cgatgaaaaa aggtatttta ttgctcctat ttctcttttt
     7021 ttcagcttta attttagctg attttttctt ttcgccgtct gtgtctccta atatgaaaaa
     7081 gacttcaatt ccttttctac attctctaca tggagattcc attgggcttt cgtgtgaagc
     7141 gtgccattca ggggcgcaga cgggtagaga ggctttttta ccttctaagg ccgattgtat
     7201 ggattgccat cggttgccat taacagaatc tcctggaatt gcggttttag atagtattct
     7261 agaaagctcg cctgattttc catggaaaag gaaaagtcgt ttgccttccc atgttgtttt
     7321 tcatcatggc gtgcatgcca aagcgagtgt ctcttgtcag gaatgccatt ctggtgaagg
     7381 tgcttgggat cgaggagagg ctccgctcat gttgatgaat gattgtcttt cttgtcatcg
     7441 tggagatcgt gggtttgccc cttcggccac cgattgtgct tcttgtcata ggtaatttat
     7501 gttgatttct aaacgaactt ttattaaatc atttgctgct ttgctaggtg gatttctttt
     7561 ttttggatgc aggaatcaag ggaaaaaaag ccgttctaga gttcgtagcg tttctgttcc
     7621 agaaatcaag ggtgaaatta aaaaggcgat gcaaaatcag aagcctttag attctcttgt
     7681 tcctatcaaa acacctggtc cattaaaagc gttccctagt gaaataaacc gttttgggat
     7741 ggcaatcgat ttagatttat gtgatggttg tggcgaatgc attttagctt gtaatttgga
     7801 aaataatatt ccgttggtga atgaatatca agcgtctaaa aataattata tgcactggat
     7861 ggagatgcgc gatcaaattc cagtgatgtg ttttcattgt ggagacgctc catgtgaaaa
     7921 agtttgccct acgggagcgg cgacacattc tcctgatggg gtaagcgcga tggtctttgc
     7981 taggtgtgcg ggcactcgct tttgtggcgt taattgccct attaaaatca gaaaatttaa
     8041 ttatgtagat gcgttagaag agggactaca gtatattgcc aatccagaag tgcctataag
     8101 agaacgtgga gtaatagaaa aatgctctct ttgtttacat cgaatccaaa atgctcgact
     8161 caaagctaaa actcttggag ttgagtttcg tggagagtct ttgactacgg cttgtgcaga
     8221 agcgtgttct aagggagcaa ttcaatttgg aaattggctt gattctaatt cgaagcttta
     8281 taaaaaagcg aaaggaagag gcatttatac tcttgcgtcc atggctcctt ttgacccttc
     8341 agtcgtttac ctcaaagggg tgcgctgatg gtcaaaacgc tttttattat tgggctagtt
     8401 ttattgattc caggtagctc tactctcttt caggctctaa gtgtaggccc cgcttcttgg
     8461 gctttaaatc aaacgctttt ttggggaacc cctattgttc atttcgtctt ttggataggc
     8521 ttagctcatg cgggtacttt tttatcggca atacttttaa tttttgaggt ccgatttcaa
     8581 aaaagaatat ctctcgttgc cgaactttcg acgattgctg cgatacttgt tgccgctttg
     8641 ttccctttga ttcatttagg gattgtctct cgcttttatt ttttagtccc tttctttaat
     8701 gaaagagata tgtttgtgaa ttttaattct ccgctaatat gggatttttt agccatcgga
     8761 gcttatgctt gcttatcaat tgtttttttt atttctcata tactttccct aaaaattccg
     8821 tctttcttaa aaaccagaaa agtcttagct tggatcttat tccctttggt gttatgggtg
     8881 catacgattg tgagtcttga ttttgcggta acaacggact ctttatggca aggagcttat
     8941 ttcccgattt acttcattat tggagccata ttctctgggt tagctcttgt cctattttta
     9001 ttagaaataa tgcaaaggcg agtgcgtaaa attgaagaaa tacttgttgc tggatcttgg
     9061 tttttactcg ttttttggat ttgggaattt attcaaaaag gcgtaatgca ttcatttgtt
     9121 ttactttttg gttttgtttt tgtgcaatta ttttggctcc cgcgctttcg aaatacaaaa
     9181 gcagtccgtc ttttcgtttc attttcggta ttagtcgcta tgtggattga acgatacgtt
     9241 ttaattgtcc atacgaatat agaatggctt ttagtcgatt ggggatggtt tgctttagga
     9301 ataggtttgt ttttatctgt attctttgga ggtgtattat tatttcaaaa atattgtcct
     9361 caaattttac tatcagtacc agaagaaact tctgaaaagt
//
LOCUS       DAOOBB010000065         9379 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_9106_length_9379_cov_4.403797, whole genome shotgun sequence.
ACCESSION   DAOOBB010000065 DAOOBB010000000
VERSION     DAOOBB010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 9379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9379
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9106_length_9379_cov_4.403797"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1060
                     /locus_tag="PLT31_04410"
     CDS             149..1060
                     /locus_tag="PLT31_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW94414.1"
                     /translation="MIQIEGLNKTYHTGFLMRPKKALFDIHLNVPKGIIYGFIGPNGA
                     GKSTTIKVLTGLLNYDSGKVQVAGYSPRVVKSRTHLGYCPEQPYFYDYLTGRELMSFY
                     GRLAGLQGVELQKRIEWALNLVHANKDWIDRRLRTYSKGMMQRVGLAQSILSKPELLI
                     LDEPMSGLDPMGRRDVREAILSLNQEGVTIFYSSHLLSDVESISDRVAMIVDGKIVRE
                     GSIQEVTSSCSVQYHVRTGEITSNIDLPKGVKASTQSGEWTCEDAVARDLFIQFCLNN
                     GISIERIDHHRPSLEDILTEEIARAES"
     gene            1047..1850
                     /locus_tag="PLT31_04415"
     CDS             1047..1850
                     /locus_tag="PLT31_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW94415.1"
                     /translation="MQSLSHIGIIALNTFRESIRDKILYNIVFLAIGLALFSILLGEW
                     SVFDRAHVIKSTTLTIMSLSGLLISVFVGISLVQKEIQRKTVLTLLSKPISRVSFIVG
                     KYLGLLGVVASHLFLLTIVYYLILFLTHSSPSLSLLSAIYLVFCEMAVVVAVALLFSS
                     FSTPVLSALFTLGVYFAGHLSNQLLEQVQFVKRMGEVEGFSSILMEKMAIVIHAVFPG
                     LYRYNISSYVVHQIALPEWYLFWQSLYAIGYIGVFLAVASWWFNRRDFL"
     gene            1847..3472
                     /locus_tag="PLT31_04420"
     CDS             1847..3472
                     /locus_tag="PLT31_04420"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPW94416.1"
                     /translation="MRNQYMAKVLFSNNLVTNEQVQEYWGKITRDKDIGQVLCEAGLL
                     DKNMYEKVLAYVQSLEAKLSSESKPTPPSTAQSTPSAAPSEPKKVPPQVKPQKEIRPP
                     TNSKKLDLPEEPSFKIEGNSLYGDASTTPIQVEVISGLESTSITNIQLKAEEDQIEIE
                     GITALPDQFAIETGEGEIAVPDAIDPSMSLTQILSYARKFNSTDIYLYDNRPIVIRQS
                     GSLHYACETVIESNQLIQWLAEASRGFVDGKEPAVGKNFSKTFALTGAGRARLSVTWV
                     DVVPFLAIRIIPLESVAFDDLYLPAFCKDFLELESGLILIAGPSSSGRSTTMSTFAET
                     IAKNNWVYIQTIEKPIERLLSNPNGIIAQKEVGLHTSSGLQGIRSAINDGADIILFDH
                     LEKMDELGLLLQAANAGTLVFAVASGNNILALLTRFLDSVSDIQRPVLAQSLADQLKG
                     VIVQHLVPVVDKQGEVLAVEAMKTSPSIANMIRKGELSQIPAALSAMKGSGQTLDDSL
                     QKLVESGYIEGIEAWKRAFDTRRFASYRPSKKN"
     gene            3487..4491
                     /locus_tag="PLT31_04425"
     CDS             3487..4491
                     /locus_tag="PLT31_04425"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPW94417.1"
                     /translation="MSSPIEDCLEYALNARASELIIVEGLSPAVRLNDKVCMIQDAPV
                     IPFGSLDSFVKSLDGESGVLCGGPWCGANWRVRYSREAFGKLASFRPMMPECPHFSEL
                     GVPDVMMNLLGVSSGLVLFAGPPCSGKTTTASSYVAAMCQNRMLRGVFLDSVEEFNIP
                     TGDSLVIKSKSKDITKELKQGLLAGTDLFWLGDIDDEHIIPALRAAEAGAIVVGCFNA
                     GNSVGALDAMVCFESSKEMKIAKSLLASNLKALVIQHLIPSVDNSQMVSAWELIYNNQ
                     NISNLIRNGEHFKLPPIVAASTTEGMVSMDHSLLALLQAGVISKKDALKYAFDPSKIG
                     "
     gene            4517..6652
                     /locus_tag="PLT31_04430"
     CDS             4517..6652
                     /locus_tag="PLT31_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94418.1"
                     /translation="MIIWHGSFLWASPLTPGELKKTVLYLGGGDQSPWYFLGVFYAID
                     AYKIPVDSIVASSWGAWVSALWMEGFSPDAIQKLFLDPEVKPFVGQEQLSLNRFSKKE
                     PLLPIGNGNIPSLQMRFFIESDTAGFLKMVKRPLNSDSLTIKNALFHFRIQESLSQKM
                     IPPRKPFSALSCDGTSGKSMSDIFKTLPLVGHHASGELCHLIPLPSKNDSSLISIVAV
                     PVPLRNKDTIAEPWKRELWHRALQGLKNSPSPIVILRPHQMTQNTPEAWIQAGFSAVE
                     SRLGDLSKVLGRKPSSIRSLDSLVPWFRYNPSFEKVPSEIYSHIKSYWNPADTGIKGP
                     EHFVSEIMSNPLYDSLSFELQDNGDLLIGASAPTLIDFKLGGFGSNIWGPNAYSKIDF
                     RFINQFEYLFSIEAFYGLHSYGIRPELRLSRLWQGRGDFFVNADFSHREVLKKYFSEE
                     YDWNRIFSESRTDLNLGFSYEVLPQHQLDFNVLFGSRTLETKYSKEEGDFESTPIEPG
                     VTYKYSTPDYKSWFGYSGFSVEGSLSMRSIHAGVYGFIPMHYRSKVDLQYYYSPTTFL
                     TLGAGAAGAISMYHKEGYGYVYPELLEFEPLDNSYRHAMSPTPWSTEWYFAEMLSYHY
                     GLIRLQAGLHNNWLGLWIFGSYIKDFEENPTVLIKDHRFILEPTLRFAYRSFDVRAGL
                     SRMVDFEHLKEFKDFKNYHFFVKFGAYQF"
     gene            complement(6715..7725)
                     /gene="mltG"
                     /locus_tag="PLT31_04435"
     CDS             complement(6715..7725)
                     /gene="mltG"
                     /locus_tag="PLT31_04435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPW94419.1"
                     /translation="MKKFFIFIFFVLLATCLTGVFFVKKHIETPSSNAKAVILEIPKG
                     SSAAKVSQILQNSGIWESPESFQLWCRIKKCALKAGWFEIPPHQKIRDLVAIFESGKN
                     AVRKVTIPEGRASWEMPQYLKKEFPELAVEVWDSLVHSSEFTRSLGVNASNLEGYLLP
                     ETYMFPYQSDEQTILKTMVSANLRLQEEMKSQGSEVWKKLGGWHQILTLASVVEEETG
                     NPSERPLIAGVFHNRLDIGMPLGADPTVRFIFKNLTGPIYKSQLNSDSPYNTRKFKGL
                     MPGPISNPGRHAIEAVLKPEVTDKLYFVAKDDGSGTHFFSSTLSKHNYYKEIAAQNRL
                     HH"
     gene            7825..8262
                     /locus_tag="PLT31_04440"
     CDS             7825..8262
                     /locus_tag="PLT31_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94420.1"
                     /translation="MKKILFCVLALMVAVSFAAPKKVKSKRGDVELTKDKSGGAVVCT
                     AGFNDEMTIVKDGDTEVLVKASCGQGWVQKSQVEYIVAAAGDKSMKLDDVDVIGWLDN
                     PSAVFVLEQDAADFEGVNIDRDFKEYLQHTMDREQVEMKNNEN"
     gene            8889..>9379
                     /locus_tag="PLT31_04445"
     CDS             8889..>9379
                     /locus_tag="PLT31_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94421.1"
                     /translation="MAKQKIDSLSYWSDLSETKTALGSQVNLKQEIVKNPKEVLKNLN
                     KDVVIATNKKNEDLTIGDVLAKLNEKNRSAIVSVFVDSAIQIEKAQKKFITELTAVLP
                     KDLATKEDFFLVKPQIPIKTQIPIKTQIPIKTQIPIKTQIPIKTQIPIKTQIPIKTQI
                     PIKT"
BASE COUNT         2833 a         1618 c         1886 g         3042 t
ORIGIN      
        1 aacttgtttt acaatttatt cccaggtctt gaaggcttgt ctttgaaccc tcttttttat
       61 ttgggtatag ctttttatat cttgggagct tcactttact atttagtgaa taaaaaagaa
      121 cgtcaaataa aaaagaatga ggatgaggat gattcaaatt gaagggttga ataaaactta
      181 tcatactggt tttttgatga ggcctaaaaa ggcgttattc gatattcatt tgaatgtgcc
      241 aaaaggaatc atctatggtt ttattgggcc taatggagca ggcaaatcga cgacgataaa
      301 agtcttaaca gggctcttaa attatgattc tggaaaagtc caagtagcag gttattctcc
      361 tcgagttgta aaaagcagaa ctcacttagg gtattgtcca gaacaacctt atttttatga
      421 ttatttaact ggtcgagaac tgatgtcttt ttatgggcgt cttgcaggcc tacagggagt
      481 agaacttcag aaacgtatcg aatgggctct aaatcttgta catgcaaata aagattggat
      541 tgacagacgt ttacgtactt attcgaaagg catgatgcag agagtgggtt tagcacaatc
      601 gattttatct aagccagagt tactgattct tgatgaaccg atgagtggtc ttgatcccat
      661 ggggaggcga gatgtacgag aagcgattct ctcattaaat caagaaggcg tgactatttt
      721 ttattccagt catttacttt ctgatgtaga atctattagt gatcgagtcg cgatgatcgt
      781 tgacgggaaa attgtgcgag agggttctat tcaagaggta acatcgtctt gttctgttca
      841 gtatcatgta agaacaggag aaattacttc taatattgat ttacctaaag gagtcaaagc
      901 ctctacgcaa tctggagaat ggacttgtga agatgctgtg gctagagatc tctttattca
      961 gttttgctta aataatggaa tttctataga acgaatagat catcatagac caagtctaga
     1021 agacatctta acggaggaga tagctcgtgc agagtcttag tcatataggc attattgcat
     1081 taaatacttt tagagaatcc attcgcgata agatccttta taatattgtt tttttagcga
     1141 ttggccttgc tctttttagc attcttcttg gagaatggtc tgtttttgat cgagctcatg
     1201 tgatcaaatc tactacactt acaatcatgt ctctttcggg gcttttgatt tctgtatttg
     1261 tggggattag tcttgttcaa aaagagattc aacgcaaaac agttttgact ttactttcaa
     1321 agcccattag tcgagtttct tttattgtag gaaagtattt aggcttatta ggtgttgttg
     1381 ctagtcattt gtttttactc acgattgtct actacctcat tcttttttta acgcattctt
     1441 ctccttcatt atccttacta agtgctattt atctcgtgtt ttgtgaaatg gccgttgtcg
     1501 ttgcagtggc attacttttc tctagcttta gtactcctgt tttaagtgcc ctatttacat
     1561 taggcgttta ttttgcaggg catttatcca atcagttgct cgaacaagta cagtttgtca
     1621 aaagaatggg tgaagtggaa ggctttagta gtatactcat ggaaaaaatg gctattgtta
     1681 ttcatgccgt ttttcctgga ttgtatcgct ataatattag ttcttatgta gtgcatcaaa
     1741 ttgctttgcc tgaatggtat ttgttttggc agtctcttta tgccataggg tatattggtg
     1801 tttttttggc agtggcttct tggtggttta atcggaggga tttcttatga gaaatcaata
     1861 catggcaaaa gttttgtttt caaataactt agtcaccaat gaacaagttc aagaatattg
     1921 gggtaagatc actagggata aagacattgg acaagttctt tgtgaagctg gtttgttaga
     1981 caaaaatatg tatgaaaagg ttttggctta tgtccaatct ctagaggcaa aattgtcttc
     2041 agagtcgaag cctacgcctc catctactgc tcaatcaacg ccttccgcag caccatctga
     2101 acccaaaaag gtgcctccac aggtaaaacc acaaaaagaa attcgtcctc ccacgaattc
     2161 aaaaaaatta gacttgcccg aagaaccttc ttttaaaata gaaggaaaca gcttatacgg
     2221 agatgcttct acgactccta ttcaagtaga agtaatttct ggtcttgaat ctacaagcat
     2281 cacaaatatt cagttaaaag cagaagaaga tcaaatagaa atagaaggca taaccgctct
     2341 gcctgatcag tttgctattg aaacaggtga aggcgaaatt gctgttccag atgccattga
     2401 tccttcaatg tctttaactc agattctttc ttatgcccga aaattcaact cgacagatat
     2461 ttatctttat gacaatcgac caatagtgat tcgtcaatct ggttctttac attatgcttg
     2521 tgaaacagtc attgaatcta atcaactaat acaatggctt gcagaagctt ctcgtggatt
     2581 tgtcgatggg aaagaacctg ctgtgggtaa aaacttcagt aagacttttg ccttaactgg
     2641 tgcagggaga gcacgtcttt cagtgacttg ggtagatgta gtaccgtttc ttgcgattcg
     2701 aatcattcca ttagaaagcg tcgcttttga tgacttgtac ttgcctgcat tttgcaaaga
     2761 ttttttagaa ctagaaagtg gacttatttt aattgctggt ccttcttcta gtggacgctc
     2821 cactaccatg agcacttttg cagaaacgat agccaaaaat aattgggtgt atattcaaac
     2881 tattgaaaag ccaattgaac gattactaag taatcccaat ggaattattg ctcaaaaaga
     2941 agttggatta cacacttctt ctggtttgca aggtatccgt tctgcgatta atgatggtgc
     3001 cgatattatt ctatttgacc atctggaaaa gatggacgaa ttaggtcttt tattacaggc
     3061 cgccaatgcg gggacgcttg tttttgctgt cgcttcaggt aataatattt tagccttatt
     3121 aacacgtttt ttagattcag tttctgatat tcaacgtcct gttttagctc aatcgcttgc
     3181 ggatcaatta aagggcgtga tcgtgcagca tttagtgcct gtggtagata aacagggaga
     3241 agtgcttgct gtagaagcaa tgaaaacatc tcctagcatt gccaatatga ttcgaaaagg
     3301 ggagctttct caaatccctg ccgccttgag tgcgatgaaa ggctcaggcc aaacattaga
     3361 tgattcgtta caaaagcttg tagaatcggg gtatattgaa ggtattgaag cttggaagag
     3421 ggcttttgat actcgtcgtt ttgcatcgta tcgtccgtct aaaaagaatt aaaaggaaaa
     3481 gcagctatga gttcacctat tgaagattgc ttagaatacg ctttaaatgc tcgtgcaagc
     3541 gaattgatta tcgtagaagg attatctcct gctgttcgct taaacgataa agtctgtatg
     3601 attcaagatg ctccagtgat tccttttgga tcgttagatt cttttgtgaa gtcattagat
     3661 ggcgaatctg gagtcttgtg tggcggcccg tggtgcggcg caaattggcg tgttcgttat
     3721 tcaagagaag cctttggaaa attggcaagc tttagaccca tgatgcctga atgtcctcac
     3781 ttcagtgaat taggtgttcc tgatgtcatg atgaatcttt taggagtgag ttcaggatta
     3841 gttcttttcg ctggtccacc ttgctctgga aaaacgacaa cagcttcctc ttatgttgct
     3901 gccatgtgtc agaatcgcat gttacggggt gtatttcttg actcggtaga agagtttaat
     3961 atcccaacgg gagatagtct tgtcatcaaa tccaaaagca aagatattac aaaagaatta
     4021 aaacaaggtc ttttagcagg gacagatcta ttttggcttg gagatataga tgatgagcat
     4081 atcatccctg ctttacgagc tgcagaagca ggtgctattg ttgtagggtg ctttaatgca
     4141 ggtaattctg tgggcgcttt agacgctatg gtttgctttg aatcatctaa ggaaatgaaa
     4201 atagcgaaat cacttttagc ttctaatttg aaagcacttg ttatacaaca tttaattcct
     4261 tcggtggata attctcaaat ggtctctgct tgggaactta tctacaataa tcaaaatatt
     4321 tcaaatctga ttcgaaatgg tgagcatttt aagcttcctc ctattgtagc cgcaagcact
     4381 acagaaggga tggtttctat ggatcattct ttactggctt tacttcaagc tggagtgatt
     4441 tctaaaaaag atgcgttaaa gtatgctttt gatccttcga aaataggatg acatgaaaaa
     4501 gtttttttta attctaatga taatctggca cggttccttt ttatgggcat cgccactgac
     4561 tccaggagag ttaaaaaaaa cagtgcttta tttaggcggg ggtgaccaat cgccgtggta
     4621 ttttctaggt gttttttatg ccattgatgc gtataaaata ccagtggatt ctattgtcgc
     4681 ttcttcttgg ggggcatggg tttctgccct ttggatggag ggcttttctc cagacgccat
     4741 tcagaaatta tttttagacc cagaagtaaa accatttgta gggcaagaac agctttcatt
     4801 aaatcgcttt tcgaaaaaag agcctctatt gcctattggg aatggaaata ttccctcttt
     4861 acaaatgcgc ttttttattg aaagtgatac agctggtttc ttaaaaatgg tcaaacggcc
     4921 cttaaattcg gattctctta cgatcaaaaa tgccctattt catttccgaa tacaagaaag
     4981 tttgtctcaa aagatgatcc cgcctaggaa gcctttttca gcattgtctt gtgatggtac
     5041 atctggaaaa tcaatgagtg atatatttaa aacgctccct ttagttggtc atcatgcatc
     5101 gggagaactt tgtcatctca ttccactacc ttcaaaaaat gatagctcgt taatttctat
     5161 tgtagctgtt ccagttcctt taagaaataa ggatacaatt gctgaacctt ggaaaagaga
     5221 attatggcat agagccttgc aaggcttgaa aaatagtcct tctcccatag taatccttcg
     5281 ccctcatcaa atgacacaaa atactccaga agcatggatt caagcaggtt tttcggctgt
     5341 agaatcgcgc ttaggagatc tttcaaaagt gttaggtcga aagccttctt ctattcgatc
     5401 tttagattct ttagtccctt ggttccgtta taatccatct tttgaaaaag tcccttcaga
     5461 aatatactca cacatcaaat catactggaa tccagcagat acagggatta agggaccaga
     5521 gcattttgtt tctgaaatta tgagcaaccc tttgtatgat agcctttctt ttgaattgca
     5581 agataatgga gatttactga taggggcaag tgcaccgact ttgatcgatt ttaaattagg
     5641 tggttttggg tcgaatattt gggggccaaa cgcttattct aaaatagatt tccgttttat
     5701 aaatcagttc gaataccttt tttctataga ggctttttat ggattacatt cttacggtat
     5761 acgcccagaa ttaaggctct cacgcttgtg gcaagggcgg ggtgattttt ttgtaaacgc
     5821 agatttttca catcgagaag ttctaaaaaa atatttttca gaagagtatg actggaatcg
     5881 aatatttagt gaatcaagaa cggatttaaa tcttggattt tcttatgaag ttttaccaca
     5941 acaccaatta gattttaatg ttttattcgg atctagaact ctagaaacaa agtactctaa
     6001 ggaagaaggg gattttgagt cgactccaat agaaccagga gtcacctata agtatagtac
     6061 tcctgattac aaatcatggt ttggttattc aggcttttct gtagaaggct ctttatctat
     6121 gcgctctata catgcaggag tatatggatt tattcctatg cattatcgct caaaagttga
     6181 ccttcagtac tattattctc caacgacttt tttaacatta ggagcagggg ctgctggagc
     6241 cataagcatg tatcataaag aagggtatgg ctatgtttac ccagaattat tagaatttga
     6301 acccttagat aattcttata ggcatgcgat gtctcctact ccttggagta cggaatggta
     6361 ttttgcagag atgctttctt atcattatgg cttaatccga ttacaagcag gattgcataa
     6421 taattggtta gggttatgga ttttcggttc ttacataaaa gactttgaag aaaatccaac
     6481 cgtattaatc aaagatcatc gttttatttt agaacccacg ctacgattcg cttatcgttc
     6541 atttgatgta cgtgcaggtc ttagtcgaat ggtggatttt gagcatctca aggaattcaa
     6601 ggactttaaa aactaccact tttttgtgaa gtttggtgcc taccaatttt agatagtttt
     6661 tttattaatg attcccttta aaatcattta accaaagtga tttttaaggg agccttaatg
     6721 atgcaagcga ttttgagcgg cgatttcttt atagtaattg tgtttgctta acgtagaact
     6781 aaagaagtgg gttccagaac catcatcttt tgcaacaaag tataatttat ctgtaacttc
     6841 tggttttaaa accgcttcaa tcgcgtgacg cccagggttt gaaataggac caggcattaa
     6901 acccttaaac ttacgagtgt tgtatgggct atcgctatta agctgactct tatagattgg
     6961 acccgttaaa ttcttaaaaa taaaacgcac agtagggtct gctcctaaag gcataccgat
     7021 atctaaccta ttatgaaaaa cacctgcaat tagaggcctt tcagaagggt ttcctgtttc
     7081 ttcttcaacc acgcttgcta gagttaatat ttgatgccag ccgccgagtt tcttccaaac
     7141 ttctgagcct tgagacttca tttcttcttg taatcttaaa ttggcagaaa ccatcgtttt
     7201 aagaatcgtt tgttcgtcgc tctgataagg aaacatatac gtctcaggga gcaagtagcc
     7261 ttccaaattc gatgcattta cacctagact acgagtaaat tcagaactat gcacaagact
     7321 atcccagact tcgacagcta gttcgggaaa ttctttcttc aagtattgag gcatctccca
     7381 agaagctcgt ccttcaggga ttgtcacctt gcgaactgca ttttttccac tttcaaaaat
     7441 agcgaccaaa tcccttattt tttgatgtgg tggaatctcg aaccaacctg cttttagagc
     7501 acattttttg atgcgacacc atagttgaaa tgactcggga gattcccaaa tgccagaatt
     7561 ttgtaaaatt tgtgaaactt ttgccgcaga actacctttt gggatttcta aaatgaccgc
     7621 ttttgcgttg ctagaagggg tctcaatgtg cttttttacg aaaaaaacgc cagttaaaca
     7681 ggtagctaaa agaacaaaaa agataaaaat gaaaaatttc ttcataaagt caaatttaaa
     7741 gaaaaaaaac ttgtccaaag tctagaataa agcatatatt tggagaaact gatgcaaagg
     7801 gttttccctg tttagaggaa tgatatgaaa aaaattttgt tttgtgttct ggctttaatg
     7861 gttgcagttt cttttgcagc tcccaaaaaa gtgaagtcta aacgcggtga tgtggaactc
     7921 acaaaagata agagtggtgg tgctgttgtt tgtactgctg gttttaacga tgaaatgact
     7981 atcgtaaaag atggtgatac cgaagttctc gtcaaagcat cttgtggtca aggttgggtt
     8041 caaaagagtc aggtggaata tatagttgct gctgctggtg ataagtcaat gaagttagac
     8101 gacgttgacg ttattggttg gcttgataac cctagtgctg tgttcgtgtt agaacaagat
     8161 gcagcagact ttgaaggcgt aaacattgat cgtgatttta aggaatactt acaacacaca
     8221 atggaccgcg aacaagttga aatgaaaaac aacgaaaact aattttgatt ttactaagaa
     8281 acacctgcga ctattcgtgg gtgttttttt tatgaaatga atctggcttt cagacgttgt
     8341 ttaatccttt taatgcaaaa atcgatgagc aagaggcaca ggcaaatgaa acgctgaaat
     8401 tgggggtagg aacacaagca cggtattttt gggcgcaaga taaatcggca gaaaataata
     8461 aaaattggat aagctaaata ccattaggtt tattttcaat atacccaagc ccatttttca
     8521 ttgtcatgac cgcgattctc ttaacagcgt tggatttttc cttgcgaatt ttgaaatacc
     8581 attttttcat caataatttc aatggctttg tcttgaattt gtttgataaa tatacagtcg
     8641 ttttctaaag acaaatattg acgcaaaaga aagagttgtt aacgaaatag aatgttatgg
     8701 caaacatata aactaaatta ttgtagctta atttatattt aataatgctt gatcgaaata
     8761 acgcataagt atatttttaa tatagatttt tagatttctt agatgtaaaa aaaaatatta
     8821 cttctgttga gggtgatgaa aatattatat ccatcatata gagttaatta actaaaaaag
     8881 aggggaatat ggcaaaacaa aaaattgaca gcttatctta ttggagtgat ttatccgaaa
     8941 caaaaacagc tctcggaagt caggtaaatc taaaacaaga aattgttaag aatccaaaag
     9001 aagttctcaa gaatctaaat aaagacgttg ttattgcaac gaataaaaaa aatgaagatt
     9061 taacaatcgg tgatgtttta gcaaaactta atgaaaaaaa tcgaagtgct attgtatcag
     9121 tttttgttga tagcgctatt cagattgaaa aggctcagaa gaagttcatt acagaactta
     9181 cagcagttct tcctaaagat ttagcaacaa aagaggattt ctttttagtc aaaccacaga
     9241 ttccaatcaa aacgcagatt ccaatcaaaa cgcagattcc aatcaaaacg cagattccaa
     9301 tcaaaacaca gattccaatc aaaacacaga ttccaatcaa aacgcagatt ccaatcaaaa
     9361 cgcagattcc aatcaaaac
//
LOCUS       DAOOBB010000066         9196 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_9311_length_9196_cov_3.907341, whole genome shotgun sequence.
ACCESSION   DAOOBB010000066 DAOOBB010000000
VERSION     DAOOBB010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 9196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9196
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9311_length_9196_cov_3.907341"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1625)
                     /gene="mutL"
                     /locus_tag="PLT31_04450"
     CDS             complement(<1..1625)
                     /gene="mutL"
                     /locus_tag="PLT31_04450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HPW94422.1"
                     /translation="MKTAKIHILSDEIINKIAAGEVIERPASVVKELIENALDAGSTF
                     IRVQIEEGGKKKITITDNGEGMSPEDLKLCFLRHTTSKLSSADDLFHLRTNGFRGEAV
                     ASIAAISKMTIISKTKDAPCAQKVEIQSGDLKKTEEISAPQGTSFVIEDLFFNTPVRR
                     NFLSSESAESTRILDVIVRIAIAHPEIRFEYRNGSKEVFTGVEGDLRSRLAETIGTGI
                     AKKLIPLDYFENNISITGFISPSEMQNGKKNRHFLYLKNRPIWNPAVIKAVQKAYEPY
                     GKLISPTSVLFLDMPDMDFDVNVHPAKKEVRFVNDSAIYSTVHRAIRNKLQEESAACG
                     APIIHLNDCSYTSTVQEPEKKWPSFNNEISNDTLKRNFQAKPNPPKEALVQDLFSLPE
                     FGKIIPLSQKSFEIPEKAPLYSTPSFLQLAETYIVCQDMEGLLLIDQSAAHARILYEQ
                     ALKTLSSNNMINSQELLFPELIELSKVEKSLLVEILPDLKKLGFYLEPFGGDTYQIRG
                     IPSGLSFSRAEKCIHEFLDSLQEDSPSSKVPLDA"
     gene            1774..3042
                     /locus_tag="PLT31_04455"
     CDS             1774..3042
                     /locus_tag="PLT31_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94423.1"
                     /translation="MVTNLLLRIVHIELLSSYSTKDILDLIRMDSRFPSNLLDSIWFE
                     EGVFEREQHRIYLDALTEWLFSRGINPEQFIENMFSRFTSSEYMSSRIILRSYLPFVP
                     KFYETDDVRRLSLETLPDRQSLIEGAQVIFNDPVENVRNDIMIYRCEEKDKLASHYMP
                     WIEALVRYAPAFLTCPPYESIQTLCFQNTVAEVLAKHNMPILIDNKTVLLHGRVIGHV
                     VPFSEAIEKYGLSWSNTQETSIPCIRIDEDVTESGSGAVLLKRGAYYGAPANIVKISY
                     QAGVTHPDPFAKLMSSLVRQEFDVWQPVQKAHEALLNAANDSVVITYYKSDDSISVND
                     HHLMRNVPARILRNLLREYTATGREEYENREFKRDPEICMDPLRPNFESRLNRVVAHV
                     EKVSKYFEIQRHRRGGFRFKPNCRIVFKEE"
     gene            3403..4563
                     /locus_tag="PLT31_04460"
     CDS             3403..4563
                     /locus_tag="PLT31_04460"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPW94424.1"
                     /translation="MNNFQYHNPTRILFGKGEIASISSLIPPSSKVMMVYGGGSIFKN
                     GVYEQTKKALKGFDVIEFGGIEANPRYETCMRAVEQIKTQNVTFLLAVGGGSVLDGTK
                     FMALATKYTGADPWDFMLGKTATPDSALPLASIMTLPATGSEMNANFVISRDSTQEKF
                     GGYSDLIRPQFSVIDPMTTTTLPIRQTANGVVDSFVHTMEQYATFPNNGPLQDRQAEA
                     VLATLVEEGPKAMQAPEDYDVRANLCWCSTQALNGVISCGMPQDWSTHDIGHELTALY
                     GIDHARTLAIVLPGVWRFFINEKKEKLAQYGKRVLGLNGSVEECASQAIQKTEEFFES
                     LGIPTHLKDYGINASDAARMVSDRFKARNRPGFGERGLITPEVIYKIIESRA"
     gene            complement(4560..5285)
                     /gene="pflA"
                     /locus_tag="PLT31_04465"
     CDS             complement(4560..5285)
                     /gene="pflA"
                     /locus_tag="PLT31_04465"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HPW94425.1"
                     /translation="MEFGFVHSYETGAAVDGPGVRFALFIAGCPFRCTYCHNPDTWDM
                     KRGKRTSSDEVLQEIAKYASFLRIAGGLTITGGEPLTQPDFVHEIFLRTKNELKLHTA
                     LDTQGYLAAKLPDAWFDPVDLVLLDIKIMNPEKHLQITGKELQPSLDFALRLHRLQKK
                     TWLRYVLIPGITDAPEDIDALANFIASLACIERIEVLPFHNLGIHKWEELNLSYTLKN
                     QKSPSPEEVRLVQKKLDRAIQKN"
     gene            complement(5352..6854)
                     /gene="gltA"
                     /locus_tag="PLT31_04470"
     CDS             complement(5352..6854)
                     /gene="gltA"
                     /locus_tag="PLT31_04470"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HPW94426.1"
                     /translation="MSERKTIEQMDAEAKENLKKIQEIGEPLKMKDRLAIPPHAMPEL
                     EPSYRARSMEEVACGYTEAQAIAEAHRCLNCKKPFCVQDCPVHIDIPAFIAKIAEGDF
                     KAAIAKIKETSLLPAICGRVCPQERQCQLNCTVGKSNKDVLKSVGIGRLERFVADYER
                     EHGEIVVPAIKPSTGKKVAVIGSGPAGLVVAADVRREGHDVTIFEAFHKLGGVMRYGI
                     PEFRLPKSIVDREIQTLEAMGVHFETNYVVGRTRKLMDLLNKDGFDALFIGTGAGLPL
                     FMGIKGESLVGVFAANEYLTRANLMKAYDKDHADTPMWPGKNVVVLGGGNVAMDSARM
                     ALRLGAEKVRIVYRRSMSELPARGEEIHHAQEEGVEFCVLQNPSEILGDENGRVRGMI
                     VDKYELGAPDEKGRPRPVKLEGQSFEMECDTVLVAIGNGSNPLISQSTPELKVNKRGN
                     IELEDQEVNKTFVERVYAGGDIVLGAATVILAMGEGRKAAKGINEYLAKN"
     gene            complement(6857..7750)
                     /locus_tag="PLT31_04475"
     CDS             complement(6857..7750)
                     /locus_tag="PLT31_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HPW94427.1"
                     /translation="MAKIISKKQLSPAVYQIRVEAPLIAEERKAGQFIILQVDKDIGE
                     RVPLTIADADTKEGSITLIFQTVGKTTQQLATLEEGDDIPVIVGPLGSPTHVQKYGHV
                     VCVCGGVGIAPMHPIAQAMKAAGNKLTIIMGARNESLFLMKDEMGALADDLILMTDDG
                     SCGRKGLVTEPLKELCESESKPDLVIAIGPPIMMKFCSLTTKPYGVKTMVSLNSIMVD
                     GTGMCGGCRVNIGDKVKFVCVDGPEFDGHLVDWDNMMQRMSAYKNMEQEAVHRFGSHD
                     GHKCNIDKMADAKMAKDKKEV"
     gene            8275..>9196
                     /locus_tag="PLT31_04480"
     CDS             8275..>9196
                     /locus_tag="PLT31_04480"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPW94428.1"
                     /translation="MNQLIQKTELDNGVTVITDYMPEAYSATVGAWIPRGSRHEKESL
                     NGLSHFYEHLVFKGTENRSSLEIVRELEDLGGSLDAYTTRQETGFYAQVSKEDVFTAL
                     DVVGDLLMNPLFEESELEKERKVIIEEIKSYDDIAEERVGDLFNELHFEGCGLAMPIA
                     GTLKSVKSITLENLRDFRRQVIEDLPIYVCAAGNVKHQEMVDQCAIIFQKKKKGNITF
                     PLSYKANRGLKVISKQELQQSSLYLGTSFLKAELPKEFRFAFSIFNVSMGSGMSSRLF
                     QKIREENGLAYSIFSMADSYKDCYSWGVSLA"
BASE COUNT         2722 a         1712 c         1835 g         2927 t
ORIGIN      
        1 gcatctaacg gcacttttga agatggagaa tcttcttgta gcgaatctaa aaactcatga
       61 atacattttt ctgcgcgaga aaaagaaagt ccacttggaa tgcctcgaat ctgatacgta
      121 tctccaccaa aaggttcaag gtaaaagcca agctttttta aatcaggtag gatttcaact
      181 aaaagcgatt tttccacttt actcagttca attaattcag gaaacaaaag ttcttgagaa
      241 tttatcatgt tatttgagct tagtgtttta agcgcctgtt catataaaat gcgagcgtgt
      301 gcagcacttt gatcaatgag taaaagccct tccatatctt gacaaacaat gtatgtttca
      361 gcaagctgta aaaaagaggg ggtactatac aagggagctt tttctggtat ctcaaaagac
      421 ttttgagata gaggtattat ttttccaaat tcaggaagag aaaataaatc ttgaactaag
      481 gcttctttag gaggatttgg ttttgcttga aaatttcgct ttagagtatc attactgatt
      541 tcgttattaa aagaaggcca ttttttttca ggctcttgaa cggtgcttgt ataggaacag
      601 tcatttaagt gaattatggg ggctccacaa gcagcggatt cttcttgaag tttatttcga
      661 atggctctat gtactgtcga ataaatggca ctatcattta caaatcggac ttctttttta
      721 gcaggatgaa cgtttacatc aaaatccata tcgggcatat ctaagaataa gacacttgta
      781 ggagaaatta atttcccgta tggttcatat gctttttgaa cagccttaat tacagcagga
      841 ttccaaatag gacggttttt caaatataag aagtgacgat tctttttccc gttttgcatt
      901 tctgaaggag aaataaagcc cgtgatagag atgttattct caaaatagtc taagggaatt
      961 aatttttttg caattccagt gcctatggtc tctgcaagac gacttcttaa atctccttca
     1021 acaccagtaa aaacttcttt agacccgttt ctatattcaa aacgaatttc aggatgagcg
     1081 atagcaattc ttacgatcac atctaaaatt cgggttgatt ctgctgattc gctacttaga
     1141 aaattacgcc taacaggggt gttaaaaaac agatcttcga tgacaaaaga tgtgccttga
     1201 ggagcactaa tttcttcagt tttttttaaa tcgccagatt gaatctcaac tttctgagcg
     1261 catggggcat ctttcgtttt agaaataatg gtcattttag aaatagcggc aatggatgcc
     1321 actgcttccc ctctaaaacc gtttgttctt aaatggaata aatcgtcagc ggagcttaat
     1381 tttgacgttg tatgtcttaa aaaacacaat ttaagatctt ctggagacat tccttctcca
     1441 ttatcggtaa ttgtgatttt tttcttacca ccttcttcaa tttgtactct aataaaggta
     1501 cttcctgcat ctaatgcgtt ttcaatcaat tctttaacta ctgaggctgg tcgttctata
     1561 acctcaccag cagcaatctt attgatgatt tcgtcgctta aaatgtgaat tttggcggtt
     1621 ttcatgccga aaatatagac aaaactgtca gaaggctacc tttttttata gaaattgtta
     1681 aattaaacta tataaaatta cttgtttttt gtttacaatt ttaatacata caagtacgag
     1741 tgacgggaat tcctaaaaat gaggaatatg gtaatggtta caaacttatt gcttcgaatt
     1801 gtccatatag aacttctttc gtcgtattca acgaaggata tactcgattt aattcgtatg
     1861 gattccagat tcccgtcgaa tttactcgat tctatatggt ttgaagaagg cgtttttgag
     1921 cgagaacaac atcgtatcta tctagatgca ttaacagaat ggcttttttc caggggaatt
     1981 aatcctgaac agtttattga gaatatgttt agtcgtttta cctcttctga atatatgtct
     2041 tcacgaatta ttcttcgttc ttatctacca tttgttccaa aattctacga aacagatgat
     2101 gtccgtcgtt taagtttgga aactctcccc gatcgccagt cactgattga aggggctcaa
     2161 gttattttta atgatccagt ggaaaatgtt cgtaatgata tcatgatata tcgttgtgaa
     2221 gaaaaagata agcttgcttc tcattatatg ccttggatag aggctttagt tcgctacgct
     2281 ccagcttttt taacttgccc tccatatgaa tctattcaga cgctttgttt tcaaaataca
     2341 gttgcagaag tgcttgctaa gcacaatatg ccaatactca ttgataataa aacggtctta
     2401 ttacatggcc gagtgattgg gcacgtagtt cctttttctg aagccattga aaaatatggt
     2461 ctttcttgga gtaatacgca agaaacttct ataccatgca tcagaataga tgaggatgtc
     2521 actgaatctg gttcaggagc tgttttatta aaaagaggcg cctattatgg tgctccagcg
     2581 aatattgtta aaatctctta tcaagcggga gtgactcacc cagatccttt tgctaaattg
     2641 atgagttctc ttgtacgcca agaatttgat gtttggcagc ctgttcaaaa agcacatgaa
     2701 gcacttctga atgcagccaa tgattctgtg gtaattactt actataagag tgacgattcc
     2761 atttctgtaa acgatcatca tttaatgcgt aatgtaccag cacgcatttt gcgtaactta
     2821 cttcgtgaat acacagctac gggtcgtgaa gaatacgaaa atagagagtt caaaagagat
     2881 ccagaaatct gtatggatcc tttaaggcca aactttgaaa gtcgcctaaa ccgtgttgta
     2941 gctcacgtgg aaaaggtttc gaagtatttt gaaatacagc gtcatcgccg aggcggtttc
     3001 cgtttcaaac caaattgcag aatagtattt aaagaagaat agaatgtaag aaatttgaaa
     3061 cattgtttta ttattcaaat ttgttatatt tatgtgtgat gagatggtgt gataggtaaa
     3121 tcctcaaaca actctatcat tgctcccctc ctggtaagtt caaggttctg gaagccatct
     3181 cgtcattttt actcctcttt cgaggagttt ttttgtttag gggcgtaatt aaaaaaacaa
     3241 ttaattcttt acgcataaat tcatcttgat tctgtgtaat agctccaaat catttagtgg
     3301 ttacattcgt gtaagggttt aattgaaatc gttttgaaga agtaagctta ttattgataa
     3361 gttttgtaaa ttactttgca tatcacttat tctggagaac tcatgaataa ctttcaatac
     3421 cataatccca cacgtatttt atttggaaaa ggcgaaatcg cttcaatctc ttctcttatt
     3481 ccaccttctt caaaagtaat gatggtttat ggcggtggtt ctatttttaa gaatggcgtt
     3541 tatgaacaaa caaaaaaagc attaaaaggg tttgatgtta tcgagtttgg tggcattgaa
     3601 gcaaatccaa gatatgaaac ttgtatgcgt gcagtagaac agattaaaac gcaaaacgtg
     3661 acgttcttac ttgctgtggg cggaggctct gttttagatg gtacaaaatt catggctctt
     3721 gctacaaaat atactggcgc tgatccttgg gattttatgc tcggtaaaac agcgactcct
     3781 gattctgctc ttccattagc ttcgattatg acgttacctg ctactggttc agaaatgaat
     3841 gccaattttg tgattagtcg agattccact caagaaaaat ttggagggta ttccgattta
     3901 attcgtcctc aatttagtgt catcgatccg atgactacca ccacattacc tattcgtcaa
     3961 acagcaaatg gagtcgtcga ttcctttgtt cataccatgg aacaatatgc gacttttcca
     4021 aacaatggtc ctttacaaga taggcaagca gaagccgttc ttgctacgct tgttgaagaa
     4081 ggtcctaaag ctatgcaagc gccagaggat tatgatgttc gagccaattt atgttggtgt
     4141 tccacacaag ccctcaatgg ggtcatctct tgcggtatgc ctcaagactg gtctacccat
     4201 gatattggtc atgaacttac tgcattgtat gggattgatc atgctcgcac actcgcgatt
     4261 gttttacctg gtgtatggcg attctttatt aatgagaaaa aagaaaagct cgctcaatat
     4321 ggcaaaagag ttttagggtt aaacggttcg gttgaggaat gtgcttctca ggcaattcaa
     4381 aaaacagaag aattctttga atctttgggt attccaacgc atcttaaaga ttacggaatc
     4441 aatgcttctg atgctgctcg tatggtgagc gatcgtttta aggctagaaa tcgtcctggt
     4501 tttggtgaaa gagggcttat cactccagaa gtgatttaca aaatcatcga aagtcgcgct
     4561 taattttttt gtatagctcg atctaatttt ttttgaacaa gcctcacttc ttcgggagaa
     4621 gggctttttt gatttttaag cgtgtaggat aagtttaatt cttcccactt gtgaatgcct
     4681 agattgtgaa agggaagaac ttctatgcgc tctatacagg ctaatgaagc aatgaaatta
     4741 gcgagggcat caatatcttc tggagcatct gtgattcctg ggataagtac ataacgaagc
     4801 catgtttttt tctgtaggcg gtgtaatctt aaggcaaaat ctaatgatgg ttgaagctct
     4861 tttcctgtaa tctggaggtg tttctctgga ttcatgattt taatatctag taatactaaa
     4921 tccacgggat caaaccatgc atctggaagt tttgcagcta aatacccttg agtgtcgaga
     4981 gcggtgtgca gttttaattc atttttagtt cgtaagaata tttcatgaac aaaatctggt
     5041 tgtgttaagg gctctccgcc tgtaatcgtc aggccgcctg caatacgcaa aaaactcgca
     5101 tatttagcaa tctcttgaag aacttcatct gaggaggttc ttttccctcg tttcatgtcc
     5161 caagtatctg gattatggca ataggtgcaa cgaaaagggc agccagctat aaaaagagca
     5221 aaacgaacgc cagggccatc tactgcagct cctgtttcgt aagaatgaac aaagccaaat
     5281 tccataagat aagaatacaa aaaaatccct aatacacaaa tggcattagg gattaaaata
     5341 aaaatttagg tttagttttt agctaggtat tcgttaatgc ctttagcggc ttttcgacct
     5401 tcgcccatgg ctaaaataac agtagcggca ccaagaacaa tatcgccacc cgcataaacg
     5461 cgttctacga aggttttatt tacttcttgg tcttctaatt caatgttacc acgtttgttt
     5521 acttttagtt caggtgtgct ttggctaatg agaggattag atccgtttcc aatagcaact
     5581 agaacggtat cacattccat ttcaaagctt tgaccttcta acttcacagg acgtggacgc
     5641 cctttttcat caggagctcc taattcgtat ttgtcgacga tcatgccgcg gacacgaccg
     5701 ttttcatcgc cgaggatttc tgaaggattt tgaagaacgc aaaattcaac gccttcttct
     5761 tgggcgtggt gaatttcttc gccacgagca ggaagttcag acatactacg acgataaacg
     5821 atgcgcactt tttctgctcc aagacgaaga gccatacgag cggaatccat tgccacgtta
     5881 ccgccaccta agacgaccac gttcttacct ggccacatag gagtgtctgc gtgatcttta
     5941 tcgtaggctt tcattagatt ggctctggtg aggtattcgt tagcggcaaa aacaccaact
     6001 agactttcgc cttttatacc cataaataaa ggtaatccag ctccagttcc aatgaacaag
     6061 gcatcaaaac catctttatt gagaagatcc attagtttgc gagtacggcc aacgacgtaa
     6121 ttcgtttcaa agtgaacgcc catggcttct agagtttgaa tttctctatc gacaatgctt
     6181 tttggtaaac ggaattcagg aattccataa cgcatcacac cgccaagctt gtggaatgct
     6241 tcgaaaatag tcacatcatg accttcacga cgcacatctg cagcgacaac aagacctgca
     6301 ggaccagaac ctataacggc tacttttttt cctgtagaag gtttaatcgc tggaacaaca
     6361 atttcaccgt gttcacgttc atagtctgca acaaaacgtt caagacgacc gataccaacg
     6421 gattttaaaa catctttatt ggactttcca acagtacagt ttaattggca ttgacgttct
     6481 tgagggcaaa cgcgaccgca aatagcaggg agtaaactgg tttctttgat ctttgcaatg
     6541 gcagccttaa aatccccttc agcaattttt gcgataaatg caggaatgtc aatatgaaca
     6601 gggcagtctt gtacgcaaaa tggtttcttg cagtttaggc aacgatgtgc ttctgctatc
     6661 gcttgggctt cagtataacc acaggctact tcttccatag aacgagctct ataagaaggc
     6721 tctaattcag gcatggcatg aggaggaata gccaagcgat ctttcatttt taatggctca
     6781 ccgatttctt ggatcttctt caagttttct ttagcttcag cgtccatttg ttcgattgtt
     6841 ttacgttcgg acatggttat acttcctttt tatctttggc cattttggca tcagccattt
     6901 tatcaatatt gcatttatgc ccatcatgag aaccaaaacg atgtacagcc tcttgctcca
     6961 tgtttttata agcactcatt ctttgcatca tgttatccca gtccactaaa tggccatcaa
     7021 attcagggcc atcgacacag acaaatttca ctttatcgcc gatattgacg cggcaaccac
     7081 cacacatgcc tgtgccgtcc accatgatgc tatttaagct taccatggtt ttaacgccat
     7141 atggttttgt ggttagagaa cagaatttca tcatgattgg agggccaatg gctatgacta
     7201 agtctggttt agattcactt tcgcataatt cttttaaggg ttccgtgacg agaccttttc
     7261 tgccacaaga gccgtcgtcg gtcattaaaa ttaaatcgtc agccagtgca cccatttcgt
     7321 ctttcattaa gaagaggctt tcatttcttg cacccatgat aatcgttaac ttattgccag
     7381 ccgctttcat ggcttgagca ataggatgca taggggcaat acctacacca ccgcaaacgc
     7441 agactacatg accatacttt tgaacatgag taggagagcc aagagggcca acgatgacag
     7501 gaatgtcatc gccttcttct agagtggcta gttgttgagt cgttttacca acggtttgaa
     7561 aaatcaatgt gatagagcct tcttttgtat cggcatcagc aatcgttaac gggacgcgtt
     7621 ccccaatgtc tttatcgact tgaagaatga taaattgccc agctttacgt tcttcagcaa
     7681 tcaaaggagc ttcgactcga atttggtaaa cggcgggaga aagttgtttt ttagaaataa
     7741 tttttgccat agtacatcaa agttacattt ttgacgaaaa aaaataaagt gaaaactctg
     7801 tacttgggga taaaaaacgt tatttgaatt agagttcatt tagtatgctt tttttgctcc
     7861 acagggcgtt attttaagca tttgtattga aaaaaagcta ttgtctcaaa tgcaattttt
     7921 tgtataatag gccgttaatt ataacggcga gtccatttgc catagaaaac tttcgaagag
     7981 caatcgtcac agggagagac gttgtatttc aagatattct taaaataggg gcctcttttg
     8041 aaaggatcat atcgtaagta tccataaaaa atatcaaatt gatattggtc tgtatttata
     8101 caacattgct tttctgagag aactctataa ataatggagt cttgagtcac tgtttctaaa
     8161 ataagaagtg taggttaagg cttttccaca tcaaaaagaa tgaagaaata aaagaagagt
     8221 tacaaaatta ttgtaaattg tactgcgttt atatccgaaa caaggtttgt tgtgttgaat
     8281 caattaattc agaagaccga attagataat ggtgttaccg ttattacaga ttacatgccc
     8341 gaagcatatt ctgcaactgt tggcgcttgg attccaagag gctctcgtca tgaaaaagaa
     8401 tctctcaatg gattaagtca tttttatgaa catttggtgt ttaaagggac tgaaaataga
     8461 tcttcattag aaattgtgcg agaattggaa gatttaggtg gttcattgga tgcttatacg
     8521 actcgtcaag aaacgggctt ttacgctcag gtgagtaaag aagatgtgtt tacggcgtta
     8581 gatgtggtgg gcgatttgtt gatgaatcct ctttttgaag agtcagagct cgaaaaagag
     8641 cgaaaagtca ttatagaaga aatcaaaagc tatgatgata tcgctgaaga acgagtaggt
     8701 gacttattta acgaattgca ttttgaaggg tgtggtttag cgatgccaat tgcgggtaca
     8761 ctgaaatctg ttaaaagtat tactctagaa aatttgcggg attttcgtcg acaagtgata
     8821 gaagatttac caatttatgt gtgtgccgca gggaatgtga aacatcaaga aatggtggat
     8881 caatgtgcaa tcatttttca aaaaaagaaa aaaggaaaca tcactttccc tttaagttat
     8941 aaggccaatc gaggccttaa agtcattagc aaacaggagc ttcaacaatc tagcctgtat
     9001 ttaggaacaa gtttcttgaa agcagagctt cccaaagaat ttagatttgc cttttctatt
     9061 ttcaatgttt ctatgggatc gggcatgagt tctaggttgt tccaaaaaat tcgagaagaa
     9121 aacggactag cgtattccat attttcgatg gcggattctt ataaagattg ttatagctgg
     9181 ggcgtttctt tagcca
//
LOCUS       DAOOBB010000067         9058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_9472_length_9058_cov_4.192602, whole genome shotgun sequence.
ACCESSION   DAOOBB010000067 DAOOBB010000000
VERSION     DAOOBB010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 9058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9058
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9472_length_9058_cov_4.192602"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PLT31_04485"
     CDS             complement(<1..440)
                     /locus_tag="PLT31_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94429.1"
                     /translation="MTHFNDYNESSTTAKSISIILIVLSVVYSLFPNIPFPLDDITLF
                     IAAVSNAGYHLLNPQQIILKKVMDVLRKILFVIYIAIIVLGGLILLITLHFAGVDFKN
                     ALWIYVFILAALLFIGRLFQNKLVGWIGEVYSASCIRMITGGVVL"
     gene            481..1416
                     /locus_tag="PLT31_04490"
     CDS             481..1416
                     /locus_tag="PLT31_04490"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HPW94430.1"
                     /translation="MSLLDSLFGKRLGPVFVKDASDASDYIEKMKELSSKASGSLKDK
                     MEEQIRNAEAGLYGEKQIIYELKNSGLDMMIAHDLYLKKGELSAQIDFLVMTRKHLFV
                     IECKNLYGDIEIDDKGNFIRHIRYGKTYKKEGLYSPITQNERHLNVLKEVRRLVKNNL
                     LGKLFYDTAFPKCYRSIVVLANEKTVLNDKNAPKQIRDQIIRADQLISYIKKVDSSDD
                     NYESSDKEMEEVMFFYVNCSIPNQSDYAKKYEAMLEEFSLSNPTSKIDEPMSDEKICS
                     RCGSPMVFRTAKKGAHSGNQFWGCSNFPKCRFMIK"
     gene            1876..4896
                     /locus_tag="PLT31_04495"
     CDS             1876..4896
                     /locus_tag="PLT31_04495"
                     /inference="COORDINATES: protein motif:HMM:NF024486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HPW94431.1"
                     /translation="MSISRFIFRAIHEGRWLSIEYVNAENKTTYYWISISDIKIVNRD
                     GVDRAKLVVMGLHLKTHVCTRLEIFFDGIKQAQIIEGSYAARNNSLLHELNENPTKYS
                     CLFSNIHNIKILTYLQECQKLSSDNYEKSFGLLEHLDESKFLNGCYQLTQEECDFLID
                     YFKKKASKKSNSDFTPIELFGLNTLSIKTLKGLYVLAFKELLFDIQNKQLILSKNSRV
                     LTRFILKNQTLDNVVKVSNYLYEEDLYLLKDFDENEKTIKELILKHGNLKSHELLDDM
                     PHIIALESKSPDLTIEYDAITKSVSDGSVSTPLRAFFGDLLRPSRNTKNYPIVLMDDQ
                     VNLDQLLAIHKSLKYPITYIQGPPGTGKTKTIANTMISALFNKKTVLLSSQNNHPING
                     AFKILSSITIDDKIIPFPILRLGNNDEVKKSIQYIKNIFEYTKSWTIKNKALDDFTEI
                     KIENTAKLAEYLENYEDKLDLLERKETIEDFLNQNQNFNVNMNVQSKQLREIEKQISA
                     FEEISDEKVKFLLPNDFQKFLFYLRYLSAQYIQRLKEPKYKELLKILELEDQDDAVKK
                     LNHYLREDGNLHHLLRVFPIIATTNQSAKKLASPSIHFDLTIVDEASQCDVAMALLPI
                     LRGQNLMLVGDPQQLNPVITLDDLDNNVLIRKYNIAPEYDYKNNSIYKTFLAADSISD
                     EVLLSHHYRSAKEIISFNNKKYYNNKLLVKTEKRDLPPLIFQETPNENPVDRNTSYNE
                     ALSIVDYVKKYPDKSIGVITPFVAQKARINMALIERGLEPLCGTVHEFQGDEKDIILF
                     SFAITQKTSSRTYEWFSKNKELINVAVSRAKEELVLFSNTETLNRLHTQADDLYELVQ
                     YVKTNGDYKVTPKHSHSRALGLKPYSTKTESDFFININHVLSVVDEQKKYTIKTEVPI
                     AQVFENNTSGNTLFYSGRFDFVLYEKDGRTEIPILAIELDGEEHRTNENRIKCDQAKE
                     LICDTHDFTLIRIPNSYARRYSHIKEILVSYFKK"
     gene            4934..5371
                     /locus_tag="PLT31_04500"
     CDS             4934..5371
                     /locus_tag="PLT31_04500"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HPW94432.1"
                     /translation="MKKILDLCFNPGEYSNNISLSLLFLRLIVGGFMLTHGIGKWMML
                     IGDGPIRFADPIGIGVTASLALTVFAEVFCSLFLIFGFATRLAVIPLVITMLVATIVV
                     HGADGFGKQELPLLYASIYVFLAVMGSGKFSVDQYLYKKIPTD"
     gene            complement(5511..7208)
                     /locus_tag="PLT31_04505"
     CDS             complement(5511..7208)
                     /locus_tag="PLT31_04505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HPW94433.1"
                     /translation="MSQIMNVVVFLAGGLGLFLMGMKFMSDGLQTVAGPSLKTLIGAV
                     TNNRILGVLVGVLVTCLVQSSSITSVMVIGFVNAELMMLKQAIGVIMGANVGTTVTGW
                     VLVLDIGKYGLPLAGVFGLIYCFVSKERIKYSALAILGVGLIFMGLEAMKAALAPLAN
                     SPEFCDFLKLFEASTFSGLIKCVLVGVILTAIVQSSSAMLGVTIAMAHQGLIGFETAA
                     ALVLGQNIGTTITAFLASIGASRAARRAALFHILFNVFGVLWVIAIFRSYLGFVEYVL
                     EAVFNITDPNMIKTENGVSVAPYMTASIATFHTIFNVVNLLVLLPFTGFIATALEKLV
                     KKKVVKKKIVATKLDFPLLASPFAAITQSNREILLMNEKLKTMMGDLRVCLTNGPDVR
                     KKQDAIFGAESHLDIAQEEITNFLTNLLSNRASLAVANDAERQLRMSDDLETASDYIT
                     QILKLHLRMSEKNVAFDEEHSSGILKLHDETTLLVDRVQELLEQANKDERNLSVIRKQ
                     GFDITQLVRDLRSMHWSHLAECQYDPLVTTTYTDILNDYRKIKEHLIVAAETGLGVK"
     gene            complement(7356..7937)
                     /gene="coaE"
                     /locus_tag="PLT31_04510"
     CDS             complement(7356..7937)
                     /gene="coaE"
                     /locus_tag="PLT31_04510"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HPW94434.1"
                     /translation="MLRVGITGSIGSGKSAVGRILSEKGYPVLDADLLVHQLYASNSL
                     LRESLSARFGKEILTPDGVDRSKLKTLVFQDDQARLDLEEIVYPVLEQEQNRILSEWE
                     GSFSVAFVEAALLYRLPHFVDSLFAVWVVSASEEVRLERLIQRGLSLMEAKSRIELQK
                     TFPLPNHKHIFYIQNDSSLVELADEINKRLVFL"
     gene            8049..8933
                     /locus_tag="PLT31_04515"
     CDS             8049..8933
                     /locus_tag="PLT31_04515"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 family protein"
                     /protein_id="HPW94435.1"
                     /translation="MNQKTISPKTLAIILKMQQTEENASAIYFKIASQVKDQNKAIIE
                     QIAKDEAQHALVWSKYTKKKLKPNRLYVFWVSLLAKLLGFTFIVKFLEKGEASGILAY
                     QSIMDEIPEVSRIVSEEADHENKLMGMLNEERLKYVGSMVLGMNDALIELTGALAGFT
                     FAMQNTRIVALAGLITGVSATLSMTSSSYLSSKADGDPDALKSSLYTGAMYLATVALM
                     VFPYLIVPNSMPLVALSIMLAIVVFIIFAFNFYIAIAKDLHFKSRFLEMLILAILVTG
                     VSFAIGTVFKSILNIDIL"
BASE COUNT         3045 a         1657 c         1530 g         2826 t
ORIGIN      
        1 aggacaacac ctccagtaat cattcgaata caacttgctg aatacacctc tccaatccag
       61 ccgactaact tattttgaaa aaggcggcca ataaataata gagctgctaa aataaaaaca
      121 taaatccaca aagcattttt aaaatcaact ccagcaaaat ggagcgtaat aagaagaatt
      181 aacccaccta agacaataat cgctatataa ataacgaaca aaattttccg taaaacatcc
      241 atcacctttt tgagaattat ttgttgtgga tttaacaaat gataccctgc attagaaaca
      301 gcagcaataa acaaggtaat gtcatctaga ggaaaaggaa tgtttgggaa aagagaataa
      361 actacactta gcactataag aataattgaa atacttttcg ctgttgtaga agattcatta
      421 taatcattaa aatgggtcat tattttttta atataattta tattttcgga gaggtatgca
      481 atgtcattat tagattcatt atttggaaag cgtttagggc ctgtttttgt gaaggacgca
      541 agtgatgcaa gtgattatat agaaaaaatg aaagagctct cttcaaaagc ttctggatct
      601 cttaaggata aaatggagga gcaaattcgt aacgcagaag ctggtctcta tggcgaaaag
      661 cagattatct atgagttaaa aaatagtggc ctagatatga tgattgcaca tgatttatat
      721 ttgaaaaagg gagaattatc tgcgcaaatt gattttttag tgatgactcg aaagcatctt
      781 tttgtaattg aatgcaagaa tctttatgga gatatagaaa ttgacgacaa aggtaatttt
      841 attcgtcata ttcggtatgg taaaacttat aaaaaagaag gtctttattc ccccatcact
      901 caaaatgagc gtcatcttaa tgttttaaaa gaagtgcgtc gactggtgaa aaacaattta
      961 ttaggaaaac ttttttatga caccgctttt ccaaaatgtt accgttcgat agttgttctt
     1021 gccaacgaaa aaactgtttt gaacgataaa aatgcaccta aacaaattcg tgatcagatt
     1081 attcgtgcag atcaacttat ctcttacata aaaaaagttg actcttctga tgataattat
     1141 gagtcttccg ataaagaaat ggaagaagta atgtttttct atgtgaattg cagtattcca
     1201 aatcaatctg attatgccaa aaaatatgaa gcgatgttag aagaatttag cctctcgaat
     1261 cctacatcca aaatagatga gcctatgagt gatgaaaaga tatgctcgcg ctgtggaagc
     1321 cccatggttt ttagaacagc aaaaaaaggc gctcattcag ggaatcaatt ctggggatgt
     1381 tccaatttcc ccaaatgccg ttttatgatt aaatagtacc tacaaacatg cagtcatata
     1441 gactggaata taggtaaacc cttctttttc cataaagtaa tccatagaat aatgccttct
     1501 gtaattagaa gacttgactt caatgggaca aatttttccg ttacgaatca caagaaaatc
     1561 tatttcgtat ttcttttctt gtttttttga tcttggtgcg tgtatttaaa attctgaaaa
     1621 tataaatcgt gtccttgact tttaagcatc tgagccacaa tgttttccat caacattcca
     1681 aaatttgttc ctcctttgtc aaaaaagaat tctttataca ggtttcttcc gtttctgtta
     1741 tagttcatca tttgtgttac gaaaagtcct gaatcaccca aaaaatgttt atttttaaaa
     1801 atagattaac ccaatctatt cgtttaatat tgcttgatgt tttctttatc tctattttga
     1861 ggatatattt gatttatgag tatttctaga ttcattttca gagcaattca tgagggccgt
     1921 tggctttcta ttgaatatgt gaatgctgaa aataagacga cttactattg gattagtatt
     1981 tctgacatta aaattgtgaa tagagatggg gtggatcgcg cgaagctcgt ggtgatgggt
     2041 cttcatttga aaactcatgt gtgcactcgg ctagagatat ttttcgatgg gattaagcag
     2101 gctcaaatta ttgagggttc ttatgcggca agaaataatt ccttgcttca tgaacttaat
     2161 gagaatccaa caaagtattc ttgtcttttt tccaacattc acaatatcaa gattttaacg
     2221 tatttgcaag aatgtcaaaa attgtctagt gataattatg agaagagttt tggcctttta
     2281 gagcatctag atgaatcgaa gtttttgaat ggttgttatc agctaacaca agaagaatgt
     2341 gattttttaa ttgattattt caaaaagaag gcttcaaaaa aatcaaatag cgactttacc
     2401 cctatagaac tttttgggct gaatacgtta agcatcaaaa ccctcaaagg gctttatgtt
     2461 ttagcattca aagaactgct ttttgatatt caaaataagc aattaatttt atcgaaaaac
     2521 tcgcgagttt tgactcgttt tattcttaag aatcaaacgc tagataatgt tgtcaaagtt
     2581 tcaaattact tgtacgaaga agacctatat ttattaaaag attttgatga gaatgaaaag
     2641 accatcaaag agcttatttt aaaacatggg aatttaaaaa gccatgaact ccttgatgac
     2701 atgccgcata ttattgcgct tgagtccaag tctcctgatt tgaccattga atatgatgct
     2761 attacgaaat ctgtctctga tggttctgtg agtactcctt tacgagcctt ttttggcgat
     2821 ttactacgcc cttctcgaaa cacaaaaaac taccccatcg tcttgatgga tgatcaagtt
     2881 aatctagatc aactacttgc cattcataaa tcactgaaat atcctattac ttatattcaa
     2941 gggcctccag gaactggaaa aacaaagact attgccaata cgatgattag cgctcttttt
     3001 aataaaaaga ctgtcttgct ttcctctcaa aataatcatc cgattaatgg ggcttttaag
     3061 attttatcaa gtatcaccat tgatgataag ataattcctt ttcccatttt acggcttgga
     3121 aataatgatg aagtgaaaaa gtctattcaa tatattaaga atatttttga atacacaaaa
     3181 agctggacaa tcaaaaataa ggcattagac gatttcaccg aaattaaaat tgaaaataca
     3241 gctaagctcg cggagtacct tgagaattac gaagacaagc ttgatttact agaacggaaa
     3301 gaaactattg aagatttttt aaatcagaat caaaatttca atgtcaatat gaatgtgcag
     3361 agtaaacaat tacgggaaat agaaaagcaa atttctgcat ttgaagaaat ctctgatgaa
     3421 aaggtcaaat ttttattgcc taatgatttc caaaaatttc tattttattt acgttatttg
     3481 tcagcacaat atattcagcg cttaaaagag cccaaataca aagagctttt gaaaattcta
     3541 gaattagaag accaagacga tgccgttaaa aaactgaatc attatttaag agaggatggc
     3601 aatttacatc atttattgag agtattcccc atcatcgcca ccaccaatca gtccgctaaa
     3661 aagctagcct ctccaagcat tcattttgat ttgactattg ttgatgaagc aagccaatgt
     3721 gatgtagcca tggcactatt acccattctg cgtggacaaa atctgatgct cgtaggcgat
     3781 cctcaacaat taaatccggt aatcacttta gatgatctcg acaataatgt gttaattcgg
     3841 aaatataata tcgctccaga atatgattat aaaaacaact ctatttataa aacattccta
     3901 gctgctgatt ctattagtga tgaagtctta ctaagccatc attatcgttc cgccaaagag
     3961 attatcagtt ttaataacaa aaaatactac aacaataaat tgctcgtcaa aacagaaaaa
     4021 agagacttac ctcccttaat ttttcaagaa acacccaatg aaaatcctgt ggatagaaac
     4081 acttcttata atgaagcatt gtcaattgta gattatgtaa aaaaatatcc agataaatcg
     4141 attggtgtaa ttaccccttt tgtagcacaa aaagcaagaa ttaatatggc attgattgag
     4201 agaggattag agccactctg tggaaccgta cacgagtttc aaggagatga aaaggatatc
     4261 atcttgttct cttttgccat cacacaaaaa actagttctc gtacttatga atggttttct
     4321 aaaaataaag aattgatcaa tgtggctgtt tcgagagcga aagaggaact agttctcttt
     4381 tctaatacag aaactctcaa taggctccat actcaagcag atgatcttta tgaattagta
     4441 caatacgtca aaaccaatgg cgattataag gtaacgccca agcattctca ttcaagagct
     4501 ttagggctta aaccttatag cacaaaaaca gaatccgatt tttttataaa tatcaatcat
     4561 gtgctttccg tggtggatga acaaaagaag tatacaatca aaactgaagt tcctattgct
     4621 caggtttttg aaaacaatac tagtggaaat actctttttt attctggtcg atttgatttt
     4681 gttttatatg agaaagatgg tcgcacagaa atacccattc ttgctattga attggatggt
     4741 gaagaacata gaactaatga aaacagaatt aaatgcgatc aagccaaaga gctcatttgt
     4801 gatacccacg atttcactct gatacgcatt ccaaattctt atgcaagacg ttatagccat
     4861 ataaaagaaa tactagtgag ttatttcaaa aaataaatta cttttatttt actaaaccaa
     4921 aaaagggagt cttatgaaaa aaatcctaga cctatgtttt aatcctggtg aatactccaa
     4981 caatatttcg ctttcattac tttttcttcg cttgattgtt gggggtttca tgctcacgca
     5041 tggcatcgga aagtggatga tgctaattgg cgatggtccc attcgatttg cggatcctat
     5101 cggcattgga gttactgctt cgctcgcact aaccgtcttt gcagaagttt tttgctcact
     5161 atttttaatc ttcggctttg ccactcgctt agcagtgatc cctctagtca tcacgatgtt
     5221 agttgccact attgtggtac acggtgccga tggttttggt aagcaagaat tgccattgct
     5281 ttatgcatca atttatgtat ttcttgccgt gatgggttct ggaaagttct ccgtcgatca
     5341 atatctttat aaaaagatac ccactgacta agcgggttta taaagttcac ataatcatcc
     5401 agtttatgca aaacgatatt gcgtggacgc tccctgcgac caatatgtgg acgtgatcaa
     5461 ggacattttt aagccaaact ctcggttttg gagaaataca aaaaagtaag ttatttaact
     5521 ccaagaccag tttctgcagc aacaatcaaa tgttctttaa tttttcgata atcatttaat
     5581 atatctgtat atgttgttgt cactagagga tcgtattggc attcagccaa atgactccaa
     5641 tgcatacttc gtaaatctcg gactagctgt gtaatatcaa aaccctgctt gcgtattaca
     5701 gatagatttc gttcatcttt atttgcttgt tcaagcaact cttgcacacg atccacaagg
     5761 agagtcgttt cgtcatgtag ctttaatatt cctgacgaat gttcttcatc aaaagccaca
     5821 tttttttctg acattcttaa gtgcaatttc aatatttgcg taatataatc agacgctgtt
     5881 tcaagatcat cactcatgcg taactgacgc tctgcatcat tagcaaccgc cagagaagct
     5941 cgattagaaa gcaagtttgt taagaaattt gtaatttctt cttgtgctat atctaaatga
     6001 gactctgcac caaaaatagc atcctgtttt ttgcggacat caggaccatt cgttaagcat
     6061 acacgaagat cacccatcat cgttttaagc ttctcattca ttaacaagat ttcacgattc
     6121 gattgagtaa ttgcagcaaa aggagatgca agtaaaggaa agtccaattt tgtggcaaca
     6181 attttctttt taaccacttt tttcttaacc agtttttcca aagcggtagc aataaagcct
     6241 gtaaatggca ataaaactaa aagattcact acattaaaaa tcgtatggaa tgtggcaatc
     6301 gaagccgtca tataaggcgc cacagaaaca ccattttccg ttttaatcat attaggatca
     6361 gtaatattaa ataccgcttc aagaacatac tccacaaatc ctaagtacga cctaaatatg
     6421 gcaatcaccc ataatacacc aaatacgttg aataaaatgt gaaataaagc cgctctacga
     6481 gctgcacgag atgctcctat ggaagcaagg aaagcagtaa tcgttgttcc aatattttgt
     6541 cccaaaacta gagccgccgc cgtttcaaaa ccaatcaacc cttgatgagc cattgcaata
     6601 gttacaccaa gcattgcaga agaagactgt actatggctg ttaaaataac tccaacaagt
     6661 acacatttaa tcaaaccaga aaaagtactc gcttcaaaga gttttaaaaa atcacaaaat
     6721 tcaggggaat tcgccagagg agcgagagcg gctttcatag cctctaagcc cataaaaata
     6781 agacccacac ctaagatagc aagggcagaa tacttgatac gctctttact aacaaaacaa
     6841 taaattagcc caaatacacc tgcgagaggt aaaccatact tacctatatc taaaactaga
     6901 acccagccag tgactgttgt tccaacattg gcccccataa taacaccaat cgcttgcttg
     6961 agcatcatta gttctgcatt cacaaaacca ataaccataa cagaagtaat agaactcgat
     7021 tgaacaagac aggtgactaa aacaccaacc aaaacgccta aaattcgatt attagtgact
     7081 gctccaatca aggtttttaa ggaaggacca gcaaccgttt gcaagccatc ggacatgaat
     7141 ttcatgccca ttaaaaaaag tcctaaacca ccggctaaaa aaacaacaac attcattatt
     7201 tgggacactt gtgcctcttt ttttcttttt ccatctattt agttttaaat ttagaatttg
     7261 gtttggacat ttcaataaaa aatagatgca ttatgttgta ttttttttgc ataaaaaatc
     7321 accttttttg aaaagaaatt aaaacaggcg atttttcaca aaaaaactaa ccttttattt
     7381 atttcatcag caagctctac taaagagcta tcgttctgga tataaaaaat atgtttgtga
     7441 ttaggtaaag gaaacgtttt ttgcaattca attcggcttt tcgcttccat taaagaaagc
     7501 cctctttgaa tcaatcgttc taaacggact tcttcagaag cagaaacaac ccaaacagca
     7561 aaaagactat ctacaaaatg agggagtcta tataataagg ctgcttctac aaaagctacc
     7621 gaaaaagatc cttcccattc agagagaatt cgattttgtt cttgctctaa aacgggatat
     7681 acgatttctt ctaaatcaag cctcgcttga tcatcttgaa aaacaagcgt tttcaattta
     7741 gaacgatcaa cgccatctgg agtaagtatt tcctttccaa aacgagcaga aagagactct
     7801 cgcaaaaggg aattggaggc gtaaagctga tgaactaata aatcagcatc taaaacgggg
     7861 tacccctttt cagaaaggat acgccctacc gcacttttcc ctgaacctat cgaacctgtg
     7921 attcctacac gaagcatgtt tctaaattta acttatgtat atggttcaga gggtattatt
     7981 ttagtccact agcctctttt ataggctaag tcttttttat tcacttcctt ttattctatt
     8041 ttaaaaatat gaatcaaaaa acaattagcc ccaaaactct cgctattatt cttaaaatgc
     8101 agcaaactga agagaatgct tctgccatct attttaagat agcaagtcaa gttaaagatc
     8161 aaaacaaagc gattatagag caaattgcaa aagatgaagc tcaacacgcc cttgtttggt
     8221 caaaatacac gaaaaagaag cttaaaccca accgtcttta tgttttttgg gtgagccttt
     8281 tagcaaaact ccttggtttc acttttatag tgaaattttt agaaaaaggg gaagcctcag
     8341 gaatattagc ctatcaatct attatggacg aaatccctga agtttctcga attgtgagtg
     8401 aagaagcaga tcacgaaaac aagcttatgg ggatgcttaa tgaggaacga ttgaaatacg
     8461 ttggctcgat ggttctaggc atgaatgacg ccttaataga actaacagga gctcttgctg
     8521 gctttacatt cgccatgcaa aacactcgaa ttgtggctct agcaggttta atcactggtg
     8581 tttcggcaac actttctatg acatcttctt cgtacctttc atccaaagcc gatggagacc
     8641 cagatgcact aaaatcttct ttatatactg gggccatgta tttagccact gtagctttaa
     8701 tggtattccc ctatttaata gttcctaatt caatgccttt agttgcgtta agtataatgc
     8761 ttgccattgt cgttttcatt attttcgctt ttaactttta tattgcaatt gcaaaagatc
     8821 tccatttcaa atccagattt ctggaaatgc tcattttagc gattcttgta acgggagttt
     8881 cttttgctat cggcactgtg tttaagtcga ttcttaatat agacattctc taatttggga
     8941 ggccttcttg gattcaaatc gctatgtacg ccaaatcgct cttcctaaca tccaagaaga
     9001 agggcaaaaa ataattagtt ctttacggat cttagtcttt ggctgtggtg cactcggc
//
LOCUS       DAOOBB010000068         8962 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_9609_length_8962_cov_3.963624, whole genome shotgun sequence.
ACCESSION   DAOOBB010000068 DAOOBB010000000
VERSION     DAOOBB010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 8962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8962
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9609_length_8962_cov_3.963624"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(401..3973)
                     /locus_tag="PLT31_04520"
     CDS             complement(401..3973)
                     /locus_tag="PLT31_04520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibro-slime domain-containing protein"
                     /protein_id="HPW94436.1"
                     /translation="MIFGNDTIPLQPAVEGSDYCGWFSYTFQSPSVFIPVYFQRPHSS
                     FTFPASQSTIDIGPLISEGKTVFIDGTASEVRLDTVRTSKGECFNGDYSFHLYWTGNS
                     EIQINVGTTISNTTIHKQSNMDNWYLHDAENVGSGSDFVVFNMRDYADNTTSTFSTTQ
                     TISEIFPKGVYDAWFRIESDDRIVITNTPIYQKRIRILNPWSNTSPQMIVNGDTLGMK
                     TVKNYCGWFEATYQGSIDDFHLLFKQSIGTEIYSDAGRTDGFPMTLDSIFSIADTIWI
                     RPTPYPSGRPTFHSSFPNILGDCPIKTLAVMMFDWYDGSMEEGPYGHVAQGVPQYGIG
                     TDADFGANGCEEGTPGGNGATQGMVELFLGPNEVPVRSASFPETKCTNANNLNKWFLP
                     ETLSVVNGTVYTNATCRDLTLELDEEGLWLGQKDDSSPEHGFFLLDDFKFLDPEGLIP
                     NPKYDSASGEGGDHNFGMTMKAVAEFEYVPGQYFEFNGDDDVWVFINNRLVVDIGGQH
                     NKVFGSVNLDTLGLTPGETYPFHIFYAERKRYKSNFMMRTSMDLRTERSYFPTDVSPS
                     PEIIKYEIWQIVREQSLACDFSGMKDKEKTYAPSDFNLIGGNLPLEGVWLQEGAHYGG
                     IVIDSGMAGFTIDTSLIRINRSLAPGTYCLRFNLQADQSHSGEVWFTVAEYPRPSIAF
                     ANDDWEIISADTSVIGEWAHTLYPVRVRIFEENCAGCDGNISLFSSDPNLKFMDSLGN
                     PLTESSIVNGQVTFWILGTQEINGAYIQVLGDYYANILLWTDINLKVPPVPQLEFAEM
                     HDRNGDGIPDSLHLKYTEPLIGSKFPDSLSWRYGDSLMRFVNKTELQAAFEETSSIVL
                     LSDGFTNKVFTGLLDEPYFGFSLTSFTYNPEGNEDKIIFDMAGQIQDKVGPIILSATV
                     KEKSGNVTVLSLTFSEALQKDDYLLLGDLFKFRYWRDGHLNELPMIPQSSTASDLHRY
                     DLYFVESDGLDIPAVGDSIRFNPGIATDLSGAHPHDLNPWVRIVGEQKTKISSTTLIS
                     VNPLDDRLINSPTVTTHLIEKNVTKDELMRTYGKHGHIIDVDLEEYLRNLIAEGDSTL
                     TMDSLSVIFETFYFSTIGQYVNSSSGQINCNDSIYGGNCQVNKGNIFVAWNMKSHINR
                     WVGSGAYIGRITVKIRARKKIVSEETEDFIWGVQRIRGKGVLIE"
     gene            complement(4127..4384)
                     /locus_tag="PLT31_04525"
     CDS             complement(4127..4384)
                     /locus_tag="PLT31_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94437.1"
                     /translation="MNKNIISCVYLLVLFFANALMAQPYILHIQSPWASDDPHSFKAG
                     RFTENTTYAMTANGNYWHTVEITVAEVTEYSTTTIELSSGE"
     gene            4654..6237
                     /gene="pta"
                     /locus_tag="PLT31_04530"
     CDS             4654..6237
                     /gene="pta"
                     /locus_tag="PLT31_04530"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF004167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HPW94438.1"
                     /translation="MRKVYVIAPTGINAETAKTAHSFYDYIVKQEPKTVYFKPIGESH
                     GSISQERVRVLLSSGNSSVLMEQLLALFMEVASDQEVIVVEGVSANEFPFRSKKLNEI
                     IAQALDASVILVSSATGKTPLAVCSEVEIEAQDYFEIKVRVACSVITGVEESAKESLS
                     QAFKNSFVNVLAYEINNEMKIDESLVKKIIELKTEHHITQPEFRANLIRQAGLNLKRI
                     VLPEGTEPRTVKAAILAYERKIALPVLLGNRTEIEYIAEKHGLDVPAGLEIIEVNETT
                     GERFVNTLVELRKEKGMTEELARDLLKDSVWIGTMMLKMGEVDGLVSGAVHSTADTVR
                     PALQVIKTAPGVKSISSVFFICLPSQILVYGDCAINQNPDSSELASIAIQCHDTAKAF
                     HLPAKVAMLSYSTGTSGKGDDVDLVVEATRLVKSVRPDIVIDGPLQYDSATAESVAKI
                     KAPHSPVAGKANVLVFPNLNAGNITYKAVQRSAANVICIGPMLQGLAKPVNDLSRGAF
                     VDDIIYTIALTAIQAQSID"
     gene            complement(6234..6995)
                     /locus_tag="PLT31_04535"
     CDS             complement(6234..6995)
                     /locus_tag="PLT31_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94439.1"
                     /translation="MVFRALTILLLWVWGIGVANEDLRIGDSCYSLRAEKANGDKADT
                     NNVKKMIQAYERAFAAADTLIQEKAAEGLLKSYYFMLRFASPEKDKRKDVLNEVTLLS
                     EKIHSLYPNNRNITKIYIVILSMWGAETNPLIAVKQGVAKKVRDLADSIQDYQILGRS
                     HQILPYIPIILSWPDKKLAEKYLKMSLEQDSKDPYNYFFLSELRFDQGLYDEAEKIIQ
                     AGLDLGVRTDFFLEDKRGRWHLKELQKKVRNKKRR"
     gene            complement(7005..7148)
                     /locus_tag="PLT31_04540"
     CDS             complement(7005..7148)
                     /locus_tag="PLT31_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94440.1"
                     /translation="MLKKEEKTLIRTALMEYRNLLFKAFHGTAEEKARIAQVNKLLQT
                     WKV"
     gene            complement(7199..8548)
                     /locus_tag="PLT31_04545"
     CDS             complement(7199..8548)
                     /locus_tag="PLT31_04545"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HPW94441.1"
                     /translation="MTLYDIISDPDQYDLSIQTVGTPTLHSPMMGMRFISNTERVSLC
                     TDVQKIESLLATGSHLPSLEAAGPREMIYHDPAWTRAAIVTCGGLCPGLNNVIKGLVQ
                     VLWFDYGVQNIFGIPYGYRGLNPSYGLSPVVLTPEVVDAIQDDGGTILGSSRGEQDVS
                     VMVDTLMRLNINMLFCIGGDGTLRGAREIAAEIKRRKQPISVIGIPKTIDNDLNLVDR
                     TFGFETAVLSATDVITSAHNEANGVFNGLGLVKLMGRDSGFIAAYASLATTVVNFCLI
                     PEVPFVLEGENGLLHALENRYSIGKTHAVMVVAEGAGQHLFKNIPERRDASGNILKND
                     IGDFLTEKIEAHFKSIGKEINIKYFDPSYTVRSIPAKGTDAIFCFQLAENAVHAAMAG
                     KTNMVVGSMNNQFTHVPIDYAVSERRKIEPNNALWHAVLGATRQQDYFSGKRHNRKK"
     gene            complement(8689..>8962)
                     /locus_tag="PLT31_04550"
     CDS             complement(8689..>8962)
                     /locus_tag="PLT31_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW94442.1"
                     /translation="YKMIVLELRVDGNDIFFVVRDYGPGIPGSELTKVFEEFYRVENE
                     MTRKTNGTGIGLSMVKKLCSLTNMRIEIENASPGLRTKIHFPPISI"
BASE COUNT         2867 a         1714 c         1670 g         2711 t
ORIGIN      
        1 gtttctgtgc ccatcacaat attgggtata gtcttatttc cccaaggact tttaaaccaa
       61 atggtggttg ctgtttgtga ccaagcaagt cctgttagca ttaaaatctc taaaaaaatc
      121 agagaaaaaa tttgtttttt catcacacac caactccttt aaacaggata aagaccttcc
      181 caaaatgtcc ttataagtta cttaagtaat atatatttaa tacttaccat atccaaaaac
      241 caaaatgctt aagaaattag tttacgtgta aaaatagttt aacatatcga ataaattaaa
      301 aaacgataca aattaccccg aaacataatt ggaatgcatc actgcataaa aattggtgaa
      361 aaaatgaaaa aagaataaaa acggttctat taaaccattt ttattcaatt aatacgcctt
      421 ttcctcgaat acgttgaact ccccaaataa agtcttctgt ttcttcagaa acgatctttt
      481 tgcgagctct tattttcact gtaattcggc caatataggc tccacttcca acccatcgat
      541 taatatggct tttcatgttc catgcaacga aaatatttcc cttgttgacc tgacaattcc
      601 cgccataaat ggagtcatta cagttaatct gtccagaaga agagtttacg tattgcccaa
      661 tagttgagaa atagaaggtt tcaaatataa cgcttaaaga atccatcgtt aacgtagaat
      721 cgccttctgc aatcaaatta cgaagatatt cttctaaatc aacatcaata atgtggccat
      781 gttttccata agtcctcatt aactcatctt tagttacatt cttttcaatc aaatgagtgg
      841 tgactgttgg agaattaatt aaacgatcat ctagaggatt tactgaaata agcgtagtag
      901 aggaaatctt agttttttgt tctcctacaa tacgtaccca tggatttaaa tcatgaggat
      961 gagctccact gagatcagta gcaattccag gattaaagcg aatactatct cctacggcag
     1021 gtatatctaa tccatcactt tccacaaaat acaggtcata gcgatgtagg tcgctagctg
     1081 tcgaagattg tggaatcatc ggtaattcat tcagatgccc atctcgccag taacggaatt
     1141 taaatagatc tcctaacaaa agataatcgt ctttttgaag agcttcacta aaggttaagg
     1201 agagaaccgt gacgttgccc gatttttctt ttacagtcgc agataaaata attgggccca
     1261 ccttatcttg tatttggcca gccatatcaa agataatctt gtcttcattg ccttcggggt
     1321 tataagtgaa tgaagttaaa gaaaaaccaa aataaggttc atccaataag ccagtgaaca
     1381 ctttatttgt aaatccatca ctgagaagaa caatagagga agtctcttcg aaagcagcct
     1441 gtaattcagt tttatttaca aaacgcatca aagaatctcc gtatcgccaa gaaagagaat
     1501 ctggaaattt acttccgata aggggttcag tatatttaag gtgaagacta tctggtattc
     1561 catctccatt gcgatcatgc atttctgcaa attcaagttg aggaacagga ggcactttta
     1621 aattaatatc tgtccaaagc aaaatattgg cataataatc gccaagaact tgaatgtaag
     1681 cgccattaat ttcttgggtt ccaagaatcc agaatgtgac ttgcccattg acgatagagc
     1741 tttcagtcaa aggatttcca agagaatcca taaattttag gttcggatca gaggagaata
     1801 gagaaatatt accatcgcag ccagcgcaat tctcttcaaa aatacgaacc cgtacagggt
     1861 aaagtgtatg agcccattct ccaatcacgg aagtatcggc agaaataatc tcccaatcat
     1921 cattagcaaa agcaatagaa ggccttgggt attctgctac tgtaaaccat acttcaccag
     1981 agtgagattg atctgcttgt aagttaaatc ttaaacaata ggtgccagga gcaagagatc
     2041 gattgattcg aattaagcta gtatcaattg taaaaccagc cattccagaa tcaattacaa
     2101 tacctccata gtgagcccct tcttgaagcc aaacaccttc taaaggcaga ttgcctccaa
     2161 ttagattaaa atcagatgga gcataagttt tttctttatc cttcattcca gagaaatcac
     2221 aagcgagaga ttgttctcga acaatttgcc aaatctcata cttaatgatc tcaggagatg
     2281 gagaaacatc ggtaggaaaa taggaacgtt cggttctaag atccattgag gttctcatca
     2341 tgaaattgga tttatatctt tttcgctctg catagaatat atggaaagga tatgtttctc
     2401 caggagttaa gccaagggta tctaaattga ctgaaccaaa gactttgttg tgctggccgc
     2461 caatatcgac aactaagcga ttattaataa aaacccaaac gtcatcatcc ccgttaaatt
     2521 caaagtattg accaggcaca tattcaaatt cagctactgc tttcattgtc attccaaaat
     2581 tatgatctcc accttcaccg ctagcagagt cgtattttgg attaggaata agaccttctg
     2641 ggtctaggaa tttaaaatca tccagtaaga aaaagccatg ttctggacta ctatcatctt
     2701 tttgtccaag ccaaagcccc tcctcatcaa gttcaagcgt taaatctcta catgtcgcgt
     2761 ttgtataaac ggttccattg actacagaaa gtgtttctgg taagaaccat ttattcaagt
     2821 tatttgcatt tgtgcatttc gtttcaggga aagaggcgct tctgacaggt acttcatttg
     2881 gacctaagaa taattccacc attccttgtg tggctccatt accgcctgga gtcccttctt
     2941 cacaaccatt agcaccaaaa tctgcatctg taccaattcc atattgtgga acaccttgcg
     3001 ccacatgtcc gtaaggccct tcttccatgg aaccatcata ccagtcaaac atcattacag
     3061 cgagagtttt gattgggcaa tctccaagaa tatttgggaa agaagaatgg aatgttggac
     3121 gacccgaagg ataaggagta ggtctaatcc aaatggtatc agcgatagaa aaaatactgt
     3181 ctaaagtcat tgggaaacca tctgtacgcc ctgcatcgga gtaaatttca gtaccgatgg
     3241 attgcttaaa gagaagatgg aaatcgtcta tagaaccttg gtaagttgct tcaaaccacc
     3301 cgcaataatt tttaacggtt ttcattccaa gagtatctcc gttaacaatc atttgaggag
     3361 acgtgttaga ccaaggattt aagattcgaa ttcttttttg atagatagga gtattggtga
     3421 taacaatacg atcatcgctt tcaattctaa accaagcgtc ataaacccct ttgggaaata
     3481 tttcacttat ggtttgagtg gttgaaaatg tgcttgttgt gttatcggca taatctcgca
     3541 tattaaaaac aacaaaatca ctcccgcttc ccacgttttc tgcatcgtgc aagtaccagt
     3601 tatccatatt actttgctta tggatggttg tgttagaaat agtggtaccg acatttattt
     3661 gaatttcact gttacctgtc caataaagat ggaaactata gtcgccatta aaacattcgc
     3721 ccttacttgt acggactgta tcaagcctta cttcagaggc tgtaccatct atgaaaaccg
     3781 ttttcccttc agaaattaaa ggcccaatat cgatggtgct ttgtgaagca ggaaaggtga
     3841 atgaggagtg tggtctttga aaataaacag gtataaaaac ggatggagat tgaaaggtat
     3901 atgaaaacca gccacaataa tcacttcctt ctacagcagg ctgaagagga atcgtatcat
     3961 taccaaaaat catgttcggt atgattttat taccccaagg gcttttaaac caaatagacc
     4021 atgggccaga gggctttacc gaataatttc catcttgatc aatataaacc caaacttcat
     4081 ttccaaaaac ggtgaaatcg cgtaagcgag gtgtttcact ccatgtttat tcaccagaag
     4141 agagttcgat tgttgtggta gaatactcag ttacctcggc aacagtaatt tcaacagtat
     4201 gccaataatt cccattagcg gtcatggcat aagttgtgtt ttctgtaaaa cgtcctgctt
     4261 taaaagaatg aggatcgtca gaagcccaag gcgactgaat atgaagaata tatggctgcg
     4321 ccattaaggc gttcgcaaaa aatagtacca ataaataaac acaagaaata atattcttgt
     4381 tcatccttac cgtccttatc gtcgttgata tcccaaaatc aacgaaaaaa gtgagggttg
     4441 ctgaattcaa cactccttac acactcccct aatataaatt tatatagcca ttatggaata
     4501 aaggcttttt tagaaagagc aagatttata cataaaaatc ataaaaacca acaaagagaa
     4561 tgtaaaatgt ttttaacatt gcgaaagcaa agcaaaaaag ccatatataa tctattttga
     4621 agttattgaa tttgagtttt agatggaaat aaaatgagaa aagtatacgt aatagcgcca
     4681 actggaatta atgctgaaac ggcaaaaact gcacattctt tttatgatta tattgtaaaa
     4741 caagagccaa aaactgttta ttttaaaccc atcggtgagt ctcatggatc catttcgcaa
     4801 gagagagtcc gtgttctttt atcttcgggt aattcaagtg ttttaatgga acaattactt
     4861 gctcttttta tggaagtggc gagtgatcaa gaggtcattg ttgtagaagg tgtttctgca
     4921 aatgagtttc catttagaag taaaaaatta aatgaaatta ttgctcaagc tttagatgct
     4981 tctgtgatct tagtctcttc agctacaggg aaaacgccat tagccgtttg cagcgaagta
     5041 gaaatagaag cacaagatta ctttgaaatc aaggtgagag ttgcgtgtag cgtcattact
     5101 ggagtagaag agtctgcaaa agaatctttg agtcaagctt ttaaaaatag cttcgttaat
     5161 gttttagctt atgaaatcaa taacgagatg aaaatagatg aatctcttgt taaaaaaatt
     5221 atcgagttaa aaacagaaca tcatattacg caacctgaat ttagagcgaa tttgattcgt
     5281 caagctggtt taaatctaaa acgtattgtg ttaccagaag gaacagaacc gcgtacagta
     5341 aaagcagcta ttcttgccta cgaaaggaaa atagcgcttc ccgtattgtt aggaaatcgc
     5401 actgagatag aatacatcgc agaaaaacat gggcttgatg tacctgcagg cctagaaatc
     5461 attgaagtca atgaaactac gggtgagcga tttgtaaaca ctttagtaga attacgaaaa
     5521 gaaaagggaa tgacagaaga acttgcccga gatttattaa aagattctgt ttggattgga
     5581 acgatgatgc taaaaatggg agaagtggat ggtcttgtca gtggggcagt acactccaca
     5641 gcagatactg ttcgtcctgc attacaagtg attaaaacag ctcccggagt taaatctatt
     5701 agttctgtct ttttcatttg tttgccttcg cagattttag tttatggcga ttgtgctatt
     5761 aatcaaaatc cagattctag tgaacttgca agtattgcta ttcaatgtca tgatacggcg
     5821 aaggctttcc atttaccagc aaaagtcgca atgctcagtt atagcacagg tacatcgggt
     5881 aaaggtgatg atgtggattt agtagtagaa gccactcgct tagtaaaatc cgttcgtcca
     5941 gacattgtta tcgatggacc tttacagtac gattctgcaa ctgctgaaag tgtagcaaag
     6001 attaaggctc cacatagtcc tgtagctggt aaagcaaatg ttcttgtatt tcctaattta
     6061 aatgcaggta atattactta taaggcagtg caacgttcag cagctaatgt gatttgtata
     6121 ggccctatgc ttcaaggttt agcaaagcct gtgaatgacc tttctcgcgg tgcttttgta
     6181 gatgatatta tttatacgat tgctcttaca gcgattcagg ctcaatcgat agattaacgg
     6241 cgcttcttat ttcggacttt tttttgtagc tctttaagat gccatcggcc tcttttgtct
     6301 tctaagaaaa agtctgttcg gacgcctaga tcaaggcctg cttggatgat tttttctgcc
     6361 tcatcgtata agccttgatc aaatcgtaat tctgagagaa aaaaatagtt ataggggtct
     6421 ttggaatctt gttctaaaga catctttaaa tatttctctg ctagtttttt atctggccaa
     6481 gagagtataa taggaatgta gggaagtatt tgatgagaac gaccaagaat ttgatagtct
     6541 tgaatagaat cagctaaatc tcttactttt ttagctacgc cttgtttgac tgcaatcaga
     6601 ggatttgttt ctgctcccca catagataaa atgactatat agattttggt tatgtttctg
     6661 ttattgggat atagggagtg tattttttca cttaaaagag tcacttcgtt aagtacgtct
     6721 ttcctcttat ctttttcagg gctagcaaag cgtagcatga aataataaga ctttaacagc
     6781 ccctcggcag ccttttcctg aatgagcgta tctgcagctg caaaagctct ttcataagcc
     6841 tggatcattt tctttacatt atttgtatcg gctttatcgc cattcgcttt ttcagcacgt
     6901 aaagaataac acgaatcccc gattctaagg tcttcgtttg caactcctat accccaaacc
     6961 caaaggagta gaatagtgag agccctaaaa accattaaaa aagattaaac tttccaggtt
     7021 tgcagtaatt tattcacctg ggctatacga gccttttctt cagctgttcc gtgaaacgct
     7081 ttgaaaagaa ggttccggta ttccatcagt gctgtgcgaa tgagtgtttt ttcttctttt
     7141 tttaacatac tggctccaaa tctaaacaat ctgtgaaaaa aatcaacgtt taataaaatt
     7201 acttttttct gttgtgacgt ttaccagaaa aatagtcttg ttgtcttgta gcacctaata
     7261 ctgcatgcca aagagcatta ttaggctcta tttttcgtct ttcacttaca gcatagtcta
     7321 tagggacatg tgtgaattga ttattcatac ttcctacaac catattcgtt tttccagcca
     7381 ttgccgcgtg aactgcattt tctgctaatt ggaaacaaaa aatggcgtct gttcctttag
     7441 ctggaatgct tcgaacagtg tagcttgggt caaaatactt aatgttaatt tctttgccaa
     7501 tactcttaaa atgagcttct attttttcag ttaaaaaatc accgatatca tttttcaaga
     7561 tattgccgct ggcgtcacga cgttcaggaa tgttcttaaa taggtgttga cccgcacctt
     7621 cagcaacaac catgaccgca tgtgttttac caatcgaata acggttttct agtgcatgta
     7681 gtaaaccatt ttcaccctct agtacaaaag gaacctctgg gatcaaacaa aaattcacaa
     7741 cagtcgttgc taatgaagca taagcagcga taaacccgga atcgcgtccc attagtttta
     7801 caagaccaag cccattaaat acaccgttgg cttcattatg ggcgcttgta ataacgtctg
     7861 tagcgcttaa gacagctgtt tcaaaaccaa aagtacggtc tactaaattc aagtcgttat
     7921 caatcgtttt aggaattcca attacagaaa tgggttgttt gcgtcttttt atttcagctg
     7981 caatttcacg agctccacga agagtaccat ctcctccgat acaaaagagc atatttatgt
     8041 taagtctcat taaagtatcg accataacag aaacatcttg ttcgcctcga gaactaccca
     8101 atatagttcc accatcatct tgaatggcgt ctactacttc tggcgttaac acaactggag
     8161 aaagaccata agaagggttc aatccgcgat agccataagg aataccaaaa atattttgga
     8221 cgccataatc aaaccaaagt acttggacaa gacctttgat gacgttattt aatccagggc
     8281 agaggccacc acaagtaaca atagctgcac gggtccaagc tggatcgtga tagatcattt
     8341 ctctaggccc tgcggcttct aaagaaggaa gatgacttcc tgtggcaaga agagattcaa
     8401 ttttttgcac gtctgtacaa aggctgacgc gttctgtatt gctgatgaat cgcataccca
     8461 tcattgggga atgaagagta ggagtaccta ctgtttgaat agataaatca tattgatctg
     8521 gatcagaaat aatatcgtag agcgtcatat aaaatccttt tgggtcttta taaaaatcta
     8581 ataaaggagt agctcaaagc atttaacgag ataaaagtaa caaaaactat atcttgcggc
     8641 aaagtgtgct gcagtaatgc ggagtgtatc tggaaaataa aactgctttt agatcgaaat
     8701 cggaggaaaa tgaatttttg tgcgtaatcc agggctcgcg ttttcaattt caattctcat
     8761 gtttgtgaga gagcaaagct ttttgaccat ggaaagccct attcctgtgc catttgtttt
     8821 acgagtcatc tcattttcaa cgcgataaaa ttcttcaaat actttggtca attcagagcc
     8881 tggaattcca ggaccatagt ctcgaaccac aaaaaagatg tcgttaccat cgactcttaa
     8941 ctcaagaacg atcattttgt at
//
LOCUS       DAOOBB010000069         8776 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_9832_length_8776_cov_4.693154, whole genome shotgun sequence.
ACCESSION   DAOOBB010000069 DAOOBB010000000
VERSION     DAOOBB010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 8776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8776
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9832_length_8776_cov_4.693154"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1834)
                     /locus_tag="PLT31_04555"
     CDS             complement(<1..1834)
                     /locus_tag="PLT31_04555"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-95 repeat protein"
                     /protein_id="HPW94443.1"
                     /translation="MNSVKTSITALLVSIGLFFAVSGTAQEAQQPAAKAQENHAPAVN
                     GIPGESISEGGSFATIKLDQYVSDEEDKPASIRWTVSGAKDLKVTISDRIATIKTPDA
                     YWNGSEDITFVATDTKNASGSETVTFTVESVNNPPELKTIPDQTIDEGKKFTVIKLDD
                     FVTDKDHPKDQIIWETDIQAVGKEQGIGDLSIEIDKNRMANVIIPDTNWYGAAKITFI
                     ATDGEYASAKTTASFTVRSINDAPIVQKAPDQTINEKNQFEQINLSDLVSDIDDDINT
                     IKWSVSGGNQLKASIDKSNIASISIPNENWNGPTETFTFTATDSKGAAASFKTSFTVK
                     SVNDAPEFISGITDQIIDEKQQFKSIILDKLVKDIDHSFEDLKWSVSGNKDLKVQIAG
                     KEAKLIIPQNLWNGSETITFKVTDPAGAFAESQATFTVNSINDAPVFVKAVPNQFIDE
                     KKKFVSINLDEFLKDPDHKNADLSIEATVKHQGKEPESGTLNVSVNEKHVANIEIPDT
                     YWNGAAIVTFTVTDPESATAKQDVTFTVKSINDLPVFKKIPDQTVEEKSELSSIVLDE
                     FLSDADHDISKLNIELSGLKEIKATLNAKTREVSFKTPNINWNGS"
     gene            2182..2949
                     /locus_tag="PLT31_04560"
     CDS             2182..2949
                     /locus_tag="PLT31_04560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPW94444.1"
                     /translation="MEKIDDNLKRFLKEVRGTQVLSRSEEILLFQLMAQGNREAKRKL
                     IVANIKFVTKVALQYKSSSLSSADLINEGTMGLIRAIESYDPSRGLKFISYAVWWIRA
                     YITRAIYEQDTLIRLPSNQRWRLNQARRSNEKVDDLEENVKVLMQMGLSGVSIDSPIS
                     NATTVTFADVIASPHKSTDEESDALISVLKKELTGREYQVMVDFYGLNDASSKTLGEI
                     AKTLELSRERVRQLRNQALQRIRKTMNRSESSCRSFL"
     gene            3024..3434
                     /locus_tag="PLT31_04565"
     CDS             3024..3434
                     /locus_tag="PLT31_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94445.1"
                     /translation="MKFVFLCDANYLKGDLVSFVNNFPTNHELVTMTAEEFLQTKGFL
                     EGTFAILAERSTWQKNFSLFRYFGLLPVLEVLPLGVVSRSRRIEPLKGRSSVRGSEVY
                     FNPSASPEELFMAIDKFISAPPFGFVYSRGSAKA"
     gene            3440..4945
                     /locus_tag="PLT31_04570"
     CDS             3440..4945
                     /locus_tag="PLT31_04570"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPW94446.1"
                     /translation="MRRRVLESLASQGALVAFVSAPVDFLEASEAILLARSFKTSALS
                     LVIDFQGIFKSYSVRSCGSLFSSRLQFNESIEEQDGFALLTSNGISIPEDPQSIRLFL
                     QNLVSLQKNHPFILLLLPAERSPFMEKILSAANLIVVSASEEKSQMMVSQAISSIDLM
                     KPGKLLWLDSNLPSSKVYSRLYKEAKFYSGIKQKAMDAWKSDFSTFAAEIKTILKIQI
                     LKKNPLDGLARFFLGLWPFLFLIALVLPFIIPHKIEPGISNLRDARQERNALSGAPYF
                     LFEFDGKETLQRIGRYAIGRFNAQVTTEKILNQYVEEVLVKNGYSKSSWKKEKVNIPP
                     AGTRLQFLPPDFLSPSKNDSLGLAWNFLTSLISDSVAYITELYHESPKAVGRIHKGVD
                     IASRHGARILAPFAAKAWTFKDDRGGVVIGLVHEKKVMLFMHCDQLLYLDGQEVMAGD
                     PIATVGTTGHTTGPHVHLETGVIHPKGNKFLGNNRYKTVNPITWFYSQVKK"
     gene            5049..5486
                     /locus_tag="PLT31_04575"
     CDS             5049..5486
                     /locus_tag="PLT31_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94447.1"
                     /translation="MQLRFFLFASIATLFLGCSSISMPKARVVEHDAERTIPAIDEMI
                     VEMKQDYITKCYAPVVKRDPPENSCQTELFQMLERRYHTNYSQNHVSMASNDLFFRDV
                     EVQLKKMVRTDPDVRNAVKNGAFRSADEMLSYYKGKYAFELNQ"
     gene            5610..7016
                     /gene="leuC"
                     /locus_tag="PLT31_04580"
     CDS             5610..7016
                     /gene="leuC"
                     /locus_tag="PLT31_04580"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HPW94448.1"
                     /translation="MGKNLYQKVFESHTVAKLPSGQFQLFIGLHLCHEVTSPQAFAML
                     REEGQKVLFPERTFATVDHIIPTTAADRVRPLKDAISEEMFSHIEKNTKDFGIRFFGP
                     STEEQGVIHIVGPEEGVTQPGMTVACGDSHTATHGAFGAIAFGIGTSQVADVLATQTL
                     AMSPLKTRRLVFTGKLNKGVTAKDVALAYIAKLGVNGGVGYAYEFAGPVIEAMDMEGR
                     MTICNMAIEGGARVGYCNPDEKTFAFLKGKPYAPKADKWDAAIAYWKSVASDPDASFD
                     DEIEINCDDLEPMVTWGITPAQAIQLNENMPKVNDFSDSERKVVAEAYEYMGFEEGSK
                     ILGTSIDIAFVGSCTNGRLSDLQAAADIIKGRKVASSVKMWVVPGSMKIKKEAEALGL
                     DKIFTEAGAEWREAGCSLCLAMNPDKLQGRQISASSSNRNFKGRQGSPAGRTLLMSPA
                     MVAAAAIEGKVTDVRKYL"
     gene            7027..7629
                     /locus_tag="PLT31_04585"
     CDS             7027..7629
                     /locus_tag="PLT31_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HPW94449.1"
                     /translation="MSMPIINVIKGSGIPLRGNDIDTDRIIPARFLKCVTFDGLGDFA
                     FADDIKGLEAQGKTHPFRDPIYAKGSILVSNQNFGCGSSREHAPQALKRWGVRAIIAE
                     SYSEIFFGNCVALGIPCYKVDHATAEKILAWIEANPSTEMETSTQEKTLKLSNETIPL
                     ILADGPRGQFLDGSWHARAALMANMDKVAQVASNLPYMKF"
     gene            7834..8484
                     /locus_tag="PLT31_04590"
     CDS             7834..8484
                     /locus_tag="PLT31_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94450.1"
                     /translation="MKKNVLLVLFTLSFLFFTGCASSKQVIDPSWVAKPSTVTVLIAE
                     PYVINIDDLVDDLPNELQTFPHWFSGKLGVAYSNLSRVKTNVRVMTEKDFILDKATLS
                     NGKNIVVPRPKEVVDAELILCISPIRTSRFTVETTSSMPNGTNANQFNGTATTTSEYL
                     RYEAYYAYYDGKKGTNLGYGIVDVRSGFSFYMDASNWEENITNMVKNLIKGTPLAK"
BASE COUNT         2563 a         1446 c         1761 g         3006 t
ORIGIN      
        1 cagaaccatt ccaattaata tttggtgttt tgaaagaaac ttcacgagtt tttgcgttta
       61 atgtagcctt aatttctttg aggccagata attcaatatt tagttttgaa atatcgtggt
      121 ctgcgtcaga taaaaattca tcaagtacta ttgagcttag ctcgctcttt tcttctacag
      181 tttgatcagg aatttttttg aatacaggta aatcattaat cgatttaaca gtgaaggtga
      241 cgtcttgttt agcagtagcg ctttctggat cagtgactgt aaatgttaca atagcggctc
      301 cattccagta ggtgtctgga atttcaatat ttgctacatg tttttcattt acactaacgt
      361 ttaacgtacc agattctggt tcttttcctt ggtgttttac agttgcttca atagatagat
      421 ctgcgttttt atgatctgga tcttttaaga attcatccag gttaatagaa acgaattttt
      481 tcttttcatc aatgaattga ttaggaactg ctttaacaaa aacaggagca tcattaattg
      541 agtttacagt aaaggtcgct tgagattcag caaaagctcc tgcagggtcc gttactttga
      601 acgtaatagt ttctgagcca ttccaaaggt tttgaggaat aattaatttt gcttctttac
      661 cagcgatttg aactttaaga tctttatttc cacttacact ccattttaaa tcttcaaaag
      721 aatgatcaat gtcttttact aatttgtcaa gaataatgga cttgaactgt tgcttttcgt
      781 cgattatttg atctgtaatt cctgaaatga attcaggagc gtcattgact gatttaacgg
      841 taaaggatgt tttaaagctt gctgcagcac ctttagaatc agtagctgta aatgtaaatg
      901 tttctgtagg accattccaa ttttcatttg gaatgctgat ggaagcgatg tttgatttgt
      961 caatagaagc ttttaattga ttccctccgc taacgctcca tttgatggtg ttgatgtcat
     1021 catcaatgtc gcttactaaa tcagagaggt taatttgttc aaattgattc ttttcgttaa
     1081 tggtttggtc tggtgctttt tgaacgatcg gagcatcgtt gatggaacga actgtaaatg
     1141 atgctgttgt ttttgcacta gcgtattcac catcggttgc aatgaaagtg attttagctg
     1201 caccatacca gttggtatca ggaataatga cgtttgccat tctattttta tcaatctcaa
     1261 tggagaggtc tccaatgcct tgttctttgc ccacggcttg gatatctgtt tcccaaatga
     1321 tttgatcttt tgggtggtct ttatcagtta caaaatcatc taacttaata acggtaaatt
     1381 ttttaccttc atcaatcgtt tgatctggga ttgtttttag ttctggtgga ttgtttactg
     1441 attctacagt aaatgtaacc gtttcagaac cagaagcatt ttttgtatct gttgcaacaa
     1501 aagtgatatc ttcagagcca ttccagtaag cgtcaggcgt tttgattgtt gcgattcggt
     1561 cagaaatggt gaccttcaga tcttttgcac cagatacagt ccatcgtatg gatgcaggct
     1621 tatcttcttc atctgaaaca tattgatcta gtttgatggt tgcaaaagag ccgccttctg
     1681 aaatagattc acctggaatt ccattcacag caggtgcatg attttcttga gcttttgctg
     1741 ctggttgttg agcttcttga gcggtacctg aaaccgcaaa aaacaaccca atggatacta
     1801 acaaagcggt aatagacgtt ttcactgaat tcatttatat ctcccataag gccctaaaaa
     1861 caacattaac ccgaccacga aaaggttttg ttgagaaccc tacaaaataa aaaacaccgg
     1921 cactaggtgt ggccatgatg tgcaaaatat aacgtattta aagaaaacgt ggtgtttttt
     1981 ttcaaaaaaa agatattggt ttttttttgt ttacattaga aaaaaatcat aaatacctgt
     2041 aagttgttga aaaataagga gataagaaca ttttattttt tttattatcg caaaactttt
     2101 atttatctta aaaaaaactt aaaaaaacct tccgttttta taaaatgttg gtatttttgt
     2161 taaagtgagg actttgaaaa tatggaaaaa atagatgata acttaaaaag gtttctaaag
     2221 gaggtgagag gaactcaagt tctttcgcgc tctgaagaaa tacttctttt tcaattaatg
     2281 gctcaaggaa acagagaagc caagcgtaag ctgatagttg ccaatatcaa atttgtcact
     2341 aaggtagctt tacagtataa atcgtcgtct ctttcttcag cagacttgat taatgaagga
     2401 actatgggat taattcgagc gatcgaaagc tatgatcctt ctagggggct taagttcatt
     2461 tcttatgccg tttggtggat aagggcttat atcacaagag ctatttacga gcaggatact
     2521 ttaattcgat taccttcaaa tcagcgttgg cggttgaatc aagctaggcg atccaatgaa
     2581 aaagtggatg atcttgaaga aaatgtaaaa gttcttatgc agatggggtt atctggtgta
     2641 tcaatcgatt ctcctatttc aaacgcgaca acagtcactt tcgctgatgt gattgcatct
     2701 cctcataaat ctacagatga agaaagtgat gctttgattt cagtgctcaa aaaggagctt
     2761 actggcagag aatatcaagt gatggtcgat ttttatggat taaatgatgc ttcttccaag
     2821 acattgggtg aaatagcgaa aactcttgaa ctttcaagag aacgggttcg acagcttcga
     2881 aatcaggctc ttcaacgaat tcgaaaaaca atgaatcgtt cagaatcttc ttgtcgttct
     2941 tttttatagc tataaaaaat gacaaaatcg agtagataga ttatctttgt attcactgca
     3001 attctttaca gatgggtttt tttatgaaat ttgtgtttct ttgtgatgcg aattacctca
     3061 aaggcgattt agtttcattt gtgaataact ttcctaccaa tcatgaattg gtcacaatga
     3121 ctgctgaaga gtttcttcaa acaaaaggct ttttagaggg cacttttgcc attttggcag
     3181 aacgttcgac atggcagaaa aatttcagtc ttttccgtta ttttggttta cttccagtac
     3241 tcgaagtgct tcctcttggc gtggtcagtc gttcccgtcg aattgagcca ttaaaaggtc
     3301 gtagctctgt tcgtggttcg gaagtttatt tcaatccttc cgcatctcca gaagaactct
     3361 tcatggcgat tgataaattt atttctgctc ctccatttgg ctttgtgtac tctagaggtt
     3421 cagcgaaagc gtaaggatta tgcgacgtcg ggtactagag tctcttgcct ctcagggggc
     3481 tttagttgct ttcgtgtcgg ctcctgttga ttttttggag gcttcagaag caattctttt
     3541 agcgcgctct tttaaaacga gtgcgctttc tcttgttata gattttcaag ggatttttaa
     3601 atcttattct gtaagatcat gtgggtctct tttttcaagt cgtcttcaat ttaatgaaag
     3661 cattgaagaa caagatggct ttgcattgtt gacttctaat ggaatctcta taccagaaga
     3721 tcctcaatcg attcgtttgt tcttacaaaa tcttgtttct ctacaaaaaa atcatccttt
     3781 tattttgttg cttctgccag cagaaagatc tcctttcatg gaaaagattt tatctgctgc
     3841 caatttgatt gtggtgtctg cttcagaaga aaaatcgcaa atgatggtct cgcaagcgat
     3901 ttcatctatt gacctaatga aaccaggaaa actcctttgg cttgattcta atttaccaag
     3961 ttcaaaagtt tattctcgtt tgtataaaga ggctaagttc tattcaggca ttaaacagaa
     4021 agcgatggat gcttggaaat cagatttttc tactttcgct gctgaaataa aaacgattct
     4081 caaaattcag attttaaaaa agaatccttt agatggatta gctcgttttt tcttaggtct
     4141 ctggcctttt ttatttttaa ttgctcttgt tcttcctttt atcattccac ataaaataga
     4201 acctgggatt tctaatttaa gagatgctcg tcaagaacgc aatgctctat ctggtgctcc
     4261 ttatttttta tttgagtttg atggtaaaga aacgttacaa cgtataggcc gctatgctat
     4321 tgggcgattt aatgctcaag tcacaacgga aaaaatatta aatcagtatg ttgaagaagt
     4381 tcttgttaaa aatgggtatt ctaaatcttc atggaaaaaa gaaaaagtga atattcctcc
     4441 tgcaggcact cgtcttcagt ttttacctcc agatttttta tctccatcta aaaatgattc
     4501 tcttggttta gcttggaatt ttttaacaag tctcatttcg gatagtgttg cttatataac
     4561 agagttgtat cacgaatcac caaaggcagt tggacgaata cacaaaggtg ttgatatcgc
     4621 tagtcgtcat ggagctcgca ttctagcgcc ttttgctgca aaggcttgga cgtttaagga
     4681 cgatcgtggt ggggtagtga taggtcttgt tcatgaaaag aaggtgatgc tttttatgca
     4741 ttgcgatcaa ttgctctatt tagacggtca agaggtgatg gctggagatc ctatagcaac
     4801 agtgggtacg actggacata cgactggacc tcatgttcat ctagaaacgg gagtgattca
     4861 cccaaaggga aacaagtttt tggggaataa tcgttacaaa actgtgaacc ctatcacttg
     4921 gttttattcg caagtcaaaa aatagcttgt ggtaagaaaa aaattacata aaaagagtcg
     4981 tttttattaa atttcttcat aactttgttg ttgggataag gatggatggt ttatttatag
     5041 gagtatttat gcaattacgc tttttccttt tcgctagtat agccaccttg tttctcggat
     5101 gttcatccat ttccatgcct aaagcacgtg ttgttgaaca cgatgcagaa cgtacgattc
     5161 ctgcaatcga tgaaatgatt gtagagatga aacaagacta tatcactaaa tgttatgctc
     5221 ctgtcgtcaa gcgtgatcct cctgaaaatt cttgtcaaac agaattattt caaatgctag
     5281 aacgtcgtta tcataccaat tacagtcaaa atcatgtgag tatggcttct aatgacctct
     5341 tttttagaga tgttgaagtg caattgaaaa aaatggttcg caccgatcca gacgttcgaa
     5401 atgcagttaa aaatggtgct tttagaagtg ctgacgaaat gctttcctac tacaagggaa
     5461 agtatgcatt tgaattgaat caataaaact aaagtttcct tgttgttatg gcgaaaggct
     5521 ttttgattca aatcaagaag cctttcgttt ttttatatgg aacaatttta ctatatttgt
     5581 acaacctttt tttttgtaat ggtaatttta tgggtaaaaa tttatatcag aaagtatttg
     5641 aaagtcatac tgtagctaaa ttgccaagtg gtcaattcca gctttttatt gggcttcatc
     5701 tttgtcatga agtcactagt cctcaagctt ttgccatgct tcgcgaagaa ggtcaaaaag
     5761 ttttatttcc tgaacgcact tttgccactg tagatcatat tattccaacg actgcagcgg
     5821 atcgagttcg tccattaaaa gatgctattt cagaagagat gttctcccat attgaaaaga
     5881 acactaagga ttttggtatt cgcttttttg gtccttcgac ggaagaacaa ggcgtgattc
     5941 atattgtcgg tccagaagaa ggcgtcactc agcctggtat gactgtcgct tgtggtgatt
     6001 ctcatactgc aactcatggt gcttttggtg ctattgcctt tggtattgga acaagtcaag
     6061 tggcagacgt acttgctact caaacattag caatgagtcc tttaaaaacg cgtcgtttag
     6121 tatttacagg taaattaaat aaaggtgtaa cagctaaaga tgtagctctt gcttacatcg
     6181 caaagcttgg cgtgaatggc ggcgttggct atgcttatga atttgcaggc ccagtgattg
     6241 aagctatgga tatggaaggt cgtatgacta tttgtaatat ggctattgaa ggcggtgctc
     6301 gtgtgggtta ttgtaatcca gatgagaaaa catttgcctt tttgaaagga aagccatatg
     6361 ctcctaaggc ggacaaatgg gatgccgcga ttgcttactg gaagtctgta gcaagcgatc
     6421 cagatgcttc ttttgatgat gaaattgaaa tcaattgcga tgatttagaa cctatggtca
     6481 catggggtat tactccagcg caagcaattc aattgaatga aaacatgcct aaagttaatg
     6541 atttctcaga ttctgaacga aaagtggttg ctgaagcata cgaatacatg ggttttgaag
     6601 aaggatccaa gattttaggt acttcaatag atattgcttt tgttggtagc tgtactaatg
     6661 gacgcctttc ggatttacaa gcggctgccg atattattaa aggcagaaaa gtggcttcct
     6721 ctgtaaagat gtgggttgta cctggctcaa tgaaaatcaa aaaagaagca gaagcacttg
     6781 gtttagacaa aatctttact gaagctgggg cagaatggcg tgaagcgggt tgttcccttt
     6841 gtttagccat gaacccagat aaattacaag ggcgtcaaat tagtgcaagc tcaagtaacc
     6901 gtaattttaa gggacgtcaa ggaagtcctg ctggacgtac tttgttaatg agtcctgcaa
     6961 tggtggccgc tgctgctatt gaaggtaaag ttacagacgt tcgcaaatac ctttaaggag
     7021 aattttatga gtatgcctat tataaatgtg ataaaaggtt caggaattcc tttacgaggg
     7081 aatgacattg atacggatcg tattattcct gctcgttttt taaagtgcgt gacttttgat
     7141 ggtcttggtg attttgcttt tgctgatgat attaaaggtc tagaagctca aggtaaaaca
     7201 catccttttc gcgatcctat ttatgcgaaa ggctctattc tagtttctaa tcaaaacttc
     7261 ggttgcggtt ccagtcgtga gcatgctcct caggccttaa aacgttgggg agtgcgtgcc
     7321 attattgctg aaagttattc tgaaatcttt tttggaaact gtgttgcttt aggcattcca
     7381 tgttataaag tggatcatgc tacagcagaa aaaattctgg cttggattga agccaatcca
     7441 tcaacagaaa tggaaaccag cactcaagag aaaaccttaa aactttctaa tgaaacgatt
     7501 cctttaattc ttgctgatgg gcctagaggt caatttttag atggctcttg gcatgcacgc
     7561 gctgctttga tggctaatat ggataaggtc gcgcaagtag cttctaatct tccgtacatg
     7621 aagttttagt ttaaagctca ttttttattt aattaatagc tccttcaaag aaaaatgaag
     7681 gagctttttt gttttgaata tattttttaa tgctaaaaat aatcacttgt ttatatttta
     7741 ttggagcgct tcatattttt attttttatg gaaaaaggtc tgcaaaaaag aattctgtgt
     7801 ggggctagtt taatttttta aaggaaaatt ataatgaaaa aaaatgtttt gttagtctta
     7861 tttacactct cttttctttt ttttactggg tgtgcttctt caaaacaggt gattgatcct
     7921 tcttgggttg caaaaccgag tacagtaacg gttttaattg cagagcctta tgttattaat
     7981 atagatgatt tggttgatga ccttcctaat gagcttcaaa catttcctca ttggtttagt
     8041 ggaaaattag gtgtggctta ttccaattta agcagagtaa agactaatgt tcgtgtcatg
     8101 actgaaaaag attttatttt ggataaagcc accttgtcta atggtaagaa tattgtggtt
     8161 ccgaggccaa aagaagtggt tgatgcggaa ttgattttat gtattagccc tattagaact
     8221 agtcgattta cggtagaaac aacaagctca atgcctaatg gtacaaatgc taatcaattt
     8281 aatggtacag ctacaactac tagtgaatat ttaagatatg aagcttatta tgcatattat
     8341 gatgggaaaa aaggaactaa tttaggttat ggtattgtgg atgttcgtag tggtttttct
     8401 ttttatatgg atgcctctaa ttgggaagaa aatattacaa acatggttaa gaatttaatt
     8461 aaaggcactc cgttagcgaa gtaaatctct ggtcaaatca aaagatccta attgaatttc
     8521 acaaatagta ttttttgttt tttaagaatt tgtagtgttg agagaatgaa aacactttat
     8581 caattttaaa caggaaaaaa atatttttta tgaaaatagg ttttatttta ctataaagcc
     8641 tgtttttatt ttgttataag gcatgttatt ttcctccaca cacacaaaaa aaagaaactc
     8701 atcctttcgg ggatgagttt ctttttgtat ttcttcaaaa ccgagagcta ggctctcggt
     8761 ccaaaataag ccttct
//
LOCUS       DAOOBB010000070         8747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_9872_length_8747_cov_4.514036, whole genome shotgun sequence.
ACCESSION   DAOOBB010000070 DAOOBB010000000
VERSION     DAOOBB010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 8747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8747
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9872_length_8747_cov_4.514036"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..1431)
                     /locus_tag="PLT31_04595"
     CDS             complement(94..1431)
                     /locus_tag="PLT31_04595"
                     /inference="COORDINATES: protein motif:HMM:NF014113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 5"
                     /protein_id="HPW94451.1"
                     /translation="MNLSVSRTLLFCSSLLLLWGCGGDDKGLGSNDPFATYSSSITID
                     PFLSSAVIDPGLSSGIVDPGLSSPIIDPGLSSGVVDPGLSSPIIDPGLSSGIIDPGLS
                     SAIIDPGLSSATDPIVSSSSTISGPGIPVEAYPVANYGVILSNGKTGWSSRYWDACKP
                     HCSWISNGAEGQTDTTTEASYQANLTTARNCNIKDIEIPTFTLSRGFSEYWTGWEGTE
                     SACKSGKGGAFTCTDMAPVKVNDTLSYGFVAGPGSSTTCGKCFHLQFNGSFHDAGEHN
                     APKATHKALKGKHMVVMTSNIGHDVQTGQFDLMVPGGGVGQFDALSTMVNGSGVQWGA
                     QYGGFLTQCQKSLGYDNTLESYQNCVKDMCDAAFTGYDNLLRGCHWYADWYMAADNPT
                     YNWEEVECPQYLVDRYKTTINTTRRNIYAYQTDWSTYEGGEIPTDTRFPVDLE"
     gene            complement(1493..2908)
                     /locus_tag="PLT31_04600"
     CDS             complement(1493..2908)
                     /locus_tag="PLT31_04600"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HPW94452.1"
                     /translation="MAKKVQDALKDLVSLCKRRGFIFPGSEIYDGLANTWDYGPYGSE
                     LKRNIKDLWWRKFVASRPDVLGLDSSILLNPKVWQASGHVGNFSDPLIDCKACHERFR
                     ADHLLEEKLGEGCTVGKNFQDIAAMIVENQIECPSCGKKDFTEPRAFNLMFQTEIGVV
                     EGAGNKVYLRPETAQGIFINFRNIIDNVRPKMPFGIGQIGKSFRNEITPGNFIFRTRE
                     FEQMELEFFCEPGTEVEWYEYWKKFCMNWLLQDLGITAEKLRLREHAKEELSHYSNGT
                     SDVEYEFPFGWGELWGIASRTDFDLTQHQKESKVKQEYFDPITNKRYIPYVVEPSLGV
                     DRLLLVLLCDAYDVEKLDDGEERVVLHFHPSVAPVKAAVLPLVKKGKVKEVAESIFQN
                     LITRFNVEYDETQSIGKRYRRQDELGTPYCITVDFDTIGEGDDVDLSKKGKVTVRDRD
                     TMKQELVAIEDLEDWLAKRVR"
     gene            3211..4437
                     /locus_tag="PLT31_04605"
     CDS             3211..4437
                     /locus_tag="PLT31_04605"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HPW94453.1"
                     /translation="MYKTILIFITLLFSSLFANSTPNPVDSVLTKKSVSTNDSVSIQN
                     SISTKKSVLTKDSVSTKDSVSTKDSVSSTLSTSNDSTLTFVGVGDIMMGLNYPDESPV
                     LPPEDGALIFADVKSILQDATLTIGNLEGVLLDLGGTPKRCSNPKLCYAFRMPERYVN
                     HLVDAGFDLLSVANNHSGDFGPTGRETSQEVLKKAGLGFAGFEGMSEKYYLEKDGIRY
                     GFTAFSVNNGTLRVTDAAKAKQVISDLRDSSDIVIVSMHIGAEGKKFNRITRKTEHFV
                     GENRGNPYAFARLAIDAGADIVFGHGPHVARAIDLYKGKFIAYSLGNFATSTSVNITG
                     VSGYAPIIKIKTNKNGDFLEGQIYSAIQQGPDGARRPLLDTTGACISEIKKLTEKDIP
                     EAKIVIEKDGKIVLKQ"
     gene            4452..5495
                     /locus_tag="PLT31_04610"
     CDS             4452..5495
                     /locus_tag="PLT31_04610"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPW94454.1"
                     /translation="MIFRTINNFNDTTNNDTTKKGCISPLNRLWPFLLVIFGLFSSAF
                     AETELKKEVLHRQESPKAKNSLDQNSNNLKEQLTEGLSPSSKSKLSISEETEEESAED
                     ENEDSEETAEEVSDSEPESSLEDEVAVLPDSAVLPDTMLFYMNQASLPCEGNRVTSPY
                     GIRRYRMHKGIDIKVQKGDTIRAAFTGQVSRVSYERRGYGHYVYIEHPELGISKTVYA
                     HLSKKLVKVGQVVQAGEPIGLGGNSGRSTGSHLHFEIRIQNMAINPAIFFNFENHAAT
                     QDTLTLSMVEIKTELDAIEKELAKHRYHKIRPGDNLGKIARKYGTSINKICQLNGIKR
                     TTVLRIGRVLRCS"
     gene            complement(5507..6631)
                     /gene="thiH"
                     /locus_tag="PLT31_04615"
     CDS             complement(5507..6631)
                     /gene="thiH"
                     /locus_tag="PLT31_04615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HPW94455.1"
                     /translation="MTFNEYHAQYDWNQIQEQIESKTINDVDRALQKKGQLNIDDFAA
                     LISKAAAERLEVMAQRSRALTLKRFGRTMQMYLPLYLSNECSNCCTYCGFSHLNKDLK
                     RLTLTDEQIISEINVIKKWPYEHILLLTGEDAKNSGFEYLRHAIQICKRYFKHISIEV
                     QPMDTEEYRILGEEGVSAVMVYQETYHRENYKKYHPSGKKSIFSYRIDTPDRICEAGI
                     RKVGLGCLMGLEDWRTDCFFTAMHLRYLEKKYWRTRYSISLPRLRPHAGLQDQKNIQT
                     DRELLQLICAYRLFDPDVEISLSTRESAIFRDNASKIAVTSLSAGSRTDPGGYSLSKE
                     ELPQFIVNDDRSPEEVAAMLMKEGYEPVWKDWDPVLDGNF"
     gene            complement(6636..7406)
                     /locus_tag="PLT31_04620"
     CDS             complement(6636..7406)
                     /locus_tag="PLT31_04620"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF017501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HPW94456.1"
                     /translation="MSDLIIADKTFHSRLFLGTGKFSSAQAMEAAIQSSQTEMVTVAL
                     KRVDLSAGKDPILFSLKSFQGQLLPNTSGARDAKEAVFAAKMAREALGTSWLKLEIHP
                     DPRWLLPDSIETLKAAEILVKEGFIVLPYIQADPVICKRLEEVGCAAVMPLGAPIGSN
                     QGLKTKAMLEIIIEQSQVPVVIDAGLGAPSHACEAMEMGADACLVNTALAVAGNPAIM
                     GNCFKMAVEAGRAAYESGLPAADYQQAIASSPLTSFLD"
     regulatory      complement(7479..7579)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(8009..8641)
                     /gene="thiE"
                     /locus_tag="PLT31_04625"
     CDS             complement(8009..8641)
                     /gene="thiE"
                     /locus_tag="PLT31_04625"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HPW94457.1"
                     /translation="MKKIHPLQILTWDDAPTSHLSQVKALLEAKAEWIQLRQKSGSFE
                     EKLKVAKEVVALCNQKQTTLIINDDPFLCLESGAHGVHLGLSDLPIKEARLILGPEAI
                     IGGTANTPEQVVQRMEDHADYVGLGPFRSTETKKKLSPVLGIDGVKAIASLVQSRKEK
                     GLYTCPFVVIGGITPLDLPLIKSAGANGVAICSAIVSAPSISDAVKQFSW"
BASE COUNT         2730 a         1781 c         1643 g         2593 t
ORIGIN      
        1 aacgtttctg aagattaatt cttcttttta taaatattaa aagcccctta ttatataata
       61 agaggctttt tttgtttgtt ttgaaaaagg gttttattca agatctactg gaaaacgtgt
      121 atcggtaggg atttctcccc cttcataagt agaccagtct gtttgataag cgtaaatatt
      181 tcttcttgtt gtattgattg ttgttttata tctatcgact aagtattgag ggcattcgac
      241 ttcttcccaa ttataagttg gattatctgc agccatatac caatcggcat accaatgaca
      301 accacgtaat agattatcat accctgtaaa agcagcgtca cacatatctt ttacgcaatt
      361 ttgataactt tctaaagtat tatcataccc taaagatttt tggcattgag ttaaaaagcc
      421 gccatattga gcaccccatt gtactccaga gccattcacc attgttgaaa gagcatcaaa
      481 ttgaccgact ccaccgcctg gaaccattaa atcaaactgc ccagtttgca catcatgacc
      541 aatattagaa gtcatcacca ccatatgctt tcctttcaaa gctttatgtg tggctttagg
      601 tgcgttatgc tcgcccgcat catggaaact tccattaaat tgtaaatgga aacattttcc
      661 acaagttgta gaagatcctg ggccagcaac aaaaccataa gatagagtgt cattgacttt
      721 gacaggagcc atatcagtac aagtaaaagc cccgccctta cctgatttac aagcactttc
      781 tgtgccttcc caccctgtcc aatattcgct aaaaccgcga cttagtgtaa aagtagggat
      841 ttcaatatcc ttgatattac aattacgagc agtcgttaaa ttggcttggt aagaagcttc
      901 tgtagtagta tctgtttggc cttctgctcc attagaaatc caagaacagt gtggtttaca
      961 agcatcccaa tagcgagagc tccaacctgt tttaccgttg gaaagaatga ctccataatt
     1021 ggccactgga tatgcttcca ctggaatacc tgggccagaa atagtacttg agctagaaac
     1081 aataggatcc gtagcagaag ataagcctgg atcgataatt gcagaagaaa gccctgggtc
     1141 aattatacca gaagataagc ctggatcaat aattggggaa gaaagccctg gatcaacaac
     1201 accagaagat aaccctggat caataattgg ggaagaaagc cctgggtcaa caataccaga
     1261 agataaccct ggatcaataa ctgcagaaga aaggaatggg tctattgtaa tagaagaact
     1321 atatgttgcg aaaggatcat ttgatccaag acctttgtcg tcaccaccgc agccccataa
     1381 tagaagtaaa gagctacaaa ataatagagt tcttgaaacg gataaattca tgtaaatctc
     1441 attttatttc taaaaaaagc ctctaaaaat cgaaaattgg ctaaatctgg tgttatctaa
     1501 cacgcttggc aagccaatct tccaaatctt caatagccac tagttcttgc ttcatggtat
     1561 cacgatcgcg aacagtgact tttccttttt tagataaatc aacatcgtcc ccttctccaa
     1621 tggtatcaaa atccactgtg atacagtaag gggtacctaa ttcatcttgc ctgcggtagc
     1681 gttttccaat agactgtgtt tcgtcgtatt ctacgttaaa acgagtaatg agattttgga
     1741 aaatggattc agcaacttct tttactttgc cttttttaac taaaggtaaa acagcagctt
     1801 ttacaggagc aacggatgga tggaaatgta ataccacacg ttcttcccca tcatcgagtt
     1861 tttctacatc ataagcatcg caaagtaaaa cgagcaataa gcgatcaaca cccaatgaag
     1921 gttcgacaac ataaggaatg tagcgtttgt tagtgattgg atcaaaatac tcttgcttga
     1981 cttttgattc tttttgatgt tgagttaaat caaaatcagt acggctagca atgccccata
     2041 gttcacccca accaaatggg aattcatact ccacatcaga agttccgttg gaataatgag
     2101 ataattcttc ttttgcatgt tcgcgcaaac gaagtttttc agcagtaatc cctaaatctt
     2161 gcaaaagcca gttcatgcaa aatttcttcc agtactcata ccactcgact tctgtacctg
     2221 gttcgcagaa gaattccagt tccatttgtt caaattcacg tgtacggaag ataaagttac
     2281 ctggtgtaat ttcattacgg aacgattttc caatttgacc tatgccaaat ggcatttttg
     2341 gacgcacgtt atcgataatg tttctgaagt taatgaaaat accttgagcc gtttctggac
     2401 gaagataaac tttgtttcca gctccttcca caacaccaat ttctgtttgg aacattaaat
     2461 taaaggcacg aggttctgta aagtcttttt ttccgcaact tgggcattct atttgatttt
     2521 caacaatcat agctgcaata tcttgaaaat tcttacctac agtgcaacct tcaccgagtt
     2581 tttcttcaag taaatggtcg gcacggaatc tttcatgaca agccttacag tcaattaaag
     2641 gatcggaaaa attgccaacg tggccagaag cttgccaaac ttttggattt aacaagatgg
     2701 aactatctag accaagaacg tctggacgac tcgctacaaa ctttctccac caaagatctt
     2761 tgatgtttct ttttaattct gagccgtatg gaccataatc ccaagtattg gcaaggccat
     2821 cataaatttc tgaccctggg aaaataaatc ctcgacgttt acacaaagaa actaaatctt
     2881 ttagggcatc ttgtactttt ttggccattt tgaatccttt taataaaaat cagcaaaaat
     2941 tatagttcaa atatagcaaa taacctataa aggtttagtc ctttttcatt ttttgattat
     3001 ctttaagaaa ccctgaaaat ggattgacga aatcaagcaa atcaaatgca gaaacataaa
     3061 ataagcttac aacaaaacac tgcatttaac gccaaaaata caagtgattc gaatcaattc
     3121 attgaaaata aattttaacg gaacagataa ctcgtttaga tttattttga ttagaacttt
     3181 tttattaaac cttttttatt aaacctgttt atgtataaaa cgattcttat atttattacg
     3241 cttctttttt cttctttatt tgctaattct actccaaatc cagtagattc tgttttaaca
     3301 aaaaagtctg tatcaacgaa cgattccgtt tctattcaaa attctatttc gacaaaaaag
     3361 tctgttttga caaaagattc tgtatctaca aaagattctg tgtctacaaa agattctgtg
     3421 tcttccactc tctcgacttc taacgactcc acgctcactt ttgttggtgt tggcgatatt
     3481 atgatgggct taaactaccc tgacgaaagc cctgttttac ctcccgaaga tggagccctg
     3541 atttttgccg acgtgaaatc cattttacaa gacgcgactc taactattgg aaacttagaa
     3601 ggagtacttc tcgatcttgg tggtactccc aaacgttgca gtaaccccaa actctgttac
     3661 gcttttcgga tgccagaacg ttatgtgaat catttagtcg atgctggttt tgatctttta
     3721 agtgtcgcca ataatcattc aggcgacttt ggccccactg gacgggaaac gagccaagaa
     3781 gttctgaaaa aagcagggct tggatttgct ggttttgaag gaatgtccga aaaatattat
     3841 ttggaaaaag atggcattcg ttacggtttt acggcttttt ctgtaaataa cggcacttta
     3901 cgcgttacag acgcagccaa agcgaaacag gttatttctg atcttagaga tagtagtgac
     3961 attgtaattg taagtatgca tattggagcg gaaggcaaaa aattcaatcg gatcacacgg
     4021 aaaacggaac attttgttgg tgaaaaccga ggtaatcctt acgcttttgc tcgcctagca
     4081 attgatgctg gagccgatat tgtctttggt cacggccctc atgtcgcccg cgccattgac
     4141 ttatataaag ggaaatttat tgcctatagt cttggaaatt tcgcgacttc tacaagtgtc
     4201 aatattactg gagtgagtgg ctatgctcct attattaaga ttaaaacaaa caaaaatggg
     4261 gactttttag aagggcaaat ttattctgct attcaacaag gccctgatgg agcaagacgc
     4321 ccacttttag atacgactgg agcttgtatt tcagaaatca aaaaattaac agaaaaagac
     4381 attcctgagg caaaaattgt cattgaaaag gatggaaaga ttgtattaaa acaataaggt
     4441 ttttataaac gatgattttt agaacaataa acaactttaa tgataccacc aataatgata
     4501 caactaaaaa aggttgtata tctcctttaa accgtttatg gccatttcta ttagtaattt
     4561 ttggtctttt ctcttctgct tttgcagaaa ccgagttaaa gaaagaagtt ttgcataggc
     4621 aagaatcccc taaagccaaa aactctctcg atcaaaactc caacaatctt aaagaacaat
     4681 taacagaagg cttaagccca tcctctaaat caaaactctc catttccgaa gaaacagaag
     4741 aagaaagtgc tgaagatgaa aacgaagata gcgaagaaac tgctgaagaa gtaagtgata
     4801 gtgaaccaga aagttcttta gaagatgaag tcgctgttct tcctgattcc gctgtattac
     4861 cagacaccat gttgttttat atgaaccaag cgagtctccc ctgcgaagga aaccgagtca
     4921 cttctcctta tgggattcgt cgttacagaa tgcacaaagg cattgatatc aaagtgcaaa
     4981 aaggagatac tattcgggcg gcattcactg gccaagtttc tcgtgtaagc tatgaacgtc
     5041 gaggctatgg ccattatgtc tatatagagc atccagaatt agggatttct aaaaccgttt
     5101 acgctcatct ttctaaaaaa ctcgtcaaag tgggccaagt agtacaagct ggagagccta
     5161 ttgggcttgg cggtaattca ggccgctcca ctggttctca cttgcatttt gaaattcgca
     5221 ttcagaatat ggcaataaac cctgctattt tctttaattt tgaaaatcat gcagcaactc
     5281 aagatacgtt aactctctct atggtcgaga tcaaaacaga actagatgcc atagaaaaag
     5341 aactcgccaa acaccgctat cataaaattc gtccaggcga taatttaggg aaaattgccc
     5401 gcaagtacgg cacgtctatc aataaaatct gtcaattaaa cggcataaaa agaacgaccg
     5461 ttctaagaat cggtcgtgtt ttaagatgct cctaaaagag gcggttctaa aaattaccgt
     5521 ctaatactgg atcccaatct ttccagactg gctcataccc ttctttcatt aacatggctg
     5581 cgacctcttc tggagatcga tcatcattca cgataaattg aggcaattct tctttagaaa
     5641 ggctatagcc tccagggtct gttcttgaac ctgccgaaag actggtgact gcaattttac
     5701 ttgcattgtc tctaaaaata gcactttctc ttgtgcttaa agaaatctct acatctggat
     5761 caaataaacg atacgcacaa atcaattgca gtaattcgcg atcggtttgg atgttttttt
     5821 gatcttgaag gcctgcatga gggcgaagcc ttggtaaaga aatggaataa cgggttctcc
     5881 aatatttctt ttctaaatag cgtaaatgca ttgccgtaaa aaagcaatcg gtacgccagt
     5941 cttctaaacc cattaagcag ccgagaccca ctttacgaat gccagcctca caaatacgat
     6001 ctggtgtatc gattctataa ctgaaaatgc tttttttgcc cgatgggtgg tattttttgt
     6061 aattctcacg gtggtacgtt tcttgataca ccatcaccgc agagacacct tcttctccta
     6121 gaatccggta ttcttcggta tccatcggct ggacttctat agaaatatgc ttgaaataac
     6181 gcttacaaat ttgaatcgca tggcgtaaat attcaaagcc agaattttta gcgtcttctc
     6241 cagttaaaag caaaatatgc tcataaggcc attttttaat cacattgatt tctgaaatga
     6301 tttgctcatc tgtgagagtt aatcgtttga ggtctttatt tagatggcta aacccacaat
     6361 aagtacaaca attggaacat tcattggaaa gatacagagg caaatacatc tgcatcgttc
     6421 gtccaaatcg ttttaaagtg agggctctac tacgctgcgc cataacctct aatctttcgg
     6481 ctgctgcttt tgaaattaac gccgcaaaat catctatatt aagctgccct tttttttgaa
     6541 gagcgcgatc gacatcgttt atggtttttg attcgatttg ttcttggatc tgattccaat
     6601 cgtattgtgc atgatattcg ttaaaagtca tgcttttaat ccaaaaatga tgttaatgga
     6661 cttgaagcaa tcgcctgttg ataatcagca gctggtagcc ccgattcata agccgctcgg
     6721 cccgcttcga cagccatttt aaaacagtta cccatgattg ctgggttgcc cgcgacagct
     6781 aatgcggtat ttacaaggca agcatctgcc cccatttcca tggcttcaca agcatgagat
     6841 ggagctccaa gaccagcatc aataacgaca ggaacttgag attgttcaat aataatttca
     6901 agcatggctt ttgtttttaa accttgatta ctcccaatag gagccccgag aggcatcact
     6961 gcggcacagc ccacttcttc aagccttttg caaatgacag gatctgcttg gatataaggc
     7021 aaaacgataa agccttcttt tactaagatt tcagctgctt ttaacgtttc aatggaatct
     7081 ggtaaaagcc aacgagggtc tgggtggatt tctaatttga gccaagaagt gccaagagct
     7141 tctcttgcca ttttggctgc aaaaacggct tcttttgcat ctctcgctcc agaagtgttg
     7201 ggtaataact gcccttgaaa tgactttaaa gagaatagga tagggtcttt tccagccgag
     7261 agatcgactc tttttaaggc cactgtgacc atttctgttt gagaagattg gatggccgct
     7321 tccatcgctt gagcggaact gaacttccct gtccctaaaa aaaggcggga atgaaacgtt
     7381 ttatcagcta taattaaatc agacataagt tcccttttgt aaaaaaacgc aatacgcctt
     7441 ttttacgata agcaggaaac gtacagagaa ccaaaatggc cctatctatg cgcttttcct
     7501 acggttcaat tacgaacaca ggttcaaggg gtttaatctc agctcttgcc gttcagcagg
     7561 ggcaccccca agcgaagctt tttgaagagg cttcttttaa aaataatagt aaataataaa
     7621 aaatcgggta gcttaaaaac gtgaaaagca cgtcggatat gataaaccgc gaaaagcgtg
     7681 ccgaatatta taaagcgcga aaagcacgtc ggatattata aagcgcgaaa agcgtgtcga
     7741 atattataaa gcgcgaaaag cgtgcctgat tatatatggc tgaataaaag cgcgaaatgc
     7801 gtgcctaaaa aagaaacgtt tgaagtaaaa ccgcgaaaag tgtgccgaaa acttaaagac
     7861 gtgcgaaata cacgtcggat attatatggc taatcaaaag tacgaaatgt ctgtctgaat
     7921 attaaagtga cgaataaaaa ctgcgaaatg cgtgccggaa aagagagatg cttgaaacaa
     7981 atgggcgaaa ctcatgccgg aaaagcgttt accaagaaaa ttgcttcacc gcatctgaaa
     8041 tggaaggagc cgaaacaatc gcactacaaa tcgcaactcc attggctcct gcagatttaa
     8101 ttaatggtaa atctaaagga gtaattccgc caataacgac aaaagggcat gtataaagac
     8161 ctttttcttt tcgagattgc accagagaag caattgcttt tacgccatca atccctaaaa
     8221 cggggctcaa ttttttttta gtctcggtag atcgaaatgg ccctaaaccg acataatcgg
     8281 catgatcttc cattctttgt accacttgtt cgggagtatt ggccgttcct ccaataatcg
     8341 cctctgggcc taaaattaga cgtgcttctt tgattggcaa atccgatagc cctaaatgca
     8401 ccccatgagc acccgattct aaacacaaaa aagggtcatc gttgatgatt aaagtagtct
     8461 gtttttgatt acaaagagcc accacttctt tagctacttt tagcttttct tcaaaagaac
     8521 ccgacttttg acgaagttga atccattcgg ccttagcctc taaaagtgct ttcacttgac
     8581 ttaggtgtga tgttggggca tcatcccaag ttaagatttg cagtgggtgt atttttttca
     8641 tgttcaagca atttaatatt tttttcatct aaaaacagtt attgttgacg gaaatggttt
     8701 tttcattaaa actcattaat gatatggtcg acggaaatgg ctattac
//
LOCUS       DAOOBB010000071         8717 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_9919_length_8717_cov_4.365620, whole genome shotgun sequence.
ACCESSION   DAOOBB010000071 DAOOBB010000000
VERSION     DAOOBB010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 8717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8717
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9919_length_8717_cov_4.365620"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            240..905
                     /locus_tag="PLT31_04630"
     CDS             240..905
                     /locus_tag="PLT31_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94458.1"
                     /translation="MTTLQARMDIRGFLRIQEHLATQLKIKNTPYADIFVNTTTQQIS
                     IIPTKKIKETSFRFIPSNSGFLIYLKGAMKLLDIPVVPGSVSLIQDKDKLIFSGKKKT
                     KKQGTWEIFPCRNSVGIPMISIDNRGTLILDKRCIELIDTKTNNTLTADFDAKKKMFS
                     LTFSKKGFINVRTIASHANISFMGTLSSFGLPIPQKRIRTGCSIQGKTLSFNASELIP
                     KKK"
     gene            918..1319
                     /locus_tag="PLT31_04635"
     CDS             918..1319
                     /locus_tag="PLT31_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94459.1"
                     /translation="MFDVLRLFYTVHYNVGAVCVLIVFGIIFVLSKKNYRTALILGII
                     LIALNVFVYQKTVNRVWTLTEGDRTWKFSVHQEWTITEIDEEKKDTTVHHWCWLDEWW
                     DQIAKTDLVGKLWGTKQVENYRKTSEERLKD"
     gene            complement(1316..2044)
                     /locus_tag="PLT31_04640"
     CDS             complement(1316..2044)
                     /locus_tag="PLT31_04640"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HPW94460.1"
                     /translation="MSRSVYFISDSHLGIIPQNSVPNREALFIETLLSWKEKASHIVI
                     IGDLFEFWYEYRHYVNKHHLKLYHALMNLTESGVQVHYLRGNHDFALESFFPEELGVQ
                     VHQELVLDIDGERFFCMHGDGIPRSDRGYRFLRRILDFPLNRFLFRLLHPDLGMEIAL
                     RVGSKSRQVSIDCPIVIEEYLEAAQKKMKKHQCKYFIHGHHHLKGIWNVPEGYVACCG
                     QWLFSLSYCVYNKESGLQIIEVSH"
     gene            2102..2650
                     /locus_tag="PLT31_04645"
     CDS             2102..2650
                     /locus_tag="PLT31_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94461.1"
                     /translation="MNRFIFISFSLFCFGCAPDFSGADIHIKENHPSPISLKSFSLKV
                     NDQSITPSPEIITAYNSSLSKADINADCEVHVSIQETINTEKESLWYLGVALMIPFWP
                     AMPMNTFLRLESTVEIMCQENLIHRLDFLEEEELDLFWYGPFRTYEIENRFEWFHEKM
                     IKRLENALQYESPVDAGCASDF"
     gene            2679..3365
                     /gene="rsmI"
                     /locus_tag="PLT31_04650"
     CDS             2679..3365
                     /gene="rsmI"
                     /locus_tag="PLT31_04650"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HPW94462.1"
                     /translation="MNTLYIVATPIGNLEDITYRAVRILKEVPLILAEDTRHSRLLLE
                     HYSISTPMESYHDFNKEKVTPKYIDYLKTKGDIALISDAGTPGIADPAFNLVRECVRE
                     NIEVRAIPGPSAMITALISCGMPTDRFRFENFSPKKSAQRLHLLERLKENADSTLIFY
                     VSPHHLVKFLEEIKTVFGEIPLALMRELTKKFEEHLIQTPSALLAHYKERTPKGEYVL
                     IFHPQGKGGL"
     gene            3362..5272
                     /gene="lnt"
                     /locus_tag="PLT31_04655"
     CDS             3362..5272
                     /gene="lnt"
                     /locus_tag="PLT31_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HPW94463.1"
                     /translation="MSLFSKVFLLTQIGLQALVAFLNPNTEGLYTYAPQILPFIGVIP
                     FLFFKKGRSSFIRGLYIFSIVSFAFLALDYTINGHIGIIQLAITFVPLFLLWLVYFVK
                     WNYRHLKSKDSLGALGLASVSWVLYALAFPPLPLGVGALIFLVPWFIVLQKKKPSVAL
                     FATFWSGVIYNAINYYWIYNVMKVGPAGFILLGLFLLIAYFSAYNVLAAFVFIQAQKV
                     KIKGHSILVWLFPFFYAGLEMTRTKGDFSFPWSHLGYALGNHLSLIQALSWIGIFGYT
                     ALIITSNILVTKAFTEKKYRYFIFTPVVIILCLWIQGTIVLSAKTAQPFYEDPSEKSP
                     MIAMVQPSIPQTKKWSKAYFDSITTKTWSIVDEFPTLHEVDLLVLAETAIPDILKRHP
                     DQIRHIRKVASENGLNLIVGALDYDKIKREGKAPLFKLYNSAFLFYPDSHNKKNERYI
                     KQHLVPFSERIPFDDVFPILNYVDFGEGDFTPGTETPVYKPFDWTPYICYEAIFGDQI
                     RRAIRNGSRIMVNITNDGWFGKSTAPGQHLNLIRYRAIENGYPVARNANSGISVFIDQ
                     YGHLDQQTGLFEEKIIARKMPLRTRDTLYTHIGDVVEIGLLVFFAIYLLYILIPLILD
                     KLRYRKNKKSER"
     gene            complement(5312..5953)
                     /locus_tag="PLT31_04660"
     CDS             complement(5312..5953)
                     /locus_tag="PLT31_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPW94464.1"
                     /translation="MSEKWFWLSDWAANMEIWEDDLVEASSESDHTFIAFEQLMKQPK
                     NLYTSIAGLKNADCVVAWGLGSLCLMLSAKDRPANQKWILLAPVYDFCDEDGGWSVRN
                     VEMLARQVTKAIKPTLESFLELMGPCDELIQEAWMETALKMNPETLASGLDFLCHNKI
                     EEPLENKGETIVYFGREDQLILPVLAKKISTILPSAKLLERPKSGHWPHTLLL"
     gene            complement(6097..7158)
                     /locus_tag="PLT31_04665"
     CDS             complement(6097..7158)
                     /locus_tag="PLT31_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW94465.1"
                     /translation="MINKKNLISKATCMKAIFFFISFLTVCAFAVQESDALLDRANAL
                     YQEGRYPQAILLYRKALARGADPVAVSFNTGNCLFQTEKYPEAAAAYRKAVDFSMGKF
                     SPALFNLASVYFRLKLYPESIATYHRALTLEPTNSSGWLFLGEAYTRTGDKVGTVKAL
                     QKAYQLDSIDITIVYQLSEAYIALEDFDLAIDLVRKAYTKNPEEIDFLVYLGDVYRLK
                     KEYDASAGAYREALVIRPDDVSLLYKLADVLAEGKQEYMAMDILSQALLIKPSFSDAA
                     IFLGNLAFDAKWWDRAEQAYEQAAKYNNKEAIYGFKNLAYEASLRKNNEEALQFLKKA
                     LQYYPEELTIQAEILNLEE"
     assembly_gap    7184..7283
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7284..7795)
                     /locus_tag="PLT31_04670"
     CDS             complement(<7284..7795)
                     /locus_tag="PLT31_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPW94466.1"
                     /translation="MKWIKRFLIFVAAVLSSFLLVFSVTLANYFIKGDFTRTNKYVKT
                     EIQIKKPEEVKKQIEKKKPTRKPSRQKSTSRSPKSGPRFSMDLNSVGGADGAAINDEL
                     VTGIRGGGFTKETGDVDEKPTSRGLPAFSPPQSIRDNEQDALLRLSFCVDVQGRVYDI
                     RVLEEMPVGKG"
     gene            complement(7806..8210)
                     /locus_tag="PLT31_04675"
     CDS             complement(7806..8210)
                     /locus_tag="PLT31_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPW94467.1"
                     /translation="MDFNIPRRKRKEMGIEMGPLMDIVFILLIFFVVTSSFTRETGVD
                     VTKPQAQSASQLEKENILIAITREGTIHIHERQVDMASLQDILKQTLAKTPDREAVVI
                     ADKGAVTGTLVQVIDACNLSGVKKVSIAAQSD"
     gene            complement(8211..>8717)
                     /locus_tag="PLT31_04680"
     CDS             complement(8211..>8717)
                     /locus_tag="PLT31_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPW94468.1"
                     /translation="FKNDLSKNVERMRVLLEKGDVDQAEQFLKSNMGIVARELFLALK
                     KGKENSAGFEDYISIRLLQTSRYIEQGTHVISMMAAAAPLLGLLGTVTGMVATFDVIT
                     LYGNQNPVLMADGISEALITTQSGLLVAFPLTLLKQRLDERIDMIRDQIELGATIIFN
                     FIEKRQEA"
BASE COUNT         2704 a         1699 c         1455 g         2759 t
ORIGIN      
        1 attcttctct tataattatc ttacaagacc gtaagaataa aataacgtta agcgaaaaag
       61 taagcaaaca atttcctgaa ataagaagag tttctcgtga tggaaaaact ttaaaacaag
      121 cgttgcaatt tatacttaat ttataacact ttataactta aaacagcaaa tgaatggttt
      181 atcattattt tttttactat tttgcaatta ttgattttta ttattagatg aggtctttca
      241 tgactacgtt acaagcaaga atggatatcc gtggattcct acgcattcaa gaacacttag
      301 ccacacagct taaaattaaa aatactcctt atgctgatat ttttgtgaat acaaccacac
      361 aacaaatctc cattattcct acaaaaaaaa taaaagaaac ttcctttcgt tttattccta
      421 gtaatagtgg tttcttaatt tacctaaaag gagccatgaa attgcttgat attccagtag
      481 ttccaggttc cgtttcttta attcaagata aagacaaatt aatctttagt ggtaaaaaga
      541 aaacaaaaaa acaaggtact tgggaaatat tcccatgtcg taactctgtt ggcatcccga
      601 tgatctctat tgataatcgc gggaccttaa ttttggataa acgctgcatc gaactcatcg
      661 ataccaaaac caataatacc ctcacggcag actttgatgc caagaaaaaa atgttcagtt
      721 taacgtttag caaaaaaggt tttattaacg ttagaaccat tgcaagtcat gccaacattt
      781 catttatggg cacattatcc tcttttggcc tccctattcc acaaaaaaga ataagaactg
      841 gttgttctat tcaaggaaaa acattatctt ttaatgcatc ggagttaatc cccaagaaaa
      901 aataaggacg ggaatccatg ttcgatgtac ttagactttt ttacacagtc cactacaatg
      961 taggggctgt ttgtgtttta atcgtttttg gtattatttt tgttctaagc aaaaaaaatt
     1021 atcgaactgc tttgattctt ggaattattc taattgccct taatgttttt gtttatcaaa
     1081 aaacggtaaa tcgcgtttgg acattaactg aaggcgatag aacctggaag ttctctgtgc
     1141 atcaagaatg gaccattaca gagattgatg aagaaaaaaa agatacgact gttcaccatt
     1201 ggtgttggct agatgaatgg tgggatcaaa ttgccaaaac cgatttagtt ggaaaacttt
     1261 ggggaacaaa gcaagtcgag aattaccgca aaacgtctga agaacgtttg aaagactaat
     1321 gcgaaacctc tataatttgg agccctgatt ccttattata aacgcaataa gagagcgaaa
     1381 agagccattg cccacagcag gccacatagc cctctggaac attccaaata cctttcaaat
     1441 gatgatgccc gtgtataaaa tacttacatt ggtgcttctt catctttttt tgagctgctt
     1501 ctaaatattc ttcaattaca atcgggcaat cgatactcac ttgcctgctt ttacttccca
     1561 cacgaagtgc gatctccata ccaaggtctg gatgcaaaag gcgaaataaa aaacgattta
     1621 atgggaaatc taaaattcgc cttaaaaaac gataccctct atctgaacga ggaataccat
     1681 ctccatgcat acaaaaaaat cgttcgccat caatatctag caccaattct tgatgcacct
     1741 gaacacctaa ttcttctgga aagaaagatt ctaatgcaaa atcatgatta ccacgtaaat
     1801 aatgaacttg aacgccagac tcagtcaaat tcattaaagc atgatacaac ttgagatggt
     1861 gtttattcac ataatgacga tattcatacc aaaattcaaa aagatcgcca atgatgacaa
     1921 tatgacttgc cttttctttc catgataaaa gagtctcaat gaacaaagcc tcacgattag
     1981 ggacgctatt ttgcggaata attcctaaat gggaatcaga gataaaataa acagatcgac
     2041 tcattgcctt taatttaata aagtaatgat catttgatga tttaatatta gttttaatcg
     2101 aatgaatcgt tttattttca tctctttttc tcttttttgt tttgggtgtg ccccagactt
     2161 ttctggagcc gatattcata ttaaagagaa ccatccctct ccaataagct taaagtcttt
     2221 ttctttgaaa gttaatgacc aatcgatcac tccctctcca gaaattatta ctgcttataa
     2281 ttcaagttta agcaaggcag atattaatgc tgactgtgaa gtccatgttt ctattcaaga
     2341 aacaatcaat acagaaaaag aaagcttatg gtatctaggt gtagccttaa tgattccttt
     2401 ttggccagcc atgcctatga atactttcct tcgattagaa agcacggtag aaatcatgtg
     2461 ccaagagaat ttaattcatc gtctagattt tttagaagaa gaagaattgg atcttttctg
     2521 gtatggcccc tttagaacct atgaaattga aaacaggttc gagtggtttc atgagaaaat
     2581 gataaaacgg ctagaaaatg cgctacaata tgaatcgcca gtcgatgccg gttgtgcttc
     2641 tgatttctaa tttatattct aaaaacagtt ataattttat gaatactctt tatattgtcg
     2701 cgactcctat tggaaacctt gaagatatca cttacagagc cgtaagaatt ctaaaagaag
     2761 ttccacttat tttggctgaa gatactcgtc actctcgcct acttctcgag cattatagca
     2821 tctctactcc catggaaagt taccatgatt tcaacaaaga aaaagtaacc cctaaatata
     2881 tagactatct aaagactaag ggagacattg ctttaattag tgatgcagga acaccaggaa
     2941 ttgcagaccc tgcttttaac ctggttcgtg aatgtgttcg tgaaaatata gaggtcaggg
     3001 caattcctgg accaagtgcc atgatcaccg cgttaatttc ttgtgggatg cctacagatc
     3061 gttttcgttt tgaaaatttt tcccctaaaa aaagtgcaca acgccttcat ctattagagc
     3121 gccttaaaga gaatgctgac tcgaccttaa ttttttatgt gagcccgcac catcttgtaa
     3181 aatttttgga agaaattaaa accgtttttg gtgaaatacc tctcgctctc atgcgtgagc
     3241 ttaccaaaaa atttgaagag catttaattc agacaccctc agccttatta gctcactata
     3301 aagaacgtac acctaaaggg gaatatgttt taattttcca tcctcaagga aagggtggat
     3361 tatgagttta ttttcaaaag tatttttatt gactcaaata gggcttcaag ctcttgtcgc
     3421 attcttaaat cctaataccg aagggctcta tacttatgcc cctcaaattt tgccttttat
     3481 tggggtcatt ccgtttttat ttttcaaaaa aggacgatcc tctttcattc gaggcctgta
     3541 catcttttct attgtttcct ttgccttttt agctcttgat tatacaatta atgggcacat
     3601 aggaatcatt caactagcga ttacctttgt tcctctcttt ttattatggt tagtctattt
     3661 tgtaaaatgg aattacaggc atcttaagag caaagattct ctcggagctt taggtcttgc
     3721 ttctgtttct tgggtgcttt atgctctagc ttttccgcca ctccctctcg gtgtaggcgc
     3781 tcttattttc ttagtgcctt ggtttattgt attacaaaag aaaaaacctt ctgtagccct
     3841 ttttgccact ttctggtctg gggtaatata caatgcgatt aattattact ggatttacaa
     3901 tgtaatgaaa gtggggcctg cgggctttat tttactagga ctttttctac tcattgccta
     3961 ctttagtgcc tataatgtat tagccgcttt tgtttttatt caagcacaaa aagtaaaaat
     4021 caaagggcat tcgatcttag tatggctttt tccatttttc tatgcaggct tagaaatgac
     4081 tcgaacaaaa ggagatttta gttttccttg gagccattta gggtacgctt taggaaatca
     4141 tttgtctctc atccaagctc tttcttggat aggcattttt ggatacaccg ctttaattat
     4201 aacttctaat attttagtca caaaagcttt tacagaaaag aaataccgct attttatttt
     4261 tactccagtt gtaattatcc tttgtctatg gattcaagga acaattgttc tatcggcaaa
     4321 aaccgcccag cctttttatg aagacccttc agaaaagtct ccgatgattg cgatggtgca
     4381 acctagcatt cctcaaacaa aaaaatggag taaagcctac tttgattcta taactaccaa
     4441 aacatggtct atcgtagatg agtttcctac tctgcatgaa gttgatttgc ttgttcttgc
     4501 agaaacagca atccctgata ttctaaaacg ccacccagat caaattcgtc atattcgaaa
     4561 agtagcaagt gaaaatggtt taaatctaat tgtgggtgct ttagattatg ataaaataaa
     4621 aagagagggt aaagcccctt tgtttaaatt gtataactct gcttttttat tttaccctga
     4681 ttcacacaac aaaaaaaatg aacgctatat taaacaacat ctagtgcctt ttagtgagcg
     4741 aattcctttt gatgatgtat ttccaatatt aaactatgtt gattttggcg agggcgattt
     4801 tacaccagga acagaaaccc ctgtttataa accctttgat tggactcctt atatttgtta
     4861 cgaagctatt tttggagatc aaatacgtag agccatacga aatggttctc gcataatggt
     4921 caacataact aacgacgggt ggtttggtaa aagtactgct ccaggacaac acctcaatct
     4981 gattcgatac agagcaattg aaaacggcta tcctgttgct cgtaacgcca acagcggcat
     5041 ttctgtattt attgatcaat acggtcatct agatcaacag acgggactat ttgaagaaaa
     5101 aatcattgca agaaaaatgc ctctacgcac aagagacact ctatatactc atattggaga
     5161 tgtagtagag atagggctgc ttgttttctt tgctatatac ttactctata ttttaattcc
     5221 actaatttta gataaattaa ggtatcgcaa gaataaaaaa agtgaacgat aaagcgtcgt
     5281 tcactggatt aaaagcaaag taggtcaaat actatagaag aagtgtatga ggccagtgcc
     5341 ctgatttagg gcgttcaaga agttttgcac taggaagaat ggtagatatt ttcttagcta
     5401 gcactggcaa aatcaactgg tcttctcgcc caaaataaac aatcgtttct cccttattct
     5461 caagaggttc ttcgatttta ttatggcaca aaaaatctag acctgaagcc agcgtttctg
     5521 gattcatttt caaagcagtt tccatccacg cttcttgaat caattcatca caagggccca
     5581 tcagttctag aaagctttct agagtaggct taatcgcttt tgtgacttgg cgagcaagca
     5641 tctctacatt gcgtacagac catccaccat cttcatcaca aaaatcataa acaggagcga
     5701 gcaaaatcca tttttgattt gcaggtcgat ccttcgctga aagcattaaa cacaaagacc
     5761 ctaaacccca agccacaaca caatccgcat tttttagacc agcaatagag gtgtaaagat
     5821 tttttggctg tttcattaat tgttcaaaag caataaaagt atgatcggac tcagacgatg
     5881 cttcgacaag atcgtcttcc caaatttcca tatttgcggc ccaatcagaa agccaaaacc
     5941 atttttcgct catgattgtc gtctccattc gttcaaaatt ttttctccaa taattttttc
     6001 caaaacaact atgcaatatt aaaaagctgt agaaaaaaat ggaatagccc tatatcattt
     6061 tatacaatat tttgagcgtt ttcacaagaa aaaagtttat tcttctaaat taaggatttc
     6121 tgcttgtatc gtaagttctt cgggataata ttgtaaggct tttttaagaa actgaagcgc
     6181 ttcttcatta tttttacgaa gagaggcttc ataagccaaa tttttaaagc cgtaaatggc
     6241 ttctttatta ttgtattttg ctgcttgttc gtacgcttgt tctgctcgat cccaccattt
     6301 tgcatcaaaa gcaagatttc caaggaatat ggccgcatcc gaaaagctag gcttgattaa
     6361 aagagcttgt gataaaatat ccattgccat gtattcttgc ttcccttctg caagaacatc
     6421 ggctaattta taaagcaaag aaacatcatc tggacgaatc acgagagcct ctctataggc
     6481 accagcgctt gcatcatatt cttttttaag cctgtaaaca tctcctaaat acactaaaaa
     6541 gtctatttct tctggatttt tagtgtaagc cttacgcact aagtctatcg cgagatcaaa
     6601 gtcctcaaga gcgatataag cttctgaaag ctggtataca atggtaatat cgatagaatc
     6661 taattggtag gctttttgaa gtgctttgac agtgcccact ttatcacctg ttcgtgtata
     6721 agcctcacct aagaataacc aacctgaact atttgtaggc tcaagagtta acgcccgatg
     6781 atacgtggcg atactttctg gatataattt caatcgaaaa taaacagagg ccaaattgaa
     6841 taaagctgga gaaaatttac ccatagaaaa atctaccgct tttcgataag ccgctgctgc
     6901 ttcagggtat ttctctgtct ggaataagca atttccagtg ttaaaactta ctgccacagg
     6961 atcagccccg cgagcaagag cttttcgata aagtaaaata gcctgcgggt aacgcccttc
     7021 ttgatacaaa gcattcgccc tatctaaaag agcatcactt tcttgaacgg caaatgcaca
     7081 gacggttaag aaacttataa agaaaaaaat ggctttcata caagtcgctt ttgaaataag
     7141 attttttttg tttatcattg ctttcatcct attttctttc tctnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnncctttgc ccacaggcat ttcttctaaa actcgaatat
     7321 cataaacacg cccttgaaca tctacacaaa aagaaagccg tagtaatgcg tcttgttcat
     7381 tatctctaat gctttgcgga ggagaaaacg cgggcaaacc acgacttgtc ggtttttcat
     7441 cgacgtctcc agtttctttt gtaaagcccc cgcctcgaat tcctgtgacg agttcatcat
     7501 ttatagcagc tccatcagca ccacccacgc tatttaaatc cattgaaaaa cgagggcctg
     7561 atttgggcga acgacttgtg gatttttgac ggctgggttt tcgagtgggc tttttctttt
     7621 caatttgttt tttgacttct tctggtttct taatctgtat ttccgtttta acgtatttat
     7681 tggtgcgcgt aaaatcgcct ttaataaaat agtttgcaag agtcaccgag aaaacgagca
     7741 agaaactaga cagtaccgct gcaacaaaaa tcaaaaatcg cttaatccat ttcatagaaa
     7801 acccactaat cggattgcgc cgctatcgaa acttttttaa cacctgataa gttacaggca
     7861 tcaatcacct gaactaaagt tcctgtaact gcccctttat cagcaatgac aacggcttct
     7921 cgatctggag tttttgcaag tgtttgtttc aagatatctt gtaaagaagc catatccact
     7981 tggcgttcgt gaatatgaat cgttccttca cgagtaatag caatcaaaat attttctttt
     8041 tctaattgac ttgcactttg tgcttggggc tttgttacat ccacacctgt ttctctagtg
     8101 aatgacgagg tcaccacaaa aaagattaaa agaataaaaa cgatatccat taaaggaccc
     8161 atttctattc ccatctcttt tcttttacga cgtggtatat taaaatccat ctaagcctcc
     8221 tgtcgttttt ctataaagtt gaaaataatg gtggcaccta attctatttg atctcgaatc
     8281 atatcaatcc gttcatctaa tcgctgtttt aagagcgtta aagggaaagc aactaacaag
     8341 ccactttgag tagtaatcaa agcttctgaa ataccatcgg ccattaatac tgggttttga
     8401 tttccataaa gagtaatcac atcaaaagtc gctaccatcc ctgtcacagt acctaaaagc
     8461 cctaataatg gagctgcagc ggccatcata gaaatcacat gagttccttg ttctatatag
     8521 cgagaagttt gcaataatcg aatggaaata taatcctcaa atcctgctga attttctttt
     8581 ccttttttta aggctaaaaa aagttcacga gctacaatac ccatattact ttttaagaat
     8641 tgttcggcct gatccacatc ccctttttct aataaaactc tcatgcgttc tacattttta
     8701 ctaagatcat ttttaaa
//
LOCUS       DAOOBB010000072         8638 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_10044_length_8638_cov_5.324013, whole genome shotgun sequence.
ACCESSION   DAOOBB010000072 DAOOBB010000000
VERSION     DAOOBB010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 8638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8638
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10044_length_8638_cov_5.324013"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="PLT31_04685"
     CDS             complement(<1..526)
                     /locus_tag="PLT31_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94469.1"
                     /translation="MSRTINVVLLGLSFLLISPLLVKAEEFQRWSALPVLGYSEETKW
                     QYGAIALLFFKPESKGIPGSSLDFSVIGTTRKQLQVMTSPDLYLWHGQIHIDLELVYW
                     NWIAHYFGIGNNPDVDEYLTYDMTRYRLRIPAETKLFLPSSLSFMKYGALAHIEYRDI
                     DFRQKESDLSEPSTT"
     gene            complement(511..1485)
                     /gene="hemH"
                     /locus_tag="PLT31_04690"
     CDS             complement(511..1485)
                     /gene="hemH"
                     /locus_tag="PLT31_04690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HPW94470.1"
                     /translation="MDMEMKEGVLLLQLGSPKAYTTEAVKEYLLSFLGDPHTLGSPPF
                     FWNPLLRYIIAPSRAKRSALKYKEMCEIQGVTEMPLITHTRNFVQGVQAKLKDRAFVR
                     YAFQYGASPSIQEALDDFVKEGVKKVRVIPLYPQRSGATSVSALDQLKENRAKYPSLD
                     FYACHGFADHPAWIENVVQTIKTYDKDSSYTILISWHGMPQKRIRLGDPYESDCQKSV
                     LAIEQKLGRSVLVSYQSRFGVGKWLGPSTNDTLTELGKAGAKVIVVCPAFTVDNLETF
                     HEIDIEAKKIFLNAGGQDWQRVPCLNASPKWITDFANHIALDLPCQEL"
     gene            1578..3029
                     /locus_tag="PLT31_04695"
     CDS             1578..3029
                     /locus_tag="PLT31_04695"
                     /inference="COORDINATES: protein motif:HMM:NF037658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94471.1"
                     /translation="MDIKTRISELLRILSNDLFDRESIFELTFLALFTEQPVYIFGRS
                     GSGKNIIAKRAIQMFRDINVLTFAKRYQEIPKNLEDYQIVFFQGFDSKNPLCNNFVNI
                     TIQEKSARSLIMTSKERPEASLAEAGIADQVLFILSLPEIYSVNSLQKLLKENFNINN
                     IIAPVDLLITKEEQIEWLKKIQTVELSDDTLKMIGQIAECCNENQVYVSIQKWLAFSL
                     IIKTIAFFNGRSQTNITDTFFLGTPIWIRKAQNETITTCFKNSLTQWLYNRFPLISNL
                     EESLTQLKQEVEHAINSNGDVYETILFNNIECIQYSITVAGESIPLYTPAAYIGTNQE
                     FNPFNELKQKEKRVICNFMGDNVCRISIDSSAKKTGLRATTNASFGKTFEPFAKLPAR
                     VLRINDEEKIKKNKAIIEELKQTLVSTIEKNASAMIEFKELYKEVKNNKNNLFLNTEY
                     FNYLQEAIHTQFSKISEHIQILKSLQESLMQKK"
     gene            complement(3034..3738)
                     /locus_tag="PLT31_04700"
     CDS             complement(3034..3738)
                     /locus_tag="PLT31_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94472.1"
                     /translation="MTFGFIGLHIVRLEIGLNIFSIFYTVFDLLYPFFFLILFAGNLC
                     LLKALLVRIPFDKHSLNTLQFLVKSNFIVFGFLTFATMLFSTEPTSELAYTFWLACFI
                     VGLLLNISIGIYLMIQARTERKFALLWIGFAFVLAQFFDYSGFRVLLVLTENVTPDFI
                     WNYIGLSFFDYASMDSEIKNMFDEITSMSLFLTLSLGALVVILRTVALAVFYVLMAQL
                     LFIRKKPKKMMLKSDR"
     gene            complement(3801..4178)
                     /locus_tag="PLT31_04705"
     CDS             complement(3801..4178)
                     /locus_tag="PLT31_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES family protein"
                     /protein_id="HPW94473.1"
                     /translation="MMESIKNLVMIGDRVLIKPLATTNKTGSGLYLPPSVKEKDAVHT
                     GVVIKVGPGFPLPTNQDADSFLHEKSESVNYLPLQVKEGDQALYLHSNQLELEFNNEK
                     FVVVNQNAILLVFRDDLADLGIE"
     gene            4797..5315
                     /locus_tag="PLT31_04710"
     CDS             4797..5315
                     /locus_tag="PLT31_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94474.1"
                     /translation="MPVLFNADSSSLSLKAIGFGVFNNRGFRIKKSLQKKLGNDYIVS
                     FIKPNEILIQYKGYPWNMELKLSLPLLKIAQQEQLDPALLLSLIKNQEGFIPLQKAPD
                     FNRFRERANLLKERLSNFSSMEDALASIYFNVDSPKDLPLKWWKQPLARNWVHQVFID
                     AQIYREYGFSLE"
     gene            5448..5585
                     /locus_tag="PLT31_04715"
     CDS             5448..5585
                     /locus_tag="PLT31_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94475.1"
                     /translation="MINNQIRHERRNRQYEPEYTWDRFFTNAKTQKIKGSNEPWWYDE
                     K"
     gene            5672..7519
                     /locus_tag="PLT31_04720"
     CDS             5672..7519
                     /locus_tag="PLT31_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW94476.1"
                     /translation="MIQIQNISKDFGSQTLFKEAGFLVAPLERVGVIGRNGSGKSTLF
                     KIILGEEDLDGGSICIPKNYSFGYLTQHIHFSHPTVREEACSVLVENEEGWLETHKVE
                     AILFGLGFDEESMDKSPSILSGGFQIRLNLAKVLASEPKMLLLDEPTNYLDVVSTRWL
                     SRFLRQWKGELLLITHDRHFMDSVCTHTVGIHRQKMRKIQGSVQKLKDTIAEEEEVFI
                     RTQENEAKKREQLEKVIDKFRFKASKAAMVQSKLKTVAKLGPIERLSHERNLDFSFTE
                     APFPGKRMLQIKNLSFAYPNGPQLIEDLSIEIFKGDRIGIIGPNGRGKTTLLNLIAHE
                     LSPSNGQIDYNPNLKVNYFGQTNINRLNLENTVEEEIQTAVPDSSRGRARSLAGLMMF
                     SGDTALKKVRVLSGGERSRVLLGKILATSCNLLLLDEPTNHLDMESIESLIDALEDYE
                     GTAMVVTHDEELLHAFANRLIVFDQGECFSFEGSYQDFLETIGWKSEKKEAMSSLPLA
                     QAEKKETVVSNKENRKARADYIAERSRLLKPLEKELSRVEESIKKQEDLALELEQKLV
                     MASEQNNGELIASLAKELDENKKQIEHLYDSWEMVTANLQEVSEKYPLD"
     gene            complement(7524..>8638)
                     /locus_tag="PLT31_04725"
     CDS             complement(7524..>8638)
                     /locus_tag="PLT31_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94477.1"
                     /translation="KESGISSLIKDDIPDSLSWFYGDSTWRYIGGEDLLGLIQDSSVI
                     ITADSLIDIVFTGLEGTIYKGSSKTQFSYIPSEGVDSGKVQVMKVTGVISDKIGPIIT
                     NAFVTPKSENVSLLTLTFSESLDTTSISMDSILEFKVWREGVEVSGALEISSQTRLQK
                     GKRYDLYFVLNEKSVLLTVGDSVRFAPGIARDLNQNYPHINNPYVRIIGEQFTTVDVT
                     ELIIIDSDTRDTDTLPPMIVKAFPLTETFKNAEKEMGLPGHLIRYDLNELLMTYPDVK
                     KEDVFIEYEAFYFTNLGVYVNSHKDKIRCTDKVFNGDCSKNPGNIYLAWNTRSEKGRK
                     VGTGAYISRLTFKIQAGTHLVDKKNETNSFGIKRNK"
BASE COUNT         2947 a         1629 c         1404 g         2658 t
ORIGIN      
        1 ctgttgtaga tggttcgctc aaatcacttt ctttttgtct aaaatcaata tctctatatt
       61 cgatatgagc taaagcgcca tacttcataa aagacaatga agaaggtaaa aagagctttg
      121 tttcagctgg gattctaagt ctataacgag tcatgtcata ggttaaatat tcatcgacat
      181 caggattatt tcctattcca aagtaatgag cgatccaatt ccaatagact agttccaaat
      241 cgatatgtat ttgcccatgc cataaataaa ggtctggaga tgtcattact tgtagctgtt
      301 ttctagttgt tccaattaca ctgaaatcta atgaagagcc aggaatgcct ttggactcgg
      361 gcttaaaaaa aagtaaagca attgcaccgt actgccattt agtttcttca gaatacccca
      421 aaacaggtaa agcactccaa cgttgaaact cttctgcttt cacaagcaat ggagagatca
      481 ataaaaaaga taagcctaaa aggacaacgt ttatagttct tgacacggta aatccaatgc
      541 aatatgattt gcaaaatcag tgatccattt tggagaagca ttcaggcacg ggactctttg
      601 ccaatcttgc cctccagcat ttaaaaagat ttttttagcc tcaatatcga tttcatgaaa
      661 cgtttctaaa ttatccacag taaaagcagg acaaacgact atgacttttg cccccgcttt
      721 ccctaattcc gttaatgtat cgtttgtaga agggccgagc catttcccta cgccaaaacg
      781 actttgataa gaaactaaaa cggaacgccc taatttttgt tctatcgcta atacactctt
      841 ttgacaatca ctttcatacg ggtctccaag acggattctt ttttgaggca tgccgtgcca
      901 agagattaaa atagtgtaag aggaatcttt atcgtacgtt tttatcgttt gaacaacatt
      961 ttctatccac gcaggatgat cagcaaaacc atggcaagca taaaaatcta acgaaggata
     1021 ctttgctcga ttttctttca attgatctaa agccgagacc gaagtagctc ctgaacgttg
     1081 tgggtaaaga ggaatcaccc ttaccttctt aacaccttct tttacaaaat catctaaagc
     1141 ctcttgaatg gagggacttg ctccatactg aaaggcataa cgcacaaaag ctctatcttt
     1201 taatttagcc tgcacccctt gcacaaaatt tcttgtatga gtaattaaag gcatttctgt
     1261 tacaccctgt atttcacaca tctctttata ttttagagcc gatcgtttag ctcgtgaagg
     1321 ggctatgata taacgaagca aaggattcca aaaaaaaggt ggagaaccaa gcgtgtgagg
     1381 atctcccaaa aaagaaagta aatactcttt taccgcttca gttgtatatg cttttggaga
     1441 gcctaattgc agaagtaaaa cgccttcttt catttccatg tccattatat tatcttattg
     1501 tagaaaaaat aactatttca aaacaataaa attttatatt atagagattc taatagaatt
     1561 cttgatagag gtgaatcatg gatattaaaa ccagaatttc ggagctactc cgtatattat
     1621 caaatgatct atttgatcga gaatccattt ttgaactcac ctttttagcg ctttttacag
     1681 aacagcctgt ttatattttt ggtcgttctg gttctggaaa aaatattatt gcaaaacgag
     1741 ccattcaaat gttccgagac atcaatgtct taacttttgc caaaagatat caagaaattc
     1801 ctaaaaattt agaagactat caaattgtat ttttccaagg ttttgacagt aaaaatcctc
     1861 tatgcaataa tttcgttaat attaccatcc aagaaaaatc agctcgttct ttgattatga
     1921 caagtaagga acgccctgaa gcttctcttg cagaagcagg cattgcagat caagttttat
     1981 ttattctttc gttacctgaa atatattctg tcaattctct tcaaaagcta ctgaaagaaa
     2041 atttcaacat caataatatt atagctcccg tcgaccttct gattactaaa gaagaacaaa
     2101 tagaatggtt gaaaaaaatt cagacagtgg agctttctga cgacacgttg aaaatgattg
     2161 gtcaaattgc agaatgctgc aatgaaaatc aggtttatgt ttctattcaa aaatggcttg
     2221 ccttctcttt aatcattaaa accatagcct tttttaacgg acgaagccaa acaaatataa
     2281 cggatacttt ctttttaggc acccccattt ggataagaaa agcccaaaat gaaacgatta
     2341 ccacttgttt taaaaatagt cttacccaat ggctatataa tagatttccc ttgatatcca
     2401 acttggagga gtctttaact caattaaagc aagaagtgga acatgcgatc aattcaaatg
     2461 gagatgtata tgaaaccatt ttgttcaata atattgaatg tattcaatat tcaattacgg
     2521 tagctgggga atccattcca ctatatacac cagcagctta catcggcacg aatcaagagt
     2581 ttaatccatt caacgaactg aagcaaaaag aaaagcgagt gatttgtaat tttatgggag
     2641 ataacgtttg ccgtatctcc attgattctt ccgctaaaaa aacaggtcta agagcgacca
     2701 caaatgcttc ttttggaaaa acttttgaac ctttcgctaa attacctgca agagttcttc
     2761 gaatcaatga tgaagaaaag attaaaaaga ataaagccat aattgaagag ctaaagcaaa
     2821 cacttgtttc tacaatagaa aaaaatgcga gcgccatgat tgaatttaag gaactctata
     2881 aagaagtgaa aaacaataaa aataatttat tcttaaatac tgagtatttt aattatcttc
     2941 aagaagctat acatactcaa ttctctaaaa ttagcgaaca tattcagatc ttgaaatcat
     3001 tacaagaatc attaatgcaa aagaaataga caattaacga tctgatttta acatcatctt
     3061 ttttggcttt tttcgaataa aaagaagctg cgccattaat acataaaaga cagctaaagc
     3121 gacggttcga aggattacca ctaacgctcc taatgataag gtcaagaaaa gactcattga
     3181 ggtaatttca tcaaacatat tctttatttc tgaatccatg gatgcataat caaaaaaaga
     3241 aagtcctata taattccaga taaaatctgg ggtaacattt tctgtcaata caagaaggac
     3301 tcgaaagcca ctatagtcaa aaaattgagc taaaacaaag gcaaaaccga tccataaaag
     3361 agcaaattta cgttcagtcc tagcttgaat cattaaataa ataccaatac taatatttaa
     3421 taaaagccca acaataaaac aggcaagcca aaaagtataa gcgagttcag aggttggttc
     3481 cgttgaaaaa agcatagtcg caaaagtcaa aaagccaaaa acgataaaat tggattttac
     3541 taaaaattga agtgtattta gcgagtgctt atcaaaaggg attctcacta atagagcttt
     3601 taagagacaa aggttgccag cgaataagat caaaaagaag aaaggataca acagatcaaa
     3661 aacggtataa aaaatggaaa agatgtttaa tcctatctct aaacgtacaa tatgaagacc
     3721 tataaaacca aatgtcaaaa atggcattat caatgtggaa attgcaccta acaacaaagg
     3781 catcttataa cttgtactag tcattctata cctaaatcag caagatcatc tcgaaaaact
     3841 agtaaaatgg cgttttgatt taccaccaca aatttttcat tattaaattc caattctaat
     3901 tgattactat gtaaataaag agcttgatct ccttctttta cttgtaaagg caaataattc
     3961 acagactctg atttttcgtg taaaaaagaa tccgcatctt gatttgtggg caaaggaaaa
     4021 ccaggcccta cttttatcac aacaccagta tgtacagcat ctttttcttt gacacttgga
     4081 ggtaaataaa gaccgcttcc agttttatta gttgtcgcta atggttttat taaaacccga
     4141 tcgccaatca tcactaaatt tttaatactt tccatcatct ttaaaaatta gtatataatt
     4201 cttgaatata cccttttgtt tttcttcttt tactgatggt ctcgtctaaa aaatcatttt
     4261 catgattaca tctcgctacg ttgtctctta aagacaccca ataccaccag aacgctccac
     4321 ttttcgttcc tagtatcgtt tggaccttga acaaatcatt taaccaaagc tatttttaga
     4381 ggtaagctta tattagtagt atgttctttt tacgtgttgc aattatcctt gccttaatca
     4441 taggcctcct gctttttctt tccgcaaaag ggagagcata tttccgcctt tttaaaaact
     4501 tttttcatga taggcctgtt tttataatta tccctatttt aggcttaatt ctactaatca
     4561 ctcttttctt ttttcctcct ctctcatcct caagacctgc tctgaatttt gaggatcaaa
     4621 aaaaacacca agactcccta attcttcatt ttacagaaga aacaattctt gatctggcta
     4681 aaaaaattca aatgcctatt gattcccaat tgaatttaaa gcttatcagg cctgagctta
     4741 tttatatact cccgacatgc ttaaacgtat ttaaaagcat tactaaaaaa gagacgatgc
     4801 ccgtcttatt taatgccgat agctcttctc tatccttaaa ggcaataggc tttggtgttt
     4861 ttaataatag aggttttaga attaaaaaga gtcttcaaaa aaaattagga aatgactata
     4921 tcgtttcttt tatcaaacca aatgagattc taattcaata caaaggctat ccttggaaca
     4981 tggaactaaa gctatctctt cctcttctaa agattgcaca acaagaacaa ttggatccag
     5041 ctcttttatt gtccttaatc aaaaatcaag aaggttttat tccccttcag aaagcccctg
     5101 attttaatcg gtttagagaa agagctaact tacttaaaga gaggttaagt aacttctcat
     5161 ctatggaaga cgcccttgcc tcaatctatt tcaacgtgga ttcacctaaa gacttgccat
     5221 taaaatggtg gaagcaacct ctcgctcgaa attgggttca tcaagtattt attgatgctc
     5281 aaatctatcg tgaatatgga ttttctctag aataaccaca tttatccgat ttttcttata
     5341 ttttttttct tttcatttgt atctatagta ttttttatat atttaccact aagttatcat
     5401 aaaaagaaaa gcaatcgctg ggaaaaaaac ctcagcaagg agataaaatg ataaataacc
     5461 aaattcgcca tgagcgaaga aacaggcagt atgagcctga atatacttgg gatcgtttct
     5521 ttactaatgc aaaaacgcaa aaaataaaag gaagcaacga accttggtgg tacgacgaaa
     5581 agtaattaga gttagtatct gacaaagcct cggcaaaatt tgctggggct tttgaatttt
     5641 aaaatcttga atataaaact atatttgcgt cgtgattcaa attcaaaaca tatcaaaaga
     5701 tttcggctct cagactctat ttaaagaagc tggtttttta gtcgctcctc tcgaacgcgt
     5761 tggcgttatt gggcgcaatg gttcagggaa gtccacacta ttcaaaatta ttctcggcga
     5821 agaagactta gatggaggga gcatttgtat tcccaaaaat tatagttttg gatacctcac
     5881 tcaacacatt catttttctc accccaccgt tcgagaagaa gcctgctctg ttcttgtaga
     5941 gaatgaagaa ggatggctag agacacataa agtggaggct attcttttcg ggcttggctt
     6001 tgatgaagaa agcatggaca aaagtccttc tattctttct ggtggatttc aaattcgatt
     6061 gaatctcgct aaggttcttg cctctgaacc taagatgctt ttactagatg aacctacaaa
     6121 ctatctggat gtggtttcta cgcgctggct ttcaagattt cttagacagt ggaaaggcga
     6181 acttctttta ataactcatg atcgccattt tatggattct gtttgtactc atactgtagg
     6241 gatccatcgc cagaagatgc gtaaaattca aggctctgta caaaaattaa aagataccat
     6301 tgctgaagaa gaagaagtat ttatacgcac ccaagaaaac gaagctaaaa aaagagaaca
     6361 acttgaaaaa gtgattgata aatttcgctt taaggcaagt aaagccgcca tggtgcaatc
     6421 gaaacttaaa acagtggcga aactagggcc aatagaacgc ttatctcacg aaagaaactt
     6481 agacttttcc tttactgaag ctccatttcc tggcaaaaga atgcttcaaa ttaaaaatct
     6541 ttcctttgct tatccaaatg gtcctcaatt aatagaagat ctttctatcg aaattttcaa
     6601 aggggatcgt attgggatta ttggtcctaa tggaagagga aagaccactc tacttaacct
     6661 tattgcacat gagctttctc caagcaacgg acaaattgat tataatccaa acttaaaagt
     6721 caactatttt ggacaaacaa atatcaatcg cttgaatttg gaaaacactg ttgaagaaga
     6781 aattcaaaca gctgttccag attcttctcg tggtagagct cgtagtcttg cagggctaat
     6841 gatgttttca ggagatacgg cgttaaagaa agtacgcgtg ctttcaggtg gcgaacgcag
     6901 ccgagttctt ttagggaaaa ttcttgcgac ctcatgcaat cttttgcttc ttgacgaacc
     6961 cacaaaccat ttagacatgg aatctattga aagcttaatc gatgctcttg aagactatga
     7021 aggcacagcc atggtcgtca cgcatgatga agaactactt catgcttttg ccaatagact
     7081 cattgttttt gatcaaggcg aatgcttttc ttttgaaggc tcttatcaag actttttaga
     7141 aactattggc tggaaatcag aaaagaaaga agccatgagt tctttacctc tggctcaggc
     7201 agaaaaaaaa gaaacggttg tttcaaataa agaaaataga aaagcacgtg cagattatat
     7261 tgctgaacgt tctcgtttat taaagcctct agaaaaagaa ctttctcgtg tagaagagtc
     7321 tataaaaaaa caagaagatt tagctctaga actagaacaa aagcttgtaa tggcttcaga
     7381 acaaaataat ggagagctta tcgcctcgct tgcaaaagag ctagacgaaa acaaaaagca
     7441 aattgaacat ctctacgatt cttgggaaat ggtaacggca aatctccaag aggtatcaga
     7501 aaaatacccc ttggattaat taatcattta ttccgtttga ttccaaaaga attagtctca
     7561 tttttcttat ctaccaaatg agttcccgct tgaattttga atgtcaatcg agaaatatac
     7621 gcacctgttc ctacttttcg tcctttttca cttcttgtgt tccaagctaa gtatatgtta
     7681 cctggattct tagaacaatc gccattaaat actttatccg tacaacgaat cttgtcttta
     7741 tgagagttca catatacacc taaattcgtg aaataaaacg cttcatattc gataaagaca
     7801 tcttcttttt tcacatcagg gtaggtcatt aataattcat tcaaatcata tcgaattaaa
     7861 tgacccggaa gccccatttc tttttctgca ttcttaaatg tttccgttaa tgggaacgct
     7921 tttacaatca ttggaggcaa tgtatccgta tctctcgtat ccgaatcaat aataattaac
     7981 tcagtaacat caacagtagt aaattgttct ccaataatac gtacataagg attattaata
     8041 tgcgggtaat tctgattcaa atctctcgca attcctgggg caaatcgcac actatcacca
     8101 acagtgagca gcacagattt ctcatttaag acaaaataga gatcataacg tttccctttt
     8161 tgaagccttg tttgactact tatttctaaa gcaccagaga cttcaacgcc ttctcgccaa
     8221 accttaaact ctaaaatact gtccattgaa atacttgttg tatctaatga ttcactaaaa
     8281 gttaacgtaa gtagacttac attttcagat tttggagtaa caaaagcatt cgtaattata
     8341 gggcctattt tatcagatat cacccccgtg accttcatta cttgaacttt cccagaatcc
     8401 acgccctctg aaggaatata agagaattga gttttagaac ttcccttata aatagttcct
     8461 tctagaccag taaatacgat atcgattaaa gaatctgctg taataatcac agaagaatct
     8521 tgaatcaaac caagtaaatc ttctcctcct atataacgcc atgtagaatc gccatagaac
     8581 caagataaag aatctggtat atcgtctttg atcaaagacg atataccaga ttctttat
//
LOCUS       DAOOBB010000073         8581 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_10115_length_8581_cov_5.345883, whole genome shotgun sequence.
ACCESSION   DAOOBB010000073 DAOOBB010000000
VERSION     DAOOBB010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 8581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8581
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10115_length_8581_cov_5.345883"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..882)
                     /gene="panB"
                     /locus_tag="PLT31_04730"
     CDS             complement(139..882)
                     /gene="panB"
                     /locus_tag="PLT31_04730"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HPW94478.1"
                     /translation="MLSIKDIQNKKKNGEKVSVITAYDFAFAKMAEAAHIDQILVGDS
                     LANVMLGYSSTREIGMNEMIIFTRAVSKGAPNTHIISDMPYLSDTNPTLALENAKLLL
                     DAGAHSVKLEGAKEDVIEILLKNNIPVMGHLGLLPQTAKSFKQFGKTSEEADQLLKDA
                     MTIDKLGVYSMVLEHVPETLGTLISHRVSAVTIGIGAGNSTDGQVLVLHDVLGLNGGK
                     IPPFAKKFANIFEIGVQGISDYIQSIAKK"
     gene            complement(870..1604)
                     /gene="recO"
                     /locus_tag="PLT31_04735"
     CDS             complement(870..1604)
                     /gene="recO"
                     /locus_tag="PLT31_04735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HPW94479.1"
                     /translation="MLIKSPSIILHRYPYSDSSWVVKALTASQGVVSFLVKGGKRHEF
                     SFKNALDPLSFSEIVFKKTSKSELYFIKEASLIKWFPEMRNNLEALAIAQVMAEVILR
                     YTPQEAELEKEFSLLLNAFESLDSSPISPSILVQWLFEINDLWGYTIAIQYCVKCGTT
                     LKEAPGDFFPELGGCLCKNCASDSVPRANPLFLKDLWLFNQNMPLEKTPLMEKAFLLY
                     LQRHLGSSQEIHSVQWLKEVRKICSQ"
     gene            complement(1609..1953)
                     /locus_tag="PLT31_04740"
     CDS             complement(1609..1953)
                     /locus_tag="PLT31_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HPW94480.1"
                     /translation="MSDCLFCKIISGEIPSKKIYEDDDVFAFHDIAPKAPTHFLVIPK
                     KHIPHVMDLEKEDSILMGKILFQAQRIAKVLGLEPNGARFVFNCKESAGQTVWHIHLH
                     VLGGRDFDWPPG"
     gene            2222..4594
                     /locus_tag="PLT31_04745"
     CDS             2222..4594
                     /locus_tag="PLT31_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HPW94481.1"
                     /translation="MNFSAVISEELKLEEWRVAKALELMEQGGTVPFIARYRKDQTGT
                     LNEIELRDIAHRREYLQEIKERRETILKSIEEQGKLTPELKAQIENCKEKTLLEDLYA
                     PYKPKKRTRATIAKELGLEPLARTIMAQEPVSNTAEDIGRIYLSEEKGLADPKAAING
                     ACDILAEEVADNAEYRQYLRTQMEKQGVMISKVRKEFEKQETKFSNYYDFSEPLAKIP
                     SHRMLALRRGEKEKVLRLTIEVPDDILVSYLQNQIIKGDSIWKSYLQDMCKDAWERLL
                     KPSMESEVRMLLKDAAEEEAFKVFSKNLQDVLLAPPAGHKAVLALDPGFRTGCKVAVL
                     DENGKFMDHGVIYPHEPQKQTVEAAAYIMQLIEKYKIDLIAIGNGTASRETDAFCTAI
                     AAKIKGKMPPRVIVSESGASVYSASMIAIEEFPKEDVTTRGAICIGRRLQDPLAELVK
                     IDPKAIGVGQYQHDVNQRELKKRLDEVVESCVNLVGVDVNSASAPLLGYVAGVSKNLA
                     ESIVKHRDQNGAFKSRKDLLTVKGFGPKAFEQAAGFMRVSGSENPLDCSAVHPENYPL
                     VESIAEKVGVPVVELVGNASAVRGINVEEFLSENVGKHTLEDIIAELEKPSRDPRKEF
                     RYAKFDDKIQTIQDLITGSWMEGVVTNVANFGAFVDIGVHQDGLVHVSEISDKFVDDA
                     KTVLTVGDVVKVRVMAVNVEQKRISLSMKQESLDEGNVAGAGQNAKSRFPQQKNRRNE
                     RPAVVQGHATIADLKAKLAGKNVAKQQPAKKPQVQISKVNSMLKQIMKKG"
     gene            4712..4975
                     /locus_tag="PLT31_04750"
     CDS             4712..4975
                     /locus_tag="PLT31_04750"
                     /inference="COORDINATES: protein motif:HMM:NF024086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPW94482.1"
                     /translation="MKKNKFCQSCGMPMNKDPEKGGTEKDGSKSELYCSYCYQSGAFC
                     SPDIDTAEKMQKFCIEKMREQGMPKFIAWLFTRVIPKLTRWKE"
     gene            5181..5567
                     /locus_tag="PLT31_04755"
     CDS             5181..5567
                     /locus_tag="PLT31_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94483.1"
                     /translation="MGGSDVQPEINPAPMENTSENGSSSNEFCTFQAVPIGNVEYTML
                     KNVKAAKGVYGSVKDILPLFETRTKDRGGNAVVNFRSGQTFGFWPWNLVRPYAKGRAI
                     NITNTNGMTCKEMGGETMSEVLARYQ"
     gene            complement(5617..6501)
                     /locus_tag="PLT31_04760"
     CDS             complement(5617..6501)
                     /locus_tag="PLT31_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPW94484.1"
                     /translation="MELSHLRYFLEVAKNEHITKSAKNLCIAQPALTHAIHKLEDELE
                     VPLFESKGRNIKLTEYGKFFYKQLEPLYLDILNLPKQLKAMASKDNSTVFLNILAASS
                     SVAKAVIVYKQKEPNLNFNLIQNEESKLFDISIKTVANYKEIPEEDVKNFIFSEKILL
                     AVPNTSKYRQRTEISLKEIQEENFIELYGSKQFRAICNSFCKEIGFSTHIAFESDSAD
                     VVKEAIAAGIGVGFWPEYSWGKIDKRRIRLLKITDGDFKRNIIITYKQNKQNNEKAEG
                     FYNFLTDYLTSSWKKRRV"
     gene            6628..6921
                     /locus_tag="PLT31_04765"
     CDS             6628..6921
                     /locus_tag="PLT31_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94485.1"
                     /translation="MSNALEAMMLICFGLSWPISVYKNIKSHSAKNMSFRFNLLIIIG
                     YLAGILAKCYMQAFNYVLVVYVVNLMIVSMNMVVYFTNKKYDEMAYQGEVYAK"
     gene            6911..7483
                     /locus_tag="PLT31_04770"
     CDS             6911..7483
                     /locus_tag="PLT31_04770"
                     /inference="COORDINATES: protein motif:HMM:NF021143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HPW94486.1"
                     /translation="MPNKNITVLFILMTLLLFVGCKDNRSCNYDPETETLECSEATYK
                     TAQVNGKTWLAENLNWPVVDSSFCYANDFGNCIKYGRLYTWGAAMNLTNKTSENPQKG
                     ICPQGWRLPTSQEFESALKSADGKKLNVIRAGFRYNNDSYVDENKSASFWTASEFDST
                     RAYLVRVLVEQSMLEHYSKTIASSIRCVMD"
     gene            complement(7604..8500)
                     /locus_tag="PLT31_04775"
     CDS             complement(7604..8500)
                     /locus_tag="PLT31_04775"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HPW94487.1"
                     /translation="MNKILFSILSLVAFSFAANIQIVAPTGADFSPDAPNMVSTIIRS
                     AVSQAGHTPTEKEESIKLTTNLMTMGSSIIVVCEQTKKGVIVGSGKQKAASIDDLDIA
                     IEQAVASALGSKTGSQEASASQPQEKEAEEDYLEKTPARKYTSVGFGFAYWHNWKDKE
                     HNPSRDFESAYALRLGYIWENSSSTALVLQSYTVFNIGDDSHVQEVLTIGGRYLFNTG
                     SIAPYLGAGFGFGIQADGHFDRFKEGFAFGFAGDVDAGVIFFQKSSLQLELGLRYNIL
                     WDGLTSFSHNFGAASAFIAINF"
BASE COUNT         2889 a         1553 c         1610 g         2529 t
ORIGIN      
        1 ggagcttttt ttggtgttgc catgattttt cttcctttta gtgaggggtt aaaaatgata
       61 ataataaaat aacttgattt tcacaaataa tcaacacctt tcttcatttt ttttattttt
      121 tttttgacaa accaaaaatt atttcttagc aatcgattgg atgtaatctg aaattccttg
      181 cacgccgatc tcaaaaatat tcgcaaactt ttttgcaaag ggtggaattt ttcctccgtt
      241 caaacctaag acatcatgca aaacaagcac ttgaccatcc gtagaattgc ctgcgccaat
      301 tccaatcgta acagcgctaa ctctatgact gataagggtt ccgagtgttt caggaacgtg
      361 ttctaagacc atagaataga ctcctaattt gtctatagtc atcgcgtctt ttaaaagttg
      421 atcagcctct tcagaagttt ttccaaactg tttaaaactc tttgctgtct gaggtaataa
      481 acctaaatga cccatcacag gaatattatt cttcaaaaga atttctatca catcttcttt
      541 tgcgccttct aattttacgg agtgagcgcc tgcatctaat aaaagttttg cattttctag
      601 tgctaacgtt ggattagtat cactaagata aggcatatcg ctaataatat gagtgttcgg
      661 agccccttta gatacagcgc gagtgaagat aatcatttca ttcatgccga tttctcgtgt
      721 gcttgaatag ccaagcatta cattagcgag agaatctcca actaaaattt gatcaatgtg
      781 agccgcttct gccatttttg caaatgcaaa atcataggcg gtgataacag agactttttc
      841 tccgttcttt tttttgtttt ggatgtcttt tattgagagc atatttttct aacctctttt
      901 aaccattgaa ctgaatgaat ttcttgagat gaacctaaat gacgttgtaa ataaagtaaa
      961 aaagctttct ccatcagagg agttttttct aatggcatat tctgattgaa caaccataaa
     1021 tctttgagga acaacggatt agctctagga acagaatcgc ttgcgcaatt tttacatagg
     1081 caaccaccaa gttctggaaa gaaatctcct ggagcttctt ttaaagtggt tccgcacttt
     1141 acacagtatt gaattgcaat cgtataaccc cacaaatcgt taatttcgaa aagccattgt
     1201 actaaaatag aaggagatat tggagaagaa tctaaagatt caaaagcatt gagtaaaaga
     1261 gaaaactctt tttcgagttc tgcttcttga ggagtatatc ttaaaatcac ttcggccata
     1321 acttgggcaa tagctaaagc ctcgagatta tttctcattt caggaaacca ttttattaaa
     1381 gaagcttctt taataaagta aagttcactt ttagaagtct ttttaaagac aatttcagaa
     1441 aaagaaagag gatctagagc atttttaaaa gaaaattcat gccttttacc ccctttcact
     1501 aaaaaagaaa caacgccttg agaagcggtt aacgctttca ccacccaaga agagtcacta
     1561 taagggtaac gatgtaatat aatagaggga cttttgatga gcaccgtttt agcctggagg
     1621 ccagtcgaaa tctcgaccgc ctaaaacatg caaatggata tgccaaaccg tttgacccgc
     1681 actttcttta cagttaaaaa caaagcgagc cccatttggc tcaagaccaa gtacttttgc
     1741 tattctttgt gcttgaaaaa gtattttacc cattaaaatg gagtcttctt tttctagatc
     1801 cattacatga gggatatgtt ttttgggaat gaccaaaaaa tgagtgggtg cttttggagc
     1861 aatatcatga aaagcaaaga cgtcatcgtc ttcatagatt tttttagaag gtatttcacc
     1921 agagatgatt ttacaaaata aacaatcaga catgtgtaaa aaataacatt ttatatatag
     1981 agtaaagtta aaaaaaagat ttctgaggtg ttgttcaggg ataaagacct ttttttactt
     2041 cgttagcttt attacattta tcactcaggt ttcttttaaa aatggcatcg aaagtcaaaa
     2101 aaagctaaat aagatctcgt tgacttaaag acctattttt agaagtcgct attactgcgg
     2161 tgaaaaccgt ttatgtttta cgttttaggg ttttataaaa gaggcccaca ggaataatag
     2221 tatgaatttt tctgcagtca tctccgaaga attgaaactc gaagaatggc gtgttgccaa
     2281 agcgttagaa ttaatggaac aaggtggaac tgttcctttt atagcacgtt atcgtaaaga
     2341 tcaaacggga actttaaacg aaattgaact tcgcgatatt gctcatcgtc gtgaatactt
     2401 acaagaaatt aaagaacgta gagaaactat tcttaaaagc attgaagaac agggcaagct
     2461 cactccagag ttaaaagctc aaatcgaaaa ttgcaaagaa aaaacattgc tcgaagactt
     2521 atacgctcca tataaaccaa agaagcgtac cagagctacc attgcaaaag aacttggact
     2581 ggaacccctt gctcgcacca ttatggcaca agaaccagtt tcaaatacag ccgaagacat
     2641 tggccgtatc tacttatcag aagaaaaagg ccttgctgat cctaaagcag cgatcaatgg
     2701 tgcttgcgat attctcgctg aagaagtggc tgataatgcg gagtaccgtc aatacctacg
     2761 tactcaaatg gaaaaacaag gtgtcatgat ttcaaaagtt cgcaaagaat ttgaaaaaca
     2821 agagaccaag ttcagtaact actatgattt ctcagaacca ttagcaaaaa taccaagcca
     2881 tcgtatgctt gctttgcgtc gtggtgaaaa ggaaaaagtc cttcgtctaa caatagaagt
     2941 cccagatgat attcttgtgt cttacctaca aaatcaaatc atcaaagggg attccatctg
     3001 gaaatcttat cttcaagaca tgtgcaaaga tgcttgggaa cgcttattaa aaccaagcat
     3061 ggaaagcgaa gtccgcatgc ttctcaaaga tgccgctgaa gaagaagcct ttaaagtatt
     3121 cagcaaaaat ttacaagatg tattacttgc accacctgct ggtcacaagg ctgttcttgc
     3181 tttagaccct ggttttagaa ctggttgtaa agtcgctgtt ttagatgaaa acggtaagtt
     3241 tatggatcat ggcgtgattt atccacatga accacagaaa caaaccgtcg aagcagctgc
     3301 ttatattatg caactcatcg aaaagtacaa aatcgattta attgcaattg gtaatggaac
     3361 agcaagccgt gaaacggatg ctttctgcac agcgattgcc gcaaaaatca agggcaaaat
     3421 gcctcctcgc gtgatcgtga gtgaatctgg tgcttctgtt tattctgcaa gcatgattgc
     3481 tattgaagaa ttccctaaag aagacgttac tactcgtgga gccatttgca ttggccgtcg
     3541 tttacaagat ccattagctg aattagtaaa gatcgatcca aaagcgattg gtgttggcca
     3601 ataccagcac gatgtaaacc aacgcgaact caaaaaacgc ctcgatgaag ttgtcgaaag
     3661 ctgcgtgaac ttagttggtg ttgatgtcaa tagcgcgagt gctccattac taggttatgt
     3721 ggctggtgtt tctaaaaacc tcgctgaaag cattgtaaaa cacagagatc aaaatggtgc
     3781 tttcaaaagc cgcaaagact tattaacagt aaaaggattt ggcccaaaag catttgaaca
     3841 agccgctggc tttatgcgtg tttctggttc agaaaatcca ttagactgta gcgccgttca
     3901 cccagaaaac tacccactcg tcgaaagcat tgctgaaaaa gtaggcgttc ctgtggtgga
     3961 actcgttgga aacgcttctg ctgttcgtgg cattaacgtc gaagaattct taagtgaaaa
     4021 cgtcggtaag catacattag aagatatcat tgccgaatta gagaagcctt cacgcgaccc
     4081 tcgtaaagaa ttccgttacg ctaagtttga tgacaaaatc caaaccattc aagaccttat
     4141 cacaggcagc tggatggaag gcgttgtcac taacgtcgct aacttcggtg cttttgtcga
     4201 tattggtgtt caccaagatg gtttagttca cgtttcagaa atcagcgaca agtttgttga
     4261 cgatgctaaa acagttttga ctgttggtga tgtcgtaaaa gttcgcgtta tggctgtgaa
     4321 cgtcgaacaa aaacgcatta gcctttcgat gaaacaagaa tcattagacg aaggtaacgt
     4381 tgcaggtgct ggtcaaaacg ctaagtcgcg tttcccacaa cagaaaaata ggcgtaacga
     4441 acgcccagct gttgttcaag gccatgcaac aattgcagac ttaaaagcaa aattagcagg
     4501 taaaaatgtt gcaaaacaac aaccagctaa aaagcctcaa gttcagatta gtaaagtcaa
     4561 ttccatgctc aaacagatca tgaaaaaagg ctaaaaacaa aacaaaaaaa ttaaaacaaa
     4621 cctctcgtaa tcatacggga ggttttttta ttataagtag ggttaaataa ttctatactt
     4681 tttatagaaa gcaaataaaa aaggagagag aatgaaaaag aataaatttt gccaaagctg
     4741 tggcatgcct atgaataaag atcctgagaa gggtggcaca gaaaaagacg gaagcaagag
     4801 tgaattatac tgctcttact gctatcaaag tggtgcattt tgtagcccag atattgatac
     4861 agcagaaaag atgcaaaagt tttgcatcga aaaaatgaga gaacaaggta tgccaaaatt
     4921 catcgcttgg ctttttactc gtgtgattcc aaaactcaca agatggaaag aataaaaaca
     4981 attaattaca gatgaagtaa tacactactt tttataagta gtgttttttt ttgtatcttt
     5041 cgcaaaacat ttgaatgcct attaaagagg ggattctctt aacttaggta atagtaatgt
     5101 ttatttataa ttaaaaagga agaataaaat gttgaaaaaa tattttcttg ctgctatggc
     5161 agccgtggct cttactgctt gtggggggta gtgatgttca accagaaatt aatccagcac
     5221 caatggaaaa tacttctgaa aatggaagct cctcaaatga attctgtaca ttccaagcag
     5281 taccaattgg aaatgtagaa tatactatgc ttaaaaatgt taaagcagct aagggagttt
     5341 atggttcagt gaaagatatt cttccgttgt ttgaaactag aacgaaagat cgtggcggaa
     5401 acgctgtagt taatttccgc tcaggtcaga cttttggttt ctggccttgg aatttagtac
     5461 gcccttatgc aaagggtaga gccattaaca tcaccaatac caatggcatg acctgtaaag
     5521 aaatgggtgg tgaaacgatg tcagaagttt tggccagata ccagtaatag aaatcatcac
     5581 tataacgaag agtgtaatag agcaaaacat gccaaattac actcttcttt ttttccatga
     5641 agacgttaaa taatcggtga gaaagttata aaacccttct gccttttcgt tattctgttt
     5701 attttgcttg taagtaataa taatatttct cttaaaatca ccatcagtaa tttttaacaa
     5761 acgaatccgc cttttatcga ttttacccca agaatattct ggccaaaaac caacaccaat
     5821 ccctgcggca attgcttctt tcacaacgtc tgcactatca ctctcaaaag caatgtgcgt
     5881 tgaaaaacca atctctttac aaaaactatt acagattgct cgaaactgtt tagaaccata
     5941 aagttcaata aaattttctt cttgaatctc ttttagtgaa atctcagttc tttgcctgta
     6001 tttagacgta ttaggtactg caagcaaaat cttttcacta aaaataaaat ttttaacatc
     6061 ttcctctgga atctctttgt aattggcaac cgtcttaatt gaaatatcaa aaagtttaga
     6121 ttcttcattt tgaatcaaat taaaatttaa gttcggttct ttttgcttat aaacaatcac
     6181 tgctttagca acactagaag aggcagcgag aatatttaaa aatacggttg aattgtcttt
     6241 acttgccatt gctttaagtt gctttggaag attgagaata tctaaatata aaggctctag
     6301 ctgcttataa aaaaacttac catattcggt gagcttaata ttacgccctt tactttcaaa
     6361 taaaggaacc tctaattcat cttctagctt atgaatggca tgcgttaatg caggctgagc
     6421 tatacaaaga ttttttgcac ttttagtaat atgttcattt ttagccactt ctaaaaaata
     6481 acgtagatga cttaattcca taaaagaatc cataaataaa agttatgtat tcaatataaa
     6541 taatgtatta gaaattatca attgagtttt ttagctttga acacgttgaa gaaaaaggga
     6601 acgtgaaaac accctaagga gaacaaaatg tccaatgctc tagaagcgat gatgcttata
     6661 tgctttggcc tttcgtggcc gatttctgtt tacaaaaaca taaaatctca ctccgccaaa
     6721 aatatgagtt ttcgcttcaa tctactgatt atcataggct accttgcggg catactcgcc
     6781 aaatgttata tgcaagcttt taattatgtg cttgtagtat atgtggtgaa tctaatgatc
     6841 gtaagcatga atatggttgt ctatttcaca aacaaaaaat acgatgaaat ggcttatcaa
     6901 ggagaagtat atgccaaata aaaacataac cgttttattc attttaatga cgttgttgct
     6961 ttttgtaggt tgtaaagaca atcgttcttg caactatgat ccagaaacag aaacattaga
     7021 atgttcagaa gctacttata aaaccgcaca agttaatgga aaaacttggc tagcagaaaa
     7081 cttaaattgg cccgtggttg attcgagttt ctgctacgcc aacgattttg gaaattgtat
     7141 aaagtacgga aggctttata catggggtgc cgcgatgaac cttacaaata aaacttcaga
     7201 aaatccacaa aaaggaatat gcccacaagg gtggcgcctt ccaacatcac aagaatttga
     7261 atctgcatta aaatccgctg atggcaaaaa actcaatgtc attcgagctg gtttccgcta
     7321 taataacgat tcttatgtcg atgaaaataa aagtgcgagt ttttggactg cttctgagtt
     7381 tgattccaca agagcttatt tagtacgagt gcttgttgaa caaagcatgc ttgaacacta
     7441 cagcaaaaca attgcaagct ctattcgttg cgtgatggat taataaataa cgattgtaaa
     7501 agacgtctca tgagccctat tctataacaa taaatgttat agaatagttt tattttaaaa
     7561 cgataaaata tacagctaag agttggtgcg agttacgaat gaattaaaag tttatcgcaa
     7621 taaaagcaga agcggctcca aaattatggc taaaagaggt taaaccatcc caaaggatat
     7681 tgtaacgaag gcccaactct agttgcaaac ttgatttttg aaaaaagata acacctgcat
     7741 caacatcacc tgcaaaacca aaggcgaaac cttctttaaa tcgatcgaaa tgaccatctg
     7801 cttgaatacc aaaaccaaaa ccagcaccaa gataaggagc aatacttcca gtattaaaaa
     7861 gatacctgcc gccaatggtt aacacttctt gtacatggga atcatcgcca atattaaaaa
     7921 cggtgtagct ttgtaacaca agtgctgttg atgaagaatt ttcccaaata taaccgagac
     7981 gtaaggcgta agcactttcg aaatctcgag aaggattatg ttccttatct ttccagttat
     8041 gccaataggc aaaaccaaaa ccaacgcttg tgtatttacg agcaggtgtt ttttcgagat
     8101 aatcctcttc tgcttctttt tcttgtggtt gtgaagcaga agcttcttga gagcctgttt
     8161 tagaaccaag agcagaagcc actgcttgct caatggcaat atcaagatcg tctatgcttg
     8221 cggctttttg tttcccagag cctacaatca cacctttttt tgtttgttca caaacaacaa
     8281 taatagaaga acccatggtc atcaaattgg tggtgagctt tatagactcc tctttttctg
     8341 ttggcgtatg accagcttgg ctcaccgcag aacgaataat cgtagaaacc atattaggag
     8401 catcaggtga aaaatctgct ccagtagggg cgacaatttg aatatttgct gcaaaagaga
     8461 atgcaactag agaaagaata gagaaaagta ttttattcat gaagtaaaat ataatgaatt
     8521 tgaaaggagg ttggggagag taaatggttt tggagaggga aatttaggca aattaaagat
     8581 g
//
LOCUS       DAOOBB010000074         8507 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_10210_length_8507_cov_4.295078, whole genome shotgun sequence.
ACCESSION   DAOOBB010000074 DAOOBB010000000
VERSION     DAOOBB010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 8507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8507
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10210_length_8507_cov_4.295078"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1649
                     /locus_tag="PLT31_04780"
     CDS             <1..1649
                     /locus_tag="PLT31_04780"
                     /inference="COORDINATES: protein motif:HMM:NF015387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HPW94488.1"
                     /translation="GYYTNSYLVAYDFDGSKLTQRWYHKSEKSGQGLYGEGNHNLSVG
                     DVNGDGFDEIIMGAAALKHDGTLLYRTGFGHGDAMHLSDMDPDKAGLEVWSVHENKAS
                     AYGFELRGPTGSVIFGKKTGTDNGRGLAADIDSTHRGFEMWSSSDASVFNVKGQSIST
                     NKPSVNFRIYWDGDLYDELLDGTKLDKWNGNGTTRLFTAYNFNNSKEINGTKANPNIS
                     ADLFGDWREEIVYYNSSNPAQINIFTTTIPSPHRVYTLMHDPVYRLSIAWQNVAYNQP
                     PHLGYFLPDAVKKGITAPDVYLVASNKIPNPGSSSSSTPSSSSSSIVSNVLSVVNAAY
                     PEEGDGIFENTNTGFTQDGYYNFTNSTESFGTWLLYSPKDATAATLSITYANGGTASR
                     DMVLMLNETSLGTVSFPSTGSWTTWTTVTKSITLKNGKNELKLTSTTADGGPNIDVFG
                     FSEAGIILYKDITSNHRNIYAVDSYQPKTGILKVSSNGLVKIDVFDMLGNKVLSSTKD
                     VIAGESMIPLNSSQLSKGIYWVQVRFNNKVLSRSKIGVTR"
     gene            1761..3563
                     /locus_tag="PLT31_04785"
     CDS             1761..3563
                     /locus_tag="PLT31_04785"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HPW94489.1"
                     /translation="MKKKEYLVFSLITVFLLCLNTSLWAKITIYMCGDSTMQDWALGY
                     YPKQGMGQDFSYFFDGAFIGVYNAGRGGTTSQTYYDGHWSQSFTKNGVTYPAVSSLIK
                     SGDYVFIMFGANDNGYKTGEVNFKNSIGSMVKQTQAKGAYPILFSPIRRSNFTSPDSV
                     YESYHAYPIFMRQVADSLKVPLIDLDTLSRNLLISLGQYYSNHYINMFLDAGEYSNYA
                     SAQTDNQHLQQSGANAMGRIVTEQLRVHSDANAKKLAQYLAPMYQVDVKVSPVGSDSV
                     TSVSTYYPKGMTVTLKTTPKNGKKFLGWYNGQGQKVSGNSMSQVKSSYIHTFVMGSAS
                     TQYTAVYEGGQAELYTGDGKALTAFPSTTPKKLGQGTITPTSSSSFVEVSSSSVQKVE
                     LLSTFDAFKPDTGIGYSESNNAGFHGEGFWNFQNQKEAFANYKMVFPSAGFVTMGIIY
                     ANGGANDRSLNIYLDHDYYVSFPSTGSWTTWDTVFVDLDLMNGEGDLQFISMTEEGGP
                     NIDMFGFSMENINRENQIPTKIKTSPFQIAKEEPIQVSVFDLQGNLLVEKMLSSYAED
                     YSLRVREWITQRGTFYVMVRQKSKVISSKQVQVK"
     gene            3633..5360
                     /locus_tag="PLT31_04790"
     CDS             3633..5360
                     /locus_tag="PLT31_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HPW94490.1"
                     /translation="MATVAITDSTSFTIHVIGDSTVATYKSSVYPQTGWGQVVGSFFN
                     ESQVLINNVAIGGRSSKTFFTDGRLAALKSQVKPGDYLFVQFGHNDRYFGSNARQVPL
                     DSFAYFLKIYLDSARSWNAIPVLVSPMVMNAWASNGTMRNVFTENGSDYRGVMEKLAK
                     EQDVAFVDLNMRSFEDCKKWGQTYISSFLYHHYDVGDYPNWPQGSSDGTHFQENGSVS
                     NARWIANEISSLSSSTYLSNATKQKLLPLIDALKPHYEVTVKTNKNVSGLVTKTALFP
                     AGAPLTLRVKPASGETFEYWADADCRQVSTSQIYYGIQAPGKPTTYTAMFKGGSACVQ
                     ASVPSSSSNEVSSSSSVEVSSSSVIVIRPEILSWVDMANPDSGSGTTDVNHEGYTGEG
                     FWNIENALGSKAFYQLESKNASTNAVMAIRYANGSTETRSMKVTLDGWTYSVAFPPTE
                     TWATWDTIFVEDVWIDAVSMPLVIESTSETGGPNIDALGLSVLGLTRESSITSIEVIS
                     KNKSLLFSNNNPVQILLFDALGTCFLNQQFSSFEYENFMSSLAPGVYYIKGIQEEKTF
                     FEQRVTKKK"
     gene            5494..6396
                     /locus_tag="PLT31_04795"
     CDS             5494..6396
                     /locus_tag="PLT31_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02147 family protein"
                     /protein_id="HPW94491.1"
                     /translation="MSIPIFEYLNYREFLRDAYEKKHSEDWHFSHRYIASCARMDASM
                     FNKILQGKRNLTPRLIDVFASLFCDTVEEKEYFINMVAFNQAKTHSDSRLYLERLVAH
                     KECKVHSLAKDQFEYFNHWYHAVVRELLVIYPYSGDARSFGQMIFPPIKASQVKESIA
                     LLERLSLIKKNESTGLYEQTEGFVTSGSESYSTEVNHYIQQNLKIACTALDRFNSKER
                     NLSTLVFSCDEESYGEVVEMVRRFRREVLSRISASKKSNRVFQLGIQFFPLSDTYPPP
                     IRRGRKKKISDKEQNNRDMEVDCD"
     gene            6389..8500
                     /locus_tag="PLT31_04800"
     CDS             6389..8500
                     /locus_tag="PLT31_04800"
                     /inference="COORDINATES: protein motif:HMM:NF012754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPW94492.1"
                     /translation="MIKGFWGVLFLSLLCISCSDQVAGGTEAESTIALEVISPSGTLA
                     SYARARFLPSSYLSNGEPSSEWVKADHEGKIEYKTLDTGAFTIEVRYLEESESFGAIK
                     TMSVVPGKKVFIDSLSLKQLATIEGSITPGQGPSVIRIAGLERFIVPDSAGYFVVDSL
                     PLGDFELVIESRSNRGDLKVEAETGEVLLDLELGDAKGFAVEDFESFSGVSQTGLILG
                     DGWWYTLDKSQENIKPLWDETLIRSYSGRVGCVSGGCARTSDHLGFLIGLWDTDYNLS
                     SLDTLYFAAKGNGSLNIALAYGEYGENESGLKTTVPLEENWKGYAVAVSDMIPYGSAK
                     EDTILVSRIHFSVSGGGEVFLDDISLGKIDSTFLENAKVEWEKPEASVFPGEDWGEHD
                     ALLSQIVGYGALTKGGSAGTICLVTTEDDLVLNEEGNYIVAEGSLRECAMKEGPVWIQ
                     FQKDGIYRLLAPLRIRSYKTVDGRGRDVRIAGMGILADSVSNLVFENLRFTSPAITEE
                     DSSSRRALSIHNKSTNVWVDHCTFEDYPLVLLDIKRGSDSVTISWSRFENSTHAILLG
                     LSPVTFFDSLEHLTAHHNYFTGLKGVGILNHGNNGHYYNNFFMSNGTVGVACTDRARC
                     LLENNIFNVPTPIYNIRVDSFGSIVSGTEGYIHMIDNWLAEGGETMQSANKVPLPSYD
                     YTVEEMDPALALRVQRDTGPR"
BASE COUNT         2521 a         1442 c         1806 g         2738 t
ORIGIN      
        1 gaggatatta cactaattct tatttagtgg cttatgattt tgatggctct aaattaacac
       61 aacgttggta tcataaaagt gaaaaatcag gtcaaggtct ttatggagaa ggaaaccata
      121 atttaagtgt aggtgatgtc aatggtgacg gctttgatga aattattatg ggtgctgctg
      181 cattaaaaca tgatgggact ctgttgtatc gtactggatt tgggcacggg gatgcaatgc
      241 atttgtctga tatggatcca gataaagcgg gtttagaagt atggagcgtt catgaaaaca
      301 aagcttctgc ttatggtttt gaattaagag gcccaacagg ctcagttatt tttggtaaaa
      361 aaacagggac tgataatggt cgcggtcttg ctgccgatat tgattctaca catcgtgggt
      421 ttgaaatgtg gagctcttcc gatgctagtg tttttaatgt gaagggacaa tcgatttcta
      481 ccaacaaacc ttccgtcaac ttccgcattt attgggatgg tgatctttac gatgaattac
      541 tcgatggtac aaaattagat aaatggaatg gtaatggcac aacgcgtctt tttacagcct
      601 acaatttcaa taactctaaa gaaattaacg gtactaaagc taaccccaat atttcggcag
      661 atttgtttgg tgactggcgt gaagaaatag tttattataa tagcagtaat cccgctcaaa
      721 tcaatatctt cactactacg attccttctc ctcaccgcgt atacacctta atgcacgatc
      781 ctgtatatcg tttgtcgatt gcttggcaaa atgtggctta caatcaacca cctcatttag
      841 gttatttttt accagatgcc gttaaaaagg gaatcacagc tccagatgtt tacttagtgg
      901 cttcaaataa aataccaaat ccaggttctt catcgagttc tactccttca tcgagttctt
      961 cttccattgt aagtaatgtt ttgtcggttg tgaatgccgc gtatcctgaa gaaggcgatg
     1021 gtatttttga aaatacaaat acgggcttta ctcaagatgg ttattataat ttcacaaatt
     1081 ctacagagag ctttggaact tggcttctct attctccaaa agacgccaca gccgccacgc
     1141 tttctattac ctatgctaat ggaggcaccg cttctcggga tatggtttta atgcttaatg
     1201 aaacgagttt agggacagtt tcttttccat ctacagggag ttggactact tggactacag
     1261 ttacaaaaag cattacactt aagaatggta aaaatgaatt gaaattaaca tcgactacag
     1321 cggatggcgg gccaaatatt gatgtgttcg gctttagtga agcaggaatt attttatata
     1381 aagacatcac ttccaatcat cgaaatattt atgccgttga ttcttatcaa cctaaaacgg
     1441 ggatattaaa agtttcatct aatggcttgg taaaaataga tgtctttgat atgctaggaa
     1501 ataaagtgct ttcttcaaca aaagatgtca ttgctggaga aagtatgatt ccattgaatt
     1561 caagtcaatt atcgaaagga atctattggg ttcaagtgcg ctttaataat aaagttcttt
     1621 cacgtagtaa gatcggggtt actcgttaat ctgatccgtt agtgatgttt cgagtgtttc
     1681 atctttaaaa cgggtgcaca tcctccgaga acacaataaa atgtgttctc ggagtttttc
     1741 atagggtata aggcttgggt atgaaaaaaa aagagtattt ggtttttagt cttataacag
     1801 tgtttttgct ttgcttaaat acaagtcttt gggctaaaat aacgatttat atgtgtggtg
     1861 attctaccat gcaagattgg gctttaggct attaccctaa acaagggatg ggacaggatt
     1921 tctcttactt ttttgatggc gcttttattg gcgtttataa tgctggccgc ggaggaacca
     1981 cttctcagac ttattacgac gggcattgga gtcagagctt caccaaaaat ggagtcactt
     2041 atcctgctgt ttctagtctc attaaaagtg gcgattatgt attcatcatg tttggtgcca
     2101 acgacaacgg ttataaaaca ggtgaagtga atttcaaaaa tagtattggc tcaatggtta
     2161 agcaaactca agccaagggt gcttatccta ttttattttc tcctattcgt cgttctaatt
     2221 ttacaagtcc agattctgtc tatgaatcgt atcatgcata ccctattttt atgcgtcagg
     2281 tcgccgattc tttgaaagtg cctttaattg atttagatac gttatctcga aatttattga
     2341 tttctcttgg acagtattat tctaaccact acatcaatat gtttttagat gcgggggaat
     2401 attctaatta cgcttcagca caaacggaca atcagcattt acaacaaagt ggagcaaatg
     2461 ctatggggcg tattgtcaca gaacagcttc gagtgcattc agatgcgaat gcaaaaaaac
     2521 tcgctcagta tttggcgcct atgtatcaag tcgatgtgaa agtaagccct gttggctcag
     2581 attctgtaac ttcggtaagt acttattatc caaagggaat gacggtcact ttaaaaacaa
     2641 cgccaaagaa tggtaaaaag tttttgggct ggtataatgg gcaagggcaa aaagtcagtg
     2701 gcaacagcat gagtcaagtg aaatcctcct atattcatac ttttgtgatg ggttccgctt
     2761 ccactcaata tacagccgtt tatgaaggag gacaagcaga gttatatact ggcgatggaa
     2821 aagccttgac ggctttccca tctactacgc caaaaaaact tgggcaaggg actattacgc
     2881 caactagctc tagttctttt gtggaggtga gttcttcctc agttcaaaag gtagaattgc
     2941 taagtacttt tgatgccttt aagccagata caggaatcgg gtattctgaa tcgaataatg
     3001 caggatttca tggcgaaggc ttttggaatt tccaaaatca aaaggaagct tttgcaaatt
     3061 acaaaatggt gtttccaagt gctggttttg tgacaatggg gattatctat gccaatggcg
     3121 gtgcaaatga tagatctctt aatatttatt tagatcatga ttattatgtg agctttcctt
     3181 ctacaggaag ctggactact tgggatactg tttttgtgga tcttgattta atgaatggag
     3241 aaggcgatct tcaatttatt tctatgacag aagagggcgg gccaaatatt gatatgtttg
     3301 gttttagtat ggaaaatatt aatcgagaaa atcagattcc tactaaaatt aaaacctctc
     3361 catttcaaat tgcaaaagaa gaaccaattc aagtttctgt ttttgatttg caaggaaatc
     3421 tccttgttga aaaaatgctg agttcttatg cagaggatta ttctttgcgc gtaagagaat
     3481 ggattactca gagaggaacc ttctatgtga tggttcgtca aaaatcgaaa gtgattagct
     3541 ctaaacaagt tcaagtaaag taaagaacgt tcagtagaaa ggcaattttg tatataaaaa
     3601 aaacatttag tactttattt atccttttag tgatggctac agtagcgatt acggattcga
     3661 cgtcttttac aattcatgtg attggcgatt ctaccgtggc cacttataaa agttctgttt
     3721 acccacaaac aggatgggga caagtcgttg ggtcattttt taatgagagc caagttttaa
     3781 ttaataatgt agcgattggt ggccgaagct ctaaaacatt tttcacagat ggtcgcttag
     3841 ctgctctaaa atcacaagtg aaaccaggag attatctctt tgttcagttt ggtcataatg
     3901 atcgctattt tgggtctaac gcacgtcaag tgccattaga ttcttttgcg tatttcttaa
     3961 aaatctattt agattctgca agaagttgga atgccattcc agtacttgtc tctccgatgg
     4021 ttatgaatgc gtgggcaagt aatggaacga tgcgaaatgt attcactgaa aatggttcag
     4081 attatcgtgg cgtcatggaa aaactcgcga aagaacaaga tgtggcattt gttgatttaa
     4141 atatgagatc ttttgaagat tgtaaaaaat ggggacaaac ttatatttct tctttccttt
     4201 accatcatta tgatgtgggt gattacccga attggcctca aggctcaagc gatggcactc
     4261 attttcagga aaatggttct gtaagtaatg caagatggat tgctaatgag atttcttcac
     4321 tttcttcatc tacctattta tcaaatgcaa caaaacaaaa gcttttacct ttaattgatg
     4381 ccttaaaacc acattatgag gtgactgtaa aaaccaataa aaatgtttct ggtcttgtaa
     4441 ctaaaacggc cttgttccct gcaggggctc ctttaacatt acgcgttaaa cctgcttctg
     4501 gagaaacttt tgaatattgg gccgatgctg attgccgtca ggtttctaca tcacaaatat
     4561 attatggcat tcaagctcca ggaaaaccaa cgacttatac cgccatgttt aagggaggct
     4621 ccgcttgtgt acaagcttct gtgccttctt cttcaagcaa tgaagtgagt tcttcaagta
     4681 gcgtagaagt ctcttcaagt agtgtgatag taatccgtcc agaaattttg tcttgggtgg
     4741 atatggcaaa tccagattct ggttctggaa ctacagatgt aaatcatgaa gggtatacgg
     4801 gtgaaggttt ttggaatatt gaaaatgctt tgggtagtaa agctttttat caattagaat
     4861 caaaaaatgc ttctacaaat gcagtcatgg ctattcgtta cgcgaatggt tctactgaaa
     4921 caagaagtat gaaagtaacc ttagatggat ggacttattc agttgctttc ccaccaacag
     4981 aaacctgggc cacgtgggac accatatttg tagaggacgt gtggattgat gctgtttcga
     5041 tgccattagt gattgaatcg acgagtgaaa ctggtgggcc aaatattgat gctttagggc
     5101 taagtgtact cggtttaaca agagaatcat ctattacgtc aattgaagtg atttcaaaaa
     5161 acaaatctct attattctca aataataatc cagttcaaat tcttttattt gatgctcttg
     5221 gaacttgttt tctaaatcaa caattttctt cttttgaata tgaaaacttc atgagctcac
     5281 tcgctccagg agtttattat atcaagggaa ttcaagagga aaaaaccttt tttgaacaaa
     5341 gagtgactaa gaaaaagtaa cggagtagtt taaaatctgt tatagagtaa ggtgaatgtg
     5401 ttttagaaca aagaaaagca ttctatactg tttttttatg tgaatgagtc gttttatttt
     5461 ttattttttt gactattgat ccaaaggttt aaaatgtcga tccctatttt tgagtactta
     5521 aattatcgcg aattcttaag agatgcttat gaaaaaaaac actctgaaga ttggcatttt
     5581 agtcatcgct atatagcgag ttgtgctcgt atggacgcct cgatgtttaa taagattttg
     5641 caaggcaagc gaaatctaac gccacgtttg atagatgttt ttgcgtctct tttttgtgac
     5701 actgtagaag aaaaagaata cttcattaac atggtggctt ttaatcaagc caaaacccat
     5761 tccgatagtc gtttgtattt agagcgttta gttgctcata aagaatgtaa ggttcattct
     5821 ttagcaaaag accagtttga atattttaat cattggtatc atgcggtggt tcgagaatta
     5881 ctggtgatat acccttatag tggggatgct cgttcctttg gtcagatgat atttcctcca
     5941 ataaaagcat cacaagtaaa agaatccatt gctttattag agcgtttgtc tttaattaaa
     6001 aagaacgaat ccacaggctt atacgaacaa acggaaggct ttgtgacgag tggttcagaa
     6061 tcttatagta cagaagtcaa tcactatatt caacaaaatc taaaaattgc ttgtaccgct
     6121 ttagatcgct ttaattctaa agaacgtaac ctttccacac ttgtattttc ttgcgacgaa
     6181 gaatcttacg gcgaagtcgt tgaaatggtt cgtcgatttc gcagagaagt gttaagccga
     6241 atcagtgctt caaaaaaatc gaatcgagtc tttcaattag ggatacaatt ttttcctttg
     6301 agtgacactt atcctccacc aatccgtcgt ggacgtaaaa aaaagataag tgataaggaa
     6361 caaaataatc gggatatgga ggtggattgt gattaagggt ttctggggtg ttctttttct
     6421 aagtctatta tgcatttctt gctcagacca agttgctggt ggtactgaag cggaatccac
     6481 tattgctctt gaagtaattt ctccgtcagg tacattggct tcttatgctc gtgctcgttt
     6541 tttaccttca agctatttat ctaatggaga accttcctct gaatgggtta aggcagatca
     6601 tgaagggaaa atagaataca agactttaga tacaggtgcg tttacgattg aagtacgtta
     6661 cttagaagaa tcggaatctt ttggagccat taaaacaatg agtgtggttc ctggtaaaaa
     6721 agtattcatc gattctcttt cgttaaaaca attagccacc attgaaggat ccattactcc
     6781 agggcaaggg ccttctgtaa tcagaatcgc tggtctagaa cggtttattg ttccagatag
     6841 tgctgggtat tttgttgtcg attctcttcc tcttggcgat tttgaattag tcattgaaag
     6901 tcgttcgaat aggggcgatc taaaagtaga agccgaaacg ggagaagtcc ttctcgattt
     6961 agaacttggg gatgcaaagg gttttgcagt agaagatttt gaatcattta gtggcgtttc
     7021 acaaacaggg cttattctcg gcgatggctg gtggtacact ctagataaat ctcaagaaaa
     7081 tatcaaacca ctttgggatg aaactttaat tcgaagctat tctggtcgcg tgggctgcgt
     7141 ctcgggcggt tgcgctcgca ctagtgatca tctaggattc ttaataggcc tttgggacac
     7201 cgactacaat ctttcctctc tagatacatt gtattttgca gcaaaaggaa atggaagttt
     7261 gaatatagca cttgcctatg gtgagtacgg cgaaaatgaa agtgggctta aaacaaccgt
     7321 accattagaa gaaaactgga agggttatgc tgttgccgtt tctgatatga ttccttatgg
     7381 tagtgcaaaa gaagatacca ttttagtcag tcgcattcac ttctccgttt cgggcggtgg
     7441 tgaagtcttt ttagatgata tttctcttgg aaaaattgat tctactttct tagaaaacgc
     7501 aaaagtagaa tgggaaaagc cagaagcctc tgtattccct ggtgaagact ggggagagca
     7561 cgatgccttg ctctctcaaa ttgtaggcta tggtgcttta actaagggtg gttcagcggg
     7621 aaccatttgt ttagtgacta cagaagatga tttagtgtta aatgaagaag gtaattatat
     7681 cgtagcagaa ggatctttac gtgaatgtgc tatgaaagaa ggacctgttt ggattcaatt
     7741 ccaaaaagat ggcatttatc gtttactagc tcctcttcgt atcagatctt ataaaacggt
     7801 tgatggtcgt gggcgagacg ttcgaattgc aggaatggga attcttgccg attccgtttc
     7861 taacttagtc tttgagaatc tccgttttac ttctccagcg attacggaag aggactcttc
     7921 ttctaggcgt gcactttcta ttcataacaa atccactaat gtatgggtgg atcactgtac
     7981 ctttgaagat tatccattgg tattattaga tatcaaaaga ggttctgatt cggtgactat
     8041 ttcttggtct cgttttgaaa atagcactca tgccatctta cttggtttaa gtccagtaac
     8101 tttttttgat tctctagaac acttaaccgc ccatcataat tactttacgg gcttaaaagg
     8161 ggtggggata ttaaaccatg gaaataatgg gcattattac aacaacttct ttatgagcaa
     8221 cggtactgtt ggcgttgctt gtacagatag agctcgttgt ttattagaaa acaatatttt
     8281 taacgttcct acgcctattt ataatataag agtggatagt tttggttcga tagtttctgg
     8341 tactgaaggg tatatccata tgatcgataa ttggcttgct gaaggaggag aaacaatgca
     8401 aagtgcaaat aaagtgccat tgccttctta tgattatact gtagaggaga tggatccagc
     8461 gttagcgctt cgggttcaaa gagatacagg gccaagataa tgcatta
//
LOCUS       DAOOBB010000075         8418 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_10312_length_8418_cov_3.622504, whole genome shotgun sequence.
ACCESSION   DAOOBB010000075 DAOOBB010000000
VERSION     DAOOBB010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 8418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8418
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10312_length_8418_cov_3.622504"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /locus_tag="PLT31_04805"
     CDS             complement(<1..1185)
                     /locus_tag="PLT31_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPW94493.1"
                     /translation="MSDQKLVPGTHVYSLIEKDMQDCYLRYSMSVIVARALPDVRDGL
                     KPVHRRVLFGMHDLGVYPGKPYKKSARIVGDVIGKYHPHGDFAVYETLVRMSQDFSLR
                     YPLVDGQGNFGSIDGDSAAAMRYTEARMTRFGEMMLEDLEKETVNYGPNYDDSLTEPL
                     VVPSSFPNLLVNGSTGIAVGMATSMAPHNLREITNAIHAVAENPDITGEELLQYVSGP
                     DFPTGGIICGRSGIREAYLTGRGKVRVRARVHTEEDAKGRSRIIITEIPYMVNKANLV
                     EKIADLVRDKRVEGISDIRDESDRSGMRVVIELKRDAVSEVILNNLYKYTQLQDTFSI
                     YNLALVNRTQPTLLTLKDLITSFIEHRIEVIRRRTEFDLKKAKARMHILEGLRIAQQN
                     IDE"
     gene            complement(1202..1969)
                     /gene="surE"
                     /locus_tag="PLT31_04810"
     CDS             complement(1202..1969)
                     /gene="surE"
                     /locus_tag="PLT31_04810"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HPW94494.1"
                     /translation="MQEKNRPHILITNDDGITSENLLVLVKALAPLCHVFVVAPDSEK
                     SGVSHAFTHREGLSCNRVFDYGFPAYAVSGTPADCIKFAVSELFKDQPIDLVFSGINV
                     GENAGVSSIYSGTVAAAREAALWKIPAVALSLQAPNEFMLQEIIAFAKAVVENKWYSK
                     IPAQTFWNVNFPDATVEKYQGIRATTMGLGMFTDYYECRDGLWYVDGCKIWEHALPGS
                     DDEAISLSFASLTPLSLNPTDHIELNRVQALFASVAQ"
     gene            2111..2833
                     /locus_tag="PLT31_04815"
     CDS             2111..2833
                     /locus_tag="PLT31_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HPW94495.1"
                     /translation="MSEIAKAKRFSEILRVIILQLGNDFQKAKQEYELYADWAELPME
                     KRIIGKNQAQLIDLYKTFRSEAGLGFERDVYLEQEPGDRETAINPPLPYSVLVHNLRS
                     AFNVGSIIRSVDCFGLEKVNLSGYSPEIDHVTLKSAARGAEGWIPTERWDSPMDCIEH
                     YKSLNYEIIALETGDQIANVADVSWPEKGLILVGNEELGIAPELMEQATLKVTIPMVG
                     RKASMNVAGAFAITAFCLRNKR"
     gene            complement(2976..3320)
                     /locus_tag="PLT31_04820"
     CDS             complement(2976..3320)
                     /locus_tag="PLT31_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94496.1"
                     /translation="MKKRLSNYLVILETVKKDSSLLATFDLQEMIVQIHFFQHERLIH
                     LLVTLFFGILTFLCVLYISLFPSIALFALNVLFFILLVFYLRHYYFLENNIQKLYSFY
                     DEIKKLKNEMHF"
     gene            3430..5193
                     /locus_tag="PLT31_04825"
     CDS             3430..5193
                     /locus_tag="PLT31_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPW94497.1"
                     /translation="MSLLYPSLCALFVENCKREDFPGWYLRKNGQWVFYSRETLRNHL
                     FYLSLALHKRGINSNNNLGIIAPTSPNWILADLATQMCHGITVPLFPNISSKNFLYQC
                     EDANVKFLIIENTTCLEKNLQETLSSFQLIISIDSSEPNPPNGIYWDDLLKEGRALAE
                     SKDMGDWIREQLHSISPDDIFSIIYTSGSTGRPKGVALSHRHMISQLRSVHSILDLNR
                     HNDSALSILPIAHVFERMTLYYYTLVGIKIYFADDPRNAATLLQEIKPTVMTVVPRIL
                     ERLYEKMIAAADHRSGINKLLIKKAIRLAKIQDPEKRSICKKIYEKLVYKKMRAAIGD
                     RFKYIISGSSALNKSINRFLLNIGLPVYEGYGLTECSPVVSFNYKNHSKIGSVGQKLN
                     HLEVKIGPQNEILVKGESVFNGYHNQEEYNRTIFTEDGFFKTGDEGYIDENGFLFIIG
                     RIKEMLKTSTGKYVSPLPIELELSRHPFIEAALVIANDRKYVSSLLFINFENTRRHLK
                     LSHEDFDPYKAIYSKRVQQAIERHIHNVNKKLNHWEQIQKWTLIADPLNIESGLLTPT
                     LKLRRKAAESIYHLDIEKMYS"
     gene            complement(5276..7603)
                     /locus_tag="PLT31_04830"
     CDS             complement(5276..7603)
                     /locus_tag="PLT31_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014544917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94498.1"
                     /translation="MLKRFFYAFILIDFILLLASCADDDEGVEYFFDREILDMSILRE
                     CASEADEGDYCYQIRFRYPIITSDLKGFHLWLDTTVIDDTSKAVPSAALEKSFFIEYD
                     EKKVDDFDTIDLTSMLVDYLDQDSLQVALWPEYTDKDRNEKKGSLQRLYIHFGDDIPP
                     SRVNFQDSVWSTGALFDWARPTDQTDFYAPNDISGPIVGYNIVIYAQNKEEDIRQVKV
                     KVLHDSHVDSTGSLLFQKHARIRSIDDSLWIDKTSQSDDVKNYLRIAVLDGKGFDFEN
                     TDANRFRIILEGLKPESRYTIGVSAWDSSGNSSGTDGVTTVENNQLFITTDKIAPLMS
                     KMIITNRDTLDPNLTRLDSNNRVRLFWSKSIDPIKEDHNIVEDSVLIIPSSCSEYFCY
                     KDVSKYIVERFNGISWDLLSYAGGEISSRYVKQYAWNNDSMIVSSIGQFVTDTIRWVL
                     PGDTLIIRIRSVDSSGYYSRALIDTLFIAPHVVADLDCPSGFIAVKTSDTTRFCMESI
                     EHRDAEGNFYKNVLHSEAMAICNGMALSGFTVSLCHERDWQQACLSGGSSTYGVIEDG
                     DVASSEYLFSYCNVGTNDSIFAKSFIDRNSKCANRYGIRDLPGQYQEWVLGRSPDTLQ
                     VLKGSSFRQFNGIDRETASMCTNRFFPFYTRMGYTQDSIYLYTNGTTVDTSLVEDTTK
                     TLYALLTKKDFKDTIQFFNVKSPETQEVIGEDYALLSEYKKGGSAWLEKLANGLIYEP
                     SRKEVVFLLKPTSYRAAAAFYKDNSIGFRCCAYPE"
     gene            complement(7667..7924)
                     /gene="rpmA"
                     /locus_tag="PLT31_04835"
     CDS             complement(7667..7924)
                     /gene="rpmA"
                     /locus_tag="PLT31_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPW94499.1"
                     /translation="MAHKKGQGSVHNGRDSNPKYLGVKKYGDEVVKAGNIIVRQRGSH
                     FHKGNNVGMGKDFTLFALIDGKVKFERLDSKRQKVSVYPEA"
     gene            complement(7927..>8418)
                     /gene="rplU"
                     /locus_tag="PLT31_04840"
     CDS             complement(7927..>8418)
                     /gene="rplU"
                     /locus_tag="PLT31_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HPW94500.1"
                     /translation="MYSIVETGGFQYKVELGKAYKVPSIAEAAVGSTLELKSVLLFSN
                     GKEVQVGTPVLSDATVKVEVLSHGKGDTVIVFKKKRRTRYERKNGHRQGYTEVLVTEI
                     CSGKESAAVDPQIITRNRARVAALAKQKAQNVPLTRKEKIAQGIPKPVKVKKNSLRKA
                     MEA"
BASE COUNT         2843 a         1582 c         1452 g         2541 t
ORIGIN      
        1 ttcatcaata ttttgttgag caatacgcaa gccttcgaga atatgcatgc gagcttttgc
       61 tttttttaga tcgaattcag tacgtctacg aatgacttca attcgatgtt caataaaaga
      121 tgtgattaaa tcttttagtg ttaaaagcgt tggctgagta cgattgacaa gagctaagtt
      181 gtaaatactg aatgtatctt gaagctgagt atacttataa agattattca aaatcacttc
      241 ggatacggca tcgcgtttta attcaataac gacacgcatt cccgaacggt cagattcatc
      301 acgtatatca ctaatacctt caacacgctt atcacgaact aaatcagcaa ttttttccac
      361 caaattggct ttattcacca tataaggaat ttctgtaatg ataatacgag atcttccctt
      421 ggcatcttct tctgtatgca ctcgagcacg gactctaact tttccacgac ccgttaaata
      481 ggcttcacga attccacttc ggccacagat aatgccacct gtagggaaat ctgggcctga
      541 aacgtattgc aaaagttctt caccagtaat gtctgggttt tcagcaaccg catgaatggc
      601 attggtaatt tctcttaaat tatgaggagc catagaggtt gccataccaa cagcaatacc
      661 agtagatccg ttcaccaaaa gattagggaa agaagaagga acaacaagag gttcagttaa
      721 agaatcatcg tagtttggtc cgtaatttac tgtctctttt tcaagatctt ctaacatcat
      781 ttcgccaaaa cgagtcatac gagcttcggt ataacgcatc gcagcagcag agtcaccatc
      841 aatagaaccg aagtttcctt gaccatcgac tagaggatat cgtaaagaga aatcttgaga
      901 catacgaaca agagtttcat agacggcaaa atcaccatgt ggatgatact taccaataac
      961 gtcacccacg atacgggcag actttttata cggtttccct ggataaacac caagatcatg
     1021 cattccaaaa agaacccttc gatgcacagg ctttaagccg tctcggacat caggcaaagc
     1081 tcgagccaca ataacgctca tcgaatagcg cagataacaa tcctgcatat ctttttctat
     1141 caacgaataa acgtgagttc ctgggactaa tttttgatct gacatttttt atctatcctt
     1201 tttattgagc tacagaggca aataaagcct gcactcgatt caattctata tgatccgttg
     1261 gatttaaaga aagtggcgta agagaagcaa atgacaagga gatggcttcg tcatcagagc
     1321 ctggtaaagc atgctcccaa attttacacc catcgacata ccacaacccg tctcgacatt
     1381 cataataatc tgtaaacata ccgagcccca tggttgttgc tcgaattcct tgatactttt
     1441 ctacagtagc atcagggaaa ttcacattcc aaaatgtttg agctggtatt ttagaatacc
     1501 atttattttc cactaccgct tttgcaaaag caatgatttc ttggagcata aattcatttg
     1561 gtgcttgaag agaaagggct acagcaggta ttttccaaag agcggcttct cttgcagcgg
     1621 ccacggtgcc tgaatagatc gaagacacac ccgcattttc acctacattg ataccagaaa
     1681 agacaagatc aatcggttga tctttgaata gttcactaac agcaaactta atacaatctg
     1741 ctggagtccc tgaaacagca taagcaggaa acccataatc aaaaactcgg ttgcaagata
     1801 aaccttcgcg atgagtaaaa gcatgactca caccactttt ttctgaatca ggagcaacca
     1861 caaaaacatg gcataaaggg gcaagagctt ttaccaatac gagcaaattt tcactcgtaa
     1921 taccatcgtc atttgttatt aaaatatggg gtcgattctt ctcttgcatt tgtagcctaa
     1981 aagatagaat ttatccactc aattagttgt atttttcaag taaaaaaagc gattttatgg
     2041 ataaaaaagt acattttaaa aaagcgtttt ctttaggatt tttatgtatc ccaaacacaa
     2101 atttttagcg atgagtgaaa tagcgaaagc aaagagattt tctgaaattt taagggtcat
     2161 tattcttcaa ttaggcaacg attttcaaaa agcaaagcaa gaatacgaat tatacgctga
     2221 ttgggcagaa cttcctatgg agaaaaggat tattggtaaa aatcaagcac aattaattga
     2281 tttatacaag acttttcgtt cagaagcagg tttaggtttt gaacgagacg tatatttaga
     2341 acaagagcct ggtgatcgag aaacggcaat aaatcctcca ctcccctatt ccgttttagt
     2401 acataatcta agaagcgctt ttaatgtggg gtccattata cgttccgtcg attgttttgg
     2461 gcttgaaaaa gtgaatttaa gcggttactc tccagaaatt gatcatgtga ccttaaaaag
     2521 tgcggcgaga ggtgctgaag gatggattcc tacagaacgt tgggattccc ctatggactg
     2581 tatagaacac tataagtcct taaattatga aatcattgct ttagaaacag gcgatcaaat
     2641 agcaaacgtc gctgatgttt cttggccaga aaaaggtcta attctagtcg ggaatgaaga
     2701 gcttggaatt gctcctgaat taatggagca agcaacgctt aaagtgacta tacctatggt
     2761 tggccgaaaa gcgagtatga acgtcgctgg agcttttgcg atcaccgcat tttgtttaag
     2821 aaataagcgt taaatccagg aaaggtaaac gagatacatt ttttgttgaa ttctacgaaa
     2881 gcaagctcca aaagcatctt tggtaaaaat caccgtaaag agtcttgtga aaaggacttc
     2941 aatccttgaa aagaacaccc acaaagaatg aactgttaga agtgcatttc gttttttagc
     3001 ttttttattt catcgtaaaa cgaatatagt ttttggatat tattttcaag aaaataatag
     3061 tggcgtaaat agaaaacgag aagaataaag aaaagaacat ttaaagcaaa taacgctata
     3121 gacgggaata gagagatata gagtacacat agaaaagtga gaatcccaaa aaatagcgtt
     3181 actaataaat gaatcaagcg ttcatgttga aaaaaatgga tttgaacaat catttcctga
     3241 agatcaaatg ttgcaagtaa agaagaatcc ttctttacag tctcaagaat caccaaataa
     3301 tttgatagtc tttttttcat agaactgatt ctttatgaag aaaaaatggt ttattacaat
     3361 aaaataactt tttttctttt attgatatta ttttaataga aacatgattg tattattttg
     3421 aggatccctt tgtctttact ctacccatct ctttgcgcac tttttgtcga aaattgtaaa
     3481 cgagaagatt ttcccgggtg gtatcttcgt aaaaatggac aatgggtatt ttattctcgc
     3541 gagaccttaa gaaatcacct tttctattta tctcttgctt tacacaaacg agggataaat
     3601 tcaaataata atttaggaat tatagccccc acctctccta attggattct tgccgattta
     3661 gcaactcaaa tgtgtcatgg gattacagtt cctttatttc caaatatttc atcgaaaaac
     3721 tttttatatc aatgtgaaga tgctaatgta aagtttttaa tcattgagaa tacaacttgt
     3781 ttagaaaaaa atcttcaaga aacgctttct tcttttcaat tgatcattag catagattca
     3841 agcgaaccaa acccacctaa tggaatatat tgggatgatc ttcttaaaga aggtcgtgca
     3901 cttgcagaat ccaaagacat gggtgattgg attagagaac aattacacag tatttctcct
     3961 gatgatattt tctccattat ttatacaagt ggttctacag gtaggccaaa aggtgttgct
     4021 ctatctcatc gccatatgat ttctcaatta agatctgttc attctattct tgatctaaat
     4081 cgccataatg attctgcatt gagcatttta cctattgcac atgtttttga aagaatgact
     4141 ctctactatt ataccctagt cggaataaaa atttattttg ccgatgatcc gagaaatgca
     4201 gccactctac tccaagaaat aaaacccacg gtaatgaccg tagtccctag aattctagaa
     4261 agattatatg aaaaaatgat cgctgctgca gatcatcgtt caggaataaa taaactacta
     4321 ataaaaaaag caatccgtct tgctaaaatt caagatcctg aaaagcgttc catttgtaaa
     4381 aaaatttacg aaaaactagt ctataaaaaa atgcgtgctg caataggaga tagatttaaa
     4441 tacatcattt ctggtagcag tgccttaaac aaaagcatca atcgattttt attaaatata
     4501 ggccttcctg tatatgaagg ttacggtctt actgaatgct ctcctgtagt cagttttaat
     4561 tataaaaatc attcaaaaat agggtctgtt gggcaaaaat tgaatcatct agaggtaaaa
     4621 attgggcctc aaaatgaaat tttagttaaa ggagaaagtg tttttaacgg ataccataat
     4681 caagaagaat ataatcgtac catttttacc gaagatggct tttttaaaac tggtgatgaa
     4741 ggttatatcg atgaaaatgg ttttctcttt attattggtc gaataaaaga aatgttaaaa
     4801 acatctactg gtaaatatgt ttccccttta cctatagaac ttgaactttc aagacatcct
     4861 tttattgaag cggcattagt gatagctaat gacagaaaat acgtatcttc tttattattt
     4921 ataaattttg aaaatacgag gcgtcatcta aaactaagcc atgaagactt tgatccttac
     4981 aaagctattt attctaaaag agttcagcaa gccattgaac gacacattca taatgtgaat
     5041 aagaaattaa atcattggga acaaatacaa aaatggacat taattgcaga tccattaaat
     5101 attgaatcag gcttattaac tcctacctta aagctaagaa gaaaagcagc tgaatcgatt
     5161 tatcatttag atatcgaaaa aatgtattct taaaaaacaa gcaaaacttt tattcttcaa
     5221 aaacacaaat aaaaattctg tattttataa atagtttata aaaaaactga aaagcttact
     5281 caggatacgc acagcaccta aagccaatcg aattgtcttt atagaacgct gctgctgctc
     5341 gatacgaagt tggttttaaa aggaagacga cttcttttct ggatggttca taaattaatc
     5401 cattcgctag cttttcaagc caagcggaac cacctttttt gtattcagaa agcaaagcat
     5461 aatcttctcc aattacttct tgtgtttctg ggcttttcac gttgaaaaac tggatagtat
     5521 ctttaaaatc tttttttgtt aaaagagcat acaacgtctt tgtggtatct tcaactaaac
     5581 tagtatccac agtggttcca ttagtataga gatatatcga gtcttgagta taacccattc
     5641 gcgtataaaa agggaagaaa cgattagtac acatcgatgc cgtttcacga tcaatcccat
     5701 taaattgacg gaaacttgag ccctttaaaa cttgaagagt atcaggagaa cgccctaaaa
     5761 cccattcttg atactgacca ggtaaatctc gaattccata acgattagcg cattttgaat
     5821 ttctatctat aaaagacttt gcaaaaatgg aatcattagt tcctacatta cagtaagaga
     5881 aaagatattc agacgaagcg acatcaccat cttcaatcac tccatatgta gaggagccgc
     5941 ctgaaagaca cgcttgttgc caatctcgtt catgacataa tgatacagta aatccagata
     6001 acgccattcc attacatata gccatggctt cagagtgcaa tacgttttta tagaaattcc
     6061 cttctgcatc tcgatgttct atagattcca tacaaaagcg tgtcgtatct gatgtcttaa
     6121 cagcaataaa accagaagga caatccaaat cggcgactac atgaggagca ataaaaagcg
     6181 tatcaattaa agctctggaa taatacccag aagaatccac agaacgaatt cttataatca
     6241 aagtatctcc aggcaacacc caacgaatcg tatctgtaac aaattgacca atactagata
     6301 caatcataga atcgttattc catgcatatt gctttacata acgagaagat atttccccgc
     6361 ctgcatatga caataaatcc caagaaattc cattaaaccg ttcgacgata tattttgata
     6421 catctttata acagaaatat tctgaacaag aagaaggaat tattaaaaca gaatcctcaa
     6481 caatattatg atcttctttt attgggtcga tgcttttgct ccaaaataaa cgaactctat
     6541 tattactatc gagtctagtt aaatttggat caagagtatc gcgatttgta ataatcattt
     6601 ttgacattaa aggggcaatt ttatctgtcg tgataaaaag ttggttattt tccacagtcg
     6661 tcacaccgtc tgtgccagag ctattacctg aggaatccca agctgaaact ccaatagtat
     6721 aacgactttc tggttttaag ccttctaaaa ttatacgaaa tctatttgca tcagtatttt
     6781 caaagtcaaa tccttttccg tctaaaacag ctattcttaa ataattttta acgtcatcag
     6841 attgacttgt tttatctatc cataaagaat catcaataga acgtatacgg gcatgctttt
     6901 gaaataataa agaaccagta gaatcgacat gagaatcatg aagtacttta actttgactt
     6961 gacgtatatc ttcttcttta ttttgggcgt aaataacaat attataaccg actattggac
     7021 ctgaaatatc atttggagca taaaagtctg tttgatctgt tggacgagcc caatcaaaaa
     7081 gagctcctgt agaccataca gaatcttgaa agttcactct tgatggtggt atatcgtctc
     7141 caaaatgaat atagagacgt tgcaaagagc ctttcttttc attacgatct ttatctgtat
     7201 attctggcca tagggcaact tgtaaactat cttgatctaa ataatccaca agcatggatg
     7261 ttaaatcaat agtatcaaaa tcatctactt tcttttcatc atattcaata aaaaatgatt
     7321 tttctaaagc agcagatgga acggcttttg aagtatcatc aataacagtg gtatcaagcc
     7381 ataaatgaaa gccttttaag tcactagtta ttattggata acggaatcgt atttgataac
     7441 aataatcacc ttcatctgct tcagatgcac actctctaag aatagacata tcgagaattt
     7501 cacgatcaaa aaaatactcg acaccttcat catcgtcagc acaagacgct aataagagaa
     7561 taaaatcaat taaaataaaa gcataaaaaa aacgttttag cattacagta taaaatacaa
     7621 aaaaaccgac tggaacacag tcggtttttt tgataaagcc ttgaaattaa gcctcgggat
     7681 aaaccgaaac tttttgacgc ttagagtcga gacgctcaaa cttcactttg ccatcaatta
     7741 aggcgaataa ggtgaaatcc ttacccatac ctacattgtt gcctttgtga aaatgagaac
     7801 cacgttgacg aacaataata ttgcccgctt taactacttc atcgccgtac ttcttgacac
     7861 caagatattt ggggttagag tcacgaccat tatgaacgga tccttgtcct ttcttatgtg
     7921 ccatggttaa gcctccatcg ctttacgaag tgaatttttc ttgactttca caggctttgg
     7981 aatgccttga gcgatttttt ctttacgagt taaaggcacg ttttgagcct tttgtttcgc
     8041 aagagccgca acacgagcgc gattacgggt aatgatttga gggtcaacag ctgcagattc
     8101 cttgccagag caaatttccg taacaagcac ttcggtatag ccctgacgat gaccgttttt
     8161 gcgttcgtaa cgagtacgac gttttttctt aaaaacgatc actgtgtcgc cttttccgtg
     8221 agaaagtact tctactttca cggtagcgtc actcaggaca ggggtgccga cttgcacttc
     8281 ttttccgttt gagaaaagaa gaacggactt gagctccagt gtagaaccga cagcagcttc
     8341 agcgatgcta ggaaccttat aggctttgcc taactcgact ttatactgga aaccacctgt
     8401 ttcaacaata gaatacat
//
LOCUS       DAOOBB010000076         8396 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_10343_length_8396_cov_3.558926, whole genome shotgun sequence.
ACCESSION   DAOOBB010000076 DAOOBB010000000
VERSION     DAOOBB010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 8396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8396
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10343_length_8396_cov_3.558926"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..609)
                     /locus_tag="PLT31_04845"
     CDS             complement(10..609)
                     /locus_tag="PLT31_04845"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HPW94501.1"
                     /translation="MFTGIIQATGKILSLEWRGDALSMRLFASQFFKDSKVGDSVANN
                     GVCLTIENCTEDEATFCLIHQTVKNTSFIDTKVGDLVNLELPCTPQSLMGGHFVMGHV
                     DSAIKVHSRVERETGIEVELEIPSDLRRYIIRRGSVTLNGISLTVAEKTELGIVVAII
                     PETLLKTNLATWQTGTLVNIEVDMIGKYVENYLKEAKLA"
     gene            complement(838..1608)
                     /locus_tag="PLT31_04850"
     CDS             complement(838..1608)
                     /locus_tag="PLT31_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HPW94502.1"
                     /translation="MNLTLKIWRQKDSKSKGQFETVKIGGISPDMSFLEMLDTVNEQQ
                     MKEGKEGFAFDHDCREGICGMCSLVINGMPHGPDHAITTCQLHMRKFKDGDTIVIEPW
                     RASAFPVIRDCAVDRSAFDRIIQAGGFVSVNTGSAPDAATIAVPKDQADRAFDAAACV
                     GCGACVAACKNSSAMLFVAAKVSHLSYLPQGKVEAKKRVLAMVAQMDKEGFGNCSNLY
                     ECQAACPKSITVDFIAKMNREYLGATALYAEKVYGEDM"
     gene            complement(1620..3533)
                     /locus_tag="PLT31_04855"
     CDS             complement(1620..3533)
                     /locus_tag="PLT31_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HPW94503.1"
                     /translation="MILDSKIPGGPISEKWTRHKFELKLVNPANKRKFTVIVVGTGLA
                     GASAAASLGELGYNVKAFCIQDSPRRAHSIAAQGGINAAKNYTNDGDSVYRLFYDTVK
                     GGDFRAREANVHRLAECSNLIIDQCVAQGVPFGRDYGGLLDNRSFGGAQVSRTFYARG
                     QTGQQLLIGAYQAMMRQVAKGTVKMFPRRDMQDLVVIDGKARGIIVRNLVTGELESHE
                     ADAVCLCSGGYGNVFYLSTNAKGSNVSAAWSAYKKGAVFANPCYTQIHPTCIPVTGDH
                     QSKLTLMSESLRNDGRVWVPKKAGDTRSPDQIPESERFYYLEEKYPSFGNLVPRDVAS
                     RNAKNVCDNGMGVGQSKLAVYLDFSDAIKRLGVSAVSAKYGNLFQMYEKITDENPYKV
                     PMRIFPAVHYTMGGLWVDYNLMSTIPGCFVLGEANFSDHGANRLGASALMQGLADGYF
                     VIPYTIGGYFASTKLEKISSTEKEFEVSKKESSERIHKLLNIKGKRTVSDIHRELGHI
                     MWENVGMGRNKAGLEKALALIPELREEFWKNVNVLGSEGSFNQNLEHASRLADFLEFG
                     ELMAQDALHREESCGGHFREESQTEEGEAKRDDEKFCYVGAWEYKGNNQKPELSKEPL
                     TFENVHLVTRSYK"
     gene            complement(3533..4396)
                     /locus_tag="PLT31_04860"
     CDS             complement(3533..4396)
                     /locus_tag="PLT31_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HPW94504.1"
                     /translation="MEWIIKYITSSIGKKQIMGCTGAILAMFILGHMVGNIQLINLDP
                     LAAQASYNAYCELLTGHKAFIYTVEAVMMAVFILHVFLAVTLKLQNKKARGGSDYDVN
                     ARKGKKTFATFTMIWSGLFVVFFLVSHLMSLKFGEYFFYQSPDVADGRVIRDMWLTTI
                     IAFADIKFSAFYVVSMFIVGLHLFHAISSAFQTMGIAHQKWTPFIELLGIVYSVVVAL
                     GFALIAVASFILGNKPETQDLIEKSRDIGKQKMLEEQKKMEEAEKGAFILPSSIENTQ
                     VSYVFEMNGGR"
     gene            complement(4424..4882)
                     /gene="ndk"
                     /locus_tag="PLT31_04865"
     CDS             complement(4424..4882)
                     /gene="ndk"
                     /locus_tag="PLT31_04865"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HPW94505.1"
                     /translation="MEMTFAMIKPNGVASGLVGRIIERYESARLSVCAIKMHQMTSAD
                     ARGFYAEHVEKPFFGELEEYMTKGPSVMLVLGGENAVAKVRAINGATNPAKSEPGTIR
                     HDFAPSMTENVVHSSDGVESAAREIAFWFEKENIYAYDAASQKACCVMAK"
     gene            complement(4961..7384)
                     /locus_tag="PLT31_04870"
     CDS             complement(4961..7384)
                     /locus_tag="PLT31_04870"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPW94506.1"
                     /translation="MNSKKILLLSFTLLSLVGCNSLGTKEELVARIGSEKVYLSDVEL
                     SQKLANAEKKSARFADIFNQVILNEGMAAVARTTYPGIASKVDDDLKRMDNRLMTMVY
                     QQYHVLEMFGFKQSEVEKYYEANKDSFPMDSTQTFNDIRKSVAQKLFIEANADSVNRF
                     IEQNLSNFSEPALAELYFFKSDTKKESSKIESAILAKTPIDSIKGVNRTVVNEKIYHE
                     LTALKELKPFIFGDSALPVDSVPKTIAVVDSLNDSTFYTVQMISRKETKAAVLEEHLA
                     DLHRMFIDNYTRDMMRESYRRFEKKYDVVKQPISDAEAKKYYDSHIELYKTLPGYSLY
                     HIEHSDSAILKKDVLDQVSSLDDFKKKATELSQNTFTKEQEGLVGSVKKSHSMPYGIG
                     LVPQVFDEFTGKPAGTISSIIKAPKTQKYHVFYLEKEIPAEPKSFDRVRSTVLNEIAN
                     DDNLKLDSSFVLVTAQGKPLVRESDLIALRNEIPESQRVAFNRTRLIDFLTQWAVYAM
                     EAKSFDLDQSWEYKAFVRQTRRDLTNQYFKDSLRLKKEFSDEDLKTVFDQVGAKIAPT
                     ATFEELIPQLKIYLKTPEIVLKREYYFNMDAYRSFADFEAARGMVFRNISSIEESNQW
                     KRLERDMWSKYKVTVFNSKMPALKTIFSSDSLFLEAEQFYNNRKLNEARANYELIRSL
                     YPDNEAAYKKATFEIATIDNENESYNNAESEYRVYYSLWPTDPNSEKALFSRAFVLSE
                     NLKNDSLALPLFKDFITKYPKSELKESVEWLIKNIESNGKLAQELVEKISKIEETDSL
                     GSISDKKAE"
     gene            7500..>8396
                     /locus_tag="PLT31_04875"
     CDS             7500..>8396
                     /locus_tag="PLT31_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94507.1"
                     /translation="MSKETDPQHQSSKNKNWSRRLIAVAFFAIFVSSVVFTAWTLFYW
                     LDHFFKEKQTYGSVVVEPFQYSRDHILSHHWDSILVYSPDFSISIQKPHFRIFDLLSS
                     KRSALLKVEHVKCHFSTQPKDSTSAPFSLDSVKIPNIKIPIPVFIELDSSLVEVDSTL
                     FWKTQGLKINNESANNVILSIDTIDGSYLASSLNINAMISLDDIEVASEIKINSINGK
                     DQVKLKANAPKDELRSYKIELEAIVQDPKQWLPENTLPTGIPTLSDLKFKIDFSENED
                     HSNYIYNGNIQINTSEFWPLEAL"
BASE COUNT         2701 a         1711 c         1410 g         2574 t
ORIGIN      
        1 tcaattgtat taagcaagtt tcgcttcctt taaataattt tcgacgtact tcccaatcat
       61 atccacttcg atattgacaa gagtacctgt ctgccaagtg gccaaatttg tttttaataa
      121 agtttcagga ataatagcaa caacgatccc aagctcagtc ttttctgcaa cggttaagct
      181 tatgccgttt aaggtaacag aaccgcgacg aataatataa cgtcgcaaat cacttggtat
      241 ctcaagttcg acttcgattc ccgtttctcg ttccacgcga gagtgcactt tgatcgcact
      301 atctacatgc cccattacaa aatgcccacc cattaaactt tgtggtgtac atggcaattc
      361 caaattcacg agatcgccta ctttagtatc aataaaagat gtgtttttta cggtctgatg
      421 aatcaaacaa aaagtggcct catcttcagt acaattttca atagtcaagc aaacgccatt
      481 atttgcaacg ctatctccca ccttactatc tttaaaaaat tgtgaagcaa aaagacgcat
      541 agataaagca tctccacgcc attcgagaga aagtatcttt ccggttgctt gaataattcc
      601 tgtaaacata cgaggtaaaa ttagaaaaaa aaagaatgtc agagtaaaga atggaaagaa
      661 tctcgcctaa attaagttag tttcttcgct ttaccctatt gttactttta gcaacccttt
      721 tcttgaccct tgaagtgaaa cgattatagg ggctttcttt tttagaaaat tatttttaaa
      781 caaaaaaaat agggcctttt ttgaaaaaag ccctattgat aatactagat aaaagaatta
      841 catgtcttcg ccgtaaactt tttctgcata aagagctgtg gcacctaggt attcgcgatt
      901 catttttgca atgaaatcta cggtgatact cttagggcat gctgcttgac attcgtagag
      961 attagagcag ttaccaaagc cttctttatc catttgtgcc accatggcaa gtacacgctt
     1021 tttagcttct actttacctt gaggcaaata gcttaagtgg cttacttttg cagctacaaa
     1081 aagcatagcg gaagagttct tacaagcggc aacgcaagca ccgcaaccaa cacaagcggc
     1141 agcatcaaaa gcacgatcag cttggtcttt tggtacagca atggtcgctg catctggagc
     1201 agagcctgta ttcacgctca caaaaccacc agcttggata atgcgatcaa atgcagagcg
     1261 atctacagca cagtcccgaa tgacaggaaa tgctgaagca cgccaaggtt caattacaat
     1321 ggtatcgcca tctttaaatt tgcgcatatg taactgacat gtggtgatcg catggtcagg
     1381 cccatgaggc ataccattaa tcacgagaga gcacatacca caaatgcctt cacgacagtc
     1441 atgatcgaaa gcaaaacctt ctttaccttc tttcatttgt tgttcattga cagtgtcaag
     1501 catttctagg aaagacatat ctggagaaat gccacctatt ttaactgttt caaactgtcc
     1561 tttcgatttg gaatcttttt gacgccaaat tttcagagtc aaattcatat taccactcat
     1621 tatttataac tccgggtcac aaggtgaacg ttttcaaatg ttaaaggttc tttagataat
     1681 tcaggttttt gattattgcc cttatattcc caagcaccaa catagcagaa tttttcatca
     1741 tcgcgttttg cttcgccttc ttctgtttgg ctttcctcac ggaaatggcc accacaagat
     1801 tcttcacgat gtaaagcatc ttgagccatt aattcaccaa attcaaggaa atcagctaaa
     1861 cggctagcat gctcaagatt ctggttaaag gatccttcac tgccaagaac atttacattc
     1921 ttccagaatt cttcacgtaa ctcaggaatt aaagccaagg ctttctctaa gccagcttta
     1981 ttacgtccca tacctacgtt ttcccacatg atgtggccaa gttcacgatg aatatcagaa
     2041 acagtacgct ttcctttaat atttaaaagc ttatgaatac gttctgaaga ttctttcttt
     2101 gaaacttcaa attctttttc agtagaagaa attttctcaa gttttgtgct tgcaaaataa
     2161 ccaccaatgg tataaggaat cacaaaataa ccatcagcta aaccctgcat aagagcagat
     2221 gctcctaaac ggttagcacc atgatcagag aagtttgctt cgccaagaac aaagcatcca
     2281 ggaatagtac tcattaagtt ataatcaacc catagaccac ccattgtata gtggactgca
     2341 gggaaaatac gcataggtac tttatatgga ttctcatcgg taatcttttc gtacatttgg
     2401 aagaggttac catactttgc actgacagca gaaacaccta aacgcttgat ggcatcagag
     2461 aagtctaagt aaacagctaa tttagattga ccaacaccca ttccgttatc gcaaacgttt
     2521 ttagcattac ggctagctac gtcacgaggc actaaattac caaagcttgg gtatttttct
     2581 tctagataat aaaatctttc tgattcagga atttgatctg gagaacgagt gtctccagcc
     2641 ttttttggaa cccaaacacg gccatcattt cgaagagatt cactcatcaa agttaattta
     2701 gactgatggt ctcctgtgac aggaatacag gttggatgga tttgagtata gcaaggatta
     2761 gcaaatacag cacccttttt atatgcactc caagcagcag aaacgttaga accctttgcg
     2821 ttagtggaga gataaaagac gttaccataa ccaccagagc atagacatac ggcatcagct
     2881 tcatggcttt caagttcacc agtaacgagg ttgcgcacga taataccacg agccttacca
     2941 tcgatcacca ctaagtcttg catatcgcga cgtgggaaca ttttcactgt tcccttagcg
     3001 acttggcgca tcattgcttg ataagcacca attaaaagtt gttgaccagt ctggccacga
     3061 gcataaaatg tacgtgaaac ttgagcacca ccaaaagagc ggttatctaa aagaccaccg
     3121 taatctcttc caaaaggaac accttgagcc acacattggt cgataatcaa atttgagcat
     3181 tcagctaaac gatgaacgtt ggcttcacga gcacggaagt caccaccttt tactgtatca
     3241 tagaataaac gatatacgga gtcgccatcg ttagtataat tttttgccgc attaataccg
     3301 ccttgagcag caatagagtg agcacgacga ggactatctt gaatacagaa agccttaacg
     3361 ttgtagccta attcaccaag tgaagctgca gcagaagcac cagcaagacc tgtaccaacg
     3421 acgatcacag taaacttacg tttgtttgct gggtttacaa gcttcaattc aaatttatga
     3481 cgagtccatt tttcactaat cggaccacct gggattttag aatcgagaat cattaacggc
     3541 ctccgttcat ttcaaaaaca taggaaactt gagtattttc aattgaagaa ggcaaaatga
     3601 aagcaccttt ttcagcctct tccatcttct tttgttcttc aagcattttc tgcttgccaa
     3661 tatctctaga tttttcaata agatcttgtg tttcaggctt gttccctaaa ataaaggaag
     3721 ccacagcgat caaagcaaaa ccaagagcga caacaacact atagactatg ccaagtaatt
     3781 caataaatgg ggtccacttt tggtgtgcta ttcccatagt ttggaaagca gaagaaatcg
     3841 catggaacaa atgaagccca acaataaaca tacttacaac ataaaaagca gagaacttaa
     3901 tatctgcaaa agcaatgatg gtggtgagcc acatatcacg gattactctt ccatcagcaa
     3961 catcaggact ctggtagaaa aagtattcac caaattttaa ggacattaag tgagacacta
     4021 aaaagaaaac aacaaataag ccagaccaga tcatagtgaa agtagcaaaa gtctttttgc
     4081 cttttctagc attgacatca taatcactgc caccacgagc ttttttattt tgaagtttta
     4141 atgttaccgc taaaaatacg tgtaaaataa aaacagccat catcacagct tctacagtat
     4201 agataaaagc tttatgaccc gttagtagtt cacaataagc gttataagag gcctgagcgg
     4261 caagtggatc caagttgatt aactgaatgt taccaaccat gtgtcctaga atgaacatag
     4321 caagaattgc tcctgtgcat cccattattt gttttttgcc aatcgaggat gtgatgtatt
     4381 taattatcca ttccattgat gtatctcctt aaagttcatt caattatttt gccatcacac
     4441 aacaagcttt ttgacttgct gcatcataag cgtaaatgtt ttctttttca aaccaaaaag
     4501 ctatttctct agcggcactt tccacaccat ctgaactgtg aaccacattt tcagtcatgg
     4561 aaggggcaaa atcatgacga atcgttcctg gttcagattt agctggattt gtggctccat
     4621 taatcgctct gacttttgcc acagcatttt caccaccgag cacaagcatc acagaagggc
     4681 ctttagtcat atattcttcg agttcaccaa aaaagggctt ttccacgtgt tcagcgtaaa
     4741 agcccctagc gtcagcagaa gtcatctggt gcatttttat agcacaaaca gataatcttg
     4801 cactctcata acgttcaata attcgcccta ctaagccaga ggcaacgcca ttaggcttaa
     4861 tcatagcaaa agtcatttcc ataaaatacc acctttttgt gtttcgcaaa tataagtaaa
     4921 aataggaacc taaaaatagg tccctaaaag aagattttat ttattcggct tttttgtcag
     4981 aaattgaacc taaggaatcg gtctcctcta ttttagaaat tttttccaca agttcttgag
     5041 ccaattttcc attagattct atgttcttaa ttaaccattc cacagactct ttgagttcac
     5101 ttttaggata tttcgtgatg aaatccttaa ataaaggaag agctaaggaa tcatttttta
     5161 agttttcaga caaaacaaaa gctctactaa ataacgcttt ttctgaatta ggatctgttg
     5221 gccagagact ataatagact ctgtattcag actctgcatt gttgtaactt tcattttcat
     5281 tgtcgatcgt tgctatttca aaagttgctt tcttgtaggc tgcttcgtta tctggataaa
     5341 gagaacgaat taattcataa ttagcacgag cttcgtttag cttacgatta ttatagaatt
     5401 gttctgcttc taagaagagt gaatcagaag aaaaaattgt ttttaaagca ggcatcttag
     5461 aattaaagac ggttacctta tacttactcc acatatctct ttctaaacgt ttccattgat
     5521 tactttcttc aattgaagaa atatttctaa agaccattcc acgagcagct tcaaaatcag
     5581 caaaagaacg ataagcatcc atattgaaat aatattctct cttaagaaca atttctggtg
     5641 tttttaaata aattttcagt tgtggaataa gttcttcaaa ggtggccgta ggagcaatct
     5701 tcgcaccaac ttgatcaaaa acggttttaa gatcttcatc tgaaaattct tttttgagtc
     5761 ttaaagaatc tttaaaatat tgattcgtga gatcacggcg agtttgacgc acaaaagcct
     5821 tgtattccca tgattgatcg agatcaaaag acttcgcttc cattgcatat acagcccatt
     5881 gagttaaaaa gtctatcaaa cgagtgcgat taaaagcaac acgttgactt tctggaattt
     5941 cattacgaag agcaattaaa tcactttctc tcactaaagg cttaccttga gcagtgacta
     6001 aaacaaacga ggaatccagc tttagattat catcgttcgc tatttcattt aaaaccgtag
     6061 aacgtactcg atcaaaggat tttggctctg ctgggatttc tttttcaaga taaaatacat
     6121 ggtatttttg tgtttttggt gcttttataa tagaagaaat agttcctgct ggtttacccg
     6181 taaactcatc aaaaacttga ggaacaagcc ctataccata aggcatgcta tgactttttt
     6241 taacactccc tactagacct tcttgttctt ttgtaaaagt attttgagat aattctgttg
     6301 cttttttctt aaaatcatca agagaagaaa cctgatctaa aacatctttc tttaatatag
     6361 ctgaatcgct atgctctata tgataaaggc tatagccagg taatgtctta tataattcta
     6421 tatgagaatc ataatatttc ttagcttcag cgtcagatat aggctgtttt acaacgtcat
     6481 attttttctc aaaacgtcga taagattcac gcatcatatc tcgagtatag ttatcaataa
     6541 acatacgatg aagatcagcc aaatgttctt ctagaacagc ggccttcgtt tcttttctgg
     6601 aaatcatttg tacagtataa aaggtggaat catttaagga atccacaacg gcaatggtct
     6661 taggaactga atccacagga agagctgaat cgccaaaaat aaatggtttt aattctttta
     6721 gagctgttaa ttcatgatat attttttcat ttacaactgt acgattaact cctttgattg
     6781 aatctattgg agtctttgca agaatagcgg attcaatttt tgatgattct ttcttggtat
     6841 ccgatttaaa gaaataaagt tctgctagag ctggttcact aaaattagat aaattctgtt
     6901 caataaaacg atttacggaa tcagcattgg cctctataaa aagcttttga gccacacttt
     6961 ttcgaatatc attaaatgtt tgagttgaat ccatggggaa ggaatcttta ttagcctcat
     7021 aatacttttc aacttcactt tgcttaaatc caaacatttc aagaacatga tactgctgat
     7081 aaaccatagt cattagacgg ttatccatgc gtttgagatc atcgtccact tttgaagcta
     7141 ttccaggata cgtggtacga gcaacagcag ccataccctc gtttaaaata acttgattaa
     7201 aaatgtcggc aaaacgtgcg gatttttttt ctgcattagc aagtttttgt gataattcca
     7261 catcggaaag atataccttt tcactcccta tacgagcgac taactcttct ttagtcccca
     7321 aagaattaca cccaactagg cttagtaaag taaaagaaag taaaagaatt tttttcgaat
     7381 tcatttgtat cctcatgtag aagaactatt taaaataact tgtccaaaaa atataaaaat
     7441 cagcttttta gacaattgaa ttgaaacgaa atatacatat tctaactata ttatggaggt
     7501 tgtctaaaga aacagatcct caacatcagt cttctaaaaa taagaattgg tcaaggagac
     7561 tgattgctgt cgcatttttt gcgatttttg taagcagtgt tgtcttcacc gcttggactc
     7621 ttttttattg gctggatcat ttttttaagg aaaaacagac ttatggttcc gtagtagtag
     7681 aaccatttca atattcaaga gatcatattt tatcacatca ctgggattct atccttgttt
     7741 atagccccga tttttcaatt tcaatccaaa agcctcattt tcgaattttt gatcttcttt
     7801 caagcaaacg atcggcctta ttaaaagtag agcatgtaaa atgccacttt tctactcaac
     7861 ccaaagactc tacctcagcc cccttttcac tcgactccgt aaaaattcca aatattaaaa
     7921 tacctatacc cgttttcatt gaattagact ctagtttggt agaagtcgat tctactctct
     7981 tttggaaaac tcaagggcta aaaattaaca atgaatcagc gaacaacgtc atattatcta
     8041 tagacaccat agacggaagt tatctagctt cttctttgaa tatcaatgca atgatatcgc
     8101 tagacgatat agaagtggca tctgaaataa aaataaattc aatcaatgga aaagatcagg
     8161 tcaaactcaa agccaacgct cccaaagatg aacttcgatc ttataagata gaactagaag
     8221 ccatcgttca agatccaaag caatggttac cagaaaatac tctaccaaca gggataccca
     8281 cgctttctga tttaaaattc aagatcgatt tttcagagaa tgaagaccac tccaattata
     8341 tctataatgg aaacattcaa ataaatacat ccgaattttg gcctctagaa gcttta
//
LOCUS       DAOOBB010000077         8306 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_10462_length_8306_cov_5.173434, whole genome shotgun sequence.
ACCESSION   DAOOBB010000077 DAOOBB010000000
VERSION     DAOOBB010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 8306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8306
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10462_length_8306_cov_5.173434"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..1602)
                     /locus_tag="PLT31_04880"
     CDS             complement(64..1602)
                     /locus_tag="PLT31_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HPW94508.1"
                     /translation="MSTKYSKRGILISKTPYEKRIAIMEDGELAELVVEGLSSNRVLG
                     NIYKGVVQKVLPALKAAFIDIGLEKAGFLHQDDAIDRNAQLRREFGDGDDEEGNEDPI
                     SIDEILEEGQEIMVQVVKEPISTKGARLTTHLSFAGRFLVCMPGTNFIGVSKKEKDPY
                     RRREFKKVVRRLKGRDVGYIVRTNGLGESELEISKQMRELEAKWEATKYNFSVLPAES
                     CIYEESDSIEQTIREYFSDNTDYVYIDNREEFLAITEYLKMLSPDKLNKVKLWSSSES
                     LFEYFKIEDDYARSLQRQVPLPRGGNLVIEQTEALVSIDINTGPKVHGKDQALIIRET
                     NIDACYEIAKQLRLRDVGGLIIIDFIDMDNDADKETIYNEFRKAIRRDKAPISAAPIS
                     QFGLMEVTRKRVRANLMTEKTEVCPVCKGSGHIYSLETSLSIIDRSLSRARTKGRLKE
                     VTLVVSTPMINVFCNDFGRMFNYLEYKHHMKINLVEDERSYVNQFSIYDSKGEEITDL
                     FESI"
     gene            complement(1729..2679)
                     /locus_tag="PLT31_04885"
     CDS             complement(1729..2679)
                     /locus_tag="PLT31_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94509.1"
                     /translation="MKNAFEQNIIAMVWDFDKTLICNYMQEPLFRRYNIQANAFWNEI
                     NRLQQFYAKRNININRDTCYLNHILTYVKSGLLKGLSNRILRECGKELEFFPGLPDFF
                     SDIKNQVEEDSLFKKYGIKLEHYVVSTGFAETIRGSAIAPYVDGIFGCEFIESPARPG
                     YLDEDPIEPESEISQIAIALDNTSKTRYLFEINKGSNKFSQEIDVNSFISKENRRIPF
                     ENMIYIADGPSDVPAFSILNSFGGSTFAIYPKGDKKAFQQVEQLRKDKRIQTFGEADY
                     TNGSHSWMWLSEQVNSIAQKLVLQKEESIRKSSSAPPGHL"
     gene            complement(2683..3501)
                     /locus_tag="PLT31_04890"
     CDS             complement(2683..3501)
                     /locus_tag="PLT31_04890"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPW94510.1"
                     /translation="MKKIDIHFFPSNTEKPRSFRISLLRPIWWVIGALVAALGFILFS
                     PIQIFDTITNGHLINLYRQNRQIEQTIADIHQSIQESEKQLQEASSLRDSSIKLSGLI
                     SPVADIEKDSNDRPSISQVFTTYKKFLDSLEKYPEQGPAIPISLPVKIKPIITNRFSF
                     ILDPFTQQELPHRGVDFAAVEGDTVYATGAGKVIDVRHHRGFGLTVKIEHTSEIRTFY
                     AHLNNSLVRQGSTVKRGTPIAIVGKSGRSTGPSLHYEIRLQGQPINPEPLLIMM"
     gene            complement(3488..3772)
                     /locus_tag="PLT31_04895"
     CDS             complement(3488..3772)
                     /locus_tag="PLT31_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94511.1"
                     /translation="MSETPLNKLKNGALNCASALLLRVDLAAEESRLNDKFKILGKKV
                     QEFIKNDSLDTLKDDPSIVELIGAIHENEVRIQELKKRCSKDCSGSSEKD"
     gene            3838..3987
                     /locus_tag="PLT31_04900"
     CDS             3838..3987
                     /locus_tag="PLT31_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94512.1"
                     /translation="MSRTERRRGKKNAKNFVPKKSQQMDKRIIIALAVIAVVFFVGTT
                     LIKYS"
     gene            4016..5137
                     /gene="dnaN"
                     /locus_tag="PLT31_04905"
     CDS             4016..5137
                     /gene="dnaN"
                     /locus_tag="PLT31_04905"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPW94513.1"
                     /translation="MKFQVEKAVLQDVLQSAISAVPNKSTLQILNNFSLRLEGNFLEV
                     SATDLDLGIRVKVEVQGERDGAVVVNARKLFDQIKNLVDPSITTIDFDVQDYLTTIRW
                     SERGKASITGFDASDFPPFPEIEDGEHIVLAAGELAFLADKTLFATSNDTTRLTLNGV
                     YLESKDGFVSMVATDGHRLGKALLEQEGVVLERGIIVPPKAIQHVLRAVSSDASIEIS
                     ISSSYIMFSSDGVQVISKLIEGPYPKYENVIPQKFERTVQAKTVEFLNKIRSVISMAN
                     ARTRQIRLQIDGNALDLSAADPNVGGDSYEALAVSHEGEGSFSIGFNGQYLSEILSMC
                     KSEEVLLKMNSPVGACIIEPVGEGMNFSFLLMPLRLVDE"
     gene            5226..5945
                     /locus_tag="PLT31_04910"
     CDS             5226..5945
                     /locus_tag="PLT31_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis TtcA family
                     protein"
                     /protein_id="HPW94514.1"
                     /translation="MSAYIKKRISKKITKAMHDFDLIKEGDKVLVAVSGGKDSTTMLQ
                     ELASRLGKADPNYQLEAIYIQADFADSSPKDFLKPFCEKLSIPFHFLNVAIEKRLKEN
                     RDLNCYWCSTQRRTELIQFAIKNDFNKIALGHHLDDIIETLLMNMVYKGEFSGMPPKV
                     IYDKYPVSIIRPLCYCEENEIKEYSIESDFAQFTCTCPFAKNSHRKRIRKEIESLTQS
                     KSSLKQNIFESMKNIKMDYLL"
     gene            6008..7546
                     /locus_tag="PLT31_04915"
     CDS             6008..7546
                     /locus_tag="PLT31_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HPW94515.1"
                     /translation="MERTPSFIAIDLKSFFASVECVERKLDPLTTNLVVADSSRTEKT
                     ICLAVSPTLKKYGIPGRARLFEVVQKVKEVNQERLRHTKNRVFTGSSFNAIELENNPN
                     LKLDYIVAPPEMARYMEVSTQIYNIYLKYIASTDIHVYSIDEVFMDVTAYLKTYQMSA
                     HDLAMKIIQDVLKTTGITATAGIGTNLYLAKIAMDIEAKRIPADENGVRIAELNELTY
                     REKLWDHRPLTDFWRVGRGYQKKLEANGLMTMGDIAACSIGRFRRDSSKYNEELLYKL
                     FGINAELLIDHAWGYEPCTIAQIKAYKPSNNSISLGQVLTSPYEFSKAKLVILEMTDS
                     LVLDLVRQNLVTDQIVLTIGYDVENISDPKRNYLYHGEVVLDHYGRKIPKPAHGSINL
                     KNYTSSTKMIMEAVSELLDKIADKSLLVRRMYVVANHIILESKMNAIKGANKQFDLFN
                     DTQEKQKEEMAQKELNEKEKKIQKAVLSIKNKFGKNAVLKGMNFEEGATAKTRNKQIG
                     GHKA"
     gene            7543..7971
                     /locus_tag="PLT31_04920"
     CDS             7543..7971
                     /locus_tag="PLT31_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94516.1"
                     /translation="MNPKYKDLLHLPHPSSLKHPRMDPIDRAAQFSPFSPLTGYDEAI
                     SETARETNQRIELDEEARNKLDAQLATILLRLKEKPPVTITYFKADKTKEGGSYETIT
                     GVIKRINSTKNAIQMENGPEIIIQDIISIRHELDNTYTNT"
BASE COUNT         2770 a         1512 c         1533 g         2491 t
ORIGIN      
        1 tttaaaataa aaaagccgat aaagagtatt ctttttatcg gctttgaaat agagtatcaa
       61 aaattaaatc gattcaaata aatcggtgat ttcttcgccc ttagaatcat aaattgaaaa
      121 ctgatttacg taggaacgtt catcttctac tagattaatt ttcatatgat gcttgtattc
      181 taaataatta aacattctac caaaatcatt acagaaaacg ttaatcatag gagtacttac
      241 aactaaggta acttctttta agcgaccttt tgtacgtgct ctagatagag aacgatcaat
      301 aatagaaagt gatgtttcta aactgtagat atgaccgctt cccttacaga cagggcaaac
      361 ttctgttttt tctgtcatca agttagcgcg cactcttttt ctagtgactt ccatcaagcc
      421 aaattgacta ataggagctg cactgattgg agctttatct cggcgaatgg ctttacggaa
      481 ttcattgtaa atggtttcct tatcggcatc attatccata tcaataaagt cgataataat
      541 taaaccacca acatcacgta atctaagttg tttagcaatt tcatagcaag catcaatatt
      601 ggtttcacga ataattaatg cttgatcttt gccatgaact tttggccctg tattaatatc
      661 aatagaaaca agcgcttctg tttgttcaat caccaagttt ccaccacgag gaagaggtac
      721 ttgacgctgt aaagatctcg cgtaatcatc ttcgattttg aaatactcaa ataaactttc
      781 tgaagaactc caaagtttta ctttgttgag tttatcagga gaaagcattt ttaaatattc
      841 agtgatcgca agaaattctt cacgattatc aatataaacg taatctgtat tatcgctgaa
      901 atattcgcga atggtttgtt caatagaatc cgattcttcg taaatgcaag actctgcagg
      961 aagaactgaa aaattgtatt tagtggcttc ccatttcgct tctagctcac gcatttgctt
     1021 tgaaatttca agttcagatt cacctaaacc gttggtccga acaatataac cgacgtctct
     1081 tccttttaag cgacgaacga ctttcttaaa ttcgcggcgg cgataaggat ctttttcttt
     1141 tttagaaacg ccaataaaat tagtgcctgg catacatact aaaaaacggc cagcaaagct
     1201 taagtgagtt gttaaacgag cccctttagt actaataggc tctttaacca cctgcaccat
     1261 aatttcctga ccttcttcta atatttcgtc aatggaaata ggatcttcgt taccttcttc
     1321 gtcatccccg tcaccaaatt cacgacgaag ttgagcgttg cggtcaatcg catcatcttg
     1381 atgtaagaac ccagcttttt caagaccgat atcaatgaaa gctgctttta atgcgggtaa
     1441 aactttttga acaacacctt tataaatatt acctaaaact cgatttgaag aaagaccttc
     1501 tacaacaagc tcagccagtt cgccatcttc cataatggca atacgttttt cataaggtgt
     1561 cttactaatc aaaataccac gtttcgaata cttagtagac ataaaactcc tagggagaaa
     1621 aaaatctttg gataaaatta tccaatttta gatatgtaaa tataagaata aagggtctac
     1681 tctctaaaag agaaatttct ttttaggggg cagaggctta tttttttctt ataaatgccc
     1741 tggaggcgct gacgagcttt tccgaatact ctcttctttt tgcaaaacca gtttttgggc
     1801 gatggaattc acttgttcag aaagccacat ccaggaatgg gaaccatttg tgtaatcggc
     1861 ttcaccaaag gtttgaatgc gtttatcctt tcgcagttgc tccacttgct gaaacgcctt
     1921 tttatcgcct ttagggtata tggcaaaagt agaaccgcca aaactattta aaatactgaa
     1981 agctggaaca tcagaaggac catcggcaat gtaaatcata ttttcaaaag gaattcgacg
     2041 attctcttta gaaataaaag agtttacatc aatctcttga gaaaatttat tactgccttt
     2101 attaatttca aaaaggtacc ttgtcttaga ggtattatca agagcaattg cgatttgaga
     2161 aatctcactt tctggttcaa tagggtcttc atccaaataa ccaggacgag caggagattc
     2221 tataaattca caaccgaaaa taccatctac ataaggagcg atggcactac cacgtatggt
     2281 ttctgcaaag ccagtactta caacgtaatg ttcaagttta atgccatatt ttttgaacaa
     2341 actatcttct tctacttgat ttttaatatc agaaaaaaag tctggtaaac caggaaaaaa
     2401 ttcaagctct ttgccacatt ctctaagaat acggttagac aaccctttta agaggcctga
     2461 ttttacataa gtcaaaatat ggtttaagta gcacgtatca cggttgatat taatgtttct
     2521 tttagcatag aattgctgga gacgattaat ttcattccaa aaagcattgg cttgaatatt
     2581 gtatctgcga aacaaaggct cttgcatgta attacatata agcgtcttgt cgaaatccca
     2641 aaccatagca atgatatttt gttcaaaagc atttttcatt ttttacatca tgatcaataa
     2701 aggttctgga ttgataggtt gaccttgcaa cctaatttca taatgtaggc taggtccagt
     2761 cgatctacct gatttcccta caatcgcaat aggagttcct cttttgacgg tagaaccttg
     2821 acgcactaga ctgttattta gatgagcgta aaacgtacga atttcagaag tatgttcgat
     2881 ttttactgta agcccaaaac cacgatgatg acgaacgtca attacttttc cagcccctgt
     2941 cgcatagacg gtatcgcctt cgacagcagc aaaatcaacg cctcgatgag gaagttcttg
     3001 ttgagtgaaa ggatcaagaa taaagctaaa acgattcgta ataataggct ttatttttac
     3061 tggaagacta ataggaatag caggcccttg ttcagggtat ttctcaaggg aatcaagaaa
     3121 cttcttgtat gtagtgaaaa cttgagaaat gctaggacgg tcgttggaat ctttttcaat
     3181 atcggcgacg ggagagatta gtccactgag tttaatagaa gaatctctaa gtgaagaggc
     3241 ttcttgcagc tgcttttctg actcttgaat ggattgatga atatctgcaa tggtttgctc
     3301 aatttggcga ttctgtctgt acaaattaat taagtgacca ttggtgatgg tatcaaaaat
     3361 ttgaatagga gaaaaaagaa taaaacctaa ggctgcaaca agagcaccaa tgacccacca
     3421 tataggacgg agaagagaaa tacgaaaaga tctcggtttt tctgtattcg atgggaagaa
     3481 atggatatca atctttttca ctggaaccgg aacaatcttt agaacaacgt ttttttagtt
     3541 cttgaattct aacttcattt tcgtgaatgg caccaatgag ttctacgatg cttggatcat
     3601 ccttaagagt atctaaagaa tcatttttaa taaattcttg aactttttta ccaaggatct
     3661 taaatttgtc attcaatcta gattcttctg cagcgagatc tacacgaaga agtagagcag
     3721 atgcacagtt aagagcacca ttttttagct tatttagagg tgtttctgac attgtatttc
     3781 tccttaatcc tttctaaaat agtagttttt atgctataaa acaatggaga tctttttatg
     3841 agtagaactg aacgcagacg tggcaaaaaa aacgccaaga attttgttcc taaaaagagt
     3901 cagcaaatgg ataaacgtat cattattgcg cttgcagtga ttgccgtcgt cttttttgtt
     3961 ggaacaacgt tgataaagta ttcttaaggt attctgttta aaaggaaaat gaacaatgaa
     4021 gtttcaagta gaaaaagcgg ttcttcagga tgttttgcag tccgcgatta gtgcggtgcc
     4081 aaataaatcc actctgcaga ttctaaacaa tttctctttg cgactagaag ggaatttttt
     4141 ggaagtaagt gctacagatc ttgatctagg tattcgagtt aaagtagaag ttcaagggga
     4201 acgagatgga gctgttgtag taaatgctcg taagctattt gaccaaatca aaaacttagt
     4261 ggacccaagt attactacca tcgattttga cgtacaagat tacttaacca ccattcgctg
     4321 gagtgaacgc ggtaaagcaa gtatcactgg ttttgatgca agtgatttcc ctccattccc
     4381 agaaattgaa gatggagaac acatcgtttt agctgctggc gagcttgctt tccttgcaga
     4441 caaaactttg tttgctacct ctaatgatac cacgcgttta actttgaatg gtgtttatct
     4501 agaatcaaaa gacggctttg tatctatggt tgccaccgat ggtcatcgtt taggtaaggc
     4561 gttattagaa caagaaggcg tcgttttaga acgagggatc attgttcctc ctaaggcaat
     4621 tcaacatgtg cttagagctg tctccagtga cgcttctatc gagattagca tttcctcttc
     4681 ttacatcatg ttctcaagtg atggcgtaca agttatttcg aaattaatcg aaggccctta
     4741 tccaaaatat gaaaacgtga ttccacaaaa gtttgaacgt accgtccaag ccaaaacggt
     4801 ggaattctta aataagatta gaagtgtgat ttctatggct aatgctcgta cacgtcagat
     4861 ccgtttgcag atcgatggga acgcattaga tttaagcgct gcagatccaa atgtcggcgg
     4921 tgattcttac gaagccttag ctgttagtca tgaaggagaa ggttcattta gtatcggctt
     4981 taatggccag tatctttctg aaattctttc catgtgcaag agcgaagaag tacttctaaa
     5041 gatgaatagc cctgttggtg cttgcattat tgaaccagta ggcgaaggga tgaacttctc
     5101 tttcttactc atgcctttac gtcttgtaga cgaatgagtc tttgaaaaga gaagccttaa
     5161 aggctattaa atagggctct ttcgggagct ctttttttta gagtgccgta tagaggatga
     5221 tgtcgatgtc ggcttatata aaaaaacgaa tcagtaaaaa aatcacaaaa gccatgcatg
     5281 actttgatct tatcaaagaa ggcgataaag tattggtggc tgttagtgga ggaaaagact
     5341 ctacaaccat gcttcaagag cttgcttctc gtttaggaaa agcagacccg aactatcaat
     5401 tagaagcgat ctatattcaa gctgattttg cagactcatc gccaaaagat tttttaaaac
     5461 cattttgtga aaaactttcg attccatttc attttttaaa tgtcgctata gaaaaacgac
     5521 taaaagaaaa tcgagacctc aattgctatt ggtgttcaac gcaacgtaga acagagttga
     5581 ttcaatttgc aataaaaaat gattttaaca aaatagctct cggccatcat ttagacgaca
     5641 tcattgaaac gttattgatg aatatggttt ataagggaga gttttctgga atgcctccaa
     5701 aagtcattta tgataaatac cctgtatcta ttataaggcc gctatgctat tgtgaagaaa
     5761 atgaaatcaa agagtattca attgaaagtg actttgcaca atttacttgc acttgcccat
     5821 ttgcaaagaa tagccatcga aagcgaattc gaaaagaaat agaatcatta acgcaaagca
     5881 agagctcctt aaaacaaaat atttttgaaa gtatgaaaaa cattaaaatg gattatttgt
     5941 tgtaaatttt tttcatctca aaaaaacaat gtcacatttt gtctaaacat ttatgcattt
     6001 ttaatacatg gaaaggactc catcttttat tgcgatcgat ttgaaatcat tctttgcctc
     6061 cgtagaatgt gtcgaaagaa aattggaccc tctaaccaca aatttagtcg ttgccgattc
     6121 tagtcgaaca gaaaaaacaa tttgcttagc tgtatctcct accttaaaaa aatatggtat
     6181 tccaggacga gccaggctct ttgaagtagt gcaaaaagtg aaagaggtaa atcaagaacg
     6241 cttgcgacat acaaagaatc gtgttttcac agggagctct tttaacgcga tcgaattaga
     6301 aaataatcca aatcttaagt tagactatat tgtagcgcca ccagaaatgg ctcgttatat
     6361 ggaagtaagt actcaaatat ataacattta cttaaaatat attgctagta cagatattca
     6421 tgtttactct atagatgaag tctttatgga tgtcacagcc tacttaaaaa cgtatcaaat
     6481 gtcggctcat gatttagcaa tgaaaatcat tcaagatgtt ttaaaaacaa cgggaatcac
     6541 agcgacagct ggaatcggga caaatcttta cttagcaaaa atcgcgatgg atatagaagc
     6601 gaagcgtata cctgcagatg aaaatggcgt acgtatagca gaattaaatg agctaacgta
     6661 tcgagaaaaa ttatgggatc atcgtccgct tactgatttt tggcgcgttg gaagagggta
     6721 tcaaaaaaaa ttagaagcaa atggcctcat gaccatgggc gacatagccg cttgttctat
     6781 tgggcgattt cgtcgagatt cttctaaata taatgaagaa cttttatata aactctttgg
     6841 gataaatgca gaacttttaa ttgaccacgc ttggggttat gaaccttgta ccatagctca
     6901 aatcaaagcg tataagccat cgaataatag cataagcctt ggccaagtac tcacttcgcc
     6961 ttatgagttt tctaaagcga aactcgtcat tttagaaatg acagactcgc tcgttttaga
     7021 tttagttcgc caaaatttag tcacagatca aatcgtttta acgataggtt acgatgtaga
     7081 aaacatatca gaccctaaac gaaattactt gtatcacgga gaagtcgttt tagaccatta
     7141 tggccgtaaa attccaaagc ccgcccacgg ttctattaat ctcaaaaatt atacttcgtc
     7201 cactaaaatg atcatggaag ccgtgtctga attattagat aaaattgcag acaaaagcct
     7261 tttagtccgc cgtatgtacg tagttgcaaa ccacattatt ctagaatcaa aaatgaatgc
     7321 cataaaagga gcgaataaac aattcgattt gtttaatgat actcaagaaa aacaaaaaga
     7381 agaaatggct caaaaagagt taaacgaaaa agaaaagaaa atacaaaaag ccgttcttag
     7441 cataaaaaat aaattcggaa aaaacgccgt tttaaaagga atgaattttg aagaaggggc
     7501 tacagccaaa acgagaaata agcaaatcgg agggcacaag gcatgaaccc aaaatacaaa
     7561 gatttgcttc acttaccgca cccgagttct ctaaaacacc cgagaatgga tccaatagac
     7621 agagccgcac aattttctcc tttttcgcca ctcacaggat atgatgaagc catcagtgaa
     7681 acagcaagag aaacaaatca aagaatcgaa ttagatgaag aagcaagaaa caaattagat
     7741 gctcaactag ccacaatttt attacggtta aaagaaaagc ctcccgtaac aatcacctat
     7801 tttaaggccg ataaaacaaa agaaggcggc tcttacgaaa caatcacagg cgtgatcaaa
     7861 agaatcaata gcaccaaaaa tgcaatccaa atggaaaatg gcccagaaat catcatccaa
     7921 gatataatct cgatccgcca cgaattagat aatacataca caaatacttg aataataata
     7981 gtttgtctct ttttgttcct attttcactt ggtcgccatc aaatttttat caccaaagca
     8041 attgtttaag gaaacctata ttgcacattt aaaaagctct ttcacaccgc aagagttttt
     8101 ttgttaaaaa aagtgatggg ggttgagggc tgacgattat gaggctttta agtttcgcga
     8161 gaagcgtcgc ggagaatttc gcgaaagacg tctcggggga tatcgcgaga agcatctcgg
     8221 ggaattcaca atatcatatc gatactccgt ttttttcaca aaaaacattc aaaaatgatt
     8281 tttcaatatt tatattaaaa agcaaa
//
LOCUS       DAOOBB010000078         8287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_10486_length_8287_cov_5.013848, whole genome shotgun sequence.
ACCESSION   DAOOBB010000078 DAOOBB010000000
VERSION     DAOOBB010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 8287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8287
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10486_length_8287_cov_5.013848"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="PLT31_04925"
     CDS             <1..1096
                     /locus_tag="PLT31_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94517.1"
                     /translation="GLRKLEYPIKPHLILFDASTTTGIDLQNTFETLIKKYNLYETDS
                     GRKNGFKIIGWSTVWDNDEQQVDKKSGNKKNRLKDLFPDYSKESNAKKEHFDCLKKHL
                     HLYDHEKKTFEAVRKSILNALVQVLRLEGILKAPNIPYRKSSLIEFIKDLGTETYNDF
                     IAKLFKWCFDLVVKKNFEKVYIEIKQFIENDFIKWNWLNETDVTARSIKHAKDFIESN
                     NYTFQSHLTKEIINEGKEINPYNIEINSVHAVKGQTHCATMYVETSYHEYETKKLKVI
                     SKEATKTKPEELLPYPLFFNEQSYRQNKDVRAKEAIKMMYVGFSRPTHLLCFAALKDN
                     IGDIKPYKDAGWEVISDLILSSVENGENDE"
     gene            1093..2121
                     /locus_tag="PLT31_04930"
     CDS             1093..2121
                     /locus_tag="PLT31_04930"
                     /inference="COORDINATES: protein motif:HMM:NF024706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HPW94518.1"
                     /translation="MSNSPKSQLTIKDELTDFLLYTAPNGKVKVEVFLHNETVWLTQD
                     QMATLFGKAKSTINEHIKNIYDEQELEESATMKKFGISEFQQKTPNYYNLDVIISVGY
                     RVKSPQGTQFRIWATQRLKEYIIKGFALNDDRFKSGNSMNYFDELQDRLREIRLSERF
                     FYQKIKDIYTTSIDYDPKDEKTVEFFKIVQNKLLWAISKQTAAELVYRRVDASLPLLG
                     MQSYDKKGLISVKKSEVSIAKNYLNEDEIKLLGLLVEQYLAFAETMAQQRVPMYMKDW
                     IQRLDIILQLNGRELLTHAGKISHDVAIEKAGIEYEKFRDAQKSIEKEQSLKEIEEDI
                     KNWGKNRK"
     gene            2118..2402
                     /locus_tag="PLT31_04935"
     CDS             2118..2402
                     /locus_tag="PLT31_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94519.1"
                     /translation="MTNSQNNKLTIKDELTDFLLYTAPNGKVKVEVFLHNETVWLTQK
                     AIAILFGVQVPAINKHLENIYESGELNRETTISILETVQIEARNTGSINV"
     gene            2523..2666
                     /locus_tag="PLT31_04940"
     CDS             2523..2666
                     /locus_tag="PLT31_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94520.1"
                     /translation="MEKTYIANRLEMVLGVPAKFWINLEAIYREKLLLIEQENQLSAD
                     IRQ"
     gene            2921..3784
                     /locus_tag="PLT31_04945"
     CDS             2921..3784
                     /locus_tag="PLT31_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94521.1"
                     /translation="MNLRKSEREGPGSRRPHFAHKALSPNCTPEGLLHHSFKMMFADI
                     LKKCIENGWPFPFKKKCDYCGKEHCWDLLKRARDVKIEMDLGSCRPDIMLLNGNGNPY
                     LAIEVVVSHSPEEEVLEYYRKNKIVLFQINLSTEADLNKICERASKPDVFTFCTNPRC
                     SKCGFFMDKRLLAVWTSECYRCRSPMNIAITMTEKNLISGELPSEKTPKYFLEKEIQM
                     AKANGVFIRKHYSKTMDCSYNACTCQRCGAFLGEHYMIDEIMNVVYAYQGGNKDVLKL
                     IDIEYFCPRCE"
     gene            3986..4417
                     /locus_tag="PLT31_04950"
     CDS             3986..4417
                     /locus_tag="PLT31_04950"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPW94522.1"
                     /translation="MDLIFFPDTHPFDPDKSEAKDEGGDVGKEIKGLSKYRQDLYLLV
                     KSFLKALKKVTDITPYLQNKQIYRFTQKYDGLYEMRIPKQKKSGVFRIYFCFSKTNRE
                     KIILLCAELKQKTKPMKLDNALIKLKQYREDENKEVHNEEH"
     gene            4404..4976
                     /locus_tag="PLT31_04955"
     CDS             4404..4976
                     /locus_tag="PLT31_04955"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPW94523.1"
                     /translation="MKNINVLDYMDSLEKEDKDLDLFVKMYEALSSFLTDFDFLKDSM
                     GLTQKDVADKMGTTQSAISRIASMKTNPSYKQLQKMAEAVGGELLITPMKDMTAQVPY
                     DLQNKVRELAKGENKTTNEYLDEVIRDSIQSEFKWYARQTIEVNKVCECPTANGIGLR
                     DFWSNSSKSSLNFGEIKKKTIRFESQAMAV"
     gene            4990..5421
                     /locus_tag="PLT31_04960"
     CDS             4990..5421
                     /locus_tag="PLT31_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94524.1"
                     /translation="MKDNFQQLIKLLQLGDIRFMDSHESVLHIPENILPDSNTSLESG
                     QAFDKESPIIKGDCLLFRVKYVFSFLNNKEPYFKAEYIVNIFFEASDIKKAEELLQDE
                     ELKKIFLEKQLTHTMWPILRGTLMNAFSRHSLNPCMLPWIV"
     gene            5539..>8287
                     /locus_tag="PLT31_04965"
     CDS             5539..>8287
                     /locus_tag="PLT31_04965"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HPW94525.1"
                     /translation="MKFTEEKLEQAFTELLEQQGYTHHLGESIVRNIEDVLIEADLES
                     FLMAKYAHEGITLNEIKSIILQLKSLPASDLYESNKTFLRMLSDGFILKREDRSKKDI
                     YIQFIDYAGLDPQRQPDPEHLIKVASENKKEYDQANQTDKNIYKFVNQLEILGLEKRI
                     PDGIIYINGLPLVVLEFKSAIREDATIFDAYKQITVRYRRDIPELFKYNTFCILSDGV
                     NSKAGSFFAQYDFFYAWRRVAGLAKDVDGIDSMYTLIQGMFHKNRLRDIIRNFIFIPD
                     NSKKEEKIVCRYPQYYAARALYDNIIKAQKPIGDGKGGTYFGATGCGKSYTMLYLTRL
                     LMKSEYFESPTIVLITDRTDLDDQLSAQFLNAKTFIGDNTVQSVESRAHLRELLQGRQ
                     SGGVFLTTIHKFTEDTYLLTERSNVICISDEAHRSQVNLDQKVKVTENGVTKSFGFAK
                     YLHDSLPNATYVGFTGTPIDATLDVFGKVVDAYTMTESVKDEITVRIVYEGRAARVAL
                     NNGELEKIEKYYEEAAEAGANEYQIEQSKKETVNMYAILGDPDRLKAVAEDFVNHYES
                     RIAEGATIKGKAMFVSSSREIAYELYKNIIALRPQWNEIREAEEGAVLTDKDKRELKP
                     IERIKLIATRGQDDPKEMYDLLGTKDQRKELDRQFKNEKSNFKIAIVVDMWLTGFDVP
                     FLDSIYIDKPIQQHNLIQTISRVNRKFEGKNKGLVVDYIGIKKQMNLALAKYNKGDEH
                     NFEDIAESLIIVRNHLDLLAKLFHTFDNSKYFNGTTIQQLNTLNFAAEFVQKTKELEN
                     RFMGLVKRLKAAYDICAGSEKLTQIERNYTHFYLAVRSIVFKLTKGNAPDTSQMNAKV
                     REMIKDALQSDGVEEIFKLGEEAETEQDIFDEDYLAKINKIKLPNTKIKLLQQLLAKV
                     IG"
BASE COUNT         3045 a         1316 c         1560 g         2366 t
ORIGIN      
        1 aggtttacgt aaattagaat acccaatcaa acctcattta atattatttg atgcaagtac
       61 aaccaccggt attgaccttc aaaatacatt tgaaacacta attaagaaat acaatcttta
      121 tgaaactgat agcgggagga aaaacggatt taaaataatt ggatggagta cagtttggga
      181 taatgacgaa cagcaagtcg ataagaagag tggtaataaa aaaaatcgtc tcaaagattt
      241 atttcccgat tattcaaagg aaagcaatgc aaaaaaagag cattttgatt gtttaaagaa
      301 acatcttcac ttatacgatc atgagaaaaa gacctttgag gctgttcgta aatcaatttt
      361 gaacgcactg gtacaagttt tacgattaga aggtattttg aaggctccaa atattcctta
      421 cagaaaaagt tctttaattg agttcatcaa ggatttaggg actgaaactt ataatgattt
      481 tatagcaaaa ttattcaaat ggtgttttga tttagtagta aaaaagaatt ttgaaaaagt
      541 atatatagag ataaaacagt ttatagaaaa tgattttatc aaatggaatt ggctgaatga
      601 aacagatgtt accgcaagaa gtattaaaca tgcaaaggat tttatcgaaa gcaataatta
      661 tacattccaa agtcatttga ctaaagagat tattaatgag ggaaaagaaa ttaatcctta
      721 taatattgag attaattctg ttcatgcagt aaagggtcaa acacattgtg ctactatgta
      781 tgtggaaacc tcttatcatg aatacgaaac aaaaaaacta aaggttattt ctaaagaggc
      841 aacaaagaca aaaccagaag aattattgcc ctatccgctt tttttcaacg agcaatctta
      901 caggcaaaac aaagatgttc gtgcaaaaga agcaataaaa atgatgtatg taggtttttc
      961 tagacctaca catttactgt gttttgctgc cctaaaagat aatattgggg atattaaacc
     1021 ttataaagat gcaggttggg aagttatttc tgatttgatt ttatcatcag tagaaaatgg
     1081 agaaaatgac gaatgagcaa ttccccaaaa agtcaactca ccataaaaga cgaattaacc
     1141 gatttcctac tctatactgc ccccaacgga aaagttaaag ttgaagtctt cctgcataat
     1201 gaaacagtct ggcttaccca agatcagatg gcaacccttt tcggcaaagc aaaatctacc
     1261 ataaatgaac acattaaaaa tatttatgac gaacaagagc ttgaagaatc agccaccatg
     1321 aagaaattcg gaatttccga atttcagcaa aaaacaccca attactacaa tcttgatgta
     1381 ataatatccg tcggttaccg tgtaaaatct ccacagggga cacaatttcg tatctgggct
     1441 acacaacgct taaaagaata catcatcaaa ggctttgcct taaatgatga ccgttttaag
     1501 tcgggtaatt ccatgaatta tttcgatgaa ttacaggatc gcttgcgaga aatacgtctt
     1561 tccgaacggt ttttctatca gaaaattaaa gacatttaca ccaccagtat tgattatgac
     1621 cccaaggatg agaaaacagt tgagttcttt aaaatagtac agaataagct tctttgggct
     1681 atcagtaagc aaactgcagc cgagttagtg tatcgcagag tggatgccag tttaccacta
     1741 cttggtatgc aatcttatga taaaaaggga ttgatttctg ttaaaaagtc cgaggtaagc
     1801 atcgcaaaga attatttgaa tgaagatgaa ataaaattac ttggactttt ggtggagcaa
     1861 tatctcgctt ttgccgaaac tatggcacaa caacgagtgc ccatgtacat gaaagactgg
     1921 attcagcggt tagatattat tttacaactc aatggacgag aactgctcac ccatgcggga
     1981 aaaataagtc atgatgtggc tatagaaaaa gctggtatag aatatgaaaa attcagagat
     2041 gcccaaaaat caattgagaa agaacaaagc ctcaaagaaa tagaagaaga tattaaaaat
     2101 tgggggaaga atagaaaatg actaattccc aaaacaacaa actcaccata aaagacgaat
     2161 taacagactt cctgctctat actgccccca acggaaaagt aaaagttgaa gtcttcctgc
     2221 ataatgaaac agtctggctg acccaaaaag ctattgcaat attatttggc gtacaagttc
     2281 ctgcaattaa caaacatttg gaaaacatct atgaaagcgg ggaacttaat agagagacaa
     2341 ctatttccat tttggaaaca gttcaaatcg aagctcgaaa tacggggtca ataaatgttt
     2401 gaaagataaa gaattcattt cggcccagca agcagaagaa aaagtacttt cagaatatga
     2461 tacggatata aaaaacctat attgcataat atctctatac attaaatttc caggagcatg
     2521 gtttggaaaa aacttatata gcaaatcgtc ttgaaatggt tttaggtgta ccagcaaaat
     2581 tttggataaa tcttgaagca atttatagag aaaagcttct acttatagaa caagaaaacc
     2641 aattgtccgc tgatattcga caataagaaa atgttttttc tcatataggg gtaagaataa
     2701 tgactcaaca gaaattcaat aaaatcttct ggaaacagat gaatatgtga acattaacaa
     2761 aaaaataata cttttattct gtatgaaatg aaaaaggttc tacttaatga gtaaagtaaa
     2821 aattctctac acttctgctt taaattccaa tgggaatcta gttacagcct tgaatgccga
     2881 aaagggcaat gttttcacgt gtccaatttg caaggggcaa atgaatctcc gaaaaagtga
     2941 aagggaaggg ccaggaagtc gtcgccctca ttttgctcat aaagcacttt caccaaattg
     3001 tacccccgaa gggcttttac atcattcatt caaaatgatg tttgccgata ttttaaagaa
     3061 atgtattgaa aatggttggc cttttccatt caaaaaaaag tgtgactatt gtggtaaaga
     3121 gcattgttgg gacttactaa aaagagcaag ggatgttaaa attgaaatgg atcttgggtc
     3181 atgtagacct gatattatgt tactaaacgg aaatggaaat ccttatttag cgattgaagt
     3241 tgtagtatct cattctcctg aagaagaggt tcttgagtat tacagaaaga ataaaatagt
     3301 acttttccaa attaatcttt caacagaagc tgatttgaat aaaatatgtg aacgagcttc
     3361 aaaaccagat gtgtttacgt tctgtacaaa tccaagatgt tcaaaatgtg gcttttttat
     3421 ggataaacgc cttttagcag tttggacatc ggaatgttat agatgtcgat ctccaatgaa
     3481 tattgcaata acaatgacag aaaagaatct tatttctgga gaattaccaa gcgaaaaaac
     3541 gccaaaatat tttttagaaa aagaaattca aatggcgaaa gcaaatggag tttttattcg
     3601 caagcattat agtaaaacta tggattgttc ttacaatgct tgcacatgtc aacgctgtgg
     3661 cgcttttctt ggtgaacatt atatgattga tgaaataatg aatgtggttt atgcatatca
     3721 gggtggcaat aaagacgttt taaagcttat agatattgaa tatttctgcc caaggtgtga
     3781 atgaaaaggt gttatgattt ctaggttttt agcatatctt tctaatgata tcatgaaggc
     3841 cgcttattcc acaaaagctg gatgacaaaa catctaaaac ttcttgattg taccccaaat
     3901 cacataaatc tttgaaaaat cgccaaaatc agcgttatgt attttatctc ttgtctaaat
     3961 atagcaaata tgctatattc catatatgga tttgatcttt ttccccgata cgcacccttt
     4021 tgaccccgat aaatcggaag ccaaagatga gggtggtgat gttgggaaag aaatcaaggg
     4081 tttatctaag tatagacaag atttgtacct gctagtaaaa agttttttga aagccttaaa
     4141 aaaagtgacc gatataacgc cgtatttaca aaacaagcaa atttacaggt tcacacaaaa
     4201 gtacgatggt ctctatgaaa tgcgaattcc caagcagaaa aaaagtggcg tctttagaat
     4261 ttacttttgt ttttcgaaga cgaataggga aaaaataatt ctactctgtg cagaattaaa
     4321 gcaaaagaca aaaccgatga aattagataa cgcattgata aaattaaaac aatatagaga
     4381 agatgaaaac aaagaggtac acaatgaaga acattaacgt attagattac atggattctt
     4441 tagaaaaaga agacaaagat cttgatttgt ttgtaaaaat gtacgaagct ctttcttcgt
     4501 tccttactga ttttgacttt ttgaaagatt ccatgggttt gactcagaaa gatgttgccg
     4561 ataaaatggg aaccacgcaa agtgcaattt ctcgcattgc atcaatgaaa acaaatccat
     4621 cttacaaaca gttacaaaag atggcagaag ctgttggagg tgaactgctc atcactccaa
     4681 tgaaagatat gactgctcaa gtcccttacg acttgcagaa taaggtgaga gagttggcca
     4741 aaggtgaaaa caagaccacg aatgaatatc ttgatgaagt gatcagagat tctattcagt
     4801 cggaatttaa atggtatgca aggcaaacta ttgaagtgaa taaagtttgt gagtgtccta
     4861 cagcgaatgg cattggtttg cgtgattttt ggtctaattc atcaaaatcc tctttaaatt
     4921 ttggggaaat taagaaaaaa actataaggt ttgaatcaca agcaatggcg gtgtaaaacg
     4981 aggtgatgta tgaaagataa ttttcaacaa ctaataaaat tacttcaact gggtgatatt
     5041 cgttttatgg attctcatga atcggtatta cacattccag aaaatatttt accagattcg
     5101 aatacgtcac ttgaatctgg acaagctttc gacaaagaaa gtcctataat aaaaggtgat
     5161 tgtttgttat ttcgagtcaa atatgtgttc tcatttttga ataacaaaga gccatatttt
     5221 aaagcagaat atattgttaa tatatttttc gaggctagcg atataaaaaa ggcagaagaa
     5281 ctgctacaag atgaagaatt gaaaaagata ttcctcgaaa agcaattgac tcatactatg
     5341 tggccaattt tgcgtggaac actcatgaac gcttttagca ggcattctct aaacccttgt
     5401 atgctgccat ggattgtttg aagttcgtga ttttataaat attgattaag aaaatgaata
     5461 tatatttaaa tcaacgttta gtagattaat aaaagaatag ctaaaacatt ggaaggagct
     5521 attaaagcga tttaattaat gaaattcacg gaagaaaaat tagaacaggc ttttacagag
     5581 ttattagaac agcaagggta tactcatcat ttaggtgaat ccattgttcg taacattgaa
     5641 gatgttttaa ttgaagccga tttagaatct tttttaatgg ctaaatatgc tcatgaggga
     5701 atcactctaa acgaaatcaa atctattatt cttcagctca aatcgcttcc tgcttctgac
     5761 ctttacgaga gtaataaaac gtttttaaga atgctttccg atgggtttat tcttaaacga
     5821 gaagaccgta gcaaaaaaga tatttacatt caattcattg attatgcagg gctagatcct
     5881 caacggcaac ctgatccaga acatttgatt aaagtagctt cagaaaataa aaaagagtat
     5941 gatcaggcaa atcaaaccga taagaacatc tacaagtttg taaatcaatt agaaatttta
     6001 gggttagaaa aaagaattcc agatggtatc atctacatca atggcttgcc tttggtggta
     6061 ttagaattta aaagtgccat tcgcgaagat gctactattt ttgatgctta caagcaaata
     6121 accgttcgct accgcagaga cattcctgaa ttgtttaaat acaatacttt ttgcatttta
     6181 agtgatggtg taaacagcaa agcaggttcg ttctttgctc aatacgattt cttttatgcg
     6241 tggcgccgag tggctggttt ggcaaaagac gttgatggca tagattcaat gtatactcta
     6301 atccagggta tgtttcacaa gaatagactg cgcgatatta ttcgcaactt tattttcata
     6361 cccgacaaca gcaagaaaga agaaaaaata gtgtgccgct atccgcagta ttacgctgca
     6421 cgcgccttgt acgataatat tataaaagca caaaagccca ttggcgatgg aaaaggtgga
     6481 acatactttg gcgctactgg ttgtggtaaa agctatacaa tgctttacct cacaaggctt
     6541 ttgatgaaaa gcgaatactt tgaaagcccc actattgtgt taattaccga ccgcaccgat
     6601 ttagacgacc aactttcggc acaattctta aacgccaaaa cgtttattgg cgacaatact
     6661 gttcaaagtg tagaaagccg tgctcattta cgagagcttc ttcaaggtag gcaaagtggc
     6721 ggtgtctttt taacaacgat tcacaaattc actgaagaca cctatctatt aacagaaaga
     6781 agcaatgtca tctgtatttc tgatgaagct caccgaagtc aggtaaatct ggaccaaaaa
     6841 gtaaaagtca cagaaaatgg tgttaccaaa tcatttgggt ttgccaagta tctgcatgac
     6901 tctcttccaa acgccaccta tgtaggcttt actggaacac caattgatgc aacgctagat
     6961 gtttttggaa aagtagtaga tgcatacaca atgaccgaat ctgtaaaaga tgaaattact
     7021 gttcgtatcg tttacgaagg cagagcggca agagtagcac tcaataatgg tgagttggaa
     7081 aaaattgaaa aatactacga agaagctgct gaagctggtg caaatgaata tcagattgag
     7141 caaagcaaaa aagaaacagt aaacatgtat gctatactcg gtgatccaga ccgtttgaaa
     7201 gctgttgctg aagactttgt taatcactat gaaagtagaa tcgcagaagg agcaaccata
     7261 aaagggaaag ccatgtttgt aagcagtagt cgtgaaattg cttatgaact gtataagaat
     7321 ataattgcac ttcgtccgca atggaatgag attcgagaag cagaagaagg agctgtttta
     7381 acagacaaag ataaaagaga attaaaacca attgaacgta ttaagttaat tgcaaccaga
     7441 ggccaagacg atccgaaaga gatgtatgac cttttgggca caaaggatca aaggaaagag
     7501 ctagatcgtc aatttaagaa cgaaaaatcc aattttaaga tagcgattgt cgtcgatatg
     7561 tggcttactg gctttgatgt acctttcctt gatagtattt atattgataa acctatacag
     7621 caacataacc ttatccagac catatcacgc gtcaaccgta agtttgaagg gaaaaataaa
     7681 ggtttagttg tcgattatat aggcatcaaa aaacagatga atttagctct tgcaaaatac
     7741 aataaaggcg atgagcacaa ttttgaagac attgcagaat cgctaataat agtcagaaat
     7801 catttagacc ttcttgcaaa actgtttcat acttttgata attctaagta tttcaatgga
     7861 acaacaatac aacagcttaa tacattgaat tttgctgccg agtttgtcca aaaaactaaa
     7921 gaactagaaa accgtttcat gggcttggta aagcgtctaa aagccgctta tgatatttgt
     7981 gcaggaagcg aaaaactcac acaaatagaa agaaactata ctcatttcta tttagccgtt
     8041 cgttctattg tttttaaact cacaaaaggg aatgcgccag atacttctca aatgaatgca
     8101 aaagttagag aaatgataaa agatgcgtta caaagtgatg gcgttgaaga aatatttaag
     8161 ctcggtgaag aagcagagac cgaacaagac atttttgatg aagattattt ggcaaaaata
     8221 aataaaataa aattaccaaa taccaagatt aagttgttgc aacaattgct tgcaaaagta
     8281 attggag
//
LOCUS       DAOOBB010000079         8009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_10885_length_8009_cov_6.520493, whole genome shotgun sequence.
ACCESSION   DAOOBB010000079 DAOOBB010000000
VERSION     DAOOBB010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 8009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8009
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10885_length_8009_cov_6.520493"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1364
                     /locus_tag="PLT31_04970"
     CDS             <1..1364
                     /locus_tag="PLT31_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94526.1"
                     /translation="RRRLPTQYPAFSCHAKGKGVGFMKKNMMEKDHFLLNQNSLDDDK
                     LYSFYSGTIKKNKLLLTMNPIFLLLTCFFLFYGCSQDQNAGATTETTNGIALFIKDSS
                     NQPLPLAKIALFDSSLSSPFATATSDSNGVVHFTLNDSIKDYFIEAIHGADSSAMAWE
                     KFEGDSSLVQVLSSVEIKVAFNTQDTLLLPNSIYLKGTPYQVISSEDGFIFKRAPQGI
                     FEVRTDNDISLGTFEINQSSSFTFNEDALRKTLLLEDFNDGNIQFKYADKLSASGWYF
                     SSSDDGIWYANENIEWTDPTEEIEHPAQFISENAFEGMSISLHYSTGDSGMLVLGTHL
                     GADSISYDFSDMKAVRLMVRGDAIFDVALESNRTVGDNHYPKVLWRDTASTEWTEYVF
                     YPGNEIVLPEHQQYTWNEVKQEIGIFSIFINSGSFIEIDQIIIDGINEITLEKPIKIS
                     SEP"
     gene            complement(1368..2258)
                     /locus_tag="PLT31_04975"
     CDS             complement(1368..2258)
                     /locus_tag="PLT31_04975"
                     /inference="COORDINATES: protein motif:HMM:NF016903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnan synthesis F family protein"
                     /protein_id="HPW94527.1"
                     /translation="MRTKVLYASYVKGNSIPYYVRYALEALSFEDVSVVYLTNERELD
                     EESIAFLKAKKIELFLTENKGFDFGMWNRYLKSISLEEKKSWDRLCLINDSVIYYKNQ
                     FGFFFSKAENLSADMVSLTCSEELSFHLQSFFLYLKPSAMTVLYAHLEEASESDSFYE
                     VVRNMEIGLSSRMLKCGLKLESLFKTSRNVLFSYDELIKEKAGFIKRKLLERRFTPKE
                     KFHFLRHGGFSALYANYYEMIKKSGDADSSFPIDSIVVHKKTLQNQMKDFFLCLFFYA
                     LINPTEKIKKILKKNKLRKS"
     gene            complement(2259..4406)
                     /locus_tag="PLT31_04980"
     CDS             complement(2259..4406)
                     /locus_tag="PLT31_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HPW94528.1"
                     /translation="MALLPTKDQVIAIIRDESMTGSRLRGALGLPKKLKLSFKQMLAE
                     MVREGLLERTCHKEYILGNSEPSPFKKDTSSLPRPAARSRKKIDPVSTISIKRGVLKL
                     VEKDRWIVIESSTGKEFKVAHRKNAPGKDGQTISFTLYPHPKEKHSYLAKVEEHSMSP
                     EITWKEVKDEFMKSSNLPDSFSQEIEEFAASKKSPTAKDFKGRVDYRGLTILCIDPEG
                     ARDHDDAVSVERLPGGGFKLGVHIADVSYYVPEGSALDEEALNRSYTQYLPWCAVPML
                     PERLSGDLCSLHEGVDRCAFTCMMVLNKKGEVQSYEFHRSVINVTSSISYERAVEMKN
                     EGNTAVADLALVSKLLKENRSKSGILELGGTEYQCLFDEKNEPVQIVPRKNDESNSWI
                     EECMLIANNCCAKELVRRQLQGIFRIHEAPDTKDIMELYYLYPDLFKDAPVELRDLGK
                     PRRGDSNLNPIVFSLYQHLVIRAAGDETLINRILRSMQKAHYDSNSFGHFALNWQDYS
                     HFTSPIRRYADLWCHRELARTPKTEKQERKNSVIEVCDWISANEIKNQKTERIAIKVC
                     ATYLLKDRIGDIFEATINGIEQWGIYVSVKDPMAEGLVRFKDIAGNDYYIFNPEKGFV
                     FSKRGGKTLRRGDKVEVQLLRVNPLRGEADFALLKKLEDEKTRAPGRRPSKKDRAEVA
                     EELGLLKSVDDFTAEEKRNRTRSTKKTFSRKRR"
     gene            complement(4407..5174)
                     /locus_tag="PLT31_04985"
     CDS             complement(4407..5174)
                     /locus_tag="PLT31_04985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPW94529.1"
                     /translation="MSEEPFVLKYNWRHKILAIVYYFMIAVAAFFGGSLYAFFHCYRR
                     NDWHDLTHVCSLFFIPMFKIFGIKMVVEGKENIPSKSGFVMVANHQSFLDINAIFAGV
                     SHTAFLAKADLWKIPYFGWGLSKTGSIPIYRKDPKKNAGMGKIIKQRIDQGYNYCVFP
                     EGNRTKDGKMTSFKNGIFRIAKEHEFTILPITLVDTGKRLSRNTLGLRPGPIRIVVHP
                     PITPEQYKEMSMEELRDSAHDIVESELPYKKQKNEEA"
     gene            complement(5171..6169)
                     /gene="pyrC"
                     /locus_tag="PLT31_04990"
     CDS             complement(5171..6169)
                     /gene="pyrC"
                     /locus_tag="PLT31_04990"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HPW94530.1"
                     /translation="MSTLKLPLPDDFHVHLRQGDALADYARDIACQFGRILVMPNTVP
                     ALSTTESILNYKKAIVSAAPNLEPLMIFKIHSGLTSSDLEAFKAMGVLAGKYYPAGVT
                     TNSEDGVSDFESIFPVVSMMEKMGFVFSVHAEEPNVFCLDRETAFIPKIKTLAQKFPQ
                     LRIVFEHVSTAAAVKAVLEMPSTVAATITVHHLTMTLEDVIGDALRPHHFCKPILKLP
                     SDREAIRKAAFSGNPKFFFGSDSAPHKQEKKECAYGAAGIYSAPVLLPILVREFEKEN
                     ALDKLPNFIAGYGADFYSIPRTTKEIQLVKKTWCVPNAVHGVVPLAAGETLEWSMV"
     gene            complement(6211..6435)
                     /locus_tag="PLT31_04995"
     CDS             complement(6211..6435)
                     /locus_tag="PLT31_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HPW94531.1"
                     /translation="MKISIKVHARSKREQVLGPLPDGSYKVEVKAPPVEGAANEAVCL
                     LLADFFKKPKRAVRVLLGSTNSKKVVEIDD"
     gene            complement(6439..6963)
                     /locus_tag="PLT31_05000"
     CDS             complement(6439..6963)
                     /locus_tag="PLT31_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HPW94532.1"
                     /translation="MELTPLDIRNQTFSTKRFGGINPEEVKAFLETAAQAFEQMSRMR
                     TDLVERLKIAEERVNYYKQIEKTIQDAVMTMQRTVDEVKHSADKEAELIIAEAKARAV
                     REVENTKRQAEEMRNEIEQLKQLRANYFIRTRALIRSQEELLRAMEQDQRSLIEESTP
                     VKRPIPGGSGQVIA"
     gene            7245..7508
                     /locus_tag="PLT31_05005"
     CDS             7245..7508
                     /locus_tag="PLT31_05005"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HPW94533.1"
                     /translation="MPHFVYMLECAGNRIYTGYATDVEKRYQKHCEGKGAHFTKVFPP
                     QRILKIFETETKSEALSLEFKIKQLPKPKKELLILGQLEISFP"
     gene            7592..8005
                     /locus_tag="PLT31_05010"
     CDS             7592..8005
                     /locus_tag="PLT31_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003110349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPW94534.1"
                     /translation="MWIILAIASAFFAALTSILAKIGIEGMSSNLATAIRTVVVLVMA
                     WAIVFISGAHSGIHNITQKSWLFLILSGIATGLSWLCYFKALQLGDASRVVPIDKFSV
                     VITMVLAFTILGEAMTAKTIVGGALITLGTFVLIL"
BASE COUNT         2407 a         1627 c         1369 g         2606 t
ORIGIN      
        1 tgcgacgccg tttaccaact caatatccag ctttttcctg tcatgcaaaa ggaaaaggag
       61 ttggattcat gaaaaagaac atgatggaaa aggatcattt tttattaaat caaaacagtc
      121 ttgatgatga taaattatat tcattttatt caggtactat taaaaagaat aaattgctac
      181 ttacgatgaa tcccattttt ttattactca cttgtttctt tcttttttat ggttgcagcc
      241 aagatcaaaa tgcaggagca accacagaaa caactaatgg gattgctcta tttattaaag
      301 actcgagcaa ccagccactc cccttggcta aaatcgctct ttttgactct tctctttctt
      361 ctccttttgc cactgcaacg tctgattcta atggagtcgt tcattttact cttaatgatt
      421 ctataaaaga ctatttcatt gaagccattc acggagccga ttcctctgca atggcttggg
      481 aaaaatttga aggcgatagt tcattagttc aggttctttc gtctgtagaa atcaaagtgg
      541 cttttaatac tcaagatact cttcttttac ctaattctat ttatttaaaa ggaactcctt
      601 atcaagttat ttccagtgaa gatggtttta ttttcaaacg tgctcctcaa ggtatttttg
      661 aagtccgtac tgataatgat atttcgctag gcacttttga aatcaatcaa agtagttctt
      721 ttacttttaa tgaagacgcc ttaagaaaaa ccttattact agaagacttt aatgatggaa
      781 acattcaatt taaatatgca gacaaactct ccgcctctgg ttggtatttt agttcttccg
      841 atgacggcat ttggtatgct aatgaaaata tagaatggac agaccccact gaagaaatag
      901 aacaccccgc tcagtttata tcagaaaatg cttttgaagg catgtctatt tctcttcact
      961 attctacagg cgattctgga atgctcgtat taggaaccca ccttggtgca gactctataa
     1021 gctatgattt ttctgacatg aaagcagtcc gattaatggt tcgtggcgat gctatttttg
     1081 atgttgcttt agaatctaat cgcaccgttg gagataatca ttatccaaaa gtcctatggc
     1141 gcgataccgc ttctacggaa tggacagaat atgtatttta tccaggaaat gaaattgttc
     1201 tccctgaaca ccaacaatat acctggaatg aagtaaagca agaaattggt attttcagta
     1261 tttttattaa tagcgggtcg tttattgaaa ttgatcaaat tatcatcgac ggcatcaatg
     1321 aaataacgct tgaaaagccc attaaaattt cttctgaacc ttaaaagtca agactttctt
     1381 aacttatttt ttttcaagat ttttttaatt ttttccgttg gattaattaa agcgtaaaag
     1441 aataagcata agaaaaaatc tttcatttga ttttgaagag tttttttatg aactactatg
     1501 gaatctatag gaaaagaaga atcagcgtct ccagattttt ttatcatttc ataataatta
     1561 gcatatagcg cagaaaagcc gccgtgcctt aaaaaatgaa atttttcttt tggagtaaat
     1621 cgacgttcca aaagtttgcg cttaataaac cctgcttttt cttttatcag ttcatcataa
     1681 gaaaacaata cgtttcgaga tgttttaaaa agagattcta gtttgaggcc acacttaagc
     1741 attcgagaac ttaaaccgat ttccatatta cgcacgactt cgtaaaaaga atccgactca
     1801 gaagcctcct ctaaatgcgc atataaaacc gtcatcgctg aaggtttgag gtacaagaaa
     1861 aacgattgta gatgaaaaga aagttcttcg ctacaagtca atgaaaccat atctgcagaa
     1921 agattttcgg ctttagaaaa gaaaaatcca aactggtttt tataataaat cactgaatcg
     1981 tttattaagc ataagcgatc ccaggatttc ttttcttcta atgaaatgct ttttaagtag
     2041 cggttccaca ttccaaaatc aaacccttta ttttctgtta aaaagagttc aatctttttt
     2101 gcttttagaa aagcaatcga ttcttcgtct aattctcttt cattagttaa ataaacaacc
     2161 gaaacatctt caaaagaaag tgcttctaat gcatagcgaa cataataagg aatactatta
     2221 ccctttacgt aagaagcata aagcactttc gttctcaatc aacgcctctt tcttgaaaaa
     2281 gtcttcttag tagaacgcgt ccgatttcgt ttttcttctg ccgtaaaatc atccacagac
     2341 ttgagtaaac cgagttcttc tgcaacttca gcgcgatctt ttttcgaagg acgacgtcca
     2401 ggagcacgag ttttctcatc ttctaatttt tttagtagag caaaatcagc ttcaccgcga
     2461 agaggattca ctcgaagtaa ctgcacttcg actttatcgc cgcgacgcaa tgtttttccg
     2521 cctcttttgc taaagacaaa acctttttct ggattaaaga tgtaataatc attgcccgca
     2581 atatctttga aacgaacaag cccttcagcc attgggtctt tcactgagac ataaatgccc
     2641 cattgctcaa tgccattaat cgtcgcttca aaaatatctc caatacgatc ttttaaaagg
     2701 tatgtagcac aaactttgat tgctatgcgt tctgtttttt gatttttaat ttcatttgca
     2761 gagatccaat cacatacttc aatcacgcta ttcttacgtt cttgtttttc tgtctttgga
     2821 gttcttgcga gttctctatg acaccataaa tccgcataac gacgaatggg tgatgtgaaa
     2881 tgagaataat cttgccaatt caaagcaaaa tgaccaaagc tgttgctatc ataatgagct
     2941 ttttgcatgc tccttaaaat tcgattgata agcgtttcat cgccagcggc tctaatcact
     3001 aaatgttgat atagtgaaaa gactattgga tttaagtttg aatcaccacg acgaggtttc
     3061 cctaaatctc gaagttccac tggagcgtct ttgaaaagat cagggtacaa ataataaagt
     3121 tccatgatgt cctttgtatc tggagcttca tggattcgga aaattccctg tagctgtcga
     3181 cgcactaact ctttggcaca gcaattattt gcaatcagca tacattcttc aatccaagaa
     3241 ttggactcat cattttttct agggacaatt tgaacaggct cattcttttc atcaaataag
     3301 cattggtact ctgtgccgcc aagctctaaa ataccgcttt tggagcggtt ttcttttaat
     3361 aatttggaga ctagtgctaa gtcagccacg gcagtgttgc cttcgttttt catctctacg
     3421 gcacgttcat aactgataga agaagtcacg ttgatcacgc ttcgatgaaa ttcatacgat
     3481 tggacttcgc cttttttatt taagaccatc atacatgtaa aagcacaacg atcgactcct
     3541 tcatgtaaag agcataaatc tcctgaaagt cgttctggta gcatgggcac agcacaccaa
     3601 ggtaaatatt gggtgtaact acgatttaat gcttcttcat ctaaggcgct cccttcaggg
     3661 acataataac ttacgtcagc gatatgaacg cctagcttaa aacctccgcc tgggaggcgt
     3721 tccacagaaa ccgcatcatc atgatcgcga gctccttctg gatcaatgca aagaatggtt
     3781 aaacctcgat agtctacgcg gcccttaaaa tcttttgctg ttggactctt tttcgaagcg
     3841 gcaaattcct caatttcctg actaaaagaa tcaggtaaat ttgagctttt cataaactcg
     3901 tcttttactt ccttccacgt aatctcagga gacatggaat gttcttccac tttagcaaga
     3961 taagaatgct tttcttttgg atgtgggtaa agcgtgaaag aaatcgtttg accatctttg
     4021 cctggcgcat tttttcgatg tgctacttta aactcttttc ccgtagaaga ctctataaca
     4081 atccagcggt ctttttcaac taattttaag acgcctcttt ttatggaaat agtggaaact
     4141 gggtctatct tcttacggct tcgagcagca ggtctcggga gagaactagt atctttttta
     4201 aatggagatg gttcactatt cccaagaata tattccttat ggcatgttct ttccaataac
     4261 ccttcacgaa ccatttcagc aagcatttgc ttaaatgata attttaattt cttaggcagg
     4321 cctaatgcgc ctcttaagcg actccccgtc atagactcat cgcgaataat cgcaatcact
     4381 tgatcttttg tcggtaacaa agccatttag gcctcctcat ttttttgttt tttataaggc
     4441 aattcacttt ctacaatatc gtgagcagaa tctcgtagtt cttccataga catctcttta
     4501 tactgttctg gagtaatagg aggatgcact acaatacgaa taggcccagg tcttaatcca
     4561 agagtattcc tagataatcg cttaccagta tccactaaag tgattggaag aattgtaaat
     4621 tcatgttctt tggctattct aaaaatgccg tttttaaatg aagtcatctt cccgtctttg
     4681 gtgcgattac cttctggaaa gacacaataa ttatagcctt gatcaatacg ctgtttgatt
     4741 atttttccca tgcccgcatt ttttttaggg tcttttcgat aaataggaat agatccagtt
     4801 ttacttagtc cccaaccaaa atacgggatt ttccaaagat cggcttttgc taaaaacgca
     4861 gtatgactca cgcctgcaaa aatagcattg atgtctaaaa aactctgatg gttagcgacc
     4921 atcacaaaac cacttttaga tggaatattt tctttccctt cgacgaccat tttaattcca
     4981 aaaattttaa acattggaat aaaaaataga ctgcaaacat gagttaaatc atgccaatca
     5041 tttcggcgat aacaatggaa aaaggcgtat aaagagccac cgaaaaaagc agctacagca
     5101 atcatgaaat agtatactat cgccagtatt ttatgacgcc aattatattt tagtacaaaa
     5161 ggttcctcac tcataccata ctccattcca gagtttctcc tgctgctaaa ggaacaacgc
     5221 catgaactgc attaggtaca caccaagtct ttttaactaa ttgaatttct ttcgttgttc
     5281 taggtataga ataaaaatca gctccataac ctgcaataaa attgggaagc ttatcaagag
     5341 cattttcttt ttcaaactca cgaactaaaa taggcaaaag cacgggagca gaatatattc
     5401 cagcagcacc gtacgcacac tctttcttct cttgcttgtg cggcgcacta tcagagccaa
     5461 aaaagaattt tgggtttccg ctgaatgccg cttttcgaat cgcttctcga tctgaaggaa
     5521 gctttaaaat cggcttacaa aaatgatgtg gtcttagcgc atcaccaatc acatcttcca
     5581 aagtcatcgt taaatgatga accgtgattg tcgcggctac tgtagaaggc atttctaaaa
     5641 ccgctttcac agctgctgct gtagaaacat gttcaaaaac aattcttaat tgtggaaatt
     5701 tttgagctag cgtttttatt ttcgggataa aagcagtctc tcgatctaaa caaaaaacat
     5761 ttggttcttc agcatgaacg gaaaatacaa agcccatttt ttccatcata gaaacaacag
     5821 gaaatatact ttcaaaatcg ctgacaccat cttcactatt agtcgttact ccagcaggat
     5881 aatatttgcc agcaagcaca cccatcgctt taaatgcttc taaatcactg gacgttaaac
     5941 cagaatgaat tttgaaaatc atcaaaggct caagatttgg cgccgccgac acaattgcct
     6001 ttttgtaatt aagaatactt tctgttgtag aaagagcagg caccgtgttt ggcatcacaa
     6061 gaatgcgacc aaattggcaa gcaatatctc ttgcataatc ggctaacgca tctccttggc
     6121 gaagatgcac atggaaatca tcaggtaaag gtaattttaa ggtagacatc acccttgtaa
     6181 aataagtaaa tctgacaaag aaggaaatat ttaatcgtct atttcaacga cttttttgga
     6241 attagtagaa cctaatagaa ctcgcaccgc tctttttggc tttttaaaaa aatctgctaa
     6301 caataaacag acggcttcat tcgccgcccc ctccacagga ggtgctttta cttctacttt
     6361 atatgatcca tcaggaagag ggcctaaaac ttgttccctc ttgctacgag catgcacctt
     6421 aatactgatt ttcatagatc aagcaataac ctgaccactt ccaccaggaa tagggcgttt
     6481 tacgggagta gattcttcga tcaaagacct ttggtcttgt tccatggctc tcagtaattc
     6541 ttcttgactg cgaattagtg ctcgtgtgcg aataaaatag tttgctctaa gttgttttaa
     6601 ttgctctatt tcatttcgca tttcttctgc ttgacgcttt gtattttcta cttcgcgaac
     6661 ggcacgtgct tttgcctctg caataatcaa ctcggcttct ttatcggcac tatgcttgac
     6721 ttcatcgaca gttctctgca tggtcatcac agcatcttga attgtttttt cgatctgctt
     6781 ataataattc actcgctctt cagcaatttt aaggcgctcg actaagtctg ttctcatgcg
     6841 agacatttgt tcaaatgctt gggcggctgt ttctaaaaat gcctttactt cttcaggatt
     6901 aataccccca aaacgtttag tactaaaggt ttgattgcga atatccaaag gagtgagttc
     6961 catttttacc taccaaaata aatacttttt agaaaatata taaaatctat tatgattcat
     7021 accagttttg attgtttcag gaaccatagt atggctttga tttcatccac aatctcgaaa
     7081 aaactctttc caactctttt ttgatacatc ctatgcttat aaaagcccca aaaataagga
     7141 aaccccttat aacaaccctg tttatagatt aaatggcttt cgatttatga cctctttatg
     7201 attattattt ctgttttctc attttaactg tttatattag tcacatgcct cattttgtat
     7261 atatgcttga gtgtgctggt aaccgaattt atactggcta tgcgacagac gtggagaagc
     7321 gttatcaaaa acattgcgaa ggcaaagggg cccactttac aaaagtgttc cctccacaga
     7381 gaattcttaa aatttttgag acagagacaa agtcagaagc actatctctt gaattcaaaa
     7441 tcaaacaact ccccaagcct aaaaaagagc tcttgatttt aggacaactg gaaatatctt
     7501 ttccgtaaac gttaaccact atatttagtg aaagcaacaa aagtaattca atctctcttt
     7561 ctatattgtt ttctttaaca agaggattat tatgtggatt atactagcga ttgcttctgc
     7621 attttttgca gcacttacat ctattctcgc taaaattggg attgaaggca tgagctctaa
     7681 ccttgctaca gcgattcgta ccgttgttgt tttagtgatg gcatgggcga ttgtttttat
     7741 cagcggagct catagtggta tccataacat tacccaaaaa tcatggcttt ttttaatcct
     7801 ttcaggaatt gccactgggc tttcttggct ttgttatttt aaggcactac aacttggaga
     7861 cgcgagtcga gtagtgccaa ttgataaatt tagcgtcgta attacgatgg tacttgcttt
     7921 taccatttta ggagaagcga tgacggcaaa gacaattgtc ggcggcgcct taattacgct
     7981 cggtactttt gttttaattc tctaattca
//
LOCUS       DAOOBB010000080         7963 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_10962_length_7963_cov_6.134294, whole genome shotgun sequence.
ACCESSION   DAOOBB010000080 DAOOBB010000000
VERSION     DAOOBB010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 7963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7963
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10962_length_7963_cov_6.134294"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PLT31_05015"
     CDS             <1..385
                     /locus_tag="PLT31_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94535.1"
                     /translation="VVASMIDSLGEESRKMEAYKINSDLVKAEGHPNMIFNELDTLSL
                     QVTYGLRDFINKASDKEDFRFMVRLGYPVLQPNDPAYTDYINTEGDSIYFFFSHFDYV
                     YYDLAPSLEKPMTLKLWLATKRGEE"
     gene            385..1635
                     /locus_tag="PLT31_05020"
     CDS             385..1635
                     /locus_tag="PLT31_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94536.1"
                     /translation="MKTLLFALGCLFTVSSYASSVMGLDALGAEDVSGASAAIAGSGY
                     AGNAKASEEGVSMMNPARLAYNKNVSFSANLSYQMVSASVKNGSLGTQSITIPSFYLA
                     FPMGQFGAFSLGLWQHYSSNIKLELSDSLNSQQATLEYQGSIFEFTPSYAIKLPFWHR
                     LSVGGTAHFVMGNNKRLLTLGPDNSAVAEEDSWATNNSDVTDAVNGSWSILNHPAYYT
                     LAAQYRGKTASIFFSYTTAYTLVNDMEYQLQMSQLDTLAPTKLSRQIDVPITLATGVS
                     YRLPKRQFVMFDFMWKPWKNDIPNVAGSWDLPNETLVESEFLISVGYQRDGSTIFYDS
                     FISRMTYRAGAWYKNGYLKDVSEVGGSIGLGFPLGKRGTTIDLAIQGGVRSSKSDAYF
                     DETFIGVKIGLMGIGAWGNQRSGM"
     gene            1652..2800
                     /locus_tag="PLT31_05025"
     CDS             1652..2800
                     /locus_tag="PLT31_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HPW94537.1"
                     /translation="MAITKKTTAKESVKKVASKIVEKIMPKTKKKETAVKAESKKKEA
                     VVKAETKKKETSVKVESKKPAPVSTKAKGTKVKKEEPKKAGLKKTEVKKAETKKVAVK
                     KEAAKKETVKKVESKKTSTSKKEEAAHSFAPEYLVMMQKDPNWLQAFWEVADSRLKKV
                     KAGKEKLVLRLFDISSDLTVRRSKKRVFKDIEIPADAKSWYVQNHNKEGGFRAAIGTA
                     SGSEFTPIIETGDMQTFSFEGIRPNTENMFFKASLGTAKIGGFNSSGLSSVSAESWLE
                     QLASSSESMFSGTLSSGALASNVSKDSVNYGKDFFLWVKTRLIVYGGTRPDAHLQVRG
                     EPFPLNADGTFSLEMDLPDSTQVIPVFATDKDGDFPTTIVPIVVKRTE"
     gene            2811..4376
                     /locus_tag="PLT31_05030"
     CDS             2811..4376
                     /locus_tag="PLT31_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HPW94538.1"
                     /translation="MGAPGKLLFLLHAHLPFVRHPDYERFLEENWLFEAISETYLPLV
                     QVLFRLLEKGVPGTINLSVSPPLLEMLSDPLLIERYSKHLYKQLELIEKEMIRFEADA
                     EKIPVIRFYHSRQKALIHTWEKVCQKNLLTAFKSLEDSKILTLLTCVGTHPFLPAYQS
                     DPDSIRLQLEVTSRSFETAFGKKPKGVWLPECGYFDGLDSLLAEYGFEYFFLETHGVL
                     LAEPPPTYGVFAPIKTPAGLYCIGREQSSSVEVWSRKSGYPGHPEYREFFRDIAFERE
                     RKYLGEYFFAGESPIETGLKYYRITGGEHKMIYRPWNAMRLAEDHARLFIANREATFT
                     DLIPHVEGHEASILSPYDAELFGHWWFEGPLFIEALFEKAATSSILELASVETICKTP
                     DSSTHRPVFSSWGEGGFSSVWMNDEVQDAYPRFFKMKSLYNHLRSLKKTKRLHRIITQ
                     MAREILLFQSSDWAFMIHNHSAEGYAKSRLDEHYKNVLSLYDLALSKQSDLKELDRLE
                     KKNNLFPWIGELL"
     gene            complement(4347..6107)
                     /locus_tag="PLT31_05035"
     CDS             complement(4347..6107)
                     /locus_tag="PLT31_05035"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPW94539.1"
                     /translation="MNTNGAIIVQSNLEILVEIHHPQYEEARDAIAPFTELVKSPEHL
                     HTYKISHLSLWNAASTGLTAKNVLERLEVQSRYPLPPSVITEIEDYMGRYGILRLVKE
                     EDKLILESDNELLFLEIVKIPEIKPLVESFINEKRAYVNANRRGLLKMHLTNSGFPVE
                     DLAGYISGDPLPISLRKETLSGKEFVLRDYQKEAAQIFYASGSEKGGSGVIVLPCGSG
                     KTVIGMATMALTQTKTLILTPNISASRQWIRELIDKTTLTKDQIKEYSGEVKEIGPVT
                     VATYQILTQRKRKKTNENPDELEEKEEEATFDKDEMVNIPLFNTHHWGLMIYDEVHLL
                     PAPVFRLSTEMQATRRLGLTATLVREDGKETEVFSLIGPKKYDVPWRVLETQGWIAKA
                     DCNELRVSMDPELKIKYALASKREKITISSTNEKKIEVVKTLLNHFNGEEDRILIIGQ
                     YIEQLEKIAEEIQVPLITGKTPNTERDQLYQAFRDGVLKHLIISKVGNFAIDLPDANV
                     LIQISGTFGSRQEEAQRLGRVLRPKSDGGSAHFFTLVSQDSREQEFAMNRQLFLTEQG
                     YAYNIIKDEDWLEKFANPRK"
     gene            complement(6161..6233)
                     /locus_tag="PLT31_05040"
     tRNA            complement(6161..6233)
                     /locus_tag="PLT31_05040"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6198..6200),aa:Thr,seq:cgt)
     gene            6396..7064
                     /locus_tag="PLT31_05045"
     CDS             6396..7064
                     /locus_tag="PLT31_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW94540.1"
                     /translation="MAENTHKEFFIAYGSKIITAVVVVLILVAVVVQINQSNKGTRKQ
                     QTELLGQGLNFIYDGDDEKALVEFERLIQNQEVSGLAFAKAALLAGNIKYKKGDFDGA
                     AVLFQKSLDNAADVDLIAAAAMHGRASVAIEKGDFASAVGLLEKFVAKYGERTGDLSD
                     RYRKAEPVDAVPSVPDALWKLALVYQQMGANDKAKATAEKLLKVYGDSQIYSDKAKKF
                     IASL"
     gene            complement(7235..>7963)
                     /locus_tag="PLT31_05050"
     CDS             complement(7235..>7963)
                     /locus_tag="PLT31_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HPW94541.1"
                     /translation="EWITAQCPKAFAPSWQKKIHLDNDDIFVDSMLADTYLRLLGLSP
                     ITDVQKVRRSLKKVYEMNYKANSPLIGAANLVHVDGSPLDEFNFQAHDVWIGIQFSLI
                     TAMLMHGLKSEAEDLIESMVKNLYNEARIPFAAPEGFNGSCRLHPETIEKNLNLSKAS
                     AAKLHKNLLSAKILLPDSRVSPMLTRDLATFKKTYKSLIQTAKVDETALFSLIHNTAL
                     KYTAGKYFRPGMVFAIVQAAKFMK"
BASE COUNT         2366 a         1407 c         1623 g         2567 t
ORIGIN      
        1 ggtcgttgct tctatgatag atagcttagg agaagaatca agaaaaatgg aagcatataa
       61 gataaattct gatttagtaa aagccgaagg tcatccaaat atgattttta atgaattaga
      121 tactttatct ctacaagtga catatggttt gcgcgatttt ataaataaag caagtgataa
      181 agaagatttt cgttttatgg ttcgtttagg ttatcctgtg cttcagccaa atgatcctgc
      241 ttataccgat tatataaata cagaaggtga ttctatctac ttcttctttt ctcattttga
      301 ttacgtttat tatgatttag ctccctcttt agagaaacca atgactctaa agttatggct
      361 tgcgactaaa agaggagaag aataatgaaa actttattat ttgctttagg ttgtttattt
      421 acagtttctt catacgcttc ttcggttatg ggtcttgatg cattaggtgc agaggatgtc
      481 agtggtgctt cggcggcgat tgctggttct ggctacgctg gtaatgcaaa agcttctgaa
      541 gaaggcgtgt ctatgatgaa tccagcacgt cttgcttata acaagaatgt ttctttttct
      601 gcaaatcttt cttatcaaat ggtatcagct tcggttaaaa atggatctct tggtacgcag
      661 tcgataacca ttccttcatt ttatctagcc ttcccgatgg ggcagttcgg tgctttttct
      721 ttagggcttt ggcaacatta ttcttctaac attaaattgg aattaagtga ttctttaaat
      781 tcacaacaag caacacttga atatcaagga agtattttcg aattcactcc atcttatgcc
      841 attaaacttc ctttttggca tcggttatct gtaggtggta cggctcattt tgtaatggga
      901 aataacaaac ggttattgac tctcggtcca gataatagtg cggttgcaga agaggattct
      961 tgggcaacta ataattcaga tgtaacggac gctgtgaacg gttcgtggtc tattttgaat
     1021 catcctgcct actatactct tgctgctcag tatcgtggta aaacggcaag tatttttttc
     1081 tcttatacaa cggcttacac tttagtaaat gatatggagt atcagttaca gatgagtcaa
     1141 ttagatactt tagctcctac aaaattatca aggcaaatag atgttccaat aacacttgct
     1201 actggcgttt cttatcgatt acctaaacgt caatttgtaa tgtttgattt tatgtggaaa
     1261 ccatggaaaa atgatattcc aaatgtggct ggcagttggg atttgccaaa tgaaacactt
     1321 gttgaatccg aatttttaat ttctgttggc tatcaacgtg atggaagtac tattttttat
     1381 gatagtttta tatcacgtat gacttatcga gcaggtgctt ggtataaaaa tggttacttg
     1441 aaagatgtct ctgaagtcgg tggttctatc gggcttggtt ttccattagg gaagcgtggc
     1501 acaacgattg atcttgccat acaaggaggc gttcgctctt caaaatcgga tgcttatttc
     1561 gatgagactt ttattggtgt gaaaataggg ctcatgggaa taggagcttg gggcaaccag
     1621 cgctcaggca tgtaaaagaa aggagtttta gatggctata actaagaaga caacagcaaa
     1681 agaatcagtt aaaaaagtgg catcaaaaat tgttgaaaaa ataatgccaa aaacaaaaaa
     1741 gaaagaaaca gctgtaaagg ctgaatcaaa aaagaaagaa gctgttgtga aagctgaaac
     1801 aaaaaagaaa gaaacgtctg tgaaggttga atcaaaaaag cctgcgcccg tttcaacaaa
     1861 agcaaagggg acgaaggtga aaaaagaaga accaaaaaaa gcgggtctta aaaaaactga
     1921 agtaaagaag gcggagacta aaaaagtagc cgtaaaaaaa gaagctgcta aaaaagaaac
     1981 tgtaaaaaaa gtcgaaagta aaaaaacatc gacttcaaaa aaagaagaag ccgctcattc
     2041 ttttgctcct gaatatttag tgatgatgca aaaagatcct aattggctac aagctttttg
     2101 ggaagtggca gactctcgtc tcaaaaaagt aaaagcaggg aaagaaaagc ttgttcttcg
     2161 tctttttgat atttcttctg acttaactgt tcgtcgttca aagaaaagag ttttcaaaga
     2221 cattgaaatt ccagccgatg caaaatcatg gtatgttcaa aatcataata aagaaggtgg
     2281 ctttagggct gctatcggta cagcttctgg aagtgagttc actccaatta ttgaaacggg
     2341 tgacatgcaa acctttagct ttgaaggaat tcgcccaaat acagagaaca tgttctttaa
     2401 ggcttcttta ggaactgcca aaattggtgg ttttaatagt tcaggccttt ctagtgtttc
     2461 agccgaatct tggcttgaac aactcgcaag ttcttctgaa tcgatgttct caggcacgct
     2521 ttcaagtggc gctttagcat ccaatgtttc aaaagactcc gtgaattatg gaaaagactt
     2581 tttcttgtgg gtgaaaacgc gtttgatcgt ttatggcggc actcgtcctg acgctcatct
     2641 tcaagtcaga ggagagccat ttccactgaa tgctgatgga actttttctt tagaaatgga
     2701 tttaccagat tctacacaag tgattccggt ttttgcaact gataaggatg gcgattttcc
     2761 aacgacaatc gttcctattg ttgtaaagcg tacggagtaa tcttgttttt atgggtgctc
     2821 ctggtaagct tttatttctt ttgcatgcac atttgccatt tgtgcgtcat ccagattacg
     2881 aacgatttct agaagaaaat tggctctttg aagccatttc agaaacatac ttgcctttag
     2941 tgcaagtctt gtttcgtctt ttagaaaaag gcgttccagg aacaatcaat ttaagtgttt
     3001 cacctccttt gctagagatg ctttcggatc ctttgttgat cgaacgctac tcgaaacatt
     3061 tatataaaca gctggaactg atcgaaaaag aaatgatccg ttttgaagcg gacgctgaaa
     3121 aaatacccgt gattcgtttt tatcatagtc ggcaaaaggc tttgattcat acatgggaaa
     3181 aggtttgtca aaaaaatctt cttactgctt ttaagtccct agaagattct aagattttaa
     3241 ctctactcac ttgtgtgggg acgcatccat ttttacctgc atatcaaagt gatccagatt
     3301 ctattcgcct gcaactcgaa gtcacttcgc gaagttttga aactgctttt ggaaaaaagc
     3361 caaaaggggt atggctccca gagtgtggtt attttgatgg cttagattcg ttgcttgctg
     3421 aatatggttt tgagtatttc tttttagaaa ctcatggcgt tttattagca gaacctccac
     3481 caacatatgg tgtatttgct ccaataaaaa cacctgctgg tctttattgt attggaaggg
     3541 aacaatccag ttcagtagaa gtatggagcc gtaaatcagg gtatcctggg catccagaat
     3601 atagagaatt ttttagagac attgcttttg aacgagaacg aaaatactta ggtgaatatt
     3661 tctttgctgg agaatcgcct attgaaacag ggctaaagta ttatcgaatt actggcggag
     3721 agcataaaat gatctatcgt ccatggaatg cgatgcgcct tgctgaagat catgctcgct
     3781 tatttatagc taatcgtgaa gcgacgttca ccgaccttat tcctcatgtg gaaggccatg
     3841 aagcaagtat tttaagccct tatgatgcag agcttttcgg gcattggtgg tttgaaggcc
     3901 ctttatttat agaagcttta tttgaaaaag cggcaacttc ctctatttta gaacttgctt
     3961 ctgtagaaac tatctgtaaa acacctgatt cttctacgca tcgccctgta ttttcttctt
     4021 ggggcgaagg tggctttagt agtgtctgga tgaatgacga agtacaagat gcttaccctc
     4081 gtttctttaa gatgaaatca ctttataatc atttgcgttc attaaagaaa acgaaacgat
     4141 tacatcgtat catcacacaa atggcgcgtg aaattttatt gttccaatct tcggactggg
     4201 cttttatgat tcacaaccat tctgctgagg gttatgccaa atcacgttta gacgaacact
     4261 ataaaaatgt gctttcactt tatgatttgg ctctttcaaa acagtcggac ctaaaagaat
     4321 tagatcgctt agaaaaaaag aataatctat ttccttggat tggcgaactt ctctaaccaa
     4381 tcttcatctt tgatgatatt gtaagcgtac ccttgttctg ttaagaatag ttgcctattc
     4441 atcgcaaatt cttgttcacg tgaatcttga gaaactagtg taaagaagtg tgcagaaccg
     4501 ccatcggatt tgggtctgag aacacgacct aagcgctggg cttcttcttg gcggctgcca
     4561 aaagtaccag atatttgaat taaaacattt gcatctggta aatcaattgc gaaattaccc
     4621 actttagaaa taatcaagtg ttttaataca ccatcgcgga acgcttggta cagttggtct
     4681 cgttcggtgt ttggagtttt tcctgtgatt agaggcactt gaatttcttc agctattttt
     4741 tcaagctgtt caatatattg tccaataatc agaatgcgat cttcttcacc gttgaagtga
     4801 tttagtaaag ttttaacgac ttctattttt ttctcattag tactagaaat cgtgatcttt
     4861 tcgcgtttag aagcaagagc gtattttatc ttgagttcag gatccatact cacacgaagt
     4921 tcattacagt ctgctttggc gatccaacct tgggtttcta aaacgcgcca tggaacatca
     4981 tatttttttg gcccaattaa tgaaaatact tctgtttctt taccgtcttc tcgaacaaga
     5041 gtcgctgtta agccaagtct tcttgtcgct tgcatttctg tactgagtct aaaaacggga
     5101 gcggggagta agtgcacttc atcataaatc attaagcccc aatgatgggt attgaagaga
     5161 gggatgttta ccatttcatc tttatcaaat gtggcctctt cttccttttc ttctaattca
     5221 tcgggatttt catttgtttt tttgcgtttg cgttgcgtta atatttgata cgtcgcgacg
     5281 gtcactgggc caatttcttt gacttcgcca gaatattctt tgatttgatc ttttgtaaga
     5341 gtagtcttat caatgagttc tcgaatccat tgtcttgatg cagaaatatt cggtgttaag
     5401 attagcgttt tagtttgagt tagagccatc gtcgccatgc caatgactgt tttccctgat
     5461 ccacagggaa gaacaattac accagaacca cctttttctg aaccagaagc ataaaatatt
     5521 tgggccgctt ctttttggta atctcgcaac acaaattctt tcccagaaag agtttccttg
     5581 cgaagcgaaa taggaagagg atctccagag atataacccg ctaaatcttc tacagggaaa
     5641 ccagaattag ttaggtgcat tttcaaaaga ccgcgtcgat tggcattgac gtaagctctt
     5701 ttttcattga taaaagattc tactagtggt ttgatttctg gaatttttac aatttctaaa
     5761 aacaatagtt cattgtcaga ttcaagaatg agtttgtctt cttctttcac taagcgaaga
     5821 atgccataac gacccatata atcttctatt tctgtaatta cagaaggcgg taatgggtag
     5881 cgactttgaa cttctagtcg ttctaaaacg ttttttgcag taaggccagt agaagctgca
     5941 ttccaaagag acaagtggga tattttatat gtatgcaagt gctctgggct ttttactaat
     6001 tcggtaaatg gagcgatagc atcacgagcc tcttcatact gaggatgatg gatttcaacc
     6061 aagatttcta ggttgctttg aacaataatt gcgccgtttg tattcatgtt gcccatattt
     6121 agtaataaaa aagcccgagt cccagaggga atcaggctag agccacccag cagacttgaa
     6181 ctgccgacct gctgattacg aatcagctgc tctaccagct gagctagagt ggcaaaatta
     6241 actcgaataa tctaaaatcg gttgtgaatt tagctatctc aatctagcac gtcaagtttt
     6301 attttgaaaa cgcacttata aatagagctt tgcccctaga ttaaatagtt gaaattttat
     6361 atcttttggt tttaattttt caacatggat taaatatggc ggaaaatacc cataaagaat
     6421 tttttatcgc ttacggctca aaaataatta cagctgtagt tgttgtattg attttagtgg
     6481 cagtggttgt gcaaatcaat cagtccaata aagggactag aaagcaacaa actgaacttt
     6541 taggtcaagg tctcaatttt atttatgacg gtgatgatga aaaagctctt gttgagtttg
     6601 aacgtttaat tcaaaatcaa gaagtatctg ggcttgcttt tgctaaggca gcattgcttg
     6661 ctggaaacat taaatacaaa aaaggtgatt ttgatggtgc tgctgtcttg ttccaaaaaa
     6721 gtttagataa tgcagctgat gttgatttaa ttgctgctgc tgcgatgcat ggtcgtgctt
     6781 cagtagccat tgaaaaaggc gacttcgctt ctgcagtcgg tcttcttgaa aagttcgttg
     6841 caaaatatgg cgaaagaaca ggagacttaa gcgatcgcta tcgtaaggct gagccagtgg
     6901 atgcagtacc ttctgttcca gacgctttat ggaagcttgc tcttgtatac caacaaatgg
     6961 gcgcaaatga taaggctaaa gccacagcag aaaaattatt gaaggtctat ggggattctc
     7021 aaatatattc tgataaagcc aaaaaattta ttgcaagcct atagtttttt tgaatcgtta
     7081 aaagatttgt tgccagctaa taatgaatag ctggcatttt tattttaaag aaatcattct
     7141 tgcaaagttt ataattaagt tttcgttgat tctttaaaaa ctttgtataa aaaaactcga
     7201 agtgatcttc gagttttttt attgtgttta attattattt catgaatttt gccgcttgta
     7261 caatcgcaaa aaccattcct ggtctaaagt attttccagc ggtatatttt agggcggtat
     7321 tatgaattaa cgagaataga gcggtctcat ctacttttgc tgtttgaatt aaagacttgt
     7381 aggtcttctt aaaagtagcg aggtctcttg taagcatagg agatacacga ctatcaggca
     7441 atagaatttt tgccgataat aggttcttat gtaacttcgc tgcactcgcc tttgaaaggt
     7501 taagattctt ttcgattgtt tcaggatgta gtctgcaact accgttaaac ccttcaggag
     7561 cggcaaaagg aatacgagct tcgttataaa gatttttcac catgctttca attaaatctt
     7621 ccgcttcgct ttttaagcca tgcatgagca ttgctgtaat caagctaaat tggataccaa
     7681 tccagacatc gtgagcttgg aaattaaatt catctaaagg cgagccatcg acatgaacca
     7741 aattagcagc accgatgaga gggctgtttg ccttatagtt catttcataa actttcttca
     7801 atgaacgacg tactttctgc acatctgtga taggggataa tccgagaagt ctcagataag
     7861 tatcggcaag catggagtct acaaaaatat catcattatc taagtgaatc tttttctgcc
     7921 aagatggagc aaaggcttta gggcattgtg cagtaatcca ttc
//
LOCUS       DAOOBB010000081         7958 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_10975_length_7958_cov_4.734911, whole genome shotgun sequence.
ACCESSION   DAOOBB010000081 DAOOBB010000000
VERSION     DAOOBB010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 7958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7958
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10975_length_7958_cov_4.734911"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="PLT31_05055"
     CDS             <1..832
                     /locus_tag="PLT31_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94542.1"
                     /translation="NDLLALRTSHRTFHSQTGGAMFGMISADASESYIVKNHGGLHLV
                     NYRSKQPVVWSDTVNWETNIIGQVTDSMVYGGDLWNEIVLWKIGEKPKKIKPKWSGNC
                     AFRLRKSSIPDFRVRPWRDGKFLLLGKTPTNQLRADTCQYAVLDTSTSEAVLSRFNSE
                     EEAWLSECLDIRYSNNQIICLKAKGLYQIDIVVNAELSDSKKINGNLEFTELWGNYIK
                     VDEPSFTDFILNLENLKFNENYSTIWIYTSPLNRYFVDSLGNEIWYTSEEFSHLESNR
                     "
     gene            829..1788
                     /locus_tag="PLT31_05060"
     CDS             829..1788
                     /locus_tag="PLT31_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94543.1"
                     /translation="MKKLFCILAIALFLISCSRTEEWWDSERTAGRIVGIVNDSLALQ
                     TSHRTFHSQTGGAMFGMISADASESYSIKGNGGLHLVNYRSRQPVVWGDTVNWETIIM
                     GQVTDSMVYGGDLENGIVLWKIGEKPRKIKPKWSGSCVGKLHRYSASPASRFQIRPWM
                     DEKLLVLGMGIMPANTIGGDTCQYGVLDTLTGIILLKRFNAEEAWLASCLDFHFYKNN
                     IICVQKNQLNNHQIDIWADGEKKDSAITQMQGIYTELLGKYVKIQESMFLDFILNIED
                     LKFDKNYSTIWIHADPVDRFFVDSLGNEIHYTSNDFSHLDTKP"
     gene            2150..3472
                     /locus_tag="PLT31_05065"
     CDS             2150..3472
                     /locus_tag="PLT31_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94544.1"
                     /translation="MATAFLKGRICFIFILLLTATGVIAAPSKNVENLFIFGIPSHNQ
                     VQKALQNSQIQKQGVLKKSAPRQDYSEKLDSIVYQYYDEEFIFDAYEKRKETFLYDSY
                     GNLITRIKSEYNDGEWEDVDKNDYLYDSNGNLVSKMSYSLSGDEWEADVKYEYSYDSN
                     NTLLFMTTTYWDYIEGEWEGVSIKYDYSYDAKGILISEIRSYMNNNQWENGGKTYYLY
                     DSIGNLISEINSKYNDGEWEEEEKIDYSYDSNGKLISEIDSEYNDGEWEDVIKGDYSH
                     DSKGNLVSLIWYRWNNEWLTSRKYEVEYDLSVSIENIAFGAASEFSSLGLMQMVNKPT
                     HITSNVDIDMFLYYSPFKTTSLTKKSVASRANASVSINKIQGNFMFTNHLNEPVELIV
                     HALDGKRMSTSFIQPGTNFVDMTGYSVGVYLVSCRINGKQIQSFRIAR"
     gene            3703..4320
                     /locus_tag="PLT31_05070"
     CDS             3703..4320
                     /locus_tag="PLT31_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94545.1"
                     /translation="MTFKETLAPLVCFSIHQVCNYFPEFEKTNLTRWKKSGLVVRLRQ
                     GWYSFPEYKKDVNFIRLAANKIYKPSYISLQYALAFYGMIPESVVQMTCVSSLKTASF
                     ENDWGQFSYQNLKPSLMFGYEQKKIENTLSNGQTFSIALPEKALLDFLYLNPYYKTCE
                     DFEALRLDDDFMREELNRDILNQFLKKIDSKALEGRVKKLLELYD"
     gene            4313..5098
                     /locus_tag="PLT31_05075"
     CDS             4313..5098
                     /locus_tag="PLT31_05075"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPW94546.1"
                     /translation="MISLDYIRNFFPKQIGENPAFDKQMLKEYLQLLILDFISSSVFS
                     TKIVFIGGTNLRLIKNIDRFSEDLDFDCKDLSSENFNILTDDVVRYLQNNGLNAVVKQ
                     KESDRITAFRRSIYFLELLFDLQLSGYKEERLLVKIEAQDQKIEYKSIAVDVNRCGFF
                     FPIRVPPNDILCAMKISALLSRRKGRDFYDTMFLLSQVKPNPAFLKASNGIDSLACLK
                     KALLQVLTEVDLNVKKKDFEHLLFNVHSSEKILRFKDFVNAEL"
     gene            complement(5180..6565)
                     /gene="argH"
                     /locus_tag="PLT31_05080"
     CDS             complement(5180..6565)
                     /gene="argH"
                     /locus_tag="PLT31_05080"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HPW94547.1"
                     /translation="MSSKSNEKKMWDGRFESGMAQSMIDLSFSLHFDAELIEEDIEGS
                     IAHGKGLVESGVLTPEEYKTITDGLITILSDYKSGKPLWNDGDEDIHMAVERVLTERI
                     GSLGKKIHTGRSRNDQVATDFKLYMRHRTQEIIHLITDLKKTILKTAEDHFGKLMPGY
                     THLQQAQPIYFSHYIMAFFFVLDRDEKRLKNFLALHNELPLGSGAMAGSAFPYQRNLV
                     AKALGFENPSPNSIDAVSHRDMALELTSSLSIIAATLSRYAEDFVNWSTSEFGFLTLH
                     DAFSSGSSMMPQKKNPDSMELIRGKAGRMIGNYTALFTLVKGAPLSYSRDLQEDKEPV
                     FDSVKTIKVLLRVMNEAILTATWNFENMHKKMLPALLATDLADLLVESGIPFRDAHHI
                     VGHLVGEAAKQKMEFTDLSNEIWEKAGVPNVEKIKNQLTFEFSVSRRNIEGGTGPDSV
                     KAQFEKAKKLL"
     gene            complement(6590..7477)
                     /locus_tag="PLT31_05085"
     CDS             complement(6590..7477)
                     /locus_tag="PLT31_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545099.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HPW94548.1"
                     /translation="MQYTPYRDFLAKTFPSYPKIRKLPLNGGMTCPNLDGTKSFSGCS
                     YCNNKSFSPVWDLAKTDIVTQLEKFLPPLKNKYPKAGVLAYFQPYTNTYAPASRLREI
                     FTPVLMHPDIAGISIGTRPDCLPDETVALLAEMNQVKPIIVEVGLQTSNDKTLVAINR
                     KHTFNEFKEAVERLHKAGLIVTTHLIVGLPHETLDDFKATAQAVKNLPIAAVKIHPLH
                     IVCGTALAKEYADAEFELLSFEAYTEAVAEMIKILGKEIAIERFSGESPDDLLIGPSW
                     AGERDRIIKEVEKLLSLKS"
     gene            7650..>7958
                     /locus_tag="PLT31_05090"
     CDS             7650..>7958
                     /locus_tag="PLT31_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94549.1"
                     /translation="MNADSTLAIGILVASIVVIFSYLVDIFSHRIRIPSVLLLMAFGM
                     GIKVFLDVTGIGAPDFTKMLPFLGTIGLILIVLEAAMHLHLTRDALEVMSKAAIAAAT
                     L"
BASE COUNT         2392 a         1350 c         1697 g         2519 t
ORIGIN      
        1 taatgatttg ttagccttac gaacatcaca tcgtacgttt cattcgcaga ctggtggcgc
       61 gatgtttggc atgatatcag cagatgcaag tgaaagttat attgtaaaaa atcatggtgg
      121 tctgcatctt gttaactata ggagcaagca acctgtggtc tggagcgata ctgtcaattg
      181 ggaaacaaac atcataggac aagtgactga ttcaatggtg tatggtggtg atttatggaa
      241 tgaaattgtt ctttggaaaa tcggtgagaa acccaaaaaa ataaaaccaa aatggagtgg
      301 aaactgtgca tttaggttac gcaaaagttc tattcccgat tttcgagtaa ggccttggcg
      361 tgatggtaaa ttcttgctac ttggaaaaac accgacaaat caacttcggg cggatacatg
      421 ccaatatgcc gtattagata cctcaactag tgaagcggta ttaagtcgat ttaacagcga
      481 agaggaagct tggctttctg aatgtttgga tattcgatat tcaaataatc agattatatg
      541 tttaaaagca aagggtttat atcagataga tattgttgta aacgctgaat tatccgatag
      601 taagaaaatt aatggaaatc tagaatttac cgaattatgg ggaaactaca taaaagtaga
      661 tgaaccatct ttcactgatt ttattttgaa tttggaaaac ttaaaattta atgaaaacta
      721 ttccactatt tggatttata caagtccgct aaatcgctat tttgttgact ctcttggcaa
      781 tgagatttgg tatactagtg aagaattttc ccacttggag agcaatcgat gaaaaagctg
      841 ttctgtatcc tcgctatagc tctttttttg atttcttgct cccgtacaga ggaatggtgg
      901 gacagtgagc gaacggcagg acggattgtg ggaatcgtca atgattcttt agcgttgcaa
      961 acatctcacc gcacttttca ttcgcagact ggtggtgcga tgtttggcat gatatctgcc
     1021 gatgcaagcg agagctatag cataaaaggg aatggtggac tgcatttggt gaactatagg
     1081 agcaggcaac cagtggtctg gggcgataca gtcaattggg aaacaatcat catgggacaa
     1141 gtaacggatt caatggtata tggaggcgat ttggaaaacg gaatcgttct atggaaaatc
     1201 ggtgagaagc cgagaaagat caagccgaag tggagcggta gctgtgtagg gaaactacat
     1261 cggtattctg cttctccagc ttctcgtttt cagattcgac cttggatgga tgagaaattg
     1321 ttggtgctgg gaatgggaat catgcctgca aatacaatag gtggcgatac ttgccagtat
     1381 ggtgtattag acacattgac tgggatcata cttctaaaac gctttaatgc ggaagaagct
     1441 tggcttgctt catgtttaga ttttcatttt tataaaaaca atattatttg tgttcaaaaa
     1501 aatcaattaa ataatcatca aatagatatt tgggctgatg gagaaaaaaa agatagtgca
     1561 ataacacaaa tgcaaggaat atataccgag ttgttgggaa aatatgtaaa aattcaagaa
     1621 agtatgtttc tagactttat tttgaatata gaggatttga aattcgataa aaactattcc
     1681 actatttgga ttcatgcaga tcccgtagat cgtttttttg ttgactctct tggcaatgaa
     1741 attcattata ccagcaacga tttttctcac ttggatacaa aaccatgaaa aaaatattct
     1801 tcacattggc tatctttgcg atagcttctc tcgcaagcaa ccaaccgatg gaagccatca
     1861 ccaattacaa tgtcatcatg gtgcatggag aagatatgaa taagctaatg cagtaaaaac
     1921 aaaataatat tgcttcttgt gaaaagcgtg ccataggcca tcagatcatt ttttattcat
     1981 gcgaagagca tacctttatt ttatatgaaa ggatttgcaa tacaaacgca tttatagctt
     2041 caaaagggct cgctcccatt gattagaaga ttaagtaaaa atatattgta aaaacgattc
     2101 atatatctat attataaata tggcgattaa aacagggcgg agatatcaaa tggcaactgc
     2161 ttttctaaag ggaagaatct gtttcatatt cattttatta ctcactgcaa caggtgttat
     2221 tgctgcacct tcaaagaatg tggagaacct ttttatattc ggcatcccta gccataacca
     2281 agtgcaaaaa gccctgcaga acagccaaat tcaaaaacaa ggtgtactaa aaaaatcagc
     2341 tccaagacag gactactctg aaaaactaga tagtatagta tatcaatact atgatgaaga
     2401 attcattttt gatgcctatg aaaaaagaaa agaaactttt ctttatgatt cttatggtaa
     2461 cctcattacc agaattaagt ctgaatataa tgatggagag tgggaggatg tagataaaaa
     2521 cgattatttg tatgactcta atggtaacct cgtttccaaa atgagctata gtttatctgg
     2581 tgacgaatgg gaagcagatg tgaaatatga atattcgtac gattcaaata acacactcct
     2641 tttcatgact actacttatt gggattatat cgaaggggaa tgggagggtg tgagtatcaa
     2701 atacgattat tcgtatgatg ctaaaggcat ccttatttct gaaattcgtt cttatatgaa
     2761 taataaccag tgggagaatg gaggtaaaac ctattattta tatgattcta ttggcaacct
     2821 catttcagaa attaactcta aatataatga tggagagtgg gaggaagaag aaaaaatcga
     2881 ttattcatat gattctaatg gtaaactcat ttcagaaatt gactctgaat ataatgatgg
     2941 agaatgggag gatgtaataa aaggcgatta ttcacatgac tctaaaggca atctcgtttc
     3001 tttgatctgg tatcgatgga ataatgagtg gctaacaagc cgaaaatatg aagtagagta
     3061 tgatctttcg gtttcaatag aaaacattgc ctttggagct gcctctgaat tttcatcttt
     3121 gggcctaatg caaatggtaa ataaaccaac ccatataaca agtaatgtgg acattgatat
     3181 gttcctatac tactcgcctt tcaaaaccac ttctcttaca aaaaaatcag tcgctagccg
     3241 agcaaatgcc tctgtatcca tcaataaaat acagggtaat tttatgttca ctaatcatct
     3301 caatgaacca gtggaactca tcgttcatgc gcttgatggc aagcgaatga gtacctcgtt
     3361 tatccagcct ggtacaaact tcgttgacat gactgggtat tcggtcggtg tgtatcttgt
     3421 ttcatgccgt atcaatggaa aacagattca gtcattccga atcgcacgat aaagcattta
     3481 ttactaccat gtagtgcttg tgcatggatc ggccccttcg agcagtgggt ttgaatgcga
     3541 atatataagg aataattagt tctaagcaaa atagatatga catccctatt ctttggatta
     3601 atgattatca gattacgaat aggtgatata atatgtaaat tttattgttt ttgtttctaa
     3661 aagtatgtat atttacatat aaatggaaac aaaatgaatt ttttgacttt taaagagact
     3721 ttggctccgt tggtgtgttt cagtattcat caggtttgca actatttccc agaatttgaa
     3781 aagacgaatt tgacgaggtg gaaaaagagt ggccttgtcg tgcggttgag gcaaggatgg
     3841 tattcttttc ccgagtataa aaaggatgtc aattttatac gtcttgcggc aaacaaaatt
     3901 tataagccgt cctatatttc tttgcaatat gcgttggctt tttatggcat gattcctgaa
     3961 tctgttgtgc agatgacttg tgtcagttcg ctaaagacgg catcgttcga aaatgattgg
     4021 gggcaatttt cataccagaa tttgaaacca tcgttgatgt ttggctacga gcaaaaaaag
     4081 attgaaaaca cactatctaa cgggcaaact ttttccattg cccttccaga aaaggctttg
     4141 ttggattttt tgtatttgaa tccatattac aaaacttgtg aggatttcga ggcgttgcga
     4201 ttggacgatg attttatgcg cgaagaatta aatcgggata tcttgaatca attcctaaaa
     4261 aagatagata gcaaggcgtt ggaaggaaga gtcaaaaaat tattggagct ttatgattag
     4321 tttggactat atccgaaatt tcttccctaa gcaaattggg gaaaatcctg cttttgacaa
     4381 gcaaatgctc aaggaatatt tgcaacttct aattttggat tttatttcga gttcagtctt
     4441 ttcaacaaag atcgtgttca ttggtggaac aaacttgcga ttgattaaaa atattgaccg
     4501 tttttcggaa gatttggatt tcgactgtaa agatttaagc tcagaaaatt ttaacattct
     4561 cacagatgat gttgtccgct atcttcaaaa caatggtttg aatgcggtgg taaaacagaa
     4621 agaaagcgat aggattacag cgtttcggcg aagtatctat ttccttgaat tgctattcga
     4681 tttgcaatta agcggttaca aggaagagcg ccttttggtt aagattgaag cgcaagatca
     4741 aaaaattgaa tataaatcga ttgcagtaga tgtaaatcgc tgtgggttct ttttcccaat
     4801 tagggttcct ccgaatgata ttttgtgtgc aatgaaaata tcggcgttgc tttcgcgacg
     4861 aaaggggcgt gatttttacg acacgatgtt tcttctttcc caagttaaac cgaatccagc
     4921 gtttttgaaa gcgtcgaacg gtattgattc tttagcttgt ttgaaaaaag ctttgttgca
     4981 ggtgcttacg gaagtcgatt tgaatgtaaa gaaaaaagat tttgaacatc tattgtttaa
     5041 cgttcattct agcgaaaaga tccttaggtt taaggatttt gtaaatgcag aactctaggt
     5101 cggtgtgtat cggcttgggt ttgctttttt ttgaaatgaa ttgtgaatag tgctttttaa
     5161 gcgccgatta ttctatgatc tataaaagct ttttggcttt ttcaaactgg gctttgacgg
     5221 agtctgggcc tgtgccgcct tcgatattac gacggctgac agaaaattca aaagtcaatt
     5281 gatttttaat tttctcaacg ttaggtacgc ctgctttttc ccagatttca tttgataaat
     5341 ctgtgaactc cattttctgt ttcgccgctt ctccgactaa atgccctacg atatgatgcg
     5401 catctctaaa aggaatcccg gattctacga gaagatcagc aaggtctgta gcgagcaatg
     5461 cggggagcat ttttttgtgc atgttttcaa aattccatgt ggccgtgaga atggcttcgt
     5521 tcattactct taagagaact ttgatcgtct tcacgctatc aaagacaggt tctttatctt
     5581 cttgtaagtc gcggctataa gaaaggggag cgcctttcac caaggtaaat agggcggtgt
     5641 agtttcctat catacggcca gctttgcctc gtatgagttc catgctatca gggttctttt
     5701 tctgaggcat catggaagag ccgctgctga aggcatcgtg cagggttaag aatccaaatt
     5761 cgctagtgct ccagttgaca aaatcttcag cgtaacggct gagtgtcgct gcaataatcg
     5821 aaagtgaaga ggttaattct aaggccatgt ctcgatggct taccgcatca atgctattgg
     5881 ggcttggatt ttcaaaacca agagctttgg cgactaagtt tctttggtac gggaacgccg
     5941 accctgccat agcaccactt ccaagaggta gctcattgtg gagggcaagg aaatttttta
     6001 aacgtttttc atcacgatct agaacaaaaa agaatgccat gatgtaatga ctaaaataaa
     6061 ttggttgggc ttgttgcaag tgcgtgtagc ctggcatcaa ttttccaaaa tgatcttcgg
     6121 ccgttttgag aatggttttc tttaagtctg tgataagatg aatgatttct tgtgtgcgat
     6181 ggcgcatgta caatttgaaa tcagtagcga cttggtcatt tcggctgcgc cctgtgtgga
     6241 tcttttttcc taggctcccg atgcgttcgg tgagaacacg ttctacggcc atgtgaatgt
     6301 cttcgtcgcc atcgttccaa agaggttttc ccgatttata atctgaaaga atagtgatta
     6361 agccatcggt aatggttttg tattcttctg gggtgagtac gccagattcg acaaggcctt
     6421 ttccatgggc aatggaccct tcaatgtctt cttcaataag ttcggcatca aaatgcaagc
     6481 taaaacttaa atcaatcatg ctttgagcca ttccgctttc aaaacggcca tcccacattt
     6541 ttttctcatt cgatttggaa gacataaaaa cctctagaac aaaactgttt tacgatttca
     6601 aagataataa cttttcaact tctttaataa tgcgatcgcg ttctccagcc caagaagggc
     6661 ctattaataa gtcatctggg ctctcgccgc tgaatctttc gatagcaatt tctttgccta
     6721 ggattttaat catttcggca acggcttccg tgtacgcttc aaaagagagt aattcaaatt
     6781 cggcgtcggc gtattctttt gctaaagcgg tgccacagac aatgtggagt ggatgaattt
     6841 ttacggcggc gatgggtaga tttttgactg cttgtgctgt ggccttaaaa tcatctaacg
     6901 tttcgtgagg taggcccact attaaatggg tggtgacaat caagcccgct ttgtgtaatc
     6961 gctccacagc ctctttaaat tcgttaaaag tgtgcttacg gttaatcgcg actagcgttt
     7021 tatcattaga ggtctggagc ccaacttcta caataatcgg tttcacttga ttcatctcag
     7081 caaggagcgc caccgtttca tctggtaaac agtctggcct tgtaccaata gagattcctg
     7141 caatatctgg atgcattaaa acgggtgtaa atatttctct taagcgagaa gctggggcat
     7201 acgtattcgt ataaggctga aagtaagcca gaacacctgc tttagggtat ttatttttta
     7261 gtggaggtaa gaatttttct aattgggtta caatatctgt tttcgcaaga tcccatacag
     7321 ggctaaacga tttgttattg caataagaac agccagaaaa gctcttcgtt ccatctaagt
     7381 tagggcatgt catcccgcca tttaagggca gctttcttat tttaggataa gaagggaagg
     7441 ttttggctaa aaaatctcga taaggagtat attgcataaa acaataataa aaaatgaatt
     7501 tttgattgac ttttagaaca ctttaaaaag gtgttttttc ttcttttgat aggtgcattt
     7561 tttatgatac ccaattttta aagttatttt tatgacatta taatctataa tgatttggct
     7621 cgcccactta aagctaaggg aagaatgtta tgaatgctga tagtactctc gcgataggta
     7681 tacttgttgc ttctattgtg gtgatttttt cttatttggt agatattttt tctcatcgta
     7741 tcagaatccc ttcggtattg ttgctgatgg cttttggtat ggggatcaaa gtatttttgg
     7801 atgtcacggg tataggcgct ccagatttca caaagatgct tccattttta gggacaatcg
     7861 gcttgattct aattgtgtta gaagcagcca tgcatttgca tttaacaaga gatgccttgg
     7921 aagtgatgtc taaagcagcg atagcggcag ccactctt
//
LOCUS       DAOOBB010000082         7788 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_11260_length_7788_cov_4.583215, whole genome shotgun sequence.
ACCESSION   DAOOBB010000082 DAOOBB010000000
VERSION     DAOOBB010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 7788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7788
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11260_length_7788_cov_4.583215"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1682
                     /locus_tag="PLT31_05095"
     CDS             <1..1682
                     /locus_tag="PLT31_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPW94550.1"
                     /translation="MTLQRTLISEALNIAGHQNYPAGTGHGYAATISGDIGSFHHNLL
                     AHNAGRNWSLGGGLDADGNYAGRLDIFNNVVYNWLNRATDGGAHEVNFTNNYYKEGPA
                     TTLHLMLSADLEGAGGGSQSYYYSGNVLQSASGSFTCDGSNNECGRRYTLSNGQVLNW
                     DVFVTKPFFPSYATVQTAKAAYKDVLSDVGASMPFFDNHDTRIIEETKKGSYTYTGSV
                     GKLKGIIDKESDVGGFESYPSTSWADDYDTDLDGLPDWWEVMYGYNPKSAAGDFSDAN
                     ADRKQDGYTELERFLEWMARPNYKIEDGETQVVDLSTFTKGYGSGTYVLTDIPSGVVA
                     SVSGSKMSVSLSASFGGVAYITYKFTDSVGDTFTRKIGVRGNAPATQEAAAIAKCGAG
                     SSKQSVVQGDSIVGFCYTWTGATTVSVSGFPSSLDINIDESAKKVTISGRVTDAVGEY
                     AFSVNTVGAATNATKTGLITVTDMTSLDQKNINPKINTIVKDGYVYLRGVPSDVQITV
                     TDISGKHLMVQKTLRNFNSEVALDLRNISNGFYIIQVKGKNLQKRIKISKK"
     gene            complement(1780..3102)
                     /locus_tag="PLT31_05100"
     CDS             complement(1780..3102)
                     /locus_tag="PLT31_05100"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPW94551.1"
                     /translation="MSKRIVCKFGGSSVAEANQFKKIKAIIESDSNRKIVVVSAPGKR
                     TSKETKLTDLLYSTYDLASKGLDFSTPWKLICDRYLEICADLNIAPKIKDDLDALEQK
                     MISSPETLTTDFLVSRGEFLCAKIMAEYLNATFVDTFPIICFDDKYRITTKSYSDIAS
                     ALKDESKVYVLPGFYGSNLHGEVKTFSRGGSDITGAILANAIEAEKYENWTDVSGMLM
                     ADPRIVESPMPIEYVSYREIRELAYSGASVLHDESIAPCRAKNIPINIRNTNKPEDPG
                     TIIGPTPKTIKNPITGVAGRKGFSMIYIEKTMMNKEIGFGRRVLAVLESEGLSYELCP
                     SAIDSMSIVVDSKALYAVQETVLEDITQQMRPDRIKIFPGISLIATVGHGMTDKIGVA
                     AKLFTALAEQSVNVRIIDQGSSQINIIIGVEESDTEKAINAIYKAFIS"
     gene            3281..4201
                     /locus_tag="PLT31_05105"
     CDS             3281..4201
                     /locus_tag="PLT31_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPW94552.1"
                     /translation="MQTFKRLLSIAAKSTIPVLIYGESGSGKEIAARLLHKQSERREG
                     PFVAVNCGALAKNLIENLLEGSKKGVYTGSLSDQKGLVRAADHGTLFLDEIGEMPIDL
                     QSRLLRIIQEKAVLPIGESHSIPVDFRLICATHRDLKKQVQDGFFREDLFFRLHVFPM
                     RIPSLRERKEDFRDLALEIWNEIKQSSLAPEYPDLELSLEELAILARFSWPGNVRQLK
                     NVLQRYYLLKPYGESLQTILDQEVSSFSDVREIQKVRSNAPKWDHIMEALNKHGHNKS
                     KAAAFLGVSRGCLCYQIKKHQVHCKSLEER"
     gene            complement(4158..5894)
                     /locus_tag="PLT31_05110"
     CDS             complement(4158..5894)
                     /locus_tag="PLT31_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HPW94553.1"
                     /translation="MIQKTLVSALFFSLVASSFSFSAMPLFNQLGFTPESEKIVVIPG
                     NDANPIEVRDVFGKKVLDLKVPYVSEWPASGEETQVYDLSSIKKEGTYRLFRNGEYLG
                     NPIVVSKNPYEEVAKASLKWFYYQRAGVALEYPYAGKWARAAGHPDDKVIVYGTDKVT
                     NKKASKTKPRIISSPKGWYDAGDYGKYIVNSTITVFTLLALYEHFPAYADTLHWNIPT
                     IYKNLPPLLDEVRWNLEWMLSAQDKDGGVFHKVTTLKFSGSVAPEKDTDARYAIIKNV
                     VASLDFAGVMAQASVVYKKFDKPFADKMLKAAEKAYAWAKANPKAMYVQPEDVQTGSY
                     APGNEDGKDEFRFAASELYNATKKDLYLKDLKNNPFNANGPWWGDLNFLAVYRVALSP
                     AVFGDSLSQAAKKILIEEANSLRAEGDSSAYRLPMHTWNWNWGSNSAVANNGIILLHA
                     YYLTKDKSYLDGAQQTLDYLLGKNPLEISYVTGFGYRSPKNPHHRPSESDFVDEPVPG
                     MLVGGPHLGKQDIGEENWKCKDYAGANKPALAYIDHQCSYATNEVAINWNAPFAYLAS
                     ALQAIYSGLGAF"
     gene            complement(6218..>7788)
                     /locus_tag="PLT31_05115"
     CDS             complement(6218..>7788)
                     /locus_tag="PLT31_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94554.1"
                     /translation="FTVSEYPRPSIAFANGSWQVISPDTSVIGEWANKMYPVYVRVLE
                     EVCTNCDNALSLASSNQNLIFMDALGNPISSVNLVGGHAVFYIFATQAVEDAAIQVSN
                     SVFANTLVWLDIDLALPPVPQLEYAEMHDRDGDGIADSLFLTYTKSLSGIDFPDSVLW
                     SFGDTTKNALNKNQLQGLLTGDRSFVLTSNTGFTKNVFTGMPKEPYFGLATTWFTFVP
                     DNSLEKILFDVSGQIADKVGPILLAASVSEKTGGITSLSLTFSEALQDGDEKLFATLF
                     EYKYWREGILKPSIMAPVSGAAAEKYRYEMLFMPKEGEELPAVGDSVRFTPGVATDLG
                     GAHPHLNNPWVRIVGDLSTKIQATSLIAVDPGATYPEGSPAVQPILVDKEMSIDDIIE
                     KYGVHGHVIDFDMSSLLQNLQAEGDSTATPESIRLEYETHYFSSLGHFINSKSGTILC
                     SDQVFEGNCLINPGNIFLAWNMRSNEGRLVGAGVYIARIEIKIKSKKRVVSRKAKDYM
                     WGIQRVKNGGKLLE"
BASE COUNT         2480 a         1489 c         1546 g         2273 t
ORIGIN      
        1 acatgactct ccagcgtact ctaatttcag aagcgttaaa tattgcgggc catcaaaatt
       61 accctgcagg aacagggcat ggttatgccg cgacaattag tggtgatatc ggtagtttcc
      121 atcacaacct tttagctcat aatgcggggc gcaattggag tttaggtggt ggattagatg
      181 ccgatggaaa ttacgctggc cgcttagata tttttaataa tgttgtttac aattggttga
      241 atcgtgctac agatggtggc gctcatgaag tcaattttac aaataactat tataaagaag
      301 gccctgccac aacgcttcat ctcatgctga gtgctgattt ggaaggcgct ggcggtggat
      361 cacaaagcta ttactattcg ggcaacgtat tacaatcagc aagtggaagt tttacttgtg
      421 atggatccaa taatgaatgt ggccgtcgtt atacactttc caatgggcaa gtcttaaatt
      481 gggatgtatt tgtcacaaag ccttttttcc catcgtatgc cacagtacaa acagcaaaag
      541 cagcctataa agatgtttta tcagatgtag gagcaagcat gccatttttt gataatcatg
      601 atacgcgcat cattgaagaa acaaaaaaag gcagctatac ctatacagga agtgtcggaa
      661 agttaaaagg aattattgat aaagaatcag acgttggtgg ctttgaaagt tatccaagca
      721 caagttgggc agatgattat gatactgact tagatgggct tccagattgg tgggaggtca
      781 tgtatggcta taatccaaaa tctgctgcgg gtgattttag cgatgctaat gccgatcgaa
      841 aacaagatgg ctatacagaa ttagaacgtt tcttagaatg gatggctcgt cctaattaca
      901 agatagaaga tggcgaaact caggttgtgg atttatccac gtttactaaa ggatacggaa
      961 gcggaacata cgtattaaca gatattccaa gcggagtcgt ggcgagtgtt agtggttcca
     1021 aaatgagtgt atccttaagc gcttcttttg gtggagtcgc ttatattacc tataaattta
     1081 ctgatagtgt aggagatact tttactcgta aaataggagt tcgtggtaat gcccctgcca
     1141 ctcaagaagc tgcagccatt gccaaatgtg gtgctggaag ctctaaacaa agcgtggttc
     1201 agggcgattc cattgttgga ttttgttaca catggacagg agcaaccaca gtgtctgtta
     1261 gtgggttccc ttcttcttta gatataaata ttgatgagtc cgcaaaaaaa gtaacgattt
     1321 ctgggcgtgt cacggatgca gtaggggaat atgcttttag cgtaaatact gttggagctg
     1381 cgacgaatgc tacaaaaacg ggacttatta cggtgactga tatgacttct cttgatcaaa
     1441 aaaatatcaa tcccaagata aacacaatcg taaaagatgg gtatgtctat ttaagaggag
     1501 ttccgagtga tgttcaaatc accgtaacag atattagtgg aaagcattta atggttcaaa
     1561 aaacattaag aaactttaat tcagaagtcg ctttggattt acgaaatata agtaacggct
     1621 tttatattat tcaggtaaaa ggaaagaacc ttcaaaagcg cataaaaatc tcaaaaaaat
     1681 aatgagacta atatgaatat ttaatccact agaaggattc tcttataaaa aaagcaacct
     1741 aaaaaaggtt gcttttgttg ttataaaaag aaaaaataat tacgaaataa atgctttata
     1801 aatcgcatta atcgcttttt ctgtatcgga ttcttcaaca ccaatgatga tgttgatttg
     1861 agaagaacct tggtcaataa tgcgaacgtt tacagactgt tctgcaagag cggtaaataa
     1921 tttggcagca acaccaatct tgtctgtcat gccatggccc actgtagcaa tcaagctaat
     1981 cccagggaat atcttgatgc gatctgggcg catttgttgc gtgatatctt caagaacagt
     2041 ttcttgcaca gcatagagcg ctttggaatc tacaacgata ctcatagagt caatggcact
     2101 tgggcaaagc tcataggaaa ggccttcgct ttcaagaaca gcaagaacac gacgaccaaa
     2161 accaatctct ttattcatca tcgttttttc aatgtagatc attgaaaagc cttttcttcc
     2221 agcgacacca gtaattgggt ttttgattgt tttaggagta ggcccaataa tggtacctgg
     2281 gtcttctggc ttatttgtat tacgaatatt gataggaatg ttttttgcac ggcaaggggc
     2341 aatcgattca tcgtgtaaaa cgcttgcacc agaataagca agctcgcgaa tttcacggta
     2401 gctaacatat tcaataggca taggactttc tacaattctt gggtctgcca tgagcattcc
     2461 agaaacatct gtccagtttt cgtatttttc agcttcaatg gcattggcaa gaatggcgcc
     2521 tgtgatatca gaaccgccac gagaaaaagt cttcacttct ccatggagat tagaaccata
     2581 aaagccaggc aatacataaa ccttgctttc gtcttttagg gcagaggcaa tgtcgctata
     2641 gctttttgtg gtgatacggt acttatcatc aaaacaaata attgggaaag tatctacaaa
     2701 ggtcgcattc aaatattcgg ccataatttt agcacagaga aattctccac gactcactag
     2761 aaaatcagta gttagagtct ctgggctaga aatcatcttt tgctcaagag catctaaatc
     2821 gtctttaatt ttaggggcaa tatttaagtc tgcacagatt tctaaataac gatcacagat
     2881 caatttccaa ggagttgaaa agtctaaccc tttagaagca agatcgtatg tgctgtaaag
     2941 taaatctgtt aatttagtct ctttcgatgt tcgtttacca ggagctgaaa ccactacgat
     3001 tttacgattc gaatcagatt cgataatcgc tttaattttt ttgaactggt tggcttctgc
     3061 aacagaacta ccgccaaatt tacatacgat tctttttgac attttgatct ttccttaaaa
     3121 gggaagtaag ttacaattgt aaataattac aatcgaatgt aaaaataata attgcagcgt
     3181 aaagaaaaag gggaataaat acgtttatat aagtgaagtt caaaaacgac gtttttctta
     3241 gatattcata aataaacgat aatctaaaca aaataaagca atgcaaacat ttaaaagact
     3301 actttctatc gctgcgaaat ccacaatacc tgtcttaatc tatggagaat caggttcagg
     3361 gaaagaaatt gcggctcgct tgttacacaa acaaagtgaa agaagagaag gcccttttgt
     3421 ggcagtgaat tgtggtgcct tagccaagaa tttaatagaa aaccttcttg agggttcaaa
     3481 aaaaggcgtt tacacaggtt ccctgagtga tcaaaaaggg ttagtgcggg cagccgatca
     3541 tgggactttg tttcttgatg aaataggcga aatgccaatc gatcttcaga gccgcttact
     3601 tcgaatcata caagaaaagg cggttcttcc aataggggag tctcatagca tccctgtcga
     3661 ttttaggtta atttgtgcaa cgcatcggga tctcaaaaag caagtgcaag acggcttctt
     3721 ccgtgaagac ttgtttttta ggctccatgt gtttcccatg agaattcctt ccttacgaga
     3781 acgaaaagaa gattttcgag acttagcttt agaaatttgg aacgaaatta agcaatcgtc
     3841 actggcgcca gagtatcctg atcttgaact atctttagaa gaattggcta ttttggcccg
     3901 tttttcttgg ccaggcaatg tccgccagct aaaaaatgta ttacagcgat actacctatt
     3961 aaagccttat ggagaatcat tacaaaccat tttagatcaa gaagtatcga gtttttcaga
     4021 tgtaagagaa attcaaaaag tacgttccaa tgccccaaaa tgggatcaca ttatggaggc
     4081 attaaataaa catgggcaca ataaaagtaa ggccgccgct tttctaggag ttagtcgcgg
     4141 ttgcctttgt tatcaaatta aaaagcacca agtccactgt aaatcgcttg aagagcgcta
     4201 gctaaatagg caaaaggagc gttccagttg atggccactt cattggtggc ataactgcat
     4261 tgatggtcaa tataagcaag agctggttta ttggcgcctg catagtcttt gcatttccaa
     4321 ttctcttcgc caatatcttg ttttcctaag tgaggcccac ctacgagcat cccaggaaca
     4381 ggttcatcta caaaatcaga ttcgcttggt ctatggtgcg gatttttagg gcttctataa
     4441 ccaaaaccag tcacataaga aatctccaaa ggatttttcc caagcagata atctaaagtc
     4501 tgttgagcac cgtctaagta gcttttgtct tttgttaagt agtaagcatg gagcaaaata
     4561 atcccgttat tagcgacagc actattggat ccccagttcc aattccaggt gtgcattggc
     4621 aaacgataag cagaagaatc gccttcggct cttaaagaat tagcttcctc tattaagatt
     4681 ttcttagctg cttgagaaag ggaatctcca aaaacagcag ggcttaaagc cacacgatac
     4741 accgcgagga aattcaaatc tccccaccag gggccattcg cattaaaagg attatttttc
     4801 aagtctttta aatacaaatc ttttttagtg gcgttgtaaa gttcagaggc tgcaaaacgg
     4861 aattcatctt tcccatcttc attacctggg gcatagcttc ctgtttgcac atcttcaggt
     4921 tgtacataca tggctttagg gtttgccttt gcccaagcat aagctttctc agctgcttta
     4981 agcatcttgt cggcaaaggg cttatcaaac tttttataca ccacagaagc ttgagccata
     5041 acaccagcaa aatctaaaga agctacgaca tttttaataa tcgcgtacct ggcgtctgta
     5101 tctttttcag gagccacaga gccagagaat tttaaagtag tgactttatg gaaaacgcct
     5161 ccatctttat cttgagcaga aagcatccac tctaaattcc agcgcacttc atctaataaa
     5221 ggaggaagat ttttataaat agtagggatg ttccaatgaa gcgtgtctgc ataggcaggg
     5281 aaatgttcat ataatgctaa aagagtaaat actgtgattg tggagttcac aatatatttt
     5341 ccataatcac cagcatcata ccagccttta ggggaactaa taatacgagg tttcgtttta
     5401 gaggcttttt tgtttgtcac tttatctgtc ccataaacaa tcaccttatc atctggatgg
     5461 cctgctgctc tggcccattt gcctgcataa gggtattcta aagcaacacc agcacgttga
     5521 taataaaacc atttcaagga agcttttgcg acttcttcat aaggattttt agaaacaaca
     5581 atggggtttc ctaaatattc accgttacgg aataatcggt aagttccctc tttttttata
     5641 gaggacaaat cataaacttg agtttcttca ccactagcag gccattcact cacataagga
     5701 actttaagat ccagcacttt tttcccaaaa acatctcgga cttcaatagg attggcgtca
     5761 ttcccaggaa tcactacaat tttttcagat tcaggagtaa aacctagttg attaaacaaa
     5821 ggcatcgcag aaaaagaaaa agaactagcg actaagctaa agaaaagagc agaaacaaga
     5881 gttttttgaa tcataaagcc tcatttatga gaaaaacgga atgccatttt aataaaaaat
     5941 agatgtaccc ttaaagaaaa tactttccta aaaaacaaaa agacgtatat aacgctcttt
     6001 tttgtattcc ctagtaaaca aaaaaggcca ttttgcaccc aatttcgatt aatcaaaggc
     6061 ttgcgcattg tacgcttgaa taaccatgcc cttataaaga taacgctaat gaatagtgaa
     6121 ttattagaat gattaatagg ggtttcaaat cattttttca ataaaaaagg tcgcctttat
     6181 tattggcgac ctgctttgat tttatttgaa taacatgtta ttctaaaagt ttgccaccat
     6241 tttttacgcg ttgaataccc cacatataat ctttcgcttt tctagaaact acacgctttt
     6301 tagatttgat ttttatttca attcttgcga tataaacgcc tgctcctact aaacgacctt
     6361 cattacttct catattccaa gcgaggaata tatttcctgg atttatgaga caattccctt
     6421 cgaatacttg atcggagcaa agaatagttc ccgattttga attgatgaaa tgtccaagag
     6481 aagagaaata gtgtgtttcg tattccaagc gaatggattc aggtgttgca gtagagtcgc
     6541 cttctgcttg taaattttgc aagaggctag acatatcaaa atcaatgacg tggccatgaa
     6601 caccatattt ttcaataata tcatctatgc tcatttcttt atcgacaaga ataggttgca
     6661 cagctggaga gccttctgga taagttgccc ctggatccac agcaataaga gaggtggctt
     6721 gaatcttagt agacaaatca cctacaatac gaacccatgg gttatttaga tgaggatgag
     6781 caccgcccaa atcagtggcg actcctggag taaagcgaac gctatcacct acagcaggga
     6841 gctcttcccc ttcttttggc ataaataaca tttcatagcg gtatttctcc gcagcggctc
     6901 cagaaacagg agccataatg gatggtttta aaatgccctc tctccaatat ttatattcaa
     6961 agagggttgc aaacaatttc tcgtctccat cttgaagggc ttcgctaaat gtgagtgata
     7021 aagaagtaat accaccagtc ttttcagaga cagaagccgc taaaagaatt gggccgacct
     7081 tatctgctat ttgaccgctt acatcaaata gaattttttc aagagagtta tcaggaacaa
     7141 aagtaaacca agtcgttgct aaaccaaagt atggttcttt tggcattcca gtaaagacgt
     7201 ttttagtgaa ccctgtattt gatgttaaaa caaaagagcg atcacccgtc aacagacctt
     7261 gaagctgatt tttattgagg gcgtttttcg ttgtatcccc aaagctccat aatacggaat
     7321 caggaaaatc tatgcctgaa agcgattttg tgtaagtaag gaataaacta tctgcaatac
     7381 cgtctccatc ccgatcatgc atttcagcat attctaattg tggaacagga ggtaaagcta
     7441 aatcaatatc aagccatacg agagtatttg caaaaaccga attagaaact tgaatggctg
     7501 catcttctac cgcttgtgtt gcaaatatat agaataccgc atgaccgccc actaaattca
     7561 cagaagaaat agggttacct aaagcatcca taaaaatcaa attctgattt gaagaagcta
     7621 aggataaggc gttgtcacaa ttggtacaga cttcttccaa aacacgtacg taaacagggt
     7681 acattttatt agcccattct ccaattacag aagtatctgg agaaatcact tgccaagatc
     7741 cattggcaaa agctatagat ggtcttgggt attcagaaac ggtaaacc
//
LOCUS       DAOOBB010000083         7774 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_11295_length_7774_cov_4.041715, whole genome shotgun sequence.
ACCESSION   DAOOBB010000083 DAOOBB010000000
VERSION     DAOOBB010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 7774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7774
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11295_length_7774_cov_4.041715"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="PLT31_05120"
     CDS             <1..648
                     /locus_tag="PLT31_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HPW94555.1"
                     /translation="RVMMELLNEPAGQDSKVWDKLKDELFWTIRKHAPKNTIVVGSNR
                     WNSAHEFKYLTPMEDDNAIYSFHTYTPVCFTHQKAAWIQDPFFHQERSWPGDYTPPTG
                     DEKVRLNYEYGRWDKARLQASLQNALDFRAKFNLPVSCNEFGVYVQTPRKYQIAWMRD
                     FLDILKEADVGFSYWNYKNLDFGLISKGESLHNSLPQYNNPERLDSELMEMLAKG"
     gene            882..1184
                     /locus_tag="PLT31_05125"
     CDS             882..1184
                     /locus_tag="PLT31_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94556.1"
                     /translation="MKAVAMLFALMFIFVACKNEDHKTSDNQKFQDVQESQIPLEAKK
                     ALNPLGVEEPELNAAPSKPKTEGAFDFSDCKEKHGTGTEQFRACVRAKYPNMKFVE"
     gene            1228..1659
                     /locus_tag="PLT31_05130"
     CDS             1228..1659
                     /locus_tag="PLT31_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94557.1"
                     /translation="MFFNTLLFADCIVYENRTYAETGAETYRKTCNEFGGGGSVFSSS
                     ISVSSANNTCDGGYADGAYVLRVSCNTCSSKKILDELKEHKEYCKEICKSSQMYCSNT
                     LNSWGSSLVGSLQSCGLEDHTLSGCSESSSSVSYKRKNEVF"
     gene            1767..2375
                     /locus_tag="PLT31_05135"
     CDS             1767..2375
                     /locus_tag="PLT31_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94558.1"
                     /translation="MICKIILYVLGVFTCLYAKVPADSSLIEEDPVFANFCFYKDTST
                     FTPSENGAINHIDSAFILTYYEMERDLSFTITFSSTTSSTIFGVKLPNLDMNKIGTNF
                     LQGMTGSRCIRATSYGRKHRGGTCSGKVEFTPYDHIDFFFSTKDSLYVKSLFDTETQL
                     VWKYNLSMGMQKQEVKGENIIYISGFCNEDQLKMIQKRKKKK"
     gene            complement(2676..3677)
                     /gene="gap"
                     /locus_tag="PLT31_05140"
     CDS             complement(2676..3677)
                     /gene="gap"
                     /locus_tag="PLT31_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPW94559.1"
                     /translation="MALKLGINGFGRIGRMVFRAAVQNFSKDIQVVGINDLLDADYLA
                     YMLKYDSVHGRFQGEVSVEGNFLVVNGNKIRLTAEMDPANLKWNEVGADVVVESTGFF
                     LDDASARKHITAGAKKVIMSAPSKDSTPMFVYGVNDSSYAGQDIISNASCTTNCLAPI
                     SKVLDEKFGIVRGLMTTVHAATATQKTVDGPSKKDWRGGRGILENIIPSSTGAAKAVG
                     KVLPQLNGKLTGMSMRVPTSDVSVVDLTVELKKETSYAEICAAMKAASENELKGVLGY
                     TEEAVVSTDFRDDARTSIFDAKAGIQLDSTFVKVVSWYDNEWGYSNKVCEMARVIAK"
     gene            3811..7575
                     /locus_tag="PLT31_05145"
     CDS             3811..7575
                     /locus_tag="PLT31_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HPW94560.1"
                     /translation="MKRSKSIFFEFFLFFSIFLIISCASSGAPLTKTRETGSQEQDYN
                     TSYYDEESTTAEPSRKTTGLIVRNGYQFELPSTDWEVVSDLNDEGAPLEFYNSKNGLR
                     GVVQSLFLESGEALQIMDRAQIEMQSYETLGKKASFSNVEGATKLGIMGAFWEIEGTR
                     SNQPYQACGFVAGAKQNVYMLSLSLSDSALPLNELKKEWDSLYKSFKIEESFIQETGP
                     EISPQVIKNHESKSLGYTWTTKDSLWHYWLSIARQNNDPDLVLTNKKEDLTLFVYGAT
                     ISPDEVTSQDLFKVLLLRLGVDPNNSTLSTSRERGGSASSYIQNFELTQYIGKYDFKY
                     KGRFFWENGRGILIAGWTQGALFPKYEKVMDRAINGFTFNKDFTKISDEKQEKFNAAV
                     LSQVGLLRLSENQPLVALSYFERANRLDPSEPLYLINCGFIYQLKELYGPGISYFSTQ
                     MDLVRKNGKLLSILGEMYEALFDYANARKFAELALQYTPNNPEYVINLSDALWGLGQR
                     NQSLLVVQRLYDKQPSSRLGVYLAKTYMGLDQYAEAVEVLYHSRNRFGTNIDLGLSLM
                     DALLFLKRYDEALAISEEVIQKDKNDTRIWTERGKTQFYLKNFRQAEKSLTKAVQLKS
                     DNEEAKSFLSATKAFLGKADNRTLQKMINPIEARSKNLKALINKNVAEKARNADFPAV
                     IHYNRELLKVEKGNPWVRTEEMLMQILDRRGSAIYQEFAFDFLPGFDRIFLNALEVYD
                     STMTLKHTATLQNAYITYATEIGSDNESQTAHFPLPPLEPGDFIYYQVSRTSLENKGI
                     IPYTDYMSSKDIPVAQTSFKIFADTSKFVTEEYGPLQRIDYPDAREWKIEEPVVIRKE
                     LYMPVYRDFGAGILITGKQEWKNVGEDYENMIRHQFKSAVSVREKAFEVKGSRLGQEA
                     VHAIIQFVRENIRYREVAFGGHSLIPQTAEVTLKERRGDCKDQSLLLKEMLDVIGVPS
                     HLVAIHLTEMGFEKLPTIQQFNHMILHIPAGDKYPEMWVDPTDKFGNNRPIPLDMEGK
                     VALIVNGEKSHVTTTPVLEDDQEHKVYIHHQLFIKANGESEFKDSLTLEGKFASFLRN
                     QFFGKDTKEQEKILEGLLSQGIPDVSISQIKTENATTFDKPFILVVVFTSNGYFGKSD
                     GGIKGRYPNIWERQFMRMPKVSKRHHPIRMPHETQFQSTFDIKTNAGDLEVKNIGKLT
                     REPEYISFEKKKDGIHWTTFALYADPNEYEKIREEWNYLLSETSPLLLVK"
     gene            complement(7577..>7774)
                     /gene="thiS"
                     /locus_tag="PLT31_05150"
     CDS             complement(7577..>7774)
                     /gene="thiS"
                     /locus_tag="PLT31_05150"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HPW94561.1"
                     /translation="QIYYNNTIVEVATTISLQTFLSDLGCDRLGVAAALNQQVIARTE
                     WSKTTLKEGDSVIVIEIAQGG"
BASE COUNT         2581 a         1451 c         1475 g         2267 t
ORIGIN      
        1 agggtaatga tggaactctt aaatgagccc gctggtcaag attcaaaagt ttgggataag
       61 ttaaaagatg aacttttctg gacgattcga aaacatgctc ccaaaaatac gattgtcgtc
      121 ggttctaatc gctggaatag tgctcatgaa tttaagtatt taaccccaat ggaagatgat
      181 aacgccatct acagttttca tacttatacc cctgtttgct ttacacatca aaaagcggct
      241 tggatccaag acccattctt tcatcaagaa cgttcttggc ctggggacta cactcctcca
      301 acaggcgatg aaaaggttag attaaattac gaatatggcc gctgggataa agcgcgctta
      361 caagcctctt tacaaaacgc ccttgatttt agagccaagt ttaatttacc tgtatcttgt
      421 aatgagtttg gggtttatgt tcaaacgcct cgtaaatacc agattgcatg gatgagagac
      481 tttttagata tcttaaaaga agccgatgtc gggtttagtt actggaacta taagaatttg
      541 gattttggct tgatttctaa gggagaaagc ctccataata gccttcctca gtacaataat
      601 ccagagcgtt tagattctga attaatggaa atgctcgcga agggataagt gataatccat
      661 cctataaaat agggataaag acttatttta ctgaggacgc ccttgtagtt gcgtttaatt
      721 ttcgcgtctt tgtattcctt cttcaatata aggtctcgtc cgtttccctt ttacaaaaga
      781 gctagataca ttgagaatta tgttagaata tccacttcta tttgcataaa ttccataatt
      841 tagctattct tgtccttgat tggattagat aggaggactc gatgaaagca gtagcaatgt
      901 tatttgcatt gatgtttatt tttgtggcat gtaaaaacga agaccataaa acgtcagata
      961 atcaaaaatt ccaagatgta caagaatctc agattccttt ggaagctaaa aaagccctaa
     1021 atccattggg agttgaagaa ccagaattga atgcggctcc ttctaagccc aaaacagaag
     1081 gagcattcga ctttagcgat tgtaaagaaa agcatggtac tggtactgaa cagtttcgcg
     1141 catgtgtaag ggcgaagtat cctaatatga agtttgtgga ataagaggaa cagaataatt
     1201 aaaaagatta tctttttaat ctgcgtgttg ttttttaaca cattactgtt tgcagattgc
     1261 attgtttatg aaaacagaac ttatgctgag actggagctg aaacatatcg caagacttgt
     1321 aatgagtttg gtggcggagg tagcgttttt tcgtcttcta tatccgttag ttctgcaaat
     1381 aacacttgtg atggcggtta tgctgatggt gcttatgttc tgagagtatc atgtaacact
     1441 tgtagttcaa agaaaattct tgacgaattg aaagagcata aggaatattg taaggaaata
     1501 tgcaaatcgt ctcaaatgta ttgctcgaac accttgaatt cttgggggag ttctttagtt
     1561 ggatccctac aatcctgtgg cttagaagat cacacacttt ctggttgttc tgaatctagt
     1621 tcatctgtca gctataaaag aaaaaatgaa gtcttttgaa aaacagttaa tagtggacac
     1681 tgaattgcga ttagataacg ctacaatccg ctatcataaa aagtacaaca cctatggata
     1741 ccccaaggag aaatatgttt gtccgaatga tttgtaaaat aattctatat gtattgggtg
     1801 tatttacgtg tttatacgca aaagttcctg cggatagttc tctgatagag gaagatcctg
     1861 tttttgctaa tttctgcttt tataaagata cgagtacatt cactccaagt gaaaacgggg
     1921 caatcaatca tattgactcc gcattcattt taacatatta tgaaatggaa agagatttgt
     1981 ctttcactat tactttctca agtacaacta gttcaacgat ctttggtgtg aagcttccaa
     2041 acttagatat gaataaaatt ggaacaaatt ttttacaagg gatgacagga agtcgttgta
     2101 taagagcaac ttcatatgga agaaaacatc gcggaggaac ctgttcggga aaagtagaat
     2161 tcactccgta tgaccatatt gattttttct tctcaacaaa ggattcgcta tacgtcaaat
     2221 cactattcga cacagaaaca caattggtgt ggaaatacaa tctaagtatg ggaatgcaaa
     2281 agcaagaagt taagggagag aatataattt acatctctgg cttttgtaac gaagatcagt
     2341 tgaaaatgat tcagaagcgg aagaagaaaa aataggttat actatgcaca aatgttctct
     2401 gttacaataa aacatactat tgtccaaaaa attagttgta caattttagt attactggct
     2461 ccgatatttg ctcaaaaaaa gaaactctct ggccaacggg ctacaattta tccatcctat
     2521 aaaataggga taaagactta ttttagatat acaatgcctt actttatatg ccttattttg
     2581 taaggcattt ttttatcaat agtttcaaag atttgtttta aacaaaaaaa tgctccgaat
     2641 aaattcggag catttttctt tcttttcaga aaaaattatt tagcgatcac gcgagccatt
     2701 tcgcaaactt tgttgctgta gccccattcg ttatcatacc atgaaacaac cttaacgaag
     2761 gtagaatcaa gttggatacc agctttagca tcaaagatag aagtacgtgc atcgtcacgg
     2821 aaatcagtag aaacaaccgc ttcttctgta taaccaagaa cacccttcaa ttcattttca
     2881 gaagcagcct tcatagcagc acaaatttct gcataggagg tttccttctt gagttctact
     2941 gttaagtcaa caacagaaac gtcagaagta ggaacgcgca tagacatacc agtaagcttg
     3001 ccgttcaatt gtggaagcac tttaccaaca gcctttgcag cacctgtgga agaaggaatg
     3061 atgttttcaa gaataccacg gccaccgcgc caatccttct tagaaggacc gtcaacagtt
     3121 ttttgagtcg ctgtagcagc atgaacagta gtcataagac cacgaacgat accaaacttt
     3181 tcgtcaagaa ccttagagat tggagccaag cagttggttg tgcaagaagc gttagaaata
     3241 atgtcttgac cagcatagga gctgtcgttt acaccatata cgaacattgg tgtagagtcc
     3301 ttagaaggag cagacataat cactttctta gcaccagcgg tgatgtgctt acgagcagaa
     3361 gcatcatcta agaagaaacc agtggattca acaacaacgt cagcaccgac ttcgttccac
     3421 tttaagttag ctggatccat ttcagctgtt aaacgaattt tattgccatt aacaacaagg
     3481 aagttacctt cgacagacac ttcaccttgg aagcgaccat gaacggaatc atactttaac
     3541 atgtatgcga gataatcagc atcgagaaga tcgttaatac caacgacctg gatgtctttt
     3601 gagaaatttt gaacagcagc acggaaaacc atgcgaccaa tacgaccaaa accattaata
     3661 ccaagtttaa gagccatttg aggaatcctc atttgttgat tgtgaatagg cgcagctctc
     3721 acaatcgaga gccaacggtt gcttgcaaat ttatttaaaa ttaaggatat tgcgaagaca
     3781 ttttattctt tttctgcact aggaaccact atgaaacgat caaaatccat tttttttgaa
     3841 tttttcctct ttttttccat atttcttata atttcctgcg cttcttcagg ggctcctctt
     3901 acaaaaacaa gagaaacagg ttctcaagag caagattaca acacttccta ttacgatgaa
     3961 gaatcgacaa cagctgaacc atccagaaaa accacagggt taatcgttag aaatggatac
     4021 caatttgaac taccaagtac agactgggaa gtggtaagtg atctaaacga cgaaggagca
     4081 ccgctcgaat tttacaatag caaaaacgga ctgcgtggcg tagtacaatc cctcttctta
     4141 gaatctggtg aagcccttca aataatggac cgtgctcaaa ttgaaatgca aagttatgaa
     4201 accctaggga aaaaagcctc tttttcaaat gtagaaggag cgactaaatt aggcatcatg
     4261 ggagcgtttt gggaaattga aggcacgcgt tctaaccaac cataccaagc gtgtggtttt
     4321 gttgctggag cgaagcagaa cgtttatatg ctctcgcttt cactttccga ttccgcatta
     4381 cctctaaacg aattaaaaaa agaatgggat tctctttata aatctttcaa aatagaggaa
     4441 tccttcattc aagaaacggg tccagaaatt tcgccacaag tcattaagaa ccacgagtcg
     4501 aagtcccttg gttatacttg gactacgaaa gattctctat ggcattattg gctaagtatt
     4561 gcaaggcaaa ataatgatcc tgatttagtt ctcacgaaca aaaaagaaga ccttacttta
     4621 tttgtatatg gagccactat ttctcctgac gaagtcactt ctcaagattt gtttaaggtt
     4681 ctgctcttac gcctaggcgt tgaccctaac aactccacgc tttctacatc tcgcgaacgt
     4741 ggtggttctg cctcttctta cattcaaaat tttgaactca cccagtatat tggaaaatac
     4801 gattttaaat acaaaggacg ctttttctgg gaaaacggtc gaggcatttt aattgcaggc
     4861 tggacacaag gcgctttatt tccaaaatat gaaaaagtaa tggatcgagc aattaacggc
     4921 tttactttca ataaagactt caccaaaatt tctgatgaaa aacaagaaaa atttaatgcc
     4981 gctgttctta gtcaagtggg tttactacga ctatctgaaa accaaccttt ggtggcctta
     5041 agttactttg aacgagccaa tcgcttagat cccagtgagc ctctttattt aattaactgt
     5101 ggctttatat atcaattaaa agagctttat ggcccaggca tcagctactt ttctacccaa
     5161 atggatctcg ttcgaaaaaa cggaaagttg ctttctattc ttggtgaaat gtacgaagcg
     5221 ctttttgact acgccaacgc gagaaaattc gctgaacttg ctttacaata cacccccaat
     5281 aatccagaat acgtaatcaa cttaagcgat gccctctggg gattagggca acggaatcaa
     5341 tcattactag tggtccaaag actttacgac aaacagccct cctctagact tggagtctac
     5401 cttgctaaaa catatatggg attagatcaa tacgccgaag cagtcgaagt actttatcat
     5461 tctagaaatc gttttggtac caatatcgat ttaggccttt ctttaatgga cgctcttcta
     5521 ttcttaaaac gttatgatga agctctcgcg attagcgaag aagtgattca aaaagacaaa
     5581 aatgacactc gcatttggac agaacgcgga aaaacacaat tttatcttaa aaattttaga
     5641 caagctgaaa aaagcttaac taaagcagtt caactaaaaa gtgataacga agaagcaaaa
     5701 agtttccttt ctgcaaccaa agccttctta ggtaaagctg acaatagaac attacaaaag
     5761 atgattaatc ccattgaagc tcgttcaaaa aatttaaaag ctcttattaa taaaaacgtc
     5821 gccgaaaaag cgagaaatgc tgatttccct gccgttatcc actacaacag agaactttta
     5881 aaagtagaaa agggaaatcc atgggttaga acagaagaaa tgcttatgca gattttagat
     5941 cgcagaggaa gtgccatcta ccaagaattc gcatttgatt tcttacctgg atttgatcgg
     6001 atattcttga atgcattaga agtatatgat tcaacaatga ccttaaaaca tacagctact
     6061 ttacaaaacg cctatatcac ttatgccaca gaaattggta gcgacaacga atcacaaaca
     6121 gcgcattttc cactccctcc attagaacca ggtgatttta tttattatca ggtttctaga
     6181 acgagtcttg aaaacaaagg aattattcct tacaccgatt acatgtccag taaagatatc
     6241 ccagtagctc aaacttcatt taaaattttt gccgatacct cgaaatttgt cactgaagaa
     6301 tatggtcctc ttcagcgtat agattaccct gacgctagag aatggaaaat agaagagcct
     6361 gtcgtcattc gtaaggaact ttatatgcct gtctatagag attttggtgc gggtattctt
     6421 atcactggta agcaagaatg gaaaaatgta ggcgaagatt atgaaaacat gattcgtcat
     6481 caatttaaga gtgctgtctc cgttcgcgaa aaagcatttg aagtcaaagg aagccgttta
     6541 ggacaagaag ctgtacacgc gatcattcaa tttgtacgcg aaaatatccg ctatcgtgaa
     6601 gtcgcctttg gagggcattc tttaattcca caaaccgctg aagtaacatt aaaagaacgt
     6661 cgtggtgatt gtaaagatca aagcttactt ttaaaagaaa tgctagatgt cattggtgtt
     6721 ccaagtcatt tagttgccat ccatttaaca gaaatgggct ttgaaaaatt accaactata
     6781 caacaattta atcatatgat tttacacatc cccgcaggag ataaataccc agaaatgtgg
     6841 gtagatccaa cggataaatt tggtaataac cgtcctattc ctctggacat ggaaggaaaa
     6901 gtagcgttaa tagtgaatgg tgaaaaaagt catgtaacaa cgacacctgt tcttgaagat
     6961 gatcaggagc ataaagttta tattcatcat caattattta tcaaagctaa tggagaaagc
     7021 gaatttaagg attctttaac attagaaggt aaatttgctt cttttttaag aaatcaattc
     7081 tttggaaaag atactaaaga acaagaaaaa attcttgaag gcctattatc tcaagggatt
     7141 ccagatgttt cgatttcaca aatcaaaact gaaaatgcaa ctacctttga caaacctttt
     7201 atcttagtgg tagtctttac aagtaatggc tattttggaa aatctgatgg tggaattaaa
     7261 ggacgttatc ccaatatttg ggaaaggcag tttatgcgta tgcctaaagt cagtaaaagg
     7321 caccatccaa ttagaatgcc gcacgagact caatttcaaa gtacgtttga cataaagaca
     7381 aacgctggag atcttgaagt aaagaatata ggcaagctca caagagagcc tgaatacatt
     7441 tcgtttgaga aaaagaaaga tggcattcat tggaccacat ttgctctcta tgcagaccct
     7501 aatgaatacg aaaaaataag agaagaatgg aattatctct tatcagaaac gtctccacta
     7561 cttctagtaa aataatttag ccaccttgcg ctatttcaat aacaataaca gaatcaccct
     7621 ctttgagggt ggtttttgac cattctgtac gtgcaattac ttgctgattt agtgcagccg
     7681 ccactccaag gcgatcacat cctaaatcgg ataaaaatgt ttgaagtgaa atagttgtgg
     7741 ctacttcgac gatagtatta ttataatata tttg
//
LOCUS       DAOOBB010000084         7691 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_11438_length_7691_cov_4.238214, whole genome shotgun sequence.
ACCESSION   DAOOBB010000084 DAOOBB010000000
VERSION     DAOOBB010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 7691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7691
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11438_length_7691_cov_4.238214"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..754)
                     /locus_tag="PLT31_05155"
     CDS             complement(110..754)
                     /locus_tag="PLT31_05155"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HPW94562.1"
                     /translation="MKNLKIRLETESDYHHVEELTREAFWNLYFPGCDEHYLCHILRD
                     HKDFIKELDYVAEIDGRVIGSIMYTHSFLLSDDGEKVPTVSFGPIGVHPDYQRKGIGT
                     KLIEKTVALLKEMNVPAIIIFGDPHNYCKHGFKNGLDYQVSDMNGEYPLALLVLELQP
                     GFFGTKKWKVQQSEVFQFNPEAVHDFDKQFPEKEKKYQYSQEFFKMNIRAFLKG"
     gene            1232..2680
                     /locus_tag="PLT31_05160"
     CDS             1232..2680
                     /locus_tag="PLT31_05160"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HPW94563.1"
                     /translation="MKIKIESKESGKAIAHALFFVKKSIQFSSVLSEKATEQAETVLN
                     GMEEGPFEDLEFLELDGVPTFFVNAAKERGLTSLDHLRMAAYRLAKQAKKKQISCVSL
                     MLADAAPEQLKSIIHGLYYAEYEFDRYKTKKQKSFSVVYEIVVQDQAKEFSKIANDVA
                     IEQASISMARDWINTPGSDLTPSVFVSEAEKIAKETGLKIKVRDIKALQKEGYNGLVT
                     VGKGSQSLPYMVTLEYKPAKASKDLHLALVGKGLTFDTGGISLKPATGMWEMCCDMSG
                     AAAVLGAIRTIALLKLPIHVSAVLCLAENRIGSKAVLPGDIFTAKNGKTVMVDNTDAE
                     GRLALSDGLAEAGLLKATHIIDLATLTGAMVRALGYAVTGFFSNDEKLASKILEAGQT
                     CCEKFWPMPLEEEYADGLKGKFADLQNTGSDAGAISAALFLKEFVPENTSWSHWDIAG
                     TAFVTKPWKYTSHGATGIGVQTLVELARQLGE"
     gene            2685..4487
                     /gene="guaA"
                     /locus_tag="PLT31_05165"
     CDS             2685..4487
                     /gene="guaA"
                     /locus_tag="PLT31_05165"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HPW94564.1"
                     /translation="MKNVDTIAVLDFGGQYAHLIANRIRRLGVFTEIHSPTASVKELE
                     GVKGIVYSGGPSSVYEKDAPAFNPEILSIPVPKLGICYGHQLIATQLGGEVTPGKVKE
                     YGIAALEIKEQNHPLLEGIPQKSPMWMSHGDMVSTTPEGYKVIATTKDCETAAVAYDE
                     KQIYGIQFHPEVTHSQFGSKLLSNFIKICNCTGSWNMKSYLPLITERIKNQVQDKNVF
                     LLVSGGVDSTVAFVLLNKILGPSRVQGLHIDNGMMRLDESQKVLEFLDAHGMKNLIVN
                     DASEDFLQALKGITAPEEKRKRIGATFLQVKDREMSRLKLDPSQWMIAQGTIYPDTIE
                     SGGTKNADLIKTHHNRVKEVLDLMESGLLLEPLADLYKDEVRLLGEELGIPHNLVWRH
                     PFPGPGLGVRLLCTNGTHALPKKEDVDGLIPYLKEHGVEGYLLPIQSVGVQGDGRTYA
                     QPFLITSKNLDWKGCELFSTELANRFKAINRIVKEIGADSAEEPVLATQFPTRAAFDS
                     LRKFDHICTEFLQEHDLYEKIWQMPVVQTPLQINKKPCIVMRPVNSSEAMTANFAEID
                     FNLLEKLWLSLKEAGAGSLWYDVTHKPPGTIEWE"
     gene            complement(4614..5429)
                     /locus_tag="PLT31_05170"
     CDS             complement(4614..5429)
                     /locus_tag="PLT31_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94565.1"
                     /translation="MRKFLFLILAAVSLSFAAYDLFPVPQNKGANFRLNTNLNFGSVD
                     YSSETQYFTRLILDMRFYYKRIEVSMMGLGVENDAVNGNGITFANNMFFGAKYQLFST
                     FAFIFDLNYKEVYFYESDAEPGRSFDWKLGAQFSKKINQLLVFGSEIGFGYPSEFELE
                     GKDEKIIITPGKFLYLKGEVDYSLTRKIILFAAVDFDIKMTDSEYSFNPYGGRGNYDA
                     YDKYGAGEVLFQLNVGISYKFSKQFSIEEDNFIRFGALRSSSFGIAVILKYNL"
     gene            complement(5439..5861)
                     /locus_tag="PLT31_05175"
     CDS             complement(5439..5861)
                     /locus_tag="PLT31_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94566.1"
                     /translation="MFTSCATSNSSYHKARSVGLNANEKFQVYGAYKSNIVGTLTWAN
                     DDHCNENAFINYVRLQNKAQNVIEIIMNETCTDQNTKCKCSYSGIGVNYTAINAEEAT
                     VWNNAISSKNENITTPQKSKSLFSISGGAESEDSQQHR"
     gene            complement(6185..>7691)
                     /locus_tag="PLT31_05180"
     CDS             complement(6185..>7691)
                     /locus_tag="PLT31_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HPW94567.1"
                     /translation="QQNIDEVVKIIRGSVDTDSAKASLMERFGLSDIQAQSIVDMRLK
                     QLTNLEIDKIEKEYQELLVTIADLEDILAKRERQLEIILNRLDEINDKFGDDRRTSIE
                     DAAEDFDYEDLITEEEQVITLSREGYIRRLPIDTFKAQNRGGKGIIGAGLKEEDFVEQ
                     IFTASTHSYLLIFTNKGRVHWTKVYRLPEGTRNGKGRPIVNFIELSEGEKVSAIVPVR
                     KFGGYFCLVFATKKGVVNKMDLTLFSRPRKAGVNAITLDEGDELVKVKLVGVDNRSQE
                     DAPEIEGEDLIEETEVIDSETELDEGVVEEGRPIANDLLMIATKSGQAITFPISSIRA
                     MGRGTHGVRGIQLAEGDEVISLLWLKPNNKILTISENGYGKRSEPSSYRITRRGGKGV
                     KNLNVTEKTGEAVFVDSVADDYDLIITSRDGQVIRISVDSIRVTGRVSQGVKAITLRD
                     QDMVQDATALPSVEDIEQDSADAKETFDHIQGETVEDETEVSDSSSESSED"
BASE COUNT         2378 a         1556 c         1378 g         2379 t
ORIGIN      
        1 ctttttttat gataaatagt atttacaagt ttttcgtgtt cgattaataa tcagctcatt
       61 ttttttcgaa attcacttat caattatcta tggccattaa taattatcat taccccttca
      121 aaaaagctcg aatattcatt ttgaaaaatt cttggctgta ttgatatttc ttttcttttt
      181 cagggaattg tttatcaaaa tcatgcacgg cttctggatt aaactgaaat acttcgcttt
      241 gttgcacttt ccatttttta gtgccaaaaa agccaggctg aagttctaaa acaagaaggg
      301 ctaatgggta ttccccattc atatcgctca cttgataatc cagcccattt ttaaaaccgt
      361 gtttacagta gttatgagga tcgccaaaga tgataatcgc aggcacattc atttctttga
      421 gtaacgcgac cgttttctct attaattttg tacctatccc ctttctttgg taatcaggat
      481 gcacgcctat tgggccaaag gaaaccgttg ggactttttc gccatcgtca ctcaataaaa
      541 aagaatgcgt gtacatgata gaaccaatta cccgaccatc tatttcagcg acgtaatcaa
      601 gctcttttat aaaatcttta tggtctctta aaatgtgaca taaataatgt tcatcgcacc
      661 cagggaaata caaattccaa aaggcctcac gggtcaattc ttcgacatga tggtaatcag
      721 actctgtttc tagacgtatt ttcaagtttt tcatgtactt tgccccgaat tcttttattt
      781 agtagcaaat ataatcgatc tttcttaaaa ataagacgct ttttttaaga aacaagcctg
      841 tattgaatcg aacaccctta ttaattcgat cgactttatt gaatcgcact tatatgccaa
      901 aaatgacacc agcaagattc aaacattctg gcacccctat atccaagggc tcctagcaag
      961 gtttggaaaa gatcatggca tcaaccaatg ttttggaaaa aacgaaaagt ccttttttga
     1021 atatagccga gaagtgtttc aatcagtaat cgccgaaaaa tggcttaaaa attctggaaa
     1081 taatcgaaca taaatcagtc agcatttttt tgccaccata ttacatgtat aaatccatca
     1141 ccattttcga tagcgtatta tatgcttaca agagccttag aggggcttta tatgctttta
     1201 ataatgatgt tttttattat ctttacgcat tatgaaaatt aaaatcgaat ccaaagaatc
     1261 aggcaaggct attgcccatg ctttattttt tgtaaaaaaa tccattcaat tctcttctgt
     1321 tctttcagag aaagcaacgg aacaagcaga aacagtctta aatggaatgg aagaaggtcc
     1381 ttttgaagac ctcgaatttt tagagcttga tggtgtacct actttttttg taaacgctgc
     1441 taaggaacgt ggtttaacct ctcttgacca tcttcgcatg gccgcctacc gactcgctaa
     1501 gcaagccaag aaaaaacaaa tttcatgcgt gagcttgatg ttagctgacg ccgctccaga
     1561 gcagttgaag tcgattattc atggtttgta ttatgcggaa tatgaatttg atcgttataa
     1621 aacaaaaaaa caaaaatcat ttagtgtcgt ctatgaaatt gtagttcaag accaagcgaa
     1681 agaattttct aaaatcgcaa acgatgtcgc tattgaacaa gcctctattt ctatggcccg
     1741 cgactggatt aatactccag gttctgattt aacgccttcc gtttttgttt ctgaagcaga
     1801 aaaaatcgcg aaagaaacag gattaaaaat caaagttcgt gacattaaag ctcttcaaaa
     1861 agaaggctac aatggtttag tgactgtagg taaagggagc caatctttac cttacatggt
     1921 gactctagaa tataagcctg cgaaggcctc caaagatttg cacctcgctc ttgttggaaa
     1981 aggcttgacc tttgatactg gtggaatcag cttaaaacca gccacaggga tgtgggaaat
     2041 gtgttgtgat atgtctggag ccgctgctgt tttgggtgcc attcgtacca ttgctctttt
     2101 gaaacttcca attcatgtaa gtgctgttct ttgccttgca gaaaatagaa ttggttctaa
     2161 agcagttctt cctggtgata ttttcaccgc taaaaatgga aagactgtga tggtcgataa
     2221 tacagacgct gaaggtcgct tagctctttc agatggtctt gcagaggctg gcctcttgaa
     2281 agccactcat attattgatt tggctacttt aacaggagcc atggttcgtg ctcttggtta
     2341 tgccgttact gggttcttca gcaatgatga aaaactagct tcaaaaattt tagaagcagg
     2401 tcaaacttgt tgtgaaaaat tttggccaat gcctcttgaa gaagaatacg cggacggttt
     2461 aaaaggtaaa tttgccgact tgcaaaatac tggttccgat gctggtgcta tttctgccgc
     2521 tcttttctta aaagaatttg ttccagaaaa cacatcatgg tctcattggg atattgctgg
     2581 cactgctttt gtcacaaagc cttggaaata tacttctcat ggggccacag gaataggagt
     2641 gcaaacttta gtggaactcg cacgacaatt aggagaataa ttctttgaaa aacgtagata
     2701 ctattgccgt tcttgatttt ggtggtcaat atgcccatct tattgctaat cgtattcgtc
     2761 ggttaggtgt ttttactgaa atccactccc ccacagcttc ggtcaaagaa ctggaaggcg
     2821 ttaaagggat tgtgtatagt ggtgggccat caagcgtgta cgaaaaagac gctcctgctt
     2881 ttaatccaga aattttatcg attcctgtgc ctaaattagg catctgttac gggcatcaac
     2941 tgatagcgac tcaattgggt ggcgaagtga ctcctgggaa agtcaaggaa tatggcattg
     3001 ccgctcttga aattaaagaa caaaaccatc ctcttttaga aggaattcct caaaagtccc
     3061 ctatgtggat gagccatggc gatatggttt ctacaacccc tgagggttat aaagtcatcg
     3121 ccacaactaa agattgtgaa acggccgctg tcgcctacga tgaaaagcaa atttacggta
     3181 ttcaatttca tccagaagta actcatagtc aatttggttc caagcttctc tccaatttca
     3241 ttaagatttg taattgtacc ggctcttgga acatgaaaag ctacttgcct ttgattacag
     3301 aacgcattaa aaatcaagta caagacaaaa atgtgtttct cttagtctct ggcggtgtgg
     3361 atagcactgt tgcttttgtt ctcttaaata agattttagg gccttcacga gtgcaagggc
     3421 ttcatatcga taacgggatg atgcgccttg atgaatctca aaaggtgctt gagtttttag
     3481 atgcccatgg catgaaaaac ctaatcgtca acgatgctag cgaagacttt ttacaagccc
     3541 tcaaaggcat taccgcccca gaagaaaagc gtaaacgcat tggggctacg ttcttacaag
     3601 tcaaagaccg tgaaatgagt cgcttaaaac tagacccatc tcaatggatg attgctcaag
     3661 gcactattta tccagatacc atcgagagcg gcggcacaaa aaatgcagat ctaattaaaa
     3721 cacatcataa tcgcgttaaa gaagttcttg atttaatgga aagcgggctt ttattagaac
     3781 cattagccga tctttataaa gatgaagtta ggcttcttgg tgaagaactt ggtattccgc
     3841 acaatttagt ttggcgtcac ccttttccag gcccaggtct cggtgttcgt ttactttgca
     3901 ccaatggcac gcatgcattg cctaaaaaag aagacgtaga cggtttaatc ccctacttaa
     3961 aagaacatgg ggttgagggc tatttactgc caatccagag cgtaggcgtt caaggggatg
     4021 gccgtactta tgcacaacca ttcttaatca cctccaagaa tcttgattgg aaagggtgcg
     4081 aactctttag tacagaactc gccaatcgct ttaaggcgat caatcgtatt gtaaaagaaa
     4141 tcggagccga ttctgcagaa gagcctgttt tagcaacaca gttccctact cgcgccgctt
     4201 ttgactcttt acgaaaattc gatcacattt gcaccgaatt cttacaagag cacgatttgt
     4261 acgaaaagat ttggcaaatg cctgtggtac aaacaccgct tcaaattaat aagaagcctt
     4321 gcattgtgat gcgccctgtg aatagttctg aggccatgac agcgaatttc gccgagattg
     4381 attttaacct cctcgaaaag ctttggctct cgctcaaaga agctggcgct ggatccttgt
     4441 ggtacgatgt tacgcataaa ccaccaggaa caatcgagtg ggaataattg cccttaggat
     4501 aaaaaaatgc cgctagagac aatctgcggc tttttttgtg aaacgagaat aaaaatcatt
     4561 ttcaaaaata catcgcattt atgaacttca caaaagcgat aataaaatct atatcataaa
     4621 ttatatttca atatgactgc gattccgaaa ctcgaactac gcaaagctcc aaatcgaata
     4681 aagttatctt cttcaatgct gaattgttta gaaaatttgt aactaatgcc cacattcagc
     4741 tgaaacaaca cttcgcctgc tccatattta tcatacgcat catagttacc cctacctcca
     4801 tatgggttaa aagaatactc gctatcggtc attttaatat cgaaatcaac agcagcaaag
     4861 agaattatct ttcgagtaag agaataatcc acttcccctt tcaaatagag aaatttgcct
     4921 ggagtaatta ttattttttc gtcttttcct tccaattcaa attcagatgg atatccaaac
     4981 cctatttcag aaccaaatac caatagttga tttattttct tggaaaactg cgcccctaat
     5041 ttccaatcga aagacctccc aggctctgcg tcagattcgt aaaaataaac ttctttataa
     5101 ttcaagtcaa atatgaatgc aaaagtagaa aacagttggt atttagcacc aaaaaacata
     5161 ttgttagcaa aagtgatacc atttccattt actgcgtcat tttcaacgcc tagtcccatc
     5221 atggagactt caatgcgctt atagtaaaaa cgcatatcta gtataagcct agtaaaatac
     5281 tgcgtctcgc tagaataatc aacagaacca aagtttaagt tagtattcaa gcgaaaattt
     5341 gcacccttat tttgtggtac agggaacaaa tcgtaagcag caaacgaaag cgacaccgca
     5401 gcaagtataa gaaataaaaa ctttcgcatt tctattcctt atctatgttg ttgagaatct
     5461 tcgctttccg ccccacctga gatagagaat aaggacttac ttttttgagg agttgtaata
     5521 ttctcgttct tagaggagat agcgttattc catacagtcg cttcttctgc attgatggct
     5581 gtatagttta cgccgattcc tgaatagcta catttgcatt ttgtattttg atctgtacaa
     5641 gtttcgttca tgataatctc aatcacattc tgcgctttat tttgtaagcg aacataattg
     5701 ataaaagcgt tttcattgca atgatcatcg ttagcccaag ttaatgtacc tacgatatta
     5761 gacttataag caccataaac ttgaaatttt tcgtttgcat ttaagccaac agaacgtgct
     5821 ttatgataac tagaatttga agtagcacag cttgtaaaca aagtggctaa ggcaagaatg
     5881 gcaaatattt tttcataaca atccctttta aaaaatattg caccaaattt attaataaat
     5941 catcattcaa caagtcacga actggactat acaacagttt tttttaataa aaaaaggcgc
     6001 cataaagtca taatatgcgg tttttgaacg aaattctctg ttttttaaaa ttcaaaaacg
     6061 caaaaactga aattgacgcc aaaagttatt tttttgatac gccttaaata agtaaagaac
     6121 atcacataaa taaaaaagcc ctgaaagaat attctttcag agctctaatt tttaaggatt
     6181 ctttttagtc ttcagaggat tctgaagatg aatcagatac ttctgtttca tcttcaaccg
     6241 tttctccctg aatatgatca aatgtttctt tagcgtcagc agaatcttgt tcaatatctt
     6301 caacacttgg aagtgctgta gcatcttgaa ccatatcttg atctcttaag gtaattgctt
     6361 tcacgccttg agaaacacgg cctgtgacac gaatactatc cacagagatt cggattactt
     6421 gaccatcgcg actggtgata attaaatcat agtcatcagc aacgctatcg acaaaaacag
     6481 cttctcctgt cttttcggtg acgtttaagt ttttaacgcc ttttccacca cgacgagtaa
     6541 tacgataaga agaaggttct gaacgtttgc catagccatt ttctgaaata gtgagaatct
     6601 tattattagg ctttaaccag agaagagaaa tcacttcatc cccttcggcc aactgaatac
     6661 cacgaacacc gtgtgtgcca cgtcccatgg ctcttatgct tgaaattggg aaagtaatcg
     6721 cttggccaga ctttgttgca atcatcaata gatcatttgc aataggacga ccttcttcaa
     6781 ctacaccttc atcaagttca gtttcagagt cgataacttc agtttcttca atcaaatcct
     6841 ccccttcaat ttctggagca tcttcttgag atctgttatc tacgcccact aattttactt
     6901 tgacaagttc atcaccttca tccaaagtaa tcgcattgac tccagcctta cgtggacgag
     6961 agaatagagt taaatccatc ttatttacaa cacctttttt ggttgcaaaa acaagacaga
     7021 aataacctcc aaacttacgt actggtacga ttgccgaaac tttttcgcct tcgctaagtt
     7081 caataaagtt aacgattgga cgacccttac catttcttgt gccttctggc aaacgataga
     7141 cttttgtcca atgcactcgg cctttattag taaatatgag caaataacta tgggtacttg
     7201 cagtaaagat ttgttctaca aagtcttctt ctttaagacc tgcaccaata atacccttac
     7261 cacctctatt ttgagcctta aacgtatcaa taggaagacg gcgaatataa ccttctctac
     7321 ttaaagtaat cacttgttct tcttcggtga ttaaatcttc atagtcaaaa tcttcagcgg
     7381 catcttcaat agaagtacga cgatcatcgc caaacttgtc attaatttca tctaagcgat
     7441 ttaaaataat ttcaagttga cgttctcttt tagcaagaat atcttctaaa tcagcaatcg
     7501 taacaagcaa ttcttgatat tctttttcaa ttttatcgat ttcaaggttg gttaactgct
     7561 tgagcctcat atctacaata gattgagctt gaatatcaga taaaccaaac cgttccatca
     7621 aagaagcttt agctgaatct gtatcaacag agcctctgat aatttttacg acttcatcaa
     7681 tattttgttg a
//
LOCUS       DAOOBB010000085         7358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_12078_length_7358_cov_4.564699, whole genome shotgun sequence.
ACCESSION   DAOOBB010000085 DAOOBB010000000
VERSION     DAOOBB010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 7358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7358
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12078_length_7358_cov_4.564699"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="PLT31_05185"
     CDS             <1..828
                     /locus_tag="PLT31_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014544952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HPW94568.1"
                     /translation="VGDTVYGILFLILTLVAQAKMPKAPKAAFHFLILMSCTTIIWGF
                     LNNSFLGFSPAALDLAQKSFVPEFVKKLALWIREASNVQYLCFILAVIHLTIAHLWNF
                     IIRVKSRSTTAIAQIGWLFSTWFMFFLASNMVLKKEMPEFAMPLFITGLVLIVLFMVP
                     PKDLKKEWISFPMLIFDVINNFVDIISYIRLFAVGMAGVAIAQAFNMMLSPLFGSAVG
                     IAVSVIALFFVHCLNIALGIMGVAVHALRLNTLEFSNHLGLQWSGSEFTPFVKQKMN"
     gene            859..1317
                     /locus_tag="PLT31_05190"
     CDS             859..1317
                     /locus_tag="PLT31_05190"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732433.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the K subunit is a
                     nonenzymatic component which binds the dimeric form by
                     interacting with the G and E subunits; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HPW94569.1"
                     /translation="MEQTTMVSLGSIGAMASLGIAAIGSALGTGIAGAAAIGVWKKAY
                     AQGKSALFTLLVFVGVPISQTIYGMLLMNSILASLKATGGANWGAALGAGIFGGLGMA
                     CSAWFQGRAAANACDSLGETGKGMANYLMVCGVVETVALFVMVFSMLNLG"
     gene            1396..2598
                     /locus_tag="PLT31_05195"
     CDS             1396..2598
                     /locus_tag="PLT31_05195"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HPW94570.1"
                     /translation="MKKKEIRNQIYSVGVLNPTLRTFDVFMRTEYGTTYNVYIVKGEN
                     KTALIESVNRERFDEFFENLKEVINPAEIDYIILNHTEPDHSGSLAKILEYAPNAVVV
                     ATATALRFLKNIVNQDFPSLTATEDLVLDLGKRSLHFKIAPNLHWPDSMFTYLPEEKM
                     IFTCDFLGAHYCESPLELSKIYKPNLYEGAFRFYYDCIFSPFKKFVLAGLNKMDGLDI
                     EFLAPSHGPALDSKAAIEKAKSLYREWSTEKDPGHTATIVYVSAYGYTKKLALEIAEE
                     LKKEDFKVSLFDLSSDDFAKAAEAALSSKLLFIGSSTINRNALPTIWNFLTGLDAIAL
                     AGKPVGVFGSYGWSGEAVPALKAYLTQMRCKVVGEGFKAVFQPSETELAQVKEYAKTA
                     IESIKKPV"
     gene            2695..4137
                     /gene="purB"
                     /locus_tag="PLT31_05200"
     CDS             2695..4137
                     /gene="purB"
                     /locus_tag="PLT31_05200"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014544966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HPW94571.1"
                     /translation="MRDQFESPLIQRYASKEMSFLFSPQFKFQTWRKLWIILAESEKE
                     LGLPITEEQIEELKAHDTDINFDVAEAEEKRRRHDVMSHVYAYGVQCPKAKGIIHLGA
                     TSAFVGDNTDLIQMQKGLILVRRRLMRVIDKLSQFALKNKDLPQLGATHFQAAQLTTV
                     GKRACLWIQDLLIDLEEIDFLIEVLPFRGVKGTTGTQASFMELFNGDEAKIVELDRLV
                     TTRAGFKRCLTITGQTYTRKWDNRVSQALSSIAQSLHKLATDMRLMQGLKEVEEPFES
                     TQIGSSAMAYKRNPMRSERICSLARFVISLVESTAFTQATQWFERTLDDSANKRLAIP
                     EAFLAMDAMLIIAENVSNGMVVYPKVIEKRIMAELPFMATENIIMEGVKKGGDRQELH
                     EEIRVMSMEAGKVVKQEGKDNDLLERIIKNPTFNALGITEDKLKEILDLKKFIGRAPG
                     QVVRFVEEEVKPMLNKYNDWQNLDAGELKV"
     gene            complement(4111..4782)
                     /locus_tag="PLT31_05205"
     CDS             complement(4111..4782)
                     /locus_tag="PLT31_05205"
                     /inference="COORDINATES: protein motif:HMM:NF016873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HPW94572.1"
                     /translation="MINLKAMPLIVTCEHASNKVPSYLKKYFLEEESILKTHHAYDIG
                     VEAIFNQVIKKWNPFYSCKGKYSRLCIDLNRSLRHPKLFSDLSKAAPIEIKKRLLQEW
                     SSYRDPIISAIRKQRLSHKNKDVFHLSVHSFTPKMNNIERNADIGILYDPARSLEKEV
                     ALELKKRILQQNPLLKIKMNYPYLGKSDGFATSLRKEFSKGYVGIELEINQKIIDLDF
                     KLPCI"
     gene            complement(4775..6028)
                     /locus_tag="PLT31_05210"
     CDS             complement(4775..6028)
                     /locus_tag="PLT31_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014544968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HPW94573.1"
                     /translation="MTVLKKKQWNIFERFGIELEYMIVNKDTLQVIPRADIALGKDKK
                     GNPLSEIERGDVGLSNELVSHVVEFKCASPISSFSLWSKNLQKEINRVNKELAKINAM
                     LLPSATHPFMNPLEEMVIWPYESNEIYTTYDRIFNCKGHGWANLQSTHLNISFNGDAQ
                     FETLHAAIRILLPLIPAIAASSPYLDSKFTQFKDARLETYRHNQERIPSIAGQIIPEP
                     VFSYAEYDRVIFSTIKKDIAPYDPENLLNHFFLNSRGAIARFDRGAIEIRLVDIQECP
                     AADIAIAELQIAVLKTIASQKWSPIERQKELSTTSLANILNKTIKVAENAIIDDKSYL
                     LALGIEESSIKASDVWKHLYNHCKKFITPASQKILEKILVRGTLSTALLKKIGERPSR
                     DRLVFEYAKLAECLAKNTLYEIDHD"
     gene            complement(6025..>7358)
                     /locus_tag="PLT31_05215"
     CDS             complement(6025..>7358)
                     /locus_tag="PLT31_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014544969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RimK family protein"
                     /protein_id="HPW94574.1"
                     /translation="VSLLAEARGHKVIPNVKSIRDFKAPAVVKIVSDEIDSLIQKSLR
                     KLSGSEFVLSIYFGQNVSSQYLELSQELYRIFQAPLLRAKFIFKQKWYIQSIRPICFN
                     EIPDNHLSMVDQFAKEYFEKTRFFSAKADNYLYDLAILINPDEKEPPSNEKAIQKFIS
                     AAEETGFRVELITQKDYHRVGEFDALFIRETTNVNHATYSFARRAQSEGIAVIDDPDS
                     ILRCSNKVYLQELMNVGHIPAPKTIITHSENRNVLAKELGFPLILKSPDSSFSQGVIK
                     VKNKEELEEELDRMFTKSDLLIAQEFTPTDFDWRIGVLDGKPLYACKYYMAKDHWQIY
                     NWKSEDKNEICGNFDCLPIESVPHGIVKAALRVSAMIGNGLYGVDLKEVNGKPVVIEV
                     NDNPNIDAGIEDQVAKKKIYLAIMRSLRHRIEDRLNAAQLKIQHHERDFFI"
BASE COUNT         2171 a         1275 c         1465 g         2447 t
ORIGIN      
        1 gtgggcgata ccgtttatgg tattttattc cttattttaa ctttggtggc gcaagcaaaa
       61 atgcctaagg ctccaaaagc agccttccat tttttaattt taatgagctg tacgacaatc
      121 atctggggct ttctaaataa cagcttcctt ggtttttctc cagcagcttt agatttagct
      181 caaaaatcat ttgtcccaga atttgttaaa aaattagctt tatggattag agaagcaagt
      241 aacgtacaat acttatgttt catattagct gttattcatt taaccattgc tcatctatgg
      301 aactttatta ttcgtgtgaa aagtagaagt actaccgcta ttgctcaaat cggctggctc
      361 ttttccactt ggtttatgtt cttcttagca agcaacatgg tcttgaaaaa agaaatgcct
      421 gaatttgcaa tgcctctttt tattacaggt cttgtgttga tcgttttgtt tatggttcca
      481 ccaaaagact taaagaaaga atggatttct tttccaatgc ttatattcga tgtaatcaat
      541 aactttgtcg atatcattag ttatatccgt ctctttgctg ttggtatggc tggtgtggct
      601 atcgctcaag cctttaatat gatgttatct ccattatttg gctctgctgt tggaatcgct
      661 gtttcagtaa ttgctttatt ctttgtgcat tgtctaaata tagctttagg tattatgggt
      721 gtcgctgttc atgcacttcg tttaaatacg cttgaatttt caaatcatct tggtttacaa
      781 tggagtgggt ctgaatttac tccttttgta aaacaaaaaa tgaactaatt ttaatcaaca
      841 aacatagagg aaaatgttat ggaacaaaca acaatggtta gtctaggaag cataggcgca
      901 atggcctctc tcggtattgc ggcaataggt tccgccttgg gtactggtat agctggtgca
      961 gctgcaatcg gtgtttggaa aaaagcatat gctcaaggga aaagcgctct ttttacatta
     1021 ctcgtttttg tcggtgttcc tatttctcaa accatttacg gaatgctatt gatgaactcg
     1081 attcttgctt cactaaaagc cactggtggt gctaactggg gagctgctct tggtgcaggt
     1141 attttcggtg gattaggtat ggcttgttct gcttggttcc aaggtcgtgc agcagcaaat
     1201 gcttgtgatt ctttgggcga aacaggcaag ggtatggcaa actatttgat ggtttgcggt
     1261 gtcgttgaaa cggtggctct tttcgtcatg gttttctcca tgctaaatct cggctaaaag
     1321 aaactttttc ttatgaaacc ccgctttttt aagtggggtt ttttatttta tagtaaaaac
     1381 ggaggagtat tttagatgaa gaaaaaagaa attagaaatc aaatttattc tgtaggtgta
     1441 ttgaacccaa cgcttcgtac ttttgacgtc tttatgcgta cagaatacgg gacaacttat
     1501 aatgtttata tagtgaaagg cgaaaataaa acggccttaa ttgaatccgt taatagagaa
     1561 cgttttgatg agttttttga aaacttaaaa gaagtgatta atccagcgga aatcgattac
     1621 atcatcttaa accatacaga accagaccat tcgggatctt tagcaaaaat tcttgagtat
     1681 gcgccaaatg ccgttgtggt tgcgacagcg actgctttac gtttcttaaa aaatattgtg
     1741 aatcaagatt tcccttcttt aactgctaca gaagatttag tcttagattt agggaagcgt
     1801 tccttgcatt ttaagattgc tcctaatttg cattggcctg attcaatgtt cacgtatctt
     1861 ccagaagaga agatgatttt tacttgtgat tttttaggcg ctcattattg tgaaagccct
     1921 ttagaacttt ctaagattta taagccaaat ctttatgaag gggcattccg tttttactac
     1981 gattgtattt ttagcccatt caaaaagttt gttttagctg gcctaaataa aatggatggc
     2041 ttagatatag aatttttagc tcctagccat ggcccagcgt tagattcaaa agcggcgatt
     2101 gaaaaagcaa agtctttgta cagagaatgg agtacagaaa aagaccctgg gcatacagct
     2161 acgattgttt atgtttctgc ctatggctac actaaaaagc tcgcattaga aattgcagaa
     2221 gagttaaaaa aagaggactt taaggtatct ttgtttgatt tatcctctga tgattttgct
     2281 aaggcggcag aggctgcttt aagttcaaaa ttactattta tcggctcttc tacaattaat
     2341 cgtaatgctc tgcctacgat atggaatttc ttaacaggct tggatgcgat tgctcttgca
     2401 gggaagcctg ttggcgtatt tggttcttat ggttggagcg gcgaagcggt gcctgctctt
     2461 aaagcctatt taactcaaat gcgttgcaaa gtcgttggcg aaggctttaa ggctgtattc
     2521 cagccttctg aaacggagct tgctcaagtc aaagagtacg ctaaaacagc aatcgaatcg
     2581 attaaaaaac ctgtttaatt ttaacttttt ctatattttg attcatcatt tttcaggcgt
     2641 tctataaaaa agtctttgta gaccagaccg ccttgttttt taaaaggatt ttttatgcgc
     2701 gaccaatttg agagtccctt aattcaaaga tatgcttcca aagaaatgag ctttctattt
     2761 agtcctcaat tcaagtttca aacttggaga aaattgtgga ttatccttgc tgaaagcgag
     2821 aaagaattag gccttcctat cacagaagaa caaattgaag aattaaaagc gcatgatacg
     2881 gatatcaatt ttgatgtcgc cgaggccgaa gaaaaacgcc gccgccatga tgtgatgagc
     2941 catgtgtatg cttatggcgt tcaatgccca aaagccaaag ggattattca tttaggggct
     3001 acgagtgctt ttgtaggcga taacacggat ttaattcaaa tgcaaaaggg cttgatttta
     3061 gtacgccgcc gtttgatgcg tgtgattgat aagctttctc aatttgcatt aaaaaacaaa
     3121 gatttacctc aattaggtgc gactcatttt caagccgctc aattgactac tgttggcaaa
     3181 cgcgcttgtt tatggattca agatttattg attgaccttg aagaaattga tttcttaatt
     3241 gaagtgttgc catttagagg cgttaaaggg actacaggaa cacaagcttc ctttatggaa
     3301 ctattcaacg gagacgaagc aaaaattgtt gaattagatc gtcttgtgac aactcgtgct
     3361 ggttttaagc gttgcctcac gattacgggc cagacttata cccgtaaatg ggataatcgc
     3421 gtgagccaag ctctttcttc tattgctcag agcttacata aactagcgac cgatatgcgt
     3481 ttgatgcagg gcttaaaaga agtcgaagaa ccttttgaat ccactcaaat aggctcgagt
     3541 gcgatggctt ataaacgtaa ccccatgaga agtgaacgta tttgttctct cgctcgtttt
     3601 gtgatttctt tagtggaaag cactgctttt acccaagcca ctcaatggtt tgaacgtaca
     3661 ttagatgata gtgctaataa acgtcttgca attccagaag cattccttgc gatggatgcg
     3721 atgttgatta tcgctgaaaa cgtttcaaac ggtatggttg tttatcctaa agtcattgaa
     3781 aaacgtatca tggctgaact tccatttatg gctaccgaaa acatcattat ggaaggtgta
     3841 aaaaaaggtg gcgatcgtca agaattgcat gaagaaattc gtgtgatgag tatggaagct
     3901 ggaaaagtgg tgaaacaaga aggaaaagat aacgatcttt tagaacgtat catcaaaaac
     3961 ccaacattca atgctcttgg gattactgaa gacaaactaa aagaaatttt agatcttaaa
     4021 aaattcattg gtcgtgcccc tggtcaagta gtgcgttttg ttgaagaaga agtaaaacca
     4081 atgcttaaca agtacaatga ttggcaaaac ttagatgcag gggagcttaa agtctaaatc
     4141 aattattttt tgattgattt caagctcaat tccaacatac cctttagaaa attcttttcg
     4201 aaggcttgtg gcgaaaccat cgcttttgcc taaataaggg taattcattt taatttttaa
     4261 taaaggattc tgttgaagga tccttttttt taattccagt gccacttctt tttcaagaga
     4321 tcgcgcgggg tcgtataaaa taccgatgtc cgcatttctt tcgatgttgt tcattttagg
     4381 cgtaaaagag tgaacactta gatgaaatac atctttattt ttatggctta atcgttgctt
     4441 acgaatagca gaaataatag ggtctcgata agaactccat tcttgaagaa gtcgcttttt
     4501 tatttcaata ggagcggctt tgcttaagtc agaaaatagt ttgggatggc gtaaactacg
     4561 gtttaagtcg atacaaagcc tggaatattt tcctttacaa gaataaaaag gattccattt
     4621 tttgataacc tgattaaaaa tggcttctac gcctatatca taagcatgat gagtctttaa
     4681 tatggattct tcttctaaaa aatacttctt taagtacgag ggcactttat tagaagcatg
     4741 ttcacaagtg acaattagtg gcattgcttt tagattaatc atgatctatt tcataaagcg
     4801 tgtttttggc aagacattct gctaatttag cgtattcgaa aacaagacga tcacgacttg
     4861 ggcgttctcc aattttcttt aaaagagctg tagataaagt accacgaact agtatttttt
     4921 ctaaaatctt ttgcgatgca ggagtgataa attttttgca gtgattgtat aaatgtttcc
     4981 aaacatcgga tgcttttata gaagattctt ctattcctaa cgctaataaa taagatttat
     5041 cgtcaatgat tgcattttca gccactttta tcgtcttatt taagatattt gcaagtgaag
     5101 tagtgcttag ctctttttgc ctttctatgg gcgaccattt ttgagaggca atggttttca
     5161 aaacggcaat ttgtagttca gcaattgcaa tatcggcggc agggcattct tgaatatcaa
     5221 ctaaacgaat ttcaattgcc cctctgtcaa aacgggcaat cgctccacga ctattcaaaa
     5281 agaaatgatt taatagattt tctggatcat agggggcaat gtctttttta atagtagaaa
     5341 aaatcactcg atcgtattct gcataagaaa atacgggctc tggaataatt tgcccagcaa
     5401 tagaaggaat tctttcttga ttgtgcctgt aggtttcaag cctagcgtct ttaaattgag
     5461 tgaatttact gtctaaataa gggcttgatg cagcaatcgc tggaattaaa ggtaaaagaa
     5521 tacgaatggc ggcatgaagc gtttcaaatt gagcatcacc attgaaagat atatttaggt
     5581 gtgtggactg taaattcgcc cagccatgcc ctttacaatt aaaaatgcga tcataagtgg
     5641 tgtatatctc attggattcg taaggccaaa tcaccatttc ttctaatggg ttcataaatg
     5701 ggtgagttgc cgaaggcaag agcatcgcat tgattttggc gagttcttta tttactcgat
     5761 taatttcttt ttgaagattt ttactccata aagaaaatga agatataggg gaagcgcatt
     5821 taaactccac gacgtgtgaa actaattcat tagagagccc cacatctcct ctttcaattt
     5881 cagaaagagg attccctttt ttgtcttttc caagcgctat atcggctctt ggaatgacct
     5941 gaagggtatc tttattgacg atcatgtatt ccagttcaat accaaatcgt tcaaagatgt
     6001 tccattgctt tttttttaga actgtcatat gaaaaaatca cgctcatggt gttggatttt
     6061 taattgtgct gcatttaaac ggtcttcaat tcgatgtctt aaagagcgca tgatggctaa
     6121 gtagattttc tttttagcga cttggtcttc aatacctgcg tcaatatttg gattatcgtt
     6181 gacttcgatt acgacaggct ttccattgac ttcttttaga tcgactccat ataaaccatt
     6241 accgatcatg gccgatactc gtaaagcggc tttcacaatt ccatgaggaa cggattctat
     6301 aggtaagcag tcaaaattac cacagatttc gtttttatct tcagatttcc agttgtagat
     6361 ttgccaatgg tctttagcca tatagtattt acaagcataa agaggttttc cgtctaaaac
     6421 accaattctc cagtcaaaat cagttggtgt aaattcttga gcaattaata aatcactctt
     6481 tgtaaacatt cggtctagtt cttcttcaag ttcctctttg ttttttacct tgatgactcc
     6541 ttgagaaaaa ctcgagtctg gggattttaa aattaaagga aatcctagtt cttttgctaa
     6601 aacatttctg ttttctgaat gagtgataat cgtttttggg gcaggaatat gaccgacatt
     6661 catcaattct tgaagataca ctttgtttga acaacgtaaa atactgtctg gatcatcaat
     6721 tacggcaatg ccttcggatt gagctcgccg tgcaaaacta taagtcgcat gatttacatt
     6781 ggtggtttca cgaataaata aggcatcaaa ttcaccgacg cgatgatagt ctttttgagt
     6841 aatgagttca actcggaatc cagtttcttc tgcagcggaa atgaactttt gaatggcttt
     6901 ctcgttacta ggaggctctt tttcatctgg gttaatgaga atcgctaagt catacaaata
     6961 attgtccgct tttgcagaaa agaaacgagt tttttcaaag tattctttag caaattggtc
     7021 gaccatcgaa agatgattat ctggaatttc attaaaacag ataggacgaa tgctttgtat
     7081 ataccatttt tgtttaaaaa taaactttgc tcgtaggagt ggagcttgga aaattcgata
     7141 caattcttga gataactcta aatattgaga gcttacattt tgaccaaaat aaatagacaa
     7201 aacaaattca gagccagaaa gttttcgtaa actcttttga attaacgagt cgatttcatc
     7261 ggaaactatt ttaaccacag cgggtgcttt gaagtctcga atacttttaa cgtttggaat
     7321 cactttatgc ccgcgagctt ctgctaaaag agagacat
//
LOCUS       DAOOBB010000086         7342 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_12108_length_7342_cov_4.267874, whole genome shotgun sequence.
ACCESSION   DAOOBB010000086 DAOOBB010000000
VERSION     DAOOBB010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 7342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7342
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12108_length_7342_cov_4.267874"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PLT31_05220"
     CDS             <1..349
                     /locus_tag="PLT31_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HPW94575.1"
                     /translation="VGGPHTGGEDVGSETWQCSDYRTGKPATSYTDNHCSYATNEVAI
                     NWNAPFVYLAGAIEAINAGFKPDFAVFNSKNSLPQVRGFAAQPSSGPKLIFDGHRLLM
                     KHNGKEYNLKGVQ"
     gene            552..1505
                     /locus_tag="PLT31_05225"
     CDS             552..1505
                     /locus_tag="PLT31_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94576.1"
                     /translation="MFFDKFSFYLFVGVLLIFLPFCAQAESVQISGTYGNHVDDVTYQ
                     KVIVLTEMFLKENDYEVVKHKSLTRAVLYVSMDNAFYVIELRKIKDSKTISQEKRYVY
                     NEKDLDVTIENLVNNVFEKKEEYSEKPSYENEVSAESSSSNSPASYWVYKSIDFGLSG
                     NLLFPSFRHQEVVNVGFGIGWGIRLGVNYAAEEYLTFPIIMNFADSKINDPQTHISFN
                     AGIRYYWDPEYDFFVNPFIGISINNYNKYDMDDKNYDESMNLIGYNFGLEGGYRFVRR
                     DALDMSFILSYEMTLNEESNKAKLSNTISALLQLSIVLGRK"
     gene            1502..2536
                     /locus_tag="PLT31_05230"
     CDS             1502..2536
                     /locus_tag="PLT31_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94577.1"
                     /translation="MRKIIICLFLLSTVLCSREIQILSSNVIDVEDETIVLVEQRVLS
                     VLDSMGFEVVEESDLALRTVMMKLGEDYLLYFEMTEDGKVVRSQREKLESSQNLEVIV
                     SDLLDEMFFTQTGDYKEDNRVEGYKKNQGVSDRKDSVLKSIFEEDKDERRFYDDEEES
                     FAGYLVAGVFIIAYKLTLHILVSLSDYIGLEASLGGVSAFSLSDEGANSSMDYKVGAQ
                     VRSSLFFGFNERHSWGFGLNFVKNPSEILNQHLSFLSTHRFYVNKYFFFDFVWGYGFF
                     WYDAPLQIDESLQLGWSFGGDVGYNIIQGKKHWLSLVIGFQHIPRDDMAEKDELSLNL
                     VYTFRGYFEE"
     gene            2628..2951
                     /locus_tag="PLT31_05235"
     CDS             2628..2951
                     /locus_tag="PLT31_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94578.1"
                     /translation="MRFDAGANVMLKFENIVCRIVFLGLFFCANLFAAPMENARGTLF
                     ILLDGMAPKSEGLFNNYCLDYERSETWGKTGVAKYFQEYVANSKANIYSRSYWNPIAS
                     PSEMV"
     gene            3051..3512
                     /locus_tag="PLT31_05240"
     CDS             3051..3512
                     /locus_tag="PLT31_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94579.1"
                     /translation="MKKLLTRLFFFISCVVLCACPYVDHGTSKAARFTSYEEIQGCYE
                     GGTPPFDGYPAKKMYCTRICFDEDAAVISKKGLYITEKTIDSSVVDSIDFDTTYSATY
                     ELILDLDSDGTYFYSININNFDKLAYRENDAFNGNKSPGEYTKERIAICDF"
     gene            3500..4078
                     /locus_tag="PLT31_05245"
     CDS             3500..4078
                     /locus_tag="PLT31_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94580.1"
                     /translation="MRLLMLIVFLFFANANALYIALNVNDSRLQSKNWIYKPSGIIGF
                     SIESGFFDEPENLVDYKLSIGIKRFGYSFDNGDGVLSFWGVDLKPCTWSITYKKVMLE
                     AYPSISWHFAQQNLRKRMYEHGFKKKIDQKRFAPGYGYRLGYHINEHILVALYADYKI
                     ISWDYQDFSGVDSGLHPDYYMGGLGLSLQWKF"
     gene            complement(4346..5461)
                     /locus_tag="PLT31_05250"
     CDS             complement(4346..5461)
                     /locus_tag="PLT31_05250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPW94581.1"
                     /translation="MSKHLYFYSDALEWGGQEILAARIANILADKYQVHFFFSSEKFK
                     TALNSSVEKISLPYHSQNPFPIVRDRISKKHKKTEQIFRDYGAGSDNFKKLIICPGNI
                     ERCIPAITAATKLKLQIISYYPMAFTQKESKATLGSLRDRLALRIYPKITKWIVNTPY
                     QEQLLRRFIDIDTEVFQLPNPLTFKENDVPKKPREIKRILNIGRIYFGQKGQEIIPEI
                     SKRLPSNSAIQFEVIGHGPDAEALRNKITQEHLEKQITTIDWISPNEIQKKFREQADA
                     LLVTSKFESGPMVLFEALQCGVPVIAANEDYIKAYRLPGWMTFEPGNANDAVKKLNNL
                     PKDWNLEEFESTRKALFCGRTSGEFQQRVLDIFDALE"
     gene            5686..>7342
                     /locus_tag="PLT31_05255"
     CDS             5686..>7342
                     /locus_tag="PLT31_05255"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HPW94582.1"
                     /translation="MKKRILLASLLFSVCAFAAPNVLINEVMPSNINVIMDDVNDFPD
                     SWIELYNTTSSSIDIQGWYVSDDILQKTKWKINSSVVIPANGYVLLYLDESENIPQHA
                     TFRLDINGSTVFLTSNDGSTLIDSITYIKMPPNLSYGRTTNGKAWFKKASPGKVNVNS
                     DAIINNQLAPVPEFSLLAGVYKGAINVSLSLPAGYSGLKIYYTTDGSEPNEQSSIYSG
                     PLSFQKSTTLRAKVIDAAYLPRVSSTRSYIITDRDLTLPVLSITTDSSYLFGPDKGIF
                     VWGKSYTDQYAEETEEHLYRKYSNLYHNWRRPMNLEYFDGSNHASVINQLGEMRISGG
                     GSRYEHEQKSFIIYANKRFGTKRYYYPFFNGKSSVEKGNGYKSLVVRNAGNDCSRAFL
                     RDAFIQNLYGGKTNVDYLAYQPVIVYINGEYWGIQNIRERSEDDYYLANYNLEQDAID
                     MYDNNQLQNGTETAFNALKNLVSGSFTYEELNEHIDIDNYLRYNVLYLFVWNPDWPGN
                     NNVFWRPSDGKFRWVLKDLDWGFGLDRGFSGDTAANSAFYILNP"
BASE COUNT         2253 a         1137 c         1542 g         2410 t
ORIGIN      
        1 tgtgggtggc ccacacactg gtggtgaaga tgttggttct gaaacttggc aatgtagcga
       61 ttatcgtacg ggaaaacctg ccactagtta cacagacaat cattgcagtt atgctacaaa
      121 cgaagtggct attaactgga atgctccatt tgtttatttg gctggcgcta ttgaagccat
      181 caatgccggt ttcaaaccag attttgctgt atttaattca aaaaattcgt tgcctcaagt
      241 tcgtggtttt gccgctcaac caagtagtgg tccaaaatta atttttgatg gtcatcgtct
      301 cttgatgaag cacaacggca aggaatacaa cttgaagggc gttcaataaa aatattttta
      361 gcgaacgttc aaaaagcact ctacaaaatt gttcataata tttcgtacac tataaactcc
      421 gactagctcg gagtttttta tggaaactat agtctgtgtg tttttcaaaa ttcaacttgc
      481 tgaaaaaaag aaacggcaaa gaataagtaa attctactca ccattttagg aaagagttta
      541 tatttacctt tatgtttttt gataagtttt ctttttattt gtttgtaggg gttttgttga
      601 tctttttgcc tttttgtgcg caggcagaaa gtgtgcaaat tagtggaacg tatggaaatc
      661 atgtggatga tgtcacttat cagaaagtga tcgttttgac agaaatgttt ttaaaagaaa
      721 atgattacga ggtggtaaaa cacaaatctt tgacgcgggc tgtgctctat gtgagcatgg
      781 acaatgcctt ttatgtcata gaattgagaa aaataaaaga ttcaaagacg attagccaag
      841 agaagcgtta cgtttataat gaaaaagatt tggatgtgac aatagaaaat ctagtgaaca
      901 acgtttttga aaagaaggaa gagtatagcg agaaaccgag ttatgaaaat gaggtttctg
      961 cagaatcgag ttcttctaat tcaccagcaa gctattgggt atataaaagc attgatttcg
     1021 ggttaagtgg aaatctgtta ttcccttcat ttcgtcatca agaggtagtg aatgttgggt
     1081 ttggtatagg ctggggcatt cgtctagggg ttaactatgc tgcagaggaa tatttaactt
     1141 tcccaatcat aatgaatttt gcagacagta aaatcaatga tccacagaca catatatcat
     1201 tcaatgctgg aatacgttat tattgggatc cagagtatga tttttttgta aaccctttta
     1261 taggtataag tattaataat tacaataagt acgatatgga tgacaagaat tatgacgaaa
     1321 gtatgaattt aatcggatat aattttggtt tagaaggtgg ttatcgattt gtaagaaggg
     1381 atgcattaga tatgagtttc attctttctt atgaaatgac tttgaatgaa gaatctaaca
     1441 aggcaaaatt atcgaataca atttcggcct tgcttcaact aagtattgtt ttaggaagaa
     1501 aatgagaaaa ataattattt gtttatttct gcttagtaca gttctttgtt caagagaaat
     1561 acagattctt tcatcgaatg tgatagatgt agaagatgaa accattgttt tggtggaaca
     1621 gcgtgttctc tctgtattgg atagtatggg ttttgaagta gtggaagagt cagatcttgc
     1681 actgcgtacc gtgatgatga aattagggga agactattta ttgtattttg agatgacaga
     1741 ggacggaaag gttgtaaggt cacaaagaga aaaattggaa tcgtctcaaa acttagaagt
     1801 catagtctcg gatttactag acgaaatgtt ttttacccaa acaggggatt ataaagaaga
     1861 taatcgtgta gaaggttata aaaaaaatca aggcgtttca gatagaaaag acagtgttct
     1921 taaaagtatt tttgaagaag ataaagacga gagaagattc tatgacgatg aagaagaatc
     1981 cttcgctgga tatcttgtgg caggagtttt tataattgca tataaattga cattgcatat
     2041 tcttgtttca ttaagtgatt atattggatt agaagcttct ttaggaggag tcagtgcatt
     2101 ttctctgtct gatgaagggg caaatagttc catggattat aaagtgggag cacaagtaag
     2161 aagtagctta ttttttggat tcaatgaacg tcattcttgg ggatttgggt taaattttgt
     2221 aaaaaatcct tctgaaatac tgaaccagca tttatctttt ctttcaacac atcgttttta
     2281 tgtaaataaa tatttcttct ttgactttgt ctggggttat ggattttttt ggtacgatgc
     2341 cccattacag attgatgaat ctttacaact aggatggtct tttggaggtg atgttgggta
     2401 taacataatt caaggaaaaa aacattggct atctttggta ataggatttc agcatatacc
     2461 aagagatgat atggctgaaa aagatgaact ctctttgaat ctcgtatata cattccgtgg
     2521 ttattttgag gagtagggtt gttgtattat ataattcagc taagttgtaa agaaaaaatt
     2581 acaagctagt cacctacata attaaaaaat aattctataa tcaatttttg cgttttgatg
     2641 caggagcaaa tgtaatgctt aagtttgaaa acattgtttg tagaattgtt tttctggggc
     2701 tgtttttttg tgcaaatcta tttgctgctc caatggaaaa tgcacgtgga actttattca
     2761 ttttattaga tgggatggct ccaaaaagtg agggattgtt taataattac tgtttggact
     2821 atgaaagaag tgagacatgg ggaaaaacag gagttgctaa atattttcag gaatatgtag
     2881 cgaattcaaa ggctaatatt tattcgcgtt catattggaa tccaatcgct agtccttctg
     2941 aaatggtgta gtgtccatca aaaacatgac attgtcagca aataactatt tggtgctgaa
     3001 acagaatgga attcttttgc aaaaattaaa attggagtaa ggtagctaat atgaaaaaat
     3061 tattaacacg tttatttttt ttcatatctt gtgtagttct ttgtgcgtgc ccttatgtag
     3121 atcatggtac ttcaaaagcg gcacgattta cgagttatga agaaatacaa ggatgttatg
     3181 aagggggtac tcctcctttt gatggttatc ctgcaaaaaa aatgtattgt actagaatct
     3241 gttttgatga ggatgccgcc gtgatttcaa aaaaaggcct gtatattaca gagaaaacta
     3301 tagattcttc tgtggtggat tcgattgact tcgacactac ctattcagcg acatatgaat
     3361 tgattcttga tttagatagt gatggaactt atttttattc tatcaacatt aataatttcg
     3421 ataaattagc ttatcgtgaa aatgatgcat tcaatgggaa caaatcacca ggtgagtata
     3481 ccaaggagag gatcgctata tgcgactttt aatgctaatc gtgtttcttt tttttgcaaa
     3541 tgccaatgct ctctacattg ccttgaatgt aaatgacagt agactacaat caaaaaattg
     3601 gatttataaa ccgtctggaa ttattggatt ttcgattgaa tcaggttttt ttgatgaacc
     3661 tgaaaattta gtggattaca agttgtctat aggaataaaa agatttggct attcttttga
     3721 taacggtgat ggtgttctat cattttgggg agtagactta aaaccatgta catggtcgat
     3781 tacttataag aaagtgatgt tagaagctta tcccagtata tcttggcatt ttgcgcaaca
     3841 aaatttaaga aaacgaatgt atgaacatgg attcaaaaaa aagattgacc aaaaacgctt
     3901 tgcacctggt tatggatatc gtttgggtta tcatataaat gaacacatac ttgtagctct
     3961 ttatgcggat tacaaaatca tcagttggga ttatcaagac tttagtggtg tcgattctgg
     4021 attgcatcca gactattata tgggaggcct aggcctttct ttacagtgga aattttaaaa
     4081 gctgcatccc gtgaatgcgg tactgcgcaa tcaacgcaat ccagcggttg tctatggtaa
     4141 cggtatctga aataggtggg attttgtcga gtcgttccat cttgaaaaac gaaagcattt
     4201 ccatgatact gtttatgctt gcgatttgct gattgatgct cgtgttgatg ccttatggtc
     4261 ggtgaaagcg atatagtcgc ccacttgaat tcatttcctc taatataaaa agattggagt
     4321 ctctgatatt acgttataac aagtattatt cgagtgcgtc gaatatgtcg agaacgcgtt
     4381 gctggaattc tccgctagtc cttccgcaaa agagtgcttt gcgcgtggat tcaaattctt
     4441 cgaggttcca atctttgggg aggttgttca attttttaac ggcgtcattt gcattgccag
     4501 gctcgaaagt catccagcca ggcagacgat aggccttgat gtagtcctcg ttcgcggcga
     4561 taacgggaac gccacactgt agcgcctcga aaaggaccat cggcccactt tcgaatttcg
     4621 aagtgaccaa gagagcgtcg gcctgttcgc ggaatttctt ctggatctcg ttaggactaa
     4681 tccagtcgat tgtagtgatt tgcttttcga gatgttcctg cgttatttta ttgcgtaacg
     4741 cttccgcatc gggaccgtga ccaatgactt caaactgaat ggcgctgttt gatggtagtc
     4801 gctttgatat ttcgggaatg atttcttggc ctttttggcc aaagtaaatt cttccgatat
     4861 taaggattcg cttgatttct cgtggttttt ttggcacatc gttttctttg aatgtgagtg
     4921 ggtttggcaa ctggaaaact tcggtatcga tatcaataaa acgacgtaaa agctgctctt
     4981 ggtaaggcgt gttgacaatc cacttggtaa tttttggata gattctcaat gcaagtcggt
     5041 cgcgaagact tccgagcgtc gctttagatt ccttttgtgt aaaggccatc gggtagtacg
     5101 aaataatctg tagtttcaat tttgtagcag cggtgatggc gggtatgcaa cgctcgatgt
     5161 tcccagggca gatgatgagt tttttaaaat tgtcgctacc agcaccgtaa tccctaaaaa
     5221 tctgttcagt tttcttatgc ttttttgaaa ttctgtctcg aacaatgggg aacggattct
     5281 gcgaatggta ggggagactt attttttcga ccgaggaatt taaagcggtc ttaaatttct
     5341 ccgatgaaaa aaagaaatgc acttggtatt tatcggcaag aatattcgct atacgggcgg
     5401 caagaatttc ctgaccgccc cattccagcg catcgctata aaagtacagg tgcttgctca
     5461 taatgtacaa tataaaaaat attgtctaag tataacttga atgatgcttt ctacgtcgaa
     5521 gtattcagat gtaatgagct agctcacttt atatgtgatg aaaaagtaca attcagtata
     5581 tatttaagaa cgtaaaaaac agagttgttt aaaaaatgaa acgtcatttg ctttgttgaa
     5641 ttatagcctt tttaaaatgc tcataagctg atgacctttt ctatgatgaa aaaaagaata
     5701 cttcttgcct ctctactgtt ttctgtttgt gcatttgcgg cgccaaatgt tcttattaat
     5761 gaagtgatgc ctagtaacat caatgttatt atggatgatg tgaatgattt tccagatagc
     5821 tggatagaat tgtataatac aacaagttct tcaattgata ttcaaggttg gtatgtttct
     5881 gatgatattt tgcaaaaaac aaaatggaaa atcaattctt cggttgttat tcctgcaaat
     5941 gggtatgtgc ttctctattt agacgaatca gaaaacattc cacaacacgc tacttttaga
     6001 ttagatatca atggcagtac tgttttcttg acatcaaacg atggcagtac attaatcgat
     6061 tccatcactt acatcaaaat gcctcccaat ctttcttatg gtcgaacaac taatggaaag
     6121 gcgtggttta aaaaagcaag ccctggcaaa gtaaatgtaa attctgatgc cattataaat
     6181 aatcagcttg ctccagttcc tgaattcagc cttttggctg gggtttataa aggggcaatc
     6241 aatgtatctc tttctctacc tgcagggtat agcgggctta aaatttacta cacaacagat
     6301 ggttctgaac caaacgaaca atcaagtatt tattcaggcc cattgtcatt tcaaaaatca
     6361 accacgctac gtgccaaagt gattgatgcc gcttatctac caagagtttc atcgactaga
     6421 agttacatca ttacggatag agatttgact cttcctgtat tatcgattac gaccgattct
     6481 tcgtatttat ttggcccaga caagggcatt tttgtatggg ggaaaagcta cacagatcag
     6541 tacgccgaag aaacagaaga gcatctgtat cgaaagtata gcaacttgta tcacaattgg
     6601 cgtaggccaa tgaatttgga atattttgat ggaagtaacc atgcctctgt cattaaccag
     6661 ttaggtgaaa tgagaatttc tggtggagga agtcgttatg agcatgaaca gaaaagcttt
     6721 atcatttatg ccaataaacg ctttggtact aaacgatatt attatccatt tttcaatggc
     6781 aaatcatctg tagaaaaagg aaatggttac aaatcgttag tcgttcgaaa tgctggcaat
     6841 gactgttctc gagcgttctt acgtgatgct tttattcaaa atctatatgg tggaaaaacg
     6901 aatgtagact acctcgctta tcaaccagtt attgtttaca ttaatggtga atattggggt
     6961 attcaaaata ttcgcgaacg aagtgaagat gattattatc tcgccaatta caatttagaa
     7021 caagatgcca ttgatatgta tgataataat caattacaaa atggcacaga aacggctttc
     7081 aacgcattaa aaaatttagt ctctggatcc ttcacctatg aagaactcaa tgaacatatt
     7141 gatattgata attatttgcg atataatgtt ttatatctct ttgtttggaa tccagattgg
     7201 cctggaaata ataacgtatt ttggagacca agcgatggca aattccgttg ggttctaaaa
     7261 gatcttgact ggggatttgg tcttgatcga ggcttctcag gagacacggc ggcaaatagt
     7321 gctttttata ttcttaatcc tg
//
LOCUS       DAOOBB010000087         7308 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_12181_length_7308_cov_5.263477, whole genome shotgun sequence.
ACCESSION   DAOOBB010000087 DAOOBB010000000
VERSION     DAOOBB010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 7308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7308
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12181_length_7308_cov_5.263477"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="PLT31_05260"
     CDS             <1..1050
                     /locus_tag="PLT31_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94583.1"
                     /translation="SASADWASIYHSIIAFDETGLPDGNRPGYVQPIITQLGTVLNGN
                     WVHSANVPQSFSFDAGLPITLSPITDKDRKYTASNVPTIFGENLPTYNYNANIPCSTS
                     PFCHEISGNETLNSLGLFSYPIAQAAVSYYHARVVLRGMWLPAISELKAFSLFGFGLQ
                     YSFGYLFQYALPKELQTLNVSLAFGYNSSSIGYTPEDYTGQLDLSVSSNIFQMVIGYS
                     PLSFIEVMLSLGYETASMKSGGTLVSPTNQTILPTLSVDGTNGFRLGLEVSFTLGNSY
                     HPVIGANFGTRTSFNTNILYFKQTFGTDPTPEEIAKKKKIKKETAEKENRPAMTEMDY
                     SAFDNSTDSTKTDGN"
     gene            complement(1047..1802)
                     /gene="truA"
                     /locus_tag="PLT31_05265"
     CDS             complement(1047..1802)
                     /gene="truA"
                     /locus_tag="PLT31_05265"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HPW94584.1"
                     /translation="MMRYRFRCEYLGSAFFGWQEQNEAGITKFITVQSELQNAFQIAL
                     REKVHLVGSGRTDTGVHARGQCVHFDWDHEPFDVLKVERSINGLTKRVIRIRDLEPCA
                     IDFHARHDAISRYYQYTIFTRPVALLRDFGWECGTVPLDLEVMEEECQSFVGYHDFIH
                     FCIPRNDGKTTFCDLSEFRMERVNEYTIRFHIRGNRFLHRQVRAMVGTLFDIGRGRYP
                     KGTIRNIFENQFKGERTWAPPQGLVLEDVVYRD"
     gene            complement(1805..2746)
                     /locus_tag="PLT31_05270"
     CDS             complement(1805..2746)
                     /locus_tag="PLT31_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94585.1"
                     /translation="MKKFVVPVILTLLIVGLWFYLKTDSKSNFADIDAALFLQTRSKI
                     DSLEIELWNHHKNPEMRAKVLGELDLRWEELQRLKSPLPVSSSDPESQSASINMQIGM
                     WSFIVILVVIFILAIVVILLKKKRDAITRQMEQIRAEQRFKAPKGGFQDDPSFVNRTR
                     VRRSIIDDAKAYAEEEKQKVSEVSPQDIAFEDENGVPENKIYVYDPEDRPPLRPTAKE
                     RITSAMQSLSDALVRPKGMSREKTMRVRAQSRNTLSNPSANNPLDVTRFDQERSEKER
                     VLQLMRRGYTTSEIARRMQISQEQVDVVIREIRDTGA"
     gene            complement(2866..>4011)
                     /locus_tag="PLT31_05275"
     CDS             complement(2866..>4011)
                     /locus_tag="PLT31_05275"
                     /inference="COORDINATES: protein motif:HMM:NF019365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HPW94586.1"
                     /translation="FIPHFSSSSAFPSSSSFSVYKPGDYILGVDISKFQEYESRGVLF
                     YDTDRQKKTIFQILKNHGINYIRLKTFVDPSAMYGYAASGCGETSSESFGDKAHIMAY
                     AKKVKEEGFGFLLDIHYSDNWADPGKQIIPERWRSVNSIDVLSDSVYAYTYDLLSSLK
                     SIGALPDMVQVGNEITDGLLRNVPTSETNCWGEPSSAASSTVSGRLSTQSGNFSKLIK
                     AGIKAVHDVDSRIKTVLHIENAVEHGSWWLSVVVDQQKINFDILAFSAYTAYGHGVAS
                     DWKSFISTQSSKYPNLQFLIAEYNGGISASTWTSGKTKEINLMLKETPKAIGSFYWEP
                     ALYGEWGGAAFEWQDNNLYAIPSAFSEFDEIRILPKEPPKRQSDIRF"
     assembly_gap    4012..4111
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4112..4404)
                     /locus_tag="PLT31_05280"
     CDS             complement(<4112..4404)
                     /locus_tag="PLT31_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94587.1"
                     /translation="MLFLKISVLLLYIILFGSCSETKVTDVDLSSSSFEDVLYLSSED
                     LEPVLESSSSVYENNSSFELPISSLEQPHFSSSETNLNLSSSMELLSSSMFIP"
     gene            complement(4600..6273)
                     /locus_tag="PLT31_05285"
     CDS             complement(4600..6273)
                     /locus_tag="PLT31_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HPW94588.1"
                     /translation="MNIPEPSNFIQEIIVDDLKTGKRNAVHTRFPPEPNGYLHIGHAK
                     SICLNFSTAAKFGGKTNLRFDDTNPSKEEVEYVDSIRDDVHWLGFDWEEREYFASDYY
                     DQIYDFAEQLILKGKAYVCELKGDAVSEYRGTLTEPGKPSPWRERPVSENLDLFRRMK
                     AGEFEEGACFLRAKIDMAHPNMNMRDPAVYRIKKASHHRTGDKWCIYPMYDFAHPISD
                     WIENITHSICTLEFEAHRPLYDWFLQALELENRPQQIEFARLNLSYTMMSKRRLLELV
                     QNKVVMGWNDPRMPTISGLRRRGYSPSSIREFCDRIGISKADSMVDINLLYFCIREEL
                     NASAQRAMAVLNPVKVVIDNWEEGRFEEIEVENNPENPAQGKRKILFGREIFIESDDF
                     MENPPKKYFRLHPDGEVRLKGAYFIKCVRVDKDDQGNILEIHCTYDPASRGGESPDGR
                     KVKGTIQWVSVPHAVSAEVRLFDHLFTLENPADVPEDKVWSDYLNPNSMIKCEAKLEP
                     MMGKATLADRYQFMRLGYFCLDSEENKQGKLVFNRTVELKDSWGKMQAK"
     gene            6494..7111
                     /locus_tag="PLT31_05290"
     CDS             6494..7111
                     /locus_tag="PLT31_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HPW94589.1"
                     /translation="MNLSLQQQLTRLLDEINNSGKTPHLLLHCCCAPCSSYVLEYLSP
                     YFVISVFYYNPNISPPSEYEQRLNEMKRFVQEFKAPNPIHLIEGEYEPKRFYSTVKGL
                     EEEPEGGERCVKCFELRLSEAAKKAKEVGADYFASTLTISPMKNASLLNKISEECGTI
                     YNVQNLPSDFKKKNGYKRSIELSKEYNLYRQDYCGCIFSKKEKET"
BASE COUNT         2242 a         1484 c         1242 g         2240 t
ORIGIN      
        1 tctgcttctg ctgactgggc ttcgatttac cactcgatta tcgcttttga tgaaacaggt
       61 ttacctgatg gtaatcgccc tggctatgtt cagcctatta ttactcaact tggaactgtt
      121 ttgaatggta actgggttca tagtgcaaat gtgcctcaga gtttttcttt tgacgcaggc
      181 ctacctatta ctctaagccc tattaccgat aaggatcgaa aatataccgc ttctaatgtt
      241 cctactattt ttggagaaaa tctacccaca tataattaca atgctaacat tccatgtagc
      301 accagtcctt tctgtcatga aatcagcggt aatgaaacat taaatagctt aggcttattt
      361 tcatatccca ttgctcaagc cgccgtgagt tattatcatg cgcgtgttgt tttgcgtggg
      421 atgtggctcc cagccatcag cgaactaaaa gcgtttagtc tttttggatt tggtcttcaa
      481 tatagctttg gttatttatt ccagtacgcc ttacctaaag aacttcaaac cttaaatgtg
      541 agtttagctt ttggatacaa ttcttcctct attggctata cacctgaaga ttatactggc
      601 caactggatt tatctgtttc ctcaaatata ttccaaatgg tgattggtta ttctcctctc
      661 tcttttatag aagtgatgct ttcacttggt tatgaaactg cttctatgaa atctggtggc
      721 accttagttt ctccaacaaa tcaaactatt ttaccaacgc ttagtgttga tggtacaaac
      781 ggttttcgtt taggtcttga agtttcattt actttaggta attcctacca tccagtgatt
      841 ggcgctaatt ttggcacaag aacatcattc aataccaata tcttatactt taagcaaacc
      901 tttggaacag accccactcc agaagaaatt gctaaaaaga aaaagataaa gaaagaaacc
      961 gctgaaaaag aaaatcgccc agcaatgact gaaatggatt attctgcttt tgataattca
     1021 accgattcta caaaaacaga cggcaattaa tctcgatata caacatcttc aagaacaagg
     1081 ccctgtggtg gggcccacgt tctttcgcct ttaaattggt tttcaaaaat attgcggatt
     1141 gtccctttgg gatatcgtcc acgaccaata tcaaataaag tacctaccat cgctctcact
     1201 tggcgatgta agaaacgatt tcctctaata tgaaatcgaa tagtatattc attcactcgt
     1261 tccattctaa attcagaaag gtcacaaaaa gtcgttttgc catcattacg tggaatgcaa
     1321 aaatgaataa aatcatgata cccgacaaag ctttgacact cttcttccat gacttctaaa
     1381 tctaggggaa cagtcccgca ttcccaacca aaatctcgta gtaaagccac tggacgtgtg
     1441 aatatggtgt attgataata acgactgata gcatcatgac gcgcatgaaa gtctatagcg
     1501 caaggttcta aatctcgaat ccgaatcact cgcttcgtta aaccattgat agaacgttct
     1561 acttttaaaa catcgaatgg ttcatgatcc caatcaaaat ggacacactg cccacgagca
     1621 tgaacgcctg tatctgttcg cccagaaccc actaaatgaa ctttttcacg aagggctatt
     1681 tgaaaagcgt tttgtaattc tgattgaacg gtgatgaatt tggtgattcc agcttcattt
     1741 tgctcttgcc agccaaaaaa agcactgcct aaatactcgc accgaaaacg gtagcgcatc
     1801 atgtttaggc cccagtatcc cgaatttctc ggatgacaac atccacttgt tcttgagaaa
     1861 tctgcatacg tcttgctatt tctgaagtgg tatatccacg acgcatgagt tgtaaaactc
     1921 tttctttttc agaacgttct tgatcaaaac gagtcacatc caaaggatta tttgctgaag
     1981 gattacttaa agtgtttcgg ctctgtgcac gcactcgcat cgttttttct cgagacatgc
     2041 ctttcgggcg aacaagcgca tcggaaagag attgcatcgc cgaagtgatg cgttcttttg
     2101 ctgtagggcg aagaggaggt ctatcctcag ggtcatatac ataaatttta ttttctggaa
     2161 caccgttttc atcttcaaaa gcaatgtctt gaggagacac ttcgcttact ttttgtttct
     2221 cttcttcagc ataggctttt gcatcatcta taatagaacg gcgaactcgc gtgcgattca
     2281 caaaagaagg atcgtcttga aagccgcctt ttggagcctt aaaacgctgt tccgcacgaa
     2341 tctgctccat ttgtcttgtg atcgcgtctc gctttttctt taacaagata actactattg
     2401 caagaataaa aataacgaca agaataacaa tgaaagacca catccctatc tgcatgttaa
     2461 ttgaagcact ttgagattca ggatctgagg aagaaactgg aagcggggat tttaatcgtt
     2521 ggagttcttc ccatctaaga tctaactcgc caagcacttt ggcgcgcatt tctggatttt
     2581 tgtgatgatt ccagagttcg atttctagtg aatcaatctt agaccttgtc tgaaggaata
     2641 atgcagcatc tatgtctgca aaattcgatt tagaatcagt ctttaaataa aaccataggc
     2701 caacaataag caatgttaaa ataacaggga caacgaattt cttcatataa ttaatgtagt
     2761 aaaaactgca gaagagatca gctactcttt tcttcttctc ttaaaatagc aattgatttc
     2821 tctttgaaat gaacctaggg gacacttaaa ctgataattc tttcatcaga acctaatgtc
     2881 actttgtctt ttagggggtt cttttggaag aattcttatt tcatcaaact cagagaaagc
     2941 ggaggggatt gcatacaaat tattatcttg ccattcaaaa gcagcacccc cccattcccc
     3001 ataaagagca ggttcccaat aaaaagagcc aatcgctttt ggtgtttctt ttagcatcaa
     3061 gttgatttct tttgttttcc cagaagtcca cgtggaagca ctaataccac cattatactc
     3121 cgcaattaaa aattgcagat tagggtattt actggattgc gtgctaataa aagatttcca
     3181 atctgaagct actccatgtc cataagctgt ataagcagaa aaggcaagaa tatcaaaatt
     3241 gattttttgc tgatccacca ctacactcaa ccaccaagag ccatgttcaa ctgcattttc
     3301 aatatgaaga actgttttaa tgcgagaatc aacgtcatgc acggctttaa taccagcttt
     3361 aattaatttg gaaaaattcc ctgactgcgt acttaatcgc ccactaacag tagaagatgc
     3421 tgcactagat ggttctcccc aacaatttgt ctcagaagtt ggtacatttc gaagcaaacc
     3481 atctgtaatt tcattaccca cttgcaccat atcgggcaat gcacctatac tttttagtga
     3541 agaaagcaga tcataagtgt aagcataaac cgagtcggaa agaacatcaa ttgaattcac
     3601 tgaacgccag cgttctggaa tgatttgttt tcctggatca gcccaattat cactataatg
     3661 aatatcgagt aaaaaaccaa agccctcttc ttttactttt tttgcataag ccataatatg
     3721 agctttatcg ccaaaagatt ccgaagaagt ctcaccgcaa ccagaagcgg catagccata
     3781 catcgcagaa gggtctacaa atgtttttag ccgaatgtaa ttaatgccat gattttttag
     3841 aatctgaaaa atagtcttct tttgacgatc agtatcgtaa aaaagaaccc cccgactttc
     3901 gtattcttga aatttggaaa tgtctacgcc taagatataa tcgccaggtt tgtaaacaga
     3961 aaaggaagaa ctactcggaa atgctgaaga ggaagaaaag tgcggaatga annnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntgcggaatg aacatggaag aggataatag
     4141 ttccatcgat gaagaaagat ttaagtttgt ttctgacgaa gaaaagtggg gctgttctaa
     4201 agaagagatt ggtaattcaa aagaagaatt attttcgtaa acagacgaag aagattctaa
     4261 aacaggctcc aaatcttctg aagataaata caaaacgtcc tcaaaagaag aagaactaag
     4321 gtctacgtct gtgactttgg tttcgctgca agaaccaaaa agaataatgt aaagtagcaa
     4381 gacgcttatt tttagaaata acacccaagt ctccttgaag aaaactcttt tctataaagc
     4441 tcttttaaac ataccttttt ttttaagaac agtactaata actgattttt gatgtgtaat
     4501 aaaaaatcgc cgaatacaag aaatattcga cgattttaat aatgcatata attttaagga
     4561 attcttaaag catatacaag cttattcaag acaaaagtct tattttgctt gcattttgcc
     4621 ccaagaatct tttaattcca ccgtacgatt gaagactaat tttccttgtt tattttcttc
     4681 tgaatctaag cagaaatagc caagacgcat aaactgataa cggtctgcaa gagtcgcttt
     4741 tcccatcatt ggttctagtt ttgcctcgca tttaatcata gaatttggat taagataatc
     4801 agaccagact ttatcttctg ggacatcggc tggattttct aatgtgaaaa gatgatcaaa
     4861 caaacgcacc tctgcactta ccgcatgagg aacactcacc cactggatgg tgcctttcac
     4921 tttacggcca tctggacttt ctccaccacg gctcgctggg tcataagtac aatggatttc
     4981 aagaatattt ccttgatcgt ctttatctac tcgaacacac ttgataaagt aagccccttt
     5041 taagcgcact tctccatctg ggtgtagtct aaaatatttt ttaggtggat tttccatgaa
     5101 atcatcactt tcaataaata tttctcgacc gaataatatt tttcttttcc cttgagcagg
     5161 attctctgga ttattttcta cttcgatttc ttcaaatcgg ccttcttccc agttatcaat
     5221 cacaaccttt acagggttaa gtacagccat cgcgcgctgt gcagatgcat tcaattcttc
     5281 tcgaatgcaa aaatacagaa ggttgatatc gaccatacta tcggcttttg aaataccaat
     5341 gcgatcacaa aactcacgga tactagaagg agaataacca cgtcgacgaa gtccgctaat
     5401 cgtaggcatt ctagggtcat tccagcccat cacaacttta ttttgcacaa gctcaagtaa
     5461 acgcctttta ctcatcatcg tataagacaa attcaaacga gcaaattcaa tctgttgtgg
     5521 acgattttct aattctaagg cttgtaagaa ccaatcgtac aaaggacgat gagcttcaaa
     5581 ttcaagagta caaatggaat gggtaatatt ttcaatccag tcgctaatag gatgagcaaa
     5641 atcgtacatt ggataaatac accatttatc accagtccga tgatggcttg ccttcttaat
     5701 acgataaact gctggatcgc gcatattcat gttagggtga gccatatcga ttttcgctct
     5761 taaaaagcaa gccccttctt caaactcacc agccttcatg cgacggaaca agtctaagtt
     5821 ttcagaaaca ggtctctctc tccaaggact aggcttacct ggttcagtta aagtaccacg
     5881 atactcacta acagcgtcgc cctttagttc acacacatac gccttgcctt tgagaatcaa
     5941 ttgttctgca aagtcataaa tttgatcgta ataatcgctg gcgaaatatt cacgttcttc
     6001 ccaatcaaaa ccaagccaat gcacatcgtc acggatggaa tctacgtatt caacttcttc
     6061 ttttgatgga ttagtatcat caaaacgtaa attggttttc ccgccaaatt ttgcggctgt
     6121 tgaaaaattc aaacagatac ttttagcatg acctatgtgc agatatccat ttggttctgg
     6181 aggaaaccgt gtatgcaccg cattacgttt tccagttttt agatcatcta caattatttc
     6241 ttgaataaaa ttagaaggct cagggatatt catactacat ctcaaaataa atttttaaca
     6301 aaacttgttc ctataattaa tgaatttgac cttttaggat aaaaccatta ttagaggatc
     6361 ttttgtataa atttagaaaa gtcttatcac ttttaaaagc gcttctcaaa aggacgcctc
     6421 taaaaaatga tttgcgagag cctggttttc ttgattttta gaacggtcct cttttttttg
     6481 ttattttttg tttatgaacc tttctttaca gcaacaattg actcgtctat tagatgagat
     6541 taacaattca gggaaaaccc ctcatttatt acttcattgt tgttgcgctc cctgtagtag
     6601 ctatgtttta gaataccttt cgccttattt tgtgatttct gttttttatt ataacccaaa
     6661 catttctcct cctagcgaat atgaacaacg gttgaatgag atgaagcgtt ttgtccaaga
     6721 attcaaagcc ccaaatccca ttcatttaat tgaaggggaa tacgaaccca agcgttttta
     6781 ttccaccgtt aaaggccttg aagaagaacc agaaggtgga gagcgatgtg tgaagtgttt
     6841 tgagctacgc ctttcagaag cggcaaaaaa agcaaaagaa gtgggagccg attattttgc
     6901 atcgacactc accattagcc ccatgaaaaa tgcctctctc ttaaataaaa tcagtgaaga
     6961 atgcggtacc atatacaatg ttcaaaacct accatctgat tttaagaaga aaaatggtta
     7021 taaaagatct attgaattat caaaagaata caacctctat cgccaagatt attgcggttg
     7081 tattttttca aaaaaagaaa aagaaacctg attcaacatc tctaaaaact cacactcatc
     7141 accaaattat tctgcaccat attttccctg ttttatttcg caaattattt tttgcatctc
     7201 ttcaatgccg tctataatta aatgcattac acaaaaaaac tgtactttct tttatagaag
     7261 ttctttttta agaactcgtt ttataactat attcttaata tgatttct
//
LOCUS       DAOOBB010000088         7294 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_12206_length_7294_cov_3.863655, whole genome shotgun sequence.
ACCESSION   DAOOBB010000088 DAOOBB010000000
VERSION     DAOOBB010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 7294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7294
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12206_length_7294_cov_3.863655"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1348)
                     /locus_tag="PLT31_05295"
     CDS             complement(<1..1348)
                     /locus_tag="PLT31_05295"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HPW94590.1"
                     /translation="MYLFIVLTLIMGIGEIIAQEDSSNVIFDDSKVHEYTLTFYDTDY
                     ATKLEANYFNDAGYLPAKFSDGTITLDSVGVRYKGNSSYNAASSSPKKPLKIKFNEFI
                     SGQKYYGIKVLNFSNGYGDPTFLREKIAYDISSKYIPSPRSSFAAITIGSDYIGLYTQ
                     IEQVNEQFLNRVYQDKTLNLYKASDAGASLVYSDNNPNSYASEMELKTNEDLNDWSGM
                     VSFLKYINNTDNESFCSTYSQYMNPDNVAPFLAFMMVMSHFDSYIGSGRNFYLTQMNA
                     AGYMSFVPWDLNLTFGGYPNGWDVYKQSAITTSNIDQRPLFKKLMGCEEFKYKYLAYI
                     KRMITTDASEESIQKAIDKHVSIIQPFVEADNNKFFSLSDFTTNLNSKLRTSSGSIPG
                     LTEFSTNRNTFLLSEIEGILPEEYELSIDKNNHDLAAKTSIRHRQIVFPSQRANTEK"
     gene            2382..3479
                     /locus_tag="PLT31_05300"
     CDS             2382..3479
                     /locus_tag="PLT31_05300"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPW94591.1"
                     /translation="MKIIFWIGFVLMIMGCADKGTSVNTPAEDISDDTPISSSSSLTN
                     SSIQELSSSINISSSLIHSSSSMLYSSSSVINSSSSSSLWVLSSSSQNFLFSDGQVRI
                     PDGCGSIYWSVSEEKTCVQDFWVDTTEVTIGAYQKLMGDEIPSLFHNGFYLWQNPSGE
                     GYADDGSLCPNCPVDGVTFYEAILYANEMTKKYGNPNDTIYTYTAINTSDTKGSLQRV
                     KPTEVTGLENLQITFNKNGYRLPTRGEFSYIYYQGVFTVYPWDDLYSNTQESYSAYDL
                     HSWNNRNSGKVTQEVATKLPTSWHLYDLIGNAFEWTTTPDIDNPGNYLAIGGSVIING
                     GGLEDIRLDSEHAGFRLVRGESHFRFIEYTD"
     gene            3591..5237
                     /locus_tag="PLT31_05305"
     CDS             3591..5237
                     /locus_tag="PLT31_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPW94592.1"
                     /translation="MRIVIIGAVAAGTSAAAKARRNNETAEIVIYEKNNYISYSGCGM
                     PYYLGDEVVDLQKLTPRNPDFFKTKYNVDVLIRHEVMSISPTEKKITVKNLESGAVIV
                     DYYDKLVIATGASATVPPILGADREHVFVLRNINDMLRIKDFINAKKISKVAIVGTGF
                     IGLEVCENLARLGISVTLIEKLPQVTPGLDRDMAIHVLHHLKEKGIRVILGASIESIE
                     AETINLSNGEKVDASMVLLSTGVRPNTALAKAAGISLGTANAIQVNSKMQTNIIDIYA
                     CGDCIEHFHLITNKPVYRPLGSTANKTGRIAGDQITGGSLEFRGILGTGIFRVFEMTV
                     AMTGLSEKEALDEGFDPVICHNIKPNKPEYLNGKEMVIKGVADRKTGRLLGAQIVGKE
                     GVDKRIDVFATAITFQAKVEDLFHIDLAYAPPFSTTKDPVMYTGMILENIIHRNRPVI
                     TKTELQQLQKQHPETHIMDARAVAQFKKDHLENAENMMPENLRKKAAKLDPEKPYIVY
                     CNKGTTGNAAQNILLNNGFKKVFNLSGGFKQYKAEQEDSL"
     gene            5337..6347
                     /locus_tag="PLT31_05310"
     CDS             5337..6347
                     /locus_tag="PLT31_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="expansin-like protein"
                     /protein_id="HPW94593.1"
                     /translation="MHYIVFLFILFFFSSCSSDKPSAVDSTLSSSSFEISSSIFSSST
                     IEPHSSSSKNPVINSSSAETPKESSSSVSSSSVEKSNSSSSAKTPSSSSDSGICTSCD
                     SYTALPPEIVENGGSGSITTYGSITAKETSQGGACNYGETNIQYYAAIHVHQIPGDFL
                     GPWKNGLACGACVSVKAKTPTGWKSTIVRITDKCPDDNCGVDLGGAPASDIMGIIVGR
                     YEGEWEFVPCKGIENVYGDSTSIYTKEGTNSFWSVIQVRNPPDALESITLVNLKDSTT
                     YNLELAIEAENYWKVPSEVLQSEDSFSITVSYRSGNPETWFLKGNELSQEQKTFYYYE
                     YK"
     gene            complement(6460..>7294)
                     /locus_tag="PLT31_05315"
     CDS             complement(6460..>7294)
                     /locus_tag="PLT31_05315"
                     /inference="COORDINATES: protein motif:HMM:NF017536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HPW94594.1"
                     /translation="QPAIDGAGVHLRRVLGLRTIESFDPFLMLDGFDSLNPEDYIKGF
                     PWHPHRGIETVTYLLKGKIEHGDSLGNKGIIGEEQCQWMTAGSGIIHQEMPQASEQML
                     GCQLWVNLPQKNKMTSPAYNDIRPENIIRIQEENAKVGILSGSYQGHKGAYDAKYIDV
                     QYLDILLKPNSVWKYKETPNDQTLFLYLIQGTLAANDALDQFEEKACAILMKPSSNDA
                     SSFDEVIVKSGPEGARFFLIAAKPLHEPVAWGGPVVMNTKEELNLAFEEIKNNTFIKH
                     G"
BASE COUNT         2277 a         1300 c         1308 g         2409 t
ORIGIN      
        1 acttttctgt atttgctctt tggctaggaa aaacaatttg tcgatgacga atgcttgttt
       61 tagcagctaa gtcatggtta tttttatcaa tagaaagctc atattcttca ggaagaatac
      121 cttctatttc actaagaaga aaagtatttc tatttgtgga aaattcagta agcccaggaa
      181 tactccctga agatgttctt aattttgaat taagatttgt tgtaaaatca gatagagaaa
      241 agaacttatt attatctgct tctacaaatg gttgaatgat actaacatgc ttatcgattg
      301 ctttttgaat ggattcttca gaagcatctg tggtgatcat tcttttaata taggctaggt
      361 acttatactt aaattcctca catcccatta acttcttaaa taatggacgc tggtctatgt
      421 tacttgtagt gatagcactt tgtttatata catcccatcc atttgggtaa ccaccaaacg
      481 tgagattcaa atcccaaggc acaaaactca tataccccgc ggcattcatt tgtgtgagat
      541 aaaaattacg cccacttcca atgtaagaat cgaaatgaga catcaccatc ataaatgcta
      601 agaatggggc gacattatct gggttcatat attgggaata tgtactacag aaagattcgt
      661 tatccgtatt attaatgtat ttgagaaaag aaaccattcc agaccaatca ttaagatctt
      721 catttgtttt taattccatt tctgaggcat aagaatttgg attattatcg ctatacacaa
      781 gagaggctcc tgcatcactt gctttatata agtttagtgt tttatcttga taaactcgat
      841 ttaaaaattg ttcattcact tgttctattt gagtatataa gccaatataa tctgatccaa
      901 tcgtgattgc agcaaacgag gagcgaggag aaggtatgta ttttgaggaa atatcatagg
      961 caatcttctc acgaagaaac gtaggatcac cataaccatt actaaagttt aataccttaa
     1021 ttccataata tttctgaccg ctaataaact cattgaattt gatttttaag ggcttctttg
     1081 gagaagaact tgcggcatta taggaagagt ttcctttgta tcgaacacct acactatcta
     1141 gcgtaattgt tccatcagaa aattttgcag gaagataacc cgcatcattg aaataatttg
     1201 cctccagttt tgtggcataa tcagtatcat aaaaagttaa ggtgtactca tgaacttttg
     1261 aatcatcaaa aataacatta gaggaatcct cttgtgctat aatctcacca atccccataa
     1321 tcaatgtgag aactataaac aaatacatag atttccatag taaaaaagtt aacagctaaa
     1381 ttaatctatc tattaaataa aataaaggat ttgttttttc acatccttct cctttttttt
     1441 acaatcactt aacatgaata aaattcgatg gtacgaaagt ccccatttaa ttatgttttt
     1501 tgatccgcgt tgtaaaccaa tacaatacta ttatcgcaaa tgagtttctt ttttcatgca
     1561 attgaattat atacaaatga ttctattaat gcacaattca tgtaaggtgc cttttcattt
     1621 tttcattggt tttcaataaa tgttttttca ttaaaactca ttaatagtat ggttgacgga
     1681 aatgtttttt tcacgattaa atctaaataa gtattattta attgataggt gttatttttt
     1741 tatttaagtc ttcgctattt gattgataat aacacgaaca cttagtagtt tactacttac
     1801 gtgagagtga tacccttgcg gtacaaattg acgctcgact ttatatgaaa aaaactgggt
     1861 ttggttagag gaacttttct tttctaacga gttcgtcggc aacatccatt tccgtctgct
     1921 atgcaactat tgttatttaa ttaattggtg ttttttttca cttgaatcta cgctatttga
     1981 ttactaataa cacgaacact tatatgcttt tacgtgaaac ttagtgcttt agcactgtag
     2041 ttgaaccatg catagctgca ttttacgtga gacttagcac ttttagtgct ttagtcggaa
     2101 ccatgcatag ctgtgagagt gatacccttg tggtagtgat ccccttgcgg gaaacacgaa
     2161 cacttaaaag tttacttttt atgtgagagt gataccttta tggtacaaat tgacgtttga
     2221 ttcttcatga aaaaactgag ttaggttaga tgagcgtctt gttcaaaatg caaattttat
     2281 ttttgtaaat cacagtgttt tgtttcttta tccaaacccc aaaaaataag actaaattca
     2341 tgaaaaaagt ttgaataaat gttcttggag ggcattttat tatgaagatc attttttgga
     2401 taggttttgt tttaatgatt atgggatgtg ctgacaaggg aacttctgta aatacaccag
     2461 cagaagatat ttcagatgat actcctattt cttcaagttc ttcgctcacc aattcttcta
     2521 ttcaagaatt aagttcatct ataaatattt ctagctcatt aattcactct agttcttcga
     2581 tgttatatag ttcttccagt gttataaact catcgtcatc ttcaagttta tgggttctta
     2641 gctcttcttc tcaaaacttc cttttttcag atggccaggt tcgtatccct gatggctgcg
     2701 gatccattta ttggtcggtt tctgaggaaa aaacatgtgt acaagatttt tgggtagata
     2761 caacagaagt caccatcggt gcctaccaaa aactcatggg cgatgagatt ccttctttat
     2821 ttcataatgg cttttactta tggcaaaatc caagcggaga aggttatgcc gatgatggaa
     2881 gtctttgccc taattgccct gtagatggag tcacttttta tgaagccatt ctttatgcta
     2941 atgagatgac aaaaaaatat ggaaacccta atgacaccat ttacacttat accgccatta
     3001 atacaagcga tacaaaaggc tctcttcaaa gagttaaacc aaccgaagtg actggattag
     3061 aaaatcttca aattacattc aataaaaacg gatatagact tcctacgcgc ggtgaattca
     3121 gctacatcta ttatcaaggc gtttttactg tttacccctg ggatgattta tactcaaata
     3181 ctcaagaaag ttacagtgct tatgatttac attcgtggaa taacagaaat agtggaaaag
     3241 taacgcaaga ggttgcaaca aaattaccta cttcttggca tttatatgac ctcatcggaa
     3301 atgcgtttga atggacaact acaccagata tcgataatcc tggcaattac ttagccatag
     3361 gcggctccgt aataattaat gggggtggat tagaagacat tcgactagat agtgaacatg
     3421 cagggtttcg tctcgttcga ggagagtctc attttaggtt tattgagtac acggattaaa
     3481 atttcttact ttgatttttt tttgataaag tttaattacc tttattaaaa ttaatgtgta
     3541 gtaaaatggc gtttgaggtt taaagtcact cccctatttg gagatgcgat atgcgtattg
     3601 taattattgg agccgttgcg gcaggaacat ctgctgcagc aaaagctaga cgtaacaatg
     3661 aaacggccga aatcgtaatt tacgaaaaaa acaactatat ttcttattct ggttgtggca
     3721 tgccctacta tcttggagat gaagtggttg atctccaaaa actcacacct cgaaatccag
     3781 atttttttaa gacaaaatac aatgttgatg ttttaattcg acatgaagtc atgagcattt
     3841 ccccaacaga aaaaaagatt accgtaaaaa atcttgaatc aggagctgtg attgtagact
     3901 attatgataa gcttgtaatc gccacaggag cgagtgccac tgtaccgcct attctaggag
     3961 ccgatagaga acatgttttt gtattgagaa acattaacga tatgcttcgt attaaagatt
     4021 ttattaatgc taaaaaaata tcaaaagtgg caattgtagg aacaggtttt attgggcttg
     4081 aagtttgtga aaatttagca cgtttaggca tttcagtaac gcttatagaa aaactcccac
     4141 aagtgactcc tggcttagac cgtgatatgg ctattcatgt gttacatcat ttaaaagaaa
     4201 aagggattcg agtgattctt ggagcctcca tagagtcaat cgaagcggaa actattaatt
     4261 tatccaatgg agaaaaagtg gatgcctcta tggtattgct ttctacagga gtgcgcccca
     4321 atacagctct tgcaaaagct gcaggaatct cattaggtac tgcaaacgcc attcaagtca
     4381 attctaaaat gcagacaaat atcattgata tttatgcttg tggtgattgc attgagcatt
     4441 ttcacttaat aacaaataag cctgtatatc gtcctctagg ctctacggcg aataaaactg
     4501 gacgtattgc tggagatcag ataactggtg gatctcttga gtttcgtgga atcctcggta
     4561 caggaatctt tcgtgtgttt gaaatgacgg tagcgatgac tggactttct gagaaggaag
     4621 ctctagatga gggctttgat cccgttattt gccataatat taaacccaat aagcctgagt
     4681 atttgaatgg caaagaaatg gtaattaaag gagttgctga tcgtaaaacg ggccgattgc
     4741 ttggtgctca aattgttggt aaagaaggag tagacaagcg aattgatgtt tttgcaacag
     4801 caattacttt tcaagccaaa gtcgaagatc tttttcacat agatctagct tatgctccac
     4861 cgttttcaac aactaaagat cctgtgatgt atactgggat gatactagaa aatataatcc
     4921 acaggaatcg tcctgtaatt actaaaacag agctacaaca actccaaaag caacacccag
     4981 aaactcatat tatggatgca cgagctgtag ctcagtttaa gaaagatcat ttggaaaatg
     5041 cagaaaacat gatgcctgag aatcttcgca aaaaagcggc aaaactagat cctgaaaagc
     5101 cttatattgt ttattgcaat aaaggaacta caggaaatgc agcccagaat attcttttga
     5161 ataatggatt taaaaaggta tttaatctct ctggtggatt taagcaatac aaagctgaac
     5221 aagaagactc actctaaaat caaaatgatg ctttaaaacc aactattttt tggtttcatt
     5281 tgttacaaaa tatcttactt tgattttttt gataaagatt aagtactttt attaaaatgc
     5341 attacattgt atttcttttt atccttttct ttttttcttc atgcagtagt gataaacctt
     5401 ctgctgtaga ttctacattg tcttcctctt ctttcgagat ttcctcttct attttttctt
     5461 cttcaacaat agaaccgcat tcttcttctt caaaaaatcc cgttataaat tcttcttcgg
     5521 cagaaacccc aaaagaatct tcttcatctg tgagctcttc ttctgtagaa aaatcaaaca
     5581 gttcgtcttc ggctaaaaca cctagcagtt cttctgattc tggtatttgt acttcctgtg
     5641 attcttatac tgccttgcct ccagagattg ttgaaaatgg tggttctggg tctattacga
     5701 cttatggatc tattactgca aaagaaacaa gccaaggcgg cgcttgtaat tatggagaaa
     5761 caaatattca atattatgcc gccattcacg ttcatcagat tcctggagat tttttaggtc
     5821 catggaaaaa cgggctcgcg tgtggggcct gcgtttctgt taaagctaaa acgcctacgg
     5881 gatggaaatc aacgattgtt cgcattacgg ataaatgccc tgatgataat tgtggcgtag
     5941 atctcggtgg agctcctgct tctgatatta tgggaattat tgtgggccgc tatgaaggag
     6001 aatgggaatt tgtcccttgt aaaggaatcg aaaacgttta tggagactct acttctattt
     6061 acactaaaga aggaaccaat tctttttgga gcgtcatcca agtaagaaac cctcctgatg
     6121 ccctcgaaag cattaccctg gtaaatctaa aagacagtac tacatataat cttgaacttg
     6181 ccatagaagc ggaaaattat tggaaagtcc cttccgaagt tttgcaaagc gaagattcat
     6241 tttcgatcac agtttcttac cgttctggaa atccagagac atggtttcta aaagggaatg
     6301 aattaagcca agagcaaaag accttctatt attatgaata caaataaatg ctatttaatt
     6361 aaacgtttat aatcttccac acagacataa tataaagaat ctttttttat gatatcattc
     6421 tcacgttcat ttttgtttta atctcatagt aaataaaatt taaccatgct taatgaatgt
     6481 attatttttt atttcttcaa aagcgagatt gagctcttct tttgtattca tcacaactgg
     6541 accaccccaa gctacaggtt catgaagagg ctttgctgct attagaaaaa agcgtgctcc
     6601 ttctggcccc gatttcacaa tcacttcatc aaaagaagag gcatcgtttg aagagggttt
     6661 cataagaatg gcgcaggctt tttcttcaaa ttgatctaga gcgtcgttag cggctaatgt
     6721 accttgaatg agataaagaa acaaggtttg gtcattaggt gtttctttgt atttccatac
     6781 cgaatttggt tttaacaaga tatccaaata ttgaacatca atatacttgg cgtcgtaagc
     6841 ccccttatgt ccttgataac ttcccgaaag tatgccaacc ttcgcatttt cttcttgtat
     6901 gcgaataatg ttttctggtc ggatatcatt atacgcaggc gatgtcatct tatttttttg
     6961 aggaagattc acccataatt gacaacctag catttgctct gatgcctgag gcatttcctg
     7021 atgaatgatt cctgaaccag ccgtcatcca ctggcattgt tcttcaccaa taatgccttt
     7081 attgccgaga ctatcaccat gttctatttt gcctttcaat aaataggtaa ccgtttcgat
     7141 tccacgatgc ggatgccatg gaaacccttt aatataatct tcagggttga gagaatcaaa
     7201 accatctaac attaaaaatg ggtcgaatga ttcaatggta cggagtccta aaactcgacg
     7261 taaatgaacc ccagcgccat caatagcagg ttgg
//
LOCUS       DAOOBB010000089         7266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_12256_length_7266_cov_5.400083, whole genome shotgun sequence.
ACCESSION   DAOOBB010000089 DAOOBB010000000
VERSION     DAOOBB010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 7266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7266
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12256_length_7266_cov_5.400083"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="PLT31_05320"
     CDS             <1..727
                     /locus_tag="PLT31_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HPW94595.1"
                     /translation="GLYVSSNGSDYYLREVVQGVKTGAETSLKAPAGAFTSGFMTLAD
                     GSLAQGNLTVASPGAANGILKAGPVFINEIYYHPIEVLDPEQRLKEVEFLELVNKSDS
                     VINFNPIINTKPGYWKVDGIKMSFVPPASIPANGFALLIEPTADSAFFRQYNNVSANV
                     PIYYYQGKLSNRGELLVLKRPYGYDANNELYFSFEDAVLYSDDWDGTKEADGLGKSLH
                     RTDYSTMGYESGVWIAAKPTPGR"
     gene            857..1570
                     /locus_tag="PLT31_05325"
     CDS             857..1570
                     /locus_tag="PLT31_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HPW94596.1"
                     /translation="MLLDPLYMVILVFGLVLSGITSFMVSSRFKAGQKVQIHSGLTGA
                     QVAKAILADAGVTDVTIHETSGFLADHYNPLNKTLHLSRDVYHGTTASAAGVAAHEVG
                     HALQHAENYFPMWLRSFIVPAANIGSNLGPWLVIIGIVLMSVQSLGFGQSVAIFGVAL
                     FALSTVFTFVTVPVEFDASNRAKKRLQALSIVQQGREYKAVSAVLLAAGLTYVAAAIQ
                     SLMQLVYWAIRAGLLRNDD"
     gene            1582..1866
                     /locus_tag="PLT31_05330"
     CDS             1582..1866
                     /locus_tag="PLT31_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94597.1"
                     /translation="MAVKTISVECPCCKATLVVDPETGSVLSHREYKKEKLSLDDFLS
                     QQKNRAAELDAKFEAAKEKEKNKFDLIEKKFQAAKENKDLKDPPPTVLWD"
     gene            complement(1832..2929)
                     /locus_tag="PLT31_05335"
     CDS             complement(1832..2929)
                     /locus_tag="PLT31_05335"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HPW94598.1"
                     /translation="MVISEKEDYEVRIGAFEGPMDLLLYLVQKNEVNPGEISIAEITD
                     QYLFWIKDLAQADLSAAGDFLLMASRLMALKVRELLPKEAQSEEELLEFSEDREQLLR
                     EMLEYQRYKQVAKELQEKEFENFGTFYRGRLERSHNEEETLADANIWQLFRAYQKSLK
                     ARNAESIHHIELDYVTIEDRQQVISNYLAAHGRALFEDLLGRDQHPIVAAVTFMALME
                     MIKTDEVVFRQGEPFGPIWLYRKKNNLDYADEMANETVILSKDPEFKPGLIDALRERE
                     SSLAQNKAATIDAVMREAVLWASQGKMIQEDDLIAMLEGRISLSETNETPTEIQNDSS
                     AEASPAAENKILAENASIEEINPTERLEEDL"
     gene            complement(2948..4777)
                     /gene="glmS"
                     /locus_tag="PLT31_05340"
     CDS             complement(2948..4777)
                     /gene="glmS"
                     /locus_tag="PLT31_05340"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HPW94599.1"
                     /translation="MCGIVGYVGEQEVLPILLEGLKKLEYRGYDSSGISIICNQKIQT
                     EKASGKIVALEEKLHNKCFKGTIGIAHTRWATHGAPIESNAHPHCSYDGKISVVHNGI
                     IENYAILKSKLETEGITFVSDTDTEVIAHLIARLYKGDIKAAVLKAISKLEGAFGIAV
                     LCEDEPNVLVGARRGSPLILGIGDSNDYFLASDVSAIVHHTQKVVYLDDNDVVVIKGS
                     KYELLNMSNQKVQREVQEVEFDVESVAKGGFPHFMLKEIFEQPDSLRNTMRGRLLLAD
                     GTAKLAGLDANIKELRSINRIIIIACGTSYYAGMVGEYMIEDLAGIPVEVEYASEFRY
                     RNPIINPGTLVLTISQSGETADTLAALKEAQHKGATVLGICNAVGSSIARYSNGGVYL
                     HAGPEIGVASTKAFTSQVAVLAMIALLLGRQRHISFEKGIDIAKAIFDVPNLVQATLE
                     LSDQIAEIAKNFIHSKNFLYLGRHYNYPVAMEGALKLKEISYIHAEGYPAAEMKHGPI
                     ALIDENMPVVVIAPKDALFDKIVSNIQEIKARGGRIIAITTEQSTVLDQFADHVIQIP
                     QTLPMLMPLITCIPLQLLAYHIAVLRGNDVDQPRNLAKSVTVE"
     gene            4948..5829
                     /locus_tag="PLT31_05345"
     CDS             4948..5829
                     /locus_tag="PLT31_05345"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPW94600.1"
                     /translation="MNLKLLLSVGLTSIFLFGCNEMGKPAAKTALVTEKDNYSYALGA
                     SFGQQARALLIQRDSIDLDYAVFMQAFAERFEQDSAKLLMSDSALFAVLNDFSMKRQQ
                     EKLQKDSIAAEKNLQEQKAFLEKNKTEGGVITTESGLQYLVIIDGKGELPKETDIVSV
                     HYTGTLLNGTEFDSSIKRGAPAEFPLNAVIPGWTELLKLMKVGTKVKAWIPSDLAYGP
                     RGRQVIPGNSLLVFEMELLGVKASEQAAAPAAAPAKPATVKKTAAAKPAAAKPAPAAA
                     APAAAPAAAPAAPAAAQ"
     gene            5844..7229
                     /locus_tag="PLT31_05350"
     CDS             5844..7229
                     /locus_tag="PLT31_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPW94601.1"
                     /translation="MIRSVMPLTGNTSLIFFIGGILHRLFLFITIASLFFLASCGEAR
                     KIDELKKTVVSLEHELDSLQQENKKREENQLTNLQVVHDSVQAGEGLFQVLNRMGIND
                     EQRRKIVLSIQDSVELSILKVGQRFYAAKDSAQNVRIFRYEANPATIHLLFLNPETSL
                     FEYKLVSKPLVKKQSVFEGVLESGSTLNGLLFQVGIPGRMVGIVSGVLQCKVAFQHAQ
                     PGDRFRILLEESFYQDSIWISGKVLYAEFEGRVVGHHEAFRYEDPDPKSTFNAHYTAS
                     GEALIFDGLRYPLDRLHITSSFGSRIHPITGSRAMHNGVDYGSPRGAPVYAVAEGTVI
                     KSGFDQYSGNKIAIRHRDNSTSWYLHLDKRSVSVGTRVAPRQVIGRVGSTGRSTGPHL
                     HFGFTNAKGKWINPLTKTMIATPKLEGARLARLQVQVEEIKKELALTEAQEPQKANDS
                     LDVNVRMRVLP"
BASE COUNT         2125 a         1340 c         1487 g         2314 t
ORIGIN      
        1 agggctttat gtgagttcaa atggatctga ttactatttg agagaagtag ttcagggtgt
       61 gaaaactggt gctgaaacaa gtttaaaagc ccctgcaggt gcttttacaa gcggttttat
      121 gactcttgct gatggtagtt tagctcaagg taatttaacc gtcgctagcc ctggagctgc
      181 aaatggaatt ttaaaagcgg gtcctgtatt catcaatgaa atctattatc acccaattga
      241 ggttttggat cctgagcaac gtcttaaaga agtagagttt ttggagttgg tcaataaatc
      301 agactccgta attaatttta atccaattat taatacaaaa ccagggtatt ggaaagtgga
      361 tggcataaaa atgtcttttg tacctcctgc atctattcct gccaatggtt ttgctcttct
      421 tattgaaccc actgctgata gtgctttctt ccgtcagtat aataacgttt ctgcaaatgt
      481 tcctatatac tattaccaag gaaaactttc taatcgagga gaattattag ttttgaaacg
      541 cccttatggt tatgatgcga ataatgaatt gtatttctct tttgaagatg ccgttttgta
      601 ttcggatgat tgggatggaa cgaaagaagc ggacggctta ggaaagagct tacatcgtac
      661 agattattca accatgggtt atgaatctgg tgtttggata gcagctaaac caactcctgg
      721 ccggtaatta taaaaacgtt tttactcaat tagcagaaat ttagaagcca taaaaaagac
      781 cttgccttta aggcggtctt tttttgtatt ttttagttga aaaaggcttt tgggccattc
      841 attaaggaga atgaaaatgc ttttagaccc tttatatatg gttattttag ttttcgggct
      901 tgtcttatca gggattactt ctttcatggt ttctagccgt tttaaggcag ggcagaaggt
      961 tcagattcat agtgggctta ctggagctca agtggcaaaa gcgattcttg ctgatgcagg
     1021 agtgaccgat gtaacgattc atgaaactag cggcttttta gcggatcact ataatccact
     1081 caataagaca ttacatttat ctagagacgt gtatcatgga acgacggcga gtgctgcagg
     1141 cgttgccgct catgaagtcg ggcacgcttt gcagcacgct gaaaattact ttcctatgtg
     1201 gcttcggtct tttattgtcc cagccgccaa tattggatct aatttaggcc cttggcttgt
     1261 gattattggt attgttttaa tgagtgttca atcattaggt tttggtcaat ccgtcgccat
     1321 ttttggtgtg gctctttttg ctttgtcgac tgtttttact tttgtcacgg tgcctgttga
     1381 atttgatgcc tctaaccgtg ctaaaaaacg tttgcaggct ttgagtattg tgcaacaagg
     1441 tcgagaatat aaagctgttt cagcggttct tttggctgct gggcttactt atgtggctgc
     1501 tgcaattcaa tcattaatgc aactagtata ctgggctatt cgcgctggtt tattaagaaa
     1561 tgatgattaa ggatttttgt tatggcagtg aaaacaattt ctgtagaatg tccttgctgt
     1621 aaagcgactc ttgtggtaga tccagaaaca ggatctgttt taagtcatcg agaatacaaa
     1681 aaagaaaaac tttctttaga tgatttttta tctcaacaaa aaaatagagc cgcagagctt
     1741 gatgctaagt ttgaagcagc aaaagaaaaa gaaaaaaata aattcgattt gatagagaaa
     1801 aaatttcaag ccgcgaagga gaataaggat cttaaagatc ctcctccaac cgttctgtgg
     1861 gattaatttc ttctatcgaa gcgttttcgg ctagtatttt attttctgct gctggtgaag
     1921 cttctgctga agagtcattt tgaatttctg taggcgtttc atttgtttct gaaagagaaa
     1981 tgcgcccttc tagcatggcg atgagatcat cttcttgaat catttttcct tgagaagccc
     2041 ataagacggc ttctcgcatc actgcatcaa tagtggctgc tttattttga gctaggcttg
     2101 attctctttc acgaagagcg tcaattaatc ctggcttgaa ttcaggatct tttgaaagaa
     2161 ttaccgtttc atttgccatt tcatcggcat agtctaaatt attctttttg cgataaagcc
     2221 agatagggcc aaagggctca ccttgcctga acaccacttc atctgtttta atcatttcca
     2281 taagagccat aaacgttaca gcagctacga tcgggtgttg atctctgcct aacaagtctt
     2341 caaataaggc tcttccatgg gcagctagat agttgctgat tacttgctga cggtcttcga
     2401 tggtgacgta atctagttca atgtgatgaa tactttcggc attacgagct tttagacttt
     2461 tttgataagc tctgaatagt tgccaaatat tagcatcggc aagcgtttct tcttcattat
     2521 gagacctttc caatcggcct ctataaaaag taccaaagtt ttcgaattct ttttcttgaa
     2581 gctctttagc gacttgttta taacgctgat actcaagcat ttctcttaaa agttgctcgc
     2641 ggtcttctga aaattctaga agctcttctt cactttgtgc ttctttgggg agtagttctc
     2701 tgacttttaa ggccattaaa cggcttgcca ttaaaaggaa atcgcctgca gcagataagt
     2761 ctgcttgagc taaatcttta atccaaaata aatattggtc tgtaatttct gctattgaaa
     2821 tttcacctgg attcacttca tttttttgaa ctaggtaaag taaaagatcc attggaccct
     2881 caaaggcgcc aatgcgaact tcgtaatctt ctttttcaga aataaccata aaagaaaaga
     2941 taaataatta ttcgacagtg acgctctttg cgaggtttct tggttgatct acatcgttcc
     3001 cacgtagtac tgcaatgtga taagcgagta attgtaaggg aatacaagtg attaatggca
     3061 taagcatcgg aagagtctgt ggaatttgaa tcacatgatc agcaaattga tccaaaacgg
     3121 tactttgctc tgtagtaatt gcaatgatgc gaccaccacg cgctttaatt tcttgaatgt
     3181 ttgatacaat tttatcaaaa agggcatctt taggggcaat caccactaca ggcatgtttt
     3241 catcaattaa tgcaataggc ccgtgtttca tttccgctgc tgggtaacct tcagcgtgaa
     3301 tgtaactgat ttctttaagt tttaaagctc cttccatggc cacaggatag ttatagtggc
     3361 gccctaagta aagaaagttt ttagaatgaa taaaattctt tgctatttct gcaatctgat
     3421 cagatagctc aagtgtggct tgaacaagat ttggaacatc aaaaattgcc tttgctatat
     3481 caattccttt ctcaaatgaa atatgacgtt gcctacctaa caaaagagca atcatggcaa
     3541 gtacagcgac ttgagaagta aaggcttttg tactggcaac accgatttct ggccctgcat
     3601 gaagataaac accgccatta gagtaacggg caatagaaga acctacggca ttacaaatgc
     3661 caagaacggt ggcgccttta tgttgagctt cttttaatgc ggcaagtgta tctgctgttt
     3721 ctcctgattg agaaatggtg aggacaagtg tacctggatt aataatagga tttcgataac
     3781 ggaattcaga agcgtattct acttccacag gaatccctgc taaatcttcg atcatatatt
     3841 cgccaaccat acctgcgtaa taacttgttc cgcaagcaat aatgattata cgattaatgg
     3901 atctaagctc tttaatatta gcatctaagc ccgctagttt agctgttcca tcagcgagta
     3961 gaagtcggcc acgcatcgtg tttcgtaaag aatcgggctg ttcaaaaatt tcctttaaca
     4021 taaagtgagg gaaaccgccc ttggcaacgg attccacatc aaattctact tcttgtactt
     4081 cacgttgtac tttttgatta ctcatattta aaagttcgta tttcgaacct ttaatcacaa
     4141 caacatcatt gtcgtcgagg tacaccactt tttgagtatg atgaacaatg gcagatacat
     4201 cacttgctaa aaagtaatcg ttagaatcac ctatgccaag aattaaaggg cttccacggc
     4261 gtgcgcctac cagaacattg ggctcgtctt cacaaaggac agctatacca aaagcgcctt
     4321 ccaatttgct aatggcttta agaacggcgg ctttaatatc tcctttataa agtcttgcaa
     4381 ttaaatgcgc tatgacttct gtatcagtat cagagacaaa ggtaatacct tcggtttcta
     4441 gctttgattt taaaatggcg tagttttcga taatgccatt gtgaacaaca gaaattttac
     4501 cgtcatagct acaatgaggg tgagcattgg attcaattgg agccccatgg gtagcccaac
     4561 gagtatgagc aataccaatg gtgcctttga aacacttatt atgaagtttt tcttctagag
     4621 ctacaatttt tccagaggct ttttctgtct ggattttttg attgcaaata attgaaattc
     4681 cagaactatc atagcctcta tactcaagct tttttagtcc ttctaaaaga ataggaagaa
     4741 cttcttgttc tccaacataa ccaacgatac cgcacatatt taactctgat aaaaataaac
     4801 ttcaccaaaa atacatataa tgggttctaa aaaacaacaa aatacgcttc gatcttgtca
     4861 gaattaagta aaaataaaat gtaaagactt tttaatcgct tctttttcta ttttagcgtg
     4921 catcagtttt ttcacaaaga ggattatatg aatctcaaat tattgttatc ggtagggtta
     4981 acctctatct ttttatttgg atgtaatgaa atggggaaac cagcagcaaa gactgcactt
     5041 gttactgaaa aagacaatta tagctacgct ttaggagctt cttttggtca acaagctcgt
     5101 gcattactta ttcagcgcga ttctattgac ttagattacg ctgtatttat gcaggctttt
     5161 gcagaacgtt ttgaacaaga tagcgccaag cttttgatgt ctgactctgc tttatttgct
     5221 gttttgaatg acttctctat gaaacgtcaa caagaaaagc ttcaaaaaga tagcattgct
     5281 gcagaaaaga atcttcaaga acaaaaagct ttccttgaaa agaataagac tgaaggtggt
     5341 gtgattacta ctgaaagtgg tttacaatat cttgtcatta ttgacggtaa gggtgaactt
     5401 cctaaagaaa ctgacattgt aagcgttcat tatacaggaa cacttttgaa tggaaccgaa
     5461 tttgatagca gcattaaacg tggtgctcca gcagaatttc ctttgaatgc agttattcca
     5521 ggatggacag aacttcttaa gttgatgaaa gtagggacaa aagttaaagc ttggattcca
     5581 agtgatttag cttatggtcc tcgtggtcgt caagttattc caggcaatag cttacttgtt
     5641 ttcgaaatgg aattattagg cgtaaaggct tcagaacaag ccgctgcacc agcggctgct
     5701 cctgcaaaac cagctacagt taaaaaaaca gctgctgcta aaccagctgc tgctaagcca
     5761 gcccctgccg ctgctgcccc agctgctgct ccagctgccg cccctgctgc tccagctgct
     5821 gctcaataat attttattag tcaatgataa gatcagtgat gcctttaacg gggaatacat
     5881 cattgatctt ttttattgga ggtattttgc atcgcttatt tttatttatt actatcgcta
     5941 gtttgttttt tcttgcttcc tgtggcgaag ctcggaaaat agacgaatta aaaaaaacag
     6001 tggtttctct agaacatgaa ctagattctt tgcaacagga aaataaaaag cgagaagaga
     6061 atcaattgac gaatcttcaa gtggtgcatg attcggttca agcaggagaa ggactctttc
     6121 aggttttaaa tcgaatggga attaatgatg aacaacgaag aaaaatagtt ctttccattc
     6181 aagatagcgt tgaattatct attctaaaag tgggtcaacg tttttatgcc gcaaaagatt
     6241 cagcacaaaa tgttcgcatc tttagatatg aggcaaatcc agcaacgatt catttactct
     6301 ttttgaaccc agagacttct ttatttgagt ataaactagt ttcaaaaccg cttgtgaaaa
     6361 agcaatctgt atttgaaggc gttttagaaa gtggttctac attaaacggt cttttatttc
     6421 aggtggggat tcctggacgt atggtaggga ttgttagtgg tgttcttcag tgtaaagtgg
     6481 cttttcaaca tgctcaacct ggggatcgct ttcgcatttt attagaagaa tctttttatc
     6541 aagactccat ttggattagt ggtaaggttc tttatgctga atttgaaggt cgagttgttg
     6601 gacaccatga agcgttccgt tatgaagatc cagatcctaa aagtactttt aatgcgcatt
     6661 atacggcttc aggagaggcg ttgatttttg atggattaag atatccttta gatcgattac
     6721 acatcacaag ctcctttggc tctcgcattc atcctattac ggggtctcga gccatgcata
     6781 atggggtaga ctatggtagc ccaaggggcg cccctgttta tgcagttgca gaaggaacgg
     6841 ttattaaatc aggttttgat caatatagcg gaaataaaat agcgattcgt catcgggata
     6901 attcaacaag ctggtatttg catttagata aacgaagcgt cagtgttggt actagagtag
     6961 cgccaaggca agtcattgga cgagtgggta gcacaggccg aagtacaggt cctcatttac
     7021 attttgggtt tactaatgca aaagggaaat ggattaatcc tttaactaag acgatgatag
     7081 ctacaccaaa attagaagga gctcgtttag ctcgcttgca agtacaagtg gaagaaatta
     7141 aaaaagaatt ggcccttacg gaagcgcaag agccacaaaa agcaaatgac tcgcttgacg
     7201 tgaatgtacg tatgcgagtc ttgccttaat tcaattaaat cttttctaaa cgtttaatct
     7261 taaagg
//
LOCUS       DAOOBB010000090         7262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_12266_length_7262_cov_4.669072, whole genome shotgun sequence.
ACCESSION   DAOOBB010000090 DAOOBB010000000
VERSION     DAOOBB010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 7262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7262
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12266_length_7262_cov_4.669072"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PLT31_05355"
     CDS             complement(<1..358)
                     /locus_tag="PLT31_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPW94602.1"
                     /translation="MFLEMMSFDFMQNAFIAGLLVAIACGIMGSFVVVNKLTGLSGGV
                     AHASYGGVGLACFFGFSPMLGALGFALACALLMGVLTWKNRTRSDTLIGVIWAFGMAL
                     GVVLTDLTPGYASDMMS"
     gene            complement(358..1383)
                     /locus_tag="PLT31_05360"
     CDS             complement(358..1383)
                     /locus_tag="PLT31_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HPW94603.1"
                     /translation="MFKKIIDSYYKPVEYPALFDQMETWSKTKPLKGLRVLDASPIFR
                     NTLVKYDALLAAGASLSVGLSKKMPHDELIVKTIKESGLSVYSPEEVPQTWDLVLDCA
                     AQFAFLESKYGFVELTRSGVSIYEKSEKPVFVADSSEIKKIETSLGTGESYFRAMHQL
                     GFSSWDQKKLLVFGSGKVGMGIIMNALSLGIKTSVVTDLSLYENALLEKGMTSVVDLK
                     NDEGVVSKIKEADYIVSATGIKNALDRPSWVLALLNSKAIFANMGVEDEYGPLISDSM
                     VLNEKKPLNFLLEEPTHLKFIDASLALHNVLSLALLNTSGKGICDIPKALEDHILNVT
                     RQHGEIL"
     gene            complement(1376..2056)
                     /locus_tag="PLT31_05365"
     CDS             complement(1376..2056)
                     /locus_tag="PLT31_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HPW94604.1"
                     /translation="MIINEESKIWLFDYDLTLYGEEERHVLDSLDHRITLFVKRTTGV
                     SLEQASEIRKKYWVDYGTTLAGLHAEYQVDPNDFFDFIHLNDGLIFPKKAPQKRILLE
                     SIKGKKYVFTNARKDWSDAGLSSMDIADCFDGVMDLKKLDWKGKPSVDAYDKMEAFLK
                     KASINSFDENPSQIIFLEDSIRNLQEAHERGWTTIFVNATQQKPDWVDYQLDHLQSLK
                     LLLEDPHV"
     gene            complement(2053..3360)
                     /locus_tag="PLT31_05370"
     CDS             complement(2053..3360)
                     /locus_tag="PLT31_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HPW94605.1"
                     /translation="MLYWDSLLSATRYAHPAVRDPNRSEFHRDYDRIVFSTAFRRLGR
                     KTQVHPFSVNDHVHSRLTHSIEVSSVGRSLAITVFHLIKQHLPKHFNEYHFGMILQSA
                     CLAHDIGNPPFGHAGEAAIREWFRKNRASAPLKDLSDKEMKDFENFDGNAQGHRILSK
                     LEYHFLDGGMRLTYATIGSMIKYPRLASFGTPTSIFSTESDLYRETANVLGLPEIETG
                     RWVRHPLSFLLEAADDICYSILDVEDAIELGILSFAEVRSMFIYLCGDEVNVDKEFEE
                     NGQNFRDFLSSIRGHAIQNLIDDIAQVFVNHYDEIMAGKLDKSLIDLSRSETMEGIRI
                     AKRLGRERIYPDRRKTELEVGSYTTLSTVLDAFISGVYEFRLYGKNSYRADRIVRLIG
                     QAKIGQSVSSVEAYHQVLDFVSGMTDNYAIYLARQIGGLVNTL"
     gene            complement(3375..5003)
                     /gene="ccsA"
                     /locus_tag="PLT31_05375"
     CDS             complement(3375..5003)
                     /gene="ccsA"
                     /locus_tag="PLT31_05375"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HPW94606.1"
                     /translation="MKKVVIFTLFFISLLWSEGIYDQGRIKPFDSFSRDILFSFNGKS
                     SFQGLSAKELILKIIQDPASTNEFELFKINRAEVAELLHLDSKKRYHSYSNLKSSRFL
                     LEQYNERKDDHPVTLELKKLYREMRSYESLAHALDYKRPLIQIDSDSLKSELGLESSK
                     NMYSPQELLTKIRELDSASCSDSALWWRDSILFQTFIGMQESPLRIYPSSEKDGLVSA
                     WDYLWHGKAIPSYSLPIEENSFRLRAEIYYHRLNLPQKALILLGLAIFLGLIHRVFQK
                     PWIQFSEFILLGLAGFLLTITLGLRSYIMQAPPLGSLYEVVLLVLVFISLILGISFLK
                     KWLTFALIPSSLLMFILLFFAQNALVQGDSFRVLPPLLNSSFWLTTHVFTIALGYSGM
                     ILSGLWAHLCMLKPSQQNLRMLYGILVFGVSFTLIGILLGGIWADLAWGRFWGWDPKE
                     CAALFVVIWAMISLHAKAGRFLTPEGFVFFSSWNIIIVALCWFGVNLLGIGLHSYGSQ
                     RGIGLSLFYFIIADALLIFFLAWIQRRRNHTPIE"
     gene            complement(5000..6040)
                     /locus_tag="PLT31_05380"
     CDS             complement(5000..6040)
                     /locus_tag="PLT31_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94607.1"
                     /translation="MFKGTKNLKRAASLQVTIVLLAFLALLLFWGILYQSKVGVELAT
                     ERFFDSYFIWVFDVIPLPGLYSLLVVSAIHLVLSMLYRIPRQKKSIGLYLMHFALLVL
                     ILGGLLGSFSRVSMKGYGLVGGEIPVVFEHSSDEYEVVLNDSLSVSLPPWGKLGAFQL
                     GPYDFKIIKSCEEGSPIVKVEEDHKILKNASGFQGFECKSQKESVLFPGVILHIKHRE
                     FLHEEKVLLGLNERRPYVLTGGESLRLSNIREKMPFEIQVVSADQTGADVIIMSGRET
                     FPRRIALNVPYKSSHYSIYFTSVIDSFDDQQIVLFQARKDFFDSVPYLFTILMLAGFA
                     FHYLIFCPRKKS"
     gene            complement(6033..6950)
                     /locus_tag="PLT31_05385"
     CDS             complement(6033..6950)
                     /locus_tag="PLT31_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPW94608.1"
                     /translation="MIDFIGDIHGHYDRLIALLEKLGYTKKNGVYKHPSRRVIFLGDY
                     LDRGPDARSVIRLVRSMVEAGEAEALIGNHEFNTLCFWTPRAEGGYLREHSINKILIH
                     TETLCSYQHHDLEFMEMLRFLYTLPLYIETDLFRAQHACFDSSYVDILKKENCNSVQN
                     EEILKRMIDKNDPLYKPVELLLKGPEMALPKGISYRDSEGVLRTRTRVCWWENPATAS
                     SAELFMQPENEILDVEVPSEIRKWNYYHESERPVFFGHYWFTGEVRLLKPNVCCLDYS
                     VASYKKNGKLVAYRFDGESTLTREHFVYV"
BASE COUNT         2530 a         1539 c         1176 g         2017 t
ORIGIN      
        1 aactcatcat atcacttgca taaccaggag tcaagtcagt taagacaaca cctaaggcca
       61 taccaaaggc ccaaatcact cctattaatg tatccgatcg agttcgattc ttccaagtta
      121 aaactcccat caataaagca caagcaaggg caaacccaag agcaccaagc ataggagaaa
      181 agccgaaaaa gcaagctaag ccaacgccac cataagaagc atgagcaacg cctcccgata
      241 aaccagtgag cttattcacc accacaaaag agcccataat accgcaagca atagcgacta
      301 acaaacctgc gataaaggca ttttgcataa aatcaaacga catcatttct aaaaacatta
      361 taaaatttct ccatgttgcc gagtcacgtt taagatatga tcttctagtg ctttagggat
      421 atcacaaatt ccttttccag aagtattcaa taaagctaga ctaagaacat tatgcaaagc
      481 aagagacgca tcaataaatt ttaagtgcgt aggttcttcg agtaaaaagt ttaaaggttt
      541 tttttcattt aataccatcg aatctgaaat taaaggaccg tactcatctt ctacacccat
      601 attagcaaaa atagccttcg aatttaagag ggctaaaacc catgatggac gatctaaagc
      661 gttttttatc cctgtagcgg agacaatata atcagcttct ttaatttttg aaacgactcc
      721 ttcatcattt tttaaatcta ccacagaagt catccctttt tccaaaagag cattctcata
      781 taaacttaaa tcagtaacga cactcgtttt gataccaaga gaaagggcat tcatgataat
      841 acccatcccc acttttccac tcccaaagac aagaagtttt ttttgatccc aagaagaaaa
      901 gcctagttga tgcatggctc gaaaataact ttctccagtc cctagagaag tctctatttt
      961 ttttatctca ctagagtctg cgacaaatac aggcttttct gatttttcat aaatggaaac
     1021 gcctgatcga gtcaattcaa caaagccata tttagactct agaaaagcaa actgagcggc
     1081 acaatctaaa acaagatccc acgtttgtgg cacttcttct ggagaataaa cagaaaggcc
     1141 tgattccttg attgttttca caatcaattc atcatgaggc attttttttg aaagccccac
     1201 cgaaagagag gcgcctgcag caagaagagc atcatacttc actagcgtat ttctaaaaat
     1261 cggagaagca tctaatacgc gaaggccttt cagaggtttt gttttagacc atgtttccat
     1321 ttgatcaaaa agggctggat actccactgg tttgtaataa gaatcaataa ttttcttaaa
     1381 catgtggatc ctccaataat aattttaaag actgcaaatg atctaattga taatctaccc
     1441 aatctggttt ttgttgagtg gcatttacga aaatagtggt ccagcctctt tcatgagctt
     1501 cctgcaaatt gcgaatagaa tcctctaaaa aaatgatctg cgaaggattt tcatcaaagc
     1561 tattaatcga cgcctttttt aagaatgctt ccattttatc ataagcatct acagaaggtt
     1621 tccctttcca gtctaacttt ttcaaatcca taacgccatc aaaacaatcc gctatatcca
     1681 tcgaagataa acccgcatca ctccaatcct tgcgagcatt agtaaacaca tactttttcc
     1741 cttttatact ctcaagcaaa attctttttt gaggtgcttt tttaggaaaa attaaaccat
     1801 cattcaaatg gataaaatca aaaaaatcgt tcgggtccac ttggtattct gcatgtagcc
     1861 ccgctaaggt ggttccataa tccacccaat atttttttcg aatttctgaa gcctgttcta
     1921 aagagacacc agtcgttctt ttgacaaata aagtaatacg atggtctaat gaatctagta
     1981 catgacgttc ctcttctcca taaagagtta aatcataatc aaacaaccag atttttgatt
     2041 cttcatttat aatcacaacg tatttaccaa gccgccaatt tgtcgtgcca aatatatggc
     2101 ataattatca gtcataccac ttacaaaatc taaaacttga tgatatgctt ctacactaga
     2161 aaccgattgc ccaatttttg cttgcccaat caaacgcaca attcgatcag cacgataaga
     2221 gttttttcca taaagacgga attcataaac tcccgaaata aaagcatcta ataccgtact
     2281 gagtgtagtg tagctaccca cttctaactc ggtcttacga cggtcagggt atatgcgttc
     2341 acgccctaat cttttggcaa tgcgtattcc ttccatggtc tctgatcttg ataaatcaat
     2401 aagactcttg tctagctttc cagccataat ttcgtcataa tgatttacaa aaacttgggc
     2461 gatatcatca attagattct ggatcgcatg gccgcgaata ctcgataaaa aatctctaaa
     2521 gttctgccca ttttcttcaa actctttatc cacatttact tcatctccac agagataaat
     2581 aaacatagaa cgcacttcag caaaacttaa aatgccaagt tcgatcgcat cttccacgtc
     2641 taaaatagaa taacagatat catctgccgc ttctaataaa aagctgagtg gatgacgaac
     2701 ccagcgccct gtttctattt ctggtaaacc aagaacattc gctgtttcgc gatacaaatc
     2761 gctttctgta gaaaaaatac tcgttggagt gccaaaacta gcaagacgcg ggtacttgat
     2821 catcgagcca attgtcgcgt aagtgaggcg cattccacca tctaaaaaat gatattctaa
     2881 ttttgataaa atacgatgtc cttgagcatt gccatcaaaa ttttcaaaat ccttcatttc
     2941 tttatcggaa agatctttga gaggagcgga agctctattc tttcgaaacc actcacgaat
     3001 cgccgcttct ccagcatgac caaaaggagg atttccaata tcatgcgcta agcaggccga
     3061 ttgcaaaatc atcccaaaat gatattcatt aaaatgtttt ggtaaatgct gcttaattaa
     3121 atgaaaaaca gtaatagcaa ggcttcgccc cacactagag acttcaatac tatgcgttaa
     3181 acgactatga acatggtcat tcacagaaaa tgggtgaact tgagtttttc gacctaaacg
     3241 tctaaaagct gtggaaaaaa caatacgatc ataatctcga tgaaattccg aacgattcgg
     3301 atcacgtacc gctggatgag cgtaacgcgt agcagaaaga agagagtccc aatataacat
     3361 aagtcaaatt taaattattc tataggcgtg tgatttcgac gacgctgaat ccaagccaaa
     3421 aagaagatca gtaaagcatc tgcaataata aaatagaaaa gggaaagtcc aattcctctt
     3481 tgagaaccat aactatgtaa acctattcct aaaagattga ctccaaacca gcaaagggcg
     3541 acaataatga tattccatga ggagaaaaaa acaaagcctt caggcgttaa aaaacgacca
     3601 gctttcgcat gcagagaaat catcgcccaa atcaccacaa aaagagcggc acattcttta
     3661 ggatcccaac cccagaagcg tccccaagct aaatcagccc atatgcctcc taaaagaatc
     3721 ccaatcaaag taaagctcac cccaaaaact aaaatcccgt atagcatacg caggttttgc
     3781 tgtgatggct taagcataca taaatgagcc cataagccag ataaaatcat cccactataa
     3841 ccaagtgcaa tcgtaaacac atgagtggtt agccagaaag aggagtttag taaaggcggc
     3901 aagacacgaa atgaatcccc ttgtacaagc gcattttgtg caaaaaacaa aagaataaac
     3961 atcaagagac ttgaagggat caaagcaaaa gtcaaccatt tttttaaaaa agagatacct
     4021 aaaattaagg agataaagac tagaactaaa agcaccactt catataagct cccgagaggc
     4081 ggggcctgca taatataaga acgtagccct aaagtaatcg ttaaaagaaa accagcaagt
     4141 ccaagcaaaa taaattctga aaattgaatc caaggctttt gaaaaacgcg atgaatcaaa
     4201 cccaagaaga tggcaagccc caaaaggatt aaggcttttt gagggagatt caaacgatga
     4261 taataaattt ctgcacgtaa tctgaaacta ttctcttcta tcggcagaga ataagaagga
     4321 atcgctttcc catgccataa gtaatcccaa gcagaaacaa gcccgtcttt ttcagaactc
     4381 ggataaatac gaagcggcga ttcttgcatt ccaataaatg tttgaaagag tatagaatct
     4441 cgccaccaca aagcagaatc agaacaagaa gcggaatcaa gttctcttat ttttgttaaa
     4501 agttcctgag gagaatacat gttttttgaa ctctctagcc caagctctga ttttaacgaa
     4561 tcggaatcaa tttgtattag agggcgttta taatctaagg catgtgctaa tgattcgtaa
     4621 gagcgcatct cgcgataaag tttttttaat tctaacgtca caggatgatc atcttttcgc
     4681 tcattatatt gttctaaaag aaatctactc gatttaagat ttgagtaact atgataacgc
     4741 tttttagaat ctaggtgtaa aagttctgcg acttctgctc tattgatctt aaacagttca
     4801 aattcatttg tagaagcagg atcttgtata atttttagaa tcagttcttt cgctgataag
     4861 ccttgaaaag aagatttccc attgaaacta aataaaatat ctcggctaaa agaatcaaaa
     4921 ggcttgatcc ggccttggtc gtatatgcct tcactccaaa gtaaagaaat aaaaaaaaga
     4981 gtaaaaataa cgactttttt catgactttt tccttgggca aaaaataaga taatgaaaag
     5041 caaaaccagc gagcattaaa atcgtaaaca gatacgggac actatcaaaa aagtcttttc
     5101 gtgcctgaaa aagaacgatc tgttgatcat caaaggaatc aatcacagaa gtaaaataga
     5161 tgctataatg actcgattta taaggaacat ttaaggcaat tcgacgaggg aacgtttctc
     5221 tccctgacat aataatcaca tctgcacccg tctgatctgc agaaaccacc tgaatttcaa
     5281 aaggcatctt ttctctaata ttggaaaggc gaagcgattc tcctcccgta agcacataag
     5341 gacggcgttc atttaaacct aaaaggactt tttcttcatg aagaaattcc ctatgtttta
     5401 tatgaagtat tacaccagga aaaagcaccg attctttttg gcttttacat tcaaagcctt
     5461 ggaatccgct tgcatttttt aaaatcttgt gatcttcttc cactttcaca ataggagacc
     5521 cttcttcgca agacttgatg atcttaaaat cataaggccc taattgaaaa gcccctaatt
     5581 ttccccacgg tggtaaagat acacttaaag aatcgtttag aaccacttcg tattcatcag
     5641 aagaatgttc aaaaacaact gggatttccc ctcccacaag gccataccct ttcatagaca
     5701 ctcttgaaaa gctgcctaat aagcctccta aaatcaatac taaaagagca aaatgcatta
     5761 agtaaagccc aatcgatttt ttttgacgag gaattcgata gagcattgat aaaaccaaat
     5821 gaatagcact tactacaagt aaagaataaa gacctggtaa tggaataaca tcaaagaccc
     5881 aaataaaata cgaatcaaaa aaacgttctg tcgctaattc cactccgact tttgattggt
     5941 ataaaatgcc ccaaaaaagt aacaacgcta aaaaagcaag taacacgatc gtgacttgta
     6001 aactagccgc tctttttaga ttttttgtcc ccttaaacat agacaaaatg ttctctcgtc
     6061 aacgtagact ctccatcaaa acgataagcc actaattttc cattcttttt ataagaggca
     6121 acactataat ctaagcagca cacatttggc tttaaaagac gtacttctcc agtaaaccaa
     6181 tagtgaccaa aaaagacagg gcgttctgac tcatgatagt aattccattt tctgatctca
     6241 gagggaactt caacatctaa tatttcattt tctggctgca taaaaagttc tgcagaagac
     6301 gctgttgctg gattttccca ccaacaaact cttgttcgcg ttcttaaaac gccttcagaa
     6361 tctcgataag aaatcccttt cggtaaagcc atctctggcc ctttcagtag tagctcaact
     6421 ggcttataga gaggatcatt tttatcaatc atccgtttta atatttcttc attttgaacg
     6481 ctattgcaat tttctttttt taatatgtcc acataagacg aatcaaaaca tgcatgttgt
     6541 gctctaaaaa gatccgtttc aatataaagt ggtagcgtat ataaaaagcg aagcatctcc
     6601 ataaattcta aatcatgatg ctggtaagaa cacaacgtct ctgtgtggat caaaatttta
     6661 ttgattgagt gttctcttaa atagcctcct tctgcacgag gagtccaaaa gcaaagcgta
     6721 ttaaactcat gatttccaat taacgcttct gcttcccctg cttctaccat cgaacgaact
     6781 aaacgaatca cactccgtgc atctgggcca cgatccaaat aatcccctaa gaaaataact
     6841 cgacgcgaag ggtgtttata aactccattt tttttggtat agccgagttt ttctaataac
     6901 gcaattaagc gatcataatg gccgtgaata tcaccaataa aatcaatcat aaaaagcctc
     6961 taaaaataga tttgataaaa acaaaagtgg caagtattgg cgatatatgc caattttaac
     7021 aacgtacgac ctgcaaaata tcaaagtatt tttgtccatg aatgaatttt tagtgattcc
     7081 cataagatta aaggtacaaa aaagccttcc cccaatcagg gaaggccttt ctatcgaacg
     7141 cacaaaggga atctaaaaac aatgtgattc gatgaagctc aaaaaggatt ccatcatgca
     7201 aagaaaaatc ttattctgac ttcacccttt taagagtcat tctctcagaa attaacatta
     7261 tt
//
LOCUS       DAOOBB010000091         7022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_12769_length_7022_cov_5.239271, whole genome shotgun sequence.
ACCESSION   DAOOBB010000091 DAOOBB010000000
VERSION     DAOOBB010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 7022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7022
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12769_length_7022_cov_5.239271"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1192)
                     /gene="xseA"
                     /locus_tag="PLT31_05390"
     CDS             complement(<1..1192)
                     /gene="xseA"
                     /locus_tag="PLT31_05390"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPW94609.1"
                     /translation="MQEEIKSYPVSKYLTSIKNLITNQVPSVWIHGVLTQMTERGNMI
                     YLNLAEFVKDDVKPVATLPLYIFKSDYSLLKKKLASLPTPFELKEQLKVNLLIQADFY
                     IPYGKFQSKVIDIDPAYTLGELAITREAILKKLRTEGLLRKNAELPFPSLPLHIGLIT
                     GEKTAAYRDFCSQLKESPFAFQITEAFARMQGNETENSILEALGKLRSFPDIDVVCII
                     RGGGAKTDLNYFDSEALCRAIALYPTPVLTGIGHEIDQSLVDVVAWFACITPTACAKF
                     IVERVEGSWIKVQEMAFNIKNRIQMRIPREKEKLHYIQKTFFYNASKTVIREKERLLQ
                     FQSILKKEPLRYFKIEKEILQRFQLGLKNGSGKILNLEKAKFEILNAKIALSDPKSIL
                     KKGYS"
     gene            1368..1748
                     /locus_tag="PLT31_05395"
     CDS             1368..1748
                     /locus_tag="PLT31_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94610.1"
                     /translation="MRIKPLLTAFFLMMFMGCASNQALVNSSIQQAESLKTTAGESKS
                     LSVKTTLADEKLEEAKKQNEIGKTEEALLAAEESLLQYRLALAQSTHDALSKDYAALE
                     KEVQSAESYKNQLKSKLEQMRKEK"
     gene            1745..2758
                     /locus_tag="PLT31_05400"
     CDS             1745..2758
                     /locus_tag="PLT31_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPW94611.1"
                     /translation="MKLATLFYTSLLSASFIFASNSNTDSLSTKQAASWSVKACQESL
                     TNLNKEIPAEALDAKITLAEAQAKASLVAAQEIKNPKATSVAAFQKTCSLYVDLIQVQ
                     AKTQLLRNSLQSLWEKRTSTMQTAMATKDSIENFYKNKTSAFANALEEEKERLALTSE
                     ELIQKAKAREDSIKAAADEREAALQKALDEERQKAEARQQEAKNKLNELQSKLIQVTQ
                     DARGIILSMSDILFDVNKASLKGDLKTSLAKVAGILSVYQELNVSVEGHTDNTGSEEH
                     NLKLSEQRAQNVLDFLVNQGIEASRLTSKGYGMTLPIANNKTKEGRQKNRRVDLVIKD
                     KAL"
     gene            2763..2954
                     /locus_tag="PLT31_05405"
     CDS             2763..2954
                     /locus_tag="PLT31_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HPW94612.1"
                     /translation="MAKIVSAMEVRQNFGSLLNQVSLLGEEIVIERSGKSIARLVSIS
                     EPSLSKLDFRDIAKLPSIK"
     gene            complement(3020..4684)
                     /locus_tag="PLT31_05410"
     CDS             complement(3020..4684)
                     /locus_tag="PLT31_05410"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047334 - diphosphate-fructose-6-phosphate
                     1-phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HPW94613.1"
                     /translation="MSDNLSVLGKARAAYQPKLPSSLKNGALKVSINKGTATESVRDQ
                     EKIKALFPNTYGAPVVSFTPACDCSCTCESKAIRVGVVLSGGQAPGGHNVIAGIFDGL
                     QSISKNSVLFGFLGGPSGLENGKYIQIDAKLMDAYRNTGGFDIIQSGRTKLETTEQFD
                     RCIKVANDLKLDAITIIGGDDSNTNAAVLGEYFQAKGASCVVCGCPKTIDGDLKNEYI
                     ETSFGFDTAVKTYSELIGNIMRDANSAQKYWHFIKLMGRSASHIGLEAALQTHPNICL
                     ISEEVKAKQMKLSEVVKYVADIVAERASQGKNFGVALIPEGLLEFIPDVGVLISELSE
                     VLAHHEKEVEGLNTQDKVEKLCGWLNTESANVLKSLPAGIQAQLMLDRDSHGNVQVSL
                     IETEKLIIEMVAKELKKRSDFKGKFAALNHFFGYEGRCAAPSNFDADYCYSLGYAASV
                     LALNKMNGYMSSVRNLTKGIENWTAGGIPITMMMNIERRHGADKPVIQKALVELDGAP
                     FKYFAARRDEWAKTESYTYPGPIQYWGPSEVCDVTNFTIKLERGAM"
     gene            4816..5547
                     /locus_tag="PLT31_05415"
     CDS             4816..5547
                     /locus_tag="PLT31_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94614.1"
                     /translation="MKRLQCLTIILFVLSSFPLWAQNKDIRFVPESYSVGVIEQGDVR
                     HIVLKGVNTTSKTIVLESVMSQNTGSSNFKHPSVIKPNETITIEFDLNTSYMEGAFNH
                     SIVLVDTTGAFYTTTIEGDVQSPVFFSEKMFDAGYYKAREKREWTFYVWSTDKKTRPD
                     IELDSIAAKSFSATYKSVMLNIDKLDQIKEGGKVPALKVTLKTKGIPRDGIALKQKSL
                     RHIVAFKSKAHPKATPEILIVGYWK"
     gene            5605..5678
                     /locus_tag="PLT31_05420"
     tRNA            5605..5678
                     /locus_tag="PLT31_05420"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5639..5641,aa:Pro,seq:ggg)
     gene            complement(5717..>7022)
                     /locus_tag="PLT31_05425"
     CDS             complement(5717..>7022)
                     /locus_tag="PLT31_05425"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPW94615.1"
                     /translation="SFKRIWQKYASHKIGEDGFLIAFDDKNRVLLYPELSKILEQELY
                     ASDLGLKDFSAKTFSITKPTFITLNNKQYLAAYSYNPRYQIGILSLQSRDEVQSLIAA
                     VAYTMVFLLALTIVATILIILWLFKNFAFPLRNIIEHINYIASGNFDSEPLPEINKEN
                     ELGILTESFNQMQSIIQKQIQDLNLHKEHLELEVLERTRELEEAKNQLDLISRTDELT
                     KLPNRRDIREKIQQEANRAERTHRDFCFIFIDIDKFKDINDTYGHYCGDLVLKGVADI
                     IRKNLRKYDYVSRWGGEEFLTVLPETDLDGAKIVAERFRRKVAQMKIEFSDTIIGVTI
                     TLGVALYDQKLGVDKSIQLSDHALYKGKMNGRNQVVIWDPKDTSEDEYKMALLDQKAF
                     LYASCPIDNEEKNDPSDFEMDQQNEDDDEIEEIDDKETSSKE"
BASE COUNT         2265 a         1244 c         1299 g         2214 t
ORIGIN      
        1 gactataccc tttttttaaa atcgattttg gatcagaaag tgcaatcttt gcatttaata
       61 tttcaaattt ggctttttct aaattgagga tttttccaga gccatttttt aaaccgagct
      121 gaaaacgctg taagatttct ttttcgatct taaaataacg aaggggctct ttttttaaga
      181 tggattgaaa ttggagcaac cgttcttttt ctcgaatgac tgttttagag gcattataaa
      241 aaaaggtctt ttgaatataa tgaagtttct ctttttctct tggtattctc atttgaattc
      301 gatttttaat attgaatgcc atttcttgta ctttgatcca agacccttcc actctttcga
      361 caatgaactt tgcacaagcg gtgggagtaa tgcatgcaaa ccatgccaca acatcgacaa
      421 gactttggtc tatttcatga ccaattccag tgagcacagg ggttgggtag agggcgattg
      481 ctcggcataa ggcttcacta tcaaaataat taaggtcagt tttcgcgcca cccccgcgga
      541 taatgcaaac tacatcaata tctggaaaag atcttaattt accaagagct tctaaaatac
      601 tgttttcggt ctcatttcct tgcatgcgag caaaggcttc tgtaatttga aatgcaaaag
      661 gggattcttt tagttggcta caaaaatctc gataggcagc tgttttttct ccagtgatta
      721 aaccaatatg aagaggaaga gaaggaaacg ggagctcagc atttttgcgt aatagtcctt
      781 cggttcttaa tttttttagg atggcttctc ttgtaatagc gagttcccct aatgtataag
      841 cgggatcaat atcaatcact ttagattgaa atttcccata aggaatataa aagtcagctt
      901 gaataagtaa attgacttta agttgttctt tgagttcaaa aggggtaggg agagatgcta
      961 gtttcttttt taataaagag taatcagact taaaaatata aagaggcaac gtggcgacgg
     1021 gcttcacatc atctttaaca aattcagcaa gatttagata aatcatgttt cctcgttcag
     1081 tcatttgggt gagaacccca tgaatccaaa cagaaggcac ttgatttgtg atcaaatttt
     1141 ttattgaagt aaggtatttg ctaactgggt atgattttat ctcttcttgc ataaaggtaa
     1201 attaaatttc atcgtgaatc aatacaagtg attttagaga ttattatcgc attttagaca
     1261 tctccaccaa aaagtaggca aaataaggac ttgcatttta ctattgatta tttaatacaa
     1321 tcaatagtta ttataattta gtgagatcga tctttgacgg agaacatatg agaataaaac
     1381 cacttttaac agcatttttt ctgatgatgt ttatgggatg cgcttcaaat caagctcttg
     1441 taaactcatc tattcaacaa gcagagtcct taaaaacaac cgcaggcgaa agtaaatctt
     1501 tgtctgttaa aacgactctt gctgatgaaa aattagagga agcaaaaaaa caaaatgaaa
     1561 tagggaaaac agaagaggct ttactcgctg cagaagaaag ccttctacaa tatcgtttag
     1621 ccttagctca aagtacacat gatgctttaa gtaaagatta tgccgctctc gaaaaagagg
     1681 ttcaatcagc tgaatcttat aaaaatcaat taaaatctaa attagaacag atgaggaagg
     1741 aaaaatgaaa ttagcaactc ttttttacac gtctctttta agtgcttcct ttatttttgc
     1801 ttctaattcg aatacggatt cgctttctac aaaacaagct gcttcgtggt ctgtgaaggc
     1861 ttgtcaggaa tctttaacta acttaaataa agaaattcca gctgaagctt tagatgcaaa
     1921 gattacttta gctgaagctc aagcaaaagc atctctagtg gccgcgcaag aaataaaaaa
     1981 tccaaaagct acttctgtgg ctgcctttca aaagacctgc tccttgtatg tggatttaat
     2041 tcaggttcaa gcaaaaacac aactcttgag aaactctctt caatctctct gggaaaaacg
     2101 tacttctaca atgcagaccg ctatggctac aaaagatagt attgaaaatt tctataaaaa
     2161 taaaacctct gcttttgcaa atgctttaga agaagaaaaa gaacgtctcg ctttaacaag
     2221 tgaagaactt atacaaaaag caaaagctcg cgaagattct attaaagccg ctgcagacga
     2281 aagagaagct gctcttcaaa aggctttaga cgaagaacgt caaaaggcag aggctcgtca
     2341 acaagaagca aaaaataaat taaatgagtt acaatccaag cttattcaag taactcaaga
     2401 tgctcgtggc attattctat ctatgtcaga tattttattt gacgtgaata aagcttcttt
     2461 gaaaggcgac ttgaaaacta gcttagctaa ggttgctgga attttgtctg tatatcaaga
     2521 actcaatgtt tctgtagaag ggcatacgga taatacgggt tcagaagaac acaacttaaa
     2581 actttctgaa caacgcgcac aaaatgtact tgacttctta gtgaatcaag ggattgaagc
     2641 ttctcgtttg acttctaaag gctacggaat gactcttcct attgcaaata ataagactaa
     2701 agaaggacgt caaaaaaata gacgtgtgga tttagtgatt aaagataagg ctctgtaaat
     2761 ctatggccaa gattgtttca gcaatggaag tgcgtcagaa ttttggatcc cttttaaatc
     2821 aggtttctct tcttggagaa gaaattgtga ttgaaagatc tgggaaatcc attgctcgat
     2881 tagtctctat ttctgaacca tctttatcaa aactggattt cagagatatt gcgaagttac
     2941 caagtattaa ataaaaatat tttacttata aaaaaagacc tgaaggcatt accttcaggt
     3001 ctttttaatt ccatgagaat tacatcgcac cacgttcaag cttgattgtg aagttagtca
     3061 catcacaaac ttcgcttggg ccccaatatt ggatagggcc tggataggta taagattctg
     3121 tcttagccca ttcatcacga cgagctgcaa aatacttaaa tggagcacca tctagttcaa
     3181 caagagcttt ttgaatcaca ggtttatctg ctccatgacg acgttcaata ttcatcatca
     3241 tggttatagg aatgccacca gcagtccagt tctctattcc tttagttagg ttgcgaacag
     3301 agctcatgta accattcatt ttattgaggg caagtacaga tgctgcataa ccaaggctat
     3361 agcagtaatc ggcatcaaaa ttggatgggg ctgcacaacg tccttcgtag ccaaagaagt
     3421 ggttgagagc agcaaacttt cctttgaagt cagaacgttt tttaagttct ttagcaacca
     3481 tttcaataat tagtttttca gtttcgatca aagaaacttg aacgttaccg tgagaatcac
     3541 gatcaagcat taattgagct tgaatgccag ctgggagact ttttaacacg ttagcagatt
     3601 ctgtgtttaa ccaaccacaa agtttttcca ctttgtcttg agtgtttaaa ccttctactt
     3661 ctttttcgtg gtgagcaaga acttcagaaa gttcagaaat taatacgcct acatcaggaa
     3721 taaattcgag taagccttca ggaatcaaag cgacaccgaa attcttacct tggctagcac
     3781 gttccgcaac aatgtcagcc acatatttta ccacttctga aagcttcatt tgttttgctt
     3841 ttacttcttc agaaataagg caaatatttg gatgagtttg tagagctgct tctaaaccaa
     3901 tatgagaagc ggagcgaccc atgagcttaa taaaatgcca atacttttgt gcggagtttg
     3961 catcacgcat gatattaccg atgagttcgg aataggtctt tacagcggta tcaaaaccga
     4021 aagaagtttc aatatattca ttctttaagt caccatcaat tgtttttggg caaccgcaaa
     4081 caacgcagga tgcgcctttt gcttggaagt attcacctaa aacagcagcg ttagtattgg
     4141 agtcatcacc tccgataatg gttatggcat ctaattttaa atcattagcg actttaatgc
     4201 aacgatcaaa ttgttctgtt gtttcaagct tggtacggcc tgattgaata atatcaaatc
     4261 caccagtatt gcgataagcg tccattaatt tggcgtcaat ttgaatatat tttccgtttt
     4321 ctaaaccaga aggtccacca agaaaaccga agagtacaga gtttttagag atggattgaa
     4381 gaccatcaaa aataccagca atcacgttat ggccacctgg agcttgacca ccagaaagaa
     4441 caacgcctac acgaatcgct tttgattcgc aagtgcaaga gcaatcacag gcaggtgtaa
     4501 atgaaacaac tggagctcca tatgtattag ggaacaaagc cttgattttt tcttggtctc
     4561 gaacggactc tgtggcagtt cctttattaa tagaaacctt caaagcacca tttttcaaag
     4621 aagagggaag tttgggttgg taagctgcgc gagctttacc cagaacagat agattatcag
     4681 acatagtaac ctctttaggg gtgagttaaa ttttaccgct taaagttaaa aaaaaaagtc
     4741 taaaagaaaa aagaaattgt tcctctggtc ttggttaaat aggtattctt ctcgtatttt
     4801 tgtattttta gagctatgaa aagattacaa tgcttaacaa ttattttatt cgttctctct
     4861 agcttccctc tttgggctca aaataaagac atacgctttg tcccagagtc ttattctgta
     4921 ggtgttatag agcaaggaga tgttcgtcat atcgttttaa aaggcgtaaa taccacctcg
     4981 aaaacgattg ttctagaatc tgtcatgagt cagaatacag gctcatctaa tttcaagcac
     5041 ccgtctgtga ttaaaccaaa tgaaacgatt acgattgagt ttgatttaaa tacatcgtat
     5101 atggaaggtg cttttaatca tagcatcgtt cttgtggata caacaggggc tttctataca
     5161 acgacaattg aaggtgacgt tcagtctcca gtatttttta gtgaaaaaat gtttgatgca
     5221 gggtattaca aagcccgaga aaaacgcgag tggacttttt atgtttggag tacggataaa
     5281 aaaactcgcc cagatataga actagattct atcgcggcaa aatctttttc tgcaacgtat
     5341 aaatctgtga tgctgaacat tgataaattg gatcagatta aagaaggtgg aaaagtacct
     5401 gctcttaaag taaccctaaa aacaaaagga attccaagag atgggatcgc cttaaaacaa
     5461 aagagtttac gccatatcgt ggcatttaaa agcaaggctc atccaaaagc gacacctgaa
     5521 attttaatag ttggttattg gaaataaata tctttcgagg cttgccaaat aagaaggaaa
     5581 aactaaattt atccgaaatc agaacgggat gtagctcagt ctggtagagt acttgaatgg
     5641 ggttcaagtg gtcgatggtt cgaatccatt catcccgata aaaaaaagac cttaaatagg
     5701 tctttttttt tacttgttac tctttagaag aagtttcttt atcatctatt tcttcaattt
     5761 cgtcatcgtc ttcattttgt tgatccattt caaaatcact aggatcattt ttttcttcgt
     5821 tatcaattgg acaagaagcg tataaaaaag ccttttggtc taataaagcc attttatatt
     5881 catcttcaga cgtgtcttta ggatcccaaa ttacgacttg gtttcttccg ttcattttcc
     5941 ctttataaag agcgtggtct gaaagttgaa tacttttatc aacgcccagt ttttgatcat
     6001 agagggcaac acctaaggtg attgtaacgc caataattgt atcagaaaac tcaattttca
     6061 tctgagctac ttttcttcta aaacgctcag cgactatttt tgctccatca agatctgttt
     6121 ctggtaaaac ggttaaaaat tcttcaccac cccaacgaga tacatagtcg tatttcctta
     6181 agttttttct gataatatca gcaacgcctt ttagtactaa gtctccacaa tagtgaccgt
     6241 aggtatcgtt aatgtctttg aatttatcaa tatcaataaa tataaagcaa aaatctctgt
     6301 gagttctttc tgctcgattt gcttcttgtt ggatcttttc acggatatca cggcgatttg
     6361 gaagttttgt tagttcatct gttcttgaaa ttaaatcaag ttgatttttt gcttcttcta
     6421 attcacgagt gcgttctaaa acttctaatt ccaaatgctc tttatgcaaa tttaagtctt
     6481 gaatttgctt ttgtataatc gattgcatct ggttaaagga ctctgttaaa atacctaatt
     6541 cattttcctt attgatttcg ggtaagggct cggaatcgaa atttccactg gcgatataat
     6601 taatatgttc aataatatta cgtaatggaa aagcaaagtt cttaaacagc catagaataa
     6661 ttaaaatggt agcaacaatt gtgagggcta acaaaaaaac cattgtatag gcaacagcag
     6721 cgattaaaga ttgaacttcg tccctcgatt gtaatgaaag aattccaatc tgatagcgtg
     6781 gattatagct ataggcagcc aagtattgtt tgttatttag agtgatgaaa gtaggcttag
     6841 taatggaaaa tgttttagct gaaaaatctt ttaagcctaa atcagaggca taaagttctt
     6901 gctctaagat ttttgataat tctgggtata gtagaaccct attcttgtcg tcaaaggcga
     6961 ttaaaaaacc atcttctcca attttatgag aagcatattt ttgccaaatt cgtttaaatg
     7021 aa
//
LOCUS       DAOOBB010000092         6978 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_12876_length_6978_cov_5.159902, whole genome shotgun sequence.
ACCESSION   DAOOBB010000092 DAOOBB010000000
VERSION     DAOOBB010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 6978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6978
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12876_length_6978_cov_5.159902"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(343..1527)
                     /gene="rmuC"
                     /locus_tag="PLT31_05430"
     CDS             complement(343..1527)
                     /gene="rmuC"
                     /locus_tag="PLT31_05430"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HPW94616.1"
                     /translation="MFLAFLIINTVLLLAVILLLFFKSSEKENFWKEFQTASMQLTSA
                     LDQSSRANREEQNRSIERLTFEMKNSIDKMRETMEARFQNIQKENAEKLEQMRLTVDE
                     KLHNTLETRLGESFKVVSERLEQVQKGLVEMQTLASGVGDLKRVLSNVKVKGVLGEYQ
                     LASILEQILTPAQYAQNVKTKKGSANIVEFAVKIPSKSDSMNDIWLPIDAKFPSEDYE
                     RLMSAYDLADVNAIEEHKKKLESKIKVFAKDIKDKYIDPPQTTDFAILFLPFEGLYAE
                     VLRIPGLFEQVQRDYKITIAGPTTITAFLSSLQMGFRSLAVEKRTSEIWELLGAVRTE
                     FSKFGVALSNAKNKLDLASKEIDNAGVRSRAIERQLKNVQTLPETKAKMILGDDLTTA
                     LD"
     gene            complement(1744..3000)
                     /locus_tag="PLT31_05435"
     CDS             complement(1744..3000)
                     /locus_tag="PLT31_05435"
                     /inference="COORDINATES: protein motif:HMM:NF002759.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HPW94617.1"
                     /translation="MKYLDYLNGSITPYHAVDITAKKLLENGFQELSFQSKWSLEPSK
                     AYFIKWGYTTLFAFRTPKQVNSSIFAKLALAHTDFPALKVTPNACRFESGVQLVHSEI
                     YGSPIFYSWLDRDLGCGGVLVFEKDNQIQHRLVQASQKFRIPELSIHLQRGVNQEGRK
                     VDAQKGLDVFWSSSEKDSLDSWVKQQLSPEEKLLDYDLRFYDAELASLGGFHEEWVYS
                     GRIDNLASCHAALEALLATDSAENYISGFCLFNHEEIGSETKEGAQSLFLKMTLDQII
                     KALNISEQCTPSFLEKSFFVSMDMAHALNPVYPEKHDPKHAPILGQGIVLKQNAQMRY
                     ATDVFSSAVFKQICSKAGVSYQNFISRNNLPCGSTIGPSVSSTLGVSTVDIGEPMLSM
                     HSIREMSSVQDHQQMIKFLASLFKDF"
     gene            complement(3008..4336)
                     /gene="murD"
                     /locus_tag="PLT31_05440"
     CDS             complement(3008..4336)
                     /gene="murD"
                     /locus_tag="PLT31_05440"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HPW94618.1"
                     /translation="MQSVVAPVGILGFGVEGQSSLQYLLSIGIKEIVIMDKNPIDPQL
                     LPKETEIKTYTGEQYLDGLKECVTVIRSAGVYPMLPELLKFQMNGGLLTSQIEIYFQE
                     TKAKKIIGVTGTLGKGSVVSMIAHILKSVDKPCEIGGNFGIPALDLLKTDSPDRVVIL
                     ELSSFQLMTLSISPSIAVVLRVTSEHLDWHRSVEEYRDAKANLVRYQKKENCCIYLQD
                     SVHSTAIAKQTKALTLSFGHGACDACVEGETLSVSGHKLNLSECKIRGVYQLENMAAA
                     ALACKALGVFGGESLQALKSYEPLRFRMQYKGEKAGIEFYNDSYATRPDATIAAAKSM
                     KRPFSLILGGSEKNADFMELASFLTSLPLLKRVAFIGDTANRLEQLLLEAGVSVPMNI
                     FPSLDAAFADALQIGEGGALLFSPACASFGLFKNYKARGEAFDHLVDLLS"
     gene            complement(4475..5203)
                     /locus_tag="PLT31_05445"
     CDS             complement(4475..5203)
                     /locus_tag="PLT31_05445"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPW94619.1"
                     /translation="MNIQGKWALITGASRGVGREIARAMASLGSNLILQSRALEHMAP
                     LIEELKSKGVKLKAVACDLESEKSIQAMLQEIDSMGVIVDFVFNNAGLQVPYCPDFYS
                     NKREDYVQSFAVNCLAPVQIAYHFLPKMVANQFGRIVMTTSGIADQPELMGYACAKAA
                     LTKFVADFACKFNGTNVMMNVMDPGWLRTDLGGPNAPNSVESVIPGALVGALLDDQKS
                     GRWFNAQDYAGLSIEEALLKAKNK"
     gene            5532..5843
                     /gene="trxA"
                     /locus_tag="PLT31_05450"
     CDS             5532..5843
                     /gene="trxA"
                     /locus_tag="PLT31_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005344840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPW94620.1"
                     /translation="MNVIEITKENFEQEVMKSTKPVLIDFWASWCGPCRMIGPIVEEV
                     AKEVTHAKVGKVNVDDQPELAQQFQISNIPTLVVMKDGKIVNKVVGGRPKADLVEMLN
                     V"
     gene            complement(5833..6525)
                     /locus_tag="PLT31_05455"
     CDS             complement(5833..6525)
                     /locus_tag="PLT31_05455"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPW94621.1"
                     /translation="MDLEYKPFLEKAFSFWTHLLPSEQALILEKMQVLKYPKGALVHS
                     AGNDCLGMLLIKSGELRVYMVSDEGREITLYRLVEGDVCVLSASCVLSSITFDVYIDA
                     EVDTVLFQMPSNVFADLAEKNIYVESFGYQISAQRFSDVMWAMQQILFMSFDQRLAIF
                     LTDESIKTGSDTLLLTHEQVAKYMGSAREVVTRMLKYFAAEKIVALSRGAIQIVDKKK
                     LRSLTERSHIKH"
     gene            complement(6571..>6978)
                     /locus_tag="PLT31_05460"
     CDS             complement(6571..>6978)
                     /locus_tag="PLT31_05460"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HPW94622.1"
                     /translation="FSMADSYKDCYSWGVSLATTPKRLEKATGLIQKEIDLFLKEGFF
                     PGELERTKQNIIGAMKINADSTDKRTLRLAEQQLHLGHYCSMQEIEDGINSVSKEQVI
                     EMTRLVLKNDFWTAAVVQPATAKTPTALNEIRF"
BASE COUNT         2257 a         1392 c         1112 g         2217 t
ORIGIN      
        1 aaaacaatct attaacaaat aaaccccttt tatgcgtttt tatgcccttt ttgatcccca
       61 tatatatatt ggataatatt tcttatgtat aaacagcaca ttatattctc ccctttatat
      121 gtatataaaa cactttttga aaccattatt agtaatccac tccttttttt aactccctga
      181 atagaataca aaagaataat ctctctaacg ttaccataca atcatttttt tattgtgtaa
      241 ataaattcat aatgagttca aattaatgtt acaatcaaaa acattattat cacgttaaat
      301 acatacaaat gttttgacca tgaatagctt gttctgaatt aatcaatcaa gagccgttgt
      361 caaatcatca ccaagaatca ttttagcttt tgtttctggt aatgtttgaa cattttttaa
      421 ttgacgttca atcgctcgag aacgaacacc tgcattgtca atttctttac tagccaaatc
      481 caatttattt ttcgcattag aaagcgctac gccaaattta ctaaactccg tacgcaccgc
      541 tcccagtaat tcccagattt cgcttgtacg cttttctaca gcaagacttc taaaacccat
      601 ttgtaagcta gataaaaaag ccgtgattgt tgttgggccc gctatagtga ttttataatc
      661 tcgttgtact tgttcaaata agccagggat acgcaacacc tcagcataaa gcccctcgaa
      721 tggtaaaaat agaatagcaa aatcagtagt ctgaggagga tcaatatatt tgtctttaat
      781 atcttttgca aagactttta ttttactctc taattttttc ttatgttctt caatagcatt
      841 tacatcggca agatcatatg cactcattaa tcgttcgtaa tcttctgaag ggaacttggc
      901 gtcgataggg agccatatat cattcataga atcacttttg ctcggaattt taaccgcaaa
      961 ttcaacgatg tttgcgcttc cctttttggt cttcacgttt tgagcatatt gtgcaggcgt
     1021 taatatttgt tctaaaatag aagctaattg atattctcct aaaacaccct tgactttcac
     1081 attcgataag actcgtttaa gatcccctac accagaggca agtgtttgca tttctactaa
     1141 gcccttttgc acttgttcta aacgctcgct gactacctta aacgattccc ctaatcgtgt
     1201 ttccaatgta ttgtgtaatt tttcatctac cgttaaacgc atttgttcaa gcttttctgc
     1261 attctccttt tgaatatttt gaaacctcgc ttccatcgtt tctctcattt tatctatcga
     1321 gtttttcatt tcaaaagtca atcgttcaat agaacgattt tgttcttctc gattggctcg
     1381 cgaactttga tctaaagcag atgtcaattg cattgaagcc gtttgaaatt ctttccaaaa
     1441 attttctttt tcagatgatt tgaaaaataa aagaagaata actgcaagta ataatacggt
     1501 atttatgatt aagaaagcaa gaaacatcat tcctcctaaa aaagaatcaa aggactaaaa
     1561 ctcatttaaa actacatcta taatactctt atacaaaaga ttatcacaag ctctttttaa
     1621 ctattcctta aaacagtttt ctatttaatc ttaattgtta aaaaatttaa caacctattc
     1681 ttaaagccac tgatttaaca gttctataaa aatactctat taaattgaca ttttttgtct
     1741 ttcttaaaaa tctttaaata aagaggccaa gaactttatc atttgttgat gatcttgaac
     1801 agaagacatt tctcgaatac tgtgcatact aagcattggt tctccaatat ctaccgtact
     1861 tacgcctaga gtgcttgaaa cagatgggcc tattgtactc ccgcaaggca aattatttcg
     1921 actgataaaa ttctgataag aaacgccagc tttcgaacat atctgcttga aaacagctga
     1981 agagaaaaca tctgtggcat aacgcatctg cgcattttgt tttagtacaa taccttggcc
     2041 taaaatggga gcgtgtttag ggtcatgttt ttctggataa acgggattta atgcatgagc
     2101 catatccata gaaacaaaga aggatttttc taaaaaagaa ggcgtacact gctctgaaat
     2161 atttaaggct tttattattt gatctaaagt cattttcaag aatagacttt gagcaccctc
     2221 ttttgtctct gaccctattt cttcatggtt aaaaaggcaa aatccagaaa tgtaattttc
     2281 agcggaatct gttgccagta aggcttcaag tgccgcatgg caagaagcca aattatcgat
     2341 acgacctgaa tagacccact cttcatggaa acctcctaaa gaggctagct ccgcatcata
     2401 aaagcgtaaa tcataatcta acagcttttc ttctgggctt aattgctgtt taacccaaga
     2461 atctaaagaa tccttttcac tagaagacca aaaaacatct aatccttttt gagcatctac
     2521 ctttctacct tcttgattga ctcctcgttg caagtggatt gaaagctcag gaattctaaa
     2581 tttttgagaa gcctgcacca aacgatgttg aatttgattg tctttttcaa aaacgagaac
     2641 accgccacaa ccaagatctc gatcaagcca agaataaaaa ataggtgatc cataaatttc
     2701 tgaatgaact aactgaacac ctgattcaaa gcggcaagca ttaggcgtta cttttaatgc
     2761 aggaaaatct gtgtgagcta aggcgagctt ggcgaagata gatgaattga cttgcttagg
     2821 ggttctaaaa gcaaataaag tggtataccc ccatttaatg aaataggctt tagaaggttc
     2881 taaggaccat ttgctttgaa aagagagttc ttgaaaccca ttttctaaaa gtttttttgc
     2941 agtaatgtct actgcgtgat aaggagtgat agaaccattt aagtagtcaa gatatttcat
     3001 ataaaactca tgataaaaga tcaactagat gatcaaacgc ttcacctcta gctttgtaat
     3061 tcttaaataa tccaaaagag gcacaggctg gagaaaaaag taacgcgccc ccttctccaa
     3121 tttgaagagc atcggcaaaa gctgcgtcta gactagggaa tatgttcata ggcacagaga
     3181 ctccggcctc taacaataat tgttctaaac ggtttgctgt atcaccaata aaagcaactc
     3241 tttttaatag aggtaatgaa gttaaaaaag acgctaactc cataaaatca gcattctttt
     3301 cagaaccgcc taaaatgaga gagaaaggtc gtttcatgct ttttgctgca gcaatagtgg
     3361 cgtctgggcg agtggcataa gaatcattat aaaattcgat gcctgctttt tcacctttat
     3421 attgcattct aaaacgaagc ggttcatacg attttaatgc ttgtaaagat tctccaccaa
     3481 aaacacctag agccttacag gctaaagcag cagcagccat attttctaat tgataaacgc
     3541 ctcgaatttt gcattctgaa agattgagtt tatggccaga aacagaaaga gtttctcctt
     3601 caacacaagc atcgcaagcc ccatgaccaa aacttaaagt aagagccttt gtttgttttg
     3661 caatggcggt ggaatgcacc gaatcttgta aataaataca acaattttct ttcttttgat
     3721 atcgaactag atttgcttta gcatctcgat attcttctac actacgatgc caatctaaat
     3781 gttccgaagt cactcttaaa acaacagcga tggaaggaga gatagagagt gtcattaatt
     3841 ggaaagaaga taattctaaa atcaccactc gatcgggaga gtctgttttt aacaagtcta
     3901 aagcagggat tccaaaattg ccaccaattt cacaaggttt atcgactgat ttcagaatat
     3961 gagcaatcat gctcaccaca cttccttttc ctagtgttcc tgtcacacca attatttttt
     4021 ttgcttttgt ttcttgaaaa taaatttcga tttgactcgt taataagcca ccattcatct
     4081 gaaacttcag taattcaggt aacataggat agactccagc ggaccgaatc acagtcacac
     4141 attctttgag tccatctaag tattgctctc ccgtataggt ttttatttcc gtctctttag
     4201 gaagtaattg tggatcaatt ggatttttat ccataatcac aatttctttt atgcctatgg
     4261 agagaagata ttgaagagaa ctttgacctt caacaccaaa acctaaaata ccaacaggag
     4321 caacaacact ttgcataatg atccttctaa taactacaat ggaatataaa aaagcatccc
     4381 atttagagat gcttaaaata tataaaagcc tcatttaatc tttttattcc aaatagaaaa
     4441 tagcccttat gagatataat tacgccaata acagttattt gttttttgct tttaataaag
     4501 cttcttctat tgaaaggccc gcataatctt gagcattaaa ccaacggcca cttttttgat
     4561 cgtctaaaag ggctcctact aaagcaccag gaattaccga ttcaacagaa tttggtgcat
     4621 tcggtccacc caaatcggtt cttaaccatc ctggatccat gacattcatc atgacattag
     4681 tcccattgaa tttgcaagca aaatccgcta caaattttgt taaagcggcc ttagcacaag
     4741 cataacccat caattcaggc tggtcagcaa ttccacttgt agtcatcaca atgcgtccaa
     4801 attgatttgc caccatcttt ggcaaaaagt gataagcaat ctgcactgga gcaagacaat
     4861 tgacggcaaa agactgaaca taatcttctc ttttattgct gtaaaaatct ggacaataag
     4921 gaacttgtaa gccagcatta ttaaagacaa aatcaacgat aacacccata gaatcgattt
     4981 cttgaagcat tgcttgaatt gatttttctg attctaaatc acaggcaaca gcctttaatt
     5041 tcacgccttt agatttgagt tcttctatta atggagccat atgctccaaa gcacgacttt
     5101 gtaaaatcaa attggaaccc aatgaagcca ttgcgcgagc gatttctcgg cccactcctc
     5161 gagaagcgcc tgtaattaat gcccattttc cttgaatatt cataaataat ccttttgttt
     5221 ttcataaaat attttttttg ttcgcttttt gacgttcttt ttccattttt ctgtttacaa
     5281 attaaaacag ccccttttta gagccgtttc tttcatttga aattctctaa acaggctttg
     5341 catcaatgca ataacgaata cttcgtcaag attacttgtt cttttttaag aagatctcta
     5401 ctttggtaac ttgatcactg aaccacgagt ttgaataaaa tacattgtat ctataaaaca
     5461 atgaataaga ccctctctaa aaattgtttt gaaagcaaac ttttagagaa tgtcataaag
     5521 gagaatgatc aatgaatgtc atagaaataa cgaaagaaaa ctttgagcaa gaagtgatga
     5581 aatcaactaa acctgttctt atcgattttt gggctagctg gtgtgggcct tgccgtatga
     5641 tcggccccat tgtagaagaa gttgcaaaag aagtgacaca tgccaaagtt ggcaaagtca
     5701 atgtggacga tcaaccagag ttagctcaac agtttcaaat ttccaacatc cctacgctag
     5761 tcgtcatgaa agacggaaaa atagttaata aagtagttgg tggaagacca aaagccgatt
     5821 tggttgaaat gctcaatgtt taatatgact gcgttccgtc aaggagcgca gtttcttttt
     5881 atccacaatt tgaatagcac ctctcgaaag agccactatc ttttcagctg caaaatactt
     5941 aagcattcgt gtgaccactt cacgagcact tcccatatat tttgcaacct gctcatgcgt
     6001 gagtaaaagc gtatctgagc cagtcttaat agattcatcg gtcaaaaaaa tcgcaagcct
     6061 ttgatcaaaa ctcataaata gaatttgttg catagcccac atgacatcgg agaaacgttg
     6121 ggcagatatt tgataaccaa aagactccac atagatattc ttttctgcga gatctgcaaa
     6181 aacattagac ggcatctgga acaaaaccgt atccacttcg gcatcaatat aaacatcaaa
     6241 cgtaatagaa cttaaaacac aagaggctga aagaacgcag acatcgcctt ccactaatcg
     6301 gtagagagtg atttctcttc cttcgtctga aaccatataa acacgtaatt caccgctttt
     6361 tattaaaagc atgcctaagc aatcattgcc tgcactatgt actaatgccc cctttggata
     6421 ttttagaact tgcatttttt ctaaaatcaa tgcttgttca gaaggcaaaa ggtgcgtcca
     6481 aaacgaaaaa gctttttcta aaaaaggctt atactccaga tccataaaag actccctgtg
     6541 tttgttcagg caaaagactt cataatttta ttaaaaccgt atttcattta atgcagtagg
     6601 tgtttttgca gttgcaggct gaacaacagc tgccgtccaa aaatcatttt taagaactaa
     6661 gcgcgtcatt tctatgacct gttctttaga aacagaatta attccatctt ctatttcttg
     6721 catagaacaa taatggccta aatgcaattg ttgttcggct aatcttaagg ttctcttatc
     6781 agtgctatct gcattaattt tcatagcccc aataatattc tgttttgttc tttccaattc
     6841 tcctggaaaa aagccttctt ttaagaatag atctatttct ttttgaatta agcccgtcgc
     6901 tttttctaat cttttaggag tggtggctaa agaaacgccc cagctataac aatctttata
     6961 agaatccgcc atcgaaaa
//
LOCUS       DAOOBB010000093         6964 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_12892_length_6964_cov_4.295846, whole genome shotgun sequence.
ACCESSION   DAOOBB010000093 DAOOBB010000000
VERSION     DAOOBB010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 6964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6964
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12892_length_6964_cov_4.295846"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PLT31_05465"
     CDS             complement(<1..396)
                     /locus_tag="PLT31_05465"
                     /inference="COORDINATES: protein motif:HMM:NF013589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HPW94623.1"
                     /translation="MSKTDRIIRATGKKTPMRLVLVDITETMNFIGQKHNASGYSLKL
                     LAEASIGSLFLSSSLKYQGTVCLKVLFSGDYSFVQADTTPQGLIRAMIPQNEIQKNKH
                     FEPALLHQSVEVIKLNDKGKRLQESVVEAA"
     gene            500..1054
                     /locus_tag="PLT31_05470"
     CDS             500..1054
                     /locus_tag="PLT31_05470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HPW94624.1"
                     /translation="MHKILFLMADGFEEIEFTTVYDILIRGGVKVTLAGIYNDARVIG
                     AHGLSIRADAVLSELYLGNYQGVFLPGGSTGVENLSESDEVIECVQKFFTDEKWVTAI
                     CAAPKVLAQAKVISNYTVTSFPSVREEVEPHCKSYSEERVVRDRNIITSRAAGTAEEF
                     ALEFLKVLEGESVSEKIRSQILAR"
     gene            complement(1540..1613)
                     /locus_tag="PLT31_05475"
     tRNA            complement(1540..1613)
                     /locus_tag="PLT31_05475"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Met,seq:cat)
     gene            complement(1720..2883)
                     /gene="pgsA"
                     /locus_tag="PLT31_05480"
     CDS             complement(1720..2883)
                     /gene="pgsA"
                     /locus_tag="PLT31_05480"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HPW94625.1"
                     /translation="MNNEMSPSQILRLRGRIWSILRALLFVLVIYFIWSEKTFFAATC
                     VLAALIMGWINTYQLHVQEIEKPYYRVWLNFIDGLLSFAVMTSILIRNWIKNDEMEMI
                     LAVGCVFLLIRLISYTLFSVGVLREGKQIARRGSWSKLATIAITLTMGVYLLNLEHYM
                     QIMMVASLLLMGASSASFLYWYYRDPAHRKPLSVASQLTMSRIVLTPFLLWVFFYDND
                     LNYSNNNITFKILALIMVLGFMLTDFLDGYLARKMGDVSTLGKYLDPFSDKIANMTIF
                     LCFIATDYAPVWMVALIYFREASVETLRTLAASEGIVMPARKSGKWKTALQGIGIVVI
                     LLGALDPISQIIPNYEQIWNYLPNTVMGIITAITLISGVDYFVASKHIIKKFL"
     gene            complement(2880..4532)
                     /gene="recN"
                     /locus_tag="PLT31_05485"
     CDS             complement(2880..4532)
                     /gene="recN"
                     /locus_tag="PLT31_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HPW94626.1"
                     /translation="MLKQLSIRDFTLIAQCDISFGPGFTAITGETGAGKSVLLKAIRL
                     ICGEKSTATMVRAQAEKATIEATFDISKNDPLKKILDSLEIDYDDELIIQREITESGK
                     SRARVNGSVINLNDLQTLGESLIQMHGQSEQILLRDVRTHGQMLDDFCSNQPLLEKYT
                     SLFSEWNQKKAQMEALKERAQNLAAQKDFLKFQFEELSKAHLKAGEEEELESLTSQAS
                     KGETERRFLEDMNRILESENGLRDQLRSFSAKMKQLALKIPRYGESLVLFQEVLDPLE
                     SFLKDFSSISPATEVSPAELDKANARLALIQKLKRKYRTDIDGLIALTNTRKEELESL
                     DNLDADLEEISHQIKKIEIELNDVAQTLSEKRIAGATKLDTSVESILHELGMPGARFK
                     TSILRTVLHATGFDKVEFMLAPNTGEGEKSLQKAVSGGELSRVLLAFKSVMAELDTTP
                     LLIFDEVDSGISGEIGNQIGVALQKLGKHHQVLTITHLHQVASRAKGQLAVKKEEIDK
                     RTYTHIFNLDYDSRILELSRMLGDEKSKTVQEHAKQLLEVQL"
     gene            complement(4547..5284)
                     /gene="trmB"
                     /locus_tag="PLT31_05490"
     CDS             complement(4547..5284)
                     /gene="trmB"
                     /locus_tag="PLT31_05490"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HPW94627.1"
                     /translation="MEKMKEEIENQKEIPEFYRDLKEDPDEKGLWYFFFRNNPEARRK
                     PIQTPDNKPHKIDFQWNELFSDASKPIEIEIGSGKGGFLVEYAQKHPELSLLGSEWDK
                     TWAVFCMERLKKKKVLDHTQMLCGDMFYFLRDAVPSNSVDAFHMYFPDPWPKERHHKN
                     RLLRPDFLTEVARTLKPGKKLFYWGTDHKEYNEVALETFDQFDGCTIIERNTASPTEG
                     IMTGFEKKYRKEGRPIYRSLIVFEKEI"
     gene            complement(5358..6176)
                     /locus_tag="PLT31_05495"
     CDS             complement(5358..6176)
                     /locus_tag="PLT31_05495"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPW94628.1"
                     /translation="MTTENKYIHNALKQTHLQTKRFNLSGFSISGLASYHLLPELNFA
                     VDMGECPLTAIPIDHVFLTHAHGDHSRCLMRHHSLRKMMGLQKDAAYYLSEKIYEKAC
                     QWIRAEAAFEGVPEYRIQIPNLIPIKTDEKIILQYRKDLALEAFEVSHSVPTLGCTLF
                     RFKKKLLEKYLKCTSQEIIDLRQNHVEVTQDIYEPIVTFMGDCKSESLLDNASIWKSP
                     VLVTECTFIDDHEEEMAQKKGHSHLNRIISIFEEKKDEMECEKIILNHFSMKYS"
     gene            6351..>6964
                     /locus_tag="PLT31_05500"
     CDS             6351..>6964
                     /locus_tag="PLT31_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HPW94629.1"
                     /translation="MNRYDLVLLGFILERECSGYDIMTEIRKRELDRWAKISTSTIYN
                     RLIRLEKNEYITGRSERDGNRPERMMYTITDKGRELLKKEILKHLTGFNDDPRTLGYA
                     FLYGVEPKEAVRSLEVHERRLLQEIERLNEMIANEPRPTLFSEGPFLNCMSRDHILVE
                     LKYVRAAISILRDPSRNKQLDGYFYINFGARHFDKREPSTDSNR"
BASE COUNT         2070 a         1239 c         1342 g         2313 t
ORIGIN      
        1 agcggcttct actacgcttt cttgaagacg tttgccttta tcattcaact taatcacttc
       61 tacgctttgg tgtagaagtg ctggttcaaa atgcttattt ttttgaattt cattttgtgg
      121 aatcatggct cgaataaggc cttgcggcgt tgtatcggct tgcacaaaag aataatcccc
      181 agaaaaaaga actttcaaac aaactgttcc ttggtatttt aaggaactag agagaaaaag
      241 agatccgatg gaggcttcgg caagtaattt taaagaataa ccagaggcgt tatgcttttg
      301 tccaataaaa ttcattgttt cagtaatatc cactaaaaca aggcgcatgg gtgttttttt
      361 gccagtagct cgaataatgc gatcagtttt actcatagtg caaatataag aaaaaagaag
      421 attttttcta ttttcacatt acatttagat aggattataa gtattttttt aacatagttt
      481 tgttttttag aggcttaaaa tgcataaaat tttatttcta atggcagatg gttttgagga
      541 aattgaattt acgactgttt atgatatttt aattcgcggt ggagttaagg tgactcttgc
      601 tggtatttat aatgatgccc gtgtgattgg ggctcatggt ctttccattc gtgccgatgc
      661 tgttctatca gaactatatc tcggaaacta ccaaggagtc tttttacctg gaggtagtac
      721 gggtgtagaa aacctttctg aaagtgacga ggtcattgaa tgcgttcaga aattttttac
      781 agacgaaaaa tgggtgactg ctatttgtgc ggctccaaaa gttttagctc aagcaaaagt
      841 gatttcaaat tatactgtaa cgagcttccc ttcggtgcgt gaagaggtag agcctcattg
      901 taaaagttat agcgaagaac gtgttgtgag ggatcgaaat atcattacga gccgtgccgc
      961 tggtacagca gaagaattcg ctttagaatt cttaaaagtt cttgaaggcg aatcggtttc
     1021 agaaaaaatc cgctctcaga ttttagctcg ttaaatttat tccatgtttt ttgttaaggg
     1081 gctactttca agtggccctt tttttgtgca aataaaaaaa tgaaaggaat tttacttttt
     1141 gcatcttgcc aaaattattg gtgaaaagtg aaaatttatt ttaatgaaat atgaaatgga
     1201 aatcttgtat ttttatcata acgtgttttt tattcaatca tcaatgggga actctttttt
     1261 tgtgcctttc catttcatta atcttcgttg taaaatgtaa ttatcgtttt ttttattttt
     1321 ttaatcgtta tattgataat cttttttctt aaattccatc gcgaaacttt tccaagccca
     1381 gcacgaaatg gaaaatattt tttcaaataa tactctctct tttcaatttt cattgagaaa
     1441 agggagtgtt attttgcccc ttcctttttt tgattttgaa tttttgtaga aaggggttta
     1501 aaaactaaaa gagagactta aaaaagtctc tctttttagt agcgggggca ggacttgaac
     1561 gctgcgacct tcgggttatg agcccgacga gctaccaact gctccacccc gcgtcgaggt
     1621 gatacgccat atatagtaaa caggctcttt tttggcaagg ccttattttg ataaaatgaa
     1681 caataactta ttatgcgaac ttgttttttt tagaaatttc tagagaaatt ttttgatgat
     1741 gtgttttgag gccacaaagt aatcaacgcc actgatgagt gtaatcgctg tgatgattcc
     1801 catcacagta tttggtaagt aattccaaat ttgctcataa ttaggaataa tctgacttat
     1861 agggtctagt gcaccaagga gaatcactac aataccaatt ccttgcaaag ctgttttcca
     1921 tttaccgctt tttcttgcag gcatcacaat cccttctgaa gcagcaagtg ttcttaatgt
     1981 ttccacactc gcttctcgga aataaattaa tgcgaccatc caaacagggg cataatcagt
     2041 ggcaataaaa caaaggaaaa tcgtcatatt ggcaatttta tcagagaatg gatctaagta
     2101 tttaccaaga gtagaaacat cgcccatttt tctggctaag taaccgtcta aaaagtcggt
     2161 aagcataaag ccaagaacca ttattaaggc taatatctta aatgtgatat tgttgttgct
     2221 gtaattgaga tcgttatcgt aaaagaatac ccaaagtaaa aatggagtta aaacaatgcg
     2281 actcatggtg agttggcttg ctacagaaag gggctttctg tgagcggggt cgcggtaata
     2341 ccagtaaagg aaagaggctg aagaagctcc cattaataag agagaggcca ccatcatgat
     2401 ttgcatataa tgctcaaggt ttaataggta aacacccatt gtaagcgtaa tagcaatggt
     2461 ggctaattta ctccaagaac cacgccttgc aatttgtttc ccttctcgta aaacgccaac
     2521 agaaaaaaga gtataactaa taagacgaat gagtagaaat acgcacccta cagcaagaat
     2581 catctccatc tcgtcatttt taatccagtt tcgaatcaaa atactcgtca tcacagcaaa
     2641 agaaaggagg ccatcaataa aattcaacca aacacgataa taaggctttt ctatttcttg
     2701 aacatggagt tgataagtgt taatccatcc cataattaat gcagctaaaa cgcaagttgc
     2761 tgcaaaaaaa gttttttcgc tccaaataaa atagatgact aaaacgaata aaagggctct
     2821 taaaatactc caaatgcgac cacgtagtcg aagtatttgt gatggagaca tttcattatt
     2881 catagttgaa cctctaaaag ttgtttagca tgttcttgaa ccgtttttga tttttcatca
     2941 ccgagcatgc gcgataattc taaaattcgg ctatcatagt ctaaattaaa aatatgtgtg
     3001 tatgtgcgtt tgtctatttc ttcttttttg accgctaatt ggcccttggc tcggctggcg
     3061 acttgatgca agtgagtaat tgttaaaact tgatggtgtt tgccgagttt ttggagagca
     3121 actccaattt ggtttccaat ttcaccgcta attccagaat ccacttcgtc aaaaataagg
     3181 agaggggtgg tgtctaattc ggccatgacg cttttgaatg cgagaagtac tctagaaagt
     3241 tcaccgccag agacggcttt ctggagactt ttttcacctt cccctgtgtt tggggcaagc
     3301 atgaattcca ctttatcaaa gcctgtggca tggagaactg ttcttaaaat ggaagtttta
     3361 aaacgagctc ctggcattcc aagttcatga agaatcgatt ctacagaagt atcgagttta
     3421 gtcgccccag cgattctttt ttctgaaagt gtttgagcaa catcatttaa ttcaatttca
     3481 atctttttaa tctggtgtga gatttcttcc agatcagcat ctagattgtc taagctttca
     3541 agctcttctt ttcttgtatt ggtaagagcg attaagccat cgatatcggt tctatatttc
     3601 cgttttagct tttgaattag agcaagcctt gcgtttgctt tgtctagttc tgcagggctt
     3661 acttctgtcg ccggagaaat agaggagaag tcttttaaga acgattcaag tgggtctaac
     3721 acttcttgaa ataagactaa agattcacca taacgaggaa tttttaatgc gagttgtttc
     3781 attttagctg aaaacgaacg tagttgatct cgtagtccat tttcagattc taaaatgcga
     3841 ttcatgtctt caagaaagcg acgttctgtt tcgcctttag aagcctgaga cgtaagggat
     3901 tcaagctctt cttcttcgcc tgcttttagg tgtgctttag aaagttcttc aaattgaaac
     3961 tttagaaaat ctttttgagc ggcaaggttt tgagctcgtt cttttaaagc ctccatctgc
     4021 gcttttttct gattccattc actaaaaaga gaagtgtatt tttctaataa aggttgattt
     4081 gagcagaagt catcgagcat ttgcccatga gtgcgcacgt ctcgcagtag aatttgttca
     4141 ctttggccat gcatctgaat tagagattcg ccaagagttt gcaagtcatt taagttgata
     4201 acactaccat tgacgcgggc tctagattta ccagactcag ttatttctct ttgaataatt
     4261 aattcatcgt catagtcgat ttctaaggag tctagaattt tttttagagg atcattttta
     4321 gaaatatcaa aagtggcttc aatggttgct ttctctgctt gagctcgcac catcgttgct
     4381 gttgattttt caccgcagat caaacgaatg gctttaagta aaacggattt acctgcacct
     4441 gtttcgcctg taatcgctgt aaaaccaggg ccaaaagaga tatcacattg agctattaga
     4501 gtaaaatcgc gaatggagag ttgttttaac atggcgtata atttacttaa atttcttttt
     4561 caaaaacaat caaactgcga taaatgggtc ggccttcttt acgatacttt ttttcaaagc
     4621 ctgtcatgat gccttcggta ggtgaagcgg tattacgttc tataatagtg cacccgtcaa
     4681 attgatcaaa ggtttctaaa gcgacttcat tatattcttt gtggtctgtt ccccagtaaa
     4741 aaagtttttt accaggtttt aacgtacgag ccacttcagt taaaaagtca ggccttaaca
     4801 atctattttt gtggtggcgt tctttgggcc acggatcggg aaaatacatg tgaaacgcat
     4861 ctaccgagtt agagggaacg gcatcacgta gaaaataaaa catgtctcca cagagcatct
     4921 gagtatggtc taaaaccttt tttttcttta ggcgttccat acagaaaacg gcccaagttt
     4981 tatcccattc gcttcctaaa agagataatt cagggtgctt ttgtgcgtat tccacgagga
     5041 atccaccctt accagatcca atttcaattt caatgggctt gcttgcatcg ctaaacagct
     5101 cattccattg aaagtctatc ttatgaggct tattatcagg agtttgaatc ggtttacgcc
     5161 tagcttcagg gttgtttcta aagaaaaaat accacaaacc cttttcatca ggatcttctt
     5221 ttaagtcgcg gtaaaattca ggaatctctt tttggttttc gatttcttct ttcatttttt
     5281 ccatactata tcattactgt gactttgtct ttaataaatt ctggaagctt cttatctaaa
     5341 acatctagaa tgtgctttta agaatatttc attgaaaaat gattcaatat aattttttca
     5401 cattccattt catctttctt ctcttcaaaa atggaaataa ttctatttag atgagagtgg
     5461 cctttctttt gagccatttc ctcttcatga tcatcaataa aagtacattc tgtgaccagt
     5521 acaggggatt tccaaataga agcattatct aaaagacttt cgcttttgca gtcgcccata
     5581 aatgttacta taggctcata aatatcttga gtgacttcga cgtgattttg tctcaaatca
     5641 atgatttctt gactagtaca ttttaagtat ttctcaagga gtttcttctt gaatcgaaac
     5701 aacgtgcaac ctaaggtagg cacggagtgg cttacttcaa aggcctctag ggcaaggtct
     5761 tttcgatatt gcaatatgat tttttcgtct gttttaatgg gaatcaagtt aggaatttga
     5821 attcgatatt cagggacccc ttcaaaagcg gcttctgcac ggatccattg gcatgccttt
     5881 tcgtagattt tttcagaaag gtagtaggcg gcatcttttt gaagccccat cattttacgt
     5941 aaactgtggt gcctcattaa acacctgcta tggtctccat gggcatgtgt taagaaaaca
     6001 tggtctatgg gaatggctgt taaaggacat tctcccatat ctacggcaaa attaagctct
     6061 ggaagtaaat ggtaactagc gagacccgat attgaaaaac cagataaatt gaaccttttt
     6121 gtttgtaaat gagtctgttt taaggcatta tggatgtatt tattttcagt tgtcaaaagt
     6181 cgtatccgga tatggttctc ttttttacaa agatagataa aaaaacatct tttttttttg
     6241 atacgattta caaaaactat aggtatattc taacttggaa tacatagttt aaaaagacac
     6301 aaaaaacacc caaattgtcg tttttttaat tttgcataaa aggatctcgc atgaatagat
     6361 atgatttggt tttattaggc tttattttgg aacgcgaatg cagtggttat gatatcatga
     6421 ccgaaataag gaaacgggaa ttagaccgtt gggctaaaat cagtacatct actatttata
     6481 accgtttgat tcgtctagaa aagaatgagt acatcacagg tcgttctgaa cgtgatggta
     6541 atagacccga acgaatgatg tatacaatca ccgataaagg ccgtgaacta ttaaagaaag
     6601 aaattctaaa gcatttgact ggttttaatg atgatccaag aacgctgggt tatgctttct
     6661 tatacggtgt tgaaccgaag gaagcggttc gctccttaga agttcacgaa cgaagattat
     6721 tacaagaaat tgaacgctta aatgaaatga ttgcaaatga acctcgtccg actctttttt
     6781 cagaaggacc gttcttaaac tgtatgagcc gtgatcatat tttagtcgaa ttaaagtatg
     6841 ttcgcgcggc catttctatt ttgcgtgacc ctagcagaaa caaacagcta gatggctatt
     6901 tttacattaa ttttggtgca cgtcattttg ataagcgtga accatctacc gattcaaatc
     6961 gaga
//
LOCUS       DAOOBB010000094         6888 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_13063_length_6888_cov_4.025757, whole genome shotgun sequence.
ACCESSION   DAOOBB010000094 DAOOBB010000000
VERSION     DAOOBB010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 6888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6888
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13063_length_6888_cov_4.025757"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PLT31_05505"
     CDS             complement(<1..294)
                     /locus_tag="PLT31_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94630.1"
                     /translation="MNFKIILCLAITLIFIACDENSPTNSKPEQENSSSSEIPMMSSS
                     SEVIFSSSSVSCVESVDDVQANIYTTNIENFNISRGVENEGLRIFDKDQSILMT"
     gene            complement(353..2239)
                     /locus_tag="PLT31_05510"
     CDS             complement(353..2239)
                     /locus_tag="PLT31_05510"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPW94631.1"
                     /translation="MRVCFLTALFSFVVSSLLYSYAQSDYSITLSNLNTANKSLEMVY
                     VQGGSYQRGCSDGDTECETQEKPTHRVTLSDYYISKYELTNAQWQAVMADNTTKDNKP
                     KTSVTWYDAISFTCKLSEQTGKKYRLATDAEFEFAARGGNKSKGTLYSGSNNADDVAW
                     YSLNIKETCFGDFCFKSAQDVGTKAPNELGIYDMSGNVYEWMYDSYGTFSEGDLTNPT
                     GPATLHTQKVRRGGSFDQPASESRVSARKIRSIEGKDGSIGIRLAISVTDSRPEGMLD
                     PCDIQKPPPTGGKPGFYDERLINADGEAWVNELMENYINVLIIKENAAIYASVFNNTY
                     VTEDAKGQWYTLNSFSLNIVPSSGTQKKYIYYLVDSDNLSLMPEGGMPGRYQRRNIAD
                     VVGASKVTLPSITNPKKPEELAPAGYSIDMKNPPTDGRDSRLIEGPNNAWLQDNVALG
                     AGGTHRYRFDFDNEQYTRFFVYDPPSFTSLAKGSWYTVDNTFLRIFDDNGYVYDYLYT
                     VSEDGNTYYHISFQSYEVGDFRLFEKVSASAVPSWVEPSSSANTFYQGASTYIPPSED
                     NIISIKRDKRLYKDVSNVLDYNSNLLYYNLKGKPLGKIKPRESGVYIVKSQKTGKVWK
                     LLVK"
     gene            2651..5110
                     /gene="pheT"
                     /locus_tag="PLT31_05515"
     CDS             2651..5110
                     /gene="pheT"
                     /locus_tag="PLT31_05515"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPW94632.1"
                     /translation="MLVSINWLKRHIDLPESIEEIEKTLTAVGLEVEGKTEPGKNYEK
                     LVVAKVLTCEPHPDSDHLHVTTVNNGQEVIQVVCGAPNIKAGQTVVLAPIGAELPLPN
                     GEKLKMKKSKIRGVESFGMICAEDEIGLSDNHDGIMVLDNSIEAGTPFINLSFYDTTL
                     ELNVTPNRPDALCHRGVARELAAKFGRPLKKLEYSIIEESTPISSQISLSVDPDCGCT
                     CYVGRVLTDIEVKPSPAWLSTLLHSIEIPSINNVVDITNFILMDIGQPLHSFDMDQLS
                     GNSIHVRRAKADEKITTIDHKEYLLAPTDLAICDGNTPACVAGVMGGAESEINEKTKN
                     VFLESAYFNPTIVRKQSKRLGLSSDSSYRFERGIDPFMQEEASLYACSLIQQITNCKI
                     LKDAVSFTAPEHPKELKQVSIRPSRVQKVLGISPSADEIRKHLTSIGLVEEVPCCQAP
                     DKNADVLNFSIPGFRPDLEREVDLIEEVARLIGFDKIPYDLPLIRMQTNDLPVQEQIN
                     RKIRYSLSAMGLHECLSLRFTSKKLTQNVFGEPNEKDPRSTPAALLNPLSEDLGVIPT
                     SLLPNMLKYIAENEKNRPGTIRLFEVAKGQFSRKRESERDTGFDEIPLLAFAIAGHWK
                     TNALEEKAAPISFLDFKGVIISLFKRLGLSIDFKTPSVQESFLHPAQQAELVCGSYKL
                     GTAGTLHPAVMEKMDISYETCVLEVRLDFVEKAMQNRVTFVPFSRQVPSSRDISIEVD
                     EQMTHQSILERISGFNPKNLASIQLKSIYQGDKIAAGKKNMVYSLVYQAMDKTLTDEE
                     VNKVHNKLREKLVQNGDILLR"
     gene            5195..6367
                     /gene="dnaX"
                     /locus_tag="PLT31_05520"
     CDS             5195..6367
                     /gene="dnaX"
                     /locus_tag="PLT31_05520"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HPW94633.1"
                     /translation="MAYIAMARKWRPNSFQDMVGQEHIAKTLQNAIEGGRLHHAFLFT
                     GTRGVGKTTSARILAKTLNCMGSNPLTPCGVCNSCKDINGGNPMDVLEIDAASNTGVD
                     NIRELLERVQYAPMIGKYKVFIIDEVHMLSNGAFNALLKTLEEPPSHVIFIFATTEVN
                     KVPQTILSRVQRFDFKRLTTHQIIDRLQYICDQEKITTDVEALGIFAEKADGSMRDAL
                     TYFDQAYAFTGNDLTAKSVRSILGVPPNELYFMLIHAFAIHDIKECFEMVDKACSMGV
                     EFSPLLDGFGKFLRNLLYSRLDGVTAEELNITSELFEQLKTTASELSNGDLLRLSKIL
                     TDLQSKLHHTSNPRLLVETTFARMAWLDHMTDLRRALAAINDSPDASNEVLKKKSK"
     gene            6582..>6888
                     /locus_tag="PLT31_05525"
     CDS             6582..>6888
                     /locus_tag="PLT31_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HPW94634.1"
                     /translation="MQFKSEDKNESNWSYKQFLNNTEFQERLVRILEDYLQTTVELKV
                     AQRLLSESELQIEAKKKLTPYEKDLEEEKIFTILNNIFDTELVFSRKIPKIYSKTECD
                     "
BASE COUNT         2218 a         1354 c         1169 g         2147 t
ORIGIN      
        1 agtcattaga atagattgat ccttatcaaa aatacgtaac ccctcatttt ctacaccacg
       61 agagatatta aagttttcaa tattggtagt atagatattc gcttgcacat cgtccacact
      121 ttcaacgcat gaaacagatg aactcgaaaa aataacctca ctagagctac tcatcatagg
      181 aatctctgag ctactgctat tttcttgttc aggcttgcta ttagtaggcg aattttcatc
      241 acaggcgata aaaatcaaag taatagctaa gcacaatata attttgaaat tcatttttta
      301 tccaaagtaa agaagtttta aatatcacta aatataaatt aaaacgtttc ttttacttca
      361 ctaatagctt ccaaactttt cctgtttttt ggctttttac aatatagacc cctgattctc
      421 ttggttttat ttttccaagt ggctttccct tcaaattata ataaaggaga tttgaattat
      481 aatcaagaac attggataca tctttatata atcttttatc tctttttata gaaatgatat
      541 tgtcttctga aggaggaata taagtggaag ctccttgata gaaagtattt gcagaagatg
      601 atggttctac ccaactagga actgcacttg ctgatacctt ctcaaaaagt ctaaaatccc
      661 ctacttcata actttgaaat gagatatgat aataagtatt accgtcttca gataccgtat
      721 ataaatagtc ataaacataa ccattatcat caaaaattct taaaaaagta ttatctacgg
      781 tgtaccaaga acctttagca agagaggtaa aagacggtgg atcataaaca aaaaatcgcg
      841 tatattgctc gttatcaaaa tcaaacctat accgatgtgt gccgcctgca ccaagagcca
      901 cattatcttg aagccaagca ttatttggcc cttctattaa acgagaatct cgtccatccg
      961 tcggtggatt tttcatatca atggaataac cagcaggagc taattcttct ggttttttgg
     1021 gattagtaat agagggaaga gtgactttag aagctcctac cacatctgcg atatttcgtc
     1081 tttgatatct tccaggcatt cccccttctg gcatcagcga cagattatca ctatctacca
     1141 aataatagat atattttttc tgagttcccg aactaggaac aatattaaga gaaaagctgt
     1201 ttagagtata ccactgccct tttgcatctt ctgtaacata cgtgttatta aaaacagagg
     1261 catagattgc cgcattctct ttaataatca aaacattaat gtaattctcc attaattcgt
     1321 tcacccaagc ttccccatca gcattaatta aacgctcatc ataaaaccct ggtttcccac
     1381 ctgtaggagg cggtttttga atatcacaag gatctaacat tccttctgga cgagaatcgg
     1441 ttacagaaat agctaagcga atgccaatgg aaccatcttt accttctata gaacgaatct
     1501 ttctagctga aactcttgat tctgaagcag gttgatcaaa gcttcctcct cgacgcacct
     1561 tttgtgtatg taaagtcgct ggccctgtag gatttgttaa atctccttca ctaaaggtac
     1621 catagctatc atacatccat tcatagacat ttccactcat atcgtaaata ccaagctcat
     1681 taggtgcttt tgttcccaca tcttgagcac ttttaaaaca aaaatctcca aaacaagtct
     1741 cttttatatt gagagaatac caagcgacgt catcggcatt attgctccca gaataaagtg
     1801 ttcctttact tttattccct ccacgtgcag caaattcaaa ttctgcatct gtggctaatc
     1861 gatatttttt tccagtttgt tcgctcaact tacaagtaaa agaaatggca tcataccatg
     1921 ttacacttgt ttttggttta ttatccttcg ttgtattatc agccatgaca gcttgccatt
     1981 gagcattcgt aagttcatat ttggagatat aataatcact taacgtcaca cgatgagttg
     2041 gcttttcttg cgtctcacat tctgtatcgc catcagaaca gcctctttga taggatccgc
     2101 cctgaacata aaccatctct aatgacttat ttgctgtatt taagttgctc aaagttatcg
     2161 aataatcgct ctgagcataa gaatacaaaa gcgaactcac cacaaaagaa aaaagggctg
     2221 ttaaaaaaca aactcgcata gaagcctcca aagaaggagt ttttgataat tagaatttat
     2281 ataaataaaa gaaattcaaa gggaatttgc taatcattca aaaaatagca ttgtacgttg
     2341 ttgtaatttt ttaatacaaa ataaggaaaa agagcatcag tttttcaaag ataatcgcgg
     2401 acagtaaatc atattttttt caaaaaaaag taacatggat cctatttttc cataaaaata
     2461 ttacacatct ttgtaagaaa ccctctttta acaaacctga attgactttt ctaaataatt
     2521 aaaactgata atcgtgtaat ttaacgcctt aaacatcgat tcaagcttag agatctcact
     2581 ccatttcata taaaatgatt ttttttgtta aattttgcac tgaatttttc agagatgtta
     2641 aggaattcat atgcttgtat ctattaattg gctcaaaaga cacattgacc tccctgagtc
     2701 tattgaggaa atagaaaaga ctcttactgc tgttggtctt gaagtagaag gaaaaaccga
     2761 gccaggtaaa aactatgaaa agctcgttgt cgccaaagta ttaacttgtg aacctcaccc
     2821 tgatagcgac caccttcacg tgaccacagt gaacaatggg caagaagtca ttcaagtcgt
     2881 ttgtggcgca cctaatatta aagcaggcca aacagttgtt ttagcaccca tcggagcaga
     2941 gttacctcta cccaatggtg aaaagctcaa aatgaaaaag tcaaaaattc gtggtgtaga
     3001 aagttttgga atgatttgcg cagaagatga aataggtctt tccgataatc atgatggaat
     3061 tatggttctt gataattcta ttgaagcagg aacgccattc atcaatcttt cattttatga
     3121 caccacactg gaactaaacg ttacacctaa ccgtccagat gcgctttgcc acagaggcgt
     3181 tgctcgtgaa ctagcagcta aatttggtcg tcctctgaaa aaattagagt attctattat
     3241 tgaagaatct actcctattt caagtcaaat ttccctttct gtggatcccg attgcggctg
     3301 tacttgttat gtaggccgag ttttaacaga tattgaagta aaaccatctc cagcatggct
     3361 atctacgtta ctacactcaa tagaaatccc ttcaattaat aacgtagtgg atattaccaa
     3421 ctttatatta atggatattg ggcaaccact tcatagtttt gatatggatc aattatctgg
     3481 taattctatt catgtacgtc gcgcaaaagc agatgaaaag attaccacca tcgatcataa
     3541 agaatatctt cttgctccta cagatttagc gatttgtgat ggaaatactc cagcttgcgt
     3601 tgctggtgtc atgggcggag cagaatcaga aatcaacgaa aaaacaaaaa atgtattttt
     3661 agaaagtgca tattttaatc cgaccattgt tcgcaaacaa agtaagcgtc tcgggctctc
     3721 ttctgactct agttatcgtt tcgaaagagg gatagatcca tttatgcaag aagaagctag
     3781 tttgtatgct tgttctttaa ttcagcaaat cacaaactgt aaaattctaa aagacgccgt
     3841 ttcattcacc gctccagaac acccaaaaga attgaaacaa gtttctattc gtccttctcg
     3901 cgtacaaaaa gttcttggga tctccccttc tgcagatgaa attagaaagc acttaacaag
     3961 cattggctta gttgaagaag tcccttgttg tcaagctcca gacaagaatg cagatgtttt
     4021 aaacttctct atccctggtt tcagacctga tctcgaaaga gaagtagatt taattgaaga
     4081 agtggctcgc cttattggtt ttgataaaat tccatacgat ttacctctca ttcgtatgca
     4141 gacaaatgat ttgccagttc aagaacagat taatcgtaaa attcgttact ctctttctgc
     4201 aatgggatta cacgaatgct tgtcccttcg tttcacaagt aaaaaactca ctcaaaatgt
     4261 ttttggtgaa ccaaatgaaa aagaccctcg tagtacacct gccgctttac taaacccact
     4321 ttctgaagat ctcggtgtaa ttccaacttc tcttttgccc aatatgttaa aatatattgc
     4381 agaaaatgag aagaatagac caggaaccat tcgtttattt gaagtcgcta aaggccaatt
     4441 ctcaagaaaa agagaatccg aaagagacac agggtttgat gaaatcccat tactcgcctt
     4501 tgctatcgca gggcactgga aaacgaatgc tttagaagaa aaggctgctc ctatttcatt
     4561 cctagacttc aaaggcgtta ttattagtct atttaagaga ttaggcttat ctattgattt
     4621 caaaacgcca agcgttcaag aatcattcct tcatccagca cagcaagctg aactggtctg
     4681 cggttcttac aaattaggta ccgcaggaac tcttcaccca gccgttatgg aaaagatgga
     4741 cattagttat gagacttgtg tcttagaagt tcgcttagat tttgttgaaa aagcgatgca
     4801 aaatagagtt acttttgtac cattcagccg tcaagtgcct tcttctcgag atatttccat
     4861 tgaagtcgat gagcagatga ctcaccaatc gattttagaa cgaatttcag gtttcaaccc
     4921 taaaaatctc gcctctatcc aactaaaaag tatttatcag ggcgataaaa tcgctgcagg
     4981 taaaaagaat atggtttatt ctcttgttta ccaagctatg gacaagactc tcactgatga
     5041 agaagtgaat aaagttcata ataaacttag ggaaaagctt gttcaaaacg gtgatattct
     5101 tttaaggtaa tttctaccaa gaatgatctt gtttttcttg gtgttttaac attcttcaaa
     5161 ctttattttt gataaagttt actattattt gagtatggca tatatagcaa tggcccgtaa
     5221 gtggcgtcct aattcgtttc aagatatggt gggtcaagaa catatcgcta aaacccttca
     5281 aaatgcaatt gaagggggac gcttgcatca tgctttttta tttactggga ctcgtggagt
     5341 cggcaaaacc acttctgctc gtattttagc caaaacatta aattgcatgg gttcaaatcc
     5401 attaacacct tgtggcgttt gcaacagctg taaagatatt aatggcggca acccaatgga
     5461 cgttcttgaa attgatgccg ctagtaatac aggtgttgat aatattcgag aactactaga
     5521 acgtgtccaa tatgcaccca tgatcggtaa atacaaggtt tttattattg atgaagtgca
     5581 tatgctttct aatggagctt ttaatgctct attaaaaaca ctggaagagc ctccttctca
     5641 tgtgatcttt atttttgcga ccactgaagt caataaagta cctcagacga ttttatctcg
     5701 tgtgcagcgt tttgatttta aacgcctcac cactcatcag attattgatc gtttgcagta
     5761 tatttgcgat caagaaaaaa ttaccacaga cgtagaagct cttgggatat ttgctgaaaa
     5821 agcagatggt tccatgcgag atgctctcac ctacttcgat caagcttatg cttttacagg
     5881 aaatgactta acagcaaaat ctgtgcgctc cattttagga gttcctccta atgagctcta
     5941 ctttatgttg attcacgcct ttgccattca tgatattaaa gaatgctttg aaatggtaga
     6001 taaagcgtgt tccatgggtg ttgaattttc tcctctttta gatggttttg gtaaattttt
     6061 aagaaattta ctctattctc gcttagacgg agtcactgct gaagagctaa atatcacttc
     6121 agaattattt gaacagctca aaacaacagc ttcggaatta tcaaacggag atttactacg
     6181 tctttctaaa attttaactg atctccaaag caaattacat cacacgagca accctagatt
     6241 actagtggaa acaacgtttg ctcgaatggc ttggctagat catatgaccg atttaaggcg
     6301 agcgttagct gcaatcaacg actccccaga cgcttctaat gaagtgttaa aaaaaaaaag
     6361 taagtgaagt taccgcatct cttgattcta tcacgagtac tgcaccaact tcttcttggg
     6421 aaattgctcc tactcctcag tacactcgct atgaaatttc atctgcttgg aatacgatct
     6481 gccgtcaatt aagcgatgat catgatttac tttttgcagc cgtcctgaat ggcacctccc
     6541 ttttaacacc cgatttaaaa gccactccat ttcccgtgac attgcaattt aaaagcgaag
     6601 acaaaaatga aagcaactgg tcttacaaac aatttttaaa taacacagag tttcaagaac
     6661 gccttgtccg tattttagaa gattacctcc agaccacagt tgaacttaaa gtagcccagc
     6721 gtcttttatc tgaaagtgaa ttacaaattg aagccaaaaa aaaattgact ccgtatgaaa
     6781 aagacttaga agaagaaaaa atatttacga ttttaaataa tattttcgac actgaattag
     6841 tattttcaag aaaaattcct aaaatttact ctaaaacaga atgcgata
//
LOCUS       DAOOBB010000095         6885 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_13070_length_6885_cov_5.271157, whole genome shotgun sequence.
ACCESSION   DAOOBB010000095 DAOOBB010000000
VERSION     DAOOBB010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 6885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6885
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13070_length_6885_cov_5.271157"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PLT31_05530"
     CDS             complement(<1..270)
                     /locus_tag="PLT31_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HPW94635.1"
                     /translation="MPALTLERLLSSLGFGSRKDSRALIRMGVVEIDGIIEEDPFIEL
                     EKRPDYITVNGEQIPTFEDLYIMMHKPLGYECSHNPRYHDSVFSLL"
     gene            complement(270..1514)
                     /locus_tag="PLT31_05535"
     CDS             complement(270..1514)
                     /locus_tag="PLT31_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94636.1"
                     /translation="MIGISILAGLLFLGALLLFFPLRIRFYFVGAKKDSLIEIRLFKW
                     KLFTTKENPSDETTESNVGSDATNKDALDERDETTEIAGIDGATAESELFENTDRNVS
                     EATDYDEDDESLKNSGVSTTESEAAALQTAESKSRENQNRNDETHEILNSDAVESSTY
                     PHSETHEAMSCRATETVENTESDVSDEDAKITASTESTKADEKCENPISESAEKFVDS
                     GEETLAKGASMDDSEMSETIESDVTDEATNTDASKGKDSKASKKLRELTEKEFLTLMF
                     NPKLHSMVLRMAWKLLRRAWRLFTIRFEHTVVQGIRMEYDEMGVVSAFTSFFTSQFSL
                     FKNWDIQMDWLKEKEPEIRGSILIRFRLFFFLLWSLMALFFAARVYFWYRRNKKRFIE
                     NPLPFRLVFWRRKIIDLIAAEE"
     gene            complement(1517..2098)
                     /locus_tag="PLT31_05540"
     CDS             complement(1517..2098)
                     /locus_tag="PLT31_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPW94637.1"
                     /translation="MISIKKVITIFLVSAAFLFSAENPVSVIKKKDGELQSLIKKSSL
                     TKNQKERIKTLLNDSFDFQLLAKKSLANSVWNAQTPAALDTFVNEFQRMVRNSSAKKL
                     EMYRADSTVYEEAKLKGTDEARVIAHVWYKGKESVLEYKMSLVNDSWKAWDLVIDDLS
                     TARNYKEQFSTILKTKSFNDLIQIIREKANEAE"
     gene            complement(2108..3472)
                     /locus_tag="PLT31_05545"
     CDS             complement(2108..3472)
                     /locus_tag="PLT31_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPW94638.1"
                     /translation="MVIRVLAVLSLVLSFAWGESYDCLRFVNESIKKDPMVAEKRYSS
                     MKKKEQLATLKSEVILPTFTFSMMVGPAPGLKEHVDSWGDTVEAWDFRKMGPYWGTQI
                     KATQPLNLGQYNVGKKAIQADIRQHELGLLHGEHKKEVELQSYYYNYLLALEMNRLAK
                     DVQKQINNAYEKLEEALDNDEPNVSQMDLLKLKSNMYVVQEAVSDADRGLKQVMLAIR
                     FSLQLEEGALFSTVDSVLTIRPEPFLSLEEYKNLALKFHPELKQLKAGLEARSYQMDL
                     AEAKLAPEFFIMAEFEYVKSWAGNRQVIQKNAFAQDAVNTLSGTFGIGLRYRLNFWKG
                     WQNYRKARIEHNALRMKENYAAQGLLLKIEEQYDKVVSIKEKVESMKTSLRATEAILK
                     GTAIQYDLDPSNAEKLLSAYTNNVNLLKKYYFAICSYNVEFAELIFRTGLSLEEFHST
                     YVSK"
     gene            complement(3472..4170)
                     /locus_tag="PLT31_05550"
     CDS             complement(3472..4170)
                     /locus_tag="PLT31_05550"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HPW94639.1"
                     /translation="MKISLRLSSGVLFFFYASALILWLVFIAIWVVYQKKWDTLLTQE
                     QTLREEILELEEYGKWTLDYVKIEKGLTFLSKNKINEQSRVELTEQIWKISRTYNIDP
                     LLILSIVAQESHGNPKARGRMRSGAESGAFGLMQIKLETAKSIGRRFGYKIESPEDLM
                     APDVNVVIGTAYLMRLIGKYQNLKHAIIAYNIGPGLLDSHLKKNHPLPTRYYERVISK
                     YWNLVSNSNFLDLR"
     gene            complement(4160..4849)
                     /locus_tag="PLT31_05555"
     CDS             complement(4160..4849)
                     /locus_tag="PLT31_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94640.1"
                     /translation="MKSDWMDILVDDAGDEVVITLRGTFGLLQLPGVREKLEMLIKGP
                     GCFFFLNIESAHFIDDAYLELFLNLLNRIKKQNAMLVFLSSKEEHRNYFSRFSHVLEL
                     YDNRKSFRRSGLLKQLKQVGINYSKQTGLRLSPGVAIALFVLLLGWFVTLFSIVYIQN
                     KEILEQQAQIISLENQKKRSIQEIERLKSSLGSLQHLGLVVDSTTSHAFGSIRDWVGY
                     LELLENSRREN"
     gene            complement(4846..6591)
                     /locus_tag="PLT31_05560"
     CDS             complement(4846..6591)
                     /locus_tag="PLT31_05560"
                     /inference="COORDINATES: protein motif:HMM:NF020660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1926 domain-containing protein"
                     /protein_id="HPW94641.1"
                     /translation="MYLPGSILEAMVKYCPQEIAWIKARLQEGRLELVGGGYYDSMSP
                     FFPSRLQSLQLKTHRQYITKIFQVEPSGYFNSSMVWEIGMTELLAHFDFKYTLVNDDS
                     LQFALGRTTPVSGWFTTEDKGTMIRLISVSTGLSKAFTEEEPSIFEEEIQKLPKNDKC
                     VVVSLEVPLDNALTITSFFTSLEQKLSLLDHQTWTVAHVIEQQMTEGKVNLVSALGSN
                     LGLPAGAHSCRELLIRRPEINFLHKSFLAVHRYALEKLSGNDLEKIEEMLLPLMAPFF
                     YRDLYGGKGMKNPGVRWKAHKDLIGVFLAVEELESFDGLRIEVSDYLLDGQKQVWVNN
                     SEISMLIDHMRGAYLRSLNYKPSGVNLFNAMRDDGDISLGFLDHFVPNKINSVSGFQS
                     AVEDRSGALTYPCDYELKREPEAVHLLFHSEQVFSSPETKHIFHVDKHFSLLSKHAAF
                     DLDYRLTNSTYLDFKGYFGTELELGMRGFDSKTQLMKINGTKISLMQDVPILYPNAET
                     FELQDRLLAWFIRFNFSQATRILVSPIMGMNQFASPLAVQGLRIFFFWDVELSALESS
                     SLGMKIKLSKRRVLL"
BASE COUNT         2305 a         1401 c         1024 g         2155 t
ORIGIN      
        1 caataaagaa aagaccgagt catgatagcg aggattatga ctacattcat atcctaatgg
       61 cttatgcatc ataatgtaca agtcttcaaa agtagggatt tgttccccat ttactgtaat
      121 ataatcagga cgcttttcta actcaataaa aggatcttct tctattatcc catcaatctc
      181 aacaactccc atacgaatta aagcacgaga atcttttctt gaaccaaagc ccaaagaaga
      241 aagcaatcgt tctaaagtca aagcaggcat tattcctccg ccgcaataag atctataatt
      301 ttacgtcgcc aaaaaactaa acgaaatgga agtggattct ctataaaacg ctttttattt
      361 cgacgatacc aaaaatacac tcgagccgca aagaataaag ccattaacga ccaaagtaag
      421 aaaaaaaaca atcggaaacg aattaaaata gaacctctaa tttctggttc tttttctttt
      481 aaccaatcca tttgtatatc ccaattctta aacaaagaaa attgagatgt aaagaaagaa
      541 gtaaaagcag acaccacacc catttcatca tattccatcc gaatcccttg aacaaccgta
      601 tgttcgaaac gaatggtaaa taaacgccac gcacgacgca gtaatttcca cgccatacga
      661 agaaccatcg aatgaagttt tggattaaac atcaatgtca aaaattcttt ttcagtaagt
      721 tctcttagtt tctttgacgc cttactatcc ttaccctttg aagcatcagt attggtcgct
      781 tcgtcggtta cgtcactttc aatagtttcg gacatttccg aatcatccat tgaagcacct
      841 ttcgccaaag tttcctcgcc tgagtctaca aacttttcag ccgattcgct tattggattt
      901 tcacactttt catcggcttt cgtgctttcc gttgatgcag taatttttgc atcttcgtcg
      961 gaaacatcac tttctgtatt ttctactgtt tctgtagcac ggcaactcat ggcttcatgc
     1021 gtttctgaat gagggtacgt ggacgattca acggcatcac tatttaaaat ttcgtgagtt
     1081 tcatcatttc tattttgatt ttcgcgagat ttcgattcag ccgtttgcaa tgcagcagct
     1141 tccgactccg tcgtcgatac accactattt ttcaaagatt cgtcatcttc atcgtaatct
     1201 gtagcttcag aaacatttct atctgtattt tcgaataatt cagactcagc tgttgcacca
     1261 tctatgccag cgatttcagt agtttcgtca cgttcgtcga gagcatcttt attagtagcg
     1321 tcactcccta cattactttc agtagtttcg tcagatggat tttcttttgt agtaaagagt
     1381 ttccacttaa aaaggcgtat ttctattaga ctatcttttt ttgcaccgac aaaatagaat
     1441 cggatacgca aaggaaaaaa aagaagcaaa gccccaagaa aaagaagccc tgctaaaata
     1501 gaaataccta tcaatactac tccgcttcgt ttgccttttc acgaatgatt tgaatcaaat
     1561 cattgaagct ttttgttttt aaaatggtag agaattgttc tttgtagttt cttgctgtag
     1621 ataagtcatc aatcactaaa tcccaagctt tccaagaatc gttcaccaaa gacattttat
     1681 attccaaaac agattctttg cccttatacc aaacatgagc aatcactcta gcttcatcag
     1741 tccctttgag tttagcttct tcgtatacgg ttgaatccgc gcgatacatt tctaattttt
     1801 ttgcacttga attacgcacc atgcgttgga attcattcac aaatgtatca agagcagctg
     1861 gagtctgagc attccatacg gaattagcta aagatttttt ggctaaaagc tgaaaatcaa
     1921 aagaatcgtt taataatgtt ttaatcctct ctttttgatt ttttgttaaa gaggacttct
     1981 ttattaaaga ttgtaattca ccatcttttt tcttaattac actgactgga ttttctgccg
     2041 aaaacaaaaa ggctgctgaa accaaaaaga ttgttattac tttttttatt gaaatcatat
     2101 aatatcctta ttttgaaaca taggtactat gaaattcttc taatgataaa cccgttctaa
     2161 aaataagttc agcaaattca acattataag aacaaatagc aaaataatat ttttttaata
     2221 aattaacatt attcgtataa gcagacaata gcttttccgc atttgaagga tctaaatcat
     2281 attgtatcgc tgttcctttt aagattgctt ctgttgcccg tagacttgtc ttcatgcttt
     2341 ctaccttttc ttttatagaa accactttat catattgttc ttctattttt aacaataagc
     2401 cttgagccgc ataattttct ttcattcgta aagcattatg ttctattcta gcttttcgat
     2461 aattctgcca acctttccag aaattcaaac gataacgtaa gcctatacca aaagtcccag
     2521 acaaagtatt cacggcatct tgagcaaaag catttttctg aattacttgg cgatttcctg
     2581 cccaagattt tacatattca aattcagcca taataaaaaa ttcaggagcg agttttgctt
     2641 ctgctaaatc catctgataa gatctagctt ctaaacctgc tttaagttgc tttaattctg
     2701 gatgaaactt taaggctaag ttcttgtatt cttctaaaga aagaaaaggt tctggacgaa
     2761 tcgttagcac gctatctacc gtagagaaaa gagccccttc ttctaattgc aaagagaatc
     2821 gaatcgctaa catcacttgc tttaaaccac gatccgcatc agagacagct tcttgaacca
     2881 catacatatt cgactttaat tttaagagat ccatttgaga aacattaggc tcatcgttat
     2941 ctaatgcttc ttctagtttt tcataggcat tatttatttg tttttgaaca tcttttgcca
     3001 aacgattcat ttctagtgct aatagataat tgtaataata ggactgtagc tctacttctt
     3061 ttttgtgttc tccatgaagt aggcctaatt cgtgctgccg aatatcagct tgaattgctt
     3121 tttttccaac attatattgc cctaaattaa gaggctgagt ggccttaatt tgcgtacccc
     3181 aataaggacc catctttcta aaatcccatg cttcaacagt atcgccccat gagtctacat
     3241 gctcttttaa tccaggagca ggacctacca tcatagaaaa agtaaacgta ggtaaaataa
     3301 cttctgattt taatgtagcg agctgttctt tcttcttcat tgaagaataa cgtttttctg
     3361 ccaccatagg atcttttttg atggactcgt tcacaaagcg aagacaatca taagattctc
     3421 cccatgcgaa agagagcacc aaagataaaa ctgctaagac acgaataacc attagcgtaa
     3481 atctagaaaa tttgaattag ataccaaatt ccaatattta gaaataactc tctcataata
     3541 gcgagttggt agaggatgat tcttttttaa gtgagaatct aataaaccag gtccaatatt
     3601 atatgcaata atggcatgct ttaaattctg atacttccca attaaacgca ttaggtaagc
     3661 agtcccaata acgacattca catctggagc cattaaatct tctggagatt caattttgta
     3721 accaaaacga cgtccaatac tctttgccgt ttctaattta atctgcatca aaccaaacgc
     3781 tccagattca gctcccgatc gcattcttcc tcttgctttt ggatttccat gactttcttg
     3841 agcgacaata cttaaaatca aaagtgggtc aatattatac gttctagaga ttttccaaat
     3901 ttgttctgta agctcaactc gactttgttc attgatttta ttctttgata aaaaggtcaa
     3961 tcccttttca attttcacgt aatctaaagt ccactttcca tattcttcta gttctaaaat
     4021 ctcttctcga agtgtttgtt cttgagtcag aagagtatcc catttctttt gatagacaac
     4081 ccaaatagca ataaaaacaa gccagagaat caaagccgaa gcataaaaaa agaagagcac
     4141 tccagaggat agtcgaagac taattttcac gcctggagtt ctccaaaagc tcaagataac
     4201 cgacccagtc tctaatagaa ccaaaggcat gagatgtcgt tgaatccacg accagaccaa
     4261 gatgttgtaa tgaacctaaa gatgatttca atcgttcaat ttcttgaata gatctctttt
     4321 tctgattttc taaactaata atctgcgctt gttgttcaag aatctcttta ttttgtatgt
     4381 aaacaatcga gaataaagta acaaaccaac ctagtaaaag aacaaacaaa gcaatcgcca
     4441 caccagggct caaacgaagc cctgtttgct tactgtaatt tatgcccacc tgttttaatt
     4501 gttttaaaag accagaacgt ctaaacgatt ttcgattatc atacaattct aaaacatgag
     4561 agaatcgaga aaaataattt cgatgttctt ctttcgaaga caaaaaaaca agcattgcgt
     4621 tttgcttttt aattctattc aaaagattta agaacaactc caaataagca tcatcaataa
     4681 agtgagcact ttcaatattc aaaaagaaaa aacaaccagg acctttaata agcatctcta
     4741 gtttttcacg tacccctggc aactgcagta atccaaaagt cccacgtaaa gtaataacaa
     4801 cttcatctcc ggcgtcgtct actaatatgt ccatccaatc tgacttcata gtaaaactct
     4861 ccgtttagat aatttaattt tcattcctaa cgaactagat tctaaagcgg ataattcgac
     4921 atcccagaaa aagaatattc ttaacccttg caccgcaaga ggagatgcaa attgattcat
     4981 tcccataata ggcgaaacta gaattcgagt agcctgagaa aagttaaatc gaataaacca
     5041 agctaacaat cgatcttgta actcaaacgt ttctgcattt ggatataaaa tgggaacatc
     5101 ttgcatcaaa gaaatttttg tcccattgat tttcatcaat tgcgtttttg aatcaaaacc
     5161 tctcattcca agctctaatt ctgtcccaaa atagccctta aaatcaagat atgtagaatt
     5221 agttaaacga taatctaaat caaatgccgc atgctttgat aaaagcgaaa aatgcttatc
     5281 cacatgaaaa atatgtttcg tctctggaga agaaaaaact tgttccgaat gaaataacaa
     5341 atgaactgct tcaggctccc gtttcaattc ataatcacat ggataagtta atgccccaga
     5401 acgatcttct actgccgact gaaacccaga aacggaattg attttattag gcacaaaatg
     5461 atccaaaaaa ccaagagaaa tatctccgtc atcacgcatc gcattaaata aattaactcc
     5521 cgaaggctta taattcaggg atcgtaaata agctccacgc atatgatcta tcaacatcga
     5581 tatttcagaa ttattcaccc aaacttgttt ttgcccgtct agcaaataat ccgatacttc
     5641 tattcgaaga ccatcaaaac tttctaattc ttctaccgct aaaaaaacac ctattaaatc
     5701 tttatgagct ttccaacgga ctccaggatt tttcattccc ttaccaccat acaaatcccg
     5761 atagaaaaaa ggtgccatta gaggcaaaag catctcttct attttttcta aatcattacc
     5821 acttaacttt tctaaagcat aacgatgaac cgcaagaaat gatttatgca aaaaattaat
     5881 ctcaggacga cgaattaata attctctaca actatgagct ccagcaggta atcccaaatt
     5941 agaacctaat gcactcacta aattcacctt cccttcagtc atctgctgct caatcacatg
     6001 agcgacagtc catgtttgat gatctagtaa agaaagtttc tgttctaaag atgtaaaaaa
     6061 tgaggtgatt gttaatgcat tatctaaagg aacttctaaa gaaacaacaa cacacttatc
     6121 attttttgga agtttttgta tttcttcttc aaaaatagaa ggctcttctt ctgtaaaggc
     6181 cttagataac cccgtcgaaa cagaaatcag gcgaatcatt gttcccttat cttctgtagt
     6241 aaaccaacca gaaacaggcg tagtacggcc taaagcaaac tgcaaactat catcgttcac
     6301 taaagtgtat ttaaaatcaa aatgagctaa tagctccgtc atcccaattt cccaaaccat
     6361 cgatgaatta aaatagccgc ttggttctac ttgaaaaatt tttgtgatat actgacgatg
     6421 cgtttttagt tgcaaagact ggagacgaga agggaaaaaa ggagacatgg aatcataata
     6481 accgccacca actaattcta aacgtccttc ttgaaggcgt gctttgatcc aagcaatttc
     6541 ttgagggcag tattttacca ttgcttcaag aatagagcct ggtaaataca aagataagcg
     6601 tataggagtt ctatctaaaa aacgctttaa ctctattgca aaagctttta tttcctctac
     6661 tggaataaca tttaatggag agaagacatc ttttacaaaa gaagaattga aagtttaatt
     6721 ttatacataa atccttatat ctctttaaaa atcaatataa taagaaatca atgagaatag
     6781 atatgattaa gaaatataaa aaccgaaacg taataaaaat caagcgatta ataacaggaa
     6841 tgattcagca aaagtaattc gtaaataaga gcagattaac agaga
//
LOCUS       DAOOBB010000096         6848 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_13163_length_6848_cov_4.914912, whole genome shotgun sequence.
ACCESSION   DAOOBB010000096 DAOOBB010000000
VERSION     DAOOBB010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 6848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6848
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13163_length_6848_cov_4.914912"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PLT31_05565"
     CDS             complement(<1..181)
                     /locus_tag="PLT31_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPW94642.1"
                     /translation="MKVLIIGSVYPRFDEDSEVPWLRTSVKKLKEAGVQVQILCPSYQ
                     GLKSHEIDGIPVHRFR"
     gene            complement(178..645)
                     /locus_tag="PLT31_05570"
     CDS             complement(178..645)
                     /locus_tag="PLT31_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94643.1"
                     /translation="MMKKIIFNGIKNHKYDLILYPAEFFELRIQEEIVRTDEYKSSFV
                     YVEMDFNRIKATLKDDTEELAFWEAALRSMAIKKRGSDVLGFLQDDAGIGLLLLDSKM
                     EGWIRLQGRISEFCRLTIGDISNTLQKGTRAFVYPACLKDFVIPKPNGEEKST"
     gene            675..2549
                     /locus_tag="PLT31_05575"
     CDS             675..2549
                     /locus_tag="PLT31_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HPW94644.1"
                     /translation="MQRLLKLKKALKSSFITTSVEVLRSTKVPIRYRTLTPEEIETLE
                     KNGNRCDDWSLVSVEPNFSPERINQSIFVGEVRLPAFFGTVLLPGDVSFPTGIYYSLV
                     HNCTIENALVHRVAMLSNVLVRFGAVLQNVGSIVSSGKISYQTGSDIVIGNEMGGRPL
                     YVFPDITDELIDAQLLQKMNEEVQGSFAELINTWRQEVSLPFGVVDKGAVISNTNIVR
                     NSWIGPHARIEGASKIRNTVVLSSLEEPCNIYDSVIIENSNVQEGVKVHSGALVKNSV
                     LMKRVKVGNKAIINSSIISSCCHIEEAEVTCSYVGPLTQMHHHSLLISTLWPMGGGNL
                     GYGANVGSNHTGRRPDQELYAGQGLFFGLGVNIKFPANLKDSPFSIIATGVTCEPQRL
                     MFPFSLIKKGTHSDPSKSFLNELIPGWNYAKNAYALDRNFYKYSIRGKGFVDVSFGAL
                     SQSELVKNVFDAYQRLQVKTIKDVYLKEDILGLGSNYLRETVRQNALKTYREFLERFV
                     LDACVSAMEADSSLLMESPKDFRKSFSGEVLREITKDWAFPETVADLLKKYKALEKEW
                     FDCVFKGLDRDAVRGREIFDDYDEVHLPDKAFIDFEKQRIEESNKRVALLSKELRLEA
                     "
     gene            2558..3118
                     /locus_tag="PLT31_05580"
     CDS             2558..3118
                     /locus_tag="PLT31_05580"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HPW94645.1"
                     /translation="MFFWLVVLLGIEFVFRVALEIREARLFSRKNRFALFRVIPFIND
                     FVPFPENRDQTEISNPFVKFHEEVHEKLHHSMTRLLLKGVFFFLSILGIFYVIGQLRM
                     SLVEVVILFHLALFFLQIPYHYYIWQQEFEADAYAAKKTSITQAKKQFRLLANQEIPY
                     STFFALLYREHPPAALRLKKVISKFS"
     gene            3354..3932
                     /locus_tag="PLT31_05585"
     CDS             3354..3932
                     /locus_tag="PLT31_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94646.1"
                     /translation="MRKYIVILFYILCGCQGNTTSDDELAKNNPMAFSVLLAMFEQES
                     APDFDSNVLAYAKIPAFDDDSAGINPLPFNDIVTYFPKTASLILKHKKCDFYIIKQKK
                     DNSNSFDVLHSISPEGALIYHFVPTTPKGDVCKAIRSVDYWAFLVEDCSRTLNQSIPI
                     RYKTHESSSWKCDDVIPFNTQEEWISPSILKI"
     gene            4330..6213
                     /locus_tag="PLT31_05590"
     CDS             4330..6213
                     /locus_tag="PLT31_05590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HPW94647.1"
                     /translation="MKYLTYTLWIIFLASSLVFSRPAKPGVISVKQSDSSSLSIREFG
                     DEHYHYTTTEDDYLIVKNEKGIYVYVDENGSPSKIEAKNKEKRTANENTFLKGIDQKK
                     VKAKHKSKRPSSYPQQDRKLNFNRQSRSIDVSTTVPQLLKRPTAKLWTAGERYIPVLL
                     VSTLDKAAGDSALFHRFLNEPGFSEQGNIGSLRDYFLISSDSLFKPHFDVFPIQLNKK
                     LTDYVNGGDFLEGQFIKEAIDLMVQKPYFSGSKYCLSETTVDGFLFLFPGKEEDALKI
                     HEDFWGHQYWMEWNGSTNNYRAYQSGGYSFNAYAFLAQKEDGKPSNINQMGIFAHEFS
                     HVMGLSDLYGSDQNGNLVPGPTPWDVMTQGMYNSSGRKPPKYSGFERESMGWVTLTEF
                     SANEEIYTLLSLDQRQAYSVTNPQNNNEYFVIEYRPKVGFDSGINNTGVLVWYIDYDS
                     NVWDNNNPNDDPSHQRVAVKEVLLGTQSSKTSYSPFTFYDDSSSTVPGIFSFIKGTGD
                     KVCFTTSTNKMGFVCEESSSSVIASSSSVEESSSSATVLIENHLRKSNFKIAVLGRNL
                     QVYLPIVGEKVLKVMDVQGNLVHLGRFTGDNETLTLQNKGVYYLQISLNNELLVSRPV
                     KIR"
     gene            6547..>6848
                     /locus_tag="PLT31_05595"
     CDS             6547..>6848
                     /locus_tag="PLT31_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-beta-glucanase"
                     /protein_id="HPW94648.1"
                     /translation="MNLKSIIGIVAILSVSLFAQEPYSWGNVRMDGGGFVSAVIAHPK
                     EEGLFYARTDVGGVYRFDSKAQVWIPLMDFVSQEDVGLYGTESFAIDPKNPGRLYV"
BASE COUNT         2071 a         1140 c         1344 g         2293 t
ORIGIN      
        1 agcgaaaacg gtgtactgga atcccgtcaa tttcgtgcga ttttaaccct tgataagaag
       61 ggcaaaggat ttgaacttga actccagctt cttttagctt ttttacagag gtacgtaacc
      121 acgggacttc tgaatcttca tcaaagcgcg ggtaaacaga tccaatgatt aaaactttca
      181 tgtggacttt tcttccccat taggtttggg aatgacaaag tcttttaagc aggcagggta
      241 aacaaaagcc ctagtccctt tttgaagcgt attagagata tctccaattg tcaaacgaca
      301 aaattcactg atgcgacctt gaagtcgaat ccacccttcc atttttgaat cgagcaatag
      361 aagtccaatt cctgcatcgt cttgtaaaaa gccaagaacg tctgaacctc ttttcttgat
      421 ggccatgcta cgtaatgcgg cttcccaaaa tgcaagctct tctgtgtcgt cttttagagt
      481 agctttgatt ctattaaaat ccatttcgac ataaacaaaa gaacttttat attcatccgt
      541 tcggacaatc tcttcctgga tacgcagttc gaagaactcc gcaggatata gaattaagtc
      601 atatttatgg tttttaatcc cattgaagat aattttcttc atcataatat tagaatttag
      661 tctattttat gagtatgcaa cgattgctta aattaaaaaa agcgctgaag agcagcttta
      721 ttaccacatc tgttgaagtt ttacgctcta caaaagtgcc cataaggtat cgaaccttaa
      781 ctcctgaaga aatcgaaact ctagaaaaaa acggaaatcg atgcgatgac tggtctctag
      841 tttctgtgga acccaatttt tcacctgaga gaatcaatca atccattttt gtaggcgagg
      901 tccgtttacc tgcatttttt ggaacagttt tgcttcctgg agacgtctct tttccaactg
      961 ggatttacta tagcttagtt cataattgta cgattgaaaa tgcgctcgtt catcgagtgg
     1021 cgatgctttc taatgttctt gtgcgttttg gtgctgtgct tcaaaatgta gggtcgatcg
     1081 tttctagtgg gaaaattagc tatcagacag gatccgatat tgtcataggt aatgaaatgg
     1141 gtggtcgtcc gttgtacgtt ttcccagata tcaccgatga gttgattgac gctcaattgc
     1201 ttcaaaaaat gaatgaagaa gtacaaggat cgtttgctga gttgattaat acttggcgtc
     1261 aagaagtctc tttacctttt ggcgttgtag ataaaggcgc tgtgatttca aatacgaata
     1321 ttgtccgcaa tagctggata ggccctcacg cgagaattga aggggcgtct aaaatcagaa
     1381 atacagtcgt gctcagtagc cttgaagaac cttgtaatat ttatgattct gtaattattg
     1441 aaaactccaa tgtgcaagaa ggcgttaaag tacattctgg agcattagtt aaaaattctg
     1501 ttttaatgaa acgagtgaag gttgggaata aggccattat taattcttcg attatttctt
     1561 catgttgtca tattgaagaa gccgaagtaa cgtgctctta tgtagggcct cttactcaga
     1621 tgcatcatca ctcactttta atctctacgc tttggcctat gggcggtggc aatttaggct
     1681 atggtgccaa tgtggggagt aatcatacgg ggcgtaggcc agaccaagaa ttgtatgcgg
     1741 gtcaaggctt gttttttggt ttgggtgtca atatcaaatt cccagcgaat ttaaaagatt
     1801 ctcctttctc gatcattgcg acaggagtca cttgcgagcc acagcgttta atgttcccgt
     1861 ttagtttgat caagaaaggg acgcatagcg atccttcgaa aagctttttg aatgaattaa
     1921 tccctggttg gaactatgcg aaaaacgcat acgcattaga tcggaatttc tataagtata
     1981 gcatccgtgg taaaggtttt gtggacgttt cttttggagc tctatctcaa tcagaattag
     2041 tcaaaaacgt ttttgacgcc taccaaaggc ttcaagtgaa aacgatcaaa gacgtttatt
     2101 taaaagaaga tattcttggt ttagggagta attacctcag agaaacggtg cgtcaaaatg
     2161 cactcaaaac gtaccgtgag tttttagaac gatttgtttt agatgcttgt gtttcggcta
     2221 tggaagcgga ttctagcttg ttaatggaat ctccaaaaga ttttcgaaag agcttttcag
     2281 gggaagtact tcgagagatt acaaaagact gggctttccc agaaaccgtc gccgacttgt
     2341 tgaaaaaata taaggcttta gaaaaagaat ggtttgattg tgtattcaaa ggtctagatc
     2401 gagacgctgt gcgtggtcga gagatttttg atgattacga tgaagttcat ttgccagata
     2461 aggctttcat agactttgaa aagcaacgga tcgaagagtc aaacaaaaga gtggctttgc
     2521 tttctaaaga acttcgctta gaagcctaag gcacaaaatg tttttttggc tagttgtttt
     2581 attaggaata gaattcgttt tccgagtggc tttagaaatt cgggaagcgc gtcttttctc
     2641 tcgcaaaaat cgatttgcgc tctttcgagt gattcctttt ataaacgatt tcgtcccgtt
     2701 tccagagaat cgggatcaaa cagaaataag caatcccttt gttaagtttc atgaagaagt
     2761 acacgaaaaa ctacatcatt caatgacacg gcttttatta aaaggcgttt ttttctttct
     2821 aagtattttg gggatttttt atgtgattgg tcagttgaga atgtctttag tggaagtggt
     2881 gattctcttt catttggctt tattcttttt gcaaattcct taccactatt atatatggca
     2941 gcaagaattt gaagccgatg cttatgcggc taaaaaaaca agcatcactc aggctaaaaa
     3001 gcagtttcgc ttacttgcaa atcaagagat tccttacagc acttttttcg cgttacttta
     3061 tagagaacat cctcctgctg cactacgcct aaaaaaagtc atttctaagt tttcgtaatt
     3121 tttttactgt cgttttttac gaaacgctca tttgaaaagc ctgtccattt tcgctgggat
     3181 tattttcacg agaatcattt cttgaactcg ttacttgaaa tttgttttgc gagattaata
     3241 tcgtaagaca cgcttttttg tgtgatattc gttatcgcga gactaattac ttaaaaaaag
     3301 gttgcgatgc cctatttctt tttttacttt atggctagtg ttctggagga aaaatgagaa
     3361 aatatatcgt cattttattt tatatcttgt gtggatgtca agggaataca acttctgatg
     3421 atgaattagc taaaaataat ccaatggctt tttctgtttt actagcgatg tttgaacagg
     3481 aaagtgcccc tgattttgat tcgaatgttt tagcatatgc aaaaatacct gcctttgacg
     3541 atgattctgc gggcataaat ccattaccat ttaatgatat cgttacttat tttcctaaaa
     3601 ccgcatcgtt aattttgaag cataaaaaat gcgacttcta tataataaag caaaaaaagg
     3661 ataactcaaa ttcttttgat gttcttcatt ctatttcgcc agagggtgct ttgatatatc
     3721 attttgtgcc tacgactcca aagggagatg tttgtaaagc tattcgctct gttgattatt
     3781 gggcgttttt agtcgaggat tgttctcgta ctttaaatca atcaattccg attcgttata
     3841 agacgcatga aagttcttca tggaaatgtg atgatgtaat cccttttaat acccaagagg
     3901 aatggatttc accttctata ttgaaaattt aatgcgtttt ttggggcaaa acggttaatt
     3961 cattttaatt cgatgcatat tggcagaaac gactattcga agtattacgg tgaaatttac
     4021 agaaataatt tgtgaattta attcatactt tttgatgaaa aaactttttc aaagtattcc
     4081 ttaaaaatgc ttattggaaa agttctgtga aaaagagaag tcttaaatta gttggtattt
     4141 attacggagt atttttatgt agaaacgaca aatcgatact ttttacataa ttcagattca
     4201 aatcttaaaa tgataccttt tgttacatat tttgaaattg ttttgaataa attagcatgt
     4261 agcacccttt ttacacagaa aataaaatga ggtgtttatt aagtgccttt ttttgagtat
     4321 tttaagaaca tgaaatacct tacctatact ttatggataa tatttttagc ctcttcgctt
     4381 gtcttttctc gcccagcaaa acctggtgtg atttctgtaa aacaatcaga tagttctagt
     4441 ctttctatcc gtgagtttgg tgatgaacat tatcattata cgactacgga agatgattat
     4501 ttaatcgtta aaaatgaaaa aggtatttat gtttatgttg atgaaaatgg atccccttcc
     4561 aaaattgaag cgaaaaataa agagaaaaga acagctaatg aaaatacctt cttaaaaggg
     4621 atcgatcaaa aaaaggtaaa ggcaaaacat aaaagtaaga ggccgagttc ttatccacag
     4681 caagatagaa agcttaattt taatcgccaa agtcgatcaa tagatgtctc tacaacagta
     4741 ccgcaattat taaaaagacc aacggcaaaa ttatggacag caggagaacg atacattcct
     4801 gttcttttag tatctacttt agataaggcg gcaggagatt ccgccctttt tcatcgtttt
     4861 ctaaatgagc ctggcttttc tgaacaggga aatattggaa gtctcagaga ctattttttg
     4921 atttcttcag atagtttatt taaacctcac tttgatgtat tccctattca gctcaataaa
     4981 aaattaacag attatgtgaa tggtggagat tttttagaag gtcagtttat taaagaggct
     5041 attgatttaa tggttcagaa gccctatttt tctggatcta agtactgcct cagcgaaaca
     5101 acagtcgatg gatttctttt tctttttcca ggaaaagaag aagacgctct aaaaatacat
     5161 gaagattttt ggggacatca atattggatg gaatggaatg gatctaccaa taattacaga
     5221 gcttatcaat ctggaggcta ttcattcaac gcctacgctt ttttagctca aaaagaagat
     5281 ggaaaaccat ctaatataaa tcagatgggc atttttgctc atgagtttag tcatgtcatg
     5341 ggtttatctg atctttatgg gtcagatcaa aatggaaatc ttgttcctgg cccaacccct
     5401 tgggatgtga tgacgcaagg gatgtacaat agtagtggcc gaaaaccacc taaatattct
     5461 gggtttgaac gagaatctat ggggtgggtt accttaactg aattttctgc aaatgaggaa
     5521 atttatactc ttttatcatt agatcaacgg caagcctatt ctgtaactaa tccacaaaat
     5581 aataatgagt attttgtgat agaataccgc cctaaggtag gttttgattc aggaatcaac
     5641 aatacgggtg tattagtttg gtatatagac tatgatagta atgtttggga taacaataac
     5701 cctaatgatg atcctagtca ccagcgtgtg gcagttaaag aggttctttt aggtactcag
     5761 tcaagtaaaa cttcttattc tccttttact ttttatgacg attcctcttc tacagtgcca
     5821 ggcatttttt cttttataaa aggcacaggg gataaggttt gttttactac tagtacaaat
     5881 aaaatgggct ttgtttgtga agaatcttct tcgagtgtga ttgcgtcttc tagctcggtc
     5941 gaagaatctt ctagttcagc gacggtattg atagaaaatc atttaagaaa gtcgaatttt
     6001 aaaattgcgg ttttaggaag gaatcttcag gtttatttgc ctattgtcgg agaaaaagtc
     6061 ttaaaagtca tggatgtgca aggaaatttg gtgcatttgg gtcgttttac aggagacaat
     6121 gaaacgctta ctcttcaaaa taaaggcgtg tattatcttc aaatttcatt aaataatgaa
     6181 cttcttgtct ctagacctgt gaaaattcgt taaaaacggc ttcacgcgtc tcaaaagtaa
     6241 tgtagaactt tgtgacacgc atttttaaag atcaaaaacc aattttagat taaaacagtt
     6301 tttaaaatgt ttgcttcaaa aaaaatgcaa ttcactaata aaaaatgaga gttctttcac
     6361 tacacgtttt atccacaagg tcgggatttc attaattacg cattttattg ttttttttaa
     6421 aaaagccccc cttttttgtt gcgttctaag caacgcttcc ttcgctttat gtcttaataa
     6481 caaggctttt ttaatgaaat aaaataaatt tttattaggg attgaatgcc tttttaagga
     6541 gttaaaatga atttgaaaag tatcatagga atagtcgcta ttttaagcgt atctctcttt
     6601 gctcaagagc catattcttg gggaaacgtg cgtatggatg gtggtggttt tgtttcagct
     6661 gtcatcgctc atccaaagga agaaggtctt ttttacgcaa gaaccgacgt cggtggcgtt
     6721 tatcgctttg attctaaagc tcaagtttgg attcctttaa tggattttgt ttcacaggaa
     6781 gatgtgggtt tatatggaac agaaagtttt gccattgacc ctaaaaatcc aggtcgtctt
     6841 tatgtaat
//
LOCUS       DAOOBB010000097         6813 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_13252_length_6813_cov_5.308819, whole genome shotgun sequence.
ACCESSION   DAOOBB010000097 DAOOBB010000000
VERSION     DAOOBB010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 6813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6813
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13252_length_6813_cov_5.308819"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(579..2759)
                     /locus_tag="PLT31_05600"
     CDS             complement(579..2759)
                     /locus_tag="PLT31_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HPW94649.1"
                     /translation="MRKLFAFIVLAASSVLWAAGSAIITLEMPIGARQLGMGEVGAAI
                     ADDATALYYNPAGLAFGPLADEWRMSYPEKSEKSPYFTKIAARTRPGFFAKSELWTGT
                     SKGIMRFDGERWLNFHSVLLDGNMKIRDVVRVFVGTERGLDEYTRQVKKFNNVKTADD
                     ESRIIEVRIPWDLVIKDTVTALLYEPRTEKLWVGTPNGLFRFDGKGWKTFQSEFGNKR
                     ITALGNQGATLWIGTDNGLFSYRNGSFEQKGKVLPSQYIESLVWSEMRKELYVSVRDA
                     GIARLVPKTNSNDKDRWSLFTEEDGIIDLQPKTLAIDSSGHVWATHKEGLSHFNLRKW
                     EQVRFENNNIHDISVDRKGAIWIATDKGVWRHLPDYATASGRKAELERNPNEKEPVDK
                     DEWNHYHSGNGLSTNRVWAVLPQGNDVWFSTANGMEQYKDADYQLSFFYEKLLPVLNI
                     PDLYHLFGGMTIPLAEWGTLGFFVNFISFGSTVVSDEFTSDDLVAYNSSEIVGGFSYG
                     THIGKDWGLGVSIKLFYSDLSSGASTGEEEATTFGYAFDVGLLKKDLLIKHLNFAAVL
                     ANIGPSVYYVDKSIEDPIPLTWRIGLSYELLSSVDHKLLIATDYNRETVYDDEKGQPE
                     PFYIASWKSIANPELGGHGFTAAKNSLLQGVFNIGAEYTYSNTVALRLGYLYDKTGKR
                     QEVDLGFGFMLSDVLQFDFATIKDVGNNDGVRDGQMRFGLIFKF"
     gene            complement(2752..3318)
                     /locus_tag="PLT31_05605"
     CDS             complement(2752..3318)
                     /locus_tag="PLT31_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94650.1"
                     /translation="MDLSVVLIIVIIIVLSIILAAIGAYVVIHSADEKETVLPQIDVS
                     GQYAVLVKPAKESLSKVKPSSDEIKEWLASQPISEEEKASLLSQWEASLEETIKTIDE
                     GDKNGTVTYRVIIGPRSSPFCNFMNEENYITREQIRNHSEILPPYVLGCDCKLVPKFP
                     WENPGKAGWKPVIPENGMYRVPDWRNIA"
     gene            3385..4521
                     /locus_tag="PLT31_05610"
     CDS             3385..4521
                     /locus_tag="PLT31_05610"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPW94651.1"
                     /translation="MPKVFFLIVLLFSISIWAIPYEVVSIKEGSGEPVKSGQLIQVHF
                     KGWLTDSTLFDDSYSRAEPLEFVLGTGQVISGWDRALVGMKKGEIRRIALPYELAYGD
                     RAIGPIPAKSDLLFEVELLHAEQPLPPDEFPKKEIQWKALDQGVWFYDEKVGKGAVST
                     AGKVLHLHYTGWLSKGFKFASSKDLGKPMEVVLGAGKLIKGWEKGLNQVPEGTVRWLK
                     VAPSMAYGATPLAKIPANSTLVFRVDLITVETDDLVTESMDFFPDTTSLTFKKGAEGL
                     RYAVIKEGEGEPAKVGESVRVHYTGWLMDGTKFDSSKDRAQIFEFPLGGGRVIRGWDL
                     GVEGMLPGEKRVLLVPPGLGYGSRGGGPIPPDATLLFMVEYLGV"
     gene            complement(4510..4965)
                     /locus_tag="PLT31_05615"
     CDS             complement(4510..4965)
                     /locus_tag="PLT31_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HPW94652.1"
                     /translation="MIHFLKYNLIGILNTAITIITVWILHQTLNWNLELSNFLGFVAG
                     GINSYVCNRLWNFKSQNNKREEMIRFLIIFIIAYFINLFVLKFSLYALTELSTFTAFT
                     EWISKWMKPGFVAHIIANVVYVLISFLLYKNWVFKKRDSIQEPPKDLNA"
     gene            5004..6494
                     /locus_tag="PLT31_05620"
     CDS             5004..6494
                     /locus_tag="PLT31_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008775 - acetate CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate CoA transferase"
                     /protein_id="HPW94653.1"
                     /translation="MKSLSQKIMSAVEAADLIKNGECIGISGFTLSGYPKAVPEALAE
                     KAIALHQKGEPFKVTIFSGASTGDDCDGVLARAHAIEKRMPYQSNPSLRQEINQGIIQ
                     YNDEHLGSMGHMVRSGALERPTLALIECIAVTPEGKVYLSSSGGNSATYLECADRVIL
                     EVNSIYGEEYIGLHDTFIPALPPFAKPIPISKVNDRVGSPFIQISPEKIVAIVYTERH
                     DKVNPFTAPDEVSRTIASHILDFIHNEYRQGRLPRGLSYQSGVGNVANAVLAEMAQDS
                     RMEKVNIYTEVIQEAVLPLLQADKLGFASGTALTLSVEAQKQVKKNISRWKDHFILRQ
                     QEISNHPEVIRRLGVISMNTALEMDLFGNVNSSHVCGSSIMNGIGGAADFARHCYLGF
                     FMAPSVAKKGAVSAIVPLVSHVDHTDHDTMIFVTEQGVADLRGLDPVARAKLIISNCA
                     HPSYKAELTDFLNFSLNQSKKGRLPIALDKAFDMHLRLLRTGSMLS"
BASE COUNT         2092 a         1252 c         1392 g         2077 t
ORIGIN      
        1 aaacgcccct ctaactcaac ccagtttttt catgaagaga caataaaacg cccctctaaa
       61 ccaaccctgt ttttttaatg aaaaaacaag cgttaattga attggccaat tcaatagcga
      121 agttttgaat aaaaaaaata accctatcca attaaataac aatagttgca tagcagacga
      181 aaatggattt tactttagag cgactcttgt gtggcacaca acggagcgag aaagtaaaac
      241 catttccgtc aacaagagta acattgagct ttagtgaaaa aaccatttcc gtcaaccaca
      301 gtgatgttca taaaaaaaac gaaataaagt caaccaagcg aagagataac agccatttca
      361 atcaataaca gtggttttga tattaaataa agacgcaatg aaaaaaacat taccgtcaac
      421 aatggtgtta ttcataaaaa acgaaataaa gtcaaccaag cgaggaggta ataacattcc
      481 cgtcaattat atcatttatt aatgagatta aatgaaaaaa ccattgccgt caataatgag
      541 tatatagatt tattcgtaat gaaaataaat gaaaaaaact agaatttaaa aatcaatcca
      601 aatctcattt ggccatcgcg aacgccgtcg ttattaccga catctttaat ggtggcaaaa
      661 tcaaattgaa gaacatcaga gagcataaag ccaaagccta aatcaacttc ttgtctttta
      721 cctgttttat cgtagaggta gcctaagcga agagccactg tatttgaata agtgtattct
      781 gctcctatgt taaaaacgcc ttgtaataaa gaatttttag ctgctgtaaa gccatggcct
      841 cctaattcag ggtttgcaat ggatttccaa gaagcgatat agaatggttc tggctggcct
      901 ttctcatcat cataaactgt ttcacgattg taatctgttg caatgagaag tttatggtcg
      961 acggaagata aaagctcgta agaaagccct attctccatg ttaaaggaat agggtcttcg
     1021 atcgacttat ctacataata aacgctagga ccaatattcg caagcactgc agcgaaatta
     1081 aggtgtttta ttaataaatc ttttttgagt aagcctacat caaaagcgta accaaaagtg
     1141 gtggcttctt cttctcctgt agaggctccg gagcttaagt cagagtaaaa taatttgata
     1201 gagactccaa gaccccagtc ctttccaata tgagtaccgt agctaaaacc gcccacaatt
     1261 tcagaactgt tataagcgac gagatcatcg gaggtaaatt catctgaaac cacggtagag
     1321 ccaaaagaaa taaaattcac aaaaaagcct aacgttcccc attctgcaag aggaatcgtc
     1381 atgccaccaa ataaatggta aagatcagga atgtttaaga caggtaaaag tttttcatag
     1441 aagaacgaga gttggtaatc ggcatctttg tattgttcca tgccattagc tgtactaaac
     1501 caaacatcat ttccttgagg aagaaccgcc caaacgcgat tagtagataa tccgtttcct
     1561 gaatgataat gattccattc gtctttatca acaggttctt tttcatttgg atttcgttcg
     1621 agttctgctt tacggccact cgctgtggcg tagtctggaa ggtgtctcca tacgccttta
     1681 tctgtggcaa tccaaatagc acctttccta tcgactgaaa tgtcatgaat attgttattt
     1741 tcaaaccgaa cttgttccca ctttctgaga ttgaagtgtg ataagccttc tttatgagtt
     1801 gcccaaacat ggccagaact atcaatcgcg agtgtttttg gctgaagatc aataatgcca
     1861 tcttcctctg tgaaaaggct ccagcggtct ttatcgttag aatttgtttt aggtacaaga
     1921 cgtgcaatac ctgcatcgcg aaccgaaaca taaagttctt ttcgcatttc ggaccaaact
     1981 aaagattcaa tatactggct tggtaatact tttccttttt gttcaaagga accattgcgg
     2041 tatgagaata atccattgtc ggtaccgatc catagggttg ctccttgatt accaagagcg
     2101 gtgatgcgtt tgttcccaaa ttcggattga aaagttttcc aacccttacc gtcaaagcga
     2161 aataggccgt ttggcgtgcc tacccagagt ttttctgtac gaggttcata gagaagtgcg
     2221 gtgactgtat ctttgatgac tagatcccat ggaatgcgaa cttctatgat gcgggattca
     2281 tcatcagctg ttttaacatt attgaatttt ttgacttgtc ttgtgtattc atcaagccca
     2341 cgttctgtgc caacaaaaac gcgaaccaca tctcgaattt tcatgttccc atcgagaagg
     2401 acggaatgaa agttaagcca acgttcgcca tcaaaacgca tgatcccttt actggttcct
     2461 gtccatagtt cacttttagc aaaaaagcca ggacgagtac gggcggctat ttttgtgaaa
     2521 taaggactct tctctgattt ctcaggataa gacatgcgcc attcatcagc gagcggccca
     2581 aaggcaagcc ctgcggggtt ataataaaga gcggtagcat catcggcaat tgcagcgccg
     2641 acttcgccca ttcctagttg cctagcacca ataggcattt ccaaagtaat aatagcagag
     2701 cctgcagccc acagaacgct tgatgcagct agaacgataa aagcaaataa tttacgcaat
     2761 gtttctccaa tctggtactc gatacattcc attttctgga atcactggtt tccaaccagc
     2821 cttcccaggg ttttcccaag ggaatttagg aacgagttta caatcgcaac ctaaaacata
     2881 aggcggaaga atttcggagt gatttcgaat ctgttctcga gtgatatagt tttcttcatt
     2941 catgaaatta caaaatggac tacttcgcgg accaatgatt actcggtagg taacggtgcc
     3001 atttttgtcc ccttcatcaa tggttttaat ggtttcttct aaagaagctt cccattgaga
     3061 tagtaatgat gctttttctt cttcagaaat aggttgggag gcaagccact cttttatttc
     3121 atcagaggag ggttttacct tgcttaaaga ttcttttgct ggttttacga gtacagcgta
     3181 ttgacctgag acatcaattt gtggtaagac agtttctttt tcatcggccg aatgaataac
     3241 aacataggca ccgatggcgg cgagaatgat tgataacacg ataatgatga caataattaa
     3301 aacaacagaa agatccataa tcattcatcc taagtagagt gactaataaa ctagtatttt
     3361 ctagtttatt agacaggaga ttttatgcca aaagtgtttt ttttgattgt cttgttgttt
     3421 tcgataagta tatgggctat cccatacgaa gttgtttcca tcaaagaggg tagcggtgaa
     3481 cctgtaaaat cgggtcagct gattcaagtc cattttaagg gttggcttac agatagtacg
     3541 ctatttgatg attcctatag tcgtgcagaa cctctggaat ttgttttagg tacaggccaa
     3601 gttatttcag gatgggatcg tgctcttgtt ggcatgaaaa aaggtgaaat aaggcgtatt
     3661 gctttgccat acgaactggc atacggagat cgggcgatcg gtccaattcc tgcgaagtct
     3721 gatttacttt ttgaagtaga acttttgcat gcggaacagc ctctccctcc agatgaattt
     3781 cctaaaaaag aaattcaatg gaaagctcta gatcagggag tctggtttta tgacgaaaag
     3841 gttggaaagg gtgcagtaag tactgcgggt aaagtactcc atcttcatta tactggttgg
     3901 ctttcaaaag gttttaaatt tgcaagctct aaagatttag gtaaaccaat ggaagttgtt
     3961 ttaggtgctg gaaagctaat caagggttgg gaaaaaggct taaatcaagt tccagaagga
     4021 actgttcgtt ggttaaaagt agctccttct atggcttatg gtgctactcc tttagcaaaa
     4081 atcccagcca attctacgct tgtatttcga gtggatttaa taactgtaga aacagatgat
     4141 ttagtcacag aatctatgga ctttttccca gatacaactt ctttaacctt taagaaaggg
     4201 gctgaagggc ttcgctatgc tgtgataaaa gagggcgaag gggaacctgc aaaagtaggc
     4261 gagtctgtca gagttcatta tacaggctgg cttatggatg gcaccaaatt tgatagttca
     4321 aaagacagag ctcaaatatt cgaattccct ctcggtggcg gtcgcgtgat tcgtggttgg
     4381 gatcttggtg ttgaaggcat gcttcctggg gaaaaacgtg ttttactcgt ccctccagga
     4441 cttggctatg gaagtcgtgg tggtggccca attcctccag atgcaacgct cttgtttatg
     4501 gttgaatatt taggcgttta gatctttagg gggctcttga atagagtccc tttttttgaa
     4561 aacccagttt ttatataaaa gaaaactaat taatacgtaa acgacattgg caataatatg
     4621 agccacaaat cctggtttca tccacttgga aatccattct gtgaaggctg taaaagtact
     4681 taattcggtt aacgcataaa gagaaaattt aaggacgaat aagttgataa aataagcaat
     4741 gatgaaaata attagaaatc gaatcatttc ttctctttta ttattttgac tcttaaaatt
     4801 ccacaagcga ttgcatacgt aagaattgat accgccagcg acaaagccta agaaattgga
     4861 gagttccaaa ttccaattta aggtttgatg taaaatccaa acggtaatta tggttatggc
     4921 ggtatttaaa atgcctatga gattatattt caggaagtgt atcacaagtt cgaaaataat
     4981 aaaaaaactt atctttggtt atcatgaaga gtctatcaca aaagattatg agtgctgtag
     5041 aagcagcgga tttaataaaa aatggtgaat gtattggtat ttctggtttt acattatctg
     5101 gttatccaaa agcggtacct gaggctttag ctgaaaaagc aattgcctta caccagaaag
     5161 gagaaccttt taaggtaact atttttagtg gtgcttctac tggtgatgat tgtgatggcg
     5221 tcttagccag ggctcatgcg attgaaaagc gtatgcctta tcaatcgaat ccctctctta
     5281 ggcaagaaat taatcaaggt ataatccaat ataacgatga acatttaggt tccatgggac
     5341 acatggtgcg ttcgggcgct cttgaacggc ctacacttgc cttaattgaa tgcattgctg
     5401 tgaccccaga agggaaggtg tatctttctt cttcgggcgg caattcagcc acatatcttg
     5461 aatgtgcaga tcgagtgatt ttagaagtta actctattta cggtgaagaa tacattggcc
     5521 ttcatgatac ttttattcct gcattacctc cctttgcaaa gcccattccc atatcaaaag
     5581 taaatgatcg agtcggttct cccttcattc aaatttcacc tgaaaaaatt gtagccattg
     5641 tttacacaga aaggcatgat aaagtaaatc cttttaccgc accagatgag gtgtcaagaa
     5701 caattgcttc tcatatttta gattttatcc ataatgaata tcgccaagga cgtctgccaa
     5761 gaggcttatc ttaccaaagc ggagtgggga atgtggcgaa cgctgttctt gctgaaatgg
     5821 ctcaagattc tcgtatggaa aaagtaaata tttatactga ggtgattcaa gaagcggttc
     5881 ttccgctttt acaagcagat aaattgggct ttgcttctgg aacagcatta actctttctg
     5941 tagaagcaca gaagcaagtc aagaaaaata tttctcgatg gaaagatcat tttattttaa
     6001 ggcagcaaga aatttcaaat catccagaag tgattcgtcg tttaggtgtt atttctatga
     6061 atacggctct tgaaatggat ttgtttggta acgtcaatag ttctcatgtt tgtggctctt
     6121 ctattatgaa tggcattggt ggtgcggcgg attttgcaag acattgttac ttaggttttt
     6181 ttatggcgcc ttctgtagca aaaaaaggag ctgtaagtgc gattgtacca ttagtaagtc
     6241 atgtggatca taccgatcac gatactatga tttttgtaac agagcaaggg gttgctgatt
     6301 taagaggatt ggatccagtt gctcgagcga aattgatcat ttcaaattgt gctcatccta
     6361 gctataaagc ggaattaacg gattttttga attttagttt aaatcagtcg aaaaaagggc
     6421 gtcttcctat tgcattagac aaggcgtttg atatgcattt gcgtttatta cgtactggaa
     6481 gcatgctttc ttagtgcttt ttgaatccac ttttattgaa ctaaagtttt attttgttat
     6541 attctttttt gaattatttt tcaatagtgt tttttgcttc tttatcgttg ttactggaac
     6601 cttttaatga atttaatatc cttaaaaagc ttaggctatt ttatctttgt cactgaatta
     6661 tttcttacaa ataaggaaag ataaaaatgg ctataaagca agaattactg gatcagttag
     6721 aagcgcgtcg taatgaggca ctaaccagcg gttgtggtga ggataaaatt gctgcccgtc
     6781 atgcaaaagg tttaatgact gcttatgaaa ata
//
LOCUS       DAOOBB010000098         6811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_13257_length_6811_cov_4.687981, whole genome shotgun sequence.
ACCESSION   DAOOBB010000098 DAOOBB010000000
VERSION     DAOOBB010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 6811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6811
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13257_length_6811_cov_4.687981"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="PLT31_05625"
     CDS             <1..514
                     /locus_tag="PLT31_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94654.1"
                     /translation="NKPFSLRAFANPYKYTEDLDFYWYRDKKRLAKSVIYTLDTPFVE
                     SQVPNRLKAMDSENNELFFDFKVHLNNPPQMLFKTTPNDQDTLYGSTSSAFLFSWSSS
                     DKDRNDSLNHYIKIDKTTYFTGQLTSIQQSGFKPGVHSFSIWVIDSFGDADTLVTKTF
                     VVIDSLGVPK"
     gene            511..1794
                     /locus_tag="PLT31_05630"
     CDS             511..1794
                     /locus_tag="PLT31_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94655.1"
                     /translation="MKKLFFSYFIFAFILILVSCNAGENALFDGSEYEGTISIDAHIT
                     HSLDTSSLRVKTDTLIPGDSVIFLVSVYPSKAIRLQNYFWKINEKKRGAEFSFRSAFE
                     KAGKQTAVFHLVDYYGDTLTDTLIIWVSNPPQLNDSVFIPAKASQHISSEKNVSFVWD
                     ASDPDSNDLLKHHFRLYNNDTSFVDTILYEPFFNYPFQLPPLQQFFWSIETFDSFGLK
                     APQKIESSFFTKSLSPEEGAVIIPISSKNFSLISKLDYIITPKEGQVLPLYLLEKEKP
                     SLDKGLLHLSFLKEGTYEIDLFHSQYPDFKISPVSFSIKKEDVLILDSILLTDSISPF
                     LFNLNSTNDTIDNQDSLYFYVSDGGIPLTKNNIQVYFDSELFQSWTYSDSIVWISIPK
                     EKQNDFWHLLSFKITDFSTNVKKHTFYLSPASFAE"
     gene            1903..3378
                     /locus_tag="PLT31_05635"
     CDS             1903..3378
                     /locus_tag="PLT31_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPW94656.1"
                     /translation="MRFYNVHATPAHSARFGKQTHLQLLDPYNTVPRKPFYRSLLILF
                     TCMIFIGCGEDSLYSKSDTSQITQNIYVVPEFFSGNLLTNYSAARVQTIDLGQTIKFW
                     ASFYINDEQISNDQITNYPFNIEWTLNDESYNIPYFIKTFNETGSFDAILTTTDLFKD
                     TLRDTVTIQVNQPINIKAIYPADGYNLLDVTDSAGITLHWEFSGQDPWEKVSCLFYAS
                     RIKDLTWNNLIDTVKCDEPLTLLGPFEQENSISYSDTSIAFYWGIKIITDKGTYPSQR
                     DSTGINSFRTRLKDNSLSIIEVPIKYLQIGQNTSPYTRVDLISASGDTIQSKFTLLTQ
                     DTFYFKNIPQQNGLFIKATNILLPEYITQSISMDIISGSYNVIPTILFTDSIAPERAP
                     LLQTLSPEDSIVFKIKDLGSGVNPKSIRVILNHDTLKHVFSDSVLKFAAPNLFQCQEP
                     CILNVEVTDFASNFSSLIHWTLSSKKDSLVLEGPFPLRELQ"
     gene            3375..3998
                     /locus_tag="PLT31_05640"
     CDS             3375..3998
                     /locus_tag="PLT31_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94657.1"
                     /translation="MIKFLFYFLLFSSLLFAEDSIQFQLSSFPPHADIYLGSPPNSFS
                     QKAPFSTPATIRLPVDSTQVLVTLFKKDYSDTTIDIKLIPNERGEAFLTIMLKQELNE
                     SILEQQKKDLFRRAKREYGHMILLGTIPASVATIINALIMQYHISEAEARSTEIDKIL
                     IRQNEHFKSLKDDFHHHRHHAQNFKRGTLISAVATSLFLIVGCTLSF"
     gene            4005..5510
                     /locus_tag="PLT31_05645"
     CDS             4005..5510
                     /locus_tag="PLT31_05645"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HPW94658.1"
                     /translation="MKYSSFIFVLFFLSSFLWAEEPHGPTVYVELKSGTKQKAQYLGS
                     IKDTVILGGYIQNSYTKIKLPKSAFKSILDSTGQALFSSEKKLPSEDLISSTEIIQSD
                     TVKKEEPISIKDKTILIPLKRRGIDSLLAERLLHLTSELLIEQNESVEVISSSFYSEC
                     KELECIRKLSHEQGARALWNGDILPAKSQDSLILKLERTLFPQKTPEQIQITIAAKTP
                     LQDLLKEEKWISFIQNKVMNKKKPKSYVYVETDPEGASVSKQGKASICKTPCTFATLD
                     TGKILIETYWHVDQHLWGNQENIQLIPDDTVKVSLRLKRIQPEIEITTNPFGALIFEG
                     NDSLSIKKRSLGKTPKRFKTFEPGLAYIRLWKEGYQDTVIEFNVNAQEKTLLSVDLTP
                     LKNFEAIEKQQKWIKAKNTRFWGLTITGSAIAPIITGALFTYLSHKNYEEAKEIKNSL
                     MQPSIQDGDHFQRLVEKNKDLVEKGDRKLVIGGSLFGLGALMIGFGLTILF"
     gene            5579..>6811
                     /locus_tag="PLT31_05650"
     CDS             5579..>6811
                     /locus_tag="PLT31_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94659.1"
                     /translation="MQRIAFILILAIAIATPAADFERSNWRTQLYFQVEPYSLVQFSG
                     DGEFLNESIDTELWTIPLSLGFLWSPLFTPAFKLDVPFTIWLGIEAASFQFKEIQSYP
                     EYKFPEGSVERDGPSRNEILSFYSVNPAVLGGFSFNIAGDFDLRVLGGFGVQRYSFKD
                     EFLDVSREKASYHKYYFVSGALEYRIAEIFEDGDLKIGINVRKEFEKRENVIATNSTK
                     NEEQVGIPNGYSGVEFESITNKLPVRVALELSLEFGKESRRDRKVRFALFDRDSVLRK
                     NSEVKDTLSDWDCMAIERDYKLFLEDGDLPDVSASFTKVQFADVLESYLAFCKPADLN
                     TKEHLYSALDSSKVNLKQYQVKQEDSRYQQVMASNDLEMMQMFLQYYPETPHREAIEA
                     KIKVISDYQVFKAAQKENS"
BASE COUNT         2184 a         1326 c         1068 g         2233 t
ORIGIN      
        1 caacaaacca ttttcattac gagcatttgc aaatccctat aagtatacag aagatcttga
       61 tttttattgg tatcgagaca aaaaacgttt agcaaagtcg gttatatata cactagacac
      121 tccttttgtt gaaagtcaag ttcccaatcg attaaaagca atggattctg aaaacaatga
      181 gctatttttt gattttaagg ttcatttaaa taatccacct caaatgcttt ttaaaacaac
      241 tcctaatgat caagatacgc tatatggctc tacatcttca gcctttttat tttcttggtc
      301 ttcttcggat aaagatcgaa atgattctct aaaccattat attaaaattg ataagacaac
      361 ttattttaca gggcaactca cttcaattca acaatctggt ttcaaaccag gagttcattc
      421 tttttccatt tgggtcattg attcttttgg agatgctgat actcttgtca caaaaacctt
      481 tgtcgtaatc gattctttag gagtgccaaa atgaaaaagc tattctttag ttattttatc
      541 ttcgcattca tattaattct cgtaagctgc aatgcaggag aaaacgctct ttttgatggc
      601 tcggaatacg aagggactat ttctatagac gctcatatca ctcactccct tgatacatca
      661 tctcttcgcg taaaaacaga cactctcatc ccaggagaca gtgttatttt tttagtctcg
      721 gtatacccgt ctaaagccat ccgtctacaa aattattttt ggaaaataaa tgaaaagaaa
      781 cgaggcgcag aatttagctt ccgttctgcc tttgaaaaag cgggtaaaca aaccgccgtt
      841 ttccatctcg tggattatta tggagatacg ttaacagata ctcttattat ttgggtatct
      901 aatccacccc aactcaatga cagtgtattt attcctgcaa aagcttctca acatatatca
      961 tctgaaaaaa atgtctcttt tgtttgggat gcgagtgacc ccgattctaa cgatcttcta
     1021 aagcatcatt ttcgtttgta caataacgat acatcttttg tggataccat tttatacgag
     1081 ccttttttca attacccatt tcaacttcct cccctacaac aatttttttg gagtattgaa
     1141 acatttgatt cctttggctt aaaagcgcct cagaagatag agtcatcttt ttttacaaag
     1201 agcctatctc ctgaagaagg ggctgtgatc attcctattt catccaaaaa tttttcacta
     1261 attagtaaac tcgattatat aatcactcct aaagaagggc aagttcttcc tctttacctt
     1321 ttagagaaag aaaaaccttc tttagataaa ggtcttcttc atctatcttt cttaaaagag
     1381 ggaacgtacg aaatcgattt attccactcc caataccccg attttaagat tagccccgtt
     1441 tctttttcta taaaaaaaga agacgttctc attttagatt ctattctctt aacagattca
     1501 atcagcccat ttcttttcaa tctcaattca acaaatgata ccatagataa tcaagattca
     1561 ctttattttt atgtttctga tggaggcatt ccactaacta aaaacaacat tcaagtctat
     1621 ttcgatagcg agttattcca atcatggaca tactctgatt ccatagtgtg gatatccatt
     1681 ccaaaagaaa aacaaaatga tttttggcat ttacttagtt ttaaaatcac tgatttttca
     1741 acgaatgtaa aaaaacatac tttttatctt tctcctgcaa gctttgccga gtaaaatatg
     1801 atttgctttt ttgataaaca tatcttgacg cttcgcgcta gtacgatatt caacaagaga
     1861 aatatcatcc atattttcca tttttgtgtt taccacttgt ttatgcgatt ctacaatgtc
     1921 catgctacgc ctgcgcactc tgctcgtttt ggcaaacaaa cgcacctgca attgctcgat
     1981 ccatacaata ctgttcctcg taaaccattt tatagaagtc tccttatttt attcacatgc
     2041 atgattttta tcgggtgtgg agaagattcc ctttattcta aatccgacac gagtcaaatc
     2101 acacaaaaca tttatgtagt tcctgaattt ttttctggaa atttattgac caattattct
     2161 gcagctcgtg ttcagacaat tgatcttggc caaacaatta aattttgggc aagtttttac
     2221 attaatgatg aacaaatcag taacgatcaa atcaccaatt atccttttaa tatcgaatgg
     2281 actttaaatg atgagtctta taatattccc tactttataa aaacatttaa tgaaacaggt
     2341 tctttcgacg cgattctcac aaccactgat ttgtttaaag atactcttag agacactgtc
     2401 accattcaag taaatcaacc tattaatatt aaagccattt atccagctga tggttataac
     2461 ttattagacg tcactgattc agcagggatt actcttcatt gggaattttc tggacaagac
     2521 ccttgggaaa aagtttcatg ccttttttac gcttctcgca taaaagacct gacctggaat
     2581 aatcttatag atactgtgaa atgcgatgag cctttaactc ttcttgggcc ttttgaacaa
     2641 gaaaattcga tatcttatag tgatacctct attgcttttt attggggaat aaagattata
     2701 accgataagg gaacataccc ttctcaacga gattccacag gcattaattc ttttagaacc
     2761 cgattaaaag ataattccct ttccattata gaagtcccta taaagtatct tcaaattggt
     2821 caaaatacta gcccctatac tcgtgtagat ttaatttctg cctcaggaga tacgatacaa
     2881 agtaaattca ctttactcac tcaagataca ttttatttca aaaacattcc acagcaaaac
     2941 ggtttattca tcaaagccac caatattctt ctacctgaat atataacaca atccatttct
     3001 atggatatca tttcaggctc ctataatgta atacccacaa ttctatttac tgattccata
     3061 gctcctgaac gggctcctct tttacaaacc ctttctccag aagattctat tgtatttaaa
     3121 atcaaagatc tcgggtctgg tgtcaatcca aaatctataa gagttatttt aaatcatgac
     3181 accttaaaac atgtgttttc tgattcagtt ctaaagtttg ctgctccaaa tctcttccaa
     3241 tgtcaagagc cttgcatact gaatgtagaa gtcacagact ttgcctctaa tttttcgagt
     3301 ttaattcatt ggactctttc ttcaaaaaaa gattcattag tactagaagg gccttttcct
     3361 ctaagggaat tacaatgata aaatttcttt tttattttct gctctttagt tctctccttt
     3421 ttgctgaaga ctctattcaa tttcagttat ctagttttcc tcctcacgca gatatatatc
     3481 tcggttctcc tccaaactct ttttctcaaa aagcaccttt ctctacccca gccaccattc
     3541 gtttacctgt agacagtact caagtacttg ttaccttatt taaaaaagat tactctgata
     3601 caaccattga cataaaacta atacccaatg aacgtggcga agctttttta acgattatgt
     3661 taaagcaaga attaaatgaa agtatcttag aacagcaaaa aaaagatctc tttagacgag
     3721 caaaaagaga atatggtcat atgattcttt taggtactat tcctgcttct gtagccacga
     3781 tcatcaatgc tcttattatg caatatcata ttagcgaagc agaagctcgc tctacagaaa
     3841 ttgataaaat tttgattcga caaaacgaac actttaagtc attaaaagat gattttcacc
     3901 atcacagaca tcatgcccaa aattttaaac gagggacttt gatttcagca gtcgcgactt
     3961 ccttattttt aattgttgga tgcaccttat ccttttgagg acttatgaaa tattcttcat
     4021 ttatttttgt tctctttttt ctctcttcct ttctttgggc ggaagaacct catggcccta
     4081 ctgtatacgt agagctaaag tcagggacca aacaaaaagc ccaataccta ggatccatta
     4141 aagatacggt gattcttggc ggatacattc aaaattccta taccaaaatc aaattaccta
     4201 aatcggcttt caaaagtatt ctagactcta ctggacaagc gctattttct tctgaaaaga
     4261 aattgccttc tgaagatctt atttcatcaa cagaaataat tcaatccgat accgtaaaaa
     4321 aagaagagcc tatttcaata aaagataaga ccattttaat tcctctcaaa cgtcgaggga
     4381 ttgattctct tttagcagaa cgccttttgc acttgacttc cgaattactt attgaacaaa
     4441 atgaatccgt agaagtaata tcttcgtctt tctattcgga atgcaaagaa ctcgaatgta
     4501 ttcgaaaatt aagtcatgaa caaggggcta gggccttatg gaatggtgat attcttccag
     4561 caaaatcaca agacagcctg atattaaaac tagaacgaac cctatttcct caaaaaacac
     4621 cagaacaaat tcaaatcacc attgcagcta aaacgccctt acaagattta ttaaaagaag
     4681 aaaaatggat ctctttcatt caaaacaaag taatgaataa gaaaaagccc aaaagttatg
     4741 tttatgtgga aaccgatcca gaaggagcta gcgtgagtaa acaaggaaag gcttctattt
     4801 gtaaaacgcc ttgtactttt gccactcttg atactggtaa aattttaatt gaaacttatt
     4861 ggcatgtaga tcagcatctt tggggaaacc aagaaaacat tcaactaata ccagatgaca
     4921 cagttaaagt ctcactgcga ttaaaacgaa tccaaccaga aattgaaatc acgacaaatc
     4981 cttttggagc tcttattttt gaaggcaatg attctctttc cattaaaaaa agatctctcg
     5041 gaaaaacacc taaaagattc aaaacttttg aaccaggcct tgcatacatc cgtctctgga
     5101 aagaaggtta tcaagacacg gtgatcgaat ttaatgtgaa tgctcaagag aaaactcttc
     5161 tttctgttga tttaactccc ctaaaaaact ttgaagcaat agaaaaacag cagaaatgga
     5221 tcaaagccaa aaacactcgt ttttggggac ttaccattac aggaagtgcg atagccccaa
     5281 tcattactgg agcccttttt acctatttaa gccataaaaa ttatgaagaa gcaaaagaaa
     5341 tcaaaaattc attgatgcaa ccgagtattc aggatggaga tcatttccag cgtcttgtag
     5401 aaaaaaacaa ggatctagta gaaaaaggcg atcggaaact ggttattggg ggttctttat
     5461 tcggccttgg tgcattaatg atcggatttg gcctaactat cctattttaa gccattattt
     5521 gattaaaaat tgttttttat acgttttttc cctttaacaa aagttaaatt tagcaacgat
     5581 gcaaagaatt gcctttatat taattttagc gattgccata gcgactcccg cagctgactt
     5641 tgagaggtca aactggcgta ctcaactgta ctttcaggta gaaccatact ctttagttca
     5701 attttctggt gatggggagt tcttaaatga gtccattgat acagagttat ggactattcc
     5761 attatctctt ggatttctct ggagtccttt gtttacacca gcatttaagc ttgatgttcc
     5821 ttttactatt tggctcggaa ttgaagcggc ctcttttcag tttaaggaaa ttcagtctta
     5881 tccagaatat aaattccctg agggttctgt agaaagggac ggtccatctc gaaatgaaat
     5941 tctttcattt tactccgtca atccagcggt gctcggtggt ttttcattca atattgcagg
     6001 tgattttgac ttaagagttt taggtggctt tggcgtacaa cgttactcat tcaaagatga
     6061 attccttgat gtgagccgag aaaaagcttc ttaccacaag tattattttg tttctggtgc
     6121 tcttgaatat cgtatagccg aaattttcga agacggcgac ctcaagatcg gaatcaatgt
     6181 ccgtaaggaa tttgaaaaac gagaaaatgt tattgctaca aattctacta aaaatgaaga
     6241 acaagtaggt attcctaacg gctattctgg tgttgaattt gagtccatta caaacaaatt
     6301 acctgttcgt gtggctctag aattatctct tgaatttgga aaagaaagcc gtcgtgatcg
     6361 aaaagtgcgt tttgcattat ttgatagaga ttctgtttta cgtaagaata gtgaagtaaa
     6421 agacactctt tctgactggg attgtatggc cattgaaaga gattacaagt tattccttga
     6481 agatggtgat ttacctgatg ttagtgctag ttttactaag gtacagtttg ccgatgtgtt
     6541 agaaagctat ttagcttttt gcaaaccagc agatcttaat acaaaagaac acttatattc
     6601 agctcttgat agtagtaaag tcaatctaaa acagtatcaa gtcaaacaag aagattctcg
     6661 ttatcaacag gtgatggcat cgaacgatct tgaaatgatg caaatgttct tacaatacta
     6721 tccagaaacg cctcaccgcg aagcgatcga agcgaaaata aaagttattt ctgattatca
     6781 agtttttaaa gccgcccaaa aagaaaattc a
//
LOCUS       DAOOBB010000099         6785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_13308_length_6785_cov_5.754829, whole genome shotgun sequence.
ACCESSION   DAOOBB010000099 DAOOBB010000000
VERSION     DAOOBB010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 6785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6785
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13308_length_6785_cov_5.754829"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="PLT31_05655"
     CDS             <1..1029
                     /locus_tag="PLT31_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94660.1"
                     /translation="NGAHPKIVLVNPSDSSIIELAEGDELWHPTLWVENIVEPDTGFQ
                     LDPDSAGIYYTANGSSAAEKYRYKMQLLWQYKDSTSIIILGSSRIFYTINPNFFVKPS
                     SALNMANDNNTLNGSYFLFKTYIFPHVKNLKYLVIGTDIDRLKFLDSFWEDEAFTYPG
                     YVYDRSHNYWVDEYPEKLFLFTSESPSNKQHIKDYILTHYGFSSHSTNGWNDSIPSIH
                     GDSTWSSSDMDAFNKNIELLREIAQIGEQNNFYVIALETPQNPAYQNTGAYGKNGILR
                     SKAPDMIKTIQDISKDYPHFIFMDENKMGNHDYTSEMASNDDHLAHLGAVKISHRVDS
                     LIQFLESK"
     gene            1235..2212
                     /locus_tag="PLT31_05660"
     CDS             1235..2212
                     /locus_tag="PLT31_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HPW94661.1"
                     /translation="MGLKYFSIITTAALMASFYACSSDVNGAHENEITSSSESTNYYS
                     FEIASSSNVEPLSSFDSVSSSSAMSSSSAEQSSSSSNVPVDPNILWSDEFNGTAIDTS
                     KWTFEIGDHGWGNNEWEYYTDRAENAYVKDGILHIRANKEIFESAKYTSARMITKGKF
                     SFFYGTIEARIALPVGKGIWPAFWMLGDNIDEVSWPACGEIDIIEAINDESVVYGTHH
                     WAHDGTHANYGNSTRDYYGTSYPMDISEFHTYKMTWDKNAIAMYVDDFKYQEIAIAEA
                     GDMDTFHKKFFFILNVAVAGTWPGFEVDDTQFPTEMLVDYIRVYKNTSN"
     gene            2248..3078
                     /locus_tag="PLT31_05665"
     CDS             2248..3078
                     /locus_tag="PLT31_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94662.1"
                     /translation="MKNSMKILCLILFIAIFSFATLSKETRTNFVSSCIDGGSSSVFC
                     ECVFKKMEQKYTQSQIDAIELKMQSGQSDLGYSNFIKSTSKECNAKTKSGTSLGAMAA
                     NENKSNQKTQSTLSSGDFEAMENMDIQAAFAQGMMAAMIESPQFKNMFIAQCDTELIP
                     YFGKKQAKSTCECSYEKILSSGYIQKILNSINEEGKLEDSLTVELFMPCIPKTYTPQM
                     EQFLMSSCQTKATKNICNCIVKDIKKSFTLEQLLRKTLTDPTFIESYATGAAMRCKNN
                     "
     gene            3116..4732
                     /locus_tag="PLT31_05670"
     CDS             3116..4732
                     /locus_tag="PLT31_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94663.1"
                     /translation="MCVWKNILIVTAVAASLATSASISIDPTATAQKIVGFGGGVVYY
                     QGWFVALSPESKEALYDTAFTGLNLSLLRLGNWMQTDTNDISADVEIVKAAKDRLGDH
                     LKIQMSSWTAPANLKPSGDENGSPTQSGNDKYPRKSATLNTSTSDPYGKYAYTDFANW
                     WKKSYQAYTDAGINIDYISLQNEPDMFAAYAETLFDETENDSTAGYAQALNAVYDAMK
                     TLPNPPKILGPEPLGIGYNNFQKYANALDASKLDGYAYHLYHAGDGNDNSLNNYLNPE
                     NYRKPMSQIANSFYSESKPIIMTEFCTMEENGKENYMVGLAHIMQVGFTAGKLGGYIA
                     WELMWGDGVGQLIGVCTKGWGSCTEDAITISPEYHAMRHYSKFVNPGWSVVNATVTDA
                     TNLYVVAFRSADEDSLTVVAVNSNSAATSLTSLDVKNYKAIDAVQSVENGEKSKKIAT
                     ANSYTLPAKSITTFVFSGESNRVIPAEIHLNPNATSPISQVFDLQGNLLWTGKLTNAE
                     LTDGVLHLQNLNAGLYLVRHGSKTITAVKK"
     gene            complement(4866..5726)
                     /locus_tag="PLT31_05675"
     CDS             complement(4866..5726)
                     /locus_tag="PLT31_05675"
                     /inference="COORDINATES: protein motif:HMM:NF024740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HPW94664.1"
                     /translation="MEPLELEIHKKYIERLNVSFQTIETKINTLEQLAFKYDLFFLDA
                     FGTLYNQNHYVYPGALEMVRYLRQKGKTIRLLTNAASQPVVKLLKDLWLMGFDFLHEE
                     VVTSGDLLTLENQKQNIQEAFYLGRETGVAFLEAAGITFSENPQDPVVILSNVVREVE
                     LREKAIEILKRPNAKLIVLNPDPWAPSIDGSRKPVSGAFAYYLIQQTNAEVIYLGKPF
                     PTIFSETLKKIPKSSKAIMIGDSLGTDVLGACAAGIDSALILGRNTPKESVAEDEAKL
                     GIRPTYYLNI"
     gene            complement(5730..>6785)
                     /locus_tag="PLT31_05680"
     CDS             complement(5730..>6785)
                     /locus_tag="PLT31_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94665.1"
                     /translation="SQRTSFTITPSSLKIDSSYNRLVYFDFPSPVPMQPYLTSLNDFS
                     YGDYGSAILYQSNGDAIFISMKDQMAPVIQETFFKSYRGRGDDTLKIVFSEPSFDNQN
                     CGLIIEYKTASGDDVSLWQYSSYYWNVLHREAFFILDKELFTETTMTPKDSIRIKASC
                     IMDSSRNQIPESTPFVALKGSYPIEIFTVNKATYFENSKKKNGRVFELDFLPFESLYP
                     NDTAVGVALDVGGPEFETVIREELRLRYPSLEIGKDEKIDISKIKIVIKMCLFTSLGT
                     YVASDEVSFKGSDSRLENRGKRVFFRWNFLSDDGRFVQTGVYLVDVDVRISYNGHAVY
                     QTKDKENRMQSWGVLRR"
BASE COUNT         2197 a         1398 c         1287 g         1903 t
ORIGIN      
        1 aacggagctc atcccaaaat tgtacttgta aacccaagtg acagtagtat catcgaactc
       61 gctgaaggtg atgaactatg gcaccctact ctatgggtag aaaatattgt ggaaccagat
      121 acagggtttc aactagatcc agatagtgca ggtatttatt acacagcaaa tggcagcagt
      181 gctgcggaaa aataccgtta caaaatgcaa ctcctatggc aatataagga ttccacaagc
      241 attatcattc ttggatcctc tcgcatattc tataccatta atcctaactt ttttgtaaag
      301 ccatcttccg ccctcaatat ggcaaacgat aataacacgc ttaatggttc gtatttttta
      361 tttaaaacgt atatttttcc tcatgtcaaa aatctcaaat atcttgtgat cggaaccgat
      421 atcgatcgac ttaaattcct tgatagtttt tgggaagacg aagcctttac ttaccctggc
      481 tacgtatacg atagatccca taattactgg gttgatgaat atccagaaaa attatttctc
      541 tttacaagtg aatccccaag taataaacaa cacataaaag actatattct tacacactat
      601 ggattctcat ctcattcaac taatggatgg aacgattcca ttccatccat acatggagat
      661 agtacctgga gttcatcaga catggatgcg tttaacaaaa acattgaact attgcgagaa
      721 atcgcacaga ttggagaaca aaataatttt tacgtcatcg ctcttgaaac cccacaaaat
      781 ccagcttatc aaaatactgg agcttacgga aagaacggca tattgagaag caaagctcct
      841 gatatgatta aaaccattca agatatctcc aaagactatc ctcattttat tttcatggat
      901 gaaaacaaaa tgggtaatca cgattacaca agtgaaatgg caagcaacga tgaccactta
      961 gcgcatttag gagccgtgaa aatatctcac cgtgttgatt cattgatcca gtttctagaa
     1021 agcaaatagt agcaagaata cttaaaagca agtgcaacct taaatgtact tgctttttgt
     1081 aatgttttgt attataaaca acgttaaata gaggatctat gacaacgata gttttgcaaa
     1141 ttcaaataga agaagacctt aaaaatgagg ccgatttcga acactaatat aaagatttac
     1201 acttcattgc tttataaaat cgtattttta aattatgggt cttaagtatt tcagcattat
     1261 taccaccgca gcacttatgg cgagctttta cgcctgttct tctgatgtaa acggtgctca
     1321 cgaaaatgaa attactagca gttccgaatc cacaaattat tacagtttcg aaatcgcgag
     1381 cagttccaat gtcgaaccat taagtagctt tgattctgtg agcagctcat ccgcgatgtc
     1441 atcatcgagt gcagaacaat caagcagttc ctcaaatgtt cccgttgatc caaatattct
     1501 ctggtctgac gaatttaacg gcaccgccat tgatacaagc aaatggactt ttgaaatcgg
     1561 cgatcacggc tggggcaaca acgaatggga atactatacc gatcgtgcag aaaacgccta
     1621 cgtcaaagac ggaatccttc acattcgtgc taataaagaa atctttgaat cagcaaaata
     1681 cacgtcggcc cgcatgatta ccaagggcaa gttcagtttt ttctacggca ccatcgaagc
     1741 ccgtattgct cttcccgtgg gtaaaggcat ctggcctgcc ttctggatgc tcggcgataa
     1801 catcgacgag gtcagctggc ctgcctgcgg tgaaattgac attatcgaag ccatcaacga
     1861 cgagagcgtt gtctacggca cacaccactg ggctcacgac ggtacacacg caaactacgg
     1921 caacagcacc agagactatt acggcactag ctaccctatg gatatttcag agtttcacac
     1981 ttacaaaatg acttgggata agaatgccat cgccatgtac gtagacgact ttaagtacca
     2041 agaaatcgcg atcgccgaag caggcgacat ggacaccttc cacaagaaat ttttctttat
     2101 cttgaacgtc gcggtagcag gcacctggcc tggtttcgaa gtggacgaca cacagttccc
     2161 caccgaaatg ctcgtggact acataagagt gtacaagaac actagtaact aaatgtattt
     2221 aatatcaatt tactatacta tcatactatg aaaaattcta tgaaaattct ttgccttatc
     2281 ctatttatcg ccattttttc ctttgcgaca ctttctaaag aaacccgcac aaactttgtc
     2341 agcagctgta ttgatggagg ctcgtcaagt gttttttgcg aatgtgtttt caaaaagatg
     2401 gaacaaaaat atacccaatc gcaaatcgac gccattgaac ttaaaatgca aagcggacaa
     2461 agcgacctag gctattccaa ttttatcaaa agtacttcta aagaatgcaa tgcaaaaacc
     2521 aaatcgggca cgtccctcgg ggcaatggcc gcaaacgaaa ataaaagtaa tcaaaaaacg
     2581 caatccactc tttcaagtgg agattttgag gcaatggaaa atatggacat tcaagctgcc
     2641 tttgctcaag gaatgatggc cgccatgata gaatcgcccc aattcaaaaa catgttcata
     2701 gcacaatgcg atacagagtt gattccatac tttggtaaga aacaggcaaa aagcacttgt
     2761 gaatgcagtt acgagaaaat actttctagt ggttacatac aaaaaatctt gaattcaatc
     2821 aatgaggaag ggaaattaga ggactctttg accgttgaac tttttatgcc atgcattcct
     2881 aaaacgtata cgccacaaat ggagcagttc ttaatgagct cttgccaaac gaaggcaacg
     2941 aaaaatattt gcaattgcat tgtaaaagac atcaagaaaa gttttacgct agaacaattg
     3001 ctgcgtaaaa cgttgactga tccgacattt attgagagtt atgcgacagg tgccgcgatg
     3061 cgttgcaaaa ataattaagt tcaaaatgtg tatgtaaaat taaaggagaa taaagatgtg
     3121 tgtctggaaa aatattttga ttgtaacggc tgtggcggct tctcttgcca cgtctgcatc
     3181 gatttcaatt gaccccacgg caacggcgca aaagattgtt ggctttggcg gtggcgtggt
     3241 gtattatcaa ggttggtttg tcgccttgtc tccagaatct aaagaagctc tttatgatac
     3301 ggcgtttaca ggcctaaatc tttcgctctt gcgtttgggc aactggatgc aaacagatac
     3361 aaatgatatt tctgcagatg tagagattgt gaaagccgcc aaagatcgtc ttggagatca
     3421 cttgaaaatt caaatgtcga gttggacagc tccagcaaac ttaaaaccta gcggcgatga
     3481 gaacggttca ccaacacaat caggtaacga caagtatcca cggaagagtg ctacattaaa
     3541 tacatcgact tcggatccat acggcaaata cgcctataca gactttgcga actggtggaa
     3601 aaaatcgtat caagcttaca ccgacgcagg cattaacata gactatatca gtttgcaaaa
     3661 tgaacccgat atgtttgccg cctatgcaga aacgcttttt gatgagacag aaaatgacag
     3721 cacagcgggt tacgctcaag cactaaatgc cgtttatgat gccatgaaaa ctctccccaa
     3781 tcctccaaaa attcttgggc cagaacctct tggcatcggt tataacaatt ttcaaaaata
     3841 cgccaatgcc ttagacgcat ctaaactcga tggctacgct tatcacttgt atcatgctgg
     3901 tgatggcaat gataattcgc tgaacaatta tttgaatcca gaaaattata gaaaacctat
     3961 gtcacagatt gcgaattcat tttattcgga atccaaacca attatcatga cagaattctg
     4021 cactatggaa gagaatggta aagaaaatta tatggtgggt ctcgcacaca ttatgcaagt
     4081 tggctttacc gcagggaaac tcggcggtta cattgcttgg gaactgatgt ggggcgatgg
     4141 tgttggacag ctcattggcg tttgtactaa aggctggggc agctgcactg aagatgctat
     4201 aaccatttct ccagaatacc acgctatgcg tcactactcc aaatttgtga atcctggatg
     4261 gagtgtagtg aatgcaaccg ttacagacgc tacaaacctc tatgtagtcg ccttccgttc
     4321 tgcagatgaa gattcactca cggttgtcgc tgtgaacagc aattcggcgg caacatcatt
     4381 gacatcgcta gatgtcaaaa actacaaggc aatagacgct gtgcaatcgg tggaaaatgg
     4441 agagaagagc aagaaaatcg cgacagcaaa ttcttacacg cttcctgcaa aatcgataac
     4501 gacttttgtt tttagcggcg aaagtaatcg agtaattcct gccgaaattc atttgaatcc
     4561 aaatgcaacg agtccgattt ctcaagtttt tgatttgcaa gggaatttgc tttggacagg
     4621 aaaactcacg aacgcagaat tgacagacgg tgttttgcac ttgcaaaatt taaacgctgg
     4681 tctttacctc gttcgccatg gttcaaagac aatcactgct gtaaagaagt aaaaagagat
     4741 aaatcacatt cgctattgca aaatttgcaa ataatacaat acaatcttaa atgaaattca
     4801 atgggcttga tgctatataa ataaatgcat aaggcccatt tctacttgag aagttattta
     4861 ctccatcaaa tattcaaata gtaagtaggg cgtatgccta gttttgcttc gtcttcggcg
     4921 acgctttctt ttggtgtgtt acgccctaaa atcaaagcag aatcgattcc tgcagcacag
     4981 gcgcccaata cgtctgttcc gagagaatct ccaatcataa tggcttttga agatttagga
     5041 atttttttca acgtttcaga aaatatggtt ggaaaaggtt ttcctaaata aatcacttca
     5101 gcattcgttt gctgaatcaa ataatatgca aaagctcccg agacgggctt tcgagagcca
     5161 tcgatacttg gagcccaagg gtctggattt aagacaatca atttggcatt cggtcttttt
     5221 aatatttcga ttgctttttc tcgtaactcc acttcgcgta ctacatttga aaggatcacg
     5281 actggatctt gtgggttttc agaaaaagta atccccgccg cttctaaaaa agcaacgcct
     5341 gtttcgcgtc ctaaataaaa tgcttcttga atattttgct tttgattttc aagcgttaaa
     5401 agatcgcccg aagtcacgac ttcttcatgt aaaaagtcaa accccataag ccataaatct
     5461 tttaataatt tcactactgg ctgcgaggcc gcattcgtca gcaagcggat tgttttcccc
     5521 ttctgcctta aatagcgaac catttctaaa gcacctggat atacgtaatg attttgatta
     5581 tagagagtgc cgaaagcatc taaaaaaaac aagtcgtatt taaatgctaa ttgttctaat
     5641 gtatttattt tcgtttctat cgtttgaaaa gaaacattta atcgttcaat atatttttta
     5701 tgaatttcta gttctaatgg ttccataaac tatcgtctta aaacacccca tgactgcatt
     5761 cgattttctt tatctttcgt ttgataaaca gcatggccat tgtaagaaat acggacatcc
     5821 acatcgacta aataaacacc tgtttgcaca aaacgaccgt catcacttaa aaagttccaa
     5881 cggaagaaaa ctcgttttcc tcgattttct aaccgtgaat cagaaccctt aaacgaaact
     5941 tcatcactcg caacataagt cccaagacta gtgaaaagac acattttaat gacaatctta
     6001 attttcgata tatcgatttt ttcgtctttt cctatttcaa gtgaagggta acgcaaacgg
     6061 agttcttctc gaatcaccgt ttcaaattct ggccctccta catctagcgc cacgccaaca
     6121 gcagtatcat taggatagag agactcgaaa ggtaaaaaat caagctcaaa aacgcgccca
     6181 ttttttttct tactattttc aaaataagtc gctttattaa ctgtaaatat ttctatagga
     6241 taagagccct ttaacgccac aaaaggagtc gattctggga tttgatttct cgaggaatcc
     6301 ataatgcagc ttgctttaat ccgaatagaa tcttttggtg tcatggtggt ttcagtaaaa
     6361 agttctttat ctaaaataaa gaaagcttcg cgatgtaaaa cattccaata gtacgaagaa
     6421 tattgccaaa gcgatacgtc atcaccactg gctgttttat actctataat caaaccacaa
     6481 ttttgattat caaaagaagg ctcagagaat acaatcttta aggtgtcatc cccacgtcca
     6541 cggtaactct taaaaaaagt ttcctgtatc actggagcca tttgatcttt catgctaatg
     6601 aaaatagcat ctccgttact ctgatacaaa atagctgagc cataatctcc ataagagaaa
     6661 tcatttaagg aagttaaata cggctgcatt ggaacaggag atggaaaatc aaaataaaca
     6721 agacggttat aggaggaatc tattttcaaa gaagagggag ttatagtaaa agaagttctt
     6781 tgaga
//
LOCUS       DAOOBB010000100         6744 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_13403_length_6744_cov_5.000299, whole genome shotgun sequence.
ACCESSION   DAOOBB010000100 DAOOBB010000000
VERSION     DAOOBB010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 6744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6744
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13403_length_6744_cov_5.000299"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PLT31_05685"
     CDS             <1..305
                     /locus_tag="PLT31_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94666.1"
                     /translation="SASSGISNVFTYNVYASVFYKAFSLNYSVTAYTPVNDANESNND
                     YYVDAGFSLPLVLTEYITASYRINKNWEVRAGAINVFGNFPGWHWAATSGITYYFK"
     gene            478..5661
                     /gene="uvrA"
                     /locus_tag="PLT31_05690"
     CDS             478..5661
                     /gene="uvrA"
                     /locus_tag="PLT31_05690"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPW94667.1"
                     /translation="MTRSVVVRDAHEHNLKHVHVTIPRDSIVVITGVSGSGKSSLAFD
                     TIFQEGQRRFLDSLSAYARQFIGSMKRPEVESVRGISPTISIDQKTVNRNPRSTVGTV
                     VEIMDYYRLLFARLGVPHCPQCGKKIEAQSVDQIVDQLYSEDEGSSLLVMAPIVQERK
                     GEYRKEIEDLITNGFIRARVDGVIYRLEDIPLLVRYEKHTIEVVIDRLDLSKKNISRV
                     REAIEGALRLTEGKLVSFLFNESKYLLQGTELACVRCGISIPELEPRLFSFNDVQGQC
                     PSCKGLGRNYQFTKELIVPNPSLSIKQGALAPQKKDGDIIFSSFGWSDLKAIAKEMKF
                     SLDTPWESLSINQQEAILYGPPSKKVSGVVPTMQALWDKWHIHLFKKYMHESICHTCN
                     GSRLNPVANAVRFHGYGIQDLSSWSVNQSVKFFKEQKYTPREEHIGKEIFKEINNRLS
                     FLEAVGLGYLNISRSSATLSGGEAQRIRLAGAVGAGLQGVLYVLDEPSIGLHPRDNEK
                     LLDILEHLRAQGNSLIVVEHDEETMRRADCVIDIGPGAGVKGGKIIAMGTAEELEKNK
                     KSLTGLYLSGQKKIEIPQKRKRIDSKTPFLTIYGARENNLKNIDVSIPLDGAFTVVTG
                     VSGSGKSSLVNHILRRELARYFHHAEEEPGLFDRMEGLEHIDKVIEIDQTPIGRTPRS
                     NPVTYTKIFDSIRDLFASLPESKVRGYTKSRFSFNVPGGRCEMCEGAGVKTVDMQIMP
                     SVEIPCEFCDGKRFNEATLEVYYKGLNIYDVLEMSVDEACVFFESIPKIVEPLKLLQE
                     IGLGYLKLGQPSTTLSGGEAQRIKISAELRRPGTGKTLYLLDEPTTGLHFEDIHRLLD
                     CLNRLRSLGNSLVVIEHNLDVIKCADWIIDLGPEAGDAGGYLVATGTPEEIAKNKKSM
                     TGRFLAPVLKGETWTQTKKSSKSVFEDSFSLDIQIHGARKHNLKNFDVTIPRHQLTVI
                     TGVSGSGKSSLAFHTLFAEGQRRFVETLSAYARRFLGRLDRGHIDSITGLAPAIAIDQ
                     GSSSRSPRSTVATLTEIYDYFRIFWSRLGIPHCIHCGKPLQSFSVAEVSHFALKHFKN
                     QMVHVLLSDHHSTHKLEAMLEKGYKKKTSGNKEYIQIDRVIVNEKNKKRLVEAIEIAY
                     KENQGKVILEGESNQRHLFSLLPGCNSCDWYFEQELNPKFFSFNTHWGACPRCLGLGI
                     EDSQKICPECHGMRLKKEYLNVRIAEKNIMEVSSMSISKARDWFAERIFTQDNAKIAD
                     PLLKEILGRLDFLKNVGLGYLGLFRTGDTLSGGEAQRIRLSSQIGSGLEGVLYVLDEP
                     TVGLHQNDTQKLLSTLYQLRDLGNTLVVVEHDLEMMRAADHIIDMGPGAGEYGGEIVA
                     EGSPKKLSQKNALTLYPRSETVRYLTSKTPLHNQNTPEVVSDETPFYEFNHLSKNNLK
                     NIDVRIPKGKISVVSGVSGSGKSTLVMDCILPELKKQFGPRGRRRSSVDVVVVDQSPL
                     SGTPRSTPASYTGALDPIRRLFAKMEQSKMKGFDLGRFSYNVARGRCPICEGRGYISV
                     AMHFLSDVWELCEACEGKRYNQETLSVLFKGKNIADVLDLRIDEACEFFKDQKKIVSL
                     LKPLSEVGLSYLRLGQSVTTLSGGEAQRMKLAAELSVNEEKETVYLFDEPTTGLHLKD
                     IQVLWDMFRRLSDRGHTVVIIEHHPEIIRLADWNIELGPEGGDLGGFLLFQGFFKHKA
                     T"
     gene            5707..>6744
                     /locus_tag="PLT31_05695"
     CDS             5707..>6744
                     /locus_tag="PLT31_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94668.1"
                     /translation="MNINVLITCLGLLVGISVHATTDKHFIIPKEQFREGVLIKNDKS
                     CLAQEGKSLLSSTPKFPLESNTLYWRHKDNPREYNWFFGTTRIDCFGKLKAFNLKKNN
                     LTAFEATYMRFYGTARAKSYQDEADLYFDSEYLYSQRVSTLYYMSNGKLKKLESEPLP
                     GVLKVETTLSDYSFIVDGYNYGPAKKIFPLSPGLFYGTLVANGYLPYVEGALIVSGKE
                     TILKPTWVGIFKNHKKIPTSITEEKIKSTASLEEVEALYDVFIEELHQLPGEVFVDRF
                     DSVYPKPKQFFFRLDDSEIYNKYLELFKIQREQAKVLWNESKLTVVKASYEALRSKLD
                     SLEQLPLRIMLF"
BASE COUNT         1988 a         1218 c         1461 g         2077 t
ORIGIN      
        1 tctcggcatc ttcaggaata tcgaacgtgt ttacatacaa cgtctatgcc agcgtcttct
       61 acaaagcatt ctccctgaac tattccgtta ccgcctatac tccagtaaat gatgcgaacg
      121 agtccaataa tgactattac gtagatgccg gattctcctt gcccctcgta ctcactgagt
      181 acatcacggc tagttaccgc attaacaaaa attgggaagt ccgtgccggt gccatcaatg
      241 ttttcggaaa tttccctggc tggcactggg cagctaccag tggcatcacc tattacttca
      301 agtgatttgg ctctgcaggg ctaaaacgta cggctgcttc cgaaacctaa aggtctttct
      361 attgactatt gttcatttgt tcactatctt ttactaaaaa ataggcgaaa aagcttttga
      421 atcgtttttt ttatcccttt tttgtctatt cctcttcgtt caaggaagta tttgtatatg
      481 actcgttcgg tggtagtgcg tgatgctcat gaacataatc tgaaacatgt tcatgtgact
      541 attccaagag actccattgt ggtaatcaca ggtgtttctg gttcagggaa atcgagcctt
      601 gcttttgaca ctatttttca agaggggcag cgtcgctttt tagattctct ttcggcctac
      661 gcgcgtcaat ttattggtag catgaaacgc ccagaggtcg aaagtgtacg cggtatttct
      721 cctactatat ctatcgatca aaaaacagtc aacagaaatc ctcgttctac tgtcggtacc
      781 gttgttgaaa tcatggatta ttataggttg ctttttgcgc gattaggcgt gcctcattgc
      841 cctcaatgtg gaaagaaaat tgaggctcaa tctgtagatc aaattgtgga ccaactttat
      901 tctgaagatg agggcagttc tcttttagtg atggcgccga ttgtccaaga acgtaagggt
      961 gagtatcgta aagaaattga agatctaata accaatggtt ttattcgtgc gcgagtcgat
     1021 ggggttattt atcgtttaga agatattcct ttattagtgc gttatgaaaa gcatacgatc
     1081 gaagtcgtta ttgatcggtt agatttatct aaaaagaata tttctcgtgt gcgtgaggcg
     1141 attgaagggg cgcttcgttt aacagaaggg aagcttgttt catttttatt taatgaatct
     1201 aaatatcttc ttcaaggtac ggaactagcc tgtgttcgtt gtggtatttc aatccctgaa
     1261 cttgagcctc gattattttc ttttaatgat gttcaagggc aatgcccttc ctgtaaaggc
     1321 ttagggcgaa attatcaatt tacaaaagaa ctgattgttc caaacccttc attatctata
     1381 aagcaagggg cattagcgcc tcaaaaaaaa gatggtgata tcattttttc ttcgtttggt
     1441 tggtcagact taaaagcaat tgccaaagag atgaagttct ctcttgatac tccatgggag
     1501 tctctttcta taaaccaaca agaggccatt ctttacggac ccccatccaa aaaagtttct
     1561 ggtgtggtgc caacaatgca agcgctttgg gataaatggc atattcatct ttttaaaaaa
     1621 tacatgcatg aaagcatttg ccatacttgt aacgggtcgc gcttaaatcc tgtagccaat
     1681 gcggttcgtt ttcatggtta tggcattcaa gatttgagta gctggtctgt gaatcaatcg
     1741 gtaaagtttt ttaaggaaca aaaatacact cctcgtgaag aacatattgg aaaagaaatt
     1801 ttcaaagaaa ttaataatag gctttctttt ctagaagcag taggattagg ctatcttaat
     1861 atttctagaa gttctgccac tctttcgggt ggtgaagccc aacgcattcg cttggcaggc
     1921 gctgtgggag cgggtttgca aggcgtgttg tatgttttag atgagcctag tattggttta
     1981 catccaagag acaatgaaaa actattagat attttagaac accttcgcgc gcaagggaac
     2041 agcttaattg tcgtagaaca tgatgaagaa accatgcgac gggctgattg tgtgattgat
     2101 attgggcctg gggctggtgt caaaggcggt aaaatcattg cgatgggaac cgcagaagaa
     2161 ttagaaaaga ataaaaaatc gcttacgggg ctttatctta gtgggcaaaa gaaaatcgaa
     2221 atccctcaaa aaagaaaacg aatcgattcc aaaacgcctt ttttaaccat ctacggtgcg
     2281 agagaaaaca acttaaaaaa tatcgatgtt tcgatcccat tagatggcgc gtttactgta
     2341 gtgacagggg tttcaggctc tggaaaaagc tctctagtga atcacatttt gcgccgtgaa
     2401 ctcgctcgct attttcacca cgccgaagaa gaacctggtt tatttgaccg catggaaggc
     2461 ttagagcata ttgataaagt catagaaata gaccaaacac ctattggaag aacacctcgt
     2521 tccaatcctg tcacgtacac gaaaattttt gattcgattc gagatctatt tgcttcttta
     2581 ccagaaagta aagtacgtgg gtatactaaa agccgtttta gttttaatgt ccctggaggg
     2641 cgttgtgaga tgtgtgaagg ggctggcgtt aaaacggtcg atatgcagat tatgccaagc
     2701 gtagaaatcc cctgtgaatt ttgcgatggg aaacgtttta atgaagctac acttgaggtc
     2761 tattataaag gcctgaatat ctatgacgtt ttagaaatga gtgtcgacga ggcttgtgtt
     2821 ttctttgaat cgattccaaa gattgtagag ccattaaaac tcttacaaga aattgggctt
     2881 ggctatttaa aattagggca accttctacg actctttctg gaggagaagc gcagcgtatt
     2941 aaaataagtg cagagcttcg tcgtccaggt acaggaaaaa cattgtatct actagatgaa
     3001 cctaccacag ggcttcattt tgaagatatc catcgcttac tagattgctt aaatcgattg
     3061 cgtagtttag ggaatagtct tgtggttatt gaacataatc tagacgtgat taaatgtgcg
     3121 gattggatta ttgatttagg gccagaagcc ggagacgctg gtgggtattt agtagctacg
     3181 ggtactccag aagaaatagc gaagaataaa aaatcgatga caggtcgctt tctagctcca
     3241 gttctcaaag gagaaacatg gactcaaact aaaaagagtt ctaaatctgt ttttgaggat
     3301 tcattttctc tagatattca aatccatggc gctagaaaac acaatctcaa aaattttgat
     3361 gtgacaatcc caaggcacca acttacagtg attacaggag tctcgggttc tggtaaatcg
     3421 agccttgctt ttcatacgct ttttgcagaa ggccaacgcc gttttgtaga aacattaagc
     3481 gcttatgccc gccgcttttt agggcgacta gatcgcgggc atattgattc tatcacaggc
     3541 cttgctccag cgattgcgat agatcaaggg agttcgagcc gtagcccgag aagtacagtc
     3601 gcgacgctca ctgaaattta tgattacttt agaatttttt ggtctagatt aggaattcct
     3661 cattgtattc attgtggaaa gcctcttcaa tcttttagtg ttgcagaagt ctctcatttt
     3721 gcacttaaac attttaagaa tcaaatggtg catgtcttat tgagtgatca tcattctact
     3781 cataaattgg aggccatgct tgagaaaggc tataagaaga agacttcagg gaataaagaa
     3841 tatatccaga ttgatcgcgt gatcgtcaat gaaaaaaata aaaaacgatt agtcgaagcg
     3901 attgaaatcg cttataaaga aaatcaaggg aaagtgattc tcgaagggga gtcgaatcaa
     3961 cggcatttat tctccttgtt gcctggttgt aattcttgcg actggtattt tgagcaagaa
     4021 cttaatccta aatttttcag ctttaatacc cattggggcg cttgtcctcg ttgtttgggg
     4081 ctaggcatag aagattcgca gaagatttgc ccagaatgcc atggcatgcg actaaagaaa
     4141 gagtatttga atgtacgcat cgccgaaaaa aatatcatgg aagtctcttc gatgagtatt
     4201 tcaaaggcaa gagattggtt tgcggagcgc atttttactc aagataacgc taagattgca
     4261 gacccgttat taaaagaaat tctcggccga ttagactttc ttaaaaatgt tgggctcggt
     4321 tacttaggcc tatttagaac aggggatacc ttatcaggag gcgaggcgca acggattcgc
     4381 ttgtcgagtc aaattggaag tggtttagag ggcgttcttt acgtgttaga tgagcctacc
     4441 gttggcttac accaaaatga tactcaaaaa cttctttcca cactgtatca acttagagat
     4501 ttaggcaaca ccttagtcgt tgtagaacat gatttagaaa tgatgcgggc agcagatcac
     4561 attattgata tgggccctgg cgctggggaa tatggaggag aaattgttgc cgaaggttct
     4621 cctaaaaaat tatctcagaa aaatgcgctg actctttatc caagaagtga aacggttcga
     4681 tatcttacct caaaaacgcc tcttcataat caaaacacgc cagaggttgt ttcagacgaa
     4741 actccttttt atgagtttaa tcacctttcc aagaataatc taaaaaacat agatgttcgt
     4801 attcctaaag ggaaaatttc ggttgtttca ggagtttctg gttctggcaa aagtacttta
     4861 gtgatggatt gtattttacc tgaattgaaa aaacaatttg ggcctagagg gcgtcggcga
     4921 agctctgtgg acgtcgtcgt tgtcgatcaa tctcctttat caggaactcc gagatctact
     4981 cctgcaagtt atacgggggc tttagatcca attcgtcggc tttttgccaa aatggaacag
     5041 tctaaaatga agggctttga tcttggccgc tttagttata atgtggcaag aggtcgctgt
     5101 cctatttgtg aagggcgtgg gtatatctcc gttgcgatgc actttttatc cgatgtgtgg
     5161 gaactttgtg aggcttgtga ggggaagcgt tataatcaag agactctatc cgttcttttt
     5221 aagggaaaaa acattgcaga tgtcctagat cttcgtattg atgaagcatg tgagtttttc
     5281 aaagatcaga aaaaaatagt ctcccttcta aaaccacttt ctgaagttgg tctttcttat
     5341 ttaagactgg ggcagtcggt aaccactctt tcaggaggcg aagctcagcg tatgaaatta
     5401 gccgctgaac tttctgtaaa cgaagaaaaa gaaaccgtat atttatttga tgaacctacc
     5461 acaggacttc atttaaaaga tattcaggtt ctttgggata tgtttagacg cctttctgac
     5521 cgtggacaca ccgtagtgat tattgaacat catccagaaa tcattcgttt agccgattgg
     5581 aacatcgaat tggggccaga aggcggggat ttaggtggtt ttttactatt tcaaggtttt
     5641 tttaaacaca aagcgactta aagaaaagta ttttttcttt aatatgattg gtattttgga
     5701 ggctttatga acataaacgt gctaatcaca tgtttaggtc ttttggtggg tatttctgta
     5761 catgcaacca cagataagca ttttattatt cctaaagagc agtttcgtga aggagtgctc
     5821 ataaagaatg ataaatcttg cttagcacaa gagggaaaaa gccttctttc atcaactccc
     5881 aaattccctc ttgaaagcaa tacgctctat tggcgtcata aggacaatcc tcgtgagtac
     5941 aattggtttt ttggaaccac tcgcattgat tgctttggaa aattaaaagc ttttaatttg
     6001 aaaaaaaata atctgactgc ttttgaagca acttatatgc gcttttatgg aacagcgaga
     6061 gctaagtcat atcaagatga agccgatctc tattttgatt cagagtactt atattcacaa
     6121 cgtgtttcta ctctatatta tatgagtaat ggaaagctta aaaaattaga gtcagaacca
     6181 ttaccaggtg tactcaaggt agaaacaact ctttccgatt attcctttat tgttgatggt
     6241 tataattatg ggcctgccaa aaaaatattt cctttgtctc caggtttatt ttatggtaca
     6301 ctagtggcta atggttatct tccttatgta gaaggagccc taattgtatc aggaaaagaa
     6361 actatcttaa aaccaacatg ggttggtatt tttaagaatc ataaaaaaat tccaacttca
     6421 ataacagaag aaaaaataaa gagtacagct tctttagaag aagttgaagc tttatatgac
     6481 gtatttatag aagagcttca tcaacttcca ggagaggtct ttgttgatcg atttgattct
     6541 gtttatccta aaccaaagca gttctttttc cgattggatg attcagagat ttataataag
     6601 tacttggaac ttttcaaaat acagagagag caagcgaagg tattatggaa tgagtccaag
     6661 ctaactgttg taaaagcttc ttatgaagca ttgcgttcta aactagatag tttagaacag
     6721 ttgcctttac gtatcatgtt attt
//
LOCUS       DAOOBB010000101         6703 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_13507_length_6703_cov_4.177647, whole genome shotgun sequence.
ACCESSION   DAOOBB010000101 DAOOBB010000000
VERSION     DAOOBB010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 6703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6703
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13507_length_6703_cov_4.177647"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="PLT31_05700"
     CDS             complement(<1..456)
                     /locus_tag="PLT31_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HPW94669.1"
                     /translation="MGFKKIGVYFLCFTGVLLAYTPPSGAVSKLNSYRGYSDLTKAAG
                     SMDIDTYTYNLTTWQINHGGFYKAYASKYVNPWNGSDAKSDIISKDGTPLGTIDNNAT
                     IQEMRLLAVRYKSTTNSSYKTAFKSSFSKAIGFLVAAQRSKGAWPQMHPK"
     gene            complement(1219..1293)
                     /locus_tag="PLT31_05705"
     tRNA            complement(1219..1293)
                     /locus_tag="PLT31_05705"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1256..1258),aa:Asp,seq:gtc)
     gene            complement(1305..1379)
                     /locus_tag="PLT31_05710"
     tRNA            complement(1305..1379)
                     /locus_tag="PLT31_05710"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1342..1344),aa:Val,seq:tac)
     gene            complement(1656..3923)
                     /locus_tag="PLT31_05715"
     CDS             complement(1656..3923)
                     /locus_tag="PLT31_05715"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPW94670.1"
                     /translation="MKINKNKISIVVGWIFISLLAIFLFPDKDLAQKAETPHLGQMSP
                     RTIIAPISFDVPKTAQELEIERQKAADKVNAVFEFNHDESKRSLDDLKQFLSKVGAYG
                     SLQYAIAESNDPDSVQMFTQQASRMFDVLKQRLSTTAVQKLSQDSKARDSLYSAFNRM
                     IQKGISNTLIANTETSVQLFRDNYNIQDVQFIPYTNTTISFIKDNVESNIDASQIQPR
                     ERRIDETFAELQMSFSKSQGLQSAFYEALYVFTLPNVFYLEKETEVRKKTAQQQVHLS
                     KGMIPRGMEIVSQGSIITKDILEKIDALQNALQKEEGSKTLTATYGQGLLLLAVVSGF
                     FLFFMLFASDSVKKTNHIWSIIASAAIQLLLFWLFQRISGVLNKPDSILSDSIDLIWI
                     YPFSIAPIIATVLYERRIGLIFSLFSSVMMSFLSGYDLAIMISSFFISWASIHLLARM
                     RYRVQFAWGILSGVSMLALMLVIILLLRNRLDFEIFFSTFIAGTINLIFCTAIASVLL
                     IHLAEKVFGITTVLTLMEMADFNRPALKRLSELAPGSFHHSIQVANLAEKVAEYIGAD
                     SLLVRVMALYHDLGKTMRPEFFTENQKQGINPHNSLDPFQSVKIITGHVEQGISLAKE
                     YNIPELVAAAIPEHHGTTVIQYFYHTAKELYPDRTLKEEDFRYKGPKPQSKESAILML
                     ADSIEATSRALSDSSSDALAEMIRKSIDLRLKEGQFSETDLSVKDLFKLEKAFLQSLD
                     GTYHTRVKYPSQNKT"
     gene            complement(3920..4885)
                     /locus_tag="PLT31_05720"
     CDS             complement(3920..4885)
                     /locus_tag="PLT31_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPW94671.1"
                     /translation="MSEPIRYPISDDLKRLMAGENETVFRILESRFCVSIQTRLPGLF
                     IEAKEERSNIQATLTLLDQIKLAAQNGTSITGDWMRRLLEPQYEVTSNELESIPSEPI
                     LRNRYGFAVVPRTKNQYDLIKAVEKNDIIFAKGPAGTGKTFLAVALAVASLERHEAER
                     ICLVRPAVEAGESLGYLPGDLKEKIAPYLRPIHDSLAELLAPEKLKRYQETGAIEVAP
                     LAYMRGRTLKRAFIILDEAQNTTVAQMKMFLTRLGTHSKAIITGDASQVDLGKGTVSG
                     LNHAIDLLQNIHGIAHVEFNGTDVLRHRLVKDIIAAYEKNAGQMK"
     gene            complement(4882..5175)
                     /locus_tag="PLT31_05725"
     CDS             complement(4882..5175)
                     /locus_tag="PLT31_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014544912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94672.1"
                     /translation="MSLLKNFKLLLIAGTVVLFAACAGSSGGSGSSDDEVYYEDDGTG
                     TKKAVKKEVITEDKLKDTEKEATALTEENHKLRREIFDAKHQLGIPVEQQTGE"
     gene            5261..6658
                     /locus_tag="PLT31_05730"
     CDS             5261..6658
                     /locus_tag="PLT31_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HPW94673.1"
                     /translation="MEAIKQLLQDYSEVFISTFSEKTAGIYQKRRRELLSKLDSFCVF
                     SGVAVEPGTEEVFASTWTRFIQDPSFLFLTGINQAGCFLLLDPLAKEESMREVLFIPL
                     KDKDKEFWVGKRIGYSDDLSDLKTLTGFQSIQPSSSLWNTIERLSLRKETPSYAYAFF
                     FPDYQLDHNARFALEMGTFLEKKQKTLRSVSEIHLELRMVLDRERISLVEKAQEASAE
                     AFKSILSRLPLFKNERALGLALDYELQSRGDGDLAFPTIVASGKNACCLHYSKKDESL
                     QEKDLVLLDFGIRIGTQHSDISRTIPVSGQFDPLQKLLYNIVLEAQLFHEKQVRPGVT
                     LRELDQNVWDFIEKSLKNSFTDKGGTFELLYNKRPHGVSHFIGEQVHEGAPKSRSLDV
                     VLKPGMMISNEPGLYGRFQMTLDGVHYDRLIGIRIEDDLLITENGCINMSIDIPKQVE
                     ELERLIANGSNFFNV"
BASE COUNT         2095 a         1201 c         1261 g         2146 t
ORIGIN      
        1 tttaggatgc atctgaggcc atgcgccttt agaacgttga gcggctacta aaaaacctat
       61 agctttgcta aaggagctct taaaagccgt tttataactg ctattcgttg tgcttttata
      121 acgtacagcc aaaagcctca tttcttgaat ggtggcgttg ttgtcaatcg ttccaagagg
      181 cgtaccgtcc ttgctgataa tatcggattt tgcatctgag ccattccaag ggtttacgta
      241 tttagaggcg tatgctttat aaaatccacc gtgattaatt tgccaagtgg ttagattata
      301 agtataggta tctatatcca tagatcctgc cgctttggtg aggtcagaat accctctata
      361 actgtttaat tttgatactg cgcctgatgg aggggtataa gccaaaagaa cccctgtgaa
      421 acataaaaaa tagaccccaa tctttttgaa acccataaat actccgtttt aattttgtct
      481 caaaataaaa ataccctttt aatagaaatt atatatgaaa agacttataa agggttgttc
      541 ttagacaacg atattatgga caaaatggaa acaactctct gttgttctgc gtgatggctt
      601 tgtcgaaatt aaacttgatt gtagaggctt ttttcgtaac gaacccccaa agtgaaaaaa
      661 tgaataaaaa gccagaattt atgcggaaaa atcactattt agggttttta ttgacaacgc
      721 atttcgctaa aaaaatacac gtaatgctta aacagaatat taagtgtctc agaaactcca
      781 aaaaatgtgc ttttgtgaag aaacttagtt gtttataaaa acgggacttg cggaaagata
      841 gtcacagacg caaaaatttt ttataaaaaa acttagggca tcatctatct cttcacattt
      901 tccactatcc ttaaaaagga ttacacattt gagtatatcc tcatcaaaac catccttttt
      961 cagtacctat aatctgtgta ttcaaggaaa tcaagtgata ttgtcttatt ttggaatgtt
     1021 ttgaaaaact ggataatcaa gctctattta agaaaacatg ttttttttgg acagtagtgt
     1081 aatggtgtag gaatcaaaaa aaaatcttat aaaaaagctt cttataaaat tcttcagaga
     1141 gtacgagctc actttctaat gatttttagc aaaaaagtgt tatataaaaa aagaaccctc
     1201 taaaaagagg gttctttacg ggatagacga ggcttgaact cgcaacctcc ggcgtgacag
     1261 gccggtgctc taaccaaatt gagctaccac cccaaaaatt ggtatgggcg ataacggatt
     1321 cgaaccgctg acccgctgct tgtaaggcag ctgctctgaa ccagctgagc taatcgccct
     1381 tagtctttga atctttgcta ccccatagaa ttccgatagg aattactaca agataagcca
     1441 aaatcaaaat aattggtgcg actgtcaggg agaggggatt atttgctggc ccttgagcca
     1501 aacagaaaaa cccgatcatt agcaatgcaa ctccgagggt tatcaataga aaagtctttt
     1561 tgtttttcat tactgtccta ccgacgttac caccaatatt acaggcccaa atatattaag
     1621 tgaaaacgtc tttgtcaagt tattttagtt tttttttaag ttttattttg agacgggtat
     1681 tttactcttg tatgatatgt tccgtctagg ctctgtaaaa atgctttttc caatttaaat
     1741 aaatctttta cactaagatc agtttcgcta aattgacctt cttttaatct taaatcaatt
     1801 gattttctaa tcatttcagc aagagcatca gaagaagaat ccgataaagc tcggcttgta
     1861 gcttcaatac tatcagctaa cattaaaata gctgattctt tgctttgagg tttcggtcct
     1921 ttatagcgga aatcttcttc ttttagtgtt ctatcaggat acaactcttt agccgtatga
     1981 taaaaatatt gaatcacggt tgttccatga tgctcaggaa tcgctgcggc cactaattca
     2041 gggatattat attcttttgc aagagaaatc ccttgttcca catgccctgt aataatcttt
     2101 actgactgaa atggatctag tgaattatgt ggattaatcc cttgtttctg attctcagta
     2161 aaaaattcag gcctcattgt tttcccgaga tcatgatata aagccatgac tcgaacaagt
     2221 aaggagtctg ctccaatata ttctgctact ttttctgcga gattagcgac ttgaatgcta
     2281 tgatgaaaag aacctggagc tagttcagaa agccttttta acgcaggacg attaaaatca
     2341 gccatttcca ttaatgttaa aacagtggta ataccaaaaa ctttttctgc taagtgaatg
     2401 agtaatacag aggcaattgc agtacagaaa attaagttaa tcgttcctgc aataaaggtg
     2461 gaaaagaaaa tttcaaaatc gagtcggtta cgaagtaaaa gaataatcac aagcattaat
     2521 gcgagcattg atacgccaga taaaatgccc catgcaaatt gaacgcggta cctcatgcgt
     2581 gcaagcaaat gaatcgaggc ccaagaaata aaaaatgaac tgatcataat cgcaagatca
     2641 taaccagata agaagctcat catcacagaa gaaaataaag aaaaaattaa tcctattcgt
     2701 cgttcgtata agacggttgc tataataggg gctatactga aaggataaat ccagattaaa
     2761 tcgatcgaat cagaaagtat agaatctggt ttgtttagga ccccagaaat tcgttgaaat
     2821 agccagaaga gtaatagttg tattgccgct gaagcaatga tagaccaaat atgatttgtc
     2881 tttttgacgg aatcactcgc aaatagcata aagaataaaa agaagcctga gacgacagcc
     2941 aaaagaagta acccttgacc gtaggttgct gtaagcgtct tagacccttc ttctttttga
     3001 agtgcatttt gcaaggcatc tattttttca agaatatctt ttgtaataat ggagccttga
     3061 gatacaattt ccattcctct aggaatcatt cctttagata aatgaacttg ttgctgtgct
     3121 gtttttttgc gaacctcggt ttctttttct aaataaaaaa cgttaggtag agtaaataca
     3181 tagagcgctt cataaaaagc actttgtaat ccttggcttt tagaaaaaga catttgtaat
     3241 tcagcaaaag tttcatcaat gcgcctttca cgaggctgaa tctggcttgc gtctatatta
     3301 ctctccacat tatctttaat aaaagaaatg gtggtgtttg tatagggaat aaactgaaca
     3361 tcttgaatat tgtaattatc tctaaataat tgaacgctcg tttctgtatt agcaattaag
     3421 gtattagaaa tccctttttg aatcattcta ttaaaggccg aataaagaga gtctcgggct
     3481 tttgaatctt gactgagttt ttgaacggcg gtcgttgaaa gcctttgttt taatacatca
     3541 aacatacgac ttgcttgttg tgtaaacatt tgaacagagt cgggatcatt gctttcggca
     3601 atggcgtatt gtaatgatcc ataggcacca actttagata gaaactgttt caaatcgtct
     3661 agcgatctct tcgattcgtc atggttgaat tcaaagaccg cattgacttt gtctgctgct
     3721 ttttgccttt ctatttcaag ctcttgagct gttttaggaa catcaaaaga aatgggagca
     3781 ataatggttc ttggactcat ttgccctaaa tgaggcgtct ccgctttttg agcaagatct
     3841 ttatcgggaa acaaaaaaat cgcaagaaga gaaataaaga tccaacctac gacgatagaa
     3901 attttattct tattaatttt catttcattt gtcctgcatt tttttcgtaa gcggcaataa
     3961 tgtctttgac taggcgatgt ctaagaacat cggttccatt aaattcaaca tgagcgatgc
     4021 catgaatatt ttgtaataaa tcaatggcat gatttaatcc tgatacagtt cccttcccta
     4081 aatccacttg ggaagcatct cctgtaataa tggctttact atgagtgcct aatcttgtta
     4141 aaaacatttt catttgagct acggtagtgt tttgtgcttc atctaaaata ataaatgcac
     4201 gcttaagtgt tctacctctc atataagcta gaggcgccac ttcaatagcc cccgtctctt
     4261 gataacgttt tagcttctct ggagcgagta attctgcaag actatcgtga attgggcgta
     4321 gataaggtgc tattttttct ttgagatcac caggtaaata accgagagac tcaccagctt
     4381 cgactgctgg tcgaacaagg caaatacgtt cagcttcgtg cctttcaaga cttgctactg
     4441 caagagctac agctaaaaat gtctttccag tacctgcggg ccctttggca aaaatgatat
     4501 catttttttc aactgctttg attaaatcgt attgattttt agttcgaggc actacagcaa
     4561 aaccatagcg attacgaaga atgggctctg aagggatgct ttctagttcg tttgaagtta
     4621 cttcatactg aggttcaagc aatctacgca tccagtcgcc agtgatggag gtcccatttt
     4681 gggctgcaag tttaatctga tctaaaagcg taagagtcgc ttgaatattg cttctctctt
     4741 cttttgcttc tataaaaagg cctggaagtc ttgtttggat actcacacaa aaacggctct
     4801 ccaatatacg gaaaaccgtt tcattttctc cagccatgag tcttttcaga tcatctgaaa
     4861 ttggatagcg tataggttcg ctcattctcc agtttgctgc tcaacaggaa tacctagttg
     4921 atgtttggca tcaaaaattt cacgacgtaa cttatgattc tcttcagtaa gtgctgtcgc
     4981 ttctttttct gtgtctttaa gtttgtcttc agtaatcact tcttttttca cggctttctt
     5041 tgttcctgtg ccatcatctt cataataaac ttcgtcatca gaagaaccgc taccaccaga
     5101 agacccagcg caagcagcaa ataaaacaac ggtaccggca atgagtaata acttaaaatt
     5161 ctttaaaaga gacattattc aggctccatt ctagtctgtg ttggtctcaa atatatatag
     5221 atgaaatgat aaaaagtgta aaaaactaaa ttttttcttt atggaagcta ttaaacagtt
     5281 attgcaagac tattctgaag tttttatttc gactttttcc gaaaaaacag ctggaatata
     5341 tcaaaaacgt agacgagaat tactttctaa gctcgattct ttttgtgttt ttagtggtgt
     5401 agctgtagaa ccagggacag aagaagtctt tgcatctact tggacacgtt tcattcagga
     5461 tccttccttt ttattcttaa cgggaatcaa tcaagctggt tgtttcttat tgcttgatcc
     5521 tcttgctaaa gaggagtcta tgcgagaagt tctttttatt cctttgaaag acaaggataa
     5581 agagttttgg gtcggaaaac gtataggcta ttcagatgat ttatctgatt taaaaacgct
     5641 aactggattt caatccattc agccgtcgag ttctctttgg aataccatag aacggttatc
     5701 cttaagaaaa gagacccctt cttatgccta tgcttttttc tttcctgatt atcagttaga
     5761 ccacaatgcg aggtttgccc tcgaaatggg aactttttta gaaaagaaac aaaaaactct
     5821 tcgaagcgta agtgaaattc atttggaatt gagaatggtg cttgatcgtg agcgaatttc
     5881 attggtagag aaggcacaag aagcttctgc agaagctttt aaatccattc tatcacgttt
     5941 gcctttattt aaaaatgaaa gggctcttgg tttagctttg gattatgaac ttcaaagtcg
     6001 aggagatgga gatcttgctt ttccaacgat tgtggcgtct ggtaaaaatg catgttgttt
     6061 gcattactct aaaaaagatg aatctctaca ggaaaaagat ttagtccttt tagattttgg
     6121 aattcggata ggaactcaac atagtgacat ttcaagaact atccctgttt cgggccagtt
     6181 tgatccacta cagaagttac tctataatat agtgttagaa gctcagcttt ttcatgaaaa
     6241 gcaagttcgt ccaggagtga cattaagaga attagatcaa aatgtatggg attttattga
     6301 aaaatcatta aaaaatagtt tcacagataa ggggggcact tttgagcttc tatataataa
     6361 gcgtcctcat ggggtgagcc attttattgg agaacaagtt catgaagggg ctcctaaaag
     6421 taggtctcta gatgttgttc tgaaaccagg aatgatgatt tctaatgaac caggactata
     6481 tggccgtttc caaatgactc tagatggcgt tcattatgac cgtcttattg gaattcgtat
     6541 agaagatgat ttactcatta cagaaaatgg gtgcattaat atgtctatag atatcccaaa
     6601 acaagtggaa gaacttgagc gtctaattgc aaatggctca aattttttta atgtttaaaa
     6661 aatttttatg gtagactatg tggaaaatta aaggtacagt tcg
//
LOCUS       DAOOBB010000102         6688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_13542_length_6688_cov_4.836273, whole genome shotgun sequence.
ACCESSION   DAOOBB010000102 DAOOBB010000000
VERSION     DAOOBB010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 6688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6688
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13542_length_6688_cov_4.836273"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PLT31_05735"
     CDS             complement(<1..260)
                     /locus_tag="PLT31_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94674.1"
                     /translation="MRKQFDIIVYPVKEIEPPIEDPALEWFPSLNVNEALPNTTAEWI
                     IIIHHDINISREHLNQIAQACNDFPMIDCFAPSLYASSSETS"
     gene            complement(265..1140)
                     /locus_tag="PLT31_05740"
     CDS             complement(265..1140)
                     /locus_tag="PLT31_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94675.1"
                     /translation="MSLERSAGALPSTDPSIVQLNPAGLEIGEGKKNAVSVYWQTGAW
                     ADNQGIISYSRKLNAMTLQASYGWIRYGDIDGYDEFGNPTGQIHSPQSQLFTLTSSFP
                     LTHFRFGSTVKLATDKLSGEVGDRTAIALAFDWGFSWLSTSTRYGFALTARDFGSMIR
                     DYVDDSSNDSYAMAQTISIAAFLRPRTIPRLSLLMENSFPRYSQPTVSLGAEYILGKY
                     IFLRAGFNRTWLDLSRDAKELFASNSRPSESYESRLFSVGAGFLHEIFSLDYSFSYLT
                     QGLGLEHRFGLSFYF"
     gene            1326..1398
                     /locus_tag="PLT31_05745"
     tRNA            1326..1398
                     /locus_tag="PLT31_05745"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1359..1361,aa:Lys,seq:ttt)
     gene            1456..4797
                     /gene="mfd"
                     /locus_tag="PLT31_05750"
     CDS             1456..4797
                     /gene="mfd"
                     /locus_tag="PLT31_05750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HPW94676.1"
                     /translation="MMQNFSQFLQECSLDTLNIWDHHPARGTLHVSGLNLSAESIIIA
                     SRFLKNPENVVIITKDYKSSEEWMANLSSLVSEENIRFFPSIGLKPYEEKAPFDGVLE
                     ERLLFFRDIQTSGPLIVVCPIDALLLKLPAPGFLNKKIQSLKVGSILDPKALRPILLD
                     MGFKEQPIVSAVGEFSIRGCIVDINGFLYEHPIRIEFFGDEIESIRSFDIFTQRSLDT
                     MAAIDIYPMGEFNIAEKDKFHFTGDLNSYFWKRSAYESLTSSIFDYIKSANVVFDELS
                     SIAEHASSLRASYDVQYLEVAEGHFHDFTPDKIWWHFSHFSSFFANTFTLDFTRVQTN
                     ASNWMHYELRTQNFEDSGIDHVLKQIEEFTLEGGRVYIVAPTSGALHRLQHLFHEVEI
                     EGFLEASLTEGFWLTEEKVAFLTETRIFNRHSKKSRKKMISGSISNALLVESLSRGDL
                     VAHEDHGVGRYLGLVRVEVNGGMVDCALLEYQGGDRLKFPVADLQKIEKLQTPEDGEV
                     QLNRLGSKAFENLKKRVKQKVIQIAKELVELYAKRELTEGFAFAPDNKLQEEFEESFE
                     YEPTPDQTNAVLDIKKDMESKKPMDRLICGDVGFGKTEVAMRAAFKCILSKKQVVVLV
                     PTTILAAQHYENFRERFAAFPVNIDLVNRYRSVKDKKEVFEKLKKGAIDIVIGTHSLL
                     SQKIQFKDLGLLIIDEEQKFGVKQKELLREMRLSVDTLSMSATPIPRSLHLSMTGVRD
                     ISLINTPPMNRLPVETKLLKRNDVVLAQAVKDELARGGQVFIVNDRVQNILQLADDVE
                     SWAPEARVGVAHGQMADRDLERVMEAFISRHFDILVSTSIIESGLDVPNANTIIIMNA
                     HHFGISQLYQMRGRVGRSDVLAHAYLVTPAKGEISVDSAKRLQALEQFTDLGSGYQLA
                     MRDLEIRGAGNLLGSEQHGFIAEIGFETYVRLVKEAVEMIRGGTTEKPIQPRIEIGVD
                     AYLPEDWIEDGLSRITLYQRLARITSELEVKSIREEIQDRFGPIPEPAEMLFLITETA
                     LWAGRLRIQGIQQRRGMLALTFAEYPPVDPRILAEILSLSPYPMRYLGSTPLQAVIEL
                     GRGDSVALAKRVHETFKAFDSRTGG"
     gene            4800..6161
                     /locus_tag="PLT31_05755"
     CDS             4800..6161
                     /locus_tag="PLT31_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HPW94677.1"
                     /translation="MDIVKKIDALRAFYDSGKTLSFSYRLAQLKKLKKNIIKNEKQIE
                     EALKADLNKSDNEAYMTEIGITLSELTNMISGLSSYMKPKTVRTPLTLFKAKSFISPH
                     PYGVCLILSPWNYPFQLSINPLIGAIAAGNVALIKPSAYAPHTAALIERLIAETFEPD
                     YVQVVNGGREENQLLLDKKWDFIFFTGSPSVGRLVMEKASQHLTPIILELGGKSPAIV
                     TKSASLKSAAKRIAFGKVINAGQTCVAPDYVYVDASVKDEFLKHLTDYFKEFVPEGAK
                     TPDLPKIVNKKHFNRLLSLLEGETPYLKPDIDGETMSPAILYPASFDSKAMQEEIFGP
                     ILPVLTYSTEDEMIQTLQKKSKPLALYLFTNDSQTEKRVFEQLNFGGGCVNDTLVHLA
                     NHHLPFGGVGESGMGSYHGEDSFKAFSHSRSILKTSTLIDLPLRYRPYTSQKKSWLKR
                     FLK"
     gene            6285..>6688
                     /locus_tag="PLT31_05760"
     CDS             6285..>6688
                     /locus_tag="PLT31_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HPW94678.1"
                     /translation="MNSSDEIKLLLSKKELFLFDLDGTLFNTLGDLAPAVNYAMKHFG
                     LSTHSVDLIQTFIGNGALNLVRRAIAAHFIKNDLGEPKKAGSGCDFEKSIREEKIVAE
                     ALTREGYTEEKIAEIYKVYTEYYWDHCTENTEP"
BASE COUNT         2114 a         1201 c         1340 g         2033 t
ORIGIN      
        1 aaagaggttt cacttgagct tgcatataaa gaaggggcaa aacaatcaat cattggaaaa
       61 tcattacatg cctgtgctat ttgatttaaa tgctctcttg aaatattaat gtcatgatgt
      121 ataatgataa tccactctgc tgtggtatta ggaagcgctt cgtttacatt taaagaagga
      181 aaccattcta aagcgggatc ctctatcggt ggttctattt cttttacagg ataaacaata
      241 atatcgaact gttttcgcat gagactaaaa ataaaaactt aagccaaatc gatgttctaa
      301 accaagccct tgagtcaagt aagaaaacga ataatctaag gagaaaattt cgtgtaaaaa
      361 gcctgcacca acgctgaaaa ggcgagattc ataggattca ctggggcgag aatttgatgc
      421 aaatagttct tttgcgtctc gactaagatc aagccatgtg cgattgaatc ctgcacgtag
      481 aaaaatatat ttacccaaaa tatattccgc acctaaggag accgtgggtt gagaatacct
      541 tggaaaagaa ttttccatca gtaaacttaa gcgaggaatt gttcttggtc ttaagaatgc
      601 ggcaatggaa attgtttgag ccatcgcata agagtcatta gaagaatcat caacatagtc
      661 tcgtatcatg gaaccaaaat ctcttgcagt gagagcaaaa ccataacgag tgctagtgga
      721 taaccaagaa aagccccaat caaaggcaag tgcaatcgct gttctatcgc caacttcacc
      781 agaaagttta tccgtggcta gcttcactgt actaccaaaa cgaaaatgag ttaatggaaa
      841 agagcttgtt aaagtaaata attggctttg tggcgaatga atttgcccag tagggttacc
      901 gaattcatca taaccatcaa tatctccgta acgaatccaa ccgtaagaag cttgtaatgt
      961 cattgcattt aattttcttg aatatgaaat aattccttga ttatctgccc aagctccagt
     1021 ttgccaatag acggaaactg catttttttt gccttcccca atttctaatc ctgcaggatt
     1081 tagctgtact atacttggat ctgtagaggg gagagctcct gcagaacgtt ctaatgacat
     1141 gtttctagga ctttgaaaag ttctagaaaa agaaaaaact tcaagccctg cctcattctt
     1201 ttcgaaaaaa gaatatgaaa aaggagttat tagaccaaaa aataaagcaa aaaagaaaaa
     1261 aagtttaaaa aagtgcattt ttataattta caaaattcta aacgtttatt atctttggct
     1321 ttgtcgggtt aatagctcag ctggtagagc aacgcccttt taaggcgtgg gtcgaaggtt
     1381 cgaatccttc ttagctcaga aaaagccgat ccttttatag ggatcggctt ttttcttttt
     1441 atctttaagg cctttatgat gcagaatttt tctcaatttc ttcaggaatg ttctttagat
     1501 accttaaata tatgggatca ccaccctgca aggggtactt tacatgtcag tggtctcaat
     1561 ttatctgccg aatctatcat tatagcgagt cgttttctta aaaatccaga aaacgttgtt
     1621 attatcacca aagattacaa gagctcagaa gaatggatgg ccaatctatc gagccttgtt
     1681 tcagaagaaa atattcgctt ttttccttcc ataggcttaa aaccttatga agaaaaagcc
     1741 ccttttgatg gtgttttaga agaacgcctt ctttttttca gagatattca aacatcagga
     1801 cctctaatcg tagtttgccc tattgatgct ttattattaa agcttcctgc tcctggtttt
     1861 ttaaataaaa agattcaatc tctaaaagtc ggttcgattc tcgatccgaa ggccttaaga
     1921 ccaatcttat tagatatggg gtttaaggaa caacctatag tgagtgctgt aggggagttt
     1981 tctattcgcg gctgtatcgt ggatattaat ggattcttat acgaacatcc tattcgaata
     2041 gaattttttg gcgatgaaat agagtctatt cgctcctttg atatttttac gcaacgctct
     2101 cttgatacca tggcggcgat tgatatttat ccaatggggg aattcaatat tgctgaaaaa
     2161 gacaaattcc attttacagg agatctaaat agttatttct ggaaacgttc ggcttatgaa
     2221 tcgttaacgt cctctatttt cgattacata aaatcagcaa atgttgtttt tgatgaattg
     2281 tcttcaatag ctgagcacgc ttcttcttta agagcttctt atgatgttca atatctagaa
     2341 gttgctgaag gccattttca tgattttact cccgataaaa tttggtggca ttttagtcat
     2401 ttttcatctt tttttgcaaa tacttttact ttagatttta cacgtgttca aacgaatgca
     2461 tcaaattgga tgcattatga attacgtact caaaattttg aagattctgg aattgatcat
     2521 gtattaaagc aaatagaaga atttacgctg gagggcggac gagtttatat tgtggctcca
     2581 acttcaggtg cacttcatcg attgcaacat ttgttccatg aagtcgagat agaaggcttt
     2641 ttagaagcgt ctttaacaga aggtttctgg cttactgaag agaaggtagc gttcttaaca
     2701 gaaactcgca ttttcaatcg ccactccaag aagagtcgaa agaaaatgat ctctggctca
     2761 atctctaacg ccttattagt cgaatctctt tctagaggcg atctagtcgc tcacgaagac
     2821 catggcgtag ggcgttatct tggtcttgtt cgcgtggaag tcaatggcgg catggtcgat
     2881 tgtgcgttac tagagtatca aggtggcgat cgcttaaaat ttcctgtagc ggatttgcaa
     2941 aaaatagaaa agctacaaac ccctgaagac ggtgaagttc agctcaatcg attaggttct
     3001 aaagcttttg aaaacttaaa aaaacgcgtt aaacaaaaag tcatacaaat agcaaaagag
     3061 cttgtagagc tatatgctaa aagagaactt acagaagggt ttgcatttgc tccagacaat
     3121 aaacttcaag aagaatttga agaatctttt gaatatgaac cgacacctga tcagacaaat
     3181 gccgttttag atattaaaaa agatatggaa agtaaaaagc caatggatcg cctgatttgc
     3241 ggagatgttg gttttggtaa aacagaagtt gccatgcggg cggctttcaa atgcatctta
     3301 tccaaaaaac aagtcgtagt tcttgttcct acaacgattc tcgcggctca acattatgaa
     3361 aattttagag aacgttttgc tgcctttcca gtaaatattg atttagtcaa tcgttatcga
     3421 tccgtaaaag acaaaaagga agtttttgaa aaattaaaga aaggggctat tgatattgtc
     3481 attggaactc attctttact ttctcaaaaa attcaattta aagatttagg tcttttaatc
     3541 attgatgaag aacaaaaatt tggagttaag caaaaagaat tactccgtga aatgcgctta
     3601 tctgtagata cgctcagcat gagtgctaca ccgattccgc gttctcttca tctaagcatg
     3661 actggagtgc gagatatttc tcttatcaat acacctccaa tgaatcgcct tcctgtagaa
     3721 acaaaattat taaagagaaa cgatgttgtt ttagctcagg cagtaaaaga tgaattagct
     3781 cgtggcggcc aagtatttat tgtcaacgat cgagttcaaa atatcttgca attggctgat
     3841 gatgtagaat cttgggctcc tgaagcacgg gtgggtgtag ctcatgggca aatggctgac
     3901 cgagatctcg aaagagtgat ggaagctttt atttcaaggc attttgatat attggtgagc
     3961 acaagtatta ttgagtctgg actagatgtt ccaaatgcga atacgattat aatcatgaat
     4021 gctcatcatt ttggaatcag ccagctttat caaatgcgcg ggcgtgtcgg tcgttcggac
     4081 gttttagccc atgcctattt agtaactcct gcgaaaggtg aaatttcagt cgattcagct
     4141 aaaagattac aagctttaga acaattcacc gatttaggga gcggttatca gcttgcgatg
     4201 cgagatctag aaataagagg cgcaggaaac cttctaggat cagaacaaca tggctttatt
     4261 gctgaaattg gttttgaaac atacgtgcga ttggtaaaag aagcggtaga aatgattcgc
     4321 ggcggcacca cagaaaaacc aattcaacca cgtatcgaaa taggcgtaga tgcttattta
     4381 ccagaagatt ggatagaaga tggcttatct agaatcacat tataccagcg tttagctcga
     4441 attacttcgg aacttgaagt aaaatcaatt cgagaagaaa ttcaggatcg ttttggacca
     4501 attcctgaac ctgctgaaat gctcttttta attacagaaa cagctctttg ggctggtcgt
     4561 ctacgaatac aaggaattca gcaacgtcgc ggtatgcttg cgcttacctt tgcagaatat
     4621 cctcctgtag acccgagaat tttagctgaa attttatctc tatcgcctta tccaatgcgt
     4681 tatttaggct caacgccttt acaagccgtt attgaattag ggcgtggaga ttcggttgct
     4741 ctagcaaaac gagtgcacga gactttcaaa gcttttgatt caagaactgg agggtaaaaa
     4801 tggacatagt aaaaaagatt gatgctttac gagcatttta tgattcgggc aaaactcttt
     4861 ccttttctta tcgtttagct cagttaaaaa aacttaaaaa aaacattata aaaaatgaaa
     4921 aacaaataga agaagcgtta aaggccgatc taaataaaag cgataacgaa gcttacatga
     4981 cagaaatagg aattacgtta tcagaactaa cgaatatgat ttcgggctta tcttcttata
     5041 tgaaacccaa aacggttcgc acccctttga ccttattcaa agccaaaagt tttataagcc
     5101 ctcatcctta tggcgtctgt ttgattttat cgccatggaa ttatccattt caactttcaa
     5161 taaatccctt gatcggagcg attgccgcag gaaacgtcgc tttaataaag ccctcggctt
     5221 atgctcctca tacggctgct cttatagaaa ggctcattgc agaaaccttt gaaccagatt
     5281 atgtacaagt cgtaaatggt gggcgagagg aaaaccagct tttattagac aaaaaatggg
     5341 attttatctt ttttacaggt tctccttctg ttggccgtct cgtaatggaa aaagcaagtc
     5401 agcatcttac tccgatcatc ttagaacttg gagggaaaag cccagccatt gtcacaaaat
     5461 cggcatcctt aaaatctgca gcaaaaagaa ttgcttttgg aaaagtaatc aatgcagggc
     5521 agacttgtgt ggctcctgat tatgtttatg tagacgcttc tgtaaaagac gaatttctta
     5581 aacatttgac agattacttt aaggaatttg ttccagaagg cgcaaaaacg cctgatttac
     5641 cgaaaatcgt taataaaaaa cattttaatc gtttgctttc tttgcttgaa ggcgaaacac
     5701 cgtatctaaa gccagatata gatggtgaga ctatgagccc agccatttta tacccagctt
     5761 cttttgattc caaagccatg caagaagaaa ttttcgggcc cattttacct gttttgactt
     5821 acagcaccga agatgaaatg attcaaacgc ttcaaaaaaa gagtaagcct ctcgctttat
     5881 atctctttac caacgattct caaacggaaa aaagagtgtt tgaacagctg aattttggtg
     5941 ggggatgtgt caatgacact ctagttcatc tagcgaatca tcacttgcct tttggaggag
     6001 taggggaaag cggtatgggt tcgtatcatg gggaagatag ctttaaggct ttttctcact
     6061 ctagaagtat tctaaaaaca agtactttaa tagatcttcc tttacgatat cgtccttata
     6121 caagccaaaa aaagagctgg ctcaagagat ttttaaagta gattttcgag cttatttatt
     6181 tgctttttaa atccattatg ggttttatgg aaacgctttt gctgtgtgcg taataaattt
     6241 ttttggaacg gggatagttc gttttaattt tgtaaattag gcacatgaat tcttctgatg
     6301 aaattaaatt actgctctcg aaaaaagaac tgttcctttt tgatcttgat ggaacacttt
     6361 ttaatacgct tggcgacctc gctccagcgg tgaattatgc aatgaagcat ttcgggcttt
     6421 ctacgcacag cgtggatctt atacagacgt ttattggaaa tggagctttg aatctagttc
     6481 gacgtgcgat tgctgctcat tttattaaaa atgacttagg ggagcctaaa aaagcgggta
     6541 gtggatgcga tttcgaaaaa agtatcagag aagaaaagat tgtcgctgaa gcacttactc
     6601 gtgaaggtta caccgaagaa aaaatcgctg agatatacaa ggtttatacg gaatattact
     6661 gggatcattg caccgagaat acagagcc
//
LOCUS       DAOOBB010000103         6670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_13584_length_6670_cov_3.553741, whole genome shotgun sequence.
ACCESSION   DAOOBB010000103 DAOOBB010000000
VERSION     DAOOBB010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 6670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6670
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13584_length_6670_cov_3.553741"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..747
                     /locus_tag="PLT31_05765"
     CDS             118..747
                     /locus_tag="PLT31_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPW94679.1"
                     /translation="MITEITVFETTLQILSRGGWVLIPIFLLGWFAWFLLTERYFFYK
                     TLKGKAIQKKELLALDNGDLNYLLSSLKEGYLLKLLQAIKQYEPNGKKAVHYAMEAVQ
                     HDVHLSLSKSLKTISMCASLAPMLGLLGTVSGMVHTFEVIQLFGFGNPVLLADGISEA
                     LLTTQAGLLVAFPLMLALNYLTERIERLETKVWSQTLAFESRLFSQEDL"
     gene            744..1148
                     /locus_tag="PLT31_05770"
     CDS             744..1148
                     /locus_tag="PLT31_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPW94680.1"
                     /translation="MSFIKNKNRKASVIDISPMMDMVFILLIFFIVTSTFTRETGVDV
                     TKPKASSAKELAKESILIGVTRQGTIHINESQVNLSSLQTILRQMMAEAPDRPVIIVS
                     DRDAPSGRIVDILDECNIARVRKVSISAKRDE"
     gene            1155..1799
                     /locus_tag="PLT31_05775"
     CDS             1155..1799
                     /locus_tag="PLT31_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HPW94681.1"
                     /translation="MKSWILKWLRIPIALVLGLLINVAFFLAVPLINALFFNREEASK
                     KSMDVIAEVEVLVNEKPKQLEQKTIRTIVQPNTYKLNATQRESSRPSGFQMDLSLARG
                     EGGDGVAVGSGGMENVIYEAGEVDETARLLKEIQPKYPERAKKMGVSGYVKVYLVIDV
                     YGEVSQVRILTSEPTGYGFEEAALKAVREWKFEPAKLGSYPVSQKATKEFRFVP"
     gene            1789..2775
                     /locus_tag="PLT31_05780"
     CDS             1789..2775
                     /locus_tag="PLT31_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW94682.1"
                     /translation="MFHKSSLLFLIICVCGIGSSASFADDFFSKANALYDNGQYKEAV
                     PFYRAAIDEGRYEPFAWFNLGNTLVQLQKNEVALVAYRRTVELLPDFVKAWVLLGDLY
                     FLGDDLGNSIASYKRAIELGEDTDHIHYALGECFWKSGDYTLAQKHYERALYLNPDRM
                     GAWYGLADIYESLNDYEFAIKTLNNAIQQTASAGADAYFTISNYYQSMDSLSKALTAM
                     EDGLLIDPQNVSARRYLAQMYLKANSPWMAIFTIEEGLRYNKEKAVLLVDLGQIYFEQ
                     KRYEEAFECFLEAWKLGNSQGRIGAENVGHTWYNAGNQEQAEIIYKRIRERK"
     gene            2918..4300
                     /locus_tag="PLT31_05785"
     CDS             2918..4300
                     /locus_tag="PLT31_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94683.1"
                     /translation="MNRWFWALLGTFALFVGCDGSKPTEGEDLSSSSVSIIFISSAEV
                     NQWNQGNGTEESPYMINSADDLVALSNAVSQGVTYAGTFFKLGGNISLSGLSWTSIGV
                     WGEDDLGWKNKPFRGSFDGGGFTISNLTMDNASANYVGLFGLTKQAVIKNLKVESISL
                     SGNRIVGGLVAKADSTVISDCEVSVSLIGQERVGGVVGEMIAGSITNSIVSGSVEAKS
                     TVGGLVGVIAGGASVSGTNKATVTGTLQNTGGISGTVSGGVITDAINQGLVSGVLNVG
                     GIVGNVTMAGQVSWTSNQASVSASGANSFVGGVVGDLAAKSVLEASYNIGEVSSTGTY
                     AAGIAGKTTSSEIRDVFNHGKITAADFAGGLVGQCGENTIINRGYNKGSVSVTKNSAS
                     LLAKTLSTTEFSNLYYDNVLALVSNSEEGAVTSAELASANMATLLNAGETSVWVWNAG
                     EMGGYPYLAK"
     gene            complement(4414..5274)
                     /gene="lgt"
                     /locus_tag="PLT31_05790"
     CDS             complement(4414..5274)
                     /gene="lgt"
                     /locus_tag="PLT31_05790"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPW94684.1"
                     /translation="MDYSWWNLIPTKFDGIAIQLGNFPIHWYGVMYLFAFATCYFVMW
                     KINEKEKIGFNKEQFDSFATWIIAGILVGARLGYVFFYQPGYYLSHPLEIILPFRITD
                     AGFQFTGISGMSYHGGLIVAILFGIIGIKKNKMPFWTTLNLGFLAVPLGYSWGRFGNF
                     INGELYGEVTTSSIGMVFPMARDGLLHHPSQLYEMLFEGLILFTILFFLRKKDLFKNQ
                     MMSLYLIGYGTFRFFIEFFRKPDAHLLREDLFGMSRGQTLCSLMIIGGIVLFIIIRNK
                     EKNKTLNKKS"
     gene            complement(5287..6522)
                     /locus_tag="PLT31_05795"
     CDS             complement(5287..6522)
                     /locus_tag="PLT31_05795"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPW94685.1"
                     /translation="MKPLPYKVRLFNRILLIGFFIFLCTHCVFYFFSSEAPNSSLSTS
                     NYESLPDAPDSPLVPQADLAITGKLPEESPIDSTHILNQKDVYLAKKIDHLLRIYKPD
                     QALFLIVDAQENEILAWGQRSEGTIHTSPTFLSQSTFPAASLFKTVTLTAAFESKKYS
                     LDSPIPLIGRAHTLYRFQIQPPPNYSGNTVELQDAYAKSYNSPMAIVGYDVGTSFLKS
                     TAQKLGYNQKFENNIPEESKLNVPDTGYGIAEVSSGFTQSTTISPLLAAAQIRALLKQ
                     KPLEIPTATNITPYAPTKSKPVFDSLFSESTYYGIKEAMKRTVSHGTAKKNLSSRFIA
                     RRYREQLDIGGKTGSLDGSNPEGRYEWFAGFAKSRLESDKGIIIVVMQVHKEIRSQPA
                     TQVAGLLINYWAQQYLDLN"
BASE COUNT         2027 a         1047 c         1425 g         2171 t
ORIGIN      
        1 ggttgcaatc cccgtgcaac ttctacatac gtttttgtca aatcgtgcag atcgcttgac
       61 tggaaagatg gaaaaaatag gtcttgtatt attaaataga ctttggagca aaggttgatg
      121 attacggaaa ttaccgtatt tgaaactact ttacagatac tctctcgtgg tgggtgggtt
      181 ttaatcccta tttttttgtt aggttggttt gcttggtttt tattgactga acgatatttt
      241 ttttataaaa ctcttaaggg aaaagctata cagaaaaaag aattacttgc cttagataat
      301 ggagatctga attatttatt aagttcatta aaagaaggtt atttgttaaa attacttcaa
      361 gcgattaaac agtatgaacc caatggaaaa aaggctgtgc attatgctat ggaggctgtg
      421 cagcatgatg ttcatttaag cttatctaaa tctttaaaga caatatcgat gtgtgcttct
      481 ttagctccca tgcttggctt attaggcacc gtttcgggaa tggttcatac ttttgaagtg
      541 attcagcttt ttggctttgg aaatccagtg cttcttgcgg acggtatttc agaagccctt
      601 ttaacgactc aagctggtct tttagtggct ttcccattaa tgcttgcatt aaattatttg
      661 acagaacgaa ttgagcgttt agaaacaaaa gtttggagtc aaactcttgc ttttgaaagt
      721 cgtcttttta gtcaggagga cttgtgagtt ttattaaaaa taagaataga aaagcaagcg
      781 tcatcgatat ttcaccgatg atggatatgg tttttatact tttaatcttt tttattgtaa
      841 catctacatt tactcgtgaa acaggggtgg atgtgacaaa gccaaaagct agttctgcaa
      901 aagaacttgc aaaggaaagc attttaatcg gagtgactag acaagggact attcatatta
      961 acgaatccca agtcaacctt tcatctctgc aaaccatttt aaggcaaatg atggcagaag
     1021 ctcctgatag gcctgtgatt atcgtttctg accgagatgc tccttctggt cgaattgtcg
     1081 atatcttgga tgaatgtaat atcgctcgag tgcgtaaagt ttctatttcg gcgaagcgag
     1141 atgaataaaa aaatatgaaa agctggatat tgaaatggct tcgaattcca atagcactcg
     1201 ttttaggttt gcttattaat gttgcttttt ttctagcagt gccattgatc aatgcattgt
     1261 tttttaaccg tgaagaagct tctaagaaaa gtatggatgt tattgctgaa gtagaagttc
     1321 ttgtgaatga aaaacctaaa caattagaac aaaaaacgat tcgtacaatt gtgcagccca
     1381 atacctataa gttaaatgca actcaaagag aatctagccg tccaagtggc tttcaaatgg
     1441 atttatctct tgctcgtgga gagggtggag atggtgttgc ggtaggttct ggtggcatgg
     1501 aaaatgtgat ctacgaagct ggcgaagtgg atgaaacggc taggttatta aaggaaatac
     1561 aacctaaata tcctgaacga gccaaaaaaa tgggcgtatc aggttatgtt aaagtttatc
     1621 tggtgattga tgtttatgga gaggtttctc aagtgagaat tttaacttca gaacctactg
     1681 ggtatggttt tgaagaagcg gcacttaagg cggtgcgtga atggaaattt gaaccagcaa
     1741 aattaggctc ttaccctgtt tctcaaaaag ccacgaagga gttccgtttt gttccataag
     1801 agctctttat tgtttttgat tatctgtgtt tgcggtatag gatcatccgc ttcttttgcc
     1861 gatgatttct tttctaaggc aaatgcgttg tatgataatg gacaatataa agaagctgtc
     1921 cctttttatc gggcggcaat tgatgaaggg cgatacgaac cttttgcgtg gtttaattta
     1981 gggaatactt tagttcaatt acaaaagaat gaagtggcct tagtggccta tcgaagaacc
     2041 gtagaattgc tccctgattt tgtgaaagca tgggttcttc ttggtgatct ttattttttg
     2101 ggagatgatt taggaaattc tattgcatct tataaaaggg ccatcgaatt aggagaggat
     2161 acagatcata tacattatgc cttaggggag tgtttttgga aaagtgggga ctatactttg
     2221 gctcaaaaac actatgagag agctttgtat cttaatcctg atcgtatggg agcttggtat
     2281 ggtttagcag atatttacga atccttaaat gattatgaat ttgccataaa aacgttgaat
     2341 aacgccattc aacaaacagc ttctgctggt gccgatgcct attttacgat ttcaaattat
     2401 tatcaaagta tggattcttt gtcaaaagct ttaacggcga tggaagatgg attattaata
     2461 gaccctcaaa atgtctctgc acgtcgttat ttggctcaaa tgtatttaaa ggcaaattca
     2521 ccctggatgg cgattttcac gatagaagag ggacttcgat ataataagga aaaagcggtt
     2581 cttttagttg atttagggca aatttacttt gaacaaaaac gttatgagga ggcttttgag
     2641 tgctttttag aggcttggaa gctcggcaat tctcaagggc gaattggtgc agaaaacgtg
     2701 ggccatacct ggtataatgc aggaaatcag gaacaggcag aaataattta caaacgtatc
     2761 cgtgagagaa aatagtatat ttctcttagg ttctttagaa cgtgttcttt gatttaaata
     2821 ataagttgtt ctagtgaagt aattcatttg tagtaaattg tttttttaat ccgaaacaat
     2881 tttaaagaaa ccgaaagact ttttattagg agagaatatg aatagatggt tttgggcttt
     2941 attaggaacc tttgcattat ttgtgggctg tgatggttct aaaccgactg aaggtgagga
     3001 tttaagttca tcttctgttt ctatcatctt tatttcttca gcagaagtaa atcagtggaa
     3061 tcaagggaat ggtactgaag aatcccctta tatgattaat tctgcagatg atttagtcgc
     3121 tttatcaaat gcggtttctc aaggggttac ttatgcaggg acctttttta aattaggtgg
     3181 aaatatttcc ttaagtgggc tttcatggac ttcaattggt gtttggggtg aagatgatct
     3241 tggttggaaa aataaaccat ttagaggttc ttttgacgga ggtggtttta ccatttccaa
     3301 tctcacaatg gataacgctt ctgcaaatta cgtcggtcta tttggtttaa caaaacaagc
     3361 agtaattaaa aatctgaagg tagaaagcat cagtttaagc ggtaatagaa tagtaggtgg
     3421 tttagtagcg aaggcagatt ccactgtgat ttcagattgt gaagtgtctg tctctcttat
     3481 tggtcaagaa cgtgtaggtg gtgtagttgg ggaaatgatt gcaggttcaa tcaccaattc
     3541 tattgtaagt ggctccgttg aagcgaagag tactgtgggt ggtcttgttg gggtgattgc
     3601 aggtggtgct tcagtctctg gtactaataa agcaactgta actggaactc ttcaaaatac
     3661 gggcggtatt tctggaaccg taagtggagg tgtgattaca gatgccatca atcaaggatt
     3721 agtttctgga gttcttaatg taggcggtat tgttggtaat gtcactatgg caggtcaagt
     3781 ttcttggact tctaatcaag cgagcgtctc tgcttcagga gcaaattctt ttgttggtgg
     3841 tgttgtaggt gatttagctg ctaagtctgt tttagaggct tcttacaata taggcgaggt
     3901 ttcttctacg gggacttatg ctgcagggat tgctggtaaa acaactagtt ctgaaattcg
     3961 tgatgttttt aatcatggga aaataactgc tgctgatttt gctggtggtc ttgtaggaca
     4021 atgcggtgaa aatacaataa tcaatagagg ctataataaa ggctctgtga gtgtaacgaa
     4081 aaattcggct tctcttttag caaagactct ttccacaaca gaattctcaa atctatacta
     4141 tgataatgtt cttgccttgg tctctaattc tgaagaaggt gctgtaactt ctgcagaact
     4201 agcctctgcc aatatggcga cgttattaaa tgcaggagaa acaagtgttt gggtatggaa
     4261 tgctggtgaa atgggtggct atccctattt agcgaaataa ataaccttct tgttataagc
     4321 cgaaatgaaa agcgatgatt tcaatcatcg cttttcatgt ataaaaaata aagtggtgta
     4381 agcgttttac gcgaaagaat ttttgaaaaa tccttaggat tttttattaa gggttttatt
     4441 cttttcttta tttcgaataa tgataaagag aacgattcct ccaatgatca tcaaagaaca
     4501 aagggtttgc ccgcgactca ttccaaagag atcttctctc aataaatgcg catctggctt
     4561 tctgaaaaac tcaataaaga aacgaaaagt gccgtaccca ataaggtata gtgacatcat
     4621 ttgattttta aataaatctt ttttgcgaag gaagaagagt attgtaaata atataagccc
     4681 ttcaaaaagc atttcataaa gttgcgaagg gtggtgaagt aatccatctc ttgccatagg
     4741 aaaaaccatg cctatagagc ttgtagtgac ttctccgtag agttcaccgt tgatgaaatt
     4801 cccaaaacgt ccccaagaat aaccgagtgg aacggcaaga aaacctaagt ttaatgttgt
     4861 ccagaaaggc atcttattct ttttgatccc gatgattcca aatagaatag cgacaatcaa
     4921 accaccgtga taagacatgc ctgagatccc tgtaaattga aaccctgcat cggtgatccg
     4981 gaatggtaaa ataatttcta aaggatgact taaataatat cctggttgat agaaaaagac
     5041 gtatccaagt cttgctccca ctaaaatacc agcaataatc caagtggcaa aactatcaaa
     5101 ctgttcttta ttaaagccaa tcttttcttt ttcattgatt ttccacatca caaaatagca
     5161 tgtagcaaaa gcaaataagt acatgacccc ataccaatga attggaaaat tacctagttg
     5221 tatggcaatc ccgtcaaatt ttgtcggaat taaattccac catgaataat ccataatcac
     5281 ctctttttag tttaaatcta aatattgttg cgcccaataa tttattaata atccagcgac
     5341 ttgtgtggct ggttgagaac gaatttcttt atgaacctgc attaccacga taataattcc
     5401 tttatctgat tctaaacgag atttggcaaa tccagcaaac cattcgtaac gtccttctgg
     5461 attggatcca tctaaagaac ctgtttttcc tccaatatca agttgttctc tatatcttcg
     5521 agcaataaat cttgaggaaa gattcttttt tgccgtacca tgagaaactg ttcgcttcat
     5581 tgcttctttt attccataat aggtgctttc agaaaaaaga gaatcgaaga caggtttgct
     5641 tttcgttgga gcgtatggag taatgtttgt tgcagttggt atttcaagtg gtttctgctt
     5701 taaaagagct cttatttgtg cggcagcaag taaaggagaa atcgtggtgc tttgagtaaa
     5761 tccacttgaa acttctgcga tgccatatcc agtatcgggt acgtttaatt ttgattcttc
     5821 aggaatattg ttttcaaact tttgattata gcccaatttt tgagccgttg attttaagaa
     5881 agaagtacct acatcataac cgacaatagc cataggagaa ttataagatt ttgcataagc
     5941 atcttgtagt tctacagtgt ttcctgaata gttagggggg ggctgaattt gaaaacggta
     6001 taaagtgtgg gctcttccaa ttaagggaat agggctatct aaagagtatt ttttagattc
     6061 aaaagcggca gtgagtgtga ctgttttaaa taatgaagcc gctgggaaag tagattgaga
     6121 gagaaaggtt gggcttgtat gaatcgttcc ttcactgcgt tgcccccatg caagaatctc
     6181 attctcctgt gcatctacga ttaaaaaaag agcttgatct ggtttgtaaa tccttaaaag
     6241 atgatctatt tttttcgcta aataaacatc tttttgattt aaaatatgcg tactatcaat
     6301 ggggctttct tcaggaagct ttccagttat agcaagatct gcttgaggaa ctaaaggtga
     6361 atcaggtgca tcagggagtg attcataatt agaagtactt aatgaagaat ttggtgcttc
     6421 agagctaaaa aaataaaata cacagtgagt gcacaaaaat ataaagaatc caataagaag
     6481 aattcgatta aataaacgaa ctttataggg taagggtttc attcgagaga acctttaata
     6541 tcttaacaat agagcttgat ttgctctatg gaaagactaa agtttgaaaa tcgttttttt
     6601 gtaataatcg agttcagcaa tgctttctcg aatatcatta agagcctgat gcttttcttg
     6661 tttttgaaat
//
LOCUS       DAOOBB010000104         6646 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_13644_length_6646_cov_4.476104, whole genome shotgun sequence.
ACCESSION   DAOOBB010000104 DAOOBB010000000
VERSION     DAOOBB010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 6646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6646
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13644_length_6646_cov_4.476104"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="PLT31_05800"
     CDS             <1..376
                     /locus_tag="PLT31_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HPW94686.1"
                     /translation="LEQLVLDKETLFEKQKLSMVYANLVYNGQWFTPLRQALDAFMDE
                     TQKVVTGEVTLKLYKGNIIPAGMKSPYSLYDMDLGGFSDVEMYDQKDATGFIRCLGLP
                     MKTRALLLGKKTNVDFGKVQKF"
     gene            complement(465..1577)
                     /gene="mnmA"
                     /locus_tag="PLT31_05805"
     CDS             complement(465..1577)
                     /gene="mnmA"
                     /locus_tag="PLT31_05805"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPW94687.1"
                     /translation="MFLESVKKTIIAFMKIAVGFSGGVDSSMVALLLKQQGHEVVGIT
                     MSLWDPSFNIPLNGSKGCFGPEESKTLAVTQKTAEKLGISHAIIPLAKQYRQSVIEYF
                     REEYKAGRTPNPCVKCNQLMKFGFLLSSAKEMGIDFDYFATGHYARFSEEGHHLRTAV
                     DQTKDQTYFLSRLKKEQLKKVLFPLGNWKKEDIKKFALENGFEDLYHKSESQDFIQCG
                     DYSLLFNPEEIQPGSFVDKEGHVLGTHQGIIYYTIGQRKGLGIGGLKEPLFVTRLDPV
                     KNEVVLGTHDELFSKHLIGTDLNRLALEDHVTEGQATVKIRHGHKAVPATFSIEPENK
                     LHVVFDTPQLSITPGQIVTLYNNDIVLASAIIDHGY"
     gene            1593..1847
                     /locus_tag="PLT31_05810"
     CDS             1593..1847
                     /locus_tag="PLT31_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HPW94688.1"
                     /translation="MDISHWRKDIDELEDQIIALLNKRASYAVEIGKIKQEHGIPVLD
                     ATREKEILDRVALKTNGPLSPDSIRAIFKTIMEETRKVED"
     gene            1848..2954
                     /locus_tag="PLT31_05815"
     CDS             1848..2954
                     /locus_tag="PLT31_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HPW94689.1"
                     /translation="MQIRRLTFVGFGLLGASVAAAIKQAQKKIKLRAVSSPSTLKRAK
                     ELGLADEFYDYEDVDSWVEGSDVILLCGPILHILSTIKALSKTKPSHPVLVSDIGSTK
                     EKICEAGFQLPSPFSFVGGHPMAGSEKRSLEYNDPSIFENAYWFLCPPVNMPESTYLP
                     LSELVSFLGGQPVLFTPKEHDSTMAWLSHMPQMISSTLAGNIPSRILEKEHQHFAGRG
                     FRDMTRIAGSAWNMWHDILFTNKDAIEQALDEFSKGLKQTQTAIQALFENEEKVHEVF
                     VAGNSGRASLFAPGRNAADHFYEITVQLKDKPGAILSVMQPLAEEGINIRDIELMKVR
                     ENIAGTLLLAFKTEAEAAKAMKVLRYLEYEVKER"
     gene            2954..4309
                     /locus_tag="PLT31_05820"
     CDS             2954..4309
                     /locus_tag="PLT31_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HPW94690.1"
                     /translation="MAEFVVNPDSEMMEQALVLSLLVKGRTVLEDFQWTPRGLAFAEC
                     LREFGLSYELKGHQLVLEGMGFQYKAPMLLPYHFETHALILLWTLASKDTETIYTFAF
                     EEGNSLASEKTKALLLEYFLVVFEKESSDSIQFRFIEGAPKYKKNSQGDLPYLMRNRL
                     LLSALIQGEDLSFEERSALRDQWTRMMVYFGANLKYESKGMENMDELSRRIAKARGVK
                     LERTWSTTFSPTKILTAREYFVPGDVTELVALCLVVTLAKIPKDESIIIKNACINTGR
                     AGALTALKRMGALVETTSKRERYGDAFGDVEVKPLAGKRLQGRRFSEDLMASCMDEYP
                     LLALAACYAEGETILRIPPEYKASSRENLEWLAMNLRKTGVEVGVYDDGLVIRGKEEL
                     DGGEFDAANNPLTALTLFIVSLLGRGNSSIIGSEIMESTFPQISEKIKSIVQKGNHES
                     Q"
     gene            4296..5171
                     /locus_tag="PLT31_05825"
     CDS             4296..5171
                     /locus_tag="PLT31_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HPW94691.1"
                     /translation="MNHNRIGIVGWKDKSADLALALEMIAEWSKAHKEVSFCALESLK
                     GVSPKNIEIVTEDALRKCDLLLAIGGDGTVLSTARIALGTSIPILGVNAGHVGFLAET
                     RVEDLKTTLDLLQAGEFSTRNRMMLDAKIFREGNQLACETVLNEVHIRARASERMVNL
                     LVEYNGRYLTEYWADSLLVSTPTGSTAYNLSANGPIIHPSTSAFVLTPLAPSSLSVRP
                     LIIPASAICQISSVSDSSLNLVFDGRINYTLEARDKLVLSQSESVTTFIRMKNTGFVD
                     ALREKLGWTGKPKLA"
     gene            complement(5184..>6646)
                     /locus_tag="PLT31_05830"
     CDS             complement(5184..>6646)
                     /locus_tag="PLT31_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820357.1"
                     /note="decatenates newly replicated chromosomal DNA and
                     relaxes positive and negative DNA supercoiling; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HPW94692.1"
                     /translation="KEIYEVIKKAQNKDEMVNLIHEGLKPFVKKMLRKEVTREEILKL
                     AEIPIRRISRFDRKKADEALKELDLQIEETENHLIHLTDYAIAHFKNLLKKYGEGKER
                     KTTIGEFGKVNVVHVAIANQKLYVNRKEGFVGTGLKKEEYLFDVSSYDDIIVFKADGS
                     FKIVRVSDKDFVGKDVILIEKFNKDDERQIYNVIYQEGKDGVSYIKRFNVGGITRDKD
                     YLMGKGKPGSKILYISSNPNGEAEIVEVILKPRPRIKLNFEVDFSDVEVKGRNAIGNQ
                     VTKYAVKTVKRVRKGVSTLGARILYYDAPSGIVSTQKRGDCLGEFDENDKVLVVRENG
                     LARVYDMTDPLLVGTGIIHIQKFDPEQVYSILYFEGSNFNYMVKRFKLEACPMTTDFN
                     LLSDHPDTHLVEFFATKNAKVIMEYQVGREVQKETLDLTVVADVKGYKALGSKFTAKK
                     VKRTTRLIEEDEEEEEPEEKPSRKKAKDPYSVDMFE"
BASE COUNT         1926 a         1248 c         1346 g         2126 t
ORIGIN      
        1 ccttgaacaa ttagtattag acaaagagac cttgtttgaa aaacaaaaac tctctatggt
       61 ttatgctaac cttgtttata atggtcaatg gtttactcct ctacgtcaag ctttagatgc
      121 gttcatggat gaaacccaga aagtagttac aggtgaagtc actcttaaac tttataaagg
      181 caacattatt ccagctggaa tgaagagccc ttatagcctt tatgacatgg atcttggtgg
      241 cttctctgat gtagaaatgt acgaccaaaa agatgccact ggctttattc gctgcttagg
      301 cttaccaatg aaaacgagag cgttattgct tggcaaaaag acaaacgtag actttggaaa
      361 agtccaaaag ttttagtcgt tagtttataa acgaaaaaag ctcccgtaaa aacgggggct
      421 tttttttata aaaaaattaa ataatgcaaa tagacttaca cttgttagta tccgtgatct
      481 ataattgcag aagcaagcac aatatcatta ttatataaag taacgatttg acctggagta
      541 attgataatt ggggagtatc aaaaacaaca tgaagtttat tttctggttc aatactaaat
      601 gtcgcgggga cggctttatg cccgtgacga attttgacag tcgcttgccc ttctgtaacg
      661 tggtcttcta gagctaagcg attcaaatcg gtgccaatta aatgcttgga aaaaagctca
      721 tcatgagtgc ctaaaaccac ttcgtttttt actgggtcta aacgagtgac aaagagaggc
      781 tctttaagac caccaatacc taagcctttt ctttggccaa tcgtgtagta gataattcct
      841 tgatgcgtac ctaaaacatg gccttcctta tctacaaaag atcctggttg aatttcttct
      901 ggattgaaga gtaaagagta atctccacat tggataaagt cttggctttc acttttatga
      961 tagaggtctt caaagccatt ttctaaagca aattttttga tatcttcttt tttccaattg
     1021 cctaaaggga agagtacttt tttcagctgt tcttttttta agcgagagag aaaataagtt
     1081 tgatcttttg tttggtccac ggctgttctt aagtggtgcc cttcttcgga gaaccttgca
     1141 taatggcctg tagcaaagta atcgaaatcg attcccatct ctttggcgct acttaataaa
     1201 aagccaaatt tcatgagttg gttgcatttc acacaaggat ttggtgttcg accagctttg
     1261 tattcttctc gaaaatattc gatgactgat tgccgatact gttttgcaag aggaatgatt
     1321 gcatgagaaa tcccaagctt ttctgccgtc ttttgtgtga cggcgagcgt tttcgattct
     1381 tctggcccaa aacaaccttt acttccgttt aagggaatat taaaagaagg gtcccaaaga
     1441 ctcatcgtaa tccctaccac ttcatgacct tgttgcttta gaagcaaggc caccatggaa
     1501 gaatctacac caccgctaaa accgactgct attttcataa aagcaataat agtttttttt
     1561 acagattcta gaaaaatact acttttctag gtatggatat ttctcattgg cgtaaagata
     1621 tagacgaact ggaagatcaa attatagccc ttttaaataa gagagcttct tatgctgttg
     1681 aaatagggaa gattaagcaa gaacatggca ttcctgtgtt agatgcaaca cgagaaaaag
     1741 aaattctcga tcgagtcgct cttaaaacaa atgggccgct ttctccagat tctattcggg
     1801 caatttttaa aacgattatg gaagaaacaa gaaaagtaga ggattaaatg caaattcgcc
     1861 gccttacttt tgtaggtttt gggcttttgg gagcctctgt tgccgctgcg attaaacagg
     1921 cacaaaagaa gatcaaatta cgtgcggtga gctctccgtc tacgctcaag cgtgctaaag
     1981 aacttggtct tgccgatgag ttttatgatt atgaagacgt tgattcttgg gtcgaaggtt
     2041 ctgatgtgat attactttgc ggccctattc ttcatattct atcaacgatt aaagcgttat
     2101 cgaaaacaaa accatctcac ccagtacttg taagcgatat aggcagtaca aaggagaaaa
     2161 tctgcgaggc tggtttccag ttgccttctc ctttttcttt tgtcggtggt catccgatgg
     2221 caggttctga aaaaagatct ttagagtata atgacccttc gatttttgaa aatgcttatt
     2281 ggtttttgtg ccctcctgtg aatatgccag aatctactta tttgccttta tctgaattag
     2341 tctctttttt aggagggcaa ccagttctat ttactccaaa agaacatgat tctactatgg
     2401 cttggctaag tcacatgcct cagatgatta gttctacact tgcagggaat attccgtcgc
     2461 gcattttaga aaaagaacac caacattttg ctggtcgagg ttttcgtgat atgacgcgta
     2521 tcgctggttc tgcttggaat atgtggcatg atattttgtt taccaataaa gatgccatag
     2581 aacaagcact ggatgaattt tctaaagggc ttaaacaaac tcaaacagca atacaagcgc
     2641 tttttgagaa tgaagaaaaa gtgcacgaag tatttgttgc agggaactct ggccgcgctt
     2701 ctctttttgc tcctggccga aacgccgccg atcattttta tgaaattaca gtccagttaa
     2761 aagataagcc tggagccatt ttaagcgtaa tgcagccact tgcagaagaa ggtattaata
     2821 ttcgtgatat tgagcttatg aaagtccgtg aaaatattgc tgggacttta cttttggctt
     2881 ttaaaacaga agcggaagca gcgaaagcga tgaaagtact ccgctattta gaatatgaag
     2941 tgaaggaacg ttgatggctg aatttgttgt aaatccagat agtgaaatga tggaacaagc
     3001 tcttgttctt tctcttttag tgaaagggag aaccgtctta gaagattttc aatggacacc
     3061 tagagggctt gcttttgcgg aatgcttacg tgaatttggc ttgtcttatg aattaaaagg
     3121 gcaccaatta gtgttagagg gcatgggttt tcaatataaa gctcctatgc tactccctta
     3181 tcatttcgaa acgcatgcgc ttatcctttt atggacttta gcttcaaaag atacagagac
     3241 tatttatacc tttgcttttg aagaaggaaa ttctctcgcc tctgaaaaaa caaaagcatt
     3301 attgcttgag tattttttag tggtctttga aaaagaatct tcggattcga ttcaatttcg
     3361 atttatagaa ggggccccta aatacaagaa gaactctcaa ggagatcttc cttacttgat
     3421 gcgcaaccgc ttgcttttat cggccttgat tcaaggcgaa gatctctctt ttgaagaacg
     3481 ttctgcgtta cgagaccagt ggactcgtat gatggtctat tttggggcta atctaaaata
     3541 tgaatcgaaa ggcatggaaa acatggatga actttcacgt cgtattgcaa aagcaagagg
     3601 cgtgaaactc gaacggactt ggtctactac attttctccg acgaaaattt taacggctcg
     3661 tgagtatttt gttcctggag atgtcacgga actagtggct ctttgtttag tcgtgactct
     3721 tgcgaaaatt ccaaaagacg aaagtattat tattaagaat gcttgtatta atactggcag
     3781 ggcaggggca ttaacggctc tcaaacgtat gggcgcttta gtcgaaacca caagtaagcg
     3841 cgaacgctat ggcgatgctt ttggagatgt cgaagtaaaa ccacttgctg gtaaacgttt
     3901 acaagggcgc cgcttttcag aagatttaat ggcatcttgt atggatgaat accctttact
     3961 tgctttggct gcctgttatg cagaagggga gacgattctt cgaattcctc cagagtacaa
     4021 agcttctagc agagaaaatc tagaatggtt ggccatgaac ttgcgaaaaa caggagtcga
     4081 agtcggagtc tatgacgatg gtcttgttat tcgaggcaaa gaagaattgg atggcggtga
     4141 atttgacgct gctaataatc ctttaactgc attaacgtta tttattgttt cgcttttagg
     4201 tcgaggtaat tcatcgatca taggctcaga aattatggag tctacttttc ctcagatttc
     4261 tgagaaaata aaatcaattg ttcaaaaagg gaatcatgaa tcacaataga attggcatcg
     4321 taggctggaa agataaaagt gctgatttag cacttgctct tgagatgatt gccgagtggt
     4381 caaaagctca caaagaagtc tctttttgtg cattagaatc cttaaaagga gtctctccaa
     4441 aaaacattga gattgtcaca gaagacgcgt tacgaaaatg tgatttgctt ttggctattg
     4501 gtggagatgg tactgttctt tctacggcac gcattgcttt agggacttct attcctattt
     4561 taggcgtaaa tgcagggcat gtagggtttt tagctgaaac gcgcgtagaa gacctaaaaa
     4621 caactttaga tttattacaa gcaggggagt tctctactag aaatcgaatg atgctagatg
     4681 caaagatctt tcgtgaaggg aatcaacttg cttgcgaaac cgttttgaac gaagtgcata
     4741 ttcgtgcgag agcgtcagaa cggatggtga accttttagt cgaatataat ggacgttact
     4801 taacagaata ttgggctgat tcgctattag tatcgacgcc aacgggttcc acagcttata
     4861 atttatcggc aaatggccct attattcatc cttcgacatc ggcttttgta ttaacgcctt
     4921 tagcccccag tagcctttct gttcggcctt taattatccc agcaagtgcc atttgccaga
     4981 tttcttcggt gtcggattct tcgctaaatc ttgtctttga tggccgaata aactacactc
     5041 ttgaagctcg cgataagcta gttcttagcc agagcgagtc ggtaacgact tttattcgaa
     5101 tgaaaaacac aggctttgtc gatgctctga gagaaaagct aggatggact ggtaagccca
     5161 aactagctta gtttgaaaat aatttattca aacatatcta cagaataggg atcctttgct
     5221 tttttgcgcg aaggcttttc ttctggctct tcttcttctt catcttcttc gattaaacga
     5281 gtcgtgcgct tgactttttt agcggtgaat ttactcccaa gagccttata acctttgaca
     5341 tcagctacta ctgttaggtc tagggtttct ttttgcactt cacgccccac ttggtattcc
     5401 ataatcactt ttgcattttt agttgcaaaa aattctacca gatgagtgtc tgggtgatca
     5461 gagagtaaat taaaatctgt agtcatgggg catgcttcta atttaaagcg cttgaccatg
     5521 taattaaaat tggaaccctc aaaatagaga atcgaataga cttgttctgg atcgaatttt
     5581 tgaatatgaa taataccagt accaacgagt aatgggtcgg tcatatcgta aactctagct
     5641 aagccatttt ctcgaacgac taaaactttg tcgttttcat caaattctcc aaggcaatcg
     5701 cctctttttt gtgtagaaac aataccgctt ggggcatcat aataaaggat tctcgctcca
     5761 agagtgctaa cccctttacg tacgcgcttg accgttttta cggcatattt agtgacttgg
     5821 tttccaatgg catttctacc tttgacttca acatcactga aatcaacttc aaagtttaat
     5881 ttaatgcgag gacgaggttt taatatgact tctacaattt cagcttctcc atttggattg
     5941 ctagaaatat aaaggatttt ggagccaggt ttgcctttac ccataaggta atctttgtct
     6001 cgagtaatgc cacctacatt aaaacgttta atgtaagaaa caccatcttt cccttcttga
     6061 taaatgacat tataaatttg gcgttcatca tctttattga acttttcaat taaaatgaca
     6121 tctttaccca caaagtcttt atctgaaacg cggacaattt taaaagaacc atcggcctta
     6181 aatacaatga tatcgtcgta agaagaaaca tcaaataaat attcttcttt tttaagccct
     6241 gttcctacga aaccttcttt tcgattgacg tagagttttt ggttcgcaat cgccacatga
     6301 acaacattga ctttaccgaa ttcaccaatc gtggttttac gttctttccc ttcgccatat
     6361 tttttaagca aattcttaaa atgagcgatg gcataatctg ttaagtgtat gagatgattt
     6421 tcggtttctt caatttgaag atctaattct tttaatgctt catctgcttt tttacggtca
     6481 aaacggctga ttctacgaat aggaatttca gcgagtttaa gaatttcttc tcgagtcacc
     6541 tctttacgga gcattttttt gacaaagggt tttaagcctt cgtgaattag attcaccatc
     6601 tcgtctttat tttgcgcttt tttaatgact tcgtatatct cttttt
//
LOCUS       DAOOBB010000105         6603 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_13735_length_6603_cov_4.905315, whole genome shotgun sequence.
ACCESSION   DAOOBB010000105 DAOOBB010000000
VERSION     DAOOBB010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 6603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6603
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13735_length_6603_cov_4.905315"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PLT31_05835"
     CDS             <1..405
                     /locus_tag="PLT31_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94693.1"
                     /translation="GLVNNGMLFTVAIIGAISFTPVIILYFLIILGAPVGEYVMGGKN
                     RIIPKESRPPFITALIVQLILLFILLQVGGIIPFLLEPPLTCGIGYFFALYLLFNAAI
                     SLFSFSRKEKLFVAPLYLITSICFWATLLLNS"
     gene            451..2478
                     /locus_tag="PLT31_05840"
     CDS             451..2478
                     /locus_tag="PLT31_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HPW94694.1"
                     /translation="MDFLPISREELDECGWDYVDVIIVSADAYVDHPSFGHAVIARLI
                     EHEGFRVAILPQPNWRDDLRDFKKLGKPRYFFGISSGMDSMVNHYTAGKRLRSDDAFT
                     PGGKSGFRPDYATYVYTRILKELYPDVPVLIGGLEASLRRITHYDYWQDKLKPSILAE
                     TGADLLVYGMGEKPIKEILRLLKKGVPFSSIKNVPQTAYLADPNEIKKTKEWEDLYLS
                     SHEDCLENKQTQLKNFRTFELESNKMQSKRLLQNVNDKTVVINPPYPPLAYGELDESF
                     EYPYQRLPHPRYRKRGPIPAYEMIKHSITIHRGCFGGCSFCAINAHQGKFIASRSRES
                     ILRELEIITQMPSFNGTITDLGGPSANTYKMQGKDLERCKKCMRPSCLFPKICDNLDT
                     RHADLLSLYQEVQNHPKVKHLFIGSGVRYDILLQETNNVSLRKDHEAYTKTLIQNHVS
                     GRLKVAPEHTSDSVLRLMRKPAFSLFYDFKTIFERESDLVGKKQQIIPYFISSHPGCT
                     ESDMASLALETKQLGFKLEQVQDFTPTPLTIATEMYYAETLPNGTPIYIAKKPEQKKN
                     QQRFFFWYIRENRPWIKETLERLKLGKISRLLLSRSSLEEGRTYFPSQEAPSSKPTSP
                     RPSSPKSFSQNSASQRSASPKASFPKNSSHKQSFQNNKKSRSPYRRNSKSS"
     gene            complement(2605..4395)
                     /locus_tag="PLT31_05845"
     CDS             complement(2605..4395)
                     /locus_tag="PLT31_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94695.1"
                     /translation="MKQLVKIMGAFTLGFGLMSCGDSPVESKDLNVDTKGNLYISIRD
                     VNGTILTAGDSVKVTLLEIDKKPRFADSLGTITYKNLQVGSYQVLVEKEGYASMVCRT
                     SIDLASSDYIPVAADQTLAVDLHKIGASVTGTAVLQDAANINGLQTVPAEDATLRLSL
                     LGGACEYVESIVTVTTDEDGIYTFADLPEFASYSITPQAMTIDDELNSALSLTGTTGV
                     TGEYKKLSQISYSVTTDPFIVVSSNQSSVAIDDSIVIRFSKAVDVSRIRNGDIYVSNN
                     VGIVSSWSKDGKTLTVKAATGKWVYGSSFDLYFNVYSVNGIFISANSTNTASIRIFTD
                     GKLGPIKGLKAKNKMDTTIDYNSTAVELIWNKLSNAAKYEVYRRNSNDSSFVRVLTTS
                     SISDTSVYVTGNDFTDGKSVKFLVIGVNSVNSSPFVVDSAKNFYDKVKPTQSLLYSDQ
                     GTATSTTTEGNSAYDWVFESDAASVTAGSSDLNLNQLYDMVIDFSEPMDTSLFSVDEH
                     IEVTIDGSTNTSDKLTFKKQWSTDHSQLIISPRVAANKSGADAALRVTVSFADVKDKR
                     GNSIASKNNSVSVLYKHNTPVVLPPVEPEP"
     gene            complement(4671..5561)
                     /gene="ribF"
                     /locus_tag="PLT31_05850"
     CDS             complement(4671..5561)
                     /gene="ribF"
                     /locus_tag="PLT31_05850"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HPW94696.1"
                     /translation="MISRAITTGNFDGCHLGHQKLFSFLKEKAFAQNLKPTVVSFEPH
                     TRYLLGAPGKPALLTSTEEKKQFIESLGIDFFTIPFTHSLTQLPYSSFVEDYLEKDLQ
                     GKMFVFGYDHRMGAQGQGNYKTIINTFPHLQAFQMPAFYYKDELVSSSLVREAIENGQ
                     IERGNEFLGRPYRLTGTVIKGKQLGRTLGFPTANIKTAPFKLIPRFGVYVGKVTLADG
                     RYFRSVVNIGVQPSLENQELAIEAHLLNFSEDLYGVKVDLDLFSFIRPEQKFDSLDTL
                     VYQIKKDAAYTSELKNSLLP"
     gene            complement(5558..6460)
                     /gene="truB"
                     /locus_tag="PLT31_05855"
     CDS             complement(5558..6460)
                     /gene="truB"
                     /locus_tag="PLT31_05855"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPW94697.1"
                     /translation="MRSSGFVLLHKPAGVTSFQALFPLKKIFKTKRIGHAGTLDLRAS
                     GLIIAGVERATRLLPYIESADKVYTFRLHLGFLTDTLEWDGELIEQGKPISIGEADLE
                     SILPSFRGLIQQVPPNYSAIKIKGVRASDLSLQGKEFELKSRSVSIYDLKIIGKCEPP
                     ENASGEIFASFELECHCSKGTYIRSLCRDIGHALGTFGCVSHICRTQIGSITLSEAVL
                     PENLLESSIISPERILPYPVLTLSKEQAGMIRNGNWVPWKTPVENLSEENHVFVKNDM
                     GEIVSLCRFEPGRVIPKFFIGQEE"
     gene            complement(6457..6594)
                     /locus_tag="PLT31_05860"
     CDS             complement(6457..6594)
                     /locus_tag="PLT31_05860"
                     /inference="COORDINATES: protein motif:HMM:NF014130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94698.1"
                     /translation="MRQFLGKGIKIRKIPELQFVLDENLEHALHIDSILADLKNQGDL
                     D"
BASE COUNT         2134 a         1287 c         1139 g         2043 t
ORIGIN      
        1 ggactagtta ataatgggat gttattcact gttgctatta ttggtgctat ttcatttacc
       61 cctgtgatca ttctttattt cctaatcatc ttaggcgccc ctgtcggtga atatgtcatg
      121 ggaggaaaaa accgaatcat tcctaaagag tcaagacccc catttattac agctcttatt
      181 gttcaactga ttttgctttt tatcttatta caggtgggag gcattattcc tttcttatta
      241 gaaccgcctc tcacttgtgg cataggttac ttttttgctc tatacttgct attcaacgca
      301 gcgatttctc ttttttcttt tagccgcaaa gaaaagcttt tcgtagctcc tctgtattta
      361 atcacttcca tctgcttttg ggcaacgcta ctcttgaatt catagagaat ctaatttgga
      421 tgttgttcat tcttttctat cttagaggct atggattttt tacctattag tcgcgaagaa
      481 cttgatgaat gcggttggga ctacgttgat gttattattg ttagtgctga tgcttatgta
      541 gaccaccctt cttttggtca tgctgtcatt gcgcgattaa ttgaacacga aggctttcgt
      601 gtggctattt taccacagcc gaattggaga gacgatcttc gagatttcaa aaaactaggc
      661 aaacctcggt atttttttgg tatttctagt ggcatggata gcatggtcaa tcattatacc
      721 gcaggcaaac gccttcgtag tgatgacgcc tttacccctg gtggaaaatc aggatttcgc
      781 ccagattatg ccacctacgt ttacactcga attcttaaag aattataccc cgacgttcct
      841 gtcttaattg gtggattaga agcaagctta cgacgtatca cgcattatga ttactggcaa
      901 gataaactaa aaccaagtat cttagcagaa acgggagcag accttttagt ctacggcatg
      961 ggcgaaaagc ccatcaaaga aattcttcgt ttgcttaaaa aaggcgttcc gttttcaagt
     1021 ataaaaaacg ttccacaaac cgcttactta gcagatccaa acgagataaa gaaaacaaaa
     1081 gaatgggaag atctttatct tagtagtcat gaagattgtt tagaaaacaa gcaaacgcaa
     1141 ttaaaaaatt ttcgcacgtt tgagttagaa agcaataaaa tgcaatctaa acgattgctt
     1201 caaaatgtaa atgacaaaac ggttgtaata aatcctccat accctccttt ggcttatgga
     1261 gaacttgatg aaagttttga atacccttac caaagattgc ctcacccacg ttaccgtaaa
     1321 cgcggcccga ttcctgctta tgaaatgatt aagcattcta ttactatcca tcgagggtgt
     1381 tttggtgggt gtagtttttg tgctataaac gctcatcaag gtaaatttat tgcaagtcga
     1441 agcagagaaa gcattcttcg cgaacttgaa attattactc agatgccttc ttttaatggc
     1501 acgattaccg atttaggcgg gccatcggcc aatacctata aaatgcaggg gaaagatttg
     1561 gaacggtgca aaaaatgcat gcgtcctagt tgccttttcc cgaaaatttg cgataacctt
     1621 gacactcgtc atgccgacct tttaagcctt tatcaagaag tgcaaaatca ccctaaagtc
     1681 aagcatctct ttattggaag cggggttcgc tatgatattc ttcttcaaga aacaaacaac
     1741 gtctctttaa gaaaagatca cgaagcttat actaaaacgc tgattcaaaa ccatgtgagc
     1801 ggtcgtctta aagtggcccc agaacatact tctgattcag ttcttcgcct aatgcgtaag
     1861 ccagcatttt ctttatttta tgatttcaaa acgattttcg aaagagaaag tgaccttgta
     1921 gggaaaaaac agcaaattat tccttatttt atttctagtc acccaggttg tacagaatcg
     1981 gatatggcct ctcttgcact tgaaacaaaa cagcttggtt ttaaattaga acaagtacaa
     2041 gattttactc ctacgccttt gacgattgca acagaaatgt attacgcaga aactcttcct
     2101 aatgggacgc ctatttatat agcgaaaaag ccagagcaaa aaaagaacca acagagattt
     2161 ttcttctggt atattcgtga aaatcgtcct tggattaaag aaacgttaga acgattaaaa
     2221 ttaggcaaaa tttctagact gttactttca agatcttctt tagaagaagg tcgaacttat
     2281 tttccaagcc aagaggctcc ttcttcaaag ccaacatccc caagaccatc ttcaccaaag
     2341 tctttctctc aaaattctgc atcacaaagg tctgcttcac caaaagcatc ttttccaaaa
     2401 aattcttctc ataagcaatc tttccaaaat aacaaaaaga gtcgttctcc ttatagaaga
     2461 aatagtaagt cttcttaact tatattttca attctcacta caattccata ttatactttt
     2521 agcaataaaa acaccccctc attagagggg gtgtttttat tttattcaat ctcttattga
     2581 agcttattca gcatctggag ttgattatgg ttctggttca acaggaggaa gtacaacagg
     2641 agtattgtgt ttgtacaata cgctcacgct gttattcttc gaagcaatag aattaccacg
     2701 tttatcttta acatcagcaa aagaaacggt gacacgtaaa gcagcgtcgg caccagattt
     2761 attagctgca acacgaggac taataattag ttgactatga tctgtagacc attgtttctt
     2821 aaaggttaac ttatctgaag tgttagttga accatcaata gtgacttcaa tatgttcatc
     2881 tactgagaat aaagaagtat ccattggttc actaaaatca atcaccatat cgtataattg
     2941 attaagattt aaatcactgg aacccgctgt tacagaagcg gcgtcacttt caaataccca
     3001 gtcataagca gaattaccct ctgtagtagt tgaagtagct gtaccctgat cggagtataa
     3061 taatgattga gttggcttta ccttatcata gaaatttttt gcactatcta caacaaatgg
     3121 agaggaattc actgaattca ctccgatcac taaaaattta accgattttc catcagtaaa
     3181 atcatttccc gtgacataaa ctgaagtatc actaattgat gaggttgtca aaacacgaac
     3241 aaagcttgaa tcattagaat ttctacgata gacttcatat ttagcagcat ttgataattt
     3301 attccaaatt aattctactg ctgtactatt gtagtcaatt gtggtatcca ttttattttt
     3361 tgccttcaaa cccttaattg ggccgagctt accatctgtg aaaatgcgaa tggaagcagt
     3421 gtttgtagaa ttagcagaaa tgaaaatacc gttcacagag tatacattaa aataaagatc
     3481 gaatgaagag ccgtaaaccc atttgcctgt tgcagccttt acagtaagag tcttaccatc
     3541 ttttgaccaa gaggaaacta tacccacatt attacttaca taaatatctc cattacggat
     3601 acggcttaca tccactgctt tggagaagcg gatcacaatg gagtcatcaa tagctacaga
     3661 gctttgattt gaagaaacaa cgatgaaagg atctgtagtt acagaatagg aaatctggga
     3721 aagcttttta tattcaccag ttacgccagt agttcctgta agactaagtg cggagttcaa
     3781 ctcatcatca atagtcattg cttgaggagt aatggagtag gaagcaaatt caggaagatc
     3841 agcaaatgta tatataccat cttcatccgt agtcacagta acaatagatt caacatattc
     3901 acatgcacca ccaagtaaag ataagcgcaa agtagcatct tcagcaggaa cggtttgtaa
     3961 tccattgatg ttagcagcat cttgaagtac agcggtacct gtaacagaag cacctatttt
     4021 gtgtaaatcc acagctagag tctgatctgc agccactgga atataatcag aagaagctaa
     4081 atcaatgctt gttctacata ccatagaggc ataaccttct ttttcaacca atacttgata
     4141 agagccgact tgaagatttt tataggtgat ggttccgagt gaatcagcaa aacgaggctt
     4201 tttatctatt tcaagtaacg ttaccttaac ggagtcacct gccgttaaaa tagtaccgtt
     4261 tacatcacga atagagatat aaagattacc ttttgtatct acatttaaat cttttgattc
     4321 tacgggacta tctccacagg acatgagccc gaagcccaat gtgaatgcac ccataatttt
     4381 gactagctgt ttcatttgtt ctcctattta aacaagcgta gccaaatata atattaatgt
     4441 aagattacag caagcttatt ttatgaaata gacgcatcac ttaggaaata attaagcata
     4501 aactcatttt ttaatttcct ccctaatcaa ttttttatta aaaaaacaaa aaaaaaacag
     4561 tttttttaag tatttattaa caatcgaagc gatagaatag ttttcaatcg tttttttaat
     4621 aaaataaaag tgtgtcttta atcactttcc gaattagtga gacaatgata ttaaggcaaa
     4681 agactgtttt ttaattcaga agtatacgca gcgtcttttt tgatttgata aactaacgtg
     4741 tccaacgaat caaatttctg ttctggacga ataaaagaga ataaatctaa atccactttc
     4801 acgccatata ggtcttctga aaaatttaat aaatgtgctt caatggctaa ctcttgattt
     4861 tccaaagaag gctgtacgcc tatatttaca actgaacgga agtaacgccc atctgcgaga
     4921 gtcaccttcc ctacataaac tccaaaacga ggaattagtt tgaatggagc tgtttttata
     4981 ttcgctgtag gaaacccaag tgtacgcccc aattgttttc ccttgattac ggttccagtc
     5041 aatcgataag gacgccctaa aaactcattt cctcgctcaa tttgaccgtt ttcaatggcc
     5101 tctcgaacaa gagaagaact gactaattca tctttataat aaaaagctgg catttgaaac
     5161 gcttgtaagt gaggaaaagt atttataatc gttttataat ttccttgccc ctgagccccc
     5221 attcgatgat catagccaaa aacgaacatt tttccttgaa gatctttttc aagataatct
     5281 tcaacaaaag agctataagg aagctgcgtt agcgaatgcg taaaaggaat ggtaaagaaa
     5341 tctatgccaa ggctttcaat aaattgtttt ttttcttccg tagaggttaa taaagctggt
     5401 ttccctggag cccctaaaag ataacgtgtg tgcggttcaa aagagaccac cgttggtttt
     5461 aaattttgtg caaaagcctt ttcttttaag aaagagaaaa gtttttgatg ccccaaatga
     5521 caaccatcaa aattgcctgt ggtaatcgca cgagaaatca ttcttcttga cctataaaaa
     5581 attttggtat cactcttcct ggttcaaatc ggcataaaga gacaatctct cccatgtcat
     5641 tttttacaaa aacatgattt tcttcggata aattttctac tggtgttttc caaggaaccc
     5701 aatttccatt tcgaatcatg cctgcttgtt cttttgaaag agttaaaaca ggataaggta
     5761 aaatacgctc tggagaaata atagatgact ccaatagatt ttctggtaat accgcttctg
     5821 ataaagtaat agaaccaatc tgtgttctac aaatatgaga aacacaacca aaagtaccta
     5881 aagcatgccc aatatctcga caaagagaac gaatataagt tccctttgaa caatgacatt
     5941 ctaattcaaa agaggcaaat atttcaccac tcgcgttttc aggaggttcg cattttccaa
     6001 taattttcaa atcgtaaatt gaaaccgatc gtgatttcaa ttcaaattct ttaccttgta
     6061 aacttaaatc agatgctcga acacctttta ttttaatcgc actgtaatta ggaggaactt
     6121 gttgaataag acctctaaat gaaggtaaaa tagattctaa atctgcttct ccaatagaaa
     6181 taggttttcc ctgttctatt aattctccat cccattccaa agtgtctgtc aaaaatccta
     6241 aatgtagacg gaaggtgtat actttatctg cagactcaat ataaggaagt aaacgagtcg
     6301 cacgttctac cccagcaata attaacccag aagctctcaa atcaagagtc ccagcgtgtc
     6361 caatacgttt agttttaaaa attttcttta atgggaacaa cgcttgaaag gatgttacac
     6421 ccgcaggctt gtgaagtaaa acaaatccag aagacctcaa tcgaggtctc cctgattttt
     6481 gagatctgct aagatagaat caatatgaag cgcatgttct aagttttcat caagtacaaa
     6541 ttggagttca ggaattttac gaattttaat tcctttccca agaaactgac gaataaaacc
     6601 agc
//
LOCUS       DAOOBB010000106         6584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_13783_length_6584_cov_4.523817, whole genome shotgun sequence.
ACCESSION   DAOOBB010000106 DAOOBB010000000
VERSION     DAOOBB010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6584
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13783_length_6584_cov_4.523817"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PLT31_05865"
     CDS             <1..558
                     /locus_tag="PLT31_05865"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HPW94699.1"
                     /translation="RSWGLSIDLFKNFRPWMVAMQISAMAIERTGFSSEWGIEYMLMD
                     QAVLLGKEIVSLETPATQIEALATPSDTLGVYYLETTLKEISQIDSLLSQIAFAWQTG
                     NRSKLQSLLNPKSQLKSDKEKEMDNVIKEKIYSERNIGMVNRIESFIKENKKFFIAVG
                     VAHLIEDDDNILELLKNKGFKVDSF"
     gene            complement(568..1929)
                     /locus_tag="PLT31_05870"
     CDS             complement(568..1929)
                     /locus_tag="PLT31_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPW94700.1"
                     /translation="MTLVSSPRTKILLLSEENPIASFILDVLSSVDYEVLFSEKVILH
                     PHFVPMILIVNLESPFIQPDSIEKLQQFYTDSLLIGFSSQPLNSYPVLIQNTFTVFLD
                     IEHLKLQLMSHILKLKEVYVYKKRFKESLKKLVGHSEPMQVLYKKIEQILPHKGTVLI
                     QGASGVGKELVARAIASVQPHLVIVNCSAIPENLFESELFGHARGAFTGATGDRVGLF
                     EEADNGALFLDEIGDMPLPMQTKLLRALQEGEIRRVGSNITKKVSVRLIAATNRNLKE
                     EVLKGRFREDLFYRLNVIPIEIPPLKERLEDIEDLAPYFIKRYAPLGKSYSLSKEALK
                     KLKEHDWPGNVRELENTIHRAISFTETPEIQSESIIIDSVLSKKNHDEKDKAWEKLNY
                     KEFKEYQLEEERDYIRQKFLENGSSITKTAAAFGMLRTALHNRATRIGLHFRSIEKDN
                     TSP"
     gene            complement(1940..2737)
                     /gene="proC"
                     /locus_tag="PLT31_05875"
     CDS             complement(1940..2737)
                     /gene="proC"
                     /locus_tag="PLT31_05875"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HPW94701.1"
                     /translation="MNQKIFFIGVGNMGGAILKGLLQAKKSPQDILFFEPNDLTAKEY
                     ESFGALRVSSFAEGINEAETAFICVKPQIFNTVVSSWKKDLQQVSKKVFISIMAGIPS
                     TTLKNELGSHEVLRVMPNLPLTVGKGAIALSADFVSEETLVLAESIFKNVGITVRVQE
                     SLMDAVTGLSGSGPAYVFEFIEGLIMGGVKMGLTRSASKELALATLEGSLELLKQSGK
                     EPQALTAMVSSPGGTTIAGLQVLEEKAFRGVLMRTVEAATLRSKELG"
     gene            complement(2754..4049)
                     /gene="rho"
                     /locus_tag="PLT31_05880"
     CDS             complement(2754..4049)
                     /gene="rho"
                     /locus_tag="PLT31_05880"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HPW94702.1"
                     /translation="MIQAPPADPEKVALAVQKWKQLRNMPMFELQRQALELEIPDVKR
                     LRKQALIYRILGATAGEDVPIYTEGVLEILNEGYGFLRSPDHNYLAGPDDIYISPNQI
                     KRFELKTGDSITGQVRQPRDNEKYFALMRIDTINFDPPEKAKRRVSFEYLTPIHPFQK
                     FNLEWNKEELSTRIMDLFTPIGKGQRGILLAPPRTGKTVLLQNVANAIAANHPEVKLL
                     VLLIDERPEEVTDMRRHVKGEVLASTFDEPPERHVQVADMVLEKAKRLVEHGNDVVIL
                     LDSITRFARANNVVIPHSGKILSGGVDAMALQRPKRFFGAARKIENGGSLTIIGTALI
                     ETGSRMDEVIFEEFKGTGNMELVLDRRISEKRIWPAIDIFKSGTRKEELLLSEDELRR
                     VWILRRYLQDHTSTEIMEFMREKMLNAITNKDFLSSMNG"
     gene            4710..5219
                     /gene="folK"
                     /locus_tag="PLT31_05885"
     CDS             4710..5219
                     /gene="folK"
                     /locus_tag="PLT31_05885"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HPW94703.1"
                     /translation="METLERVFIALGSNISPRGPRLKAARQMLQGIAAGGWKESAIYE
                     TPPVGPEGQDNYFNQVVSFWYSKGKGRLLNYLKGAELLLGRKPRGHWNSREIDLDLLY
                     YGEIVSSGRPTLPHPEVQKRQFVLIPLCDIAPEWKDPLTGRTLQEILFELRRREGDLG
                     FKIVDPEDS"
     gene            5220..5867
                     /locus_tag="PLT31_05890"
     CDS             5220..5867
                     /locus_tag="PLT31_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HPW94704.1"
                     /translation="MILNPDIRFLAIEGVIGVGKTSLAKVLAERLNATLLEEMFEENP
                     FLEKFYQQPEEYAFQTQLFFLLSRHRQLLDSFIQNDLFRHLIISDYSFDKDRIFALQN
                     LSEDEMYMYDAVLKSLDNNVPRPDYIIYLQASVETLITRIKGRNRPMEKGIDGHYLRD
                     LQERYDHHFWHYNDCPVLIINTDKVDFVNNENHLKQILNVIQTWPSQTTYFIPEG"
     gene            5873..>6584
                     /gene="panC"
                     /locus_tag="PLT31_05895"
     CDS             5873..>6584
                     /gene="panC"
                     /locus_tag="PLT31_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HPW94705.1"
                     /translation="MKVIKTIASLRRTLSTYNKAGKKIGLVPTMGALHEGHMSLVDKS
                     VEEMDVTIVSVFVNPIQFGKNEDLDKYPRRLEADASLASEHGADFIFAPSVAEMYPSG
                     NSSTLIRNEELEGLYCGAYRPGHFRGVLTVVAKLFMITQADKAYFGLKDYQQVFLIEK
                     MVEDLNIKTKIVRIPIVRGPSGLALSSRNEYLTDEEKANAVALFEGLTAAKKAYSEGE
                     RSVAKLRDSVIRYILMKRG"
BASE COUNT         2016 a         1170 c         1268 g         2130 t
ORIGIN      
        1 cgttcttggg gtttgtcgat cgatcttttt aagaatttta ggccttggat ggtagcgatg
       61 caaattagtg cgatggctat tgagcgtaca ggcttttctt cagaatgggg tatcgaatac
      121 atgttgatgg atcaggctgt tttattaggt aaagaaatcg taagtttaga aacacctgca
      181 acgcaaatag aagcgttggc gactccatca gatactctag gtgtttatta tttagaaacg
      241 acactaaaag aaatttctca aatagacagt ctcctttctc agatcgcgtt tgcttggcaa
      301 actggaaatc gttcaaaatt acagtcttta ttgaatccga aaagccaatt aaaaagtgat
      361 aaagaaaaag aaatggataa tgttattaaa gaaaaaattt attcggaaag aaatattgga
      421 atggtaaatc gaatagagag ctttataaaa gagaataaaa aattttttat tgctgtaggt
      481 gtcgctcatt tgattgaaga tgatgacaat atacttgaat tgctcaaaaa caaagggttt
      541 aaggtcgact ctttttaaaa tattgaatca agggcttgtg ttgtcttttt caatcgatct
      601 gaaatgcaag ccaattcgtg tggctcgatt atgaagagct gttcttagca ttccaaaagc
      661 ggccgctgtt tttgtaatag aagaaccatt ttctaaaaat ttttgacgaa tataatctcg
      721 ctcttcttct aattgatatt ccttaaattc tttatagttt agtttttccc aagctttatc
      781 tttttcatca tgattctttt tagataaaac ggaatcgata atgatacttt cggattgaat
      841 ttcaggcgtt tctgtaaaag aaatggctcg atgaattgta ttctcaagct cgcgaacatt
      901 ccctggccaa tcatgttctt ttagcttttt tagtgcttcc ttagataatg aataagactt
      961 gccaagggga gcgtaccgct ttataaaata gggagctaaa tcttctatat cttcaagacg
     1021 ctcttttaat ggaggaattt caataggaat tacatttagt ctatagaaca aatcttctcg
     1081 gaaacgtcct ttaagaactt cttctttcag attcctgttt gtggcagcaa tcagtcggac
     1141 ggaaactttt tttgtgatat tggaaccgac tcgacgaatt tctccctctt gaagagcacg
     1201 cagcaatttc gtttgcatag gaagaggcat atcgccaatt tcatcaagaa ataaagcccc
     1261 attatccgct tcttcaaaaa gcccaacgcg atctccagtg gctccagtaa aagcacctct
     1321 agcatgacca aaaagctcgc tttcaaagag attctctgga atagcactgc aattaacaat
     1381 aaccaagtgt ggttgcacac tggcaatcgc tctggcaacg agctcttttc caactcctga
     1441 agctccttga attaaaacag tccctttgtg cggcagtatt tgctctattt ttttatataa
     1501 aacttgcatt ggctcgctat gtccaacaag tttttttaga ctttctttaa aacgtttttt
     1561 ataaacataa acttctttga gctttaaaat atggctcatt aattgaagtt ttaagtgttc
     1621 aatatctaag aagacagtaa atgtattctg aattaaaaca gggtaagaat ttagtggctg
     1681 agaactaaaa ccaattaata gagagtctgt ataaaattgt tgtagctttt cgatcgaatc
     1741 gggttgtata aaaggtgatt ctaaattaac gatcaaaatc attggaacaa aatgagggtg
     1801 taatatgact ttttctgaaa agagaacttc ataatcaacg ctagaaagaa cgtctaaaat
     1861 aaatgaagcg attggattct cttcactcag aagtaatatt ttagttcttg gagatgacac
     1921 caatgtcatc actgcctcat tagcctaatt cttttgatct taaagtagct gcttctacag
     1981 tgcgcattaa cacgccacgg aaggcctttt cttcaaggac ttgaagccct gcaatggttg
     2041 tccctccagg agatgaaacc atggctgtta aagcttgagg ttctttaccc gattgtttta
     2101 gtaattcaag actaccttct agagtagcta aagcaagctc ttttgatgcg gaacgagtga
     2161 gtcccatttt gactccaccc ataattaaac cttcaataaa ttcaaaaacg tacgcagggc
     2221 cgctgccaga taaacctgtc actgcatcca ttaaagattc ttgaacgcga acggtaattc
     2281 ccacattctt gaaaatggat tctgctaaaa caagtgtttc ttccgataca aaatctgcag
     2341 aaagagcgat agcccctttt ccaacagtta atggtaaatt aggcatgact cttaacactt
     2401 cgtgagaacc aagttcattt ttaagagtgg tagaggggat ccctgccata atagaaatga
     2461 atactttttt tgacacttgt tgaaggtctt ttttccaaga agaaaccacc gtattaaaaa
     2521 tctgaggttt aacacaaata aaagcggttt cagcttcatt aatcccttcc gcaaatgaag
     2581 aaacgcgaag agccccaaac gattcatatt ctttagcagt taaatcgttt ggttcaaaaa
     2641 ataaaatatc ttgtggagac ttttttgctt gaagcaatcc ttttagaatc gctccaccca
     2701 tattaccaac accgataaaa aatatttttt gattcatttt catttcctca catttatccg
     2761 ttcatagaag ataagaagtc tttatttgta atggcattga gcattttctc tcgcatgaat
     2821 tccataattt cagtagatgt atggtcttgc aagtagcgac gtagaatcca aacacgacgc
     2881 agttcatctt cggataagag aagttcttct tttcttgtac cagatttgaa tatatcaata
     2941 gctggccaaa ttctcttttc tgaaatacga cgatcaagaa cgagttccat attgccagta
     3001 cccttaaact cttcaaaaat cacttcatcc attcggctgc ccgtttcaat aagagctgtt
     3061 ccaataatgg ttagtgaacc gccattttca attttacgag cggcaccaaa gaatcttttt
     3121 gggcgttgca aagccatggc gtcgacacca ccagataaga ttttaccaga atgaggaatg
     3181 actacgttgt tggcgcgtgc aaatcgagta atagagtcta ataaaatgac aacatcatta
     3241 ccatgctcga cgagtcgctt tgctttttca agaaccatgt cagcgacttg aacgtgacgt
     3301 tctggaggtt cgtcaaatgt agaagcaaga acttcacctt tcacatgacg acgcatatcg
     3361 gttacttctt ctggacgttc atcaatcaat aatacaagaa gcttcacttc tggatggttt
     3421 gctgcgatgg cgtttgccac attttgaagt aaaaccgttt taccagtacg tggaggagca
     3481 agtaaaatac ctctttggcc tttaccaatt ggggtaaata aatccataat acgtgtactt
     3541 aactcttctt tattccattc cagattaaac ttttgaaaag gatgaatagg agttaaatac
     3601 tcaaaggaaa cacgacgctt tgccttttct ggagggtcaa aattaatcgt atcaattctc
     3661 attaaggcaa aatacttttc attatctcga ggctgtctaa cctgcccagt aatagaatcg
     3721 cctgttttaa gctcgaaacg tttaatctgg ttagggctaa tataaatatc gtcaggacca
     3781 gctaaatagt tatgatcagg gcttcttaaa aagccgtagc cttcgtttaa aatttcaaga
     3841 acaccttctg tatagatagg cacatcttcg cctgcggtgg cgccaagaat tctataaatt
     3901 aaagcttgtt tgcgtaaacg ttttacatct ggaatttcta attcaagagc ttgacgttga
     3961 agttcaaaca tcggcatgtt gcggagttgt ttccactttt gtacagcaag cgctactttt
     4021 tctggatcag ctggtggagc ttgaatcatt tcatctgtca catcatcttg aggcatgtag
     4081 cgattattgc gacgattttt atacttattg aatttattga acttattgtg tctttgattt
     4141 tgattagggt agcgttgtgg gtaatcgttc tgctgaggat ttgtttgact tggaacggct
     4201 tcagatgatt gagcttcttg ttctgctgag acaggagtct cattattttc aggtgaagaa
     4261 tattcttctt ctttgacata tgattcaggt gcagaaagat cttctatcac ttcctctgat
     4321 ttagattccg aaggctcttc agaaggtttt tcttttttag aacgagtaga ctttcttcct
     4381 ttattcttct ttgcggtttc ttcgggaaat tctattggtt gagctaaatc cccaagagct
     4441 tctaggttag gtatgggggc atctaatgga attgtatcag aaataagttt ggaatcgtct
     4501 gtagtagagt tctttttcgg tctcggcacg tatgcctcct aaaatagcaa aggatattgg
     4561 atatagtttc aaagataaaa tgatgaatcc cagtcaaata tttttataaa aaataaataa
     4621 acagcctttt tgactgaaac tgaataataa gatagataat ttccgtgttt ttgtcataaa
     4681 aaaggtgttt gaaaatctat atttggggtg tggaaacttt agaacgagta tttatagctt
     4741 taggtagcaa tatttctcca agaggacctc gattaaaggc agctcgtcaa atgcttcagg
     4801 gcatcgctgc tggaggctgg aaagaaagtg ctatctatga aacccctccg gttggacctg
     4861 aggggcagga taattatttc aatcaagtgg taagtttttg gtattccaag gggaaaggtc
     4921 gtcttttaaa ttacttaaaa ggagctgagc ttctcttagg ccgaaagcca agaggtcatt
     4981 ggaactcaag agaaatcgat ttagacttgt tgtattacgg ggagatcgtt tcttcaggtc
     5041 gtccaacact acctcatcca gaagttcaaa aacgtcaatt tgttttaatc cccctttgtg
     5101 atatcgctcc cgaatggaag gatccattaa caggtcgtac tcttcaagaa atactttttg
     5161 aattacgtcg tcgagaaggt gatcttggtt ttaagattgt tgatccggag gattcttaaa
     5221 tgatattaaa tccagatatt cgtttcttgg ctattgaagg cgtaattggt gtaggtaaaa
     5281 cttcactagc gaaagtgctt gcagagcgtt tgaatgcaac tctcttagaa gaaatgtttg
     5341 aagaaaatcc atttcttgaa aagttttatc aacaacctga agaatacgct tttcaaactc
     5401 agttattctt tcttttatca agacatcgtc aattactaga ttcatttatt caaaatgact
     5461 tgtttcgtca tttgattatt agtgattatt cttttgataa agatcgaatt tttgcccttc
     5521 aaaatctttc agaagatgaa atgtatatgt atgatgcggt tttaaaatca ttagataata
     5581 atgtgcctcg tccagattat atcatttatc ttcaagcaag tgttgaaact ttgattacta
     5641 gaattaaggg tcgtaatcgt cctatggaaa aagggattga cggtcattat ttgcgtgacc
     5701 tacaagaacg ttacgatcat catttttggc attataatga ttgtcctgta ttaatcatta
     5761 atacagataa ggtagatttt gtaaataatg aaaatcactt aaaacaaata ttaaatgtca
     5821 ttcaaacttg gccatcacaa acaacttatt ttattcctga aggttgatcc ccatgaaagt
     5881 cataaaaaca atcgcgtctc ttagacgcac tttatctaca tataacaaag ctggaaagaa
     5941 aattggctta gtgcccacta tgggagcact acatgaaggc catatgagcc ttgtggataa
     6001 aagcgtagaa gaaatggacg ttaccattgt tagtgttttt gtaaatccaa tacagtttgg
     6061 taaaaatgaa gatttagata aatacccaag gcgattagaa gcagatgcct ctttagcatc
     6121 tgagcatggg gctgatttta tctttgctcc ttctgttgct gaaatgtatc catctggtaa
     6181 ttcttctact ttaattagaa atgaggagtt agaaggcctt tattgtggcg cttatcgtcc
     6241 agggcatttc agaggtgttt taacagtggt agctaaactc tttatgatta cccaggcaga
     6301 taaggcttat tttggtttaa aagattacca acaagtcttt ttgatagaaa agatggttga
     6361 ggatcttaat attaaaacaa aaatagtccg tattccaata gtaagaggcc cctcaggctt
     6421 ggctctttcc agccgtaatg aatatcttac agatgaagaa aaagcgaatg cggtcgcttt
     6481 atttgaaggt ttaacagcag ccaaaaaagc atatagtgaa ggagaacgta gtgttgctaa
     6541 acttcgtgat tctgtgattc gctatatatt aatgaaaagg ggcg
//
LOCUS       DAOOBB010000107         6560 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_13833_length_6560_cov_5.043198, whole genome shotgun sequence.
ACCESSION   DAOOBB010000107 DAOOBB010000000
VERSION     DAOOBB010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 6560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6560
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13833_length_6560_cov_5.043198"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..802
                     /locus_tag="PLT31_05900"
     CDS             14..802
                     /locus_tag="PLT31_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94706.1"
                     /translation="MHLNSKTLFSGKNQAQKAFLHYGVFIAVIFLLLSCSSEKKEESS
                     ASQKKNAHKKIVVDESSRLLLDSMISSYMIIPWNETISKTIVLKQKETLDTIEIENSV
                     LIEGSFHGRKAYVFKNGPDCLGSFVTDSSGTLQFFNSSLFCLDSAVVNRELMRILWNT
                     KGVPAIFVSESYQERGSAIRIRRLDRDSGEVFIDEKVSNADICGPEEDEGVIPCFEWS
                     TTLLIDDSSNVLPDTLKIKRLGTVLENKRVLPMNQTDSVIINWQ"
     gene            989..1891
                     /locus_tag="PLT31_05905"
     CDS             989..1891
                     /locus_tag="PLT31_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPW94707.1"
                     /translation="MNTQLKKLITISLGLALVSLFVACDGQNKKVESKKEGAAFESKR
                     EIFVVTREQGSGTRDAFVELTGILVKVDGKKKDNTSKEALTIDGTQAVMSNIAGNEYA
                     IGYISLGSLNSSVKAISVNGNPISAETVKNGTYPISRPFNIATKGTVSAVAEDFIAFI
                     LSDEGQAVVEKNGYIAVQSGTKYAGKKPSGKIVIAGSSSVSPVMEKLKEAYLAINSNA
                     QIEIQTNDSSAGMVAAKEGTCDIGMASRALKDSEKEVLQSTVIAMDGIAVIVNNKNPL
                     KSLSMSQIREVFTGFIRNWEAVIE"
     gene            1884..2165
                     /locus_tag="PLT31_05910"
     CDS             1884..2165
                     /locus_tag="PLT31_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94708.1"
                     /translation="MNNLIAKYKEKIMEGVFFGTALFSIFAVILICIFLFAGGVPAIA
                     KIGAFSFLFGSEWAPTDDPASYGIFPMILGSLYVTAGAILIGVSIGVLL"
     gene            2149..3735
                     /locus_tag="PLT31_05915"
     CDS             2149..3735
                     /locus_tag="PLT31_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW94709.1"
                     /translation="MAFYYRMEDQVKEYLQEESRVITQVYSYLETPESLAPFASDIIR
                     ITLIDSLGHVLFESEKESAEMGNHLNRSEIQSALKTGSGESFRFSSTLETDVYYFAQK
                     LPDGHVLRLSMRKTNLFSIFSSVYPYLILLFVAIVLLSIISAFFLTKLFLKPIHEMIS
                     RLDSEHVVDDDTLIYHELVPFIVEINKQRKNTQHELKRLANEKEKLSVLMKNMPEGLL
                     FLDEHKNVVMINEEARSLLHVQEEHELRHIIYYSRNLHLNNLIDKAFLGKRISEEISL
                     NGMLYQVLASPVIVLNEFVGIICLFMDLSAKNKVEQLRREFTANASHELKTPLTSILG
                     YAEMIENNMVKTEDVPRIASKIHNESKRMLMLTEDIMKLAELDEVRFEEVAKDSVDLL
                     AVAQECASSLEPIAASKKIVMEVKGIATKIQGNRRLLFEMVYNLMDNAIRYNREGGSV
                     LVLVDEKSISVRDTGIGIPEKSQERIFERFYRVDKSRSKETGGTGLGLAIVKHVAESH
                     YAKVILDSKENKGTEIRIQF"
     gene            complement(3738..5273)
                     /locus_tag="PLT31_05920"
     CDS             complement(3738..5273)
                     /locus_tag="PLT31_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94710.1"
                     /translation="MSLLSLNRPAYAETHFKFKLPWSLLYRKRPEIIVDVPSQINANQ
                     SPELWIIIRDANRFPLQIKSIELKIKNEKQKIISIKKDLNIFANEPFSFYSIVLDPLP
                     AGTYEITPSVETIQKNHSKKWIRWNYPFLKPLPLKIQVLKEPYFIPSGFVAGEMHCHT
                     HYSSDHVEFGAAPHVLQSAARLIGLDFVLCTDHAYDFAFSKEDYTLPDQADVRFEALR
                     QEIQELPSSPLMIAGEEVSVGNSHNENVHMLCLSPREYLEGKGDCGRYGLNNKPTWGI
                     SKTISIANAPCFAAHPKVPMSKLERFVFRRGDWHDEDLQLESETPIRGIQFWNGSRDR
                     GFELGRLWWISLLEKGHEILPIGGNDAHGDLNDSVSITMPLISLRHNRNHVFGKVKTV
                     VKVNDSNALTIDSLKEGFQGDSLYLTDGPALWWSRENDFIHFQMNCSADFGTIQYLHF
                     YTAEKGDHSPEKLHPTYSVFQTSSQTLSVKIPLKDFRYVRAESETSKGHFALTSAAFA
                     KEI"
     gene            complement(5270..6337)
                     /locus_tag="PLT31_05925"
     CDS             complement(5270..6337)
                     /locus_tag="PLT31_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94711.1"
                     /translation="MVAFSKNPLSKNQPRYSRSVRPLKTFFFNTSFLEIVFILLFFSI
                     SLSFAQETKATSTISNAPTSAVSNDSMSVVSNDSTTVISNDSESVVSNDSSSVISKAS
                     TSSAASSTASADFKKAASEYADNSRFSFFIQPGISFLGFDDREKFQSAIDSIYLDYKA
                     EAVNNAESLAVVKQDFQKVNFTFPISGGIQFQHYKDHFISSGIGFIYNRESVVLTNRN
                     NKTFNYYYTLQAVPLFLEYRMTIPAKFITLTDKSLFSVAFRWYWMLPGTEIYSTWGKI
                     EAERSIAGNGFGLSLGYLIASWNSIRIYGDLGLTSISVSSKEPFSSIVPDASTEKAKW
                     DLGGIQLQIRFSWGAILIKES"
     gene            complement(6337..>6560)
                     /locus_tag="PLT31_05930"
     CDS             complement(6337..>6560)
                     /locus_tag="PLT31_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94712.1"
                     /translation="LMVKSYSDLDQFDMARLYLKEVLNDTSIDAKIKEKEVASLEKHI
                     AKAEVKYEEKLKKEHSQKQVKKIEAEDFN"
BASE COUNT         2134 a         1071 c         1312 g         2043 t
ORIGIN      
        1 ttaaaaggtt tttatgcatc taaatagcaa aactttattt tctgggaaaa atcaagctca
       61 aaaagcattt ttgcattatg gtgtttttat tgccgttatc ttcttacttc tttcttgtag
      121 ttcagaaaag aaagaagaat cttctgcttc ccaaaaaaag aatgctcaca agaaaatagt
      181 ggttgatgaa tctagtcgtc ttttattaga ttccatgatt tcttcgtaca tgattattcc
      241 atggaatgag acgatttcaa aaacgattgt tttaaaacaa aaagaaacat tagatacgat
      301 tgaaatagaa aattcagtgc ttatagaagg ctcgtttcat gggcgaaaag cctatgtttt
      361 caaaaatggt cctgattgtt taggctcttt tgttaccgat tctagcggga ctttgcagtt
      421 ttttaattct tcactttttt gcctagattc agctgttgta aatagagaat taatgcgtat
      481 tttatggaac acaaaaggcg taccagccat ttttgtctct gagtcttatc aagagagggg
      541 aagtgccatt cgtattcgac gactagatcg agattcagga gaagtcttta ttgatgaaaa
      601 agtaagcaat gcggatattt gtgggccaga agaagatgaa ggcgtgattc cttgttttga
      661 gtggtccacg actttattaa ttgatgatag ttctaatgtt cttccagaca cgttaaaaat
      721 aaagcgattg ggaacggttt tggaaaataa gcgtgtttta ccaatgaatc aaacggattc
      781 ggtgattata aactggcagt agtattattg ctaaaattcg caaatattag tagtaaatta
      841 cgttactttt gttggattga attaaataag ctttttttag gctaattttc gataatcact
      901 ttactttgat tttacatttt cctttcggtt cattttacat agaggctcta tcttatggat
      961 attaaacaat tcataggagt agagtataat gaatacacaa ttaaaaaaac tgatcacgat
     1021 atcacttggc cttgctttgg tgagcctatt tgtggcgtgt gatggacaaa ataaaaaagt
     1081 agaatcaaaa aaagaaggtg ctgcttttga atcaaagcga gaaatatttg tagtgactcg
     1141 agaacagggg tctggaactc gcgatgcctt tgttgaatta acaggcattt tagttaaagt
     1201 agatgggaaa aagaaagata atacctctaa agaggctttg acgattgatg ggactcaagc
     1261 ggtcatgagt aatatagcag gtaatgaata tgcgattggt tatatttctc ttggatcctt
     1321 gaactcttcg gtgaaagcta tttctgtcaa tggaaacccc ataagtgcag aaacagtgaa
     1381 gaatggtact tatccaattt ctcgcccatt taatattgcc acaaaaggaa cagtgagcgc
     1441 cgttgcagaa gattttatag cctttatttt aagtgatgaa gggcaagcgg tagtagaaaa
     1501 gaatggttat attgctgttc aatctggaac aaaatatgca ggaaaaaagc cttcaggtaa
     1561 aattgtaatt gctggttctt cttcagtatc tccagtgatg gaaaaattaa aagaagctta
     1621 tttggcaatc aatagtaatg ctcagataga aatccaaacg aatgattcct ctgcaggtat
     1681 ggttgctgct aaagaaggca cttgtgatat agggatggct tcaagggctc taaaagatag
     1741 tgaaaaagaa gttcttcaaa gtacagtgat tgcaatggat ggaatagccg ttatcgttaa
     1801 taataaaaat ccattaaaat cgctttctat gtcacaaatt cgtgaagtat tcactggatt
     1861 catacgcaat tgggaggcgg tcattgaata atctgatcgc aaaatataaa gagaaaataa
     1921 tggaaggtgt ctttttcggc accgctctat tctctatctt tgcagtcatt ttaatatgta
     1981 ttttcctctt tgcaggaggc gttcctgcca ttgcgaaaat aggtgccttt tctttcttgt
     2041 ttggctcaga atgggctcca acggatgatc ctgcttctta tggaattttc ccaatgattt
     2101 tgggaagttt atatgttact gcgggagcca tattaattgg ggtttcgatt ggcgttttat
     2161 tatagaatgg aagatcaagt taaagaatac ttgcaagaag aatcgcgagt tattactcaa
     2221 gtatactctt atttagaaac gccagaaagt cttgctcctt ttgcctctga tattattcga
     2281 attacgctga ttgattcctt aggtcatgtt ctttttgaaa gtgaaaagga atcagcagaa
     2341 atgggaaacc atttaaatcg ctcagaaatt caaagtgctt taaaaacagg ctctggtgaa
     2401 agttttagat tttccagtac attagaaacg gatgtgtatt attttgcaca gaagcttccc
     2461 gatggtcatg tattaagact ctccatgaga aaaacaaatt tattctctat tttttcttct
     2521 gtatatccat acttaattct gctttttgtc gccattgtat tattgtctat tatttctgct
     2581 ttttttctca caaagctttt tttaaaacca attcacgaaa tgatttctcg tttagattca
     2641 gaacatgtcg ttgacgatga tacccttatt tatcatgaac tggttccttt tattgttgaa
     2701 ataaataaac agcgtaaaaa tacgcaacat gaattaaagc gtcttgctaa tgaaaaagaa
     2761 aaactatctg ttttgatgaa gaacatgccc gaaggattat tgtttctaga tgaacataaa
     2821 aatgtagtga tgattaatga agaagcaagg tcattactcc atgttcaaga agaacacgaa
     2881 cttcgtcata taatttatta ttctcgtaac ttgcatttaa acaaccttat tgataaggct
     2941 ttcttaggta agcgcatttc agaagaaatc tctttaaatg gaatgctata tcaagtgctt
     3001 gcaagtccag tgattgtgtt aaacgaattt gtgggtatca tttgtctttt tatggacttg
     3061 tctgcaaaaa ataaagtaga acaattgcgc cgagaattta cagcgaatgc ttctcatgaa
     3121 ttaaaaacac cattgacttc tattttaggt tatgctgaga tgattgaaaa taatatggta
     3181 aaaacagaag acgttcctcg tattgcaagt aaaattcata acgaatccaa gcgaatgctt
     3241 atgcttacag aagatataat gaagttagca gaattagatg aagttagatt tgaagaagta
     3301 gccaaagatt ctgtagattt attagcagtg gcacaagaat gtgcttcttc attagagcca
     3361 atagcagctt caaaaaagat agtgatggaa gtaaaaggta ttgcaacaaa aatacaaggc
     3421 aatcggcgct tactttttga aatggtttat aatttaatgg ataatgcgat tcgttataat
     3481 cgtgaaggcg ggtcagtatt agttcttgtc gatgaaaaaa gtatttctgt gcgagatact
     3541 ggaattggta ttccagaaaa aagtcaggaa cgaatctttg aacgctttta tcgtgtagat
     3601 aaaagtcgat caaaagaaac gggtggaaca gggctaggcc tagctattgt aaaacatgtt
     3661 gccgagtccc attacgcaaa agtgatttta gattctaaag aaaataaagg aacagaaatt
     3721 cgaatacaat tttagattca gatttcttta gcaaatgcag cgcttgtgag agcaaaatgg
     3781 cctttagagg tttcactttc ggcacgaaca tagcgaaaat cttttagagg aatcttaaca
     3841 gaaagtgttt ggctactggt ttgaaaaaca gagtacgtcg ggtggagctt ctcaggcgaa
     3901 tggtcgcctt tttctgccgt atagaaatgt aaatattgaa tagtcccaaa atcagcagaa
     3961 caattcattt gaaaatgaat aaaatcattt tcacgagacc accaaagggc agggccatca
     4021 gtcaagtaca gagagtctcc ctgaaagcct tcttttagag aatcaattgt taaggcatta
     4081 gaatcgttga ctttcactac cgtttttact ttcccaaaaa catggttcct attatgacgt
     4141 agagaaatga gaggcatagt aatacttacc gaatcattta aatcaccatg agcgtcgttt
     4201 ccgccaatgg ggagaatctc gtgccctttt tcaagcagag aaatccacca taaacggcct
     4261 aattcaaacc cgcgatctct tgacccattc caaaactgaa tgcctcgtat aggagtttca
     4321 ctttcgagtt gtagatcctc atcatgccag tccccgcgtc taaaaacaaa acgttcgagc
     4381 ttgctcatcg gcaccttagg gtgtgcggca aagcacggag cattagcgat agaaatcgtt
     4441 tttgaaatac cccaagtagg cttgttattt aagccataac gcccgcaatc gcctttccct
     4501 tctaaatact ctcttggaga aagacaaagc atgtggacgt tttcattatg agagttcccc
     4561 acagatactt cttctccagc aatcatcaaa ggagagcttg gtaattcctg aatttcttgt
     4621 cgaagtgctt caaaccgaac gtctgcctga tcgggcaacg tatagtcttc ttttgaaaaa
     4681 gcaaaatcat aagcatggtc tgtgcaaaga acaaagtcta atccaatcaa gcgggccgcc
     4741 gattgaagca catggggcgc cgctccaaat tccacgtgat ccgaagaata gtgtgtgtgg
     4801 caatgcattt cgccagcgac aaaaccagaa ggaataaaat agggctcttt taatacttga
     4861 atttttagag ggagaggctt taaaaaaggg taattccaac gaatccattt cttagaatga
     4921 tttttctgaa tcgtctccac agaaggagtg atttcataag tcccagcagg tagaggatct
     4981 aagacgatag aataaaaaga aaaaggttcg tttgcaaaaa tatttaagtc tttttttata
     5041 gaaataatct tttgcttttc attttttatt ttcagttcga tggattttat ttgcaaagga
     5101 aatcgattgg catctcgtat aataatccat aattcgggag attgattcgc attgatttga
     5161 gatggaacat ctacaataat ctctggccgt tttcgataaa gtaaagacca aggcagctta
     5221 aatttaaaat gcgtttctgc gtaagcaggg cgatttaaag aaaggaggct catgactctt
     5281 ttataagaat cgctccccaa gaaaagcgta tttgtaattg aatcccaccg agatcccatt
     5341 tggctttttc agtagaagcg tctggaacaa tagatgaaaa aggttcttta gaggaaacag
     5401 agatagaagt taggcctaaa tcaccatata ttcggataga gttccaagag gcgattaaat
     5461 agccaagact taatccaaag ccatttccag caatggagcg ctctgcttct attttccccc
     5521 aagtggaata aatctcagtg cctgggagca tccaatacca tcgaaaagca acactaaaaa
     5581 gagacttatc tgttagggtg ataaacttag caggaatggt cattcggtat tctaaaaata
     5641 aaggaacggc ttgtagtgta tagtaatagt taaacgtttt attatttcga ttggtaagaa
     5701 caacggattc tcgattataa ataaagccta ttccagagct aataaaatga tctttatagt
     5761 gttgaaattg aataccgcca gaaataggaa atgtaaaatt taccttttga aagtcttgtt
     5821 ttacaacggc gagtgattcg gcattattga cggcttctgc tttataatca agataaatcg
     5881 aatcaattgc tgattggaat ttttcacgat cgtcaaagcc taaaaaagaa atccctggtt
     5941 ggataaaaaa ggaaaatctt gaattatcgg catattcaga agccgctttt ttaaaatcgg
     6001 cagaagctgt ggaactggcc gcacttgatg tcgatgcttt tgaaataaca gacgaagaat
     6061 cgtttgaaac aacagactct gaatcgtttg aaataacagt tgtagaatca tttgaaacaa
     6121 cagacatcga atcgtttgaa accgcagaag tcggcgcatt tgaaatggtg gatgtcgctt
     6181 ttgtttcttg agcaaaagat aaggaaatag aaaaaaacaa aaggatgaag acgatttcta
     6241 aaaaagaagt attgaaaaaa aatgttttta aaggacgaac cgatctggag tatcgtggtt
     6301 gatttttgga taatggattt ttagaaaaag caaccattag ttaaaatctt cggcctctat
     6361 ttttttcact tgtttttgag aatgttcttt tttcaatttt tcttcatatt taacttcggc
     6421 tttcgcgata tgcttttcaa gagaggccac ttctttctct ttaatcttgg catctataga
     6481 ggtatcgttc aagacttctt ttaagtagag gcgagccatg tcaaattgat ctaaatcgga
     6541 atagctcttt accattaaaa
//
LOCUS       DAOOBB010000108         6549 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_13856_length_6549_cov_4.985063, whole genome shotgun sequence.
ACCESSION   DAOOBB010000108 DAOOBB010000000
VERSION     DAOOBB010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 6549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6549
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13856_length_6549_cov_4.985063"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="PLT31_05935"
     CDS             <1..344
                     /locus_tag="PLT31_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94713.1"
                     /translation="KYENNQWTELCWSTEDKQKTYEHEVQHYRNAKKAIEYFYNRHLL
                     TKKNNTATNNLFTQKECIDAGNDLIKKIVDYYNQWEENEKQHRYPGYKEHIPPSPIPT
                     FQNRVPIPCPN"
     gene            332..832
                     /locus_tag="PLT31_05940"
     CDS             332..832
                     /locus_tag="PLT31_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94714.1"
                     /translation="MPKLIILLLLLLIQGNTSESSSSVKIEPTDKWTVIGKETIMVQG
                     KPIELTVWVKKEFYNGEAYFIYRKENEEKSIMIDSTVDLDGTYSVWENNYITRISRRR
                     ERTGSESYRFFDESGKIIKKYYIGLPKNEIYDENGWLMPDLKEVRGDTAFVDLEYYDA
                     ITIPVD"
     gene            842..1063
                     /locus_tag="PLT31_05945"
     CDS             842..1063
                     /locus_tag="PLT31_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94715.1"
                     /translation="MPKLIILLLLLLIQGNTSESSSSVKIEPTDKWTVIGKETIMVQG
                     KPIELTVWVKKKNQLFLLFNRDIFNNRNL"
     gene            1089..1871
                     /locus_tag="PLT31_05950"
     CDS             1089..1871
                     /locus_tag="PLT31_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW94716.1"
                     /translation="MMELNNKKPILSIQHLHRNFQMGEETVHALRGVSYDIYPGEFVT
                     IMGASGSGKTTMLNILGCMDRPSSGHYILDNEPVENLKRDALARIRSKKIGFVFQSYN
                     LLSRTTAIENVELPLLYNSEISSEERRARAVAALELVGLAGRMDHMPNQLSGGQQQRV
                     AIARALVNDPVIILADEATGNLDTRTSYEIMMIFQNLNKQGKTIAFVTHEPDIAAFSS
                     RTITLRDGLLKKDYYNNHVLSAEQALKDLPPSDLELSDGGDE"
     gene            1868..3082
                     /locus_tag="PLT31_05955"
     CDS             1868..3082
                     /locus_tag="PLT31_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW94717.1"
                     /translation="MSPITLIKIALRALLRNRMRTFLSVLGIVIGVAAVITMVAMGEG
                     SKKSIRDQMTSMGTNAITIMPNSSRRGPVQLDASGSTETLEEADIFALRKKAVYIKEV
                     SPVVTANGQAVVGNNNWPVSLTGISAEYLKIRNYEMEDGIMFDDQIDRMAKVCVIGQT
                     VVNNLFPEGDPIGKTIRYKNIPLKVIGTLKPKGSSGFGQDQDDVVFTPYQTVMKRFNA
                     NTEIRTIYANSIGEGYAKQATNEVLEILKERRNWTKPTEPFRVRTQEEMIQMVTSTSE
                     LLSIVLTAIAGISLFVGGIGIMNIMYVSVTERTKEIGLRMAIGARGKDILLQFLFESV
                     IISLLGGLVGILLGILSAQGVKLFLNWPMSVSFTSVIVSFLVCFATGVFFGWYPAKKA
                     SRLDPIEALRFE"
     gene            3204..3833
                     /locus_tag="PLT31_05960"
     CDS             3204..3833
                     /locus_tag="PLT31_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HPW94718.1"
                     /translation="MLSILLKSAPVIGILRDIPMGAENDCLRVSKRCGLKAIEVTMNT
                     PNAEVIIKNMRSIADPLDILVGAGTVRYLSELETAISAGAQFIVTPTISEEIIKRCVN
                     LQIPIIPGALSPTEIQRAYDLGASFVKVFPVGAMGGASYIRDLRGPFRDIPLMACGGV
                     SEKNAPEYRESGADLLAFGGSIFNPTQMRLGQWDLIEEKLSTFMARACL"
     gene            complement(3842..5356)
                     /locus_tag="PLT31_05965"
     CDS             complement(3842..5356)
                     /locus_tag="PLT31_05965"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HPW94719.1"
                     /translation="MRIMTLFKKSSSQNLIPYNGNKAFLKKTLILSFAILNFCILGCT
                     VDSAGVITETESGKTLAGIVTDSKGIPLANVQVALIDTQFISARPLFKREVISDDSGY
                     FIIDSIPQGSYRLALMDSSKDQSLLTSVDLSKEDSLQTISVNNLKLLKNGSFNLDLNL
                     FQLSAGDTLCLTGSLFCKVIENGDDSLRHIEVTGMTPILFRSLHILKANHSSEQVDIQ
                     WNIESEKKLFITEHGNFFSKTTISKVFEDSLMNGGYTGDSIPMRFVLSNTWEKPMLLD
                     TNGNTILLQPISTQDDSIVYFGIVPKVDFSKSTTLSFAVIDSAQNGSLNTPPVRSFHY
                     FNESLTNSTTITEPVFQDSAIGLSFRFKIDGKDQDTLGVVLISARTAEGKGFDIRQRE
                     STDSVSICVRLYSDTLNNVIAASDTIIYGAAKLLDNEWHHYTLFINKAHLTISADGEI
                     IRNTDIKVTENFYNPSEIIIGSDPLFRGSLTELFILDGKQDSSWIKTQYELQKR"
     gene            complement(5353..6189)
                     /locus_tag="PLT31_05970"
     CDS             complement(5353..6189)
                     /locus_tag="PLT31_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02147 family protein"
                     /protein_id="HPW94720.1"
                     /translation="MNKETNIFQFTHFRKYLEQYQEQRVLEEPSFTRTEICNLLGLSK
                     SRSYFADVLRGKKVSPRMVQKFIEILNLNKKEAQYFKAMVQLDQAKNEQVRSQAMEEL
                     LHIHPTPEHLLNSDAYDYYAKWYHSALFAILDVLDINDDLRPVQKRIFPKVSLGKLTS
                     SIQLLIRLGLVRQNDDGFYKPTKDSISSGPYNNDELIRQYQLQCFELSKEALLTPSKN
                     PHIMSTLVFSLSKEAYQKMEAELQAFKKAARRIVAEDSNKPDSVYHLNLHLFSNLEPE
                     GK"
     gene            6321..>6549
                     /locus_tag="PLT31_05975"
     CDS             6321..>6549
                     /locus_tag="PLT31_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94721.1"
                     /translation="MKSFSFFHVTLLLCLFFISSVYAIRMDLGVGLGLGYSGLITSDS
                     KSNDLEDYNGGSASQFSVYGIYFFKENMAWYF"
BASE COUNT         2121 a          960 c         1397 g         2071 t
ORIGIN      
        1 ggaaatacga gaataaccaa tggacagagc tttgttggag tactgaggat aagcaaaaaa
       61 cttatgaaca tgaagtacaa cactatagga atgcaaaaaa agcaatagag tatttttata
      121 atagacattt gttgactaaa aaaaataata cagcgactaa taatttgttt acacaaaaag
      181 aatgtattga tgcgggaaat gatttgataa aaaaaatagt cgattattat aatcaatggg
      241 aagaaaatga aaaacaacat cgttatcctg gctataaaga acatattccc ccaagcccta
      301 taccaacatt tcaaaataga gtgccaatac catgcccaaa ttaataattt tactattact
      361 tttattgata caagggaaca cttctgaaag ttcaagtagt gttaaaatag aacctaccga
      421 taaatggact gtaatcggaa aagaaactat aatggtgcaa ggtaaaccaa tagaactaac
      481 ggtttgggtg aagaaggagt tttataatgg tgaggcctat ttcatatacc gtaaagaaaa
      541 cgaagaaaaa tcgattatga ttgattctac agtggattta gatgggacat atagtgtttg
      601 ggaaaataat tatataacac gaatttcaag aagaagggaa cgaacaggat ccgaaagtta
      661 taggtttttt gatgaatcag gtaaaatcat aaaaaagtat tatatcggtt tgccaaaaaa
      721 tgaaatttat gatgaaaatg gttggttaat gccagatttg aaagaagtta gaggagatac
      781 agcttttgtt gaccttgagt attatgacgc cattacaata cctgtagact aatactttac
      841 catgcccaaa ttaataattt tactattact tttattgata caagggaaca cttctgaaag
      901 ttcaagtagt gttaaaatag aacctaccga taaatggact gtaatcggaa aagaaactat
      961 aatggtgcaa ggtaaaccaa tagaactaac ggtctgggtg aagaagaaaa atcaactttt
     1021 tttgcttttt aatagagata tttttaataa taggaaccta tagccgcctt taatcatcta
     1081 attttcaaat gatggaacta aacaataaaa agccaattct ctctattcag catttgcatc
     1141 gaaattttca aatgggtgaa gaaacagttc atgctttacg cggtgtgagt tatgatattt
     1201 atcctggaga atttgtaaca attatgggag caagtggatc tggtaaaacc acaatgctta
     1261 atattttggg ttgtatggat cggcctagct ctggtcatta tattttagat aatgagcctg
     1321 tagaaaactt aaaaagagat gctttagcaa gaattagaag taaaaaaatt gggtttgtgt
     1381 ttcagagtta taatttattg tcaagaacta cagcgattga aaatgtggag cttcctttgc
     1441 tttataatag tgagattagt tcagaagaaa gaagagcgag ggcggtggcg gctcttgaat
     1501 tagttgggct tgcaggaaga atggatcata tgccaaatca gctttctgga ggtcagcagc
     1561 aacgagtagc aattgcgcgt gcgttagtga atgatcctgt gattattctt gccgatgaag
     1621 caacaggtaa tttagatact agaactagtt atgaaattat gatgattttt cagaatttga
     1681 ataagcaggg caaaacgatt gcttttgtca ctcatgaacc tgatatagct gctttttcta
     1741 gtagaacaat tactcttcga gatgggttat taaaaaaaga ctattataac aatcatgttt
     1801 tgagcgcaga acaagcgttg aaagatttac ctccttcaga tttagaactt agtgatggag
     1861 gtgatgaatg agtccgatta ctttgattaa aattgctttg agagcactat taagaaatcg
     1921 gatgcgtaca tttttgtctg tacttggtat cgtgattggt gttgctgccg tgattacaat
     1981 ggtcgctatg ggagaagggt ccaaaaaatc aattcgagat caaatgactt cgatggggac
     2041 aaatgcgatt accattatgc ctaattcttc acgaagaggg cctgtccaat tagatgcgag
     2101 tggctccact gaaacgttag aagaagccga tatttttgca ttgcgaaaaa aagccgttta
     2161 tataaaagaa gtttcacctg tggtgactgc aaatgggcaa gcagtagtgg ggaacaataa
     2221 ttggcctgtc tctttaactg gaattagtgc ggagtactta aaaatccgaa actatgaaat
     2281 ggaagatggt attatgtttg atgatcaaat agaccgaatg gcaaaagtct gtgtgattgg
     2341 gcaaaccgtg gtgaataatc tatttccaga aggagatccc ataggaaaaa cgattcgcta
     2401 taaaaacatt ccattaaaag taatcgggac attaaagcca aaaggttcca gtggttttgg
     2461 gcaagatcaa gatgatgtgg tttttactcc atatcaaaca gtcatgaaac gctttaacgc
     2521 gaatacggaa attcgtacga tatatgcgaa ttcgattgga gaagggtatg ctaaacaagc
     2581 tacaaatgag gtgttagaaa ttttaaaaga acgccgaaac tggacaaagc caacagaacc
     2641 atttagagtt agaactcaag aagagatgat tcaaatggtc accagtacgt ctgagttgct
     2701 ttcgattgtg cttacagcaa ttgcaggtat tagtcttttt gtgggaggca tagggattat
     2761 gaatattatg tatgtttctg tgacggagag aacgaaagag atagggcttc gtatggcgat
     2821 tggtgcgcgc ggtaaagata ttttacttca gtttttattt gagtctgtaa ttattagttt
     2881 gcttggtgga ttagtcggaa ttcttttggg tatattatca gctcaaggtg tcaaattatt
     2941 cttaaattgg cctatgagtg tttcgtttac aagtgtgatt gtgagttttc ttgtttgttt
     3001 tgcaacgggt gtattttttg gatggtaccc agctaagaaa gcgagtcgtt tagatcctat
     3061 tgaagcactt cgttttgaat aactttcaaa gaaatgaaat gtggttataa tcgtgttatt
     3121 gctaatttga gtgacgaaat atggcgttaa gtgtcctata agaaaggata taaaaaatag
     3181 ggtttttata aaataggtga attatgttaa gtattttgtt aaagtctgct cctgtgattg
     3241 gcattttaag agatattcct atgggagcag aaaatgattg cctacgagtt tcaaaacgtt
     3301 gtggcttaaa ggcgatagaa gtgacaatga atacgccaaa tgcagaggtg atcataaaaa
     3361 atatgcgctc cattgctgat ccattagaca ttttggttgg cgcgggtact gttcgatatt
     3421 taagcgaatt agaaacggct atttctgctg gtgctcaatt tattgtgacg ccaacgattt
     3481 cagaagaaat tatcaagcgc tgtgtaaatc ttcaaatacc tattattcct ggagcacttt
     3541 ctccaacaga gattcagcgt gcttatgatt taggcgcttc ttttgttaaa gtgttccctg
     3601 tgggggccat gggaggcgct tcttatattc gagatttaag aggcccattt agagatattc
     3661 cattaatggc ttgtggcggc gtgagtgaaa aaaatgctcc tgagtataga gagtcaggag
     3721 ctgatttgct tgcgtttgga gggagtattt ttaatcctac acaaatgcgt ttggggcagt
     3781 gggatttaat agaagaaaag ttatctactt ttatggcaag agcatgtctc taaaaatagt
     3841 tttaacgttt ctgtaattcg tattgtgttt taatccacga cgaatcttgt tttccatcca
     3901 aaataaaaag ttcggttaga gagcctctaa acaaggggtc gcttccaatt attatctcag
     3961 atgggttata aaagttctct gtcactttga tatcggtatt gcgtatgatt tcgccatctg
     4021 cactaattgt gagatgtgct ttattaataa aaagcgtgta atgatgccat tcgttatcaa
     4081 gtagttttgc tgctccataa atgatggtat cacttgccgc tataacgtta ttaagtgtat
     4141 cggaataaag cctgacgcaa atagacacag aatcggtgga ttctctttga cgaatgtcaa
     4201 aacctttccc ttctgcagta cgagccgaaa taagaacaac gcctaatgtg tcttgatctt
     4261 tgccgtctat cttgaatcgg aaagagagtc caatcgcgct atcttgaaaa acgggttctg
     4321 tgattgttgt actattagtg aggctttcat taaagtaatg aaaggatctt acaggaggcg
     4381 tatttaagga accattttga gcggaatcaa tgacagcaaa ggaaagcgtg gttgatttag
     4441 aaaaatctac tttaggaaca attccgaaat agacaataga atcgtcttgg gttgaaattg
     4501 gttgtaatag aatcgtgttt ccgttggtat cgagaagcat tggcttttcc caagtgtttg
     4561 acaatacaaa gcgcattgga attgaatcgc cagtgtagcc tccattcatt aatgagtctt
     4621 caaaaacttt tgagatcgtt gttttgctaa agaaatttcc atgctctgta ataaagagct
     4681 ttttctctga ttcgatattc cattgaatat caacttgttc agaagaatgg tttgctttta
     4741 aaatgtgtag gcttctaaat aagataggag tcattccagt gacttcaata tggcgaagag
     4801 aatcatcacc gttttcgatg actttacaga atagggagcc cgtgaggcaa agagtatcgc
     4861 ccgcgcttaa ttggaataga tttaagtcta agttgaaaga accatttttt aatagcttga
     4921 gattatttac tgaaatggtt tgaagagaat cttctttgct taaatcaaca gaagtgagta
     4981 gagattggtc tttagaggaa tccattaagg ctaaccgata agatccttgt gggatactat
     5041 caattataaa atagccagag tcatcagaaa tgacttctct tttaaataaa ggccttgcag
     5101 aaataaactg agtatcaatc aatgccactt gaacattagc taaaggaatc cctttagaat
     5161 ctgtgacgat acctgctagt gtttttccag attctgtttc agtgattact cctgcagaat
     5221 ctacggtaca cccaagaata caaaagttca gaatagcaaa agaaagaatt agagtttttt
     5281 tcaaaaacgc tttatttcca ttgtatggaa tgagattttg ggaggatgat tttttgaaga
     5341 gagtcatgat tctcattttc cctccggctc taaattagag aataggtgta aatttaggtg
     5401 gtagacgcta tcgggtttgt tcgaatcttc ggcgacaatg cggcgggctg ctttcttaaa
     5461 ggcttgcaat tcggcttcca ttttttgata agcttctttt gataagctaa agacaagcgt
     5521 ggacatgata tgaggatttt tggagggcgt taaaagcgcc tcttttgaaa gttcaaagca
     5581 ttgcaattgg tattgacgga tgagctcatc attattgtaa ggaccgctag aaatagaatc
     5641 ttttgtaggt ttgtaaaaac catcgtcatt ttgacggact aaaccaagac gaattaataa
     5701 ttgaatagat gaagtgagtt tccctaaaga gacttttgga aaaattcgct tttgaacagg
     5761 ccttaaatca tcattaatat ctaaaacatc taaaatggca aaaagcgcac tgtgatacca
     5821 tttggcgtaa taatcgtagg catcggaatt gagtaagtgt tctggcgttg ggtgaatatg
     5881 aagaagctct tccatcgctt gggagcgtac ttgttcattt tttgcttggt caagctgaac
     5941 cattgcctta aagtattgag cttctttttt atttaagttt aaaatttcta taaacttctg
     6001 aaccattcga ggagatactt ttttccctct taaaacatcc gcgaagtaac ttctgctttt
     6061 tgaaaggcca agtaaattgc atatttcagt tcgggtaaaa gaaggttctt caagaacacg
     6121 ctgttcttga tactgttcta aatatttacg gaaatgcgta aattgaaaaa tatttgtttc
     6181 tttattcacg ttctaaatat aaataataaa tcaatttctg agaacataaa tgagaacaaa
     6241 aattggaaaa tgaggacaat aatagataaa aaagaatata tatatagact aagtctactt
     6301 tttcattgta taggggtgta atgaagtctt tttctttttt tcatgtaaca cttctcttgt
     6361 gtttattctt tatttcttcg gtatatgcca taaggatgga tttgggagtc ggtttaggtc
     6421 tagggtattc gggattaatt accagtgatt ctaaaagtaa tgatctggaa gattataacg
     6481 gcggaagtgc gtctcaattt tctgtttatg gaatttattt ttttaaagaa aatatggctt
     6541 ggtatttta
//
LOCUS       DAOOBB010000109         6548 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_13858_length_6548_cov_4.236408, whole genome shotgun sequence.
ACCESSION   DAOOBB010000109 DAOOBB010000000
VERSION     DAOOBB010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 6548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6548
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13858_length_6548_cov_4.236408"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PLT31_05980"
     CDS             complement(<1..336)
                     /locus_tag="PLT31_05980"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPW94722.1"
                     /translation="MKKNLQKKGGIKDVLVVALPMLLSMSFDTIMTFADRLFLSRVGS
                     EYMNAALSGGITQMMILTFFTGTISYATALVAQNLGAERVENCAKVLTQAGIIACISA
                     PILLLIKPLA"
     gene            388..1746
                     /gene="ffh"
                     /locus_tag="PLT31_05985"
     CDS             388..1746
                     /gene="ffh"
                     /locus_tag="PLT31_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HPW94723.1"
                     /translation="MFSQLTDSLESTLKKLRGQGTLTEENVADALREVRRAFLEADVN
                     FNVTRDFVKAVKEKALGTDVLLSVTPGQQIVKIINDELIQMMGGETKELQLTGVPPTG
                     IMMVGLQGSGKTTFAGKIALYMRSKRKRKPLLVAADVYRPAAIKQLQVLGKSIGIPVY
                     EEGQGNPVEIIKNGHRYAIENGYDLVIYDTAGRLQIDDVLMQELEQARDAVHPEEILF
                     VADAMIGQEAVSVAETFFQRLNFTGVCLSKMDGDTRGGAALSIKKMTGVPICFVGLGE
                     KLSEIDLFHPDRMASRILGMGDVVSLVEKAQSVIDEKDAKDLKKKIFNNTFDLNDFLK
                     QLRTIKKLGRIKDIMGLIPGLNKLPLDQINEKELVYVEATLSSMTPRERKKPQIIDSS
                     RKKRIAQGSGTSVQKVNQVLSQYDSMKEMFKKIGGMAQKQNGGTPVGSNYTPPKVKKK
                     RK"
     gene            1920..2330
                     /gene="rpsP"
                     /locus_tag="PLT31_05990"
     CDS             1920..2330
                     /gene="rpsP"
                     /locus_tag="PLT31_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002852274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPW94724.1"
                     /translation="MATVIRLSRFGKRHHAVYRVVVVDNRKARDGQFIEQIGFYNPNQ
                     ATPDINFDQEKVLKWLSVGAQPSDTVASLLRNQGIMDLFHELKAKRSIENKAATPRAK
                     KAKKAPLGPKAKARLEAEKAAKEAPATEAPATEA"
     gene            2385..2939
                     /gene="rimM"
                     /locus_tag="PLT31_05995"
     CDS             2385..2939
                     /gene="rimM"
                     /locus_tag="PLT31_05995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HPW94725.1"
                     /translation="MEKDLLVIGQFMRPHGVQGYIKCKPETHDLNRYSRLTSLFVYQK
                     GTLLSLEIEDAKIQSGVWLFKFKGYDSPESIKPFTNLEACISNSERIPAPPGQIYFSD
                     AADFQVALEDGTIVGKVLEMIELPSVNAFCIRFNSPYNEQFTNQEILAPWIDACIIDI
                     NEKSKIIFCDELFLKSLCENGDSK"
     gene            2936..3640
                     /gene="trmD"
                     /locus_tag="PLT31_06000"
     CDS             2936..3640
                     /gene="trmD"
                     /locus_tag="PLT31_06000"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPW94726.1"
                     /translation="MKIQCITLFPEMFAPMDYSIIGRARQKKIFSFETIYLRDFAINE
                     YGQVDDSPYGGEPGMVLRPEPLTKAIRSTGVKNNGGKVVYLTADGVPFTHELALSLSK
                     EKQIVFVCGHYKGIDERVRESEIDLEISIGDFVVSGGELPAMVVTDAIVRLLDGSLGN
                     KESGETDSFAQGPLGWPVYTRPEEFEGKKVPEILLSGHHKKILEWQKAESLKRTQERR
                     PDIYAKLKIDTKFGIK"
     gene            3654..4001
                     /gene="rplS"
                     /locus_tag="PLT31_06005"
     CDS             3654..4001
                     /gene="rplS"
                     /locus_tag="PLT31_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HPW94727.1"
                     /translation="MSLNIEVIHQENKKTEQADFRAGDSVTVNVKVIEGTKERIQPFK
                     GVVIQKKNTGIATTLTVRKMSGNVFVERIFPVHSPRIDSIVVDRPGKVRQARIYYMRD
                     LRGKAARISERKV"
     gene            4014..4913
                     /locus_tag="PLT31_06010"
     CDS             4014..4913
                     /locus_tag="PLT31_06010"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPW94728.1"
                     /translation="MQKVIPPVQMCVKAGFLVFKPGESPRQLIIVDDGKLIAYELIDG
                     EKHIAFEMNPGDLIGVAALLEKEEVRYQIEAAVDSKITIIDEECMESQLIKTPVWLVA
                     LMKSLSLRTRELKKLKKQTGSLNTLQSLTLFLYYKNQKELPLLDTIREYCFQTRCAIK
                     TAYADLKRLQRKDIIYLKGDNEESILIREPLLLSILTDCFLCKQTQSPYYPFHLSSLE
                     KEGLIFLSKYSSSESLESSTWLQILQKQNPLFSASEWIRFQEYQIFVKDPENKYFIHQ
                     KKIQEIRLALENEKSIKKIILCN"
     gene            4904..5812
                     /locus_tag="PLT31_06015"
     CDS             4904..5812
                     /locus_tag="PLT31_06015"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPW94729.1"
                     /translation="MQLKAGDYLFMEGSFDDHLYIVKKGFLTAERREHGMTQVQWTFE
                     PGSLIGEGSLLENRPHTHAVKASVDSELIALDQNELKETLNKAPNWIVSIVYFLSRRF
                     REAEKKHSQNNMIRALPSLLFILFQSPQKKKYDVKEVVKDLFILTGLRSKEAFSLLKI
                     LESLGLFKVFDEQIQSTNTRVIQMLYEALLYRASKKEIPATILSITDQLILNAFIQTT
                     KKNGTIYKGQTAIDTAVFFKTGPRYAHGLRFSLKSLDDLLHKKILTHVAEGSSDFVYG
                     DIDIIVDLLELNRIYPLLDKKLIESP"
     gene            5924..6268
                     /gene="yajC"
                     /locus_tag="PLT31_06020"
     CDS             5924..6268
                     /gene="yajC"
                     /locus_tag="PLT31_06020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HPW94730.1"
                     /translation="MKLFPLILALVATTPLFAQEAAPQGGGIMAFLPFILMFVVMYLF
                     FIMPKQKEMKKMDAMRKALKKGDKVLTTAGIIGLVSNIEEGVVTIKTGDATKIDFEKA
                     AILRVLDSKTEN"
     gene            6281..>6548
                     /locus_tag="PLT31_06025"
     CDS             6281..>6548
                     /locus_tag="PLT31_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPW94731.1"
                     /translation="MDILDIKIYGDDVLRQKCKPVEKLTPELVEIAKQMLETMYDAPG
                     VGLAAPQVGLDIQLVVIDVTHPDDDERCPYIMFNPEWKEEEDSEP"
BASE COUNT         2079 a         1117 c         1320 g         2032 t
ORIGIN      
        1 ggcaagaggc ttaattaata aaagtattgg cgcactaata caagcaatga taccagcctg
       61 agttaaaact ttagcgcaat tctcaacacg ttctgccccg aggttctgag caacaagggc
      121 ggtggcataa ctgatcgttc ctgtaaaaaa ggtaaggatc atcatttgag taatcccacc
      181 acttaaggcc gcgttcatat attcagagcc aacacgggaa agaaaaagac gatcagcaaa
      241 ggtcattatg gtgtcaaaag acatggataa aagcatggga agtgctacta cgagcacgtc
      301 tttgattcca cccttctttt gcaaattctt tttcatgaag agtaaagtta gaaataatta
      361 aatttatttt aactatattt cgccattatg ttttctcaac ttacagactc tttagaatcg
      421 acccttaaaa aactacgtgg ccaaggaact ttgaccgaag aaaacgtcgc cgatgctctt
      481 cgtgaagtcc ggcgtgcttt tcttgaagca gacgttaact ttaatgttac ccgagatttt
      541 gtcaaagcgg ttaaagaaaa agcgcttggc accgatgtat tgctttctgt aactccaggg
      601 caacaaattg ttaaaatcat caacgatgaa ttaatccaaa tgatgggagg ggagaccaaa
      661 gagttacaac tcacaggagt tcctcctact ggaatcatga tggttgggtt gcaagggtct
      721 ggaaaaacga cttttgctgg aaaaattgcc ttatacatga gaagtaagcg taagcgtaag
      781 ccattacttg tggctgcaga cgtttatcga ccagcggcta ttaaacaatt acaagttctt
      841 ggtaaatcca taggcatacc agtatacgaa gaaggccaag gcaatccggt ggagattatc
      901 aaaaatggtc atcgttatgc tattgagaat gggtacgatt tagtcatcta tgatactgct
      961 ggccgtctac agatagatga tgttttaatg caggagttgg aacaagctcg agatgcggtc
     1021 catccagaag aaattctttt tgtcgccgat gcgatgattg gtcaagaagc cgttagtgtt
     1081 gctgaaacgt tcttccagag gctcaatttc acaggtgtct gtctttctaa gatggatggt
     1141 gatacgagag gtggtgcggc gctttccatt aaaaagatga cgggcgttcc tatttgtttt
     1201 gtcggtcttg gagaaaaact ttctgagata gacttattcc atccagaccg tatggctagc
     1261 cgtatcttag gtatgggcga tgtcgtaagc cttgtcgaaa aagcacagtc ggtaatcgat
     1321 gaaaaagatg ccaaagactt aaagaaaaag atttttaata acacgtttga tcttaatgat
     1381 ttcttaaagc agcttcgtac aataaaaaaa ttaggtcgca ttaaggatat catgggtcta
     1441 attcctggat taaataagtt gcctttagat cagattaatg aaaaagaatt ggtctatgta
     1501 gaagccactt taagttctat gacgcctaga gaacgcaaaa agccacaaat tatcgatagt
     1561 agtcgaaaaa aaagaattgc tcaaggttct ggaacttctg ttcagaaggt gaatcaggtg
     1621 ctttctcaat acgattctat gaaagaaatg tttaagaaaa ttggtggcat ggctcagaaa
     1681 caaaatgggg gaactcctgt gggttctaac tatacgcctc ctaaagtgaa gaaaaagcga
     1741 aaatagctct attttttttg attttagtcg ctaaaaggcc ttttttttat gaaattaggc
     1801 tttttttgtg tttctaagca ttactttata tgtaaaagac ggattttttt ttgtttccgt
     1861 cctttacata caacttctca ttgattatat ttggttcaaa atttaatcag aggtataaaa
     1921 tggcaaccgt aattcgcctt tctcgttttg gtaagcgtca tcacgctgtt tatcgtgtag
     1981 ttgtagttga taatcgcaaa gctcgcgacg gtcaatttat tgaacaaatt ggtttctaca
     2041 atccaaacca agctacacca gacatcaact ttgatcaaga aaaagtttta aaatggttaa
     2101 gtgttggtgc tcaaccatct gatactgtag cttctcttct tcgcaaccaa ggtattatgg
     2161 atcttttcca tgaactaaag gcaaagcgtt ctattgaaaa taaggctgct actccacgtg
     2221 caaagaaagc aaaaaaagct cctttaggtc ctaaagcaaa agcaagatta gaagctgaaa
     2281 aggctgctaa agaagctcca gcaactgaag ctcctgctac tgaagcctaa ttaatagaat
     2341 tgaaagcctt gtgtttaaac acagggcttt tgtatttata aaaaatggaa aaggatttac
     2401 ttgtcattgg tcaattcatg cgaccacacg gagttcaggg ctatattaaa tgtaagcctg
     2461 aaacacacga tctcaatcgt tattctcggt tgacaagcct tttcgtatat caaaaaggca
     2521 ctcttctatc tttagaaata gaagatgcta aaattcaaag tggtgtatgg ttgtttaagt
     2581 ttaaggggta tgattctcct gagtctataa agccattcac taatctagaa gcatgtattt
     2641 caaattccga aagaattcct gctccaccag ggcagattta tttttcggac gctgccgatt
     2701 tccaagtcgc cctcgaagat ggtactattg ttgggaaagt tttggaaatg atagaattac
     2761 cttctgttaa tgctttttgc attcggttta attctcctta taacgaacaa tttacaaatc
     2821 aagagatcct cgccccatgg attgatgctt gtattatcga tataaatgag aagtctaaaa
     2881 tcatattctg tgatgagctg tttttgaaaa gtttatgcga aaatggagat tctaaatgaa
     2941 gatccaatgt ataacacttt ttccagaaat gtttgctccc atggattatt ccattatagg
     3001 acgggctcgt caaaagaaga ttttttcttt tgaaacgatt tatttgcgtg actttgctat
     3061 taatgagtat ggtcaagtag atgattcccc ttatggtggt gagcctggaa tggttcttcg
     3121 gccagaacca cttacaaaag caattcgaag taccggagtg aagaataatg gtggtaaagt
     3181 cgtttattta acggcagatg gtgttccttt cactcatgaa ttagcccttt ctttatctaa
     3241 agaaaaacaa atagttttcg tctgtggcca ttacaaaggc attgatgaac gcgtccgcga
     3301 atcagaaatc gatcttgaaa tttcaattgg tgattttgtg gtgagtgggg gcgaactacc
     3361 tgcaatggtg gtcaccgatg caattgttcg gcttttagat ggttctttag gaaataaaga
     3421 atctggagag acggattctt ttgcccaagg tcctcttggt tggccggttt acactcgccc
     3481 agaggaattt gagggcaaaa aagtgcctga aatccttctt tctgggcatc ataagaagat
     3541 tttggagtgg caaaaggcag aatctttaaa aagaacgcaa gaaagacgcc ccgacatcta
     3601 cgcaaaattg aaaatagata ctaaatttgg catcaaataa acgaggtcac actatgtccc
     3661 tgaatatcga agttattcat caagaaaata aaaaaactga acaagcagat ttccgtgcag
     3721 gtgattctgt tactgtaaac gtaaaagtga ttgaaggtac taaagaacgc attcagcctt
     3781 ttaaaggcgt tgtgattcaa aagaagaata ccggcattgc tactacatta acagttcgca
     3841 aaatgtctgg taacgttttt gtagaacgta ttttcccagt tcatagccca cgcattgatt
     3901 ctattgttgt ggatcgtcca ggtaaagttc gtcaagcacg catttattat atgcgcgatc
     3961 ttcgtggtaa ggctgctcgt atttcagaac gtaaggttta attcgagcgc tttatgcaaa
     4021 aagtgatccc gcccgtgcag atgtgcgtta aggcgggttt tcttgttttt aaacctggag
     4081 aatcaccaag gcaattaatc attgtagatg atggtaaatt aattgcttat gaacttattg
     4141 atggtgaaaa gcatattgct tttgaaatga atccaggtga cttgattggg gtggccgctc
     4201 ttcttgaaaa agaagaagtg cgttatcaaa tagaagctgc agtagattct aagatcacga
     4261 tcattgatga agagtgtatg gagtctcaat taattaagac acccgtctgg cttgtcgcat
     4321 taatgaaatc gctttctctt cgtacgcgag aattaaaaaa attaaaaaag caaacgggct
     4381 ctttgaatac gcttcaaagt ttgactcttt tcctttatta taaaaatcaa aaagagcttc
     4441 ctttattgga taccattcga gaatactgct ttcaaactag atgcgctata aaaacggctt
     4501 atgcagatct aaagcgtctt caacgtaaag atatcattta tctaaaaggg gataacgaag
     4561 aatctatttt aattcgtgag cctcttttac tttctattct aacagattgt ttcttatgta
     4621 agcaaacgca atcgccttat tatccttttc atttaagttc acttgaaaaa gaaggtctta
     4681 tattcctttc gaaatactct tcttctgaat ctcttgaaag ttctacttgg cttcaaattc
     4741 ttcaaaaaca gaatccttta ttctcagctt cagagtggat tcgttttcaa gaatatcaaa
     4801 tttttgttaa agatccagaa aataagtatt ttattcatca gaaaaaaatt caagaaatac
     4861 gcttagcact tgaaaatgaa aagagtataa agaagattat tttatgcaat taaaagctgg
     4921 tgattatctt tttatggaag gctcttttga tgatcacttg tatatagtga aaaaagggtt
     4981 tcttacggca gaacgtcgag aacacgggat gactcaagtc caatggactt ttgagcctgg
     5041 ttctcttatt ggagaagggt ctcttttaga aaataggcct cacacccatg cggtaaaggc
     5101 ttccgtagat tcagaattaa ttgcattaga ccaaaacgaa ttaaaagaaa ctcttaacaa
     5161 agcaccaaat tggattgttt ctattgttta ttttttatct cgtcgtttta gagaagcaga
     5221 aaaaaaacat tctcaaaaca acatgattcg tgcattacct agccttcttt ttattttatt
     5281 tcagtcaccg cagaaaaaaa agtatgatgt aaaagaggtg gtcaaagacc tttttatttt
     5341 aacagggctc cgttcaaaag aagctttttc tctattaaaa atattagaat ctttaggttt
     5401 gtttaaggtt tttgatgagc aaattcagag caccaatact cgcgtaattc aaatgcttta
     5461 tgaggctctt ctatatagag cttctaaaaa agaaattcca gcgacgattc tttctattac
     5521 agatcagttg atattaaatg cttttatcca aacgactaaa aagaatggga ctatatataa
     5581 ggggcaaaca gcgatagata cggctgtatt ttttaagaca ggtcctagat atgcacatgg
     5641 tttacgcttt tctttaaaat ccctagacga tctattacat aaaaaaatac taacccatgt
     5701 tgcagaagga tcgtcagatt tcgtttatgg ggatatagat ataattgtgg atttattaga
     5761 gttgaatcgg atttatcctc ttctagataa gaaattgatc gaatcgcctt aaacgaatac
     5821 gttaaataag cattttatca gatccttttg attggtagct gttctatttc ttttgatagt
     5881 tttctatttt ttagccagaa aaaatattta accaaggttt attatgaaac tatttccttt
     5941 gattcttgct ttagttgcta ccactccttt atttgctcaa gaagcagcac ctcaaggcgg
     6001 tggtatcatg gcatttttac catttatttt aatgtttgtg gtcatgtact tgtttttcat
     6061 tatgccaaag caaaaagaaa tgaagaaaat ggatgctatg cgcaaggcgt tgaaaaaagg
     6121 cgataaggta ttaacgactg cagggattat tggtcttgta agtaacattg aagaaggtgt
     6181 agtgactatc aaaactggtg atgccacaaa aattgatttt gaaaaagctg ctattcttcg
     6241 agttcttgat tcaaaaacag aaaactaagt ataggcgttt atggatatac ttgatataaa
     6301 aatatatgga gacgatgttc ttcgtcaaaa atgtaagcca gttgaaaaat tgacgcctga
     6361 gttagtggaa attgcgaaac aaatgcttga gactatgtac gatgctccag gcgttggttt
     6421 agcagctcct caagtggggc ttgatattca actcgtggtg attgatgtta ctcatccaga
     6481 tgatgatgaa cgttgtcctt atatcatgtt taatccagaa tggaaagaag aagaagattc
     6541 agaaccag
//
LOCUS       DAOOBB010000110         6545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_13864_length_6545_cov_4.282897, whole genome shotgun sequence.
ACCESSION   DAOOBB010000110 DAOOBB010000000
VERSION     DAOOBB010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 6545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6545
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13864_length_6545_cov_4.282897"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="PLT31_06030"
     CDS             complement(<1..1089)
                     /locus_tag="PLT31_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94732.1"
                     /translation="MFLFRLLPLFTLLICACPILASSFDLSFYDLKKDGFCSKQKAKF
                     DFSFHNKCFTANGKQKILSPFVAEQIRIRFIDPPKNEKASSTFYIEKPFLIIDGIHLS
                     TEEKRSLEDFEQDANAYGFPQVLKAFGYTPILVQFPRTVEKTLQANANSLKKMLYYLS
                     DNKVYLWPQMKDDSFILLGISQGGVIGRYAAYLYDSQKPSHFPKIKMFSSLDSPHQGA
                     VMPRGVLAAIDFWARGAKQGAAEDFLDLVSGPGAQQLLIYNTKKTTSKSANDFAPNLD
                     QKRFLFNEYRKAANYKGFPSILISQGQLKGVPNKRPNKAYYQLNRYAEKGSKVWGRAT
                     SLSNFTSDNSQVFAENRIYEYMDTDNKWT"
     gene            complement(1089..2360)
                     /locus_tag="PLT31_06035"
     CDS             complement(1089..2360)
                     /locus_tag="PLT31_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94733.1"
                     /translation="MLNRVFICVWFTCIVLASSIHSAECKSISLYSSNNPSPGFLTNS
                     SFPENPEWQANWGKLGEMEPPYIRFSGQHNQTKNWKAVISFPQMPFYVSSGFLELKVR
                     ATQKVQIKIALEQAENNGLAYTTSINANQTHKLEIPISSLLSKSSLIVKGVSIELIQI
                     PANQYTTLFLDDISFSCTSTTPITNTVLKSSDTLFVFSETIAEKAARTPLDTGAMPIS
                     YALKKHSDSAQIVLRSLSENQIVMTENEHHILQAKVLEKPSSPKQSWKSWNECLYYIN
                     KNRLNDSLFANPKVLFREANNFSASYNYQVIPILVGLFNYDYYLCNQKNSDSICIQKH
                     LESSKIMVSGFSSSFIYGSKVEFILDPYFITTNQKTMPEIELFLHNQWVSLKIRSRIT
                     HSFDSLGLHSIPVKLYHGSDIIETTLLLEVR"
     gene            complement(2820..>3667)
                     /locus_tag="PLT31_06040"
     CDS             complement(2820..>3667)
                     /locus_tag="PLT31_06040"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPW94734.1"
                     /translation="INIFLVFIAMSFRDLMIFLLTNPGPISITSAIAHFFDSKEVFAY
                     SRTERFQVFVLFLVLVFINCGINIAFSVSLRQTKHAQWFRYREKMALQTELSFLKTQL
                     SPHFLFNTLNNITALIDTNSSHAKKAIVQLSKLLRTILYETKTDYILLEKELEIIKKY
                     TDLERLRLPEDFDFELTTTIENKEAKITPLIFMPLVENAIKHSFCPSLKNYIHVQIQE
                     KNGFITFISENSCCPSTKHKNTQGGIGLNTFIKRMDSFYLNEYSYKATQEENSYKVFL
                     RFKSR"
     assembly_gap    3668..3677
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3678..4002)
                     /locus_tag="PLT31_06045"
     CDS             complement(<3678..4002)
                     /locus_tag="PLT31_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94735.1"
                     /translation="MNQNDYQGFSFSEKDRKRALQYDADFEHLLTHFKSFKKPIPNFL
                     PVVLAWIFIFTFPLLLMMDYRNKPLIENLTSVISIYIPLIFAALIFFLNQAFLIPSYF
                     IRGRIK"
     gene            4217..5074
                     /locus_tag="PLT31_06050"
     CDS             4217..5074
                     /locus_tag="PLT31_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HPW94736.1"
                     /translation="MSLKSTKEWNDRDIYFQYLKDISKYPLLTREQETILLRRVSEGD
                     KLALDLLVKSNLKFVVNIANLYKGQGLEVNELINEGNMGLIEAARRFDPNQKIKFISY
                     AVWWIRQNITRAISEKARIVRISAEKELVLRRFNRVGVKTRQVLGGQVTIDPASLEGS
                     SRYNAEEIEKILMMGSRTSSLDAPVGEDGEMTLGECIAVETDMTEDFAMEKNRRKVFK
                     KLLQENLTEQEHQIIGLYYGLDVDADLNLKEIGSMMGLSKERVRQVKETALAKLRASR
                     VEQIIGEAA"
     gene            5187..>6545
                     /locus_tag="PLT31_06055"
     CDS             5187..>6545
                     /locus_tag="PLT31_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPW94737.1"
                     /translation="MKFQTPYTRFFSIITVFLFFMATLSFGANDKKEPGDFYEELSRL
                     NKVLSEINKKYVEEVDPAEISEAALQGIKNILDPHTVVFGPKDYENLKVSMEGEFGGV
                     GITISLRDNILTVVSPLAGTPAFNLGIRAGDRIRKIEGKDTKGMTLDEAVSQLRGKVG
                     TDVTIAIEREGVPELMDFTITRAKIVVHAVPYSGMLDKSIGYIKLATFSDKTTFEVER
                     AIENLKKQGMKKLILDLRFNPGGLLNQAIEIGELFLKKGNVIVSTRGRTQKTESFARR
                     DGILGPEVPLVILVNQGSASASEIVAGAIQDWDRGLIIGKTSFGKGSVQTIFPLDRDG
                     NALKLTTAFYYLPFGRCINKPENGIKGQSLLEEQETEMSTDSLAAKDTVKVDTFTTNN
                     GRKMFGAGGISPDIDIELPPMPWVVQVQERMAMYFKFAVKIRPALDASGTKIDANWVV
                     PDS"
BASE COUNT         2165 a          968 c         1256 g         2146 t
ORIGIN      
        1 tgtccattta ttatcagtat ccatatattc gtaaattcta ttttctgcaa aaacttggct
       61 attgtcagaa gtaaaattag ataggctagt cgcacgaccc caaacttttg atcctttttc
      121 agcatagcga tttaattgat aatatgcttt attaggacgt ttatttggga cgcctttcag
      181 ttgcccctga cttattaaaa tagatgggaa tcctttataa ttagcggctt ttcgatattc
      241 attaaataaa aagcgttttt gatccaaatt aggtgcaaaa tcattggcac tttttgaagt
      301 tgttttctta gtattataaa tcaataattg ttgtgctcct gggccagaaa ctaaatctaa
      361 aaaatcttca gcggcgcctt gttttgctcc tctagcccaa aaatcaatag ctgctagaac
      421 acctcttggc attacagctc cttgatgagg ggagtctagc gaagaaaaca tttttatttt
      481 aggaaaatga gaaggttttt gtgaatcata taagtatgct gcataacgac caatgactcc
      541 accttgacta attcctaaaa gaataaaaga atcatccttc atttgtggcc ataaatagac
      601 tttgttatcg gataaataat aaagcatttt ttttaatgaa tttgcatttg cttgcagcgt
      661 tttttctaca gttcttggaa attgaacaag tatgggtgta taaccaaaag cttttagaac
      721 ctgaggaaaa ccatatgcat ttgcgtcttg ttcaaaatct tccagagaac gtttctcctc
      781 tgtacttaaa tgaatcccat caataatcaa aaaaggcttt tctatataga atgtcgaaga
      841 ggccttctca tttttaggag gatcaatgaa tcgaattcga atttgttctg caacaaacgg
      901 ggaaagaatt ttctgtttcc catttgctgt gaaacattta ttatgaaaag aaaagtcgaa
      961 ttttgctttt tgcttgctgc aaaatccatc tttttttaag tcatagaagg ataaatcaaa
     1021 agaagaggct aaaatagggc aagcacaaat caagagagtg aaaagaggta atagtctaaa
     1081 taaaaacatt agcgaacctc caaaagaagg gtggtttcaa tgatatcact accatgataa
     1141 agttttacag ggatagagtg aagaccaaga gaatcaaaag aatgggtaat tctagagcga
     1201 atcttcaaag agacccattg attatgaaga aagagctcta tctctggcat tgttttttga
     1261 tttgttgtaa taaagtaggg gtcaagaatg aattctactt ttgaaccata aatgaaagaa
     1321 gaactaaagc ctgaaaccat tatttttgag gattccaaat gtttttgaat gcaaatggaa
     1381 tcactatttt tttgattaca gagataataa tcataattaa agagacccac taaaattgga
     1441 atgacttgat agttataaga agcagaaaag ttatttgctt ctctaaataa aactttaggg
     1501 tttgcaaata gactatcgtt taatcgattt ttattgatat agtataagca ttcattccag
     1561 cttttccatg attgttttgg gctagaaggt ttttctaaaa cttttgcttg taaaatatga
     1621 tgctcatttt cagtcatcac aatttgattt tccgaaagac ttcttaaaac tatttgagct
     1681 gaatccgaat gcttttttaa ggcataactg atgggcattg ctccagtgtc taatggcgtt
     1741 ctggcggctt tctctgcaat agtttcgcta aaaacaaata atgtatcaga acttttcaaa
     1801 acagtatttg tgatgggagt tgttgaagta caagaaaaag aaatatcatc taaaaaaaga
     1861 gtggtgtatt gattcgcagg tatttgaatc aattcaatag aaactccttt tactattaaa
     1921 gatgattttg aaagtaacga gcttataggg atctctagtt tatgtgtttg attggcattg
     1981 attgaggtcg tgtatgctaa gccattattt tcagcttgtt caagagctat ttttatttgg
     2041 actttttgag tcgcccgaac ttttaactct agaaatccac ttgaaacata aaatggcatt
     2101 tgaggaaaag agataaccgc tttccaattt ttagtttgat tatgttggcc tgaaaagcga
     2161 atataaggtg gttccatttc tcctaattta ccccagttcg cttgccattc aggattttct
     2221 gggaacgaag aattcgttag aaaacctgga gaaggattat ttgaagagta aagagaaatg
     2281 gatttgcatt cagccgaatg gatacttgat gctaacacaa tgcaagtaaa ccatacacaa
     2341 ataaatactc tattaagcat agagtctcct taaggtgtat taaggtagct cattacaatt
     2401 caagaaaata ttgtggctat actaactcgg aaaataacaa ggaatcaact cgacaagctt
     2461 gttatgagtt ttattttctc gatacgttta gtcaacaact cttttcaaaa aaagaattat
     2521 tagagctccc tgtagtgaat atagcgaaga ggccaaagcc gatatagaat agaacgtccg
     2581 attggacgga gtgccaaaat agaaaattaa tttgtaaaga aaagaaaaat aatatttact
     2641 ttatgatctc aggaaatcgt cttatcggat tttgttgttt actttatgat cttaggaaat
     2701 catcttatca gattttgttg tttactttat aattttacga aatcgtctta tcggattttg
     2761 ttgtttactt caaatttatt ggctgccgct ttatcggaaa acttacgtat ctcaattgtt
     2821 tatctacttt taaatctcaa aaatacttta tatgaattct cttcttgcgt tgctttataa
     2881 gaatattcgt tcaagtaaaa agaatccatt ctttttataa aagtattcaa gccaattcca
     2941 ccttgtgtat ttttatgttt tgtagatgga caacaagaat tttcagatat aaaagtaata
     3001 aaaccatttt tttcttgaat ttgaacatgg atgtaatttt ttaatgaagg gcaaaaacta
     3061 tgcttgatcg cattttcaac aagaggcata aaaataagtg gtgtgatttt tgcttcttta
     3121 ttttcaatag ttgtagtaag ttcaaaatca aaatcttcag gtaatcgtaa tctttctaga
     3181 tcggtgtatt ttttaataat ttcaagctct ttttctagaa ggatgtaatc tgttttagtt
     3241 tcataaagaa tagtacgaag taatttagaa agttgaacaa tggctttttt tgcatgagaa
     3301 gagttcgtat caataagagc tgtgatgtta ttaagagtat taaataaaaa atgagggcta
     3361 agttgtgttt ttagaaaaga cagttctgtt tgtaaagcca ttttttcacg atatctaaac
     3421 cattgagcat gctttgtttg tcggagacta acgctaaaag caatgttaat tccacaatta
     3481 ataaaaacta gaactaaaaa caaaacaaat acttgaaaac gttctgttcg actataagca
     3541 aaaacttctt ttgaatcaaa aaaatgagca atagcgcttg taatagatat gggaccagga
     3601 tttgttaata agaaaatcat aaggtctcta aaagacatcg caataaagac aagaaaaata
     3661 ttgatgannn nnnnnnnatt ttattcgacc acgtataaag taactaggga ttaagaaagc
     3721 ttgatttagg aaaaaaatta gtgcggcaaa aataagagga atataaatag aaataacgct
     3781 tgtcagattt tcaattaagg gcttgtttct ataatccatc attaataaaa gaggaaaggt
     3841 gaatataaaa atccaagcaa gaacaacagg aagaaagttc ggaataggtt tcttgaatga
     3901 cttaaaatgt gtaagtaaat gctcgaaatc agcatcgtat tgaagagctc ttttgcgatc
     3961 tttttcagaa aaagaaaaac cttgatagtc gttttgattc atattagact cttatttcgc
     4021 ctctttttca ttaatttaag aacaaatttg gtgctaaata gtgttttgtg acattttttt
     4081 gacgaaacta cgtaaataaa cgacaaaaat acgattgaag tattgcgaaa tgactcatga
     4141 gttctgacac ttggatgttt caagtgagtt agatttaata aagtcaagtt aagatcaaca
     4201 atatcgtgag gttaacatga gtcttaaaag cacaaaagaa tggaatgacc gtgacattta
     4261 ttttcaatat ttgaaagata tctccaaata tcctcttttg actcgtgaac aagaaacgat
     4321 tttattaaga cgggtatctg aaggagataa gctagcctta gatttgttag ttaagtctaa
     4381 cttgaagttt gtggtcaata ttgctaatct ctataagggc cagggacttg aagtgaatga
     4441 attgattaac gaaggaaata tggggcttat tgaagcagcg cgtcgttttg atcctaatca
     4501 aaaaatcaag tttattagtt atgctgtatg gtggattcgt caaaatatta ctcgtgctat
     4561 ttcagaaaaa gcaagaattg ttcgaatcag tgcagaaaaa gaattagttc ttcgtcgttt
     4621 taatcgcgtt ggagtgaaaa ctcgtcaagt tttaggcggt caagtgacta ttgatccagc
     4681 aagtttagaa ggatctagtc gttataatgc tgaagaaatt gaaaagattt taatgatggg
     4741 aagtcgtact tcttctttgg atgctcctgt tggtgaagat ggcgaaatga ctttaggtga
     4801 gtgcatcgct gttgaaacgg atatgacgga agatttcgct atggaaaaaa atcgtcgtaa
     4861 agtttttaag aagcttcttc aagaaaacct aacagaacag gaacatcaaa ttattggttt
     4921 atattatggt cttgacgtgg atgctgattt gaatctaaaa gaaatcggct ctatgatggg
     4981 attatcaaaa gaacgtgttc gtcaagttaa ggaaactgct ttagcaaaac tcagagcgag
     5041 tcgtgtagag cagatcattg gcgaagcggc ttaatttcga ttttattttt ctcttttttc
     5101 tctcaatatc taataaatcc agctgaaaag ctggattttt tccgttttta aattactttg
     5161 atttgttgaa attgtatctt tataagatga aatttcaaac tccatataca cgcttttttt
     5221 caataatcac agttttctta tttttcatgg ccactctttc ttttggtgcc aatgacaaaa
     5281 aggaaccagg tgatttttac gaagaacttt ctcgcttaaa taaggttctt tcagaaatca
     5341 acaaaaaata tgttgaagag gtcgatcccg cagaaatttc tgaagctgct cttcagggaa
     5401 tcaaaaacat actggatcct catacagttg ttttcggacc aaaagattat gaaaatttaa
     5461 aagtatccat ggaaggcgag tttggtggtg ttggtatcac gattagtctt cgagacaaca
     5521 tacttacggt tgtttcccct ttggctggta cgcctgcctt caatctcggt attcgtgcgg
     5581 gagaccgtat tcgtaaaatt gaagggaaag ataccaaagg aatgaccttg gatgaagctg
     5641 ttagtcaact gcgaggcaag gttggtactg atgtgaccat agcaattgaa cgtgaagggg
     5701 ttccagaatt aatggacttt acgattacgc gcgctaagat tgttgttcat gcagtgcctt
     5761 atagcggtat gttagataag tccatcggtt acattaagct tgcgactttt tctgataaga
     5821 ccacttttga agtggaacgc gccattgaga acttgaagaa acaaggaatg aaaaaattaa
     5881 ttttggacct tcgttttaat cctggtggcc ttttaaatca agctattgaa attggagaat
     5941 tattccttaa aaaaggaaat gtcattgtaa gtactcgtgg acgaactcaa aagacagaga
     6001 gctttgctcg aagagatggt attcttggtc ctgaagtgcc tttggtgatt ttagtgaatc
     6061 aaggttctgc aagtgcctct gaaattgttg caggagctat tcaagactgg gatcgaggcc
     6121 ttattattgg taaaacgtct tttggaaagg gttcagtaca gacgatattc ccattggatc
     6181 gtgatggaaa tgctctaaaa ttaacaactg ctttttatta tttgcctttt gggcgttgca
     6241 tcaataagcc agaaaatgga attaaggggc agagtctttt agaagaacag gaaacggaaa
     6301 tgtcaacaga ttcacttgca gcaaaggata ctgtaaaagt ggatacattt actacaaata
     6361 atggccgaaa aatgtttggt gctggtggta tctctccaga tatagatatt gagctacctc
     6421 cgatgccttg ggttgttcaa gtgcaagaac gtatggcaat gtattttaaa tttgctgtta
     6481 aaattaggcc tgctttagat gcctctggca ctaagataga tgcaaattgg gtcgttccag
     6541 attct
//
LOCUS       DAOOBB010000111         6535 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_13888_length_6535_cov_3.711420, whole genome shotgun sequence.
ACCESSION   DAOOBB010000111 DAOOBB010000000
VERSION     DAOOBB010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 6535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6535
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13888_length_6535_cov_3.711420"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="PLT31_06060"
     CDS             <1..891
                     /locus_tag="PLT31_06060"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94738.1"
                     /translation="TAIQNNWGVCLALEKGLGIGVPERGEYIRVMLAELGRIASHLVF
                     YACFGGDLGGQTALLFGFKEREMIHDILDEVTGSRLTTNFFRPGGSVHDVPDTFIPRV
                     TSFLKHLEKTMVDYERFLSTNIIVLERTKGIGILSKEDAIAYGCSGPVLRASGVSYDV
                     RKNDPYGIYSQLDFNVPVTYNGDTYDRYVVRIAEIHESIKILRQCIKQFPEGPYRGQE
                     KASIRLKPGSYYSATETAKGLYATYVVAVSGDKPYRIHTRGPSFANLAALNTMVQGHK
                     ISDLVSILATLDPVIPEIDR"
     gene            888..1964
                     /locus_tag="PLT31_06065"
     CDS             888..1964
                     /locus_tag="PLT31_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HPW94739.1"
                     /translation="MIDPVISHPLGDFIREIIPIPEISFLINALISIIAICAVAIGTA
                     PILIYMERKICAHIQCRLGPMRLGFHGTLQTIADVMKLLFKEVYAPKGADKFLFYLAP
                     MISLTAPFLALTMIPFDHNLQVANLSVGIPFIIAVNGFGILGILIGGWASNNKYSLLG
                     SLRSGAQMISYEISFALVLLFVVMVSGSANLMDITQSQSGTILDWWIFKIPVLGFIAF
                     IMFFISSTAELNRAPFDIAEAEQELTGGYHTEYNGTAFAMFYLAEYINLVTTSSLATT
                     CFLGGFYPPLIGIESIDTLLLKVPGFIWFAGKVFFMISAYMVVRWTFVRPRVDQLMDF
                     EWKFLLPANLLLLALGATYIAIIG"
     gene            1970..2452
                     /locus_tag="PLT31_06070"
     CDS             1970..2452
                     /locus_tag="PLT31_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPW94740.1"
                     /translation="MKQKVSPISTAEYIKRYAKRCITGPISLLKGLSVTVRYFFSPHK
                     IVTEQYPENKKTLKMHSRYRGCLELIEDENGENLCTACGICEKACPNASINILSTKNI
                     AGKKVLGKYLYHLGSCTQCGLCVEACPFGAIRMNQEFELSNESVDSFEMTLNKKEGRG
                     "
     gene            2452..2985
                     /locus_tag="PLT31_06075"
     CDS             2452..2985
                     /locus_tag="PLT31_06075"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HPW94741.1"
                     /translation="MFPLETVDIFFYIVSTIIIVTAILTVTAKNVLQSAIFLIFSFIG
                     TAVFYLMLHAEFNAIAQVMIYAGGVVIFVVFTILLTSRLGETSLPIKIPRSFIAAVIS
                     LSLVFVMWRFLLPDASLIRTLPGANSDFASLESIAIRLLGIDANGFVIAFELISVLLL
                     VTLICSITIARKSKENK"
     gene            2982..3287
                     /gene="nuoK"
                     /locus_tag="PLT31_06080"
     CDS             2982..3287
                     /gene="nuoK"
                     /locus_tag="PLT31_06080"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HPW94742.1"
                     /translation="MTLLNCLLLSFILFGIGVWGLLRRRHLVAMLISIELMLNAANMN
                     FITFAYFSSGDSTAGAVFNLFVVAVTACEMAIALAIVVSMYRKRHSLDVNELRSLHE"
     gene            3280..5241
                     /gene="nuoL"
                     /locus_tag="PLT31_06085"
     CDS             3280..5241
                     /gene="nuoL"
                     /locus_tag="PLT31_06085"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HPW94743.1"
                     /translation="MNNLTTLSYFIFLLPLFSFLINGLFLKSSYRKTAGFISVILNGF
                     AAFIAIYLSYVYFASGIAPGRMILWENNFLVFSSSLKASIGLLLDPLSAMMLVVITFI
                     SFVVNIYSIGYMKEDRSTTRFFSFLSLFTFSMLGLVVAPNLFQMFFFWELVGISSYLL
                     IGFWYEKPGAVSASKQAFILTRFADSFFLLGIVLASYIIGSFDFFALNTLSLADFLDE
                     INLGIISISKAQALFVSSILIFIGGWGKSAMFPMHIWLPNAMEGPTPVSSIIHSATMV
                     VAGVYLVARLFPFFSLFSDTLSLIMTVGIFTALFAAVIACTQKDIKRILAFSTLSQLG
                     YMMFALGSTSVLINGEMAINTLGYSASIFHIFTHAFFKCMLFLIAGSLIHAVHHNDLD
                     SMGGLGKKMPWTYAAALIGCLAISGIPPFSGFFSKDEILIAALQGKHFIVFGLALLTS
                     GLTAFYMFRFFFLAFHGNARSVHTAHVKESFSMTLPIVILAIPSFLGGYLFKKVFLKF
                     FTPGYIPTSNVSESLHSANWVPFAAAIIALIGIFLAWVFYARPYANVKKALDETNRKT
                     FYKWIYHKFYFDEIYYAFVRQFIFKGVAATIRFFEDYVVGGAVKGSVFIIKKAGELVR
                     EAQSGFAPFYIGTLIVGLLLWRFLGNLPV"
     gene            5251..>6535
                     /locus_tag="PLT31_06090"
     CDS             5251..>6535
                     /locus_tag="PLT31_06090"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HPW94744.1"
                     /translation="MIDTLLFNSIWILPLLTTLICIPIGVHHSEAIKRIHLGSAFVVL
                     GIALYLTIKTFLLANTAINSTGSTFSLNFVTQFSWWKTIGAEYFVGADALTLLMVLLT
                     AIVVFTGMLVSWSIQGNTKIFFALIQILGTSIYGVFMSFDLFLFFIFYEMEALCMYLM
                     IGGFGSGNKDYGAKKLTLTLALGSSMVLAALFGIYVESGIDSWNLLKLGDVVLPMSLQ
                     MWAFPLLFMGFAVSGSLFPFHFWSPDGHSAAPTAVSMLAAGVMMKMGPYACLRVAIFL
                     MPEAARIYLPYIVYLLIFNVLLSGFIAIRHKDLKYITAYSSISHLGLIFLGLAAMTPL
                     AFRGAVLQMLSHGFLTALFFATIGMIYSRTHTRDITLMGGLMKKVPFIGVGFVIAGFA
                     CLGLPGLSGFIAEATIFIGSFQNESSMMRIVTILAV"
BASE COUNT         1668 a         1187 c         1214 g         2466 t
ORIGIN      
        1 acagccattc aaaataactg gggtgtttgc cttgccttag aaaaaggtct tggaatcgga
       61 gttcctgaaa ggggcgaata cattcgagtg atgctagccg aacttggtcg cattgcttct
      121 catcttgttt tttatgcttg ttttggtgga gaccttggtg gtcaaacggc tctcttattt
      181 gggtttaaag aacgtgaaat gattcatgat attcttgatg aagtcactgg ttctcgctta
      241 acgaccaatt tctttaggcc aggtggttct gttcacgacg ttccagatac ttttattcct
      301 agagtaacct cttttctaaa acaccttgaa aaaacaatgg tagattacga acgattctta
      361 tctacaaaca tcattgtctt agaaagaaca aaagggatcg gcattctttc aaaagaagat
      421 gccattgctt atggttgttc tggccccgtt ttaagagctt ctggtgtttc ttatgacgta
      481 agaaaaaatg acccgtatgg aatttattct caattagatt ttaatgttcc cgtgacttac
      541 aacggtgata cttacgatcg ctacgtggtt cgaattgctg aaattcatga atccataaaa
      601 attcttcgtc aatgcataaa gcaattccct gaagggccat atagaggtca agaaaaagcg
      661 tctattcgct taaaaccagg ctcttattat agtgctacag aaacagcaaa aggactttat
      721 gcaacttatg ttgtcgccgt ttctggagat aagccttatc gaattcatac gagaggccca
      781 agctttgcaa atttagcagc cttaaataca atggtgcaag ggcataaaat atcggatctc
      841 gtttctattt tagcgacgtt agatccagtg attccggaga ttgacagatg attgatcctg
      901 taatatcaca ccctttgggt gatttcattc gtgaaataat tcctattcca gagatttctt
      961 ttttaataaa tgctctgatc tctattattg ctatttgtgc cgtagctatt ggtactgctc
     1021 ctattttgat ttatatggaa cgtaagattt gtgcccatat ccaatgtcga ttagggccta
     1081 tgcgtcttgg ttttcatggg accttacaaa caatagccga tgtgatgaag ttgcttttca
     1141 aagaagttta tgccccaaaa ggtgctgata aatttttgtt ttatttagct ccaatgattt
     1201 ctttgacagc cccatttcta gcattaacga tgattccttt tgatcataat ctacaagtgg
     1261 ctaacttatc cgttggaatc ccctttatta ttgccgtcaa tggctttggt attttaggaa
     1321 ttctaatcgg tggctgggct tccaacaata aatattcttt acttggatcc cttcgaagcg
     1381 gtgctcaaat gataagttat gaaatttcat ttgcactcgt tttattattt gtagtaatgg
     1441 tttctggatc tgccaatcta atggacatca ctcaaagcca atctggaaca attcttgact
     1501 ggtggatttt taagatccct gtacttggtt ttatcgcttt tatcatgttt tttatttctt
     1561 ctacagcaga actcaatcgt gctccatttg atattgcaga agcagaacag gaactaacgg
     1621 gtggctacca tacagaatat aatggtactg cttttgccat gttctatctt gctgaatata
     1681 tcaacttagt gactacttct tctttagcaa ccacatgctt tttaggtggg ttctaccctc
     1741 ctttaatagg tattgaaagt atcgatactt tattgttaaa ggttcctgga tttatttggt
     1801 ttgcaggtaa agtatttttc atgatttctg cttatatggt ggttcgttgg acttttgttc
     1861 gtcctcgtgt agaccaattg atggattttg aatggaagtt tttattacca gctaatttat
     1921 tattacttgc tttaggcgca acatacattg ccattatagg ttaattctta tgaaacaaaa
     1981 agtatcacct atttctactg ccgaatatat taaacgctat gcaaaacgtt gtatcacagg
     2041 ccccattagt cttttaaaag ggttatctgt taccgttcgt tattttttca gtcctcataa
     2101 aattgtcaca gaacaatacc cagaaaataa aaagacatta aaaatgcatt ctcgttatag
     2161 aggatgttta gaattaattg aagatgaaaa tggagaaaat ttgtgtacgg cttgtggcat
     2221 ttgtgaaaaa gcgtgtccaa atgcatcgat caacatactt tcgaccaaaa atattgcagg
     2281 taaaaaagtt ttaggaaaat atctttacca tctaggttct tgtactcaat gtgggctttg
     2341 tgtagaagca tgtccttttg gtgctattcg catgaatcaa gaatttgaat tatcaaatga
     2401 atccgtagat tcttttgaaa tgaccttaaa taaaaaggag ggacgaggct aatgtttcct
     2461 ttagaaactg tagatatctt tttttatatc gtttctacta taattattgt aacagccatt
     2521 ttgactgtca ctgcaaaaaa cgttcttcaa agtgccatct ttcttatttt ctcatttatt
     2581 ggaacggctg ttttttattt aatgcttcat gctgaattta atgccattgc tcaggtgatg
     2641 atttatgcag gtggtgttgt tatttttgtt gtttttacga tattactcac aagtcgtctt
     2701 ggagaaactt ctctccccat taaaattcct cgatcattta ttgctgctgt tatatcgctt
     2761 agtttagtct ttgtcatgtg gcgtttttta ttgccagatg catctttaat tagaacattg
     2821 ccaggagcaa attctgattt tgcttcttta gaatcgatcg ctatacgact cttaggtatt
     2881 gatgcgaatg gttttgtaat tgcttttgaa ttaattagtg ttctcttatt agtgacttta
     2941 atttgctcta ttactatagc tcgtaaatca aaggaaaaca aatgacttta ttaaattgcc
     3001 tattattgtc ttttatcctt tttggaattg gtgtatgggg tttacttcgt cgtcgtcatc
     3061 ttgtggcgat gttgatttcc atagaattga tgttaaatgc agcgaatatg aattttatta
     3121 ctttcgctta tttctcttct ggggactcta ctgcaggagc cgttttcaat ctttttgtag
     3181 ttgcagtcac cgcttgtgaa atggctatag ctcttgccat tgtagtatca atgtatcgta
     3241 aacgccatag tcttgacgta aacgaattga ggagtttaca tgaataattt aaccacccta
     3301 agttatttta tttttttact tcccttattc agtttcttga ttaatgggtt gtttctaaaa
     3361 tcatcctaca gaaaaacagc tggttttatt tctgttattt tgaatggttt tgcagcattc
     3421 attgccatat acctgagcta tgtttatttt gctagtggca ttgccccagg tcgaatgatt
     3481 ttatgggaaa ataacttttt agttttttct tcttctttga aagcaagcat cgggttactt
     3541 cttgaccctc tctccgccat gatgctagtc gtcattacct ttatttcttt tgttgtaaat
     3601 atctacagta ttggttatat gaaagaggat cgttccacga cacgattctt ttctttttta
     3661 tccttattta cattttcaat gctcggttta gtggtcgctc cgaatttatt ccagatgttc
     3721 ttcttttggg aattagtagg gatctcaagc tatcttttga ttggattttg gtacgaaaaa
     3781 ccaggtgccg tttctgcatc aaaacaagcc tttattttaa ctcgctttgc agatagcttt
     3841 ttcttattag gtattgtttt agcaagttac attatcggtt cctttgactt ttttgcatta
     3901 aataccttat ctcttgctga tttcttagat gaaatcaatc taggaattat tagtatttct
     3961 aaagcacaag ctttatttgt ttcttctatt ttgattttta ttggtggttg gggcaaaagt
     4021 gccatgtttc caatgcatat ttggcttcct aatgcgatgg aaggccctac cccagtttca
     4081 tcgatcatcc atagtgccac catggtcgtc gctggagtgt atttagtcgc tcgtttattc
     4141 cccttcttct ctttattctc agacacacta tctttaatca tgactgtcgg tatttttacc
     4201 gctctatttg cagcagtcat cgcctgtaca caaaaggata ttaaacgtat tctcgcattt
     4261 tccacgctta gccagcttgg ttacatgatg tttgctctag gttccacaag tgttctgatc
     4321 aatggagaaa tggcgatcaa tactctcggc tatagcgctt caatttttca tattttcact
     4381 cacgccttct ttaaatgcat gttgttctta attgcaggtt ctttaatcca tgcagttcat
     4441 cataatgacc tagattcaat gggcggatta gggaaaaaaa tgccttggac ttatgcagcg
     4501 gcgttaatcg gttgccttgc catttctgga attccaccat tttctggctt tttctcaaaa
     4561 gatgaaatat taatagccgc gttacaaggg aagcacttta tcgtttttgg tctcgcttta
     4621 ttaactagtg gcttaactgc attttacatg ttccgcttct tcttccttgc ttttcacgga
     4681 aatgctcgct ccgtgcacac cgctcatgtt aaagaaagct tttcaatgac tcttccgatt
     4741 gtcattttag ctattccgtc gtttttaggt ggttatctat ttaagaaagt ctttttaaag
     4801 ttctttacgc ctggttatat acctacttct aatgtttctg aatcacttca ttcagctaat
     4861 tgggttcctt ttgcagcagc gatcattgct ctaataggga tctttcttgc ttgggttttc
     4921 tatgcaaggc cttatgccaa tgttaagaaa gctttagatg aaaccaaccg caaaacattt
     4981 tataaatgga tctatcataa gttttacttt gatgaaattt attatgcgtt tgttcgacaa
     5041 tttattttca aaggcgttgc agcaaccatt cgtttctttg aagattatgt tgttggtggg
     5101 gctgtaaaag gttctgtatt tattattaaa aaagctggcg aattagtgag ggaagctcaa
     5161 tcgggctttg ctcctttcta tataggtaca ctgattgttg gtcttttatt atggcgtttt
     5221 ttaggaaatc ttcctgtata gggtaaaaat atgattgata cgttattatt taattctatt
     5281 tggattctcc ctttattgac tacattaatt tgtattccaa ttggagttca tcatagcgaa
     5341 gcgattaaac gaattcattt aggttccgct tttgttgttc taggaattgc tctttatctt
     5401 acgataaaaa cattcttatt agcaaatacg gccatcaatt caactggcag tactttttcc
     5461 ttaaactttg tcactcagtt ttcatggtgg aaaaccatag gagctgaata ttttgtgggt
     5521 gccgacgccc ttactctttt aatggttctt ttaactgcga ttgttgtatt cactggaatg
     5581 cttgtcagtt ggtctattca aggaaacacc aaaatctttt ttgctctaat tcaaatttta
     5641 gggacaagca tttatggcgt attcatgagc tttgacttgt ttttattttt cattttttat
     5701 gaaatggaag ccttatgcat gtacttaatg attggtggtt ttggttctgg aaataaagat
     5761 tatggtgcta aaaaactaac tctcacttta gctctaggtt cttccatggt tcttgcagcc
     5821 ctttttggga tttatgtaga atcaggaatc gatagttgga acttgttaaa gcttggtgat
     5881 gttgttttgc cgatgtctct tcaaatgtgg gctttccctc ttctatttat gggatttgct
     5941 gtttctggat ctttattccc ttttcatttt tggtcgccag atggccactc ggcagcccca
     6001 accgccgttt caatgcttgc cgcaggcgtc atgatgaaaa tggggcctta cgcttgttta
     6061 cgcgtcgcca tattcttaat gcctgaagcc gctcgcattt atctccccta cattgtatac
     6121 ttgttaattt ttaacgtact cttatctggt tttattgcaa tacgccataa agatttgaag
     6181 tacattacgg cctatagctc tatttcacat ttaggcctaa tctttttagg ccttgcagcg
     6241 atgactccac ttgcctttag aggtgctgta cttcaaatgc ttagtcatgg tttcttaacc
     6301 gctcttttct ttgcaactat cggaatgatt tattcaagaa cccacactcg agatatcacc
     6361 ttaatgggtg gtttaatgaa aaaagttcct tttattggag ttggatttgt aattgctggt
     6421 tttgcatgcc ttggattacc aggtctttct ggttttattg cagaagccac tatatttata
     6481 ggtagctttc aaaatgaatc ctctatgatg agaatcgtta ccattttagc ggttt
//
LOCUS       DAOOBB010000112         6342 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_14400_length_6342_cov_5.072213, whole genome shotgun sequence.
ACCESSION   DAOOBB010000112 DAOOBB010000000
VERSION     DAOOBB010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 6342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6342
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14400_length_6342_cov_5.072213"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..170
                     /locus_tag="PLT31_06095"
     CDS             <1..170
                     /locus_tag="PLT31_06095"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPW94745.1"
                     /translation="LETDAPYLAPVPFRGKPAHSGMIPLIAEHLAQVKNITKEELLSL
                     CRENTRQCYHI"
     gene            complement(167..2158)
                     /locus_tag="PLT31_06100"
     CDS             complement(167..2158)
                     /locus_tag="PLT31_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014544928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPW94746.1"
                     /translation="MRSLQKYFSIRVLLIVICAILAVIAPDVFSLENRYLLKICYSAF
                     FLLTILGAIFYKAPVLSNQLKRRKIRTDIEIPLPDDVATVSRDFQKDLMEKERLFQFM
                     FNSSIDGFWTYDIPTGKVSWSDRAAFILGASQQELGDNFDILKTKVFEKDWSDFRNKF
                     AEALNQSESFSTPLRLLIGKKEITISGRAQCNDEGRPICVFGSLSQPTERTAQFEKQN
                     ALSIYVDALTGVRNRRCFLEKLEKEIQKAKERPDYLFALVLLDIDHFSAINDNYSLDM
                     GDVLLQLVSERISLSCRQNDCVARIGPDVFGIILRNVQNNRTDDELHTIVRRLHSKVK
                     TPLQIGSNEVYVSASMAVVVNREFDNVDDLLANANAMLREIKRSDDRGGIQFFTSGIR
                     EKAMKLYRLEFEIRKAIQCREFLLYYQPVIDIGNENKIVAFEALVRWNNSERGVISPA
                     EFIPMAEETGLIIPLGEMILQMACTQAKKWVDLGYHDIMVAVNFSAKQFAVDNMVEKV
                     KRVLVETNLNPRNLKIEITEYSAMCEVEKTIDIMKALSGMGLQISIDDFGTGYSSLIY
                     LKRYPIHTLKMDKSFVNHVAEDEEDGTFARMVIGIAQSLNLELIAEGVETKAQLEFLR
                     NEGCRLIQGFYFSRPLSEVDALSYLEAHYQKNTTTPVLD"
     gene            complement(2534..3886)
                     /gene="gdhA"
                     /locus_tag="PLT31_06105"
     CDS             complement(2534..3886)
                     /gene="gdhA"
                     /locus_tag="PLT31_06105"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014544927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HPW94747.1"
                     /translation="MALKNEYLQKVYENVVLKNKGENEFHQAVIEFLESLEPVLEKDP
                     GLAKTGILERIVEPERLIQFRVSWVDDAGNVQVNRGYRVQFNSAIGPYKGGIRLHPSV
                     NASILKFLGFEQIFKNSLTSLPMGGGKGGADFDPKGKSDNEIMRFCQSFMTELAKHIG
                     ADTDVPAGDIGTGAREIGYMFGQYKRLRNEFTGVLTGKGLSYGGSLARKEATGYGLLY
                     FTREMLANMKKTDLKGKTVVISGSGNVAIYASEKAQTMGAKVVTASDSNGYVYDPNGI
                     QLDVVKQIKEVERGRISEYAKRVPGSEYHEGCNNIWNVKCDIALPCATQNELDLAGAK
                     ALIANGVMLVAEGANMPSTPEAIEAFLQAGVMFGPAKAANAGGVATSGLEMSQNSERL
                     SWSFEEVEKKLETIMINIFHNAYSASKEYGHEGNLVMGANIAGFKKVADAMKWQGVAY
                     "
     gene            complement(4159..4929)
                     /locus_tag="PLT31_06110"
     CDS             complement(4159..4929)
                     /locus_tag="PLT31_06110"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HPW94748.1"
                     /translation="MKFKMPSSIALELVPRDLNLFLDESRLNLDRYPFIQKINVPEIR
                     SVEIKSFEASKVLLEHSLPAVPHFRLIDRTLDDLLKKLSSLQEMGLKEVLLIGGDPPK
                     NGEFVPSGLTTLEAISQVRKAFPSLKIYAGLDPYRSSFRRELDYAKQKLDAGCNGFYT
                     QPFFSLKVLDLWLEQLSSTELWFGISPVHSDKSRKYWEKVNQVVFPADFSYGVKENID
                     LAKVLLNRIAEVNCNAYFMPITISASDYLKDLFAGDDE"
     gene            complement(5004..5414)
                     /locus_tag="PLT31_06115"
     CDS             complement(5004..5414)
                     /locus_tag="PLT31_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HPW94749.1"
                     /translation="MTCYDPIRQKSVASTPEEAVRQQVICFLKESLGVPPHLMEIEFS
                     LSKIKKASKDRVDILVHDFRRGADISKPWLLVECKAEGQYTWQELEIQLNRYLKVLKP
                     SFIMLGLGVEVRFFAFSEMKNTFLPISELPKYPK"
     gene            5502..>6342
                     /locus_tag="PLT31_06120"
     CDS             5502..>6342
                     /locus_tag="PLT31_06120"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPW94750.1"
                     /translation="MRLKLLSEGAKELSYEIREIVKKANQLQALGLKIHWENIGDPIE
                     KKCQVPQWIKDTVGKIVQENDSYGYCPSQGILTTREFIAKETNKLGGAQITANDILFF
                     NGLGDAIATIYSLLALTTRIIGPSPAYSTHSSAEAAHAHSAPITYRLDPENHWYPDLE
                     ELENKIKYNPTIAGILIINPDNPTGMVYPLEYLEKIVDIAKRYKLFIISDEIYNKIVY
                     NGARAYALAEIIKDVPGIALKGISKEYPWPGSRCGWAEYYNRDKDEQFDAFCKALDNA
                     KMIE"
BASE COUNT         2170 a         1237 c          975 g         1960 t
ORIGIN      
        1 ttcttgaaac agatgctcca taccttgcac cagtgccctt tagaggtaaa cctgcacata
       61 gtggaatgat tcctctaatt gcagaacact tagctcaagt taaaaatatt actaaagagg
      121 aactactttc tctttgccga gaaaatacta ggcagtgcta tcatatttaa tcaagaactg
      181 gcgtggttgt attcttttga taatgcgctt ctaaataact taacgcatcg acttcagata
      241 aaggacgact aaaataaaag ccctgaatta gtcggcagcc ttcatttctt aaaaactcta
      301 attgtgcttt tgtttcaaca ccctctgcaa taagttcaag atttaaagac tgagcaattc
      361 ctataaccat acgtgcaaaa gtgccatctt cttcatcttc tgcgacatga tttacaaaag
      421 atttatccat ttttaacgta tgaataggat aacgtttaag ataaataagc gagctatagc
      481 ctgttccaaa atcgtcaatg gatatttgaa gccccatacc tgataatgct ttcataatat
      541 caattgtttt ttcaacttca cacatcgcac tatattcagt aatttcaatt ttcaaatttc
      601 ttgggtttag attagtctcc acaagaactc gctttacttt ttcgaccata ttatctacag
      661 caaactgttt tgcagagaaa ttaacagcca ccataatatc atgataccct aaatccaccc
      721 actttttggc ttgagtacaa gccatttgta aaatcatttc acctagtgga ataatcaaac
      781 ctgtttcttc tgccatcgga ataaattctg caggagaaat gacacctcgt tcggaattat
      841 tccaacgtac taatgcttca aaagcgacaa ttttattttc gtttccaata tcaatcactg
      901 gctgataata aagaagaaat tcacgacatt gtatggcttt tctaatttca aactctaatc
      961 tatagagttt catcgccttt tctcgaatac cactagtaaa gaattgtatt ccgcctcggt
     1021 catcacttct ttttatttca cgaagcattg cgttcgcgtt agcgagcaaa tcatctacat
     1081 tatcaaactc acgattgaca actactgcca tggaggcact aacataaacc tcattggaac
     1141 caatttgcaa aggcgtttta accttagaat gtaaacgacg cacaatcgta tgcaattcat
     1201 catcagtacg attattctgt acattacgaa gaataattcc aaagacatct ggcccgatac
     1261 gagccacaca atcattctgc ctgcaagata aagaaatgcg ttcagaaacc aactgtaaaa
     1321 gaacatcacc catatctaat gaataattat cattaattgc actgaaatga tctatatcta
     1381 aaagaaccaa agcaaataaa tagtctggtc gttctttagc tttttgaatt tctttttcta
     1441 atttttctaa aaagcaacgt ctatttcgaa ccccagttaa agcatctaca taaatagaaa
     1501 gagcattttg cttttcaaac tgagctgttc gttcggtagg ttggctcaaa gaaccaaaaa
     1561 cacaaatagg tctcccctcg tcattacatt gtgccctacc actgatagtg atttcttttt
     1621 ttccaatcaa aagtcgaaga ggggttgaaa aagattctga ttgatttaaa gcttcagcga
     1681 atttatttcg aaaatcgctc caatcttttt caaaaacttt tgtttttaga atatcaaaat
     1741 tatctcccaa ttcttgctga gaagcaccta aaataaaagc tgctcgatca gaccaagaaa
     1801 cttttcctgt aggaatatca taagtccaaa aaccatcgat agaagaatta aacatgaatt
     1861 gaaaaagacg ttctttttcc attaaatctt tctgaaaatc tcttgaaaca gtagcgacat
     1921 catcaggcaa aggaatttct atatccgttc ttattttacg acgtttcagt tgattagata
     1981 agactggagc cttatagaat atagctccaa gaatcgtcaa caaaaaaaat gcgctataac
     2041 atatcttcaa taagtaacgg ttttcaagcg aaaatacatc aggagcaata acagctaaaa
     2101 tggcacaaat tacgattaat aaaacacgaa tagaaaaata tttttgtaaa gaacgcatag
     2161 aaagttccaa aaaaagcaaa tccttagtat tgggttttcc taatataaac aaataaagaa
     2221 atagtgtact tgtttttctg ttttatagga ttattgtaag tttctttttc agatgtaaga
     2281 aaaattgtaa gtagaaaaac aagtaaacca ataaaaaaaa tgtgttcatc atcacatttt
     2341 atctaaatta aaacgatacc tacaagtgaa gacaatgctt cctcatacac gtgcttagcc
     2401 ctaagaaaaa tacatcaact ctcgccttta tttcagaagc aaaaacctaa ctctctgttt
     2461 ttaagaaata aaaaaaggcc ctttacaaaa aagtaaaaga cctttgaaaa tttgaatgtt
     2521 tattcaaaaa gaattagtac gctacacctt gccatttcat ggcatcagca actttcttaa
     2581 atccagcaat gtttgcgccc atcacaagat tcccttcatg accatattct ttagatgcgg
     2641 aataagcatt gtggaaaata ttaatcatga ttgtttctaa tttcttttcg acttcttcaa
     2701 atgaccagct caagcgttca ctgttttggc tcatttcaag acctgaggta gcaacgccac
     2761 cagcatttgc tgctttagct gggccaaaca tgacacctgc ttgtaagaaa gcttcgatcg
     2821 cttctggtgt agaaggcatg ttagcgcctt cagccacaag catcacacca tttgcaatta
     2881 aagcttttgc accagcaaga tctaattcat tttgtgtagc acaaggaaga gctatatcgc
     2941 actttacatt ccaaatgtta ttacaacctt catgatattc agaaccagga acacgctttg
     3001 catattcaga aatacggcct ctttccactt ctttaatttg tttcacaaca tctaattgaa
     3061 tcccattagg atcatagaca taaccattag aatcagaagc agtcaccact tttgctccca
     3121 tggtttgagc tttttctgat gcataaatgg caacatttcc tgaaccagaa ataacaaccg
     3181 ttttcccttt aaggtcagtc tttttcatgt tggcaagcat ttcacgagtg aaatatagaa
     3241 ggccatatcc agtggcttct ttacgagcaa gtgaaccacc gtatgaaagg cctttaccag
     3301 tcaaaacacc agtaaactca ttgcgaagtc ttttgtattg accaaacata taaccgattt
     3361 cacgagcacc agtaccaata tcaccagcag gaacgtctgt atcagcacca atatgctttg
     3421 caagctctgt catgaaggat tgacaaaagc gcatgatttc attatctgat tttcccttag
     3481 gatcaaaatc agcgccgcct tttccaccac ccataggcaa actagttagg ctgtttttga
     3541 aaatttgttc aaaacctaaa aacttcaaaa tagacgcatt cacagaagga tgtaaacgaa
     3601 taccaccctt atatggacca atcgcagaat taaattgtac tcgataacca cgatttactt
     3661 ggacattacc agcatcatca acccaagaaa ctctaaactg aattagtcgt tctggttcta
     3721 caatacgttc aagaattcca gttttagcaa gaccaggatc tttttctaaa acaggctcta
     3781 atgattctaa gaattctatc acggcttgat gaaattcatt ttcgccctta ttcttcagaa
     3841 caacgttttc ataaactttc tgaaggtatt cgtttttaag tgccattgta tggtctccag
     3901 tttttaaaat tataaaagaa ccgcaggaaa tataactaaa atgagactat aatacttaaa
     3961 gcttaaaaag aaatattacg atttttttct attaaaaaaa aatgcttatt tttataaaaa
     4021 aacaaacatt tttattctgt aaaagggtca aactgggaac gacctaaata aagatcaaca
     4081 ttcaaagggt attttttttg tttacaaaac gcagctataa caattttcct tatgataata
     4141 gcttttcaaa ctgaaatgct attcatcatc acccgcaaaa agatctttta aataatctga
     4201 ggcagaaata gtgattggca taaaataggc attacaattc acctctgcaa tacgattaag
     4261 caacaccttt gctaaatcaa tattctcttt tactccgtag ctaaaatccg ctgggaacac
     4321 cacttgattc accttttccc aatattttcg agatttatct gaatgtacag gactaatccc
     4381 aaaccataat tcagtacttg aaagttgttc taaccaaaga tctaacactt ttaaggagaa
     4441 aaaaggctga gtgtaaaagc cattacagcc agcatctaat ttttgctttg cataatctaa
     4501 ttcacggcga aaagaagatc tatagggatc aagcccagca taaattttta aggatgggaa
     4561 tgcttttcga acttgagaaa tagcctctaa tgtggtaagc cccgatggaa caaattctcc
     4621 gttctttgga ggatcgccac caattaaaag aacttccttt aaacccattt cttgtaatga
     4681 tgataatttt ttcaataaat catctaacgt cctatcaatc aaacgaaagt gaggcactgc
     4741 tggtaaagaa tgttctaaaa gaaccttgga tgcctcgaat gattttattt ctaccgatcg
     4801 aatttctgga acattgattt tttgaataaa aggataacga tctaaattca aacgactttc
     4861 gtctaaaaag agatttaaat ctcttggaac taattctaaa gcaatagaag agggcatttt
     4921 aaatttcata agataaatgt aatatttaat ttgttttagt caatgcataa attcatattt
     4981 ttgcatagat tttgatatta tttttatttt ggatactttg gtaattctga aatgggtaaa
     5041 aaagtattct tcatttcaga aaatgcaaaa aagcgaacct ccactccaag cccaagcatt
     5101 ataaaagaag gtttcaatac tttaagatat cgattcaatt gtatttcaag ttcctgccaa
     5161 gtgtattgcc cttctgcttt acattccact aaaagccagg gttttgaaat atcagctcct
     5221 cttcggaaat catgaaccaa tatatcgact cgatctttag atgctttttt gattttagag
     5281 agactaaatt caatttccat taaatgaggt ggaacaccta aagattcttt taaaaagcaa
     5341 atgacttgct gacgcaccgc ttcttctgga gttgacgcca cgcttttctg acgaatagga
     5401 tcataacaag tcattgcgaa aatataaaat attcaaaata aaaccgcttt caagatcacg
     5461 acttattgtt acatttgaaa catcgtttat aggagtttct gatgagatta aaattactga
     5521 gcgaaggcgc taaagaactt tcatatgaaa tccgtgaaat agtaaaaaaa gcaaatcagc
     5581 tccaagctct aggattaaag atccattggg aaaatattgg cgatccaata gaaaaaaaat
     5641 gccaagttcc acaatggatt aaagatactg tcggtaagat agtacaagaa aacgatagct
     5701 acggttattg tccttctcag ggtattctta ctactcgtga atttatagca aaagaaacca
     5761 acaaattagg cggcgctcaa attaccgcca atgacattct attctttaat ggtcttggcg
     5821 atgcgattgc cactatttat agcttactcg ctttaaccac tcgtattatt gggccttctc
     5881 cagcttatag cacccatagt tctgcagaag cagctcatgc tcattcggct ccaattactt
     5941 atcgactaga cccagaaaat cattggtacc ctgatttaga agagcttgaa aataaaatca
     6001 agtataatcc aacaattgca ggcatattaa ttatcaatcc agataatcca actggaatgg
     6061 tatatcctct tgagtacttg gaaaaaattg tagatatcgc caaacgttac aaactcttta
     6121 ttatttccga tgaaatttat aataagattg tttataatgg tgctcgtgct tatgctctag
     6181 ctgaaatcat taaagatgtt cctggtattg cattaaaagg aatttcaaaa gaataccctt
     6241 ggccaggatc tcgttgtggt tgggcagaat attataatcg tgataaagat gaacaatttg
     6301 atgcattctg caaagcttta gataatgcta aaatgatcga ag
//
LOCUS       DAOOBB010000113         6233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_14697_length_6233_cov_4.921819, whole genome shotgun sequence.
ACCESSION   DAOOBB010000113 DAOOBB010000000
VERSION     DAOOBB010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 6233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6233
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14697_length_6233_cov_4.921819"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1452
                     /locus_tag="PLT31_06125"
     CDS             <1..1452
                     /locus_tag="PLT31_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94751.1"
                     /translation="LKGNTTWDLVQGSTYPFIETLYNAFESAYLQEIPNYFEKTFFSL
                     NFYGKWDAHELNTKSTSFIPTTSSLDLLCDGKISTTVKCAHSQTESGLSFESPGNSST
                     AKAFYAVDKTHPRYKESYSGRHIEPPYNLDGTPKTNVIQGIQTDLWRLMCELAKIDYN
                     QENNTFDNPYFIGQFNPKTSCLDQGKIPEAILNAGRIQHETPHFARYDYAPEQNEMTE
                     NVSFFVPARWQKVASFDYGKSLPAGGLFEVVLKMDNPKSNWLKAEILLNQVKDGSHQI
                     QLEEKSISPDGLYHTIRWQFPTSPGALQKFRWIRLVLNSSGGDVTISKIRFIKNTVSD
                     SPQPSPIPFSNIYPGNYTFYTPWNTNAPHDLNYKNSTREGARFYFEKAYDGVQWSFKK
                     SYSFNPYTKIRVSYSPNTCLNSFLYIDNPTKSRSFLKNGTLNGLFMNKVFSIEDLIPT
                     ELNTSDILIGSKLVLQSSRANEECIIHSISLER"
     gene            1748..2518
                     /locus_tag="PLT31_06130"
     CDS             1748..2518
                     /locus_tag="PLT31_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94752.1"
                     /translation="MKSFRYLFPCFLLITISIFLSGCDTVTLHALGSNPIPVMHEPRT
                     EKTDSSEMVLSANLNGSILTTGINIKEYSSIGGHLDVTYRLGNLFSPLFFNGTVFGSY
                     GKLKLTCDDSDCNENFRSLTEEEKSYSTASIQERIRVGVELMPFFLTIGASAGLHFYQ
                     DFLQYEDLRKKLKETNYVNGNYSYDVYPEIRVWLGSRLGSKGKYGTINFEGSYQLYAD
                     SYDNILSVSLGYFHPSGWHGGVIIHNSMILTAGKSFKF"
     gene            complement(2716..3069)
                     /gene="rplT"
                     /locus_tag="PLT31_06135"
     CDS             complement(2716..3069)
                     /gene="rplT"
                     /locus_tag="PLT31_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPW94753.1"
                     /translation="MPRSKSRVPSRERRKKILKAAKGFYGRRKSNIRIAIDAVAHAGQ
                     YAYAHRRDKKGDFRTLWITRLNAAVREHGVSYSQFIFLLGKANVKMNRKVLADMAVAD
                     PAAFAEVVKLVKSVA"
     gene            complement(3087..3284)
                     /gene="rpmI"
                     /locus_tag="PLT31_06140"
     CDS             complement(3087..3284)
                     /gene="rpmI"
                     /locus_tag="PLT31_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HPW94754.1"
                     /translation="MPKMKTHSGAKKRFRVTGSGHVKFKHAGMRHILTKKSTKRKRNL
                     RQAGAIKKCDIYHVKRLLVLA"
     gene            complement(3306..3902)
                     /gene="infC"
                     /locus_tag="PLT31_06145"
     CDS             complement(3306..3902)
                     /gene="infC"
                     /locus_tag="PLT31_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPW94755.1"
                     /translation="MLQNARSRQMPNRPSDGTRINEDIRISPIRLVKDDGEQLVIETH
                     KALQMAKEAELDLVEVSPNAKPPVCRIMNYGKFRFEQMKRAKAAKAKQHIVKVKEIKL
                     HPKTALNDYQYRMEQAKDFLKSGMKVRLVMQFRGREMAHMDYGRRLMDQARIDLAESG
                     DLEMDARVEGNTMASIYTPKRGVTPAKPVTEPMAAGEA"
     gene            4029..4100
                     /locus_tag="PLT31_06150"
     tRNA            4029..4100
                     /locus_tag="PLT31_06150"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4061..4063,aa:Gly,seq:tcc)
     gene            4426..4977
                     /locus_tag="PLT31_06155"
     CDS             4426..4977
                     /locus_tag="PLT31_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94756.1"
                     /translation="MGMGFLKNKTSIGILCLLFSMPLQAADLLAFPGAEGFGRFTVGA
                     RAVSSPEIYHVTNLNDSGVGSFRDAVSKAGRIVVFDVSGIINLKSTLIFSGNSIIAGQ
                     TAPGDGIMLYGDRVSFSGANNLIVRYLRIRMGINGSSGKDAAGVANGKNMIFDHVSVT
                     WGRDETFSISWDSKGTEPSNITI"
     gene            5026..>5909
                     /locus_tag="PLT31_06160"
     CDS             5026..>5909
                     /locus_tag="PLT31_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94757.1"
                     /translation="MQTDGGVTLFRNLYIDNKTRNPKVKGLNQFVNNVIYNWGGGGGY
                     IMGGSAGDSWASIEDNYFIKGPSTGGTEAFVRATETFQVFPKNNYLDYSLDGVLNGTI
                     ADESVYSGATIVSSYSGFSNSPQLHPTITAQTSAAEAYKITVEDVGASYPARDEVDTY
                     LINELKSLGSKGALISNESALGLSGNVGTLASGTAPTDTDKDGIPDSWEDQMGLNKNN
                     ASDAITKDASGYLYIEKYINGLVEKTTNPSLSAKGSSSQTIALGKTISSITYTFANCT
                     GAQVTGLPTGVTSNLNAA"
     assembly_gap    5910..5919
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5920..>6233
                     /locus_tag="PLT31_06165"
     CDS             <5920..>6233
                     /locus_tag="PLT31_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94758.1"
                     /translation="SELGTFQFTVTTSGGEGNAATLSGSITVTKAVPVVTPVAILSNL
                     NAANPVEGVGVYEEKNTGYVDNGYYNFSNLIESYGLWKISSNTSGEAILSLRFANAGT
                     TS"
BASE COUNT         1900 a         1171 c         1137 g         2015 t
ORIGIN      
        1 ctgaaaggta ataccacttg ggacctagtt caagggagca cgtacccatt tatagaaact
       61 ctatataacg cttttgaaag cgcctattta caagagattc ctaattattt cgagaaaact
      121 tttttttctc tcaattttta tggaaagtgg gatgctcatg aattaaacac taaaagtacg
      181 agttttattc ctaccacgag ctctcttgat ttactatgtg atgggaaaat ttccactaca
      241 gtgaaatgcg ctcattctca aacagaaagt ggtttatcct tcgagtctcc tgggaattcg
      301 tctacagcaa aggctttcta cgcagtagat aaaacacacc ctcgttataa agaaagttat
      361 agtggtcgtc atattgagcc accttataat ttagatggaa ccccaaaaac aaacgtaatt
      421 caaggtattc aaactgattt atggcgatta atgtgtgaac ttgcaaaaat cgattataac
      481 caagaaaata atactttcga caatccttat tttattggtc aatttaatcc aaaaacgagt
      541 tgcctagatc aaggtaaaat ccctgaagcc atattgaatg ctggtcgcat tcaacatgaa
      601 acgcctcatt ttgctcgtta tgactatgct ccagaacaaa atgaaatgac agagaatgtt
      661 tccttttttg ttcctgcacg gtggcaaaaa gtcgcttctt ttgattatgg gaaaagtctg
      721 cctgcaggcg ggctatttga agttgttctt aaaatggata atcctaagtc taattggctc
      781 aaagcagaga ttctcttgaa ccaagtgaaa gacggctctc atcaaattca actagaagaa
      841 aaatctattt ctcctgatgg attatatcac accattcgat ggcaatttcc aacttcacca
      901 ggcgcacttc aaaaattcag atggatcaga cttgtactaa actcttctgg tggagatgtc
      961 accatttcta aaatccgatt tattaaaaat actgtttctg attcacctca accatcgcct
     1021 attccttttt ctaatattta tcctggaaac tatactttct atactccatg gaatacgaac
     1081 gctcctcatg atttaaatta taagaattct accagagagg gcgctcgctt ttattttgaa
     1141 aaagcctatg atggagttca atggtctttt aaaaagtcct attcattcaa tccatatact
     1201 aaaattcgcg tctcttactc accaaatact tgcttaaatt ctttcttgta cattgataat
     1261 cctacaaagt ctagatcctt cttaaaaaat ggaaccttaa atgggttatt catgaacaag
     1321 gtgttctcca tagaagactt aattcctact gaattaaata cgtctgatat tctaataggt
     1381 agtaaactgg tattacaatc ttcaagagct aatgaggaat gtattattca tagtatttca
     1441 ctagaacgtt aaacagaata aatctatagt atctgtaaca acgatttcaa aatatgattt
     1501 cgttgttatt aatttattat acaccttatt taatataaaa gcctacacaa ttgaagagct
     1561 atacaagatt atattatttt ttatatcgat atttaaaaaa gcggagcaag acttttaagg
     1621 taaaaaactt gaaagtttta tatgaggatc aaacaaataa aattaattta tacacaaaat
     1681 atatgtgtat atagctataa tataatttat attattcgca tcataaaatt tattaaggag
     1741 agtaatgatg aaatcattta gatatttatt tccatgtttt ttattaatca caatttctat
     1801 ttttttaagt ggttgcgaca cagtgacact gcatgcttta ggaagcaatc caattcctgt
     1861 aatgcacgaa cctagaactg aaaaaacgga ttccagcgaa atggttctta gtgcaaactt
     1921 aaacggttct attttaacta ctggaataaa catcaaagaa tattcttcta tcggaggtca
     1981 tcttgatgtc acttatcgtt taggaaatct tttttctcca ctattcttta acggaactgt
     2041 atttggttcg tatgggaaat taaaacttac ttgtgatgat tctgattgta atgaaaattt
     2101 ccgttctctt acagaagaag agaaatccta ttcaacggct tccattcaag aacgcattag
     2161 agttggcgtt gaattaatgc catttttcct caccattgga gcaagtgcag gccttcattt
     2221 ttatcaagat tttttacaat atgaagatct aagaaaaaaa cttaaagaga ctaactatgt
     2281 taatggtaat tattcttacg acgtatatcc tgagattcgc gtgtggcttg gttcaagact
     2341 tgggtcaaaa ggtaaatatg gcacaatcaa ttttgaaggc tcataccaat tatatgctga
     2401 tagctacgat aatattcttt ctgtcagctt aggttacttt cacccaagtg gctggcacgg
     2461 aggcgtaata attcataact ctatgattct tactgcagga aaaagcttta agttttaaaa
     2521 ttaagattta agaaaagagt ctttaattaa atacaattag ggactctttt tttgatgtta
     2581 ctaaatccaa aatgtttaag gcaatcattg agttaaatgt tttagaaaat gaatagaaac
     2641 cataaatatt ctttttctca tttattataa aaaaaggctc ttctaacgaa gagccttttt
     2701 cttaatctaa aaagattaag ccacagactt cactaactta acaacctcag caaaagctgc
     2761 tgggtcagca acagccatgt cagctaaaac ctttcggttc attttgacat tagccttacc
     2821 tagtaagaaa atgaattggc tataactaac gccatgttca cgaacagcgg cattcaagcg
     2881 agtaatccac aatgtgcgga aatcaccttt cttgtcacga cggtgagcat aggcatattg
     2941 gccagcatgg gctacagcat caatcgcaat acgaatattg gacttacgac gaccgtagaa
     3001 accttttgca gccttaagta tttttttacg acgttcacgg gaaggaactc tggactttga
     3061 gcgtggcata ttttaactct ccttgattaa gccaatacaa gcaaacgctt gacatggtag
     3121 atatcacatt ttttgattgc accagcttga cgtagattac gtttacgctt tgtgctcttt
     3181 ttcgttaaaa tatggcgcat gccagcatgc ttaaacttga catggcctga accagtcacg
     3241 cgaaagcgct tctttgcacc gctgtgtgtt ttcattttag gcatttgtac ctcgtaggtt
     3301 gtatgttaag cctcacctgc tgccatgggc tcggttacgg gctttgcggg tgttaccccg
     3361 cgttttggag tgtagataga agccattgta ttgccttcta cccgcgcatc catttccaag
     3421 tcgccacttt cggccaagtc tattctagct tgatccatga gtctgcgacc gtaatccatg
     3481 tgcgccattt cacgcccacg gaattgcatc actaaacgta ctttcattcc gctttttagg
     3541 aagtcttttg cttgctccat gcgatactga tagtcattaa gagctgtttt tggatgcagt
     3601 ttaatttctt tcacttttac aatatgttgt ttggcttttg cagccttggc tcttttcatt
     3661 tgttcaaaac gaaatttgcc atagttcata atgcgacaaa caggcggttt tgcattcgga
     3721 gatacttcca ccaggtctag ttcggcttct ttagccatct gaagagcctt gtgtgtttct
     3781 atcacaagct gttctccatc atctttaacg agacgaattg gggaaatacg gatatcttca
     3841 tttatccttg tcccatcgct tgggcgatta ggcatctgac gactgcgagc gttctgtaac
     3901 aagttatgca acctcttgat ttgtttgtaa aaggatagaa aatttaataa aattcaaaaa
     3961 aaatcacaat atgatttgaa aatacctttg taaataatcg tttttttgta tatttgactt
     4021 caaagattgc ggcggtagct caatggtaga gctttagcct tccaagctaa tggttgccgg
     4081 ttcgatcccg gtctgccgct cttaacagaa aagcttcctt tatataggaa gtttttctgt
     4141 tttaaactcc ttttttggtg cttttgaata ggaaaaggta tttaatcata caatagtgcc
     4201 gttccttatt aagcgttgtc tataaacaat gctttcttct gtttttctct ctctttcgat
     4261 accattatcc tattttaata aaatggaacc tctaaaaatt ggtttgcttc cacaaaattg
     4321 agcaaggccg tgccacagcc aattttgaag aaaatgattt tttgaggtgc tcaaaaggca
     4381 ctcatgactt ttgagtggtg ggtctccatt tgtaaaggag tttttatggg aatgggtttc
     4441 ttaaaaaaca aaacatcaat cggtattctt tgtttattat tttcaatgcc tctccaagcg
     4501 gctgatttgc tcgcttttcc aggtgcagaa ggctttggac gtttcacagt tggagcaaga
     4561 gccgtctctt ctcctgaaat ttatcacgtg actaatttga atgattctgg agtgggatcc
     4621 tttcgcgatg ccgttagtaa agcaggacga attgttgttt ttgacgtttc tggaattatc
     4681 aatttaaaat ccacattaat atttagcgga aactctatta ttgcaggcca aactgctcct
     4741 ggtgatggca ttatgcttta tggagacaga gtttcttttt ctggtgctaa taacttgatc
     4801 gttcgatacc ttcgaattcg catgggaatc aacgggtcct ctggtaaaga tgctgcaggt
     4861 gtagcgaatg gcaaaaatat gattttcgat catgtctcag tcacttgggg aagagatgaa
     4921 actttttcga ttagctggga tagtaaggga acggaacctt ctaatatcac catctaaaat
     4981 tccattattg gacaaggact tcaaacacat tcctgtggtg gtttaatgca aaccgatggc
     5041 ggtgttacat tattcagaaa cttatatata gataataaaa caagaaatcc taaagtcaaa
     5101 ggtctaaatc aatttgtaaa caacgtcatt tataactggg gcggtggcgg cggttatatt
     5161 atgggtggat cagcaggcga ttcttgggca agtattgaag acaactactt tattaagggc
     5221 ccttccacag gtggtacaga agcctttgtt agagccacgg aaacattcca agtatttcca
     5281 aaaaacaatt atttagacta tagtttagat ggtgttttga atggtactat cgcagatgaa
     5341 tccgtctata gtggagccac cattgtttct tcttatagtg gattttctaa ttctcctcaa
     5401 ttacacccaa caatcacagc tcaaacttct gccgctgaag catacaaaat cacagtagaa
     5461 gacgttggtg cttcttatcc agctagagat gaagtagaca cttacctcat taacgaatta
     5521 aaatctcttg ggtctaaagg agctttgatt tcgaatgaat ctgcactagg cctttcagga
     5581 aatgttggaa ctcttgcttc tggaacggct cccactgata ccgataaaga tggtattcct
     5641 gattcttggg aagaccaaat gggattaaat aaaaataatg cctccgatgc cattacaaag
     5701 gacgcttctg gatatcttta tattgaaaaa tatatcaacg gtctagtgga aaaaacaact
     5761 aaccccagtt tatcagccaa aggatcttca tctcaaacca ttgctcttgg aaaaacgatt
     5821 agcagtataa cttatacttt tgcgaattgt actggcgctc aagttacagg ccttcctaca
     5881 ggagttacta gtaacttaaa tgcggctcan nnnnnnnnnt ctgaattagg cacattccaa
     5941 ttcaccgtga ccacaagtgg aggcgaaggg aatgcagcaa ccctttctgg gagtatcact
     6001 gtaacaaaag cagtacccgt tgttactcca gtagcgattc tttcaaatct taatgcagca
     6061 aatccagtag aaggagtcgg tgtctatgaa gaaaaaaata ctgggtatgt agataatgga
     6121 tattataatt tttctaatct catagaaagc tacggtttat ggaaaatttc ttcaaacaca
     6181 agtggagaag ccattttatc acttcgtttc gcaaatgctg gtactacttc aag
//
LOCUS       DAOOBB010000114         6210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_14743_length_6210_cov_5.085784, whole genome shotgun sequence.
ACCESSION   DAOOBB010000114 DAOOBB010000000
VERSION     DAOOBB010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 6210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6210
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14743_length_6210_cov_5.085784"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..850)
                     /locus_tag="PLT31_06170"
     CDS             complement(179..850)
                     /locus_tag="PLT31_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94759.1"
                     /translation="MIRNRITELREQVEVIRKSRRMPYWKLSYADFMLQRVEEYLLVD
                     RTPEAVLMCDRLDSWVSKNKPSKQWHEEIHNPKIVFWSAPFLRKVIAELKTTLNQKKQ
                     LIPAPERDSFSRRLDKIESWIEEGKILKAREDLLAVRLSLIARLKRSYRARTLTFAPQ
                     SKNSSISRASSTVVGLYNSQHTLENTFSLIGERDPIWVEDFLEVYNNLFKYVERLAVK
                     DKKKK"
     gene            complement(847..1485)
                     /locus_tag="PLT31_06175"
     CDS             complement(847..1485)
                     /locus_tag="PLT31_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPW94760.1"
                     /translation="MRKSKSEENASWFVFTDLTVGLLALFILAFVAMATLKEQKAEDL
                     TRTEEEVLSCQEEMRKIAMERNALLSSSLKSTIEKGLIALEDGNIQIQASFLFPLNGA
                     ELTPEGTQVIQAIGKGLLDILDTSDVIMVSGFTDDTPIKSSTYTNWNLSAERAINVVK
                     TLIAQGFPADRLFAAGFGEYHPKYPNTSDENRRLNRRVEIGVTPIRKSENKH"
     gene            complement(1485..3065)
                     /locus_tag="PLT31_06180"
     CDS             complement(1485..3065)
                     /locus_tag="PLT31_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94761.1"
                     /translation="MKNNSKLILLLTVLGFTVLLASAFYFGAPLFGWIIVVAIFAIFL
                     GSQWRDYKLMQMIETERNNLKVHSNGLLPDKVQPGMVGERISVVKRLALRKVPFSSQL
                     VTEILDAKASVQVGRSMGGVVVLLGLLGTFFGLMLAVSTAGSALDNSTTENTLITVQS
                     IFSSMKGIFGTSLCGLFSALVLNASHALVEKENSDFVSDLDKFTLLELIPTFSVQEKS
                     SDSHVHELMAALAENMQKNQEATLASISETFISLQKESVQSITQVTQNQSKSLEEFLS
                     QMQKKWSSMEHFASDEIKKSIQQMASTFAETISGQVNALKEQSNAFIQSLKEESLRSS
                     QSFASSMEATQAKQLEGVEHLRSIASRVIEKTEVDASAFSSSIQKEFGALAEKIGVEF
                     SQLTESSSALAEVQKRLVTDVESRTTKELEAADVLANNISEAATLMRVNQSEFAANLE
                     VLRRGLETVIEKLSGDTAEHEDENNFMEQLQASLQAFHERASEVLMENAVKTQEILLE
                     VLEQAQRVSAASNVKSEG"
     gene            complement(3076..3990)
                     /locus_tag="PLT31_06185"
     CDS             complement(3076..3990)
                     /locus_tag="PLT31_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94762.1"
                     /translation="MDESFRRAIRKMTGASIVRFGSRPNRASMIANLSQVMNATWSIA
                     LFEHLDANDNDPSATMAGPETISFSSSAWKLWESEFPHLIDEFTSKFEEFHFKWNQRF
                     STDEIHRQLLQSQWIAQDTQGWYFSSKSTKQEIKNLYFSTIHLMVGDAEKLLVMMSSR
                     ASHLQERLSNFWYKEGATDAISKVLPCLEASLRSQETEMISSLRASLAEVALKRFYAG
                     FSTGSKTRYYPTSMSCARNVGRQWDLNGAYENPFLAYTDDFCDYAAGLTLLIGRWYQE
                     KWSLFLRGFSRGQLDLFGQNSQSFNTNP"
     gene            complement(4018..4719)
                     /locus_tag="PLT31_06190"
     CDS             complement(4018..4719)
                     /locus_tag="PLT31_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94763.1"
                     /translation="MKKSLFFFSVLFSLTWAEVPFPVIESGKVVLTEKNSPYLLETSV
                     VFSGTDTLIIEPGVQVFMMPYAKLLLRGPVQILGTEKKPVVFKSLDTTESWSGVHFVS
                     NTAPFYVKNLVVENAFRNSVTQSGGVFESVSFINNYYGLWVNSSNQVSLKKCSFTRNR
                     FALSVGAGSIKAESTQIFGNVFGLYIEKGGEYSGNLQLIKGNLEADIRKETEELAGKG
                     KTVSRSVWHRIESSF"
     gene            complement(4716..5534)
                     /locus_tag="PLT31_06195"
     CDS             complement(4716..5534)
                     /locus_tag="PLT31_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94764.1"
                     /translation="MKKTLLLASFLLLACDQQTTNLVFNTQNIADQKFLMQADMNVFL
                     DDSMPQDTIESMETSLRLIVTSSLLMAYDDSSARFEIKVDSVEYTSDKRSVEEFRYVE
                     QYLSSQNFQFKMAKDGLMSEPVMDNYVAHPEGDELEIAKLFLKIQPVFPGKPVRVGES
                     WERQHAVTGENNEQMVIYKSFTLEDLFNRSGVLLAKIRMNMRYKQVSNNPQIKMTSED
                     FIVGSGFLLFNVTMGVVEESVLEVNGKVNVSDKYSEEEIPKLRVIQKLKLRSFV"
     gene            complement(5586..6083)
                     /gene="tadA"
                     /locus_tag="PLT31_06200"
     CDS             complement(5586..6083)
                     /gene="tadA"
                     /locus_tag="PLT31_06200"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HPW94765.1"
                     /translation="MDEIQQREDEKFMRLALRQAEMAFDAKEVPIGCVIVKEGKVIGK
                     GYNQVESLKDATAHAEILAIGAASSAVENWRLSDCTLYVTLEPCPMCAGAILNSRISR
                     VVYGSPDSRFGGCGTTVDVISNNALKRDVVITSGVLAEECLGLLQLFFQEMRARKGDT
                     GTKPL"
BASE COUNT         1968 a         1378 c         1063 g         1801 t
ORIGIN      
        1 acgatgtgac gttcgtaagg ctaatgaatc attaacaatg ccaacaaccc accctacatt
       61 gcgttcactg tcccaccact cctctgtacc ggagcaagaa atcaaaaaag agctactagt
      121 gtagtgtata aatagagtct catatagaat cctcgccccc aaccccctaa tcaatgactt
      181 acttcttttt tttgtctttg accgctaagc gttccacata tttaaacaga ttattataga
      241 cttctaaaaa gtcctctacc caaattgggt cgcgctcccc aatcaacgag aatgtatttt
      301 caagcgtatg ctgagagtta taaagcccca ccacagttga agacgctctc gagatcgaac
      361 tatttttcga ctgaggcgca aacgtcaaag ttctcgccct atatgatcgc tttaatcgtg
      421 cgatcaaact caaacgaacc gcgagcaaat cttctcgagc ttttaaaatt ttcccttctt
      481 caatccaaga ctcaatctta tctaaacgac gagaaaaaga atcccgttct ggagcaggaa
      541 tcaactgttt cttttgattt aaagtcgtct ttaattctgc aatgactttt cttaaaaaag
      601 gagcagacca aaaaacaatt tttggattat ggatttcttc atgccattgt tttgagggct
      661 tattctttga aacccaagag tctaagcgat cacacatcaa aacggcttct ggtgttcgat
      721 ctactaaaag atattcttct actcgctgca acataaaatc agcataagaa agcttccaat
      781 aaggcatccg acgtgatttt cgaatgacct ctacttgttc tcgaagctct gttatacgat
      841 ttcgaatcaa tgcttgttct ccgatttacg aataggagtt acacctattt ctactcttcg
      901 atttaagcgc ctattttcat ctgatgtatt tgggtatttg gggtgatatt ctccaaagcc
      961 cgcggcaaaa agacgatctg ctgggaaccc ttgagcaatc aacgttttga ccacattaat
     1021 agcacgttcc gcagataagt tccagtttgt ataagtggat gatttaatag gtgtatcgtc
     1081 tgtaaaacca gagaccataa tcacatcact tgtatccaag atatctaata agccttttcc
     1141 aatcgcttga atcacctgag tgccttctgg agtgagttct gccccattca aagggaataa
     1201 aaaggatgcc tggatttgaa tattaccatc ttctaaagcg attaagcctt tttcaattgt
     1261 actttttaag ctactagaga gcaatgcgtt acgttccatt gcaatttttc gcatttcttc
     1321 ttgacaactt aaaacttctt cttctgttcg agttaagtct tcggcttttt gttctttaag
     1381 agtggccatc gctacaaaag ctaaaataaa aagagctagt aaaccgacag tcaaatccgt
     1441 aaagacaaac caggatgcat tttcctcgct tttgctttta cgcattatcc ctccgatttc
     1501 acattagaag cagcagaaac acgctgagct tgttctaaaa cttctaataa aatttcttgg
     1561 gtcttcaccg cattttccat taacacttcg ctagctcttt catggaaggc ctgtaaagag
     1621 gcttgcaatt gttccatgaa attattttca tcttcatgtt ctgccgtatc gccagaaagc
     1681 ttttcaataa ccgtttctaa accacgacga agcacttcta agttagccgc aaattccgat
     1741 tgattgacgc gcattaatgt ggctgcttca gaaatattat tcgcaagcac atcggcggct
     1801 tctaattcct tagtcgttct agattctaca tcggtgacta agcgtttttg aacttcagct
     1861 aaagcagaag aagattctgt caactgacta aattccacac caatcttttc ggcaagcgca
     1921 ccaaattctt tttgaatcga agaagaaaat gcagaggcat ccacttctgt cttttcaata
     1981 acacgagatg cgatcgaacg taaatgttcc acaccctcca gctgttttgc ctgagtggct
     2041 tccatagaag aagcaaatga ttgagaagaa cgaagagact cttctttaag agactgtata
     2101 aatgcattcg attgttcttt taatgcattc acctgaccag aaatagtctc tgcaaaagta
     2161 ctagccattt gctgaataga ttttttaatt tcatcagaag caaagtgctc catcgaggac
     2221 catttttttt gcatctgact taaaaattct tctaacgatt ttgattgatt ctgagtcact
     2281 tgagtgatag attgtaccga ttctttttgc aaagaaataa acgtctctga aatagaagcg
     2341 agtgtagcct cttgattctt ttgcatattt tcagctaaag cagccatcaa ttcgtgtaca
     2401 tgagagtcag aagatttttc ttgtacgcta aatgtcggaa tcaattcaag caaagtaaac
     2461 ttgtctaaat cagaaacaaa atctgaattt tctttttcaa caagagcatg agacgcattc
     2521 aaaactaaag cagagaacaa gccacacaag ctagttccaa aaataccctt catactagag
     2581 aaaatcgact gcactgtaat caaagtattt tctgtagtgg aattatcgag agcagaacca
     2641 gcagtcgata cagcaagcat taaaccaaag aaggtaccta aaaggcctag taaaacaacc
     2701 actccgccca tgctgcgtcc aacttgaacg gaagccttcg catctaaaat ctcagtcact
     2761 aactgagaag aaaaaggcac tttacggagc gcaagtcttt tcacaacgga aatacgttcg
     2821 cccaccatgc ctggctgcac tttatcagga agcaatccat tggaatgcac ttttagatta
     2881 tttcgttccg tttcgatcat ttgcatcaat ttgtaatctc gccattgaga accgaggaaa
     2941 atggcaaaaa tagctacgac aataatccaa ccgaataatg gtgctccaaa ataaaaagca
     3001 ctggcaagaa gaactgtaaa gcctaaaacg gtcaataaaa ggattagctt ggaattattt
     3061 ttcatccgtt tcatcctaag ggttagtgtt aaaagactgc gaattctggc cgaacaaatc
     3121 caattgccct cgcgaaaatc cacgtaaaaa aagcgaccac ttctcttgat accatctgcc
     3181 gatcagtaaa gtaagacctg ccgcataatc acaaaaatca tctgtataag ctaagaatgg
     3241 gttttcataa gcaccattga gatcccactg gcggcccaca tttcttgcac aactcatcga
     3301 agtcgggtaa taacgcgttt tagatcctgt actaaaaccc gcatagaaac gcttcaaagc
     3361 gacctctgcc aaagaggctc ttaagcttga aatcatttcc gtttcttgac tgcgaagaga
     3421 ggcttctaaa caaggaagaa cttttgatat agcatctgtt gcgccttctt tataccaaaa
     3481 attactcaaa cgttcctgta aatgagatgc acgagaagac atcatcacta aaagcttttc
     3541 agcgtctccg accattaaat ggatcgtaga aaaataaaga tttttaattt cttgttttgt
     3601 ggatttagaa gaaaagtacc aaccttgggt atcttgtgca atccactgac tttgaagtaa
     3661 ctgcctatga atctcatctg tagaaaagcg ttgattccat ttaaaatgaa attcttcaaa
     3721 tttagaggta aattcatcga tcaaatgcgg gaactccgat tcccataatt tccaggcaga
     3781 agagctaaaa gaaatagttt ctggaccagc cattgtggct gaaggatcat tatcattagc
     3841 gtctagatgt tcaaatagag caatagacca agtcgcattc atcacttgag acaaattggc
     3901 aatcatcgaa gcccgattag gccgcgaccc aaaacgcaca atacttgcgc ctgtcatttt
     3961 tcgaattgct ctgcgaaatg attcgtccac gttgaaaata taccaaatcc acaaaaatta
     4021 aaagctggat tctatccgat gccatacgga tcttgagaca gtctttccct ttccagctaa
     4081 ttcttcagtt tctttacgaa tatcggcttc taaattgcct ttaattaatt gcaagttccc
     4141 cgaatactct ccaccttttt cgatatataa gccaaaaaca ttcccaaaaa tctgagtcga
     4201 ttcagccttt atggaacctg cacccacgga tagagcaaaa cgattacgcg taaaagagca
     4261 cttttttaag gagacttgat tagaagaatt cacccataag ccataataat tattaataaa
     4321 agacaccgat tcaaaaacgc caccagattg agtcactgaa tttctaaagg cattttctac
     4381 cactagattt tttacgtaaa aaggcgccgt attagacaca aaatgaaccc cactccagct
     4441 ttctgtggta tctaaacttt taaaaacaac aggctttttc tcagtgccca atatttgcac
     4501 tgggccgcgc aaaaggagct tcgcataagg catcataaag acttgaacac caggttcaat
     4561 aatcaaagta tctgtaccag aaaagacaac actagtctct aacaagtagg gactattttt
     4621 ttcagtaaga actaccttcc ctgattcaat cacaggaaaa ggaacttctg cccatgttaa
     4681 tgagaaaagg acactgaaaa aaaacagtga ttttttcaca caaagctcct taatttcagc
     4741 ttctggatca cacgtaattt aggaatttct tcttctgaat atttatcaga aacattgact
     4801 tttccattca cttctaaaac ggattcttct actacaccca tagtgacgtt aaaaagaaga
     4861 aacccgctac ccacaataaa atcttcacta gtcattttta tttgaggatt attacttact
     4921 tgtttgtatc gcatattcat acgaattttt gcaagtaaaa cgccactgcg attaaataga
     4981 tcttctaaag taaagctttt ataaatcacc atttgctcat tattttctcc agtgacagcg
     5041 tgctggcgtt cccaagattc acctacgcga acaggcttgc caggaaaaac aggctggatt
     5101 tttagaaata acttagcgat ttctaattca tcgccttctg gatgagccac ataattatcc
     5161 atcactggtt cactcattaa accgtcttta gccattttaa actggaaatt ttgcgagctt
     5221 aaatattgtt ccacataacg gaattcttcg acagagcgct tatctgaggt atattctaca
     5281 gaatctactt tgatttcaaa acgagccgaa ctatcatcgt aagccatcaa taaagaagaa
     5341 gtaacaatca gccgaagaga ggtttccatc gattcaatcg tatcctgagg catagaatca
     5401 tcgagaaaga cgttcatgtc tgcttgcatc aagaattttt gatctgcgat gttttgagta
     5461 ttaaagacta aattggtcgt ttgttgatca caagccaata ataaaaagct ggctaaaagc
     5521 aaagttttct tcatgcccct aaaaataaat ccgaaaaggg aaaaacgtta ctgaaaacta
     5581 atgttttaca agggtttcgt accagtatca cccttacggg cacgcatttc ctggaaaaag
     5641 agttgtagaa gacctaaaca ctcctccgca aggaccccag aagttataac gacatctcgc
     5701 ttgagagcgt tatttgaaat gacatcaacc gtagtgccac agccaccaaa gcgggaatct
     5761 ggagaaccat aaacgactct tgaaatgcgg ctattcaaga ttgcccctgc acacattggg
     5821 caaggttcta atgttacata aagcgtacaa tcacttaaac gccaattttc tactgccgaa
     5881 gaagcggctc caatcgctaa aatctctgca tgagccgtgg catcttttaa cgattctacc
     5941 tgattatagc cttttccaat gactttgcct tctttcacaa tcacacagcc aataggcact
     6001 tctttcgcat caaaagccat ttccgcttgg cgtaaagcca aacgcatgaa cttttcatct
     6061 tctctttgtt gaatttcatc cattttctaa aaatagaaaa gacttaaggg gcattcaata
     6121 ttctcacgtc ttcgggtgcc ataattcact aaaatcctta aaaaccaaaa atcccgctca
     6181 tcgcgggact ttatcattgc ggccatcgct
//
LOCUS       DAOOBB010000115         6204 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_14758_length_6204_cov_4.316637, whole genome shotgun sequence.
ACCESSION   DAOOBB010000115 DAOOBB010000000
VERSION     DAOOBB010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 6204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6204
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14758_length_6204_cov_4.316637"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..2249
                     /locus_tag="PLT31_06205"
     CDS             111..2249
                     /locus_tag="PLT31_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HPW94766.1"
                     /translation="MMNLKKTAFLVTFTVLVFFGFSTADNPISNYHYLADPTAFSTDS
                     TFYIVTDTDDECPSTGENDYKIRALYAFSSKDMKNWTDHGMVLRYNREVSYISNIWAS
                     GAVAHDGVFYIIYPNGASGVGIVSSKDIAGPYIDPVGKLLVGGGGVTDCGGISWCFDP
                     AIFVDDDGKSYMTYGGGSSGSRPYGDNFDLFQFSKLSHDQVTLDVNSRTRISGANKSF
                     EASYIHKRNGTYYFSYNDQSQAISYATSKNIKGPYTYQGVVINNPSSINGKGGNNHHG
                     FAQFKDKWYAVYHDRRLVMDPEHPAAGGQINGLTTGNHRSTSIDEVTWNGDKMNVVKF
                     TESGPTQIADFNPYGVYKALTSSKQRNVRSRTDYTKGQPVLNVLTPLATKESWIRVSG
                     VDFGQGATGFRVTAASVAEGNKVEIHKGSVTGTLAGTCDLKNTGNWNSYEDNECEVTS
                     LSGIVDQLFLVFKGSKDSTMGLIEWEFIGEGGGSSTTQTPHGGTASILPGKIEVENYD
                     DGGSRRGYSDTDAENKGDAEMRADEGVDLVLGGTGVALGYTAAGEWLEYTVNVENDAN
                     DVSISASVSSGVETSSFCILVDGEQVGDTVKVAKTGEDWSVYETVNIGKTSLTKGPHE
                     VRLMITGDNVNVDWISFGDIPISISQTKFHYNEPMTYQVFDMKGAMLGTVKLDRLNAT
                     QALRAAGFTKGIYMLKQVQGNKKFVVNATR"
     gene            complement(2308..3153)
                     /locus_tag="PLT31_06210"
     CDS             complement(2308..3153)
                     /locus_tag="PLT31_06210"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW94767.1"
                     /translation="MNDWTTIIKPKTSLFHVDFKELWQYRDLYRMFVKRDIVTWYKQT
                     ILGPLWFFIQPILTTLMFMIVFGGIAQISTDGLPQPLFYLAGICLWQYFSESLTQTSN
                     TFISNTNVFGKVYFPRLVVPLATVTSNLVRLAIQMSLFLIVYLYYFIFTDAAIQPNLY
                     LLLTPVLILLLAGLALGFGILFSSMTTKYRDLTFLLSFCVQLWMYATPVIYPLSTIKN
                     EKLHFLMSLNPITSIIETFKYATLGVGSYSPYALLYSAIFTVLILCTGILVFNRIQKT
                     FMDTV"
     gene            3312..5024
                     /locus_tag="PLT31_06215"
     CDS             3312..5024
                     /locus_tag="PLT31_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94768.1"
                     /translation="MRCLENILSGFMSVKRSLLIALAINLFFLVLCVLFGDLRFGAID
                     DYFMAAVLTGAHGDSYNPLIVFVNALYAYALLPLYHLFPSVGWYYLGLMASVFFSFLT
                     ITFIILEVLGLRLGSVLSALFVALFASDFYLVCQFTQTASILSASGMLCLIWAAFYKR
                     HLGFMVWGAFLLFLGSLIRYQAFMMGLPFLGITMLFFIKDLWPSKWRLGITLTVTLIA
                     IFAAHSFDQQLYQNNESYKDFMAFQAPRVVLGDGSDYNQQAVYEDLEEMGFSGRDFAL
                     LKDWTFYDTETFSVKNLSPIVQTIPRYKYSVKWQRMPQFILSALMKSARSPLFWAWVI
                     LSLALFQSRKKMVFYPWATLGLIFCLMTYLLMMNRLVYRVESGFWLYAACLTIPFLKS
                     FSLLETHKIFKKSYWVFAFLTLIAVVNLVQYVRAGEIVRDPSTGAKRTLTLENDSTDY
                     EQVFKYIEERPDFVFLTSMNAYMRFSHHKMPPWRAEPFGSYKNIIPLGYWTPYLPEVT
                     RELEKRGVKNPMKEAIHPNVIVINEGRLNDFLERHYYDKVKVDTVKAIGEMIFYKYSL
                     EEGL"
     gene            5021..>6204
                     /locus_tag="PLT31_06220"
     CDS             5021..>6204
                     /locus_tag="PLT31_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94769.1"
                     /translation="MKRFLYPLVATALITIAPFLLTFEKGQDIVRGLFALSILALLLL
                     FTFIKEQPLKIKGSKTALLGLVALLVLGVAWIDLQNILFLQNRSTGYFAFIPLITTFM
                     ALLILKPIQALSMRNSSLFLFLFIVAHLVWMQFSPSQPLAEFPIIKSLSNTSKTVQRD
                     SLPSTIKEEYLVIDTATITARYIDSSKSNVVILVESWGVPLDTESRQKAFQIFDDFSV
                     IKGIHSRVYSRTRTAEREDLLLNLSKQGKKNDSIFIPKVFSEQQIQTKFFFGGDSTVQ
                     NREEYIRNMGFEEAFFFPSGASDSFVLGVIDSTLGAHENEKLLIAWTTRDTRFPIPGD
                     VSTIEKVYYDKLLNTLKGIAALAQKHPHVRFIVQGDHEPILSPIAFQERFYKRFVPFI
                     IL"
BASE COUNT         1792 a         1127 c         1302 g         1983 t
ORIGIN      
        1 tgtcaaatta tccataacaa atgccctaaa ggcgttttag taccccaaaa cttgatttat
       61 tttatgttag ggatattgtc tttgtataaa ataaaacaga aggttatctt atgatgaatc
      121 taaagaaaac ggcctttttg gtcactttca cggtgttggt atttttcggc ttttcaactg
      181 cagataaccc aatttccaat tatcattatc ttgccgatcc tacggctttt tcaaccgatt
      241 caacgttcta tatcgttacc gatacggatg atgaatgtcc aagtactggt gaaaatgact
      301 ataaaattcg tgccttgtat gcttttagta gtaaagacat gaaaaactgg actgatcatg
      361 gtatggttct tcgctataat cgtgaagtct cttatatttc aaatatttgg gcctctgggg
      421 ctgtagcaca cgatggcgtt ttttacatta tttacccaaa tggggcgagt ggtgttggta
      481 ttgtgtcttc taaagatatc gctggccctt acattgatcc agttggtaaa ctcttagtcg
      541 gcggtggtgg cgtcacggat tgcggaggca ttagttggtg ctttgaccca gccatttttg
      601 tggatgacga tggaaaatca tatatgactt atggtggtgg cagcagtgga tctcgtcctt
      661 atggcgataa ttttgatctt tttcagttta gtaaactttc acacgatcaa gtcactttag
      721 atgttaattc cagaactcga atttcaggag cgaataagtc ttttgaagca tcttatattc
      781 acaaaagaaa cggaacctac tattttagtt ataatgatca aagccaagcg attagctacg
      841 ctacatctaa aaacatcaaa gggccttata cataccaagg cgtagtgatt aataatcctt
      901 cttcaattaa tggtaaaggg ggcaacaacc atcatggttt tgcacagttc aaagataaat
      961 ggtatgctgt ttatcatgac cgtcgcttag ttatggatcc agaacatcca gcagcaggtg
     1021 gtcaaatcaa tggtttaaca acaggaaacc atcgtagtac aagtatcgat gaagtgactt
     1081 ggaatggcga taaaatgaat gtggtgaagt ttaccgaatc aggcccaaca caaattgcag
     1141 atttcaaccc ttatggtgtt tataaagctc ttacgagttc caaacaacgt aatgtgcgta
     1201 gccgtacgga ttacactaaa gggcagcctg ttttaaacgt acttactccg cttgcaacta
     1261 aggaatcttg gattcgtgtg agtggcgtcg attttggtca aggcgccact ggatttcgag
     1321 ttactgccgc gagtgtagcc gagggcaata aggttgaaat tcacaaaggt tctgtaacgg
     1381 gtactttggc tggtacttgt gatttgaaaa acacaggtaa ttggaattca tacgaagaca
     1441 acgaatgcga agtcacaagt ctctctggaa tcgtggatca acttttcctt gtgtttaagg
     1501 gatctaaaga ttctactatg gggctcattg aatgggaatt tataggggaa ggtggcggct
     1561 cttcaaccac gcaaactcca catggtggca ccgcgtctat acttccagga aaaatcgaag
     1621 ttgaaaatta tgatgatggt ggcagtcgcc gcggctatag cgatactgat gccgaaaaca
     1681 aaggcgatgc agaaatgcgt gcagatgaag gtgtcgactt agttctcggt ggcacgggtg
     1741 tggccctagg ttataccgct gctggagaat ggcttgaata tacagtcaat gtggaaaacg
     1801 atgctaatga tgtttcaatt tcagcaagtg tttcttcggg tgttgaaact tcgagtttct
     1861 gcatcttggt tgatggagaa caggtcggcg atacagttaa ggtcgctaaa acaggcgaag
     1921 attggagcgt ttatgagact gtgaacattg gaaaaacatc tcttactaaa ggtccacacg
     1981 aagttcgcct tatgattaca ggagacaacg tcaacgtcga ttggatttct tttggagata
     2041 ttcctataag catttctcaa acaaaattcc actataacga accgatgact tatcaagtgt
     2101 ttgatatgaa gggagcgatg cttggcactg tgaaattaga tcgattgaat gctacgcagg
     2161 cgcttcgtgc cgctggattt actaaaggca tctatatgct caaacaagtg cagggcaata
     2221 agaagtttgt ggtgaacgct actcgataag agtttaattc gacatatttt tttgaatagg
     2281 ccactagctt aatcgctggt ggttttttta taccgtatcc ataaatgttt tttgaattcg
     2341 attaaaaact agaataccag tacataaaat taaaacggta aaaatagcac tataaagtag
     2401 agcataaggg ctatagctgc ccacacccaa tgtggcgtat ttaaatgttt cgataatact
     2461 tgtgattggg tttagtgaca ttaaaaagtg cagtttttca tttttaatag tgctaagtgg
     2521 gtaaatcact ggcgtggcat acatccaaag ctgaacgcaa aaagaaagta aaaatgttaa
     2581 gtctcggtat ttggttgtca tggaagaaaa taaaatacca aaacctaatg ccagccccgc
     2641 tagtaaaaga attaaaacgg gtgtgagtaa taaatagagg ttgggttgaa tggcggcgtc
     2701 agtaaaaatg aaatagtaaa ggtaaacaat taagaaaagg ctcatttgaa ttgctaaccg
     2761 cactaaattt gaggtcacag tcgctaatgg aacaacaagc cttggaaaat agaccttacc
     2821 aaagacatta gtattagaaa taaaagtgtt cgatgtttgc gtgagggatt ctgaaaaata
     2881 ctgccaaagg caaatgcctg ctaaataaaa aagaggctgt ggaagtccat cggtgctgat
     2941 ttgcgcgatg cctccaaaga caatcataaa cataagagtg gttaatatcg gctgtataaa
     3001 aaaccaaaga ggcccaagaa tcgtctgctt ataccaagtg acaatgtctc gctttacaaa
     3061 catccgatat aaatcacggt attgccacaa ctctttaaag tcaacgtgaa ataagctcgt
     3121 tttaggcttt ataatggttg tccaatcatt catatttcaa agatataaaa taatcttcca
     3181 ttgaagctga aaataggggt ttaagaacaa gtctaagggc cttaaaaaat tgtttgaatt
     3241 gattctttta taagtatatt gtttagcatc tttgtttatg aacggtgaaa agcaattata
     3301 cttttatgat gatgcgttgt ttagaaaata ttttaagtgg ctttatgtct gtgaagcgtt
     3361 ctttgttgat cgctcttgca ataaatcttt tctttttagt gttgtgcgtt ctttttggcg
     3421 accttcgttt tggtgctatt gatgattatt ttatggcggc agtgctcact ggtgctcatg
     3481 gcgattctta taacccgctg attgtttttg taaatgctct ttatgcttat gctcttttgc
     3541 ctttgtacca tttgtttcct tcggtgggtt ggtattatct cggattaatg gcgagtgtct
     3601 ttttttcttt tttaacaatc acatttatta tactagaggt tctcggtctt cgcttagggt
     3661 cagtcctttc cgctttattt gttgctctct ttgcctctga tttttatttg gtctgccagt
     3721 ttacgcaaac agcttctatt ttatctgctt ctggaatgct ttgccttatt tgggctgctt
     3781 tttataaacg tcacttaggc tttatggttt ggggagcttt ccttttgttt ttaggctctc
     3841 tgattcgtta ccaagcgttt atgatggggc tgccgttttt aggcatcacc atgctctttt
     3901 ttataaaaga cttatggcct tcgaaatggc gcttaggtat taccttaacc gtcactttaa
     3961 tcgctatttt tgcagcccat tcttttgatc aacagctata tcaaaataac gaatcttata
     4021 aagattttat ggccttccaa gcgcctcgtg ttgtgttagg ggatggcagt gattataatc
     4081 agcaggcagt atatgaagat cttgaagaaa tgggcttttc aggtcgagat tttgccttgc
     4141 taaaagattg gacgttttac gatacagaaa cttttagtgt taaaaactta agccctattg
     4201 ttcaaacgat cccgcgatat aaatacagtg tcaaatggca aagaatgccc caatttattt
     4261 taagtgcctt gatgaaatca gcgagatccc cattgttttg ggcttgggtt attttgagtt
     4321 tggcgttgtt tcaaagtcga aagaaaatgg ttttttatcc ttgggctacg cttggattaa
     4381 ttttttgctt aatgacctat ttgttaatga tgaatcgctt agtttaccgt gtagagagcg
     4441 gcttctggct ttatgctgct tgtttaacaa ttccattctt aaaatctttt tcacttttag
     4501 aaacgcataa gatatttaaa aaatcctatt gggtatttgc attcttaaca ctgatcgccg
     4561 tagttaatct agtccaatat gtaagagcag gtgaaattgt acgagaccct agcacagggg
     4621 caaaacgcac attgacttta gaaaatgatt cgacggatta tgaacaagtc tttaagtata
     4681 ttgaagagcg ccctgacttt gttttcttaa caagcatgaa tgcttacatg cgtttttctc
     4741 atcataaaat gcccccttgg cgtgcagagc cttttggcag ttataaaaac ataattcctc
     4801 ttgggtattg gactccgtat ttacccgagg taactcgaga gcttgaaaag cgtggtgtga
     4861 aaaatccaat gaaagaggct attcacccaa acgtgattgt catcaatgaa ggacgcctta
     4921 atgacttttt agaacgccat tactatgaca aagtcaaagt cgatacggta aaggctattg
     4981 gagagatgat cttttataag tacagtttag aggagggttt atgaaacgtt ttctctatcc
     5041 tttagtagcc acagctttga ttacaattgc accttttttg ctgacttttg aaaaagggca
     5101 agacattgtg cgaggtcttt ttgctctcag cattttagct cttttattat tgtttacttt
     5161 catcaaagaa cagccactaa aaataaaggg gtcgaaaaca gcgcttttag ggcttgtagc
     5221 acttttagtt ttaggtgtcg cttggattga tttgcaaaac attctctttt tacaaaatcg
     5281 ctccacaggg tattttgctt ttattccttt aatcacaacg ttcatggctc tcttgatttt
     5341 aaagcccatt caagccctgt cgatgcgtaa cagtagcctt tttctatttc tctttattgt
     5401 ggcgcattta gtctggatgc agttttcgcc ttctcaacca ttagccgaat tcccaatcat
     5461 caaatcatta agtaatacgt caaaaacagt ccaacgagat tcactacctt ctactattaa
     5521 agaagaatat cttgtgatcg ataccgcgac cattacagca cgctatatag actcttctaa
     5581 aagcaatgtg gtgatacttg tggagtcttg gggcgttcca ctcgatactg aatcacggca
     5641 aaaagcattt caaatatttg acgatttttc tgtaatcaaa ggaatccatt cgagagtata
     5701 ttctcgcacg cgtaccgcag agcgtgaaga cttgcttttg aacttaagta agcaagggaa
     5761 aaagaatgat tcaatattta ttcccaaagt attttcagaa caacagattc aaaccaagtt
     5821 cttctttggt ggtgattcta ccgtacaaaa tagagaagaa tatataagaa atatggggtt
     5881 tgaagaggcc ttctttttcc cgtctggagc aagtgattct tttgtgcttg gcgtgataga
     5941 ttcgactttg ggcgcgcacg aaaatgaaaa gctcctgatc gcttggacta ctcgagacac
     6001 gcgtttccca attccaggag atgtgtctac aatagaaaaa gtgtattatg ataaactttt
     6061 gaatacgctc aaaggtatcg ctgcattagc ccaaaaacat ccacacgttc gttttatcgt
     6121 tcagggcgac catgaaccca tcctctcgcc catagccttc caagaacgct tctacaaacg
     6181 ctttgtacca ttcataatcc tgaa
//
LOCUS       DAOOBB010000116         6127 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_14996_length_6127_cov_4.101614, whole genome shotgun sequence.
ACCESSION   DAOOBB010000116 DAOOBB010000000
VERSION     DAOOBB010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 6127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6127
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14996_length_6127_cov_4.101614"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(229..567)
                     /locus_tag="PLT31_06225"
     CDS             complement(229..567)
                     /locus_tag="PLT31_06225"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPW94770.1"
                     /translation="MKFAIPLAEGKLTTHFGHCKSFALLTVNTGETTITQKTEVEAPP
                     HKPGLLPVWLHEQGVTHVIAGGLGQRARDLMEEKGIQVFTGAPCLEPEVLVNQFLTNK
                     LVLGANGCDH"
     gene            complement(743..1651)
                     /locus_tag="PLT31_06230"
     CDS             complement(743..1651)
                     /locus_tag="PLT31_06230"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPW94771.1"
                     /translation="MKINKPKELVVISGKGGTGKTSITAALAQCFSTKVLADCDVDAA
                     DLHLLMKVKSYKEYPFIAGHIAQINEEKCQNCGLCATLCRFDAITQTDNLYKISKQSC
                     EGCKVCVDACPNKAIEWIESQTGVWKETYTEQGLMFHARLFPGAENSGKLVSHVREKA
                     KQYAVSNQIPLILVDGPPGIGCPVIASVTGASAVLIVTEPTPSGHHDMKRILSLTKHF
                     KIPAYVIINKADLSDEFCTRIQETSEQFNSIVVGKISYNSLFTEAQLLGKSILEVFPE
                     SNESKTIKSIANAMSHKMELQISRND"
     gene            complement(1648..2484)
                     /locus_tag="PLT31_06235"
     CDS             complement(1648..2484)
                     /locus_tag="PLT31_06235"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW94772.1"
                     /translation="MKIAIASGKGGTGKTSLAVALLHANAERARLLDCDVEEPNCHLF
                     LSHTVIGHKAVELLIPQVNPNLCKLCGKCVEACQFNAIAIAGNQVMIFNELCHSCGGC
                     ARICPHGAIQEFPVKIGELSQSRCKNEAELLTGTLEIGHASAPAVIKAAKKALPLQDV
                     PFTLIDSPPGTACTFVAAVEDSDFVFLVTEATPFGLNDLKLAVEALLNMELPFAIIVN
                     RIQSETNLITDYANQQGFPIWLQIPEDRHIAETYARGGNLLDVKPELKKVLRELLDRC
                     QP"
     gene            complement(2484..2843)
                     /locus_tag="PLT31_06240"
     CDS             complement(2484..2843)
                     /locus_tag="PLT31_06240"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPW94773.1"
                     /translation="MKIVFSTEGESLESLLDTRFGRCSRFLLWDFETKTFTLIENSQN
                     LQALQGAGIQSAQNVINTGAEALVTGHCGPKAFKVLQAAGIPIYVAESLPIRELIDSF
                     EKGTLLQLKEADVEGHW"
     gene            complement(2840..3025)
                     /locus_tag="PLT31_06245"
     CDS             complement(2840..3025)
                     /locus_tag="PLT31_06245"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HPW94774.1"
                     /translation="MSNRDGTGPLGQGSQTGKALGSCNNKQLFPSNQSFGGAMSRGNR
                     KGKGNRLNNSNFGRGQS"
     gene            3119..3433
                     /locus_tag="PLT31_06250"
     CDS             3119..3433
                     /locus_tag="PLT31_06250"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HPW94775.1"
                     /translation="MPRRLKHRCCRSDASGLYYKPRGVPMADLESELLQLDEFEAMRL
                     CDLDGLEQGAAGDKMGVSRGTIQRLLETGRKKLIQAIVEKKSIQIGEFPSCCKNGKRC
                     DE"
     gene            3430..4257
                     /locus_tag="PLT31_06255"
     CDS             3430..4257
                     /locus_tag="PLT31_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPW94776.1"
                     /translation="MNSVMLTVVVDNNSELLNAPSEHGLSLHLQSDNHNMLFDAGKSD
                     LVLANLEALGLAAKSPEWIALSHGHYDHTTGVPALLSRYPNAKLYFHPKLLEQKWILD
                     PGDQWRYGGLPSAFFELDALYLQPHRSDMELIPGVFTSGSVVGDDAQSAVRSRFFRIS
                     NGCKCIDAFPDEQVLFVKTNKGVSIISGCMHTGIEATIKKARERFPQEPFYALVGGLH
                     LEGKTQKEFETILNIIHEAEFRIVMPLHCTGKGFVDYLANHDSALLLSGSVGKTIEL"
     gene            complement(4261..4818)
                     /locus_tag="PLT31_06260"
     CDS             complement(4261..4818)
                     /locus_tag="PLT31_06260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HPW94777.1"
                     /translation="MESSAAVAGIKKNKKSWCYHNKIENGEVAGRLYTWAAAIDSVSL
                     AEDSENPITCGTSVECTLPETVQGICPEGWHLPSSEEWTILETFVGGNTEAGTYLKSK
                     AGWKKYVDVSNLDSFGFSAFPAGQIHLTLSLNFDNGLYTTSWWTATENSSIYANPRYM
                     TFSKNYIGSVNLSKIVGYSVRCIKN"
     gene            4790..5317
                     /locus_tag="PLT31_06265"
     CDS             4790..5317
                     /locus_tag="PLT31_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94778.1"
                     /translation="MPATAAEDSIKMSNSSIEEPYPLLEVDDPIFFFPWNIFIGGGLG
                     MFSVGLDLTVHPYSELYATKRPDSIEGMWNPPHILLWANRARMIYDFYNHQYGFFLQP
                     NIQYFYDVIMVTVTLGPEIGWQTETGFDYGASIRIGGFIGVLLGVYEFGYLVNAKRFY
                     FNFLFNMPIGIASSV"
     gene            complement(5325..>6127)
                     /locus_tag="PLT31_06270"
     CDS             complement(5325..>6127)
                     /locus_tag="PLT31_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94779.1"
                     /translation="PQNSNRIEKAISKLKDKEQRAFTRIQILEYYTKYYPFDLEHKNI
                     FNANIDSISNHKLINPLSPFFNFPYVNNKKEKLFYLYFGANYSWLMNGAEKTFYNRHQ
                     LGFNFFGAGFCKDIFCLDFDFMALFSKKDGESRIENDSIFYRESPDMRISALLQYRFF
                     RTDQFDFRIFASYIWNAYSFKTNESDSTIVYNNIGDFFDWGIGGIATYYFKDLYISPQ
                     LVSLALRTKVEYVFLDTPKFNNLHGNSLNVSLSFVFNFILQPSLKYAY"
BASE COUNT         1750 a         1142 c         1188 g         2047 t
ORIGIN      
        1 tagattttcc gtcatatagt gcgagccgtt caccataaat aaaaaaagac tcacctttag
       61 gtgagttttt ttttatttat ttagtttgta acatactacg cccggatgag ttcgcgtgca
      121 atgactcctc tgctacgagc ttgccgagcg ttgttgtttc tcctcgtcat agctgatgct
      181 atgtcaccgt cgtcacgcct tgtttggtac gtgctccacg ggatgctttt aatggtcaca
      241 tccattcgca ccaagtacta gcttgtttgt caaaaattga tttacaagta cttcaggttc
      301 taagcatgga gcacctgtaa atacttgaat gcctttttct tccattaaat ctcgagctct
      361 ttgtccgaga ccacctgcaa ttacatgagt tacaccttgt tcgtgtagcc atacagggag
      421 taaacctggt ttgtggggtg gtgcttctac ttctgttttt tgagtgattg tggtttcacc
      481 ggtatttacg gtaagtagag caaagctttt acaatgacca aaatgggttg tgagcttgcc
      541 ttctgctaaa ggaattgcaa atttcatgtg ttctccttgt gaatttgttt gaaaatttcc
      601 gtaattacgc gcattttgtg cgtgatccaa tgctatttct gaatattggg tatgtgcttg
      661 tactgtcatt ctttttttcc ttgcatgaaa accagttctc cttttctgat ggccgttagc
      721 ttagccatag tgttagattt acctagtcgt ttctggatat ttgcaactcc attttatgtg
      781 acattgcatt ggctatggat ttaatcgttt tagattcgtt actttcaggg aatacctcaa
      841 gaattgattt tccaagaagt tgggcttctg taaacaatga attataggaa atttttccaa
      901 caacaatgga gttgaattgc tctgaggttt cttgaatgcg agtacagaat tcgtcactaa
      961 gatccgcttt attgatgatt acataggcgg gaattttaaa gtgtttagtt agtgataaaa
     1021 tgcgtttcat atcatgatga ccagatggcg ttggttctgt aactatcagc actgcacttg
     1081 ctcctgtaac agaagcaatc actgggcagc caataccagg tgggccatcg actaaaatca
     1141 aaggaatttg atttgacacg gcatattgct tcgctttttc tcgtacatga ctcactagct
     1201 tgcctgagtt ttctgcacca gggaaaagcc gtgcatggaa catgagacct tgttccgtat
     1261 aggtttcttt ccatacgcca gtttgagatt caatccattc aatcgccttg ttagggcagg
     1321 catctacaca gactttgcat ccttcacaag actgtttact gattttgtat aaattatctg
     1381 tttgtgtaat cgcatcgaaa cgacaaagtg tagcacataa gccacagttt tgacattttt
     1441 cttcattgat ttgagcaata tgacctgcta tgaaaggata ttccttatag gactttactt
     1501 tcatcagcaa atgcaaatcg gcagcatcca catcacaatc agcgagtacc tttgttgaaa
     1561 aacattgggc taatgcagcg gtaatagatg ttttccctgt tccacccttt ccgctgataa
     1621 ccacaagttc cttgggctta tttattttca tggttgacac ctgtctaaaa gttcacgcaa
     1681 cacttttttt aattcgggtt ttacatccaa taaattgcct ccgcgagcat aggtctctgc
     1741 tatatgacga tcttctggaa tttgcagcca aattggaaat ccttgttggt ttgcataatc
     1801 agtgattaaa ttggtttctg attgtattcg attgactatg atggcaaatg gtaattccat
     1861 attaagtaac gcctccacag ctagttttaa atcatttaat ccaaagggag ttgcttcagt
     1921 aacaagaaat acgaaatcag aatcttcaac tgctgcaacg aaagtacaag ctgtacctgg
     1981 gggagaatca atcaaagtaa aaggaacgtc ttgtaagggg agtgcttttt tggcggcctt
     2041 gatgactgca ggtgcagaag catggccaat ttccaaagtg cccgttaata attcagcttc
     2101 atttttacag cgtgattggc ttaattctcc gatcttcact ggaaattctt ggatggctcc
     2161 atgtggacag attcttgcgc atcctccaca actatggcaa agctcattga agatcattac
     2221 ctgatttcca gctatggcta ttgcattaaa ttggcatgct tctacgcatt ttccacagag
     2281 cttacataag ttaggattca cttgaggaat caatagttct acggccttat gtcctataac
     2341 agtatgagat aagaataggt gacaattggg ttcttccaca tcacaatcta aaagccttgc
     2401 tcgctctgca tttgcatgta aaagtgcaac agcaagactt gttttacccg taccaccttt
     2461 gcctgatgct attgcaattt tcattaccaa tgcccttcta cgtctgcttc ttttaattga
     2521 agtaaggttc ctttttcaaa agaatcaatc aactctcgta tgggtaggct ttctgcaacg
     2581 taaattggta ttccagcggc ttgaagtacc ttgaatgctt taggtccaca atgtcctgtt
     2641 actaaagctt ctgcaccagt gttaattaca ttttgtgcag attgaattcc agcaccttgt
     2701 agtgcttgta aattttgaga attctctatt agtgtaaaag ttttggtttc gaaatcccat
     2761 aataagaatc tagaacatcg cccaaaacga gtgtcaagga gagactctaa ggattctcct
     2821 tcggttgaaa aaacgatttt catgattgcc cccttccaaa attagagtta tttagtctgt
     2881 tgcctttccc tttacgattt cctcggctca ttgcccctcc gaaagattga tttgatggaa
     2941 ataattgctt gttattgcat gaacctaagg cttttcctgt ctgtgaacct tgtcctagtg
     3001 gtcctgttcc gtctctattt gacataattc ctccattatt tttgaattgt gcatatgcaa
     3061 atatacattt cttttttata ttttgtgcat aagcacgcat ttattttgga aaaattttat
     3121 gccaagacgt ttaaaacatc gctgctgtag gtcagatgct tctggtttat attataaacc
     3181 aaggggagtt cctatggctg atcttgaaag tgaattattg cagctcgatg aatttgaagc
     3241 catgcgtttg tgtgatcttg atggccttga acaaggggcg gctggagata aaatgggcgt
     3301 gtcccgtggt actatacaac gtcttcttga aacaggaaga aagaaattga tacaggccat
     3361 agtagaaaag aaatcaattc agatagggga attcccatct tgttgcaaaa atggaaaaag
     3421 gtgtgatgaa tgaattctgt aatgttaact gtagtggtag ataataattc agagctctta
     3481 aatgcaccca gtgaacatgg gctctctttg catcttcaaa gtgataatca caacatgtta
     3541 tttgatgcag gtaaatcaga tctcgtactt gctaatcttg aggcgcttgg tcttgctgca
     3601 aaatcacctg aatggattgc tcttagtcac ggtcattatg atcataccac tggggtacca
     3661 gctcttcttt ctcgttatcc aaatgcgaaa ctctattttc atcctaaact acttgaacaa
     3721 aaatggatat tagatcctgg agatcaatgg cgatatggtg gattgccttc tgcttttttt
     3781 gagctagacg cattatattt acagcctcat cgttctgata tggaattgat tcctggagta
     3841 tttacaagcg gttctgttgt aggagatgat gcacagagtg ctgttcgttc tcgttttttc
     3901 agaatttcta acggttgtaa atgtattgat gctttccccg atgaacaagt cttatttgta
     3961 aaaacaaata agggtgtttc gattatcagt ggttgcatgc atacaggcat tgaagcaacc
     4021 attaaaaaag ctagagaacg ttttccacaa gaaccattct atgctttagt tggtggcctt
     4081 catttggaag gaaaaacaca aaaagaattt gaaactattt tgaatattat tcacgaggca
     4141 gaatttcgaa tcgtaatgcc tttgcattgt actggaaaag gtttcgttga ttatcttgca
     4201 aatcatgatt cagcactatt attgtctggt tctgtaggga aaactattga actctaatat
     4261 ctaatttttt atacaacgga cggagtagcc gacgatttta gataaattga cggaacctat
     4321 atagttttta ctgaacgtca tgtatcgagg atttgcatat atggagctgt tttctgttgc
     4381 tgtccaccaa gaggtggtat agagtccatt atcaaaattt aatgataagg taaggtgaat
     4441 ttgacctgct gggaaggctg aaaatccaaa agaatctaga ttagagacat caacatattt
     4501 tttccaacca gcttttgatt ttaaatacgt tccagcttcg gtgtttccac ctacaaaagt
     4561 ttcaagaatc gtccattctt cagaagaggg gagatgccag ccttctgggc aaatgccttg
     4621 aacagtttct ggcaaagtac attcaacgct cgtaccgcat gtgattggat tttcggagtc
     4681 ttctgcaagt gaaacggaat caatcgctgc agcccaagtg tacaagcgac ctgccacttc
     4741 gccattttca attttattat gataacacca agatttctta ttttttttta tgcctgcaac
     4801 ggctgcggaa gattccataa aaatgtctaa ctcatcaata gaagaaccat atcctctttt
     4861 ggaagtggat gacccaattt tctttttccc gtggaatatt tttattggag gcggcttagg
     4921 aatgttctcc gttggattgg atttgacagt gcatccatac tcagaactat acgctaccaa
     4981 acgtcctgac tccatcgaag gaatgtggaa tccgccacat atattacttt gggcaaatcg
     5041 agctcgcatg atttatgatt tctataatca tcagtatggc ttttttttac agcccaacat
     5101 ccaatacttt tatgatgtga ttatggtgac tgttacattg ggaccagaaa ttggttggca
     5161 gacggaaaca ggctttgatt atggggcatc cattcgaata ggtggtttta ttggggttct
     5221 tttaggcgtt tatgagtttg gatatttagt caatgcgaaa cgattttatt ttaactttct
     5281 ctttaatatg ccgattggca ttgcatcctc tgtttagata gaccttaata agcgtatttt
     5341 aaactaggct gaagtataaa attaaataca aaagacaaag acacgttcaa tgaattgcca
     5401 tggagattat tgaatttagg agtatctaaa aagacatact ctacctttgt tcttaaagcc
     5461 aaagaaacta actgtggtga aatatataag tccttaaagt aatatgtagc aatacctcca
     5521 atgccccaat caaaaaaatc acctatgttg ttataaacta tagtagaatc actttcgttg
     5581 gttttgaaac tataagcgtt ccaaatgtag cttgcaaaaa ttcgaaaatc gaattgatct
     5641 gttctaaaaa aacgatattg caatagggct gaaattcgca tatctggaga ttctcgataa
     5701 aaaatagaat cgttttctat acgagactct ccatcttttt tactgaataa agccataaaa
     5761 tcaaaatcta aacaaaagat atctttgcaa aatcccgcac caaaaaaatt gaacccaagt
     5821 tgatgacgat tataaaatgt tttttcagca ccattcatta gccaagaata attagctcca
     5881 aaatacaagt aaaaaagctt ttctttttta ttattcacat aaggaaaatt aaagaaaggg
     5941 ctaagtggat taattaattt atgattactt atgctgtcta tgtttgcatt aaaaatattt
     6001 ttgtgttcta aatcaaatgg atagtatttt gtatagtatt ctaaaatttg aatgcgagta
     6061 aaagcacgtt gctctttatc ttttaatttg ctaatggctt tttcaatacg attcgagttt
     6121 tgaggat
//
LOCUS       DAOOBB010000117         6119 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_15018_length_6119_cov_3.529354, whole genome shotgun sequence.
ACCESSION   DAOOBB010000117 DAOOBB010000000
VERSION     DAOOBB010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 6119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6119
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15018_length_6119_cov_3.529354"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..742)
                     /locus_tag="PLT31_06275"
     CDS             complement(11..742)
                     /locus_tag="PLT31_06275"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF011456.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HPW94780.1"
                     /translation="MSRNIAIMGATGAVGQELLSILEQRQFPIKSLKLLASKRSAGKT
                     IKFMGNDLTVEELTHDSFKGVDLVLSSAGGSISRDYAPSAVKAGAVVVDNTSHYRMDP
                     NVPLVVPEVNPQDIKKHKGIIANPNCTTIIMSLPLFPLYNAFGVERVSVCTYQSASGA
                     GAIAMDELREESAALAHNEPFERTVIPYQYAFNLFPHNSPFNDGSEEKAKIKAPKGYC
                     EEEWKMVAETRKIFGDETIFHSSSQ"
     gene            complement(768..1424)
                     /locus_tag="PLT31_06280"
     CDS             complement(768..1424)
                     /locus_tag="PLT31_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94781.1"
                     /translation="MNGAEIISIAILWCICLLALIQALNARGAIRISLTWIITMVIVI
                     VTVAVTYIQLSDKETNPISSVSFSNTEEINTPSATNVKQNEVRVNTYISSSKKLVAEV
                     TEAIRSIEDFDANKVPSTEAEREVWERQALSLSQQTAKLNQRTLGLFHPRNVSELHGI
                     LLQCTENLRLAGYALHTLSTLEDPELKEVQKEQYLNQIAQAKQKLNLFQIQLKELDQP
                     "
     gene            complement(1436..1903)
                     /gene="ssb"
                     /locus_tag="PLT31_06285"
     CDS             complement(1436..1903)
                     /gene="ssb"
                     /locus_tag="PLT31_06285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPW94782.1"
                     /translation="MAYLNKVMLIGNIGKDPEIRMTTTGRKKVSFSLATSRRYRDNNG
                     ETKEITDWHNVVGWGKTADIIESLGVRKGMSLFVEGTLTYRSWDDQATGQKRYATDVT
                     METFQLLTPRNSNGAPQNGNYNRSSSTPSYDNSSMGYPPASADSADMDDDLPF"
     gene            1957..3051
                     /locus_tag="PLT31_06290"
     CDS             1957..3051
                     /locus_tag="PLT31_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HPW94783.1"
                     /translation="MTVKLAHHRFSEVFPKPLRGKRLGAVLHPASVDASLHHTLDLLK
                     EHNGSLFHLSALFGPQHGIKGHTQDNMIEWEGYLDKELQIPVYSLYGTHREPTEEMLS
                     HVDVLFLDLQDVGARYYTFIWTLYLCMKACESRSIPVVVLDRPNPIKTGVVEGPILDL
                     EYKSFVGLHSIPVRHGKTIGQLARQFQSECFPKTELYVLEMEGYKEDLWFDQTGLPWI
                     IPSPNMPTLDTAIVYPGMCLFEATNVSEGRGTTKPFEFFGAPFIDAEELCRYMNGLHL
                     PGVYFRENYFQPTFHKGANQINGGAQIHVLNRDVFDSFTMAIEILKFLFHRYPNDFAW
                     KQPPYEYEYKKLPIDILLGSGTFRKEFIEK"
     gene            complement(3201..4397)
                     /locus_tag="PLT31_06295"
     CDS             complement(3201..4397)
                     /locus_tag="PLT31_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-2-enoyl-CoA reductase family protein"
                     /protein_id="HPW94784.1"
                     /translation="MIIKPLVRSNMCLNAHPVGCAKDVERQINYILSKKKQKNLSDQK
                     PKIVVVLGCSTGYGLASRIAAAFEYDATTIGVSFERPGTETKSGTPGWYNNMAFDKAA
                     KEKGLKSITFNMDAFSDEARVQVIKQLQEMGVQADLIIYSLASPVRTDPETGVLYRSV
                     LKPIGENFSGNTIDIISSKFSAIHVEPANQEEIDNTVKVMGGEDWERWITQMQKANVL
                     ASGCKTVAYSYIGPSLSHAIYRDGTIGEAKKHLEKTAKHLNSFLKENVAGEAYVSINK
                     GLVTRSSAVIPIMPFYISILYKVMKRNGSHEGCIEQCERLFSDRLYTGGSVPVDENGL
                     IRLDEFEMDPEVQKEVAETILKISEENLNEMSDFKGFLHDFYIIHGFDVEGVDYSAEV
                     DRLDQL"
     gene            4696..5586
                     /locus_tag="PLT31_06300"
     CDS             4696..5586
                     /locus_tag="PLT31_06300"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HPW94785.1"
                     /translation="MINVLILAAGLGSRLRPLTTDLPKPLVPIVDQSILAHQLRLAKS
                     LESISLHINAFYLAEKLKKEANALGIEHVWVESPEILGTGGPLRRLYQEGVRGDLLVL
                     NGDCYTEIHLAQFLSNSEASGAFISLLGIDEPKVNTLNIDSNSFLSGVANSYGEQSTE
                     KATFSGISWYKEDALKEILDSERNVVHFWKRMNQLNNKIYVDLSQKNALWIDMGTPEG
                     LMKANLARLNELGIDSWVDPLHPLAKQKNNFHQSVVHKHAKVALGAVLNRVILFDGAE
                     VLENEVVSNQIRGDGFRWNL"
     gene            5586..>6119
                     /locus_tag="PLT31_06305"
     CDS             5586..>6119
                     /locus_tag="PLT31_06305"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPW94786.1"
                     /translation="MLEFVIVLVEPEHPHNVGFVARAMCANGLTDLRIVYQKHNSVLA
                     DSYRTAHASQSILDHAKITKNLKEAIQDVQYTIAFSRRTFESVVPVISLPSIQTILPK
                     EGKVALVFGRESIGLLLEEINQCAQICEIPVPGNHSLNLGQAVSVALYELLKENKSGL
                     LNDYSSPVSKENSLANKA"
BASE COUNT         1890 a         1102 c         1127 g         2000 t
ORIGIN      
        1 cccctaaggg ttattgtgaa gaagaatgga aaatggtttc atcaccaaaa atcttacgag
       61 tttcagcaac cattttccat tcttcttcac aataaccctt aggggcttta atttttgctt
      121 tttcttcact tccatcatta aatggtgaat tgtgagggaa caaattgaaa gcgtattggt
      181 aaggaatcac tgttcgctca aaaggttcat tatgagctag tgctgcactt tcttctctaa
      241 gttcatccat cgcaattgca cctgcacctg aagcagattg ataagtacaa acactaacgc
      301 gttctactcc aaaagcattg taaagtggaa acaatggaag agacatgata attgtagtac
      361 aattaggatt cgcaataatt cctttatgct ttttgatgtc ctgagggttc acttcaggaa
      421 caactaaagg cacatttgga tccatacgat aatgacttgt attatctaca actacggctc
      481 ccgccttcac agcacttgga gcataatctc tagaaataga accgccagct gaagaaagaa
      541 caagatctac acctttaaag gaatcatgtg tcaattcttc tactgtcaaa tcatttccca
      601 taaattttat tgttttgcct gcactacgtt ttgatgcgag gagctttaga gatttaatag
      661 gaaattgacg ttgttcaaga atagaaagaa gttcttgacc gactgcgcca gtggcgccca
      721 tgatggctat gtttcgactc atatcattta cctttgattt ttgagtgtta tggttgatcc
      781 agttctttta attgaatttg aaataaattc aatttttgtt tagcctgagc aatttggttg
      841 aggtattgct ctttttggac ctcttttagt tcagggtctt caagcgtaga caaggtatga
      901 agcgcataac ccgctaatct taaattttca gtgcattgca atagaatccc atgcaattca
      961 cttacgtttc ttgggtgaaa taacccaaga gtacgttgat taagtttcgc agtttgttga
     1021 cttaaactaa gtgcttgacg ctcccagact tctctttcgg cttctgtact tggaacctta
     1081 ttcgcatcga aatcttctat tgagcgaatg gcttctgtta cttcagcaac gagtttcttt
     1141 gaagatgaaa tgtaggtgtt tactcgaact tcattttgtt tcacattggt tgcacttgga
     1201 gtattaattt cttctgtgtt agaaaaagaa acgcttgata tgggattcgt ttccttatca
     1261 ctcaactgaa tgtaagtcac agcaaccgta acgatcacga tgaccattgt aataatccaa
     1321 gtcaaagaaa tgcgaatagc tcctctggcg ttcaatgctt gaattaaagc aagcaagcat
     1381 atacaccaaa gaattgcaat tgaaatgatt tcagcaccgt tcatttataa gcctattaga
     1441 aaggtaaatc atcgtccata tctgctgagt ctgctgatgc aggagggtaa cccatgctgc
     1501 tattgtcata actaggagta gatgatgaac gattataatt tccgttttgt ggagctccat
     1561 tggaattccg tggagttaat agctgaaagg tttccatcgt tacgtcagtg gcgtaacgtt
     1621 tttgtccagt ggcttgatca tcccagctac gataagtaag agtaccttca acgaaaagac
     1681 tcatcccttt tcgaacgcca agagattcaa taatatcagc agtttttccc caacctacaa
     1741 cgttatgcca gtcggtaatt tctttggttt ctccattatt atcacgataa cgacgagagg
     1801 tagcaagaga aaatgaaacc tttttgcgac cagtagtcgt catacgaatt tcagggtctt
     1861 taccgatatt tccaattaac atcactttgt ttaagtaagc cataataatt tatgtcctat
     1921 attatattta taatttgaaa aaaaagatag aaaaaaatga cagtaaaact agctcatcat
     1981 cgattctcag aagtgttccc gaaacctctt agaggaaaaa ggcttggcgc agttcttcat
     2041 cctgcatccg tggatgcttc tttacaccac actttagatc ttttaaaaga gcataatggc
     2101 tctttatttc atctttcggc tctttttggc cctcaacatg gcattaaagg gcacactcaa
     2161 gataacatga tcgagtggga agggtattta gataaagaac ttcagatccc agtgtatagt
     2221 ttgtatggaa ctcacagaga accaacagaa gaaatgctct ctcatgtaga tgttttattt
     2281 cttgatttac aagacgttgg agctaggtat tatacgttta tttggacact ttatctttgc
     2341 atgaaggctt gtgaatctcg ctctattcca gtcgtagttt tagatcgccc taatcctatt
     2401 aaaacaggcg ttgtagaagg ccccatttta gatttagaat acaaaagttt tgtaggcttg
     2461 cattctattc ctgtacgtca tggaaaaacg attggacaac tggctcgtca atttcaaagc
     2521 gaatgttttc ccaaaacaga attatatgtt ctagaaatgg aaggctataa agaggattta
     2581 tggtttgatc aaacgggctt accttggatt ataccttctc cgaatatgcc tactttagat
     2641 actgcaatcg tttatcctgg gatgtgtctt tttgaagcca ccaatgtgag tgaaggcaga
     2701 ggaacaacaa aaccttttga attttttggc gctcctttta ttgatgcaga agaactttgc
     2761 cgttacatga atgggcttca tttacctggc gtttatttta gagaaaatta tttccaaccg
     2821 acgtttcata aaggggcaaa tcaaattaat gggggagctc aaattcatgt cttaaatcga
     2881 gatgtatttg attcctttac aatggcaata gaaatattaa agtttttatt ccatcgttat
     2941 cctaatgatt ttgcatggaa acagcctcct tatgaatacg aatacaaaaa gcttcctatt
     3001 gatattcttt taggtagcgg tacgtttaga aaagaattta ttgaaaaata aaagttcttt
     3061 taataaaata agccccgaaa gagaaaaact actttcgggg ctttttatgt aaaaaagaat
     3121 agataaaatt caaaagtttt atcaaaataa aagcccttat tataagggct tttattttat
     3181 gcttgaatat tttttaagga ttaaagctgg tctaaacgat ctacttcggc ggagtagtct
     3241 acgccttcga cgtcaaagcc atgaatgata taaaagtcat gtagaaagcc cttgaaatca
     3301 ctcatctcgt taagattttc ttcgctgatt tttagaatcg tttcagcgac ttctttttga
     3361 acttctggat ccatttcaaa ctcatctaga cggattaatc cattttcatc tacaggtaca
     3421 gaaccccctg tatataaacg atcagagaaa agacgttcac attgttcgat acacccttca
     3481 tgagatccat ttcttttcat tactttgtat agaatagaaa tataaaaagg catgattgga
     3541 attactgcac tagaacgtgt cacaaggcct ttattaatag aaacataagc ttcgccagcc
     3601 acattttctt tcagaaaaga attcaaatgt tttgctgttt tttctaaatg ttttttggct
     3661 tctccaatgg taccatctcg ataaatcgca tgagataaag atggaccaat ataagaatac
     3721 gcgactgttt tacagccaga agctaagaca tttgcctttt gcatttgagt tatccagcgt
     3781 tcccagtctt cgccgcccat gacttttaca gtgttatcaa tttcttcttg gttcgctggt
     3841 tctacatgaa tggcgctgaa tttagaagag ataatatcaa tggtatttcc agaaaaattt
     3901 tccccaatag gttttaatac agatcgataa agaacacctg tctctggatc tgtacggaca
     3961 ggactagcta aactgtaaat aattaaatcc gcttgaactc ccatctcttg aagctgcttg
     4021 atcacttgta ctcgagcttc atcagaaaag gcgtccatat taaaagtaat actctttaaa
     4081 cctttttctt tggcggcctt atcaaaagcc atattgttgt accaaccagg ggtaccgctt
     4141 tttgtttctg ttccaggacg ttcaaaagaa acgccaatag ttgttgcatc atattcaaat
     4201 gcagcagcaa tgcggcttgc taaaccgtaa ccagtggagc aacctaatac aacaacaatt
     4261 tttggttttt gatcagataa atttttttgt ttctttttag aaaggatata attgatctgg
     4321 cgttcgacat cttttgcaca accgacggga tgcgcattca aacacatatt gcttcgaact
     4381 aatggcttaa taatcattat aaaatcctac gttcaatttt gttttgcaaa gttaagaaga
     4441 gtttcatttt ttatcaaacg aaaaaggata aaagaattct gaaacactaa aataaaaatg
     4501 aatatataat caacaagttt ctatttctat ttattgatta tttaatcatt agaatatggc
     4561 ttaaattgat aaaaaaacgg ataaataaga attatcaaat aagaggaatg tcggattgat
     4621 ttgaaaccat agttgatggc tttttaatga gaaaggcata ttaatagggt ttagtttttt
     4681 tataagtttg aattcatgat taacgtatta attctagctg ctggtttagg atcaagactt
     4741 cgaccattaa caacggatct ccccaaacca ttagttccca ttgtagatca gtccattttg
     4801 gctcatcaat tgagattagc gaagtcgcta gagtctattt ctcttcatat aaacgcattt
     4861 tatcttgcgg aaaaactaaa aaaagaggcc aatgctcttg gaattgagca tgtttgggtt
     4921 gaatctcctg aaattttagg cacaggaggc cctttacgcc gactttatca agaaggtgta
     4981 agaggagatc ttttagtatt gaatggggat tgttatacag aaatccatct tgctcagttt
     5041 ctttccaatt cagaagcctc aggagcattc atttctttac tcggtatcga tgaacctaaa
     5101 gtgaataccc ttaatattga ttcaaattct tttttaagtg gggtagcgaa ctcttatgga
     5161 gaacaatcta ctgaaaaagc cactttttca ggtatttctt ggtataaaga agatgcctta
     5221 aaagaaattt tagattctga acgtaatgtt gttcattttt ggaaacggat gaatcaatta
     5281 aataataaga tatatgttga tttgtctcaa aaaaatgcct tatggattga tatgggaact
     5341 ccagaagggc ttatgaaagc gaatttagct cgcttaaatg aattaggtat tgattcttgg
     5401 gtagatccac ttcatccatt agcaaagcaa aaaaataact tccatcaaag tgtagttcat
     5461 aagcatgcta aagtagctct aggagctgtc ttaaatcgag tcattttatt cgatggagct
     5521 gaagttttag aaaacgaagt cgtttctaat caaattcgtg gtgatggctt tcgctggaat
     5581 ttataatgct tgagtttgtg attgttttag tagagccaga gcaccctcat aatgttggct
     5641 ttgtcgcgag agccatgtgt gcaaatggtt tgactgatct tcgaatcgtt tatcaaaagc
     5701 ataatagcgt attagcagat tcttatagaa cagcacacgc ttcccaatct attcttgatc
     5761 acgcaaaaat cacgaaaaac ttgaaagaag cgattcaaga tgttcaatat acgattgctt
     5821 ttagtcgtcg tacttttgaa agtgtcgttc ctgtgatttc attaccttcg attcaaacca
     5881 ttttaccgaa agaaggtaaa gtggctttag tttttggccg agaatccatt ggccttttat
     5941 tagaagaaat taatcaatgc gctcagatat gcgaaatccc tgttccaggg aatcacagct
     6001 taaatttagg gcaagcggtt tccgtggctt tatatgagct cttaaaagaa aataaatctg
     6061 gtttattaaa tgattattct tctcctgttt caaaggaaaa cagcttagcc aataaagca
//
LOCUS       DAOOBB010000118         6070 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_15166_length_6070_cov_4.096924, whole genome shotgun sequence.
ACCESSION   DAOOBB010000118 DAOOBB010000000
VERSION     DAOOBB010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 6070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6070
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15166_length_6070_cov_4.096924"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="PLT31_06310"
     CDS             <1..1180
                     /locus_tag="PLT31_06310"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HPW94787.1"
                     /translation="NGKFLIVVDDEDRENEGDFIIAAEKVTPEAVNFMLKEGRGVLCA
                     PLSISRCKALGLTRQTQENTSILGTPFTIMVDKIEGCTTGVSAADRAATIQALADPSS
                     VATTFGRPGHISPLYAQEEGVLRRAGHTEAAVDLAKLAGFQPAAALIEIMNEDGSMAR
                     LPDLEKIAKKHNLKLISIRDLIAYRLRTEKLVERVATPSVPTKYGEFKVYGYRSITDG
                     IEHVAWVSGDLDYSKPVYVRVHSECLTGDIFGSRRCDCGEQLDAAMRFIAEHGGVFLY
                     MRGHEGRGIGLCNKLKAYELQQQGLDTVEANLQLGFKSDLREYGIGAQILADIGVRQI
                     RLLTNNPTKVTGIKGYGLEIVERVPIEINPTDDNRFYLETKKNKMGHVLNFSEKEVKS
                     "
     gene            1177..1656
                     /gene="ribH"
                     /locus_tag="PLT31_06315"
     CDS             1177..1656
                     /gene="ribH"
                     /locus_tag="PLT31_06315"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HPW94788.1"
                     /translation="MMNRDVEEIEVSLCGKEARVAIVVSRFNETITEALLKGALYKLN
                     ALGVSSKAITLVRVPGAFEIPGTCRQLVKSNKYDAILTLGAVIRGETPHFDVVVNAVA
                     SGVASLSANSDIPILFGVLTTENVEQAMNRSGLKSGNKGAETAEALVEMINLYKKIK"
     gene            1653..2093
                     /gene="nusB"
                     /locus_tag="PLT31_06320"
     CDS             1653..2093
                     /gene="nusB"
                     /locus_tag="PLT31_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPW94789.1"
                     /translation="MSTISKRPSRVFAMQLLYAMEITAGTPGECLPGVLQSQPIQDDL
                     KKYGMSLVDLVLEHRKELVEVIEGLSHSWKIDRMPILDKIILEIAFIELLYKKEIPVK
                     VIIQEAVQIANKFSTDDSARFINGLLDVYAKSHQMFIDQKSREE"
     gene            2097..5183
                     /locus_tag="PLT31_06325"
     CDS             2097..5183
                     /locus_tag="PLT31_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HPW94790.1"
                     /translation="MLKNEKWIKHLVAGFTALVAFIVYAMTMAPTVSFWDCGEFIACA
                     NTLGIPHPPGTPFFVFFARFVIVILPFVEEIAKRVNYISVVSSAATVYLISLFAWDLL
                     TKVLAKDSLASKLSKTTRYIVLGTAGLVSGFLLTFSDTFWFNAVEAEVYGVAMFILVL
                     VSYLGLVWYDRREKEQGDRILIFICYIAFLGVGAHLYTMLTIPALFVLILIADFNKIK
                     QKTPIWITGVLLCSVIYYVSGFISISLVTLLALLILTLTKPFSVPVMRYVRLAFAFTF
                     VALIGYSTHLYIPIRSELNPIIDENNPEINIRDEQGNLQLGNLLKDENWVAFNNFIER
                     KQYGSESMISRAFYRRAQLSHQILSFPNMGFGGYQIAQYLPFKVGGVNSSRGLYSFDA
                     SENQPQTRLGIDFPTQMTVMGDHTFVQALWFLFFNGLIIAVCVFVYRRHHKLGAYLSV
                     LYVLCSLGLIFYINFADGTRMEKRDYDYWMKAMSRNVTDLNRSGIAISSVPNANALID
                     MRQNIERAKQTVSNLRYNNADPSAIAAAEADVRKYENSSVWQNWRQIENAYARVGQRP
                     PFPEPVHLEVRERDYFYTPAFIFMSLIFGIGAGILVLLVALYQMSLTIPVAAVLTLVA
                     LIVPSVSNYKEHDRSGLWVPWDYAYNLLQSCRPNAILFTNGDNDTFPLWFAQEVAGIR
                     KDVRVVNLSLGNTDWYIKQMLENEPVLKLSYDKEGIDRDMVINDASDNNPNHMTATWI
                     KKANTLIPILEKRIQEMESSDSTQSNVPLDSAKIMQFKVMLQVYTAFKEWQERSGAGL
                     MLTQHKLVIDLAIQNMDRPIHFSTTVGSSNFMGLEKYMVQEGLIYNFIKGDLSPRQNA
                     FDIDRSGYLIDSVYKFRCLGDGSCYINTETERLLSSYTSLYLQIAFNLREQIVEIRKS
                     GPYTVEKKLVVDEKLKQASHYLEKGIYQFPNEWRSYWAAAFVYEAAGQYQKAKEYLEK
                     GLLNVPEYEHQGRGRLSMSIQSMQGVPDATPVMKIESQDSAQDTSISDSSTTKNQE"
     gene            5198..>6070
                     /locus_tag="PLT31_06330"
     CDS             5198..>6070
                     /locus_tag="PLT31_06330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPW94791.1"
                     /translation="MDLSIVIPIKDEADNLADLFAEIVSAIEPTHYSYEVIIIDDGSR
                     DDSWEVLTSLISKYSFIRAYRFQFNCGKAAALSLGFSKAKGHYVATLDGDLQDDPLEI
                     PKMIRILESGYDLVSGWKKRRHDPWHKTWPSKLFNLTVSFVCGQRLHDFNCGIKVYRS
                     SVVRFIPLYGDFHRFIPVMAKWQGFRITEMPVTHRARVHGVSKYGVSRLVSGFLDLLT
                     LLFLKSFGAKPLHFFGLIGLLFVFCGLGITGYFGIEWLQTGALRVRPLLLMGGFSLVM
                     GVQFISLGLLGEMMN"
BASE COUNT         1740 a         1015 c         1253 g         2062 t
ORIGIN      
        1 aaacggaaaa tttctgatcg tcgtggatga tgaagatcgt gaaaatgaag gtgattttat
       61 aatcgcagca gaaaaagtga cgcccgaagc cgttaatttt atgttaaaag aaggtcgtgg
      121 cgtgctttgt gctcctttat ctatctccag atgtaaagct ctaggtttaa ctcgccaaac
      181 acaagaaaat acttctattt tgggaacacc ttttaccatc atggtggata aaattgaagg
      241 ctgtacaaca ggtgtttccg ctgctgatcg tgctgctact attcaagcgc tagctgatcc
      301 ttcttctgtt gcaactacgt ttgggcgtcc agggcatatt tctcctctat acgctcaaga
      361 agaaggtgtt cttcgtcggg caggtcatac agaagccgcc gttgaccttg ccaaattagc
      421 aggttttcaa ccagccgccg ctttgattga gatcatgaat gaagacggct ctatggcgcg
      481 tttaccagat ttagaaaaaa ttgccaaaaa acataatttg aaattaatct ctattcggga
      541 tttaattgct tatagattac gtacagaaaa attagtagaa cgagtggcca ctccaagtgt
      601 gcctaccaaa tatggtgagt tcaaggttta tggctatcgt agtattaccg atggaattga
      661 acatgtcgca tgggtaagtg gtgatttaga ttattcaaaa ccagtttacg tacgtgtgca
      721 tagtgagtgt ttgactggag atatttttgg ctctagacgt tgtgattgcg gcgaacaact
      781 agatgctgca atgcgtttta ttgctgagca tgggggcgtg ttcctttata tgcgaggtca
      841 tgaaggtcgt ggaataggct tatgtaataa attaaaggcc tatgagttac aacaacaagg
      901 tttagatact gtagaagcaa atctccagtt aggatttaaa tcagatttac gtgaatatgg
      961 aattggagcg cagatacttg cagatattgg cgttcgtcaa ataaggcttt taacgaataa
     1021 tccaaccaag gtcactggca taaaaggtta tggtttagaa attgtagaac gggttcctat
     1081 agaaataaac ccgaccgatg ataatcgttt ttatttagaa acaaaaaaga ataaaatggg
     1141 acatgttttg aacttttccg aaaaggaagt aaagtcatga tgaatagaga tgtagaagaa
     1201 atagaagtga gcctttgtgg taaagaggct cgtgtggcaa tagtcgtatc tcgttttaat
     1261 gaaacgatta cagaagcatt gctcaaaggt gctttatata aattaaacgc tttaggtgtt
     1321 tcttcaaaag ctattacatt ggttcgagtt ccaggagctt ttgaaattcc aggcacttgt
     1381 cgtcaattag taaagtcaaa taaatatgat gctattttaa ctttaggtgc tgtgattcgc
     1441 ggtgaaacac ctcactttga tgtcgttgtt aatgccgttg caagtggtgt tgcatctctt
     1501 tcagcaaata gtgatattcc aattcttttt ggcgtattaa ctactgaaaa tgtggaacaa
     1561 gcaatgaatc gctctggttt aaaatcaggg aataaaggtg ctgaaaccgc tgaggctttg
     1621 gtagaaatga ttaaccttta taagaaaatc aaatgagtac tattagtaaa agaccttcac
     1681 gtgtttttgc aatgcagctg ttgtatgcta tggaaattac tgctggtact ccaggagaat
     1741 gtttaccagg tgttcttcaa agtcagccaa tacaagatga tttgaaaaag tatgggatgt
     1801 ctctagtaga tcttgtgctt gaacatcgaa aagaattggt tgaggtgatt gaaggcttat
     1861 ctcatagctg gaaaatagac cgtatgccta ttttggataa aatcattttg gaaatagctt
     1921 tcatcgaact gctttataaa aaagagatcc ctgtaaaggt gatcattcaa gaagctgttc
     1981 aaattgctaa taaatttagc acagatgatt ctgcccgctt tattaatgga cttttagatg
     2041 tgtatgctaa aagtcatcaa atgtttattg atcaaaaatc tcgagaggag taagacgtgc
     2101 ttaaaaacga aaaatggatt aaacatctag tcgctggatt tactgcatta gttgctttta
     2161 ttgtttatgc aatgacaatg gcgccaacgg taagcttttg ggattgtggt gaatttatcg
     2221 cgtgtgcaaa tacgttaggt attcctcatc ctcctggaac tccgttcttt gtcttttttg
     2281 cacgatttgt aatagtgatt cttccttttg tagaagaaat agcaaaaaga gttaattaca
     2341 tttcagttgt gagttctgct gccaccgtgt atttaatttc gctgtttgct tgggacttac
     2401 ttaccaaggt tttagctaag gattctttag cctcaaaact atcaaaaact actcgttata
     2461 ttgttcttgg caccgctggt ttagtctctg gctttttact cacgttctct gatacattct
     2521 ggtttaatgc cgtggaagct gaagtatacg gtgttgccat gtttatcctt gttttggttt
     2581 cttatcttgg tttagtgtgg tatgatcgtc gcgaaaaaga acaaggtgat cgtattttaa
     2641 tttttatttg ttatatcgca ttcttagggg taggcgctca cttatataca atgcttacta
     2701 tcccagctct ttttgtttta attttaatcg ctgattttaa taagattaaa caaaaaacgc
     2761 ctatctggat tacaggtgta cttctttgtt ctgtgattta ttatgtctca ggatttattt
     2821 ctatttcatt agtgacttta cttgcattac tcattttaac tttaacaaaa cctttttctg
     2881 ttccagtgat gcgttatgtg cgactcgctt ttgcttttac atttgtggct ttgattggtt
     2941 atagcacgca tttatatatc cctattcgtt cagagttaaa cccaattatt gatgagaata
     3001 atccagaaat taatatccgt gatgaacagg gaaatttaca acttggtaat ttattaaaag
     3061 atgaaaattg ggttgctttt aataatttta ttgaacgtaa acaatatggc tctgaaagca
     3121 tgattagtag agctttctat cgccgagctc aactttctca tcaaatctta agtttcccaa
     3181 atatgggctt tggtggttat caaattgctc aatatttacc gttcaaagta ggtggtgtaa
     3241 attcatccag gggcctttac tcttttgatg cttccgaaaa tcagccacaa actcgcttgg
     3301 gtatcgattt ccctacacaa atgacggtga tgggagatca tacttttgtt caagctcttt
     3361 ggtttttatt ctttaatggc ttaattattg ctgtatgcgt ttttgtttat cgcagacatc
     3421 acaaattggg tgcatacctt tctgtgctct atgttctttg ctctttaggc ttgatatttt
     3481 atatcaactt tgccgatggc accagaatgg aaaaacgaga ttatgactat tggatgaaag
     3541 ccatgtctcg taatgtaacg gatcttaacc gaagcggaat cgctatcagt tctgtgccca
     3601 atgcaaatgc tttgattgat atgcgtcaga atattgaacg tgcaaaacag acggtttcta
     3661 atcttcgcta taataacgca gatccatctg ctattgcggc ggcagaagct gatgtacgta
     3721 agtatgaaaa ttcatctgta tggcaaaact ggagacaaat cgaaaacgct tatgctcgtg
     3781 tggggcaacg tcctccattc cctgagcctg ttcatttaga agttcgcgaa cgtgattatt
     3841 tctatacacc agcatttatt ttcatgtctc tcatttttgg tattggtgca ggtattttag
     3901 ttttactcgt tgctctttat caaatgtctc ttacaattcc tgtagcagct gttttaacgc
     3961 tagtggccct tatcgttcct tccgtttcaa actataagga acatgatcgt tcaggtcttt
     4021 gggttccatg ggattatgct tataacttgt tacagagttg taggccaaat gccattttat
     4081 ttacaaatgg tgataatgat acgttccctc tttggtttgc tcaagaagtg gctggtattc
     4141 gtaaagacgt gcgtgttgtc aatttaagtc ttggtaatac agattggtac atcaagcaga
     4201 tgcttgaaaa tgaaccagtt ttaaaattaa gttatgataa agaaggcatt gatcgtgaca
     4261 tggtcattaa tgatgcttcc gataataacc ctaatcacat gactgctaca tggattaaaa
     4321 aagcgaatac tttgatccct attcttgaaa agagaattca agaaatggaa tcttctgatt
     4381 ctactcaatc aaatgtccct ttagattccg cgaaaataat gcaatttaaa gtgatgcttc
     4441 aagtttatac ggcctttaaa gaatggcaag aacgttctgg agctggctta atgttaactc
     4501 aacataaatt agtcatagac cttgctattc aaaatatgga tcgtccaatt catttttcaa
     4561 cgaccgtggg ttcttcgaat tttatgggac ttgaaaaata tatggtacaa gaaggcctaa
     4621 tttataattt cattaagggt gatttatctc ctcgtcaaaa tgcttttgat atagatcgtt
     4681 caggttatct cattgatagc gtgtataagt tccgctgtct tggcgatggc tcatgctata
     4741 ttaatacaga aacagaacgt ttgttatcta gctatacttc tttatacttg caaattgcct
     4801 ttaatttaag agagcaaatt gtagagatta gaaagtcggg cccttataca gtcgagaaga
     4861 agttggtggt tgacgaaaaa ctcaagcaag cttctcatta tttagaaaaa ggaatttatc
     4921 agttccctaa tgagtggcgt tcttattggg ctgcagcttt tgtttatgaa gcagcaggtc
     4981 aataccaaaa agcaaaggaa tacttagaaa aaggacttct aaatgttcct gaatatgaac
     5041 atcaaggtcg tggacgttta tctatgagca ttcagtcaat gcaaggtgtt ccagatgcga
     5101 ctcctgttat gaaaatagaa tcacaagact ctgctcaaga tacgtctatt tctgattctt
     5161 caacaacaaa gaatcaagaa taataaatga gataaccatg gatttaagta ttgttatacc
     5221 tattaaagac gaagcagata atcttgctga tttatttgct gaaatcgtat cagcgataga
     5281 gcccactcat tattcttatg aagtgatcat tattgatgat ggaagtcgag atgattcttg
     5341 ggaagtcttg acctcattaa taagtaaata ttcttttatc cgtgcgtatc gttttcaatt
     5401 taactgtgga aaagcggcag cactttcctt aggcttttca aaggcgaaag ggcattatgt
     5461 cgctactctt gatggtgatt tacaagatga ccctttagaa attcctaaaa tgattcgtat
     5521 tttggaaagt ggttatgatt tggtttctgg ttggaaaaaa cgtcgtcatg atccatggca
     5581 taaaacatgg ccatctaaac tgtttaattt gactgtatca tttgtttgtg gtcagcgtct
     5641 ccatgatttt aattgtggta ttaaagttta ccgaagttct gtagtacgtt tcatcccatt
     5701 atatggtgat tttcatcgat ttattcctgt tatggcgaag tggcagggtt tccgaattac
     5761 tgaaatgcca gtgactcata gagccagagt ccatggtgtt tcaaaatacg gtgtttctcg
     5821 tttggtttca ggttttttag acttattgac attattattc ttaaaaagtt tcggtgcaaa
     5881 gccccttcac ttttttggtc tgatcggttt actttttgta ttttgtggcc ttggtattac
     5941 aggctatttt ggaatcgagt ggcttcaaac aggagctctt cgtgttcgtc cgttactact
     6001 catgggtggt ttttctcttg taatgggcgt tcagtttatt tctcttggct tattaggaga
     6061 gatgatgaac
//
LOCUS       DAOOBB010000119         5951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_15508_length_5951_cov_5.115672, whole genome shotgun sequence.
ACCESSION   DAOOBB010000119 DAOOBB010000000
VERSION     DAOOBB010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 5951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5951
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15508_length_5951_cov_5.115672"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1998)
                     /locus_tag="PLT31_06335"
     CDS             complement(<1..1998)
                     /locus_tag="PLT31_06335"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPW94792.1"
                     /translation="MKINQAISLFVLILSLVYIGCDSSNNTTPIEESPLEVISTVDNL
                     PKCDNKNQGETHIVGHEETLYFCFNETWQTLEDTTHFSVTCKDGYIEVISKATDSNNN
                     SNPDFNFIGPTFTLDFYGYAQKGPFMIGSSIQVTELDSSQFYRPSQRSFDGCLFSKNG
                     RFEFTNVQVLSRYLQVSASGFYRNEITGERSQKTLTLKSIVDMNNRDSVNINVLTHLE
                     SGRLEQLIATSGGYLPINQAKEQAAKEVLKAFEIDIQNWNNQSISQNTMHVEDFNLFG
                     NSDSSAALLAISIMLQGNRSEDEVANVINQISDDLKGDGYWNDMNLKTKIADWLMVQD
                     TSWQYNSIRNNISQWNFGPIPNFEKHLRNFWSKTYGFEACNESTAGQVKYVNNNSSAF
                     YASYYSHIDNTKLRFICDASSKMWRAARDIEKDTIGFGPGKYDKEIREGLVNTGNFYL
                     YEQSNLAWRLATPQEADGFTDILEVYSQLAADEKVFFVIRHAERTNDTGKDGKLTDNG
                     VNQAKKLGASLVGEDFFYASSDFFRASETCRNIAVGKGQSNYKIETWEALSGEWYILN
                     SSKLESYKNSNGGGWQVFSKWAFLNEYSDAFYDLEKRSEELLEDYVFANLSKVSRAGV
                     LCSHDQLVVPLLAYTTNKHANVRYYENNRWLNYLSGVAIIIN"
     gene            complement(2153..3004)
                     /locus_tag="PLT31_06340"
     CDS             complement(2153..3004)
                     /locus_tag="PLT31_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02147 family protein"
                     /protein_id="HPW94793.1"
                     /translation="MNEQKKNLKIYEYLDYREFLQDYYNFKKASSNSFSLRVFSDKIG
                     FKAKDFISRVMNGEKNLSPSSASKIASGLNLSVRERSFFEALVLFNQADSTKERDNAF
                     AQIQKILKLVRFKEKQHLMAFYQYEVFSHWRHLVIRSLIGMFGFSGDYESLAKQVQPK
                     VTPDETKKSVELLEKCNLIQKKSNGEYFLTENAITTGNKTSKLALRGFHQHCLRLAID
                     SIDREEPSKRNISALTLGISKNSYDQIVEKINEFRKEIALLAEEDENADSVYQMNFQL
                     FPVGGKK"
     gene            3184..4842
                     /locus_tag="PLT31_06345"
     CDS             3184..4842
                     /locus_tag="PLT31_06345"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94794.1"
                     /translation="MAYRTQRSCLINRAWVYILLIFLTCLGCSDQHAGGNSAESGNPE
                     LAGILMLKGKEPAGLARVQCVPSDFNVMYDTLPLSFKTKTDSLGHFSFDSLPKGEFSL
                     EAYHQASGERLLIQNLEITEDSVIEENLLPTGVLRIGVEDLEENDSGFVILQGTTLYR
                     PVTVKYGTLFVDSLPVADYDSLVYVPFGESLENGFTLARDVTIASDDTLKINASLLSL
                     SLIAPLKYPDGDTLSSIIRDIPLAFRIDSTLIDFDVLSRLSGRWTAMRGDFDLPISLA
                     RYDVISKEIVFWIRVDSLLAEDTLSLHFNEGLSTIYAKDVFPESAGYIAVWHFDEGDS
                     LVEDATELKQFPGVSNDVREEPGSVGKSFFYNGKSSYVSIPNSASGDLNFAIDDTMSI
                     SVWVRLDTLNTSRFVYGKGATQYHLKYLYPTGWLFEEYEEAETGTTRYWYLPDTTKET
                     SKDWTYITITMRGQETNLYINDSLITKEPKIGGFESERYTENLFEIGRQVFPLSSDSS
                     GQYFYGAIDELHVSFVSRKPDWIKASYWNQRPTNYWPRLKNKKQ"
     gene            complement(4921..5457)
                     /locus_tag="PLT31_06350"
     CDS             complement(4921..5457)
                     /locus_tag="PLT31_06350"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPW94795.1"
                     /translation="MKSKKKCGKVDILVNCAGSAENAGVIDMTDEQWDFTIKTDLTSV
                     FFVTRAFANIMKKNNYGRIINIASMYGLVGNTAIDTVAYHSSKGGVVNFTRAVAAELA
                     KYQITCNAICPGYFETELTRDVLKTESFKKYIDQTVPLKRHGNEGELNAAAIFLASDE
                     ASYVTGAILPVDGGYTAV"
     gene            complement(5668..>5951)
                     /locus_tag="PLT31_06355"
     CDS             complement(5668..>5951)
                     /locus_tag="PLT31_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94796.1"
                     /translation="LWDGITRISNIKNSNKTFTYNIHSGVIQSAQDGIMEVNVFDLQG
                     KRILSKKLSIMQGTTMFSLEKENLSLGSYLVQIRLNHKNVSIFKWTQMK"
BASE COUNT         1864 a         1045 c         1062 g         1980 t
ORIGIN      
        1 attgataatg atggcgactc cagaaaggta atttaaccat cgattatttt cgtaatacct
       61 tacattagcg tgtttatttg ttgtataagc aagcaaagga acaactaatt gatcgtgaga
      121 gcataacacc ccagcgcgac tcactttaga aagatttgca aaaacatagt cttcaagaag
      181 ctcttcactg cgtttttcta aatcataaaa tgcatcagaa tattcattta agaaagccca
      241 tttagaaaag acttgccagc ctccgccatt agaattttta taggattcta gcttagaact
      301 attcaatata taccattcgc cagataacgc ttcccacgtt tctattttat aattgctttg
      361 cccttttcca acggcaatat tcctacaagt ttcagaagct ctaaaaaaat cagaagaagc
      421 gtagaagaaa tcttcgccta caagagatgc tcctaatttc tttgcttgat taacgccatt
      481 atcggttaac ttgccatcct ttcctgtatc atttgttctt tctgcatgcc ttatcacaaa
      541 aaagactttt tcatcggctg ctagttgaga gtacacttct agaatatcgg taaaaccatc
      601 tgcttcttgt ggggtagcaa gacgccatgc taaattggac tgttcataga gatagaagtt
      661 tccagtatta acgagccctt cgcggatttc tttatcgtat tttccaggcc caaaaccaat
      721 cgtatctttt tcaatatctc tagcagctcg ccacatttta gaagaggcgt cgcaaataaa
      781 acgaagtttg gtattgtcta tatgagaata gtaagaagca taaaaggcgc tactgttgtt
      841 gttgacatat ttcacttgcc ctgcagtgga ttcattgcaa gcctcaaatc cgtaggtttt
      901 agaccaaaag tttcgaaggt gtttttcaaa attaggaatc gggccaaagt tccattgcga
      961 gatattgttt cgaatggaat tatattgcca gctagtgtct tgaaccataa gccaatcggc
     1021 gattttcgtt ttcaaattca tatcgttcca atagccatcg ccttttaaat catcgctgat
     1081 ttgatttatt acattagcga cttcatcttc agaacggttc ccttgtaaca tgatagaaat
     1141 ggctaaaaga gctgcacttg aatcgctatt tccaaaaaga ttaaagtctt ctacatgcat
     1201 tgtattttgg ctaatactct gattattcca attctggatg tcaatttcaa aagcttttaa
     1261 aacttctttt gctgcttgtt cttttgcttg attaatgggt aggtatccac cactagtcgc
     1321 tatcaattgt tctaatcgtc cagattctaa atgggtgagt acattgatat tcaccgagtc
     1381 gcgattgttc atgtccacaa ttgattttaa ggtaagtgtt ttttgggaac gctcaccagt
     1441 gatttcattg cgataaaaac cactagcaga tacttgtaaa taacgggaaa ggacttgaac
     1501 attggtgaat tcaaatcgcc cgttctttga aaaaagacaa ccgtcaaaag atctttgact
     1561 aggtctataa aactgagagc tatctagttc agtaacttga atagaagaac caatcataaa
     1621 aggccctttt tgagcataac cgtaaaaatc taatgtaaaa gtagggccta tgaaattaaa
     1681 atcaggattt gaattgttat tagaatcggt tgcttttgaa atgacttcta tatagccatc
     1741 tttacaggtg acgctaaaat gagtggtgtc ttctagtgtt tgccaagttt cattaaaaca
     1801 aaaataaaga gtttcttcgt gccctacaat atgggtttca ccctgatttt tattatcgca
     1861 tttaggaaga ttatcaacag tagaaattac ttctaatgga gattcttcaa ttggcgttgt
     1921 gttattagaa gaatcacagc ctatgtaaac taggctcaaa ataagaacaa aaagggagat
     1981 ggcttgattt atcttcattc aaaatacttt gtaactaggg gttcttcttt ttcactaata
     2041 gtgattcaaa tatacaaaat atattatgtg taaaaaaaag gaatagaatg ctcttattcc
     2101 ctcgcaaaat aggtctttta agatgattag gaacaataaa atgatgaatc aattatttct
     2161 tcccaccaac agggaaaagt tggaaattca tttgatagac agagtctgca ttttcgtctt
     2221 cttcagcaag taaagcgatt tctttgcgaa actcattaat cttttccaca atttgatcgt
     2281 aagaattttt tgaaattcct agagtcaagg cagagatatt cctcttagaa ggttcttcgc
     2341 gatcaattga atctatcgct aatcttaaac aatgctgatg aaaaccacgt aaagcaagtt
     2401 ttgatgtttt attccctgtg gtaatggcgt tttcagttaa aaagtattcc ccgttcgatt
     2461 tcttttgaat taggttacac ttttctaata gttccaccga ttttttagtc tcatcaggag
     2521 tcaccttagg ctgcacttgt tttgcaagag attcgtaatc tccagaaaat ccaaacatgc
     2581 cgattaaaga tcgaatgacc aaatgacgcc aatgagaaaa aacttcatat tgataaaaag
     2641 ccatcaaatg ttgtttttct ttaaaacgga ccaatttaag tattttttga atttgggcaa
     2701 aggcgttgtc tcgttcttta gtagaatcag cttgattgaa taaaaccaga gcttcaaaaa
     2761 aagaacgttc tctaacagat aaattcaatc ctgaagcaat tttagaagca ctagatggcg
     2821 ataagttttt ttccccgttc ataacgcgcg aaataaaatc tttagcctta aagccgattt
     2881 tatcagaaaa aacccttagc gagaacgaat ttgaagaggc tttcttaaaa ttataataat
     2941 cttgcaagaa ttccctgtag tctaggtatt catagatttt aagatttttc ttttgctcgt
     3001 tcatactaat aatttaatta ttctaatcgt tcgaaggaat actatgttta tttagtgttg
     3061 tctaaaaaca actcattttg tgttgcccat agacaacagg aaatagaacg aattgcaaaa
     3121 atggtctata taaatatata tttaatcagt gtaaatatat gtttagttag tccttaaaat
     3181 cttttggctt atagaactca aaggagttgt cttattaatc gggcttgggt ttatatacta
     3241 cttatttttc ttacttgcct aggttgttct gatcaacatg caggtggaaa cagtgctgaa
     3301 tcaggtaatc ctgaattggc tggtatttta atgctaaaag gaaaagaacc agctggttta
     3361 gcacgtgtac aatgtgtccc ttctgatttt aatgtcatgt acgacacttt acctttgtct
     3421 tttaagacaa aaacagattc tttgggtcat ttttcttttg attctttacc taaaggagag
     3481 ttttctcttg aagcatatca tcaggcttcg ggcgagcgtc tattaattca aaatttagag
     3541 atcaccgagg attcagtcat cgaagaaaat cttttgccaa caggcgttct tcgtataggt
     3601 gtagaagatt tagaagaaaa tgatagcggt ttcgttattt tacaagggac tacattatat
     3661 aggccagtga ccgtaaaata cgggacttta tttgtggatt ccttacctgt cgctgattat
     3721 gattccttgg tatatgtccc ctttggagaa tctctagaaa atgggtttac tcttgctcga
     3781 gatgtaacta ttgcatcaga tgatacttta aaaatcaatg catctttact ttctttaagt
     3841 ttaattgctc ctcttaaata tcctgatggg gatacgttaa gttctattat aagagatatc
     3901 ccacttgcat ttcgaataga ttccacttta attgattttg atgtactcag tcgcttgagt
     3961 ggtcgttgga ctgcaatgcg tggggatttt gacttaccca tttctttagc tcgatatgac
     4021 gtgatttcta aagaaattgt tttttggatt cgagtggatt ctttacttgc agaggatact
     4081 ctttctcttc attttaatga aggtctatcc actatttatg caaaagatgt tttccctgaa
     4141 tctgctggct atatagccgt atggcatttt gatgaagggg attctttagt tgaagatgct
     4201 actgaattaa aacaattccc aggggtttcc aatgatgttc gtgaagaacc aggttctgtt
     4261 ggaaaatcgt ttttttataa tggaaaaagt agttatgtca gtattccaaa ttcggcttca
     4321 ggcgacttaa attttgcaat agacgatacg atgagtattt ctgtctgggt tcgtttagat
     4381 actttaaata caagtcgctt tgtttatggc aaaggggcga ctcaatacca tctaaaatac
     4441 ttatatccca caggatggtt gtttgaggaa tacgaagaag cagaaacggg aaccactcgt
     4501 tattggtatc ttccagatac aacaaaggaa acttcaaaag attggactta tattacaatc
     4561 acaatgagag gccaagaaac caacttgtat atcaacgatt ctttaataac aaaagagcct
     4621 aaaattggtg gatttgaatc cgaacgatat acagaaaatc tatttgagat agggcgtcaa
     4681 gtttttcctt taagttcaga tagctctggg caatatttct atggggctat cgatgaattg
     4741 catgttagtt ttgtttctcg taaacccgat tggattaaag catcctattg gaatcaacgc
     4801 cccacaaatt attggccacg tttaaagaac aaaaagcaat agattactgg ccatgtataa
     4861 aagtaggcat taaaaaacct cttgttataa aaacaagagg cctttttttg aatatgaata
     4921 ttaaactgcg gtgtagccgc cgtccacagg caaaatggca cctgtgacat aagaagcttc
     4981 atcactagct aaaaagattg ctgccgcatt gagttcacct tcatttccat gtcgttttaa
     5041 gggaacagtt tggtcaatat attttttgaa agattctgtt tttaaaacat cacgagtcaa
     5101 ttcggtttca aaatagcctg ggcaaatagc attacaagta atttgatatt tcgcaagttc
     5161 tgcagctacg gctcttgtaa aattaacaac gcctcctttg gaagagtgat aagctacagt
     5221 atcaatagca gtattaccga ctaaaccata catggaagca atattgataa ttcggccata
     5281 attattcttt ttcatgatgt ttgcaaaggc acgagttaca aagaaaacgc ttgttaaatc
     5341 ggttttaatg gtaaagtccc attgttcatc agtcatgtct ataacgccag cattttcagc
     5401 agagccagca caattgacta gaatgtcgac ttttccacat ttttttttcg atttcaagag
     5461 ccatttgagc ccctaatcca gatgaggctc cagaaacaac cacatttctt cccttgagat
     5521 taaacatata gctcctctta aatcaaaaaa tagctgaatt gctagatact tataaactac
     5581 gaaaaaacgt cgttgtgcgc tataattaaa taaaaaaagc caacctttag aaaggttgac
     5641 ctttgagaga aatgaataaa tttgaggcta tttcatttgg gtccatttga aaatagaaac
     5701 attcttatga ttcaacctaa tttgaaccaa ataagatcct aatgataaat tctctttttc
     5761 aagtgagaac attgttgttc cctgcataat agagagtttt ttggataaaa ttcgttttcc
     5821 ttgcaagtca aagacattga cttccataat tccatcttga gcggattgaa ttactcctga
     5881 gtggatatta taagtgaatg ttttattgga gttctttatg tttgaaatac gtgttattcc
     5941 atcccataaa a
//
LOCUS       DAOOBB010000120         5911 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_15634_length_5911_cov_4.639344, whole genome shotgun sequence.
ACCESSION   DAOOBB010000120 DAOOBB010000000
VERSION     DAOOBB010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15634_length_5911_cov_4.639344"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            322..2553
                     /locus_tag="PLT31_06360"
     CDS             322..2553
                     /locus_tag="PLT31_06360"
                     /inference="COORDINATES: protein motif:HMM:NF015387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HPW94797.1"
                     /translation="MGDCVVLCNKIKLLVLFAFMALSYAQIPLNYSVENRGQDLSVTA
                     GALKQNNYLPNPFEFTDGSYVTTFDDWSKRRNEIKADIEKYEIGAKPKPPTNLKATYS
                     GGTLTVTVTENGKTVTLTSKFSIPSGAGPHPIVIGMNSGTGSLSTSLFSGVVQVPFNH
                     DQVATYDMTGNKDLNAPFYKMYTNLSSAGDYTAWSWGVSRIIDGLALIADEYNLDMGR
                     IATTGCSYAGKMALFSGAFDERVTLVIAQESGGGGINSWRASDAYAKRVEDVEKINNT
                     NGSWFMKSMLSLNPYQLPHDHHELIAMIAPRAFLALGNPDYVWLGDESGYKSSMAAYE
                     VWKAMGIEDRFGFDFAKGHSHCQASDSQNKAVTAFVDKFLRGKESTDTDIKRNPSNGN
                     FKLDYESLIDWTTPKLESDPTIPKVTLTSPVSGATFEAPATVTITATATDSDGSVAKV
                     EFFNGDKLLASITKAPYTYEWTNVALGSYSIKAVVTDNSGKSSSDGRTVVVSPPQTAY
                     KGVVSKIPGKIEFENYDVCGNGCAYFDDSEGNDGGASFRTDEDVDIEDCTDDGAGYNL
                     GWATAGEWVEYTVDVESPGIYDLVVRAASENANTLSMTVDGKEIVKPIAIPVTGGWQK
                     WQDVKVAGVNLNAGRQVIRVTFGAKYVNLNYMEFKLNTISLAQKLETQMQMHFSNNRI
                     NLEGAPAGAKIRLFDLQGQFLGELGEHGGELPNLKSGKVLMTVVNKNGALLLTKVIPY
                     QAN"
     gene            2563..4293
                     /locus_tag="PLT31_06365"
     CDS             2563..4293
                     /locus_tag="PLT31_06365"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HPW94798.1"
                     /translation="MIPLFLFLCICFLSMTYGQDPNFHIYLAFGQSNMSGQADPTAED
                     KTPDPRFFVLRAAKHSGQTVGQFYPGIPPMGHSQSKMGIADFFGRKMVQELPASIKIG
                     IANVAIGGQSISLFDKATSASYISKARSANEWWIQYLDEYGGDPYKRIIEMGEIAKKE
                     GVIKGILFHQGEADYQMADWPSRVKKVYDDIIADLKLDPTQVPILIGELATTAAGGDL
                     GYRNDAVAEAANMIPNGHLISAADCPALKEASYTLHFTRAGYQTFGERYAQKMLELLG
                     SLDGPVVELSVTSNEVSVGEQVSLEVNATATKGTIVKLELKEGDILLEEKSVVPYTYI
                     LKSLSLGSHQITAIATDSEGNQGQASITIDVFPVQTPYQGVAHAIPGKVEFENYDDCG
                     NGCAYSDDSEGNDGGASFRTEEDVDLEDCTDEGAGYNLGWATAGEWVEYTVDVKNPGT
                     YNLVIRAASENANTISITSDGEELVKGIAIPTTGGWQQWQDVTVKDVVLKQGIQVLRF
                     IIGTDYVNLNYIDFVPISLAIQANNQILNNTSPQTRLFYNQEKQSVFIRIENNGKVEF
                     FDMKGNRVFF"
     gene            complement(4327..5829)
                     /locus_tag="PLT31_06370"
     CDS             complement(4327..5829)
                     /locus_tag="PLT31_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPW94799.1"
                     /translation="MRITFLNPPFYPMFSRESRSPCVTKSSTLYWPMFLSYAAGNVEA
                     DQNEIQLIDSPAMDLDLEATVAKIREFSPELIICSTSTPSILNDLQVIKTLKVLFGVP
                     TAIMGTHATAEPLECLKMEPNLDYCIIGEADHTARQLVRYLRGEIESIKSIPGLAFRD
                     KDGSIDFQPEGPKIENLDEIPWVSKTYYKHLFSCYKRYFYGANINPLIVILTGRGCPH
                     RCSYCVIPQTLNGHVYRRRSPKDVVDELEFIQEHFEDLGEVFFEDDTFTANHDHVKEI
                     CNIILERDLKITWSCNARADVPESLLLLMKKAGCREMCVGFESASPVVLQNIHKGIKN
                     TEKAIEFTKNARKAKILVHGCFMVGNPGDTQETLEMTLQYAKKLNPNTAQFYPIMAYP
                     GTEAYREALDNGALESSDYNQWLDKDGFHRTTIRRGNLSSQNLVDFCDRARREFYLRP
                     SYMFTQAKMALCNSRERYRVLRGFGTLVKHLFRKHGELKSHVRQAPTEKQ"
BASE COUNT         1783 a         1022 c         1270 g         1836 t
ORIGIN      
        1 ggtttactgg tcgaatggaa ggcatcgctt ctcaagcggt tgcttctgtt tatggctctt
       61 tgaacgatca ataattctta tatttaattc tttgtttttt ttaaaattag gcctattttg
      121 ggcctttttt attatgtaat accacctttt gttgtttcaa cttacaacgt cttgttttat
      181 tgaaagataa tacaacatca ttttttgttt actttagaat ctgggtgtgt aaaataacag
      241 aggacttcct taaaatgttt ttggtgagag accaagaact atttatttaa ggatggcctt
      301 ataaacagat aaggagtcat gatgggtgac tgtgttgtgt tgtgtaataa aataaaacta
      361 ttagttttat ttgcctttat ggcattaagc tatgctcaaa ttccattaaa ttatagtgtc
      421 gaaaatcgag ggcaagattt aagcgttacg gcgggagcct taaagcaaaa taattatttg
      481 cctaatccat ttgaatttac agatggaagc tatgtcacta cttttgatga ttggagtaaa
      541 cgaagaaatg aaattaaggc tgatattgaa aaatatgaaa taggggctaa acctaagcct
      601 cctacaaatc taaaagcaac ttattctggt ggtacattga ctgttaccgt tacagaaaat
      661 ggaaaaacag ttacgttaac gtctaagttt tctattcctt ctggagcagg gccacatcct
      721 attgtgattg gtatgaatag cggaacaggt tctctttcga cttctctttt tagtggtgta
      781 gttcaagttc catttaatca cgatcaagtg gcaacttatg acatgactgg taataaagat
      841 ttaaatgcgc ctttttataa aatgtacacg aatttatcgt cggcagggga ttatactgct
      901 tggtcatggg gcgtgagccg tataatagat ggtcttgctt taattgccga tgagtataat
      961 ttggatatgg gccgtatcgc tacaactggt tgctcttatg caggtaaaat ggctttgttc
     1021 tctggggctt ttgatgaacg agtcacttta gtgatagctc aagaatcagg tggaggtggc
     1081 attaactctt ggcgtgcttc tgatgcttat gcaaagcgcg tagaagatgt agaaaaaata
     1141 aataacacga atggaagctg gtttatgaaa agcatgcttt ctttgaatcc ttatcaattg
     1201 cctcatgatc atcacgagtt gatagcaatg attgccccta gagccttttt agctttgggt
     1261 aatccagact acgtttggtt aggtgatgag tctggttata aatcatcaat ggctgcttat
     1321 gaagtttgga aagccatggg tatagaagat cgctttggat ttgactttgc gaaagggcat
     1381 tctcattgtc aagcgtcaga tagtcaaaat aaagctgtga ctgcttttgt agacaagttc
     1441 cttcgaggga aagaatcaac tgatacagat attaaaagaa atccatcgaa cggtaatttt
     1501 aaattagatt acgaatcgtt gattgattgg acaactccaa agctcgaatc cgatccaaca
     1561 atacctaaag tcactcttac ttctcctgta tcgggagcca cttttgaagc tcctgcaaca
     1621 gtgactatta ctgctacggc aactgattct gatggtagtg tggcaaaagt cgaattcttt
     1681 aatggagata agcttctggc gtcaatcaca aaggctcctt atacttatga atggactaat
     1741 gtggctttag gttcttatag tataaaggct gtagtgactg ataatagtgg aaagtcaagc
     1801 tcagatggaa gaaccgttgt tgtttctcct ccacaaacag catataaggg cgtggtttca
     1861 aaaattcctg gtaagattga gtttgaaaat tatgacgtgt gtggtaatgg ctgtgcctat
     1921 tttgatgatt ctgaagggaa tgatggtgga gctagtttcc gtacagatga agatgtagat
     1981 attgaagact gtaccgatga tggagctggt tataatttag gttgggccac cgcaggagaa
     2041 tgggtggagt atactgtaga tgtagaaagc cctggcattt atgatcttgt tgttcgagcg
     2101 gcaagtgaaa atgcaaatac tctttctatg actgtggatg gaaaagaaat tgtaaaacca
     2161 atcgctattc cagttacagg tggatggcaa aagtggcaag atgtaaaagt agctggtgtt
     2221 aatctaaacg cgggtcgaca agtgattcgt gtgacttttg gtgcaaaata tgtgaactta
     2281 aactacatgg aatttaagtt aaacaccatt tcattggctc aaaaattaga aactcaaatg
     2341 caaatgcact tctccaataa cagaatcaat cttgagggcg cacctgctgg agccaaaatc
     2401 cgtctttttg atcttcaagg ccaattcctt ggtgagcttg gagaacatgg tggtgagtta
     2461 cctaatctaa aatctggaaa ggtgcttatg actgtagtaa ataaaaacgg agctttgtta
     2521 ctaacaaaag tgattccata ccaagcgaat taaagagaac gaatgatacc tctgttttta
     2581 ttcttgtgta tttgcttctt gtccatgact tatggacaag atccaaattt ccatatttac
     2641 ttagcctttg ggcaatcgaa tatgtcgggg caagcagatc ccactgctga agataaaacg
     2701 cctgatcctc ggttttttgt gcttcgtgca gccaagcatt cagggcaaac ggtagggcaa
     2761 ttttacccag gaattcctcc tatgggtcat agccaatcga agatgggaat cgctgacttt
     2821 tttgggcgta aaatggtcca agaattacca gcatccataa aaatagggat tgccaatgtt
     2881 gctattggag ggcaaagcat tagcttgttt gacaaagcga caagtgcatc ttatatcagc
     2941 aaggctcgta gtgctaatga atggtggatt caatatttag atgaatatgg tggagatccc
     3001 tacaaacgca ttattgagat gggagaaata gcgaaaaaag aaggtgtcat taaagggatt
     3061 cttttccatc agggcgaagc ggattatcaa atggcagatt ggcctagtcg tgtaaagaaa
     3121 gtctatgacg atattattgc agacttgaaa cttgacccaa ctcaggtgcc gattttaatt
     3181 ggagaactcg ccaccacagc ggcgggcggt gatttaggtt atcgtaatga tgctgttgct
     3241 gaagcggcaa acatgattcc taatgggcat ttaatttctg ctgctgattg ccctgcttta
     3301 aaagaggcta gttatacact tcattttacg cgtgctgggt atcaaacatt tggtgaacgt
     3361 tacgctcaaa aaatgcttga actattaggc tctttagacg gacctgttgt cgaattaagt
     3421 gttacttcta atgaagtttc tgttggcgaa caggtctcgt tagaagtcaa tgcaaccgcg
     3481 acaaaaggaa ctattgttaa actagaactc aaagaagggg atattctttt agaagaaaaa
     3541 tctgttgttc cttatacgta tattttaaag tctctttctt tagggagtca tcaaattact
     3601 gccatcgcca cagatagtga agggaatcag gggcaagctt ctattacaat agatgttttt
     3661 cctgtacaaa caccatacca aggcgtagcg catgctattc ctggaaaagt ggagtttgaa
     3721 aattatgacg actgtggtaa tggttgtgct tattcagatg attctgaagg gaatgatggt
     3781 ggtgctagtt tccgcacaga agaagatgtg gatttagaag attgtacaga tgaaggggct
     3841 ggttataatt taggctgggc cactgctggt gaatgggtag agtacactgt ggacgtgaaa
     3901 aatcctggga cttataatct agtgattcga gcggcaagtg aaaatgcaaa tacgatttca
     3961 attacttctg atggagaaga attagtgaaa ggtattgcta ttccaactac aggaggatgg
     4021 caacagtggc aagatgtgac agtaaaggat gtcgtgttaa aacaaggcat tcaggtgctt
     4081 cgattcatca ttggcacaga ttatgtgaac ttaaattaca ttgattttgt tccaatttct
     4141 cttgcaatac aagcaaacaa tcaaatccta aataacacct ctcctcaaac tcgtttattc
     4201 tataatcaag aaaagcaaag tgtctttatt cgcatagaaa ataatgggaa ggtcgagttc
     4261 tttgatatga aagggaatag ggtattcttt taacaaaaaa ccgcgagaca agctcgcggt
     4321 ataatcttat tgcttttctg taggcgcttg tcgaacatga ctttttagtt cgccgtgttt
     4381 tctaaagaga tgtttaacta atgtgccaaa acctcttagt acgcgataac gttcacgact
     4441 attgcataaa gccatttttg cttgagtaaa catataggaa ggacgaagat aaaattctcg
     4501 ccttgccctg tcacagaaat cgactaaatt ctgactgctt aaattgcctc ggcgaatcgt
     4561 tgttctgtgg aaaccatctt tatcaagcca ttgattataa tcagaacttt ctaatgctcc
     4621 attatcgaga gcttccctat aagcttcggt accaggatag gccataatag gataaaattg
     4681 agctgtattc ggatttaact ttttagcata ttgaagagtc atttcaagag tttcttgtgt
     4741 gtctcctgga ttaccaacca taaaacaacc atgtactaaa atttttgctt ttcttgcatt
     4801 tttagtaaac tcaattgcct tttcggtatt cttaattccc ttatggatat tttgaagaac
     4861 aacaggactt gcgctttcaa aacccacaca catttctcga catcctgctt ttttcattaa
     4921 cagaagtaat gactctggca catcggcacg cgcattacaa ctccaggtga ttttaaggtc
     4981 acgctctaga atgatgttac atatttcttt aacatgatca tgattagctg taaaagtatc
     5041 gtcttcaaaa aagacttccc ctaaatcctc aaagtgttct tgaataaatt caagttcgtc
     5101 tacgacatct tttggacttc ttcttctgta aacatgcccg tttaatgttt gcggaattac
     5161 acaatagcta cagcgatgag gacaacctct tccagtgaga atcacaatca aggggttaat
     5221 attggcgcca taaaaataac gtttgtaaca agagaatagg tgcttgtaat aagttttact
     5281 aacccaagga atttcatcta ggttttcaat ctttggacct tcaggttgaa agtcaattga
     5341 gccatcttta tctcgaaatg caagacctgg aatggatttg atcgattcaa tttcaccacg
     5401 tagatagcgt actagttggc gagctgtatg atccgcttct ccaataatac agtagtctaa
     5461 gttcggctcc atctttaagc attcaagagg ttctgctgtg gcgtgcgttc ccataatggc
     5521 ggtcggcacc ccaaataaaa cttttagggt tttaatcact tgaagatcat ttaaaatact
     5581 tggggtcgat gtgctacaga taattaattc aggtgaaaat tcacgaattt tggcaactgt
     5641 agcctcaaga tcaagatcca tagctgggct atcgatgagt tgaatttcat tttggtcagc
     5701 ttctacgttt ccagcggcat agcttaagaa cattggccaa tacaaagtgc ttgatttagt
     5761 aacgcaaggg ctacgagatt cccgactaaa catcgggtaa aagggaggat ttaaaaatgt
     5821 gattcgcata agctcaaaca aaatacatta tagtgataaa ataaactaca tttccctatt
     5881 atgaaaacga aaatttatct gttttttatc t
//
LOCUS       DAOOBB010000121         5875 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_15739_length_5875_cov_4.163574, whole genome shotgun sequence.
ACCESSION   DAOOBB010000121 DAOOBB010000000
VERSION     DAOOBB010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 5875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5875
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15739_length_5875_cov_4.163574"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..1759)
                     /locus_tag="PLT31_06375"
     CDS             complement(110..1759)
                     /locus_tag="PLT31_06375"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HPW94800.1"
                     /translation="MFHGIGFGSDLEYWRKWIESLRLGFGNFTGDYPPVFIIWLRIVS
                     WFYEVSGFSLKLEYELKLFCLIPVVLAHIALVHFIWKRLKKRTWSPEIKKVALILVVA
                     NPAFYLDGPIWGQVDLLPATICVFALWYISRPKTYALGMALFVFGLLTKFQMIMFLPV
                     VAGLSLRYRKIMWKGLFSGAFVFLLVFLPFILAGHFVDEFKQAYVSSVESYPYAAMNA
                     GNIWMMLVGNMTPDSRPLFENGPAFLNPSLLGKVFFVMVSVMVMAISFFKRNSSGKIM
                     MLGAINALAFFMLLPSMHERYCLAAAVIAVASAVLFPKPKFGTAIAFSAIAFLNISMM
                     TSLRGDSLWFWIAVGGIVAFIVALFNYTFPKKWLKACLAKIPRFQLPGITPYALLLVV
                     ISIQAFSSYLSVSRSAYDLKSNEKFVYQLDLLRQHQGYGSTQIGKSIDGNPLHINGNI
                     YIAGLGTHAPSLHEYELPDNAKSFSFTCGIDDESGNGVVNFIVELDGKRIWESGRMEG
                     RQSKSATLDISGGKRLSLITDEMGSKNFDHANWVNPIITME"
     gene            complement(1940..2836)
                     /locus_tag="PLT31_06380"
     CDS             complement(1940..2836)
                     /locus_tag="PLT31_06380"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPW94801.1"
                     /translation="MKDLIIIGAGCAGLTAALYAARAGKSVLIFEKESIGGQIASSPL
                     IENYPGVPRMSGIEFADHLFSQVTELGVELEIDEVIKIESEGSVHHVFTSSQKYDAKA
                     VIIATGAKHRTLGLEKEELFTGHGVSYCALCDGAFFKNKRVAIVGGGNTAITEAIYLS
                     TYCESVTIIHRRDSFRADLSLMNQAESKSNIFWTKDTIIQELVGEKSLSGLKLLNTKT
                     NSTSLLSVEGLFVAIGHLPQTKAFSELVPIDAEGFFDIGEDCKSPVPSIYVAGDCRKK
                     NMRQLTTAVADGANASMAVSNT"
     gene            complement(2840..5227)
                     /locus_tag="PLT31_06385"
     CDS             complement(2840..5227)
                     /locus_tag="PLT31_06385"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HPW94802.1"
                     /translation="MYQVVKRDNKTVDFNISKISDAIIKAFEALNIDFNNDIIDLLAL
                     KVTADYAPKVKDGKITVEEIQDSVESVLQRAGYEEVAKAYILYRKQREKIRYMKSAVL
                     DYKELVDSYVKVNDWRVKENSTVTYSVGGLILNNSGAITANYWLTELYDEEIAKAHRH
                     ADIHLHDLSMLTGYCAGWSLKQLIQEGLGGIPGKITSAPAKHLATLCNQMVNFLGIMQ
                     NEWAGAQAFSSFDTYLSAFVKADNLSYEQVKKCIESFIYGVNTPSRWGTQAPFSNITL
                     DWTVPNDLAELNAIVGGKEMDFKYKDCKKEMDMVNRAFIEVMIEGDANGRGFQYPIPT
                     YSITNNFDWSDTLNNQLLFEMTSKYGTPYFSNYINSDMEPSDVRSMCCRLRLDLRELR
                     KKSGGFFGSGESTGSVGVVTINLPRIAFLAVDEEDFFKRLNKMMDISARSLKIKRNII
                     TKLLDEGLYPYTKRYLGSFENHFSTIGLIGMNEVGLNAKWLKKDLTHSETQDFAKKVL
                     NHMRERLADYQEKYGDLYNLEATPAESTTYRLAKHDKKHYPEIITASKNEEVPYYTNS
                     SHLPVSFTSDIFDALDIQDQLQTLYTSGTVFHAFLGEKMPNWKAAANLVKKIAENYKL
                     PYYTLSPTYSICRDHGYLPGEQHSCPICGGVTENYSRITGYYRPVQNWNDGKSQEFKE
                     RKLYDIDNSVLKAKGASSQIVEPCQVEVKDLNGIASTLDNKILLFTSKTCPNCKMAKI
                     ILDSSDLKYEIVDAEENLDLTNQFKIQQAPTLVVVSSGNVQSFANVSNIKKYVKG"
     gene            complement(5249..>5875)
                     /locus_tag="PLT31_06390"
     CDS             complement(5249..>5875)
                     /locus_tag="PLT31_06390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HPW94803.1"
                     /translation="CNFRCPFCHNASLVLPEKMIPSSITEDSFFEFLDSRKNKLEAVC
                     ISGGEPLLQKDIAPFIKKIKELGFLIKLDTNGFIPTQLKELVNQRLIDYVAMDLKNSP
                     QKYASTAGISNFNIDKINESISFLLSKAVPYEIRSTIVDDFHSESDIEAMAQWIQGDS
                     TYYLQQFADSGDVIQSGLHSPNAEKMIHYQKIARKYVPNTQCRAINSI"
BASE COUNT         1840 a         1113 c         1121 g         1801 t
ORIGIN      
        1 tattttatag ttattcataa acagaaagca cctttgttta tgctataaat atacaccctg
       61 ccctaaggtg agagtcaaga ggttgttgtc taagaagaca attttttttc tattccattg
      121 taatgattgg attcacccag ttggcgtgat cgaaattctt agaacccatt tcatcggtga
      181 tgagagatag tcttttcccg ccactaatat ctaaagttgc cgatttagat tgtcttcctt
      241 ccatacggcc gctttcccaa attctttttc catctaattc tactataaaa ttaacgacgc
      301 cattgcctga ttcatcatca atgccgcacg taaaggaaaa tgattttgcg ttatcaggga
      361 gttcatattc atgaagtgat ggggcatgcg tgccaagccc tgctatataa atattcccat
      421 tgatgtgtag gggattacca tctatacttt ttccaatctg agtgcttcca tagccttgat
      481 gttgacgtaa taggtctaat tgatagacaa atttttcatt ggattttaag tcgtaggcgg
      541 aacgtgatac agagaggtat gatgaaaaag cttggattga aatgactact aataataagg
      601 catagggagt gattccaggc aattgaaatc taggaatttt agctaggcat gcttttaacc
      661 attttttagg gaaagtgtaa ttaaaaagtg ctacgataaa agcgactatg ccaccgacgg
      721 caatccaaaa ccaaagtgaa tcgcctcgta aagaagtcat catagaaata ttcaagaatg
      781 caattgcaga aaaagcgatg gctgttccaa attttggttt aggaaataaa acggcagaag
      841 caacggcaat cactgcagca gctaggcagt agcgttcatg catactaggg agtagcatga
      901 aaaaggcgag tgcattgatg gcaccaagca tcatgatctt gccagaagaa tttcgtttga
      961 aaaaagaaat agccataacc ataacagaga ccattacaaa aaagactttt cctaataggc
     1021 tagggtttaa aaaggctggc ccattttcaa aaagaggtcg agaatcagga gtcatgtttc
     1081 cgacaagcat catccagata ttacctgcat tcatagcggc gtaagggtaa gattcaacgc
     1141 tgctcacata ggcttgcttg aattcatcta caaaatgacc tgctaagata aagggtaaaa
     1201 agactaggag aaaaacaaac gctcctgaga ataatccttt ccacatgatt tttcgatagc
     1261 gaagggaaag ccctgcgacc actggtaaaa acataatcat ctggaatttg gtgagtaagc
     1321 caaagacaaa aagtgccatg cctaaggcat acgttttagg acgagaaata taccaaaggg
     1381 caaatacaca aatggtggct ggcaaaaggt ctacttgccc ccaaataggc ccatctaaat
     1441 agaatgcagg gttagcgact acgagaatta aggccacttt ttttatttca ggagaccaag
     1501 ttcttttttt aaggcgtttc caaataaaat ggacaagcgc gatgtgtgct aatacaacag
     1561 gtataaggca aaaaagtttt agttcgtatt ctagttttaa ggaaaagccg ctgacttcat
     1621 aaaaccaaga aacgatacga agccaaataa taaaaacagg agggtagtca ccagtaaaat
     1681 ttccaaaacc aagacgaagg gattctatcc acttacgcca atattcaagg tcagagccaa
     1741 accctattcc atgaaacatg atgaaattta aaaaaagtgt caatatgccc caaggagcaa
     1801 atgaattccc cttgaaaaat gtttcataaa aagattgttt catttgtaag aatttggctt
     1861 taatcataag ttgcttttga ccccactttg ccgacaaata taaaaaactt tatgggcttg
     1921 aatttcaaaa gcaagagtct tatgtattag aaaccgccat agaagcgttt gccccatctg
     1981 caactgccgt agtcaattgg cgcatattct tctttctaca atctccagcc acataaatac
     2041 taggcacagg ggatttgcaa tcttcaccaa tatcaaaaaa gccttcagca tctataggga
     2101 ctaactccga aaatgctttt gtttgcggta ggtgtcctat cgctacaaaa aggccttcta
     2161 cagataacag agaagttgaa ttcgttttcg tgtttaataa ttttaagcca gaaaggcttt
     2221 tttcgcccac gagttcttga ataatcgtat ctttagtcca aaagatattg cttttggatt
     2281 ctgcttgatt cattaagctt aaatcggctc taaaagaatc tcttcggtga ataatagtga
     2341 cgctttcgca gtacgtagat aagtagatgg cttcggtaat cgctgtgttt ccaccgccaa
     2401 caatcgccac gcgtttgttt ttgaagaaag ccccatcgca aagagcgcaa taagaaacac
     2461 cgtgcccagt gaatagttct tctttttcta aacctaaagt acggtgtttt gctccagtag
     2521 caataatgac ggctttcgcg tcatattttt ggctagaggt aaaaacgtga tgaacggatc
     2581 cttcggattc tattttaatg acctcatcaa tctctaattc aacaccaagt tctgtcactt
     2641 gagaaaataa atgatcggca aattcaatac cgctcatacg aggtactcca ggatagtttt
     2701 ctatcaatgg agaagaagcg atttgaccac caatactttc tttttcaaaa ataagaacag
     2761 acttacctgc gcgtgcggca tagagagcgg ctgttaaccc agcacagcca gctccgatga
     2821 tgatgaggtc cttcataggt tatcctttta cgtatttctt gatattagag acgtttgcaa
     2881 aggattgtac gtttcctgaa ctgacaacga ctagtgtagg agcttgttgt atcttgaatt
     2941 gattagtcaa atcaagattt tcttcagcat ctacaatttc gtatttcaaa tcactggagt
     3001 ctaaaattat ttttgccatt ttacaattag gacaagtctt gcttgtaaac aagagtatct
     3061 tattgtctaa tgtggaagca attccattta ggtctttcac ttctacttgg caaggctcta
     3121 caatttgaga agaggctcct tttgctttaa gaaccgaatt atcgatatca taaagttttc
     3181 tttccttaaa ctcttgagac ttaccatcat tccagttttg aacaggtctg tagtatccag
     3241 taatacggct gtaattttct gtaacaccac cgcagatagg gcaagagtgt tgctctcctg
     3301 gaagatagcc atgatctcta cagatagaat aagttggaga taaggtgtag taaggaagtt
     3361 tatagttttc agcaatcttt tttaccaaat tcgctgccgc tttccagtta ggcatttttt
     3421 ctccaaggaa cgcgtggaaa acggttcctg aagtataaag cgtttgaagt tgatcttgaa
     3481 tatcaagggc atcaaaaata tcagaagtaa agcttactgg taaatgagaa ctattggtat
     3541 agtaaggaac ttcttcattc ttagaagccg tgatgatttc agggtaatgc tttttatcat
     3601 gcttggctag gcgataggta gtagactctg caggagtcgc ttccaagttg taaagatcgc
     3661 catatttttc ttgataatca gcaagacgtt ctctcatatg attcaacact tttttagcaa
     3721 aatcttgagt ttcgctatga gtcaaatctt ttttaagcca cttggcattt aatcccactt
     3781 cgttcatgcc tattaaacca atagtagaga agtgattctc gaacgaacca agatagcgtt
     3841 tagtataagg ataaagccct tcatccaaaa gttttgtgat gatgtttctc ttgattttta
     3901 aggaacgagc agaaatatcc atcatcttat tgaggcgctt aaagaagtct tcttcgtcta
     3961 cggcaaggaa agcaattctt ggtaagttaa tcgttacaac accaacagaa cctgtgcttt
     4021 caccgcttcc aaagaagcca cccgattttt tacgaagctc tcttaagtca agtcttaagc
     4081 gacagcacat actacgcaca tcgctaggtt ccatatcgct attgatatag ttagagaaat
     4141 aaggagtccc atatttagag gtcatttcaa ataacaattg gttatttaaa gtatctgacc
     4201 agtcaaaatt attggtgata gaataagttg ggataggata ttggaatcca cggccattgg
     4261 cgtctccttc aatcatcact tcaataaaag ctctgttgac catatccatt tcttttttac
     4321 aatctttata tttgaaatcc atttccttgc caccgacaat cgcatttaat tcagcaaggt
     4381 catttggcac ggtccaatct agagtgatat tagagaacgg agcttgtgtt ccccaacggc
     4441 taggtgtatt cacaccataa ataaaggatt caatgcattt cttcacttgt tcataagaaa
     4501 gattgtctgc ttttacaaat gcagataaat aagtatcaaa agaagaaaat gcttgagcgc
     4561 cagcccattc attttgcata atacctaaga agttcaccat ttggttacaa agggtggcaa
     4621 ggtgttttgc tggagcagaa gtgatttttc caggaatacc accaagacct tcttggatca
     4681 attgttttag agaccaacca gcgcaatagc ctgttagcat cgaaagatca tgaagatgaa
     4741 tgtctgcatg gcggtgtgcc ttagcaattt cttcatcata aagttctgta agccagtagt
     4801 ttgcagtaat ggcacctgag ttatttagaa tcaaaccacc cacagaataa gtcacggtgg
     4861 aattttcttt aacacgccag tcgttgactt tcacataact atcgactagt tctttataat
     4921 caagaacagc agatttcata tagcggattt tttcgcgttg cttacgatat aaaatgtaag
     4981 ccttggccac ttcttcgtaa ccagcacgtt gtaatacact ttccacacta tcttgaattt
     5041 cttcaacagt aattttgccg tcttttactt taggggcata gtcggcagtg acttttagcg
     5101 caagcaagtc aatgatgtcg ttattaaaat caatgtttaa agcttcgaaa gctttaatga
     5161 tggcgtcgct gatttttgaa atgttaaaat caactgtttt gttgtctctt tttacaactt
     5221 gatacatatt gtgttctcca aataaatctt aaatactgtt tattgctcga cattgggtgt
     5281 taggaacata ttttcgagct attttctgat aatgaatcat tttttcagcg tttggggaat
     5341 gaagaccgct ttgaattacg tctcctgaat ccgcgaattg ctgaagataa taagtgctgt
     5401 caccttgaat ccattgagcc atggcttcta tatcactttc agaatgaaag tcatctacaa
     5461 tcgtggatct aatttcgtaa gggacagctt ttgagagtaa gaaagaaata ctctcattta
     5521 ttttatcgat attaaaattc gatattcctg cggtggaagc gtatttttgt ggactattct
     5581 tgagatccat cgcaacatag tcaattaatc tttgattgac taactctttt aattgagtcg
     5641 gaataaatcc attagtatcc aattttatta aaaagcctag ttcctttatt ttcttaataa
     5701 agggagcaat atctttttgt agtaaaggtt ctccaccaga aatacaaact gcttccagtt
     5761 tattttttct agaatctaaa aactcaaaaa aagaatcttc agttattgaa cttgggatca
     5821 ttttttcggg gagtactaat gaggcgttat ggcaaaaggg acaacgaaaa ttaca
//
LOCUS       DAOOBB010000122         5872 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_15747_length_5872_cov_4.653602, whole genome shotgun sequence.
ACCESSION   DAOOBB010000122 DAOOBB010000000
VERSION     DAOOBB010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 5872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5872
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15747_length_5872_cov_4.653602"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PLT31_06395"
     CDS             complement(<1..469)
                     /locus_tag="PLT31_06395"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HPW94804.1"
                     /translation="MKIYPVQMNIVPGKPELNFETLKKEVEKAVLNQCDLVVFPEMCL
                     SGYLIGDLWEEHSFIDQCVEYGNRVANLSASIDILFGNVAREEIHGENGRPLLYNAAF
                     YASNGYFEPLVQCNGVIKNFLPKTLLPCYREFDEPRYFFDLRRLAYSLNQPLEA"
     gene            complement(617..2434)
                     /locus_tag="PLT31_06400"
     CDS             complement(617..2434)
                     /locus_tag="PLT31_06400"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HPW94805.1"
                     /translation="MSLTKHAKLNAWIEEIQAMVKPENVVLCDGSQAQYDNLIKEMVE
                     SGLATKLNQEKLPGCYLFRSDASDVARVEDRTYISSEKQEDAGPTNNWKAPAELKAEM
                     KALYDGCMKGRTMYVIPFSMGPVGSDIAKIGVEITDSAYVVINMMIMTRVGSKVLDVL
                     GTDGEFIPCLHSVGSPLQPGESDNGKWPCAPIEKKYISHFPEERTIWSYGSGYGGNAL
                     LGKKCLALRIASAVAHEEGWLAEHMLILKITSPEGVVKYVCGAFPSACGKTNLAMLIP
                     TIPGWKVETIGDDIAWMKPGKDGRLYAINPEAGFFGVAPGTNFHSNKNAMLSAQKDTI
                     YTNVALEADGTVWWEGMDEDPKSELIDWKGNKWNLPANGKSEKGKEAAHPNARFTAPA
                     RNCPAIAPEWEDPKGVPVDAFLFGGRRPSTIPLVHQAMSWEHGVFMGSIVGSEVTAAV
                     ISDQVGQVRRDPFAMLPFCGYHMGDYFAHWIKMGKANPDKMPKIFFVNWFRKTKEGKW
                     LWPGYGDNSRVLKWVCERCDGKGDAVETPIGFMPTATAIDRPEGVTVEDMNELLSVDK
                     AGWLAELADVEKNHYPKFGDKLPQELRDQIKIITERLSK"
     gene            2640..3890
                     /gene="glgC"
                     /locus_tag="PLT31_06405"
     CDS             2640..3890
                     /gene="glgC"
                     /locus_tag="PLT31_06405"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014544985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HPW94806.1"
                     /translation="MSWSYREHQKNILCMIMAGGQGSRLQPLTRDRAKPAVHFGGTYR
                     IIDFVLNNFINSGIFKIKVLTQFKSDSLNKHISAAWSLNSSLDQYVDLVPAQMRTGED
                     WYRGTSDAIFQNINLITDERPDLVAIFGGDHIYKMDINQMIDFHLSRAALLTIAAIPV
                     PVSEASEFGIIEVDSDNRMIGFKEKPKEPKEMPGNPGWCLASMGNYIFTSKFLVRELL
                     NGSKQGATDFGKHIIPALYKEYPVYVYDFNTNIVRGEQASTKGYWRDVGTLDAFFEAN
                     MDLCAENPLFDLYNNYWPIRTFNWNQPPARFFGNDDSTTYRGAAIDSIVSAGSIIGGG
                     TVVKSILSPGTSIQKEAFVKESILFPNVTIGPGAKVQRAIIEKGLHIPAGFEIGFDLE
                     RDRQIFHVTDSGIVVLAKDTIIKA"
     gene            complement(3893..5008)
                     /locus_tag="PLT31_06410"
     CDS             complement(3893..5008)
                     /locus_tag="PLT31_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HPW94807.1"
                     /translation="MLKVDNILDPSSIKRSFSLGSLSVFPNTVLSPMAGVTDPPFRRL
                     CRELSKNRIGLLVSEFVSTDGGSEFSLKNRKELKFYPEERPFGIQIFGRDPERMARAA
                     KLIETLKPDFIEVNAGCPVPKVAGKGGGAGLLRDLNRLALILRKVKSSIQVPMTLKCR
                     IGWDETQINVFETLQIAEDEGAELLTVHGRTRVQGYNGFANWDIIGETASRAHIPVVG
                     NGDVVSVQTAFEKLQQYPIAGVSIGRGALHNPWLFGQIAEAFDGKPPFEISIREALSI
                     FEKYFGYMMEEDISSLGALGRLKQLAARLCKGLNPENPLFRQSLLTSETPEMFFERIA
                     LFLEDKESLNTSFYPDRLVNLNGKKENEIVFGRQFKG"
     gene            5113..5460
                     /locus_tag="PLT31_06415"
     CDS             5113..5460
                     /locus_tag="PLT31_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPW94808.1"
                     /translation="MPNVTKQDLIQEIAKSTGFIRNDIKMVIEQFLSIVGEKLIENKT
                     IELRGFGTFACKERKARPARNPRTGEPVLLEERLVPTFKFSSDIKDKINFTDTLQAEK
                     VLTEEKENSNSIH"
     gene            complement(5443..>5872)
                     /locus_tag="PLT31_06420"
     CDS             complement(5443..>5872)
                     /locus_tag="PLT31_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPW94809.1"
                     /translation="MKIRSVGRLDVDSSGLLLLSNKGDFIHHLESPKRGVLKRYDVTL
                     ARPLEKTQEEQLLSGVILKNEKRAVFARSLEKKSETQVEIEIGEGLYHQVRRMFAAVG
                     NHVEELRRLSIGGLNLDQQLKPKEWKYLTESEIDQLMNRI"
BASE COUNT         1852 a         1282 c         1059 g         1679 t
ORIGIN      
        1 aagcctcaag cggttgattt aacgaataag caaggcgacg tagatcgaaa aaataacgag
       61 gctcgtcaaa ctctcgataa caaggcaaaa gcgtcttcgg caaaaaattc ttgatcactc
      121 cattgcactg cactaaaggt tcaaaatatc cattggaggc ataaaaagcg gcattatata
      181 aaagaggacg cccgttttca ccatgaatct cttcacgagc cacattacca aacaatatat
      241 cgatagacgc agataaattc gctacgcgat ttccatattc cacacattga tctataaaag
      301 aatgttcttc ccaaagatca ccaatcaaat atcccgaaag gcacatttct ggaaacacaa
      361 ctaaatcaca ttgatttaaa accgcttttt ccacttcttt tttcaaagtt tcaaaattca
      421 gttctggctt tccagggaca atattcattt gaacaggata aatcttcata ttcaaaattt
      481 agctttctac cctctcaaaa aaaatgattt aaaagaaaaa agtacttcaa agatcaattg
      541 aaaaagccta tacaacgttt aaaataaaaa agcaccccaa gtgaatcact tggagcgcct
      601 ttaatttcag gtcaaattac tttgacaaac gttctgtaat gattttgatt tggtctctaa
      661 gttcttgagg gagcttatca ccaaacttag gataatgatt cttttcaacg tcagcaagtt
      721 cagctaacca gccagcctta tcaacggaga gaagttcatt catatcttct acagtaacgc
      781 cttctggacg atcaatagct gttgcagttg gcatgaaacc aattggggtt tccactgcat
      841 cacccttgcc atcgcaacgt tcgcaaaccc atttaagaac gcgagagtta tcaccataac
      901 caggccataa ccacttgcct tccttagtct tacggaacca gttcacaaag aagatttttg
      961 gcatcttgtc tggattagct ttacccatct taatccaatg agcaaagtag tcacccatgt
     1021 ggtaaccgca gaatggaagc attgcaaatg ggtcacgacg aacttgacca acttggtcag
     1081 agatcacagc agcagtgact tcagaaccga cgatagaacc catgaataca ccatgttccc
     1141 agctcatagc ttgatgaact aaaggaatgg tgcttggacg acggccaccg aagaggaatg
     1201 catcgactgg aacaccctta ggatcttccc attcaggagc gatcgctggg cagtttcttg
     1261 ctggtgcagt aaagcgagca tttggatgag ctgcttcttt acccttttca gatttaccgt
     1321 tagcaggaag attccacttg ttacccttcc agtcaattaa ttctgactta ggatcttcgt
     1381 ccatgccttc ccaccaaaca gtaccatcag cttcgagagc tacgttagtg tagatggtat
     1441 ctttttgagc agaaagcata gcattcttat tgctatggaa gtttgtacct ggagccacgc
     1501 caaagaagcc agcttctggg ttaatagcat agagacgacc atctttacct ggtttcatcc
     1561 aagcgatatc atcaccaatg gtttctactt tccaacctgg gattgtagga atcaacatag
     1621 cgaggtttgt cttaccacaa gcgctaggga aagcaccgca aacatacttc acaacgcctt
     1681 caggagatgt gatctttaag ataagcatat gttcagcaag ccagccttct tcatgagcta
     1741 cagcacttgc aatacgtaga gctaagcact tcttaccgag aagagcattt ccaccgtaac
     1801 cagaaccata agaccaaatg gtacgttctt ctgggaagtg agaaatgtat ttcttttcaa
     1861 ttggagcaca tggccatttg ccattatcgc tttcgcctgg ttgtaaagga ctacccacag
     1921 agtgaaggca aggaataaat tcgccatcgg taccgagaac atctaaaacc ttggagccaa
     1981 cgcgagtcat aatcatcatg ttgatcacca cgtatgcaga atcagtgatt tccacaccaa
     2041 tttttgcaat atcagaacca acagggccca tggagaaagg aatcacatac atagtacggc
     2101 ctttcatgca accatcataa agagccttca tttctgcttt taattcagca ggagctttcc
     2161 agttgtttgt tggaccagca tcttcctgtt tttcggaaga gatatatgta cgatcttcaa
     2221 cgcgagcgac gtcagaagcg tcagagcgga ataaatagca acctggtaat ttttcttgat
     2281 ttaatttggt agcgagaccc gattctacca tttctttaat aagattatcg tattgagctt
     2341 gagatccgtc gcaaagaacg acattttctg gcttgaccat tgcctgaatt tcttcaatcc
     2401 aagcattcaa tttggcatgt ttagtcaggg acatttcatc tcctattaga tgggttaata
     2461 catagtttcg atttatttcg aacgcctata aactagaaat tttaaaccat aaaaaaagag
     2521 taaaacaaca tagaaaatca aaaaaaagag cttacttaaa aaaacttacc ccaagccctt
     2581 ataaataaga taaattaaga caaaaacgct attagaattg atcaaaaaac agaggaaaca
     2641 tgagctggtc ttatagagaa catcaaaaga atattctatg catgattatg gcaggtggac
     2701 aaggaagtcg tcttcaaccc ctaactcgag atcgagctaa acccgcagtc cattttggtg
     2761 gtacttatcg cattattgat ttcgttttaa ataatttcat caacagtggt atttttaaaa
     2821 ttaaagtttt aactcaattt aagtcagact ctcttaacaa gcatatttca gccgcttgga
     2881 gcctcaattc aagcttagat caatacgtgg accttgttcc cgctcaaatg cgtacaggtg
     2941 aagattggta ccgcggtacc tccgatgcga ttttccaaaa catcaattta attaccgatg
     3001 aacgccctga tctagtcgct atttttggcg gggatcatat ctataaaatg gacatcaatc
     3061 agatgattga ttttcatttg agtcgcgccg cattacttac tattgctgca atccctgttc
     3121 ctgtcagtga agcaagcgaa tttggaatta tcgaagtcga ttcagataat cgaatgattg
     3181 gctttaaaga aaaacccaaa gagccaaagg aaatgccagg aaaccctggt tggtgccttg
     3241 ccagtatggg taactacatt tttaccagta aatttttagt acgtgaactt cttaatggct
     3301 caaaacaagg ggccactgac tttggtaagc acattattcc tgctttatac aaagaatatc
     3361 ctgtttacgt ttacgatttc aacaccaata ttgttcgcgg agaacaagcc tctacaaagg
     3421 gctattggcg agatgtaggt acattagacg ccttctttga agcgaatatg gatctttgtg
     3481 ccgaaaaccc cttatttgac ctttataata actactggcc tatcagaact tttaactgga
     3541 atcagccacc agctcgtttc tttggtaatg atgattccac tacttatcgt ggagcagcca
     3601 tcgattcgat tgtttctgca ggcagtatca ttggtggagg cacagtagtc aaaagtattc
     3661 taagcccagg aacttccata caaaaagaag ccttcgtgaa agaatctatt ttgttcccaa
     3721 atgtcactat cgggcctgga gccaaagtgc agagagccat tattgaaaaa ggcctccata
     3781 ttccagcggg ttttgaaatc ggatttgatt tagagcgaga taggcaaatt ttccatgtaa
     3841 ccgattctgg catcgtggta cttgccaaag acacgattat caaagcttaa acttatccct
     3901 taaactggcg accaaataca atttcatttt cttttttccc gttcaaattc accaatcgat
     3961 cgggataaaa agaagtattt aaggattctt tgtcttcaag gaatagagcg attctttcaa
     4021 agaacatctc aggggtttca ctagtcaata gagattgtct aaagagcgga ttctctggat
     4081 taagaccctt acaaagcctt gctgctaact gcttaagacg gcctaaagcc cctaatgaag
     4141 aaatatcttc ttccatcata tacccaaaat atttttcaaa aatcgaaagt gcctctcgga
     4201 tcgatatttc aaaaggaggc ttgccatcaa aggcctctgc aatttgccca aaaagccatg
     4261 ggttatgaag agcacctctg ccaatactca ccccagcaat cgggtattgt tgcaactttt
     4321 caaaggccgt ttgaacgcta accacatctc cattaccaac aacaggaata tgcgctctag
     4381 aagccgtttc tccaatgata tcccagttag caaagccatt atagccttga actcgcgttc
     4441 tcccgtgaac cgtcaacagt tcagcgcctt catcttctgc tatttgaaga gtttcaaaaa
     4501 cattaatctg tgtttcatcc cagccaattc gacatttaag agtcattggc acttgtatac
     4561 ttgatttcac ttttcttaaa atcaaagcca agcgattcaa atctcgtaaa agcccagctc
     4621 caccgccttt cccagccact tttggtacag gacaacctgc atttacttca ataaaatcag
     4681 gttttagtgt ttctattaat ttagctgctc gagccatccg ttctggatct cggccgaaaa
     4741 tttgaattcc aaaaggacgt tcttctggat aaaactttaa ttcttttctg ttttttaagg
     4801 aaaattcaga tccaccgtct gtagagacaa attcagaaac taaaagtccg attctatttt
     4861 tagacaattc acgacaaagc cttctaaaag gaggatccgt aacgcctgcc atcggactta
     4921 gaacagtatt tgggaaaacg gataaagaac ctaaagagaa agatcgcttt atggatgatg
     4981 gatcaagaat attatcaact ttcaacattt tactcacaac aattcacctt atatcctcag
     5041 cttttaatca aaaaaaatat cttattttgg tcgagtccta ttttttttta ttgataaacc
     5101 tatattttga aagtgcctaa tgtaacaaaa caagatctaa tccaggaaat cgccaaatca
     5161 acgggcttta ttcgaaacga cattaaaatg gtcatcgaac aatttttaag cattgttggc
     5221 gaaaaactca tcgaaaataa aacaattgag cttcgtggtt ttggaacatt tgcttgtaaa
     5281 gaacgtaaag ctaggccagc acgaaatcca agaacaggtg aacctgtttt attggaagaa
     5341 cgcctagttc caacgttcaa attttcttct gacattaaag ataagatcaa cttcactgat
     5401 actcttcaag ctgaaaaagt tcttactgaa gaaaaagaaa actcaaattc tattcattaa
     5461 ttgatcaatt tcagactcag tcaagtattt ccattccttg ggctttaact gctggtctaa
     5521 attcaagcca ccaatggata atcgacgtag ttcttcgaca tgattcccta ctgccgcaaa
     5581 catacgacgc acctggtgat aaagaccttc tccaatttca atttcaactt gagtctcaga
     5641 ctttttttca agtgagcgag caaacactgc acgcttttca tttttgagaa tcactccaga
     5701 gagtaattgt tcttcttgag tcttttcaag aggccttgct agagtcacgt cataacgctt
     5761 tagaacgccc cttttagggc tttctaaatg atgaataaag tcacccttat tcgaaagaag
     5821 taaaaggcca gaagaatcca catctaaacg gccaacagat ctaattttca tt
//
LOCUS       DAOOBB010000123         5863 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_15774_length_5863_cov_5.343664, whole genome shotgun sequence.
ACCESSION   DAOOBB010000123 DAOOBB010000000
VERSION     DAOOBB010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15774_length_5863_cov_5.343664"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="PLT31_06425"
     CDS             complement(<1..801)
                     /locus_tag="PLT31_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94810.1"
                     /translation="MNLKLLVGIGMLAATSIWAQGTFDGDMSSPSEESSSFDAPAPDP
                     VPAPIYQPAQEESSPASSSSEVAGGDESLNDFNKLHGNAYNTVGNEGAFATVGTNMRA
                     PHTMKGQNLVYFEPSLDYGLVSFTKGSTFFLALDNSGKVGLMTLGMAMSGMGFSLDLG
                     LGKTFISSETAGVESSGSTIAFGDDIGFRFSMPLGGLSFAAHLEWLTIVDQVSAETET
                     PAAETNANYWDLLLEAEISNAPSAKDFFWTGGANILRRNLTEENIGATT"
     gene            1020..2051
                     /locus_tag="PLT31_06430"
     CDS             1020..2051
                     /locus_tag="PLT31_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94811.1"
                     /translation="MKFFYSFWLILGLSFPVLNWASNIISPSLDSQSIQTRDFYKHPE
                     TSKDFNETWSYSLFFSNGTKAYINFASLPIPSQGRKIGCDLSFWNPKWKNPSVGRQYP
                     PERFIEQKEKNRITIKEEYFMENLPGKGHRVFFSADKGGRFFVDITFTSAIPGIVPGK
                     GIWKIGSAQFAQFIHIPYGKVSGRIGFNEDTIAVSGYGYMDHVYQSVSTIDFVNRSLS
                     FSMPYSNEMFAGRLSISKEGTTLGYGVYIQNGKPEILIPKQILEKDKNYSGKTFPKNE
                     LKITWASSAPDLILNTSKIQQKFSILNNFDGWFAKKAAKLLMGGEVWFYRGRSYQNKN
                     AIDWSITGL"
     gene            2061..2828
                     /gene="hisA"
                     /locus_tag="PLT31_06435"
     CDS             2061..2828
                     /gene="hisA"
                     /locus_tag="PLT31_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformimino-5-aminoimidazole
                     carboxamide ribotide isomerase"
                     /protein_id="HPW94812.1"
                     /translation="MTKFRPCIDIHDGKVKQIVGSSLADSGKGLETNFESDQSPAWFA
                     SLYKKDNLKGGHVIMLGQGNEKAAKEALAAYPGGLQIGGGISTSNALEYLDAGASHVI
                     VTSWIFQNNELSWNRIAELSHLVGIHRLVIDLSCKRNYGKWNVATNRWQTITSTEITR
                     ETLNELSLYCNEFLVHAADVEGKQQGMDEELISFLGENSPIPVTYAGGARNISDLHLC
                     DSLSNGRVDLTIGSALDIFGGKGAKYFDCIQYNKEQK"
     gene            2794..4221
                     /locus_tag="PLT31_06440"
     CDS             2794..4221
                     /locus_tag="PLT31_06440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPW94813.1"
                     /translation="MTASNTTKNKNDLTHLTDSRAPIFGREVTSVFKRYFSLPHSKAS
                     NVKSLVFPVLLTGVILKNLPYLLSMRWYLKKRRKNRSSNEMRVLYFSDNLDEVNGIAN
                     NLRHVVSYMKSNGYQANLAGCAFNTRTNGVIENHYVVLTPRLYSMDQLGYANSELALP
                     NFKPVFRLFKRYPADLIELETPSPGSWAVAIGAKIMGIKVISHYRTDVPTYTCTLVKA
                     KWMHVLVLFLMRIFYNMTRPVISPCQEYSRILKKDIWVPSKAIQILPRGIPLNQFSPL
                     MRGLGVWEKFGGGRPVRFLFVGRISKEKDLPFLEKLWHLLHQETDSAELMFVGNGWYL
                     DTLKSSFSDCPEVLFAGEQGGETLASLYADADFFIFPSGTDTFGNVVVEALASGTPAI
                     VTDKGGPQDIIKDQGCGFILPFGKETIWLEKLKECIDIKLKQPEKYESMRRCAFERSR
                     FFSLENTAKSQWAFFKTLYDKSYKK"
     gene            4221..5273
                     /locus_tag="PLT31_06445"
     CDS             4221..5273
                     /locus_tag="PLT31_06445"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HPW94814.1"
                     /translation="MSSKNQLISWFLKNRKALPWRPEDPYAFRNDYFVWISEIMLQQT
                     QVAAVCEHFSRWILRFPKISDLAKASEKDVLSHWQGLGYYSRAKNILRTAQILVRDYN
                     GFLPASRKELEALPGIGKYTAGAILSLARHEKEAILDGNLIRIFSRLEKIPFLPDQKS
                     SSDFYWNIAREWSSSKKAFLINEALMELGRNCCKVKNPTCNVCPLKANCKAFISKQIS
                     LFPPKKKKSFEEWIGLVLVIESENGKIFFTDNDQSHFLKKQKTLPHFEFPYSESKNFP
                     AKAEEWISFNLVKEFHFCGRFKHSITRYKIDCNVLHVLTTQKISSKNPNWISKKNLLK
                     ETANSFSLKALKVASL"
     gene            complement(5270..>5863)
                     /locus_tag="PLT31_06450"
     CDS             complement(5270..>5863)
                     /locus_tag="PLT31_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94815.1"
                     /translation="DGLLQLYQVKIEFNQEWKDLQLGIRSSGFIEKGAETFDTTGFAE
                     KKSDDWFRYGDVSRINSWIRGWAGEIEFSYKYNDLFKASALVGMEHLWGDEERFEVEP
                     SEHWLEFNADWTINKTLLVSHSLHYRSDAQWNLRHPEPFEVPGDWFWNASFSQLFPKY
                     GLTLSGTLLHVLSDTTLEVPNGNENRTRFFCAIKKMF"
BASE COUNT         1825 a         1094 c         1059 g         1885 t
ORIGIN      
        1 agtcgttgct ccgatatttt cttcagtgag gttacgacgt aaaatgttag caccacctgt
       61 ccagaaaaaa tcctttgcag atggagcatt gctgatttct gcttctaaca ataaatccca
      121 gtaattggca ttcgtttctg ctgctggagt ttctgtttct gcagaaactt ggtctacaat
      181 tgttagccat tctaaatgag ctgcgaagga caatcctcca agtggcatag aaaagcgaaa
      241 accaatgtcg tcaccgaaag caatcgttga accagaggat tctacgccag ctgtttcaga
      301 agaaataaat gttttgccaa gacctaaatc aagactgaaa cccataccac tcatggccat
      361 accaagagtc attaagccaa ctttaccaga attatctaaa gctaaaaaga aagtactccc
      421 tttagtaaaa gaaaccaatc cataatctaa agaaggttca aaataaacta aattttgtcc
      481 tttcatggta tgtggagctc tcatgtttgt tccaacagtt gcaaaagcac cttcgtttcc
      541 aacggtgtta tatgcattac catgtaattt attaaaatca tttagtgatt catcgccacc
      601 agcaacttct gaagaagagc ttgctggaga ggattcttct tgagcaggtt gataaatagg
      661 tgcaggcact gggtctggtg caggcgcatc aaaagaactg ctttcttcag agggactact
      721 catgtcccca tcaaaagtac cctgagccca aatactggtt gctgcaagca taccaatacc
      781 aactaataat ttaagattca tagagatctc cttaaaaaaa aattccattc cgagtaataa
      841 atttataaca ttttcaacag cttttttact aaaaattatt ttttctgtaa aaaaaactct
      901 attttatacg ttttcttgca ttttttgatt aaaaacctac tgtaaaacaa actaaacacg
      961 ttttttgagg tttcattaat tttgaattat cctttcttat tttattctat cttattacta
     1021 tgaagttttt ttattctttt tggcttattt tggggttatc atttcctgtt ttaaactggg
     1081 cctctaatat tatttcgcct tcccttgact ctcaatcgat ccaaacgaga gatttttata
     1141 aacatcctga aacatcaaaa gactttaatg aaacttggtc ttattctctt ttttttagta
     1201 atgggactaa agcttacatt aattttgctt ctttacctat tccttctcaa ggaagaaaaa
     1261 tcggctgcga tctttccttt tggaatccaa aatggaaaaa tcctagcgta gggcgtcaat
     1321 accccccaga acgttttatt gaacaaaaag aaaaaaatcg cattactatt aaagaagaat
     1381 actttatgga aaatctacca ggcaaagggc atcgtgtatt tttttctgca gataaaggtg
     1441 gccgtttctt tgtcgatatt acttttactt ctgcgattcc aggtattgtt cctggtaaag
     1501 gcatctggaa aatcggtagt gcacaatttg cccaattcat tcacattcct tatggtaaag
     1561 taagtggccg aattggtttt aatgaagata ctattgctgt atctggctat ggttatatgg
     1621 atcatgttta tcaatccgtt tctactattg atttcgtgaa tcgttctcta tctttcagta
     1681 tgccttattc taacgaaatg tttgcaggtc gtttaagtat ttctaaagaa ggtactactc
     1741 ttggctatgg tgtttatatt caaaacggaa agcctgagat tcttattccg aaacaaattt
     1801 tagagaagga taaaaattac tcagggaaaa ctttccccaa aaacgagtta aaaataacat
     1861 gggcatcttc tgcccctgat ttaattctaa atacatcgaa aatacaacaa aaattttcaa
     1921 ttcttaacaa ttttgatggc tggttcgcta aaaaagctgc taaattattg atgggaggcg
     1981 aagtttggtt ttatcgaggg cgttcttatc aaaataagaa tgccattgat tggagtatta
     2041 ctggtttata aggatttttt atgaccaaat tcaggccttg tattgatatc catgatggaa
     2101 aagtaaagca aatcgttggc tcttctcttg ccgattctgg gaagggttta gaaacgaatt
     2161 ttgaatcaga ccagtctcca gcatggtttg cctctcttta taaaaaagac aacctaaaag
     2221 gtggtcatgt gattatgctt gggcaaggca atgaaaaagc agccaaagaa gctttagcag
     2281 cctaccctgg tggcttgcaa attggtggag gaatatccac ttctaatgct cttgagtatt
     2341 tagatgctgg agcttctcat gtgattgtga ctagctggat ttttcaaaac aatgaactca
     2401 gttggaatcg aatcgccgaa ttatctcacc ttgttggcat ccatcgttta gtcattgatt
     2461 taagttgcaa aagaaactat ggaaaatgga atgtggccac gaatcgctgg caaaccatca
     2521 cttctacaga aatcactcgt gaaacactta atgagctttc cctttattgt aacgaatttt
     2581 tagttcatgc tgctgatgtc gaagggaaac aacaaggaat ggatgaagaa ctgatttctt
     2641 tcttaggaga gaactcccct attcctgtaa cctatgctgg tggggcacgt aatattagtg
     2701 acttacattt atgcgattcc ttatctaatg gaagagtgga tcttaccatt ggcagtgctt
     2761 tagatatttt tggtggaaaa ggagcgaagt attttgactg catccaatac aacaaagaac
     2821 aaaaatgatt taactcatct cactgactca agagcaccta tttttgggcg cgaagttact
     2881 agtgttttta aacgttattt ttcactacct cattctaaag caagtaacgt aaagtctctt
     2941 gtattccctg ttttattgac aggtgttatt ctaaaaaact taccttatct gctttcaatg
     3001 cgatggtatc taaaaaaaag acgcaaaaac cgatcctcca atgaaatgcg agttctctac
     3061 ttttccgata atttagatga ggtcaatggg attgccaata atttaagaca tgttgttagt
     3121 tatatgaaaa gtaatggcta tcaagctaat ttagctggct gtgcttttaa tactagaact
     3181 aatggagtga ttgaaaatca ctatgtagtg cttactccac gcctatactc tatggatcaa
     3241 ctcggctatg ctaatagtga actagctctt cctaatttca aacccgtttt tcgtttattt
     3301 aagcggtatc cagccgattt aattgagctt gaaactccaa gccctggctc ttgggctgta
     3361 gctattggcg ctaaaataat gggaatcaaa gtgattagtc attatagaac agatgtacct
     3421 acctatactt gcactttagt caaagcaaag tggatgcatg tactagttct atttttaatg
     3481 cgcatttttt ataacatgac tcgccctgtg atatctcctt gtcaagaata tagtcgtatt
     3541 ctaaaaaaag atatttgggt gccttcaaaa gcgattcaaa tacttcctcg agggatccct
     3601 ctgaaccaat ttagtccttt gatgcgtggc ttaggagtat gggaaaaatt tggaggaggc
     3661 cgtcctgtac gctttctttt tgtaggtaga atttctaaag aaaaggactt accattttta
     3721 gaaaaacttt ggcacttatt gcaccaagaa acagattctg cagaactcat gtttgttgga
     3781 aacggctggt atttagatac actcaaatcc agtttttcag attgtccaga agttcttttt
     3841 gctggtgaac aaggtgggga aacgttagca agtctttatg cagatgccga cttctttatc
     3901 ttcccaagcg gtacagatac gtttggtaac gtggtggtag aagctctagc ttcaggcacg
     3961 ccagcgattg tgactgataa aggcgggccc caagatatta ttaaagacca gggctgtggc
     4021 tttattcttc cttttggtaa agaaactatc tggcttgaaa agttaaaaga atgtattgat
     4081 attaaactaa aacaaccaga aaaatatgag tcgatgagac gctgtgcttt tgaacgcagc
     4141 cgtttcttct ctttagaaaa tacggctaaa agccaatggg cattttttaa gactctttac
     4201 gataagagct ataaaaaata atgtcttcaa aaaaccaact tatttcttgg tttctaaaaa
     4261 acagaaaggc actcccttgg cgccctgaag atccttacgc ttttcgaaat gattattttg
     4321 tttggattag cgaaatcatg ttacagcaaa ctcaagtggc cgctgtttgc gagcactttt
     4381 ctcgttggat cttaagattt cctaaaatta gcgatttagc aaaagcctct gaaaaggacg
     4441 ttttatccca ttggcaaggg cttggatatt attctagagc aaaaaatatt ttaaggactg
     4501 ctcaaattct agttcgtgat tacaatggtt ttttacctgc ctcaagaaaa gaattagaag
     4561 ctcttcctgg gattggaaaa tataccgctg gagccatttt aagccttgct aggcatgaaa
     4621 aagaagccat tcttgacggg aacttaattc gtattttttc gagattagaa aagattcctt
     4681 ttttaccaga tcaaaaatca tcttccgatt tttattggaa tatagctcga gaatggagct
     4741 cttctaaaaa agcgtttctt attaacgaag cgttaatgga actcggtcgt aattgttgta
     4801 aagtaaagaa tcccacttgt aatgtttgcc cattaaaggc aaattgtaaa gcattcatat
     4861 caaaacaaat ctctttgttt ccgcccaaaa aaaagaaatc ttttgaagaa tggatagggc
     4921 ttgtcttagt cattgaaagc gaaaacggaa aaatcttttt taccgataac gatcaatctc
     4981 attttctaaa aaaacaaaag acactccccc attttgaatt tccatattca gagtctaaaa
     5041 atttccctgc aaaagcagaa gaatggattt cttttaacct tgtgaaagaa tttcattttt
     5101 gtggaaggtt caagcattct attacgcgat ataaaatcga ttgtaacgtt ttgcatgttt
     5161 taacgactca aaagatttct tctaaaaacc caaattggat ttctaaaaaa aatcttttga
     5221 aagagaccgc caattctttt agcttaaaag ctctaaaagt ggcttcactc taaaacattt
     5281 ttttgatcgc acaaaaaaat cgagttctat tttcattccc attaggtact tctaacgtgg
     5341 tatctgataa aacatgtaac aatgttcccg agagcgttaa cccatatttt gggaataatt
     5401 gtgaaaagct cgcattccaa aaccaatcgc caggaacttc aaaaggttct gggtggcgaa
     5461 ggttccactg tgcatcactt cgataatgta aggaatgaga aaccaataat gttttattaa
     5521 tagtccaatc ggcattgaat tcaagccagt gttcggaagg ttctacctca aagcgttctt
     5581 catcacccca aagatgttcc atccctacaa gagcacttgc cttaaaaagg tcattatatt
     5641 tataagaaaa ctctatttct cctgcccagc cgcgaatcca agagttaatt cttgaaacat
     5701 ctccataacg gaaccaatcg tctgatttct tttctgcaaa acctgtggta tcaaatgtct
     5761 ctgcgccctt ttctataaaa ccagaggaac gtatccctaa ttgtaaatct ttccactctt
     5821 ggttaaattc aatcttcact tggtataatt gtaataatcc atc
//
LOCUS       DAOOBB010000124         5849 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_15819_length_5849_cov_3.888505, whole genome shotgun sequence.
ACCESSION   DAOOBB010000124 DAOOBB010000000
VERSION     DAOOBB010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 5849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5849
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15819_length_5849_cov_3.888505"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /gene="galE"
                     /locus_tag="PLT31_06455"
     CDS             complement(<1..882)
                     /gene="galE"
                     /locus_tag="PLT31_06455"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HPW94816.1"
                     /translation="MKIAVIGGAGYIGSHTVRELLDQGHQVAVFDNLSSGLTQNLFSE
                     ASFFEGDILNTSALHSFFSEFKPEGIVHLAAFKAAGESMIAPEKYSVNNISGSLNILN
                     EAVAAGVSYFVFSSSAAVYGAPKYMPVNEEHPTDPENYYGYTKLAIEQYLSWYDKLKG
                     LKFAALRYFNAAGYDIQGRLTGLEKNPANLIPVVMEALIGKRKELLVFGNDYDTPDGT
                     GVRDYIHVNDLAVGHGMAFDQLQKTNTSFIVNLGVQQGASVLDIIKMAEKVSGKKVPY
                     RIVGRRPGDPASLIADPS"
     gene            1172..2950
                     /locus_tag="PLT31_06460"
     CDS             1172..2950
                     /locus_tag="PLT31_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPW94817.1"
                     /translation="MQPEALRLSEITISNLRSIRRETFPLSHFTALIGYNNAGKTNIL
                     MGIRWLLSHFTLNISFFDDAKIPVEVEGLFEGISEAVLDRLGEEKAAVITPYLMHGAL
                     RVKKIQRIPGDLKENIEMMVFVPPQKRRQQRVWEYASVDFVRSFERLFPEPIAIWDFE
                     GNQAFTKLLHEIFKPLERRFGNEFNDLILRFSELLSPSSENRAEEIKTFDNGVNQKLH
                     PLFPSVKVELSIPTPTLETFLQSATLKVIDEDDGFERNIERMGAGSQRAIQMALVRYL
                     AEVKKHHNNHYLNRKLLLVDSPELFLHPQAVELVRVALKNLSNEGFQVVFATHSAQMV
                     TSEDVSTSLLIRKNRKRGTFMRKRMEDAVYQVVQDAPSQLQMLFSLSNSNELLFADYV
                     LLTEGKTEWRVLPALFEKVTGQSFALIKCALVRQGGVSNTRKSMQVLAAMDMPVRAIV
                     DLDYAFNNAVHDGFLDKKDPDLRVCMDLFRELSFQEKIRLVNGVPVTKHSKVTASQAF
                     AMMAAMKEAEKPLKNIHAKLRYKGIWIWTKGAIEEHLGLDGKNERCWTQFVERVKKGK
                     IESFVPDYASIEELCKWIVEGSRQAF"
     gene            3057..5066
                     /locus_tag="PLT31_06465"
     CDS             3057..5066
                     /locus_tag="PLT31_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HPW94818.1"
                     /translation="MKKTLCQVCLVIGLCAAVSAVAGPGLADGASKFVGNITTGGQVR
                     SDFGTYWNQITAENECKWASIEGTRGSYNFRGCRAAYDWAKNNGGHFKFHALVWGSQY
                     PNWLNGLSTEETKKAITAWFDAVKKEFPDLEMIDVVNEAIRTGTNSYHSGYGKNNNII
                     PALGGDNNGDYTFVTTAFKMARERWPNAILIYNDYNTFQWNKNEGIDLIKRIKAAGAP
                     VDAYGQQAHDMMTQGGGAGGTGGGGACLNYNTFKSTLQEIYDKTQIPLYISEYDISTA
                     DDNVQKQCYQEQITAFMEAPYVAGITLWGYIYGSTWIDHSGIIKNGQDRPAMTWLKDY
                     LSKNKGVNTTNLGGGGGITSPSTVRVEEQTTAVTKLTTSFEGSATFAIEGGDDAALFT
                     LSGSDLAFKAAPSFASPGDAGKDNIYKVKVKATAGDKTASLEMNVIVTQKQAAYNGTT
                     PTLPAKIEAEAFDVCGEECAYHDAEVENQGETTLRDGEGVDIVQIGTGSEYAIGYTTA
                     GEWLEYTVNVENEAVYTLTARVASGSENSSFRLFIGDQAITDTVKVPAGADWDTYSNV
                     EIKTTKLTKGTHVIKLAITGSYVNIDWLDLSDGTNGIANSLGFNTSDVKSYRIYNFKG
                     AYMGMFKASDLTSLKKQMEKSDIKAGAYMVRSLDGHTNKIIEVKR"
     gene            5416..>5849
                     /locus_tag="PLT31_06470"
     CDS             5416..>5849
                     /locus_tag="PLT31_06470"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HPW94819.1"
                     /translation="MKLNQILLIGLLLISSVFAAPVSETLQVGSDQRKMLTYVPSNLE
                     PGSPLIISIHGMDQDPTYQHNNTRWDQVADTARFAVVYPQSMQSGFSTWDINTKSTSN
                     KDIQFFLAIIDDMEERYQIDRNRVYLGGFSMGGMISYLAMGV"
BASE COUNT         1812 a          981 c         1281 g         1775 t
ORIGIN      
        1 agaaggatca gcaattagag aagcagggtc tccagggcgg cgacctacaa ttcgataagg
       61 gactttttta ccagaaactt tttctgccat cttaataatg tctaatacac ttgctccttg
      121 ttgaacgcct aagttcacaa tgaaactagt atttgttttt tggagttggt caaaagccat
      181 gccatggcca acagcaagat cattgacatg gatatagtca cgaacgcctg tgccatcagg
      241 ggtgtcgtaa tcgttgccaa aaactaaaag ctcttttcgc tttccaatta aggcttccat
      301 cacgacagga atcaagtttg ctggattttt ttctagccct gtaagccttc cttgaatgtc
      361 ataaccagcg gcattaaaat aacgaagagc tgcaaacttt aagcctttga gcttatcgta
      421 ccaagacaag tattgttcta tagcgagttt tgtatagccg taataatttt ctgggtcagt
      481 agggtgttct tcattgacgg gcatgtattt tggagcacca tagacagcag ccgagctaga
      541 gaaaacaaaa taggaaacac ctgcagcaac cgcttcgttc agaatgttta aagagcccga
      601 gatgttattc acgctgtatt tttctggagc aatcatagat tctccagccg ccttaaatgc
      661 cgctaaatgg actatccctt caggcttaaa ttcagagaaa aaggaatgga gtgctgatgt
      721 atttaggata tcgccttcaa aaaaagaggc ttctgagaat aaattttgag ttaatccaga
      781 ggataaatta tcgaaaacag ccacttggtg accttgatct aatagttctc taactgtgtg
      841 ggagccaatg tagccggcac cgccgatgac tgcaattttc atttttcctc cgtcattttg
      901 cttattatag caaaaaacga ctagtagaac ccttattttg agcaaaaaaa gcgaaaaaag
      961 ggcggggtgt tgtaaaagag tgcaacataa aatacaaagc agaaacgaca aatgatttag
     1021 gaacttgtat attcttaaaa gaaataaaaa aaatcccaat cccacactgg ggttctcctt
     1081 taaagggaga gccccttttt tattggtatg aattatcata gggatactga tcatttttcc
     1141 ttttttccct agatttgtgt attttcaata gatgcagcca gaagccctta gattaagtga
     1201 aataacgatt tctaatttac gttccattcg acgtgaaacg tttcctctta gccattttac
     1261 ggctttgatt ggttataata atgctggaaa aacgaatatt ttgatgggta ttcgctggct
     1321 tctttctcat tttaccttga atatttcttt ttttgatgat gctaagattc ctgtagaagt
     1381 ggaaggcttg tttgaaggga tttctgaagc ggtattggat cggttaggcg aagaaaaagc
     1441 cgcggtgata acaccctact tgatgcacgg ggcattacga gttaaaaaaa tccaacgtat
     1501 tcctggagat ttgaaagaaa atatagagat gatggtattt gtgcctcctc aaaagcgccg
     1561 tcagcaacgt gtatgggaat atgcttctgt tgattttgtt cggtcttttg agcgtctttt
     1621 tccagagcct attgctattt gggattttga aggaaatcaa gcttttacaa agttacttca
     1681 tgagatattc aagcccttag agcgccgctt tggtaacgaa tttaacgatt taattttaag
     1741 gtttagtgag cttttatctc cttctagcga aaatagagct gaagagataa aaacatttga
     1801 taacggggtg aatcaaaaac tacacccttt gtttcctagt gtaaaagtag agctatctat
     1861 tcctacgcct actcttgaaa catttttgca atcggccaca ttaaaagtga ttgatgaaga
     1921 tgatggcttt gaacgtaata ttgaaagaat gggagcaggt tcgcaacgtg ctattcagat
     1981 ggctcttgtt cgatatttag cagaagttaa aaagcatcat aataatcatt atttgaatcg
     2041 aaaactgtta ttagtggatt ctccagaatt attccttcac ccgcaggctg tagaacttgt
     2101 gagagtggcg ttaaaaaatc tttctaatga aggcttccag gtggtctttg ctacgcattc
     2161 tgctcagatg gttaccagtg aagatgtaag tacctctctt ttaattcgaa agaatcgcaa
     2221 acgcggtact tttatgagaa agcgaatgga agatgctgtt tatcaggtgg tacaagatgc
     2281 ccctagtcaa ttgcagatgc tattttctct ttctaatagt aatgaactct tatttgctga
     2341 ttatgttctt ttaacagagg gtaaaacgga atggagagtt ttgcctgctt tatttgaaaa
     2401 agtgacagga caatcttttg ctttaattaa atgtgcttta gtgcgtcagg gtggggtaag
     2461 caatactaga aaaagtatgc aggtgctcgc tgctatggat atgcccgtac gtgcgattgt
     2521 agatttggat tatgcattta ataatgcggt tcatgatgga tttttagata aaaaagaccc
     2581 cgatttgcga gtatgcatgg atttatttag agaactctct tttcaagaga aaattcgcct
     2641 tgtcaatggg gttccagtaa caaagcactc aaaagtcacc gcatctcagg cttttgcgat
     2701 gatggctgct atgaaagagg ctgaaaaacc actaaaaaat attcatgcaa agttaagata
     2761 caagggaatt tggatttgga ccaaaggggc aattgaagag catttgggtt tagatgggaa
     2821 aaatgaaagg tgctggactc agttcgtaga acgtgtaaaa aagggcaaaa tcgagagttt
     2881 tgtgccagat tatgcaagta ttgaagagtt gtgtaagtgg attgttgaag gaagtcgcca
     2941 agcattttga taagcatcta tggataaatt gtccatagaa agccttttag tatctaaaaa
     3001 aaagaatggt ttaagaggta ttttcataaa gggtcgaaac caaaggagat acttttatga
     3061 aaaagacttt gtgtcaggtg tgtttggtta tcgggctctg tgcagcagtt tctgctgtag
     3121 caggtcctgg gttagctgat ggggctagca aatttgtagg aaatattacc acaggtgggc
     3181 aggtcagaag tgattttggc acctattgga atcaaattac agccgaaaat gagtgtaaat
     3241 gggcttctat tgaaggaact agaggtagtt acaactttag aggttgtaga gcagcctatg
     3301 attgggcaaa aaataatggg gggcatttta agtttcatgc cctagtttgg ggatcgcaat
     3361 accctaactg gctcaatgga ttaagcactg aagaaacaaa aaaagctatt acagcatggt
     3421 ttgatgcagt taaaaaagaa tttccagacc tagaaatgat tgacgtggtt aatgaagcta
     3481 ttcgtacagg aacaaattca taccattctg gttacgggaa aaataataac attatcccag
     3541 ctcttggtgg tgataacaat ggtgactata cctttgtgac tactgctttt aagatggcaa
     3601 gagaacgttg gccgaatgct atcttgattt acaatgacta caatacattc caatggaata
     3661 agaatgaagg cattgatttg atcaagagga tcaaagcagc tggtgctcct gtagatgcct
     3721 acggacaaca agcacatgat atgatgactc aaggtggtgg tgctggcggt actggaggcg
     3781 gcggcgcttg tctcaattac aataccttta agagtactct tcaagagata tatgataaaa
     3841 cacagattcc tttatacatt agtgaatacg atatttcaac cgctgatgat aacgtacaga
     3901 aacaatgcta tcaagaacaa attactgcct tcatggaagc accatatgta gctggtatca
     3961 ctttatgggg atacatctat ggttcgactt ggatcgatca ctcaggtatt attaagaacg
     4021 gacaggatcg tcctgcaatg acatggttga aagactattt gtccaaaaac aagggtgtga
     4081 atactacaaa tttaggtggt ggaggtggaa ttacttcacc tagtacggtt cgcgttgaag
     4141 aacaaacaac cgctgttacc aaattaacaa catcctttga aggttccgct acgtttgcta
     4201 tcgaaggcgg tgatgatgca gctttattta ctctcagtgg tagtgattta gcatttaagg
     4261 ccgctccttc atttgcttct ccaggtgatg ctggtaaaga caatatttat aaagtgaaag
     4321 tgaaggcgac tgcaggtgat aaaactgctt ctttagaaat gaatgttatt gttactcaaa
     4381 agcaagccgc ttataacggt actacaccaa cacttcctgc aaaaatcgaa gcagaagcat
     4441 ttgatgtctg cggtgaagaa tgtgcttatc atgatgccga agttgaaaac caaggcgaga
     4501 caacacttcg tgacggtgaa ggcgttgaca ttgttcaaat tggaacagga tcagaatatg
     4561 ctattggata tacaacggct ggtgaatggc tcgaatatac agtgaatgta gaaaatgaag
     4621 ctgtatacac cttaactgca cgtgtagctt ctggaagtga aaattcttcc tttagattat
     4681 tcatcggtga tcaagcgatt actgatacag tcaaagttcc agctggcgct gactgggata
     4741 catatagcaa cgtggaaatc aaaacgacca agttgacaaa aggaacccat gtcattaagc
     4801 ttgctattac aggaagctat gtgaatattg attggttaga tctttctgat ggaacaaatg
     4861 gtattgcaaa ttcacttggc ttcaatactt cagatgttaa gtcatatcgt atctataact
     4921 tcaaaggcgc ttatatggga atgtttaagg cttctgattt aacttcattg aaaaaacaaa
     4981 tggaaaaatc agatattaaa gctggcgctt atatggttcg ttctttagat ggtcatacga
     5041 ataagatcat tgaagtaaaa agatagtgga ttttatttga atttctctca ataaaacacc
     5101 agacacaaac cacaaaaaga ttctcctaat ttaaggaggg tctttttgtg ttttcgtttt
     5161 catgatagac attcttgctt catcatggtt ggcttctttt tttatgatga aggtgagaga
     5221 aagattagtt tatcttgatt ggcgtgcagc tttgcagatg atgtaaacat ggtgttgttt
     5281 ttctatacaa aagatttata aagtaataaa gggtattaac ataataagtt atatagattt
     5341 ataaaagcgt tttaagaatt gtttcaaatt tgtttgggtg acatttgaac aatgacataa
     5401 taataaggag tttttatgaa gttaaatcaa atattactga ttggtttatt attaatttca
     5461 agtgtgtttg ctgcacctgt ttcagaaaca cttcaggtgg gaagtgatca acgaaagatg
     5521 ctcacctatg tgccttcgaa tcttgagcct ggatcgccat tgattatttc tatacatgga
     5581 atggatcaag atccaactta ccagcacaac aatactcgct gggaccaggt ggcggataca
     5641 gcccgttttg ctgtggtata cccacagtcc atgcaatctg gattttctac atgggatatt
     5701 aacaccaaga gtacatcgaa taaagatatt cagtttttcc tcgctattat tgatgatatg
     5761 gaagaacgtt atcaaattga tcgtaaccga gtttacctcg gaggattttc catgggcggc
     5821 atgatcagtt atcttgcaat gggtgttat
//
LOCUS       DAOOBB010000125         5812 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_15925_length_5812_cov_4.718082, whole genome shotgun sequence.
ACCESSION   DAOOBB010000125 DAOOBB010000000
VERSION     DAOOBB010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 5812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5812
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15925_length_5812_cov_4.718082"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..1315)
                     /locus_tag="PLT31_06475"
     CDS             complement(110..1315)
                     /locus_tag="PLT31_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94820.1"
                     /translation="MSKTKNILLYAFFLFHCLFFSSCSENKEGTLPVTSENGSMSTVH
                     LNAKTLPSLTLDSLVILATGTDTIHQVLYDLETPLEMQLFPNKPWHFYAGLYAQGGIL
                     MQEGIVEMELEAGEEFFIEIPLKAIVGFIYVEIPLGFENQMKISSGTLHLSSKKLNKT
                     YSLNQEGPIAYFSSEALPLNTKYYANMILKDDLGDTIFIFKDSISIYDNSPVFHWKLT
                     SLRSTVSLSLEAQVIESNDFYASFSNAKKRYPQNGEILFTEIYITDKSKSAEYIELFN
                     GSIDSLILDSCYISIKSSNSGTLIPENTVIRPSSFLTIGGDSVFNADIKISSFDLTNT
                     KQALVLQCHNQKIDSVHYSSSDTINIDTISIQKNKSAQLPLRFWNEKKDFSKWCLGNQ
                     SIGKDALCK"
     gene            1805..1987
                     /locus_tag="PLT31_06480"
     CDS             1805..1987
                     /locus_tag="PLT31_06480"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HPW94821.1"
                     /translation="MRKLTLSSTDIKIAGVCAGIAEFLQIDPTIVRIICVCSLLIFGF
                     FTVPAYLLLWFIIPRE"
     gene            complement(1989..2483)
                     /locus_tag="PLT31_06485"
     CDS             complement(1989..2483)
                     /locus_tag="PLT31_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HPW94822.1"
                     /translation="MHRDTFLLMPLNELLRACTIKGFQGSGPGGQHRNKTNTGVCLKL
                     NPYNLEIKSSESRSASENKTHALHRMQMALALEVREEPLPAEKLRFPGSQGRIQTSNP
                     LFPLFIAQVLDIVYANKGDTKPAAKAFDLSPTALTKILHLNKSVVERLRIMRESFGKP
                     KLRM"
     gene            2478..3188
                     /locus_tag="PLT31_06490"
     CDS             2478..3188
                     /locus_tag="PLT31_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94823.1"
                     /translation="MHNVKDTILKSLLVTLSIFLWSCASSGAANKTTATEKKESYQMI
                     QEEKQLLLENTFKEMLATIKRGVAPDSLLPYITEESRYWLDDLEQAALTESSEMVSAR
                     SFHEILAILSYRLFQREHLYKTEQYRMLYLITAKKGILELVTSLPLGPFEVKNDRGSL
                     GLAKSPKVPVILFVWDDISWRMDLKNSIPLITKGLESIGVKKDWSNTELALYLLEKEF
                     RYDFRNIDKSLLDPVPSI"
     gene            3193..3732
                     /locus_tag="PLT31_06495"
     CDS             3193..3732
                     /locus_tag="PLT31_06495"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPW94824.1"
                     /translation="MKKAWQFLSKKTILDSRWLRVDQEKCLLPNGKVIDDFYTMSQPD
                     WVLILARTKEGKWIIESQYRHGTQKASLEFPAGIINEGETPIEAAKRELQEEVAYGEG
                     KWSFLGEFPMNPDRHRGRFFVVFADGVISKGTTCFDDTEEIDLSLLSQSELEEKIKTG
                     EFNHPHQLAAFYVWQLKQL"
     gene            3711..4712
                     /locus_tag="PLT31_06500"
     CDS             3711..4712
                     /locus_tag="PLT31_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HPW94825.1"
                     /translation="MAIKTIMNIRLPILVTGLAGVPGYALYRYFLKLFPGQVYGIRPR
                     KNACVSGDTVYAFDAEETSSLEYVFETHRFGTVIDASGNCALKACECDTARSKLLNYA
                     QGVETARLAKQYNSVLIRISTDMVFSGASGLGGYVETDPKDPIHNYGKHQSEAEEAIQ
                     DLVPNVVILRVPLPMDYAPGGEAGAIDWISYRFRPGRPATLYTDEYRNPIYGDDLCRV
                     VEYILSHEFPSGIYHCGGKRLVSLYQIGQIINALGGYNPDLLIGCLRTEAGPLPPRVG
                     HLGINCTKLYSLLPDNFIRPWPFLDSLFPTDPLWHKTVDRTPWTKFDAVDQYLVRGE"
     gene            complement(4699..5514)
                     /locus_tag="PLT31_06505"
     CDS             complement(4699..5514)
                     /locus_tag="PLT31_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HPW94826.1"
                     /translation="MIESLLLGLLQGLAEFLPISSSGHLVLGKSLLGMKDVGMFFDVM
                     LHAGTLLSIFVFFRKKLLQIIVGIFKKEKESCQYAFYVILASIPTAIIGIGFKDALES
                     LFTNPRAVSIALLFTGTLLFLTRFAKQTDKEEFASMTWWKALVIGVVQGIACIPGISR
                     SGSTISAMLFLKVNRKFAGEFSFLMSIPAVGGAALLDFKHWLECQGADALMQSCQDAG
                     AFNIALLAGMVVAFASGLFALRWLITFVQKGKFHHFAWYVWVAGILGLLFIHP"
BASE COUNT         1876 a          958 c         1079 g         1899 t
ORIGIN      
        1 tggagaccca tttaaagata ccgctggccc ttcgttaaat attttgatta aattgatgac
       61 aatggtaagc gttgtttttg ctggtgtgat cgtaaaattt ggaggctttc tatttacata
      121 aggcgtcttt tccaatagat tgattaccta aacaccattt tgaaaaatct tttttttcat
      181 tccaaaagcg caaaggcaac tgagcgcttt tatttttttg aatgctaatg gtatcgatat
      241 ttatcgtatc agaagaacta taatgtacag aatcaatttt ttgattatgg cattgtaaaa
      301 caagagcttg ttttgtattt gtcaaatcaa aagaagaaat tttgatatct gcattgaata
      361 cgctatctcc tccaatggtt aaaaaagaac taggccgaat cacagtgttt tcagggatca
      421 gtgttcctga attactactt tttattgaaa tataacagct atctaaaatt agagaatcta
      481 ttgaaccatt aaaaagttct atgtattctg ctgatttaga cttatctgta atataaattt
      541 ctgtaaaaag gatttctcca ttttgtggat aacgtttttt tgcatttgaa aaactagcgt
      601 aaaaatcatt tgattcaatc acttgagctt caagggataa agaaactgta ctgcgaagag
      661 atgttaattt ccaatggaat acagggctat tatcataaat gcttatgcta tccttaaaaa
      721 taaaaatcgt atcgcctaag tcatctttaa gtatcatatt agcataatat tttgtgttta
      781 gaggaagagc ttcgcttgaa aaataggcga taggaccttc ttgatttaga gaataggttt
      841 tatttaattt tttagaactt aagtgtaatg ttccagaaga aattttcatt tgattttcaa
      901 aacctaaggg aatttcaaca taaataaaac caacaattgc ttttagggga atttcaataa
      961 agaattcctc tcctgcttct aactccattt caactatacc ttcttgcatt aaaataccgc
     1021 cttgagcata aaggccagca taaaaatgcc aaggtttatt tggaaataat tgcatttcta
     1081 aaggagtttc taaatcatat aatacttggt gaatcgtatc agttccagtg gcgagtatta
     1141 caaggctatc taaagtaaga cttggtaacg ttttagcatt taagtgtact gtactcatac
     1201 ttccattttc tgaagtcacg ggaagagttc cttctttatt ctcagaacaa gaagaaaaaa
     1261 acagacaatg aaataggaaa aatgcatata gtaaaatgtt ttttgttttt gacataatga
     1321 tctccattat aaggtgaatg aatcttttag cattactttt aaaaagttcg aatggagtga
     1381 ttgaaattcg aagatcatag tcgtatttga aatgatcttc attttatctt aaaagtattt
     1441 taagagactc ttctctttaa acgtttctta aataagaaag ttcaggtatt tttaaaaata
     1501 tttttataaa aattctattg cgtaattagc ctatagaact tttctttgtg tcgtttttgg
     1561 aaaaagatta aagacttatt tatggattgt tttaaaaaag tgattaaaaa atggactata
     1621 aggatcattt tacgttattc attgaacgta agcataaaac ttaaaattaa tttatgaaat
     1681 cattaatttt gaaatcgata ttgaaaatac atagaataag tttactgaaa cgttcttcgt
     1741 tcatatacaa atggatttct tttttttata ttaaatttta gttgactttt gaaaggagtt
     1801 aaaaatgcgc aaattaacct tatcttcaac agatattaaa atagctggtg tttgtgctgg
     1861 tattgcagag tttcttcaaa tagatcctac gattgttcgt attatttgtg tttgctccct
     1921 tttaatcttt ggctttttta cagtgccagc ctatttactt ttatggttta ttattccaag
     1981 ggaatagtct acattcgaag ctttggtttt ccgaaagatt ctcgcatgat tctgagtctt
     2041 tcaacaacac ttttatttag atgcaagatt ttagttaacg ctgtggggga gagatcaaaa
     2101 gcctttgctg caggtttagt atctccttta tttgcataaa caatatctaa tacttgggct
     2161 ataaaaagcg gaaaaagtgg attcgacgtt tgaattcggc cttgactccc tggaaatctc
     2221 aatttttctg ctggaagagg ctcttctcga acttcaagtg caagagccat ttgcattcgg
     2281 tgtagtgcat gggttttatt ttcagatgca ctcctgcttt ctgaagattt gatttctaaa
     2341 ttatacgggt tgagcttgag acaaactcca gtatttgttt tattgcgatg ttggcctcca
     2401 ggaccacttc cttggaaacc tttaatagta caagctctca aaagttcgtt taatggcata
     2461 agtaaaaagg tatcgcgatg cataatgtta aagatacaat tcttaaatcc ctattagtca
     2521 cattatccat ttttttatgg agttgtgcat cttctggggc tgcaaataaa accacagcta
     2581 ctgaaaaaaa agaatcttat caaatgattc aagaagaaaa gcaactcctt cttgaaaata
     2641 cttttaagga aatgcttgct accataaaaa gaggtgtggc tcctgattct ttacttcctt
     2701 acataacaga agaatctcgt tattggctcg atgatttaga acaagctgca ttaacagaat
     2761 caagtgaaat ggtttcagct cgttcttttc atgaaatact agcgatttta tcttatcgtc
     2821 tttttcaacg agaacattta tataagacag aacaatatcg aatgctctat ttaattacag
     2881 cgaaaaaagg aattttagaa ttagtcacat ctcttcctct tggtcctttt gaagtaaaaa
     2941 atgatcgtgg tagtttaggt ttagctaaaa gtccaaaagt ccctgtaatt ctctttgttt
     3001 gggatgatat tagttggaga atggatttaa aaaattctat tcctttaatt acaaaaggtc
     3061 tagaatccat aggtgttaaa aaggactgga gcaataccga gttagctctt tatctattgg
     3121 aaaaagaatt tcgttatgac tttagaaaca ttgataagtc tttattagac ccagttcctt
     3181 ctatttagtg atatgaaaaa agcttggcaa tttctttcca aaaagacgat tttagattct
     3241 cgttggcttc gagtcgatca agaaaaatgt ttacttccta atggcaaagt aatagatgac
     3301 ttttacacca tgtcacaacc agattgggtt cttatattag ctcgaacaaa agaagggaaa
     3361 tggattattg agtctcaata tcgccatgga actcaaaaag cttctttaga gtttcccgca
     3421 gggattatta atgaaggcga aacacctatt gaagcagcaa aaagagagct tcaagaagaa
     3481 gtggcctacg gagaagggaa atggtctttc ttaggcgaat tcccaatgaa tccagatcgt
     3541 catcgcgggc gattttttgt ggtgtttgcc gatggtgtaa tatcgaaagg aactacttgt
     3601 ttcgatgaca cagaagaaat agatctttct ctattatcac agtctgagtt agaagaaaaa
     3661 ataaaaacag gggaatttaa tcatccccat cagttagccg ctttttatgt gtggcaatta
     3721 aaacaattat gaatattcgt ttacctattt tagtgactgg acttgcagga gttcctggtt
     3781 acgctcttta tcgttatttt ttaaaacttt ttcctggtca agtttatgga attcgcccta
     3841 gaaaaaatgc ctgcgttagc ggtgataccg tctatgcttt tgatgctgaa gaaacctcat
     3901 ccttagaata cgtttttgag actcatcgct ttggaactgt tatagatgct agtggtaatt
     3961 gtgcattaaa ggcttgcgaa tgtgatacgg ctcgatctaa attactgaat tatgcacaag
     4021 gggtcgaaac ggctcgtctc gcgaagcaat acaactccgt tttaatacgt atttctacgg
     4081 acatggtatt tagcggagct tctggtttag gtggctatgt agaaacagat ccaaaagacc
     4141 caatccataa ttatggaaaa catcaatcag aagcagaaga agcgattcaa gatttagtgc
     4201 cgaacgtggt gattttaaga gtacctcttc ctatggatta tgctccaggc ggagaagctg
     4261 gtgctattga ttggatttcc tacagattta gaccaggaag accagctact ctttatacag
     4321 atgaatacag gaaccctatt tatggcgatg atctttgtcg ggtcgtcgag tacattctat
     4381 ctcatgagtt tccttcgggc atataccatt gtggagggaa acgtcttgtc tcgctttatc
     4441 agatagggca aattattaat gctttagggg gttataatcc agatttatta attggttgcc
     4501 ttaggactga agcagggcca ctccctccaa gagtcgggca tttagggatc aattgcacta
     4561 aattgtattc gcttcttcct gataatttta ttcgaccatg gcctttttta gattctcttt
     4621 tccctacaga tccactttgg cacaaaacag tcgatcgaac tccttggacg aagttcgatg
     4681 ctgtagatca atatttagtt aggggtgaat aaataggagc ccaaggattc ctgcgaccca
     4741 aacataccag gcaaagtgat ggaatttccc tttttgcaca aaggtaatga gccagcgaag
     4801 agcaaataaa ccacttgcaa acgcgacgac catgccagct agaagagcga tgttgaacgc
     4861 acccgcatct tggcaagatt gcataagagc gtcagcgcct tgacattcta gccagtgctt
     4921 aaaatcaaga agagcagcac caccaacggc aggaatactc attaaaaaag agaactcacc
     4981 agcaaatttc cgattgactt ttaagaatag catcgcacta atagtggaac cacttcggct
     5041 aattcctgga atacaagcaa tcccttgtac cactccaatg acaagagctt tccaccaagt
     5101 catagaggcg aattcttctt tgtctgtttg tttggcaaaa cgagttaaaa acaataaagt
     5161 cccagtgaag agtaaagcaa tagaaacagc tcttggattt gtgaataaag actctaaggc
     5221 atctttaaat ccaattccta taattgctgt tggaatagac gcaagaatca cataaaaagc
     5281 atattggcag ctttcttttt cttttttaaa aatcccaacg ataatttgga gtagtttttt
     5341 tctaaaaaag acaaaaatag agagaagagt gccagcatgg agcattacat caaaaaacat
     5401 cccaacatct ttcatgccaa gtaatgattt tcctaaaacg agatgccctg aactagaaat
     5461 aggtaaaaat tcagcaagtc cttgaagtaa tccgagtaag agtgattcaa tcataggcgt
     5521 taatatagta aatcgcaaca aataaataaa acagaaatct tggataaaag aaacaattag
     5581 ttgtaaggcg agtttgaaat cgtgattgta taaataaaaa acgttatttt gattgtggat
     5641 ctgtctgttt tctattttgt atgtataaaa aaggcagcga gatgaatcgc tgccttttct
     5701 atatggaaat tataaataga gactattcgt caaatgtgta gctgataggc ttggcttcgg
     5761 aaatagcggc ggtaccagct tcattgtatg gaagaacaat gaatttgaca ct
//
LOCUS       DAOOBB010000126         5759 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_16107_length_5759_cov_4.075912, whole genome shotgun sequence.
ACCESSION   DAOOBB010000126 DAOOBB010000000
VERSION     DAOOBB010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 5759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5759
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16107_length_5759_cov_4.075912"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(274..1728)
                     /locus_tag="PLT31_06510"
     CDS             complement(274..1728)
                     /locus_tag="PLT31_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HPW94827.1"
                     /translation="MNSRIFTLIAFITFFSTSFAETPLPPAEEIFKKMGFGINIGNTM
                     EVPGNPTGWGNKFPTKTYIDSVKAAGFSTIRIPCAWDSHAKNSVINQSWLDSVQIVVD
                     YSIDAGLVTILNIHWDGGWLENNLTDEKKNEVNAKQKAYWTQIATHFKDYSENLLFAS
                     ANEPATSDTNYINETKILETYHQTFVDAVRATGGNNESRTLIIQGPSTSIDRSVEVMP
                     VEKLPNDVIQNRLIFEVHYYDPYTYTLMNEPANWGAIVQPQYYWGTGTNLATGSDEVH
                     NCGYNAWANAMGDPCTASQMETSLSKMKTNYVDKGVPVIIGEFGANNRVGVITGSNYE
                     RHHKGRLEYYDAFMKAAYRNKVVPIAWDTGHEGENNMTIIRRQSEPDGAVFDIEVLNI
                     MRHAYGLDDYVNIPITRIEKQSKKVPRETLSHQGQWIFAQGTRAKIQLFDLNGNLIRE
                     SKTTGPENRLEIEGIHAGSYVAKCGNQVLLLKVK"
     gene            complement(2115..4205)
                     /locus_tag="PLT31_06515"
     CDS             complement(2115..4205)
                     /locus_tag="PLT31_06515"
                     /inference="COORDINATES: protein motif:HMM:NF013276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HPW94828.1"
                     /translation="MKRLFLVLFLVLFSANAFAITKAKFDFVVGVDGNFKQAMDAAKN
                     ANPTQQKRFYIFFPNGEYDIGSLTGDANQKTTFTTSNVSFIGQNTDSTVIYNKSSDEG
                     ISITATLYFYKTDNMYLQDLTIYNKANYGNTSSYSKTGRHVAVQEQGNKFIYKNVKLL
                     STQDTYYTKSSRTYWETGEIHGTTDYICGGGDVFFNQCRLYNLKKSAMTAPSTTSDWG
                     YVFHSCTIDGNVTGYTLGRSWNNAKAVFINTTMNILPDEKGWGEPMNSVPQVFAEYGS
                     KNSSGGSIDLSQRRKTYSLDGVTVNLNPVLTASQAAQYTVENVLSGTDQWKPNLLTKQ
                     VSAPKLTFEGNRITWQDNDSALCWVVLKNGKYYTNVTSPSIETTSFTSGDKIIVRAAN
                     SMGGLGAASLAVTVEQDSRPYYKINLSPSLGGSISQNPEGTSLPEGTSVTFTAAPLLG
                     WQLDHWAGVHSGTENSWTHNSLTQDISLEAFFMPIDKNKYEAVSGVYEKGAIFENKNT
                     GYSGDGYVNFSAENGSSVDIPIYSDNSTDAEIHLVYANGSTATRTLSVLLNNSIVLAT
                     LDFEPTADWSTWGNKTISLSLPQGLSTISLQTVNENDGPNIDALTIEPKTTAVANSKN
                     KPSFYSYDSKSKVLFINSDTIQSISVELFSLDGKRLYSQQNKKSISLQSLNQGVYIMK
                     ILDGQNSFYGLVSL"
     gene            complement(4218..>5759)
                     /gene="pelA"
                     /locus_tag="PLT31_06520"
     CDS             complement(4218..>5759)
                     /gene="pelA"
                     /locus_tag="PLT31_06520"
                     /EC_number="4.2.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF021041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030570 - pectate lyase activity [Evidence
                     IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HPW94829.1"
                     /translation="IQEMRLLAVRYKSTTNSSYKTAFKNSFSKAIGFLVAAQRSKGAW
                     PQMHPKRGNYSDQATYNDNAMVRVMVLAKDIVDGVAPFDSDIVTESDRTKMQQALNKA
                     VDFALKSQIYNNNVPTVWCAQHDTSSYAPVEARAYELPSKSGSESAGIVWFLMNWPDQ
                     TPEIQKAIKGALAWYKKTRVADMKYSSGNFVSSSGSSLWYRFYEVNNDNYFFCDRGGV
                     STKTQDITKLSDDRRYGYQWAGDYGSALLNVEASYLEAISSLSSSSSSTPSSSSSSSS
                     SSSSSTPSFSCGDKDCLIQIEGEDFCSADGIFEDKNAGFSGTGYVNPNNDLETSVSYP
                     ISASVAGDYTFYVRYANGTATARDAQIVTNENKITPIQFPSTSAWTSWSTIPISVSLP
                     QGHSFFIFDALTSDGLPNIDWIGSTDQKLTISSCTPTKIENKLNVTSRVSGHIQNQHL
                     YLRNIPNSNVQVRILTLNGKQLHLLHANSSNVVPLPSMKGGLYLFEVYDNKNQMGSFL
                     LPLIK"
BASE COUNT         1681 a         1089 c         1157 g         1832 t
ORIGIN      
        1 ccaaaagata caatcaaaaa tttattagaa aaaaaccttt aatatccttc aaattatttt
       61 actgctcaat aaattttgat cgatcatata cttgatcggt cttttttttt gatttttttt
      121 gaatgccttt attttgccca ttttggacaa attgtccatg attaaattaa ttaaggtcgt
      181 tttttcatta aaaaactcag tatttttaag tcatagttaa ttaaaagttt cccatcacac
      241 acctaaatgg ctgccttatg gcagccattt attttatttt acttttaaaa gtaatacttg
      301 gtttccgcat tttgctacat aggatccagc atgaatgcct tcgatttcta agcggttttc
      361 tggccctgtt gttttggatt cacgaatcag gttcccgttc aaatcaaata gttgaatttt
      421 tgctcgcgtg ccttgggcga aaatccactg gccttgatga gaaagcgttt cacgaggcac
      481 tttttttgac tgtttttcta tgcgggtgat tggaatattc acgtagtcat cgagtccgta
      541 agcgtgacgc atgatgttta atacttctat gtcaaaaacg gcgccgtcgg gttctgattg
      601 gcgacgaata atcgtcatat tattttctcc ctcgtgccct gtatcccatg cgatgggaac
      661 aaccttattt ctgtaagccg ccttcataaa ggcatcgtaa tattctaggc gacctttgtg
      721 atgacgctcg tagttgcttc cagtgattac gcctacacga ttgttggcgc caaattcgcc
      781 gataatcaca ggaacgcctt tgtctacgta attcgttttc atcttggata gtgatgtttc
      841 catttggctt gctgtgcaag ggtctcccat ggcgtttgcc catgcgttat aaccgcagtt
      901 gtggacctcg tcgctcccag tggcgagatt cgtgcctgtg ccccagtagt attgaggttg
      961 tacaattgca ccccagttgg caggttcgtt catcaatgta taagtatagg gatcataata
     1021 gtgtacttca aatatcaatc gattttgaat cacatcgttt ggaagttttt ctacaggcat
     1081 cacttccaca gagcggtcaa tacttgtaga aggcccttga ataatcaatg tgcgagattc
     1141 attgtttcca ccagtcgcac gcacggcatc tacaaacgtc tgatgatagg tttcaaggat
     1201 tttcgtttcg tttatgtagt tagtatcaga ggttgctggt tcattcgcgc ttgcaaacaa
     1261 aagattttca ctgtaatctt taaaatgcgt cgcaatctgt gtccagtagg ctttttgctt
     1321 ggcgttgact tcatttttct tttcgtcggt taggttgttt tcaagccagc caccgtccca
     1381 gtggatgttt aaaattgtca caagacctgc atctatagag taatcgacca caatttgcac
     1441 tgaatccagc caagattggt tgatcacgct gttctttgca tgagaatccc aagcgcatgg
     1501 aatgcgtatg gtgctaaaac cagccgcttt tacagaatcg atataggttt ttgttggaaa
     1561 tttattgccc catcccgttg ggtttcctgg aacttccatc gtattgccga tgttaatacc
     1621 aaaacccatc ttcttaaaga tttcctctgc tggagggagt ggagtttctg caaaagaggt
     1681 ggaaaagaag gtaataaatg caataagcgt aaaaatacgg gaattcataa gacctcaagg
     1741 aaagagatct ttcaaatata tcctagcaaa taatgtttta taatactttt tagcaattag
     1801 cgttgacaaa aaaacaatga ctataattaa aatcgtatca accgtatcta aaatagatgg
     1861 gaatttacat tttgatagtg ataaaatcag atgatcacac ggcttttgta tggctaagcg
     1921 tatgcatttc ttaaccttta cgaataacgt taacaatgaa tgttatgtag tttgaagagc
     1981 tgttagactt atttgatatg tatccgagtt ggcttgctat atcgattcta tttcaaagaa
     2041 aagaggctcc cttgatgaga acctcttttt gtataaatca atcaaggagg tgtgccttca
     2101 ttgattaaga aattttataa tgaaactaag ccataaaacg aattctgtcc atcaagaatt
     2161 ttcataatat ataccccttg attcaaggat tgaagagaaa tactcttttt attctgttga
     2221 gaatagagtc gtttcccatc caaggagaat aattctacgg agatagattg gatagtatca
     2281 gagtttataa aaagtacttt tgatttggaa tcataggagt agaaactagg cttatttttg
     2341 gagttcgcta cagcagtcgt tttgggctca atcgtaagag catcaatatt agggccatca
     2401 ttttcattta ccgtctgtag ggaaatggta cttaatcctt gtggtaatga taaagagatg
     2461 gttttatttc cccaagtact ccagtctgca gtgggctcaa aatcaagagt tgctaaaact
     2521 atagaattat tgagaagaac gctaagggtt cgagtggctg tagaaccatt tgcataaacc
     2581 aagtggattt ctgcatctgt actattatca ctatagattg ggatatccac agaagacccg
     2641 ttctctgcag aaaaattcac ataaccatct ccagaatatc cagtgttttt attttcaaaa
     2701 atggctccct tttcatatac accacttaca gcctcatatt tgtttttatc aataggcatg
     2761 aaaaaagcct ctaaagaaat atcttgagtg agtgaattat gagtccagga gttttctgtg
     2821 ccagaatgaa cgcctgccca gtggtctaat tgccaaccaa gaaggggagc agcagtaaac
     2881 gtcacggaag tcccttctgg taaagaggtt ccctcaggat tttgagaaat agaaccacca
     2941 agagatggtg aaaggttgat tttatagtaa gggcgagagt cttgctccac agtgaccgcg
     3001 agagaagcgg ccccaagacc acccatcgaa ttggctgctc ttacaatgat tttatctcct
     3061 gaagtaaaag aggttgtttc tatagaagga gaggttacgt ttgtataata cttaccattt
     3121 tttaagacta cccagcaaag cgcgctatcg ttatcttgcc aagtaatacg attgccttcg
     3181 aaagtgagtt ttggagcgct cacttgcttc gtgagtaaat ttggtttcca ttggtctgtt
     3241 ccactcaaca cattttcaac ggtgtattga gcggcttgag aagctgttaa aacgggatta
     3301 agatttacgg taacaccatc taatgaataa gttttccgtc tttggcttaa atcaatggaa
     3361 ccgccggaac tatttttgct gccgtattcg gcaaatactt ggggaacgga attcatgggt
     3421 tccccccatc ctttttcgtc agggagtata ttcatcgtcg tattaataaa aacggctttg
     3481 gcattattcc aagaacgtcc taaagtataa ccagtgacat ttccatcaat ggtacaggag
     3541 tgaaaaacat aaccccaatc agaagtcgtg gatggtgctg tcatcgcact tttctttaga
     3601 ttatacaatc gacattgatt aaaaaagaca tctccgcctc cacatatata atccgtggtg
     3661 ccatgaattt cacctgtttc ccagtaagta cgagaacttt tggtataata agtatcttga
     3721 gtacttaaaa gttttacgtt tttataaatg aatttattcc cttgttcttg gacagcgacg
     3781 tgtctccctg ttttagaata agaactagta tttccgtaat tggctttgtt gtagattgtc
     3841 agatcttgta aatacatgtt gtctgtttta taaaaataaa gcgttgccgt aatactgatg
     3901 ccttcatctg aagatttatt gtaaatcact gtgctatctg tattttgtcc aataaaagaa
     3961 acgttagatg tggtgaaggt ggttttttga ttcgcatctc cagtgagaga accaatatca
     4021 tattctccat tggggaagaa aatatagaag cgtttttgct gagtcgggtt tgcgttttta
     4081 gcggcatcca ttgcttgctt aaaattgcca tcaaccccta ctacaaagtc aaatttggct
     4141 ttagtgatgg caaaggcatt cgctgaaaat agaactaaga aaagaactaa aaataaacgt
     4201 ttcataaatg ttccttttta cttgattaag ggcaataaaa aggagcccat ctgattttta
     4261 ttatcataaa cttcaaataa atagagtcca cccttcatag aagggagcgg tactacgttt
     4321 gaactatttg catgcagtag atgaagctgc tttccattta aggttaaaat gcggacttgc
     4381 acattgctgt taggaatgtt tcttaaatag agatgctgat tttgaatatg cccacttaca
     4441 cggcttgtta cattgagctt attttcaatt tttgtagggg tacaagaact tattgttaat
     4501 ttttgatctg tagatccaat ccaatcgata ttaggaagcc catcgcttgt aagtgcatca
     4561 aaaataaaga aggaatgccc ttgaggaaga gagactgaaa ttggaattgt actccatgaa
     4621 gtccaagcag aggtagatgg aaattgaatt ggcgtgatct tattttcgtt tgttacgatt
     4681 tgggcgtctc tagcagtcgc tgttccatta gcgtatcgaa cataaaaagt ataatctccc
     4741 gccacagaag ctgaaatggg atacgaaacg cttgtttcta aatcattatt cggattgacg
     4801 taacctgttc ctgaaaaacc agcattttta tcttcaaaaa taccatcagc actgcaaaaa
     4861 tcttctcctt ctatttgaat caaacagtct ttatctccgc agctaaaaga tggggtggag
     4921 ctagaggaag acgaagagga agaagagcta gacgaaggcg tagacgaact agacgaagat
     4981 aaagaagaaa tggcttccag ataacttgct tctacattga gaagagcgga accataatcg
     5041 cctgcccatt gatagccgta acgacggtcg tcagaaagct tggtaatatc ttgcgtttta
     5101 gtggatactc ctcctcgatc gcaaaagaaa taattgtcat tattgacttc gtaaaaacga
     5161 taccacaggg aagagcccga agaactgacg aaattaccgc tagaatattt catatcggct
     5221 actctagtct ttttgtacca agcaagagcc cctttgatgg ctttttgaat ctcgggcgtt
     5281 tgatctggcc agttcattaa aaaccataca ataccagctg attcgctccc tgatttagaa
     5341 ggtaattcat aagctcttgc ttctacagga gcataactag atgtatcatg ctgagcacac
     5401 cagactgttg gcacattgtt gttataaatt tgagatttta aggcgaagtc aacggcttta
     5461 tttaaagctt gttgcatttt ggttcgatcg gattctgtca caatatctga atcaaatggg
     5521 gcaacaccat cgacaatgtc tttcgctaga accataacac gaaccatggc attatcattg
     5581 taggtggcct gatcggagta atttcctcgt ttaggatgca tctgaggcca tgcgccttta
     5641 gaacgttgag cggctactaa aaagcctata gctttgctaa aggaattctt aaaagctgtt
     5701 ttataactac tattcgttgt gcttttataa cgtacagcca aaagcctcat ttcttgaat
//
LOCUS       DAOOBB010000127         5753 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_16123_length_5753_cov_4.723938, whole genome shotgun sequence.
ACCESSION   DAOOBB010000127 DAOOBB010000000
VERSION     DAOOBB010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 5753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5753
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16123_length_5753_cov_4.723938"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="PLT31_06525"
     CDS             complement(<1..517)
                     /locus_tag="PLT31_06525"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HPW94830.1"
                     /translation="MSRIFANLNQIRDTQRRRRFHIGVILGVLFLILLLFVYLFIGRS
                     GYWLVDDDSFEHVSWVAILDGQTADMERSDYTLKIMKEGKADSVLILGRRVFRDKNNA
                     DYYADDFMRSGNIDSGRVFLFRHNDPSTLEEALSIVPWFKSKNVDTVLLITSAAASNR
                     AKHIFTTLAGEK"
     gene            complement(560..967)
                     /gene="rimI"
                     /locus_tag="PLT31_06530"
     CDS             complement(560..967)
                     /gene="rimI"
                     /locus_tag="PLT31_06530"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HPW94831.1"
                     /translation="MDLDFVIEIQNELGFQNWNKKQFESELASPLSHPLVLILNDKII
                     GYMFLQVIDEEAELLSIAIKSGYEKKGAGSFLWKEGVSSLKEKNVRSVFLEVRENNIT
                     AQLFYKKHGFINIGIRKHYYSDGENALLFRLDF"
     gene            complement(993..1637)
                     /gene="tsaB"
                     /locus_tag="PLT31_06535"
     CDS             complement(993..1637)
                     /gene="tsaB"
                     /locus_tag="PLT31_06535"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HPW94832.1"
                     /translation="MANDSFDLVVDSSRYGIAMGLYQNNQRFEFFDNKARGESAGHVL
                     DQLLEQANTSLDHVKRVLVTLGPGSFTGLRTGIAFCQGLCFSGKRELYGISTLEALTS
                     LSKNPNNAVIIRARPGYWYLRYQNKEVFYSTQEVLSTLNSSNVLSDLIIDSFASEDLE
                     IKNFIEQKKQEISLEVTLISGTELESFLVFLDQLKPSLIQEANYIQPSYFEKKS"
     gene            complement(1637..2671)
                     /locus_tag="PLT31_06540"
     CDS             complement(1637..2671)
                     /locus_tag="PLT31_06540"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HPW94833.1"
                     /translation="MSRLRVLVFMGGFSTEHDVSVVSGTGIVRAMDPQKYNIHPVLIE
                     KNGTWIWSSRELSPYQKENFSLNYFHSLDGKSACKKRAPALSELPDCDLVFLALHGKW
                     GEDGHVQALLEHWDMPYTGCGVLASALAMDKIKTKEIYQINGISTPDYEVLHKDSFSG
                     DQLVSVADRLGYPLVIKDPLGGSSLGIGIAANLEEAGTICSSLFNNTKVLLCEKFIKG
                     REASCGYIEGEKPVPPTELRMTTREYFDFDAKYNGECREITPAEFPEPLTREIQELVK
                     KAHLALGCDVYSRTDVLIDHQSKVWAIETNTLPGFTPTSLLPQQAACNGISYSQLIDL
                     IIEKSLKVKR"
     gene            2957..3877
                     /locus_tag="PLT31_06545"
     CDS             2957..3877
                     /locus_tag="PLT31_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPW94834.1"
                     /translation="MRVFLTGGTGFIGHYVAKKLIEQGHEISIATRHPDKIPSLKGTP
                     GVTMVESALLDLTSLERGLKGCDACIHIALGWGETPFSMLENDTRATVQILELAAMAG
                     CKKFIYTSSTAAMGKMRPVMNETVANLPIDLYGATKAAGEAYVLGFRQGYGNKFSSVE
                     MSRNIIRPGYTFGNPAFPDGCTQPDRRFAEMASAIKNGRDVRIVKNDGTQFIHASQQA
                     ELYVKLLESNYNEEIFMGLSNTWISWKSIAEKMLSLCPKSKSKIIEADLGWSDKPMLY
                     SVEKIARCFNLVFDSQSLIDEHIQYWIDRV"
     gene            complement(3880..4350)
                     /locus_tag="PLT31_06550"
     CDS             complement(3880..4350)
                     /locus_tag="PLT31_06550"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPW94835.1"
                     /translation="MISESTFSLNSIASFSEEHVHIWKEPISQQIALGLLFDLWASSN
                     ESLNPVDVWQKIISRNATEGFYVGSGLVLPHARVQGIEKSFLAFGVCPKGFEGVQKTK
                     GELATIMCLLFSPEKDPMIHMYVIKHIAQKLMDAQWKKRILNSLDAFEVKELLA"
     gene            complement(4347..5366)
                     /locus_tag="PLT31_06555"
     CDS             complement(4347..5366)
                     /locus_tag="PLT31_06555"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HPW94836.1"
                     /translation="MLRTTKLISPFIKEFLLSHGYSDESCVESAGSAGSGRLYYRIKQ
                     YKRSSILQVNAESNEDFKNFVSFGKYFHSINLPVPRIYSVDEKNSQVLMEDLGKRSLF
                     DYTLPEEPLSGNVRILYQDVIQALIKFQESSSSVFMNRPDIGARKFDYAALKWETDYF
                     STNYLKRIAKVDEIPSYVQEFFSTLACSVDSHQKVLMHRDFQSQNIMIRPNSEIAFVD
                     FQGARRGSMYYDLASLLFDPYTSLPLFLVIDFFEYWRTDFSENKSFSKEDSWVSFLEA
                     AMQRIMQALGAYGFLSKEKGLTTFEQFIEPGKARLIEILDLYQDVAPEIHKQARLFLK
                     EKLSI"
BASE COUNT         1956 a         1072 c          956 g         1769 t
ORIGIN      
        1 gcttctctcc agcgagcgta gtgaaaatat gttttgctcg atttgatgcc gctgcagaag
       61 taatgagtaa aacggtatct acatttttag acttaaacca tggaacaata gaaagagctt
      121 cttctagagt agatgggtca ttatgcctaa aaagaaaaac acgcccacta tctatgtttc
      181 cagagcgcat aaaatcatcc gcataataat ccgcattatt tttatctcga aaaacccttc
      241 gtcctaagat taaaacggag tcagctttgc cttctttcat tatttttaaa gtataatcag
      301 aacgttccat atctgcagtt tgtccatcta aaatagcgac ccaagaaaca tgttcaaagg
      361 aatcatcatc tacaagccaa taaccacttc gacctataaa taaatagaca aataacagga
      421 gaattaaaaa tagaacccca agaataacac ctatatgaaa gcgacgacga cgctgagtat
      481 cacgaatttg attaagattt gcaaaaatac ggctcaaaag tataccctta ttaaataact
      541 tctataatct aataaataat taaaaatcta accggaacaa aagtgcgttt tcaccatcag
      601 aataatagtg ttttctaatt ccaatattta taaaaccatg ctttttataa aatagctgag
      661 ctgttatatt gttttcacga acttctaaga acacagaccg aacatttttt tcttttaaag
      721 aagaaacacc ttctttccat aaaaaagaac cagccccctt cttttcataa cccgatttaa
      781 tggcaatact taaaagttca gcctcttcgt caatcacctg taaaaacata tagccaatta
      841 tcttatcatt taaaatcaaa accagcggat gcgataaagg ggaagctaat tctgattcga
      901 attgtttttt attccagttt tgaaagccta actcattttg aatttctatt acaaaatcta
      961 aatccaaaaa gttcatcttt cggcaaatca tatcaagatt tcttttcaaa ataagagggt
     1021 tgaatgtaat ttgcttcttg aattaatgag ggttttaatt gatctaaaaa gacaagaaaa
     1081 gattctaatt ctgttccaga aatcagcgtt acttctagcg agatttcttg ttttttctgt
     1141 tctataaagt ttttaatttc gagatcttct gatgcaaaag aatcgataat aagatccgat
     1201 aaaacgttcg atgagtttaa ggtagaaagt acttcttgtg tggaataaaa tacttcttta
     1261 ttttgatacc gtaaatacca ataaccaggg cgcgctctaa tgataacggc attatttgga
     1321 tttttggata atgacgttaa agcttcaaga gtagaaatcc cataaagctc tcgcttacca
     1381 gagaagcata gcccttgaca aaaggcaatg cctgtacgaa gccctgtaaa agaacctggg
     1441 cctaaagtaa cgagaacgcg tttcacatga tctaaagatg tattcgcttg ctcaagaagt
     1501 tgatctaaaa catgaccagc agattctccc ctagctttat tatcaaaaaa ctcaaaacgc
     1561 tgattgtttt gataaaggcc catggctata ccatatcgag aagaatccac cactaaatca
     1621 aaagaatcat ttgccattag cgtttgacct ttaaactttt ctcaataatc aaatcaatca
     1681 attgcgaata agaaatccca ttacaagcgg cttgctgcgg gagcaatgac gttggtgtaa
     1741 aaccaggaag agtattggtt tcgatggccc atacttttga ctgatgatca attaaaacat
     1801 ctgtacggct ataaacgtca cagcctaaag caagatgagc ttttttaaca agttcttgaa
     1861 tttctcgagt taatggttca gggaattccg caggtgttat ttcacgacat tcgccattgt
     1921 atttggcatc aaaatcaaaa tattccctag tcgtcatacg caattctgtc ggaggcacag
     1981 gcttttcgcc ctcaatgtaa ccgcaactag cctctctacc tttaataaac ttttcgcata
     2041 aaaggacttt cgtattgtta aatagagaag aacaaatggt tcccgcttct tctagattag
     2101 cagcgattcc aataccaagg cttgagcctc ctaaaggatc tttgataacc aagggataac
     2161 ctaaacgatc tgctacagat actaattgat cgccagaaaa cgagtcttta tgcaaaactt
     2221 cataatctgg agtagaaata ccattgattt gataaatttc tttagtttta attttatcca
     2281 tcgctagagc agaggcaaga acaccacaac cagtgtaagg catatcccag tgttctaaaa
     2341 gagcttgaac atggccatct tcaccccact taccatggag agcgagaaaa acaagatcac
     2401 aatcagggag ttccgataat gcgggcgctc tttttttgca cgcggatttc ccgtctaatg
     2461 aatgaaaata attcaaagaa aaattttctt tttggtatgg gcttagctct cttgaagacc
     2521 aaatccaagt gccatttttt tctattaaaa caggatggat attgtatttt tgaggatcca
     2581 tagcacgtac aatgccagta ccactaacaa cggaaacgtc atgttctgtt gaaaagccac
     2641 ccatgaaaac caaaacgcgc aaacgagaca taaataatac ctataaaaaa gtcaacgaat
     2701 aaagtatagt tttttcttcg attttaggct ttcggatttt ataatctttc aaatatttaa
     2761 tgattcaata aaagcactca caaataaact tttttacaga taatcataag cataaaaaaa
     2821 caaataacct aagtgattga atttgaatga attagatatt aatcactcta aaaagatcct
     2881 ttattttttt tatacctttt tttcataaat ttatttaggt tattaaggtt ctttttttct
     2941 ctttggatgg tagattatgc gtgtattttt aactggtgga acaggattta ttggtcatta
     3001 cgtggccaaa aaacttattg aacaaggtca tgaaatttct atagccacaa ggcatccaga
     3061 taagattcct tctttaaaag gaactcctgg cgtaacaatg gtagagtccg ctttacttga
     3121 tttaacttca ttagaaagag gtttgaaagg ttgcgacgcc tgtattcata ttgctcttgg
     3181 atggggtgaa acaccatttt ctatgttaga aaacgacacc cgagccactg ttcaaattct
     3241 tgagcttgca gccatggctg gatgtaaaaa gtttatttat acctcaagta ccgctgctat
     3301 gggaaaaatg aggcccgtca tgaacgaaac tgtagcgaac ttgcctattg atctctatgg
     3361 agccacaaaa gcagctggag aagcctatgt cttaggattt aggcaagggt atggtaataa
     3421 attttcatct gttgaaatgt ctcgaaatat tattcgtcct ggttatactt ttggaaaccc
     3481 agcttttcct gacggctgta ctcaaccaga tcgccgattt gcagaaatgg ctagtgcaat
     3541 taaaaatggc cgtgatgttc gaatcgttaa aaatgatgga actcaattca ttcatgcatc
     3601 gcaacaagct gaactatatg taaagctcct agaatccaat tataacgaag aaatatttat
     3661 gggattaagt aatacttgga ttagctggaa atcgatcgct gaaaaaatgc tttctctttg
     3721 ccctaaatcc aagtcaaaga ttattgaagc cgatttaggt tggtctgata aaccaatgct
     3781 ttattccgta gaaaaaattg caaggtgttt taatcttgtt ttcgatagcc aatcattaat
     3841 tgatgagcat attcaatact ggatagatcg agtttaattt taagctaata attctttgac
     3901 ttcaaacgca tctaatgaat ttaagatgcg ttttttccat tgtgcatcca ttaatttttg
     3961 agcgatatgc ttaatgacat acatgtgaat catcggatct ttttctggag aaaataaaag
     4021 gcacataatc gttgccagct ctccctttgt tttttgaaca ccttcaaaac ccttaggaca
     4081 aacaccaaat gccaaaaatg acttttctat tccttggact cttgcatgag gcaatactaa
     4141 cccactgcct acataaaatc cttccgttgc atttctagag attatctttt gccaaacgtc
     4201 cacagggttt aagctctcat tagacgaggc ccacaaatca aatagcaacc caagcgcaat
     4261 ttgttggctg atgggttctt tccagatatg aacgtgttct tcagagaaag aggctataga
     4321 atttaaggaa aaagtggatt cagaaatcat atacttaatt tttcttttaa gaagagcctt
     4381 gcttgtttgt gaatctctgg agcgacatct tgataaagat ctagaatctc aattaaacga
     4441 gctttaccag gttctataaa ttgttcaaaa gtagttagcc ctttttcttt tgataagaat
     4501 ccataagcac caagagcctg cattatacgt tgcattgcag cttctaaaaa agagacccaa
     4561 ctatcttctt ttgaaaaaga tttgttttca gaaaaatctg tgcgccaata ttcaaaaaaa
     4621 tcaatcacta aaaaaagtgg caatgatgtg tatggatcaa aaagtaatga agctaaatca
     4681 taatacattg atccgcgacg agcaccctga aaatcaacaa aagcgatttc tgaattaggt
     4741 cggatcatga tgttttgaga ttgaaaatct ctgtgcatca atactttctg atgagaatcc
     4801 acagaacacg ctaaagtaga gaaaaactcc tgaacataag aaggaatttc atcaactttc
     4861 gcaatacgct taaggtaatt agttgagaaa taatctgttt cccattttaa tgccgcataa
     4921 tcaaatttac gagcccctat atcaggacga ttcataaaaa cagaagaaga ggactcttga
     4981 aacttgatta aagcttgaat cacatcttga tataaaatac gaacattgcc agataaaggt
     5041 tcttctggta atgtgtaatc aaaaagagat cgtttcccta gatcttccat taagacttga
     5101 gaattttttt cgtccaccga gtaaatacgt ggaacaggta aattaatgct gtgaaaatat
     5161 ttaccaaaag aaacgaagtt cttaaaatct tcatttgact ctgcattaac ctgaagaatc
     5221 gatgatcttt tatattgttt aattctataa tacaaacgtc ctgaaccagc ggatccagcg
     5281 gattcaacac aagattcatc agaataacca tgagagagaa gaaactcttt aataaatgga
     5341 cttattagtt tagtagttct aagcatagcc tacaatatac tctgaattaa taaagaaaat
     5401 gatcctgtaa ttaaaaaaaa aggggcgatt tttgaaaaat gtatgttaaa acacttatgg
     5461 tcaccattaa taattatttt tcaaaaattt gatgcactac atcaccaaaa tttgtatttc
     5521 attgtggcga atacgaccaa catcccctac ttttcgttct tgcttttatt tcaatgcaat
     5581 cgaaatttat tatccaaaac cctttaaata gggaacctac tattgtttta gaagaaacgc
     5641 ctattttatt tttcttttct agctggactg cgagctaagc tacgagctac cccaaataaa
     5701 ccacccaatt cattttctga aggttgaagt cgttgtaaaa gcaaacgaat tcc
//
LOCUS       DAOOBB010000128         5752 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_16126_length_5752_cov_4.679831, whole genome shotgun sequence.
ACCESSION   DAOOBB010000128 DAOOBB010000000
VERSION     DAOOBB010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 5752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5752
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16126_length_5752_cov_4.679831"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PLT31_06560"
     CDS             complement(<1..252)
                     /locus_tag="PLT31_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94837.1"
                     /translation="MTIFWVAFLLLVIGMLALDLGVFHKEDKIISVKEAFLWTGIWIS
                     VSLIFSIGIFFIYHYQDAALGAEKVLEYLTAYVVEKSLSL"
     gene            469..1605
                     /gene="tgt"
                     /locus_tag="PLT31_06565"
     CDS             469..1605
                     /gene="tgt"
                     /locus_tag="PLT31_06565"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPW94838.1"
                     /translation="MNPFQLLKTSPRSKARLGKITTAHGEIPTPIFMPVGTAASVKAV
                     TPRDLKEMEAKIVLANTYHLYLRPGTPLIAKTGGIHRFMNWDGPVLTDSGGFQVWSLK
                     ELRKIKENGVEFRSHIDGSKHFFSPESVMKAQREIGADIIMAFDECTPYPSTPKEAEH
                     SLNFTLRWTKEALEYLQKNPPLHGYDQHFFGIIQGGMHKNLRQLAIERITELNPDGYA
                     LGGLSVGEPTDVMYDIADFCTDFMPKDHARYVMGVGTPWNLLELIGRGVDMCDCVMPS
                     RNARNGMLFTSQGVLHYKAGRYSQITDEAPDPECDCYTCRNFSKGYLRHLHQSGEILA
                     MTLSTIHNLHFYLKLMEQARNHISADTFEPWAAEQIIKLQQQVP"
     gene            1700..2719
                     /locus_tag="PLT31_06570"
     CDS             1700..2719
                     /locus_tag="PLT31_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014544982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HPW94839.1"
                     /translation="MSEKLTLEFIDPVPMRYGENSHQKAVFYKEPNCDEASLATAKQL
                     HGKELSFNNIVDADAALEMAKEFHNDTCVVIVKHMNPCGLATGETLEEAFEAAWAGDP
                     VSAFGSVIAVTKKVDLKAATFLKGRFVEILIAPAFDDDALEFLKHKSKDIRILEVGKL
                     EAPKARKVYKHVIGGLLVQDRDIGTWDKFECVTKAQFPPNKEALARFTWIVTKHTKSN
                     AIVMGYEYKPGYFQIMGLGPGQPNRIDSNLRLCQPRVRDNVARLPLPEGTTLEAMEQK
                     VFSEVVMGSDAFFPFSDNIDACASAGVRYIVQPGGSVRDPEVIDACNKLDIAMVFTGM
                     RHFRH"
     gene            2719..3162
                     /locus_tag="PLT31_06575"
     CDS             2719..3162
                     /locus_tag="PLT31_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94840.1"
                     /translation="MSIKKNISLDKKAYDTVLGFLKKIHKTGKIEAMDWNEFRMNLEK
                     VIVQEEQVKAIDLWTMENLEQHHPEMIRPRVLHEFESTFISPKSHGLFMEALHYGMVS
                     QSQGEMIIDELLDSDALGLLPDTMENSLAKTWKKNTAIYNKVRLN"
     gene            3164..4495
                     /gene="trmFO"
                     /locus_tag="PLT31_06580"
     CDS             3164..4495
                     /gene="trmFO"
                     /locus_tag="PLT31_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014544980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HPW94841.1"
                     /translation="MQKTRVKVIGGGLAGCEAALQLASYGFLIDLYEMRPKVNTPAHR
                     GGNLAQLVCSNSFKGIAKTTAHGLLKNELTLLGSFLIELAKEAQVPAGESLTVNREIF
                     SALVEKKISECPAITLHREEVTSLEDDLPTLVAAGPLASEALSNSIFNALGSERLHFF
                     DAIAPVVEADSIDFNHAFYMNRWEKGETADFINCPLDVETYNAFVEKLREADSVEPRP
                     FEKNELFEGCLPIEEMARRGTETLRYGPMRPIGLGFGNEGKKWHAVIQLRAENAQKTL
                     FNMVGFQTRLKWNTQKELFAMVPALKNARFARLGCMHRNTFIESPKLLEPTLQLKRGL
                     PGCEKLPPIWFAGQITGAEGYTEAVATGWYAAWNMACSLKHGVPQLLPKESCIRSLVD
                     QLVSPNPDFQPMNFNFGLLPHLIEMKKKNKKEILGNQAEEAVIEWIRKKPY"
     gene            complement(4516..4588)
                     /locus_tag="PLT31_06585"
     tRNA            complement(4516..4588)
                     /locus_tag="PLT31_06585"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4552..4554),aa:Arg,seq:cct)
     gene            complement(4648..5475)
                     /gene="dapB"
                     /locus_tag="PLT31_06590"
     CDS             complement(4648..5475)
                     /gene="dapB"
                     /locus_tag="PLT31_06590"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HPW94842.1"
                     /translation="MSVYVMVNGIPGNMGCMVAETCVARGLKLVPYSLTGEIVEEKEK
                     MVAGVKVELKPSTRENIIADVMKRYPDMICIDYTHPTAVNDNAAFYVKHGIPFVMGTT
                     GGDRETLQKLVEENNHPSVIAPNMAKQIVAFQTMIEFLANEFPTAFSDYKLSVVESHQ
                     KTKADTSGTAKAVVASFQKMGFDFSEKDIELVRDEKQQLERMHVPQDALSGHAFHTYS
                     LDSADGSVHFEFQHNVCGRHVYAEGTVDAVAFLAEKLKEGKARPFNMMDVLRSGKMR"
     gene            complement(5509..>5752)
                     /locus_tag="PLT31_06595"
     CDS             complement(5509..>5752)
                     /locus_tag="PLT31_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HPW94843.1"
                     /translation="EIKDDATIVARLLNRAKIEGRKDDADESIIKNRLVEYREKTAAV
                     LSKYDEKIVTRIVGDNSPDEVFCDTLQAYIRFTKNP"
BASE COUNT         1784 a         1150 c         1121 g         1697 t
ORIGIN      
        1 taaagataaa gacttttcaa cgacataggc cgttaggtat tcaagaactt tctctgcacc
       61 taaagcagca tcttgataat ggtatataaa gaaaatacct atgctaaaaa tcaatgagac
      121 ggagatccaa atcccagtcc ataaaaaagc ttctttaaca cttattattt tatcttcttt
      181 gtgaaagaca cccaaatcta aggcaagcat acctatgact aagagtaaaa atgcaaccca
      241 gaatattgtc atgggtttaa aatagcaaat aaaaaataaa acggtagaac aactcatctg
      301 atttattgac atggaaaaaa atccatttta ttctctcaat attaaaagga taagtcccaa
      361 aaccctcatt ttttcttttc tgcatcattt tggttattaa aaatcatttc gcaaaagcat
      421 tttatagagg acccaaaaga aaagttaaac tttttctatc tttaaaaaat gaatccattt
      481 caactactta aaacctcccc tcgctctaaa gcccggctag gcaaaataac aacggctcat
      541 ggagaaatcc ccactcccat ttttatgccc gttggtactg cagcaagtgt taaagcggta
      601 acgcctcgtg atctcaaaga aatggaagct aaaattgttt tagcgaacac ctatcatctt
      661 tacctaaggc caggaactcc ccttattgca aaaacaggcg gcattcatcg ctttatgaac
      721 tgggatggtc ctgtgttaac agatagcgga ggctttcagg tttggagtct aaaagagctt
      781 cgtaagatta aagaaaatgg cgtcgagttt agaagccata tcgatggatc taaacacttc
      841 ttttcccctg aaagtgtgat gaaagcgcaa agagaaattg gggccgatat catcatggct
      901 tttgatgaat gtacacccta cccaagtact ccaaaagaag cggaacattc cttaaacttc
      961 actttacgct ggactaaaga ggctttagaa tatcttcaaa agaatccccc tttgcatggt
     1021 tatgatcaac attttttcgg tatcattcaa ggcggaatgc ataagaattt aagacagctc
     1081 gcgattgaac gcattacaga actgaaccca gatggctatg ccctaggcgg cctatccgtt
     1141 ggagaaccta ccgatgtaat gtatgacatt gctgattttt gtactgattt tatgcctaaa
     1201 gatcacgctc gttatgtaat gggtgtgggg actccatgga atttactgga attaattgga
     1261 cgtggcgtag atatgtgcga ttgcgtaatg cctagtcgta acgcgagaaa cggcatgctc
     1321 tttacaagcc aaggggtttt acactataaa gcagggcgtt attctcagat cacagacgaa
     1381 gctcctgatc ctgagtgcga ttgttatact tgcagaaatt ttagtaaagg gtatttacgt
     1441 cacttgcacc aatctggaga gattcttgcg atgactttat ctaccattca taatcttcat
     1501 ttttacttga agcttatgga acaagcaaga aatcacattt ctgccgatac ctttgagcct
     1561 tgggccgctg agcaaataat taagctacaa caacaggtgc catagattat agggttatat
     1621 ttctctttga aagtaaaata aaactttaaa aaaatacagg tttactaaat tttaattgtc
     1681 atttattacc gaggaagtta tgtcagaaaa actcacgctt gaatttattg atccagttcc
     1741 catgcgctac ggcgaaaact ctcaccaaaa agcagttttt tataaagaac caaactgcga
     1801 tgaagcaagc cttgccacag caaaacaact tcatgggaaa gaactttcct ttaataatat
     1861 tgtcgatgca gatgccgctc ttgaaatggc aaaagaattt cacaatgaca cctgtgttgt
     1921 cattgtgaaa cacatgaacc cttgcggtct tgctacaggc gaaaccctag aagaagcttt
     1981 tgaagccgct tgggctggag accccgtatc ggcttttggt tctgttatag cagtcaccaa
     2041 aaaagtagac ttaaaagccg cgactttttt gaaaggacgt tttgtcgaaa ttcttattgc
     2101 gcctgctttt gatgacgacg ctcttgagtt tttaaaacat aaatccaaag atattcgtat
     2161 tcttgaagta ggcaaattag aagctcctaa agcaagaaaa gtatataagc atgtgattgg
     2221 cggattactc gttcaagacc gtgacatcgg tacttgggac aagtttgaat gtgtcactaa
     2281 agcccaattt cctcctaaca aagaagctct tgcccgcttt acatggattg taacaaagca
     2341 taccaaatcc aatgcaatcg tgatgggtta tgaatataaa cctggttatt tccaaatcat
     2401 ggggcttgga cctggtcaac caaatcgtat tgactccaat ttaagattat gccaacctcg
     2461 cgtaagagac aacgtcgctc gccttccact tcctgaaggg accactcttg aagcgatgga
     2521 acaaaaggta tttagcgaag tcgttatggg ttccgatgcc ttcttcccat tctctgataa
     2581 tattgacgct tgtgcttcag caggagttcg ttatattgta cagcctggtg gctctgtaag
     2641 agatcctgaa gtgattgacg cttgtaataa actagacatt gccatggtct ttactggtat
     2701 gaggcatttt agacactaat gagtattaaa aaaaatattt ctttagataa aaaagcatac
     2761 gataccgttc ttggatttct aaaaaaaatt cataaaacgg gtaaaattga agccatggat
     2821 tggaatgaat ttcgtatgaa tctagaaaaa gtcattgttc aagaagagca agtgaaggcc
     2881 attgatttat ggaccatgga gaacttggaa caacatcatc ctgaaatgat tcgcccacgt
     2941 gttttacatg agtttgaatc gacctttatt tcgcccaaat cacatggctt atttatggaa
     3001 gctcttcatt atgggatggt tagccagtct caaggagaga tgattataga tgagttatta
     3061 gactccgatg ctcttggttt attaccagat acgatggaaa attctttagc caaaacttgg
     3121 aaaaagaata cggccattta caacaaagta cggttaaatt aatatgcaaa aaactcgggt
     3181 caaagtcatt ggcggcggtc ttgctggctg tgaagcagct cttcaactag cctcctacgg
     3241 atttcttatt gatctctatg agatgagacc taaggtaaat actccagcac atcgtggagg
     3301 taaccttgcc caattagtat gctcgaatag ttttaagggc atcgccaaaa caacagcgca
     3361 tgggcttcta aaaaatgaat tgactttact tggctctttt ttaattgaac ttgccaaaga
     3421 agctcaagtc cctgctggag aatctttaac ggtcaatcgc gaaattttta gtgctctagt
     3481 cgaaaagaaa atttctgaat gccctgcgat tacgttacat cgagaagagg tgacttcttt
     3541 agaagatgac ctccccactc ttgtcgctgc agggccttta gctagcgagg ctctatctaa
     3601 ttccattttt aacgccctag gcagtgaacg tcttcatttt tttgatgcga ttgctcctgt
     3661 cgtagaagcc gatagcattg attttaacca tgctttttac atgaaccgct gggaaaaagg
     3721 ggaaaccgcg gattttatca attgccctct agacgtagaa acttataacg cgtttgtaga
     3781 aaaacttcgt gaagctgatt ctgtggaacc aaggcctttt gaaaaaaatg aactctttga
     3841 agggtgttta cctatcgaag aaatggctag acgcgggaca gaaactttaa gatatggccc
     3901 tatgcgccct attggattag ggtttggcaa tgaaggcaag aagtggcacg cggtgattca
     3961 acttagagct gaaaatgcac aaaaaactct ttttaatatg gttgggtttc agacgcgcct
     4021 aaaatggaac acgcaaaaag aacttttcgc aatggttcct gctcttaaaa atgctcgttt
     4081 tgctcgttta ggttgtatgc atcgcaatac atttatagaa agccctaaat tattagaacc
     4141 aacacttcaa ctaaaaagag ggcttcctgg ttgtgaaaaa ctccctccta tttggtttgc
     4201 tggccaaatt actggtgcag aaggttatac cgaagccgta gccactggct ggtatgccgc
     4261 ttggaatatg gcttgttctt taaaacatgg tgtgcctcaa ttactcccaa aagaaagttg
     4321 tattcgttct ttagtcgatc aattagtaag cccaaatcct gattttcaac caatgaattt
     4381 caattttggt ttactgcctc atttaattga gatgaaaaag aagaataaaa aagagattct
     4441 cggaaatcaa gcagaagaag ctgtcataga gtggataaga aaaaaaccgt attagataac
     4501 taatacggct ttaaataccc ccaccgcgag tcgaacgcag gtatcaccct taggaggggc
     4561 gcgctctatc catctgagct atgagggcat ttgaaaaacg aagatagaat taattgggcc
     4621 ctttttcaag caaagtaaaa aagcctatta acgcattttt ccagagcgaa gcacatccat
     4681 catattaaat ggtcttgctt tgccttcttt taatttttca gctaagaatg caacggcatc
     4741 gacggtgcct tcggcataga cgtgacggcc acatacatta tgttggaatt caaaatgcac
     4801 agagccatcg gcactatcta aagaatacgt gtgaaaagcg tgaccactta aagcatcttg
     4861 tggaacatgc attcgctcca attgctgttt ttcatcgcgg actaattcaa tatctttttc
     4921 tgaaaagtca aagcccattt tttgaaaaga agcaactact gcttttgcag taccgctcgt
     4981 atctgctttt gtcttttgat ggctttccac aacgcttaac ttataatcac taaatgccgt
     5041 tggaaattca tttgctaaga attcgatcat cgtttgaaaa gcgacaattt gctttgccat
     5101 attaggagca atgacgcttg gatgattatt ttcttctact aatttttgaa gtgtttctcg
     5161 atcaccgcca gtagtgccca tgacaaaagg gatcccgtgc tttacataaa aagctgcatt
     5221 atcgtttact gctgtaggat gcgtataatc tatacaaatc atatcaggat aacgtttcat
     5281 tacatcagcg atgatgtttt cacgtgtact tggttttagt tcgactttta cgcctgcgac
     5341 catcttttct ttttcttcaa cgatttcacc cgttaaagaa taaggcacta gttttaaacc
     5401 gcgagcaaca caagtctcag ccaccatgca tcccatgttt cctggaatac catttaccat
     5461 tacatatact gacataaaaa ccgtcctatt agcaaaatcc aattctaatt aaggattctt
     5521 tgtaaaacga atataggctt gaagagtatc gcaaaatact tcatcaggag aattatctcc
     5581 gacgatacgt gtcacaatct tttcgtcata tttagaaaga acagcagctg ttttttcacg
     5641 atattcgact aagcgattct taataatcga ttcatcggca tcatctttac ggccttcgat
     5701 ttttgctcta tttaagagac gagctacgat tgtggcatca tctttaattt cg
//
LOCUS       DAOOBB010000129         5736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_16184_length_5736_cov_5.318078, whole genome shotgun sequence.
ACCESSION   DAOOBB010000129 DAOOBB010000000
VERSION     DAOOBB010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 5736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5736
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16184_length_5736_cov_5.318078"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="PLT31_06600"
     CDS             complement(<1..893)
                     /locus_tag="PLT31_06600"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HPW94844.1"
                     /translation="MAKASKKTAKNETKKVVLAYSGGLDTSVIIPWLKENYDCEVIAF
                     AADLGQGDCDAEALKKKAIATGASKCYVLDLRKEFLEDYVWPTVRAGAKYELTYLLGT
                     SFARPLIAKYQVKIAEAEGAYAVSHGATGKGNDQVRFELTYAALNPSLEIIAPWKDPK
                     WNIHSREDAIDYAVARKIPLNGISKKKIYSEDGNLWHLSHEGGILEEPDKEHKYDMLK
                     LSKTFEKAPAKPGYVTIGFEKGTPVSIDGKKMDAVSLLTYLNKIAGEHACGLLDIVEN
                     RLVGLKSRGVYETPGGSLLYKA"
     gene            1023..2024
                     /locus_tag="PLT31_06605"
     CDS             1023..2024
                     /locus_tag="PLT31_06605"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPW94845.1"
                     /translation="MKISDKTIGYVSIICLILIFGIVTLGMYQAHESKTIEIEVDFLE
                     LGSIQPQDAVTIDGYKVGQIGSIKWLGNKARLQLKFDEPIVLREGTRIINSNYALMGQ
                     RRIEIIRSKTGEVVPKDYVFEGEFQPGIAEALRLIENLQTQMEIIRDIALLLIHGDSV
                     TPSVPKMFETKMEQMDHLISSLEKLTEQVNSQVNGLLVQTTDASKQIIETADKAQGII
                     DTIEEVTSSKIEEANKALASISKTSSEANDFILSLENNPSIHNLLNSTEKIEKINELI
                     EKVNLLSKAIQKEGVALYNEKGERVKLFPFKNMNIIGKTAREKAKDREKEDLIQKNQ"
     gene            2074..2146
                     /locus_tag="PLT31_06610"
     tRNA            2074..2146
                     /locus_tag="PLT31_06610"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2107..2109,aa:Asn,seq:gtt)
     gene            2691..2763
                     /locus_tag="PLT31_06615"
     tRNA            2691..2763
                     /locus_tag="PLT31_06615"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2724..2726,aa:Asn,seq:gtt)
     gene            complement(2796..3116)
                     /locus_tag="PLT31_06620"
     CDS             complement(2796..3116)
                     /locus_tag="PLT31_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547003.1"
                     /note="involved in start site selection during the
                     initiation of translation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HPW94846.1"
                     /translation="MDERSTIVYSTETGRIKEKKEPERAPEGDGIVRISLKRLGGGKA
                     VSIVTGLALETDDLKELCRLLKQKCGVGGTVKDFTIEIQGDKRTIIQTELEKKGFRVK
                     LAGG"
     gene            complement(3119..4765)
                     /locus_tag="PLT31_06625"
     CDS             complement(3119..4765)
                     /locus_tag="PLT31_06625"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HPW94847.1"
                     /translation="MLKKHLGLLVLALCSFSTAQNIMSNGNMEYGDGGWYLWNKPEGP
                     AKVEFKIAEPKIGVDGSQGAVVVIKEPPHIWWGLQIQPPKFLTDSAYYTLSFKAKGDI
                     LVTAIVQGGPPDYRQKESSVIQLTKDWKTYTMTFLADQKGYGLNNVSFQIGHQKGTLW
                     LDDVEVRLAEGAADTTWYLSAEARIDSIRKVDFSVPAKPGEKVSVELLRHEFPFGTAL
                     ALYDSKDSVEKWYRSTAAKYFWHGVTENQFKWPEYEPKKGKLRKDELNEYFKFAKENG
                     WDLRGHALVWAIQGYNYDKHFSNKGSCKEIAKNIKQRIDRDLKEYKGKFVDYDVWNEP
                     FHERFLFNKCGWDLMDSAFVWAHRADPTAVLYINEYNVVSAGETDFYYDIIKRMLDKK
                     IPVQGIGVQCHYQGRPVSPGLFKERIDKLATFGLPIKITEFDIGTFDQGLHVSEQKQA
                     EEFAKFIRSAYSHPAVSGILLWGFWDNRHWLKNGGIIASDGRAKPAADSVYNLWHKTW
                     TTRSSAVADSSGKANFRGFPGKYQVTIGKEKKTLEVKSRK"
     gene            complement(4773..>5736)
                     /locus_tag="PLT31_06630"
     CDS             complement(4773..>5736)
                     /locus_tag="PLT31_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94848.1"
                     /translation="IVLEAAMHLHLTRDALEVMSKAAISAASLLLVTSGVITGLLHLV
                     SGETFHSCLVNAIPFAVISSAMAIPSAQNLSPEKKEFVIYESTFSDILGILFFTLASN
                     NLGGVDDWVLGISKSVLITILFSAIFTVGLVWLVTKLRNHVKFFLILFLLLLVYSVAK
                     LFHLPALLLVFLFGLALNNTFLLNRWKWLSEKIDLEKVQEDLKYMQVSATESTFLIRT
                     FFFVIFGYSLQWALILKLDVILLSLAVAAIIFGLRFGFFKLLAKKHLFPEFLIAPRGL
                     ITVLLFYSIPQTMQIIPVNLGSVFLVVVITAITMAAGTWKKRQS"
BASE COUNT         1801 a         1236 c          999 g         1700 t
ORIGIN      
        1 gctttgtata aaagcgagcc acctggagtt tcataaacac cacgactctt taagccaact
       61 aaacggtttt caacgatatc taaaaggcca caagcgtgtt cacctgcaat cttattcaaa
      121 taagtgagta aagatacggc atccatcttt ttgccatcaa tagaaacagg agtccctttt
      181 tcaaagccaa tagtcacata accaggtttt gctggagctt tttcaaaagt cttggaaagc
      241 ttaagcatgt catatttatg ttctttatct ggttcttcta aaatgccacc ttcgtgagaa
      301 aggtgccaaa ggttaccatc ttcagaatag attttctttt tgctaatgcc atttaatgga
      361 atcttacgag ccacagcata atcaatcgca tcttcgcggc tatggatatt ccattttggg
      421 tctttccatg gagcaataat ctctaatgaa ggatttaatg cggcataagt taattcgaaa
      481 cgaacttggt cgttaccttt acctgtagca ccatgagata cagcgtaagc accttcggct
      541 tctgcaattt taacttggta ttttgcaatc aaaggacgag caaaagaagt acctaataaa
      601 taggtcaatt catattttgc gccagcacga acggtaggcc aaacgtaatc ttctaaaaat
      661 tctttgcgta gatcaaggac gtagcacttt gaagcgcctg tagcaatggc ttttttcttt
      721 aaagcttctg catcacaatc gccttgccct aaatcggctg caaaagcaat cacttcacaa
      781 tcgtaatttt ctttaagcca aggaataata acggaagtat ctaacccgcc actataagcg
      841 agaaccactt tttttgtttc atttttagct gtttttttgg aagcttttgc cataaataat
      901 cctttgatga atttctatgc ttgaaaatat agttattttc tacatgtaat gacagtctgt
      961 agacgctctg tttttaccat aagcaatcta ttcccgcttg acatttgtta tattaatctt
     1021 tcatgaagat ttccgacaaa acgattggct acgtctctat catctgcctt attttaatat
     1081 ttggcatagt gactcttggg atgtatcagg ctcacgaaag caaaaccatt gaaatagagg
     1141 tcgactttct tgaattaggc tctatccagc ctcaagacgc tgtgactatc gatggctata
     1201 aagtgggtca aattgggtct atcaaatggc ttggaaacaa agcaagactt cagttaaaat
     1261 ttgacgagcc tattgttctt cgcgaaggca ctcgtattat caactccaat tacgctctga
     1321 tgggtcaacg ccgtattgaa attatcaggt ctaaaacagg cgaagtagtc cctaaagact
     1381 atgtctttga aggcgaattt caaccaggca tcgctgaagc tttaaggtta atagaaaatc
     1441 ttcagacgca aatggaaatt atccgagaca tcgctcttct gctcatccac ggcgactccg
     1501 taacaccgtc tgttccaaaa atgtttgaaa cgaaaatgga acaaatggac catctcattt
     1561 cttctcttga aaaactaacc gaacaagtga attcacaggt caatggtctt cttgttcaaa
     1621 cgaccgatgc ctctaagcaa atcatagaga cagcagacaa ggcccagggc atcatagaca
     1681 ccatcgaaga agtcacttcc tcaaaaattg aagaagcaaa taaagcactt gcctccattt
     1741 caaaaacatc ttcggaggct aatgatttta ttttgagcct agaaaacaat ccttcaatcc
     1801 ataatctttt aaattcaact gaaaaaattg aaaaaattaa cgagttgatc gaaaaagtga
     1861 atcttttgtc taaagcaatc caaaaagaag gcgtcgcctt atacaatgaa aaaggggaac
     1921 gagttaaatt atttcccttt aaaaacatga atattattgg aaaaacggct agagaaaaag
     1981 caaaagatcg agaaaaagag gatttaatac aaaaaaatca ataaaatcct tgacagtgat
     2041 ttatagctta ctatatttgt ccacaacaat tgatgcctca tagctcagtt ggtagagcgt
     2101 tcggctgtta accgaattgt ccctggttcg agtccaggtg aggcagcgaa aaaatcccga
     2161 tgtaaatcgg gatttttttt atgccacgtt tgcgcgatat ttgtgccaca tttgcgccat
     2221 attctcgcca ttttcgcacc acggtttgca cgatattcgt tccatattct cgcatttttc
     2281 gcagcacgtt cacgccattt tcgcgcggca ttcgtgccaa ggtttgcacg atattcgcgc
     2341 caagttcgcg ccatattctc gtcattttcg caccacggtt tgcacgatat tcgttccata
     2401 ttctcgcatt tttcgcagca cgttcacgcc attttcgcgc ggcattcgtg ccacgttctt
     2461 tataagtact taagcgaaaa actttttcct taaaacccat aaatcgaaag cccaaatatc
     2521 ttctttaaaa tgaatgatta gaagagaacg gataaaatga tatttcggtg cattatcgtt
     2581 ttgtaacaca cgcctttaaa gcgacagaaa taccccttaa tcgagtcttt ttttgaatag
     2641 attcgtcttt acattttata aaagcggatg tatatttaca ccataattga tgcctcatag
     2701 ctcagttggt agagcgttcg gctgttaacc gaattgtccc tggttcgagt ccaggtgagg
     2761 cagcgaaaaa atcccgatgt aaatcgggat tttttttatc ctccagcaag cttcacacga
     2821 aatcctttct tttctaactc cgtctgaatt atagtgcgtt tatcgccttg aatctcgata
     2881 gtgaaatctt ttactgttcc accaacgcca catttctgct tgagtaagcg gcataattct
     2941 ttaagatcat ctgtttctaa ggcaagacca gtaacgatac tcacggcctt gcctccacct
     3001 aaacgtttta atgagattcg aacaatccca tctccttctg gagcacgttc aggttctttc
     3061 ttttctttga ttctgcccgt ttctgtagaa tagactattg tagaacgttc atccatactt
     3121 atttccgaga cttcacttct aaggttttct tttctttccc gattgtcact tgatacttgc
     3181 ctgggaagcc tctaaaatta gccttgccag aagaatccgc aacagcagaa gaacgagtcg
     3241 tccatgtttt atgccacaaa ttataaacag aatcagcagc aggtttcgca cggccatccg
     3301 aagcaataat gcctccattt tttagccaat gacgattatc ccagaaaccc catagcaaaa
     3361 taccactcac tgctggatgg ctataggcac tgcgaataaa cttcgcaaat tcttcagctt
     3421 gcttttgttc agaaacgtgt aagccttgat caaaggtacc aatatcaaat tctgtgattt
     3481 taattggtaa accaaaagta gcaagcttat caattctttc cttgaataac cctggagata
     3541 ctggacggcc ttgataatgg cattgcacac cgattccttg aactgggatt ttcttatcta
     3601 acattctttt aataatgtca taataaaagt cagtttcgcc agcagaaact acgttatact
     3661 cattaatgta aagaacggcc gttggatctg ctcggtgcgc ccaaacaaaa gcactatcca
     3721 tcaaatccca accacactta ttgaaaagaa aacgttcgtg gaatggttca ttccagacat
     3781 cataatccac aaattttcct ttatattctt ttaagtcacg atcaattctt tgcttaatgt
     3841 ttttggcgat ttctttacaa cttcccttat tagaaaagtg cttgtcataa ttgtaacctt
     3901 gaatagccca cactaaagca tggccacgta gatcccaacc gttttcttta gcaaacttaa
     3961 agtactcgtt taattcgtct ttgcgtaact ttcctttctt aggttcgtat tctggccact
     4021 taaactggtt ctctgtaaca ccgtgccaga aatatttagc cgctgtacta cgataccatt
     4081 tttcaacgct gtctttagaa tcataaagag caagcgctgt accaaaaggg aattcatgac
     4141 gtagcaattc tacagagact ttttctcctg gctttgcagg tacagaaaaa tccactttac
     4201 gaatactatc aattctagct tctgcagaaa gataccaagt tgtatcagca gcaccttccg
     4261 ctaatcttac ttcgacatcg tcaagccaga gagttccctt ttgatgacca atctggaagc
     4321 tcacgttatt taagccatag cctttttgat ctgcaaggaa agtcatcgta tacgttttcc
     4381 aatctttagt caattgaatc acagaacttt ctttctgacg ataatcggga ggcccgcctt
     4441 gaacgatggc agtgactaag atatcaccct ttgccttaaa ggaaagagta tagtaagctg
     4501 aatcggtaag aaattttgga ggttgaattt gtaagcccca ccaaatatga ggaggttctt
     4561 taatgacgac tactgcaccc tgagaaccat caacacctat tttaggctct gcaattttga
     4621 attccacttt agctggtcct tctggtttat tccagagata ccaaccccca tcgccatatt
     4681 ccatatttcc attgctcatg atattttgag ccgtagaaaa agaacataag gctaaaacta
     4741 atagacctag gtgttttttt aacatagtga cctcagcttt gacgtttttt ccaagtacca
     4801 gcagccatgg taatagcagt aatcaccacg actaaaaata cagagcccaa attcactggt
     4861 atgatttgca tcgtttgagg aattgaatag aataaaagaa ccgtaatcaa tcctcttgga
     4921 gcaattaaaa attcagggaa tagatgcttt tttgcaagta gcttgaaaaa accaaaccga
     4981 agcccaaaaa taatagcagc cactgctaat gataataaaa tgacatctaa ctttaatatc
     5041 aaagcccact gtaaagagta tccaaaaatc acaaaaaaga acgtgcgtat aagaaatgtg
     5101 gattccgtag cagaaacctg catgtattta agatcttctt gtactttttc taaatcaatt
     5161 ttttcactta accatttcca gcgatttaat aagaaggtat tgtttaaggc aaggccaaat
     5221 aagaacacta acaataaagc gggtaaatgg aataacttcg cgacactata cactaaaaga
     5281 agcaagaata aaatcaaaaa gaacttcacg tgattacgaa gttttgtgac aagccaaacc
     5341 aagcccacag taaaaattgc agaaaaaaga atcgtaatta aaacactctt agaaatgcca
     5401 agaacccaat catctacccc gcccaaatta tttgacgcta gagtaaaaaa tagaatgcct
     5461 aaaatatcag aaaaagtcga ttcataaatc acaaactctt ttttttctgg tgacaaattt
     5521 tgagcgctag gaattgccat cgcacttgaa atcacagcaa aaggaattgc attgacaaga
     5581 caagaatgaa aagtttctcc actcaccaag tgcagtaaac cagtgataac gcccgacgtc
     5641 actaataaaa gagaggctgc cgaaatggcc gctttagaca ttacttccaa ggcatctctt
     5701 gttaaatgca aatgcatggc tgcttctaac acaatt
//
LOCUS       DAOOBB010000130         5735 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_16188_length_5735_cov_5.868310, whole genome shotgun sequence.
ACCESSION   DAOOBB010000130 DAOOBB010000000
VERSION     DAOOBB010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16188_length_5735_cov_5.868310"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1947)
                     /gene="metE"
                     /locus_tag="PLT31_06635"
     CDS             complement(<1..1947)
                     /gene="metE"
                     /locus_tag="PLT31_06635"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF003556.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HPW94849.1"
                     /translation="MTNKTWITGFSRIGENRELKKALELFWKGKSSKEELESVAKELR
                     KKHWLEQKTKGVEYISSNDFSFYDHMLDTAVMLNAIPSRFQKIKDPTTLYFSMARGSK
                     DAQAMEMTKWFNTNYHYIVPELSPSISFKLNAEKIIKEYNEAFELGIKTKINLIGPLT
                     FLSLCKTENGEDPFTYLDSVLCIYKELLAKMAKLQANTVFQFEEPIFVRNPTLKLLSK
                     IAFTYQSLCEVNRKNKIAVVTYFEHATEALKELSKTDVWGIGLDFVYGSYNMSALSEI
                     RNKKIIVGLVDGRNVWANDYEESLDLLEKISAEIPKENMIVSTAASLLHCPYSLKGEP
                     HKDLYQWFSFGVEKIVEVVFLSELFFSENRSEKNEERLNKNKQIFIEKRRSRFITDDN
                     VQKRMGSFITKERSMDFNARYKIQREKFNLPLLPTTTIGSFPQTAELRQLRRDYKNNT
                     ISKHDYETQIKAYIDECVALQEEIGLDVLVHGEPERNDMVEYFGELMQGFLFTQKGWV
                     QSYGSRCVKPPIIFGDVSRPYPMTVPWISYAQSKTSKKMKGMLTGPVTIINWSFARND
                     ISKAEVAKQIAMALSDEIVDLQKAGISIIQVDEAAFKEGYPLRKEKVQEYENWAVSSF
                     KMAVSTARPETQIHTHMCYSDFNDI"
     regulatory      complement(1948..2086)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01482"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="The adenosylcobalamin (AdoCbl) riboswitch is found
                     in the 5'-untranslated regions of vitamin B12 (Cobalamin)
                     related genes in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF01482"
     gene            complement(2346..3599)
                     /locus_tag="PLT31_06640"
     CDS             complement(2346..3599)
                     /locus_tag="PLT31_06640"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HPW94850.1"
                     /translation="MNYIEKHLEYFFTSFGLSENIAHYVRLLTLLVLLSLLSFFFFWV
                     TKKWVIPLFYKFFKKTSFTWDDILVEKNAFRKLAHITPALIIKIGAPILFRDFPSSLS
                     IVNKLTDAYLIFASVLIVVALLEVVESLFSKMPVFSDKPIGSYFQLGRIILYLISAIL
                     ILSVLLGKSPVYFLSVFGAMSAVVLLIFKDTILGFVSSIQMSANDMVRVGDWIEMPKF
                     NADGDVIAINLNTVKVRNWDKTVTTIPTYFFTSDSFKNWRGMQESGGRRIKRSLNINV
                     KTVKFVDEKAREFYNKIFLLKDYVQLRQEEIEQFNSLYDFDPTVPINGRRMTNLGVFR
                     HYVENYLKNHPKIHQGMTLLVRQLPLEDKGIPLEVYCFTKTTAWNEYEDIQADVFDHL
                     LASASFFDLEVFQQVSGSDFSKNHL"
     gene            complement(3646..4167)
                     /locus_tag="PLT31_06645"
     CDS             complement(3646..4167)
                     /locus_tag="PLT31_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94851.1"
                     /translation="MPQKDRISIKFKEKLERELSADESVLWADEPAPVFAKKIFFCLM
                     FLLFFILWLAIDSFNAWTASLFAVIIVAVIILGSKVRFKKTLYVITNKRAIIIQGAKT
                     YTIRSYPPHLLQFIYLTEKQMGIGDLIFEENISVTPSRSRKVQQFGFLNVRKVKLVEQ
                     MLRTLAGQESSKK"
     gene            4441..>5735
                     /locus_tag="PLT31_06650"
     CDS             4441..>5735
                     /locus_tag="PLT31_06650"
                     /inference="COORDINATES: protein motif:HMM:NF013438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HPW94852.1"
                     /translation="MQKNYFFSLSFSLLLALALPSYAVLEIPNTQPKVGAEVWKPILD
                     KTWEGIKKRNIQPYGTGLIHRPKSETPGDAVSEGVGYGMILALYANDQKTFNQIWDAS
                     EKYMFNNNAKIYDWRVNQSGTLIGHGPATDADEDIALMLIFADKLVQKGLWESYTSPK
                     YNVTYAQRAQDILNAIWSIMIQDGRFVKPGDWGGSGLLNAGYFSPASYRIFKDFDNNK
                     DSHHWDNVIEQCYQTLKANNTLYEKGMIPDWSDAYGNFLSNGPGYNAYLKGKSFFKDA
                     IRILWRISLDAIWFDEPKAKEFLTNSLNFINSKGGASASNYYQLEGSLLGELLPKEDI
                     WKEFNDQNNEETWRYRREHSHLTIGMWSTAATAVGSDEDKISFTNEMAKFYEYDEDAD
                     FFGLILDTENLEDTLHNEMYFDQFLAWFGVSLMSGTFCNV"
BASE COUNT         1914 a         1096 c          879 g         1846 t
ORIGIN      
        1 aatatcatta aaatcactat aacacatatg agtatgaatt tgtgtttctg gacgagcggt
       61 gctaacagcc attttaaaac tacttacggc ccagttttca tattcttgaa ctttttcttt
      121 gcgtaaagga tacccttcct taaaagccgc ttcatccacc tgaataattg aaatccccgc
      181 tttctgtaag tctacgattt catcactcaa agccattgca atttgctttg ccacttctgc
      241 ttttgaaata tcatttcttg caaaagacca attaataatc gtgacagggc ccgtcaacat
      301 ccccttcatc tttttagacg ttttactttg tgcataagaa atccaaggca cagtcattgg
      361 ataaggtctg ctcacgtcgc caaaaataat tggtggtttt acacaacgac tgccataact
      421 ttgtacccat cctttttggg taaataaaaa gccctgcatt aactcaccaa aatactccac
      481 catgtcgttt ctttcaggtt ctccatgaac aagaacatcc aaaccaatct cttcttgcaa
      541 agcaacacac tcatcaatgt acgctttgat ttgtgtttca taatcatgtt ttgaaatagt
      601 attgttttta taatctcgtc gtaattgcct taactctgcc gtttgaggaa aacttccaat
      661 tgttgttgta ggtaataatg gcaaattgaa tttttctctt tgaattttat aacgtgcatt
      721 aaaatccata gatctttctt ttgtaataaa agagcccatt cttttttgaa cattatcatc
      781 tgtaataaaa cgagatcgtc ttttctctat aaaaatctgt ttgtttttat tcaagcgttc
      841 ttcatttttt tcacttctat tttctgagaa aaaaagctca ctaagaaaaa caacttctac
      901 aattttttct actccaaaag aaaaccactg gtataaatct ttatggggtt cccctttcaa
      961 agaatatgga cagtgcaaaa gagacgccgc tgtagaaaca atcatatttt ctttaggtat
     1021 ctctgcactt attttttcaa gtaaatctaa agattcttca taatcatttg cccaaacatt
     1081 tcttccatct acaagcccaa cgattatttt tttatttcta atttctgata gagcgctcat
     1141 attataagag ccataaacaa aatcaagccc aatgccccaa acatctgttt ttgataactc
     1201 ttttagcgct tctgtggcat gttcaaaata agtcaccaca gctattttat ttttacgatt
     1261 cacttcacat agtgattgat aagtaaaagc aatttttgaa agtagcttta aggttggatt
     1321 tcttacaaaa ataggctctt caaattgaaa caccgtatta gcttgaagct ttgccatttt
     1381 agcaagtaat tctttatata tacaaagcac cgaatctaaa taggtaaaag gatcttctcc
     1441 gttttcggtc ttacatagcg ataaaaacgt aagtgggcct attaaattaa tctttgtttt
     1501 gatccctaat tcaaaagctt cgttatactc ttttataatc ttttctgcat ttaatttaaa
     1561 agatatagaa gggcttaatt ctggaacaat gtaatggtaa ttcgtattaa accattttgt
     1621 catttccatt gcttgagcgt cttttgatcc tctagccatt gagaaataaa gagttgtagg
     1681 gtcttttatc ttttgaaatc gagatggaat ggcgttgagc atcaccgctg tatcaagcat
     1741 gtgatcataa aagctaaaat cattggagct tatatactcc accccttttg ttttctgttc
     1801 tagccaatgt ttttttctta attcttttgc aacagactct aattcttctt tagaagattt
     1861 ccctttccaa aaaagttcaa gcgctttctt taattcacga ttttcaccaa tacgagaaaa
     1921 tcctgtaatc caagttttat ttgtcatagc aaactccttt tgttttcgcg acaaagtacg
     1981 ttttgttact atcgtggtat tcggacttag aacatttagt tccacaccgt agcgcgactg
     2041 tgctcgattc acacgagctt ccccacatgg ataataacga actaaattta tgaaaaaaat
     2101 gggatttttg tgtctataaa atgcatttat gtgcatttat gcattaaatt atcgctttac
     2161 ttaataaaat ttgagtgaaa cccagtgact gggcaaagcc aaccagcacg aagagttgca
     2221 accaatgttt tgaaaatgga ttgacgtgct aaataagtgt tataaaaata cttgcgtttt
     2281 catgactgta ttgcgccatc aaaaaaagaa taatcatttt taaggagact aatattttca
     2341 aagaatcaca aatgattttt tgaaaaatca ctcccgctta cttgctgaaa cacttctaaa
     2401 tcaaagaaag atgccgaggc aagcaaatga tcaaaaacat ccgcttgaat atcttcatat
     2461 tcattccatg ctgttgtttt cgtaaaacaa tatacttcta aaggaatccc tttatcttcg
     2521 agaggaagtt gccgcactaa tagagtcatc ccttgatgaa ttttcggatg atttttcaga
     2581 tagttctcca cataatgacg gaacactccc agattagtca ttctccgacc attaatcggt
     2641 actgtcggat caaaatcata caaactatta aactgttcta tttcttcttg tcgtaactgg
     2701 acataatctt tcaatagaaa tattttatta taaaattctc gggctttttc atccacaaac
     2761 ttcacggtct tcacattaat atttagcgaa cgcttaatac gacgtccacc actctcttgc
     2821 attccgcgcc aattcttaaa gctatcagaa gtgaaaaaat acgttgggat tgtcgtcacc
     2881 gttttatccc aattccgaac ttttactgta tttaaattaa tcgcaatgac gtctccatcg
     2941 gcattaaatt tcggcatctc aatccaatca cccacacgca ccatgtcatt agcagacatc
     3001 tgaatgctag aaacaaagcc caaaattgta tctttgaaaa tcaaaagaac aaccgcagac
     3061 atggctccaa aaacactcag aaaataaacg ggcgatttac caagcaacac tgataaaatt
     3121 aaaattgcag aaataagata aagaataatt cgccctaact gaaaataact ccctatcggt
     3181 ttatcactaa atacaggcat tttagaaaat aacgattcaa ccacttctaa taaagcaacg
     3241 acaatcaaaa ccgatgcaaa aatcaaataa gcatccgtta atttatttac gattgataaa
     3301 gaagaaggga aatctcgaaa taaaatagga gctcctattt ttataataag agcgggggta
     3361 atatgagcta atttacgaaa tgcatttttt tcaacaagaa tgtcatccca agtaaaagag
     3421 gttttcttaa aaaacttata aaataaagga ataacccatt tttttgttac ccaaaaaaag
     3481 aaaaaggaca acaaggataa taacactagt aaagttaata atcgaacata atgggctatg
     3541 ttttcagaaa ggccaaaaga cgtaaaaaaa tattctaaat gtttttctat ataattcatt
     3601 tatttcaaga taaaaaactt tatcctaatc atcaaacaaa ccaatttatt ttttagaaga
     3661 ttcttgccct gcaagagtac gaagcatttg ttctaccagt tttacttttc tcacatttaa
     3721 aaaaccaaac tgttgtactt ttcgagaacg acttggtgtc acagaaatat tttcttcaaa
     3781 aattagatcc cctataccca tttgtttttc cgttaaataa ataaactgaa gtaaatgtgg
     3841 tgggtaactc ctaatcgtat atgttttagc tccttgaata attatcgctc ttttattggt
     3901 tataacataa agcgttttct taaaacgtac ttttgagccc aaaataataa cagcaacaat
     3961 tattactgca aataaggaag ctgtccaagc attaaatgaa tctatagcca accataaaat
     4021 aaagaataga agaaacatta agcaaaaaaa tatttttttt gcgaaaacag gagcgggttc
     4081 atccgcccac aaaacagatt catcagcaga aagctctcgc tccaattttt ctttaaattt
     4141 tatagaaata cggtcttttt ggggcataag agaactcctt tactctcaac gctagaacac
     4201 aaacattttc gtccgttctt cccaaaacac gactcaacaa atcacatacc atgtgattat
     4261 cttaaaataa caagaaatca acgtaaaaaa agtaaaaaaa ggttgcaaag tacgctttta
     4321 taataaaaaa gacgtgatga atctcgcctg tttactttag aaaacaaaca agcaaaaaat
     4381 aagaccctga atatacttta atagtttatt tttagagttc acctttatat tccaattatt
     4441 atgcaaaaaa attatttttt ctctttatct ttttcccttt tattagcgct agcattgcct
     4501 tcctacgctg ttttagaaat ccctaacact caacctaaag ttggtgcaga agtctggaaa
     4561 cccattttag ataaaacatg ggaaggtatc aagaaaagaa acattcaacc ttatggtact
     4621 ggactaattc accgtccaaa aagtgaaact ccgggtgatg ctgtttccga aggtgttggc
     4681 tatggaatga ttttagctct ttatgcgaat gatcaaaaga cctttaatca aatatgggat
     4741 gccagtgaaa agtatatgtt caacaataat gctaaaatct acgattggcg tgtcaaccaa
     4801 tctggtaccc taattggaca tgggcccgcc accgatgcag atgaagatat cgcccttatg
     4861 ctcatttttg ccgataaact ggttcaaaaa ggcctatggg aaagctatac tagcccaaaa
     4921 tacaatgtaa cctatgctca aagagctcaa gatattctaa atgccatttg gtctatcatg
     4981 attcaagatg gtcgatttgt caaacctgga gactggggtg gttcaggttt attaaatgca
     5041 ggttattttt ctccagcctc ttaccgcatc tttaaggatt ttgataacaa taaagattca
     5101 caccattggg ataatgtgat tgagcaatgc tatcaaaccc ttaaagccaa caacactctt
     5161 tatgaaaagg gcatgatccc tgattggagt gatgcctatg gaaacttctt aagtaatggc
     5221 cctggttaca acgcttactt aaaaggcaaa tcttttttta aagacgccat tcgcatttta
     5281 tggcgtattt ctttagatgc catttggttt gacgaaccca aagctaaaga gtttttgact
     5341 aattccttga attttattaa tagtaaggga ggagcctcgg cttcgaacta ctaccaattg
     5401 gagggctcgt tattaggcga actactccct aaagaagata tatggaaaga attcaacgat
     5461 caaaataacg aagaaacttg gagatatcgt agagaacaca gtcacctaac cataggaatg
     5521 tggtctacag ccgctactgc agttggctct gatgaagata aaattagttt tacaaatgaa
     5581 atggccaaat tttatgaata cgatgaagat gctgatttct ttggcttaat tctagatact
     5641 gaaaatttag aagatactct ccataacgaa atgtattttg accaattcct tgcttggttt
     5701 ggcgtttctt taatgagcgg aacgttctgc aatgt
//
LOCUS       DAOOBB010000131         5729 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_16214_length_5729_cov_5.486782, whole genome shotgun sequence.
ACCESSION   DAOOBB010000131 DAOOBB010000000
VERSION     DAOOBB010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 5729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5729
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16214_length_5729_cov_5.486782"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..3694)
                     /gene="smc"
                     /locus_tag="PLT31_06655"
     CDS             complement(152..3694)
                     /gene="smc"
                     /locus_tag="PLT31_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HPW94853.1"
                     /translation="MHITKLKIFGFKSFAQRTEITFPGNGLTAVVGPNGCGKSNIVDA
                     IRWVLGEQKAAILRMGKMQDVIFSGTEERAAMSLAEVSLVINNDDGRLPLPYAEIMVT
                     RRAHRNGTSEYLINNQECRLKDIQNLFFDSGLGTGTYSQMNERMINAVLSDKAEDRRT
                     LFEEAAGVSKYKQQRKETLRQLERVQVDMARVEDNLNSTRRSVRQYEKQAEKTREWME
                     LRRRLKELDLSLSLDKYEDNKKSVSILDTSRKRVEHDLESSKTRLTVLKTQIDEKRLA
                     ISDDENSLRELEQLVQKEELSLNNMNNELMRFRETLVTLEESSNKYRKEIEESREKQT
                     TLRTEKELLEEKTAHLGSDDEMDEQVRLLEQEREVLQVLRNAYDDSRKSSQELAEKRI
                     QVLNKANSLRSRWQRTDTEKDLLLKDIENRDKEFSDIEEQKKEAQGNLEAMLEGIAEA
                     ERELQMLEERQCVQAEKVESSKEEIQKLEKRLQNYKNEEMRLQSRFDVLSSMAISEGE
                     GAKWLLENKSQEIAGLLGQKIEADPRYVSQIEFALGEALDAVLLQDANLADSLLNALE
                     QASVGSVLLASFAGESSFESSSIEGDRVIGPAHRFVSAAPEIRPLLERWLSKWILVED
                     FETARRLAKENASKDLWFLSPGRAVHTAGFIRARGGSSESLGILSRRTEIDELEKKLD
                     QIKSELQSIETSYNRSQDLLDEDQTVFNSILEDIREKQAQIQSGNAAKLIHQHTIQNM
                     DRQLERIENERSRAKSRISAMESERSEDSELKEAETLATQVEDEYQKTLAKAQEQELM
                     YRNKESDIREIEMRLGEKRSLISASVSRLNSIGEQLSMLEELILTREQEIEGIEIKAT
                     QVTQESETLASQIEEKHEALRVLERRRDEARERYNVVSGDLEEWRGEERSINDSLVER
                     ISELKDIERRLESLSANIERLRERIFSEWEVDLDNTDDVVRVEYEVSSADREIREIRG
                     KIKALGPVNQGIMDDFEEEKHRLEEVERQFDDLDRARASLERTIHKLDIIARDRFMDT
                     FHKIQKNFQEVFSRLMHDGGTKLTLEEGVDPLEAEIEVNARPTGKKMRGVRALSGGER
                     ALTAVSLLFAIYMEKPSPYCVLDEVDGPLDDANIGRFMELLRHFSKQTQFILVTHNKR
                     TMASADMLYGVTQEIKGISRIASVKLDDAVGFAV"
     gene            complement(3725..4102)
                     /locus_tag="PLT31_06660"
     CDS             complement(3725..4102)
                     /locus_tag="PLT31_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94854.1"
                     /translation="MKRFAFLVIVVSLLTGCLSHWFTETEVRLQIENKTPYTIVGLQV
                     VSLDSTMKKNWIQDSIPPGERSSVVAGDWIGSFNIGVSYLKTPKNAEKKVFLHVFESV
                     KLDGGSIFAQISEKDGFWSFDQK"
     gene            complement(4099..5199)
                     /locus_tag="PLT31_06665"
     CDS             complement(4099..5199)
                     /locus_tag="PLT31_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94855.1"
                     /translation="MIFRKPVFLLFLLLNVAFLFADPVSVNSIHETGSTTRDRQLREF
                     LQAFRPGVSSYFNFGINYTPLSSQLPLESPFGIHDGYAFSQHATVFISMPAFEKKIVD
                     LGAFWSAERHGWDTEDFLFFPAYEKFSFIRSVQTGGFTFSYPKIKLGAALGVQYQNIE
                     KTNEVYPDESDSLYFWGHAYFGKVALQTSFHKADWRHVRVGIDLESKAVLGGDSSGWK
                     TYLPNFDVALFNGDEDDSVKITWEQNLYKQMLYSRVSAFLPDHGFSSASLTFYPDPSR
                     LFSLEASMFKKENGDFVFGGGIDLPFLRVAYNQAYDYENFFGAKGTVIVELHFALSSI
                     EKKFFGLNAAKPAPMEDQQIRIPSAKDFGGAK"
BASE COUNT         1751 a         1289 c          996 g         1693 t
ORIGIN      
        1 accgttgata actctattta tgcagaatat ggcatcaaaa gtattttagc gtgaatgggc
       61 atttactttc tggaaagccc aaacagaaag ggatctattt gcgaaaagaa tttttccgca
      121 ataagaaatc cactctatcc atcattagaa attaaactgc aaagccaaca gcatcatcaa
      181 gcttcacaga agcgattctt gaaataccct taatctcttg cgtaacacca tagagcatat
      241 cggcagaagc catggtacgc ttattatgcg tgaccaaaat aaactgggtt tgctttgaaa
      301 aatggcgaag caattccata aaacgcccaa tattggcatc atctagaggg ccgtcgactt
      361 cgtccaagac gcagtaaggc gaaggttttt ccatgtaaat cgcaaacaag agagacaccg
      421 ctgtgagcgc gcgttctcca ccagagaggg ctctcacgcc acgcatcttt ttgcctgtag
      481 ggcgagcatt gacttcaatt tcggcttcga gaggatctac gccttcttct agcgtgagct
      541 ttgtaccgcc atcatgcatt aaacggctaa agacttcttg gaagttcttc tgaattttat
      601 ggaacgtatc cataaagcga tcacgagcaa taatatcgag tttatgaatc gtacgttcca
      661 aagaagctct tgcgcgatcc aaatcatcaa actgcctttc gacttcttct aaacgatgtt
      721 tttcttcttc aaaatcgtcc ataatgcctt ggttcacagg gccaagtgcc ttaatttttc
      781 cacggatttc tctgatttca cgatctgcag aagagacttc gtattctaca cgaactacat
      841 catcggtatt gtcgagatcg acttcccatt cactaaaaat acgttcacgt agacgctcga
      901 tatttgcact taaagattct aagcggcgtt caatatcttt taattccgaa atgcgctcca
      961 caagagaatc attaatggag cgttcttcgc cacgccattc ttctaaatcg ccagagacga
     1021 cattatagcg ttcacgcgct tcatctcggc gacgttctaa tacgcgcaaa gcctcgtgct
     1081 tttcttcaat ctgagaagca agcgtctcgg actcttgcgt gacttgagtg gccttaatct
     1141 caatgccttc aatttcttgt tcgcgcgtta aaatcagttc ttcaagcata gaaagctgtt
     1201 cgccaatcga attcaatctc gaaacgcttg ccgaaatcag agaacgtttt tcccctaacc
     1261 gcatttcaat ttcgcgaata tcgctttctt tattacgata catcaattct tgttcttgcg
     1321 cttttgcaag cgttttttga tattcatctt ctacttgagt cgcaagagtt tctgcttctt
     1381 ttaattcgct atcttcacta cgctctgatt ccatagccga aattcgagat ttcgcgcggg
     1441 agcgttcgtt ttcaatgcgt tctaattgac gatccatatt ctgaatcgta tgctggtgaa
     1501 tcaacttcgc tgcatttcca gactgaattt gagcttgctt ttcacgaata tcttctaaaa
     1561 tcgaattgaa aaccgtttga tcttcgtcta aaagatcttg ggagcgatta taagaggttt
     1621 caatcgattg caactcgctc ttaatttgat caagcttctt ttctaattcg tcaatttctg
     1681 tacgacgaga taaaatgcct aacgattctg aagagccgcc tcttgcacga ataaaaccag
     1741 cagtatgaac cgcacgacca ggagacaaga accataaatc tttggaggca ttttcttttg
     1801 ccaaacgacg agccgtttca aaatcttcga ctaaaatcca tttagaaagc cacctttcta
     1861 ataatggacg aatttctgga gcagcagaaa caaaacgatg cgcagggcca atcacacgat
     1921 cgccttcgat agaagaagat tcaaaagagg attctcctgc aaaacttgca agcaacacac
     1981 tccccacaga agcttgttct aaagcattta agagagaatc agcgagattt gcatcttgta
     2041 aaagaaccgc atctaaggct tctccaagag caaactcaat ttgtgaaaca taacgaggat
     2101 cagcctcaat cttttgccct aaaaggcctg caatttcttg agatttattt tctaaaagcc
     2161 acttggcccc ttcgccttct gaaatagcca tactagaaag aacatcaaaa cgagattgca
     2221 aacgcatttc ttcgttttta tagttttgca aacgcttttc cagcttttga atttcctctt
     2281 tagaagactc caccttttct gcttgcacgc actgacgttc ttcaagcatt tgaagctcac
     2341 gttcggcttc agcaatgcct tcaagcatcg cctctaaatt accttgtgct tctttctttt
     2401 gctcttctat atccgaaaat tctttatcgc gattttcaat atcttttaag agaagatctt
     2461 tttctgtatc tgttctttgc cagcgagaac gcaaactatt tgccttattc aacacttgaa
     2521 tacgcttttc agcaagttcc tgagacgatt ttctagaatc atcataagca ttgcgtaaaa
     2581 cctgcaacac ttctcgctct tgttccaata aacgcacttg ctcatccatt tcatcgtcag
     2641 agcccaaatg cgctgttttt tcttctaaca attctttttc tgtacgaagc gtcgtttgct
     2701 tctcgcgaga ctcttcaatt tctttacggt acttattaga agactcttcg agagtcacaa
     2761 gcgtttctct aaaacgcatc aattcattat tcatattgtt caaagagagt tcttcttttt
     2821 gaaccaattg ttctagctcg cgtaaactat tttcatcatc gctaatcgca aggcgtttct
     2881 catcaatttg agtttttaag accgttagcc ttgttttaga cgattctaaa tcgtgctcca
     2941 ctcgttttct tgaagtatct aaaatagaaa cgcttttttt attgtcttca tacttatcta
     3001 aagaaagcga taaatcaagt tcttttaagc ggcggcgtaa ttccatccac tcgcgtgttt
     3061 tctcggcttg tttctcgtac tgccgaacac tgcgccttgt actatttaag ttgtcttcta
     3121 cacgagccat atccacttga acacgctcta attgacgaag agtttctttt ctttgttgct
     3181 tatacttact cacaccagcc gcttcttcaa aaagagtgcg acggtcttcg gctttatcag
     3241 ataaaacggc attaatcatc ctctcgttca tctgagagta tgttcctgta cctaagccag
     3301 aatcaaaaaa taagttttga atgtctttta agcgacattc ctgattgtta attaaatact
     3361 cgctcgttcc attacgatgt gctcgacgag tcaccataat ttctgcataa ggaagaggta
     3421 acctaccgtc atcattatta ataactaagg aaacctctgc aagactcatg gcagcacgct
     3481 cttcagtacc agagaaaatg acatcttgca tttttcccat acgaagaatg gcggcctttt
     3541 gttctcctag aacccaacga atagcatcca caatattaga cttaccgcat ccattaggac
     3601 caacaaccgc ggtcagacca ttacccggaa acgtaatctc cgttctctga gcaaaggact
     3661 taaatccaaa tatttttagt ttcgttatat gcacaagtag ccaataataa aattttataa
     3721 gaacttactt ttggtcaaaa ctccaaaagc cgtctttttc agaaatttga gcaaaaatac
     3781 tgccaccatc tagtttcaca gactcaaaaa catgaagaaa aacctttttt tcggcatttt
     3841 ttggggtttt aaggtaagag acccctatat taaaagaacc tatccaatca ccagcgacca
     3901 ccgatgaacg ttctccagga ggaatcgaat cttgaatcca attttttttc atggtagagt
     3961 ctaaagaaac gacttgtaag cccacaatag tataaggtgt tttattctca atttgaaggc
     4021 gcacttctgt ttctgtaaac caatgagaaa ggcatccagt caacaaggaa accacaatca
     4081 ctaaaaaagc aaaacgtttc attttgcacc tccaaaatct ttagcagaag ggattctgat
     4141 ttgttgatct tccatagggg ctggcttagc tgcatttaag ccaaaaaact ttttctctat
     4201 agaagaaaga gcaaagtgca attccacaat cacagtcccc ttagcgccaa aaaagttttc
     4261 ataatcataa gcttgattgt aagcaacacg taagaacggc aaatcgatcc cgccaccaaa
     4321 aacaaagtct ccgttttcct ttttgaacat cgaagcctct agactaaata acctagaagg
     4381 gtctggataa aacgttaaag aggcagaaga aaacccgtga tctggcaaaa atgcactgac
     4441 tctagaatac aacatttgct tataaagatt ttgttcccaa gtgattttaa ctgaatcatc
     4501 ttcatcgcca ttaaaaagag ccacatcaaa attaggaagg taagttttcc aaccactcga
     4561 atctccgcct aaaacagcct tagattctaa gtcaatccca acacgaacat gccgccaatc
     4621 ggctttatga aatgatgttt gaagagcgac ttttccaaag taagcatgcc cccaaaagta
     4681 aagagaatct gattcgtctg gatagacctc atttgttttt tcgatatttt gatattgcac
     4741 tccaagtgca gcgcccagtt tgattttagg ataagaaaaa gtaaagccac ccgtttgaac
     4801 gcttcttata aaagagaatt tttcatacgc tgggaaaaat aaaaaatctt cggtgtccca
     4861 accatgacgt tcagccgacc aaaaagcccc aagatcaact atttttttct caaatgcggg
     4921 catagaaata aataccgtag catgctgact aaaagcataa ccatcatgga ttccaaaagg
     4981 agattctaaa gggagttgag aagataaagg tgtataattg attccaaaat taaaatagga
     5041 gctaacccca gggcgaaaag cctgcagaaa ttctcgaagt tgacggtcac gtgttgtaga
     5101 gccagtctca tgaatgctgt taacagaaac agggtctgca aatagaaacg ccacattcaa
     5161 gagaagaaaa agaagaaata ctggttttct gaaaatcata ccaaaaatat acttactttt
     5221 aacccagata aaaaacaccc ccctaccctt gcttcgtttt ttccatgaag aaccaagcat
     5281 catttggtta taaagcaaat agcgcagatt caaatgaaaa aacaatacca ttcatattaa
     5341 ctgatacaat taaaatagca gacgggaatg gttgttattg atgagctcgt ttgtaccaca
     5401 agggtatcat taccacaggg gtatcactct tactaagcgc agctatgcat ggttcgacta
     5461 cagcgctaaa gcgctaagtc ttacgtaaaa gcgcaagtgt acgtgtttct cacataagaa
     5521 gtaaacttct aagtgttcgt gtttatggca cacaacccga gcgaagagat aacaccatta
     5581 ccgtcaacaa gagtagtttt aagatttaat cgtaatgaat aatgactttt attgaaaaaa
     5641 aaatcacttt cccatgaaaa aaacatctcc cattttcgtt taaacattag aacagaaact
     5701 gattctgttc ttttagccga gaatttcgg
//
LOCUS       DAOOBB010000132         5713 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_16260_length_5713_cov_4.671439, whole genome shotgun sequence.
ACCESSION   DAOOBB010000132 DAOOBB010000000
VERSION     DAOOBB010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 5713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5713
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16260_length_5713_cov_4.671439"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..1254)
                     /gene="mqnE"
                     /locus_tag="PLT31_06670"
     CDS             complement(214..1254)
                     /gene="mqnE"
                     /locus_tag="PLT31_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HPW94856.1"
                     /translation="MERISVEEALDLFLNAPLEELCERANLEKQARHGNSVFWVNNRQ
                     INYTNICVLHCNFCAFSKIKKDSPHAYDWNHEVVLEKAAEAVSKGARELHIVGGLHPD
                     HPFSYYVEMLSKLRKAFPLVNLKAFTAVELCYFAKKSERALDDIIHELKVAGLDALPG
                     GGAEILVQSVRDEICSGKETGEEWLNAHRAVHKAGIFSNATMLFGHIETPEQRIEHLN
                     LLRQLQDETHGFYAFIPLVFHPEHNSLGRRIPQITSEEDILRTVAISRLFLDNFPHIK
                     AYWIQMGIPTAMKALHAGASDLDGTIIEEKITHAAGAASPVGMTPEHIQELIRAEGLI
                     PVERDAKYEIYS"
     gene            complement(1264..2454)
                     /gene="metK"
                     /locus_tag="PLT31_06675"
     CDS             complement(1264..2454)
                     /gene="metK"
                     /locus_tag="PLT31_06675"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014544905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HPW94857.1"
                     /translation="MANYLFTSESVSKGHPDKVSDQISDAILDAALSSDPNSRVACET
                     LVNTGLVVIAGEITTNATIDYPKIAREVIKEIGYTDSEMGFDYKSCAVLVSLDKQSPD
                     IAQGVDAQKAAGKATAEQGAGDQGMMFGFACNETKELMPLPISLAHELMAKIQELRES
                     GEIPWLRPDAKSQVTVEYDENDKPIRVDTVVVSTQHQASYKGKEITHELIEKAIIQKL
                     IKKVIPANLLDKKTRFLINPTGKFVVGGPHGDCGLTGRKIIVDTYGGMGRHGGGAFSG
                     KDPSKVDRSGAYAARYVAKNIVAAGLATRCEVQLAYAIGYSQPVSVLVNTFNTGKIAD
                     SQIEELVKKHFDLSPAGLTKMLDLKKPGYLSTAALGHFGRTGKRFTWEKTDKASALKK
                     AASL"
     gene            2736..3497
                     /locus_tag="PLT31_06680"
     CDS             2736..3497
                     /locus_tag="PLT31_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014544906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HPW94858.1"
                     /translation="MLLRDFSNWLSDLLEPSKYKDYCSNGLCVEASSEVTKVVTGVSF
                     RESLIDKAIELGADCIIVHHPHGFWASDSKLPIGILGRKVQKLMENHISLFGFHLPLD
                     GHPEIGNNALIASKLGMERISGFMSSGEKEVGCIASFPKPISREEVFMRIQKLFPLGV
                     SHSLFYGSSEIQKIGICSGGGSSGIDEAFSLGVDLYLTGEIKEQTPIFVEEERQNVVA
                     CGHHRTEIFGVQALAKKIEVDLKIPSTFIDIDNAV"
     gene            complement(3517..4098)
                     /locus_tag="PLT31_06685"
     CDS             complement(3517..4098)
                     /locus_tag="PLT31_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94859.1"
                     /translation="MKSLFSFIIGFSSVLLFVGCAGTQDGSDLEVPTNMPPICRDIDF
                     VAQPDMREMCGVRTVRYKAYRNIPQQRYLIKPKETSIVKVRDVIELRFPNTLPIILDG
                     PIVNEIQFNQDKRLQKIKNSYDYYELYNEGKERIRIFKMGIPTDMGTFHEFCFRVPDK
                     VGNERTRSVAMGNRIEAMTCEDFHRLLTLNLKK"
     gene            4355..5158
                     /locus_tag="PLT31_06690"
     CDS             4355..5158
                     /locus_tag="PLT31_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HPW94860.1"
                     /translation="MNIYSWNVNGIRSIAKKGFEKWFSEVQADILCLQEVRADLDQIP
                     ESLQNPEGYFSYWNSSKKKKGYSGVAVYSQIEPDLVNYGMGISEFDDEGRMIQLVFPD
                     WVLNNVYFPNGGQGEDRLDYKLRFYDAFLENSLHWLSNGKHVVTVGDYNTAHQEIDIA
                     RPKENENISGFLPIERAWIDKYIKSGFVDSFRELNPSKTDAYTWWSNRFSSRERNVGW
                     RLDYGFVDKALMASVLDSKILSEVEGSDHCPICLELDPPFPPIKVVPTV"
     gene            complement(5155..>5713)
                     /locus_tag="PLT31_06695"
     CDS             complement(5155..>5713)
                     /locus_tag="PLT31_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HPW94861.1"
                     /translation="RCGWAEYYNRDKDEQFDAFCKALDNAKMIEVCSTTIPQLTIPKI
                     LGDPRYMVHRTTLNEKIGRRSSIINEILSDVPELNFNPTYGAFYNTITFKEGVLNDRQ
                     TLPINNPEIRAKVEGWVQNVPSLDYRFVYYLLGATGICVVPSSSFCSELQGFRVTLLE
                     ENDDELRHIFTILRDAIKAFIRSAS"
BASE COUNT         1752 a         1027 c         1133 g         1801 t
ORIGIN      
        1 cgcttagtac tttagtactt agcgagaatg atacccttgt ggtacaaaaa aaagcttgtc
       61 tcggcataaa aaaatggggc tagtatagat gaacgtctca ttttcgtaca tgcatggttc
      121 gcgaaaataa ttgccaaaaa cacaaacact tatgctgtag tcggaaccat gcacagttag
      181 tgataataat ccccgtggga aatgaataga atcttacgag taaatctcgt atttggcgtc
      241 acgttctaca ggaatcaagc cttcggcgcg aatgagttct tgaatatgtt caggagtcat
      301 tcccactgga gaagcagcgc ctgcggcgtg agtgattttt tcttctataa ttgtgccgtc
      361 taaatcagaa gcccctgcat gaagtgcctt catggcggtt ggaatcccca tttgaatcca
      421 gtaggctttg atgtgaggaa aattatctaa aaataatcta gaaatagcga cggttctaag
      481 gatatcttct tccgaggtga tttgaggaat gcgacgccct aggctattat gttctggatg
      541 aaacacgaga ggaataaagg cataaaaacc atgggtctca tcttgtagtt gacgtaaaag
      601 atttaaatgc tcgattcgct gttctggtgt ttcaatatgt ccaaagagca tcgtggcatt
      661 ggagaaaata cccgctttat gaacggctcg atgagcgtta agccattctt ctcctgtttc
      721 tttaccgctg caaatttcat ctcggacact ttgcacaaga atttcggcac caccccctgg
      781 cagggcatct aacccagcga cctttaattc atggataata tcatctaaag ctcgttcaga
      841 ttttttggca aagtaacaga gctcgacagc agtaaacgct tttaaattga ctagagggaa
      901 cgctttccgc agtttagaaa gcatttccac ataataagaa aagggatggt cagggtgtaa
      961 tcccccaacg atgtgtagtt ctctagcgcc tttgcttaca gcttctgcgg ctttttcaag
     1021 aacaacttca tggttccaat cataagcatg aggactatct ttttttattt tagaaaacgc
     1081 acaaaagttg cagtgtaaaa cacaaatatt agtgtagttg atctgccggt tattcaccca
     1141 gaaaacagag ttcccatgcc tagcttgctt ttcgagatta gctcgctcac aaagttcttc
     1201 taaaggagca ttgaggaata agtcgagagc ctcttctaca ctaatacgtt ccattgaaga
     1261 gtcctacaaa gaggctgctt ttttaagagc agaagctttg tctgtttttt cccaagtaaa
     1321 gcgttttcct gttcttccaa agtggccaag cgctgctgtg cttaagtagc caggtttctt
     1381 taagtcaagc attttagtta aaccagcagg agataaatca aagtgctttt taacaagttc
     1441 ttcaatttga ctatctgcaa ttttacctgt attaaaagta tttactaaaa cagaaactgg
     1501 ttgtgaatac ccaatagcgt atgctaattg tacttcacag cgtgttgcaa ggccagcggc
     1561 aacaatgttt ttagcaacat aacgagcggc ataagcacca gaacggtcaa ccttagaagg
     1621 gtcttttcca gagaaagcac caccgccatg acggcccatg ccaccataag tatcgacaat
     1681 gatttttctt ccagttaagc cacaatctcc atgaggacca cccacaacaa attttccagt
     1741 gggattgatt agaaagcgag tttttttatc gagcaaattg gctggaatta cttttttgat
     1801 caatttttga atgatggctt tttcaataag ctcatgagta atttcttttc ctttgtaaga
     1861 agcttggtgt tgcgtactta caacaaccgt atccactcgt ataggtttat cattttcgtc
     1921 gtattctaca gtgacttggc tttttgcatc tggacgtaac caaggaatct caccagactc
     1981 acgaagttct tgaatcttag ccattaattc atgagccaga gaaattggta aaggcattaa
     2041 ttcttttgtt tcattgcaag caaaaccaaa catcatacct tggtcgccag cgccttgttc
     2101 tgctgttgct tttccagcgg ctttttgtgc atcgacgcct tgtgcaatgt ctggagactg
     2161 tttatctaaa gagactaaaa cagcacagct cttataatcg aaacccattt cagaatctgt
     2221 ataccctatt tctttaatga cttctcgagc gatttttgga taatcaatag tggcgtttgt
     2281 ggtgatttca ccagcaataa caacaagccc tgtattcacg agcgtttcac aagctacacg
     2341 actattaggg tcagaagaga gagctgcatc taaaatagca tccgaaattt gatcggaaac
     2401 tttatctgga tgaccttttg aaactgattc agatgtgaaa agatagtttg ccatatttat
     2461 ccttacttaa gggctcattt atgcataaat ttataaaaac gcatagagtt tttcaatgca
     2521 atttcatccg aatttaaaaa atagtgatgg agtgattttt ttaatagctt tttcaaaaaa
     2581 aacagataaa gataccctaa ttgcaaaaaa actcttttta atctaattct cgaattttct
     2641 tcctttaatt agctgtttta aggtgagtaa gattaaaaaa cgatttttca tcatttcttt
     2701 ttttatttta taaaaaacgt ttttttagga tttttatgct tttacgagat ttttcaaatt
     2761 ggctttcaga cttattagaa ccttcaaaat ataaagatta ttgctctaat ggtctctgcg
     2821 tagaggcgtc ttctgaagtc acaaaggtcg taacaggtgt ttcttttcga gaatctttga
     2881 ttgataaagc gatagaactt ggggccgatt gtattattgt gcatcatccc catggttttt
     2941 gggctagtga tagtaaactc ccaataggta ttttagggcg caaagtacaa aaattaatgg
     3001 aaaatcatat ttctttattt ggctttcatt tgcctttaga tgggcatcct gaaataggaa
     3061 ataatgcttt aattgcttca aaacttggta tggaaagaat aagcgggttt atgtcttcag
     3121 gagaaaaaga agtcggctgt atcgcctcat tccctaaacc aatttctaga gaagaagtgt
     3181 ttatgcgaat acaaaaactt ttccctttag gggtttctca ttctttattt tatggttctt
     3241 ctgaaattca gaaaataggg atttgttctg gaggaggaag tagcggcata gatgaagcat
     3301 tttctttagg agtagacctt tatcttactg gagaaattaa agaacaaaca ccaatttttg
     3361 tagaagaaga acggcaaaac gtggttgcct gcggtcacca tcgcaccgaa atttttggtg
     3421 tacaagcatt agcaaaaaaa atagaagtgg atctaaaaat cccttctact tttattgata
     3481 tagataatgc tgtttgattt ttataaaaac agagctttat tttttcaagt tcaaggttaa
     3541 taaacgatga aagtcttcgc aggtcatggc ttcaatgcgg ttacccatgg cgacacttcg
     3601 agtacgttca ttaccgactt tatctggaac tctaaagcaa aattcgtgga aagtgcccat
     3661 gtcggttgga attcccattt tgaaaatacg aatgcgttct ttcccttcat tataaagttc
     3721 ataataatca taggaatttt tgatcttttg aaggcgcttg tcttggttga attgaatttc
     3781 gttaacaata gggccatcta aaataatagg aagtgtgttt ggaaagcgta actcaattac
     3841 atcacgaact ttaacaatcg aagtttcttt aggcttgatt aagtaacgtt gctgaggaat
     3901 attgcgataa gctttatagc gaacggtacg aacgccgcac atttcacgca tatctggttg
     3961 ggcaacgaag tctatatctc tacaaatagg aggcatattt gttggaactt ctaaatcgga
     4021 accgtcttga gtgccagcac acccgacaaa aagtaagact gaagaaaagc caataataaa
     4081 ggaaaataga gatttcataa atctaaatat aaacttaatc aaaaaaaggg ggtacgtttt
     4141 ttcttgcatt gaattgaaaa tagctgtttt ttatgtgata atcaaataaa aagtgattta
     4201 ttactatgag gataataaaa gcatcaaacg cttaaaagtg tttgtatttg cccttttttg
     4261 gataaacacg ttatgttata taattttttg cggaaaagaa ttaattattc tatctttgac
     4321 actctgatcc aaagaaaaaa aggttgttgt attcatgaat atttatagct ggaatgtaaa
     4381 cgggatccgt tcgatcgcaa aaaagggatt tgaaaaatgg ttttcagaag tccaagcaga
     4441 cattctatgc cttcaagaag tacgcgctga tttggatcaa attcctgagt ctttacaaaa
     4501 tccagaaggc tatttctctt attggaattc ttctaaaaaa aagaagggtt atagtggggt
     4561 agctgtttat tctcaaatag aaccagatct tgttaactac gggatgggga ttagcgagtt
     4621 tgatgatgaa ggtcgaatga ttcagctcgt ttttcctgat tgggttttga ataatgtgta
     4681 ttttccaaat ggagggcaag gagaagatcg tttagactac aagcttaggt tttacgatgc
     4741 ttttcttgaa aactctcttc attggctttc taatggcaag catgtggtga ctgttggcga
     4801 ttataatacg gctcatcaag aaatagatat tgctcgccct aaagaaaatg aaaatattag
     4861 tggcttttta ccaattgaac gagcttggat tgataaatat atcaagagtg gatttgtgga
     4921 ctcctttaga gaattaaacc cttcaaaaac agatgcctat acttggtggt ctaatcgttt
     4981 ctcatcaagg gaaaggaatg tggggtggcg cttagactac ggctttgttg ataaagcatt
     5041 aatggcaagc gtactagact caaagatttt atcagaagtg gaaggctcag atcattgccc
     5101 tatttgtctt gagctagacc cgccttttcc accgataaaa gttgtaccaa cagtttaaga
     5161 cgcacttcga ataaaggctt ttatggcgtc gcgtaagatg gtaaaaatgt gacgcaattc
     5221 atcatcattt tcttctagta atgtgacacg aaatccttgt agttcactgc agaagctgct
     5281 acttggaact acgcatatac ccgtggcgcc taataaataa taaacaaatc gataatctaa
     5341 actaggaacg ttttgaaccc aaccttccac cttagctcga atttcagggt tattaatagg
     5401 gagagtttgc ctatcattaa gaacgccttc tttaaaggtg atcgtattat agaaagcacc
     5461 ataagtaggg ttgaaattaa gttcaggaac atcgcttaga atctcgttaa ttatggaact
     5521 gcgacgtcca attttttcgt tgagagtagt gcgatgaacc atatagcgag gatcgcctaa
     5581 aatctttgga attgtaagtt gaggaattgt tgttgaacag acttcaatca tttttgcatt
     5641 gtctaaagct ttgcagaacg catcaaattg ttcatcttta tcacgattat aatattctgc
     5701 ccaaccacaa cga
//
LOCUS       DAOOBB010000133         5701 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_16298_length_5701_cov_4.977860, whole genome shotgun sequence.
ACCESSION   DAOOBB010000133 DAOOBB010000000
VERSION     DAOOBB010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 5701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5701
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16298_length_5701_cov_4.977860"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="PLT31_06700"
     CDS             <1..513
                     /locus_tag="PLT31_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HPW94862.1"
                     /translation="WSLDFDVKVYPAGSPDVSQQKGSLLVGEKNKFRLSVSGITFISD
                     GVSLWQWNIEQRQVLIKSIEDLSSALHPSELLFKYLNCRAISVREEQWKKKKVYVLTL
                     DPAKYGDQFKAMEVWLSAKDYSPVRLFTVDHIDNASQYEISNLKIVKKTTLADFTFKS
                     SKEIDEVDMR"
     gene            531..2111
                     /locus_tag="PLT31_06705"
     CDS             531..2111
                     /locus_tag="PLT31_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94863.1"
                     /translation="MLQKLRIVLFCFCSVFASESLVSNFTFAVARGPSTGTLWVLSRG
                     DAGSGVTLVTLVPGESQVNVVSMQSDYFDQEKTAVHDGIFSDVTGETRRTPFIYTEKL
                     GFVLPMFGLDSIDSFRNPEGIFSVKTSRVLETPLDEPLVEVDSAYSFPMAKSIGSFAY
                     DTSTSVLWISRGILGLERYDVSASVIDPKTSYFMLNGENGSLDTLSRNDKIKASEHPP
                     IYGLALHPETSMLWLGTEKGLYHQTAARSFKKIDALSDLRITGVWIGGSPVQVIVEAS
                     AREKSVTKSSLWRSWNGSDFKEVVFRDTLGKIQKNVYDKADYSVSNVAFIQNKAFLAV
                     QTIEGSISGLLKLDSLGAIPWSYSRQWLYDVNAGVLNSNSILTSVAAIPLTDSIMGLA
                     VSSYGSGISVSADSGLTWSHILNQAAVKGDLGSIRMVPSVIVAGGESLVAYKVGKPSK
                     ITIEVFSYDMRRVRTIVKGAPREKNAVRSSKATEDFWDGYDEHGRPCAMGIYYVRVKD
                     NHGHVGWGKVMTLGGNKK"
     gene            2108..3130
                     /locus_tag="PLT31_06710"
     CDS             2108..3130
                     /locus_tag="PLT31_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94864.1"
                     /translation="MKKIALFCLLLVAFVYADKKAMLGSFDGFVERMGASAHELGRGN
                     TGSADTAAQPGAYWNPALLSFKTQLAYNLHAEKRDLDRAGGSLGVEGRVGKRMGVGFA
                     MLYRGDHSFDVVSDDDETLGNTSPYFTIMYLGFAYRVSRRDGIGVSFSMSYDNLNISE
                     YYDDISLVDSYRSPVSFNLGWVRQWNKKWSTSVVIRNLSFSKNLSAVWTRNPSTDNSV
                     ISTEGVRPKVLQIGLGYRSMLFGKPISAWMEALDYQIADTLLAFDPDRHVWTGRVGFE
                     YEVIPQGSVSIGMNDMNFSLGLGYQFNIRIGKKRWPFDVSYALLYESKANLWNPLSFA
                     VRGWIP"
     gene            3088..4203
                     /gene="recF"
                     /locus_tag="PLT31_06715"
     CDS             3088..4203
                     /gene="recF"
                     /locus_tag="PLT31_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546725.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HPW94865.1"
                     /translation="MESIKLCCARVDSLIQSIHIETARNINAMDVSFQPGITVVSGKN
                     GCGKTTLLEMIHVLCHGYSFRTRDLKELVSWNSKELLSRAFFLGERESVRALRIDSSG
                     ILAKKDTTESRSTSLFFGEIPAIVMQPSDISLVRGGPEERRRWMDEMLCIRSSLNTDL
                     LRRYRRVLLQRNQWLKQNRSGSPTGGEALFSVLTEQYIELAIKIWRARLDLVAEIGDI
                     TSDYYKLLAAGADEITVSYRSAVGKEKKTLDLERDFREALEASDDVERLQGITQIGPH
                     KDDFILWSSGYELRSVGSQGQCRSAAISMRFAALDLACKNKCPSILLLDDVFAELDEY
                     RRRAVADLIREKHAQVFIATPRLQDIPFTPDAQIEMG"
     gene            complement(4214..5659)
                     /locus_tag="PLT31_06720"
     CDS             complement(4214..5659)
                     /locus_tag="PLT31_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94866.1"
                     /translation="MSKLFLTLFRILSKAQWFLLLFAITLCIFNIVLWSEPYIADYQR
                     QQKIESIFSKYWQKEGAEKFRSVGLEPTEQLYQEELENYRTSFLEKNPPLIPEERISK
                     MKDDFRKWWETGGNLTYVNQGIVPDSKLYNRELKKWVEAYTQQLWTYTIPFIPAEGNF
                     VNLLIYWILMPSIFSLLSFFVLFCFTGYHLEKRWGVLKTLGVFISANIIGGFFVNLLS
                     TTTFFHSYENDLLKGMSLGLATLLGATSMGKTKNEISKIVVVVSGLLLMVDIAINWFL
                     NPGIYAAVVILSLFFYGIGLLLGLKMPYRKQTLDELRKDLLEEKRRNRVDPYVLKKNK
                     TRENLMNGLSAARRGENTAGGQLLTDGMQALLQEYPIDTELLNSTAKEMVSPSLFIEI
                     ISTQWLEWGVAANNKNIPEVALLFLEKSLATEKSESMARKALFQIGEIRIRCDINAEE
                     GALRLQKVLELNDKDIIAAQARKILEKRKSF"
BASE COUNT         1653 a          932 c         1211 g         1905 t
ORIGIN      
        1 tggtcgttag attttgatgt gaaagtttat cctgcgggtt ctcctgatgt ttcacaacaa
       61 aaagggagtt tacttgttgg tgaaaaaaat aagtttcgtc taagtgtttc agggatcact
      121 tttattagtg atggggtttc tttatggcaa tggaatatag agcaaagaca agtccttatt
      181 aaatcgattg aagatttatc aagtgcttta catccatcag agcttttatt taagtattta
      241 aattgtcgtg ctatttcggt tcgagaagag caatggaaaa aaaagaaagt atatgttcta
      301 actctagacc ctgcaaaata tggtgatcag tttaaggcga tggaagtttg gctttcagca
      361 aaggattatt ctcctgtacg cctttttacc gtagatcata tcgacaatgc ttctcagtat
      421 gaaatttcaa atttgaaaat tgtaaaaaag acaactttag ctgattttac ttttaagagc
      481 tctaaagaaa ttgatgaagt ggatatgaga taagaagggt taggtttttt atgcttcaaa
      541 aattgagaat agttcttttt tgtttttgtt ctgtttttgc ttcagaatct ttagtgagta
      601 attttacttt tgctgtggcg agaggccctt cgacagggac tctttgggtt ctttctcgtg
      661 gagatgcggg gagtggagtg actcttgtga ctttagtccc tggagaatct caggtgaatg
      721 tggtttcgat gcagtcggat tattttgatc aagaaaaaac ggctgttcat gatgggattt
      781 tttctgatgt cactggagaa actcgtcgta ctccatttat atatacagaa aaattagggt
      841 ttgtcttacc tatgtttggt ttagattcta tagatagttt tagaaatcca gaaggcattt
      901 tttctgtcaa aacgagtcga gtattagaaa cgcctttaga tgaacctttg gtagaagttg
      961 attccgctta ttctttccct atggcaaaaa gcattgggtc ttttgcttat gatacgagta
     1021 catcggttct ctggatatcc agaggtattt tagggttgga acgttacgat gtttctgcct
     1081 cagtaattga ccctaagact tcttatttta tgctgaatgg tgaaaatggt tctttagata
     1141 cgcttagtcg taatgataaa attaaagcaa gtgaacatcc tcctatttat ggacttgctt
     1201 tgcatccaga aacatccatg ctttggcttg gtacagaaaa aggtctttat catcaaacgg
     1261 ccgctcgctc ttttaaaaaa atagatgcat taagtgattt acgcattaca ggcgtttgga
     1321 taggtggttc tccagtacaa gtgattgtgg aagcctctgc tcgagaaaaa tctgtcacaa
     1381 aaagtagtct atggcgttct tggaatggat cagattttaa ggaagttgtt tttagagaca
     1441 ctttaggcaa aattcaaaag aatgtatacg ataaagcaga ttatagtgtt agtaatgtgg
     1501 cctttattca aaacaaagcg tttttagcgg tacaaactat agagggttcg attagcggtc
     1561 ttttgaagct agattctctt ggtgcgattc cttggagtta ttctaggcaa tggctttatg
     1621 atgtcaatgc aggagttcta aatagtaatt ccattttaac ttctgtcgct gcaattcctt
     1681 tgactgattc tattatgggc cttgccgttt cttcttatgg aagtggcatt tctgtttcag
     1741 cagattctgg ccttacttgg tctcatattc taaatcaagc tgcagtgaaa ggcgatttag
     1801 gctctattcg aatggttcct tctgtgatcg ttgctggagg agaatcactt gtcgcttata
     1861 aagtgggaaa gccttcaaag atcacgatag aagtatttag ttatgatatg cgtcgagtga
     1921 gaacgatcgt taagggagcc cctcgtgaga agaatgcagt gcgtagctct aaagcgacag
     1981 aagatttttg ggatggatat gatgaacatg gaaggccatg tgcgatgggt atttattatg
     2041 taagagtgaa agataatcat ggtcatgtag gttggggaaa agtgatgact cttggaggga
     2101 ataaaaaatg aagaaaatag ccctcttttg tttgttgtta gtcgcttttg tttacgcaga
     2161 taaaaaagcc atgttaggta gttttgatgg ttttgtagaa cgcatgggcg ccagtgccca
     2221 tgagttaggc cgcggcaata caggctctgc agataccgct gctcaaccag gtgcttattg
     2281 gaacccagcg ttgttaagtt tcaaaacgca attagcctat aatttgcatg ctgaaaaaag
     2341 agatttagat cgcgcgggag gctctttagg agttgaaggg cgtgttggta aacgtatggg
     2401 cgttggtttt gcaatgttat acagaggaga ccattctttt gatgtggtga gtgatgatga
     2461 tgaaactctt ggcaatacct ctccttattt taccatcatg tatcttggtt ttgcctatcg
     2521 tgtttcgcgc cgagatggta taggtgtttc tttttctatg agttatgata atctgaatat
     2581 ttcagaatat tatgatgata ttagtttggt cgatagttat cgaagtccag ttagttttaa
     2641 tttaggttgg gtgcggcagt ggaataaaaa atggtccact tctgttgtga ttcgaaattt
     2701 aagttttagt aaaaatttat ctgcggtttg gacacgtaat ccaagtacag ataattcggt
     2761 gatatcaaca gaaggagtgc gtcctaaagt attacaaata ggtttgggat acagaagcat
     2821 gctttttgga aaacctattt ctgcttggat ggaggcttta gattaccaaa ttgctgatac
     2881 tcttttagct tttgaccctg atcgtcatgt atggacaggg cgagttggtt ttgaatatga
     2941 agtaatacct caaggttctg taagtatagg tatgaacgat atgaactttt cattgggttt
     3001 agggtatcaa tttaatattc gcattggaaa aaaacgctgg ccttttgatg tgagctatgc
     3061 cttattatat gaatcaaaag ccaatttatg gaatccatta agctttgctg tgcgagggtg
     3121 gattccttga tccaatcgat tcacatcgaa actgctcgaa acataaatgc catggatgtt
     3181 tcttttcagc ctggcattac tgttgtgagt ggaaaaaatg gttgtggcaa aacaacttta
     3241 ttagagatga ttcatgtact atgtcatgga tactctttta gaacgagaga tttaaaagaa
     3301 ttagtttctt ggaattccaa agagctcttg tctcgtgctt tttttttagg agaaagagaa
     3361 tcagttcgcg cgcttcgcat tgactcaagt ggtatattag caaaaaaaga tacgacggaa
     3421 tcgagatcaa caagtctttt ttttggagaa attccagcaa ttgtcatgca accatctgat
     3481 atttcattag tgcgtggtgg tccagaagaa cgtcgccgtt ggatggatga aatgctttgt
     3541 attcgttcat cactcaatac agatctttta aggcgatacc gtcgtgtttt attgcagcgt
     3601 aatcaatggc tcaagcaaaa tcgttctggt tctcctactg ggggagaagc tttgttttcg
     3661 gtgctcacag aacagtatat tgaacttgcc ataaaaattt ggcgcgcgcg tcttgatctt
     3721 gtggcggaaa taggggatat cacctcggat tactataagc ttttagctgc gggagcagat
     3781 gaaataactg tttcttatcg aagtgcagtt ggtaaagaaa aaaaaactct tgatctcgaa
     3841 cgtgattttc gagaagcatt agaagcttct gatgatgttg aacgccttca gggcataact
     3901 cagattggcc ctcataaaga tgactttatt ttatggtctt cgggttatga actcagatcc
     3961 gttggttctc aagggcaatg ccgttctgca gcaatttcca tgcgttttgc tgcattagat
     4021 ttagcatgta aaaataaatg cccttcgatt ttattactgg atgatgtttt tgcagaatta
     4081 gacgaatacc gtcgtagagc tgtggctgac ttgattcgag aaaaacatgc ccaagtcttt
     4141 attgctactc cgcgtcttca ggatattccc tttacaccag atgctcaaat agaaatgggt
     4201 taagaaagcc tcattaaaag gactttcttt tctctagtat ttttcttgct tgggctgcaa
     4261 taatatcttt atcattgagt tcaagtactt tttgaagtct gagagcacct tcctctgcat
     4321 ttatatcaca acgaattcga atttcaccaa tttgaaatag agcttttctt gccatagact
     4381 cacttttttc agtggcaagg cttttttcaa gaaagagaag agctacttca ggtatatttt
     4441 tgttattggc ggcgacaccc cattctagcc attgagtgct tattatttct ataaataaag
     4501 aggggcttac catttctttg gcggttgaat tcaatagttc tgtatcaata ggatattctt
     4561 gtaaaagggc ttgcattcca tcagttaata actgcccccc tgctgtattt tcgccacgtc
     4621 ttgctgccga aagaccattc attaggttct ctcgcgtttt attctttttt aagacgtacg
     4681 gatcaactct attgcgacgt ttttcttcta aaagatcttt tcttaattca tcaagagtct
     4741 gttttcgata aggcattttt agcccaagta aaagaccaat accatagaag aataacgata
     4801 agataacgac agcggcatat attccaggat taagaaacca attgattgca atatcaacca
     4861 tcaaaagtaa gccagaaaca acaaccacta ttttggaaat ctcgtttttg gttttaccca
     4921 tggaggttgc acctaaaagc gttgctaatc ctaaactcat gcccttcaaa aggtcattct
     4981 cgtaggagtg gaagaaagta gtggtagaca gtaaattaac gaagaatcca ccaattatat
     5041 ttgctgaaat aaagacacct aatgttttta agacacccca tcgtttttct aagtggtagc
     5101 ctgtaaaaca aaagagaacg aaaaatgaca ataaagagaa tatactaggc attaaaatcc
     5161 agtatattag aaggtttaca aaatttcctt ctgcagggat aaatggaatt gtgtaagtcc
     5221 ataattgttg agtgtaagct tccacccatt tttttaattc tctattatag agttttgaat
     5281 ctggcacaat gccttgattt acgtaagtta gatttccacc agtttcccac cattttctaa
     5341 aatcgtcttt cattttagaa atacgttctt caggaattaa cggaggattc ttttcgagaa
     5401 aactagttct gtaattttca agctcttctt gatacagttg ttccgttggc tctaaaccaa
     5461 cacttctaaa cttttcagct ccttcttttt gccagtattt tgaaaatatg gattcaattt
     5521 tttgctgacg ttgataatca gcaatatagg gttcgctcca taaaacaata ttaaaaatac
     5581 agagagttat agcaaataaa agcaaaaacc attgtgcttt tgacaatatt ctaaataagg
     5641 ttaaaaaaag tttactcata aaatgaatat attcaaaaaa gaaaaataaa aaagtgtttt
     5701 t
//
LOCUS       DAOOBB010000134         5486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_17059_length_5486_cov_4.725281, whole genome shotgun sequence.
ACCESSION   DAOOBB010000134 DAOOBB010000000
VERSION     DAOOBB010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 5486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5486
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17059_length_5486_cov_4.725281"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /locus_tag="PLT31_06725"
     CDS             complement(<1..1188)
                     /locus_tag="PLT31_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94867.1"
                     /translation="MIFSFKMALHFKLSIFVLLCFSSLFAVEKIDCIDPEGEYDQRCI
                     YVAIGDVKGTNLFVVETGMSLVRKNALPNEDFYVFHPTVESSNALFPKDSLRVSLINE
                     SSKDSLLTTNNNPVNSGPDSIVTLKVVADYATYNSPVGTAIKGSDANIVRIYNFYVPV
                     LKYFDKDGKAISEETILSGEVGSEIEIEVRAVIPIGPKTQQLDSSINDKDFFIHVPSS
                     SPLGFYNDKGESLNLNDSTVLIKLFNGSTNIIVKADKAVDGADFKVEGYPRETSTGTN
                     TFLVSEAFPGGLTFVNKGLPTLDSASIFDSNGDGIGDSIVAWFSGETDAVTMVAGGTE
                     YSWPDGESFVTFGGDYLYNESKGILQLIDVETTYPKDSGEGILRVSVEAKAGEAKAIL
                     EADI"
     gene            complement(1384..2532)
                     /gene="hemW"
                     /locus_tag="PLT31_06730"
     CDS             complement(1384..2532)
                     /gene="hemW"
                     /locus_tag="PLT31_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HPW94868.1"
                     /translation="MLSLYIHTPFCAKVCHYCDFSVLSAPERLFEEYLILLEKEIEIM
                     LRRYPEFIDNTKTIYWGGGTPSILKIEQQNKVFQALEQQGISLFSLLEFSMEFNPESC
                     FQDRVENAIHHGVNRFSLGLQTFQENLLLRIGRSHDVQTAKTALEKLISLKSEGISVS
                     GDLMFNLPGQTTEDFLSDVRFLADQDVDHISFYGLNVAPLTVLGKRIERGSEKIDDIV
                     YEPMYLGGVEILTNANFERYEVSNFAKPNKQSLHNQNYWRGGSYLGFGPGAHSFVGSK
                     RFNAPEKYAPWRQWVLEGCPESKLNVDLIGKEERLTEKIWLSLRTSDGLDLKALEEES
                     VRIPEEVIQRWESKEMLACVDDHLHLQGRGWIFMDSIIEDFLIHNIPS"
     gene            2791..3876
                     /locus_tag="PLT31_06735"
     CDS             2791..3876
                     /locus_tag="PLT31_06735"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPW94869.1"
                     /translation="MMTRLTARKFKAICFYLFSWIIFCLLFALFNLYLNIEKNDTFSF
                     LFIFQFALLIGLSHGIYDVVVLEDDRDHRTIWVALLIRTSFFIVAILCNISICVFVWS
                     VYNTGEMISEESIMLLRIILKNPSVHLFMIYSFLMGYLITFVRSVHKKFGTRVFINTI
                     LGKYQEPTEEERIFMLIDLRNSTTLAEELGHFKYSNFLRDYYRFVSNCCEENRGEIYQ
                     IAGDGAYLTWPLKSCLKSPRPMLCFYDLQICLERTQKTFLKKYGVAPAFKAAVHCGKV
                     IVSEVGNFGSEIAYHGDVLNTTSRLETLCAKIGQEFVASEDFISKMPLIKMFYPQKQG
                     FFELKGKKKTISVYSLHFSLDKEKPLN"
     gene            3897..3969
                     /locus_tag="PLT31_06740"
     tRNA            3897..3969
                     /locus_tag="PLT31_06740"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3930..3932,aa:Gly,seq:gcc)
     gene            complement(4214..5125)
                     /locus_tag="PLT31_06745"
     CDS             complement(4214..5125)
                     /locus_tag="PLT31_06745"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HPW94870.1"
                     /translation="MRISNALLAFSFLATYSLAGQYWDVDPGGTTMKFLSTSVSPRSA
                     AIAGAGIVMPAGISEVSRNPLATTTVTQSAIGINEVIYSEAVGANLTSIYYGLPLESI
                     NVSAALEFLGYGEIEGRDDEGFKTSSYGAFSWAAQIGLSNRFSIFNWATTLRVASQTI
                     EDETALAILGDIGGSFLLNKYFSFGAVLTNVGYVSPYQTEEESPPTALQAGVSAKLPI
                     ASLFDFFAYADLHRRADAEPEWLFGGEINYKKILVLRGGYSLRPDTESGVSGGLGICF
                     DLFSIDYAYQSRPALEGNHLLSLTVFF"
     gene            complement(5253..>5486)
                     /locus_tag="PLT31_06750"
     CDS             complement(5253..>5486)
                     /locus_tag="PLT31_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HPW94871.1"
                     /translation="GESLFGKFSGKLISIDETDGYRMVFDSEEVIHLRPSGNAPEFRC
                     YVETASAERSKELLAKCLALMSEWVNANGKLGF"
BASE COUNT         1807 a         1071 c          873 g         1735 t
ORIGIN      
        1 aatatcagct tcaagaatgg ctttagcttc accagctttg gcttcgacac ttactcttaa
       61 aatgccttcg cctgaatctt ttggataagt tgtctctaca tctattaatt gcaaaatgcc
      121 tttcgattca ttgtaaagat aatctccgcc gaaagtgaca aaggactctc catcaggcca
      181 actatattct gttccacctg caaccatagt cacagcatct gtttcaccag aaaaccaagc
      241 gacaatacta tctccaattc catccccatt agaatcaaaa atggaagcac tgtctaaggt
      301 aggcaatcct ttattcacaa aagttaagcc acctgggaac gcctctgaaa ctaggaacgt
      361 attagtccct gtagatgttt ctcttgggta gccttctact ttaaaatcag ccccatccac
      421 tgctttatct gcttttacaa taatgttagt agaaccatta aataatttta taagaaccgt
      481 agaatcatta agattcaaag actccccttt atcattgtag aagccaagtg gagaagaaga
      541 gggaacgtga atgaaaaaat ctttatcatt gatagaagag tctaattgct gagtctttgg
      601 acctatagga attacagcac gcacttcaat ttcgatttca ctaccgactt ctccacttaa
      661 aattgtttct tcagaaatcg ctttgccatc tttatcgaaa tattttaata caggaacata
      721 aaaattgtaa atacgaacaa tgttagcatc gcttcctttt atggccgttc ccacaggaga
      781 attataggta gcataatcag ccacgacttt taaagtgaca atactgtctg gacctgaatt
      841 cacaggatta ttatttgtag ttaaaagaga atccttactc gattcgttaa tcaagctaac
      901 acgaagagaa tctttaggga ataatgcatt agacgattct accgttgggt gaaaaacata
      961 aaaatcttca ttaggtaaag catttttacg cactaatgac attcctgttt ctacgacaaa
     1021 aagatttgtt cccttaacat caccaatggc aacataaata caacgctgat catactcccc
     1081 ttcaggatca atacaatcaa ttttttcaac agcaaataga ctagaaaagc ataataatac
     1141 aaatatgctt aacttaaaat gtaatgccat tttaaatgaa aaaatcatct ttactacctt
     1201 gacaaaatac tgacacaaaa aaaacataaa accgcaacga ttaaaataaa ttctaaacca
     1261 aattaactgg tatgatcgca ttcacaccat tttgttcctt cagaaaaata cacaattttt
     1321 ttaaacaatt tattttttaa ttcgtttttt gatgaaaaat cagactaatt ctgttctctt
     1381 ggtctaactt ggaatattgt gaattaaaaa atcttcaatg atagaatcca taaatatcca
     1441 tcctcgcccc tgcaaatgta agtgatcgtc aacacaagca agcatttctt tgctttccca
     1501 acgttgaatc acttcttctg gaattctaac agactcctct tctaaagcct tcaaatcaag
     1561 cccatcagaa gttcttaatg aaagccaaat tttttcagtc agtcgttctt ctttacctat
     1621 taaatccaca ttcaatttag actcagggca cccctctaaa acccattgac gccatggggc
     1681 atatttttct ggtgcattaa aacgttttga acctacaaaa ctatgggctc caggaccaaa
     1741 gcccaaataa gagccccctc tccaataatt ctgattatgg agactttgtt tatttggctt
     1801 tgcaaaatta gatacttcat aacgttcaaa atttgcatta gtaagaatct ctactccccc
     1861 taagtacatt ggttcataaa caatgtcatc aattttttca ctacctcgtt cgattctttt
     1921 tccaagcact gttaatggag ccacatttaa gccataaaaa ctaatatgat ccacatcttg
     1981 atccgctaaa aaacgaacat ctgagaggaa gtcttcagtg gtttgtcctg gcaaattgaa
     2041 catcaaatca ccactcacac taataccctc tgattttaga gaaattagtt tttctaaagc
     2101 ggtctttgcc gtttgaacat catgagatct gccaattctt aataacagat tttcttgaaa
     2161 cgtttgaagc cctaaactaa aacgatttac tccatgatga atggcatttt ctactcgatc
     2221 ttgaaagcat gactcaggat taaattccat agaaaattca agcaatgaaa ataatgaaat
     2281 cccttgttgt tctagagctt gaaacacttt attttgttgt tcaatcttca aaatagaagg
     2341 agttcctcca ccccaataga ttgttttcgt gttatcaata aattctggat aacgcctcaa
     2401 catgatttca atttcttttt ctagtaagat caagtattct tcaaagagtc tctcaggagc
     2461 agaaagaact gaaaaatcac aataatgaca aacttttgcg caaaatggcg tatgaatata
     2521 aaggcttaac atatttataa taaaaaaaat aactcaatat ttaacaaata agaactcttt
     2581 taaatttgtt gatgtatatt gattttgtga aaaaaatgaa ctatgctgaa ataaaaaaaa
     2641 ttttatctgg acggctttca ctagttctgt ttttattttt atccttttta gctttgtttt
     2701 ttggctccac tttactgttg ggagagtctt ataaagatat tttcatcgtt attatgattt
     2761 ggtctcttcc tttattttta cattaacgct atgatgactc gtttaacggc aagaaaattt
     2821 aaggccattt gtttttacct tttcagttgg atcattttct gcctgttgtt tgcactcttt
     2881 aatttatacc tcaatataga aaaaaacgac actttttctt ttttatttat ttttcaattt
     2941 gctctactca ttgggctatc tcatggaatc tatgacgttg ttgttcttga agatgaccga
     3001 gatcaccgaa ctatttgggt agcccttctt atacgaacct ccttttttat cgtagctatt
     3061 ttatgtaata tttctatttg tgtctttgtc tggagcgtat ataatacagg tgaaatgatt
     3121 agcgaagaaa gtattatgct tctccgtata atacttaaaa atccatctgt tcatctattt
     3181 atgatttatt cctttttaat gggatactta atcacgtttg ttcgttctgt tcataaaaaa
     3241 tttgggactc gtgtatttat aaataccatt cttggaaaat accaagagcc cactgaagaa
     3301 gagcgaattt tcatgctgat tgacttacga aactcgacta cattagctga agaattaggg
     3361 cactttaaat acagtaattt tttaagagat tactatcgat ttgtttctaa ctgctgcgaa
     3421 gaaaatagag gtgaaattta ccaaattgct ggtgatggag cttatttaac ttggccatta
     3481 aaatcttgct taaaaagtcc tagaccaatg ctttgttttt atgaccttca aatatgttta
     3541 gaaaggactc aaaaaacatt tcttaaaaaa tatggagttg cgcctgcatt taaggcagca
     3601 gtgcattgtg gtaaggtgat tgtttctgag gttggtaatt ttggaagcga aatagcctac
     3661 catggagatg tgctaaatac gacctccaga ttagaaactc tttgtgcaaa aatagggcaa
     3721 gaatttgtcg cttctgagga tttcattagt aaaatgcctt taatcaaaat gttttacccc
     3781 caaaaacaag gtttttttga actgaaaggc aaaaaaaaga caatttctgt atatagtttg
     3841 catttttccc ttgacaaaga aaaaccatta aattaacttt gttcacactt aattacgcgg
     3901 gaatagctca gctggtagag cataaccttg ccaaggttag ggtcgagggt tcgaatctct
     3961 tttcccgctc tgagaaaaaa aaggtcacca ttaaggtggc ctttttttat gcttttttct
     4021 ctacaacttt aataaatagt attgtaagct atcagaatga atatgtgaca aatgaggtgc
     4081 aaaaatactt gaacgtcttt gcatccaaaa gcttttcaag cgcccctttt tttacaaaac
     4141 caagtccgta aaccacaaat aatccaaacc cagcaaggag actcaataga taaaaaagag
     4201 gatacgttat tttttagaaa aatacagtca aacttaaaag atgattacct tctaaagctg
     4261 gccttgattg ataagcataa tcaatcgaga aaagatcaaa acaaattcca agaccaccac
     4321 ttacaccgct ttctgtatct gggcgtagag aatagccgcc tcgtaaaact agtatctttt
     4381 tgtagttgat ttcgcctcca aaaagccatt caggttctgc atcggctctg cgatggagat
     4441 cggcataagc aaaaaaatca aataaagaag caataggtaa tttggcagag acccctgctt
     4501 ggagagctgt tggaggagat tcttcttcag tttgatatgg agaaacataa ccaacatttg
     4561 ttagaacagc accaaacgaa aaatatttat ttaagagaaa ggagcctccg atatcaccca
     4621 aaatagcaag ggctgtttca tcttctatcg tttgtgaagc cactctcaaa gtagtagccc
     4681 agttgaaaat agagaatcga tttgaaagtc ctatttgagc tgcccatgaa aaagcaccat
     4741 agcttgatgt tttaaagccc tcatcatctc gcccctctat ttctccatag cctaaaaatt
     4801 caagtgcagc agaaacgttg atactttcaa gaggaagacc ataatagatc gatgttaaat
     4861 tagctccaac agcttcagaa taaataactt cattgatgcc tatggcactt tgagttacag
     4921 tagttgtagc gaggggattt cgtgagactt ccgaaatccc tgcaggcatc actatgcctg
     4981 cccccgcgat cgctgccgat cgcggcgaca ccgaagtgga taagaatttc atggtggttc
     5041 caccagggtc tacatcccag tattgcccag ccaaggaata agtagctaaa aaagaaaagg
     5101 ctagcaaagc attacttata cgcattagag ttcctcaaaa aaaactttat tcgaaataaa
     5161 aaagcgagcg tcatcgattg tataaacaag acgagacgaa aataattcga aattcatttt
     5221 ttttattact gactttttag aaaaggtcat tattaaaaac ctagttttcc attagcattt
     5281 acccattctg acattagcgc taagcatttt gctaataatt ctttggatcg ttctgcagaa
     5341 gccgtctcca catagcatct aaactctgga gcattaccac ttggacgcaa atgaatcacc
     5401 tcttctgaat caaaaaccat acgataacca tcggtttcat ctatagaaat taacttgcct
     5461 gagaattttc caaacaaaga ttcgcc
//
LOCUS       DAOOBB010000135         5483 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_17068_length_5483_cov_4.007185, whole genome shotgun sequence.
ACCESSION   DAOOBB010000135 DAOOBB010000000
VERSION     DAOOBB010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 5483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5483
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17068_length_5483_cov_4.007185"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PLT31_06755"
     CDS             complement(<1..489)
                     /locus_tag="PLT31_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94872.1"
                     /translation="MIKKYGIFGFSALALLAIGCGDSSTGSGTTLQTYPSEINEEAKT
                     ISITLPSCEQISETEVRFDPEGNNATLEYDLSGGSLFIDDGFDVTEYTGNNSSLYGTW
                     TMADEECEENGLCETLKITSTSVSRSVDMSGICAIDYMVASMFADESLDGEAEIPEYT
                     KTS"
     gene            complement(823..1932)
                     /locus_tag="PLT31_06760"
     CDS             complement(823..1932)
                     /locus_tag="PLT31_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HPW94873.1"
                     /translation="MKITKVRFILFCIFALHIGSIFALPPQTWDAIFDAQGQGDYVSA
                     KIDGSIRFTKFVHYPPSQTLSFKVNDGTFQFAVTGEIVVTADEVEAGLYAQCNETKEV
                     WISCFNQPKVFKKKELVVDIKGVDLACGNDLYAIKKLKIKFPNKEAQEWWLRKFEKAE
                     KHKRERLSEFRIQEQEHRARVKAASSEFKDPRDGKVYRIIEIEGRRWFAQNVNFEVEG
                     ESYCYDDKDSYCDRGGRLYTLEGARQACPKGWHLPRDREWQDMITALTKCYEGVQNCG
                     NFGTKLKSKTGWQGGGGTDEYGFTVFSSGYRAPLGKKSFRYTDMGEYAGFWSSQNGRN
                     ETIWLWALGRMSETMVRQLAPSKYHAYSVRCIEGD"
     gene            complement(1929..2942)
                     /gene="hemB"
                     /locus_tag="PLT31_06765"
     CDS             complement(1929..2942)
                     /gene="hemB"
                     /locus_tag="PLT31_06765"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HPW94874.1"
                     /translation="MNSSLYPNPLLTRLRRNRKNASVRALVSETTLSASDLVYPVFLM
                     EGKNTEEPIHSMPGISRKTIDLFLEELKEIVNLGIRAIAPFPVVPESKKDSKATGSYD
                     PEGLIPECIRQIKEAFPELLIMTDVALDPYNTDGHDGLVSDSGEILNDQTVEVLCKMA
                     LCHAKAGADFVCPSDMMDGRVGAIRMALDQEEYTSTSIMAYSAKYASAFYGPFRDALH
                     SAPKAGDKKTYQMDPANKLEALREVEADISEGADIVMVKPAGPYLDIISEVHSRFPIP
                     VAAYQVSGEYSMIVSAAQNGFIDRSSTILESLMGIKRAGASLIWTYFAKEAAMLLNNK
                     GNQ"
     gene            complement(3022..3741)
                     /locus_tag="PLT31_06770"
     CDS             complement(3022..3741)
                     /locus_tag="PLT31_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPW94875.1"
                     /translation="MDTFIFIPAYNVENELPSLLSRIPEEIWDRASVSLINDGSKDDT
                     LNKAMEFKEKTNKKLEVFSFSENQGYGAVIKKGLSLALETDASFICCLHGDAQYPPEK
                     LPAFLEAMKKNSIDVLQGSRHVEKGGAKRGGMPFYKRCGGFFLTSIENLVFSTKLTDR
                     HSGFLCFSRRFLKEIPWSRLGHSFDIDLELLAFADARRYKVEERAIETRYAGEKSNLN
                     VITYGMRVLRIIYRKWRGFYS"
     gene            complement(3746..4789)
                     /locus_tag="PLT31_06775"
     CDS             complement(3746..4789)
                     /locus_tag="PLT31_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPW94876.1"
                     /translation="MKYSFKDPTVSIAIVGAGGFIGSHLLDALIERTSWRILAVDLDF
                     YRLQHRLHHPRIEFLCDDLKKESVVSRIASYPIVINLAAICVPSRYMREAKEVIESNY
                     NHPVALALACAKSGAWLIHFSTSEVYGKTSIEPCLLEEDSSPMILGSVRASRWSYACA
                     KQLTERYIASLQGLSWTVLRPFNFVGPRMDFIPGIDGEGIPRVLANFCAALLKNEPLF
                     LVDQGVSRRSFTSIHDAIHFIFAVLVHQEESLGQAFNIGNPENELSIAELADRMRQIY
                     ASLKQVSIDQVSPVQLISSKEYYGEGYEDSLRRIPSIEKAKRLLGYVPNEALDVTLQE
                     TLSWFIEHYRLAL"
     gene            complement(4795..5235)
                     /locus_tag="PLT31_06780"
     CDS             complement(4795..5235)
                     /locus_tag="PLT31_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94877.1"
                     /translation="MNTKLKWFSLFLMASFIVACDSGSSSDTDKEEVEDCSIDTDGTV
                     KVFSPKGGEVFHIGDSITVKWGAKYADGTGFRILYYFDEESQALDLTEESVAPEAPKG
                     DQCYSVQVVLDGDDFPASDAAFITVRDYSNGSVSGKSKSFQVVE"
     gene            complement(5255..>5483)
                     /locus_tag="PLT31_06785"
     CDS             complement(5255..>5483)
                     /locus_tag="PLT31_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94878.1"
                     /translation="PDHQAWSSSIVYGLEFSKMDYPFFIALSIPIHDKAGSANPSDEY
                     DESPMREWNLPDFDDLLQQWTLGVGIKGSFF"
BASE COUNT         1775 a         1122 c          943 g         1643 t
ORIGIN      
        1 actggtttta gtatattctg gtatttctgc ttcaccatca agagactcgt ctgcaaacat
       61 agaagcgacc atataatcaa tagcgcatat accagacata tccacagatc ttgatactga
      121 agtagaagtg atttttaatg tttcacagag cccattctct tcacattctt catcagccat
      181 ggtccaagtt ccataaagtg aagaattgtt tcctgtatac tccgttacat caaatccgtc
      241 gtctataaat agagaaccac ccgataaatc gtattccaaa gtagcattat tcccttctgg
      301 gtcaaagcga acttctgttt cagaaatttg ttcacaagaa ggaagtgtta tagaaatagt
      361 ttttgcttct tcattaatct ctgaaggata ggtttgaaga gtagttccag agccagtact
      421 actatcacca cagccaatgg caagtaaagc taatgctgag aaaccgaaaa taccatattt
      481 tttaatcatt taataactcc ttattaggtt tatatttaaa gtaacttaaa aacaatcata
      541 atgtaaactt tttttaataa atagaaataa atatcacaca aaaaattgtg taattcctta
      601 taatcatttt aatgttaaaa ttcataaaag ggattatgaa ataccttaaa tactttttta
      661 tgttttggga ataaaaaaca aaaaagggat tacgaattgt tttgatgaat taatattcaa
      721 caaagtgttt caggctttta tttaacgatt gattgtaaaa attttataaa gcattaatat
      781 ttttgaaatg tacacaacaa taaattgtgg tttcaaatag ccttaatcgc cttcaataca
      841 acgcactgaa taagcgtggt atttacttgg agctaattgg cgaaccatgg tttctgacat
      901 acggcctaaa gcccaaagcc atattgtttc attgcggcca ttttgagaag accaaaaacc
      961 agcatattct cccatgtcgg tataacggaa agattttttc cctaaaggag cacgataccc
     1021 tgaggagaaa acggtaaagc cgtattcatc agttcctcca ccgccttgcc aacctgtttt
     1081 tgattttaat ttagttccaa aattaccaca attttgaaca ccttcataac atttggtgag
     1141 agccgtaatc atatcttgcc attcacggtc tctaggcaaa tgccatccct ttgggcaagc
     1201 ttggcgagca ccctctaaag tgtataatcg tcctccacga tcgcaataag agtctttatc
     1261 atcataacaa taagactcgc cttctacttc aaaatttaca ttttgagcaa accaacgacg
     1321 tccttcaatt tcaataattc tatatacttt tccatctcta gggtccttaa attcactaga
     1381 agccgctttt accctagctc tatgttcttg ttcttgaata cggaattcag ataagcgttc
     1441 tcttttatgt ttttctgctt tttcaaattt tctaagccac cattcttgtg cctctttatt
     1501 tggaaatttg atttttaatt tttttattgc ataaagatca tttccacaag ctaaatcaac
     1561 tccttttata tctacgacta attctttttt cttgaacact ttaggttgat taaaacacga
     1621 tatccaaact tccttggtct cattacattg tgcgtataga ccagcttcca cttcatctgc
     1681 cgtgaccaca atttctcctg tcacagcaaa ctggaatgta ccatcattga ctttaaatga
     1741 tagtgtttgt gaaggagggt aatgtacaaa tttggtaaag cgaatagaac catctatttt
     1801 tgcagagaca taatcccctt gcccttgagc atcaaatatg gcatcccatg tttgtggagg
     1861 aagtgcaaaa atagaaccta tgtgtaaagc aaatatacag aatagaataa aacgaacttt
     1921 agttattttc attggttccc cttattattt aatagcatcg ccgcttcttt tgcaaaataa
     1981 gtccagatca aagaagcccc agcacgctta atacccatta gactttcaag aatagttgaa
     2041 gatcgatcaa taaaaccatt ctgtgcagcc gatacaatca tagaatactc gccgctcact
     2101 tgatatgccg ctacaggaat tggaaaacga gaatgtactt cggaaataat atcaagatat
     2161 ggccccgcag gctttaccat cacgatatct gcgccctctg aaatatcagc ttcaacttct
     2221 cgtaatgctt ctaatttatt ggctggatcc atttgatacg ttttcttgtc gcctgctttt
     2281 ggtgctgagt gaagggcgtc tcggaatggt ccataaaaag ccgaagcata ttttgcactg
     2341 taagccataa tggaagtaga agtatattct tcttgatcca aagccatacg aatggctcca
     2401 acccgaccat ccatcatatc ggaagggcaa acaaaatcag ctcccgcttt tgcatggcaa
     2461 agagccattt tacagagaac ctcaactgtt tgatcattta agatttctcc agaatcgctt
     2521 acaagtccat catgaccgtc agtattgtaa ggatctaaag caacatctgt cattattaaa
     2581 agttctggga aagcctcttt gatttgacgg atacactcag gaattaaacc ttctggatca
     2641 taagatcctg ttgcttttga atcttttttg gattcaggga ctacaggaaa aggggcaatg
     2701 gctcgaatac ccaagttcac aatttctttt aattcttcta aaaataaatc gatggtcttg
     2761 cgggaaattc caggcatcga atgaatcggt tcttctgtat ttttcccttc catgagaaat
     2821 acaggataca ctaaatcaga agcagaaaga gtggtttctg aaactaacgc cctaacggaa
     2881 gcgtttttac gattgcgacg aagtctcgtc aaaagaggat tagggtaaag agacgaattc
     2941 atagattaaa ttttagaaaa aattactttt tagagtttat aaacagcatt aaggcaacct
     3001 caaaaatgcg acattctgcc tttaagaata aaatcctctc cattttcgat aaataattcg
     3061 caaaacgcgc attccataag taatcacatt gagatttgac ttttctccag cataacgcgt
     3121 ttcaatcgcc cgttcctcta ctttgtaacg ccttgcatct gcaaaagcta atagttcaag
     3181 gtcgatatca aaagaatgcc ccaatcgaga ccaagggatt tcttttaaga aacgcctaga
     3241 aaaacataaa aagccactat gacggtctgt gagctttgtc gaaaagacaa gattttcaat
     3301 cgaggttaaa aagaagcctc cacaacgttt ataaaaaggc attcctccac gtttagctcc
     3361 ccccttttct acatgcctag aaccttgaag aacatcaata gaattttttt tcatcgcttc
     3421 taaaaaagcg ggcagctttt ctggagggta ttgagcgtct ccatgaaggc aacaaataaa
     3481 agacgcatcc gtttctaaag ctaaggaaag cccttttttg atcacggctc cgtacccttg
     3541 attttcagaa aaagaaaaaa cttccaattt tttatttgtc ttttctttaa attccattgc
     3601 cttatttaag gtatcatctt ttgagccatc attaataagg gataccgaag ctcgatccca
     3661 aatttcttct ggaattcgag agagtaaaga gggtaattcg ttttcaacat tataggctgg
     3721 aataaaaatg aaggtgtcca taaaatcaca atgccaaacg gtagtgttct ataaaccaag
     3781 agagtgtttc ttgaagagtc acatctaaag cttcatttgg gacatatccc aataaacgtt
     3841 ttgctttttc aatagaagga attcgtctta aagaatcttc gtaaccttcg ccataatatt
     3901 cttttgaaga aatcaattgc acgggcgaga cttggtctat ggagacttgc tttaatgaag
     3961 cataaatctg acgcatgcga tccgctaatt cagcaatcga tagttcattc tcaggattcc
     4021 caatattgaa ggcttgccca agcgattctt cttgatgaac taagactgca aaaataaaat
     4081 ggatggcatc gtgaatcgat gtaaaagatc gccttgaaac accttgatcc actaaaaata
     4141 gaggctcatt ttttagaaga gccgcacaga aattagctaa cactctaggg atcccttctc
     4201 catctattcc agggataaaa tccatacgag ggccaacgaa attaaaaggg cgaagcacag
     4261 tccaagacaa gccttgtagt gaggcaatat agcgttctgt aagctgtttt gcacaagcgt
     4321 aagaccagcg agaagcacgc acagaaccaa gaatcatagg agaagaatct tcttctagta
     4381 aacagggttc tatggatgtt tttccataaa cttctgacgt ggaaaaatga atcaaccaag
     4441 ccccagactt ggcacaggct aatgcaagtg ccactgggtg gttgtaatta ctttcaatga
     4501 cttcttttgc ctctcgcata tagcgactag gcacacaaat tgccgcgaga ttaatcacaa
     4561 tcggatacga tgcaattcga gaaaccactg attctttttt tagatcatca cataagaatt
     4621 cgatcctagg atgatgaaga cgatgttgta atcgataaaa atctaaatct acggccaaga
     4681 tacgccacga ggttctctca atcaacgcat ccaataaatg cgaaccaata aatccgcccg
     4741 caccaacaat cgctatcgat accgtaggat ctttaaacga atatttcaat ggaattactc
     4801 aacgacttgg aaagattttg atttaccaga cacagaacca ttgctataat cacgaactgt
     4861 gatgaaagca gcgtctgaag cagggaaatc atctccatca agaacgactt gtactgaata
     4921 acattgatcg cctttagggg cttcaggggc aacgctttct tctgttaaat cgagtgcttg
     4981 agattcttcg tcaaaataat aaaggattct aaagcctgta ccatcagcat atttggcacc
     5041 ccattttact gtaatagaat cacctatatg gaacacttct ccaccttttg gagaaaagac
     5101 tttcacagtt ccgtccgtat caatagagca atcttcaact tcttctttat ctgtatctga
     5161 acttgaacca ctatcacaag cgactatgaa agaagccatc aaaaaaagcg aaaaccattt
     5221 taattttgta ttcataattt atacctctgt taatttaaaa gaaagagcct ttgatgccaa
     5281 cgcccaaagt ccattgttgt aaaagatcat caaagtctgg tagattccat tcacgcatag
     5341 gagattcatc gtattcatca cttggatttg cagaccctgc tttatcgtga atagggatcg
     5401 aaagagcgat aaagaatggg taatccattt tagaaaattc taagccataa acaatagaag
     5461 aagaccatgc ttgatgatca gga
//
LOCUS       DAOOBB010000136         5472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_17097_length_5472_cov_4.060366, whole genome shotgun sequence.
ACCESSION   DAOOBB010000136 DAOOBB010000000
VERSION     DAOOBB010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5472
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17097_length_5472_cov_4.060366"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PLT31_06790"
     CDS             <1..256
                     /locus_tag="PLT31_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94879.1"
                     /translation="YYLNGYQTGFLRNDDGTILKESLSYGKIMAGINFQSENKLRSFT
                     VTPGFWFRPKKGQYNGTLLQQFLIHLQYQGHFSFPFRSRK"
     gene            complement(185..940)
                     /locus_tag="PLT31_06795"
     CDS             complement(185..940)
                     /locus_tag="PLT31_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94880.1"
                     /translation="MRFFSLFLLFVFMGQIFAQDICYQSLVEKSFLINFEGRFINSEK
                     SRSHIDVSWRHHEQAPDSFYVYLSGKRSFLFISTNDYRVLWIQPENVKRQMATHHLKE
                     KIGNTPIHWDDLDLLANGSFFCQDSSAKANFIFATAYSQTWYSLVLNSETSPTKIRMR
                     GNRGLSRDVTIHSWKNFNDALLPAILDINSPESTGSLWIRSAYPIESKIPMDPLLKNV
                     QSKNGRINSLLLPRTERKRKMPLILQMDKELLQ"
     gene            complement(1060..2151)
                     /gene="recA"
                     /locus_tag="PLT31_06800"
     CDS             complement(1060..2151)
                     /gene="recA"
                     /locus_tag="PLT31_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPW94881.1"
                     /translation="MAKKTAAEATSPKNERAKAVEAAIAQIEKNYGKGSIMTLGSQPI
                     QDIPVIPSGCIQLDMALGVGGFPRGRIIEIYGPESSGKTTLTLHAIAEAQKLGGVAAF
                     IDAEHAFDATYARKLGVKIEELLVSQPDTGEQALDIAETLVRSGAVDIIVVDSVAALV
                     PQAEINGEMGDSHMGLQARLMSQALRKLTGILSKSNTCLLFINQLRMKIGVMFGNPET
                     TTGGNALKFYATQRIDIRRIAAIKNGEDVIGNRTRIKVVKNKVAAPFLQCEFDILYGQ
                     GISREASILDLAVELELIEKSGSWFSYNSERIGQGRENARLFLKNNPDITAKLEIQIR
                     ENMKDVQLFSLGADDALSVESENSSSTEE"
     gene            complement(2210..3475)
                     /locus_tag="PLT31_06805"
     CDS             complement(2210..3475)
                     /locus_tag="PLT31_06805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HPW94882.1"
                     /translation="MSTPEVALISIGTELTQGFTLNTNAHWLSNECRQLGFKIAYHLA
                     LPDDASYWNKTFKMLKEANVSIVIITGGLGPTADDKTRELIAETFGKPLVYNVNCEEK
                     IKAWFQKRNLTYIETNHIQACFPEGSIILENPVGSAPGFQIDEGDLHLFCLPGVPSEM
                     KKMFQVHLVPWLQSISTTTWYHQDLRLFGLSESALEALLRTFEFENGVYWSSLPMIDC
                     LLFRLYTSKSKEALTKARDQFVNSLGDKADQILISDNGENLIQVIMTLLKEKNQFLGV
                     AESCTGGLLASEIVKESGSSLVFKGGVVAYQNEIKTKILHVPEEILKTEGAVSEATVL
                     SMAEHTLNVLNCDWGIATSGIAGPTGGTKEKPVGLVWMAIASKQQKIAFSEIFLGNRG
                     EIQMKSVYKVLNRLRLSILEQKNTCSKVQ"
     gene            3618..4589
                     /locus_tag="PLT31_06810"
     CDS             3618..4589
                     /locus_tag="PLT31_06810"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HPW94883.1"
                     /translation="MDLILKWLVAVFLSLSMFIGLAFADDSPLVRGKTGIEALDTLAT
                     YEWDVPTKRNSLPLTLLSGILPGGAQIYSEHYIRGGFLVGIEIALVYEVFINKSFQQD
                     RRFKLARPLQDSVAFYNNLIRNTSDIDTISSLHLKRLGFINNIKRISDKKMEEEDLRK
                     AEFAWLVGLHLYGFVDGFGIWWNNQHRNMELKSSKKAVLLALIPGLGQMYNEEFGKAG
                     LLYMGLTGAFASIHTSQKVVEYYLERRRIITAEAASSTEIEYLTERITYYRKNRNQYI
                     WGAALIYLYSIGDAFVDAMMSDFDNPVHFAILPNFKGGLQAAFLLDF"
     gene            complement(4603..5217)
                     /locus_tag="PLT31_06815"
     CDS             complement(4603..5217)
                     /locus_tag="PLT31_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPW94884.1"
                     /translation="MILWTIRHTKPLNINQVCYGRTDFDVSPSFETEYPPALEALKTA
                     KINRLFSSPLLRCKRLAEKVSEHLSIPFEINEDIIELFFGHWENVRLVDVPSHEMDSW
                     KADLRGYRFPGNGESFRDVDIRVSKAIESSMDMGEIAWVTHAGVIASLQHTFCGVPEN
                     DFIEGKFGYSMVTRFEFEADKNGRIQGRFDTIHQGLPQPPLGVD"
     gene            complement(5284..5381)
                     /gene="ffs"
                     /locus_tag="PLT31_06820"
     ncRNA           complement(5284..5381)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLT31_06820"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
BASE COUNT         1639 a          973 c         1065 g         1795 t
ORIGIN      
        1 ctattatttg aatggctatc aaactggttt tttaagaaat gatgatggaa ctattttaaa
       61 agaatcattg agttatggta aaatcatggc aggaataaat ttccaatcag aaaataaact
      121 tcgctctttt actgtgactc ctggattttg gttccgccct aaaaaagggc aatataatgg
      181 aactttattg cagcagttcc ttatccattt gcaatatcaa gggcattttt cttttccttt
      241 ccgttctagg aagtaaaaga gaatttattc ttccgttttt agattgaaca ttttttagta
      301 agggatccat cggtatcttg gattcaattg gatatgcaga acgaatccat aaagagcctg
      361 tactttcagg actattaata tctaagattg ctggcaataa ggcatcatta aagtttttcc
      421 aagaatgaat ggtaacgtct cgagataagc ctctgtttcc gcgcattctg atctttgttg
      481 gtgacgtttc gctattgaga actaaagaat accacgtctg agagtaggcc gttgcaaaaa
      541 taaaattagc cttggcactt gagtcttgac aaaagaaaga accattcgct agtagatcta
      601 aatcatccca atgaatagga gtgttcccta ttttttcttt tagatgatga gtagccattt
      661 ggcgctttac gttttctggt tgaatccata ggactcggta gtcattagta gaaataaata
      721 gaaatgatct tttaccagaa agataaacat aaaaggagtc tggggcttgt tcgtgatgtc
      781 tccaagaaac atctatatgg gatcggcttt tttcactatt aataaaacgt ccctcgaaat
      841 taattagaaa agatttttct actagtgatt gataacaaat atcttgagca aagatttgtc
      901 ccataaaaac aaaaagcaag aataaagaaa aaaagcgcat agttaccaat ttatataata
      961 gacttcgtct taataataat aaaaatagta actatggctt ataggaatca ctcatggaag
     1021 ttggtacatg gctaaaccaa acttttgaga tatcccgttt tattcttctg tacttgaaga
     1081 gttttcggat tcaacagaaa gagcgtcatc tgctccaaga ctaaataatt gaacatcctt
     1141 catattttcg cgaatttgga tttctaattt tgccgtaata tcaggattgt tctttaggaa
     1201 taatcgagca ttttctctac cttggccgat gcgttcactg ttgtaagaga accaagagcc
     1261 acttttttct atcagttcaa gttcaaccgc aagatcaagt atagatgctt ctcttgaaat
     1321 accttggcca tatagaatat caaattcaca ttgtaagaat ggagcggcta ctttgttttt
     1381 taccacttta attctggttc tatttccaat gacgtcttcg ccatttttaa ttgctgcgat
     1441 acggcgaata tcaatgcgtt gagtcgcata gaatttaaga gcattaccac cagtagttgt
     1501 ttctgggtta ccaaacataa caccgatttt cattctaagc tgattaatga agagtagaca
     1561 agtgttagat ttagataaga tccccgtgag ctttctgagt gcttgagaca taagccttgc
     1621 ttgtaagccc atgtggctat cgcccatttc accattgatt tctgcttgag gtacaagagc
     1681 tgcgacggag tctacaacaa taatatcaac tgctcctgaa cgaaccagag tttctgctat
     1741 atctaaagct tgttctcctg tatctggttg agaaacgaga agctcctcga ttttgacgcc
     1801 taatttacga gcgtaagtgg catcaaaagc atgttctgca tcaataaaag cagcgacgcc
     1861 tccaagtttt tgagcttcag caatagcatg aagagtgagt gttgtttttc cagaagattc
     1921 gggcccgtag atttcaataa tacgacctct tggaaaaccc cctacaccta gagccatatc
     1981 taactgaatg cagccgcttg gaattacggg aatatcttga ataggctgac ttcctaaagt
     2041 cataatagag ccttttccgt aatttttttc aatttgtgca attgcagcct ctacggcttt
     2101 tgccctttcg ttcttaggtg aggttgcttc agctgctgtt ttctttgcca tatttttctc
     2161 cgattgttct tttgctctga ttaggagcga ttgtttatta atataataat tactgcactt
     2221 tcgagcaagt gtttttttgt tcaagtattg aaagtcgtaa acgattcaat actttataaa
     2281 cgcttttcat ttggatttct ccgcggtttc ctaaaaaaat ttcagaaaaa gcaatttttt
     2341 gttgttttga ggcaatggcc atccaaacta agcctacagg tttctctttt gtgcctccag
     2401 ttggccctgc aattccagaa gtggcgattc cccagtcaca atttaagacg ttaagagtgt
     2461 gctctgccat agataaaaca gtggcttcac tgacggcgcc ttctgtttta aggatttctt
     2521 ctgggacatg aagtattttt gtttttattt cattttgata agcgaccact cctcccttaa
     2581 aaacaagaga agatccagat tctttcacta tttcagaggc aagtaagcca ccagtacagg
     2641 attctgcaac tccaagaaat tgatttttct cttttaaaag agtcataatg acttgaatta
     2701 aattttctcc attatcagaa ataagaattt ggtctgcttt gtctccgaga gaattcacaa
     2761 attggtctct tgctttggta agagcttctt ttgatttaga tgtgtaaagg cgaaatagta
     2821 agcagtctat cataggaagg ctactccaat aaacgccatt ttcaaattca aatgtcctaa
     2881 gtagtgcttc caaagccgat tcagaaagcc caaataagcg taaatcttga tgataccaag
     2941 ttgtggtact tatagattgg agccaaggga ctaaatgaac ttgaaacatt tttttcattt
     3001 cagatgggac tcctggtaaa cagaataaat gcaggtctcc ttcgtctatt tgaaaacctg
     3061 gagcacttcc gactggattt tctaaaatga tcgatccttc tgggaaacaa gcttgaatat
     3121 gattcgtttc tatataggtt aagttccttt tttgaaacca agctttaatt ttttcttcac
     3181 aattaacgtt ataaactagc ggttttccaa atgtttcagc gatcaattct cgtgttttat
     3241 cgtctgcagt ggggccaagt ccgccagtaa taatgactat ggaaacgttt gcttctttta
     3301 acattttaaa tgtcttattc caataagagg catcatctgg tagagctagg tgataagcga
     3361 tcttaaaacc taattgacgg cattcattgg atagccagtg agcgtttgta tttaaggtga
     3421 acccttgggt gagttctgta ccgatagaga tgagtgcgac ttctggtgtt gacattgtat
     3481 ttccttattt ctaaaactag aatagatttt tgacagtttt tgtcaaaacg aagaaaaacg
     3541 ccattttcga gagaaagggc ttgtaaaaga taggtaagca tgattaaacg gactttattt
     3601 tttatcttta tcttccaatg gatttaattc ttaaatggct tgttgctgtt tttttgtctt
     3661 tatcgatgtt tatcggtctg gcatttgcag atgattctcc tctagttcga gggaaaacag
     3721 ggatagaggc tttagatact ttagcaactt atgaatggga cgttcctact aagcgtaact
     3781 ctcttccctt aacgttgctt tcgggaattt tacctggtgg agctcagatt tattcggagc
     3841 attatattcg cggaggcttt ttagttggga ttgaaattgc cttagtctat gaagtgttta
     3901 ttaataaatc ttttcagcaa gatagacgat ttaagctagc tcgtccgtta caagattctg
     3961 ttgcttttta taataatcta attcgaaata cgtctgatat agatacgata tcttctttac
     4021 acttgaagcg attaggcttt atcaataata tcaagcgaat cagtgataaa aaaatggaag
     4081 aggaagattt gcgtaaagca gagttcgcgt ggcttgtagg cttacacctc tatggttttg
     4141 tggatggatt tggtatttgg tggaataacc aacaccgaaa tatggaatta aagtcttcaa
     4201 agaaagcggt cttacttgct ttgatccctg gtctaggtca aatgtataat gaggagtttg
     4261 gcaaagcggg tttgctttat atgggtttaa caggagcttt cgcgagtatt cacacaagtc
     4321 aaaaagtggt ggagtattat ttagaaagac gaagaatcat aactgctgaa gcagcgtctt
     4381 ctaccgagat agaatacctg acagagagaa ttacgtatta tcgtaaaaat cgaaatcagt
     4441 atatttgggg agcagcgtta atttatctgt attctattgg cgatgctttt gtagatgcta
     4501 tgatgagcga ttttgataac cccgttcatt ttgccatttt accaaatttt aagggtggct
     4561 tgcaagcggc ttttttattg gacttttaag ggaaccttct aattaatcaa cgcctaaagg
     4621 gggttgaggt aatccttgat gaatggtatc aaaacgtcct tgaatacggc cattcttgtc
     4681 tgcttcaaat tcaaaacgag ttaccataga atacccgaat ttcccttcta tgaagtcatt
     4741 ttctggaaca ccacaaaagg tatgttgtaa agaagcaatg acaccagcgt gagtcaccca
     4801 ggcaatttct cccatatcca tagaactttc gatggctttt gaaacacgta tatctacatc
     4861 tcgaaatgat tcgccattcc ctgggaaacg ataacctctt agatctgctt tccatgagtc
     4921 catttcatgg cttggaacat ctacgagccg tacattttcc cagtggccaa aaaagagttc
     4981 gataatatct tcattaattt caaaagggat acttaaatgt tcgctgactt tttctgcaag
     5041 tcgcttgcac cttagaaggg ggcttgagaa aagtcgattg atttttgctg tttttagtgc
     5101 ttctaatgca ggggggtatt cagtttcaaa agagggagat acatcgaaat cagttctacc
     5161 ataacaaact tgattaatat taagaggctt tgtgtgtcta atagtccata aaatcataaa
     5221 aacagcataa aaaaagacgt taaatacgcc taagtagaaa aaaaggggtg aggaatgtaa
     5281 aaaaagtcct agactacctg cagcacataa aaattcacaa gtccgttgct gctttcgccc
     5341 tggcgagttg cccgcaaata gtcattgcgc agggtctagg aaaaaagaat atagcaatat
     5401 aattcttttt ttcatttttt atttgtaaaa aaaaatgagg atataaaaat agaaccgttt
     5461 gtagtaaaaa aa
//
LOCUS       DAOOBB010000137         5467 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_17121_length_5467_cov_4.458610, whole genome shotgun sequence.
ACCESSION   DAOOBB010000137 DAOOBB010000000
VERSION     DAOOBB010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 5467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5467
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17121_length_5467_cov_4.458610"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1533
                     /locus_tag="PLT31_06825"
     CDS             <1..1533
                     /locus_tag="PLT31_06825"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPW94885.1"
                     /translation="LQEAAVSLTIPLISKTRFASSLAYRYSDNDYEFLNRNGTEYDTD
                     DDFLVKRKNAWYLQFSGNHTFYHTHENGGFSTLSFRHEKEKGGNPGKESNQTEVAGFN
                     RDLGQVFYSYEPIPYKNLQFTMSLSGQIEKSVSHSYYPLDKIGFPNTYYMEYGAIRYF
                     VQPKISVELPADSSRSWAASLYLASSAERLEDRDNNEWISDYPWHLEQYAVEGASNIQ
                     WFLSRIISLELENAVKVFYNENSGGQWSLPVRNDSLISGSNQDLLGSGRLAMHFGHLK
                     SPWRASLSLGRYFREPELMEQYGVYRNVLPNPDLKEETGWMGEISLSWKSASEKTKIL
                     STYFETHQKNGIIWVTNNSFSKPFNISKAITRGVELSLESKPTSFFKTRLDATIQKAL
                     DDSSDPLYQGKKLPEEPTTTYHAEAAWLLPYHLEWSFQAGYRSTLYKDRANRQKIPAQ
                     SLYHSYLTWNPLKRTKFVLSVQNITNTEYQSIYSSYPLPGRTFSLTFIQQIGINKDEN
                     NQ"
     gene            1517..2644
                     /locus_tag="PLT31_06830"
     CDS             1517..2644
                     /locus_tag="PLT31_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94886.1"
                     /translation="MKTINNTTIFLGIIISIFIVACSEDSPTTPKDEIESKTLGLVFT
                     SDYVNGELRWFDIEADSLSPNKITLAQDSRVIVQGSQVFVLERFGADNLVRLDPSKLD
                     QEDASVLYQTSLEDGSNPSDLIALDSQTAWLGLEGSAHLLKINTAVSAKGKTLKTVDL
                     SKYTVSGNISPNLIKLSLKGDTLLALLQRLDMYQAKLPGLIVLLDANEGTILDTIPLK
                     TNNPTSFAWIENVLWVSTQGVYNDVYGCNSDSLRGIESIDFEKKKSTVKFFGKNLGGG
                     VSSFTVDQKNKKIYASVLGVDGGYSLAKVDIEKNKISLLDEIEDASGSLYWDANSDLL
                     FVGDRSFENAKLWGYDGSSFFEIKGKDVLPPYNMAVISYEE"
     gene            complement(2719..4179)
                     /gene="ahcY"
                     /locus_tag="PLT31_06835"
     CDS             complement(2719..4179)
                     /gene="ahcY"
                     /locus_tag="PLT31_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HPW94887.1"
                     /translation="MDYKVKDINLASWGRKEIELAETEMPGLMALRREYGAQKPLTGA
                     RIMGSLHMTIQTAVLIETLTELGADVRWVSCNIFSTQDHAAAAVVVGKNGSVDAPKGT
                     PVFAWKGESLEEYWDNTAKALAWPDGGTANLILDDGGDATMLVVRGAEFEKAKEVPAF
                     NPETDSEEWGVFLELCRQIYAQDPSRWTRTSKAICGVSEETTTGVHRLYDLAKQGRLP
                     FAAINVNDSVTKSKFDNLYGCRHSLIDGINRASDVMIAGKVAVVCGYGDVGKGCAQSL
                     RGQGARVIITEIDPICALQAAMEGYEVKTLDEVVSYADIFVTTTGNLKIISAKQMSLM
                     KHRAIVGNIGHFDNEIDMAGLKKVPGIVRTEIKPQYDEWTFADGHSILILAEGRLLNL
                     GCATGHPSFVMSASFTNQTIAQIDLWLNNQGKETVAGVKYEKGKVYTLPKILDEKVAR
                     LHLDKLGVHLTTLTQEQADYLGVAVEGPYKVDFYRY"
     gene            4514..>5467
                     /locus_tag="PLT31_06840"
     CDS             4514..>5467
                     /locus_tag="PLT31_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94888.1"
                     /translation="MSFKKIGLVLLGVFFLAACSGENGVDGRDGESVNVDSLAAVLRG
                     ELSQDLWDSMHSETVLDSVYDTLFNDAFTDVWLDSARNALLDSLLDVSYDSLYSVLYD
                     SVYIDLYDSVYKDIYEKSVTKDLFSYINFKKENFNTAFANQYSLMYNGFEHPVPLGVK
                     IENSGNLWHTVIVKAWIPDYSDTGIVSGIVNPNKSNVFAPEIKLIPEKYLHLKAAAPV
                     LVQVRAFALENNREILIFSESYNAEILPVELFGSEYVGVDISPWWNVWVTPNMDSITS
                     IHSELASLMPSGYGGYQLYGFSTMEESVNNQVKKVYEVLQKK"
BASE COUNT         1764 a          988 c         1020 g         1695 t
ORIGIN      
        1 cttcaagaag cggcagtgtc tctaaccatt cctttaattt ctaaaacacg ctttgcctct
       61 agtttagctt atcgatattc agataatgat tacgaattct taaatagaaa cggaacggaa
      121 tatgatacag acgatgattt tttagtgaaa agaaaaaatg catggtactt gcagttttca
      181 gggaatcata ctttttatca tactcacgag aatggaggat tctccacttt aagttttagg
      241 catgaaaaag aaaagggtgg taatccaggg aaagaatcga atcaaacaga agtggctggg
      301 tttaatcgag atttaggcca ggttttttat tcttatgagc ctattcctta taaaaaccta
      361 cagttcacaa tgtctctttc agggcaaata gaaaagtctg tttctcattc ctattaccca
      421 ctagataaaa tcggtttccc taatacatat tatatggaat atggtgctat acgttatttt
      481 gttcaaccaa aaataagtgt agaattacca gcggattcgt ctcgttcttg ggcagcttct
      541 ctatatcttg cctcttcagc agaacgttta gaagatcgag ataataatga atggatttca
      601 gattatcctt ggcatttaga acaatatgct gtagaaggtg catccaatat ccaatggttt
      661 ttatcgcgta ttatttcttt agaattagaa aatgccgtta aagtttttta taatgaaaat
      721 tcaggcggac agtggtcttt acctgttcga aacgatagct tgatcagtgg atccaatcaa
      781 gatttgttag gctctggaag attagcaatg cattttggac atctcaagtc accgtggagg
      841 gcttcgttat cattaggtcg ttattttaga gagccagagc ttatggagca atatggcgta
      901 tatcgaaatg ttcttccgaa tccagattta aaagaagaaa caggatggat gggagaaatc
      961 agtttatcat ggaaaagcgc ctctgagaaa acaaaaatac tgagtactta ttttgaaacg
     1021 catcaaaaaa atggaatcat ttgggttacc aataattcat ttagcaaacc attcaatata
     1081 tcgaaagcga ttacaagagg cgttgaactt tctttagaaa gcaaacccac ttcttttttc
     1141 aaaacaagat tagatgccac tattcaaaaa gctcttgatg attctagtga ccctttatat
     1201 caagggaaaa aacttccaga agaacccacc acaacatatc atgctgaagc tgcatggcta
     1261 ttaccatatc atttggagtg gtctttccaa gcgggatacc gtagcaccct ttataaagat
     1321 cgagcaaatc gacaaaaaat cccagcacaa tctctttatc acagttattt aacttggaat
     1381 cctttaaaaa gaaccaaatt cgttttatca gttcaaaata tcaccaacac agagtatcaa
     1441 agtatttact cctcttaccc ccttccagga agaacatttt cactcacatt tatacaacaa
     1501 ataggaatca acaaagatga aaacaatcaa taatactact atttttttgg gaataatcat
     1561 ttccatcttt atagtcgctt gtagcgaaga ttcgcctacg acgcctaaag acgaaataga
     1621 gagtaaaact ctagggctcg tttttacaag tgattacgtg aatggggaat taagatggtt
     1681 tgatattgag gcagactctt tatcaccaaa taaaataact cttgctcaag actctagagt
     1741 cattgtacaa ggctcacaag tttttgtttt agaacgtttc ggggcagata atttagtacg
     1801 cttagatcca tctaaattag atcaagaaga cgcttctgtt ctttaccaaa cttcgttaga
     1861 agacggctct aatccaagtg atcttatcgc attagattct caaacggctt ggctcggctt
     1921 agaagggagt gctcatctct taaaaataaa tacagctgtt tctgctaagg gaaaaacgct
     1981 taaaacggtg gatctatcca aatatacagt ctctggtaat atttctccaa atctcatcaa
     2041 attaagctta aaaggagata cattacttgc ccttttacaa cgactagaca tgtatcaagc
     2101 caaattacct ggtttaatcg tattattaga tgcaaatgag ggaactattt tagacaccat
     2161 tccattaaaa acgaataacc ccacctcatt tgcttggatt gagaatgttt tatgggtttc
     2221 cacacaaggc gtttataacg atgtctacgg ctgtaatagc gatagcttaa gaggaataga
     2281 atcgattgat tttgaaaaaa agaaatctac agtaaagttc tttggaaaaa atttaggtgg
     2341 aggagtttct tcttttacag ttgatcaaaa aaataaaaaa atatatgctt cagtattagg
     2401 ggtcgatgga ggctattctt tagcaaaagt cgatatagaa aaaaataaaa tcagcctatt
     2461 agatgaaata gaggacgcca gtggctcttt gtattgggat gcaaattcgg acttactttt
     2521 tgtaggagac cgaagctttg aaaatgcaaa attatggggt tatgatggat ccagtttctt
     2581 tgaaataaaa ggaaaggatg tgcttccccc ttataacatg gctgtaattt catatgaaga
     2641 ataagcatca atttatttaa aataaaaagc tccgaaagag attttctttt tcggagctaa
     2701 ataaaaaata tttgaagact aatagcgata gaagtccact ttataagggc cttcaacggc
     2761 aacgcctaaa taatcagctt gttcttgtgt taaagtagtt aaatgaacac cgagtttatc
     2821 aaggtgtaaa cgggccactt tttcatcaag aatcttaggt aaagtataca ctttaccttt
     2881 ttcatattta acaccagcta cagtttcttt cccttgatta ttcaaccaaa gatcaatttg
     2941 tgcaatagtt tggtttgtaa aactagcact catcacaaaa ctggggtgtc ccgtggcaca
     3001 acctaaattc agcaaacggc cttcagcaag aattaaaatg ctatggccat cagcaaatgt
     3061 ccattcatca tattggggct tgatttctgt gcgtacaatg ccaggcacct tctttaatcc
     3121 agccatgtcg atttcgttat caaaatgacc aatatttcca acgatagccc tgtgtttcat
     3181 taggctcatt tgttttgcag agataatctt taggttacca gtcgtggtga caaaaatatc
     3241 agcataagaa accacttcat ctaaagtctt tacttcataa ccttccatgg cggcttgcaa
     3301 cgcacaaata ggatcgattt ctgtgattat cactcgagcg ccttgcccac gtaaagattg
     3361 ggcacaacct ttacccacat cgccatagcc acagacaaca gctactttac ccgcaatcat
     3421 cacgtcgcta gcacggttaa ttccatcaat caaagagtgg cggcaaccat ataagttgtc
     3481 aaattttgat ttagtgacgg agtcgttcac attgatggca gcaaaaggta aacgcccttg
     3541 ttttgcaaga tcataaagac ggtgaacgcc tgtagttgtt tcttcagaaa cgccacaaat
     3601 cgctttagaa gtacgagtcc agcgagatgg gtcttgagca taaatttgac ggcataattc
     3661 aagaaatacg ccccattctt cggaatcggt ttcaggatta aaagcaggaa cttcttttgc
     3721 tttttcaaac tcagcaccac ggacgacaag catagtcgcg tcgccaccat cgtctaaaat
     3781 aagattcgct gtgccgccgt ctggccaagc aagagctttt gcagtgttgt cccaatattc
     3841 ttctaaagat tcgcctttcc aagcaaaaac aggtgtccct ttaggggcat ctacagaacc
     3901 atttttacca acaacgacag cagcggccgc atggtcttgt gtactaaaga tattgcagga
     3961 aacccagcga acatcagcac caagctctgt tagagtttca atcaaaacag ccgtttgaat
     4021 agtcatatgg agagatccca taattctcgc gccagttaaa ggtttttgag ccccgtattc
     4081 acgacgaaga gccatcaagc ctggcatttc tgtttcagcg agttcaattt cttttcggcc
     4141 ccaagaggcg agattaatat ctttaacttt gtaatccata taaccttcta taaaaagacc
     4201 tcaaaagact ttcttagaca gttacagtct agatatctaa cgaggagatt gtaatatgca
     4261 taaatataaa aacttgcata tagaatccaa tggcactttt tttattgacc ataaaatagg
     4321 agatttttat taataaaaga tgatttttaa aaatggatac ggactttgat tagttttttt
     4381 ataactctac taaaaaaatt aatgtataac taaacctatc aaaaagaaat atcctaacac
     4441 ttaatttgat ccatgttttt ctaataacat tcagattcaa gaggggataa tgtttcaatt
     4501 actggagata tcaatgagtt ttaaaaaaat aggacttgtt cttttaggcg ttttttttct
     4561 tgctgcttgt tctggtgaaa atggagtgga tgggcgtgat ggagaaagcg tgaatgtcga
     4621 tagtttagcg gctgttttga gaggggaact atctcaggac ttgtgggatt ctatgcattc
     4681 agaaacggtt ttagattcag tttatgacac gctatttaat gatgctttta ctgatgtttg
     4741 gctagattca gctagaaatg ctttattaga tagcctttta gatgtctcgt acgattcgtt
     4801 gtatagtgtt ctttacgact cagtttacat tgatctttat gattctgtct ataaggatat
     4861 ttatgaaaaa agtgttacaa aagatttgtt ttcctatatc aattttaaaa aagaaaactt
     4921 taatacagct tttgctaatc aatactcttt aatgtataat ggttttgaac accctgttcc
     4981 attaggtgtt aaaattgaaa atagtgggaa tctatggcat accgttattg ttaaagcttg
     5041 gattcctgat tatagtgata caggaatagt ttctggcatt gtaaatccaa ataagtcaaa
     5101 cgtttttgct cctgagataa aattaatacc agaaaaatat ctccatttaa aagcggccgc
     5161 tccagtactt gtccaagtga gagcctttgc tttagaaaat aaccgagaaa ttcttatttt
     5221 ttcggaatcg tataatgcgg aaattcttcc agtagaactt tttggatcag aatatgtagg
     5281 tgttgatatt tctccttggt ggaatgtttg ggtgactcct aatatggatt ccatcacgtc
     5341 aatacattca gagcttgctt ctttaatgcc atcaggttat ggcgggtatc agctctatgg
     5401 ctttagtaca atggaagaaa gtgtgaataa tcaagtgaag aaagtgtatg aggttttaca
     5461 gaagaag
//
LOCUS       DAOOBB010000138         5393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_17448_length_5393_cov_4.217497, whole genome shotgun sequence.
ACCESSION   DAOOBB010000138 DAOOBB010000000
VERSION     DAOOBB010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5393
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17448_length_5393_cov_4.217497"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..1026)
                     /locus_tag="PLT31_06845"
     CDS             complement(7..1026)
                     /locus_tag="PLT31_06845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HPW94889.1"
                     /translation="MPQKMILCLVVFGLFLNACDSKSKRDEEKNRHVFSMTKFVSWYG
                     VQKNKTEQIPDASASLDTNSSIDTSLSNEVSLVDSSIVKSSSSTAKIEELQAKIDSFY
                     IIVENTSGSGMYFIGDTVSLFPQDKLDSGLCFDEWEVSDSKYLITNDLSDTAKVVAVK
                     DSLKVKAVYKSCYEGLESVVIGNLKRTTRNMNIWTFSGSWCYENKRKNCKRYGRLYDF
                     ETAQKVCRSGWRLPTDAEWNSLTQAAGNDPGTRLKSREGWIAEDGSTNSNGIDAIGFA
                     GLPAGIFYEGNFLYEGVYAYYWTATESNASMAWFRSLSYDNNEVYRHSNYKQAAFSVR
                     CVQDI"
     gene            complement(1053..2255)
                     /locus_tag="PLT31_06850"
     CDS             complement(1053..2255)
                     /locus_tag="PLT31_06850"
                     /inference="COORDINATES: protein motif:HMM:NF002325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinylornithine family
                     transaminase"
                     /protein_id="HPW94890.1"
                     /translation="MEQFCNKDCFDQDKDVIAPLYGKASINFVKGEGSYLFDADGHKY
                     LDFVSGIAVNALGHAFPAIVNAIQEQAAKFTHVSNLYPNELQVKLARKLLADSPYTKA
                     FFCNSGTEANEAAIKFARKYFNRIGQPDRVQIVTFFNSFHGRTFGALSATGQPALREG
                     FGSMPTDFSHVAWNDTSALKAMVNEKTCAIMLEPLAAEGGVLTISPEMVTTINDLRHE
                     FGCLVITDEIQTGLGRLGLFYGADTYGLKSDITTLAKAIGAGLPLGATLMRSKVADTL
                     KAGDHGTTFGGNPIACAAGLVVVNEIKKPLFLETVSIRSQQLKAGLSQLASKYSFLGD
                     VRGEGLLLGLCSEKPVADIITLLRKEGLLVHRAGANVLRFLPPLNVSAEEIEEALAKL
                     DRTFAKVV"
     gene            complement(2236..3012)
                     /gene="argB"
                     /locus_tag="PLT31_06855"
     CDS             complement(2236..3012)
                     /gene="argB"
                     /locus_tag="PLT31_06855"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HPW94891.1"
                     /translation="MKKVVIKIGGSLAVDEEKLSDFVTAVSSFSNKDYRLCVVHGGGK
                     DINENLALLSEKPTFIDGLRVTTPPIMNMVEMTLSGHVNKKLVRLFLSNGCPAIGISG
                     VDGSLIEVKKRNGEPDLGLVGEIVKVNPKIIHDLWNAGWTSIVSPISIGAGVSWNVNA
                     DTAASELAVALQADQFILVSDVAGVLDDQKKVISNLNETAVESLIAEGVISGGMIPKV
                     RESIKSLRRGLKSIHIVGWKDADHLKKQINGDFNYGTILQ"
     gene            3125..3322
                     /locus_tag="PLT31_06860"
     CDS             3125..3322
                     /locus_tag="PLT31_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HPW94892.1"
                     /translation="MAITKVWLDETNDECVSCGACEAICPDVFTVPDKMVVNESADFS
                     AYESEIQEAVDSCPSGVIKFS"
     gene            complement(3413..4981)
                     /locus_tag="PLT31_06865"
     CDS             complement(3413..4981)
                     /locus_tag="PLT31_06865"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94893.1"
                     /translation="MKSLLNVCLCVFISFFCWSCTEPTAHGVTDIGNSMAGVVVAEDG
                     KPLSNVRVVLYYDDWEAESISDSLETKTNTKGEFEFKNVNSEESFVILAKKESLSALS
                     LGSDTLVLRSPKFISSRILGKSGGWVRVLGQNQKQEVAEDGSFTFANTPSGELALIYG
                     ESEQGDLRFVFKTMDSRDSLLLPDLMEVESKEHWLQLADFRYYTEDGYDGIQAIDFYE
                     NEIEEFTLTYSLKEFVPQETLHHFVLPVRLDSTNFDFDSWDDSTEIMLTSTDEASLAF
                     EFDHFDPASKTALLWVRFDSIPSKAESVQIRFKKSAWLKQTPFQKEEGVMAVLHMEEL
                     ENLVSQDLPILSDSGFIGRGVSLAPSQYLDLDTLDPCSSDFTLSLWVYWYGKNGNHQI
                     LFSQRSFWSDSTSRFQWHFDYFNDVFAVYNNIDQRNKFTEVEVPVDEWAYLTLLFKEG
                     ELSMFVNGVRIGEPAAFVPTELNEAVPLRVGGNEVDVETWNGVLDEVRVEKVARSEEW
                     IRFSYEVQKAVKNK"
     gene            complement(4978..>5393)
                     /locus_tag="PLT31_06870"
     CDS             complement(4978..>5393)
                     /locus_tag="PLT31_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4423 domain-containing protein"
                     /protein_id="HPW94894.1"
                     /translation="IKLARSLDPVISTEEARVAVKNLEEWGLIRELSNGRYEATSLML
                     DATKVSPISLRQIRREYIQHAIGCIESKKKEERFSAFTTLSMTEESYAYAVNVLEEAR
                     KKIITRAAGDKEIEKVYELMVVLYPMSVLINKGDL"
BASE COUNT         1674 a         1198 c          940 g         1581 t
ORIGIN      
        1 atatttctaa atatcttgaa cgcagcgcac agaaaatgcc gcttgtttat aattcgaatg
       61 gcgataaact tcgttgttgt catagcttaa gcttctaaac caagccatcg acgcatttga
      121 ttctgtggca gtccagtaat aagcataaac gccttcgtac aagaagttcc cttcgtaaaa
      181 aatccctgca gggaggcctg caaaaccaat agcgtctatt ccatttgaat tagtggaacc
      241 atcttcggct atccatcctt ctcttgattt taatcttgtt ccaggatcat ttcctgcggc
      301 ctgtgttaag gaattccatt ctgcatccgt aggtaaacgc catccagaac ggcaaacttt
      361 ctgagcggtt tcaaaatcat ataatcgccc ataacgcttg caatttttcc gtttattctc
      421 ataacaccaa gagccagaga aagtccagat attcatattc cgtgtagtcc tcttcaaatt
      481 tccaatcact acgctttcta ggccttcata acaagattta tagactgctt tgacttttaa
      541 tgaatctttt acagcaacta cttttgccgt atctgaaaga tcatttgtga tgaggtattt
      601 tgaatcagag acttcccatt catcaaaaca taatccagaa tctagcttat cttgtgggaa
      661 aagagaaacg gtatcaccga tgaaatacat ccccgatcca gaggtgtttt ctacgatgat
      721 atagaacgaa tcaattttcg cctgaagttc ttctattttt gcagtagaag aggatgactt
      781 tacaatggaa gaatcgacta gagatacttc gtttgataga gaagtatcaa tagaagagtt
      841 tgtatctaaa gaggctgatg catcaggaat ttgttctgtc ttattttttt gaacgccata
      901 ccatgacaca aacttggtca tagaaaagac atgacgattt ttttcttcat cacgcttaga
      961 ctttgaatca caagcattca aaaacagccc gaatacgact aaacacagaa tcattttctg
     1021 aggcataaat cctccttaga gaaaaataga ccttaaacta cttttgcaaa tgtgcgatca
     1081 agtttagcaa gagcttcttc aatttcttca gcacttacat tcaaaggcgg caaaaaccga
     1141 aggacattag ccccagccct atgaaccaac aagccttctt tgcgaagaag agtaatgata
     1201 tcggcaacag gcttttctga acaaagacca agtaaaagac cttcgccccg tacatcacct
     1261 aagaagctgt atttagaagc tagttgtgac aagcccgctt ttagttgttg agagcgtata
     1321 cttaccgttt ctaagaataa aggtttttta atctcattga cgaccactaa gccagcggca
     1381 caagcaattg ggttaccacc aaaagtagtc ccatgatcac ctgcttttaa tgtatcggcg
     1441 actttacttc tcatcaaagt agcacctaat ggaaggccag ctccaatagc ttttgcaagc
     1501 gtggtaatat ccgattttaa gccataagta tcagccccat aaaagagacc taaccgacct
     1561 aatcctgttt ggatttcatc tgtaataact aaacaaccaa attcgtggcg taaatcatta
     1621 atggtagtta ccatttctgg actaatagtt aatactccac cttcagcagc gagaggctct
     1681 aacataatag cacatgtttt ttcattgacc atcgctttta aggcactggt gtcattccaa
     1741 gccacatgac taaaatcggt aggcatagaa ccaaagcctt cccgcaaagc aggctgacct
     1801 gtagcagaaa gagcaccaaa agtcctgcca tgaaaactat taaagaacgt tactatttga
     1861 acccgatcag gttgcccaat tctattgaaa tacttccgag caaatttaat tgccgcttca
     1921 ttggcttctg tacccgaatt acaaaagaat gcttttgtat aaggggaatc tgctaataac
     1981 ttgcgagcta atttaacctg aagttcattt ggatataaat tactcacatg agtgaattta
     2041 gctgcttgtt cttgaattgc atttacgatc gcagggaacg cgtgtccaag cgcatttaca
     2101 gcaataccag aaacaaaatc caaatactta tgcccatcgg cgtcaaaaag gtatgaacct
     2161 tcgcctttta caaaattgat agacgcttta ccataaagcg gggcgatgac atctttgtct
     2221 tgatcaaaac aatctttatt gcagaattgt tccataatta aaatctccat tgatttgttt
     2281 tttaaggtga tcggcatctt tccagccaac aatatgtatt gattttaatc ctcgtcgtag
     2341 agatttaata ctctctctca ctttaggaat cataccacca gaaataacac cttctgcgat
     2401 taaagactcg actgccgttt cgtttagatt tgaaatcact ttcttttgat catctaaaac
     2461 gccagccaca tcactcacta aaataaattg atctgcttga agcgcgacag ctaactcaga
     2521 cgctgctgta tctgcattga cattccaaga aacacccgca cctatagaaa taggactaac
     2581 aattgaagtc caacccgcat tccacaaatc atggataatt tttggattta ccttcactat
     2641 ttcgcctact aaaccaagat caggttcgcc gttccttttt tttacttcaa tcaaagaacc
     2701 atccacgcca gaaataccaa tagcagggca accattactt aagaacaaac gcactaattt
     2761 cttattcaca tggccagaca gagtcatttc aaccatattc ataattggag gagttgtaac
     2821 ccgaagacca tcaataaaag tgggcttttc agaaagcaaa gcgagatttt cattaatgtc
     2881 tttaccgccc ccatgcacca cgcacaagcg gtaatcctta ttagaaaaag aagacacagc
     2941 agttacaaaa tcagataatt tctcctcatc gacagcgaga cttccaccta ttttaataac
     3001 cacttttttc attcaaatgt ttcctttttg taaatattct ttaataagtt aggaaattaa
     3061 ttcccttctt gctacaattt attatattct tcgatgttaa taaaccatca acaaaggtat
     3121 aaccatggca attacaaaag tttggctaga tgaaactaat gatgaatgtg tatcttgcgg
     3181 tgcttgcgaa gcgatctgtc cagatgtttt tactgttcca gataaaatgg tcgtaaacga
     3241 aagtgctgat ttctcagcct acgaatcaga aattcaagaa gctgttgatt cttgcccttc
     3301 tggcgttatc aagttctcct aattcttttt ctccccagaa aaagttgcgc cccatcgttt
     3361 cttttattga aacaacaggg gtgcttcttt tttttaaaga ctaatgcaac ccttatttat
     3421 ttttaactgc tttttgaact tcataactaa agcgaatcca ctcttcagaa cgagctactt
     3481 tttcgacacg tacttcatct aatacgccgt tccatgtttc tacatcgact tcgtttcccc
     3541 cgacacgtaa agggacggcc tcatttaatt ctgtcgggac aaaagcggct ggctccccta
     3601 tacgtacccc attcacaaac atagaaagct cgccttcctt aaacaaaaga gtcaaataag
     3661 cccattcatc cacagggact tcgacttctg taaatttatt acgctggtct atattattgt
     3721 ataccgcaaa gacatcattg aaataatcaa aatgccattg gaatcgagag gtggagtcag
     3781 accaaaaaga gcgttgtgaa aataatattt gatgattgcc attttttcca taccaataaa
     3841 cccagagaga caaagtaaaa tcggaactgc aagggtctaa agtatctaaa tcgagatact
     3901 gagatggagc tagggaaacg ccgcggccaa taaaaccgct atcgcttaaa atgggcaaat
     3961 cctgactgac taggttttcg agttcttcca tgtgaagaac agccatcact ccttcttctt
     4021 tctggaatgg cgtttgtttt agccaagcgg attttttaaa tcggatttgt accgactctg
     4081 cttttgaagg aatggaatcg aaacgcaccc aaagaagcgc ggttttagaa gcggggtcaa
     4141 aatgatcaaa ctcaaaagca agagaagctt cgtctgtaga agtgagcatt atttcagtag
     4201 aatcatccca agaatcaaag tcaaaattcg tactatctaa acgcactggt aacacaaaat
     4261 gatggagcgt ttcttgtggt acaaattctt ttaatgaata ggtcaaagtg aattcttcga
     4321 tttcattttc ataaaaatca atcgcttgaa ttccatcata accatcttct gtatagtagc
     4381 ggaaatccgc aagctgaagc caatgttctt ttgattccac ttccattaag tctggcaaga
     4441 gaagcgaatc gcgagagtcc attgtcttaa atacaaaacg aagatcgccc tgttctgact
     4501 cgccataaat taaagccaat tcaccagaag gcgtgtttgc aaaggtaaac gaaccatctt
     4561 ctgccacttc ttgtttttgg ttttgaccga gcacgcgcac ccaaccacca gacttaccta
     4621 aaatgcgact cgaaataaat tttggtgaac gaagcacaag cgtatcggag ccaagagaga
     4681 gtgccgataa agattctttt ttcgctaaaa tcacaaagga ttcttctgag ttcacgttct
     4741 taaattcaaa ctcgcccttc gtgttcgttt tagtttctaa agaatctgaa atcgattctg
     4801 cttcccaatc atcataataa agtaccacac gaacgtttga taaaggtttt ccgtcttcgg
     4861 caaccaccac accagccatt gaattaccaa tgtctgtaac tccgtgagcc gtcggttctg
     4921 tacagctcca acaaaaaaaa gaaatgaaga cacaaagaca aacattcaaa agggatttca
     4981 tagatccccc ttattgatta acacgctcat cggatacaaa accaccatca gttcatacac
     5041 cttttctatt tctttatcac ctgccgcacg cgtaatgatt tttttgcgtg cttcttctaa
     5101 cacgttcacc gcataagcat acgattcttc tgtcatagaa agtgtagtaa atgcagagaa
     5161 cctttcttcc ttttttttgg attctatgca cccaattgcg tgctgaatgt actctcgacg
     5221 aatttggcgt aaagaaatag gggaaacttt tgtggcatcg agcattaaag aggtggcctc
     5281 atagcggccg ttgcttaact ctcgaattaa gccccattct tctaaatttt tgactgccac
     5341 gcgggcttct tctgtgctga ttacagggtc taaagaacga gcgagcttaa tat
//
LOCUS       DAOOBB010000139         5388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_17465_length_5388_cov_4.774423, whole genome shotgun sequence.
ACCESSION   DAOOBB010000139 DAOOBB010000000
VERSION     DAOOBB010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5388
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17465_length_5388_cov_4.774423"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..831)
                     /locus_tag="PLT31_06875"
     CDS             complement(190..831)
                     /locus_tag="PLT31_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94895.1"
                     /translation="MKKFLLLILCFTVFSFADNFWTRTHYFGLSFSGFLTTGDLSKNK
                     TISLDEETVTEVFHFPDFKHSFIPEAEVGVNLNAHTFAFVVGLYPFESDYSYLKLGAD
                     YRYYLFWPNPLQIGFGINYTYMRIDSKNNVVTSSPENEKPVVSDGVLMGNGVSALSSV
                     RYYFTEHLGAEFSLKYRFFFYSSLNTEKCGTCSIDNSLNQHFGEAGIKLFFQL"
     gene            complement(952..1968)
                     /gene="nadA"
                     /locus_tag="PLT31_06880"
     CDS             complement(952..1968)
                     /gene="nadA"
                     /locus_tag="PLT31_06880"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HPW94896.1"
                     /translation="MTAQELYQRLKTIQPGSVLCNYSMEACENMIPMINEIIELKKQQ
                     NAVIFAHSYVSPEIIYSVADFDGDSFKLSQDATKINADRIIFAAVRFMGETAKILNPH
                     KQVLIPGPLTGCSLADSITVNQIEELHRIYPNHTFVCYINTTAEIKAQCDVCVTSSNV
                     IDIFSHIENDKIVFVPDHLMGQNVKAELEKKGIHKEIVLFDGCCYVHEQYDPEMIRFF
                     REQNPGLKVISHPECHPGVALLSDFVGSTGQMVQYIRKSPPHTPFLLLTECGLNARMQ
                     SEMPDHTFIGSCSMCKYMKSNSLSNILETLRHPEKALSIEIEESVQIKAKKCIDAMFH
                     YASL"
     gene            complement(2208..2858)
                     /locus_tag="PLT31_06885"
     CDS             complement(2208..2858)
                     /locus_tag="PLT31_06885"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPW94897.1"
                     /translation="MKRSLFIGDVHGCYKELVQIIEDFGFVKGSDTIYQTGDMINKGP
                     HVLKCIELTQELGIQCVLGNHEARLLKTLDTPKIKWTEKERERLKRITNIDYVYSVIS
                     KWPLWIDTPEALLVHAGIEPYKDKLEDMNPDVLLSVRLWDNQPWFKQVEWPKKIVFGH
                     WAKKGLVNLPGFIGLDSGCVYGKALTAWCPEEDRFYTVQAQKVYSEINEYAYSSLS"
     gene            complement(2855..3556)
                     /locus_tag="PLT31_06890"
     CDS             complement(2855..3556)
                     /locus_tag="PLT31_06890"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPW94898.1"
                     /translation="MRLNKFISSCGIASRRAADTLIAEGRVMVNNEVVRELGSQVSPS
                     DTVLVDGKLAHLPQKTTILLFHKPPGCVCSASDPQGRKTVYDYLPPGYKGLKYIGRLD
                     LQSRGLLLFTDNGELAHRLTHPSYQIQRSYLVWSEPPLSKADAEKIIDGVDIGDGETG
                     HAEAIYLKNGFVEMVLTEGKNREIRRMMESLGYQIKDLKRISFCGILLGSTPVCDFRE
                     LSAEEVQNILKVCEL"
     gene            complement(3543..4454)
                     /locus_tag="PLT31_06895"
     CDS             complement(3543..4454)
                     /locus_tag="PLT31_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HPW94899.1"
                     /translation="MPNKLLASIDIGSHSCILLIAAFEKQENGQEKLVPKLQKVEVCR
                     LGEDVYEFGAISEERLNDLIIILERFRSTVHALGVDLKAVAMTEAMRKASNSDEVILA
                     VEKALWFKPQIISGEEEAALTYKAISEWYHSELLTLDIGGGSTELCLRNKCLSIPVGA
                     LYLFKKMGAIPGPEYKNFVKETFKEYPVRAFSKKEVVFIGGTATALAMVYLNLNQFKY
                     EQIEGLEMTLNDLEQTTMRISNLSKELRALLPGLENGRSDVIICGLYWLKSLLERLHV
                     ESFKISTAGIRYGLLYPSPSEESHEIK"
     gene            complement(4444..4782)
                     /locus_tag="PLT31_06900"
     CDS             complement(4444..4782)
                     /locus_tag="PLT31_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94900.1"
                     /translation="MISEKDLQEFESLDLYEKISRIEQRLEGKENPKPFELGMLLALK
                     MAVEIREQKELGSESAVLVARWADLYPESVVEEAISNAKEFLLHSTSLVEKIRESLIG
                     DDPKEDSGAK"
     gene            complement(5117..>5388)
                     /locus_tag="PLT31_06905"
     CDS             complement(5117..>5388)
                     /locus_tag="PLT31_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94901.1"
                     /translation="AAPKKAAAPKKAAAPKKAAAPKKAAAPKKAAAPKKAAAPKKAAP
                     KKAAAPKKAAAPKKAAAPKKAAPKKAAPKKAAPKKAAAKKAAAKK"
BASE COUNT         1742 a         1012 c          968 g         1666 t
ORIGIN      
        1 attgattaaa cccacatcca tcgcaaacca tcataatcac attcttactt ttgcagcctt
       61 tcaccatttc tttataatct aaggctcctt ctccatcaat taatccgcaa gaagaaagta
      121 aaaaaagact tgagcttata acggcttttg aaaaaaagtg tttcattaat ctaaaatcca
      181 cagaaaaatt tataattgga aaaatagttt aattcctgct tccccaaagt gttggttaag
      241 cgaattgtct atagaacatg ttccacattt ttctgtattt aaactagaat aaaagaaaaa
      301 gcgatatttt aaggaaaatt cagcaccaag atgctctgta aaataatagc gcaccgaaga
      361 aagcgcagat actccattac ccattaaaac gccgtcactt acaacaggtt tttcgttttc
      421 tggagaagac gttacgacat tatttttaga atcaattctc atataggtat aatttatgcc
      481 aaaaccaatt tggagagggt tgggccagaa caaatagtaa cgatagtcgg ctccaagttt
      541 taaataagag tagtcggact caaaaggata aagccctacc acaaaagcaa aagtgtgcgc
      601 attcagattc acgcccactt ctgcttcggg aataaaagaa tgtttaaaat caggaaaatg
      661 aaatacttct gttacagtct cttcatctaa ggaaatcgtt ttgttttttg aaagatctcc
      721 tgtagttagg aagccactaa aacttaagcc aaaataatgc gttcttgtcc agaaattatc
      781 agcaaaagag aaaaccgtga agcataatat taagagaagg aattttttca taaaaggact
      841 tcctttttat gttaaagacg aaaatttaag tctacttcaa aatagttttt tcaaaaataa
      901 tgaaaggctt tttctgatca aattccctta ttaaataatc acttcgacaa actatagaga
      961 agcgtaatga aacatcgcat caatacattt ttttgcttta atttgaacag attcttcaat
     1021 ttcaatagag agagctttct caggatggcg aagcgtttct aaaatattgg atagtgagtt
     1081 agacttcata tacttacaca tactgcaact gccaataaat gtgtgatctg gcatttccga
     1141 ttgcatgcga gcatttaagc cacactcggt gagtagtaaa aatggggtat gcggaggtga
     1201 ttttctgatg tactgcacca tttgacctgt gcttccgaca aaatcactaa gtaaagcaac
     1261 gcctgggtgg cattctggat gactaattac ttttagccca ggattttgct ctctaaagaa
     1321 acggatcatt tctggatcgt actgttcatg aacatagcaa cacccgtcaa acaaaacgat
     1381 ttctttatga atgcctttct tttcaagttc cgctttaacg ttttgcccca ttaaatgatc
     1441 gggaacaaag acaattttat cattttcaat atgagaaaaa atatcgataa cattagaact
     1501 agtcacgcag acatcgcact gcgccttaat ttctgcagta gtattaatat agcagacaaa
     1561 agtatggttt ggataaatac gatgaagttc ttcaatttga tttacagtaa tactatctgc
     1621 taagctacag ccagtgagag ggccaggaat taacacttgt ttatgagggt ttagaatttt
     1681 tgctgtttct cccataaaac gaacagcagc aaaaataata cgatcggcgt taatttttgt
     1741 ggcgtcttga cttagtttaa aactatcgcc atcaaaatcg gcgacagaat aaatgatttc
     1801 tggagaaacg tagctatgag caaaaataac tgcattttgc tgttttttta actctataat
     1861 ttcattgatc ataggaatca tgttttcaca agcttccatg ctgtaattac ataaaacgct
     1921 tccaggctgg atcgttttaa gacgttggta taattcttga gctgtcatag tgtaaaatgt
     1981 agaaaattga ggactgataa ttatttttta ataatcgttt tttacttgtt tcgggaaaat
     2041 catttcgaga gactcgtttt gaggaaagta ttgtcgaata atgctaagac taattaaggc
     2101 aagttttgaa aatgacgcct gtttcaggag aatattccca cgagactctt tttaaatgag
     2161 ttgtttttca aattaaattt tttaagaatc aaagaaagtc agaataatta agataaagac
     2221 gaataagcat attcattgat ttcagaataa actttttggg cttgaacggt ataaaaacga
     2281 tcttcttcag ggcaccacgc tgttaacgct ttaccgtaaa cacaaccaga atcaaggcca
     2341 ataaaaccag ggagattcac aagccctttt ttggcccaat gcccaaaaac gattttttta
     2401 ggccactcta cctgtttgaa ccatggttga ttatcccata aacgaacaga aagcaaaaca
     2461 tcgggattca tatcttccag tttatcttta tagggttcta tccctgcatg aactaaaaga
     2521 gcttctggag tatcaatcca tagaggccat ttagagatca ccgaataaac ataatcgata
     2581 ttagtaatcc ttttcaagcg ttcccgttct ttttcagtcc attttatttt aggagtatct
     2641 aaagttttaa gaagacgagc ttcatgattg cctagtacac attgaattcc aagttcttga
     2701 gttaattcaa tgcattttaa aacatgaggc cctttattaa tcatatcacc tgtttggtat
     2761 atggtatcgg agcctttcac aaaaccaaaa tcttcgatga tttgaacgag ttctttataa
     2821 caaccgtgaa catcgcctat aaaaagagaa cgtttcataa ctcacaaact tttagaatat
     2881 tttgcacttc ttcagcagat aattctctaa aatcacaaac gggagtcgaa cctaaaagaa
     2941 ttccacaaaa agaaattcgt tttaaatctt taatttggta accgagactt tccatcatgc
     3001 gacgaatttc tctgttttta ccttccgtaa gtaccatttc tacaaaacca tttttaagat
     3061 aaatcgcttc tgcgtggcca gtttctccat cgccaatatc caccccatca ataatttttt
     3121 cagcatctgc tttagaaaga ggaggttcac tccacactaa atagctacgc tgaatttgat
     3181 aacttgggtg tgttaaacgg tgtgcgagct ctccattatc agtaaaaagc aaaagaccac
     3241 ggctttgcaa gtcgagtcgt ccaatatatt ttagcccttt ataacctgga ggtaaataat
     3301 catataccgt ttttctccct tgaggatcag aagcggaaca aacacatcca ggaggtttat
     3361 gaaataaaag aatggtggtc ttttgaggta aatgggcgag ttttccatca actaaaaccg
     3421 tatcggatgg agaaacttga gaacctaatt ctcgaacaac ttcattattc accatcacac
     3481 gcccttctgc aataagggta tcagcggcac gcctagaggc aataccacaa gaagatataa
     3541 acttatttaa tctcatgaga ttcctcagaa ggagaagggt atagcaaacc atatcgaatg
     3601 ccagcggtgc taattttaaa agattcgacg tgaagccttt ctaataaact ttttagccaa
     3661 tataaaccac aaataattac atcactacgg ccattttcaa gacctggtaa aagagcgcgc
     3721 aattctttgg aaagattaga aattctcatc gtagtttgct ctaaatcgtt taaagtcatt
     3781 tcgaggcctt cgatttgttc atatttaaac tgatttaagt ttaaatacac catcgcaagt
     3841 gctgtagctg ttccaccaat gaaaacgact tctttttttg aaaaagcacg cacagggtat
     3901 tctttaaacg tctctttcac aaaattctta tactctggtc caggaattgc ccccatcttt
     3961 ttaaataaat agagagcacc cacgggaata gataaacatt tatttcttaa gcaaagttct
     4021 gtagatccac caccaatatc taatgtcagt aactcagaat gataccattc gctaatggct
     4081 ttatatgtaa gagcggcttc ttcttcacca gaaatgattt gaggcttaaa ccacagagct
     4141 ttttctacag ctaaaataac ttcatcacta tttgatgctt ttcgcattgc ttctgtcatc
     4201 gccactgcct taagatctac tcctaaagca tgaacggtcg agcgaaaacg ttccaatatt
     4261 ataattaaat cgtttaatct ttcttcagaa atggctccaa attcataaac atcttctccg
     4321 agacgacata cttcgacttt ttgaagttta ggaacgagct tttcttgacc gttttcttgc
     4381 ttttcaaaag cggcgattaa taaaatacag ctatgactac cgatatctat agaggcaagt
     4441 agtttatttg gcacctgaat cctcttttgg atcgtctccg atcaaagatt ctcgaatttt
     4501 ttcaacaaga gaagtagagt ggagtagaaa ttcttttgca ttagaaatcg cttcttcaac
     4561 aacggattcg ggataaaggt cagcccatct tgcgactaaa accgcagatt cagagccgag
     4621 ttctttctgt tcgcgaattt ctacagccat ttttaaagca agtagcattc ctaattcaaa
     4681 tggtttggga ttttctttcc cttcgagtcg ctgttcgata cgggaaattt tctcgtataa
     4741 atctaaactt tcaaattctt ggaggtcttt ttctgaaatc atgctaataa atttataaaa
     4801 atatctatag ggtacttcaa aaaatcgctt tggtgaagaa aaattgattg cgaacaagcg
     4861 gaagaaggaa cgagaagcgg aaaatactgg tggtatttgc gtcggcgcga cccacatttt
     4921 aaagtgtttt gatgaaatgt gtgtatgcta taaagcagat tatagagtgg aaaaaaagga
     4981 taaacagaaa agtggagaga atacttgcga gaacttgcat ttgcatgact agcggagtat
     5041 ataggaatgt ttaggatgtt taaaaaataa aagagagccg taaagactct cttttatttt
     5101 taatgctttg aaagaattat ttcttagcag cagctttctt ggcagcagct ttcttaggag
     5161 cggctttctt aggagcggct ttcttaggag cagctttctt aggagcggca gctttcttag
     5221 gagcggcagc tttcttagga gcagcagctt tcttaggagc ggctttctta ggagcagcgg
     5281 ctttcttagg agcagcagct ttcttaggag cagcggcttt cttaggagca gcagctttct
     5341 taggagcagc agctttctta ggagcagcag ctttcttagg agcagcag
//
LOCUS       DAOOBB010000140         5385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_17478_length_5385_cov_4.538274, whole genome shotgun sequence.
ACCESSION   DAOOBB010000140 DAOOBB010000000
VERSION     DAOOBB010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17478_length_5385_cov_4.538274"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..1409)
                     /locus_tag="PLT31_06910"
     CDS             complement(135..1409)
                     /locus_tag="PLT31_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPW94902.1"
                     /translation="MPLSYTIILIVFFLLLSAAYSLVKVVFLAIHSRKEFGELTKREE
                     RINAYVESSGFNECMSIGRIFSNIIIAVLGYIAAEPLISSFFSPTLALALYLLVTGLS
                     VYFITVFIPNLFGHLKPGMLSIILEPIYKWIRFPFFGFSRLVHFVYNKLLKWFGYDAK
                     FSFLSENQREAIQSDMSSSKEEGSLEDDERQMILNIFDFVETPVREIMTPRVDMCAID
                     VKSSLETTIQILNNERHSRLPVYKDSIDNIIGILSNRDFLEWYTEHGSEPFNIEPLVM
                     PAFFVPYYKKIDDLLTELRRNGNQLAIVIDEYGGTAGLVTVEDILEEIVGEIKDEDDE
                     DEGVQKLKDGRYILDPLMTLSDLEYELKIKLKVPENSHIETLSGLIQATLGTIPSPGV
                     EVEIEGYKIRVLSMDGTRMEKVLLITPTPSTN"
     gene            complement(1415..1861)
                     /gene="ybeY"
                     /locus_tag="PLT31_06915"
     CDS             complement(1415..1861)
                     /gene="ybeY"
                     /locus_tag="PLT31_06915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HPW94903.1"
                     /translation="MPDPSSSFNVTFLCEGEIARFPWQSRLEPMARKLLIEEGFPQNV
                     NVVLCSDEFVREMNKQYRNLDKVTDVLSYEWHEPYLLGEIYIAENQVKKQAPLFGNTY
                     YAELKRVLVHGLFHLCGYDHLTPKDRRKMRLRECGFLGIDPYSERE"
     gene            complement(1862..2149)
                     /locus_tag="PLT31_06920"
     CDS             complement(1862..2149)
                     /locus_tag="PLT31_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94904.1"
                     /translation="MNMDNTEPTVEMTDENGELIVKELAKEYLTKGAWQTIMFLYQEK
                     DQKTGEFAEPKVSIRRYQKYQGVMKQRSKFNVSNKAQAEAMIAIFKKWYEI"
     gene            2274..4148
                     /locus_tag="PLT31_06925"
     CDS             2274..4148
                     /locus_tag="PLT31_06925"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HPW94905.1"
                     /translation="MKRALFIIISVLLITVPLVADEITSGKVRSVLGEVTRQKKAQDK
                     WLPLRVGAKVSESDLIRTLVESQAIISLADGSSITIEENAIVVLEKVFSAKGSKETSL
                     NIKNGMLHFDVQKQAGKSGSFKFKTGTATAAIRGTAGAIGVTRKGLYASLAEGSLEVE
                     DAHGNKALLKKGQTLVQDSSKGFIVLSNPLSGTPDFSRTLHEILSDSSSSQEMSPELL
                     DSLSQKLNSQIKSLLDKSKCQFNTISDTVYSTELKLQGRCNPGIMLSIDGEQTAPTLD
                     SLIEHTISWDTGLFGEKKYRIYCVDNQVEAQCGEIQFIYADSAGKKVLKKEAKNNFII
                     QSPTSIHLCESNTIQIEGTFEKASDDTELRIKIGNAISENLIPLNENQKFFHSILVND
                     LSKNWNEKTIDIEMTNAQGLIKKETIALDINKSCKTINVISPKIKWISSDSLMCKAFI
                     KITDAEGDEILLSKYTNSNLSSESYHIGNQNKIELSLTPGIHEYRFKVQDLAKNEQEI
                     TKTLGCFPKHQVQIQVYGGSSEVWRVPPPPKEASNILNKELRFSIKKVPENDTRYIQQ
                     IIVKKNGREIFKRVKDQITDLHYNLPIEISRGSRNVFDIEVRLKSGNVSKAIKTYEVR
                     "
     gene            4148..4789
                     /locus_tag="PLT31_06930"
     CDS             4148..4789
                     /locus_tag="PLT31_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94906.1"
                     /translation="MINKYITGFTLLLLSTILLAQQPDTLIRPEVSNSDSILNTINPV
                     ADSSNAPNISNSIANLNIINQATDSTIKPKISESTPDLLTKQEKKPVPSYTTLSGEVH
                     GFLTKDKSPYLVTQSISVPLGRVLIIEPGVVILFKEGASFSIEQGQLIAGGTRSTPIY
                     FKGTDSSTVWNGLSIKGEKKSDLRYVKISGAKTAIMLKEGSLNIHNCIIQNSA"
     gene            4853..>5385
                     /locus_tag="PLT31_06935"
     CDS             4853..>5385
                     /locus_tag="PLT31_06935"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94907.1"
                     /translation="MGAHLATHNESYFEENRFLNNEIGLYLSPLSNTQIVSTTWKENQ
                     LALLYMINSKLVLHRCSIENNEVGISTSEMLRPESREQVKNNKINFLSDTKLFSSKIP
                     LPEGIDLSIKEEPKAKESSVTFTANDSTKWSIAGNISAGLNYRYVYTRHNHGVLNEIV
                     NEDTIHVKEDRFPNLWEV"
BASE COUNT         1907 a         1040 c          864 g         1574 t
ORIGIN      
        1 aatcaagaag ggaaagtcat tcgtcatgcc aatgaacttt cacctggaaa taaaattaaa
       61 actcgattct acgatagtac catagactct gttgtctctt aataaaagac tcgcaaaact
      121 atttttagaa cagcctaatt cgttgaaggc gtaggcgtaa tgagaagaac tttttccata
      181 cgcgtaccat ccatggaaag tacacgaatt ttatagcctt caatttccac ttctacacct
      241 ggagaaggga ttgttccaag agtcgcttga attaagccag ataatgtttc aatatgagaa
      301 ttctcaggca ctttcagctt aattttcaat tcgtattcta aatcagaaag tgtcattaaa
      361 gggtctaaaa tataacgacc atcttttaac ttttgaacac cttcatcttc atcatcttca
      421 tctttaattt cgcccacaat ttcttcaaga atatcttcaa ccgttacaag ccctgccgta
      481 ccaccatatt catcaatcac aatggcgagt tgatttccat tcctgcgaag ttcagttaat
      541 aaatcatcaa tcttcttata gtaaggaaca aaaaatgcag gcatcactaa cggttcaata
      601 ttgaatggtt cagagccatg ttcagtatac cattctaaaa aatctcgatt agataaaata
      661 cctataatat tatcaataga atctttataa acaggtaaac gagaatgacg ttcattattc
      721 aatatttgaa ttgtcgtctc tagagaagac ttgacatcaa tcgcacacat atctacacgt
      781 ggagtcatga tttcgcgaac aggtgtttct acaaaatcaa aaatattgag aatcatttgg
      841 cgttcgtcat cttccaaaga cccttcttct tttgagctac tcatatcgga ttgaatagcc
      901 tctctttgat tttcagaaag gaaactaaat ttagcatcgt aaccaaacca ttttagcagt
      961 ttattgtata cgaaatgaac taaacgggaa aaaccaaaaa aaggaaaacg aatccacttg
     1021 tagataggtt caagaatgat ggagagcatc ccaggtttta aatgtccaaa caaattcggg
     1081 ataaaaaccg ttataaaata aacactcaaa cccgtaacaa gtaagtaaag agctaacgct
     1141 aaagttgggg aaaagaaaga tgaaatcaac ggctctgcag caatataacc aagcaccgca
     1201 attatgatat tcgagaatat acggcctatg gacatgcatt cattaaagcc actcgattca
     1261 acataggcat taattctctc ttctcttttt gtgagttcac caaactcttt acgagaatga
     1321 atcgctaaaa aaacaacctt cactaaagaa taggctgcgg aaagtaaaag aaaaaaaacg
     1381 attaaaatga tagtataact taaaggcata aatattactc tctttcgcta taaggatcta
     1441 tacctaaaaa gccgcactct cgtaaacgca ttttacgacg atccttggga gttaaatggt
     1501 cataaccaca taaatggaat aagccatgca caaggactct ttttaattca gcataataag
     1561 tattgccaaa tagaggggct tgttttttta cttgattttc agcaatgtaa atctcaccta
     1621 gcaaataagg ctcatgccac tcataagata gaacatctgt gactttatcg agattccgat
     1681 actgtttatt catttctctg acaaattcat cagagcataa aaccacattc acattttgag
     1741 gaaagccttc ttcgataaga agctttcgtg ccataggttc aagacgagat tgccatggga
     1801 atctcgctat ttcaccctca cataaaaaag taacattaaa actactactg gggtcgggca
     1861 tttatatttc gtaccacttt ttaaatatgg cgatcatcgc ttccgcttgc gccttattgc
     1921 tcacgttaaa tttgctgcgt tgtttcataa cgccttgata tttttgatac cgacgaatag
     1981 atactttagg ttcagcaaat tcgcctgttt tttgatcttt ttcttgataa agaaacatga
     2041 tcgtctgcca agcaccttta gtcaaatact cttttgcaag ttccttgact atcagttcac
     2101 cattttcatc agtcatttca acagtcggtt cagtgttatc catattcata aaaaacctct
     2161 tattattatt aataaaatag aaagtttcaa agataaaata atgtaaagat gtacttacga
     2221 agccgaaata ctatattatt tataggaatt ttaaaaatcc cctcggagga ttaatgaaac
     2281 gagccctatt tattatcatt tctgttcttt taatcactgt ccctttagtg gcagatgaaa
     2341 ttacctccgg aaaagttcgt tctgtgttag gagaagtcac tagacaaaaa aaagctcaag
     2401 acaaatggct tccacttcgt gttggagcta aagtctctga gtccgattta attcgaacac
     2461 tcgtcgaatc ccaagccatc atttcccttg ctgatgggag ctctattaca atcgaagaaa
     2521 atgcaatagt cgttttagaa aaagtatttt cagctaaagg atctaaagaa acctcattga
     2581 atatcaaaaa tggaatgctc cactttgatg tacaaaagca agctggaaag tctggttcct
     2641 ttaaatttaa aactggaact gcaactgcag ccattcgagg tacagctgga gcaattggag
     2701 tgactcgaaa aggtctttat gcttcccttg ccgaaggctc tcttgaagta gaggatgcac
     2761 atggaaataa ggctctttta aagaaagggc aaacactcgt acaagattca agtaaaggat
     2821 ttattgtcct aagcaatccc ctctctggaa ctccagattt ttcaagaacg cttcacgaaa
     2881 ttctcagcga ttcatcgtcc agtcaagaaa tgagtcctga acttttagac tctctaagtc
     2941 aaaagctcaa tagtcaaatt aaatctcttt tagataaatc aaaatgccaa tttaatacga
     3001 tttcagatac ggtatactca acggaactaa aactccaagg aagatgtaat ccaggcatta
     3061 tgctttccat tgatggagaa caaacagctc caactctaga tagtcttata gaacacacca
     3121 tctcttggga tactggttta tttggagaaa aaaaataccg tatctactgt gtagataacc
     3181 aagtcgaggc tcaatgtggt gaaattcaat tcatttatgc cgatagtgca ggcaaaaaag
     3241 ttctaaaaaa agaagccaaa aataatttta taattcaaag tccaacttca attcacttat
     3301 gtgaatcaaa taccattcaa attgaaggaa cgtttgaaaa agcaagcgat gacactgaat
     3361 taagaattaa aatcggaaat gctatctctg aaaacctaat tcctttaaac gaaaatcaaa
     3421 aattcttcca ctctatttta gtgaatgact tatcgaaaaa ttggaatgag aaaacaattg
     3481 acatagagat gactaatgct caagggctta ttaaaaaaga aaccattgct ttggatatta
     3541 ataagtcatg taaaacaatt aacgttattt ctccaaaaat aaaatggatt tcttcagact
     3601 ccttaatgtg taaagccttt ataaaaatca cagatgctga aggggatgaa atattattat
     3661 ccaaatacac caattcaaac ttatcctcag agtcttatca tatagggaat caaaacaaaa
     3721 ttgaattatc tctaactcca ggaatccatg aatatcgatt taaggttcaa gacttagcaa
     3781 agaatgagca agaaatcaca aaaactcttg gatgcttccc aaaacaccaa gttcaaattc
     3841 aagtctatgg tggctcttca gaagtttggc gagttcctcc tccacctaaa gaagcaagca
     3901 atattctaaa caaagaatta cgcttctcaa taaaaaaagt acctgaaaat gatactcgat
     3961 atatccaaca gattatagtc aagaaaaatg gtagagagat ctttaagcgt gtgaaggatc
     4021 aaattaccga tttacattat aatctcccca tcgaaatatc tagagggtca agaaatgttt
     4081 ttgatattga ggttcgctta aaaagtggaa acgttagcaa agctattaaa acctatgagg
     4141 tacgataatg ataaacaaat acattaccgg attcaccctt ttattattaa gtaccattct
     4201 acttgctcaa caacctgata ctttgatcag gcctgaggta tcaaatagcg attctattct
     4261 caatacgatt aatccagtcg ctgattcttc aaacgcacct aacatttcaa atagtatcgc
     4321 taatctaaat attattaatc aagcaactga ttcaacaatc aaaccaaaaa tttcagaatc
     4381 gacacccgat ttattaacaa aacaggaaaa gaaacctgtt ccaagttata caacgctctc
     4441 tggcgaagtt catggattct taacaaaaga caagtctcct tatctagtaa ctcaatctat
     4501 ctcggttcct cttgggagag ttttaatcat agaacctggt gttgtaattc tttttaaaga
     4561 aggagcctct ttttctattg aacaagggca actcatcgct ggcggcacaa gaagcactcc
     4621 catttatttc aagggcactg attcctctac cgtgtggaat ggtctttcga tcaaaggaga
     4681 aaagaaatcc gatcttcgct atgtaaaaat aagtggagcc aaaacagcga tcatgttaaa
     4741 agaaggctct ctaaatattc ataattgtat aattcaaaat tcagcataac gaggactgtt
     4801 tgctattcaa tccaacttaa aaatcactaa aacccttttt agaaacaatg ccattggagc
     4861 tcatttagca acacataatg aatcctattt cgaagagaat cgttttctta ataatgaaat
     4921 tggcttatat ctatctcctc tatccaatac acaaattgtt tccactacat ggaaagagaa
     4981 tcaattggct ctattatata tgatcaatag caaattagtg ctccatcgtt gctctattga
     5041 aaataatgaa gtgggaataa gtaccagtga aatgctccga ccagaaagcc gcgagcaagt
     5101 aaaaaataat aaaatcaatt ttctttccga caccaagtta ttctcttcaa aaattcctct
     5161 gccagaaggc atagatcttt cgataaaaga agaacctaaa gcgaaagaat cctctgtaac
     5221 attcactgca aacgactcca ctaaatggtc aatcgctgga aatatctctg ctggactaaa
     5281 ctatcgatac gtatacacta gacacaatca cggtgtatta aatgagatag tgaatgaaga
     5341 tactattcat gttaaagaag atcgtttccc taatctttgg gaagt
//
LOCUS       DAOOBB010000141         5359 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_17584_length_5359_cov_4.329940, whole genome shotgun sequence.
ACCESSION   DAOOBB010000141 DAOOBB010000000
VERSION     DAOOBB010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 5359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5359
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17584_length_5359_cov_4.329940"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="PLT31_06940"
     CDS             complement(<1..891)
                     /locus_tag="PLT31_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPW94908.1"
                     /translation="MMRLYTEETTKELSRTEFLAMMADSTSVITQLTLQKTPDGVVIE
                     GKREMNAEEAKAYSSQSKPFSRFAQAEKDKTPSVFFKSHMLEISNEQITAWEMFKNVK
                     VKVIHETTSWVDHLIAFLPIIILIAFFWFMMSRQSGGGKGMFSFGKSQAKQLVQNGKN
                     KTTFRDVAGCDEAKQDLEELVLFLKDPKKYDALGGRIPKGALLVGPPGTGKTLLARAV
                     AGEAGVPFFSMSGSDFVEMFVGVGASRVRDLFETGKKNAPCILFIDEIDAVGRQRGAG
                     LGGGHDEREQTLNQLLVEMDG"
     gene            complement(969..2210)
                     /gene="tilS"
                     /locus_tag="PLT31_06945"
     CDS             complement(969..2210)
                     /gene="tilS"
                     /locus_tag="PLT31_06945"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HPW94909.1"
                     /translation="MLLQKDVELFYGKSLLLGVSGGVDSICLLHYFYSHYKELNVSKI
                     YVCHVNHGVRGKNADDDASLVKSLCESYQIPFEIQNIDPSLFNSKESFESKARELRYQ
                     ILFDIQKKQKLDYILTAHHADDQAETLYMRIRRGSSLKGLQGILAKRDDLVVRPFLHL
                     TKSSLIQYAKNHQLKWREDDSNQKTIFARNKTRLIILPYLESKTPGIMSILAHTASLS
                     HEVYPKVLEKANHFFKTIEVSKENWPFPANYSPYSKVLCISSKKWNEAYAKMGNGLIE
                     ILRLKLDSMGIYFPMDDFLHFIQTENQNRYHYRESHIEKSKFFLWFYSTADAQNIDNL
                     YLFEKNKRFSGEWRYRQEGDRFTPPGSKYKKRKLKQWLQEKGIPLWIRDYLPLLVQNA
                     DVLWIPGVAVSGKLEPTERTP"
     gene            complement(2189..2521)
                     /gene="hisI"
                     /locus_tag="PLT31_06950"
     CDS             complement(2189..2521)
                     /gene="hisI"
                     /locus_tag="PLT31_06950"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HPW94910.1"
                     /translation="MTFEETLSEIKFDAQGLVPAIVQDATKLDVLMMAWMDKEALKRT
                     VECGEMVFWSRSRKEYWHKGDTSGNVMTVVEWAVDCDFDVLLFKVKMQGPQVACHTGA
                     RSCFFRKM"
     gene            complement(2549..3808)
                     /locus_tag="PLT31_06955"
     CDS             complement(2549..3808)
                     /locus_tag="PLT31_06955"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HPW94911.1"
                     /translation="MTEIEQYGLELANNARNASQTVRVLPIETRNSVLLSVAEKLRNE
                     VPSILKANKNDVEHSKGVISDAMIDRLTLNPARIESMARGVEEIAAFADPLNKVLEKN
                     TLTNGIQLSRVSVPIGSVFFIYESRPNVTIDGASLCFKSGNAVILRGGKESLETSTKL
                     ANIFRSALEENHVNPNAVQLVNTPDRSLMSFLLQQNQSIDLVIPRGGENLIKAVVEQS
                     RIPVIKHFNGICHIFVDKSANFSKTAAILFNAKTQRTGVCNAMETLLIHRDIDDKTVE
                     QFISPLREKGVEMFGDSEAQKRISGVKDIGDESNYHHEYLALKMSVKFVANVQEACEH
                     IEKYSSRHTDAIIAENKEVQDYFIANVDSSSVMLNASTRFADGGEYGLGAEVGISTDK
                     LHARGPMGVESLCTYKWVLRGNGQIRE"
     gene            complement(3827..5260)
                     /locus_tag="PLT31_06960"
     CDS             complement(3827..5260)
                     /locus_tag="PLT31_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94912.1"
                     /translation="MKNKILLSVFIFSLIPTLLFALPNRTEAKGHLAFSDSTGTKNLN
                     VRISLIDSLRGTNYKNSSVYYDNSAIFKLSVMGELSQSFRFESHNELISDYTNRFINS
                     NHFNPSDGIPYTKESDNSKGWNRFRASLEYDLPFTTILAGYDYLQWGVAKRNPVILRG
                     SNHIYRPWMDTTSFIFEPAPTPYFGYQFEFGRLSYTQYATKLYHKKNTGKYLNAHRLQ
                     INLPFDIELGASEMIIYGTTTEFDQTNPNPDADSTSRHFEWVYAIPFIPYLFAEPLLG
                     DLDNNALAFDIRVNTLKNYEFYGELFFDDLANPLSMFDDSWWGNKWAASIGFTAKDFC
                     TTSSICFSFNTEYTRVEPWVYTHHKGAGYTYSNYGSSLGSDLGPNSQELYTRIDAEWN
                     FLQLTLEASAVDQDTAFGGNLYDIHTPLSDTDKSFLEEESTIQYVELKSSLLVSPTDF
                     FWTRIGYSFYFGDYKGSRLEGTIGLTW"
BASE COUNT         1648 a         1001 c          978 g         1732 t
ORIGIN      
        1 gccatccatt tctacaagta actgatttaa tgtttgttct cgttcatcgt gaccaccacc
       61 aagaccagct ccacgctgac gacctacggc atcaatttca tcaataaata aaatacaagg
      121 agcgtttttc ttacccgttt caaaaagatc acggacacga gatgctccaa caccaacaaa
      181 catttcaaca aaatcagatc cagacatgct aaagaaagga acaccagctt caccagcaac
      241 agcacgagct aaaagggttt tacctgtacc tggaggcccg actaagagag ctcccttagg
      301 aatacgtcca cctaaagcat catatttttt ggggtctttt aagaatagaa cgagttcttc
      361 taaatcttgt ttcgcttcat cacaaccagc aacgtctcta aatgtggttt tatttttccc
      421 attttgaact aactgtttgg cttgactttt tccaaatgaa aacattcctt tgccgccacc
      481 actttggcga gacatcataa accagaagaa ggcaattaag ataatgatag gtaaaaaggc
      541 aattaaatga tcgacccaac ttgtcgtttc atgaatcact ttcactttta catttttgaa
      601 catttcccaa gcggtgattt gctcgtttga aatttcaagc atgtgactct taaagaatac
      661 agacggtgtt ttgtcttttt cagcttgggc aaaacgtgag aatggctttg attgagaaga
      721 ataagctttt gcttcttctg cattcatttc tcttttgcct tcaataacaa caccatcggg
      781 tgttttttga agagttaatt gagtaatgac agatgtagaa tcagccatca ttgctaaaaa
      841 ttcagttctc gaaagctctt ttgtggtttc ttcggtgtat aatcgcatca taaaaagagc
      901 gattaatatc atcgtgatta taacgaaaaa attcttattt cgaaaagggg aagctgtctt
      961 tgctttcgtc atggcgtcct ttcagtaggt tcaagttttc cggaaacagc aactccaggt
     1021 atccaaagta catctgcatt ttgaacgagt agtggtaaat aatctcgaat ccataaaggg
     1081 attccttttt cttgtagcca ctgtttcaat ttgcgctttt tgtacttaga accaggtgga
     1141 gtaaaacgat cgccttcttg acgatagcgc cattcacctg aaaagcgttt atttttttca
     1201 aaaagataca aattatcaat gttttgggca tccgccgtgc tgtaaaacca taagaaaaac
     1261 tttgattttt caatatggct ttctcgatag tggtagcgat tttgattttc tgtttgaata
     1321 aaatgcaaaa agtcatccat tggaaagtag atgcccatag aatcgagttt taatcgaaga
     1381 atttcgatta gtccgtttcc catctttgca taggcttcat tccatttttt gctagaaata
     1441 cacaaaactt ttgaataagg agagtaattt gctggaaaag gccagttttc tttagaaact
     1501 tcgatagtct taaaaaaatg atttgctttt tctaaaactt ttggatatac ctcgtggctt
     1561 aatgatgcgg tgtgagctaa aatagacatg atgcctggtg ttttcgactc taagtagggg
     1621 agaatgatta gccttgtttt gttacgtgca aaaatcgttt tctgattaga atcgtcttct
     1681 cgccatttta gttggtgatt ctttgcatat tgaattaagg aggattttgt gagatgtaaa
     1741 aagggacgaa cgactagatc atctcgtttg gctaaaatac cttgaagccc tttaagagat
     1801 gaacctcgtc gtattctcat ataaagcgtt tctgcttgat catctgcatg gtgggcggta
     1861 agaatgtaat ctagtttttg ttttttttga atatcaaata gaatttgata gcgaagctct
     1921 cttgctttgg attcaaagga ttctttgcta ttaaaaagcg aagggtctat attttgaatt
     1981 tcaaaaggaa tttgatatga ttcgcatagg cttttgacta aagaagcgtc gtcatctgcg
     2041 tttttacctc gtacgccatg atttacgtgg cagacataaa ttttagaaac attaagttct
     2101 ttgtaatggg agtaaaaata atgcaaaagg caaatggaat caacgcctcc tgagactcct
     2161 aatagaaggc tttttccata aaataattct acatctttct gaagaagcaa gagcgagctc
     2221 cagtatgaca agctacttgc ggaccttgca ttttgacctt aaaaagaagt acgtcaaaat
     2281 cacaatccac cgcccattct acaacggtca ttacgtttcc actcgtatcg cctttgtgcc
     2341 aatattcttt gcgactacga ctccaaaaga ccatttctcc acattctacg gttcttttta
     2401 aggcttcttt atccatccac gccatcatta atacatctag cttcgtagcg tcttgaacga
     2461 tcgcaggaac aaggccttgg gcatcaaatt ttatttcgga aagagtttct tcaaatgtca
     2521 tttgaacatc atccaataat taacgtttct attctcgaat ttggccgttg ccacggagta
     2581 cccatttata agtacagaga ctttctacac ccatagggcc acgagcatga agcttatcgg
     2641 tagaaatacc tacttcagcg ccaagcccat attcaccgcc atcggcaaat cgagtacttg
     2701 catttagcat cacacttgaa gagtctacat ttgcgataaa ataatcttga acttctttat
     2761 tttcagcaat aatcgcatct gtatggcggc tactatattt ctcaatatgt tcgcatgctt
     2821 cttggacatt tgcaacaaac ttaacgctca tttttaatgc aagatattca tgatgataat
     2881 tgctttcatc tccaatatct ttcacgccag aaatgcgttt ttgagcttct gaatcaccaa
     2941 acatttcgac ccctttttct cggagagggg aaatgaattg ttctactgtt ttgtcgtcta
     3001 tgtctctatg gataagcaat gtttccatgg cattgcatac gccagttctt tgtgttttcg
     3061 cattaaataa aatggccgct gtttttgaaa aattagcaga tttatctacg aaaatatggc
     3121 aaataccatt aaaatgcttg atgacaggta tacgactttg ctctacaact gctttaatta
     3181 ggttttcacc gccacgaggg ataactaaat caatcgattg attttgctgc aaaagaaaac
     3241 tcattaagga acgatcagga gtattcacta gctgaacggc attagggttg acgtggtttt
     3301 cttctaaagc acttctaaaa atgtttgcaa gttttgtaga tgtttctaat gactctttac
     3361 cacctcttag tataacggcg ttacccgatt taaagcaaag ggaagcgcca tctatggtga
     3421 catttggacg actttcataa ataaagaaaa cagagccaat aggcacagaa acacgagata
     3481 attgaattcc gttcgttaat gtattttttt ctaacacttt atttaatgga tcagcaaaag
     3541 cagctatttc ttctactcct cgagccatgc tttcaatacg agcaggattc agtgtgaggc
     3601 gatcgatcat cgcgtctgaa attactcctt ttgaatgttc cacgtcattc ttatttgctt
     3661 ttaatatgga gggtacttca ttacgtagtt tttcagcaac gcttaatagc acgctatttc
     3721 ttgtttcaat aggaagaacg cgaactgttt gagacgcgtt ccttgcatta tttgctaatt
     3781 ctaatccgta ttgttcgatc tctgtcataa gatttctctt ttttgtttac caagttaaac
     3841 caatggtgcc ttctaagcga cttcctttat aatctccaaa atagaaactg taaccgattc
     3901 ttgtccagaa aaagtccgtt ggagatacta aaagagatga ttttaattca acgtattgta
     3961 ttgtggattc ttcttctaag aaagatttgt ctgtatcact taagggagta tgtatatcat
     4021 atagatttcc accaaatgca gtatcttgat ctacagcgct tgcttctaaa gtcaactgaa
     4081 gaaaattcca ctcagcgtct atgcgagtgt atagttcttg agaattaggg cctaaatcag
     4141 agcctaatga gcttccgtaa ttggaataag tatatcctgc tcctttatgg tgggtataaa
     4201 cccaaggttc tacgcgtgta tattctgtat taaaggaaaa gcagatggaa gaagtcgtgc
     4261 aaaaatcttt tgctgtaaat ccaatagagg cagcccattt attgccccac catgagtcat
     4321 caaacataga aagagggttc gctaaatcat caaaaaaaag ctctccatag aattcataat
     4381 tttttagtgt atttacacga atatcaaaag ctaaagcgtt gttatctaaa tcgcctaata
     4441 ggggttctgc aaataaatac ggaatgaaag gaattgcata tacccattca aaatggcgag
     4501 aagtactatc agcatctggg tttggatttg tttgatcaaa ctcagtggtg gttccgtaaa
     4561 taatcatttc agaagcacct aattcgatat caaaaggtaa attgatttga agacggtgtg
     4621 catttagata tttgccagta ttttttttgt gatataactt ggttgcgtat tgagtataac
     4681 tcaatcgacc aaattcaaat tgatagccaa aatagggagt aggagctggt tcaaaaataa
     4741 aactagtcgt atccatccaa ggtctgtaaa tatgatttga tcctcttaag atcacaggat
     4801 ttcttttcgc aacaccccat tgaagataat cataaccagc taaaatagtc gtaaaaggaa
     4861 gatcgtattc aagtgaggcg cggaatcgat tccaaccctt agaattgtca gactcttttg
     4921 tgtaagggat cccatcggat ggattaaaat gattgctatt tataaaacga tttgtgtaat
     4981 ctgaaattaa ttcgttgtga ctttcaaaac gaaaagactg agagagttct cccataacag
     5041 aaagcttaaa aatagcgcta ttgtcataat agacagaact atttttataa tttgtaccgc
     5101 gtagagaatc aattaaagaa atacgaacat ttagattttt tgttcctgta gaatcactaa
     5161 atgcgagatg cccttttgct tctgttctat ttggcaaagc aaaaagtaat gtaggaatta
     5221 gggagaatat aaaaactgaa agtaaaattt tgtttttcat aataggttta ttttttgctg
     5281 aatacatttt ttaataagcg aaaatgaata ctaactcttt ctctaatgga aggaataaaa
     5341 agataaaacg gaattccta
//
LOCUS       DAOOBB010000142         5332 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_17704_length_5332_cov_3.853515, whole genome shotgun sequence.
ACCESSION   DAOOBB010000142 DAOOBB010000000
VERSION     DAOOBB010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5332
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17704_length_5332_cov_3.853515"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..1219
                     /locus_tag="PLT31_06965"
     CDS             77..1219
                     /locus_tag="PLT31_06965"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HPW94913.1"
                     /translation="MKKVSLLGATGSIGDSAIDVLKQHSDQFQLHSIAARSQWKKVYQ
                     LAKEFNIKRICMFEEQAAKELSKELGFPVLTGIDGLRELASESEVDIVINALMGAVGC
                     LPTLSAIEQGKHVALANKETMVMAGPVIQDALKNNPKSFITPIDSEHSAIFQCLSGRP
                     EKEVENLQITASGGPFREWSQDRFESITVADALNHPVWSMGKKITIDSASMMNKGLEV
                     IEAHFLFGIPYDQIKVVVHPQSMIHSLVQFRDGSLMAQLGAPDMRIPIQVALTWPDRL
                     PLNSPRLDLASLSKFTFFAPDFQKFRCLALAYEAGKSGGVVPAMMNAANEILVDQFLK
                     GNLKFVDIARHIEGIMAKAPSISGRLDLKQALEADTEARKMAASLF"
     gene            1266..2639
                     /gene="rseP"
                     /locus_tag="PLT31_06970"
     CDS             1266..2639
                     /gene="rseP"
                     /locus_tag="PLT31_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HPW94914.1"
                     /translation="MDHYLQIVLTFILGLLGLSFLVFIHELGHFLVAKKNGVRVKTFS
                     IGFGKKLLRYKKGETEYCISLIPFGGYVAMTGENPDNPDEIDEEGSFVSKSVGARAAI
                     AFAGPFVNILFAFLLLIGLYIIGVEEPVSDQLIVGFVDKTGAAQEAGILPDDTIYSID
                     GKPVKGWDDFRERIGVSLGANVTLEVHRKDSIISLSVVPKELMIPKKDSSDEMISMGI
                     GDIGIYPRHRVVIREAPVAGSNAYAAGIKMNDTILSINQQHISRYEDVIRIINASKLD
                     PLHFQLLRGEDTLNTVVNATYNKEYDRYMIGIQMVYVMYKETHIVKSSVPEAIKKASA
                     TSWKMTTSIFRYFKKMFQGQVKADAFSGPVTIVAVMGSVWMSGFQDFLMLLALISINL
                     GIMNLLPLAITDGGLLMFLAIEKVRGRPLARNTQLVIQNLAMAFFLSFFVFITFLDFN
                     KLGLFLK"
     gene            2646..3929
                     /gene="purD"
                     /locus_tag="PLT31_06975"
     CDS             2646..3929
                     /gene="purD"
                     /locus_tag="PLT31_06975"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HPW94915.1"
                     /translation="MNILVIGSGGREHAIAYAVKQSPLCQKLVCAPGNPGMSSLGECI
                     PVSVSDPKAIADLAEKEKIDLTIIGPEIPLVAGVVDEFRKRGLRAFGPIAAAAALEGS
                     KAFSKNVMKKYGIPTADYETFTDLESAQAYLRAHPAPIVVKASGLAAGKGAVVCLTDE
                     EAFQAIEEMLGEKAVFGESGKTVVIEEFMEGEEASLFAICDGKDYVLLSSAQDHKRIF
                     DNDLGPNTGGMGAYSPAPVVTESLLEDVKKRIIEPTLAGMIQEGAPYTGVLYVGIMVT
                     KNGPKVVEYNCRLGDPECQIVLPLYDGDVLALFDAAERGALSTLGAPKPPKGSAAIVV
                     LASEGYPNAYEAGQEVSGIEEAEKNGARVLHAGTKMQNEKLVTAGGRVFGVVGSGKDL
                     SEALRQAYEACEKVNFKNKYYRRDIGKKGLERCSK"
     gene            3917..4387
                     /gene="purE"
                     /locus_tag="PLT31_06980"
     CDS             3917..4387
                     /gene="purE"
                     /locus_tag="PLT31_06980"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HPW94916.1"
                     /translation="MFEIKENAKVGIVTGSASDKSIVDKVTSVLDEFGVVWEWNVLSA
                     HRTPNKTAKYAKEAEDRGVQVLIGIAGLAAALPGVLAGHSILPVIGVPGDGGPLTGVD
                     ALHSIVQMPPGIPVATVGIGNGKNAGYLAIHILALSDPSLRKKLKEYRTGLGDI"
     gene            4420..5154
                     /locus_tag="PLT31_06985"
     CDS             4420..5154
                     /locus_tag="PLT31_06985"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HPW94917.1"
                     /translation="MLLLASLIFAERGSFPWLEGEVLTYRISWGIITAGEASLETRKT
                     TGNRTEFYSLAHNNGAFKSIYPVADTIYSRIQNGSFLPEVFRKITHEGSYHNKSVIRF
                     DRAGAKAWLSDTVFSDPIKRKIKRATDTTISIFGKERCIISAFYFVRGMNFVVGEKMT
                     FSAVSGKKRYDLRVVIHGYEEINTKFGKKKCIKVEPILDGDGIFQASGRLFIWFTDDS
                     KRLPVLMKSEIIVGSIKAELIDFSQK"
BASE COUNT         1580 a          880 c         1155 g         1717 t
ORIGIN      
        1 atcgtggaga ttctttttac ttagctgctc caatgctttt cttgctatca gattttttta
       61 acgggatatt aagctaatga aaaaagtcag cttgctgggt gcgactggtt caatcggaga
      121 ttcagctatt gatgttttaa aacaacattc agatcaattt caattacact ccattgcagc
      181 tcgtagtcaa tggaaaaaag tgtaccagtt agctaaagaa tttaatataa aacgtatttg
      241 catgtttgaa gaacaggccg caaaagagtt atctaaggaa ctcggatttc ctgttttaac
      301 aggcattgat ggacttcgag aactcgcttc tgaatcagaa gtagatattg ttattaatgc
      361 tttaatggga gcagttggct gtttacctac tttgtctgca atagaacagg gtaaacatgt
      421 tgcccttgct aataaagaaa ccatggtgat ggctggccca gtcattcaag atgcgttaaa
      481 aaacaatcca aaatctttta ttactcccat tgattctgag cacagcgcta tttttcagtg
      541 cctatcaggt cgtcctgaaa aagaagtcga aaaccttcaa atcacagcat ctggaggccc
      601 ttttagagag tggtctcaag atcgttttga atccattacg gttgcggatg ccttaaatca
      661 tcctgtatgg agcatgggta aaaaaatcac tattgactct gcttctatga tgaataaagg
      721 gctagaagtg attgaagcgc acttcttatt tggcattcct tatgatcaaa taaaagttgt
      781 ggttcatcct caaagcatga tccattcttt agtgcaattt cgtgatggta gtttaatggc
      841 tcagcttggc gcccctgata tgagaattcc tattcaggtg gctttaactt ggccagatcg
      901 cttaccttta aattctcctc gtttggattt agcttcgtta tctaaattca ctttttttgc
      961 tccagatttt caaaaattcc gctgtcttgc tttagcttat gaagcaggga agagtggtgg
     1021 agtggtgcct gcgatgatga atgccgccaa tgaaattctc gtggatcaat tcttaaaagg
     1081 caatttaaag tttgtagaca ttgctcgtca tattgaaggt attatggcaa aggccccctc
     1141 tatttcaggg cgtttagatc ttaagcaagc tctcgaagcc gataccgaag caagaaaaat
     1201 ggctgcttcg cttttttaag aacacttttt gtttttagta tagttctttt ttctaaattg
     1261 tcgatatgga tcattattta caaattgttt tgacttttat tttaggcctt ttagggctca
     1321 gttttcttgt ttttattcat gaattagggc attttctagt tgcgaaaaag aatggggttc
     1381 gcgtaaaaac cttttccatt ggctttggaa aaaagctact tcgctataaa aaaggggaaa
     1441 cagaatattg tatttctcta atcccttttg gtggctatgt tgccatgaca ggtgaaaacc
     1501 ccgataatcc tgatgaaatt gatgaagagg gtagttttgt ctcaaagtct gttggagcac
     1561 gtgcagccat tgcttttgct gggccttttg tgaatatttt atttgctttt ttgttactga
     1621 tcggtcttta tatcataggg gtagaagaac ccgtatctga tcaactcatt gtcggttttg
     1681 tggataaaac aggggcagct caagaagcag gtattttacc tgatgacact atttattcta
     1741 ttgacggtaa gcctgtgaaa ggctgggatg atttccgtga acgcattgga gttagtttag
     1801 gagcaaatgt tactttagaa gtacatcgaa aagattcaat tatttctttg tctgttgttc
     1861 caaaagaact catgattccc aaaaaagatt cgtcggatga aatgatttcg atgggtatag
     1921 gagatatcgg catttatcca aggcatcgag tggtcattcg agaggctccc gtcgctggtt
     1981 ctaatgctta tgcggctggg ataaaaatga atgatactat tttgagtatt aatcaacagc
     2041 atatttcgag atatgaagat gttattcgaa tcatcaacgc ctcaaaatta gatcctttac
     2101 atttccaatt acttcgtgga gaagatactc ttaatacagt agtcaacgcg acttataaca
     2161 aagaatatga tcgttatatg attggtattc aaatggtata tgtcatgtac aaagaaacac
     2221 atatagtgaa gtctagtgta cctgaagcaa taaaaaaagc atcggctact agttggaaaa
     2281 tgacgacaag tattttccgc tattttaaga agatgttcca aggtcaagtc aaagcagatg
     2341 cattttctgg cccagtaact attgtggcag ttatgggttc tgtttggatg agcggcttcc
     2401 aagacttttt aatgttgctt gccttgatta gtatcaatct tggaattatg aatctattgc
     2461 ctctagcgat tacagatgga ggattattaa tgtttttagc gatagaaaaa gtgcgtggac
     2521 gtcctcttgc tagaaatact caattagtaa ttcaaaatct ggccatggct tttttccttt
     2581 ctttctttgt tttcattacg ttccttgact tcaacaagtt agggcttttc ttaaagtagg
     2641 gttatatgaa tattctagtt atcggaagtg gtggtagaga acatgccatt gcatatgcag
     2701 tcaaacaatc tccattatgt cagaaattag tatgtgcacc agggaatcct ggtatgtctt
     2761 ctttaggaga atgtattcca gtttctgttt ctgatccaaa agccattgct gatcttgccg
     2821 aaaaagaaaa aattgattta accattattg gtccagaaat acctcttgtt gcaggtgtcg
     2881 tggatgagtt tagaaaacgt ggcttacgcg cttttggccc aattgccgct gccgctgctt
     2941 tagaaggtag caaggctttt tccaaaaatg tgatgaaaaa atacggcatt cctactgctg
     3001 attatgaaac ttttacagat ttagaatctg ctcaggcata tttaagagct cacccagctc
     3061 caattgttgt aaaggcttct ggtttagctg caggcaaagg ggcggtggta tgtttaactg
     3121 atgaagaagc ttttcaggct atagaagaaa tgcttggaga aaaagctgtc tttggtgagt
     3181 ctggaaaaac ggtggtcatc gaagagttta tggaaggcga agaagcttct ttatttgcta
     3241 tctgtgatgg aaaagattat gtcctcttat cttcagcaca agaccacaaa cgaatttttg
     3301 ataatgattt aggccctaat acaggtggta tgggagctta ttctccagca cctgtggtaa
     3361 cggaatctct tttagaagac gtaaaaaaac gaattatcga gccaacactt gcgggcatga
     3421 ttcaagaagg agctccttat acaggtgttc tttatgtagg gattatggtc acaaaaaatg
     3481 gtccaaaagt agtcgagtat aattgccgac taggcgatcc cgaatgtcaa atagtacttc
     3541 ctctttatga tggagatgtt cttgctttat ttgatgccgc tgaacgtggt gctctttcaa
     3601 cactcggcgc acctaagcca cctaagggta gtgcggcaat agtagtactt gcaagtgaag
     3661 gctaccctaa cgcttatgaa gcgggtcagg aagtctctgg tatagaagaa gcagaaaaaa
     3721 atggggctcg agttctacat gcgggcacaa aaatgcaaaa tgaaaaatta gtcactgcag
     3781 gcggacgcgt ttttggtgtt gtcggcagtg gaaaagacct ttccgaagca cttcgtcaag
     3841 catacgaagc ctgtgaaaaa gttaatttta agaataagta ttacagaagg gatattggaa
     3901 aaaaggggtt agagagatgt tcgaaataaa agaaaacgct aaagtaggca ttgtcactgg
     3961 aagcgcttcc gataaatcga tcgtagataa agtgactagc gttttagacg aatttggtgt
     4021 cgtgtgggaa tggaatgtat taagtgctca ccgtactcct aataaaacag caaagtatgc
     4081 aaaagaagca gaagacaggg gtgttcaagt tttaataggc atcgctggtc ttgctgcggc
     4141 tcttccagga gtgctagcag ggcattctat actacctgtt attggcgttc ctggtgatgg
     4201 tggtcctctt actggagttg atgctttaca ctctatcgtt caaatgcctc caggaattcc
     4261 tgtcgctaca gtgggcattg gtaatgggaa aaatgcaggg tatcttgcta ttcatatttt
     4321 agcattatcc gatccttctc ttcgaaaaaa attaaaagaa tatcgtactg gtttaggtga
     4381 tatttaaggc tatttctttt gaaaaatatt tttttgtatt tgctgctttt agcgtcctta
     4441 atttttgcag aaaggggaag ctttccttgg ttagaagggg aagttttaac gtatcgaatt
     4501 agttggggta ttattactgc aggagaggcc tctttagaaa ccagaaaaac aacagggaat
     4561 cgaacagaat tttattccct ggcacacaat aatggtgctt tcaaatcaat atatccagta
     4621 gcagatacga tttattctcg aattcaaaac gggtcttttt tgcctgaagt gttcagaaaa
     4681 attactcatg aaggaagtta tcataacaag tcagtcatac ggtttgatcg agcaggtgca
     4741 aaagcttggc tttccgatac ggtcttttca gatcccataa aaagaaaaat aaaaagagct
     4801 actgatacca ccatctctat ttttggaaaa gaacgttgta ttatttctgc tttctatttt
     4861 gttcgaggga tgaattttgt tgttggagaa aaaatgactt tttctgcagt gagtggaaaa
     4921 aaacgttatg atttacgagt cgttattcat ggttatgaag aaattaatac gaaatttggc
     4981 aaaaaaaagt gtataaaagt tgaacctatt cttgatggag atgggatttt tcaggcatct
     5041 ggtcgattat ttatatggtt tacagacgat tctaagagat tgcctgtttt aatgaaaagt
     5101 gagattattg taggctcaat taaagcagaa ttaattgatt tcagtcaaaa ataactgaaa
     5161 aaaatacgaa aggcttctta aaaataatat tgaagaaata tttattatct tttacactcc
     5221 gttttgtaaa aaaaatgagt ataataagtt atattggtca aggttttaat cttagaggca
     5281 acaattcaat gttgaagaaa ttatttctgt tcttgatggt tttttcgttt gc
//
LOCUS       DAOOBB010000143         5299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_17826_length_5299_cov_4.482456, whole genome shotgun sequence.
ACCESSION   DAOOBB010000143 DAOOBB010000000
VERSION     DAOOBB010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 5299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5299
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17826_length_5299_cov_4.482456"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PLT31_06990"
     CDS             <1..272
                     /locus_tag="PLT31_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HPW94918.1"
                     /translation="AESWLLENTKSSPAVLIVDPPRTGLPPEALKVIAEGKIKKMIYV
                     SCNPVTLARDLFQLKNSGFEVKAVEGFAFYPHTPHLEMLLVLDRG"
     gene            328..891
                     /locus_tag="PLT31_06995"
     CDS             328..891
                     /locus_tag="PLT31_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94919.1"
                     /translation="MLKKIMFLLIVASSLALAVPAAQKSRAVGGWLQLGNAGEHFGLD
                     YKYRRTETTAIDIYGHFYFSSGDNALGVYFAHYWHNYNLLKLASDFGRIGLYAGPAGG
                     FGWWLVDHYNYRVYEYDQSGLALRFGVVGGITWEMPIPLEIYAELNPVGEFHVIFDDY
                     RDNRYDDNYVTWQIPDLYFRMGIRFWF"
     gene            912..1367
                     /locus_tag="PLT31_07000"
     CDS             912..1367
                     /locus_tag="PLT31_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94920.1"
                     /translation="MKKGTIKKISCILLILANLFLLAACEKKSVPPTIVVGREWNHQV
                     NRVADTASSFTLKDPVVFELVSETHFEFTEMECIVYEGTIKNRGPEKWRRQVKTTPNQ
                     NTYAVRGKARKGGFMSAKELFRLKKEGFVTIEFKTKDSVIAIKEIEVRK"
     gene            1367..2281
                     /locus_tag="PLT31_07005"
     CDS             1367..2281
                     /locus_tag="PLT31_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94921.1"
                     /translation="MFFVKLKQSAILFAGLSLLIGCGGSDIEVNYQLKEPVTRQLYSE
                     GYYATIDLKGVERMGTITAAYTKLKYSSHADTLSLQRTFEINKSRGYLKNYMPSELLW
                     RIREVSLKAIDRKVIETKGFELYDTLLHSLPMPVRWRNQLAHPENVKHLIRNEKHRWE
                     MSHLLKGPVPTKGNITQLLKDRGRLNFALIKIDSVVTNGFHNLDSKRCLGYMIHLKEN
                     ESFPYFIWEQHVNSKIGTEQYQSYYKGLKAEYYTQYWVAIDPETGIPCQEREFKQGVH
                     TMVNPTTGDTATFKSNITLERLYTMEKE"
     gene            2278..2721
                     /locus_tag="PLT31_07010"
     CDS             2278..2721
                     /locus_tag="PLT31_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94922.1"
                     /translation="MKWILKNYFFVLTFLCLVGCASNPSPQQTMVTDRQLVYKETYKA
                     YMEAENKYLNLLFNIERLPEEQELWMMKRDQMKELISLRELMLMSRSELDASIQDWEK
                     YLQELEQEQKKSKFISPNLKGQDVNRSSPGELLQNEAFRPPPLNH"
     gene            complement(2718..3950)
                     /locus_tag="PLT31_07015"
     CDS             complement(2718..3950)
                     /locus_tag="PLT31_07015"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HPW94923.1"
                     /translation="MINSIDSIRAQFPVLMQDSLNPKRFAFLDSTATTQKPLEVIEAM
                     NRFYKEHYSSVKRGVYRLSEKTTQAFEKTRRDVAQFIGASSENEIVFTRGTTESINLV
                     AWSYGRRFLKENDEILISALEHHANIVPWQLIAEERRAKIKVIPMDDDGNLLLDSLED
                     LIKPDVTKILALTHVSNSVGTVNPIEEIIRKARAISPSTKILIDAAQSACHLNIDVQK
                     LDCDFLAFSGHKIYGPTGIGVLYGKSDVLESMPPWHGGGEMISQVTFEKTTYAAPPAR
                     FEAGTPPIAEVIGLGAAIEWLNQVSLSKIEAHESKITQYALNALRQIPEVQFLGNPKE
                     RGALISFTLKGIHSHDAAMILDEENIAVRSGHHCAQPVMDRFGVSATLRASFGVYTET
                     WEIDRLITGLSRVIRLFR"
     gene            4011..>5299
                     /locus_tag="PLT31_07020"
     CDS             4011..>5299
                     /locus_tag="PLT31_07020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HPW94924.1"
                     /translation="MMKIIYLSVLFFGSLLFAQDILREAVDNADYDAVRQMIKKNEIE
                     EVYCGKMPPDTALQLYGKIFKAIPEQSFASCPLQFSISYGAKICSDKKQLQVCMDVIH
                     FLQMETLQGNLSAVDHFSRVTEIAIKNKAYQKPLREKVDTLVWNKCPKKQKDACIEAC
                     QLYADSTGDTLKLQECIENPVKGVIKKLTVSKPSPLNEQIKNWAIEGYWNSPVALSSL
                     WLKSILSLQEKKVIPDTLVPDMAYLTRVLTIHKEQGTVLPGKDLFRYCASWNGAVDSV
                     FMALQLDSRCPVFIGVVDARDEKVYKAIEIDGSIWIVENMNYEIEETSMCYDRESENC
                     KIYGRLYTWDAAQVACPEGYRLSKDSDWEKLETLAGGSASAANKLKSNGTDEYAFSVM
                     FGGYANKNAISTLLGEGAYFWTELETDGRAIARSIFSS"
BASE COUNT         1660 a          878 c         1077 g         1684 t
ORIGIN      
        1 acgctgaatc atggctttta gaaaacacaa aaagttctcc tgctgtttta attgtagacc
       61 ctccaagaac agggcttcct ccagaggcgt taaaagttat tgccgagggc aaaatcaaaa
      121 aaatgattta tgtttcatgt aatccagtga ctttagcgag ggatttattt caactaaaaa
      181 acagcggttt tgaagtaaaa gcagtagagg gatttgcatt ttatccgcat acacctcatt
      241 tggagatgct tttggtattg gatcgtggtt aaaaaatgtt tttttttcta gtttcaaaag
      301 gctttaattt ttaacaagag agagaatatg ctaaagaaaa tcatgttttt acttattgtg
      361 gcatcgtctt tggctttggc cgtccctgca gcacaaaaat caagagctgt tggtggctgg
      421 cttcaattag gtaatgctgg agaacatttt ggtttagatt ataaatatcg acgaactgaa
      481 acaacagcca ttgatattta tggtcatttt tatttctctt ctggtgataa tgctctaggc
      541 gtatattttg cacattattg gcacaattat aatctcctaa aactagcctc agattttggt
      601 cgcataggtc tttatgctgg ccctgcaggt ggttttggtt ggtggcttgt tgatcattat
      661 aactaccgtg tttatgaata tgatcaatct gggcttgcct tgcgatttgg tgttgttggc
      721 ggaattacct gggaaatgcc tatcccatta gaaatttatg cggaattgaa tcctgtgggt
      781 gaatttcatg tgatttttga tgattatcga gataatcgct atgatgataa ttatgtgaca
      841 tggcagattc cagacctgta ttttaggatg ggtattcgtt tttggtttta acaataatag
      901 ggaatcaaaa aatgaaaaag ggaacaataa aaaaaataag ctgtatttta cttatacttg
      961 ctaatctatt tttattggca gcttgtgaga aaaaatctgt tccaccgact attgtcgttg
     1021 gtcgagaatg gaatcaccaa gtgaatcgtg tggccgatac ggctagctca ttcactctta
     1081 aagatcctgt tgttttcgag ttagtatccg aaacgcattt tgaatttact gaaatggaat
     1141 gtatcgtcta cgaaggtact attaaaaata gagggccaga aaaatggcgc cgtcaagtta
     1201 aaaccactcc gaatcaaaat acttatgctg ttagaggcaa agctcgaaag ggcggattca
     1261 tgtcagcaaa agaattattt cggctaaaaa aagaaggttt tgtgaccatt gaatttaaaa
     1321 cgaaagattc tgtgattgct attaaagaaa ttgaggttcg taaataatgt tttttgtaaa
     1381 acttaaacag tctgctattt tgttcgctgg cctttcgctt ctgatcggtt gtggtggatc
     1441 ggatatagaa gtgaattacc agttgaaaga accagttact cgtcagcttt attcagaagg
     1501 atactatgct acgattgact taaaaggggt cgagcggatg ggtacaatta ctgccgctta
     1561 tacaaaatta aaatactcct ctcatgcaga tactttaagc cttcaacgaa catttgaaat
     1621 caataaatcg aggggctatt taaaaaacta tatgccttcc gaattactct ggcgtatccg
     1681 agaagtaagc ttaaaagcga tagaccgtaa agtcattgaa actaagggat ttgaattata
     1741 tgatacgtta cttcactctc ttccaatgcc tgtccgttgg cgtaatcaat tagctcatcc
     1801 agaaaatgta aagcacttga ttcgtaatga aaaacaccgt tgggaaatgt ctcatttatt
     1861 aaaagggcct gttcctacga aagggaacat tactcagtta ttaaaggatc gtggtcgatt
     1921 gaattttgct ttaatcaaaa tagattctgt tgtgactaat ggctttcata atttggattc
     1981 taagcgttgt ctaggttata tgattcattt aaaagagaat gaaagctttc cttactttat
     2041 ctgggaacag catgtgaata gtaagattgg gactgaacag tatcagtctt attataaagg
     2101 gttaaaagca gaatactata ctcagtattg ggtagcgata gaccctgaaa caggtattcc
     2161 ttgccaggaa cgtgaattta agcagggtgt acacacaatg gtaaatccaa cgactggaga
     2221 tacagctaca tttaagagta acattactct agaacgcttg tatacaatgg aaaaagaatg
     2281 aagtggattc taaaaaatta tttttttgta ttaacttttt tatgcttggt gggatgcgct
     2341 tctaatcctt ctccacaaca aacgatggtc acggatcgtc agcttgttta taaagagact
     2401 tataaagctt atatggaagc cgaaaataaa tacttaaatc tgcttttcaa tatagaacgc
     2461 ttgcctgagg aacaggaact ttggatgatg aagcgagatc aaatgaaaga gcttatttcg
     2521 ttacgagaac taatgcttat gtctcgttca gaactagatg cctctattca agattgggaa
     2581 aagtatcttc aggaacttga acaagaacaa aagaaatcaa aatttattag tccaaattta
     2641 aaaggacaag atgttaatcg ttcttctcct ggtgaacttc ttcaaaatga agcctttcgc
     2701 cctcctccat taaatcatta acggaataat cgaatgactc tagataggcc tgtaatcaaa
     2761 cggtctattt cccatgtctc agtgtagacg ccaaaacttg cacgtaaagt cgcagaaact
     2821 ccaaatcgat ccattacagg ttgcgcgcaa tgatgcccgc ttcttactgc aatattttct
     2881 tcatccagaa tcatagccgc atcgtgagaa tgaattcctt taagagtaaa actaattaga
     2941 gctcctcttt cttttggatt tccaaggaac tgaacttctg gtatttgacg gagtgcattt
     3001 aatgcgtatt gcgtaatttt agattcgtga gcttctattt tcgaaagaga aacttgattt
     3061 aaccattcga tggctgctcc taatccaatg acttcagcaa tagggggagt ccctgcttca
     3121 aagcgtgcgg gaggggctgc ataagtggtc ttctcaaaag tgacttgaga aatcatttct
     3181 ccaccaccat gccaaggagg catagattct agaacgtcgc ttttaccata aagaacgcca
     3241 atccccgtag gaccatatat tttgtggcca gaaaaggcaa gaaaatcaca atctagtttt
     3301 tgaacatcaa tatttaagtg gcaagcactt tgcgcggcat caattaaaat tttggtgcta
     3361 ggagaaatag ctcttgcttt tcgtatgatt tcttcaattg gatttactgt tccaacgcta
     3421 ttactcacgt gagtaagggc gagtatttta gtaacatctg gtttaattaa atcttctaaa
     3481 gaatcgagta ataaattacc atcatcatcc atcggaatga ctttgatttt ggcgcggcgt
     3541 tcttcggcaa tcaattgcca aggaacaata tttgcatgat gctctaaagc gctaattaaa
     3601 atttcatcgt tctcttttaa gaaacggcgt ccataagacc atgctacaag attgatactc
     3661 tccgttgtcc cgcgagtaaa gacaatttca ttttcacttg atgcaccaat gaattgagcg
     3721 acatctcttc ttgtcttttc aaatgcctga gttgtctttt cactaagacg ataaacccct
     3781 cttttgacgg agctgtaatg ttccttataa aagcgattca tggcttcaat cacttctaat
     3841 ggcttttgtg tagttgctgt actatctaaa aaagcaaacc tttttggatt taatgaatct
     3901 tgcattaaaa cggggaattg agcacgaata gaatctattg agttgatcat atcttttata
     3961 atagaaaaaa atagaactga tgaggttttc aattttctat attatgcgtc atgatgaaga
     4021 taatttattt aagtgtgtta ttttttggga gcctgctttt tgctcaagac atattgagag
     4081 aggctgttga taatgctgat tatgatgccg ttcggcagat gatcaaaaaa aatgaaatag
     4141 aagaagttta ttgtggaaaa atgcctccag atacggcatt gcaactgtat ggtaagatat
     4201 ttaaggcgat accagagcaa agttttgctt cttgtccact acaattctct atttcttatg
     4261 gggcaaaaat ttgttctgat aaaaaacaac ttcaagtatg tatggatgtg attcattttc
     4321 ttcaaatgga aacgttgcaa ggaaatttat cggcggtgga tcatttttct cgagtgactg
     4381 aaattgcaat aaaaaataaa gcgtatcaaa agcctttaag agaaaaggtg gataccctag
     4441 tatggaacaa gtgccctaag aagcaaaaag atgcttgcat tgaagcatgt caattatatg
     4501 cggattcaac aggagatacc ttaaaacttc aagagtgtat tgagaatcct gtaaaaggtg
     4561 ttatcaagaa gttaactgtt tcaaaaccat ctcctctaaa tgaacaaatt aaaaattggg
     4621 caatagaagg ttattggaat agtccagttg ctttgagttc actctggcta aaatcgattt
     4681 tatctttaca agagaagaaa gttattccag atacgcttgt gccagacatg gcttatttaa
     4741 caagagtatt aacgattcat aaagaacagg gaacggttct accagggaaa gatctttttc
     4801 gatattgtgc aagttggaat ggggctgtag actctgtttt tatggcttta caattagatt
     4861 ctcgttgtcc agtttttatt ggtgttgttg atgctcgtga tgaaaaagtt tataaagcga
     4921 ttgaaataga tggatccatt tggattgtag aaaatatgaa ttatgaaata gaagaaacat
     4981 ccatgtgtta tgatcgcgag agtgaaaact gtaaaatcta tggtcgtctt tatacatggg
     5041 acgctgctca agtggcttgc ccagaaggtt atcgcttatc caaagattct gattgggaaa
     5101 aattagaaac gcttgcaggt ggttctgctt ctgctgcaaa taaattaaag tccaatggaa
     5161 cagatgaata tgctttttct gttatgtttg gtggctatgc taataaaaat gctatttcca
     5221 cattacttgg agaaggcgct tatttttgga ctgaattaga aacagatgga cgtgcgatcg
     5281 caagatcaat ttttagttc
//
LOCUS       DAOOBB010000144         5218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_18179_length_5218_cov_4.136355, whole genome shotgun sequence.
ACCESSION   DAOOBB010000144 DAOOBB010000000
VERSION     DAOOBB010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18179_length_5218_cov_4.136355"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1203
                     /locus_tag="PLT31_07025"
     CDS             <1..1203
                     /locus_tag="PLT31_07025"
                     /inference="COORDINATES: protein motif:HMM:NF036710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HPW94925.1"
                     /translation="TKDGVVGYDGVANIVSGWRTRNGCPSQATTTQPKSGTTRDFWGP
                     CDEGVDMVLVTIQGKDHEPSGTSVEIWNFVKNYSLGPVGFGFKTKAKISLNPGTTQVA
                     KLETNGEGTVSFSISGGAGKDKFVINGSDLSFKEAPLYQASGSNQYAVSITAKAGDET
                     AILDMTVTILKPQTPYKGTAWAIPGKIEVEDYDVGSDENPSYSDLGGGSASEQTDYRT
                     DKVDVIKSIGNGYAVGHTQTGEWMEYTVDVKTEGVYTLEINAVSGNDGSSIRLYIGEM
                     PITENITIPNTDANYATYSVVKAKTLALSKGTHVIRLEITGNWADIDWLNFSNGTTSI
                     ENTVNYSNSGIKSYRIYNFQGAYMGTFNASDLTSLKNEIKKSNVKTGAYMIRSTDGHV
                     NKLIQIKK"
     gene            1505..3040
                     /locus_tag="PLT31_07030"
     CDS             1505..3040
                     /locus_tag="PLT31_07030"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HPW94926.1"
                     /translation="MRQFQTLMLGFCLISSVFAAPVSENIMVGSTSRNMLTHVPSGLE
                     KDSPLFISLHGMNQDAPYQQGMAHWEDIADTARFAVVYPNGINKSWDLSGNRDIDFVL
                     AIIDTMYNRHQIDRNRVYLSGFSMGGMFTYHAMGRIADKIAAFAPVSGYPMGGSAFQS
                     SRPIPIIHTHGTSDDVVGYSGVAKIVEGWRKRNNCPSQGVTTTPYPQSKPNSKSKLDF
                     WGPCDDGVNVALLTLDGKGHWHSNDGAVGVHTSHEIWNFVKNYSLTGSSIVVVPSPRD
                     SIFNGKFDQGKSAWTLNVWDGEATGTIDNEEYKIDVSSIGTENYQIQLIQSGLILEKD
                     QSYKLTFDARATANRSIEINVEQHEDPWASYLESVQNFDLTITKNTYSFVFTMNAATD
                     QNGRLSFNLGGATGTVFLDNVVIEKTETPPTVISEKTPLNQTKISYSHGNLFVKFDAQ
                     KNKKVQLLVYNLQGVEVMSKVLFAKDGIEQRMNFSTLSAGRYLVQVRSGNSIMKSMNI
                     LHY"
     gene            3094..>5218
                     /locus_tag="PLT31_07035"
     CDS             3094..>5218
                     /locus_tag="PLT31_07035"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HPW94927.1"
                     /translation="MKNIKMWAFFLAIACYVQGATVSNPIIWADVPDPSMLRVDDTYY
                     MVSTTMHYAPGVPIMASTDLANWRTINYAYSTLTNSNAMNLNAGASAYGKGSWASSLR
                     YNKGTYYILTPSYTTNKTHLYQTTDIKNGPWKETLFNFYHDPSLFFDDDGKVYIIYGG
                     GEFKIIELNSDLSGVKQGGVNQTLKIPLDATTGTSSYIVKMEGAHMEKVNGKYYLFAI
                     TWPSGNGRTELVFRSSSITGNYEGKIALQNNGVAQGSIFDTPDGKWYAVLFRDNGAVG
                     RIPYLIPVTWSNDWPVFGNAAPATLELPNPVYPGFNMVTSDDFDEETLPLEWQWNHNP
                     DNANWSLKAKPGSMRITTSRVDSRLYYAKNTLTQRSFGPKCSGRIAVDVSGMKDGDVA
                     GLVALQDSLAFVGVAKSGSSLSVVQYQGTSQKASVPISQNKVYFRIDMDFTNQTDKAY
                     FFYSLDSLNWKTIGGTLSMRYTLGMFVGYRFGLFNYATKSAGGYVDFDWFKIGATVDQ
                     EIKLPPLVFEEIPQEPYKGVLSIPGAIEAENYDLGGHNNAYSDNDYDNQGGEYREDGV
                     DVVTNDNGYAVGYTEAGEWLEYTVNVEAEDVYTVEARVASGSETSSFRLFIDDEAITD
                     TIKVETTGDWDTYSVITAKTSKLKKGSQILKIAVTGSYVNIDHIKFSQGTIGISKPLH
                     LNTNRGMQEYSVYHFNGALLGTYKAS"
BASE COUNT         1619 a          844 c         1141 g         1614 t
ORIGIN      
        1 actaaagatg gtgttgtggg ctatgacgga gtcgctaata ttgtctcagg atggcgtaca
       61 cgaaatggtt gtccaagcca agccacaacg acacaaccta agtctggtac gacacgcgac
      121 ttctggggcc catgcgatga aggggtggac atggttttag tgacgataca aggaaaagac
      181 catgaacctt ctggtacgag tgttgaaatt tggaactttg taaaaaatta ttccttgggt
      241 cctgtaggat ttggttttaa gacaaaagca aaaatctctt tgaatcctgg cacaactcaa
      301 gtggcgaaac ttgaaaccaa tggcgaaggg acagtttctt tttccattag tggtggcgct
      361 ggtaaagata agtttgtcat caatggaagc gacttgagct ttaaagaggc tcctctctat
      421 caagcgagcg gctccaatca atacgcggtc tccattacag caaaagcggg tgatgaaaca
      481 gcgatattag atatgacagt gactatttta aaaccgcaaa cgccttacaa gggcactgct
      541 tgggctatac ctggcaagat cgaagtggaa gactatgatg tgggttctga tgaaaaccca
      601 tcatattccg acttaggcgg cggatctgct tcagaacaaa cggattatcg tactgataaa
      661 gtcgatgtaa tcaagagcat tggaaatggc tacgctgttg ggcatacaca aactggtgaa
      721 tggatggagt atacggtcga tgtgaaaaca gaaggagtgt atactctaga aattaatgct
      781 gtttctggaa atgatggttc ttctatccgc ttgtatattg gtgaaatgcc gattacagag
      841 aatatcacga ttccaaatac ggatgctaat tatgcaactt atagcgtggt taaggcgaaa
      901 acattagcgc tctccaaggg gactcatgtg attcgactcg aaatcacagg aaactgggct
      961 gatattgatt ggttgaattt ttctaacgga actacatcta ttgaaaatac agtaaattac
     1021 agtaattcag gtataaagag ctatcgcatt tataatttcc aaggtgctta tatgggaaca
     1081 tttaatgcct ctgatttgac ttcattgaaa aatgaaatca aaaaatcaaa tgtaaaaact
     1141 ggcgcttata tgattcgttc aacagacggt catgtgaata aattaattca aattaagaaa
     1201 taataagttc tgattttcta ttaataaaat aatagatgca aatcaaaata atccttctct
     1261 tcactcgaga aggatttttt ttgcttttat aacccttttt cagattattc aaaacaaccc
     1321 aattttggtc aaattcttga attttaagtc aaaatggtgt ggactattta tcaatacaaa
     1381 agactagaat gcaagcgaaa acggactaat taagaaatag atttatgtat gcttctagac
     1441 ataggctcat gtttttaaac aagtaaaact tgaggattgg ttttgttttt ataaggagtg
     1501 ttgtatgagg caatttcaaa cattaatgct tggtttttgt ttaatctcaa gtgtatttgc
     1561 agcccctgtt tcagaaaata ttatggtggg ttcaacttca cgtaatatgt tgacgcatgt
     1621 gccatcaggt ttagaaaaag attctccatt gtttatttct ttacatggaa tgaatcaaga
     1681 tgctccttat caacaaggaa tggctcattg ggaagatata gctgatacgg ctcgttttgc
     1741 agtggtgtat cctaatggga ttaataaatc atgggatctc tcaggtaatc gagatattga
     1801 ttttgtcctt gctattattg atacgatgta taatcgtcat cagattgatc gcaatagagt
     1861 atatctttct ggattttcga tgggaggaat gtttacttat catgcaatgg gacgtattgc
     1921 cgataagatt gcagcctttg ctccagtctc agggtatcca atgggaggta gtgcttttca
     1981 gagttctcgt ccaatcccca ttattcatac acatggaacc tctgatgatg tagtaggtta
     2041 tagcggtgtc gctaagattg ttgagggatg gcgtaaaaga aataattgcc caagtcaagg
     2101 tgtcacaact acgccttatc cacaatctaa accaaattca aaatcaaaat tggatttttg
     2161 gggcccttgt gatgacggcg tgaatgtcgc tttacttact cttgatggaa agggacactg
     2221 gcattctaat gatggcgctg ttggagtaca tacgagtcat gaaatttgga attttgttaa
     2281 aaactattct ttaacaggat cttccattgt agttgttcct tctccaagag atagcatctt
     2341 taatggcaaa tttgatcaag gaaagtccgc ttggacgtta aacgtatggg atggtgaagc
     2401 cacaggaacc attgataatg aagaatataa aatcgatgtt tctagcattg gtacagaaaa
     2461 ttatcagatt cagttgattc aaagtggttt gattttagaa aaagatcagt cttataaatt
     2521 gacttttgat gctcgtgcta cagcaaatcg ttccattgaa ataaatgtag aacagcatga
     2581 agacccttgg gcaagttatt tagaatcagt tcagaatttt gatttgacta tcacgaaaaa
     2641 cacgtattca tttgtcttta cgatgaatgc agccacagat caaaatggtc gtttgagctt
     2701 taatttaggt ggtgctacag gaaccgtatt cttggataat gtagtcattg aaaaaacaga
     2761 aactcctcca acagtgatta gtgaaaaaac gcctctgaat cagacgaaga tttcttattc
     2821 acatggaaac ttgtttgtga aatttgatgc acagaaaaat aaaaaagtgc agttactggt
     2881 ttacaattta cagggtgtag aagtgatgtc gaaagtgtta ttcgcaaaag atgggattga
     2941 acagcgaatg aatttttcaa cactttctgc aggccgttat ttagttcaag tacgttcagg
     3001 aaattcaatc atgaagtcta tgaatatttt gcactattaa aaagtttgtt taataaatgg
     3061 gattgttgaa taaataaaac gtgggattgt tttatgaaaa atataaagat gtgggcgttt
     3121 ttcctcgcca ttgcttgcta cgtgcaagga gcaacggttt ctaatccaat tatttgggct
     3181 gatgttcctg atccatcaat gcttcgtgtt gatgatactt actatatggt cagtacgaca
     3241 atgcattatg cgccaggtgt gcctatcatg gcgtctacgg atcttgctaa ttggcgaacg
     3301 attaattatg cgtattcgac attaacaaat agtaatgcga tgaatttgaa tgcaggcgcg
     3361 agtgcgtatg gaaaaggttc atgggcttcg agcttgcgtt ataataaagg gacttattac
     3421 attttaacgc cttcttatac aacaaacaaa acgcatttat atcaaactac cgatattaaa
     3481 aacggacctt ggaaagagac gttgtttaat ttttatcatg acccctcttt gttttttgat
     3541 gatgacggga aggtctatat aatttatggt ggtggtgagt ttaaaattat cgaattaaat
     3601 tccgatttat ctggtgtaaa acagggtggt gtaaaccaaa ccttgaagat tcctttagat
     3661 gcgaccacag gaacatcctc ttatatagtc aagatggaag gggcgcatat ggaaaaggtc
     3721 aatgggaagt attatctatt tgccattact tggccgagtg gaaatggacg aacagaacta
     3781 gtttttagat cttcctcaat tactggaaac tatgagggga aaattgctct tcaaaataac
     3841 ggcgtggctc aagggagtat ttttgatacg cctgatggaa aatggtatgc ggtgctgttt
     3901 agagataatg gtgctgtagg acgtattcca tatttaattc ctgtgacttg gtcaaatgat
     3961 tggcctgtat ttggaaatgc ggctccagca acattagaac ttccaaatcc tgtctatcct
     4021 ggctttaata tggtgacttc agatgatttt gatgaagaaa ctttgccatt agaatggcaa
     4081 tggaatcata atccagataa cgccaattgg tctttgaaag caaaaccagg ctctatgcgc
     4141 ataactactt ctagagtgga tagccgtctt tactatgcta aaaatactct aacgcaaaga
     4201 tcttttggcc ctaaatgttc tggtcgaatt gctgtagatg taagtggaat gaaagatggt
     4261 gatgtcgctg gattagtggc tcttcaagac tcgctcgctt ttgtcggtgt agcaaaatct
     4321 ggatcttcac tttccgttgt tcaatatcaa ggaacgtcac aaaaagcaag tgttcctatt
     4381 tctcaaaata aagtgtattt ccgaatcgat atggatttca cgaatcaaac ggataaagcc
     4441 tatttctttt atagcttaga tagtttgaat tggaaaacga taggcggcac tctttcaatg
     4501 agatatacgc taggtatgtt tgtagggtat cgttttgggt tatttaatta cgctacaaaa
     4561 agtgctggtg gctatgtgga ttttgattgg tttaaaattg gtgccaccgt tgatcaagaa
     4621 attaagcttc ctcctcttgt ttttgaagaa attcctcaag agccttataa aggggttctc
     4681 tccattccag gagcaattga agccgaaaat tatgatttag gtgggcataa taacgcttat
     4741 tctgataatg attacgataa tcaaggcgga gagtatcgtg aagatggcgt tgatgtggtg
     4801 acaaatgata atggttatgc ggtgggatac actgaagcag gtgaatggct tgaatacacc
     4861 gtgaatgtag aagcagaaga tgtctataca gttgaagctc gagtcgcttc tggaagtgaa
     4921 acttcttcat ttaggctctt tattgatgac gaagcaatta ctgatacgat caaagtagag
     4981 actactggcg attgggatac ttatagtgtt ataactgcaa aaacgtcaaa acttaagaag
     5041 ggtagtcaaa tcttgaaaat tgctgtcact ggtagttatg tgaatattga tcacatcaag
     5101 ttttcacagg gaaccattgg catttcaaaa ccgcttcatt taaatacaaa tagaggaatg
     5161 caagaataca gtgtttatca ttttaatggt gcgcttcttg gtacatataa agctagtg
//
LOCUS       DAOOBB010000145         5209 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_18207_length_5209_cov_4.500000, whole genome shotgun sequence.
ACCESSION   DAOOBB010000145 DAOOBB010000000
VERSION     DAOOBB010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18207_length_5209_cov_4.500000"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..1257)
                     /locus_tag="PLT31_07040"
     CDS             complement(64..1257)
                     /locus_tag="PLT31_07040"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HPW94928.1"
                     /translation="MKVIAPNGDKLNLMPLNVGPSHGATHGCLRYFCALDGESIVATV
                     GEIGYLHRGFEKMAERGTWQQVVPYTDRLNYCSAMMNNIAYCRAVENMFQMELPERTK
                     ILRVIINELSRMADHCICVAAAAQDLGSTTGFFYLFDSREMMMVIWEKLTGARLTNSY
                     SRIGGLYRDTYDGFEEDVLAVCKQAEKNLRDVHACFDRNRIFLDRTVGIGAMKPERAI
                     QWGWTGPCLRATGVASDLRKDEPYYDYETYDWNVVTGTAGDVYDRLMVRLAEIEESIK
                     IVRQALKRLTPGPIDIMDPRIRVPSHALAYQDIESLIGRFKSIYEGVRVPAGEYYCGS
                     EAANGELGFTLVSDGSGKPWRVKVRPPCFTQFAAFQELVEGSMLTDSVATLSSINIIA
                     GELDR"
     gene            complement(1254..1859)
                     /locus_tag="PLT31_07045"
     CDS             complement(1254..1859)
                     /locus_tag="PLT31_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HPW94929.1"
                     /translation="MNFFETLFPVLETKFNVEKDASAHWDHSVIAKASVLRDLITFLK
                     NDESSKMEMLLDIVGIDYLGFQDYEGPRFAVEYVLKSISHPGRRVRVKVFVSEQDLEV
                     PTISDLYPIANWQEREVYDQYGILFKDHPDLRRILNHVEFEGHPLRKDYPAHRRQWLS
                     TNDFLLPALEKRLESKGYKVVQHSKEVLPNEDEYLTGSIQE"
     gene            complement(2041..2499)
                     /gene="nuoB"
                     /locus_tag="PLT31_07050"
                     /pseudo
     CDS             complement(2041..2499)
                     /gene="nuoB"
                     /locus_tag="PLT31_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
     gene            complement(2735..2947)
                     /locus_tag="PLT31_07055"
     CDS             complement(2735..2947)
                     /locus_tag="PLT31_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94930.1"
                     /translation="MLVKIWTDSFIITGDIDTLRDERLTDYIRENKDFIAVTQVRVTD
                     RNGKDLFRTHFLNVSTNHIEIILPAE"
     gene            3042..3881
                     /locus_tag="PLT31_07060"
     CDS             3042..3881
                     /locus_tag="PLT31_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HPW94931.1"
                     /translation="MKKAKTTPVKTKKKAVVTRPEPLISYYDKNFLESTAGRQVRMLS
                     EFIAPGVTFQEEKIKNTIVFFGSARTLSMSEIQKQRKKTKDPKKLARLKHLENVAEYY
                     DLARELAKRLGKWMNHPRHESYAICTGGGPGIMEAGNRGADDMGSPSIGLNIKLPFEQ
                     HSNPYITDCLNLQFRYFFIRKYWFLNLARALVAFPGGFGTLDELFEILTLIQTQKYAQ
                     KMPVVIFGTKFWKKLVNWDYLVETGMIDKEDLSLFKFCDSVDEAYDYITSILEKQDGV
                     KKD"
     gene            4126..>5209
                     /locus_tag="PLT31_07065"
     CDS             4126..>5209
                     /locus_tag="PLT31_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPW94932.1"
                     /translation="MIKNYIAECLDRISRNLALREHYTMAEYQRWFDQNPEYLHNGAV
                     EQIKLIEPLTLEGTNDLYRANYWIERPNDKMVYGNREIVVKICKYWTPPGKNRLHRLN
                     MLMSAFQDEIRINNLIRATNIEGVVQSYGGGLAGRHPYLKMEFIKGCSLDQTFKEKLT
                     DEELIARIAQIAYLANSISQLHYYQIIHKDLKPKNLLLCLNPDHINNHKILICDFGYA
                     QAKLRESVTEYGGQLTPCYSAPEQAVMGENLSPSVDYFSFGIILHEYLTGKNLFPKAM
                     DIFIEDEYQITERYLEYMQKGKENIFSHPKFPEITQWINDLTIFDSYERMQKSENLFN
                     IAHKLREKVNALGYRDVNTDFLWNQLR"
BASE COUNT         1726 a         1043 c          890 g         1550 t
ORIGIN      
        1 aaggacctct ttcaaaactc agcttattgt tggcaacaaa atgaacagca ccttgaactt
       61 ttttcatcga tccaattctc ccgcaataat gttaatacta gaaagagtag cgacagagtc
      121 agttaacata gaaccttcga caagctcttg aaatgcggca aattgagtaa agcaaggtgg
      181 acgcactttc acacgccagg gtttccctga accatctgaa accaatgtaa agcctagttc
      241 accattcgct gcttcggagc cacaataata ttctcctgca ggcacacgaa cgccttcata
      301 aatactctta aaacgcccaa tcaaggattc aatatcttga taagctaaag catgtgaagg
      361 cacacgaata cgaggatcca taatatcgat tggacctgga gtcaaacgtt ttaaagcctg
      421 acgaacaatt ttaatagact cttcaatttc agctaagcga accattaaac gatcataaac
      481 atcaccagca gtacctgtaa cgacgttcca atcataggtt tcataatcat aataaggctc
      541 gtctttacga agatcactag caacaccagt tgctctgagg caaggaccag tccaacccca
      601 ttgaatggct ctttctggtt tcatggcacc aataccaacc gttctatcta agaaaatacg
      661 gttgcgatca aagcaagcat gaacatcacg aagatttttt tctgcttgct tacaaacagc
      721 aagaacatct tcttcaaagc catcataagt gtctctataa agaccaccaa tacgagaata
      781 actattagtt aaacgagcgc cagtgagctt ttcccaaatg accatcatca tttcacggct
      841 atcaaacaaa tagaagaagc ccgtggtaga acctaaatcc tgagcagctg cagccacaca
      901 aatacagtgg tcagccatac gggataattc attgatgatg acgcgtaaaa ttttggtacg
      961 ttctggtagt tccatttgga acatgttttc tacagcacga caatacgcaa tattattcat
     1021 catcgcagaa caatagttta agcggtctgt ataaggaacc acttgttgcc aagtgccgcg
     1081 ttcagccatt ttttcaaagc cacgatgcaa ataaccaatt tcacccactg tggctacaat
     1141 ggattcgcca tctaaggcac aaaaataacg aagacacccg tgagtggcac cgtgagaagg
     1201 ccctacgttt aagggcatta aattaagctt atcgccatta ggagctataa ctttcattct
     1261 tgaatactcc ctgtcaaata ttcatcttca ttaggcaaca cttctttaga atgttgaacc
     1321 actttatacc cttttgactc taagcgcttt tctaatgctg ggagtaaaaa gtcgtttgtt
     1381 gaaagccatt gacggcggtg cgctgggtaa tccttacgaa gaggatggcc ttcaaattct
     1441 acatgattta atatacgacg aagatcagga tgatccttaa agagaatgcc atactgatca
     1501 tagacttcac gttcctgcca atttgcaatt ggatacaaat cagaaatggt tggcacttcg
     1561 aggtcttgtt cgctgacaaa aacttttact cggacccgac gtcctggatg agaaatactt
     1621 tttaatacgt attcaacggc aaagcgagga ccttcgtaat cttgaaagcc caaatagtcg
     1681 atgccgacta tatccaaaag catttccatt ttagaggatt cgtcattttt taagaaagta
     1741 atcaagtctc ttaatacaga ggctttagca atgaccgaat gatcccaatg agcagaagca
     1801 tctttctcta cattaaattt ggtttccaaa acaggaaaaa gcgtttcaaa aaagttcatt
     1861 ttaacgcccc tttttaaatg gaaatacatc taatacatcg tttactttag aacgtaccgt
     1921 attagatacc atttcacaag cttcattttt acgaagaata agatcttcac gacactcttt
     1981 agacttgcgt ttaatatctt caatctggtc tttaagacgt tctgcacgtt gcttcatgta
     2041 agattcatct tccacttttt tctgcaagtc aaacatcgca tcaaagaaag actctgggcg
     2101 tggaggacat ccaccgatat aaacatcgac agggatgata tgatctatgc ctggaacggt
     2161 gcaatagcaa tcgtaaaaac cgccagagca agcacaagca cccatagcaa tcacccaacg
     2221 aggttctgcc atttgctcgt aaatacgttt taaaatagga gcttgcttat aggtaatggt
     2281 acctgcaact aatagcacat ctgactgacg tggagtaaaa cgtacatatt cagaacctat
     2341 tcgcgataaa tcataacgag ccgtttccgt gctcataaat tcaattgcac aacatgccgt
     2401 accataaggg aacggccaaa gcgaattttt gcgtccccaa ttgacaagaa agtcaagtga
     2461 agatgtaaaa atattgcctt ctcgggcctc tggtgtcata aacggttacc tcataaaatt
     2521 catgctgaaa aatagaaaga tccttctttt ttaacagcta aaaaaaacga aattcaattg
     2581 tttttcaaag agaattcccc tgttttacag aattttatag gattttttat acaaaaaata
     2641 accgtaaggc aaaaagcctt actttttttt agcgactgct aagccaaaga ttcctcccaa
     2701 aatggaactc taaaaatttt cttagaagga ctctttattc cgcaggtaaa atgatttcta
     2761 tatgattcgt actcacattt aaaaaatgcg tacgaaacaa atcctttcca tttcgatcag
     2821 ttacccgcac ttgtgtcact gcaataaagt ctttattttc acggatataa tcagtcaaac
     2881 gttcatctcg taacgtatcg atatctcccg tgataataaa actatctgtc catattttaa
     2941 caagcatgtc gtaaatataa agtttttttg cttttcaaaa tgagaaaacg agcttttttt
     3001 ctaaaaaacc ttatatttat ccttatctta aaggattttc tatgaaaaaa gcaaaaacaa
     3061 cccctgtaaa aacgaaaaaa aaagctgtag tcactagacc agagcctctt atttcttatt
     3121 atgacaaaaa ctttttagaa agtactgcag gtcgtcaagt acgaatgctc tctgaattta
     3181 ttgctcctgg agtcactttt caagaagaaa aaattaaaaa caccattgtc ttctttggat
     3241 ctgcaagaac gttgtccatg agcgaaattc aaaagcaaag aaaaaagaca aaagacccca
     3301 aaaaactagc tcgattaaaa caccttgaga atgtcgctga atactatgat cttgcaagag
     3361 aactagccaa aagacttggt aaatggatga accaccctcg tcacgaaagc tatgctattt
     3421 gcactggtgg cggacctggc attatggaag caggaaatcg aggtgcagat gacatgggtt
     3481 ctccatccat cggcttaaat atcaaattac cttttgaaca acattctaat ccttatatca
     3541 cagattgtct taatttacaa ttccgctatt tctttattag aaaatattgg tttttgaatt
     3601 tagcaagagc cttagttgct tttcctggtg gctttggtac cttggacgaa ttatttgaaa
     3661 tactcacttt gattcaaacg cagaaatacg ctcaaaaaat gcctgttgtc atttttggaa
     3721 cgaaattctg gaaaaaatta gtgaactggg attatctcgt ggaaacagga atgattgata
     3781 aagaagactt gtctctattt aaattctgtg attctgtaga tgaggcctat gattatatta
     3841 cttcgattct tgaaaaacaa gacggagtaa aaaaggattg attttttaag caaaaattct
     3901 ttcacccttt ttcaaaagcg acaaggaatt ctttgccgct tttttttgaa acttatttcc
     3961 ccttttttca tttttatgaa aatcaaatta taataattga ttttattttc tatttaaatg
     4021 aagagaattc tctattttat tactcttttt ttcaagatgt tatttattca ctacaaaaag
     4081 agaatccttc ttcttcttct ttcattttaa tgctacattt aacgcgtgat aaagaattat
     4141 atagcagaat gcttagatcg tatttcacgt aaccttgctt tgcgtgaaca ctacacaatg
     4201 gcagaatatc aacgctggtt tgatcagaat cctgagtacc ttcataatgg agccgtagaa
     4261 caaattaaat taatagaacc tttaacactt gaagggacta atgatttata tagagcaaat
     4321 tattggatag agcgtcccaa tgataaaatg gtctatggca atcgcgaaat tgtagtcaaa
     4381 atttgtaaat actggactcc tccaggaaaa aatcgtttac accgattaaa catgttgatg
     4441 agtgctttcc aagatgagat tcgcatcaac aacctaattc gtgccaccaa tatcgaagga
     4501 gtggttcaaa gttatggcgg aggtctcgcg gggcgtcatc cttacttgaa aatggaattt
     4561 attaaagggt gttctttaga tcagactttt aaggaaaagt taaccgacga agaactcatt
     4621 gctcgcattg ctcaaatagc ttatcttgct aattccatta gccaattgca ttattatcaa
     4681 atcattcata aagatttaaa acctaaaaat cttttactct gcctaaaccc agatcatatt
     4741 aataaccata aaattttaat ttgtgatttt ggttatgcac aagccaaact ccgtgaaagc
     4801 gtcactgaat atggtgggca actcacccca tgttatagtg caccagaaca agctgttatg
     4861 ggcgaaaacc tttctccttc tgtagattat tttagttttg gtatcatctt gcacgaatat
     4921 cttactggaa aaaatctttt tcctaaggct atggatatct ttatagaaga tgaatatcaa
     4981 attacggaac gctacctaga gtatatgcaa aaagggaaag agaatatttt ttctcacccc
     5041 aaatttccag aaattacgca gtggattaac gatctcacaa tatttgatag ttatgaacgg
     5101 atgcaaaaaa gtgaaaacct ttttaacatc gctcataaac ttagagaaaa agtaaatgct
     5161 ttaggttatc gtgatgttaa cactgatttt ctatggaatc aattgcgcg
//
LOCUS       DAOOBB010000146         5048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_18882_length_5048_cov_3.821150, whole genome shotgun sequence.
ACCESSION   DAOOBB010000146 DAOOBB010000000
VERSION     DAOOBB010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 5048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5048
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18882_length_5048_cov_3.821150"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PLT31_07070"
     CDS             complement(<1..223)
                     /locus_tag="PLT31_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94933.1"
                     /translation="MKKDGIDKVLIIGSGPIVIGQACEFDYSGTQACKALREQGYKIV
                     LVNSNPATIMTDPVMADATYIEPLNVGRLE"
     gene            complement(382..>1684)
                     /gene="ilvC"
                     /locus_tag="PLT31_07075"
     CDS             complement(382..>1684)
                     /gene="ilvC"
                     /locus_tag="PLT31_07075"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HPW94934.1"
                     /translation="SGLDVSYTLRAEAIAEKRASWKNATENGFKVGTYEEMLPDADLV
                     CNLTPDKQHHSVIPVIMPLMKKGAVLSYSHGFNIVEEGQQIRPDITVIMVAPKGPGSE
                     VRAEYVRGFGMPALIAVNRENDPEGKGWDYAKAYAVGLGSDRAGVLESSFIAEVKSDL
                     MGEQTILCGMLQTGTLLCFDKMVADGVDKAYASKLLQYGWETITEALKHGGITNMMDR
                     LSNPAKIRAYELSEQMKKIMAPLYQKHQDDIISGHFSSTMMKDWEASDKDLLTWREET
                     GKTEFEKTAATDKAISEQEYFDRGVLLVAMVKAGVELAFETMVSVGIKPMSAYYESLH
                     ETPLIANLIARKKLYEMNRVISDTAEYGCYLFANACVPLLKDFMKTVNKDDIGAVYGA
                     EKTTSVDNMRLISVNAAIRKHPVEEIGAWLRSKMRGMTKIV"
     assembly_gap    1685..1694
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1695..1865)
                     /locus_tag="PLT31_07080"
     CDS             complement(<1695..1865)
                     /locus_tag="PLT31_07080"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820155.1"
                     /note="catalyzes the formation of
                     (R)-2,3-dihydroxy-3-methylbutanoate from
                     (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and
                     isoleucine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HPW94935.1"
                     /translation="MNYFNTLPLRIQLEELGHCRFMDSCEFSRGVEALKGKKIVFVGC
                     GAQGLHQGLNLRD"
     gene            2015..3523
                     /locus_tag="PLT31_07085"
     CDS             2015..3523
                     /locus_tag="PLT31_07085"
                     /inference="COORDINATES: protein motif:HMM:NF003716.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HPW94936.1"
                     /translation="MNFTPENILLAGSILLFISIFASKAGGKIGVPALLLFLGVGMIG
                     GVDGFGIEFNNPHVSQFIGVMALSVILFSGGMDTRFSEIKPVIAQGVTLATFGVVSTA
                     LVTGVFIHYLSNFIDGFISLGWVESFLLASIMSSTDSASVFSILRSKGLQLKHNVRPL
                     LELESGSNDPMAYMLMIFFMTILQANQASVPLSVLNVVIQLVVGLVAGYFLGQLTRTV
                     INKINLVNDALYPVLLFAFVFFTFTVTDLLHGNGYLAVYIAGMVVGNSKLLHKKNMMT
                     FFDGFTWLWQIIIFLALGLLVKPTELFTTPVAGAGLLIGAFMIVVARPVSVFLCLAPF
                     LKIPFKVRLYVSWIGLRGAVPIIFATYPLIAGIPNADLFFNLVFFITIISLLTQGTTV
                     TLMAKILGISEAEKEKANMFGIELPEEIKSAMSEFTVIPSVLHNGNRLMDIILPDNTL
                     VVMIKRGHQFFVPKGNTTLAVGDKLVLISDNEAELYRSYSKLGVTNYSVKKN"
     gene            complement(3529..>5048)
                     /locus_tag="PLT31_07090"
     CDS             complement(3529..>5048)
                     /locus_tag="PLT31_07090"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HPW94937.1"
                     /translation="LCGLLIYVLTKTPEAAGQGTDNMIYAFHNQGGKIRKRVAPVKFF
                     SSIITLGSGGSAGYEGPISQIGSGIASAIGSIFKMPRSLRGQFTLAGTAAGLGAIFKA
                     PLAGALTSVEVLYKEDFEASAFATSIVAAVVSFTVYTASVGLEPTFPGMPDFSFINGK
                     ELLVCALLGFFCVPFSLLYVWAYNESEKRFARLEIPLFLKPALGGLFVGLLFLAYPEI
                     TGGGFGFISEMILNQKTVAGFGIVILIAVAIAKIIGTAFTVGSGGSGGVFGPSIFIRA
                     VLGGAFAGLVEWFLPGTLREPEAMILIGMGTFFAGTSKASIAGVVMVCEMTGSYSLLP
                     GLLIASVMHIALSRRWTIYKSQVKNKFASPVHRAEMDPDVLRVITVGEVMVKKEIQNL
                     QASDVLNEVKSLMDSKHSFPVLDHGKYIGLLDMNAARYHMIHTPALSYNVLVSDCTIK
                     VPLLNRSLDLHSAMRLFLRLGTTEFYVKDKTGQIVGLLSYASILKAYDQLVSPKK"
BASE COUNT         1556 a         1089 c          878 g         1515 t
ORIGIN      
        1 cttctaaacg gcctacattc aatggttcga tataggtggc atcagccatc acgggatccg
       61 tcattatggt agcaggatta gaattcacta atacaatctt ataaccctgt tcacgaagtg
      121 ccttacatgc ttgtgttcca gaatagtcaa actcacaggc ttgacctatg acgattggac
      181 ctgaaccgat tatcaaaact ttatcgatgc catctttttt catttttttt tgtctccagg
      241 ataaaacgac aaatttctaa aagccaaatt ttggcaaata tagttttttt acaccgatgt
      301 cccaatgcat aaaaagagaa aagcaaaaaa aacaccgctc gagagagcgg tgttaaaatt
      361 ctaatgatta aaacaaagaa attaaacgat tttagtcatg ccacgcattt tagaacgtaa
      421 ccatgcacca atttcttcaa caggatgttt tctaatagca gcatttacag aaattaagcg
      481 catattatca acacttgttg ttttctcagc accataaacg gcgccaatat catctttatt
      541 cactgttttc atgaaatctt ttagaagagg tacgcaagca tttgcaaata agtagcaacc
      601 atactcagca gtatcactaa tcactcgatt catttcataa agtttcttac gagcgatcaa
      661 gtttgcaatc agtggtgttt catgtaaaga ttcgtagtaa gcgctcattg gcttaatacc
      721 gacagagacc atggtttcaa aagcaagttc cacaccagcc ttaaccatcg ctacgagtaa
      781 aacgccgcga tcaaaatatt cttgttcaga aatagcctta tctgttgcag ctgttttctc
      841 aaattctgtt ttaccagttt cttcacgcca ggtcaaaaga tccttatcag aagcttccca
      901 gtctttcatc atagtagaag agaagtgacc agaaatgata tcatcttgat gcttttggta
      961 taaaggagcc atgatctttt tcatctgttc ggaaagttca taagctctga ttttcgctgg
     1021 attagataag cgatccatca tgttggtgat accaccatgc tttaaagctt ctgtaatagt
     1081 ttcccaacca tattgaagaa gtttagatgc ataagcttta tctacgccat cagccaccat
     1141 tttatcaaag caaagcaatg tacctgtttg aagcatacca caaagaatgg tttgttcccc
     1201 catcaagtca ctcttcactt cagcaatgaa ggaactttcg agaacgcctg cacggtcaga
     1261 acctaaaccg acggcataag ccttagcata atcccatccc ttgccttctg ggtcgttttc
     1321 acgattcaca gcaataagag ctggcatacc aaaaccacgg acgtattcag cacgaacttc
     1381 agagcctggc cctttaggtg caaccatgat cacggtaata tctggacgaa tttgttgacc
     1441 ttcttccaca atgttaaaac cgtggctata agaaaggaca gcacccttct tcattaaagg
     1501 cataataact ggaataacag aatgatgttg tttatctggt gttaaattgc acacgagatc
     1561 ggcatctgga agcatttctt cataagttcc gaccttaaag ccattttcag ttgcattttt
     1621 ccaagaggca cgtttttcag caatggcctc tgcacgaagt gtataggaaa cgtcaagacc
     1681 actgnnnnnn nnnngtctct caaatttaaa ccttgatgta aaccttgagc accacagccc
     1741 acaaatacaa tcttctttcc ttttaatgct tctacgcctc tagaaaactc acaggagtcc
     1801 atgaaacggc aatgacctaa ttcttcaagc tggatacgaa gcggaagcgt attaaaataa
     1861 ttcatttgaa atcctctttt gataaacaat gccaggtaat ttagaaaaaa aatgtatttc
     1921 ttatatccat tttttaaaga atgcacaata aaaggtcttt ttttttagtt acatttaaaa
     1981 aaattcgttt ttttcgcgtt tttacaaagg tatcatgaat tttacaccag aaaatatcct
     2041 tctagcaggc tccattctat tatttattag catttttgcg agtaaagcgg gcggtaaaat
     2101 tggcgttcct gcccttctcc ttttcttagg cgtagggatg attgggggtg tagatggctt
     2161 tggaattgag ttcaataacc ctcatgtatc gcaatttatt ggtgtcatgg ctctaagtgt
     2221 gattctattt tctggaggca tggacactcg attttctgag atcaagccag tcattgctca
     2281 aggagtcact ctggccacat ttggtgtggt gagcactgcc ctagtcacag gggttttcat
     2341 acactacttg tctaacttta ttgacggttt tatctcttta ggttgggtgg aatcattctt
     2401 actagcatcg attatgtctt ctactgattc tgcctcggta ttctctattc ttcgatctaa
     2461 aggtcttcaa ttaaagcaca atgtacgccc tcttcttgag cttgaaagcg gtagtaatga
     2521 tcctatggcc tatatgctaa tgatcttttt tatgaccatt ttacaagcaa atcaagcaag
     2581 tgttcctctt tccgttttga atgtagtgat tcaattagta gtaggccttg tcgctggtta
     2641 tttcctcggg caattgactc gaaccgtaat caataaaata aaccttgtaa atgacgcttt
     2701 ataccctgtt ttactttttg cttttgtctt tttcaccttt acagttaccg atcttctcca
     2761 tgggaatggt tatcttgccg tgtacattgc tggtatggtc gttgggaata gtaagctttt
     2821 acacaaaaaa aatatgatga ccttttttga tggttttaca tggctatggc aaatcatcat
     2881 ctttttagca ttaggcctac ttgtgaagcc aacagagctt ttcaccactc cagtagctgg
     2941 tgctggtctc ttgatcggtg cctttatgat tgtagtcgct cgccctgttt ctgtattttt
     3001 gtgcttagcc cccttcctaa aaataccatt taaagtaagg ctttatgttt catggattgg
     3061 tttacgtggt gccgtcccta ttatttttgc cacttaccca ctgatagcag gcattcctaa
     3121 tgcagactta tttttcaacc tagtcttttt catcacgatt atttctcttt taactcaagg
     3181 gacgacagtc acattaatgg ctaaaatact tggtatttca gaagcagaaa aagaaaaagc
     3241 aaacatgttt ggcattgaac tccctgaaga aattaaatca gccatgtctg aatttacagt
     3301 aatcccttct gttttacata atgggaatcg cttaatggat atcatactac cagataatac
     3361 tctcgtagtg atgatcaaac gaggtcatca attttttgtg cctaaaggaa ataccactct
     3421 tgctgtcgga gataaactcg ttttaatttc tgataatgaa gccgaattat atagatctta
     3481 tagcaaactc ggcgtcacca attattccgt aaagaagaat tagtttttct atttctttgg
     3541 gctaaccaat tgatcatacg cttttaaaat agaagcatac gaaagtaatc ctacaatctg
     3601 acccgtttta tcttttacat aaaactctgt ggtaccgagc ctcaaaaaaa gcctcatcgc
     3661 ggagtgtaga tctaaagacc gatttaatag aggaactttg atagtacaat cagagaccag
     3721 aacattataa gaaagagcag gggtgtgaat catatgataa cgagccgcat tcatatctaa
     3781 taatccaata tatttaccat gatctagtac aggaaaggaa tgtttagaat ccattaatga
     3841 tttaacttcg tttaaaacgt cactcgcttg taagttttgt atttcttttt tcaccatcac
     3901 ttcacctaca gtaatgacac gcaagacatc gggatccatt tcagctcgat gcacaggaga
     3961 agcaaactta tttttaacct gacttttata aatagtccaa cggcgtgata aagcaatatg
     4021 catcacagat gcgattaaaa gccctggaag caagctgtaa ctccctgtca tttcacaaac
     4081 catcaccacc cctgcaatag atgcttttga ggtaccagca aaaaaagtac ccatcccaat
     4141 caaaatcatg gcttctggtt cacgaagcgt cccaggtaga aaccattcca caagaccagc
     4201 aaaagcacct cccaaaacag ccctaataaa tatcgatgga ccaaaaacac ctccagagcc
     4261 accactacct acagtaaaag ctgtccctat tatttttgca atcgctacag caattaaaat
     4321 gactattcca aatccagcca ccgttttttg attcaagatc atttcactaa taaaaccaaa
     4381 cccacccccc gtaatttctg ggtaagctaa aaataaaagc cctacaaaaa ggcctcccaa
     4441 agcaggtttt aaaaataaag gtatttctaa acgagcaaac cttttttcag attcattata
     4501 agcccataca tataataaag aaaagggaac acaaaagaag ccaagtaaag cgcaaacgag
     4561 gagttctttt ccattaataa aggaaaaatc aggcattcca ggaaatgttg gttcaagacc
     4621 aaccgaagca gtataaacag taaaagaaac tacagcagcc acaatggaag tggcaaaggc
     4681 gctcgcctca aaatcctctt tatataagac ttcaacagat gtcaatgcgc ctgctaaagg
     4741 ggccttaaag atagcaccta accctgcggc agtacctgca agcgtaaatt ggcctcgtaa
     4801 agaacgaggc attttaaaaa tagaccctat tgcagaagcg atcccacttc caatttgaga
     4861 aatagggcct tcataaccag cactcccccc cgaccctaga gtaataatac tagaaaaaaa
     4921 cttaacggga gcgactcgct tgcgtatttt cccaccctga ttatggaacg cataaatcat
     4981 gttgtctgtt ccctgccccg ctgcctcagg tgtttttgtt aaaacataaa tcaaaagacc
     5041 acataata
//
LOCUS       DAOOBB010000147         4968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_19258_length_4968_cov_4.371260, whole genome shotgun sequence.
ACCESSION   DAOOBB010000147 DAOOBB010000000
VERSION     DAOOBB010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19258_length_4968_cov_4.371260"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="PLT31_07095"
     CDS             <1..510
                     /locus_tag="PLT31_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94938.1"
                     /translation="DGEEEQNPKAQVPTRFMSWQDAIFLLVIAGLVVGGYYYFQYTKQ
                     KGTKQFAECQKLFEANDLLASEVCYEKTWELSYVTDSMELDRQHYLGLISDKRTVQMD
                     VFQLVEASFLEGDSAKAFEEMTKMSEPLLLLDQDQIDLWKEWSKKSAIKAAISAATPI
                     SVTDSSQKQ"
     gene            complement(476..898)
                     /gene="tsaE"
                     /locus_tag="PLT31_07100"
     CDS             complement(476..898)
                     /gene="tsaE"
                     /locus_tag="PLT31_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPW94939.1"
                     /translation="MIVQNENETLLWAEQFAKTLKPGAVVAISGNLGAGKTVISRGIC
                     KALGFKGIVCSPTYTIVHEYPNTPPLYHLDLYRLNYIQDLEEIGFDHLISGEGITLIE
                     WPERLRENTLGITHWVNVLILSETQRKITVSEKNPLQK"
     gene            complement(895..2022)
                     /gene="nspC"
                     /locus_tag="PLT31_07105"
     CDS             complement(895..2022)
                     /gene="nspC"
                     /locus_tag="PLT31_07105"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HPW94940.1"
                     /translation="MIDYSLVPSPCFVLEETRLRRNLEILNYVQKKSGAKIICALKGF
                     SMWSTFPIVGEYLPGATASSLNEALLAKNEMRKEVHVFAPLYEDGEIDQILSLADHIS
                     FNSFSQWERFKNKVLKTNVSPGIRVNPESSSVETDLYNPCGRFSRLGVTLKEFKAEAL
                     EGIEGFHFHALCEQNVDALETVLQAFEEKFGRWIPQMKWVNFGGGHHITRKDYEVDKL
                     VQLIISFKKRYPGIEVILEPGEAVGWQTGELVATVGDIVYNETDIAILNISVSAHMPD
                     CLEMPYRPMVRGSGIAQEKKFTYKLTGNTCLAGDVIGDYSFDEPLKVGDRLIFEDMIH
                     YTMVKTTFFNGVRHPDIGIWTKENQFHLVRHFTYEQFRDKL"
     gene            2160..2912
                     /locus_tag="PLT31_07110"
     CDS             2160..2912
                     /locus_tag="PLT31_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94941.1"
                     /translation="MTLFLFLGYVTAFSADTSSVVPSVSDDSLNRGISTDSALTSSRG
                     SNASMEDLIQAKLDSLNNLSESQTGTARNLDSIAKAKSDSIRVLMTEGKYIQRVKYDK
                     SNFDHWKIDTVFQKQMKGHLSGSWRTPIVPHGHSFRDSELRFLDNDTLYGVTRTYSDS
                     GRYQMTGEYAFYARYRFENDSTIVSREVFSDRDVVRWDFIYFKINGDQLTYHLKKLEF
                     RDLNDNWLNALQGFDHVSPEIYYRHKKDEKKK"
     gene            2914..3888
                     /locus_tag="PLT31_07115"
     CDS             2914..3888
                     /locus_tag="PLT31_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPW94942.1"
                     /translation="MSLYLRLFFLLTLVVLLSGLTMILGPASFSFKDLFFSSELDPFA
                     LNVLFELRLPRVFAAVFAGTALSVAGLSLQSVFRNPLAGPYVLGISSGASLGVALVLL
                     AGFSFGSLGFFPAAALGAFAVTFLVLMFSRLFENSAMLLVVGLMISYIVDACITLLMH
                     FSEAEALRSYVAWGMGSFSRLTVDGFLAFFIAVVLGLSFILFSMRYLNAVRLGDDFVK
                     GLGLSVRLYQTLVLLGTSILTAATTVFCGPIAFLGLAVPHIAFALFKTSNHRILIPAS
                     ILIGINLSLCVGLLPQIPLNAMMSLFGVPVVFWVLFSSRYKRKGDTYG"
     gene            3881..4603
                     /locus_tag="PLT31_07120"
     CDS             3881..4603
                     /locus_tag="PLT31_07120"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW94943.1"
                     /translation="MAEEIVLSVKELSVGYQKSLTTSFDLNCFSGELICMLGPNGTGK
                     STLLKTLAGLVVPLSGSIDLLGKSISKYSLKERAQKLTTVFMAHLLTPEMTVREYVSL
                     GRTPYAGFLDARSEKDLQVVQNAIEDTRLSSFADNPMYALSDGERSRVFIARALAQEV
                     KVLLLDEPTAFLDVPHTIFLFDLLKKITRLKNNTIILSTHNIEYAKRFADRIIAFDGK
                     GNVSLMDVKSAFESDFLQWTEG"
     gene            4603..>4968
                     /locus_tag="PLT31_07125"
     CDS             4603..>4968
                     /locus_tag="PLT31_07125"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HPW94944.1"
                     /translation="MYKIIFLLCLFFLNTGCTKSQITEQESTKHFLWKVSDADSYVWI
                     LGSIHMADSTFYPLDPVIMNAFNESQALVLEIDISNDSVLNESSSLSMDRGVLPSGQL
                     LKDVLPDSTYMHLDSLFRSW"
BASE COUNT         1403 a          866 c          925 g         1774 t
ORIGIN      
        1 gatggagaag aagaacaaaa tcccaaagct caagtgccta ctcgttttat gagttggcaa
       61 gacgccattt ttcttcttgt tatagcaggc cttgtggttg gtggttatta ttattttcaa
      121 tacaccaaac aaaaagggac taagcaattt gctgagtgcc aaaaattatt tgaggcaaat
      181 gatcttttag ctagtgaagt ctgctatgaa aaaacatggg aattgtctta cgttacagat
      241 tctatggagt tagatcgcca gcattattta ggtttaatta gcgataaaag aacagttcaa
      301 atggatgtct ttcaacttgt agaagcatct tttttagaag gtgattctgc taaagctttt
      361 gaagaaatga caaaaatgtc agaaccatta cttcttttgg atcaagacca aatagatctt
      421 tggaaagagt ggtcaaaaaa gagcgccata aaagctgcta tttccgctgc tacacctatt
      481 tctgtaacgg attcttctca gaaacagtaa tttttctttg agtttcggag aggataagaa
      541 cattcaccca gtgtgttata cctagtgtgt tttctcttag tctttcaggc cattctatta
      601 gtgtgatgcc ttctccagat attagatgat caaaaccgat ttcttctaaa tcttgaatat
      661 aattgagtct atataaatca agatgataga gaggtggagt atttggatat tcatgaacaa
      721 tggtatatgt aggggagcaa acaatcccct taaatccaag tgctttgcaa atgcctctcg
      781 aaatcactgt tttaccagcg ccgagattcc ctgagatggc aacaactgct cctggtttca
      841 atgtttttgc aaattgttct gcccatagaa gtgtttcatt ttcattttga acaatcatag
      901 tttatctcta aactgttcgt aagtaaaatg acgaactaaa tgaaattgat tttcttttgt
      961 ccaaatgcct atatcaggat gtcggacacc attaaagaat gtcgtcttta ccatggtata
     1021 gtgaatcata tcttcaaata ttaaacgatc acctactttt aatggttcgt cgaaggaata
     1081 gtctccaatc acgtctccag caaggcaggt gtttccagtt aatttgtatg taaacttttt
     1141 ttcttgagcg atcccactcc cgcgaaccat tggacggtag ggcatttcaa gacagtcagg
     1201 catatgagca cttaccgaaa tatttaaaat ggcaatatct gtttcattat aaacaatatc
     1261 gccaaccgtc gctacgagtt ctcctgtttg ccaaccaacg gcctctccag gttcgagtat
     1321 tacttcgata ccaggatacc tttttttgaa tgatataatc aattgaacta atttatcgac
     1381 ttcataatct tttcttgtga tatgatgtcc gccaccaaaa ttcacccatt tcatttgagg
     1441 aatccatcgg ccaaattttt cttcaaaggc ctgtaaaacg gtttctaaag catcgacatt
     1501 ttgttcgcac aaagcgtgaa aatgaaagcc ttcgattcct tctagagctt cagccttaaa
     1561 ttcttttaat gtgacaccaa gtcttgaaaa acgaccacaa gggttgtaga gatcggtttc
     1621 aacactggat gactctgggt ttactcgaat accaggactc acattcgttt ttaaaacttt
     1681 atttttgaaa cgttcccatt gagaaaaact gttaaaggaa atatgatcag ctagagataa
     1741 aatttgatct atttctccgt cttcataaag aggagcgaaa acatgaactt ctttacgcat
     1801 ttcgtttttt gcaagaagcg cttcgtttaa actggaggct gtggctccag gaagatattc
     1861 tccaacgatg ggaaaagtgc tccacatact aaaacctttt agagcacaaa taattttggc
     1921 ccctgatttt ttttgcacat aattgaggat ttcaagatta cggcgtaatc gagtttcttc
     1981 taacacaaaa caggggcttg gaactaaact ataatcaatc atagcgcaaa tataaaacct
     2041 aacatcaata agctcaaatt ttaggctttt tttttggatc ttttttcaaa aaacattatt
     2101 cttttacttc ccattagtta aatttaacag tgatgaaatt cttttttaat tcatattttt
     2161 tgacactctt tctctttttg ggttatgtaa ctgctttctc agcagacact tcttctgttg
     2221 ttccttcagt ttctgatgat tctctcaaca gaggtatttc tactgattct gctttgacct
     2281 cttcacgtgg ttctaacgca tccatggaag atttgattca agcaaaattg gattctttga
     2341 ataatttgtc tgagtctcaa acaggtacag ctcgtaattt agatagcatc gcaaaagcta
     2401 aatccgatag tattcgtgtt cttatgactg agggcaaata catccagcgg gtgaagtacg
     2461 ataaaagtaa tttcgaccat tggaaaatcg acaccgtatt tcagaagcaa atgaaaggtc
     2521 atttgtctgg ttcttggcgt actcctatag tacctcatgg tcattccttc cgagattcag
     2581 aacttcgttt cttagataat gatacgcttt acggagtcac tcgtacctac tctgattctg
     2641 gtcgttatca gatgactggt gagtatgctt tttatgctcg ctatcgtttt gaaaatgatt
     2701 ctaccattgt cagtagagaa gttttttcag atcgtgacgt agtgcgttgg gattttattt
     2761 acttcaaaat aaatggtgat caattaactt atcatcttaa aaagcttgag tttagggatc
     2821 tcaatgataa ctggttaaat gctcttcaag gctttgatca tgtttcgcct gagatttatt
     2881 atcgccataa aaaggatgaa aaaaagaaat agtatgagcc tttatcttag gcttttcttt
     2941 cttcttactt tagtcgtttt actgtcgggg ctgactatga ttcttggtcc agcctctttt
     3001 tcttttaagg atttattttt ttcaagtgaa ttagatccgt ttgccttaaa tgttttattt
     3061 gaattacgct tacctagagt tttcgctgct gtctttgctg gaacggctct atctgtagcg
     3121 gggctttctc tacaaagtgt ttttagaaat cccttagcag gtccttatgt attaggaatt
     3181 agttctggag ccagtttggg agtggctcta gttttacttg caggtttttc ttttggctct
     3241 ctaggctttt ttcctgcagc cgctttaggt gcttttgccg tgactttttt agtgttgatg
     3301 ttttcacggt tatttgaaaa ttctgcaatg cttttagttg tgggtttaat gattagttat
     3361 atcgtagatg cttgtattac attgttaatg cattttagtg aagcagaagc gctacgaagt
     3421 tatgtggctt ggggcatggg ctcgttttca agattaacag tagatggatt tcttgctttt
     3481 tttattgctg tagttttagg cttatcattc attctttttt ctatgcgcta tttaaatgca
     3541 gttcgcttag gagacgactt tgttaagggc cttggtttat ccgttcgttt atatcaaaca
     3601 ctcgttctct taggcacatc tattctcaca gcagctacta ctgtcttttg tggcccgata
     3661 gcatttttag gactagctgt tccacatatt gcttttgctc ttttcaaaac aagtaatcat
     3721 cgcattttaa taccagcatc catattaatt ggaataaatc tttctttatg tgtaggacta
     3781 cttcctcaaa tacctttaaa tgcaatgatg agtttatttg gtgtgcctgt tgttttttgg
     3841 gttttatttt cctctcgtta taaaagaaag ggagatactt atggctgaag aaattgtctt
     3901 aagtgtaaaa gaactcagtg taggttacca aaaatcgtta accacttctt ttgatttgaa
     3961 ttgtttttct ggtgagctta tttgtatgct tggaccaaat ggtacaggaa aaagtacttt
     4021 attaaaaaca ctcgctggat tagttgttcc tctttctggc tcaatagatc ttttaggaaa
     4081 atccatttca aaatattcat taaaagaacg agctcaaaag ttaacgactg tttttatggc
     4141 acatttatta actcctgaga tgactgttag agaatatgtc tcattgggac gtacgcctta
     4201 tgcaggtttt ttagatgcaa gaagtgagaa ggatttgcaa gtggttcaaa atgccataga
     4261 ggatactcgt ttatccagtt ttgcagataa tccaatgtat gctttaagtg atggagaacg
     4321 ttctcgtgtt tttattgcta gagctcttgc acaagaagtt aaagtattgt tgttagatga
     4381 accgactgct tttttagatg tacctcatac gattttttta ttcgatttac taaaaaaaat
     4441 aactcgttta aaaaacaata ccattatttt aagcacacat aatattgaat atgcgaaacg
     4501 ttttgccgat cgaatcatcg cttttgacgg taaaggaaac gtttctttaa tggatgtaaa
     4561 aagtgctttt gaaagtgatt ttttacagtg gacggaaggc taatgtataa aattattttt
     4621 ttactatgtc tcttttttct taatacggga tgtactaaat cacaaatcac agaacaagaa
     4681 tccacgaagc attttttatg gaaagtttca gatgccgatt cttatgtatg gattttaggc
     4741 agtattcata tggcggattc tacgttttat cctttagatc cagtgattat gaatgctttt
     4801 aatgaatcac aagcacttgt tcttgaaata gatatttcaa atgatagtgt attaaatgaa
     4861 tcatcctctc tttcgatgga taggggagtt ttgccatcgg gtcagttatt aaaggatgtt
     4921 cttcctgatt ctacgtacat gcatttagat tctttatttc gttcttgg
//
LOCUS       DAOOBB010000148         4908 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_19544_length_4908_cov_5.034824, whole genome shotgun sequence.
ACCESSION   DAOOBB010000148 DAOOBB010000000
VERSION     DAOOBB010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 4908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4908
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19544_length_4908_cov_5.034824"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            434..2362
                     /locus_tag="PLT31_07130"
     CDS             434..2362
                     /locus_tag="PLT31_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPW94945.1"
                     /translation="MQSIRNIAILAHVDAGKTTLSERILFTAGEIGRPGNVEEGLATM
                     DYLPEEKARGITIEAGVAHFEWRNIWFNFIDTPGHIDFGAEVDMALSAVEGAVLVVSA
                     ANGVETQTMTAWKKLRSAGVRTLLFINKLDNQDYSLDEVLLDVEERLGVRPLLLSLPE
                     YTKGKMSSVIDVISQTRLKHNEDGREEAVPLSSKKASSEALKYYKEAVEFASLFDDPI
                     LEQAIKGEDVSPKDLIRGLENLAKSDEYVLCYAGSALENFGVRSLMTGLSFFLPSPPS
                     FHKKTLGQVVRLRFFKGYGEISLFRSMCNLQSSKWPLGFSFYRMKANMLVPEEVIREG
                     DIYAMKSEEKMELGNYLGKDGKIFALETEEPIRNKYLPLLQTQIECLKIEDFNHIEES
                     LQILSRMDPSFKLARHHEGGYWILHTVGEVQLDVLLARLKREFHCDVRAGEPEVQWQE
                     RLVDKVGPIDNSFQVGPHKVSVSISAEPLLDSESDIRLRADFLEEAPREILAGVRSAL
                     LETAEVGFLGKGSLVGVLFEIHSLEYTQDVNISMIKKACADAVLLLLKKAGFVLYEPY
                     MALHLECPVEFAGAVTNDIQSRQGKIKEIAGDGKTHSLEAEVPLKEFFGYSTAVRSIS
                     KGTAVYDLHLVGFKKRIC"
     gene            2526..4394
                     /locus_tag="PLT31_07135"
     CDS             2526..4394
                     /locus_tag="PLT31_07135"
                     /inference="COORDINATES: protein motif:HMM:NF014237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HPW94946.1"
                     /translation="MFTAGAFIVLAATSAIAAPTVYEAEAQADVVTDSIREGAEYSGG
                     KYVSVFKAMDFKVTVEETAVYDITTRVLIKQYDWTTSKIQVNGTDVGSMLTTPRNCDS
                     SFVVTASAKMRVGENTITVGNGAIGVDYISVEKHPDPVFNLNATPVTPNASQNAYKLK
                     AFLTENFGKKTISGMMISDQVFNYDYANMKIIPPGSSCTDSCDYGDDQVIWKGQTDIA
                     EFYKRSGHYPAIGGFDMLFAAGGYSEDGWFRGYSENNLLMAESLWNMGGIPTFTWHWK
                     VGEDTVFYTQGSPPGFNNSGCTEGVMGTGEKNTCFNYTKAFKDASCKEINAASQEYQD
                     IINDIDKVSVLFKQLQEKDVAVIWRPLHEASGGWFWWGVASGDCYKQLYRLVFDRMVN
                     VNKLNNLLWVWNINTDPKFGYDYSALNAAWYPGDEYVDIVAVDIYDQLLNHNSAVNYY
                     NKIVSDVGTDKIIALSENGAIPDIDSIAEDKAYWSYWMTWSQTWSGNFLDKTPTDMWV
                     RNLNDERIIALDDMPGWDNVKVGIPKRKITNGDASVTNKLDIAVINKTLTLHLPQYGR
                     GSVALFDMQGHQIATLVSGNITAGTKRFDLQSIASGSYLVRVNLNGMNKMQRVTLK"
BASE COUNT         1443 a          933 c         1106 g         1426 t
ORIGIN      
        1 tattctcacg aaaataatgt ttcattgaat tgggtctgtc agggatttaa ttgcccgata
       61 aatttgtttc tagtcgaatt aggttaataa actgcgtaat ccgtcgttgt tcaggcgttc
      121 gctgtacttg ttgtatcgtt ttttagcctt tatgcttttg gtaagcttat tctcacgaaa
      181 ataatgtttc attgaattgg gtctgtcagg gatttaattg cctgatgatg ttatttcaag
      241 tcgaattacg ttaataaact tcgtaatccg tcgttattca ggccttcgcc gtactatttg
      301 tacggcttta gccttcacgc ctcggctagt ttatgacaag ctcatcttta gctgaatagt
      361 ttgttttacg tggaaaaggg ttgttgtaag agaataatta atttattaag gtattctttt
      421 ctaaattaag caaatgcaat ccattcgtaa catcgcgatt cttgctcatg tagatgcagg
      481 taaaactacg ctatcggagc gtattctttt tactgctggc gaaattgggc ggccagggaa
      541 tgtagaagaa ggccttgcaa caatggatta tttacccgaa gaaaaagctc gtggtattac
      601 gattgaagct ggtgttgctc attttgagtg gcgtaatatt tggtttaact tcattgatac
      661 tcctggacat attgattttg gtgctgaagt agatatggct ttgtcggctg tggaaggtgc
      721 tgtacttgtg gtttctgcag caaatggtgt tgaaactcag acgatgacgg cttggaaaaa
      781 actgagatca gccggagttc gtactctttt atttattaat aaactagaca atcaggacta
      841 ttcactagac gaagttttgc ttgatgttga agaacgtttg ggtgtgcggc ctttgttgtt
      901 aagtcttcca gaatatacaa aaggcaaaat gagctccgtc attgatgtca ttagtcaaac
      961 acgtctaaaa cataatgaag atggtaggga agaagctgtt cctttgagtt ctaaaaaagc
     1021 atcttctgaa gctctcaagt actataagga agcggttgag tttgcttcgc tttttgatga
     1081 tcctatttta gaacaagcca taaaagggga agatgtttct ccaaaagatt tgattcgagg
     1141 tttagaaaac cttgcaaaat cagacgagta tgttttgtgc tatgctggtt ctgcgcttga
     1201 aaattttggt gtccgaagtt taatgactgg tctttctttc tttttacctt ctcctccttc
     1261 ttttcataaa aagactttag gacaagtggt tcgacttcgc ttttttaagg ggtatggtga
     1321 gatttctttg tttagaagca tgtgtaatct acaaagttct aaatggcctc ttggcttttc
     1381 attttatcga atgaaagcaa atatgcttgt tccagaagag gttattcgtg aaggcgatat
     1441 ctacgccatg aaaagtgaag aaaaaatgga gttaggaaat tatttaggca aggatggaaa
     1501 gatctttgct ttagagactg aagaaccgat aagaaataaa tatttacctt tgcttcaaac
     1561 tcaaatagag tgtttgaaaa tagaagattt taatcatatt gaagaaagtc tgcagatctt
     1621 atcaagaatg gacccttctt ttaagttagc tcgccatcat gaagggggat attggattct
     1681 tcacacagtg ggagaagttc aattagatgt gttattagct cgtttaaaga gggagtttca
     1741 ttgtgatgtt cgggcagggg agcctgaagt acaatggcaa gaacgtcttg tggataaagt
     1801 ggggccaata gataattctt ttcaagtggg gccacataaa gtgagcgttt ctatttcggc
     1861 agaaccatta ctagatagtg agtcggatat tcgtttacga gccgattttt tagaagaagc
     1921 gcctagagaa atcttggcag gagtaagatc agcattatta gaaacggccg aggtgggctt
     1981 tttaggaaaa ggatctttag taggagttct ttttgaaata cactctcttg agtatactca
     2041 agatgtcaat atttctatga taaaaaaagc ttgtgcggac gccgttttat tacttttaaa
     2101 aaaagcgggt tttgtgcttt atgagccgta tatggctttg catttagagt gtcctgtaga
     2161 atttgcagga gctgtcacga atgatattca gtcgagacaa ggaaaaataa aagaaattgc
     2221 gggtgatggg aaaacgcact ctttagaagc cgaagtaccc ttaaaagaat tttttggcta
     2281 ttctacagcg gttcgttcta tttcaaaagg aacagctgtt tatgacttgc acttagtcgg
     2341 atttaagaaa cggatttgtt gaaatgtctg tttagggttt ctgaaaataa taactaaaaa
     2401 acggcacaat tcgtttcgat atctaaaatg gtgaagtttt acttaattac tgttggataa
     2461 gacactctac cccaagttaa ataaaaaaag taaattaaaa aacgcgcgga ttgtcgcgca
     2521 aaaaaatgtt cactgcaggt gcttttattg tactcgctgc aacaagtgcc atcgccgccc
     2581 cgacggtata cgaagccgag gcacaagcag atgttgtcac tgattctatt agagagggcg
     2641 ccgaatattc gggcggcaag tacgtctccg ttttcaaggc catggacttc aaggttacgg
     2701 tcgaagaaac cgcggtctac gatattacaa cgagggttct catcaagcag tacgactgga
     2761 cgacttccaa aattcaggta aacggaaccg atgtaggatc aatgctgact actccacgca
     2821 attgcgattc tagtttcgtg gttacagctt ctgcaaagat gcgcgttgga gaaaatacca
     2881 tcacggtcgg caatggcgcc attggcgttg actacatttc ggtcgaaaaa caccccgacc
     2941 ctgtattcaa ccttaatgcc acgcccgtaa cgccgaacgc ctcccaaaat gcttataagc
     3001 taaaggcctt tttaaccgaa aactttggca aaaagaccat cagcggtatg atgattagcg
     3061 atcaggtatt taactacgat tacgccaaca tgaaaatcat tccgcctggt agtagctgca
     3121 cagactcctg tgactatggc gatgatcagg tcatctggaa gggccaaacc gatattgctg
     3181 aattttacaa acgcagtggt cactacccag ccattggcgg ctttgacatg ctctttgccg
     3241 ctggcggcta ctccgaagac ggttggttca gaggttattc tgaaaataac ctgctgatgg
     3301 ccgaaagtct gtggaatatg ggcggcattc ctacatttac atggcactgg aaggtgggcg
     3361 aagatactgt tttctacacg caaggaagcc cacctggttt taacaattct ggctgtaccg
     3421 agggcgtcat gggaacaggc gaaaagaaca cttgctttaa ctacaccaaa gcatttaagg
     3481 atgcctcctg caaggaaatc aacgccgctt cgcaggaata ccaggacatc ataaacgaca
     3541 tcgacaaggt ttctgtactc ttcaagcagc tgcaagaaaa ggacgtcgct gtcatctggc
     3601 gcccgcttca cgaagccagt ggcggttggt tctggtgggg cgttgccagt ggcgattgct
     3661 ataagcagct ctatcgtctc gtatttgatc gcatggtgaa tgtcaataaa ctcaacaacc
     3721 tgctttgggt ctggaatatc aataccgacc ctaaattcgg ttacgactac tcggctctca
     3781 acgccgcctg gtaccctggt gacgaatatg ttgatatcgt agcagtcgat atttacgacc
     3841 agctccttaa ccacaattcc gcagtcaatt actacaacaa gattgtgagc gacgtgggca
     3901 ccgataaaat tatcgccctc agcgaaaatg gagccattcc cgacatcgat agcattgccg
     3961 aagacaaggc ctattggagc tactggatga cttggagtca aacctggagt ggaaacttcc
     4021 tcgataagac tcctaccgac atgtgggtgc gaaacctcaa tgacgaacgc atcatcgctc
     4081 ttgacgacat gccaggctgg gataatgtta aagtgggtat tccaaaaaga aagatcacca
     4141 acggagatgc tagcgttaca aataaattag atattgccgt tatcaacaag actcttactt
     4201 tgcacttgcc gcaatatggc cgcggttctg tcgctctgtt tgacatgcaa ggccaccaaa
     4261 tcgccacact tgtgagcgga aacatcactg caggcactaa gcgctttgat ctgcaatcaa
     4321 tcgcgagcgg tagctacctc gtgcgtgtaa atctcaatgg tatgaataag atgcagcgag
     4381 tgacactgaa gtagtaatta cggaaaatac aatataaaac cccgcccttt acggacgggg
     4441 ttttgcgtta aagcttatct gtctgacatt ttctttgcct tttctattga cccatttgtt
     4501 atagatttgt ttgagattga ctagtgtaac gcagagaaaa acgcctatcc aagtgttcag
     4561 tatatatttg gtaaagtatc gcttaccctt gaaaacactg cttttatgag tagttttgaa
     4621 aaaataattg taaaaaaaat aacactttct cccttaaaaa cctcaaaatt aatcgtttag
     4681 aataaaaaat ttgtaaaaaa taaaagaatg cttgccaaat tctgtaaaat tgctaaaatt
     4741 gaggcctcaa attcgaggag tatcgcttcc tatgcgcctc gaactaaata agtccttaaa
     4801 aagtaaggat ttacaagtac tgaagcgata aaccttctgg tgaagaagag gaaaaaatgg
     4861 caaaagaaaa gtttgataga agcaagccgc actgcaacat cggcacca
//
LOCUS       DAOOBB010000149         4901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_19595_length_4901_cov_4.892076, whole genome shotgun sequence.
ACCESSION   DAOOBB010000149 DAOOBB010000000
VERSION     DAOOBB010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19595_length_4901_cov_4.892076"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(274..2169)
                     /gene="speA"
                     /locus_tag="PLT31_07140"
     CDS             complement(274..2169)
                     /gene="speA"
                     /locus_tag="PLT31_07140"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HPW94947.1"
                     /translation="MRKWRIDDSRDLYNIRGWGINYFDINEKGHATVSPLKSKGPSID
                     LYELLQELAIRDVSTPMLLRFPDILDNRIEKINECFSKSIKEYNFNGTYYNVFPIKVN
                     QQRAVLEEVVRHGKKFNIGLEAGSKPELHAVLANMDNPEALIICNGYKDEDFIELALL
                     AQKMGKNIFIVVEKMNELHLVVELSRRIGVRPNIGIRIKLASNGSGKWEESGGYHSKF
                     GLNSSDLLEALDYIREEQMCDCFKLIHFHLGSQITNIRKIKSGLREVSQFYIQLKQLG
                     MNLEYVDIGGGLGVDYDGTRSSNASSVNYSVQEYANDVIYSIADACDKCEIAHPNVIA
                     ESGRALAAHHSILIFNILETAGPAFFDDEIHEIPEDAPDIVKDLYSIYKGLTPKNLLE
                     NWHDAIQLNDDALSGFKLGSVDLQTRAMTERLFWSIAQAVDRLSKDLRHPPYELSSLP
                     RLLAEKYFGNFSLFQSLPDSWAIDQIFPIMPIQRLNEEPIVEATLQDVTCDSDGKIDM
                     FVRGGEVSRTLPLHPLKKDEPYYMAVYLVGAYQEILGDLHNLFGDTNAVHIVCDEDTY
                     KIEKIIDGESVEDVLDYVNFSDKALVRTMENWVAGSVKEGKISLQEGKEFLNIYRSGL
                     YGYTYLE"
     gene            complement(2387..3652)
                     /locus_tag="PLT31_07145"
     CDS             complement(2387..3652)
                     /locus_tag="PLT31_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94948.1"
                     /translation="MALPQAEEVFWWWDDGLISYEEAEEFLLLLDSQDEEGFCSLLES
                     TLNQKCELEKQKKTKRKNQYAYINWKAEIDSLAQIQKQRLQIKSFFNPFVFEARPDSI
                     FTLTYERKKNKAILGHLTYSLLNTGIPLEPMNGYFGHLAIQNHSLSFLFTSNVDFGFQ
                     THFTKKPHQLHLHIYSFQKNFFFLSRYQNKSMDISLWVDLLKKLPLARIRLKTPPQKD
                     ELWHWQGELYIHTKDSLSSPLVLPKSVARSTIWSIQNQKFRFSFLELLFSEKINIPID
                     SNSTLASTGVSLKAFHSKGFFESSWACREMQQKCYKPQFRLHASIKPKKEIELFSKIK
                     LQGNSFSQLNHRPQLLFGFSILPEKNISFKNELIYPEKTSKKPFSWRQSTQIKTSPFW
                     SFLCHFEIQIQKKLNMHLYRTGLSFTLNY"
     gene            3838..4563
                     /gene="rsmG"
                     /locus_tag="PLT31_07150"
     CDS             3838..4563
                     /gene="rsmG"
                     /locus_tag="PLT31_07150"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HPW94949.1"
                     /translation="MSFNRKNKVRTDFPLFNGKRVSPSMEGLNKLMHFHGVELQKETL
                     EKLWAFHQMLREHNEDQDLTRLNAFETIVERHYADCTLINAFVPKWPSRMIDVGSGAG
                     FPGIPLKLVNPEIRLTLCEPRPNRIDFLNKVIQELGLKGIDVFGHKVTSRSMTIAIDG
                     VISRAFELMEKTLPRLENSLKVGGRVYFMKGPAVQEELETLRPEEYGYKLVGKHFYTI
                     PQSTQDRALIILERTEEKTSNAR"
BASE COUNT         1539 a          822 c          989 g         1551 t
ORIGIN      
        1 aagacattta cagagatttt ttagtgtcgt ttttatgtga cctaaaacaa gttgatacaa
       61 aaagactcag ttttctgaac ctattatcgt taatgctttt tgaaaaatag gcatttcttt
      121 tatgaagccc tattattgct taaagggtgt tatttgatgg taaacccact aggaaaaatc
      181 aaggtggttt ttattgaaaa aaatatgcct ataaaacaac gagtaaaaaa agatatgctt
      241 gatcaatgag acgaacatat ctgactttta ttcttactct aaataggtat agccgtacaa
      301 accagaacga taaatattca gaaattcctt accttcttgc aaggaaattt tgccttcttt
      361 aacagatcca gcaacccagt tttccatagt acgaacaaga gctttatcac taaaattaac
      421 gtagtctaat acatcttcaa cagactcgcc atcaataatc ttttcgattt tataggtgtc
      481 ttcatcgcag acaatatgga ctgcattggt atcaccaaaa agattatgta agtcgcctaa
      541 aatttcttga taagcaccta caagataaac ggccatgtaa taaggttcgt ctttttttaa
      601 tggatgtaaa ggcaatgttc ttgagacttc tccgccgcgc acaaacatgt caatttttcc
      661 atcggaatca caagtgacat cttgtaatgt ggcttctaca ataggctctt catttaagcg
      721 ttggataggc atgataggga agatttgatc aatcgcccag ctatcaggaa ggctttggaa
      781 taaagagaag ttgccaaagt atttttcagc taaaaggcga ggaagactgc tgagttcata
      841 aggagggtgt ctcaaatctt ttgataaacg atctaccgct tgtgcaatgc tccagaaaag
      901 tctttccgtc atcgcgcgag tttgtaaatc gacagagccc aatttaaaac cacttaaggc
      961 atcgtcattt aattgaatgg catcatgcca gttttctaaa agattctttg gggtaaggcc
     1021 tttgtatatc gagtaaaggt cttttacaat atcaggagcg tcttctggaa tttcatgaat
     1081 ttcatcatcg aaaaaagcag gccctgcggt ttctagaatg ttgaaaatca aaatagaatg
     1141 atgtgctgct agggctcgcc ctgattctgc aatcacgttt ggatgagcta tttcacattt
     1201 atcgcaggca tctgcaatag agtaaattac gtcattagcg tattcttgta ctgagtagtt
     1261 cacagaactt gcattactgc ttcttgttcc atcataatct acgccaagcc cgccaccaat
     1321 atcgacgtac tcgagattca ttccaagttg ttttaattga atataaaatt gagatacttc
     1381 acgaaggccc gatttgattt ttcggatatt ggtgatttgg cttcctagat ggaagtgaat
     1441 gagtttgaaa caatcgcaca tttgttcttc gcgaatgtag tcgagggctt ccaaaaggtc
     1501 tgaactattt aaaccaaact tgctgtgata gccgccagat tcttcccatt taccagaacc
     1561 gttgctagcg agtttaatac gaataccaat attaggccta actccaatac gtctagaaag
     1621 ctcaaccact aaatgaagtt cattcatttt ttccacaaca atgaaaatat tctttcccat
     1681 tttttgtgcg agaagagcga gttcaataaa gtcttcgtct ttatagccgt tgcaaataat
     1741 taaggcttca gggttatcca tgttggcaag aacggcgtga agttctggct tggaaccagc
     1801 ttctaaacca atattaaatt tctttccgtg acgaacgact tcttctaaaa cggcgcgttg
     1861 ttggttgacc ttgataggaa atacgttata gtatgttcca ttaaaattgt attctttgat
     1921 ggatttagaa aagcattcat taattttttc aattcggtta tctaagatat cagggaagcg
     1981 taatagcatt ggggtggata catcacgaat ggcgagttcc tggagtaatt cataaagatc
     2041 tatcgaaggg cctttcgatt ttaatggact gactgtagca tgcccttttt cgttaatatc
     2101 aaaataatta atcccccaac ctctaatgtt ataaaggtct ctggaatcat cgatacgcca
     2161 ttttctcatg atttaccgta ttggaaaagt gaaacgagat aaacgataga aaaatagaat
     2221 ggtattatga ataaggaaaa gaaataaaaa taaaaaagac gcctctaagt aaaggagggt
     2281 aacttagagg cgtcagggag tgtttgggta tgaataaatt acttaaacaa aaaagagaaa
     2341 acaaatcgtt ttcggatctt cctgtatacc taagtataca acgctattaa taatttaagg
     2401 tgaaagaaag ccctgttcta tagagatgca tattgagttt tttttgtatt tggatctcaa
     2461 aatggcataa aaaagaccaa aaagggcttg tttttatttg ggtgctttgc ctccaggaaa
     2521 agggcttttt tgacgttttt tctggatata ttagctcatt tttaaaagaa atattctttt
     2581 cggggaggat agaaaagcca aataatagtt gtgggcgatg gtttaattga gaaaaggaat
     2641 tcccttggag cttgatttta gaaaacagtt ctatttcttt ttttggtttt atggatgcat
     2701 gaaggcgaaa ttggggcttg taacattttt gttgcatttc gcggcaagcc caactggatt
     2761 cgaaaaatcc tttagaatgg aacgctttta aggagacgcc tgtgcttgct aaagtagagt
     2821 tagagtctat cgggatgttg attttctctg aaaaaagtag ttctagaaat gaaaacctga
     2881 atttttgatt ttgaatcgac cagattgtac ttcgggcaac gcttttcggg agtacgagtg
     2941 ggctagaaag agagtctttt gtgtgaatat ataattcccc ttgccagtgc caaagctcat
     3001 ctttttgagg aggcgttttt agtcggattc gagcaagcgg taactttttc aatagatcaa
     3061 cccataagga aatatccatg cttttatttt ggtatcgaga gaggaaaaaa aagttttttt
     3121 gaaaagaata aatatgcaaa tgaagctgat gcggcttttt tgtgaaatga gtttgaaatc
     3181 caaagtctac gtttgaagta aaaaggaagg agagtgaatg attctgaatg gcaaggtggc
     3241 cgaagtaccc gttcatgggc tcaagcggaa ttccagtatt tagtaggctg tacgttaaat
     3301 ggcctaagat ggctttattc ttttttcgct catatgttaa tgtgaaaata gaatctgggc
     3361 gtgcttcaaa aacaaaggga ttaaaaaaag actttatttg aaggcgctgc ttctgtattt
     3421 gagctaaaga gtcaatttct gctttccaat taatgtaggc gtattggttt tttcttttcg
     3481 ttttcttttg tttttctaac tcgcattttt gatttaaagt ggattccaga agagaacaaa
     3541 agccttcttc atcttgacta tccagtaaga gcaaaaattc ttctgcttct tcgtaagaaa
     3601 tgaggccgtc atcccaccac cagaagactt cttctgcttg agggagggcc atagagatga
     3661 tatttagcag taaaagatgc cagagtgttt ttttcataga gatttgtttg tttcactagg
     3721 taatgaaaca atcgttcctg ttttttttca gtgaataggt attgcacctt tctttaaacg
     3781 attaaaacaa agagtttttt taactggcgt agaaaaagtt tttttacatt tataaacatg
     3841 tcttttaata gaaaaaataa agtccgaaca gattttcctt tattcaatgg aaaacgagtc
     3901 tctccttcga tggaaggctt aaataagtta atgcattttc atggtgttga actccaaaag
     3961 gaaacacttg aaaaactttg ggcttttcat caaatgcttc gagaacataa tgaagatcaa
     4021 gatctcactc gattaaatgc gtttgaaacg attgtggaac gccattatgc agactgtaca
     4081 ttgatcaatg cttttgttcc caaatggcct tctcgaatga ttgatgtggg gagtggtgct
     4141 ggatttcctg gtattcctct gaaattagtg aatccagaaa ttcgccttac tttgtgtgaa
     4201 ccaaggccta atcgaataga ttttttgaat aaggtgattc aagaattggg cttaaaaggg
     4261 attgatgtct ttggccataa agtcactagt cgtagcatga cgattgcaat tgatggagtg
     4321 attagccgtg cttttgaatt aatggaaaaa acacttccac gtttggaaaa ttctttgaaa
     4381 gtgggtggac gagtgtattt tatgaagggc ccagcggttc aagaagaatt agaaacgtta
     4441 cgccctgaag aatatggtta taaactggta gggaagcatt tttacaccat tccacaaagc
     4501 actcaagatc gcgccttgat aatcttagaa cgcaccgaag aaaaaacctc taacgcccgt
     4561 tgagtttttt catgccgaga caagcgtatt tttgtaccac aagggtatca ctctcacagc
     4621 tatgcatggt tccgactaaa gcactaaaag tgctaagtct cacgtaaaat gctaaagcat
     4681 ataagtgttc gtgttcctgt aaagcaaata gcgcagattc aaatgaaaaa ataactcagt
     4741 taaattaaca aacactatta aaatagcaga cgggaatggt tgttattgat gagctcgttt
     4801 gaaatgcaat tgcacgaaaa tgagaagccc ttctaaacta acacagtttt tttcatgaag
     4861 aaccgaacgt tatttgattg gataatcaaa tagtgaaggt t
//
LOCUS       DAOOBB010000150         4885 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_19671_length_4885_cov_5.739130, whole genome shotgun sequence.
ACCESSION   DAOOBB010000150 DAOOBB010000000
VERSION     DAOOBB010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 4885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4885
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19671_length_4885_cov_5.739130"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..749)
                     /locus_tag="PLT31_07155"
     CDS             complement(165..749)
                     /locus_tag="PLT31_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94950.1"
                     /translation="MQHISFVKRAFLCFLLVSVMSFAAQKTAGGYYRDAAFQYIENRL
                     PTAEITCKEGLSYYPNDMKLQMLLDRIQEAKEEQKNKNKEQNKDQQDQNQDQEQNKDQ
                     QDQNQDQEQNKDQQDQNQDQEQNKDQQDQNQDQEQNQDQQDQNQDQNAPQEMPVGQMS
                     PEDASQLLKDFNEQNGEQKPWKPVRGQARPLKDW"
     gene            complement(749..1480)
                     /locus_tag="PLT31_07160"
     CDS             complement(749..1480)
                     /locus_tag="PLT31_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPW94951.1"
                     /translation="MNRLLFLLIFLVAIVEARPINEGNKLFAAGDYEGALAKYQKARE
                     SEPASPLLFYNIGTCYYQMGKWDEAKAELETALRMPDSSLASKAAYNLANTLFRKGES
                     QSEPSERIASWRESIAYLKKAIDLNPDFEKAKRNVEIVQRKLKEELDKQKEQKKEDQN
                     QDQKQPELSERAKEILARALQLSKEEQYAQAKTMLEQLLAEDETAAQLAPYIQRIDDI
                     IEIKAGRKPKAKIDTENVDNDLEVI"
     gene            complement(1802..2413)
                     /locus_tag="PLT31_07165"
     CDS             complement(1802..2413)
                     /locus_tag="PLT31_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94952.1"
                     /translation="MSFLDSSFELYHEIFGSVLDRIDLNPYLMRNSKGYADSLSRASL
                     MMGNRGFFLIKGELGVGEIERASQYVSQALHFMSDWLFPMVKNQLKSLLTMDYNDIEA
                     IERQKNYQKANQEIDIIEQVFNKTPFTECIQEDPRHLFIWVLLHKYPNLFDGYKKHKV
                     IPHSWSYAACAVLKISQDIYDFVCLGLFFEFHGIFWSNLYKYP"
     gene            2486..2845
                     /locus_tag="PLT31_07170"
     CDS             2486..2845
                     /locus_tag="PLT31_07170"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPW94953.1"
                     /translation="MKKDTKDKSLQAKTFQIFLRLLRQEKGLTQEALSISSGISRQHI
                     SEIEAKGVVPTILTQEAFAKAFNLSLSQFLSLFAEEYEKQKLCRKKGVSKDEYLQVAE
                     RSKKKQYRTNKDENSIK"
     gene            complement(2903..3766)
                     /locus_tag="PLT31_07175"
     CDS             complement(2903..3766)
                     /locus_tag="PLT31_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPW94954.1"
                     /translation="MKATLLLTAIAFLFLALSRPQWGRSLDHVERRGLDLVLLQDISL
                     SMLAEDVKPNRLVRSRHEISAFLESLTGDRVGLVAFSGEAKVMVPLTLDYGSVQMILR
                     DLDPGWLLPGTDLEKAIRKGIQMFQNSGGAGKYSVMILMSDGEELQNEAVKAAKEAAE
                     LGIRIYTIGIGSRDGVPIPLTTKSGEVSYKKDSHGNIVTTRLEEQTLQEVANVTGGLY
                     FYANPSEFQLQKVLTEIAMLEKKEHDSQSLETYQDRYQLFLGLSMFFFFVECLISERG
                     RRRRVTQGRFD"
     gene            complement(3928..>4885)
                     /locus_tag="PLT31_07180"
     CDS             complement(3928..>4885)
                     /locus_tag="PLT31_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPW94955.1"
                     /translation="KKASSTYRVKFRHVVPALRFLALICFIIALARPQKGVEVEYTST
                     DGVDIMLTLDVSGSMAILDMLTNSERMKLGVLNAERILKSGQFWNYSRLGYAKTVIAD
                     FIQKRTTDRIGLSVFAGNSFTQCPLTSDYGTLLEILSVVNDSTASGNGTAIGDGIMTS
                     LARLEKSEAKSRVMILLTDGRDNASLVPPLQAAEVAKALGVKVYTVGVGKRKGTVLAF
                     QQNPWTGEISWGEREIQPEEGIDEEMLKNVAAKTGGRFYRAENKEELKNIYDEIDQLE
                     KTEIQTVAYSRYSEKFYPWLLAGALLILLELLLANTRFVRIP"
BASE COUNT         1534 a         1010 c          781 g         1560 t
ORIGIN      
        1 cgatacaaat agaacatcga agccctgaac aacgacggat tacgcagttt attaacctaa
       61 ttcgactaga aacaagtaag tcgaggcgtg aaggctgaag ctgtacaaca agtacagcga
      121 acgcctgaac aacgacagat tacgaagttt atagtcgaat taaactacca gtcttttaat
      181 ggacgggcct gcccccgaac cggtttccaa ggcttttgct ctccattttg ctcattaaaa
      241 tctttgagta actgagatgc atcttctgga ctcatctgac ccacaggcat ttcttgagga
      301 gcgttttggt cttgattctg atcctgctgg tcttggtttt gttcctgatc ttgattctga
      361 tcctgctggt ctttgttctg ttcttgatct tgattctgat cctgctgatc tttgttttgt
      421 tcttgatctt ggttttgatc ctgctggtct ttattctgtt cctgatcttg attctgatcc
      481 tgctgatctt tattttgttc tttattctta ttcttttgct cttctttcgc ttcttgaata
      541 cgatctaaaa gcatttgtaa tttcatgtca tttggataat acgaaagacc ttctttacaa
      601 gtaatttctg cggtagggag tctattttca atatattgaa aggcagcatc acgataataa
      661 ccacctgctg ttttttgagc cgcaaaagac ataacagaaa cgagtaaaaa acaaaggaaa
      721 gctcttttaa caaaggatat atgttgcatt agataacctc caaatcattg tccacatttt
      781 cggtatctat ttttgccttg ggcttacgac ctgctttaat ttcaatgata tcgtctatcc
      841 gttgaatata gggagcgagt tgagccgctg tctcatcttc agccaaaagc tgttctaaca
      901 ttgtttttgc ctgagcatat tgttcttctt tacttaattg tagtgctctt gctaatatct
      961 cttttgctcg ctcagaaagc tctggttgct tttgatcctg attttgatct tctttttttt
     1021 gttctttttg tttatctaat tcttctttca atttacgctg cacgatctct acatttcgtt
     1081 tcgctttttc aaaatctgga ttcaaatcaa tcgccttctt taaataagca attgattctc
     1141 gccaagaagc aatcctttct gagggctcac tttgactttc gccctttcta aataatgtat
     1201 tagccaaatt gtaagcagcc tttgaagcta aagaagaatc tggcatacga agagcggttt
     1261 ctaattcagc cttggcttcg tcccatttcc ccatctgata ataacaagtt ccgatattat
     1321 aaaaaagcaa tggacttgca ggctctgatt ctcgagcctt ttggtatttt gctaaagcgc
     1381 cttcataatc ccctgctgcg aataacttat tcccttcatt tatagggcgt gcttcgacaa
     1441 tagccactaa aaaaatcaat aaaaacaata atcgattcac aagctaaact ccgtttttta
     1501 cccaagatat ttcatctgtt tggaatatac aaaaagttag aaaaatgaaa acagcttaaa
     1561 taatcaaaaa aatgtcaatt tttcgctaaa aatgtgtcat tcaaggcgtc tatatcgatc
     1621 tattcttaag ccaacaaaag ctttttttga aaaagaaaat atgaaagaaa acaatcaacg
     1681 gcctacattt acaagcctca gattgtcacc aacactgaat atcggaaata cgtaattcta
     1741 caggaaatgt atgatgacga gtgaaataaa atgctgcgca gagacagatt aattgatttt
     1801 ttcaaggata tttgtacaaa ttgctccaga aaatgccatg aaattcaaaa aataacccta
     1861 aacagacaaa atcataaata tcctgactaa tttttagaac agcacaagcc gcataactcc
     1921 aactatgcgg aatcactttg tgttttttat agccatcaaa caaattggga tatttatgta
     1981 ataaaaccca aataaataag tgtcttggat cctcttgaat acactcggta aatggcgttt
     2041 tgttaaaaac ttgttcaata atatctattt cttgattagc tttttgataa tttttttggc
     2101 gttctatggc ttcaatatca ttataatcca tcgtcaatag tgattttagc tgattcttta
     2161 ccataggaaa aagccaatcc gacataaaat ggagtgcctg agacacatat tgagaggccc
     2221 gttctatttc tcccactcca agttcaccct taattagaaa aaagccccta tttcccatca
     2281 ttaaagaggc ccttgaaaga gaatcagcat atcctttact atttcgcatt aaatagggat
     2341 ttaaatctat tcgatctaat actgatccaa atatttcatg atataattca aaagaagaat
     2401 caagaaaaga cataaaacac ctgttttttt gacggctcga gccgtcacta tttttcttta
     2461 tttttcataa catttatacg aaaacatgaa aaaagataca aaagataaaa gccttcaggc
     2521 aaagactttc caaatttttt tacgcctgtt acgtcaagaa aaaggattaa cacaggaagc
     2581 tctgtctatt tctagtggaa tatctagaca gcatatatcc gagatagaag ctaaaggcgt
     2641 tgttcccact attctgactc aagaagcatt tgcgaaagca ttcaatttaa gtctttcgca
     2701 gtttctctct ctttttgccg aagaatatga gaaacaaaag ttatgtagaa aaaaaggtgt
     2761 atccaaagac gaatacttac aagtcgctga acgtagtaaa aagaaacaat accgcacaaa
     2821 taaggatgaa aatagcatta aatagattat ttttttctct tcatcaaaaa gaggaaaaaa
     2881 caactgtttt atttaaaact atttaatcaa aacgtccttg agtaactcgg cgcctgcgtc
     2941 ctcgttctga aatcaaacac tctacaaaga aaaagaacat cgataagccc aagaataatt
     3001 gataccgatc ttgatatgtt tctaatgatt gactatcatg ctctttcttt tcaagcattg
     3061 ctatttcagt taatactttt tgtagttgaa attcacttgg attagcatag aaatagaggc
     3121 caccagttac atttgccact tcttgaagtg tttgttcttc taaacgagtc gtcacaatat
     3181 ttccatgaga atctttttta taagaaacct ctccagattt tgtggttaaa ggaataggca
     3241 ccccatctct agagccgatt ccaatagtat agattcgaat acccaattct gccgcttctt
     3301 tagcagcttt cactgcttca ttttgaagtt cttctccatc actcataagg atcatcaccg
     3361 aatactttcc agctcctcca gagttttgaa acatctgtat tcctttacga atagcttttt
     3421 caagatccgt tcctggcaac aaccaacctg gatctaaatc tcgaagaatc atttgaacag
     3481 agccataatc tagagttaaa ggaaccatca cctttgcttc tccagaaaaa gcaacaagtc
     3541 ccactcgatc tccagttaat gattctaaaa aggccgaaat ttcatgccta gaacgcacca
     3601 aacgatttgg cttcacatct tcagcaagca tcgaaagaga aatgtcttgt aacagaacta
     3661 aatccaatcc tcttcgttct acatgatcca aagagcgtcc ccattgaggt cttgataaag
     3721 caagaaacag aaacgctatt gcagtcaaaa gaagtgtagc cttcaccaat cttcgccaag
     3781 gagacaatga tgttgccact ttaggtaaca tcgagagaga cacaaaacga gtagctagtt
     3841 tttttctcct atgataagca taaaagaaaa gcagagcgaa tagaggaagt gaaaataaaa
     3901 gccataaaaa atggggttca gcaaatctca tgggatcctc acaaatcggg tattagctaa
     3961 taaaagttct aatagaatta acaaagcacc agccaaaagc caaggataaa atttttctga
     4021 atatcttgaa taggcaacag tttgaatttc tgttttttct aactgatcaa tttcatcata
     4081 tatatttttc agttcttctt tattttctgc tcgatagaag cggcctcctg tctttgcagc
     4141 cacatttttc aacatttctt cgtcaattcc ttcttcaggt tgtatttctc tttctcccca
     4201 agaaatttct ccagtccatg gattttgctg aaaagctaaa acagtccctt ttcttttccc
     4261 aacgcccact gtatacacct ttacgcccaa agctttagcg acttctgccg cttgtaaagg
     4321 cggcactaaa ctcgcattat cacgaccatc tgttaaaaga atcatcactc tcgattttgc
     4381 ttcagacttc tcaagtcttg ccagtgaagt cattatacca tctccaatag cagtcccatt
     4441 tccagaagcc gtcgagtcat tcaccacaga aagaatttct aatagcgtcc catagtcaga
     4501 agttaatggg cattgtgtga aagaattgcc ggcaaaaaca gataatccta tacgatctgt
     4561 tgttctcttt tgaataaagt ctgcaatcac tgttttagca taccctaaac gactgtaatt
     4621 ccaaaactgt cctgacttta atattctttc tgcattcaaa acccctaact tcattctttc
     4681 tgaattagtc aacatatcaa gaatggccat agagccagag acatctaagg tcagcataat
     4741 atcaacacca tcagtagatg tatattctac ttcaacaccc ttttgaggcc ttgcaagtgc
     4801 gataataaaa caaattagag ctaaaaagcg aagtgctgga acaacgtgtc taaattttac
     4861 tcgataagta gagctcgcct ttttt
//
LOCUS       DAOOBB010000151         4880 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_19691_length_4880_cov_5.103834, whole genome shotgun sequence.
ACCESSION   DAOOBB010000151 DAOOBB010000000
VERSION     DAOOBB010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 4880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4880
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19691_length_4880_cov_5.103834"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /gene="bioD"
                     /locus_tag="PLT31_07185"
     CDS             <1..420
                     /gene="bioD"
                     /locus_tag="PLT31_07185"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HPW94956.1"
                     /translation="SPHLAARKEGNPLELSKVVSDYQLLKSKHDFIFVEGSGGIVCPI
                     RYDNQKIFLEDIIKALGLEIILVSNALLGSINATVLSVEYARSKGIGIRGIILNQYQG
                     SEMEQDNYKMIQEITGVPILAAVPLNAKEIELNIEGF"
     gene            513..1774
                     /gene="bioA"
                     /locus_tag="PLT31_07190"
                     /pseudo
     CDS             513..1774
                     /gene="bioA"
                     /locus_tag="PLT31_07190"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
     gene            complement(2082..2843)
                     /gene="bioC"
                     /locus_tag="PLT31_07195"
     CDS             complement(2082..2843)
                     /gene="bioC"
                     /locus_tag="PLT31_07195"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: protein motif:HMM:TIGR02072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HPW94957.1"
                     /translation="MPIHIEKSLIKERFCKSIKTYSEYATVQATIAKRLAQKLKEHGQ
                     EFNTVLELGAGTGFLTRELEEQFKVNSLTCNDLVGDYKPLLESLTEKSSFSFIEGDLE
                     QTELFQGSYDLIASASTLQWILELDSLFEKLKAKMNPNAIFAFSTFGKKNMVEIRSIL
                     QSGLYYPSLEIVLEIVQKHFSLLHYDEEIHSVSFNDPVDVLKHIKRTGVNALFQTHWT
                     KGDLHRFSSQYKESFSSDNQVTLTYHPMYIIAQKK"
     gene            complement(2816..3499)
                     /locus_tag="PLT31_07200"
     CDS             complement(2816..3499)
                     /locus_tag="PLT31_07200"
                     /inference="COORDINATES: protein motif:HMM:NF016209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HPW94958.1"
                     /translation="MKHYYLNKKNQSKLILFFCGWGNDYHPFLNLLCKDYDVLMLYDY
                     RDLKTEIDFQELLASYKEVHLISWSLGVFVANHLFQDVPFSSTSAINGTLQAIDDRLG
                     IPVAIFDGTIQLFNERNRDKFFKRMCLHPEAITFFEKNKPERELDEQLEELKILRDLI
                     LNHPPKKNIYNRAIIGTEDAIFPPQNMIHYWNGLVTEENLIQLEAPHFLFHLYRQWSD
                     LCPSILKNH"
     gene            complement(3496..4659)
                     /locus_tag="PLT31_07205"
     CDS             complement(3496..4659)
                     /locus_tag="PLT31_07205"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HPW94959.1"
                     /translation="MKDSRYSKILQSLHESSQLRFLREMSQKHGSRLEFEGKKYINLS
                     SNDYLGLAFDSSLLTEFYKSLSKETVVNSYGLGASSSRLHTGNSPLYTQVEKKLASLY
                     SKEAALIFNSGYHANIGILPALSSKKDLILSDKLNHASLIDGFKLGDATLVRYRHFDY
                     EQLEEILTLRCQEFDHVFIVTESIFSMDGDIANLKRLVEIKNKFNAFLYVDEAHAFGC
                     RGEHGLGICEEQGVTSEVDFIVGTFGKAIGSQGAYIVCSQITRDYLINTMRSLIFTTG
                     LPPVTLSWLLFILERLGEFKEKRAHLLSLSKLIRGYLQSNGKTTVSESHIIPIILGEN
                     KEALLAAQKWQENGFLVFPIRPPTVPMGTARLRLSLTADMDESVLKPLQSLIP"
     gene            complement(4673..>4880)
                     /locus_tag="PLT31_07210"
     CDS             complement(4673..>4880)
                     /locus_tag="PLT31_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94960.1"
                     /translation="LQKYVDLHPDAKIDFDYQDRIFPEISGHYQSNQGLITFTPSSSF
                     SKEVLNPKQVLSLYPLNGGLRLKV"
BASE COUNT         1549 a          845 c          961 g         1525 t
ORIGIN      
        1 tctccgcatt tagcggcaag aaaagaaggg aatccgctag aattatcaaa agtcgtttct
       61 gattaccaat tattaaaatc taaacatgat tttatttttg tggaagggag cgggggaatc
      121 gtttgcccta ttcgttatga taaccaaaag atttttttag aagatattat caaggctctt
      181 ggtttagaaa ttattctagt ttctaatgcc ttattaggct cgattaatgc caccgtactt
      241 tccgtagaat acgctcgctc taaggggatt ggcattcgag ggattatctt aaatcaatac
      301 caaggctctg aaatggagca agacaattat aagatgattc aagaaataac gggagtccct
      361 attctcgcag cggttccatt aaatgcaaaa gaaatagagt taaatataga ggggttttaa
      421 tgaattctaa agaatcttta attgctaaag accttcaata tatttggcat ccttgttcgc
      481 aaatgaaaga ttatgaagaa ttgcccccta ttatgattga ccatgcaaag ggctcttatc
      541 tatatgacat tcatggcaag cgatatatcg atatcattag ttcctggtgg tgtaatcttt
      601 taggccattg taatcctcat attagtgatg caatcaagaa acaattagac aagctagaac
      661 atgttatctt tgcgaatttt acgcatgaag gggcggtgct cttgtgtgaa cgattgatga
      721 agcatttacc taaggggctc actaaattta atttttcaga taatggatct gcttctattg
      781 agtgtgctct taaaatgagc tttcaatacc aataccaaac agggcatcca gaaaaaaaca
      841 tttcatgacg ctttcggatg ggtatcatgg tgaaaccgtg ggagctcttt ccgttggtgg
      901 cttagactta tatgccgaaa tttataagcc catgctgatg gatgtgattc atgtagaagc
      961 gcctaattgt taccgttgcc catacaaaaa acatcgagat tcttgctcta tagaatgctt
     1021 tgaaaaagta aaaaaagcat ttgcattata cgcaaaagaa acatgtgcct ttatttgtga
     1081 gcctctttta caaggttcag ctgggatgcg catgtaccca ccacaatacc ttcgcgcgct
     1141 acgagatctt tgtacagaat acgatgtgat catgatagct gatgaaattg cgacgggatt
     1201 tggacgcaca gggacgtttt ttgcctgtaa tcaagcttct gttgctccag atattatgtg
     1261 tctttctaag ggccttactg gcggttatat gccaatggcc ttaacgatta cgacgccaaa
     1321 aatttatgat gctttttatg ccgattatct tgaagggaag gcttttatgc atagccatac
     1381 ttatagcgga aatccgctcg gttgtgcggc ggcgcttgcg gtactcgatg ttttagaaaa
     1441 tgaatctatt atagagaagg ctaaagaaaa agcggcttat ttacacgcgc ttttagtgga
     1501 tacgcttgga acgcatcctc atgttggaga aattcgtcac ttagggatga ttaacgccat
     1561 tgaattagtg gccgatgttc aagtgaagac tccttttgat tctaaaaagc gtacggggta
     1621 tcagatttat aaaaaagcgc tttctaaagg cttattatta aggcctttag gcgatgttct
     1681 ttattttaat ccgccgctca cgatttcttt agaagaaatt caagaggccg tttcgatttg
     1741 taaagaagca ttacttgaaa tactaccaga ataaaaaacg ggctcgtatt taaataggtt
     1801 tgttttgaac ataaggtggc ttctattatt tgagcctacg cgtttgctga tacaaaacga
     1861 cctgcggaat attatcaatt ctgctttctt aaagaaatta taaaaagtca taaaataacg
     1921 acgtcaaaaa aatatgaatt atttgtttca agtgaaacga taaataaaga cattaaagac
     1981 ttttacggtt tattttaagc gaatcaataa aaaatgatgt tcaaaaaatc cgttatgttt
     2041 tgttttgaac ataacgggaa aaatggtatc taaaaaacgg attacttttt ttgagcaatg
     2101 atatacatcg gatggtacgt taaagtcact tgattgtctg atgaaaacga ttctttatat
     2161 tggcttgaaa agcggtgtaa atcgcctttt gtccagtgcg tttgaaacag cgcatttact
     2221 cctgttcttt tgatatgctt tagaacatcg actggatcat taaaagaaac agaatggatt
     2281 tcttcgtcat aatgtaaaag tgaaaaatgc ttttgcacta tttctaaaac gatttctaaa
     2341 gaagggtagt aaagaccact ttgtaaaatg gatcgaatct ccaccatatt ttttttccca
     2401 aaggtggaaa aggcaaaaat ggcgtttgga ttcatctttg cttttaactt ttcaaaaaga
     2461 gaatctaatt cgagaatcca ttgtagtgta gaagcagagg caattaaatc ataagaccct
     2521 tgaaagagtt ctgtttgttc taaatcgcct tcgataaaag aaaaagaaga tttttctgtc
     2581 aaagattcaa gaaggggttt ataatcgccg acaagatcgt tacaagtgag agaattgact
     2641 ttgaattgtt cctctaattc acgcgtgaga aatccagtcc ctgcgccaag ttctaaaacg
     2701 gtattaaatt cttggccgtg ttcttttaat ttttgggcga gccttttggc aatggtcgct
     2761 tgaacagtcg cgtattcgct ataggttttt atgcttttgc aaaaacgttc tttaatcagt
     2821 gatttttcaa tatggatggg cacaaatcac tccactggcg gtataaatga aataaaaaat
     2881 gaggagcttc taattggatt agattttctt ctgtgacaag cccattccag taatggatca
     2941 tattttgagg aggaaatatc gcgtcttctg ttccaataat cgctctgttg taaatatttt
     3001 ttttaggcgg atgattaaga attaaatctc taagaatttt taattcttct aactgctcgt
     3061 ctaattcgcg ttcgggctta ttcttttcaa aaaaagtgat agcctctgga tgcaaacaca
     3121 tgcgcttaaa aaacttgtct cgattgcgtt cgttgaaaag ttgaatcgtg ccatcaaaaa
     3181 tagcgacagg aatcccgaga cgatcatcta tcgcttggag cgtcccgttg atcgcgcttg
     3241 ttgaagaaaa gggtacatct tgaaataaat ggtttgccac aaaaacgcca agcgaccaag
     3301 aaatcaaatg gacttcttta taagaggcaa gtaattcttg aaaatcgatt tctgttttca
     3361 aatctctata atcataaagc atcaagacgt catagtcttt gcataaaaga tttagaaaag
     3421 gatggtaatc gtttccccag ccgcaaaaaa agagaatcag ctttgattga ttttttttgt
     3481 ttagataata atgcttcatg gtatcaaaga ttgcagaggt tttaggaccg attcatccat
     3541 gtctgcggtt aaagagaggc gaaggcgtgc tgtacccata ggcaccgtag gaggccgaat
     3601 cgggaataca agaaaaccgt tttcttgcca tttttgagca gcaagtaatg cctctttatt
     3661 ttcccctaaa ataataggga taatatggct ttcacttacg gtcgttttac cattgctttg
     3721 caaatagcct cgaattaatt tagagaggga caaaagatga gcacgcttct ccttaaactc
     3781 tcctaatctt tctagaataa atagtaacca agataaggtc acaggaggga gccctgttgt
     3841 gaaaattaga gaacgcatgg tattgatcaa atagtctcga gtaatttgag aacacacaat
     3901 ataagcccct tgagagccaa ttgctttacc aaaagtacct acaataaaat ctacttctga
     3961 agtgacgcct tgctcttcac aaatcccaag cccatgttca cctctacatc caaaagcgtg
     4021 tgcctcatct acatacaaga acgcattaaa tttgttttta atttctacta agcgttttaa
     4081 gtttgctata tcaccatcca tactaaaaat ggattctgta acaataaaaa catgatcaaa
     4141 ttcttgacac cttaacgtga gaatttcttc tagttgttca taatcgaagt ggcgatagcg
     4201 aacaagagtg gcgtctccaa gcttaaaacc atcaattaaa ctagcgtggt tcaatttatc
     4261 agaaagaatt aagtcttttt tactagaaag agcggggaga attccaatat tggcatgata
     4321 gccactatta aaaatcaagg ctgcttcttt agaatataag ctagcgagtt tcttttcgac
     4381 ttgagtgtaa agaggagagt ttccagtgtg aagtctagaa gaagaagccc ctaacccgta
     4441 agaattaact acagtttctt ttgaaagaga tttataaaac tcggttaaaa gactcgaatc
     4501 aaaagcgagc cctaagtaat cgttagagga tagattaatg tactttttcc cttcgaattc
     4561 aagacgagat ccatgctttt gagacatttc tcgtaagaat cgtagttggc ttgattcatg
     4621 aagactttgt agaattttag aataacgcga atctttcatc tgataaactt ccctatactt
     4681 ttaatcttag gcctccattt agaggatata aggaaagtac ttgcttagga tttagaactt
     4741 cttttgaaaa agaggaggac ggcgtaaacg taattaaccc ttgatttgac tgataatgtc
     4801 cagaaatttc agggaaaata cggtcttgat aatcaaaatc gatttttgca tctgggtgta
     4861 aatcaacata tttctgtaaa
//
LOCUS       DAOOBB010000152         4823 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_19962_length_4823_cov_3.731124, whole genome shotgun sequence.
ACCESSION   DAOOBB010000152 DAOOBB010000000
VERSION     DAOOBB010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19962_length_4823_cov_3.731124"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..549
                     /locus_tag="PLT31_07215"
     CDS             181..549
                     /locus_tag="PLT31_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94961.1"
                     /translation="MALISFMLFILAIMVSFAILINGQFNRNHETQKKRLEHRLAMLE
                     KFISFYGRAQEYHSFDGFYELQLLFGLYGEDDEKKSMDYIISLIKSNSKNVELPKQLN
                     ELYILLTERLRAELESKRLA"
     gene            960..2699
                     /locus_tag="PLT31_07220"
     CDS             960..2699
                     /locus_tag="PLT31_07220"
                     /inference="COORDINATES: protein motif:HMM:NF012671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 11 protein"
                     /protein_id="HPW94962.1"
                     /translation="MSINKILAAIFGMMAILSSLSWGQTYTCSNGKFQQMGSAGGYDY
                     ELWSQDGAGSATMTLNPSDANGGTFSVEWSGTVNMLARSGKRWGAQSTVTVQNVGNIT
                     AEFEVEWSSTDNVKYVSVYGWGYYDQQDIPSGFSNEIEYYIIQDRGSYNPTQGGKKFG
                     SATIDGINYDFFTTDRNNQPSLSGTSTFKQYWSIPSNTSQHRTKGTISISKHFEAWAK
                     AGMKMGKLYEVASMKIESYTGQGGNARGSATVKKNLLRIGGSANEFALNTTVSPAAAG
                     TITKSPSAAYYAPNSTVQLTATANEGWKFVGWEGAATGSSNTASVSITDKDLSVTAKF
                     ELVGETTVNLFKDGDFPSSSVISTSNDASWFLGQGENWGSSAATTSVAGGTATVNVTT
                     IGTEPYQPQLVQYNLALDKGMNYKLTFKASADVARKIAVAFQQSVDPWGDYATKEFDI
                     TTTEQEYTFIFTMEEDSDPTSQFAFNLGQATGTVKISDVKLVYTNLVPDYISRQMVTV
                     NHLFSVNGRNLNVSPANGSVQVKIVDIHGKVKASFNTASAATFSLANIPAGVYYVNAK
                     GAGVNQLKSIVLR"
     gene            2797..3915
                     /locus_tag="PLT31_07225"
     CDS             2797..3915
                     /locus_tag="PLT31_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HPW94963.1"
                     /translation="MTDPRITQLAENLINNAICLKAGENVLIETTDTPEELSTELIKA
                     VEKAHGNAFVRNFNSRVRRQSIKSATEVQLKLEAELSLAEMKQMQAYIAIRGANNAME
                     NGDIDDEKMLMYRKLTKETLDYRVNKTKWCILRWPNPSMAQGAKMSTEAFEDFYFKAC
                     LADYPKMKSAAQNLIDLMNKTDKVRLVAKDTDITFSIKGIPAVPCCGNMNIPDGEVYT
                     APVQGSIEGVIHYNTPTLYEGKQFSGVRLVFKKGKIVEATCESGDNDQLQAIFNTDEG
                     ARYVGEFALGFNPFVNAPMCDILFDEKIAGSIHFTPGMCYEDAYNGNQSAIHWDLVLI
                     MRPEYGGGEIYFDDQLVRKDGLFVVDSLKCLNPDQLGV"
     gene            4491..>4823
                     /locus_tag="PLT31_07230"
     CDS             4491..>4823
                     /locus_tag="PLT31_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94964.1"
                     /translation="MKKRMAFGVACVLSVAISSAFTITPNPIVSSGALAYANGSSSTY
                     LTDGNLLNWKSVNAKDIAIQVTAGPSKLFIHWESFGDCAWATDFTTGCGHSGTAFKNF
                     KVMTSANST"
BASE COUNT         1477 a          880 c          977 g         1489 t
ORIGIN      
        1 cagggaaaaa aaagaaaaaa cacataaaaa ggctatttta tgtggctttt tttctaaaaa
       61 aaggggaaaa agtgagaaaa agcatagaaa tcatttctat gttcatatgg aacgaatgat
      121 cgtgattaaa gtatattctt gaaataaaaa aagataagtg atatggataa ggagtaaatc
      181 atggctttaa ttagttttat gctgttcata ctagctatta tggtttcttt tgccatactt
      241 attaatggac aatttaatcg aaatcatgag acccagaaaa aaagactaga acatcgttta
      301 gcgatgctag aaaagtttat ttctttttat ggaagagctc aagaatatca ttcctttgat
      361 ggtttttatg aattacaact cctttttggt ctatatggcg aagatgatga aaaaaagagt
      421 atggattata tcatttcatt aatcaagagt aattccaaaa atgtcgagct ccctaaacag
      481 ttgaatgaac tatatatcct tcttacagaa agattgcgag ctgagctcga atctaagcga
      541 ttagcttagc gttttaatca aaatttttag aggactgaaa aggtattatt ttttttacat
      601 cgtttaaaga gagaataaga aaaaatgtac tcattgtatt ccattcgaaa cgcctgtttt
      661 attaagaatc tcatttatag aaacgttact tattaaataa atgttctcaa aaaaggatgt
      721 tgtaaatagt tacaacagat ttgatctttt cactttcaac tttactccgt ttttgggttt
      781 aataaaataa atttagtatg tgtgtaatcg ttgtttctag tctggtgtac aaacgatttc
      841 ggataaagtg aattatcctt tttgttgtgg gtctgtggtt attccttcta taaaaaggct
      901 tgtgtgtggg tacagtttgt gtccatgacg aaaaaaaata aaactttgaa aggagttgta
      961 tgagtatcaa taaaatttta gcagccatct ttggaatgat ggcgattctg tcttcacttt
     1021 cttgggggca gacttatact tgtagtaatg gtaaatttca acaaatgggt agtgctggtg
     1081 gctacgatta tgagctctgg agtcaagacg gtgcagggtc tgctactatg acgctcaacc
     1141 caagcgatgc caacggtggt actttttccg ttgagtggag tggaacggtc aatatgctcg
     1201 ctcgttctgg taaacgctgg ggagcacagt ctaccgttac cgtacaaaat gtaggtaata
     1261 ttaccgccga atttgaagtg gagtggagtt ctaccgacaa tgtgaagtat gtcagtgtat
     1321 acggctgggg atattatgat cagcaagata taccgagcgg gtttagcaac gaaattgagt
     1381 attatattat tcaagatcgt ggaagttata acccaacgca aggcggaaag aagttcggtt
     1441 ctgcaaccat tgatggcatt aactatgatt tctttaccac cgaccgcaat aatcaacctt
     1501 cgctttcggg aaccagcact tttaaacaat attggtctat tccaagtaac acaagccaac
     1561 atcgtaccaa aggaaccatt tctatttcca aacactttga ggcatgggca aaagcgggca
     1621 tgaaaatggg caaattgtat gaggttgctt cgatgaaaat cgaatcctat actggacaag
     1681 gaggcaatgc tagaggttct gctactgtta aaaagaatct attgagaatc ggcggcagcg
     1741 caaacgaatt tgctctcaat acaaccgtat ctcctgcagc agctggtaca attactaaga
     1801 gcccaagtgc tgcctattat gctcctaact ctaccgtaca actcacagct acagctaatg
     1861 aaggctggaa gtttgttggt tgggaaggtg ctgccacagg ttcttccaat acggcttcgg
     1921 tatccatcac tgataaagat ctttccgtta ctgctaagtt tgaattagta ggtgaaacca
     1981 ccgtgaacct tttcaaagat ggtgacttcc caagcagcag cgtgatttct acaagtaatg
     2041 acgcttcatg gttccttggc caaggtgaaa attggggtag ttctgcagcc acaacatctg
     2101 ttgctggtgg cacggctaca gtgaacgtta ccactatcgg aaccgaacca taccagccac
     2161 aacttgttca atacaatttg gctcttgata aaggcatgaa ttataaattg acctttaaag
     2221 ctagtgccga tgtggctcgt aaaatcgcag tcgctttcca acaatccgta gacccatggg
     2281 gtgattacgc gacaaaagaa tttgatatta ccaccacgga acaagaatac acctttatct
     2341 ttaccatgga agaagattct gatccaactt cacaatttgc cttcaactta gggcaagcta
     2401 ctggtacagt caaaatcagt gatgttaaac ttgtttacac caatttggtt ccagactaca
     2461 tctctcgtca aatggtgacc gtcaatcact tgttctctgt caatggaaga aacctaaacg
     2521 tttctccagc aaatggtagt gtacaagtca agattgtaga cattcatggc aaggtgaaag
     2581 caagctttaa tacagcaagt gcagctacat tctcactagc taatatccct gcaggtgttt
     2641 attatgtaaa tgctaagggt gctggtgtaa accaactcaa gtctattgta ttaagataat
     2701 tttcttagaa ctccttaaaa aaagtctagc gtttttcgct aggctttttt ttttattttt
     2761 gtaaattaaa acatcgaatt taatcttggg aaaaaaatga ctgatccacg aattacacaa
     2821 cttgctgaaa acttaattaa caatgccatt tgcctcaaag caggcgaaaa cgttcttatt
     2881 gaaacgaccg atacccccga agaactctcc acagaactca ttaaggccgt agaaaaagct
     2941 catgggaacg cttttgttcg taattttaat agtcgagttc gtcgccagtc tataaagtct
     3001 gcaacggagg ttcaattaaa gctagaggct gaactctctt tagccgaaat gaaacaaatg
     3061 caagcctaca ttgccattcg tggggcaaac aacgccatgg aaaacggtga tattgatgat
     3121 gaaaaaatgc tcatgtacag aaagctcacc aaagaaacac ttgactaccg ggtgaataaa
     3181 actaaatggt gtatccttcg ttggcctaat ccttctatgg ctcaaggcgc taagatgagt
     3241 acagaagctt ttgaagactt ttattttaag gcctgcttag ccgattaccc taaaatgaaa
     3301 tcagcggctc aaaaccttat tgatttgatg aataaaacag ataaagtgcg tttagtcgct
     3361 aaagacaccg acatcacttt tagcattaaa gggattccag cagtaccttg ttgcggcaat
     3421 atgaacattc ccgatggtga agtttatacc gctccagtgc aggggagtat tgaaggcgtg
     3481 attcattata acacccctac attatacgaa ggtaaacagt ttagcggcgt tcgcttagta
     3541 tttaagaaag gtaaaattgt cgaagccacc tgcgaatcag gcgacaacga tcaactccaa
     3601 gcaattttta ataccgacga aggcgctcgt tacgttggcg aatttgcact cgggtttaat
     3661 ccttttgtaa acgctcccat gtgcgatatt ttattcgacg aaaaaattgc gggcagcatt
     3721 cactttactc caggcatgtg ctacgaagac gcttacaacg gcaaccaaag cgctatccat
     3781 tgggatttag ttttgataat gcgcccagaa tacggtggcg gtgaaatcta ttttgatgat
     3841 cagcttgttc gtaaagacgg tctttttgta gtagactcac tcaagtgcct aaacccagac
     3901 caactaggcg tttagctcgt ctgcactttg aaaacgtttc atttcttcac ataaagatat
     3961 catcgctgct tttaaacgga actcaagccc cgccacttcc tcctcattaa gccaaaggac
     4021 ggagctctgt tagcgttttt tcattaatgg tatttaaatg ataaggtcat gtattgaatt
     4081 tttttattaa aaaagtttta ttttataaca ttttaattta attttttttc aatcgggttt
     4141 ttgtaagggg ctctttatgt caaaaattgt tgctatatca gatcaaatag tttctattgg
     4201 tttggaatat cttaggctct ttcatcattg tagggccttt cattgcaatg tataaaatca
     4261 tcaacgcctt gaattttgta aatgatcatt acaacacctc acactaagtt ttgatcaaaa
     4321 ttctaaaaaa actagctcag tgattcgcta gttttttttg cattttcaat ttttgagagg
     4381 aattttttaa gtgtggcctt tggtgtccac acttgtggat ttttttttgt gctcaaaaga
     4441 ggggagaaac ttattttaga gtcgtgtttg tgcctaaaaa gggagttttt atgaaaaaac
     4501 ggatggcttt tggtgttgcc tgtgtattga gtgtagcgat tagttctgct tttactatta
     4561 ctcctaatcc tattgtaagt agtggtgctt tagcttatgc aaatggatct tcgtcgactt
     4621 atttaaccga cggcaactta ctgaattgga aatctgtaaa tgcaaaagat atcgctattc
     4681 aagtgaccgc agggccatca aaacttttca tccattggga atcttttggg gattgcgcgt
     4741 gggcgactga ttttaccaca ggttgtggcc atagtggcac tgcttttaag aattttaaag
     4801 tgatgacttc tgctaattct acg
//
LOCUS       DAOOBB010000153         4759 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_20306_length_4759_cov_4.179209, whole genome shotgun sequence.
ACCESSION   DAOOBB010000153 DAOOBB010000000
VERSION     DAOOBB010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20306_length_4759_cov_4.179209"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="PLT31_07235"
     CDS             complement(<1..624)
                     /locus_tag="PLT31_07235"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HPW94965.1"
                     /translation="MSALHIKHLFGLRGVSQSDIRTILDTAMDFRQVLERPVKKVPSL
                     RGVTVVNLFFENSTRTRTSFELAEKRLSADTINFTSSNSSVKKGETLLDTLRNIEAMK
                     IDLVVVRHKGTGVPQFLADNSQAIIVNAGDGAHEHPTQALLDMLTIEQHLGSLKNKRI
                     TIIGDIRHSRVARSNLWGLTTMGAQVTLCAPSTLTPRNREFFNGVHFE"
     gene            complement(621..1187)
                     /gene="pyrR"
                     /locus_tag="PLT31_07240"
     CDS             complement(621..1187)
                     /gene="pyrR"
                     /locus_tag="PLT31_07240"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HPW94966.1"
                     /translation="MKDNCKRIRLLLDAKSMEWALDEMAAKLAKMHPSPENLIVIGMA
                     TRGIPLAEKLASRLSEKYGQKIPLGSIDATFYRDDFHYRKRIQNTEMRIKAMPTSVED
                     KEIILVDDVLYTGRSVRAAMQSILDLGRPSAIRLCVLVDRGHRELPIAPDCVGLVVET
                     AVNQEVRVSIEPMDNENSVYLVEVEGEV"
     gene            1364..1912
                     /locus_tag="PLT31_07245"
     CDS             1364..1912
                     /locus_tag="PLT31_07245"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPW94967.1"
                     /translation="MFNQLDKAQSGETIAIIKTNHGEMRARLFPEIVGESAENFIKLA
                     QQGKYDNVPFHRIIKGFMIQGGDFTNRNGTGGHAAKGPGCNIGDCYDARLTHLRGALS
                     WAKTSLPNSIGSQFFIVHPEEGTHFLDHPANGGAAEGYSVFGQLFDGFDVLDAIASVK
                     TDRNDRPHEDVLIESVVIEKMA"
     gene            1973..2045
                     /locus_tag="PLT31_07250"
     tRNA            1973..2045
                     /locus_tag="PLT31_07250"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2006..2008,aa:Ala,seq:ggc)
     gene            2127..3911
                     /locus_tag="PLT31_07255"
     CDS             2127..3911
                     /locus_tag="PLT31_07255"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPW94968.1"
                     /translation="MFRLFFLVLFVGASFAADYSPFNKEAYLTSSFAENRGSRYHAGI
                     DFSTEMEEGWPIFAPEDGEIQDIKVSPFGYGKVIYFKGESGKLWVFAHLSSFNESIEN
                     LVARRQSSERKNDVSIILPDLDRIKKGDTLAYSGSTGIGAPHLHIETRITKNKIVSPC
                     QNGVQCLDTIAPFILGAAAWRNHEVIMTNEQSLQEGCLAVPNEDKPLKLAFKIVDYSR
                     TPLENPMSIRRIELKVEKRSLYKRVKDVLSYSTMLKIRDELLWAEEADTAGDWHLMLG
                     ALPTERTIRLEVEDFSGNISFRDFTFQPSCSTNTKASYFKQQDSTLFTFLSRSFLNLS
                     ECSKTQFELKDAQNKTLATNLCHIYPNVETPISLFFEKYPSAAKISVKRFNGKGNREI
                     LLFPLSGELQSKIEIQKPYLLTSEIKGLKNKYINQVIAIQETKKDSITSLEFHPKGLQ
                     FFKNWHVCFDTASVKDPIYWLGETSREWFVFSKQTKEKNKRCIQTNELRDIASIRDTI
                     PPAPGKPYYKDEKLRIPVIEQYAGILNGNAFKVNSTEEPWITAEYDSDPKELTIKKDL
                     LPPAGDSISVFMQDVLGNSNTYSIIIPE"
     gene            complement(3914..4624)
                     /locus_tag="PLT31_07260"
     CDS             complement(3914..4624)
                     /locus_tag="PLT31_07260"
                     /inference="COORDINATES: protein motif:HMM:NF021601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HPW94969.1"
                     /translation="MWNKKKKCLLSFHDISIENFDTISLLLEEIRLLAGCPFALLIIP
                     DTKNASVERIKAFKKQLKEWREDGYEILLHGCSHLSTESHQRSYLGQIAQILTHREAE
                     FAGLNQKDSLILLQKALDFWFFLDLDSPEVFIAPAWYDNPFLASQVLMQEMRYEGRFT
                     IRTKKKIYFSVAISFAGLVKPLVPIAFFFGHLLLRIPFIIPRIALHPVDFPTHREQIH
                     NLIRSALNQSHWLRYKDL"
BASE COUNT         1485 a          933 c          850 g         1491 t
ORIGIN      
        1 ttcaaagtga actccattaa aaaactccct attacgagga gtcaatgtgc ttggagcaca
       61 aagtgtcact tgagcaccca tagtggttaa gccccataga ttagaacgag ctactcgact
      121 atgacggata tctccaataa tggtaatgcg tttatttttt agggaaccta gatgttgttc
      181 tatagtcaac atatctaata atgcctgagt gggatgctca tgagcaccat cgcctgcatt
      241 gacaataatc gcttggctat tatcggctaa gaattgtgga acgcccgttc ctttatgcct
      301 tacaaccact agatcaattt tcatagcttc aatatttcgt aacgtatcta aaagtgtttc
      361 gcctttttta acgcttgaat ttgaagaagt gaaattaatc gtatcggctg aaagcctttt
      421 ttcagcaagt tcaaaactgg ttctagtccg agtactattt tcaaaaaaca aattgacgac
      481 cgtgactcct cgtaatgaag gtaccttctt cactggacgc tctaacacct gacgaaaatc
      541 catggccgta tctaatatgg ttctaatatc tgattgagat actcctcgta agccaaagag
      601 atgttttata tgcaatgcac tcatacttca ccctccactt cgactagata tacggagttt
      661 tcattatcca taggttctat agaaacgcgc acttcttgat tcacagcggt ttcaacaact
      721 aaaccgacac aatcgggggc tatcggtaat tctcgatggc cgcgatcgac aagtacacaa
      781 agacggatcg cagatggacg acctaaatcc aaaatggatt gcattgccgc gcgaacggaa
      841 cgccctgtat acaaaacatc atcgacaaga ataatttctt tatcttcaac agacgtgggc
      901 atcgccttga ttctcatttc cgtattttga atacgtttac ggtaatgaaa atcatctcta
      961 taaaaagtgg catcaatgct tcccaaaggg attttttgtc catacttttc ggataatcgg
     1021 gaagctaatt tttctgcaag tggaattcct cgtgtcgcca ttcctatcac aatcaggttt
     1081 tctggcgaag gatgcatttt ggctaattta gcggccattt catctaaagc ccattccatg
     1141 gacttagcat ctaataagag tcggatgcgt ttacaattat ctttcaaaag gggctccttt
     1201 tatacagtgc cttctgaaca catcaattta gattttttaa ctgtcctctg taaaaaaatc
     1261 ttataattat tacgatttta atgatcaaaa gcgattcaag gatcatcccc tctcttcttt
     1321 catatttata tatttaagaa atcatttaaa atttggagat tttatgttta atcagttaga
     1381 taaagctcaa agcggcgaaa ccatcgctat tattaaaacc aatcatggtg aaatgcgtgc
     1441 ccgtctattc cctgaaattg tcggagaatc cgccgaaaac tttattaaac tagctcaaca
     1501 gggtaagtat gataatgttc ctttccaccg tatcatcaaa ggttttatga ttcaaggtgg
     1561 agatttcacc aaccgtaatg gtactggtgg tcatgccgca aaaggccctg gatgcaatat
     1621 tggcgattgt tacgatgctc gtttaactca tcttcgcggg gctttaagtt gggccaaaac
     1681 atctttgcct aactctattg gaagtcagtt ttttatcgtt catcctgaag aaggaactca
     1741 tttcttagat catccagcaa atggcggagc agcggaaggc tattctgttt ttggtcaatt
     1801 atttgacggt tttgatgttc ttgatgccat tgcctctgtc aaaacagatc gaaacgatcg
     1861 tcctcacgaa gacgttttaa ttgaatccgt tgtaattgaa aaaatggcat agttttttgt
     1921 tgaccctccc ttgacaaaaa atcttttttt tgctcatatt gtgttctatc atggggttat
     1981 agctcagttg gtagagcatc acgctggcag cgtgagggtc tgcggttcga atccgcatag
     2041 ctccactaaa ataaaaaaag gactacatag tccttttttt ttatttctat attaaactca
     2101 acattcagtt ttttcgggga acccacatgt ttcggttatt ttttctagtt ctttttgtag
     2161 gggcttcatt tgctgcagat tactcccctt ttaataaaga agcttatcta acttcttctt
     2221 ttgctgaaaa tagaggaagc cgttaccacg ctggaattga tttttctact gaaatggaag
     2281 aaggttggcc catttttgct cctgaagacg gagagattca agacattaaa gtatctcctt
     2341 ttggctatgg taaagtcatt tattttaagg gtgagagtgg aaaattatgg gtttttgctc
     2401 atttaagtag ttttaatgag tcgatcgaaa atctcgtggc gcgtcggcaa tcttctgaac
     2461 gcaaaaacga tgtttctatt attctacctg acttagaccg tataaaaaag ggagataccc
     2521 ttgcttattc tggaagtact ggtattggag ctcctcattt acatatagaa acccgaatca
     2581 ctaagaataa aattgtttct ccttgtcaaa atggagtaca atgtcttgat actatagctc
     2641 cttttatttt aggagccgct gcttggcgaa atcatgaagt gattatgacc aatgaacaat
     2701 ctctccaaga gggttgccta gcagttccta acgaagacaa accattaaaa cttgctttta
     2761 aaatcgtaga ttacagccgt actccacttg aaaacccaat gtccattaga cgcattgaat
     2821 taaaagtgga aaagcgttct ttatacaaac gagttaaaga tgtcctatcc tactccacca
     2881 tgctaaaaat tagagatgaa cttctctggg ctgaagaagc agatactgct ggggactggc
     2941 atttaatgct tggagctcta cctacagaaa gaaccattcg tcttgaagta gaagattttt
     3001 caggcaatat ttcatttaga gattttactt ttcaaccatc gtgttccaca aacaccaaag
     3061 caagctattt taagcaacaa gactctactc tatttacgtt tttatctaga tcgttcttaa
     3121 atttaagcga atgttctaaa actcaatttg agctaaaaga tgctcaaaat aaaacactcg
     3181 ctactaattt atgccatatt tatcctaatg tagaaacccc tatttctctc ttttttgaaa
     3241 agtacccctc cgccgctaaa atttcagtca agcgatttaa tggaaagggg aatcgtgaaa
     3301 ttttattatt cccactaagt ggagaacttc agtctaaaat tgaaattcaa aaaccgtatt
     3361 tattaacctc agaaattaaa ggcttaaaaa acaaatacat caatcaagtg attgctattc
     3421 aagaaacaaa aaaagacagt attacttcct tagaattcca tccaaaaggg cttcaattct
     3481 ttaaaaactg gcatgtctgt tttgatacgg cttccgttaa agatcctatt tactggctcg
     3541 gtgaaacttc aagagaatgg tttgtctttt ctaagcaaac caaagaaaaa aataaacgct
     3601 gtattcaaac aaatgagctt cgtgatattg cctcgattag agatacaatt cctccagccc
     3661 caggaaaacc ttattataag gatgaaaagc ttcggattcc tgtgattgaa caatatgcgg
     3721 gtattctaaa cggaaacgcc tttaaggtga attccacaga agagccttgg attaccgccg
     3781 aatatgattc tgatcccaaa gaattaacca taaaaaaaga tcttttacct cctgcaggag
     3841 actctatctc tgtatttatg caagatgtgc ttggaaactc aaatacctat tcgattatca
     3901 ttcctgaata agtttacaaa tccttatacc tgagccagtg actctggttt aaggctgatc
     3961 gaattaagtt gtgaatctgt tctctgtggg taggaaaatc cactggatgt aacgcaattc
     4021 ttggaataat aaaagggatt ctaagcaaga gatggccaaa aaagaaggcg atcggtacta
     4081 atggcttaac taatccagca aaactaatcg ctactgaaaa gtaaattttc ttctttgtac
     4141 gaatagtgaa acgcccctca taacgcatct cttgcattaa cacctgagaa gctaagaatg
     4201 gattatcata ccaagcagga gcaataaaca cttctggaga atctaagtcg agaaagaacc
     4261 aaaaatctaa agctttttgt aaaagaatta aagaatcttt ttgatttaag ccagcaaatt
     4321 cagcttctct gtgagttaaa atctgtgcta tttgccctaa ataagaacgt tgatgagatt
     4381 cagtagatag atgggaacaa ccgtgcaata aaatttcgta accatcttcc ctccactctt
     4441 taagctgctt tttaaaagcc ttgattcgct ccacagaagc atttttagta tctggaataa
     4501 tcaatagagc aaacgggcac cccgctaaaa ggcgtatttc ttcgagcaat agagatatcg
     4561 tatcgaaatt ttcaatacta atatcatgaa agctcaacaa gcattttttt ttcttattcc
     4621 acatattgtc ttaaggaatt taattaaaaa tactagaagt tgcattcatc tttttgattt
     4681 aacagcaaga ataaaagccc ccatattaga tctattttta ttacaaggca attttaatat
     4741 ttagaatttg ctagttttt
//
LOCUS       DAOOBB010000154         4752 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_20339_length_4752_cov_4.184373, whole genome shotgun sequence.
ACCESSION   DAOOBB010000154 DAOOBB010000000
VERSION     DAOOBB010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20339_length_4752_cov_4.184373"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2140
                     /locus_tag="PLT31_07265"
     CDS             <1..2140
                     /locus_tag="PLT31_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-beta-glucanase"
                     /protein_id="HPW94970.1"
                     /translation="YGATFEVIDVSSLWKAHGNGMGRQTGEKLAVDPKKSEIVLCGTR
                     RDGLFKSTDAGKTWQKISSIGEASGADLLNQNGISFVFFDSTSAATTSGATSVIYMGV
                     SDTTSNLYKSTDGGSTWNPVSGGPASMMPHRVTMLNGNLYITFSNNTGPHNITQGAFY
                     KYNTVSGVWTNLTPKEGDTYLGSGGQSYAHGFGGVSIDPADENHILISTLNYYGGQSR
                     YATGEQGWGDRIYITKDGGQTWKTTFTYAETANAATANVGVKDNNWIAGHAIHWAGCI
                     TFNPFNTNEAWVTSGNGVFRSQNVNAEKPIWNFDAKGIEETVPLDIVSVPGGPLVTAI
                     GDYDGSSFTDIFKSTPVHSPSIGTTHSLGYAPLNGSFLRVGRVTDYSTGTGIDHNKMY
                     FSEDNAKTWTETPEPKGAHGFVALSADAASWLHRLDNSDMVYYSLDKGTTWSAVTGLD
                     AQAQYAKIVPDPVNPNVFYVLDQQGKLKKSTDQGKTFAVVGSVQNDAQGLYQASNGYI
                     RTVPNREGHIWAPLDQEQSWAANGKFSTNGLALSEDGGATWQRFPSVYAALAVGIGKA
                     APEASYETIFIWGVAGSSTNPLGIYRSIDKGASWVRINDDMHQYGGPGNGAFVQGDMN
                     VFGRVYMGTVGRGLVYGEIAGTTSAPKVKIQQQRPSATLSYRQLSLYVETAVHAQLQL
                     FNLQGKLLYTQAISSSTEVSLQPWKGKGVL"
     gene            complement(2385..3758)
                     /locus_tag="PLT31_07270"
     CDS             complement(2385..3758)
                     /locus_tag="PLT31_07270"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HPW94971.1"
                     /translation="MVNLILCGGSGTRLWPISRTLMPKQFARLFNGTSLFQKTVETNS
                     KLCSSQFIVSNADQYFLAKDQIGANYLKTAKFMLEPVGRNTAPAIALACLNLDPNAIV
                     LVTPSDHVIKNKEAYIKVLERARTLASDGYLVTFGIKPTAPETGYGYIESNGEDVVRF
                     VEKPNAEQAEKYIEAGNFLWNSGIFCFKASTFLDELQKYSPEILKASKIASNNATVED
                     SLYRVHIEDMALIPANSIDYAVMEKSKLVKVVPSDIGWSDLGSFDSLYNEFSHDENGN
                     NVNPKHLSVDSKNSLVIGSQRVIATIDLDQMLIVDTPDALLVAPLKSSQKVKNIVDQL
                     KKVNSDLPKVPQTVNRPWGTYSVLESSSRYKMKRIEVKPGERLSLQKHLHRSEHWVVV
                     SGTATVTVGGKVFLVRPNESTYIPAGEVHRLQNEGRLPLVIVEVQVGEYTGEDDIFRV
                     EDDYKRS"
     gene            complement(3772..4215)
                     /locus_tag="PLT31_07275"
     CDS             complement(3772..4215)
                     /locus_tag="PLT31_07275"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HPW94972.1"
                     /translation="MKNILVLCTGNICRSPTAHYLLQEKLGDGYEVKSAGLGALVDHS
                     ADEMAIKVALDHDLDMSNHRAKQVTDELLRWADLVLVMEAGHKNELLSKYPWLDGKIF
                     RYGDPVQVDIPDPYRRPESAFLMTWNFIAKFTPYWVERIKTTSLK"
     assembly_gap    4500..4599
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1413 a          901 c          923 g         1415 t
ORIGIN      
        1 ttacggtgct acttttgagg tcatagatgt ttcgagcctc tggaaagcgc atggtaacgg
       61 tatggggcgc cagacaggtg aaaaacttgc cgttgaccca aaaaaatcag agattgtttt
      121 atgtggtacg cgtcgagatg gtctttttaa gagtacagat gctggcaaaa catggcaaaa
      181 aataagttcc attggagagg ccagtggtgc tgatttatta aatcaaaatg gaattagttt
      241 tgtctttttt gattccacaa gtgcagcaac aacctcagga gccacaagtg taatatacat
      301 gggcgtttct gatacaacaa gtaatcttta taaaagtaca gatggtggta gcacttggaa
      361 ccctgtttct ggaggccctg caagcatgat gcctcatcgt gtgactatgc ttaatggtaa
      421 tctttatatc acttttagta ataacactgg acctcataat atcactcaag gtgcttttta
      481 taaatacaat accgtttctg gtgtatggac taacttaacg cctaaagaag gagatactta
      541 tttaggtagt ggaggacaat cttatgcgca tggttttggt ggcgtgagta ttgatcctgc
      601 cgatgaaaat cacattctca ttagtacctt aaattactat ggcggtcaaa gccgttatgc
      661 gacaggtgaa caaggctggg gcgatcgtat ttacataaca aaagatggtg ggcaaacttg
      721 gaaaacaact tttacttatg cagaaacagc gaatgctgca acagcaaacg taggtgttaa
      781 ggataataat tggattgctg gccacgcgat tcattgggca gggtgcatta cttttaaccc
      841 ctttaataca aacgaggctt gggtcacttc tggtaatggt gtttttcgta gccaaaatgt
      901 aaatgcagaa aagccaatat ggaattttga tgctaaaggc attgaagaaa cggttcctct
      961 tgatatagta agtgttcctg gaggcccttt agtgactgcg attggtgact atgatggtag
     1021 ttcttttacg gatattttta aatcaacacc tgtgcattcc ccaagtattg gaacaacgca
     1081 ttctttaggt tatgctcctt taaacggatc atttttacga gtcggtcgcg taacggatta
     1141 ttccacagga acaggaatcg atcataataa aatgtacttc tctgaagaca acgcaaaaac
     1201 atggactgaa acaccagagc ctaaaggagc tcatggtttt gtagcccttt ctgcagatgc
     1261 ggcttcttgg ttacatcgtc ttgataattc cgatatggtg tattattctc ttgataaagg
     1321 aaccacatgg agtgctgtca caggtttaga tgcacaagca caatatgcaa aaatcgttcc
     1381 agatccagtg aatccaaatg tcttttacgt tttagaccag cagggaaaat tgaaaaagtc
     1441 aacagaccaa gggaaaacat ttgctgtagt cggttctgtt caaaatgacg ctcaaggttt
     1501 ataccaagcc agtaatggtt atatacgtac agtgccaaat cgtgaaggcc atatatgggc
     1561 tcctttagat caagaacaat cttgggcagc caatgggaaa ttctctacaa atggcttagc
     1621 cctttcagaa gatggtggtg ccacatggca gagattccct tctgtatatg ccgctttagc
     1681 tgtaggcatt ggaaaagcgg ccccagaggc ttcttatgaa accatcttta tttggggcgt
     1741 agcaggatct tcgacgaatc ctcttggaat ttatcgttcc atcgataaag gcgcttcttg
     1801 ggttcgtatc aatgatgata tgcatcagta tggtggccca ggaaacggag cctttgtaca
     1861 aggagacatg aacgtctttg gccgtgttta catgggtaca gtaggccgtg gcctagtgta
     1921 tggcgaaatc gcaggaacaa catctgctcc aaaagttaaa atccaacaac aacgtccatc
     1981 ggccacactt tcttatcgtc aattgagttt atatgtagaa acggcagtgc atgctcaact
     2041 acagcttttc aatttacaag ggaagttgtt atacactcag gctatttctt cttctacaga
     2101 ggtctcttta caaccttgga aaggaaaagg agtcttgtag ctcaattagt ttctaatcaa
     2161 ggccgaagac tctcattcca aaaaattaac tgtaattaag aactaaaatg atagcgatta
     2221 gaataaatcg ctatcactcc aatgcaatag aggtgttttt ttttgattga actatactta
     2281 tgaattcgta ttgtattttc tctttttttt acaacattat ttatttaaat aaaaaaaaca
     2341 cctttcaaaa aaaggtgttt ttaagcttta aactgtacaa aaaattaaga acgcttgtaa
     2401 tcgtcttcta cgcggaagat atcgtcttcg ccagtgtatt cgcccacttg cacttccaca
     2461 atcactaaag gaagacggcc ttcgttttgc aaacgatgca cttcacctgc aggaatataa
     2521 gtagattcat tagggcgcac taaaaagact tttccgccaa cagtcactgt cgccgtaccg
     2581 ctcactacta cccaatgctc ggaacgatga agatgctttt gtaaagaaag gcgttcgcct
     2641 ggttttacct caatgcgttt cattttatag cgactacttg attctaaaac agaataagta
     2701 ccccaaggac gattgactgt ctgcggaact tttggcaaat cagaattgac cttcttaagt
     2761 tgatccacaa tatttttcac tttctggctg ctctttaatg gagcaacaag aagagcatct
     2821 ggagtatcta caatcaacat ttgatcaaga tcgatcgttg caatgacacg ttgagaacca
     2881 atcaccaaag agtttttgga atccacagat aagtgtttcg gattcacgtt attcccattt
     2941 tcatcatgag agaattcatt gtatagagaa tcaaaagaac caagatcaga ccagccaata
     3001 tcactaggaa cgactttcac tagtttagat ttttccatga cggcatagtc aatcgaattt
     3061 gctggaatca aagccatatc ttcaatatga acgcgataca aagaatcttc aacagtagca
     3121 ttattgcttg caattttaga agcttttaaa atctcaggag aatatttttg taattcatct
     3181 aagaatgtag atgccttaaa gcaaaagata ccgctgttcc ataagaagtt gccggcctcg
     3241 atatattttt cagcttgttc tgcatttggc ttttctacaa atcgaacaac atcttcaccg
     3301 tttgattcaa tatacccgta acctgtttct ggtgccgtag gtttaatacc aaaagtgact
     3361 aaataaccat cagaagcaag agtacgagct ctttcaagaa ctttaatgta ggcttcttta
     3421 tttttaatca cgtgatcgga aggggtaaca agaacaatgg catttgggtc taagtttaag
     3481 caggcaagag caatggctgg ggctgtattt ctgccgacag gctctaacat gaatttagct
     3541 gtttttaaat agttagctcc gatttgatct tttgctaaaa aatattgatc ggcattacta
     3601 acaataaatt gagaactgca taatttcgag tttgtttcta cggttttttg gaacaaagaa
     3661 gtaccgttaa ataaacgggc aaattgttta ggcattaacg tacggctaat tggccataaa
     3721 cgggttccgc taccgccgca gagaattaga ttcaccataa aaaagtcctt attattttaa
     3781 agaggttgtt ttaatacgct caacccaata tggcgtaaat ttagctataa agttccatgt
     3841 cattaagaaa gcactctctg gacggcgata aggatcgggt atatccactt gaacagggtc
     3901 gccataacgg aatattttac catcgagcca tggatatttt gacaaaagtt cgtttttatg
     3961 ccctgcttcc atcactaaaa cgagatctgc ccaacgcaac aattcatcgg tgacttgttt
     4021 tgcacggtgg ttagacatat ccaaatcatg atctaaagcc actttgatcg ccatttcatc
     4081 agcagaatga tccacaagag caccaagacc tgcgcttttc acttcgtaac catcgccgag
     4141 tttttcttgt aataaataat gagctgtagg acttctacaa atattgccag tacataaaac
     4201 taaaatattc ttcatgatgt ttaagataac aaatttctca ataaaaaaat atatatgggg
     4261 gttgggggct atgctatttt cacgaaaaaa tattcttacc ataaaaaaca ccctttgttg
     4321 ttttacaaaa ggtattcatt gaaaaaacta gttaaaatat gtataggttt ttcaaaatta
     4381 caacgacttg atttcttctt cggagaggcc tgaaatggag ataatatcag aaataggaat
     4441 gccttttttt agcatgcttt tagccatttc acgtttagcg ttaagtgctc cttgttcgan
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt accttgttcg ataccttgtt
     4621 cgataccttg ttcgatacct tgttcgatac cttgttcgat accttgctta agaccatccc
     4681 taataccttt ttcaattcct tctttgtagc tatcatcaag ccgacattcg atttctgctt
     4741 ctgtaatcat tt
//
LOCUS       DAOOBB010000155         4707 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_20571_length_4707_cov_5.078676, whole genome shotgun sequence.
ACCESSION   DAOOBB010000155 DAOOBB010000000
VERSION     DAOOBB010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20571_length_4707_cov_5.078676"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1385
                     /gene="thiC"
                     /locus_tag="PLT31_07280"
     CDS             <1..1385
                     /gene="thiC"
                     /locus_tag="PLT31_07280"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HPW94973.1"
                     /translation="HRPLRAKQGKHITQMQYAKKGIITPEMEYVAIRENQRLDELNLG
                     LGTPITPEFVRQEVAAGRAIIPANINHPECEPMIIGSSFLVKINSNIGNSAITSSIEE
                     EVEKMVWSVRWGADTVMDLSTGKNIHETREWIIRNSPVPIGTVPIYQALEKVNGKVEA
                     LSWELYRDTLIEQAEQGVDYFTIHAGLLLSHIPLTEKRMTGIVSRGGSILALWCMKNK
                     EENFLYTHFREICEILAAYDVAVSLGDGLRPGSIADANDAAQFAELDTLGELTKIAWE
                     YGVQVIIEGPGHVPMHKIEENMARQVEKCHGAPFYTLGPLATDIAPGYDHITSAIGAA
                     QIGWYGTAMLCYVTPKEHLGLPNRDDVREGVVTYKLAAHAADLAKRHFAAQFRDDMLS
                     RARFDFRWNDQFALSLDPEKAISFHDETLPGHQGKKSKFCSMCGPNFCSMRLTQAVRE
                     FVSTGTVSDF"
     gene            1552..3786
                     /locus_tag="PLT31_07285"
     CDS             1552..3786
                     /locus_tag="PLT31_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPW94974.1"
                     /translation="MSFFAYGHRKISPKVIGVTLFCVTILLLAEWTAFYRLKREHHEE
                     MKSILIDFASKDAQLLSFKIERRIEFLDKISKIENISSNNFLNYYNESDALTSFYAKG
                     WFSPNGKSTFVKAGNTFNPKLSEKTIQHFLDEDEAMAFVDIEGRSFLGLSKAVYNKQN
                     QKEGVLAAFLADTMLHSIPFERQTAIVLLNGDGKIIKDMTLTNSTKAKTPSELMVEKA
                     LFEALEKDSPSFNKKEKGGILLTPAKTNSQYYAIYVPLGFHDWSVVSMFTADVIEGSL
                     RHLNYLFKGLFIFSGLLLCGLLLYSYLLRRYYHFDLEKMISYDRLTGALTKNKLFWIL
                     KTSLKPIPGYQEALVVTNIRNFGLFNTIFGREASDSILQRSKILFEQKLKKNEWLARA
                     FADRFYLILYYKEETDLIGRLEAMSKDLQINNEDITGTYPMHYCFGVFKVPKPYKKEY
                     PEMFDLADMALKRGLGNGSKNIFFYDQMIQKSIEKEMTIESRMEEALIKKEFLMYLQP
                     KFDTITEQIVGAEALVRWQTPNEMLFPDSFIPIFERNGFISKLDMYMLEQVCLLQRRR
                     LNENKTAVPISVNQSRLLLYNPSYVDTLIDLVDRYEIPHHLIELEITETVVFEDKLVA
                     IKTFRQLLDRGFKISMDDFGSGYSSLNMLKEIDVSAIKIDRAFLENFMNSDRGRKVMF
                     SVLGLIRELNLMSVAEGVETKEQLDFLKNAGCHWVQGFYFSKPVPVSEFLKKLDIDTL
                     INVL"
     gene            complement(3773..>4707)
                     /locus_tag="PLT31_07290"
     CDS             complement(3773..>4707)
                     /locus_tag="PLT31_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HPW94975.1"
                     /translation="LSPTGQWLKKLYNTKANPKPNSKRGILIDRFSIFDILPISDQIP
                     VESFTVITGESGSGKSSLLFKHLIPRFEQGDFQSLGIEAISFLSTGNFQGNRRSSVAS
                     AIQILTPLRELFAKLPESKIRGYNASRFGFHVPGGRCETCKGEGVILDPLGYQETTCP
                     ICQGKRFREDVLEVRFKTLSFAEILSLSVERAYSLFESFEPITSKLEPLIKTGLEYLE
                     LGQPTTHLSSGERARLRLSINLSKAKMPKTLYIFDEPARGLHEKDILFMLDLIDVLKK
                     AGHTIIAIEQAKDFLIRADHILTLKRPTFKTKEH"
BASE COUNT         1478 a          757 c          955 g         1517 t
ORIGIN      
        1 accatagacc ccttcgcgct aagcagggta agcatataac tcaaatgcag tatgcaaaaa
       61 aagggattat tactcctgag atggaatatg tggcgattcg tgaaaatcaa cgtttagacg
      121 aattgaattt agggcttgga actcctatca ctccagagtt tgtgcgccag gaagtcgccg
      181 caggtagagc gattatccct gcaaatatta atcatcctga atgtgaaccg atgattatcg
      241 ggtcttcttt tttggtaaaa attaactcta acattggaaa ctctgcaatc acttctagta
      301 ttgaagaaga agtagaaaaa atggtttggt ctgtccgttg gggtgcagat accgtgatgg
      361 atttaagtac gggaaaaaat attcatgaaa cccgtgaatg gattattcgt aatagtccag
      421 tgcctatagg tacggttccc atttatcaag ctcttgagaa agtcaatgga aaagtagaag
      481 ccctctcatg ggaactttat agagatactc tgatagaaca agcagagcag ggggtagatt
      541 attttactat tcatgctggt ttattgcttt ctcatattcc tttaactgaa aaacgaatga
      601 ctgggatagt ctctcgaggg ggctccattt tagctctttg gtgtatgaaa aacaaagaag
      661 aaaatttctt atacactcat tttagagaaa tatgtgaaat tttagcggcc tatgatgtgg
      721 ctgtatctct tggagatggt cttcgccctg gttccattgc agatgcgaat gacgcggcgc
      781 aatttgcaga actagatacc ttaggagaac tgacaaaaat tgcttgggaa tacggggtac
      841 aagtgattat tgaagggcct gggcatgttc caatgcataa aattgaagaa aatatggctc
      901 gtcaggtaga aaaatgccat ggagctccat tttacacttt agggccttta gctacagata
      961 ttgctccagg ttatgatcat atcacttctg ctataggagc tgcacaaata ggctggtatg
     1021 gaacggctat gctttgttat gtgactccaa aagaacattt aggtttacct aatcgcgatg
     1081 acgttcgtga aggagtggtg acatataaat tagcagccca tgctgctgat ttagcaaaaa
     1141 ggcattttgc agctcaattt cgcgatgata tgctttccag agctcggttt gacttccgct
     1201 ggaatgatca atttgcgttg tctcttgacc cagaaaaggc aatttcattt cacgatgaaa
     1261 ctcttcctgg gcatcaaggc aaaaagtcga aattttgctc catgtgtggg cctaattttt
     1321 gctctatgag gttaactcaa gctgttcgtg aatttgtgag cactggaact gtatcggatt
     1381 tttagtttct tgggtgtgat taaatcacat ttcaatctct ttatttgttt ttggtaaaaa
     1441 aaaggaatat attaatttat ataaccttta aaacccttaa aacaaactag tgcaggttgt
     1501 agtgcttttg ttttctttgt tttttaaagg acaaaagaat aaagggcgtt tatgagtttt
     1561 tttgcttacg gacatagaaa gattagcccc aaggtaatag gcgttacctt gttctgtgtt
     1621 accattcttt tgttggcaga atggactgct ttctatcgat taaaaagaga gcatcatgaa
     1681 gaaatgaaat caatacttat tgattttgca tcgaaagatg ctcaattatt atcgtttaaa
     1741 atagaacgcc gaatagaatt tttggataaa atatctaaaa tagaaaatat aagttctaat
     1801 aattttttga actattataa tgagtcggat gctttaactt cattttatgc aaaaggatgg
     1861 ttttctccaa atggaaaaag cacctttgta aaagctggaa atacctttaa tcctaaattg
     1921 tctgaaaaaa cgattcaaca cttcttagat gaagatgaag caatggcttt tgtagatatc
     1981 gaaggtcgat cttttttagg tctaagtaaa gccgtttaca ataagcaaaa tcagaaagaa
     2041 ggtgtactag ctgctttttt agcagatacc atgcttcatt cgattccatt tgaaaggcaa
     2101 acggcaatag tacttttgaa tggtgatgga aaaatcatta aagatatgac tttaacaaac
     2161 tctactaaag cgaaaacacc aagtgaatta atggttgaga aagctttatt tgaggctctt
     2221 gaaaaagatt ccccttcttt taataaaaag gaaaaagggg gtattctttt aacaccagca
     2281 aaaacaaaca gccaatatta tgcaatttat gttcctctcg gttttcatga ttggtctgtc
     2341 gtttcaatgt ttaccgccga tgttattgag ggtagtcttc gacatcttaa ttatttattt
     2401 aagggcttat ttattttttc tggcttactg ctttgtggtt tgcttttata ctcttattta
     2461 ttaagacggt attatcattt tgatttagaa aaaatgattt cttatgatcg actcacggga
     2521 gcattaacta aaaacaaact cttttggata ttaaaaacat cgttaaaacc aataccaggt
     2581 tatcaagaag ctttagtggt tacaaatatt cgtaatttcg ggttgtttaa taccattttt
     2641 ggacgtgagg caagtgattc catattgcag cgtagtaaaa ttttatttga acaaaaactt
     2701 aaaaagaatg aatggttagc tcgagcattt gcagatcgtt tttatttgat cttatattat
     2761 aaggaagaaa cagaccttat cggtcgcctt gaagcgatgt cgaaggactt acaaatcaat
     2821 aatgaagata ttacagggac atacccgatg cattattgtt ttggcgtgtt taaagttcct
     2881 aaaccatata aaaaggaata tcctgaaatg tttgaccttg ccgatatggc cttaaaacga
     2941 gggcttggta atggttctaa aaatattttc ttctatgacc aaatgattca gaaaagtatt
     3001 gaaaaagaaa tgacgattga aagccgaatg gaagaggcat taataaaaaa agagttttta
     3061 atgtatctgc aacctaaatt tgataccata acggaacaaa ttgtaggagc ggaagcatta
     3121 gttcgttggc agactcctaa tgaaatgctt tttccagatt ctttcattcc gatttttgaa
     3181 cgcaacggtt ttatttcaaa attagacatg tatatgcttg agcaggtttg cctattacag
     3241 cgtcgtcgcc tcaatgaaaa taaaacagct gttcctattt cagtgaatca atcacgattg
     3301 ctcttatata atcctagtta tgtagatact ttaatcgatt tggtggatcg ctatgaaatt
     3361 cctcatcatt tgattgaact tgaaatcaca gaaactgttg tttttgaaga taaactagtc
     3421 gcgataaaaa catttaggca attgttagac cgaggcttta aaatatcgat ggatgatttt
     3481 gggtctgggt attcttcatt gaatatgtta aaagaaatag atgtctctgc gattaaaata
     3541 gatcgagctt tccttgaaaa ttttatgaat agtgatcgag gaaggaaggt catgttctcg
     3601 gtacttggtt taattcgaga attaaattta atgtctgtag cagaaggtgt tgaaaccaag
     3661 gaacaattgg atttcttaaa aaatgctggt tgtcattggg ttcaaggctt ttatttctca
     3721 aaaccagtgc ctgtaagtga attcctaaaa aaattggata tagacacact aattaatgtt
     3781 ctttagtttt aaacgtcggc ctttttaatg ttaaaatatg atcggctcga attaaaaaat
     3841 cctttgcttg ctcaatagca atgatcgtgt gcccagcttt ctttagtaca tctattaaat
     3901 caagcataaa taggatgtct ttttcgtgta atcctctagc gggttcatca aaaatataaa
     3961 gggtttttgg catttttgct tttgaaagat taatggataa gcgtaatctc gcgcgttcgc
     4021 cactcgaaag atgtgtggtg ggttgcccaa gttctaggta ttctaaacct gtttttataa
     4081 gcggctctag tttagaagtg ataggctcaa aagattcaaa aagagaataa gcgcgttcca
     4141 cagaaagaga gaggatttct gcaaaagaga gcgttttaaa acgtacttct agtacgtctt
     4201 ctcgaaagcg tttcccttgg caaatgggac aagtggtttc ttgatatcct aaaggatcga
     4261 gaatgacccc ttctccttta cacgtttcac agcgcccccc tggaacatga aaaccaaagc
     4321 gagaggcgtt ataacctcta attttacttt ctggtagttt tgcaaatagt tctcgaagtg
     4381 gagtgagtat ttgaatggca cttgcgacag agcttcttct gtttccttga aagttcccag
     4441 tagataaaaa tgaaatggct tcaatgccta gagattgaaa atcaccttgt tcaaatcgag
     4501 ggatcaagtg cttgaaaaga agagaagact tgccacttcc tgattctcca gtaatcacgg
     4561 taaaggactc gactggaatt tgatcgctaa taggcagaat atcaaatatc gaaaaacgat
     4621 caattagaat tcctcgtttc gaatttggtt taggatttgc ttttgtatta taaagttttt
     4681 ttagccattg gcctgtaggt gaaagag
//
LOCUS       DAOOBB010000156         4694 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_20643_length_4694_cov_4.410864, whole genome shotgun sequence.
ACCESSION   DAOOBB010000156 DAOOBB010000000
VERSION     DAOOBB010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20643_length_4694_cov_4.410864"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="PLT31_07295"
     CDS             complement(<1..438)
                     /locus_tag="PLT31_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94976.1"
                     /translation="MSKKHYLYVITIFISFCISLSYAQDSSSSSEESGYLGSLCKIPN
                     NLYAPVSSLKGIHQNLYSLMPYISNSDNIYVSDCSCKKDGSFVCTYKNLGLGQGYTPS
                     YVSLCDLREAYWCPQIEKGVCNFTGSSVAIHYSDRYNSSGYTWG"
     gene            complement(697..769)
                     /locus_tag="PLT31_07300"
     tRNA            complement(697..769)
                     /locus_tag="PLT31_07300"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(734..736),aa:Gly,seq:ccc)
     gene            989..>3171
                     /locus_tag="PLT31_07305"
     CDS             989..>3171
                     /locus_tag="PLT31_07305"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HPW94977.1"
                     /translation="MIDYLLLFAGTCFMTFADILELVRQSATEKEKGNKFEKLMKRFF
                     QNDNRYKGLLSDVWLWDEFPYKEDLGGRDLGIDLVAKTNEDTYWAIQCKCYAKETVID
                     KKAADSFIATSSRSFGEKGKLKFALRVWVSTSNKWGANAEETLNNQNPPVTRLTTADL
                     DESSLDWNALWQGKTEIKHKTKKPMDHQKEAVQKANDYFKDHDRGKLIMACGTGKTYT
                     SLLITESMVKKDGFVLFMVPSIALLGQALNAWMADTSKDIYPIGVCSDATASEIKKNA
                     KEDFDEIEDNLSELSIPASTNVKTIIKHIELARAQKKLPVIFSTYQSIDVVSQAQKAI
                     LKETKNSFGAFDLIVCDESHRTTGVKYGSVDESHFVKIHSDQFIQGHKRLYMTATPRV
                     YGENAKSKAKEQDDCILFSMDDTKVFGEEFYRVGFGYAVDKGLLTDYKVLVLSVNEYD
                     LPPSLLTTLKNNESKEFDFDDSSKMIGVISALSKLIQGDDGVTWNADPGVMKRAVAFC
                     QKIGKPNMPSSSKNFEKVMPYISEQYEKDLKNNSKNHLVNIEARHVDGSMSASARTST
                     LDWLKEETESNTCRIVSNVRCLSEGVDVPALDAVIFMSPRNSQVDVVQSVGRVMRTFK
                     KGSPNEKKYGYIIIPVVVPLDQTADEALNDNKRFKVVWDILNALRSHDDRFNAEVNSV
                     LLNKKTPPRIVAVKPAAQHKRQPFGSRNNDEPIPFDPSQIEMRFH"
     assembly_gap    3172..3271
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3281..>4694
                     /locus_tag="PLT31_07310"
     CDS             3281..>4694
                     /locus_tag="PLT31_07310"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HPW94978.1"
                     /translation="MTRVIKELRKEGVDQEVDELEKFYSSVRTNVGQIDNLEGKQKVI
                     KELYEKFFKSAFPTTVEKLGIVYTPIECVDFIIHSVNILLHEEFHTDLTNRDVHILDP
                     FTGTGTFITRLLQSGLIKKEDLERKYKKELHANEIVLLAYYIADVNIESVYHYLNKSK
                     EYLPFNGICLTDTFQTAEHKGSSQESAKLDDYFDENYKEVEKQRKTPVRVIIGNPPYS
                     VGQKSANDNAQNLSYPILDARIEDTYAAKSSANLKKSLYDSYIKAFRLASDRIQKNKE
                     GGIVAFITNGSWLDGNGQDGMRKSLVEEFTSIWVLNLRGNCRTSGEMRKKEGDGIFGL
                     GSRTPVTITFLVYNPSKKQEPCKIHYHDIGDYLTRERKLEILKEASSIQQIKWESITP
                     NEKADWINQRGDVFDTLTPLAPEKKFDTGTKSLFVVNSLGLNTNRDVWVYNHNTRNLL
                     SKMKSTITFYNSLIEDNTLGK"
BASE COUNT         1584 a          938 c          887 g         1185 t
ORIGIN      
        1 accccatgtg tatccagatg aattgtacct gtctgaataa tgaattgcta cacttgaacc
       61 agtaaaatta catacacctt tttctatttg aggacaccaa taagcttctc ttaaatcaca
      121 taaagagaca tacgaaggcg tataaccttg tcccaagccc aagtttttat aggtgcatac
      181 gaatgaaccg tcttttttac aagagcaatc ggatacataa atgttatcag aattagaaat
      241 gtatggcatt aatgaatata agttttgatg aattcctttt aaagaagaca caggagcata
      301 taaattattt ggaattttgc ataagctacc gagataaccg ctttcctctg aagaactaga
      361 tgagtcttga gcgtatgata gagatataca gaaagaaata aaaatggtta ttacgtataa
      421 ataatgtttt ttactcataa ggattccccc cccaaatgaa agttttttga tttaagtaaa
      481 taatacatta ttccttatga ctttgcaaaa gcatttactt actattcctt tattcaaggg
      541 atttttttct tttactaaca aaccaccaca ctttctacag gatatctcta aaaattgctt
      601 tgatgaaata ataagcgagc agcttgctct taatgtgaaa attgacaaaa ttatcccaaa
      661 acaaaaaaat cgcccacatt gaggcgattt ataaagagcg ggaaaagagg ttcgaactct
      721 cgacattcag cttgggaagc tgacgctcta ccaactgagc tactcccgca tgttggttga
      781 acgagtcgta atataacaaa tttaagacaa aaatggaaag cctttgagca gaaaaaaagc
      841 ccttatgagc caactaatgt tcagcgatct ctaaaaatag attaacatgc taaatgatta
      901 ccataaaaac gcttgcgaat tcatgagcaa atggccgttc gtaaaagacg caaaaaaaaa
      961 cctttgtctt atttttttat ataatttgat gattgattac cttcttcttt ttgcgggaac
     1021 ttgttttatg acctttgccg atattcttga attagtccgt cagtctgcca ccgaaaaaga
     1081 aaaggggaat aagtttgaaa agttaatgaa acgttttttt caaaacgata accgttataa
     1141 aggcttactc tccgacgttt ggctttggga cgaattccct tacaaagaag acttaggtgg
     1201 gcgtgattta gggatagacc ttgtcgctaa aacaaacgaa gacacttatt gggcgatcca
     1261 atgcaaatgc tacgccaaag aaaccgtcat agacaaaaaa gctgccgatt cctttattgc
     1321 cacctcaagc cgtagttttg gagaaaaagg caaattgaaa tttgcacttc gtgtatgggt
     1381 ttcaacttct aacaagtggg gcgcaaacgc agaagaaacg cttaacaacc aaaaccctcc
     1441 tgtaacacgc ctaactaccg ccgatctgga tgaatcctct ttagattgga atgccttatg
     1501 gcagggcaag acagagatta agcataaaac aaaaaagccg atggaccatc aaaaagaagc
     1561 ggttcaaaag gctaacgatt acttcaaaga ccacgacaga ggcaagctca ttatggcttg
     1621 cggaacaggt aaaacctata catcactcct gattaccgaa agtatggtaa aaaaagatgg
     1681 gtttgtgttg tttatggtcc cttccattgc acttttaggt caagcgttga acgcatggat
     1741 ggcagacacc tcaaaagaca tttacccaat aggggtgtgt tctgatgcta ccgcttctga
     1801 aattaagaaa aacgcaaaag aagatttcga cgaaatcgaa gacaacttaa gcgagctttc
     1861 tatacctgca agcaccaatg taaaaacaat tataaaacac attgaacttg cccgagcgca
     1921 aaaaaagctc cccgtcatat tctctactta tcaatccatc gacgtagtat cacaagcaca
     1981 aaaagcaatc ctcaaagaaa cgaaaaactc ttttggagcc tttgacttaa ttgtttgcga
     2041 tgaatcccac cgcacgacag gcgtaaaata cggaagtgta gacgaaagtc attttgtaaa
     2101 aatccatagc gatcaattta tccaaggcca caaacgccta tacatgacag ccacgccgcg
     2161 cgtatacgga gaaaacgcga aaagtaaagc caaagaacaa gacgattgca ttctgttttc
     2221 tatggacgac acaaaagttt ttggagaaga attttaccgc gtaggttttg gttatgcggt
     2281 cgataaaggg cttctcaccg attacaaagt acttgtcctt tcggtcaacg agtacgattt
     2341 accaccaagc ctattgacca cgcttaaaaa caatgagagc aaagaattcg attttgacga
     2401 ttcctcaaaa atgatagggg tcattagcgc gctttccaaa ctgattcaag gcgatgatgg
     2461 agtcacatgg aatgcagacc ctggcgtaat gaaacgagcc gtggcgttct gccaaaaaat
     2521 tggcaaaccc aatatgccaa gctcttcgaa gaattttgaa aaagtaatgc cctacatttc
     2581 agagcaatac gaaaaagacc tcaaaaataa ttctaaaaat catttggtaa atattgaagc
     2641 tcgccatgtc gatggctcca tgagtgcctc tgcacgaaca agcacccttg attggcttaa
     2701 agaagaaacc gaaagcaaca cctgccgtat agtcagtaat gtgcgttgct tgtccgaagg
     2761 agttgacgtt ccagcactag atgccgtgat ttttatgagc ccacgtaatt cgcaagtgga
     2821 tgtggttcaa agcgtaggcc gcgtgatgcg cacgtttaaa aaagggtctc caaacgaaaa
     2881 gaaatacggc tacatcatta ttcctgtggt agttccacta gatcaaacgg cagatgaagc
     2941 tctcaacgac aacaaacgtt ttaaggtagt gtgggatatt ttaaatgcac tgcgttctca
     3001 tgatgacaga ttcaatgccg aagtcaacag cgttctctta aacaagaaaa caccaccgag
     3061 aattgtagct gtcaaacctg cggcacagca caaaaggcaa ccatttggtt ctcgaaataa
     3121 tgatgaaccc attccgtttg acccgagcca aatcgaaatg cgattccatg annnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcacgcacc atgacgcgcg tcattaaaga
     3301 actccgcaaa gaaggcgtag atcaagaagt cgatgaactt gaaaaatttt attcctcagt
     3361 gcgcaccaac gtagggcaaa tcgataacct agaaggcaaa caaaaagtca tcaaggaact
     3421 ctatgaaaaa tttttcaaat cggcattccc cacaaccgtt gaaaaattag gcattgttta
     3481 cacgccaata gaatgcgtgg actttattat acattcagtc aatattcttt tgcacgaaga
     3541 attccatacc gatttgacaa accgagacgt tcatatttta gaccccttca caggcacagg
     3601 cacgtttatt accagattat tgcaaagtgg cctaataaaa aaagaagact tggaacgcaa
     3661 atataaaaaa gaactccacg cgaacgaaat agtactcctt gcctactaca ttgccgatgt
     3721 aaatattgaa agcgtttatc attacttgaa caagtctaaa gaatatttac ccttcaatgg
     3781 aatctgcctt acagacacat ttcaaacggc cgaacacaaa ggcagcagcc aagaaagtgc
     3841 aaaattagac gattactttg acgaaaatta taaagaagta gaaaaacagc gaaagacccc
     3901 tgtacgcgtg atcattggaa accctcctta ttctgtgggg caaaaaagtg cgaacgacaa
     3961 cgctcaaaat ttaagctacc ccattttaga cgcccgtatt gaagatacct atgcagcaaa
     4021 gtcttctgcg aatttaaaaa agtctcttta cgattcctat atcaaggcgt ttcgcctagc
     4081 cagtgaccgt attcaaaaaa acaaagaagg cggcattgtc gcttttatta ccaatggaag
     4141 ttggctagac ggtaacgggc aagacggcat gcgaaaaagc cttgtcgagg aattcacaag
     4201 catttgggta ttaaatcttc gtgggaattg cagaactagt ggcgaaatgc gcaaaaaaga
     4261 gggtgatgga atattcggcc ttggaagccg tacccctgta acgattacct tccttgtata
     4321 taacccgagt aaaaaacagg aaccctgcaa aatccattac cacgatattg gcgattactt
     4381 gactcgtgag cggaaactcg aaatcttaaa agaagcctcc tcgattcaac aaatcaaatg
     4441 ggaatccatt acgcctaatg aaaaagccga ttggattaac caacgcggtg atgtatttga
     4501 tacactaacg ccactggctc cagaaaagaa gtttgatacg gggacaaaaa gtttgtttgt
     4561 ggtgaactct ttaggcttaa atactaatcg tgatgtttgg gtatacaacc ataatacaag
     4621 aaatcttttg agtaaaatga aatctactat aactttttat aattctttaa ttgaagataa
     4681 tacccttgga aagc
//
LOCUS       DAOOBB010000157         4676 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_20743_length_4676_cov_4.180913, whole genome shotgun sequence.
ACCESSION   DAOOBB010000157 DAOOBB010000000
VERSION     DAOOBB010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 4676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4676
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20743_length_4676_cov_4.180913"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..1391)
                     /locus_tag="PLT31_07315"
     CDS             complement(195..1391)
                     /locus_tag="PLT31_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-2-enoyl-CoA reductase family protein"
                     /protein_id="HPW94979.1"
                     /translation="MIVKPLIRSNICLNAHPVGCKKEVERQIFYVKQQAQKREPVKDA
                     PKNVLVLGCSTGYGLASRIAAAFGYGATTIGVSFEKAGTENKSGTPGWYNNMAFDQAA
                     EKEGLKYVTYNGDAFSDEMRQTIISKAKEMNIRFDLIVYSVASAVRVDPVSGEMYRSV
                     LKPFGLSFVGQNIDVATNKLITISAETANPEEAAQTVKVMGGEDWERWIQQLSDADVL
                     ASGVKTIAYSYIGPSLSHAIYRDGTIGKAKEHLEKTADVLNEYLSSKVKGEAYVSVNK
                     GLVTRSSAVIPVIPLYLSILFKKMKEKGTHEGCIEQAERLFAERLYTGGAVPVDENRL
                     IRIDDLELDPEVQDYVSERMPLVTEENLSEIGDLEGYKHDFLATNGFDVEGVDYNAEI
                     ERMDII"
     gene            complement(1406..2662)
                     /gene="fabF"
                     /locus_tag="PLT31_07320"
     CDS             complement(1406..2662)
                     /gene="fabF"
                     /locus_tag="PLT31_07320"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HPW94980.1"
                     /translation="MNKRRVVITGLGAITSIGKSVPETWESVRRGECGIDKITLFDAS
                     THPVQIAAEVKNFNPEDHGIAKQEAKRMARFTQFLLAATKESIQDASLPEEMVTGEKT
                     GIVAGNGLSGYDVLESSFERYFKSGPKRIPPLAVPMFIPNEAAANVSIQLGINGPSWT
                     LATACASGTDALGVGLDLVRSGRLDICFAGGTEGIITGFGIGSFSVLHALSASHNDSP
                     KTACRPFDKDRDGFIMGEGAGILVLEELEHALARGAKIYGEFMGYGASSDCYHITSPN
                     PNGLGGAKAITRALEDAGVKPEEVQYYNAHGTSTQLNDATETKMIKNAFGDHAYKMKV
                     SSTKSMTGHCVGAAGAVEAIVSIKAMNESFFPPTINLDNPDIEQGCDLDYVPKVGVPG
                     NIDCFVSVSLGFGGHNGAIVMKKFVK"
     gene            complement(2688..4367)
                     /locus_tag="PLT31_07325"
     CDS             complement(2688..4367)
                     /locus_tag="PLT31_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPW94981.1"
                     /translation="MSTLLNDFLDRTEFDSYKDLRHRFSIRIPTEFNFAYDVVDKYAE
                     IDPNKEALVWCDDQGEEHIFSFKDLSEASKKTANYLTSLGIKKGDAVMLILRRRYEFW
                     FFLLALHRIGAIAVPATNMLTAKDIEYRNNSASIKMIVALDEPALQEQVESAQPKSPS
                     VETLVTIGPDRNGWESFNKAFDQQSSEFARPQGENAIHNSDIMLLYFTSGTSSYPKMV
                     QHDFTYPLGHIVTAKYWQNVEENGRHLTIAETGWAKAMWGKIYGQWIAGCAVFTYDML
                     TFIPGKVLEKLAQYKVTSFCAPPTAYRYLVQLDMSKYDLSNLHYCTTAGEALPIEVFN
                     TFLKKTGLCLKEGYGQTELTLTIGNYPWMVARPGSMGKPAPGYDIDIVHPNGVSCTAT
                     EIGEIVIRVEKGRPFGMFAGYYRDQALTDKVFAGDIYRTGDTAWKDDDGYFWFVGRTD
                     DLIKSAGYRISPFEVENVLLQHPAVLECAVTGVDDKARNQVVKATIILAEGYKASKEL
                     SIDIQSFAKHNSAAYKCPRIIEFVSELPKTISGKIRRVAIREKDRQAMLQS"
BASE COUNT         1417 a          940 c          880 g         1439 t
ORIGIN      
        1 aggattaaaa atttttgtgg gaaaaacatg tccttatttt ggctgttcga tagcgtgtat
       61 aaaaaagcca atgtaaaatg aaaagtcggc ttcaataaaa aaagtcaatt taaaatgaaa
      121 aatcgatttc aataaaaaag ccttgaaaga gattattctt tcgaggcttt ttcagaaatg
      181 atgagaataa agaattagat gatatccatt ctttcgattt cagcattgta atcaacgcct
      241 tcaacgtcaa aaccattcgt ggctagaaaa tcgtgcttat acccttctaa atcaccaatt
      301 tcagaaagat tttcttcagt gacaagaggc atgcgttctg acacgtagtc ttgaacttct
      361 ggatctaatt caagatcatc aatacgaata agtcggtttt catctacagg aacagctcct
      421 ccagtgtaaa gacgttctgc aaacaaacgt tctgcttgtt caatacaccc ttcatgggtt
      481 cctttttctt tcattttctt aaatagaata gatagataaa gaggaatcac aggaatcaca
      541 gcactagaac gagtgacaag gcctttatta acggaaacgt aagcttcgcc cttcactttg
      601 gaagagagat attcgtttag aacatcggct gttttttcta aatgctcttt tgctttacca
      661 atggtgccgt ctctataaat cgcgtgagat aaagaagggc caatatagga ataagcaatt
      721 gtttttacgc cacttgctaa aacatcagca tcacttaatt gctgaatcca gcgttcccaa
      781 tcttcaccgc ccatcacttt tactgtttgt gcggcttctt ctgggtttgc tgtttctgca
      841 gaaatggtga ttaatttatt ggtggctaca tcgatatttt gaccaacaaa agataagcca
      901 aatggtttta aaacagaacg gtacatttca ccagatacgg ggtctacgcg aacagcactt
      961 gctacgctat agacaatcaa atcaaaacgg atgttcattt ctttggcttt agaaataatg
     1021 gtttgacgca tttcatctga aaaggcatcg ccattataag tgacatactt taggccttct
     1081 ttttcggcag cttgatcaaa agccatgttg ttataccaac ctggagttcc acttttgttt
     1141 tcagtgcccg ctttttcaaa agaaacgcca atagtagtgg cgccataacc aaaagcagcg
     1201 gcaatacgac ttgctaaacc atagcctgtg gagcaaccta gaacgagtac atttttagga
     1261 gcgtctttta caggttctct tttttgagct tgttgtttta cgtagaaaat ttggcgttct
     1321 acttcttttt tacatcccac tgggtgggcg tttaagcata tattactacg tattaatggt
     1381 tttactatca tttcaggttc cctttttatt tgacaaattt tttcatgacg atggccccat
     1441 tgtgcccgcc aaagcctaaa gaaacagaaa caaagcaatc gatgttacca ggaacgccca
     1501 ctttagggac ataatctaag tcacagcctt gttcaatatc aggattgtct aagttgatgg
     1561 ttggaggaaa gaaactttca ttcatggctt taatgctaac aatggcttcc actgctccag
     1621 cagcaccaac acagtggcca gtcatacttt tagtgcttga tactttcatt ttataagcat
     1681 ggtcaccaaa ggcattttta atcatttttg tttcggtagc atcgtttaat tgtgtagatg
     1741 taccgtgagc attgtaatat tgtacttctt ctggtttaac acctgcatct tcgagagcgc
     1801 gggtaatggc tttggcgccg ccaaggccat ttgggttggg gctagtaata tggtagcaat
     1861 cgctggatgc cccatacccc ataaattcac cataaatttt tgccccgcgc gctaaagcgt
     1921 gttctaattc ttcaagaact aatatgccag caccttctcc catgataaac ccgtcacgat
     1981 ctttatcaaa aggacgacaa gctgttttag gagaatcatt atgagatgca gataatgcgt
     2041 ggagaacaga aaaactaccg ataccaaaac cagtaataat cccttctgta ccaccagcaa
     2101 agcaaatatc taatctacca gaacgcacga gatcaagacc aacaccaagt gcgtctgtgc
     2161 cagaagcaca agcagtggct aaagtccaag aagggccatt aatccctagt tgaatactca
     2221 catttgctgc agcttcgttt ggaataaaca tcggaacagc aagaggtgga attcgttttg
     2281 ggccagactt aaaatatctt tcgaaggatg attctaaaac atcataacca ctcaaaccat
     2341 ttcctgcaac gattcctgtt ttttctcccg tcaccatttc ttcaggaagg ctagcatctt
     2401 gaatcgattc tttagtagca gctaatagaa attgagtaaa gcgagccatt cttttggctt
     2461 cttgttttgc aataccatga tcttcaggat taaaattctt aacttcagca gcaatttgta
     2521 cggggtgagt agaggcgtcg aacaaggtga ttttatcaat accgcattct ccacggcgca
     2581 cggactccca agtttctgga actgattttc caatagaagt gattgcacca aggcctgtaa
     2641 taacaactcg tcttttattc ataaactctc tatatataat gtgaaactta gctttgtaac
     2701 atcgcttgtc tatctttttc ccggatggcg acacgacgga ttttcccgct aatcgtttta
     2761 ggtaattctg agacaaattc aataatacga gggcatttat acgccgctga attatgtttg
     2821 gcgaatgatt gaatatcaat ggataattct tttgaagcct tataaccttc agcaagaata
     2881 atcgtagcct ttacaacttg atttcttgct ttatcatcaa cacccgtcac agcgcattca
     2941 agaacagcag ggtgctgtaa tagaacgttt tcgacttcaa atgggctaat tctgtaccca
     3001 gcactcttaa tcaaatcatc tgttctacca acaaaccaga agtaaccatc atcatctttc
     3061 caagcagtat cgcctgtacg ataaatatca ccagcaaata ctttgtctgt taacgcttga
     3121 tctcgatagt aaccagcaaa cataccaaaa ggacgtcctt tttctacgcg aatgacaatt
     3181 tcaccaattt cagttgctgt acaagaaact ccattaggat gaacaatatc aatatcataa
     3241 ccaggagcag gtttgcccat agaacctgga cgagctacca tccaagggta atttccaata
     3301 gtaagcgtta attcggtttg cccataacct tctttgagac aaaggcctgt tttcttaaga
     3361 aaggtattaa aaacctcaat aggaagagct tcgcctgctg tagtgcagta atgtaagtta
     3421 gataagtcat atttggacat gtccaattgt actaaatagc ggtatgctgt aggaggagca
     3481 cagaaagaag tgactttata ttgtgctagt ttctcaagaa ctttacccgg aataaaggtg
     3541 agcatatcat aggtaaagac agcacaacct gcaatccatt gtccgtagat ttttccccac
     3601 atggcttttg cccatcccgt ttctgcaatg gtaagatgac ggccgttttc ttccacattt
     3661 tgccagtatt ttgcagttac aatgtgccca agagggtatg taaagtcatg ttgcaccatt
     3721 ttagggtagc tagatgtacc cgaggtgaaa taaagaagca taatatctga attatggatg
     3781 gcgttttccc cttgagggcg tgcaaattca gatgattgct gatcaaaagc tttattaaaa
     3841 gattcccacc cgtttcgatc aggaccaatc gtcacgagag tttctacact aggtgatttc
     3901 ggctgtgcgg attcaacttg ttcctggagt gctggttcat ctaaagcgac aatcatttta
     3961 atgctggcgg agttatttcg atattcgata tctttagcag tcaacatatt tgtggctggt
     4021 acagcaattg ctccaatacg atgaagcgct agtaagaaaa accaaaattc atatcgacgt
     4081 cgaagaataa gcattacggc gtcgcctttt ttaatcccaa gagaggtcaa ataattggct
     4141 gtttttttag aagcttcgga taagtcctta aaagaaaaaa tgtgttcttc tccttgatcg
     4201 tcacaccaga caagagcttc tttattggga tctatttctg catacttatc aacaacgtca
     4261 taggcaaaat taaattccgt tggaatacga atagaaaaac gatgacgtag atctttgtat
     4321 gaatcaaatt cagtacgatc taaaaaatca ttaagtagcg tagacatatc aatttccctt
     4381 ttggattatt aatatctaat gaaatgtaaa gctaggattt ttttctttct tttttaaggt
     4441 ggagataaca gaaataaata agaataaagt tttttgtttg ctaataatta ttaaatgaaa
     4501 gttcatttaa taattattag gtattgttaa aatgtgattc aataactgtt gtttttaggg
     4561 tctttcctga atttatgttc attagcgaag ccttatacta taaggcaata gaagcattca
     4621 cttctagaat cagttattct gagtttcatc cgagcgtcag ctacatcgct ggttag
//
LOCUS       DAOOBB010000158         4660 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_20824_length_4660_cov_5.756569, whole genome shotgun sequence.
ACCESSION   DAOOBB010000158 DAOOBB010000000
VERSION     DAOOBB010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20824_length_4660_cov_5.756569"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PLT31_07330"
     CDS             complement(<1..514)
                     /locus_tag="PLT31_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94982.1"
                     /translation="MKKATKILLLITFLSISFVNAETTTLQGLWKYYGNVFDGSVKRF
                     DNINISVKNDSVHFYINETQSVKGFLRNDTIFVEQGSELYFIEWIRIVNKDSLISSIS
                     ANNSIEILSFSRLDLNGEWEQHNKGRSYSEPGVDGLISTISYKDTCTIVQEKDSVFFY
                     ESSDFLAKGYL"
     gene            complement(635..1711)
                     /locus_tag="PLT31_07335"
     CDS             complement(635..1711)
                     /locus_tag="PLT31_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase"
                     /protein_id="HPW94983.1"
                     /translation="MKKQLAILSILSVTLSFAQVVSVDFENQNEGVYTNAQARNDFVP
                     LRGASSWYAMEQNKGQNAKIVLDDQEHGKVLQLKYPVGCLGPNDDPLACAGQIKQPLT
                     ETAEEMWVAYELYFEEGFDFVKGGKLPGLCGGKCYTGGNRPSIGDGWSARVMWRPDGK
                     VVQYLYFVDQAQTYGDDANWDLDGTAEQKYFQIGTWHRLVTKITLNTVTNEGHGEKDG
                     KIKSWFDGELALDLDTLRLRDYANQRIDIFYLSTFHGGSDNTWSPTVDSYIRYDHFVI
                     SKDSIPVTSSPTKMISSKQEIANKNFQIAIGNSVLLKTPLKEETCFDFYTLDGQWYKT
                     KTYAKETTRISLPNENQVFIIKTR"
     gene            complement(1898..3655)
                     /locus_tag="PLT31_07340"
     CDS             complement(1898..3655)
                     /locus_tag="PLT31_07340"
                     /inference="COORDINATES: protein motif:HMM:NF014237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HPW94984.1"
                     /translation="MGIRKTFFLVSALASMAMAATIYEAELGTLGNASDPSQNPAITT
                     DASASGGKYVKMNGGSISLTVTAAETKNYAIKIHYKNNYGGSKENILAVGSNTIGSIV
                     FPETQASTFEDVESVARLNAGQNTVSINNSWGWIDVDYIEVSDYVAEAFNISSTVVTA
                     GATEEAQKLYTFLVNNFQKKTISGVMTGDVAIGTPLKSQADVVAVHTAGGKYPVLVGF
                     DFLFATGKAGDVNSWNQDYTNETIKLAKELWDLGGIPAFTWHWKDPSNAVDAFYIEEA
                     QRDGDEFTTFDYRTGFKAGTTEWDTNSDTYKQIVEDIDKISDLFLTLQEQKVAAIFRP
                     LHECGGAWFWWSAVSKSDVHSGAEFVALYRLVYDRMVKVNGVKNLVWNWNPQTAALKD
                     WNPGEEYFDILSVDIYNEANDHASNAAAFNGLKSNISKNKILALSENGPIPDVENMHN
                     DEAVWSWWMPWYESWSGGFVSKTAASVWQSNFNDERILTLDKMPGWNGPISISKTASI
                     NPLKWSVQGKSLLIHTSKNDNVKIEVYGMNGAHIKTLAKGTLSLGSHRFSLEDLSQGL
                     YMLRVQGTMGVLNRTITIK"
     gene            3820..>4660
                     /locus_tag="PLT31_07345"
     CDS             3820..>4660
                     /locus_tag="PLT31_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02147 family protein"
                     /protein_id="HPW94985.1"
                     /translation="MLTGHQLNIFDYLDYHEFLRDFYTLEKATDPLFSYRVFAVAVHI
                     DASLLVKILQGKRHLSAEGIEQFISFFRFKSAKSEYFRELVAYAKARRDLDLKRHFEK
                     LQKMRPAAKRQIDEARYRYFQMWYFPMIRSAIDVFEYFSHEQAETLGSLCHPKLTAAQ
                     VIEALTHLEKLGLIQKDSSGRLVPTDAHLSTGERWQSAAVQDYQKQMVDHAKNAIDTI
                     PREKRDISTLTLALDSSQIERIKAVLAEARTSIVKQVGAMPSNECDAVYQLNIQLFPV
                     MQKE"
BASE COUNT         1464 a          961 c          740 g         1495 t
ORIGIN      
        1 ttaaatagcc cttagccaaa aaatctgatg attcataaaa aaagacggag tctttttctt
       61 gtactatcgt gcaagtatct ttataagata tagtcgaaat caacccgtct acaccaggct
      121 cactataact tcttccttta ttatgttgtt cccattctcc atttaaatct agcctagaaa
      181 aagaaagtat ttcaatagaa ttatttgcac taatagaaga gattaatgag tctttattca
      241 caatacgaat ccattctata aaatataatt cagatccttg ttctacaaaa atcgtatcat
      301 tcctcaaaaa gcctttcacg ctttgtgttt catttatata aaaatgaaca gaatcatttt
      361 ttacagaaat attgatatta tcaaatcttt ttacagaacc atcaaataca tttccataat
      421 acttccacag gccttgaaga gtcgttgttt ctgcattcac gaatgaaata ctcaagaatg
      481 tgattaatag aagaatcttt gttgcttttt tcataatttc cttatgatta tcctaaaact
      541 cttttttaag attaactaat gaaattcctt ttcgcaacaa aaaaagctag ttgtacaaac
      601 aaactagctt aaaatcaaaa gggattaatt tgtatcacct tgttttgatt atgaaaactt
      661 gattttcatt tggaaggcta atccttgtcg tttctttggc gtatgttttt gttttatacc
      721 actgtccgtc tagcgtataa aaatcaaaac aggtttcttc ttttagtggg gttttcaaaa
      781 gaaccgaatt tccaatagca atttggaaat tcttatttgc tatttcttgt ttagaagaaa
      841 tcatttttgt gggcgaagat gtgacaggaa tggaatcttt agatattaca aaatgatcat
      901 atcgaatata actatccact gttggagacc atgtgttatc actacctcca tgaaatgtag
      961 agagataaaa aatatcaatg cgctgattag cataatctct taagcgaagg gtatctaaat
     1021 ctaaagctaa ctctccatca aaccaacttt ttatttttcc atctttttct ccatggcctt
     1081 cgttagtcac cgtattcaaa gtgatttttg taactaggcg atgccatgtt cctatttgga
     1141 aatatttttg ttctgccgtt ccatctaaat cccagttggc atcatctcca taagtttgag
     1201 cttggtccac aaaatagagg tattgtacga ctttcccatc ggggcgccac atcactcgtg
     1261 cactccagcc atcccctata ctaggcctgt ttcctccagt ataacattta ccaccacaaa
     1321 gaccaggaag ctttccgcct ttcacaaagt caaaaccctc ttcaaaatag agttcataag
     1381 cgacccacat ttcttcagct gtttctgtga gaggctgctt aatttgacca gcacatgcaa
     1441 gtggatcatc atttggccca aggcaaccca caggatattt taattgaaga actttaccat
     1501 gttcttgatc atctaaaacg atttttgcgt tttgaccttt attttgctcc atcgcatacc
     1561 aactagaagc tcctcttaaa ggaacaaagt catttcttgc ttgagcattt gtataaacac
     1621 cttcattttg attttcaaaa tcaacagaca ccacttgagc aaaagaaaga gtgacgctta
     1681 aaatagagag aatagcgagt tgttttttca ttttaagaat ttcctaaata gatactatcc
     1741 taaaataaat aaaaaaagtg ccccatgtac cataccaagg catccaccag ctccgaactc
     1801 gtgttactag aataatcgta agcactacaa aaaataaaac ccttcataaa aaaagcccta
     1861 aaagagcttt tttatgaagg gagtgggggg aggattttta ttttatagta attgtcctat
     1921 taagaacacc catagttcct tgaacgcgta acatatataa accttgagaa agatcttcta
     1981 aagaaaagcg atgtgatcct aaagaaagag ttccttttgc taaggtttta atatgagctc
     2041 cattcatgcc ataaacttca atcttcacat tgtcattttt tgaagtatga atgagtaatg
     2101 atttaccttg aacactccat tttaatggat taatagatgc tgttttagag atggaaatag
     2161 gaccattcca gccaggcatt ttatctagag taagaatacg ttcgtcgttg aaatttgatt
     2221 gccatacaga agcggctgtt ttacttacaa aacctccact ccatgattcg taccaaggca
     2281 tccaccaaga ccaaaccgct tcatcattat gcatattttc tacatcagga atcggtccat
     2341 tttcagaaag agcaagtatt ttgtttttac tgatattact tttcaagcca ttaaaagcag
     2401 ccgcattact tgcgtgatca ttggcttcat tgtaaatgtc tacgctcaga atatcgaaat
     2461 attcttctcc tggattccag tcttttaagg ccgctgtttg tggattccag ttccatacca
     2521 agttcttgac accattgact ttaaccatac gatcataaac taagcggtaa agggctacaa
     2581 attccgcacc tgaatgtacg tcacttttac ttacggcact ccaccagaac caagcgccac
     2641 cacactcatg aagcggacgg aaaatggcgg ctaccttttg ttcttgtaaa gtcaagaata
     2701 gatcagaaat tttatcgata tcttctacga tttgtttata cgtatctgaa ttagtatccc
     2761 attccgttgt acccgcctta aatcccgttc tataatcaaa ggtggtgaat tcgtcaccat
     2821 ctctttgagc ttcttcaata taaaaagcat ctacggcatt acttggatct ttccaatgcc
     2881 atgtaaaagc aggtatacca cctaaatccc ataattcttt agcaagcttt attgtttcat
     2941 ttgtataatc ttgattccag gaatttacat cgccagcttt tcctgtagca aataaaaagt
     3001 caaagcccac tagaacaggg tattttccac cagccgtatg cactgcgaca acatctgcct
     3061 gagattttaa aggagttcca atagcaacat cacctgtcat cacaccagaa attgttttct
     3121 tctggaaatt attcacaagg aatgtatata gcttttgcgc ttcctctgta gcacctgctg
     3181 taacaacagt cgagctaata ttaaatgctt cagctacata atcactgact tcaatatagt
     3241 ctacgtctat ccaaccccaa gaattattga ttgaaactgt attctgcccc gcattcaaac
     3301 gagccacact ttccacatct tcaaaagtac ttgcttgagt ttctggaaat actatagaac
     3361 caatagtatt gctacctaca gccaaaatat tttcttttga accaccgtaa ttgttcttat
     3421 aatgaatttt tatagcatag tttttcgttt ctgcagcagt cactgtcaaa gaaatggatc
     3481 caccattcat tttcacatac ttgccgcccg aagcactcgc atctgttgta attgctggat
     3541 tctgagaagg gtcactagca ttccctaaag ttcccaattc tgcttcataa atcgtagctg
     3601 ccatggccat ggaggctaaa gcgctaacca aaaaaaaggt ttttctaata cccatgttta
     3661 ctcctcttta agtttttctt aaaaatagac ccttttctct ttaaaagggc ttaaaaaatt
     3721 tcaaaggtga tgacaaaaat gtccacacct agatattaat tttctcttat ttttagccaa
     3781 tcaaacaaaa ttgatgtaaa ttatttttaa aggtttacta tgctaacagg tcatcagctt
     3841 aatattttcg attacttaga ctatcacgaa tttttgcgtg atttttatac gctagaaaaa
     3901 gcgacagatc cgttatttag ttatagagta tttgctgttg ccgttcatat tgatgcaagt
     3961 cttctagtaa aaatactgca aggtaaacgc cacctttctg ctgaaggtat tgaacaattc
     4021 atttcatttt ttcgttttaa atcagcgaaa tcagagtatt ttcgagaatt agtagcatac
     4081 gctaaagcac gtcgtgattt agatctaaaa agacattttg aaaagcttca aaaaatgaga
     4141 cctgctgcga aacggcaaat tgatgaagct cgttatcgct attttcaaat gtggtatttt
     4201 cctatgattc gctcggcaat agatgttttt gaatatttta gccatgagca agccgaaaca
     4261 ctaggttctc tttgccaccc caaactaacg gcagcacaag tgattgaggc tttaacccac
     4321 ttggaaaaac ttggcctgat tcaaaaagat tcttctggac gtcttgttcc tactgatgcg
     4381 catttaagca caggagaacg ttggcaaagt gcggctgttc aagactacca aaagcaaatg
     4441 gtggatcacg ctaaaaatgc aatcgatact atccctcgtg aaaaaagaga catcagcacc
     4501 ctaacacttg cattagattc atctcaaatt gagcgtatca aagcagtgct tgccgaagca
     4561 agaacttcta ttgtaaagca agtaggagcc atgccctcta atgagtgcga cgccgtttac
     4621 caactcaata tccagctttt tcctgtcatg caaaaggaaa
//
LOCUS       DAOOBB010000159         4635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_20980_length_4635_cov_4.506769, whole genome shotgun sequence.
ACCESSION   DAOOBB010000159 DAOOBB010000000
VERSION     DAOOBB010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20980_length_4635_cov_4.506769"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2513)
                     /locus_tag="PLT31_07350"
     CDS             complement(<1..2513)
                     /locus_tag="PLT31_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HPW94986.1"
                     /translation="MKKKIVLIMGIILTYVANLFAAEQAVFYVAPSGSDANAGTKEAP
                     FKTIQKAQQAVRAINSSMTGDIVVYLRDGTYVLDNTLRFNNSDGGTNGFYVRYENYTG
                     EKPFITGGIPVPSWSLCDEANDIWCAEGVDARFRQLYVNGKKGIRARHPNLNSDGSHN
                     MFRLTKVDSMGRAFDVSSEYTGDWKNMNKVEIHLMIAWAESVLRLEKIERNGSVSKVI
                     PQDPERSKLFRRKYPMLGTAFMSNPPKQQTFYFENAYEFLDQPGEWYLDETTDILYYK
                     PRSGESLTSATVVAPNLETLVEIKGESTKNKVQNMKFKGLIFANSNFLRPSKEGFLNL
                     QGGQFNVEVLEENGLLGSNKFLLWRPNAGVSVTNVSHFIFERNVFTQMAATGLDFISG
                     TNDNRIEGNIFTDIGGSGISIGKFAADSTTEIHIPYNPADKDEISTRDTIRNNLVTHG
                     TTEIQGGVGIIAGYPRYIVIEHNEVSYMNYTGISVGFGWTKQQTAMTNNKINRNEIHH
                     VAQLLSDGGAIYTLSNQGTGSEIMYNYSHDISASKWSDYWTCPIYLDEGSSGFTVAHN
                     VAVNAPKGTACNQCGTNTTTDNDGNSPEVIANAGIEDSFADILSYSIPLPDFSKVTPQ
                     APFSTEIVIPGTIQNENYDVGGQRNSYYDMDSENQGAEYREDAVDIVAVTNGYAIGYT
                     SAGEWLEYSIDVKEAGAYQFRANVSTGLENSSFQLFLDNVAISDTIVVPQGEDWDTYG
                     YVEGETTKELTTGKHVLKVLVTGSYVNLDWILFAKDLNQVAIPSIRSSWNDVGNRDAV
                     LHIYNLQGVHQGSFTMNGVLTKDALISKMQEFNYKAGVY"
     gene            complement(2538..4562)
                     /locus_tag="PLT31_07355"
     CDS             complement(2538..4562)
                     /locus_tag="PLT31_07355"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HPW94987.1"
                     /translation="MKQNFRTTLLGYLAVLLLPSLGMADNPIAQAYYTPDPAPMVWND
                     TVFVYTGNDEGGSFFTMNNWRVYSSTDMVNWTDRGFVFGHESITNAKPAGDWAAECIY
                     RNGKFYYYVTVESTKGGRAIAVGVADKPDGPFKEAYNRHMAGPNWDYIDPTVFVDDDD
                     QAYIYWGNPKLYYAKLKEDMITIDGQINVTDMSRGFAPNGESSKYTEGPWIHKRGDKY
                     YMIYASHGVPEKISYSWSDSPTGPWEWKGVILDQHSGFAFTSHAGIIEFKGRAFIFYH
                     TAKLPGGGGFSRSTQVDEFTFNSDGTIPLVKPTDLGVVKPIHNLDPFKVVQAETKAFS
                     RGIGTYRVEGKGVYVNKLDNNDYIKVRSVDFGDDGADKFYAAVKGGSGTIEIRLDKQD
                     GEMIGQLNVTSANDFVEQSVDVSGAVGVHDLFFVFKGGTSMFELDYWYFETAAAAIPQ
                     GPFCGVDKTGCVATPIPGKIEVENYDVGGSNKAYYDVSIENEGAKYREDRVDIVADEF
                     GNYMIGYTEAGEWLEYTVNVEEAKAYPFRARVSSGSESSSFQLFLGDKAITDTIKIET
                     TGDWDTYAEIEGTTSAISSGEQVLKLLITGSYVNIDWIEFGEKTNSISKTFDLEKQGL
                     QTYKVFSLQGQYLGDIQSNSIQDCKREIHERFPVGKYVIRDSHHAYRVIK"
BASE COUNT         1395 a         1059 c          748 g         1433 t
ORIGIN      
        1 aagtatacac ctgctttata attgaactct tgcattttcg aaattaatgc atcctttgtt
       61 aaaactccgt tcatagtaaa agatccttga tgaacacctt gcaaattgta tatatgcaaa
      121 acagcatcac gattacccac atcattccat gaagagcgaa tacttggaat tgctacttgg
      181 tttagatctt tcgcaaataa aatccaatcg agattgacat aacttcctgt aaccaagact
      241 ttcaaaacat gtttaccagt agttaattcc ttcgtggttt caccttctac gtagccataa
      301 gtatcccaat cttctccttg tgggacaacg atcgtatcag aaatagcaac attatctaaa
      361 aatagctgga aggatgaatt ttctaaccca gtcgacacat tcgctctaaa ttgatatgca
      421 cccgcttctt taacatcaat agaatactcg agccattcgc cagcacttgt gtagccaata
      481 gcataaccat tagtgacagc aacaatatct acggcatctt cgcgatattc tgcaccttga
      541 ttttctgaat ccatatcgta atatgaattt ctttgtccac ctacatcata attttcattc
      601 tgtattgttc caggtattac tatttcagta gaaaatggtg cttgcggagt tacttttgaa
      661 aaatcaggta aaggaatcga atagctcaat atatctgcaa aagaatcttc aattccagca
      721 ttggcgatta cttcaggact atttccatcg ttatccgttg tagtatttgt tccacattga
      781 ttgcacgctg ttccttttgg agcgtttaca gctacgttat gagctacagt aaatccactg
      841 gaaccttcat caagataaat agggcatgtc caataatctg accatttaga tgcagaaata
      901 tcgtgagagt aattgtacat tatctcagaa cccgtgcctt gatttgaaag agtgtaaata
      961 gcaccgccat cagacaaaag ttgtgctacg tgatgaatct cattcctatt aattttatta
     1021 ttagtcatcg ctgtttgttg tttagtccat ccaaaaccaa cagagatacc cgtataattc
     1081 atataagaga cctcattatg ctctatcacg atataacgag gatatccagc aatgatacca
     1141 actccccctt gaatttctgt agtgccgtga gtcactaaat tgtttcttat cgtatcacgt
     1201 gtgctaattt catctttatc agctggatta tagggaatat gaatttctgt agtgctatca
     1261 gctgcaaatt ttccaattga aattccagag cccccgatat ctgtaaatat attgccttca
     1321 attctattat catttgttcc agaaataaaa tctaagcctg tcgccgccat ttgtgtaaag
     1381 acgttgcgtt caaaaataaa gtgactcaca ttcgttacag aaacaccggc attagggcgc
     1441 catagtaaaa acttattact tccaagaagt ccattttctt caagtacttc gacattgaat
     1501 tggcccccct gcaaattcaa aaagccttct ttacttggtc ttaaaaagtt tgagtttgca
     1561 aaaatcaaac ctttaaattt catattttgc actttattct ttgtgctttc ccctttgatt
     1621 tctactaagg tctctaaatt cggcgccacc acagtagcac tagttaaaga ttctccagag
     1681 cgtggcttgt agtaaagaat atctgtcgtc tcatcaagat accactctcc aggttgatct
     1741 aaaaattcat aagcattttc aaaataaaaa gtctgttgtt ttggaggatt actcataaaa
     1801 gcagtgccaa gcataggata ctttcttcta aaaagtttag acctttcagg atcctgcgga
     1861 atcactttgg atacactacc atttctttca attttttcta agcggagaac agattctgcc
     1921 catgcaatca tcaaatgtat ctccacttta ttcatattct tccaatcccc tgtatattca
     1981 gaagaaacat caaaagcacg ccccatagaa tcgactttcg tcaatctaaa catgttatga
     2041 gaaccatctg aatttaaatt tgggtgacgt gcacggattc cttttttacc attaacataa
     2101 agttgtctaa aacgagcatc aacgccctca gcacaccaaa tatcatttgc ttcatcacat
     2161 aaagaccaag acggtactgg tataccacct gtgataaacg gcttttctcc tgtgtagttt
     2221 tcataacgca cataaaagcc attcgttcca ccatccgaat tattaaagcg aagagtattg
     2281 tctaatacat aagtaccatc acgcaaataa acaacaatat cgcccgtcat agacgaatta
     2341 atcgcccgaa cagcttgctg tgctttttga atcgtcttaa aaggcgcttc ttttgttcca
     2401 gcattagcgt cacttcccga aggagccaca taaaaaacag cttgctctgc cgcaaataaa
     2461 ttagcaacgt atgtcaaaat gatgcccatg atgagcacga tttttttttt catcctaatc
     2521 tccctaagcc ctttttttta cttaatcact cgataagcat ggtgactatc cctaatcaca
     2581 tacttaccta ccgggaatct ttcatgaatt tctcttttac aatcctgaat tgaattcgat
     2641 tgaatatcac ctaaatactg accttgtaaa ctaaagactt tataagtttg aagcccttgt
     2701 ttttctaaat caaaagtctt tgaaatggaa ttcgtttttt ctccaaactc aatccaatca
     2761 atattcacat aactacctgt gatcaataat ttgagaactt gctcgccaga agaaatggct
     2821 gaagtcgttc cttcaatttc agcataagta tcccaatcac cagtcgtttc tattttgatc
     2881 gtatcagtga ttgctttatc gcccaagaat aattggaaac tagaactttc tgatccacta
     2941 gaaactcttg ctctaaaagg ataagctttt gcttcttcta cattgactgt atattcaagc
     3001 cactcgccag cttctgtata tccaatcata taatttccaa attcatcagc aacgatatca
     3061 actctatctt cacggtattt ggctccctca ttttctattg atacgtcata ataagcttta
     3121 ttcgatccgc caacatcata attttctact tctatttttc ctggtattgg agttgcaaca
     3181 caacccgtct tatctacacc acagaatggc ccttgaggaa tagccgctgc tgccgtttca
     3241 aaataccagt agtccaattc aaacatgctg gttccacctt tgaatacaaa gaacaagtca
     3301 tgaacgccaa cagctcctga aacatcaaca ctctgttcca cgaaatcgtt cgcagacgtt
     3361 acatttaact ggccaatcat ctcaccatct tgcttgtcta agcgaatttc gatggtaccg
     3421 cttccgcctt tcactgcggc gtagaattta tctgcaccat catcgccaaa atctacagaa
     3481 cgaaccttga tatagtcatt gttgtccaac ttattcacgt atacgccctt gccttctaca
     3541 cggtaggtgc caatgcccct agaaaaagcc tttgtttcag cctgaaccac tttaaatgga
     3601 tctaagttgt gaattggttt tactactccc aaatcagtag gtttaaccaa aggaatagta
     3661 ccatcagaat tgaacgtgaa ttcatctact tgagtagaac gactaaagcc gccaccacca
     3721 ggaagttttg cggtgtggta gaatataaac gcacgtccct tgaattcaat gatacctgca
     3781 tggcttgtaa aagcaaagcc actatgttga tctagaatga cacctttcca ttcccaaggc
     3841 ccagtagggc tatcgctcca cgaataagaa attttctcag gaacaccatg agaagcataa
     3901 atcatataat atttatcccc acgcttgtga atccatgggc cttctgtgta cttggaggat
     3961 tccccgttag gagcaaagcc acgactcatg tccgttacgt tgatttgccc atcaatggtg
     4021 atcatgtctt cttttagctt ggcatagtaa agcttgggat taccccaata aatataggcc
     4081 tgatcatcat cgtcaacaaa tacggtaggg tcaatgtagt cccagtttgg gccagccata
     4141 tgcctgttgt aagcttcttt aaagggacca tctggtttgt cagcaacgcc tacagcaata
     4201 gcgcggccac ccttagtgga ttccacagtc acatagtaat agaacttgcc gttacgataa
     4261 atgcattcag cagcccagtc gccagcaggc ttcgcatttg taatactttc atggccaaaa
     4321 acgaacccac ggtcagtcca attcaccata tcagtactag aatacacgcg ccaattattc
     4381 atggtaaaga aggatcctcc ttcatcatta cctgtataca caaacacggt atcattccac
     4441 accataggcg cagggtctgg agtgtagtat gcctgagcaa taggattatc tgccatgcct
     4501 aacgaaggca ataaaagaac agctagatat cctaaaagtg tagttctaaa attctgtttc
     4561 ataatatccc gtcccgttat tttaattcaa taagttgatt tgttttttct gtttttgaac
     4621 gaactaaata aacgc
//
LOCUS       DAOOBB010000160         4530 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_21557_length_4530_cov_5.068827, whole genome shotgun sequence.
ACCESSION   DAOOBB010000160 DAOOBB010000000
VERSION     DAOOBB010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21557_length_4530_cov_5.068827"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PLT31_07360"
     CDS             complement(<1..319)
                     /locus_tag="PLT31_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPW94988.1"
                     /translation="MLLEKSFSHLKTGSQYAYKEHAYLVLQMLNQRIYDDLAIEEQRS
                     YSEYRFIRAVPVIGGEEITLSKLAEYPIRSHYAGIIGYFQLEPDGALKTPVLPDGALE
                     SIPV"
     gene            complement(376..1089)
                     /locus_tag="PLT31_07365"
     CDS             complement(376..1089)
                     /locus_tag="PLT31_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPW94989.1"
                     /translation="MNEQNSNVTILIVEDEMAIAEGLIDICELNGYKVKHVIDGETAL
                     VEALSGKYGLVLLDLMLPGLDGFTVCDRIREKDKDLPIIILSAKNSDNDIIDGLKYGA
                     DDYIPKPFSVPMLLARIEAVLRRSRHSLEGDGKLVAGNLRIHFREYMGTRGNEELVFT
                     RKEIEILEYLWNNRDHAVPRAELLKKVWGYENAESVDTRTVDIHITKLRKKIEDDPSH
                     PKLLVTFRGEGYQFRASPE"
     gene            complement(1140..1940)
                     /locus_tag="PLT31_07370"
     CDS             complement(1140..1940)
                     /locus_tag="PLT31_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94990.1"
                     /translation="MRIQTMLAAFALCATQSFAIFGIGGHYSPSVTTKLNASKQQTVK
                     SIEVENATVGQVLYAHKSFSSMQGFGFKAWIDILPIIDIEATYNIQWGSYDASLFIQA
                     GNEDPIEQPLEVEFGGVPFGKATPKYVGMNGDLSVTYPITFLPIVRPYVGAGITYFLN
                     TPLMDSSFIAKFMNSADNVAGNILLDQNSQMDASKGAELAKALAKEVAEEELQKSIGA
                     HILLGVRIDPPIIPIAFYVNGKYYIGGDFHDDIEMGRFVFEVGAGFAL"
     gene            complement(2219..3523)
                     /locus_tag="PLT31_07375"
     CDS             complement(2219..3523)
                     /locus_tag="PLT31_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HPW94991.1"
                     /translation="MDNSSAPAPLAERLRPKSLEDFLGQNKIMGPKSLLRKSLENDCV
                     PSMIFWGPPGCGKTTLAHIIKGKSQKNFICISAISSGVKEVKSLLAEAREPEARKKGT
                     ILFIDEIHRFNKGQQDALLGAVEDGTITLIGATTENPGFEVNKALLSRCQLILFAPLS
                     EDDLKNLLDSALKTHPKGLLRTDISIAPEVVEKLVHQSDGDARYLLNQIEWIVGNLGD
                     KKELDLTVLDEIQYKKPLRYDKSGEEHYNLISVFHKSIRGSDPDAALYWLHRMLQGGE
                     DPRFILRRLMRIAMEDIGLADPNALLLATSAREAFDFMGVPEGLIALDELAVYLSLAP
                     KSNSLEIAGMTADQIIQRTGMLPVPKAFRNPVTRVGRELGYGEKYEYDHDSEGAYSGQ
                     DHLPSSLNGTVIYRPTLYGKEKQLAERLQSLNAIKKKKKDSL"
     gene            complement(3526..3939)
                     /locus_tag="PLT31_07380"
     CDS             complement(3526..3939)
                     /locus_tag="PLT31_07380"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPW94992.1"
                     /translation="MLFSTIKHIAKIEVRYAETDAMGVVHHSVYPIWFEQSRTEIMRI
                     NDCSYHSLEAEGFSCPVLQMELEYKHSCHYGEFAEVHVSIAKEDRLRTRFFYEIYVNN
                     QLCVKGSTLHIFLKDGKPSRQGPESFEKIFFPKAP"
     gene            4182..4254
                     /locus_tag="PLT31_07385"
     tRNA            4182..4254
                     /locus_tag="PLT31_07385"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4215..4217,aa:Thr,seq:tgt)
     gene            4261..4342
                     /locus_tag="PLT31_07390"
     tRNA            4261..4342
                     /locus_tag="PLT31_07390"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4295..4297,aa:Tyr,seq:gta)
     gene            4385..4456
                     /locus_tag="PLT31_07395"
     tRNA            4385..4456
                     /locus_tag="PLT31_07395"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4417..4419,aa:Thr,seq:ggt)
BASE COUNT         1402 a          843 c          860 g         1425 t
ORIGIN      
        1 caacagggat ggattcaaga gctccatcag gaagcacagg ggtttttaaa gctccatctg
       61 gctctaactg aaaataccca atgatgccag catagtggct ccgaatggga tattcggcta
      121 gtttagataa agtaatttct tcgcctccaa tcacaggaac tgcacgaata aaacgatatt
      181 cagagtaaga cctttgttct tctattgcta aatcatcgta aattctctga tttaacattt
      241 gtagaactaa ataggcatgt tctttatagg cgtactgaga accagtttta agatgagaaa
      301 acgacttttc aagtagcatt atcacaggaa atgctattgc tataaatatt aatataaatg
      361 tgattcttat gttcttcatt ctggagaggc gcgaaactga tagccttcgc ctcggaacgt
      421 cacaagaagt ttaggatgag aagggtcgtc ttcaattttt ttacgtaatt tcgtaatatg
      481 aatatcaacg gtacgagtgt ctactgattc cgcattttca tatccccaaa ctttttttag
      541 aagctcggct ctaggaacag catgatctct attgttccat aagtattcga gaatttcgat
      601 ttcctttctc gtaaagacaa gttcttcgtt tcctcgagta cccatgtatt ctcggaaatg
      661 aatgcgtaaa ttgccagcga ctaattttcc atcgccttct aaagaatggc ggcttcttct
      721 gagaacggct tctattcttg ctaaaagcat tggaacagaa aaaggttttg ggatatagtc
      781 atcagcacca tactttaaac cgtcaatgat atcgttatca gaattctttg ctgaaagaat
      841 aatgattgga agatctttat ctttttctcg aattcggtca cagactgtaa agccatctaa
      901 tccaggaagc ataagatcta atagaactaa tccatatttt ccagaaagag cttctacaag
      961 agcggtttcg ccatcaatga catgtttcac cttatagccg tttagttcac aaatgtctat
     1021 aagcccttca gcaatagcca tttcatcttc tacaataaga atagttacat tagaattttg
     1081 ttcattcata ttactctcaa aaccagttta atgtgatgcc caatcccatc atttatttat
     1141 tataatgcaa agcctgcacc gacttcaaaa acaaatcttc ccatttctat atcgtcatgg
     1201 aaatcgccac caatgtaata tttcccattt acatagaatg caatagggat aatgggaggg
     1261 tcaatacgaa cgccaaggag gatgtgagcg ccaatgcttt tttgtaattc ttcttctgcc
     1321 acttctttag cgagagcttt ggcaagttca gcacctttag aagcatccat ttgagaattc
     1381 tggtcaagta gaatatttcc agcgacatta tcagcagaat tcatgaattt agctatgaag
     1441 gagctatcca ttagaggggt gtttagaaaa taggtaatgc cagcaccaac gtatggacga
     1501 acgatgggta agaaagtaat aggataagtc acagataaat cgccgttcat cccaacatat
     1561 tttggagtgg cttttccaaa aggaacgcca ccgaattcaa cttcaagtgg ttgctcaata
     1621 ggatcttcat ttccagcttg aataaacaag ctagcgtcat aagaacccca ttggatattg
     1681 taagttgctt cgatgtcaat aataggaaga atatctatcc aagccttaaa gccaaaacct
     1741 tgcatggaag aaaaagattt atgagcatat aaaacttgac caacggtagc attttctact
     1801 tcaatagatt ttacagtttg ttgtttagat gcatttaatt ttgtggtgac gctaggggaa
     1861 taatggccgc caataccaaa tatcgcaaaa gactgagtcg cacataaggc gaatgcagca
     1921 agcatggttt gaatacgcat aaaggtctcc ttgtggggtt ttcttgaaat taattaaaat
     1981 ttatcgaaaa agatattaaa atataaagag aaaccttaat aaaatgtatg tgtagtctag
     2041 tttgataggt ttatatggat tgaaaatgaa atgaggatgt tcaaaaaaga atatgaaaat
     2101 ggctaatatg ctcaataatc gaccatttta agtcgaaaaa gcggttgata agaatacatg
     2161 tgaatcccga aaaaaggtat tctatttata taaattaaaa tgaattttta aaatttctct
     2221 ataaagaatc ttttttcttc ttttttattg catttaaaga ttggagcctt tctgctagct
     2281 gtttttcttt tccgtataaa gtgggtctgt atattacggt tccatttaag ctacttggta
     2341 aatgatcttg ccctgaataa gccccttcac tatcatggtc atattcatat ttttctccat
     2401 aaccaagttc tcttccaact cgagtcactg gattacgaaa agcttttggt acgggaagca
     2461 ttcctgtgcg ttgtataatt tgatctgctg tcattccagc aatttcgaga ctattgcttt
     2521 ttggagctag agataaataa acagctaatt cgtctaatgc aataagccct tcaggaacac
     2581 ccataaagtc aaacgcttct ctagcggagg ttgcaagtaa taatgcattg ggatcagcaa
     2641 ggccaatgtc ttccatggct attctcatta gacgtctgag aataaagcgc gggtcttctc
     2701 ctccttgaag cattcggtgc agccagtata gtgcggcgtc tggatcgctt cctcggatgg
     2761 atttatgaaa aacagaaatt aaattgtagt gctcttcgcc agatttatcg tatcgtaatg
     2821 gctttttgta ctgaatctca tctaaaacgg ttaagtctaa ttcttttttg tcacctaaat
     2881 ttccaacaat ccactcaatt tggtttaata aatagcgagc atctccatcg gactgatgta
     2941 ctagtttttc aaccacttca ggggcaatag aaatatccgt tcttaataga cctttgggat
     3001 gagtttttaa ggcagagtca agaagatttt taagatcgtc ttcagagaga ggggcaaata
     3061 aaatgagttg gcatcttgat aaaagagctt tatttacttc aaaaccagga ttttctgtag
     3121 tagctccaat tagagtgatt gtaccgtctt ctacggctcc aagtagagcg tcttgttgtc
     3181 ctttattaaa gcgatgaatt tcatcaataa aaaggatagt accttttttt cttgcttcag
     3241 gttcacgagc ctcagcaaga agagatttta cttctttcac accgctagaa atagcagaaa
     3301 tacagataaa gtttttttga cttttccctt taataatatg ggctagagtc gttttaccac
     3361 acccaggagg cccccaaaaa atcatgcttg gaacacaatc gttttcaaga ctttttctta
     3421 aaagactttt aggccccatt attttatttt gccctaaaaa atcttctaaa gatttaggac
     3481 gtaggcgttc tgctaagggg gctggagcgg aagaattatc catacttagg gagcttttgg
     3541 aaaaaagatt ttttcaaagg attcgggccc ttgtcgactt ggtttaccgt ctttaagaaa
     3601 gatatgaaga gttgatcctt ttacacacaa ttgattgttt acataaattt cataaaagaa
     3661 tcgagtccga agacggtctt ccttagcaat gctaacatga acctcggcaa attcaccata
     3721 gtgacaggag tgtttgtact ccagttccat ttgaagtaca gggcatgaaa agccttcagc
     3781 ttcaagtgaa tgataagaac aatcatttat tcgcataatc tcagttctag attgttcaaa
     3841 ccagataggg tagacagaat gatgtacgac tcccatggca tctgtttctg cataacggac
     3901 ttcaattttt gcgatatgtt tgattgtact aaataacatt tatgacttcc cgaaaaataa
     3961 taaataggct gttttataaa gatagaaaaa agcaaaataa aagtattaac attctatcaa
     4021 cagtgtatat acaagaaatt ctaatttttg ggcctttttt tctcaaaaaa aaactttttt
     4081 ttcataactc tttgattttc attgacaaaa ttgatgaatc ctttctacat ttggcacgct
     4141 ctaaagttat acatgttaag tgttataatt ttcttgtaga tgccaaagta gctcagctgg
     4201 tagagcagct gatttgtaat cagctggtcg taggttcgat tcctatcttt ggctcttaaa
     4261 gggtaggtgc ccgagtggtt aaaggggacg gactgtaaat ccgttgacta tgtctacagt
     4321 ggttcgaaac cactcctacc catgaacccc aatcatcctg tcacggttgt ggcaggatga
     4381 ttttgcccag gtagctcagg ggtagagcac ttccttggta aggaagaggt ctcgggttca
     4441 attcccgatc tgggctctca ctaattcgga ggcttaaaat ggcaaaagaa aagtttgata
     4501 gaagcaagcc gcactgcaac atcggcacca
//
LOCUS       DAOOBB010000161         4513 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_21668_length_4513_cov_3.803724, whole genome shotgun sequence.
ACCESSION   DAOOBB010000161 DAOOBB010000000
VERSION     DAOOBB010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21668_length_4513_cov_3.803724"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="PLT31_07400"
     CDS             <1..1053
                     /locus_tag="PLT31_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94993.1"
                     /translation="EVVLLKNLVASASSKTNLGKIKLQKAAYVQSLFKYDTTGASINL
                     GSHFEIHVEGSSYQTSVLSGESFVLGMGANVKALVVFPADVFMIQKLIDAGISHTDIY
                     QRVEVDIVEGDTLKLDSIVWKFPVQEPVFIDSTLGRIKGRVIGLDGKPQAWANVRLIE
                     DIYGFGFAMGQGLFNNLNTVTDTLGWFELPFPSAVEDSFRIEVDFYEYGNLIATGVSE
                     YLHKDELTNKSDTITTADIVLQKPSSFMGFITLISDSSDPYSSNCVLNSIVLGFKGTS
                     HFVRQITCYPIQMNALPTGSQELIFYTGDYNVIQVLRQENEPIDTYMQMLTVNLPEGD
                     TLSQQGITYTPPTKTK"
     gene            1096..1166
                     /locus_tag="PLT31_07405"
     tRNA            1096..1166
                     /locus_tag="PLT31_07405"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1128..1130,aa:Gln,seq:ctg)
     gene            1220..1993
                     /locus_tag="PLT31_07410"
     CDS             1220..1993
                     /locus_tag="PLT31_07410"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HPW94994.1"
                     /translation="MNSDFLDFYSKHYGDRWPLLLEALFKETHPVELRFSDSLQSYFL
                     DEASLFAAKCLDVQPGMAVLDMCAAPGGKSLVLASQLAGQGSLQCNDRSPERRIRLQH
                     VLENTLPAAWHSIIKVTGYDGSRFGLHCKGQFDRILLDAPCSSERHVIQSPKHLEQWS
                     LNRIKRLSVEQGSLLASAVDALALGGVLVYSTCALSPFENDAVVEKILKKRKEKIRVM
                     NISPSIDGSDSTSFGVHILPDRSEGRGPIYCAKLEKISD"
     gene            complement(1999..2163)
                     /locus_tag="PLT31_07415"
     CDS             complement(1999..2163)
                     /locus_tag="PLT31_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HPW94995.1"
                     /translation="MKKYRCTVCGYIYDEADGDPDSGVAPGTLFEDIPDDWVCPVCGV
                     TKSDFELAED"
     gene            2263..3663
                     /locus_tag="PLT31_07420"
     CDS             2263..3663
                     /locus_tag="PLT31_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94996.1"
                     /translation="MKRFCLWLALLSVFSFAREVPVRYLTMDDSLEREAIFVKLVKDT
                     VYLREYTPEEIAAKQAKALAEAEEQAIIEKYGDIEDSSSVNSTTEEASESVEPSEEEA
                     TVPEEAVSQEPAADDFEAALLVTDSLKEAKADSSATDSLKVDSLKTDSVQAVEAPKDV
                     YIRLFKYDFKRLINVESGEMVDLTLSDYEYTEEEEEEEYIPLYPEGKANLLVKSIPEA
                     CSLFVNGVPLEMLTPDTIKNIKPGKYNISIKKRLKDVDWWGNKAVKINDDSLNVIEVE
                     VERPSTRLTVNTVPEAVEVYLDESPNENVMPHYMSDVVIEDIKPSTEMMVYFRKVGYL
                     DTAIITEVQAFMPNTILVELEPVTMNLGIIDMQKEYNRERTSRFIGRGLLWSSIVPFV
                     SAGVLWYLAEQDWKEAKDKKDAYESGSAFYNEDTRKLVEDNRKYNDRGDVKGFTALGL
                     GILGVTLLSVGFVLSF"
     gene            complement(3779..4207)
                     /locus_tag="PLT31_07425"
     CDS             complement(3779..4207)
                     /locus_tag="PLT31_07425"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94997.1"
                     /translation="MKIPQISIFVSNRPGRLHAVCKSLADADINLLSLTLADSGEFGL
                     IRIIVSNPEKAVEVLSESGIVATVTDVIACEVSALKGGLAKLLSTPAGSLQIDYMYAY
                     PTCNQKEIAIMILRFRDPDEAISLLEKAGVQLLSRKELLV"
     gene            complement(4204..>4513)
                     /locus_tag="PLT31_07430"
     CDS             complement(4204..>4513)
                     /locus_tag="PLT31_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HPW94998.1"
                     /translation="VNVFPSQIETALLRAEKALPHYQIVLDRKNHLDTVEVKVEVSRD
                     MMSDSMSDMDALNKRFSHSIEQILGISVLVTLCEPGSLPRSEGKAKRVIDNRTQGGSL
                     "
BASE COUNT         1319 a          775 c          971 g         1448 t
ORIGIN      
        1 gaagtggtgc ttcttaaaaa cttagttgca tcggcttcaa gtaaaacgaa tttaggtaaa
       61 ataaaacttc aaaaagcggc ttatgtgcaa agtctcttta agtatgatac gactggagca
      121 tctattaact taggctctca ttttgagata catgtagaag gctcttcata tcagactagt
      181 gttctttctg gtgaatcctt tgttcttggt atgggtgcga atgtgaaggc attagttgtt
      241 ttccctgcag atgttttcat gatccaaaaa ttaattgatg ctggaatttc tcatacagat
      301 atttatcaac gagttgaagt ggatatcgta gaaggggata ccttaaaatt agattctatc
      361 gtttggaaat tccctgtcca agagcctgtt tttatagact ctactttagg tcgtataaaa
      421 ggacgtgtga tcgggttaga tggtaaacct caagcatggg ctaatgtacg tttaatagaa
      481 gatatttatg gttttggttt tgcgatgggc caagggttgt ttaataattt gaatacggtg
      541 acagatactc ttggttggtt tgaattaccc ttcccttcag cggtagaaga tagttttcgt
      601 attgaagtgg atttttatga gtatggtaat ctaattgcca ctggagtttc agaatacttg
      661 cataaagatg aattgactaa taaaagtgat accattacaa ccgctgatat tgtattgcaa
      721 aaaccatcct cttttatggg ttttattacc ttgatctctg attcctcgga tccatattct
      781 tctaattgtg ttttgaatag tattgtactt gggtttaagg gaacaagtca ttttgtgcgc
      841 caaattactt gctatccaat acaaatgaat gctttgccta cgggttctca agaacttatt
      901 ttctatactg gagattataa cgtaattcag gtattaaggc aagaaaatga acccatagat
      961 acctacatgc aaatgctaac tgtgaacctc ccagaagggg atacgctttc tcagcagggg
     1021 atcacctata ccccccctac caaaactaaa taacccttgc cgacgttcaa ttattttata
     1081 aatttgactt cgcactgagc tatggtgtaa tggtagcaca acagattctg aatctgtttg
     1141 tcttggttcg agtccaggta gctcaatctt tttgtgccga atgcttttta taaaggcttc
     1201 tttttaccgg atcagggtga tgaattcaga ttttttagac ttttatagta agcattatgg
     1261 tgatcgttgg cctttattat tagaggctct ttttaaagaa actcatcccg ttgaactacg
     1321 cttttctgat tctttacagt catatttttt agatgaggcg tctttatttg ctgcaaaatg
     1381 tttggatgta caaccaggga tggctgtact cgatatgtgt gctgctcctg gtggaaaatc
     1441 tttagttctt gcttctcaac ttgctggtca aggatcatta caatgtaacg atcgttctcc
     1501 agaaaggcgt attcgtttgc aacatgttct tgaaaacacg ctccctgcag catggcactc
     1561 tattattaaa gtaactggtt atgatggctc tcgttttggg cttcattgca aaggccagtt
     1621 tgatcggatt ttattggacg ctccatgttc ttcagaacgt catgtgattc aatctccaaa
     1681 gcatttagaa cagtggtctt tgaatcgaat aaagagatta tcggtggagc aaggctcctt
     1741 acttgcctca gcggtagatg cacttgcgct agggggtgtt ttagtttaca gcacttgcgc
     1801 tctttcccct tttgaaaatg atgccgttgt tgaaaagatt ttaaaaaaaa gaaaggagaa
     1861 aattcgcgtg atgaatattt ctccttcgat tgatggctct gatagtactt cttttggagt
     1921 acatatttta ccagatcgct ctgaagggcg aggcccaatt tattgtgcaa aactggaaaa
     1981 aatcagtgat tagtttttct agtcttcagc gagttcaaaa tcggattttg tgaccccgca
     2041 cacagggcaa acccaatcat ctggaatatc ttcaaaaaga gtgcctggag ccacaccaga
     2101 atctggatct ccgtctgctt catcgtagat atagccacaa acagtacaac ggtatttttt
     2161 cataatttct ccactggatt aaagttttaa tcaatatacg ctttttttgt tgattgagta
     2221 ttcgattttt caatttggac ttttttatgt tattttttga atatgaaacg tttttgtcta
     2281 tggcttgccc tactttctgt tttttcattt gctcgtgaag tacccgtccg ttatttaacg
     2341 atggatgact cattagagcg tgaagcaatc tttgttaagt tagtgaaaga tacggtatat
     2401 ttaagagaat atacgccaga agaaattgct gcaaaacaag caaaagcact tgctgaagca
     2461 gaagaacagg ctattattga aaaatatggc gatatagagg attcttcttc tgtgaattca
     2521 actacagagg aagcttctga atcagtagaa ccttcggaag aagaagcgac tgtaccagag
     2581 gaagcagtct cgcaagaacc tgctgctgat gattttgaag cagctctctt agtgaccgac
     2641 tcgctaaagg aggcgaaggc agattcctcc gcaacagatt ccttaaaagt ggattctcta
     2701 aagacagatt ctgtgcaagc ggttgaagct cctaaggacg tttacattcg tttgttcaag
     2761 tacgatttta agagattaat taatgtggaa tcaggtgaga tggtcgatct tacgctatct
     2821 gactatgaat atactgaaga agaagaagaa gaagaatata ttcctcttta tcctgaagga
     2881 aaagccaatc ttcttgtgaa gtctattcca gaggcttgta gtctttttgt aaatggtgtt
     2941 cctcttgaga tgttaacccc tgatacgatt aaaaatatta agcctgggaa gtataatatc
     3001 tcaatcaaga aacgcttaaa ggatgtagac tggtggggta ataaagcggt caaaataaat
     3061 gatgattcct taaatgtgat tgaagtagaa gtggaacgtc ctagtactcg gttgacggtg
     3121 aatactgttc ctgaagcggt agaagtttat ttagatgagt ctccaaatga gaatgtgatg
     3181 cctcattata tgtcggatgt ggtgattgaa gatataaaac ctagtactga aatgatggtt
     3241 tattttagaa aggtggggta tttggatacg gcaattatta ctgaagtgca agcttttatg
     3301 ccaaatacaa ttctagtcga gttagagcca gtgacaatga atttgggtat tattgatatg
     3361 caaaaagaat ataatcgcga aaggacatcg cgctttatag gtcgaggttt attatggagt
     3421 tcaattgttc cttttgtgag tgctggtgtt ttgtggtatt tagcagaaca agattggaaa
     3481 gaggcaaaag acaaaaagga tgcttatgag agtggatctg ctttttataa tgaagatact
     3541 cgaaaactag tcgaagacaa tagaaagtat aatgatcgag gtgatgtaaa agggtttaca
     3601 gctctgggcc ttggtatctt aggtgtgact cttttatctg taggatttgt tttatcgttc
     3661 taaattaaaa caagtgaaac ctaaggaacc gccgtgtacc gaacggtacg cacggtggtg
     3721 tgagaggacg gtaggtaaaa taattaccta cctcctactt gattataaaa gaatagtgtt
     3781 ataccaagag ctcttttcga ctgagtaact ggacacccgc tttttctaaa agagagatcg
     3841 cttcatcagg gtctctaaag cgcaaaatca taatggcgat ttccttttga ttacaggtgg
     3901 ggtaagcata catataatca atttgtaaag aacctgctgg cgtactgagt aacttggcaa
     3961 ggccgccttt taaagctgaa acttcacatg caatcacatc ggtgactgta gcgacaattc
     4021 ccgattctga taggacctct actgcttttt ctggatttga aacgatgatt cgaattaatc
     4081 cgaattcacc gctatctgca agagttaaag ataaaagatt gatgtccgcg tctgctaaag
     4141 atttacaaac ggcatgtaaa cgccctggac ggttagaaac gaaaatagaa atttgtggga
     4201 tcttcataag gaacctcctt gtgtgcgatt atcaatgact cgtttggctt tgccttcact
     4261 tctagggagt gaacctggtt cacagagagt gaccaataca gaaataccaa gaatttgttc
     4321 tatggaatgg ctgaaacgtt tatttaatgc atccatatca ctcatgctat cactcatcat
     4381 gtctcttgaa acttccactt tgacttctac tgtatccaga tgattcttgc gatcaagaac
     4441 aatctggtaa tgaggaagag ctttttcagc tcgaagaaga gccgtttcaa tttgtgaagg
     4501 gaatacattg acg
//
LOCUS       DAOOBB010000162         4471 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_21905_length_4471_cov_5.345335, whole genome shotgun sequence.
ACCESSION   DAOOBB010000162 DAOOBB010000000
VERSION     DAOOBB010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21905_length_4471_cov_5.345335"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="PLT31_07435"
     CDS             complement(<1..401)
                     /locus_tag="PLT31_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW94999.1"
                     /translation="MSHLRSILMPVAIILGILIPQAYVLAFLLPYMIGIMMVLTFASR
                     VPRQEHGETFKIVLKSSLMSFFLAIVLFAVYRLLDLPIEIFLSGLIIILAPPANAAPA
                     MAKVLGGNSILALKIFISGNVIACFSIPLIFG"
     gene            464..1864
                     /locus_tag="PLT31_07440"
     CDS             464..1864
                     /locus_tag="PLT31_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HPW95000.1"
                     /translation="MLRSRYEEMPLSQIKVSKKKRWNAIQVVILGHLSLIFLGTLLLS
                     LPFAKAEPIRFLDSFFTSCSAVCVTGLSVLDIGTAYTHQGHWIILFLMEIGGLGIMTI
                     STTLILLAGMRPGFNQQSVLLSTFTTDNDVDASKILKAIIPFTLLLQLFGVLIYFTQF
                     KELPTYDRFFSSLFHAVSSFCNVGLSLFPDSLMRFQTNTVVNITTCILVIAGGFGFLA
                     ITEMQHFFNFKTREFQRLSLHTKIAAICTLILIIGGGLILWFTESSTTIKDLSFADKM
                     LSTFFISISARTAGLNTIDMATLSLNSIFLIIIMMIIGSNPGSCGGGIKTTTAAVIAL
                     LGFNRLLGRQKTQVLGRTIPEETVDRAVRIFVIAIVVLVGATLILLHTEAATATMAGS
                     ESSLFIKILFEVASAYSTCGLSLGLTPELSDPGRIIICIVMFVGRMGPLFLISAVARH
                     DSESGMWFAEEDIMVG"
     gene            1888..2544
                     /locus_tag="PLT31_07445"
     CDS             1888..2544
                     /locus_tag="PLT31_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HPW95001.1"
                     /translation="MSSKQFVVIGLGNSGYFLARHLTALGHDVLVIDSSPEKIQDITA
                     SVSQAVVADSTRKKQMASLPLNKVDSVVVCIGEDLEASLLTVLNLKELGVKHIIAKSS
                     SPAHTVILEKLGVSDIFHPERDMAISLAERMNRPNMLDYLPFMEGFSIIELACPEKFI
                     GKTLKDLSLTKKYGVQVIAIRDPLETEPKLGNLADYVLQERDVLFIIGPNKVLDKIKD
                     "
     assembly_gap    2692..2791
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2792..3350)
                     /locus_tag="PLT31_07450"
     CDS             complement(<2792..3350)
                     /locus_tag="PLT31_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPW95002.1"
                     /translation="MKVWVCSVCGYVHMGEEPPEVCPVCKAPASKFNLKEQSKKEKTW
                     ICAVCGYKHVGEEALDECPQCKAPKSKFKLQEMSLTEKIIWADEHKIGVAQGLDEWVV
                     QSLRENFTAECTEVGMYLAMSRQADRDGLPEVAEAYKRIAFEEAEHAAKFAELLGEVV
                     HADTKKNLELRVAAEAGATEGKLELA"
     gene            complement(3454..4359)
                     /locus_tag="PLT31_07455"
     CDS             complement(3454..4359)
                     /locus_tag="PLT31_07455"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HPW95003.1"
                     /translation="MTRFNDYNESSTAKKNISIILLVLSVVYTLFPNIPFPFDDIALI
                     IAAISNAGYHLLNPQQIIIKRVMNVLRKILFAIYIVIVVFGGLILLITLHLAGVDFKN
                     ALWIYVFILATLLFMGRFFQNKLVGWIGEVYSASVIRMITRGVVLKDVYIEGSSNVVQ
                     IDIIAITEKGVLVIEKKTMIGLILGDEYQKQWTISLKRGKLKYPMKNPLHQNYGHIKV
                     LEEKFPEYKDFFINLVVFGNNAKLGNKIPDNVITDRQFLKYYRSLPNRMSPSLQEDFT
                     SLLMELSKNREEVKRKHKNKIRQYK"
BASE COUNT         1312 a          856 c          740 g         1463 t
ORIGIN      
        1 ccaaaaatga ggggaattga aaagcaagca atgacattgc ctgatataaa tattttcaat
       61 gctaaaatgg agtttcctcc aagtaccttt gccatcgctg gagcggcatt ggctggagga
      121 gctaaaatga taatcaaacc tgaaaggaaa atttctatgg gtaaatccaa aaggcgatag
      181 actgcgaaaa gtactatcgc caaaaaaaag ctcattaaag atgatttaag aacgattttg
      241 aacgtttctc catgctcttg acgaggcact cgagacgcaa acgttagcac catcataata
      301 ccaatcatat aaggcaataa aaaagctaaa acataggctt gaggtatcaa aatacctaga
      361 ataattgcca caggcatcaa aatagaacgt aaatgactca cgttacaaat gtagtttata
      421 atcctttttt aggcacccct ctttttttta gtaaattttc gctatgttac gatctcgtta
      481 cgaagaaatg cccttatctc aaattaaggt gagcaaaaag aaacgctgga atgccattca
      541 ggtggtgatt ctcggccatc tctctttaat ttttttgggg actcttctat tatcgctccc
      601 ctttgcaaaa gcagagccta ttcgttttct agattcattc tttacttctt gttctgctgt
      661 ttgtgttaca gggctttctg ttttagacat aggcacggct tacacacacc aggggcattg
      721 gattattcta tttcttatgg aaatcggtgg gcttggtatt atgactattt ctaccacatt
      781 gattttactt gcagggatga ggcctggatt taatcaacaa tccgtattac tatccacctt
      841 caccactgat aacgatgtgg atgcttctaa aattctaaaa gccattattc cttttacgct
      901 cttacttcaa ttatttggcg tccttatcta ttttacgcaa ttcaaagaat tacccaccta
      961 tgatcgtttt ttctcttctc tgttccatgc ggtcagttct ttttgtaatg taggtttatc
     1021 tctttttcct gattctctaa tgcgttttca aaccaatacc gttgtgaaca taaccacatg
     1081 tattcttgtc attgcaggtg gttttggttt tttagccatc actgaaatgc aacatttctt
     1141 caatttcaaa acaagagaat ttcaacgtct ttctcttcat acaaaaattg ccgccatttg
     1201 cactcttatt ctcattattg gtggtggatt aattctttgg tttacagaat catccactac
     1261 aatcaaagac ctttcttttg cagataaaat gctttcgaca tttttcattt ctatctctgc
     1321 tcgaactgct ggtttaaata ccattgatat ggcaacgctt tccttaaact ccattttcct
     1381 aatcattatc atgatgatta taggttctaa tcctggcagc tgcggcggtg gaatcaaaac
     1441 aacaacagct gctgtgattg cattacttgg atttaatcgt cttttagggc gtcaaaaaac
     1501 acaagtttta gggcgtacta ttcctgaaga aaccgtggat cgagccgttc gaatttttgt
     1561 gattgctatt gtggtgcttg taggagcaac gcttattctt ctacatacag aagcagccac
     1621 agctacaatg gctgggagcg aaagttccct ttttatcaaa attttatttg aagtggcaag
     1681 tgcctacagc acctgtggac tttctttagg gcttacccca gaactttcag accctggccg
     1741 tattataatt tgtatagtaa tgtttgttgg ccgtatggga cctttatttt taatttctgc
     1801 tgtggctcgc catgattctg aatctggaat gtggtttgca gaagaagata taatggtggg
     1861 ttagattttt aaaaaaggat tacagctatg tcatctaaac aatttgttgt cattggtctt
     1921 ggaaactccg gctatttttt agctcgccat ttaacagctc ttgggcatga tgttcttgta
     1981 atagactcaa gtccagaaaa aatacaagac atcaccgcct ctgtttctca agcggttgtt
     2041 gctgatagca caagaaaaaa acaaatggcc tctctccctt taaataaggt cgatagcgtg
     2101 gtggtttgca ttggagaaga cttagaagcc tctctactca cagtcttaaa ccttaaagaa
     2161 ttaggcgtaa agcatattat cgcaaaaagc tcaagccctg cacatacagt gattcttgaa
     2221 aagctaggcg tttcggatat tttccaccct gaacgcgata tggctatttc tcttgccgag
     2281 cgcatgaatc gccccaatat gcttgactat cttcctttca tggaagggtt ctctattatt
     2341 gaactcgctt gcccagaaaa atttattggg aaaacactaa aagacctctc tctaaccaaa
     2401 aaatatggcg tgcaagtgat tgccattaga gatcctttgg aaaccgaacc aaagcttgga
     2461 aacttggccg actatgtatt acaagaacga gatgttcttt tcatcatcgg accaaacaaa
     2521 gtattagata aaattaaaga ttaatgcgtc gcgagttagc gtttcgtgga atttatgatt
     2581 ttacgaataa tcttttagtt aacatttcgc gagtttaatg tttttgaata agcgtttcgt
     2641 gatttcttga ttaacgtttc gcgggtttat gattctcaac ttttttaatt gnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcgccaatt caagctttcc ttctgtagcg
     2821 cctgcttctg cagccacgcg aagttccaag tttttctttg tatcggcatg aaccacttca
     2881 cctaaaagct ctgcaaactt agcggcgtgt tctgcttctt caaaagcaat gcgtttgtaa
     2941 gcttctgcta cttctggaag gccatctcga tcggcttgac ggctcatcgc caaatacata
     3001 ccgacttctg tacattccgc agtaaagttt tcacgaagag attgaacgac ccattcatct
     3061 aatccttgcg ccacaccaat tttatgttca tcagcccaaa ttattttttc agtgagactc
     3121 atttcttgaa gcttaaactt agattttgga gccttacatt gagggcattc atccaaagcc
     3181 tcttcaccaa catgcttgta accacaaaca gcacaaatcc atgtcttttc ttttttagac
     3241 tgttctttaa gattaaactt tgaagctggt gccttacaaa caggacaaac ttcaggaggt
     3301 tcttcaccca tatgaacata accacaaacg gaacaaaccc atactttcat attaactcct
     3361 atgcgctaaa cgcattttgt tttaagttta attttgtaat gattacaaat ataaaatgat
     3421 tataataatt tggcaaatgg aaaataaggg agactattta tattgtctaa ttttgttttt
     3481 atgctttctt tttacttctt cgcgattttt acttaattcc attaaaagtg aagtaaaatc
     3541 ttcctgtaga gatggactca ttctatttgg aagcgagcga taatacttta aaaactgccg
     3601 atctgtaata acattatctg gaattttgtt tcctaatttt gcattattac caaaaacaac
     3661 tagattaata aaaaaatctt tatattctgg aaacttttct tccaatactt ttatgtgacc
     3721 ataattctga tgtaaaggat ttttcattgg atattttaat tttcctcttt tcaatgaaat
     3781 agtccattgt ttttgatact catcgcctag aattaatcca atcattgttt ttttctcaat
     3841 caccagaaca cctttctccg taatcgctat aatatcaatt tgtacaacat tacttgagcc
     3901 ttcaatatag acgtctttaa ggacaacgcc tctagtaatc attcgaataa cacttgctga
     3961 atatacctct ccaatccagc cgactaactt attttgaaaa aaacgcccca taaataatag
     4021 cgttgctaat ataaaaacat aaatccacaa agcattttta aaatcaactc cagctaaatg
     4081 gagtgtaata agaagaataa gcccgccaaa aacaacaatc actatataaa tcgcaaataa
     4141 aattttccgt aaaacattca tcaccctttt tataattatt tgttgcgggt ttaacaaatg
     4201 atatccagcg ttagaaatag cagcaataat caaggctatg tcgtcaaaag ggaaaggaat
     4261 gtttggaaaa agagtataga ctacacttag cactaaaaga ataattgaaa tatttttttt
     4321 tgctgtagaa gattcattat agtcattaaa acgagtcatt tctattcaat ataatttata
     4381 ttttcgatga gatatgcaat gtcgttatta gattctttat ttggaaatcg tttaggagct
     4441 gttttcgtca aagaaaattg tgatgcaagt g
//
LOCUS       DAOOBB010000163         4439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_22082_length_4439_cov_3.795849, whole genome shotgun sequence.
ACCESSION   DAOOBB010000163 DAOOBB010000000
VERSION     DAOOBB010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22082_length_4439_cov_3.795849"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="PLT31_07460"
     CDS             complement(<1..393)
                     /locus_tag="PLT31_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPW95004.1"
                     /translation="MAERIISPEQNINEDLDQEQNLRPSSLQDFTGQSILKENLSIAI
                     EAAKHRNDSLDHCLFAGPPGLGKTTLASIIAKEMGVHIHITSGPVLEKASDLAGLLTN
                     LQENDILFIDEIHRLNRVIEEYLYPAMED"
     gene            complement(397..990)
                     /gene="ruvA"
                     /locus_tag="PLT31_07465"
     CDS             complement(397..990)
                     /gene="ruvA"
                     /locus_tag="PLT31_07465"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HPW95005.1"
                     /translation="MIEQLRGIMAQKHPTFVILDCGGVGYGIHVSSRTGDTLPEIGTE
                     VTLLTHLVVREDAMILFGFSQIQEKELFLQMIEVNGIGPKMAQRILSSVSVNDFLSMI
                     AREDKVALGKIKGIGKKTSEMLVLALKDKALSISALSGDTTPEILLPNAMQEAIAALH
                     TLGVKDPAAKKAVEKAASILGEAANVSLLIPEALKHV"
     gene            complement(1121..1915)
                     /locus_tag="PLT31_07470"
     CDS             complement(1121..1915)
                     /locus_tag="PLT31_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95006.1"
                     /translation="MMLAGCAATTKGGLPDHIDFDNVESAEVAMHAQEFRTMDSLSEI
                     GWMLAGQDGVAWIASDSLMADLPKDKRVGHSGWVAQGNLRDGFCIFYGKDSSGLFQIA
                     RYDFSSGHMSRSVARTELPEGKIYDLVQKNESAYNFFKNSGEAYKIPYNTYILENEGQ
                     YTFYVIPGSTSEYAILGGAFKLTSVDTTWQMEKLHKSPTIMKWAAIKGNMLVRTSSMG
                     DLLNEADFAQYYIYEKSLPEQYIKTQKYLIALLHDEKGKMTIMVVK"
     gene            2410..4323
                     /locus_tag="PLT31_07475"
     CDS             2410..4323
                     /locus_tag="PLT31_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 44 protein"
                     /protein_id="HPW95007.1"
                     /translation="MKHLNSMLLCGLSFLGSSVLCGTAFADISIAVDASVGVQKISPY
                     LYGRNIDVISDGEDSDAALLEKENDFYSKMLESGMYFLRANNGNNATRYNWRKKMTVH
                     PDWYNNVYEHDWGVTAQKVIDNMPGVDVMYAFQLTGYAASTTEHNFSDWAFYQEHGMW
                     ATGTLDLAGGGEASEDGTTLIKAGDYSLYNETWPADSTVGIIDHWRDELKLDMSRFQY
                     WSMDNEMEIWSGTHGDLPLDIDADFLVERYIDVAKKARNKWKDIKLTGPVVANEWQWC
                     SVGATGGKSGLGKGEDRDYCWLEYFIMRLAAEQKASGVKMLDVFDMHWYPSEKSYEDR
                     VNWHRVFFDTTYNYPGANGIKLVSGSWDNNQKKEYIFKRVNDWLDKYFGENHGITLGI
                     TETSIIDESDPMTTALTYASWLGTFMDHGVEIFTPWTWGDGMYETVHLFSRYGQDYRV
                     ASTSSNDSLLSAYTSVSAAKDSMTVILVNRAENNAENVTIDVAGLKNMKPAAITMTLA
                     GITGETFESHAVNALKAGEAQVTGTKVSLTVPAKSITTVILSADAVAGIAPASVAANL
                     LSSQGNAWFVNNSAGNIRSVQIFTSLGQEVRNMNELSRGNVSLQTEKLVQGNYIIRIH
                     STSGSQIQKISVK"
BASE COUNT         1223 a          913 c          985 g         1318 t
ORIGIN      
        1 atcttccata gcggggtaga ggtattcttc aatgactcga tttaaacgat gaatttcatc
       61 aataaataaa atatcgtttt cttgaagatt agttaaaagc cctgctagat cacttgcttt
      121 ttcaagaacg ggtccactag taatgtgaat gtgtacaccc atttcttttg caataatact
      181 tgctaaagtt gtttttccaa gccctggagg ccctgcaaac aaacagtgat ctagagaatc
      241 atttcgatgt tttgcagctt caatggcaat acttaaattt tcttttaaaa tagattgacc
      301 agtaaaatct tgaagtgaag aaggacgaag attttgttct tgatccaagt cttcattaat
      361 gttttgttct ggggatataa tacgttcggc cattttttat acgtgtttaa gtgcttctgg
      421 aataagtaga gagacatttg ctgcttcacc aagaatactt gccgcttttt caacagcttt
      481 tttagcggct gggtctttta cgcctaatgt atgaagagct gctatcgctt cttgcattgc
      541 attagggaga agaatctctg gagtggtatc tccactaagc gcagagatcg acagtgcttt
      601 atcttttaat gcaagcacaa gcatttcaga tgtttttttg ccaatccctt taattttacc
      661 aagtgctact ttatcttctc tggcgatcat gcttagaaaa tcgtttacag aaacactgct
      721 caaaatgcgt tgagccattt taggcccaat gccatttact tcgatcattt ggagaaagag
      781 ttctttttct tgaatttgac taaaaccaaa taaaatcatg gcgtcttctc ggactaccag
      841 atgcgtgagt agggtgactt ctgttcctat ttcaggaaga gtatctccag ttctagaaga
      901 aacatgaatt ccataaccaa caccaccaca gtctaaaatg acaaaagtag ggtgcttttg
      961 agccatgata ccacgaagtt gttcgatcat cgaaggtcct cgagaaagtg ttactgctca
     1021 aatatatact atattaagag ataatgtcaa gctaaattgt ctgctatggt catttttttc
     1081 aaaacagcgg ttttctagct ggtttcttta atttttgtcc ttactttaca accattatag
     1141 tcatcttgcc tttttcatca tgaagaagag caattaaata cttttgggtt ttgatatatt
     1201 gctctggcaa gctcttttcg tagatgtaat actgagcaaa atcagcttca ttcagcaagt
     1261 ctcccataga ggaagtacga acaagcatat ttcctttgat tgcagcccat ttcatgatgg
     1321 ttggactttt gtgaagtttt tccatttgcc aagtagtatc tacggaagtc aacttaaagg
     1381 ctccgcccaa aatagcgtat tcgcttgtag aaccaggtat gacataaaac gtatactggc
     1441 cctcattttc taggatgtat gtgttgtacg gaattttgta ggcttcgcca gaatttttaa
     1501 agaagttgta cgcagactca tttttctgca ccaagtcata gatcttaccc tctggtaatt
     1561 ctgttctggc aacagagcgg ctcatatgtc ccgaagaaaa atcatagcgg gctatttgaa
     1621 aaagacctga agaatccttc ccgtaaaaga tacagaaacc gtctctcaag ttgccttggg
     1681 caacccatcc ggagtgtccg actcgtttat ctttaggcaa gtccgccatc aaagaatcag
     1741 aggcaatcca agccacacca tcctggccag ctaacatcca tccaatttca ctgagagagt
     1801 ccatggttct gaactcttgg gcatgcatag ccacttcagc agattccacg ttatcgaaat
     1861 ctatgtgatc tggcaatcca cctttagtcg tggctgcaca tccagcaagc atcattagca
     1921 acgagaaacc aactatcaca aaagtttttt ttgtcatctt cctaaccttt ttaagttttt
     1981 ttaatgtaat taaaaaataa acgattataa ccagattcta acacagagcg ttcctgcggt
     2041 cttgtgttta gaccttcgca gccatttagc cctaatcact tgagaactta aaatagttta
     2101 tagtctcatt cagagctgga ttggggaaag tcgattgcat tagcttgctg aggtgttact
     2161 ggtgaagatt ggttgtggat ttgataatat cgtcatggaa agtttttttg tgcaataaaa
     2221 caggaaacag gttttggtaa aatgtagccc ttttactttg aaacaattaa aaaagcggca
     2281 tcactgtcca tgattttgta gtaagtcaat gtgtaattct caacgccttt tgaaaagaag
     2341 gcgcgttttg tttttagttc cttataaatc tgtttatttt gttagaacag gttataaaag
     2401 taggtggtta tgaaacactt gaattctatg ctcctttgcg ggctctcttt tttaggaagt
     2461 tctgttctgt gcggtaccgc ttttgcagat atttccattg ccgtggacgc ttctgtcggc
     2521 gttcagaaaa tttcacctta cctctatggt cgtaatatcg acgtcattag cgatggtgag
     2581 gattctgacg cagctcttct cgaaaaggag aacgattttt acagcaaaat gctcgaatca
     2641 ggcatgtatt ttttgcgtgc caataacggc aataatgcca cgcgatacaa ctggcgcaaa
     2701 aaaatgacgg tgcatccaga ctggtacaat aacgtttatg aacacgactg gggcgttacc
     2761 gcacaaaagg tcattgacaa catgcctggc gtagatgtca tgtacgcttt ccagttgacg
     2821 ggctatgcgg cctccacaac ggaacacaat ttttcggact gggcttttta tcaggaacat
     2881 gggatgtggg caactgggac gcttgacctt gcgggtggcg gcgaagcttc tgaggatgga
     2941 acaacgctta taaaggcggg ggactacagt ctgtataacg aaacgtggcc agctgattct
     3001 actgttggca ttattgatca ttggcgagat gaactgaaac tagacatgtc tcgtttccag
     3061 tattggagca tggataacga gatggaaatc tggtcgggaa ctcatggaga cttgccgctg
     3121 gatatcgatg ccgattttct cgtagagcgt tatattgacg tggcgaaaaa ggctcgaaac
     3181 aaatggaagg atatcaaact gactggacct gttgtagcca atgaatggca gtggtgctcc
     3241 gtgggggcaa caggtggaaa gtctggacta ggtaagggcg aagatcgcga ttactgctgg
     3301 ctcgaatatt ttatcatgcg tcttgccgcc gaacagaagg cgagtggcgt gaagatgctc
     3361 gacgtgtttg atatgcattg gtatccttct gaaaagtcgt acgaagatcg tgtaaactgg
     3421 caccgtgttt tctttgacac cacctacaat tatcctggtg caaatggaat caagctggtg
     3481 agcggttctt gggacaataa tcagaaaaag gaatacatct ttaagcgcgt aaacgattgg
     3541 ctagacaagt atttcggtga aaatcatggc attactctgg gtattaccga aaccagcatc
     3601 attgatgaat cagacccgat gacgacggcg cttacctacg cctcctggct tggaacattc
     3661 atggaccatg gcgttgaaat ctttactccg tggacatggg gcgacggcat gtacgaaaca
     3721 gtccacctgt ttagccgtta cggccaagac tatcgcgtgg cttcgacctc cagtaacgac
     3781 tcgttgttgt ctgcctacac ttccgtttct gcggctaagg attccatgac ggtgattcta
     3841 gtgaaccgcg ctgaaaataa tgctgagaat gtaacgattg atgttgctgg tctcaagaac
     3901 atgaagcctg cggcaatcac catgaccctt gctggcatta cgggagagac ctttgaatcc
     3961 catgcggtga atgcactaaa ggcgggcgag gctcaggtaa caggaaccaa ggtgtcgctt
     4021 acggtcccag cgaagtccat tacgacggtg atcctttctg ccgatgcggt tgcaggaatt
     4081 gcccctgcgt cggtggcggc aaacttgctt tcttctcagg ggaacgcttg gtttgtgaac
     4141 aattctgcag gaaatatccg ctcggtgcag attttcacta gcctcggtca agaggtgcgt
     4201 aatatgaacg aattatcccg tgggaatgtt tcgcttcaaa cggaaaaact ggttcaagga
     4261 aactacatta tccgcatcca ttccacaagc ggctcgcaaa tccaaaaaat ctccgtaaaa
     4321 tagcgaatca ttttattcgc tttcgcaaag ggtggattgc tccttttaat caggtcgagt
     4381 caagtgactg gcctgttttt ctatataacg cacaaaatat ttgaaaattg aaaccgacg
//
LOCUS       DAOOBB010000164         4438 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_22086_length_4438_cov_4.762948, whole genome shotgun sequence.
ACCESSION   DAOOBB010000164 DAOOBB010000000
VERSION     DAOOBB010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22086_length_4438_cov_4.762948"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="PLT31_07480"
     CDS             <1..885
                     /locus_tag="PLT31_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95008.1"
                     /translation="NVGWWYTEVDCYNPFEDNHDGQCPDGSILQKRVANSDNGSKEGY
                     VDYTMELLNTQKYYDVLGRKANRNSKQKQALYRLDKMNSQNSKLTEDTELSDKIRRLL
                     NKVFSSKKVLQKAETCGTLGGDYGIVELDGSLVGGITCPFLSVKPIDIKESSSTKKGV
                     CNDGSAIIDVFVEITFRSFLTSNDYFVVPVNYVFPENGHRTTEEDVRKLKKHELGHVA
                     YNKCIEAKFSNEVVVESKQFCECGTSLEKSFEKWRDQLYENKYSHDRTRFEKARDLFH
                     EKYGQFDGADTYVCPSNL"
     gene            827..1492
                     /locus_tag="PLT31_07485"
     CDS             827..1492
                     /locus_tag="PLT31_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95009.1"
                     /translation="MKNMDNLMELIPMSVQVISKSFVYLLLFAGISFAQRDSSLALED
                     TITAKYCLHADTRINFSKNNENIWILSVYEQIIPFTFTIQTRLYKFSLIGKAELISGI
                     SDLKSEKKIIKGFRCGGSSSANKKEYRCIMGQKDQYYIYDDLFFSYDFSDSTKIRALF
                     GKTLPLEWNLNFSIDRQKQTLSGTHYVYISGFCNKEQMAQIREINKEREKAMQKPKVR
                     WIK"
     gene            1648..2364
                     /locus_tag="PLT31_07490"
     CDS             1648..2364
                     /locus_tag="PLT31_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95010.1"
                     /translation="MQEETCSIVLMENTAMPQVMIVLIKSIFFYCCFCTGILFATEDK
                     AVYCINKEEPETLVLVRNIEQLFVFPDTVIGTESCEMISIFAGVTITEKESILRTYMT
                     IGGRGMATNSNIHLYKKTEEEKPVLLPPKKLSNEQYLGFPKSFQEKQTIHSLFPNQEN
                     SFIVPTKWSFTIKCKSGDEFEMVKSADILISGYCTKEELKPYLEEKTKMQNTSNTLNS
                     SNCVEPIHSNEFKEDSSLGR"
     gene            complement(2487..4286)
                     /locus_tag="PLT31_07495"
     CDS             complement(2487..4286)
                     /locus_tag="PLT31_07495"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPW95011.1"
                     /translation="MKKTFIIGNRMQKKLQVLFFSLMAFSTAFAGFENCTFNFGRQWK
                     SGASLSNLDHVAIWLGDNNDYNTYWEGEMIRTAKSNNVTPVIYAYVIAEYGKDNGLVD
                     CDMGSPNHCTGGANMIRNHWSSIISRYSNYAQGIASDYGTDKAIIWLIEPDFVQYSVT
                     GDAKSSWNQDGGGIPDANLAGKYFNEIVSTIKGKLPNAKIAVDISPWFNNDLATWYNL
                     FDKSKIDYAFTSGGRTQGDNARIRGDNNNNVTWANVKSWLGKSIIADDGYGVGGGSNS
                     DYQEWMNTSNLNARIADGVIGLTIQEPVDSYYTFAKSNASLNTCSGQSSSSVKSSSSS
                     VPVSSSSVTPSSSSVKSSSSSIPVSSSSIIPSSSSVSSSSSSATPSSSSVSSSSSSVV
                     ESAEWTAENSNLSNEKVNGVSIGQGSDWGKTRTVSRTLATVNSGTAYTLSFEASVSRG
                     GASMDVEVALNEYCSEPLHVSSSGDVAKYTCTFTASKTESVTLQFTVPGERWETLTIT
                     NLQLVGPSGDILPIRTIASLGQIQSSVLLRNGRTIHWSITPRTTLQVFDVNGNKISQT
                     RGSFLDLSKLPAGLYIIRSNNGNHSEFKRVNNF"
BASE COUNT         1505 a          712 c          854 g         1367 t
ORIGIN      
        1 aacgtgggtt ggtggtacac agaagtggat tgttataatc cttttgaaga taatcatgat
       61 ggacaatgcc cagatggttc catactgcaa aaaagagtgg caaattcaga taatggctca
      121 aaagaaggct atgttgatta cactatggaa ttattgaaca ctcaaaaata ctatgatgta
      181 cttggacgaa aagcaaatcg taattcaaaa cagaaacagg cattgtatag gttagataaa
      241 atgaatagtc aaaactccaa attgacagaa gatactgaat tatctgataa aatccgacgc
      301 ttgcttaata aagttttttc gagtaaaaaa gttttacaaa aggctgaaac ttgtggcaca
      361 ttaggtggtg attatggtat tgtagagttg gatggctctt tagtgggagg aataacttgt
      421 ccttttctgt ctgtgaagcc aatcgacatc aaagaaagtt ctagtacgaa aaaaggagta
      481 tgtaatgacg gctctgccat cattgatgtt tttgttgaaa ttacttttcg atctttttta
      541 acttctaatg actattttgt tgttcctgta aattacgttt ttccggagaa tggtcataga
      601 actacagagg aagatgtaag aaaattaaaa aagcatgaac tcggccatgt tgcttataat
      661 aagtgtatag aagcaaaatt ttcaaatgaa gtagttgttg aatcaaaaca gttctgtgaa
      721 tgtggaacta gtcttgaaaa gagttttgaa aaatggagag atcaacttta tgaaaataag
      781 tatagtcacg atcgaactcg atttgaaaaa gcgagagatc ttttccatga aaaatatgga
      841 caatttgatg gagctgatac ctatgtctgt ccaagtaatc tctaaatcat ttgtttatct
      901 gttgcttttt gcagggatct cttttgctca aagagattct tcgcttgctc ttgaagatac
      961 tataacggca aaatattgct tgcatgctga tacaagaata aatttttcaa aaaataatga
     1021 aaatatttgg attttatctg tttatgagca gataattcct tttacattta cgattcaaac
     1081 aagactttat aaattctcat tgataggaaa agccgaatta atttcaggta taagtgattt
     1141 gaaatccgaa aaaaaaatta taaaaggatt tagatgtggt ggaagcagta gtgccaacaa
     1201 aaaagaatat cgttgtataa tgggtcaaaa agatcaatac tatatttatg atgatttatt
     1261 tttctcttat gacttctctg attcaacaaa aatacgagca ctttttggta agacgctccc
     1321 actagaatgg aacctaaatt tctcaattga tcgccaaaag caaactttat caggaactca
     1381 ctatgtttac atttctggct tttgtaataa agaacaaatg gctcaaatta gagaaataaa
     1441 taaagaacgt gaaaaagcga tgcaaaagcc aaaagtaaga tggataaaat aaaaatgaag
     1501 tagttgttga atcaaaacag ttctgtgaat gtggaactag tcttgaaaag agttttgaaa
     1561 aatggagaga ttaactttat gaaaaaaaat atagtcgcga tcggattcga tttgaaaaag
     1621 tgttaaactc attaataaat attattgatg caagaagaga cttgttccat cgtacttatg
     1681 gagaatacgg ctatgccaca agttatgatt gtcctaatta aaagcatttt cttctactgc
     1741 tgtttctgta caggcatttt atttgcaaca gaagataaag cagtatattg tattaataaa
     1801 gaagagcccg aaacccttgt tttagtccgt aatattgagc aattatttgt atttccagat
     1861 acagtaatcg gtacagagtc ttgtgaaatg atatctatat tcgcaggcgt gactataact
     1921 gaaaaagaaa gtattttgcg tacttatatg accataggtg gaagaggtat ggcaaccaat
     1981 agtaacattc atctttataa aaaaactgaa gaagaaaaac ctgtattgtt accacctaaa
     2041 aaattatcta atgaacaata tttaggtttt cctaagagtt ttcaagaaaa acaaacaatt
     2101 cactctttat ttccaaatca agaaaattca tttattgtac caacaaaatg gagtttcact
     2161 attaaatgta aatcagggga cgaatttgaa atggtaaaat cagcagatat attgatttct
     2221 ggttattgta caaaagaaga gttaaaacct tatttggaag aaaagactaa aatgcaaaat
     2281 acttcaaata ctttaaactc gtcaaattgc gttgaaccaa ttcattcaaa tgaatttaag
     2341 gaagacagtt ctcttggtag gtgaatatca taaaaaacgc accatcgttt aatggagcca
     2401 attgtttttt tacaagcatt aaaaaaatcc cttgtgtaac acaagggatt ttgcattaga
     2461 tgcttttctg aattagtatt tgtgaattag aaattattca ctctcttgaa ttcggagtgg
     2521 tttccgttat tggaacggat aatataaagg cctgctggta gtttggatag atctaagaac
     2581 gagcctctag tttgtgatat tttgttgcca ttgacatcaa atacttgaag cgtggttctt
     2641 ggtgttatgc tccaatgaat ggtgcggcca ttacgtaata gtacagaaga ttggatctgc
     2701 cctaaagaag cgatggtgcg aataggtaga atgtcgccac tggggcctac caactgtagg
     2761 tttgtaatgg ttagagtttc ccaacgctcg cctggaacag taaattgtag tgtaacagat
     2821 tctgtttttg aggcggtaaa agtgcatgta tatttggcaa catctccaga acttgaaaca
     2881 tgaagaggtt ctgaacaata ttcgtttaat gctacttcta catccatcga agctccgcct
     2941 cttgatacac tggcttcaaa agaaagtgtg taggctgtgc cagaattgac tgtagcaaga
     3001 gttctcgaaa cagttcttgt tttaccccag tcagagcctt gcccaatcga gactccattc
     3061 actttttcat tggataggtt tgaattttct gcagtccatt cagcagattc tacaactgaa
     3121 gaagaacttg aagagactga agaagagcta ggtgttgctg aagaagaact tgaagagact
     3181 gaagaagaac ttggaattat tgaggaagag ctcacaggaa tagaagaaga gctagacttc
     3241 acggaagaag aacttggagt aactgaagaa gagctcacag gaacagaaga ggaacttgac
     3301 ttcacagaag acgatgattg tccgctgcaa gtattcaagc tggcattgga ttttgcaaaa
     3361 gtgtaatagg aatcaacagg ttcttgaatg gttaatccta taacgccatc tgcaattcta
     3421 gcatttaggt ttgaagtgtt catccattct tgatagtccg aattactccc tccaccaact
     3481 ccataaccat catcggcaat tatacttttg cctaaccatg atttaacatt agcccatgtg
     3541 acattgttat tgttgtctcc tctgattcga gcattgtctc cttgagtgcg acctccagaa
     3601 gtaaaggcat agtcaatttt acttttatca aataaattat accatgtggc cagatcatta
     3661 ttaaaccaag gggaaatatc tacagctatt ttagcatttg gtagttttcc tttaatggta
     3721 gaaacgattt cattaaaata ttttccagca agattggcat ctggaatgcc tccgccatct
     3781 tggttccagg aggattttgc atctcccgtg acggagtatt ggacaaagtc aggttcaatc
     3841 aaccagatga tggctttatc agttccataa tcggaggcga ttccttgtgc gtaattggag
     3901 taacgtgaaa taatagaact ccaatggtta cgaatcatat tcgctccacc agtacaatgg
     3961 ttgggagaac ccatgtcgca atctactaat ccattgtctt tgccgtactc ggcaatcaca
     4021 taggcataga ttactggggt gacgttattc gatttggctg ttcgaatcat ttcgccttcc
     4081 caataagtat tataatcgtt gttgtcaccc aaccatatag ccacatggtc taagttggat
     4141 aaagaagcgc cgcttttcca ttgacggcca aaattaaatg tgcaattttc aaagcctgca
     4201 aaagcagttg aaaaagccat taacgagaag aaaagaactt gtaatttttt ttgcatacga
     4261 ttcccaatga taaatgtttt tttcaaacac caccctaacg actaatgcat aattaacagt
     4321 agtcgtatcc cacactaata tccatctcta atttacagtt ttatagcata aatgcttgta
     4381 aaaaagacat ttacagagat tttttagtgt cgtttttatg tgacctaaaa caagttga
//
LOCUS       DAOOBB010000165         4436 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_22098_length_4436_cov_4.507875, whole genome shotgun sequence.
ACCESSION   DAOOBB010000165 DAOOBB010000000
VERSION     DAOOBB010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22098_length_4436_cov_4.507875"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PLT31_07500"
     CDS             complement(<1..320)
                     /locus_tag="PLT31_07500"
                     /inference="COORDINATES: protein motif:HMM:NF023583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HPW95012.1"
                     /translation="MTSIAQRQELQAKIWKIANDVRGSVDGWDFKQFVLGTLFYRFIS
                     ENFSAHFEAGDESLVYADLKDSVITPEIKEDAIKTKGYFIYPSQLFENVTKNANDNAN
                     LNTD"
     gene            complement(510..1364)
                     /locus_tag="PLT31_07505"
     CDS             complement(510..1364)
                     /locus_tag="PLT31_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95013.1"
                     /translation="MMKKIFMLTLSASLLSIIACGEKKGTIEGVVLDPFTGKAVEMPT
                     VWMDSTIFSTQKSKYEFKGELQKGQFKFVNVPVGDYLIKARRNRYVLGQEKFTTSDKS
                     PDAKLTLYIYNDQVDPGLYKPNPEGAEKISNNWVLWSASCKESVAAYRLSYVDSKEMP
                     SLPNDKKSKKKKAAPAGKEMPLPSARIVDSNIDFLYKNASSVTAPLMAASYAVETDNV
                     ASHTDCQGFNGDKKGLFANESKKTDLEVSYKSEALYQVKGTLPKGKQVLVLSQDGKTL
                     QTYYFEVK"
     gene            complement(1445..1744)
                     /locus_tag="PLT31_07510"
     CDS             complement(1445..1744)
                     /locus_tag="PLT31_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HPW95014.1"
                     /translation="MKEGIHPNYKPTVFVDANTGKEYITRSTKSSSEKKTIDGVEYNV
                     ISLEITADTHPFWTGKQHRVDTAGRIDRFNKRFSSNIVGAKRKTRKAVSAEESAE"
     gene            1836..2387
                     /locus_tag="PLT31_07515"
     CDS             1836..2387
                     /locus_tag="PLT31_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95015.1"
                     /translation="MQIVLITLFLLMFGCSTENNNYEPVITGKPYVLNEWPDSLYVQS
                     LDSIIKAEPVKKDADSSEKTEISLNSFQMPKVISAPTKNEKPTPTATTSANTTKKVDP
                     FPNRFMQALEKWQSDPSNPSLYVSVIALENENLFQLMGRFYKTNTNVLPKFYTTSALQ
                     SVNPGVSVEKIKAGDSIRLPRLK"
     gene            complement(2408..2992)
                     /gene="hisB"
                     /locus_tag="PLT31_07520"
     CDS             complement(2408..2992)
                     /gene="hisB"
                     /locus_tag="PLT31_07520"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HPW95016.1"
                     /translation="MRCSKISRKTNETQIDLSLNLDKASRGKIDSGSAFLDHMLDLFQ
                     VHGGFTLDLVCKGDVAVDMHHSIEDIGIVLGQALVECLGDKKGIERYGFYFVPMDEAL
                     ARVCIDFSNRIGFVWNVKLNSSSVGTMDASLFEHFFKSIAENARMNLHVELFYGSDNH
                     HCLEAIFKAFARAIAMAKSPSRQIDGVPSSKGVL"
     gene            complement(2994..3986)
                     /locus_tag="PLT31_07525"
     CDS             complement(2994..3986)
                     /locus_tag="PLT31_07525"
                     /inference="COORDINATES: protein motif:HMM:NF024805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPW95017.1"
                     /translation="MPVNDKVFESKNENESLAAYLTRARVARGYTIEDMVRETRISIT
                     YMKNIEAGDWSKFPVDAYLRGYLNSISRILELDYKEVLSSYLKEKGSPDYAEQQVVEK
                     ETAVSSEREKKSFLMPIIVVVLGLAFFVLMNFVNQFEKTEESVSNNPLMKESSVAPQD
                     SEESVESSTLIHDGAELVVLDSLVRDSLVKDSLSSDSIQAVVGTTAVDTISKKKELPA
                     SATIFISSSSKEKVQVNTKNTSFVLIGSGVANSWVGLKRNESDTAFLKEANISREGGK
                     LVYETSDTLYVIIGDPVAIAKMTLNGKETALPKMKNGLVTRFRVFNGQIIPGVR"
     gene            complement(3977..>4436)
                     /locus_tag="PLT31_07530"
     CDS             complement(3977..>4436)
                     /locus_tag="PLT31_07530"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HPW95018.1"
                     /translation="SPMQWPLLYAEMKAKDVSKSHPNSIVLGCDTLVFRDGVPLGKPK
                     TPEDAYFLLQSLNGRSHQVRSGIAFAYGGEVIKSKIEETEVFFRNNDPQDLLEYVNSL
                     EPMDKAGAYGIQGRGASLVKSIQGCYYNVVGLPVATTLDLLKEVEKFLCP"
BASE COUNT         1391 a          914 c          738 g         1393 t
ORIGIN      
        1 aaatcggtgt ttaggttagc gttatcattg gcgtttttcg ttacattctc gaatagctgg
       61 ctgggataaa tgaaataccc cttcgttttt atggcgtctt ctttgatttc aggggtaata
      121 acggagtctt tcaggtccgc atacacaaga ctttcatccc ccgcctcaaa gtgtgcggag
      181 aagttctcgc tgataaatcg ataaaataat gtacccaaga caaactgctt aaaatcccac
      241 ccatcgaccg atccacggac atcattggct attttccaaa ttttagcttg caattcttgc
      301 ctttgtgcta tgcttgtcat tatgtttcct ttattgttag caaaccactt ttaggactat
      361 taaaagtata gcaaaacaat ctctcacagc atgagcgatc accttcttta atatccatat
      421 tttttacgct ttcacaaaga tattttctat ccatcaaaaa agtccagcaa ccggggtagt
      481 tactggactc tttacgtatt aaaacaaaat tacttgactt cgaagtaata agtctgaagg
      541 gttttaccat cttgagaaag cactaaaact tgctttcctt ttggtaaggt tcctttcact
      601 tgatataggg cttcggactt gtaagaaact tctaaatcag tctttttaga ttcgtttgca
      661 aagagacctt tcttgtctcc attaaagcct tggcaatcgg tatgagaagc tacattgtcc
      721 gtttcaacag cataagaagc tgccatcaga ggagccgtaa ctgagctagc attcttgtat
      781 aagaaatcaa tattagaatc aactatgcga gcgcttggta atggcatttc tttaccagca
      841 ggagcagctt tcttcttctt tgatttctta tcgttaggca aacttggcat ttctttagaa
      901 tccacataac ttaaacgata agccgctaca gattctttac aagaagcaga ccaaagtacc
      961 cagttatttg aaatcttttc ggcaccttct ggatttggtt tgtaaagacc tggatccact
     1021 tgatcgttat aaatataaag agttaattta gcatccggac ttttatctga tgtcgtaaat
     1081 ttttcttgac ctaaaacata acgattgcgg cgtgctttta tcaaatagtc accaacagga
     1141 acgttaacaa atttaaattg gcctttttgt aattcaccct taaactcata cttagatttt
     1201 tgagtagaga aaatggttga atccatccat accgtaggca tttcaacagc tttacctgta
     1261 aacggatcaa gaacaacgcc ttcgattgtt ccttttttct ctccacaagc aataatggaa
     1321 aggagagagg cgcttaatgt aagcataaat atttttttca tcatatttgc accttttata
     1381 gaggttataa ctataaaaaa acctctataa cgaggttttt tttaagctca attaatcaaa
     1441 acaattactc agcagattct tcagcagaaa cggctttgcg tgttttgcgt tttgcgccga
     1501 cgatattgct ggagaaacgc ttgttaaagc gatcgatacg accagcagta tcaacacggt
     1561 gttgtttacc tgtccagaat ggatgggtat cagctgtaat ttctaaagaa atcacattgt
     1621 attcaacacc atcaatagtc ttcttttctg aagaagattt tgtagagcga gtgatgtatt
     1681 ccttaccagt atttgcatca acaaaaacgg taggtttata attagggtga atgccttctt
     1741 tcataatatt taatcctgct aaaaacagcg tacgaaaagc cgaaatttaa gatttttgag
     1801 cttttttggc aaggctataa atgtattttt aaaccatgca aatagtctta atcacacttt
     1861 ttctcttaat gtttggttgt tccactgaaa acaacaatta tgaacccgta attacaggga
     1921 aaccctacgt tttaaacgag tggcccgata gcctttacgt tcaatcttta gactctatta
     1981 tcaaggcaga acctgtcaaa aaagacgctg atagttcaga aaaaacggag atttccttaa
     2041 attcattcca aatgccaaaa gtaatctcgg caccaactaa aaacgaaaag ccaaccccta
     2101 ctgcaacaac aagcgctaat acaacaaaaa aagtagaccc atttcccaat cgttttatgc
     2161 aggctttaga aaaatggcaa tcggaccctt ctaatccttc attatatgtc agtgtaatcg
     2221 ctttagaaaa tgaaaatcta tttcaattaa tgggtcgttt ctataaaaca aatacaaatg
     2281 tacttcctaa gttctacact acatctgcac tacaatctgt gaatcctggt gtttctgtag
     2341 aaaaaataaa agccggagat tcgatccggc tacctaggtt gaaataaaac cgcttaatta
     2401 aaacggttta taaaacgcct tttgaacttg gtacgccatc aatttgacgt gatggtgatt
     2461 tagccatcgc gatagctcta gcaaatgctt tgaatatagc ttctaagcaa tgatgattat
     2521 cactgccata aaataattct acatgcaaat tcattcttgc attttctgca atgcttttga
     2581 agaaatgttc gaacagacta gcatccatag tccccactga agaagaatta agctttacat
     2641 tccagacaaa gccaatgcga ttgctaaaat caatgcaaac acgagccaga gcctcgtcca
     2701 taggaacaaa ataaaaaccg taacgctcta ttcctttttt atctcctaga cactcgacca
     2761 aggcttggcc aagaacaata ccaatgtctt caatgctatg atgcatatcg acggcaacat
     2821 ctcccttaca aactaagtcg agcgtgaagc ccccatgcac ctgaaacaaa tcaagcatgt
     2881 gatctaaaaa agcagaacca gaatcaatct tgcctctaga tgctttatct aaattcaagc
     2941 ttaaatcgat ttgagtttcg tttgtctttc tcgaaatttt agaacaacgc atattagcga
     3001 actcctggaa taatttgacc attaaagact ctaaatctag tgactaaccc atttttcatc
     3061 tttggtaatg ctgtttcttt gccatttaat gtcatttttg caatcgctac tggatctcca
     3121 ataatcacat acaacgtatc agaagtttca tagactaact tgccaccctc tcgagaaata
     3181 ttagcctctt tcaaaaatgc agtgtctgat tcattgcgtt ttaaacccac ccatgagttc
     3241 gcaactcctg aaccaatcaa aacaaaagaa gtgttttttg tattgacttg aactttttct
     3301 tttgaagaag aagaaatgaa aatggtagcc gatgctggta attctttctt tttagaaatt
     3361 gtatccacag cggttgtacc taccaccgct tgaatagaat cagaagagag agaatctttt
     3421 actaaagagt cgcgtactaa agaatctagc accactaatt ctgctccgtc atgaattaaa
     3481 gtagagcttt ccacactttc ttcagagtct tgtggtgcca ctgaagattc cttcatcaaa
     3541 gggttattag aaacagactc ctctgttttt tcaaattgat ttacaaagtt catcaaaaca
     3601 aaaaaggcta gtccaaggac gacaacaatg attggcatta aaaagctttt cttttctcgt
     3661 tcactagaga ctgcggtttc tttttctacc acttgttgtt ccgcataatc aggagagccc
     3721 ttttctttca agtaactaga taaaacttct ttgtaatcaa gttcaagaat tctagaaatg
     3781 gaattcaaat aacctcgaag ataagcatct acaggaaatt tgctccaatc acccgcttca
     3841 atatttttca tgtaagttat ggaaatacgt gtttcacgta ccatatcttc aatagtatac
     3901 cctcttgcta ctctagcacg ggttaaataa gcagctaaag attcattttc gtttttactt
     3961 tcaaacactt tatcatttac gggcataaaa acttttcaac ctcttttaac aaatcaagcg
     4021 tagtggcgac cggaagaccg actacgttat aataacaacc ttgaatggat tttaccaaag
     4081 acgctccgcg cccttgaata ccataagcac cagctttatc cattggctct aaagaattta
     4141 catattctaa gagatcttgt gggtcattgt ttctaaaaaa cacctctgtt tcttctattt
     4201 ttgattttat gacttctcca ccataggcaa aagcgatccc tgagcgtact tgatggctcc
     4261 tgccatttaa ggattgaagc aaaaaataag cgtcttctgg tgtttttggt tttcctaaag
     4321 ggactccatc tctaaaaaca agagtatcac aaccgagaac tatagaattc gggtgagatt
     4381 ttgaaacgtc ttttgccttc atttcagcat ataaaagggg ccattgcatg ggagag
//
LOCUS       DAOOBB010000166         4358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_22639_length_4358_cov_4.143621, whole genome shotgun sequence.
ACCESSION   DAOOBB010000166 DAOOBB010000000
VERSION     DAOOBB010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22639_length_4358_cov_4.143621"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            284..2014
                     /gene="ilvB"
                     /locus_tag="PLT31_07535"
     CDS             284..2014
                     /gene="ilvB"
                     /locus_tag="PLT31_07535"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HPW95019.1"
                     /translation="MNELNTKKTLNGAEVTLECLKREGVEIIFGYPGGSAIPLFDALY
                     DSNMKVVLSRHEQGATHMADGYARQTGKVGVALVTSGPGATNTFTGIYTALMDSIPIV
                     VLTAQTTTPNLGKDAFQECDTSGMTFAAVKHSYLVKKSNDLPRILKEAFHVARTGRPG
                     PVLIDLPKDVLAGVCSASFSDEMDLPGYKIPTYADLSAVEQAAELLKTSKKPLLLVGH
                     GAMISNASRQVKELAEKLNAPVTSTLLGLGVFPADHDLSLGMLGMHGTVYANKAVLDC
                     DLIFSIGSRFDDRITGKLEEFCKNAKIIHLDIDPAEQGKVLEPDVFLCGDAKLVLEQL
                     LPMVPRLDSAEWVKTVQTWKKRYPLTYPKQGGLRMQHVIHTLSELTKGKAILNVDVGQ
                     HQMWAAQFFNANYPRQLHNSGGAGTMGFGFPSSIGASFGNTTGWPVCCIAGDGGFQMT
                     LSELATAAIHKLPIKIFVLDNKYLGMVRQWQDMFYDKRYSSVDLVGNPDFVKLAEAYG
                     IPGLRLKRSADAERMIQKALDYNDGPILIHCECEKEDNVFPMIPAGAPITSMITEMPK
                     TKLEKPTGST"
     gene            2016..2519
                     /gene="ilvN"
                     /locus_tag="PLT31_07540"
     CDS             2016..2519
                     /gene="ilvN"
                     /locus_tag="PLT31_07540"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HPW95020.1"
                     /translation="MNPIQEKAHSISLMVANRPGVLVRISLVFSRRGYNIDSLVVSPT
                     LDPNFSRMNIVARGNPTILEQIIKQLEKLVDVVQAKDHTNMNVVEKELALIKVKCSPE
                     QRTEMLQLCDHFKAVTVDMTETSMIIQITGNTDKIDAMKSLSQKFEIIEYIRTGKVIM
                     LRGEDKT"
     gene            complement(2633..3763)
                     /locus_tag="PLT31_07545"
     CDS             complement(2633..3763)
                     /locus_tag="PLT31_07545"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HPW95021.1"
                     /translation="MKKYYSMVLILLLASFTNAFAEWSIFLLIGQSNMAGMAATPSSI
                     DLEELEQVRVLGFKDCSNPSRVYNEWDIASPPLHNCTEGVGPGDWFAKTLVQEGYPDT
                     IGLVPVAISGAGIDLFRKGISYSYNYTPFENNRAGAGYDWMLTKLRKALEKGSLKGIL
                     FHQGETDCSGWGGNPTTWVSRVEDVISDIKADLNIDYDLPFIAGHLSDTENNFKQMNP
                     YIDQLPSVIPNTLVVSSEGLSRGRDNIHFTTESQREFGKRYANAFLNWQKENSSSSTS
                     ISSSSVTESSSSESISSSSTEMMFVKSMDVESSMWNAYVNGATIQIENISIGSTIQLM
                     DVQGNVLLKTKASSKENSFVMNRSGSFILKVGNKIQQLNIKK"
     gene            complement(3920..>4358)
                     /gene="fabZ"
                     /locus_tag="PLT31_07550"
     CDS             complement(3920..>4358)
                     /gene="fabZ"
                     /locus_tag="PLT31_07550"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF000582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HPW95022.1"
                     /translation="LYDESVVMQILPQRDPFRFISYVKEVVLDVENPQIVAVREVKAD
                     EYYFKGHFPQEPIMPGVLQIETMAQAGCILGLLAHEKEVEGKRPAFMGVQECRFRKPV
                     VPGDILTVKVQLNKFRKGIMFFEGSIYRGEELVSNATLTAAMV"
BASE COUNT         1456 a          814 c          758 g         1330 t
ORIGIN      
        1 aaaaagcatt tcttttataa aaaaaaagcc tgttttaaat ctcttcaatc aaaaaataaa
       61 cctactttca acagaataag cttgttttta gattcataaa tttaatttta acccaagttc
      121 atgttatttt tctttgattt taaagtttta aagatcttct taaataaagt ttttcaaaaa
      181 tcattacata aacagcacaa aaaatatttt tcattaattt taattttaaa aatcaaataa
      241 attattctat attttaataa gttcttttca aaacgagttc aaaatgaatg aattaaacac
      301 aaagaaaaca ttaaacggcg ccgaagtgac tctcgaatgc ttaaagagag aaggtgttga
      361 gattattttt ggctatcctg gtggatcagc tatcccatta tttgatgctc tttacgactc
      421 taatatgaag gtcgtactat caaggcatga acaaggtgcc acacatatgg cagacggtta
      481 tgctcgtcaa acaggaaaag tgggagtcgc attagtgaca agtggtcctg gtgcaaccaa
      541 tacatttaca ggcatttata ccgcgttaat ggattctatt cctatcgtcg ttcttacagc
      601 acaaactaca actccaaatt tagggaaaga cgcattccaa gaatgcgata ctagcggaat
      661 gacttttgct gccgtaaagc attcttattt ggtaaaaaaa tcaaacgatc ttcctcgtat
      721 cttaaaagaa gcctttcacg ttgctagaac aggtcgtcca ggccctgttc tcattgattt
      781 acctaaagat gtgcttgctg gcgtatgcag cgcttctttt tctgatgaaa tggatttacc
      841 tggttataaa attccgacct atgctgatct ttcggcagta gaacaagcgg ctgaattatt
      901 aaaaacatcg aaaaagcctc tattattagt aggtcatgga gcgatgattt caaatgcttc
      961 aaggcaagtg aaagagctag cagaaaaatt aaacgctcct gtcacaagca cgcttctcgg
     1021 gttaggagtg ttcccagcag atcatgattt atctcttggg atgcttggca tgcatggtac
     1081 tgtttatgca aataaagcag ttctggattg tgatttgatt tttagtattg gatctcgctt
     1141 tgacgatcga atcactggaa aactcgaaga attttgtaaa aatgcaaaaa ttattcactt
     1201 agatattgat cctgctgaac aaggaaaggt gttagaacca gacgtgttct tatgtggaga
     1261 tgcaaaactt gttttagaac aattgcttcc aatggtacct cgtcttgatt ctgctgaatg
     1321 ggttaagacc gttcaaacat ggaaaaagag ataccctcta acctacccta aacaaggtgg
     1381 acttcgcatg caacatgtga ttcatacctt atcagagtta accaaaggaa aagccatatt
     1441 aaatgtggat gtaggccaac atcaaatgtg ggcagctcaa ttcttcaatg ccaactaccc
     1501 tcgccaactt cataattcag gtggagcagg tacaatgggt tttggcttcc ctagttctat
     1561 tggcgcttcc tttggaaaca ccacaggctg gccagtttgt tgtatcgctg gagatggcgg
     1621 ctttcaaatg actctatctg aattagcgac agcagctatt cataaattgc ctattaaaat
     1681 ctttgtcctt gataacaaat atctaggtat ggtacgtcaa tggcaagata tgttctatga
     1741 caaacgttat tcaagcgtag atcttgttgg aaatcctgat tttgtaaaat tagcagaagc
     1801 ctatggaatt ccaggacttc gcttaaaaag aagtgctgat gcagaacgta tgattcaaaa
     1861 agctctcgac tataatgatg gccctatttt gatccattgt gagtgtgaaa aagaagataa
     1921 cgtgttccct atgattccag caggagctcc aattacttca atgatcacag agatgcctaa
     1981 aacaaaactt gaaaagccta cagggagtac ctgatatgaa tcctatacaa gaaaaagctc
     2041 attccataag tcttatggtg gcaaatagac ctggagtact tgtacgtata tcccttgttt
     2101 tttcaagacg cggatataat atcgattctc tagtcgtttc tccaacttta gatcctaatt
     2161 tcagccgtat gaatattgtg gctcgaggca atcctactat tttagagcaa atcattaaac
     2221 agctcgaaaa gttagtggat gtggttcaag ctaaagatca tactaatatg aatgtagttg
     2281 agaaagaact tgccttaatt aaagtgaagt gttctccaga acaacgcacc gaaatgctcc
     2341 aactatgcga ccattttaag gctgttactg ttgatatgac agaaacgtct atgattattc
     2401 aaattactgg taatacagat aagattgacg cgatgaaaag cttaagtcaa aaatttgaaa
     2461 tcatagaata cattagaaca ggaaaagtga ttatgcttcg tggagaagat aaaacttaat
     2521 tctgcttttt ataagttcaa aaaaatgctt tccgaaaaat aaaccggaaa gcattttttg
     2581 ttttgtgaga gtaagaagga aactatgaat tttaaatttt gtttataacg aattattttt
     2641 tgatatttag ttgttgaatt ttattaccca cttttaaaat aaatgaacct gaacgattca
     2701 ttacaaaaga attctctttt gaagaggctt ttgtttttaa taaaacattt ccttgaacgt
     2761 ccatgagttg tattgtagaa ccaattgaaa tattttcgat ttgtatagta gccccattta
     2821 cataagcgtt ccacatagag gattcaacat ccatcgattt gacaaacatc atttcagttg
     2881 aagaactact tatactctct gaagatgagg attcggtgac cgaagaagaa gagattgaag
     2941 ttgatgaaga actattttcc ttctgccaat tcaagaaagc gtttgcataa cgttttccaa
     3001 attctctttg cgattctgtg gtgaaatgaa tattatctct tcctctagac aagccttctg
     3061 aagaaaccac tagagtattt ggaatcactg atggaagttg atctatgtaa ggattcattt
     3121 gcttaaaatt gttctctgta tcagataaat gcccagcaat aaaaggcaaa tcatagtcga
     3181 tatttaaatc tgctttaata tcagaaatca catcttctac tcgagaaacc caagtcgttg
     3241 gattaccacc ccagcccgaa caatctgtct ctccttgatg aaaaagaatt cctttcaaac
     3301 ttcctttttc aagagctttt cttaatttcg taagcatcca atcataacct gctcctgctc
     3361 tattattctc aaaaggggta taattgtaag aataagaaat ccctttacga aataaatcaa
     3421 taccagctcc agaaatggcg acaggaacta atccaatcgt atcaggatac ccttcttgaa
     3481 cgagcgtttt agcaaaccaa tctccagggc ccacgccttc agtacaatta tgaagaggtg
     3541 gtgacgcaat atcccattca ttataaactc gagaaggatt agaacaatcc ttaaagccta
     3601 atacacgaac ttgttctaat tcttcaagat caatagatga aggggtcgca gccattccag
     3661 ccatattaga ctggcctata agtaaaaaaa tactccattc ggcaaatgca tttgtaaaac
     3721 tagcgagtaa taaaatcaac accatagaat aatatttttt catattaaac cactcctatc
     3781 ctataattaa tcagaaaatt attaagaaaa gctggatctc taacgtaaaa aaaaattaca
     3841 cgttgtatat taaaacaaca aaaaaggtca atactctaag tattgacctc tttggaaaaa
     3901 caaacttaca cgatgtgtat taaaccatcg cagcggtgag cgtagcatta gaaacgagct
     3961 cttctccgcg ataaattgag ccttcaaaga acataattcc ctttctgaac ttattcagtt
     4021 gcacttttac cgttaaaata tccccaggaa ctacaggttt tctaaaacga cattcttgaa
     4081 cacccataaa agcaggtctt ttcccttcga cttctttttc gtgagctaat aaaccgagaa
     4141 tacagcccgc ttgagccatc gtctcgattt gcaaaacacc aggcataatt ggttcttgag
     4201 gaaagtgtcc cttaaaataa tactcatcgg cctttacttc tcgaacagca acgatttgtg
     4261 gattttctac atctaacacc acttctttta cataagaaat aaaacgaaaa ggatctctct
     4321 gaggtaaaat ttgcatgacg acagattcat catataaa
//
LOCUS       DAOOBB010000167         4357 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_22646_length_4357_cov_4.648768, whole genome shotgun sequence.
ACCESSION   DAOOBB010000167 DAOOBB010000000
VERSION     DAOOBB010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 4357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4357
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22646_length_4357_cov_4.648768"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..1247
                     /locus_tag="PLT31_07555"
     CDS             57..1247
                     /locus_tag="PLT31_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HPW95023.1"
                     /translation="MSKVLIIGAGGVANVVARKCAQNSSVYSELLIASRTQSKCDAIA
                     KDIHNIPVNTAKVDADKSEEVAALIRDFKPELVMNIALPYQDLTIMDACLKEGVHYLD
                     TANYEPKDVAHFEYSWQWAYHERFKKAGIMALLGSGFDPGMTNVYTAYAKKHHFDQID
                     TLDIVDCNAGNHGKAFATNFNPEINIREITQNGRYYENGKFVEIPPLSMHQTINYPEV
                     GDRESYLLFHEELESLVKHFPEIKRARFWMTFGQAYLTHLQVLQDVGMTRIDPVRFNG
                     VDIIPLEFLKALLPEPGTLGENYSGKTCIGCQVTGIKEGKPKTYFVYNVCDHAESYKE
                     VKAQAISYTTGVPAMLGGSMILKGLWKGEGVFNMEQFDPDPFMAEIGQWGLPWKELLN
                     VKLV"
     gene            complement(1350..1781)
                     /locus_tag="PLT31_07560"
     CDS             complement(1350..1781)
                     /locus_tag="PLT31_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3332 family protein"
                     /protein_id="HPW95024.1"
                     /translation="MKKRLIALSCAAILFSGCYGNYALFGKVQKFTGSFGDKWINSVV
                     NLVAWIVPVYEICLLGDLLIFNTIEFWTGSNPVASGDTYFEKDAQGNAVTAVKNADGS
                     LSVSLTDVQGHQADLVLQRDADVVRALDQQGNVVAQYNIEK"
     gene            2264..3793
                     /locus_tag="PLT31_07565"
     CDS             2264..3793
                     /locus_tag="PLT31_07565"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPW95025.1"
                     /translation="MKQILFFAFFVFFTISTPFAAEPLTVWIMPNGASPQEKMEQELQ
                     QFTKTTGIKTKVVVLDWGEAWSRISNALSTGKGMPAVLQLGTTWIPYFASRGELFELN
                     PFLSQIDTSRFEEVSFNTTHIDGDTSIYSIPWFIDVRAILANERILKELGIKAEDIKT
                     YEGFLKTLKKIKAASLKLDDGTPIQPYAFPGRNDWNIPHNFGPWVWSNGGSFLVKDSN
                     SYRSNLLAPNTLQGIARYLNFILDSIVDPSNLNLNTAQISQQFNNGELAFILNTSEIV
                     MQTRYQAQDGGLQNSRIGNDGIIVFPVPSGSAGSISFIGGSNLAIPAKYKNKKQALQL
                     LLFLTQDSFLDAYTKQIGFLPPSKKVLESWSKDSVYKVLVECLKNGQNYRSIPQWGEI
                     ENLLGRTFNNIWVSMEMKDLYSEEKIYSILKENDSIINTVLGAEQSKQRMSLDEFKTI
                     WASILNEVNTEENLSSEEKTEEKVAKSEEKSYFDKKISIGIFLLMIFFGFIFAYQRKK
                     K"
     gene            3868..>4357
                     /locus_tag="PLT31_07570"
     CDS             3868..>4357
                     /locus_tag="PLT31_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95026.1"
                     /translation="MLIIAITLIVGVSGAFFVIYQRESLEQSVFEKNSSDLKQVMNSI
                     TQELDQFGSQLSLLSKTTSVQQMDSIVSASFLKSFDVSTLFKIGEYVTLYDRHHQFIC
                     DNSMIGTSSPHQPFTAFNSVTPHRPYTSAWYSEEDFIPNKFFAIAVGDRAHASGSLVA
                     NFS"
BASE COUNT         1487 a          793 c          731 g         1346 t
ORIGIN      
        1 acgagacatt gtttattaaa ataattaaat tttgtcaaaa ctatatcgga gatctcatgt
       61 ctaaagtact tatcattggc gctggtggcg tagcaaacgt agtcgcacgc aagtgtgcac
      121 aaaatagttc cgtttattcc gaactactga tcgctagtcg aacacaatcc aaatgtgatg
      181 cgattgccaa agacattcat aatattcctg taaatacagc aaaagtagat gccgataaat
      241 cagaagaagt cgctgcacta attagagatt tcaaaccaga actcgtaatg aatatcgctc
      301 ttccctatca ggatctcacc atcatggatg cctgtttaaa agaaggcgtt cattatcttg
      361 atactgcaaa ctacgaacct aaagacgtag ctcactttga atatagttgg cagtgggcct
      421 accacgagcg ctttaagaaa gcaggcatta tggctctcct cggctcaggc tttgaccctg
      481 gaatgacaaa cgtctacacc gcctatgcca aaaagcatca ttttgatcaa atcgatactc
      541 ttgatattgt agactgtaat gcagggaacc atggaaaggc ttttgccact aatttcaacc
      601 cagaaattaa catccgagaa atcacacaaa atggtcgtta ctacgaaaat ggcaagtttg
      661 tggaaattcc tccattgtct atgcatcaaa caatcaatta tcctgaagtt ggtgatcgcg
      721 aaagctattt acttttccac gaagaactag aatctctcgt caaacatttc ccagaaatca
      781 aaagagcccg tttctggatg acttttggcc aagcttacct cactcactta caagttttgc
      841 aagatgtagg catgacccgc atcgatcctg ttcgctttaa tggcgtagat attattcctc
      901 ttgaattctt aaaagcactt ctaccagaac ctggtacatt aggtgaaaat tacagtggaa
      961 aaacatgtat cggttgccag gttacaggta taaaagaggg taagcctaaa acttactttg
     1021 tttataatgt ttgtgaccat gccgaaagct acaaagaagt gaaagctcaa gccattagct
     1081 acactacagg agttcctgca atgctaggcg gatccatgat tcttaaagga ttatggaaag
     1141 gtgaaggcgt gtttaatatg gaacaatttg acccagatcc atttatggct gaaattggtc
     1201 aatggggtct tccttggaaa gaattattaa acgtaaagct tgtttaatta atttgtaccg
     1261 cgagctaaat ctcgcggttt ttttatgtaa tttttgataa aaaaatggcc tcgatgatat
     1321 cgagaccgtt ttttaattcc aaagaaaaat tatttttcaa tgttatattg agcaacaaca
     1381 ttgccttgtt gatcgagagc acgaacaaca tcagcatcac gttgaagaac gagatcagct
     1441 tgatgacctt gaacatctgt aagagaaaca gataaagaac cgtctgcatt cttaacagct
     1501 gttacggcat taccttgagc atctttttca aaataggtgt caccagaagc tactggattt
     1561 gaaccagtcc agaattcaat ggtattgaaa atcaataaat caccgagtaa acaaatttca
     1621 taaacaggaa caatccatgc cactagatta acaacagagt taatccactt gtcaccaaaa
     1681 gatcctgtga acttctgaac cttaccaaat aatgcatagt ttccatagca accactgaaa
     1741 agtatagcag cgcatgaaag ggctattaga cgttttttca tattgaactc cttaatagaa
     1801 tgaaaaattt gaatgaatat ataaaaataa gtcaactctt tcatataaaa aaaagaaata
     1861 cttattatta ctctatacaa cgacatccat attcaaatga cccatgttga atttcaagag
     1921 gtgattatta taaatggcat aaaaacgggt aatttacaaa ttatttcaat gtttttaaat
     1981 aaaaaaaact ttatcctata tgtgatatat atcacatata cctagaataa taaaactcta
     2041 taaacttctt ttatgaaaaa cacaaaacac tatcgtacaa attcgatata aaaagcactt
     2101 ttttataatc ccgtaaaaaa agatttttta caaattatag ttctaaatga atttgtacag
     2161 taaaaaatct gttttatttt tttaaatgtg ttattcaaag aaaattactt ataaattata
     2221 ttaaatattg tatattcgat atattatgat tggaggttgt ttgatgaaac aaattctttt
     2281 tttcgctttt ttcgtttttt ttaccatttc tactcctttt gcagcagaac cattaactgt
     2341 ttggattatg ccaaatggtg cttctcctca agaaaaaatg gaacaagaat tacaacagtt
     2401 tactaaaaca acagggatca aaacaaaagt cgttgttcta gactggggtg aagcttggag
     2461 tcgtatatct aacgccctta gtacaggcaa aggaatgcct gccgttcttc agcttggaac
     2521 cacctggatt ccttattttg cttcccgtgg tgagctcttt gaattaaacc catttctttc
     2581 tcaaatagat acttctcgat ttgaagaagt cagttttaat acgactcaca ttgatggaga
     2641 tacttccatt tattctatac cttggtttat tgatgttcgt gctattcttg ccaatgaaag
     2701 gatcttaaaa gaattaggca ttaaagctga agatataaaa acctacgaag gttttttaaa
     2761 aacattaaaa aagatcaaag cagcctcgct aaaactagat gatggaacac ctattcaacc
     2821 ctatgcattt cctggtagaa atgactggaa cattccacat aactttggtc catgggtttg
     2881 gtctaatgga ggctctttct tagttaagga ttctaattca taccgttcta atttactcgc
     2941 tccaaatacg ctgcaaggca ttgctcgcta ccttaatttt attttagatt ctattgtaga
     3001 cccctccaat ttaaatttga atacagctca aatttctcag caatttaata atggagagct
     3061 agcctttatc ttaaacacct ctgaaattgt aatgcaaact cgttatcaag ctcaagatgg
     3121 cgggttacag aactcaagaa taggtaatga tggcattatt gtattccctg taccaagtgg
     3181 ttctgctgga agtatcagtt ttattggagg aagcaacctt gccattccag caaaatacaa
     3241 gaacaaaaaa caagctcttc aacttttact gtttttaact caggattcat tcttagacgc
     3301 ttatacaaaa caaattggtt ttttacctcc ctcaaaaaaa gtacttgaat cttggtcgaa
     3361 agattccgtt tataaagtac ttgtagaatg cttgaaaaat gggcaaaatt acagaagtat
     3421 tcctcaatgg ggagaaatag aaaatctatt aggaagaacc tttaacaata tttgggtttc
     3481 catggaaatg aaggatctct attctgaaga aaaaatttac tctattctaa aagagaatga
     3541 ttctataatc aataccgttt taggtgcaga acaatcaaaa caaagaatgt ctctagatga
     3601 attcaaaaca atctgggcaa gcatcttaaa tgaagtcaat acagaggaaa atctttcttc
     3661 agaggaaaaa acagaggaaa aagtcgccaa aagcgaagaa aaatcttatt ttgacaaaaa
     3721 aatatccatc ggaatctttt tattaatgat attttttggt tttatctttg catatcagcg
     3781 aaaaaagaaa taaactatta ctaaaatatg gtagaaaaaa agtactctat acgcttaaga
     3841 aagagcatca tatttagaag ccttcttatg ctaattatag caatcactct tattgtagga
     3901 gtgagtggtg ctttttttgt catttatcaa agagaatcgc tagagcaaag tgtatttgaa
     3961 aaaaattcaa gtgatctaaa acaggtcatg aattccatta cacaagaact agatcaattt
     4021 ggaagtcaac tctcgttact atctaaaact acttctgtgc agcaaatgga ttccatagtt
     4081 tctgcaagtt tcttaaagag ttttgacgta tctactctgt ttaaaatagg agaatatgtc
     4141 actttatatg atcgccatca tcaatttatt tgtgataatt caatgattgg cacctcttcg
     4201 cctcaccaac ccttcactgc tttcaattca gtgactccgc ataggccata cacctcagct
     4261 tggtactcag aagaggactt tatacctaat aaattctttg ccattgccgt tggtgacaga
     4321 gctcacgcta gtggatcgct agttgccaat ttttcat
//
LOCUS       DAOOBB010000168         4354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_22665_length_4354_cov_3.774366, whole genome shotgun sequence.
ACCESSION   DAOOBB010000168 DAOOBB010000000
VERSION     DAOOBB010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22665_length_4354_cov_3.774366"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3171
                     /locus_tag="PLT31_07575"
     CDS             <1..3171
                     /locus_tag="PLT31_07575"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPW95027.1"
                     /translation="MGYTALLMTLVLILIAVFYTLGLGWISYCLILLFSIFLSIESPA
                     KLGIQKELFGVKRLTLSNAYQSSFSILGVVAVSFVTIGFSPEETSFLKYEILPWLFVI
                     IGAIATLATFKIPEVTPPSKYMKLRSSRRTISSTWSNPLLRLCIIGLAFFWGTAQIFI
                     LLSQDISGMHIVSVLRNTLIFSAIGLVFGFLAAARASRNFIETGLIPTSALGAAVMMF
                     LSAFISNELIQALVYGVIGFCAGIFFILVKALLQQNTRPDTAGRIIAVANMMQMLFLF
                     TFLTLQVVAMSFISVSIDVFFIPLAVIAFIGFSLSLAKTPQTLLRAFLRLFFFFRYRL
                     KPIGIENIPETGPVLLVGSHYSFIDWAVLQMASPRPVVIASNRSRYERWYLRLIRRRL
                     GMIFIDRRNPEPSMEKIKNALSEGKAVVIFPEGEVSKMPFISRFSIDYTEALHETGVP
                     IVPFYIYGVWGSPYSHVSDSIMFPNKVARFISVGFAKPLPPNTKEDKIRDTLRDLSID
                     VWEQSISHYKRIAPLVVRAMKARVRFKVAIYNFTGEHLSGYEFVRKILVLTRLINKVT
                     RSEKNVGLMFPPSPEGFASFIALIAKAKTSVNLNYSASPDIIMGCIQKTEVKTVLTSR
                     HFFEKLCQKNSLFNNIKKDCKLIFVDDFFASKSLLTWGYYHLIATLLPSFLIEKIFFN
                     KTLLSDTAAILFSSGSEGVPKGIMLTHKNFICNVLQTDAVVRLRPTDVVLSELPLFHS
                     FGLTANILLSLVNGVPSIPCPDPTDIKTMARVCAEFKATILMGTPTFLRAFTVNRWVH
                     PMCLDYMRLVLAGAEKMRPELRESFRLKFGKEVYEAYGCTETTPVATMNSADILLDDF
                     LTMEKCNEMGSVGMPVPGTRLRIVDPVTNEDLPVGEEGMALVGGPQVMKGYYKEPEKT
                     DEAVIVLDGKRWYRTGDKAFVDQDGFLTIVDRYSRFAKLGGEMVSLGAVDLRVGDTKI
                     LDGCEFVSTSIPDDVKGECIVLLFVGDKDTHTVSMALRKSGMPPLMIPGKVYAVEAIP
                     KLGSGKWDFTSIKKMAVELSKKSSLR"
     gene            3477..4082
                     /locus_tag="PLT31_07580"
     CDS             3477..4082
                     /locus_tag="PLT31_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014544947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HPW95028.1"
                     /translation="MAEDLQYLIDRIQKDAIDKADNDATSILTAAKEKAAQIIKEAEA
                     EAKAKLEQADKDAQVYTERSERALEQSARDILITVGKSLEKMILDILDLQVEKALSES
                     TIKELLLSLAKNYSSDVEVALSESDVNKLTSFILGEFKKQLSAGVQVESDSSVRFGFR
                     VKLDGGKVSHEFTNEAMADALSALLRPQLAKVVTKAASQGK"
     gene            4082..>4354
                     /locus_tag="PLT31_07585"
     CDS             4082..>4354
                     /locus_tag="PLT31_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95029.1"
                     /translation="MSNAVYLVSSLPTLQFGDTPPFSLDEFRRQCEGVISAYEMEALE
                     ALLAGKSHSDSFVSAYFARETLLKNIAGKMRAAAWDSDIRFSERPYD"
BASE COUNT         1263 a          748 c          848 g         1495 t
ORIGIN      
        1 atgggttata ctgcgttatt gatgacgctt gttttaattt taatcgctgt cttttatact
       61 ttaggtttag gttggatctc ttactgtctt attttattgt tttcgatatt tctttctata
      121 gagagccctg caaaattagg tattcaaaaa gaattatttg gggtaaagcg attaacttta
      181 tctaatgctt atcaatcttc tttttccatt ttaggggtag tggcagtctc gtttgtgacc
      241 attgggtttt ctcctgaaga gacgtctttt ttaaaatatg aaatattacc ttggcttttt
      301 gtaatcatag gagcgattgc gacattagct acttttaaaa ttccagaagt aacgcctcct
      361 tcgaagtaca tgaagcttcg gtcttccaga agaacaattt cttctacgtg gtcgaacccg
      421 cttctcaggc tttgcatcat tggccttgct tttttttggg gaacggctca aatatttatt
      481 ctattatccc aagacatttc tggaatgcat atcgttagcg ttttacgcaa cacgctcatt
      541 ttttccgcga ttggtttagt ttttggcttt ttagctgcag caagagcctc tagaaatttc
      601 atagaaacag ggcttattcc tacgtcggca ttaggtgctg ccgtcatgat gttcttatct
      661 gcctttatct caaatgaatt gatacaagca cttgtctatg gggtaattgg tttttgtgct
      721 ggtatctttt ttattctcgt gaaagctcta ttacaacaaa atacaagacc tgatactgct
      781 ggtcgtatta ttgctgtcgc caacatgatg cagatgcttt tcttatttac gtttctcact
      841 cttcaagtcg tcgcgatgtc ttttatctcg gtttccattg acgtcttttt tatccctctt
      901 gcagtcattg cttttattgg ctttagttta tcattagcta aaacacctca aacccttttg
      961 agagcatttc ttcgtttatt tttctttttt cgctatcgcc taaaacctat tggtattgaa
     1021 aatattccag aaacaggtcc tgttttatta gtgggatcac actatagttt tattgattgg
     1081 gctgtgttac aaatggcttc tccaaggcct gttgttattg caagtaatcg aagccgctat
     1141 gaacgttggt atttacgttt gattagaaga cgattaggca tgatttttat tgatcgtcga
     1201 aatcctgaac cttcaatgga aaaaatcaaa aatgcgttat cagaaggaaa agctgttgtc
     1261 attttccctg aaggtgaagt ttctaaaatg ccttttatca gtcgtttttc aattgattat
     1321 acagaggcac ttcacgaaac aggtgttcct atagttcctt tttatattta tggagtatgg
     1381 ggctctcctt atagccatgt ttctgatagc atcatgttcc caaataaagt ggctcgattt
     1441 atctcggtag ggtttgcaaa acctttacct cctaatacta aagaagataa gattagagat
     1501 actttaagag atctatcaat agatgtttgg gagcaaagta ttagtcatta taagcgaatt
     1561 gctccacttg ttgttcgagc gatgaaagca cgagttcgat ttaaggtggc tatttacaat
     1621 ttcacaggcg aacatttgtc tggttatgag tttgtacgaa aaatattagt cctaacacgt
     1681 ctaattaaca aagtcactcg ttcagaaaaa aatgtggggc tcatgttccc tccttctcca
     1741 gaggggtttg cttcctttat tgcattaatc gctaaagcaa aaacaagtgt taacttaaat
     1801 tatagtgctt ctcctgatat tatcatgggt tgtattcaaa agacagaagt caaaacagta
     1861 ttaacctcgc gccatttctt tgaaaaatta tgccagaaaa attctctttt caataatata
     1921 aagaaagatt gcaagttgat ttttgtggat gatttttttg cttcaaaatc tttattgaca
     1981 tggggctatt atcatttaat agcgacgctc ttaccatcat ttttaattga aaaaatcttt
     2041 ttcaataaaa cgttattatc ggatactgct gctattttat ttagctctgg gtcggaaggg
     2101 gttcctaagg gaatcatgtt aactcacaaa aatttcatct gtaatgtttt acaaacagat
     2161 gccgtggtta gattacgccc cacagacgtg gttctttcag aattaccatt attccattcc
     2221 tttggattaa ccgcgaatat tctgctcagt ttggtgaatg gagttccttc aattccatgc
     2281 ccagatccaa ctgacattaa gacgatggct cgtgtttgcg ctgaatttaa ggccactatt
     2341 ttaatgggta cacctacatt cttacgagcc tttacggtga accgttgggt tcatcctatg
     2401 tgtttggatt acatgagact tgtgcttgct ggagctgaaa aaatgcgtcc agagttaaga
     2461 gaatctttta gattaaaatt tggtaaagaa gtctacgagg cctacggctg tactgaaacc
     2521 acaccagtgg ctacgatgaa ttcagctgac attttattag atgacttttt aactatggaa
     2581 aaatgcaatg aaatggggag tgtggggatg cctgttcctg gaactcgtct tcgcattgtt
     2641 gatcctgtga ctaatgaaga ccttccagtc ggtgaagaag gaatggcttt ggttggtggc
     2701 ccgcaagtga tgaaagggta ttataaagag ccagaaaaaa cagatgaagc agtaattgtg
     2761 cttgatggaa aacgttggta tcgtactgga gataaagcct tcgttgatca agatggtttc
     2821 ttgactattg tggatcgcta tagccgcttt gccaaattag gtggtgaaat ggtgagcctt
     2881 ggagctgtgg atcttcgagt aggtgataca aaaattcttg atggatgtga attcgtatcg
     2941 acttctattc cagatgacgt caaaggagag tgcatcgtgt tactttttgt tggcgataaa
     3001 gatactcata cggtctccat ggctctacgt aaatctggta tgccgccgtt aatgattcct
     3061 ggaaaagttt atgctgtgga agcaattcca aaactcggca gcggtaaatg ggatttcacg
     3121 agcattaaaa aaatggccgt ggaattatca aaaaagtctt cattgcgttg atggattata
     3181 acagaattat tcataattct gttataaaaa aaataataat cacatcatat tgtgattatt
     3241 agaatctgat ttgaaaaaaa atgatttaaa tatgatatta tataatcatt tatcaagaaa
     3301 cgatacaata tttttttaaa aagaaaaatt aatttttttt ttatccccaa aaaagaactt
     3361 tttatcttaa ataaaaacat taaatttttt ttttacactt ttttatgttc ttttttgact
     3421 tttccttata tattcaaaat aacaatttta agcattaatc tttcggagaa catataatgg
     3481 ctgaagattt gcagtatctc attgatcgaa ttcaaaaaga tgcgatagac aaggctgata
     3541 atgatgccac ttctattcta actgccgcaa aagaaaaagc ggctcaaata attaaagaag
     3601 cagaagcaga agcaaaagca aaactcgaac aagcagataa agacgctcaa gtttatacag
     3661 aacgtagcga acgtgctcta gaacaatctg caagagatat tttaattaca gttggtaaaa
     3721 gtcttgaaaa gatgattttg gatatacttg accttcaagt agaaaaagct ctttcagaaa
     3781 gcacaattaa agaactttta ctttctttag ctaaaaatta tagttcagac gtagaagttg
     3841 ctttatctga atcagatgta aataaattaa catcattcat tttaggcgaa tttaagaagc
     3901 aattatctgc tggcgttcaa gtagaaagcg attcttcagt tcgttttggc ttccgtgtta
     3961 aactagatgg cggtaaagtc agtcatgaat ttactaacga agccatggca gatgctttat
     4021 ctgcgttgct tcgtcctcaa ttagctaagg ttgtaacaaa agcggcgtcc caaggcaagt
     4081 aatgagtaac gctgtttatc tcgtctcctc tcttcccacg ctccaatttg gagatacgcc
     4141 tcctttttct cttgatgaat ttcgtcgtca atgtgaaggg gtgattagtg cgtatgaaat
     4201 ggaagcatta gaagccttat tagcagggaa atctcacagt gattcctttg tgtctgctta
     4261 ttttgctcgt gaaacgcttc taaaaaatat tgcaggtaaa atgagagctg cggcttggga
     4321 ttcagatatt cgtttttcgg aacgtcctta tgat
//
LOCUS       DAOOBB010000169         4279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_23178_length_4279_cov_4.463068, whole genome shotgun sequence.
ACCESSION   DAOOBB010000169 DAOOBB010000000
VERSION     DAOOBB010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23178_length_4279_cov_4.463068"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1478)
                     /locus_tag="PLT31_07590"
     CDS             complement(<1..1478)
                     /locus_tag="PLT31_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95030.1"
                     /translation="MHFTPYISTFFIFAALVFSSCAYQPIRVPEYGLSEKSMEEVQAD
                     SIYKKDLAYVQKNDTDQDAIDKYISQAFYELEYIKYSIVDLNSAWLLDSLNPHVYMGF
                     ALHEMANGNTEKAIRYYKKYRELYEKTPIPRPKKIEEAPKNTHPIYVYGKLISLKKDN
                     GHDGHWEINWPAIRVSGDDEKIDRYLKNSNVLPENERYVLKQNLYMNRKRPFYTVGEI
                     RLQSVFDEESGIWKQVSKDSIFRFEYVERQGLYLNEEQKSLVTLGNTDVPDSSARVFL
                     LKPYALLSDSLAIDSYTEKIIGSENLDSPTKLRFKPYQEKTFHNSMDKPQVRSGINRS
                     LQEEKYDSMRVYLDIAEQYNKQYGSMPLNAQEYTLLFFLEEQISKDGSISKKTLKKMM
                     EKWFITGSSDSLALLLAHRLNPHHLSRTMDKKFFARSQVSPLVNINMGNLKRYLGSYS
                     SGFTIGMDCCYAYACAGMGIGPLIDESPGEVVYKNDIESIDE"
     gene            1749..1946
                     /locus_tag="PLT31_07595"
     CDS             1749..1946
                     /locus_tag="PLT31_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95031.1"
                     /translation="MDNKLKKTADNHSNADAKTTTPDDVETYFQLKKMKKWLGVQGTT
                     NVYDDEGGPLSYGDGHSGWNR"
     gene            2134..2508
                     /locus_tag="PLT31_07600"
     CDS             2134..2508
                     /locus_tag="PLT31_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95032.1"
                     /translation="MNVSQKKEILASLNWDSSVTIDELLNLVEGKSLIEAGISRESFF
                     LRCLERVPWHNLIVLWDGVENSKKLYTNNVRRGLRNGNLRRKFDFVFGILRGESVQAP
                     EWSSEYCQKLKRTFLPDRWNRA"
     gene            2585..3088
                     /locus_tag="PLT31_07605"
     CDS             2585..3088
                     /locus_tag="PLT31_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95033.1"
                     /translation="MSSVLEELKIKGFYWSNEIGFVKSMDFYSLIVQHSDFCTKLKLD
                     FVNDIVAHFGDIQNTDVYYRTDSIRNILSNKITAIFRMSAKDVVDIREICIHEKFDWN
                     EIFTEVREKELGIEPLEVVEILKGIPKTAFNSIKWIQTTDYETFMCDIEIIARDMLGL
                     TFNSLVK"
     gene            complement(3391..3516)
                     /locus_tag="PLT31_07610"
     CDS             complement(3391..3516)
                     /locus_tag="PLT31_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95034.1"
                     /translation="MMPKNTTDAKAFNEMLKSLKGSPKPDEKELRPPQFLKTTPK"
     gene            complement(3506..>4279)
                     /locus_tag="PLT31_07615"
     CDS             complement(3506..>4279)
                     /locus_tag="PLT31_07615"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPW95035.1"
                     /translation="RTSLVILSPESRIIRFNDKTLPIHNLSHTLSLIDTGISRIDFEE
                     ITPQASGFTSEDLEKIQQMLSSGDKMVWAVNVADDFCKFEFRNSQLTVFKMVSYHKNE
                     NDEEVRFEIKENSDGSRRCLHLFPAFHSLTAQGSDVCIVVDELDRSLHSNLVKQLLVS
                     FLETRNQDTRSQLILTNHDILQMDQEVLRRDEIWISERNERNGATSLTALYEYKMPKG
                     YIRKDHCLTKLYLSGQLGGIPKLNSFGGLFSEKENANDA"
BASE COUNT         1284 a          744 c          856 g         1395 t
ORIGIN      
        1 ctttcatcta tggattcgat atcgttttta tagacgactt ctcctggaga ttcatcaata
       61 agagggccta tacccattcc tgcgcacgca taggcatagc aacagtccat tcctatggtg
      121 aatccactgg agtaggaacc tagatacctt ttgaggttac ccatgtttat gtttaccaga
      181 gggcttactt gagaacgtgc gaaaaacttt ttgtccatgg ttctggatag atggtgagga
      241 ttgagacgat gggctaagag gagcgccaat gaatcgctag atccagtgat gaaccacttt
      301 tccatcattt tttttaaggt ctttttagaa attgatccgt ctttggaaat ctgttcttcc
      361 aaaaagaata gcagggtgta ttcctgtgcg ttaagcggca tgctgccgta ctgtttattg
      421 tactgctctg cgatatccaa ataaacccgc atggaatcgt acttttcttc ttgaagggat
      481 cggtttatgc cagatctgac ctggggcttg tccatggaat tgtggaatgt cttttcttga
      541 tagggcttaa atctaagctt tgtgggacta tcgagattct cgctcccgat gatcttttct
      601 gtataactgt cgatggctag agaatcgctt agcagggcgt atggtttcag caagaacact
      661 cgcgcagacg aatctggaac atcggtgttt ccgagggtta caagactttt ttgttcttcg
      721 tttaggtata gcccctgacg ttctacgtat tcaaacctga aaattgaatc cttggaaacc
      781 tgtttccaaa taccgctttc ttcatcgaaa acagattgta acctgatttc ccctacagta
      841 tagaaaggcc ttttgcggtt catgtacagg ttctgcttca agacataccg ctcattttct
      901 ggaagaacat tggaattctt aagataacgg tcgatttttt catcgtcgcc agaaacacgg
      961 attgcgggcc agttgatttc ccagtgtccg tcatgaccgt tatccttctt gagcgaaatt
     1021 agtttgccgt atacatagat agggtgagta ttttttgggg cttcttcgat ttttttaggg
     1081 cgaggaatag gtgttttctc gtagagttcc ctgtattttt tatagtaacg gattgctttt
     1141 tctgtgttgc catttgccat ttcgtgaagc gcgaatccca tgtaaacgtg ggggtttaag
     1201 gaatctagga gccaggcgct gttcaggtcc acaatggaat acttaatgta ttctagctca
     1261 taaaaggcct ggctgatgta tttgtcaatg gcatcttggt ctgtatcatt cttctgaacg
     1321 taagcgagat cttttttgta aatagaatcc gcctgaactt cttccatgga tttttcggat
     1381 agtccgtatt cgggaaccct gatgggctga taggcacaac tcgaaaagac tagagcagcg
     1441 aaaataaaaa aggttgagat atagggtgtg aaatgcattt tcctaaagat aaaaattgaa
     1501 atacaaaaaa agaatgttat caaaaaaacg cacgttattt aataacaaga acgtgtgttt
     1561 attttatttg taaagtggat tggacatggt atacttatga tgtataaatc taggtcaaat
     1621 gttattggtt tagatttttt tcgcaaaagt ttacactagt atctaaaaat cttgggtaaa
     1681 aaatggctat tttgtagcca acagagtaaa gccgaaatgg aatgtgtagt tttaaggatc
     1741 atcgccgtat ggacaacaaa ttgaaaaaaa cagcagacaa tcattcgaat gccgatgcca
     1801 aaaccacgac acctgacgat gtcgagactt attttcaact taagaaaatg aagaaatggc
     1861 ttggcgtaca gggcacaacg aacgtttacg atgacgaagg tggtcctctt tcttatggag
     1921 acggccattc tggctggaat cggtaagaaa agattaatct tgttgaatac tgatttcgtt
     1981 caaacgaaaa tagcacaagg tatacgcttt gccgagtgta atcgagcttg cttacttttc
     2041 agctaggtca aagcaatcca caaagaaacg attttatcaa gtattattat caaagttggc
     2101 agattaaaat gggtttattt atatttagca ttaatgaatg tttcacagaa aaaagaaatc
     2161 cttgcaagcc ttaactggga ttcatcggtt accatcgatg aactcttaaa tcttgttgaa
     2221 ggcaaatctc tgattgaggc tggaattagt cgagaatcct tttttcttcg ctgtctagaa
     2281 cgagttccat ggcacaattt gattgttcta tgggatggag tagaaaacag caaaaaattg
     2341 tatacgaaca atgtaagaag aggattgcga aatggaaatc tccgaagaaa atttgacttt
     2401 gtattcggta ttttacgagg agagtctgta caagctccag aatggagttc tgaatattgt
     2461 caaaaactta aacgtacctt tttacctgac cggtggaacc gcgcttagta gaggctacta
     2521 taatcatcgt tattcagacg atttagattt ctttttaaat cacgattcaa attttgattc
     2581 ttatgtgagt tctgttctcg aggaactcaa aatcaaaggt ttttattgga gtaacgagat
     2641 aggttttgtt aaatctatgg acttttattc tttaatagtt cagcattcag atttttgcac
     2701 taaattaaaa ttggattttg tcaatgatat cgtcgctcat tttggtgata tacaaaatac
     2761 agacgtatat tatagaactg attcaatacg caacattctt tcaaataaaa ttactgcaat
     2821 atttagaatg tctgccaagg atgttgttga tattcgagaa atctgtatac atgaaaaatt
     2881 tgattggaat gaaattttta cagaagttcg cgagaaagaa ttgggcattg aaccattaga
     2941 agtagtagaa attcttaaag gcattccgaa aacagctttt aatagtatta aatggatcca
     3001 aacgacagat tacgaaacat tcatgtgtga tatagaaatt attgctagag atatgcttgg
     3061 actaactttt aatagtttag taaaatgaaa aaaggataag catattattt ctttgaaatg
     3121 cttgccctgt gataagccca gaagtggtta tcacaatatg caaaaatcct ttattgtatc
     3181 ttcgcagaaa aagtttattg accccttgtt ctgcaatcaa cgccgcacca gtcaatgttg
     3241 aaataataca gccagcatat tttgctcgag ttttatcaaa atgatagtct cggccaccac
     3301 aaaggtgttt agcccggttt actagagttt tagactcatc ttcttttcca tcaggaacgt
     3361 ttagattgat aacaaagccg tcaacacctt ttacttgggg gttgttttta aaaactgtgg
     3421 tggacgaagc tctttttcat caggtttagg tgaacccttc aacgatttta acatttcatt
     3481 aaaagctttc gcatctgttg tatttttagg catcattagc gttctctttt tcggaaaata
     3541 atccgccgaa agaatttagc tttggaatgc caccaagttg tccacttaaa taaagttttg
     3601 tcaaacaatg atcttttcga atatatcctt taggcatttt atattcgtaa agcgctgtta
     3661 aagaggttgc tccgtttcgt tcatttcttt ctgaaatcca tatttcatca cgacgtaaaa
     3721 cttcttgatc catctgcaaa atatcatgat tagtcaaaat taactgtgag cgagtgtctt
     3781 gatttcttgt ctctaaaaaa gaaacaagca gttgcttaac tagattagaa tgcagacttc
     3841 gatctagttc atcaacgaca atgcatacat cagagccttg tgctgttaaa gaatgaaaag
     3901 cagggaataa atgtaaacaa cgcctagaac catctgagtt ttcttttatt tcaaaacgaa
     3961 cttcttcatc attttcgttt ttgtgataag ataccatttt gaaaacagtt aattggctat
     4021 ttcgaaattc aaatttgcaa aaatcatcgg caacattaac tgcccaaacc atcttgtcgc
     4081 cagacgaaag catctgttgt attttttcta gatcttcgct agtaaatcca cttgcttgcg
     4141 gagttatttc ttcaaaatca atacgggaaa ttcctgtatc aattaaactt aatgtatggg
     4201 aaaggttgtg gattggtagt gttttatcgt tgaatctaat gattctactt tctgggctta
     4261 atatcactaa ggaagtacg
//
LOCUS       DAOOBB010000170         4233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_23507_length_4233_cov_4.034466, whole genome shotgun sequence.
ACCESSION   DAOOBB010000170 DAOOBB010000000
VERSION     DAOOBB010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23507_length_4233_cov_4.034466"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="PLT31_07620"
     CDS             <1..837
                     /locus_tag="PLT31_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HPW95036.1"
                     /translation="IGDKKSVEMYKTWYNDVKNALNEHAWDGKWWVRWFDKDGNAYGT
                     NKAKYGKIYCNSQSWPVFSGLADGKRAVQGMDSLEKLLNTENGVKKSTPGYRGFDPTV
                     GGISTYPPGAKENGGIFLHTNPWIMIAETILGRGDKAFRYYCQINPAAKNDKLDEFES
                     EPYCYPQNILGDEHPQFGMGRNAWLSGTSSWTYQAATQYILGVRASFAGLIVDPCIPS
                     TWKGFEMVRRFRGATYNITVKNPKGICKGVEKMVVDGEVIESNVAPIFSSGTHTIEVT
                     LG"
     gene            complement(1005..1970)
                     /gene="holA"
                     /locus_tag="PLT31_07625"
     CDS             complement(1005..1970)
                     /gene="holA"
                     /locus_tag="PLT31_07625"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HPW95037.1"
                     /translation="MILSLIGGDLFSKERRIELFLSESLKDLKDDPLSKQILYATDTN
                     IAIADKVMESCASVSMFSPEQAVVVRRAEALKAADMHTLAEWLNTNPSSSLLFEFEKL
                     AATTDLYKALKKHATIEKYELPRPWEMEKWISNLTVTHFNKAIDPLACRYLSEALGTD
                     TALIAAEIEKILLFAPNCKEISIDLVKTMVVPQREILAFEIRESFGNRDASAFVRKLR
                     ELLDNGVTGIQIASALYHYSVRLLHVNSLLEQGMPPKEIAKKLGANEYLFCIKENEPQ
                     RAKRWNKQLLCRIIKRLADLDFEIKSGQCSSRMSQELALAALIVR"
     gene            complement(1982..2452)
                     /gene="greA"
                     /locus_tag="PLT31_07630"
     CDS             complement(1982..2452)
                     /gene="greA"
                     /locus_tag="PLT31_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HPW95038.1"
                     /translation="MENIPVTQETYDQLVQELENLKKVERPRIITEIADARAQGDLKE
                     NAEYHAAKDRQGEIESRIHYLEDKIARSVIIAYDPSKSETIKFGATVDVKNVKTGKKS
                     TYQLVSPEGVDPINGKISFKSPIGEALLGKTRGEIAEVKLPNGNVNQLEIIDYR"
     gene            2529..3737
                     /gene="argJ"
                     /locus_tag="PLT31_07635"
     CDS             2529..3737
                     /gene="argJ"
                     /locus_tag="PLT31_07635"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HPW95039.1"
                     /translation="MYSIIKDGGVCSPQGFLASGIKAGIKASGNADMALLKSERAARC
                     FAVFTTNKVKAAPVLYDKDVLEHAHFASAVIVNSGNANACTGSQGYADSEKMALLTEE
                     GLNLTPKSVLVCSTGVIGHLMPMDKVEAGIPKLIENLSSDNSIDFAKAILTTDLAMKS
                     LAVEIKTEKGTIRIGGACKGSGMIHPNMATMLAFITTDIGLPIDFFAEFRANIDDSFN
                     AITVDGDTSTNDTCILLANGMSDIQYSDLSLSEQGEFRAALTMLMQELAKSIVRDGEG
                     ATKLIEIRVENAESHKDALKMARFIGTSNLAKCAMFGEDPNWGRILSSAGSSGVNMVA
                     DHTDLYFGDVQVLSNGRPVACNKEALHAVVRQREYKVTLVLNVGPASASAFTCDLSYD
                     YVKINAEYTT"
     gene            complement(3739..>4233)
                     /locus_tag="PLT31_07640"
     CDS             complement(3739..>4233)
                     /locus_tag="PLT31_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HPW95040.1"
                     /translation="EYEWVRIDSSIWTAQNINFAREQGSLCMQCASWGRLYDFEAIQK
                     ACPKGFHIPSEAEWVSLLNFAGKDPGPKLKATFGWDPIGSTGNIKNGTNELGFQIKAG
                     GAHYAPANVPLKERKFDDAGKRAYFWTAEGKVVVFFHEKDNYQIQKWNPIHGASLRCI
                     KDTP"
BASE COUNT         1239 a          797 c          895 g         1302 t
ORIGIN      
        1 atcggcgaca agaaatctgt tgaaatgtac aagacatggt ataacgacgt taaaaacgcg
       61 cttaacgaac atgcttggga tggtaaatgg tgggttcgct ggtttgataa agatggcaat
      121 gcctacggaa ccaacaaagc caagtatggt aagatttatt gtaatagcca atcatggcct
      181 gtatttagcg gcttagccga tggcaaacgt gctgttcaag gcatggacag cttagaaaag
      241 ctcttaaata ccgaaaacgg tgtgaagaaa tccacaccag gttatcgtgg ctttgatcca
      301 acggtgggtg gtatttccac atatcctcct ggagcaaaag aaaacggtgg tattttcttg
      361 catactaatc cttggattat gattgcagaa actattctcg gtcgtggtga taaggctttc
      421 cgttactatt gccagattaa tccagctgct aagaacgaca agttggatga atttgaatcc
      481 gaaccatatt gctacccaca gaacatctta ggtgatgaac atcctcagtt tggtatgggt
      541 cgtaacgcat ggctttcagg tacaagttcc tggacttacc aagctgcaac tcaatacatc
      601 ttgggtgttc gcgctagctt cgctggctta attgtggatc cttgcattcc ttctacttgg
      661 aagggctttg aaatggttcg cagattccgt ggcgctacct acaatatcac tgtaaagaat
      721 cctaagggaa tctgtaaagg tgtagaaaag atggttgtcg acggagaagt cattgaaagt
      781 aatgtggctc ctattttctc tagtggcaca catacaatcg aagtcactct cggttagttt
      841 tatatagaac aaaaaaaaga ctctgcttag gcagggtctt tttttttggg gggaaaatta
      901 aaataccgaa aaaaagtcaa taattaatag ttcgaaaatt ataaagccca aaaaaatagg
      961 catgaaaaaa tcacgcctaa aaataatttg taaaaatgtt cttgttaacg aacaattaga
     1021 gcggcgagag caagctcttg cgacattctc gagctacatt gccctgattt gatttcaaaa
     1081 tcaaggtctg caagcctttt tatgatacgg caaagtaact gcttattcca tcttttggca
     1141 cgttgaggct cattttcttt aatgcagaaa aggtattcat ttgcccctaa tttttttgca
     1201 atttctttgg gaggcatgcc ttgttctaat aagctattta catgtaaaag acgaacagag
     1261 taatggtaca gagcagaagc aatttgaatg cctgtcaccc cattatctaa aagctctctt
     1321 agctttctaa caaaagcaga tgcgtctcga ttgccaaaag attctctaat ttcaaaagca
     1381 agaatttctc tttgagggac caccatcgtt ttgactagat cgatagaaat ttctttacag
     1441 ttaggcgcaa ataaaagaat cttttcaatt tcagcggcga tcaaagcggt atctgttcca
     1501 agtgcttcgg atagataacg gcaggctaga ggatctatag ctttattaaa atgtgtgacg
     1561 gttaagttcg aaatccattt ttccatttcc caaggacgtg gaagctcgta tttctcaatc
     1621 gttgcatgtt tttttaaagc tttgtataaa tccgtggtgg cggctagttt ttcaaattca
     1681 aagagtagag agctagaagg attggtgttt aaccattcag caagagtatg catgtcggct
     1741 gcttttaagg cttccgcacg tcgcacgact accgcttgtt caggagagaa catggagaca
     1801 gacgcacaag attccatcac tttatcagca atggcaatat ttgtgtctgt agcataaagg
     1861 atttgttttg acagaggatc gtcttttaaa tcttttaacg attcagataa aaaaagctct
     1921 atccgccgtt ctttgctaaa taagtcacca ccaatcaatg atagaatcat aggattttta
     1981 attacctgta atctattatt tccagttgat taacgttacc gtttggtaat ttgacttcag
     2041 caatttcacc acgagttttg cctaataaag cttcaccaat agggctctta aaactgattt
     2101 taccattgat aggatctacg ccttctggag aaaccagttg atacgtgctt tttttacctg
     2161 ttttgacgtt tttaacatca accgtagcgc caaatttaat ggtttctgat ttagaggggt
     2221 cataagcaat gatcacggaa cgagcaattt tgtcttcgag atagtgaatt ctagattcaa
     2281 tctctccttg acgatctttt gctgcgtgat attcagcatt ttctttaaga tcaccctgag
     2341 cacgggcatc tgcaatctct gtgatgatac gaggtcgctc tacttttttt aaattctcca
     2401 actcttgtac aagttggtcg tatgtttctt gtgttacagg gatattttcc atactttaca
     2461 aggtacaaat ttttttgaac cattgtttgt tattttaaaa agaaatggac ttttaggaga
     2521 aaaccttcat gtattctatc attaaagatg gcggcgtttg ttctccccaa ggctttttag
     2581 cttcgggaat taaagcagga attaaagcga gtggcaatgc agatatggct ttgctaaaaa
     2641 gtgagcgagc agcacgttgt tttgctgtct ttaccacgaa taaagtgaaa gcagctccag
     2701 ttctttatga caaagatgtt ttagaacatg ctcactttgc ttccgctgtg attgtaaata
     2761 gtggaaatgc gaatgcttgc actggcagtc aaggttatgc tgacagtgaa aaaatggctc
     2821 ttctcactga agagggtttg aatttaacac caaagagcgt tctagtctgt agcacgggcg
     2881 tgattggtca tttaatgcct atggataagg tggaagcggg aattcctaag ctaattgaaa
     2941 atttaagctc agataattct attgattttg caaaagcgat tttaacaaca gacctcgcta
     3001 tgaagtctct cgctgttgaa attaaaacag aaaaaggaac tattcgtatt ggtggagcct
     3061 gtaagggaag cggtatgatt caccctaaca tggcgacgat gcttgctttt attacaacag
     3121 atattggtct tccaattgat ttctttgctg aatttagagc gaatattgat gactccttta
     3181 atgcgattac tgtggatggc gatacaagta caaacgatac ttgcatattg ctcgctaatg
     3241 gtatgagcga tattcaatat tctgacttga gtttatctga acaaggtgaa tttagagcgg
     3301 ctctcaccat gctaatgcaa gagcttgcaa agtctattgt acgcgatgga gaaggcgcta
     3361 caaagcttat tgaaattcgt gtagagaatg cagaatcgca caaagatgcg ttaaaaatgg
     3421 ctcgttttat tggaacaagt aatctcgcta aatgtgctat gtttggagaa gatcctaact
     3481 ggggacgaat cctttcaagt gctggttcta gcggtgtcaa catggttgca gaccatacgg
     3541 atctctattt tggagacgtt caagtgctct ccaatggccg tccagtagca tgcaataaag
     3601 aagcattaca cgctgtcgta agacaacgtg aatacaaagt aacactggtg ttaaatgttg
     3661 gtcctgcttc tgcaagtgcc tttacttgcg acttaagcta tgattacgtt aaaataaatg
     3721 cagaatacac cacctgattt atggtgtatc ttttatacaa cgtaagctag ccccgtgtat
     3781 aggattccac ttctgaattt ggtagttgtc tttttcgtga aagaaaacga caactttacc
     3841 ttcagcggtc caaaagtaag ctctcttccc agcatcatca aattttcttt ctttaagagg
     3901 tacattagca ggagcataat gggcgcctcc tgctttaatc tgaaagccta attcattggt
     3961 gccattttta atatttccag tgcttccaat agggtcccag ccaaaggtgg ctttgagttt
     4021 tggccctgga tctttacctg caaaattcaa aagagaaacc cactctgctt cggaaggaat
     4081 atgaaatcct ttagggcaag ctttttgaat ggcttcaaaa tcatagagac gtccccagct
     4141 agcgcattgc atgcaaagag atccttgttc tctagcgaag ttgatgtttt gagctgtcca
     4201 aatagaagag tctatgcgaa cccattcgta ttc
//
LOCUS       DAOOBB010000171         4221 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_23575_length_4221_cov_3.222036, whole genome shotgun sequence.
ACCESSION   DAOOBB010000171 DAOOBB010000000
VERSION     DAOOBB010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23575_length_4221_cov_3.222036"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1436
                     /locus_tag="PLT31_07645"
     CDS             <1..1436
                     /locus_tag="PLT31_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HPW95041.1"
                     /translation="EKILTIAMSDPTNMRVIDIVKFKAQMDIDVVMASEKDVSAAIER
                     LYAERVSADETLSSLLSASDEKDELETVERDGYNQEPELTDEEGQQVVKIVTTLIHEA
                     IARGASDIHLEPQETYLKLRYRIDGDLQVMAPIPARLMAQILSRIKLLAKMDIAEKRK
                     PLDGRFTVRYRGSEVDLRVSSFPVSLRKRGVAEKIVMRILDPNSGQFPLKDMGFDPRV
                     FKQFLEAINLPNGIILVTGPTGSGKSTTLYASIREILDSTINISTMEDPVELNVDGVN
                     QGQINVAAGFTFAAGIRALLRQDPDVIMIGEMRDRETSSMAIEAALTGHLVFSTLHTN
                     DASGAFPRLLEMDLEPFLVSTAIKGVLAQRLVRRICKYCKEPLDIPQELRDELHLTPE
                     MQFYHGKGCPKCDGSGYKGRCGIYEFLVPNETIRNLILKRASGDDIKRAAIQETNLIT
                     LRMDGIQKALDGLTTLEQALSASTKDD"
     gene            1436..3853
                     /locus_tag="PLT31_07650"
     CDS             1436..3853
                     /locus_tag="PLT31_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPW95042.1"
                     /translation="MSCLVDDVLLKKELNQEQSAAALKIQGPMLILAGAGSGKTRAIT
                     YKMAHLISAHHVDPSHILAVTFTNKAAREMKERIHQILKVPVQLSWMGTFHSICVRIL
                     RLCLSHPASVASLGWPFTSQFSIYDDDDQKRILKEILKNDLGDAYDANELKKVRSIIS
                     KYKNTVRKERLPGGQYKLVLQTPAVVEQNANFADEEKMAGYYKAYQKKLSESNAMDFD
                     DLLLNTVDMLQRLPAVAAQLARQFEYVIVDEYQDTNDVQYELLKLLMNDSHNVTVVGD
                     DDQSIYGWRGANIEIIRNFHRDFHPVTIVKLERNYRSTANIVQGAGSVIANNVRPEEM
                     KKVVFSKEEKGDLIRVSYVDEDRREAEKIARTILNAGESIYSETAIFYRTNAQSRALE
                     KALNDFRIPCVIYGGTRFWDRKEVKDILAYLRLLSNPKDDAALLRVINVPARQIGKTT
                     VVQLMESAENHESSLWEELSVQVEQGSRNASKLKGFKEQVDSWLALVNGKETPLPLIA
                     ERIIEDTLYRNYLKKEDELTADERALNLDEMINAIREFDEENPEATLDSFLQDISLLT
                     DADKKTENSKEKVTLMTMHMAKGLEFHTVHIAGCDEEIFPLVRSTAMMTQEEQKAQME
                     EERRLFYVGCTRAKKKLYLYHAARRFWQGTIRPFPPSRFLAEMDSTVVELKDETSFDL
                     MGSKSFLNSERERAMPTARGFQRSFTYNRPSSSSSKNGGTQRIVYKNPIKVPSEKTEG
                     PRMVYDEYSSESPIRPGVKVRHIRFGVGTLIRSSGQGENTRVDVRFTDGTVRTLVLKF
                     ASLEIMG"
     gene            3904..4191
                     /gene="gatC"
                     /locus_tag="PLT31_07655"
     CDS             3904..4191
                     /gene="gatC"
                     /locus_tag="PLT31_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HPW95043.1"
                     /translation="MLERSDILKLAKLSRLDLSEADMESVSTHLGQMLNHMEALCELD
                     LSGVEPMTGVENETTPLREDVPQPSFSTEQIFRNAPKVESDHFVIPKVIGG"
BASE COUNT         1307 a          729 c          921 g         1264 t
ORIGIN      
        1 aagaaaagat tttaacgata gcgatgtctg atcctacgaa catgcgagtg attgatatcg
       61 ttaagtttaa ggctcaaatg gatatagatg tggtgatggc ttctgaaaaa gatgtttcag
      121 cagctattga acgtctatac gcagaacgag taagtgcaga tgaaacgcta agctcacttt
      181 taagtgcatc tgacgaaaaa gatgaattag aaactgtcga acgagatggc tataatcaag
      241 aaccagagtt gactgatgaa gaaggtcagc aggtggtaaa gattgtaaca accttgattc
      301 atgaagctat tgcacgtgga gcttctgata ttcacttaga gcctcaagaa acgtatttga
      361 aattacgtta ccgtattgat ggtgatcttc aagtgatggc tcctatccct gcgcgactaa
      421 tggctcaaat tctatcaaga attaagctgt tagcaaaaat ggatattgca gaaaaaagaa
      481 agcctttgga tggccgtttt accgtgagat acagaggcag tgaagtggac ttacgtgtga
      541 gctcattccc tgtttcatta cgtaaacgag gtgttgctga aaaaatcgtt atgcgtattt
      601 tagaccctaa ttcaggccaa ttcccgctga aggatatggg gtttgatcca cgtgttttca
      661 aacaattctt agaagccatt aatttaccga acggcattat tcttgtgact ggcccaacag
      721 gatctggtaa atcaaccact ctttatgctt cgattcgcga aattctggat tccacaatta
      781 atatttctac gatggaagac cctgtggaat tgaatgtgga tggcgtgaat caagggcaaa
      841 tcaatgtggc ggctggcttt acttttgctg ctggtattcg agctcttctc cgtcaggatc
      901 cagacgtaat tatgatcggt gaaatgcgcg atagagaaac gtcttccatg gctattgaag
      961 ctgccttgac tggtcactta gtgtttagta cacttcatac gaatgatgct tctggtgctt
     1021 tccctcgttt gcttgaaatg gatcttgaac ctttcttggt ctcaaccgca attaaaggtg
     1081 ttttagcaca acgactagtg cgtcgtatat gtaaatattg caaggaacct ttggatattc
     1141 ctcaagaact tcgtgatgaa cttcatctca ctcctgagat gcaattctat catggaaagg
     1201 gttgcccgaa atgtgatggt tctggatata aaggacgttg cggtatttat gaatttttag
     1261 tccctaatga aactattcgt aacttgattc ttaaaagagc ttcaggtgac gatattaaac
     1321 gtgctgcgat acaggaaacg aatttgatta ctcttcgaat ggatggaatc caaaaagcct
     1381 tagatggtct taccacacta gaacaagctc tttcagcatc gacaaaggat gattgatgtc
     1441 ttgtcttgtg gatgatgttt tattaaaaaa agaattaaac caagaacaat ccgctgcagc
     1501 ccttaagata caagggccaa tgcttatttt ggcgggtgct ggttctggta aaactagagc
     1561 aatcacttat aaaatggctc acttgatttc agcacatcat gtggacccta gtcatattct
     1621 tgcggtcacc ttcactaata aggcggctcg agaaatgaaa gaacgcatac atcaaatatt
     1681 aaaagttcct gtccaattaa gttggatggg gacattccat tcgatttgtg ttcgtatttt
     1741 aagactttgc ttaagtcacc cagcgtcagt agcatcactt ggctggcctt ttacatcgca
     1801 gttttcaatt tatgatgatg atgatcaaaa acgaatttta aaagaaatcc taaaaaacga
     1861 tcttggagat gcctatgatg cgaatgaact aaaaaaagtc cgttcgatta tctctaagta
     1921 taaaaacact gttagaaaag aacgtttgcc tggtgggcaa tataagcttg tattacaaac
     1981 gcctgctgtt gttgaacaaa acgctaattt tgctgatgaa gaaaagatgg ctggttatta
     2041 taaagcatat cagaaaaaac tttctgaatc taatgcaatg gattttgatg atttattgct
     2101 caataccgtc gatatgttac agaggctccc agcagtagcc gctcaattag ctcgtcagtt
     2161 tgaatatgtg attgtggatg aatatcaaga tacaaatgat gttcaatatg agcttttaaa
     2221 attgttaatg aatgattctc ataatgtgac tgtggtgggt gatgatgacc aaagcattta
     2281 tggttggcga ggagcaaaca tcgaaatcat tcgcaatttt catcgagatt ttcatcctgt
     2341 gacgattgtg aagttagaac gaaattatcg ttctacagcc aatattgtgc aaggtgcagg
     2401 ttctgtgatt gcaaacaatg tgcggcctga agaaatgaaa aaagtggttt tttctaaaga
     2461 agaaaaaggc gatttgattc gtgtttctta tgtcgatgaa gaccgacgtg aggcagaaaa
     2521 aatagcacgc accattttaa atgctggaga atcaatttat tctgagaccg ccatttttta
     2581 tcgaacgaat gcacagtcac gagccttaga aaaagcacta aatgatttta gaattccttg
     2641 cgttatttat ggtggcactc gtttctggga tcgaaaagaa gtaaaagata tcttggcata
     2701 cttgcgctta ctatcaaatc caaaagatga tgccgctctt ttaagagtca tcaatgttcc
     2761 tgctcgtcag ataggaaaaa ccacagttgt acaattaatg gaaagtgctg agaatcatga
     2821 atcttctctt tgggaagaat taagtgtaca ggtggaacag ggcagtcgaa atgcttctaa
     2881 attaaaggga tttaaggagc aagttgattc ttggcttgct cttgtgaatg gaaaagaaac
     2941 ccctcttcca ttaattgcag agcgtattat tgaagatact ctttatcgga attacttgaa
     3001 aaaagaagat gagttgactg cagatgaacg agcattaaac ttagatgaaa tgattaatgc
     3061 cattcgagaa tttgatgaag aaaatccaga ggctacatta gattcttttt tacaagacat
     3121 ttctttacta acggatgcag ataaaaagac agagaatagt aaagagaaag taactcttat
     3181 gacgatgcat atggcaaagg gtcttgagtt tcatacggta cacattgctg gttgtgatga
     3241 agaaatattc cctctcgttc gttctacggc gatgatgact caagaagaac aaaaagcaca
     3301 gatggaagaa gaacgtcgtc tgttttatgt cggttgtact cgtgcaaaga aaaagctgta
     3361 tttatatcat gccgctcgtc gtttttggca gggaacaatt cgaccatttc ctccctctcg
     3421 ttttttagca gaaatggatt ctactgtagt agaattgaag gatgaaacat cgtttgattt
     3481 aatgggatct aaatcatttt taaattcaga aagagaaaga gcaatgccaa cagcaagagg
     3541 ctttcagcgc tcttttactt ataatcgccc aagctcatca tcttcaaaaa atggaggcac
     3601 gcagcggatt gtttataaaa atccaattaa agtgccttct gaaaaaacag agggacctcg
     3661 aatggtttat gatgagtata gtagtgagtc gcctattcgt ccaggagtaa aggttcgtca
     3721 cattcgtttt ggtgtcggta ctttaatccg ctctagtgga caaggcgaga atactcgagt
     3781 ggatgttcgt tttacggatg gaaccgtacg cacattagtt ttaaaatttg ccagtttgga
     3841 aataatgggt tagtctaatg atgtttactt ttatatcttt tgaaggattt ttcggaggct
     3901 tatatgcttg aacgtagtga tatcttgaaa ttggcaaaac tctccagatt ggatttatct
     3961 gaagcagata tggaatctgt ttcaacgcat ctaggacaaa tgcttaatca catggaagct
     4021 ctgtgtgaat tagatttaag cggtgtagag ccaatgacag gtgtagaaaa tgaaaccacg
     4081 cctcttcgag aagatgtgcc tcaaccttct ttttctacag aacaaatttt tagaaatgcc
     4141 ccaaaagtgg aaagcgatca ctttgtgata ccaaaagtta ttggcggcta aatgtccgtt
     4201 tcttgtgtca ttcctgcgcg t
//
LOCUS       DAOOBB010000172         4079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_24585_length_4079_cov_4.973410, whole genome shotgun sequence.
ACCESSION   DAOOBB010000172 DAOOBB010000000
VERSION     DAOOBB010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24585_length_4079_cov_4.973410"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..1162
                     /gene="fmt"
                     /locus_tag="PLT31_07660"
     CDS             236..1162
                     /gene="fmt"
                     /locus_tag="PLT31_07660"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPW95044.1"
                     /translation="MKVIFMGTPEFASHFLSYLVQSNHEVLAVVTQPDRPAGRGKSLT
                     PPPVKILAEKLGLPVIQPENLKDPEFESTLLKYNADIFIVVAFSILPRNILGASRFGA
                     LNIHGSLLPAYRGAAPVQWAIANGEPKTGVTIFLLDEKMDHGPILEQREVKIEDDDTA
                     ETILNKMVLPACEGLEDALKKIEAHSSEFMMQNHDLATAAPKLKKEDGLLDFKKSALE
                     LHNRIRAFSPWPGGYSNLQGKKIYFRKTKVLQENFGLAEGSVLIEKDRFIVGTSAGAL
                     EILEIQAEGKKAMPVSDYVRGLHQKEGLVFCS"
     gene            1153..2379
                     /locus_tag="PLT31_07665"
     CDS             1153..2379
                     /locus_tag="PLT31_07665"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPW95045.1"
                     /translation="MQLDERQIAYKALFQWFKKGTFLQGSSLPPFALKIVLGVCRHHL
                     HLEYIIKKNVSKMPEMPVQIILEMGLYQLFFMDGIPDHAAVFTSAALARWNRQSEGSV
                     ALINGVLRQVKRNGLPELPPQKIKRISIEYSVPEWIVRRWFDSYGAMQAEEMAKETIQ
                     EPVQWIRVNTNKITLKKIREDFDLHGDEYAERYLEVDSNIPMKKLLESREFNEGLFSF
                     QNPAAYDVVSLLKVSSGMKVWDACAAPGGKTSLILEMNPSISLVASDISEERVSKMSD
                     LVERQKLDSVHFNVMDAANTTYNQEFDRILLDVPCSNFGVLSRRPEVVYRTTLESLEA
                     LSNMQFEILQGASKALKKNGLLVYATCSQERVETTGVLKRFLAANPDFALEGKSICTG
                     GIKGMDHFFAQALVRQ"
     gene            2379..2960
                     /locus_tag="PLT31_07670"
     CDS             2379..2960
                     /locus_tag="PLT31_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95046.1"
                     /translation="MKNQYFFALILFAFAYGFSAEVETKDYMFSEGYLALEGGEAYPW
                     GIIEDAVQPSLYGQGEFYYRYWENTYGLVQFGYAYFRTQDYFKRFPGVHQFHGRVGLD
                     HQFSWMRPILLGVGFSCIWARSDGGDPEDKGPGVMLTDNESEFGSFIKAKLPILKYEK
                     YAVGLNIYWEHIWTLPEKSNMLWIGLFVQRRMW"
     gene            2960..>4079
                     /locus_tag="PLT31_07675"
     CDS             2960..>4079
                     /locus_tag="PLT31_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95047.1"
                     /translation="MRIFLLSLVLSISLIHAAIPVETEGMEPAISSDGTYLTASPTYF
                     FDRPSLMAGIHSQSELWSPDFLTNRLLVDRYSVLPSWQIQEPSIWMQVGNEVGDMIYH
                     DILTSSEDRPVNRTPMLEAGFRSNSFHGFWATGRFFQVDHFSSNTLRINRKTAGTMEY
                     SLFGANLPGFSTGYAGLGYTKDQTEISILAGKEYLWLFGESGRWISALLSPRIQSQFH
                     YKKLDLSLVYEDVLYINKEKGEEGTRKEWSGSLYFPYTDCCNSNKFKTGAGLAFRFVN
                     DEGDTYFGLENDRAFWAFLQAKYEPIKDFTVSGHAGMNEKDWLIQDSIQYSLKKGTQT
                     HFLFGVQNILATRLNPMGESYEYFDQDTISLSTDGLLQL"
BASE COUNT         1241 a          690 c          841 g         1307 t
ORIGIN      
        1 gtaatagcca tttccgtcga ccatatcatt aatgagtttt aatgaaaaaa ccattgccgt
       61 caacaagagt aacattgagc tttagtgaaa aaaccatttc cgtcaataac agctattttt
      121 aatgaaaaag ccattcgtcc ctgttttaga gagaaagatt tcttagatga gaatcacgcc
      181 tggaaagatt aaaatgattt ttctttgaaa aaaaacttgc taatattaag aatgtatgaa
      241 agtaatattt atggggacgc ccgaatttgc gtcgcatttt ttgtcttatt tagttcaatc
      301 gaatcacgag gtgctcgctg tagtgacaca gccagatcgt cctgcgggta gaggaaaatc
      361 cttaactcct cctcccgtga aaatccttgc tgaaaaactt gggttaccag taatccaacc
      421 agaaaactta aaagatccag aatttgaatc gacgctttta aaatacaatg ccgatatctt
      481 tattgttgtt gcgttttcca ttttaccgag aaatatttta ggcgcgtctc gctttggagc
      541 cttaaatatc catggtagtc ttttgcccgc ataccgcggg gctgctccag tgcaatgggc
      601 tatcgctaat ggtgaaccta aaacgggagt gactattttc ttattagatg aaaaaatgga
      661 tcatggtcct attttagaac aacgagaagt aaaaattgaa gacgatgata ctgcagaaac
      721 tattttgaat aaaatggtgt tgcctgcatg tgaaggttta gaagatgcat tgaaaaaaat
      781 agaagctcat tcttctgaat tcatgatgca aaaccatgac ttagcgactg ccgcaccaaa
      841 acttaaaaaa gaagatgggc ttttagattt taaaaagagt gctttagaat tacataatcg
      901 catacgtgct tttagccctt ggcctggagg ctatagcaac ctacaaggga aaaaaatcta
      961 tttccgaaaa acaaaagtcc ttcaagaaaa ttttgggtta gcggaaggat ctgttcttat
     1021 tgaaaaggat cgctttatag taggcactag tgcaggtgct ttagaaatct tagaaataca
     1081 ggcagaaggt aaaaaagcaa tgcctgtatc tgattatgtt agaggacttc atcaaaaaga
     1141 agggcttgta ttttgcagtt agatgaaaga caaattgcct ataaggcttt atttcaatgg
     1201 ttcaaaaaag gcactttttt acaaggctcc tctcttcctc cgtttgcatt aaaaatagtt
     1261 ttaggagtct gccgtcatca tttacattta gaatacatta ttaaaaaaaa tgtatcgaaa
     1321 atgcctgaga tgcctgttca gattatttta gaaatgggac tttaccagct cttttttatg
     1381 gacggaattc ctgatcatgc tgctgttttt acaagtgcag ctcttgctcg ttggaatcgt
     1441 cagagtgaag gttctgtagc cttaattaat ggtgtattac gtcaagtcaa aagaaacggg
     1501 ctccctgaat tacctcctca gaaaataaag agaattagta ttgaatactc tgttccagaa
     1561 tggattgtac gacgttggtt tgattcctat ggtgcaatgc aagctgaaga aatggctaaa
     1621 gaaaccattc aagaacccgt gcagtggatt cgtgtcaaca ctaataaaat tacacttaag
     1681 aaaatacgtg aagattttga tcttcatgga gatgagtacg cggaacgcta tttagaagta
     1741 gactctaata ttccaatgaa aaaattatta gagtctaggg agtttaatga aggtttattt
     1801 tcctttcaaa acccagcagc ctatgatgtt gtttctttat taaaagtatc ttcaggaatg
     1861 aaagtctggg atgcctgtgc tgcccctggt ggaaaaacct cattaatcct agaaatgaat
     1921 ccatccatct ctcttgtggc ttctgatatt tctgaagaac gcgtttcaaa aatgagtgat
     1981 cttgtggaaa gacaaaaatt ggattctgtt cactttaatg tcatggatgc agcgaacacc
     2041 acctataatc aagaatttga tcgcattcta ttagatgttc cttgttctaa ttttggtgtt
     2101 ctttctcgcc gtccagaagt ggtttaccga accactttag aatcgttgga agccttgtct
     2161 aatatgcaat ttgaaatatt acaaggggct tctaaagcac taaaaaagaa tggcctttta
     2221 gtctatgcta cttgcagtca agaacgagtc gaaactacag gcgtcctaaa acgctttttg
     2281 gctgcaaatc ctgattttgc attggaagga aagtctattt gtactggagg aatcaaaggt
     2341 atggaccatt tctttgctca agcactagtg aggcaataat gaaaaatcag tatttctttg
     2401 cattgatttt atttgcattt gcatacggat tttctgctga agtagaaacc aaagattata
     2461 tgttttctga agggtatctc gctttagaag gtggagaagc ttatccatgg ggaataatag
     2521 aagacgctgt tcaaccctct ttatacggcc aaggagaatt ttactatcgc tattgggaga
     2581 atacttatgg tttagtgcaa tttggatatg cgtattttag aacgcaagat tattttaagc
     2641 gttttcctgg tgttcatcaa ttccatgggc gagttggttt agatcatcaa ttttcttgga
     2701 tgcgtcctat tttattagga gtcggtttta gttgtatttg ggctcgttct gatggaggcg
     2761 atccagaaga caaagggcct ggcgtgatgt tgactgataa tgagtctgaa tttggttctt
     2821 ttattaaagc taagttacct atattgaaat acgaaaaata tgcagtaggt cttaatatct
     2881 attgggaaca tatttggact ttacctgaaa aatcgaatat gctttggata ggtttatttg
     2941 tgcaaaggcg gatgtggtaa tgagaatttt tcttctttct ttagtactta gcatttctct
     3001 gattcatgct gcaattcctg tggaaacgga gggcatggaa cccgctatta gttcagatgg
     3061 aacatattta acggcttctc caacctattt tttcgatcgc ccttctctga tggcagggat
     3121 tcattcgcaa agcgaacttt ggtctccaga ttttttaacc aatagattac ttgttgatcg
     3181 ctattctgta cttccttctt ggcaaattca agagccctcc atttggatgc aagtcggtaa
     3241 tgaagtgggt gatatgattt atcacgatat tctcacgtct agtgaggatc gtcctgtaaa
     3301 tagaacgcca atgcttgaag ctggatttcg aagcaattct tttcatggct tttgggccac
     3361 aggtagattt tttcaagtag atcatttttc ttcaaatacc ctaagaatta atcgtaaaac
     3421 tgctggaact atggaatatt cattgtttgg cgctaacttg cctggtttta gcacaggtta
     3481 tgcgggctta ggttacacta aagatcaaac agaaatatcc attcttgcag gtaaggaata
     3541 tttatggctt tttggagaaa gtggtcgttg gatttctgcg ctcctttcac caagaattca
     3601 gtctcagttt cattataaga agttagatct ttcgttagtt tacgaagacg tcctttatat
     3661 taataaagaa aaaggggaag aaggaacacg aaaagaatgg agtggttcgc tgtattttcc
     3721 ttacacggat tgttgtaatt caaataaatt taaaacaggt gcaggccttg cttttcgatt
     3781 tgtaaacgat gaaggggaca cctattttgg cttagaaaat gatagggctt tttgggcctt
     3841 tctacaagca aagtatgagc cgattaaaga ttttacagtg agtggccatg cagggatgaa
     3901 tgaaaaagac tggcttattc aagatagcat tcaatattca ctcaaaaaag gaacgcaaac
     3961 acattttctt tttggtgttc aaaatatact tgcaacgcgc ttaaatccaa tgggtgaatc
     4021 ctatgagtat tttgatcaag acaccatttc tttatcgaca gatggattat tacaattat
//
LOCUS       DAOOBB010000173         4078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_24598_length_4078_cov_4.215014, whole genome shotgun sequence.
ACCESSION   DAOOBB010000173 DAOOBB010000000
VERSION     DAOOBB010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24598_length_4078_cov_4.215014"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1514)
                     /locus_tag="PLT31_07680"
     CDS             complement(<1..1514)
                     /locus_tag="PLT31_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HPW95048.1"
                     /translation="MHFSKKSINLFSVLIWSLALQSFLLAQEMTRFELEEREPVVEKD
                     SVEKDWWSDDTSEDTLQYHAVDLTYDVTSSTFNLNKNAQLTYRTATLNGDTIWFDQKN
                     SILVASGDPILKEAKNPSLAGYRMKYNMKSKIGEIYYATTYQDNQKLNGMEVRRLPDT
                     RLHIERGDFSTCNDSTHQHFYFYGRRMVVKPKESVTARPIVLNIADVPVAVLPMIVAP
                     LKSGRKSGILTPKFGGDQVQGFYLKNLGYYWAASEYFDATINADIVEGAEARFERTTL
                     NGLVRYKKRYVLDGNISATSYLDEFDFSNSGYDIGFSHNQNLTPDGKHTLSGSGSFVS
                     HQRVRQDNALDEETILDQQANAQMTYAARFGSNKSLTIKLHQQHNLTTDLLERQIPDI
                     QYQMGGPLFSFLKNNDDDEASEESFLDKFNYSFSNRANLYSKRALDSLAQEDTTAWYS
                     GYNGQLVLDYSGSFLDVINVTPRARLGAMWSGRSWINPEDSIIYRKAENALDLG"
     gene            complement(1578..2390)
                     /locus_tag="PLT31_07685"
     CDS             complement(1578..2390)
                     /locus_tag="PLT31_07685"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPW95049.1"
                     /translation="MQFRKILTVLALASVASFASEVFGGIGVTIYGISEGVQVVEVIP
                     GTPAAEAGILPGDKIIAVDQVSLKGLTQDQSIEKLRGEAGRPVEATVLREGEKLSMVM
                     RRAQLTVQDIDAGSLSAWYGNKLNQFDAEEIKVFAESKAKETATLLGVMRQGHLISSE
                     AKVAPDDVVGVFIDQKPVFETPAVSNLTKKASSSLKAFNRKVIGFELFKEGPASIKIL
                     DPNGEEVQVIDHQNGLSGINSVTWDGSKIPSGRYMIRIEQNGAVSGHFAVLR"
     gene            complement(2476..3573)
                     /locus_tag="PLT31_07690"
     CDS             complement(2476..3573)
                     /locus_tag="PLT31_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95050.1"
                     /translation="MRHFVWILAFFTCYVGAVERSLFNNPDISTEYSSLLQEKNARND
                     SLLPALDGDKAFAELLRTIPSLWSLDRMGKDEKVLTRLKAKIIYIDGIREEPFCDLDK
                     SNDNSAGEWNLKIGVDFVSGGSNIVGIHVQQCLNMVRDELGYVVQKGDKVVTVPPKKV
                     LEKIKKSEIPSILDMDLQTKLLKAHESVELDGKCPKDIEAYIILMDVWNQVKDTPMDI
                     VVLNDELNRKRNGGKSVQSCAFSREWNKRYQEEASFECDIDERFCLYRQSEEGVSKWG
                     YHFEENRFEYMNKKFLFWNRNPKSIHKGGTMMDLRIIRLSTSLYLEGYMNEKQEPAAL
                     GLLIIPDGNEDQYYEDEEYEAETETESLDRN"
     gene            complement(3578..>4078)
                     /locus_tag="PLT31_07695"
     CDS             complement(3578..>4078)
                     /locus_tag="PLT31_07695"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPW95051.1"
                     /translation="TLSSNNISMVEMSFLFGSILTVPSSLIVIMFMLLFLIVSLTLFY
                     YRDFLLLSYDPEFAQVKGVRVFPLYLLLIVLITFTVVMAVQAVGLILVIALLTIPAYV
                     AESHVNQLKHMIMISVGLSLILVISGLFLAFKINFTVGPVIILLSVFLYLINLGMMTL
                     QKKWRR"
BASE COUNT         1358 a          848 c          659 g         1213 t
ORIGIN      
        1 tcccctagat ctaacgcatt ttctgcttta cggtaaatga tagaatcttc tggattaatc
       61 caagatcgtc cagaccacat ggctcctaaa cgagctcttg gagtcacatt aatgacatct
      121 aaaaaagagc ccgaataatc taacactaat tgtccgttat aacctgaata ccaagcagta
      181 gtgtcttctt gagctaaaga atctaaagct cgtttcgaat ataaattagc tcgatttgaa
      241 aaagaataat tgaatttatc taaaaaagat tcttcagaag cttcgtcatc atcattattt
      301 tttagaaaag aaaaaagagg accgcccatt tgatattgga tatctgggat ttggcgttct
      361 aaaagatctg tcgtcaaatt atgctgttgg tgcaatttaa ttgtaaggct tttattagaa
      421 ccgaatcgag ccgcataagt catttgagcg ttcgcttgtt gatccaaaat ggtttcttca
      481 tctaatgcat tatcttgacg caccctttgg tgagaaacaa aagagcccga tccacttaaa
      541 gtatgctttc catctggcgt taaattctga ttatgagaaa agccgatatc atagccacta
      601 tttgaaaaat caaattcatc taaataactg gtggccgaaa tatttccatc aagcacgtaa
      661 cgttttttat aacgtacaag cccattcaat gtagttcgtt caaagcgtgc ttctgcacct
      721 tccactatat ctgcattgat tgtggcgtca aaatattcgc ttgctgccca gtaataacct
      781 aagtttttca aataaaaacc ttgaacctgg tctccgccaa atttaggcgt caatatgccc
      841 gattttctcc cactttttaa aggagcaaca atcatcggta aaacagcaac aggaacatca
      901 gcaatattca atacgatagg cctagccgtt acgctttctt ttggtttaac caccatgcga
      961 cggccataga aataaaaatg ttggtgagtg gaatcattac aagtactaaa atcgcctcgt
     1021 tcaatatgaa gacgcgtatc tggcaaacgc ctcacttcca ttccatttaa tttttgattg
     1081 tcttgatagg tggtggcata ataaatttca cctattttcg atttcatatt atacttcatg
     1141 cgataacccg caagcgacgg gtttttagct tcttttagaa ttggatctcc cgaagccaca
     1201 aggatactat ttttctgatc aaaccaaatc gtatcaccat ttaaggtcgc cgtccgatag
     1261 gttaattgag catttttatt taagttgaaa gttgaagaag tgacatcata tgttaaatct
     1321 acggcatgat attgaagggt gtcttcagaa gtatcatcac tccaccaatc tttttccact
     1381 gaatcttttt caacaacagg ttcacgttct tccaattcaa aacgtgtcat ttcttgagct
     1441 aacagaaacg actgtaaagc aagactccaa ataagaaccg aaaaaaggtt gattgatttt
     1501 tttgaaaaat gcacggtgat gtaattatag aaaaagcctc tctaatgaga ggctttacat
     1561 tgaatgaaaa gtgcttgtta gcgtagtaca gcaaaatgac cagagaccgc accattctgc
     1621 tcaatacgaa tcatataacg gccactaggg attttggatc catcccaagt cacactgtta
     1681 ataccagaga gaccattttg atgatcaatc acttgaactt cttcaccatt aggatcgaga
     1741 atcttaatag aagctgggcc ttctttgaaa agttcaaagc cgatgacttt acgattaaag
     1801 gctttcagag aagaagaagc cttctttgtc aaattggaaa ctgctggagt ttcaaaaacg
     1861 ggtttttgat caatgaaaac accaaccaca tcatctggag cgacttttgc ttcgctagaa
     1921 attagatgac cttgacgcat cacgcctaat aacgttgccg tttcttttgc tttagattca
     1981 gcaaagactt ttatttcttc ggcatcaaat tgattaagct tgttcccgta ccatgcggat
     2041 agagaacccg catcaatatc ttgaactgtt aactgagcac gacgcataac catagaaagc
     2101 ttttcacctt cacgtaaaac agtggcttcc actggacgac cagcctctcc acgtaatttt
     2161 tcaatgcttt gatcttgagt gagccctttt aaggaaactt gatctacagc gataatctta
     2221 tcacctggca aaatgccagc ttctgctgct ggggttccag gaataacctc tacgacttga
     2281 actccttcgg agataccgta aatagtgaca ccaataccac caaaaacttc tgatgcaaaa
     2341 gatgcgacag acgctaaagc taagacggtt agaattttac gaaattgcat acatgcctcc
     2401 attctattca gaaaatataa ctatatccct caaaaaacaa ctatgaaact agggattcta
     2461 atgtaaaatg tgcttttagt tgcggtctaa agactccgtt tccgtttcgg cttcatattc
     2521 ctcatcttcg taatactgat cttcatttcc atcgggaata attaaaaggc ctaaagccgc
     2581 aggttcctgt ttttcattca tgtaaccttc caaataaaga gaagtggaaa ggcgaataat
     2641 acgaagatcc atcatggttc ctcctttatg aatgcttttt ggattccgat tccaaaacaa
     2701 gaatttctta ttcatatatt caaaacgatt ttcttcaaaa tggtatcccc atttagaaac
     2761 gccttcttca gattgtctgt aaaggcaaaa ccgttcatca atatcacatt caaaagaggc
     2821 ttcttcttga tatctcttat tccattctcg actaaatgca caagactgaa cagactttcc
     2881 accattgcgt ttacggttta attcatcgtt taaaacgact atatccattg gagtatcttt
     2941 gacttgattc cagacatcca tcaaaataat ataagcctca atatctttag gacacttgcc
     3001 atctaattcc acagactcat gagcttttaa taattttgtt tgtagatcca tatctagaat
     3061 cgatggaatc tcgcttttct taattttttc aagaactttc tttggcggca ccgtcaccac
     3121 cttatcccct ttctgcacca cataaccaag ctcatcacga accatattta agcattgctg
     3181 aacatgtatt cccacaatat tagacccacc agaaacaaag tctacgccta tttttaaatt
     3241 ccattctcca gcagaattat cgttcgattt atctagatca cagaaaggct cttctcgaat
     3301 accatcgata taaataatct ttgcttttaa gcgagttaaa actttttcat cttttcccat
     3361 gcgatctaaa ctccacaaac taggaatcgt tcgcaaaagt tctgcaaagg ctttatctcc
     3421 atctaaagca ggtaataaag aatcattacg tgcattcttt tcttgtaaaa gagaagagta
     3481 ttctgtagaa atatcaggat tattaaataa agaacgctct acagctccca cataacacgt
     3541 aaaaaaagct aaaatccata caaaatgacg catactttta acgtctccat tttttttgca
     3601 aagtcatcat accaaggttt attaaataca aaaatacact caataaaata atgacagggc
     3661 ctacagtaaa gttgatttta aaagcaagaa acaagccgct gatcacaaga attaaagata
     3721 aaccgactga aatcataatc atgtgtttta attgattaac atgagattct gctacataag
     3781 caggaatcgt aagaagagca atgactaaaa tcaagccaac cgcttgcacc gccatcacga
     3841 cagtaaatgt gattaaaaca attaaaagca agtaaagagg aaatacacgc acccctttca
     3901 cttgagcaaa ttcaggatca taagataaaa gtaaaaagtc acgataataa aataaagtaa
     3961 gagagacaat caagaaaagt aacataaaca ttataacaat aagagaggaa ggcacagtta
     4021 aaatacttcc aaataaaaaa ctcatttcaa ccattgaaat attattagac gataaagt
//
LOCUS       DAOOBB010000174         4077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_24607_length_4077_cov_6.513426, whole genome shotgun sequence.
ACCESSION   DAOOBB010000174 DAOOBB010000000
VERSION     DAOOBB010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24607_length_4077_cov_6.513426"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1392
                     /locus_tag="PLT31_07700"
     CDS             <1..1392
                     /locus_tag="PLT31_07700"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HPW95052.1"
                     /translation="KMMAHSSVSDLESEFGIWFKSVRIALDLFKTENIPLETAVHQEV
                     QAYQKITGAMSVDYEGKTYTLPQMAPFLESPNRNLRQKVWELTSERRLLDKESLDLAF
                     DKLFKLRMQISKNTGSSDYLDYIFKAKGRFDYTPSDCRNFHTSIEKIILPALREIYKK
                     RKDDLGVSTLRPWDLQCDPKSLKPLKPFENGKDLIQKCNLFFKKLHPKTAEWFQIMVD
                     QNLIDPDSRLGKAPGGYQIGFDESRLPFIFMNAAGTDRDMYTLFHEGGHSFHQFAMAD
                     QPIIAYRDIPSEFAEVASMSMELIATSDLSAFYSKEDAKRSIFGQLEDVIWLFPWVAS
                     IDSFQHELYSRPNHTAEDRKNIWLSIMDRFDSGVDYSGYDDVKAYLWQKQLHLFEVPF
                     YYIEYGIAQLGALQVWANFKKDPEKALDQLFKAESLGNSVSLPELFKQAGIRFDFSPS
                     TIEPLIENVLEKL"
     gene            1455..1539
                     /locus_tag="PLT31_07705"
     tRNA            1455..1539
                     /locus_tag="PLT31_07705"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1489..1491,aa:Ser,seq:gga)
     gene            1567..1884
                     /locus_tag="PLT31_07710"
     CDS             1567..1884
                     /locus_tag="PLT31_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95053.1"
                     /translation="MSDQRVYLDDIYASEKCGGSVFRAVVMMAHEARFINEQARLGYL
                     QLTKKPTTIAMYKFKEDKLSINEKVDEPEVTLEADSLAPATSDIDLSSEKVDEAADAA
                     FGE"
     gene            complement(1992..2732)
                     /locus_tag="PLT31_07715"
     CDS             complement(1992..2732)
                     /locus_tag="PLT31_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95054.1"
                     /translation="MSTHCVIARRLAKNVIQYGCIICDGDLDAVGLRLIRWYNTPKRV
                     EYLFSLGQLESLGGPGSENSGGIMATRKINPPCQHKICESENEMRPDIDFIDYYYIYE
                     SDGKWYYDNPDSVCKVPLLYSLYRLERLRNHPEELPKKYQSDRSDFPFRQENDRILLK
                     YVFYEIPKIDPEFKSLLESKQINVDDVYKQLCEMDFPIAKMNNDLKQIFRYFYPHFVF
                     KTDSDGDRIVKILHRKVSEPRLETIEWE"
     gene            complement(3125..>4077)
                     /locus_tag="PLT31_07720"
     CDS             complement(3125..>4077)
                     /locus_tag="PLT31_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HPW95055.1"
                     /translation="LFAREERMNRSQFIAMAKDVKANPAKLAEYRKVFKNVYPFSDGI
                     FKMLMANEAKPERTIKFLNAMLGLEGSEAITEFTLGVQENPGILNDKTAIFDVYGITQ
                     SGEPVLIEVQQNADKLFADRLIYYTSRVVSRTVKASQKYELPHIYVLSILTEDLFILE
                     KDTYFHRTQIVRNKNHFYDKLDVYFVELEKFFNIEDRTPNENREKSKRADMLRLFRAV
                     LEDDDISEDRLRGLLDKDFVKDVSLKGYSDELLLNEVDEMTNIAYEKESSYLEGKDDG
                     IKQGIEQNKREMAKSLKEQGFPISAIEKASGLSEEEIRSL"
BASE COUNT         1199 a          790 c          738 g         1350 t
ORIGIN      
        1 aaaatgatgg ctcactcatc tgtttctgac ttggaatctg aatttggaat ttggtttaaa
       61 tcggttcgaa ttgccttaga tctatttaaa acagaaaata ttcctcttga aactgccgtt
      121 catcaagaag tgcaggccta tcaaaaaatc acaggggcca tgagtgtgga ttatgagggt
      181 aaaacataca cattgcctca aatggctcca tttttagaat ctcctaatcg aaatcttcgc
      241 caaaaagttt gggaattaac ttctgaaagg cgtttgttag ataaagaaag tttagacctt
      301 gcttttgata agctatttaa gcttcgaatg caaatttcga aaaatacagg ctcttcagat
      361 tacttggatt atatttttaa ggcgaaagga cgttttgatt atacaccaag cgattgtaga
      421 aattttcaca ccagtattga aaaaatcatt ttgcctgctt tacgtgaaat ctataaaaag
      481 cgcaaagacg atttgggagt aagcactctt cgtccttggg atctccaatg tgatccaaaa
      541 tctctaaaac ccctaaaacc attcgaaaat ggcaaagatt taattcaaaa atgtaatttg
      601 ttttttaaga aactacaccc taaaacagca gaatggtttc agattatggt cgatcaaaac
      661 ttaattgacc ctgatagtcg tttaggaaaa gctcctggag gttatcaaat tggatttgat
      721 gaaagccgtc ttccatttat ctttatgaat gccgctggta cagatagaga catgtacacc
      781 ttattccatg aaggcgggca ctctttccat caatttgcaa tggcagacca acccattatc
      841 gcctatcgag atatcccttc tgaatttgca gaagtcgcga gcatgagtat ggagttaatc
      901 gccacttctg atttaagtgc tttctattca aaagaagatg ccaaaagaag catttttggg
      961 caattagaag acgttatttg gctatttcct tgggtcgcga gtattgatag cttccaacat
     1021 gagctgtatt caagaccaaa tcacactgca gaagaccgca aaaacatctg gctttcaatc
     1081 atggatcgct ttgattcagg cgttgattat tctggctacg atgatgtaaa ggcttatttg
     1141 tggcaaaaac aacttcatct ttttgaagtt cccttctact atattgaata tggtattgct
     1201 caattaggtg cgcttcaagt ttgggcaaat ttcaaaaaag acccagaaaa agctttagat
     1261 caattattta aggcagagtc tctaggaaat agcgtgtctt tacctgaatt attcaagcaa
     1321 gctggtattc gttttgactt ctccccaagc acaatagaac ctttaattga aaatgtgctt
     1381 gaaaaattat aataattgat tcatcatccc ttgctctatt tacgtttttt tattataatt
     1441 gggccacatc ctatggaggg atggccgagt ggtttaaggc gcacgcttgg aaagcgtgtt
     1501 tactttgctg taacgagggt tcgaatccct ctccctcttc taaaactttt ctctagaggt
     1561 aatcatatgt ccgatcaaag agtctatcta gatgatattt atgctagcga gaaatgtggc
     1621 ggttctgtat tcagagctgt tgtcatgatg gcacatgaag ctcgattcat caacgaacaa
     1681 gctcgtctag gttatcttca attgacaaaa aagccgacta cgattgccat gtataagttc
     1741 aaagaagata aattgagcat taatgaaaaa gtcgacgaac cagaagtaac tctcgaagcc
     1801 gattctttag ctccagccac ttctgatatt gatctctctt ctgaaaaagt agacgaagcc
     1861 gcagacgctg cttttggtga ataatcttta ttcttaaaga aattaaaaaa agactctttt
     1921 cgagtctttt ttttgtttta aagacggttt gatctttgaa aaatgggcaa aacgtcaaaa
     1981 tttgccctac cctattccca ttcaattgtt tctaatctag gttcagatac ctttctgtgg
     2041 agtatcttta caattctatc accatctgaa tccgttttga agacaaaatg aggataaaaa
     2101 tatctaaaaa tttgtttcaa atcattattc attttggcaa tgggaaagtc catttcacac
     2161 agttgtttgt aaacatcgtc cacattaatt tgtttcgatt ctaacaatga cttgaattcg
     2221 ggatcaattt ttggaatttc ataaaagacg tatttcaaga gaattcgatc attttcctgc
     2281 cgaaacggaa aatcagaacg atcactttga tattttttag gcaattcctc tggatgattt
     2341 cttagacgct ctagtctata gagcgaatat aacaagggaa ctttacaaac gctatctggg
     2401 ttgtcgtaat accattttcc gtcgctttcg tatatgtaat agtagtcaat aaaatctata
     2461 tctggacgca tctcgttttc cgattcgcaa attttatgtt ggcaaggtgg gtttattttt
     2521 cttgttgcca taattcctcc agaattttcg cttcctggcc cccctaaact ttcgagttgt
     2581 cctagcgaga aaaggtattc tacccttttt ggcgtattgt accaacgaat tagtcgcaaa
     2641 ccaacagcat ccaaatctcc gtcgcagatg atgcacccgt attggattac atttttagcc
     2701 aaacgccttg caataacgca atgtgtactc attttcttct ccttaaaatt cttgtagatg
     2761 aattcaaatt gattttacct aaatgaagaa aaaaaatcat tattctagat tcgtgaatat
     2821 gattacataa ttaccgtgaa aaacacgata ttaatgttcg ctaattaggt tgttgttaag
     2881 tgctctacgt taagcaagcc gtggtccgcg ccactgggga gctgctccgc gaattctttt
     2941 cgctcggcgg caagtcaagc agagagttaa tgcgcccact ctaaagaggg attagaaatc
     3001 actttgtgaa tatatacaaa ttaatattca atatcaatag ctgataaatt tattccttgt
     3061 tcggccttat taactggaat tttatcggtc gttttcaaaa ttttagattc ggtgacatac
     3121 tttattataa gcttctaatt tcttcttcgg aaagacctga tgctttttca atcgcactta
     3181 tcgggaaacc ttgctcttta agtgatttcg ccatttcgcg cttattttgc tcaatacctt
     3241 gctttattcc gtcgtctttc ccttcgaggt atgagctttc tttctcgtat gcaatgtttg
     3301 tcatttcatc tacctcattt aataagagtt catcggagta cccttttaaa gatacatctt
     3361 tcacaaagtc tttgtcaaga agacctctta ggcggtcttc tgaaatatcg tcgtcttcaa
     3421 ggacagcccg aaaaaggcgg agcatatcgg ctcttttgga cttttcacga ttctcgtttg
     3481 gcgttctatc ttcgatattg aagaactttt cgagttctac aaaatacacg tctaacttat
     3541 catagaaatg atttttattt ctaacgattt gcgttcggtg aaaatacgtg tctttttcga
     3601 ggataaatag atcttctgtt aatattgaaa gaacataaat atgcggtaat tcatattttt
     3661 gagacgcctt caccgttctc gaaacgactc tcgatgtata ataaataagg cgatcggcaa
     3721 atagtttgtc tgcattttgc tgaacctcaa tcaaaacggg ttcacccgac tgagttatac
     3781 cataaacatc aaaaatcgct gttttatcat tcaaaattcc aggattttct tgcaccccaa
     3841 gcgtaaattc tgtgattgct tctgaaccct cgagaccgag catcgcattc aaaaatttga
     3901 ttgtgcgttc tggtttagct tcatttgcca ttagcatttt gaaaataccg tcgctgaatg
     3961 ggtaaacgtt cttgaagact tttcggtatt cggcaagttt agctgggtta gccttcacat
     4021 ctttagccat agctatgaat tgacttctat tcattctttc ctcccttgcg aacaagc
//
LOCUS       DAOOBB010000175         4013 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_25104_length_4013_cov_4.118999, whole genome shotgun sequence.
ACCESSION   DAOOBB010000175 DAOOBB010000000
VERSION     DAOOBB010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25104_length_4013_cov_4.118999"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PLT31_07725"
     CDS             complement(<1..259)
                     /locus_tag="PLT31_07725"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95056.1"
                     /translation="MIRSILCISLLLLLSSCEGPRQETFHANGKVKSTVPLVNEKKHG
                     VEKTFYSNGNIQSEVTYNNGLKEGAATEYYEDSTIQAKYTHV"
     gene            complement(284..1381)
                     /locus_tag="PLT31_07730"
     CDS             complement(284..1381)
                     /locus_tag="PLT31_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HPW95057.1"
                     /translation="MRNIFSELGKSDSEIKHKLDTAFQSLFYGNTSTQKIYFETSNDL
                     AYIVDIGHNDIRSEGMSYGMFVTALLDKQDEFEKLWNFSKLYMQHASGEAKSYFSWQV
                     STADFSMMDPGAAPDGEEYIAAALLIAARRFNRKDYQDEAISLINAMAHKEASSEIEP
                     MIDRKHGIVRFSPVTGNDFTDPSYHTLAFYQWFAEATDRSFWESVLNKSRLYLKSAQN
                     SISGLYPDYSEFDGTPKKTAWHPVSHCFSGDAWRVIMNIAIDYALNKHDPSQQESILK
                     TLSFFEERRPYLADYSIDGGLFPTPPREATVGLIAMNGAATIALPPGHPYISSFATDL
                     WNTEPPTGTWRYYDGLLYMIGLLACSGKIEY"
     gene            complement(1398..1946)
                     /locus_tag="PLT31_07735"
     CDS             complement(1398..1946)
                     /locus_tag="PLT31_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HPW95058.1"
                     /translation="MILRPNMNWFANQHGELPSVQVYVQHDEEAIQVRFNVEEPLTCY
                     RAEVQGDGGRCYEDSCVEVFIKALDNPNEYFNFEFNSKAFCLAAKGPSRSERVPFDSS
                     LYSKIHRVMIYPQIQGDFIYWALKVKIPFSILGKSKDSVSKIEGNIYKCADLADKPHY
                     LSLYPVDTEKPDFHRPEFFRSL"
     gene            2095..2448
                     /locus_tag="PLT31_07740"
     CDS             2095..2448
                     /locus_tag="PLT31_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVG family protein"
                     /protein_id="HPW95059.1"
                     /translation="MAKTKAEKKEMMIVKESEPHQVVQFDCLAVTNVNVFPFKEGPTL
                     GKTKAIASVVLNDQLQLTNLRVMDGVNGLFVSYPNDPFYKGEDFRTIYHPVTRQCREH
                     IENCVLEKYQEAISA"
     gene            2455..3978
                     /locus_tag="PLT31_07745"
     CDS             2455..3978
                     /locus_tag="PLT31_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HPW95060.1"
                     /translation="MFQRIRSYCLSGIHAIPVSVEVDSSLGLPGFTLVGLPDSAVRES
                     RERVVSAVRASGYSVTGTRTTVNLSPADLRKEGSSLDLPLAIGMLLASGEFEVNQIDR
                     FVFLGELSLDGLLKPVRGVLSVAMSLVEEKDSVLVVPKDNIQEASLVEGLNVLGVSSL
                     LECIEELKKDCSSLLIRSQGFSSHLKKNHPSYPDFKNIIGMEGVKRALEIAASGNHNF
                     LLVGSPGSGKTLCAKCLPSILPEMTEDEIIESTRIHSCAKSIHSQNFSFIAERPFRSP
                     HHSSTMVSLVGGGHQLLPGEVSLAHNGVLFLDELPEFNRQVLEALREPMEEGSVCIHR
                     ASGSIEWPARFMMGAAMNPCPCGYSMDLKKECTCLPDMKVRYWSKISGPLLDRIDLQI
                     SVPAMDITRLKSTSGLESSATIKKRVKVARTFQEDRFKNSKILYNSEMTSLQTREEAK
                     MDSEAEHFAITAAEKMCLSARGYYRVLKVARTIADLRSSKNVEKLDLAEAFQYRLLK"
BASE COUNT         1239 a          717 c          819 g         1238 t
ORIGIN      
        1 ctacatgcgt gtattttgct tgaatggtgc tgtcttcata atactctgtc gcagcccctt
       61 cttttaagcc gttgttataa gtcacttcgg attgtatgtt tccattagag tagaatgttt
      121 tttcaactcc gtgtttcttt tcattcacaa gaggaacggt gcttttcact tttccattcg
      181 catgaaaggt ttcttgacga ggcccttcac aagaagaaag taaaagtaaa agagagatgc
      241 atagaatgga acgaatcata taactccttt gattaaaaaa tttttagtat tcaatttttc
      301 cagaacaagc gagtaaccct atcatataaa gcaagccatc ataatagcgc catgttccag
      361 tagggggttc tgtattccat aaatcagtcg caaagctcga tatataaggg tgcccaggag
      421 gcaaagcgat tgttgctgcc ccgttcatcg caataaggcc aacagtcgcc tctcttggtg
      481 gagttggaaa tagcccacca tcaatactat agtctgctaa ataaggcctt ctctcttcga
      541 aaaaagagag cgttttaagg atgctttcct gttgagatgg atcgtgctta tttaacgcat
      601 aatctatcgc tatattcata atcacacgcc aagcatcgcc actaaaacaa tggctcacgg
      661 ggtgccaagc tgtcttttta ggagtcccat caaactcaga ataatcagga tataatccag
      721 aaatagaatt ttgagcagac tttaaataaa gacgactctt attcaaaaca gattcccaaa
      781 aggagcgatc ggttgcttct gcaaaccatt gatagaaggc gagagtatga taactcgggt
      841 ctgtaaaatc atttccagtc actggagaaa atcgaacaat gccatgcttt ctatcaatca
      901 taggttctat ttcagaggaa gcctctttat gagccatggc gtttattaaa gagatggctt
      961 cgtcttgata atctttacga ttaaagcgtc tggcggcaat caataaagca gcggcaatat
     1021 attcctctcc atcgggagcg gctcctggat ccatcatgga aaagtcggct gtggaaactt
     1081 gccaagaaaa ataagatttg gcttcaccac tggcatgttg catgtagagt ttagaaaaat
     1141 tccataactt ttcaaattca tcttgtttat ctaaaagagc ggtgacaaac ataccatagc
     1201 tcattccctc ggaacgaata tcattatgcc caatatctac aatataagcg agatcatttg
     1261 atgtttcgaa gtaaattttt tgagtgctcg tattgccata aaatagagat tgaaaagcgg
     1321 tatctagttt atgtttaatc tcagaatcgc ttttaccaag ttcggaaaaa atatttctca
     1381 taataaagga agagtgctta caaggaacgg aaaaattcag gacgatgaaa atccggcttt
     1441 tctgtatcca cggggtagag gcttaaataa tgaggcttat cggctaaatc agcgcattta
     1501 tagatattgc cttctatttt agaaacagag tctttagatt tccctaaaat agagaaagga
     1561 atctttactt ttagcgccca ataaataaag tctccttgaa tctgagggta aatcataact
     1621 cggtgaattt ttgaataaag agaggaatca aaagggacgc gttccgatct cgaagggcct
     1681 ttcgcagcaa ggcaaaaagc tttgctatta aattcaaaat taaaatactc attagggttg
     1741 tctagagctt taataaaaac ttctacacaa ctatcttcgt agcatcgccc gccatcgccc
     1801 tgaacttcag ctctataaca ggtaagaggc tcttctacat taaaacggac ttggatcgct
     1861 tcttcatcat gttgaacgta aacttgaaca gaaggaagtt ccccatgctg atttgcaaac
     1921 caattcatat taggacgaag aatcatgttc taaatatagc gattaatcac ttcattaatc
     1981 agttaatcaa atgaaaagtc tttaaaaaac ttcttttcct tcttaaaaag aacaatttgc
     2041 ttttggcatt cattttgctc tcttaaaatt gtcgtattaa cataggagaa aaatatggct
     2101 aagactaaag cagaaaaaaa agaaatgatg atcgtgaaag aatcagaacc gcatcaagtt
     2161 gttcagtttg attgtttagc tgtcacgaat gtaaacgtct ttcctttcaa agaagggccc
     2221 actttaggaa aaacaaaagc gattgcttct gttgtcttaa atgatcaatt gcagctcacc
     2281 aatttacgcg tgatggatgg tgtaaatggt ctttttgtaa gttatcctaa tgatcctttt
     2341 tataaaggag aggatttccg aacaatttat catcctgtaa caaggcagtg tcgagagcat
     2401 attgagaact gcgttttaga aaaatatcaa gaagctattt cagcgtaaaa caaaatgttt
     2461 caaagaatca gatcttattg cctttctggt attcacgcta ttcctgtgag tgtggaagta
     2521 gactcttcat taggtcttcc ggggtttaca ttggtgggct taccagatag tgctgtaaga
     2581 gaatccaggg aacgtgtggt ttctgctgtc cgcgcttctg gttatagtgt gacaggaacc
     2641 cgtacgacgg tcaatttatc accagcagac cttcgtaagg aggggagttc cctggattta
     2701 cctttggcta taggtatgtt gttagcgagt ggtgaatttg aagtcaatca aatcgatcgt
     2761 ttcgtattcc ttggggagct ttccttggat ggtttattaa aaccagtccg aggagtgctt
     2821 tctgtagcga tgagtttagt agaagaaaaa gattcagtct tagtcgttcc caaagataat
     2881 attcaagaag cttctttagt cgaaggctta aatgttttag gcgtatcgag tttattagag
     2941 tgtattgagg aattaaaaaa agattgttct tctcttttaa tacgctctca aggcttttca
     3001 tctcatttga aaaagaatca cccctcttat cctgatttta aaaatattat cgggatggaa
     3061 ggtgttaaaa gagctttaga aattgcggct tcagggaatc ataatttttt attagttggg
     3121 tctcctggtt ctggaaaaac tctttgcgcg aagtgtttgc caagtatcct tcccgaaatg
     3181 actgaagatg aaattattga aagtacgcgc attcattctt gtgcaaaatc cattcattct
     3241 caaaattttt cctttattgc agaacgtcct tttcgatctc ctcatcattc ttcgactatg
     3301 gtttcattag tcggaggtgg tcatcaatta ttaccaggag aagtgagctt agctcataat
     3361 ggggttctgt ttctagatga acttcctgaa tttaatcgtc aggttttaga agcattacga
     3421 gagccaatgg aagaaggctc tgtatgcatt catagagctt ctggctctat tgaatggcct
     3481 gctcgtttta tgatgggggc tgcaatgaat ccttgtccat gcggttattc tatggatcta
     3541 aaaaaagagt gcacttgtct tcccgatatg aaagttcggt attggtctaa aatttcaggc
     3601 ccgcttttag atcgcattga tttacaaatt agtgttcctg caatggatat tacgcgatta
     3661 aagtctactt cagggttaga gtcatctgcg actattaaaa agcgagtaaa ggtggcgaga
     3721 acgtttcaag aggatcggtt taagaacagt aaaattcttt ataattctga aatgacaagc
     3781 ttacaaacga gagaagaagc aaagatggat tcagaggcag aacattttgc aattacagca
     3841 gcagaaaaaa tgtgtcttag tgcaagaggg tattatcgtg ttttaaaagt ggctagaacc
     3901 attgctgatt taaggagttc taagaatgtg gaaaaattag atcttgcgga agcttttcaa
     3961 tacaggttat tgaaatagca gaaaaaatat aaagctgatt atttttttga aga
//
LOCUS       DAOOBB010000176         3945 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_25662_length_3945_cov_4.487147, whole genome shotgun sequence.
ACCESSION   DAOOBB010000176 DAOOBB010000000
VERSION     DAOOBB010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25662_length_3945_cov_4.487147"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PLT31_07750"
     CDS             complement(<1..414)
                     /locus_tag="PLT31_07750"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820331.1"
                     /note="catalyzes a two-step reaction, first charging a
                     tryptophan molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPW95061.1"
                     /translation="MKPISLTGIKPTGTPHLGNYLGAIRPALDLVKNYETVYFIADYH
                     ALTTVQDGTTMRENIYKVAATWLALGLNPEEGLFYKQSDIPEIFELSWALSCFTPKGF
                     MNRAHAYKDKVAKNLALGEDSDSNVNMGLYCYPCLM"
     gene            486..1616
                     /locus_tag="PLT31_07755"
     CDS             486..1616
                     /locus_tag="PLT31_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HPW95062.1"
                     /translation="MSFEHPYTIVDNESSLQALLRELEFYDIAAVDTEADPMFHYAVS
                     LCLVQITIGDSHWLVDPLANIDLSPIFKTRAMQHIILHGADYDLRLLWQRYHYSPRSV
                     FDTMLAAKFVGEEKLGLADLVYKYFQQSLEKGNQKADWTKRPLPEDMCCYAMHDTTYL
                     HELCALLGEQLLEKKRFSWLTETCSELIEHAKIKAPLKKDPWRMNGSSRLSAKSLNIL
                     KHLWEWREKEAEEMDRPPYKVLSPDLMFMIVKQLSSLSTFDAEALPKLPRNFSGKRIE
                     SFYAMLKNAFQEPVENWPEVYSRPAPPLIPPNAELLEFLKSWRDEKASSLSIDPSMLA
                     NRIQLIALATPGTTSWEERFDNAHFMVWQKNIWIDILQQNLN"
     gene            1630..1824
                     /locus_tag="PLT31_07760"
     CDS             1630..1824
                     /locus_tag="PLT31_07760"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HPW95063.1"
                     /translation="MFRNIGLPEILLLVLLFIVVFGSKRIPDIAESIGKALKKFRKAS
                     QETENDSDSTESENKDDESK"
     gene            1808..2524
                     /gene="tatC"
                     /locus_tag="PLT31_07765"
     CDS             1808..2524
                     /gene="tatC"
                     /locus_tag="PLT31_07765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HPW95064.1"
                     /translation="MTSLNDEMPILKHFQELRSRLLICLVFFIICFLISFYFSHLIWF
                     LLSLPIKEISRLELINLAPAEALEVDFKMSSIAAFILAAPICFYQFYAFCAEALYPTE
                     KRIIITLTLTSTLCFLIGVSFAFFTVLPLLFHFLAEYNAEAKALWSQNAYISFLIRLE
                     LAFGIIFQMPVIAAFLSYFKIIKSNFFIQHFRIAIFVLAFISALITPPDLLSMIFMMG
                     PMLLLYLITIIVNRIFGKGA"
     gene            2524..3213
                     /locus_tag="PLT31_07770"
     CDS             2524..3213
                     /locus_tag="PLT31_07770"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HPW95065.1"
                     /translation="MDLGIGFSEFLLLAILALLFLKPDQMVYTLKKWKTLKGKYFKLK
                     YDIEDHFSAALQKGTHSMKTEQSRWIINETLKLEAFQKANIIAAFFPLPNEPQIEPLL
                     KEIMAMKTLLLPRVSSETEMDFVMISDLEKDLVKGAFGILEPASNLSPWNKSIDLFIV
                     PGVRFSRDGGRIGHGKGYYDRFLSKYPHAYKIGVCFSSQIENKPLSLQPHDVKMNYIV
                     APDNSTKDLFI"
     gene            3210..>3945
                     /locus_tag="PLT31_07775"
     CDS             3210..>3945
                     /locus_tag="PLT31_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPW95066.1"
                     /translation="MNFKENPSSFGRSAHFRNTAPRDSIMRRPRAEDVSIAMSDAETA
                     PQVAVGGMKEVEELITKEPYKVHRVLFLHQSKNPRLYTLQKMARKNHIHVQQVEARIL
                     DSYARPNQGIVALCNEKSLLAWEDIAEEFIQAKEKDVQKIIAVGTNIEDPRNLGACIR
                     SSLALGVDLLLIPAKGMCGITPTVARTSAGALEKLRICRPNNLEGVIAQLKEAGYQIV
                     GLDAATDINLAGFDFEKQIVIAVGGED"
BASE COUNT         1183 a          805 c          706 g         1251 t
ORIGIN      
        1 cattaaacat gggtagcaat aaagccccat atttacattg ctatctgagt cttcccctag
       61 agctaggttt ttagccactt tatctttata ggcgtgagct cgattcataa agccttttgg
      121 cgtaaaacag cttaaggccc aactgagttc aaaaatttct ggaatgtcac tttgtttata
      181 gaaaaggcct tcttctggat tcaaacctaa agcaagccaa gtcgcagcga ctttataaat
      241 attttcgcgc atggtagtgc cgtcttgcac tgtcgttaaa gcatgatagt cagcaataaa
      301 gtagacggtc tcataatttt tcactaaatc gagagctgga cgtatggcgc ccaaataatt
      361 accaagatga ggagtgcccg taggcttaat accagttaaa gaaataggtt tcataataca
      421 aatttaatat ttgaagcacc ttctctgttt tgagataaca agttttttac tagattcttg
      481 ctattgtgag ttttgaacat ccatatacta tagtcgataa tgaatcttct ctgcaagccc
      541 tgttgcgtga acttgaattt tatgatattg ccgccgtcga taccgaagca gatcctatgt
      601 ttcattatgc ggtcagtctt tgcttagttc aaattacgat aggggattct cattggctag
      661 tagacccatt agcaaatatc gatctctccc ctatttttaa aacaagagcc atgcagcata
      721 ttattctaca tggagcagac tacgatttgc gccttttatg gcagaggtat cactattctc
      781 caaggtccgt ttttgataca atgcttgctg ccaaatttgt gggtgaagaa aagcttggat
      841 tagcagacct tgtttataaa tacttccagc agtctctcga aaaagggaat caaaaagccg
      901 attggacaaa acgtccttta ccagaagaca tgtgctgcta cgccatgcat gatacaactt
      961 atttgcatga gctttgtgct cttctcggag aacaattact tgaaaaaaaa cgtttttcct
     1021 ggcttactga aacgtgttct gaattaattg aacatgctaa aataaaagct cctctcaaaa
     1081 aagacccctg gcgaatgaat ggttctagcc gtctttcggc caaaagtctg aacatattaa
     1141 aacacctctg ggaatggcga gaaaaagaag ctgaagagat ggatcgccct ccttataagg
     1201 tactttctcc agaccttatg tttatgatcg taaaacagct ttcctctctt tctacttttg
     1261 atgcagaagc tctgccaaaa ctgcctagaa actttagtgg aaaacgcata gaaagttttt
     1321 atgcgatgtt aaagaacgct tttcaagaac ccgttgaaaa ttggcctgag gtttactcaa
     1381 gacctgctcc gcctttaatt cctccaaacg cagaattact tgaatttcta aaaagctggc
     1441 gtgatgaaaa agcatcatca ctgagtattg acccctcgat gctcgccaat cgaattcaac
     1501 tcattgcttt agccactcca ggaacaacct cttgggaaga gcgatttgat aatgctcatt
     1561 ttatggtttg gcaaaaaaat atttggattg atattcttca acaaaacctg aattaaacta
     1621 ggagtcacta tgttcagaaa tatcggccta ccagaaatac tgcttttagt gctattattc
     1681 attgtggttt tcggttcgaa gcgaatccca gatattgcag aaagtatagg caaggcttta
     1741 aaaaaattca gaaaagcttc tcaagaaaca gaaaacgatt ccgattctac agaatcggaa
     1801 aataaagatg acgagtctaa atgatgaaat gccgatccta aagcattttc aggagcttcg
     1861 ttctaggctc ttaatttgcc ttgttttttt tattatctgc tttctaatct ccttttattt
     1921 ttctcatcta atctggtttt tattatctct tccaattaaa gaaatttctc gtttagagct
     1981 cattaattta gcccccgcag aagcacttga agtggatttt aaaatgtctt ctattgccgc
     2041 ctttatttta gcagccccca tttgctttta tcaattttac gctttttgtg cagaagccct
     2101 ctaccctaca gaaaaaagaa tcattattac tctcaccctt acttctaccc tctgtttttt
     2161 aattggcgtc tcctttgcct tttttacagt tcttcctctt ctatttcatt tcctagctga
     2221 atacaacgct gaagcaaagg ctctctggtc tcaaaatgcc tacatctcat ttttaattcg
     2281 tcttgaactc gcttttggaa tcatttttca gatgcctgtc attgcggcct ttttatctta
     2341 ctttaagatc attaaatcca atttttttat tcaacatttc cgaatcgcca tttttgtttt
     2401 agccttcatc tccgctctaa tcacccctcc tgatttactc tccatgattt ttatgatggg
     2461 accaatgcta cttctctatt taattacgat tatcgtaaat cgtatatttg gaaagggggc
     2521 ctaatggatt taggaattgg cttttcagag tttttgctac ttgcaattct tgcattacta
     2581 ttcttaaaac cagatcaaat ggtttataca ttaaagaaat ggaaaacatt aaaagggaaa
     2641 tattttaaat taaaatatga catcgaagat cacttcagcg cagccctcca aaaaggaact
     2701 cactccatga aaacggaaca atctcgatgg atcattaatg aaacgctcaa gttagaagct
     2761 tttcaaaaag caaatatcat tgccgccttt tttcctttgc caaacgaacc acaaatcgag
     2821 cctttattaa aagaaattat ggcaatgaaa acacttctat tgcctcgcgt ttcaagcgaa
     2881 accgagatgg attttgtaat gatttctgat ttagaaaaag acctagtcaa aggggctttt
     2941 ggcattttag aacccgcctc aaatctctct ccttggaata aatccattga tttattcatt
     3001 gtccctggcg ttcgtttttc aagagatggc ggtcgaatag gacacgggaa aggctattac
     3061 gatcggtttt tatcaaaata ccctcacgcc tataaaattg gcgtctgttt ttcctcacaa
     3121 atagaaaata aacctctttc attacagcca catgacgtca agatgaacta tattgtggct
     3181 ccggataatt caacaaagga tctctttata tgaacttcaa agaaaaccct tcttcttttg
     3241 gacgatcagc tcattttaga aatacagccc ctagagattc gattatgcgt cgccctagag
     3301 cagaagatgt ttctatcgcg atgagtgatg cagaaacagc tcctcaagtt gctgttggtg
     3361 gcatgaaaga agtggaagaa ttaattacta aagagcctta taaagtacat cgcgttttat
     3421 ttttacatca atctaaaaac ccacgtttat atactttgca aaaaatggct cgaaagaacc
     3481 acattcatgt acagcaagta gaagcacgta ttttagattc ttatgctcgt cctaatcaag
     3541 ggattgtagc cttatgtaat gaaaagtcct tactcgcttg ggaagatatc gctgaagaat
     3601 tcattcaagc aaaagaaaaa gacgtccaaa aaattattgc tgtaggcacc aatattgaag
     3661 accctagaaa cttaggagct tgcattcgtt catcgctcgc tctcggtgta gatttgcttt
     3721 taattccagc aaaaggcatg tgtggaatta ctcctactgt cgcccgtaca tctgcaggcg
     3781 ccctagaaaa gcttcgcatc tgtagaccta ataatttaga gggagtgatt gcacagttaa
     3841 aagaagcggg gtatcaaatc gttggtcttg atgccgctac agacattaat cttgctggat
     3901 ttgattttga aaagcaaatt gtaattgctg ttggaggaga agacc
//
LOCUS       DAOOBB010000177         3919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_25899_length_3919_cov_4.435559, whole genome shotgun sequence.
ACCESSION   DAOOBB010000177 DAOOBB010000000
VERSION     DAOOBB010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25899_length_3919_cov_4.435559"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1715)
                     /locus_tag="PLT31_07780"
     CDS             complement(<1..1715)
                     /locus_tag="PLT31_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95067.1"
                     /translation="MKFALYYITFLLFATSAFAGGVFEDKETKFVYRDSILDFSSEAC
                     PEGGIRLTPENSFYVDDSDLPYRVYRVALPDSIPPSVAIRDTKTKTMTSIWCQKDSIP
                     SLKTIVSKPFLRDGLWMIDISVPLLKSKASFYTVREQFEISVEFRSKKAKGYFPGKRA
                     LAKVLNSKSASKWGVSRNHNALRRAAVSELSNVEWIARFSIGNQEVASLSEDGLYAVP
                     FTLIRSALSAAGLEDKMSGVPVSKWRLYGASADTLQEEITSTSDILPAQLFQIPIEIR
                     DHNGRDLSPNGIFDDGDTLVFVGYGTSLWKKDSLFPKAPYYFSVSPYSFYQYFQLGWS
                     DQGSPKRLENLSTSTYSNPKDIAWKRYVRAEKEALLRDTYFGKELFWETSTGKEWFWF
                     WHSFKDTLSLSSSDLYFPSTSTLKGIKEGGQGVVSFSFLPHRSTAIYKVGDLSQSTNS
                     TYSALPYKDRMSKINFTASVNGASLIEHDTLMNGGNFVFSVSNLKANGNEYTVTMLPN
                     TGLFDRFDGYSIAYDWNTQVDSAEWFLPGMVSGVIRIPVSDESLRVLKFKNGEPLGLL
                     RVQNG"
     gene            complement(1752..2582)
                     /locus_tag="PLT31_07785"
     CDS             complement(1752..2582)
                     /locus_tag="PLT31_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95068.1"
                     /translation="MKKNALFLILLLGLVQWTSAQSLVTEWKDLSKFSSTSLFSKLDS
                     IGKNGNWMQYDSAGVLDAFMLKFEKKQLASWVLKEPSGSYLLAVLSKASKGERLSVYR
                     FKEINTTPEALKVHSVLDPNRLFLDYKQTSETEFEHLDNPNLKVSVLSDRIRFTYKNE
                     DPNTIFFPTDFKTQNEVEKRALVDQYLAFFEYEYSLMLRSFIQSTRGIFNWQPWHWYL
                     KPWTTGMKISRHELEAILERGVAPSSFRLFYTKTKNGEIVQFSTNGNGYYLMEILLPR
                     "
     gene            complement(2579..3364)
                     /locus_tag="PLT31_07790"
     CDS             complement(2579..3364)
                     /locus_tag="PLT31_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HPW95069.1"
                     /translation="MVNSQFYSSKRSILILVMVLFAISPLMAAPWLGFSFKKEPYQNQ
                     MALVVEGVHPSSGALAAGLSNGDKVISVNEKQITSVGVVQDVLKKKKVGESISIRFIR
                     DGKEQTAKIKLTDRPDDISNWTGSAIGSKMVDFGQNFYANGEKRQAKPKVILLDFWAT
                     WCMPCRQTLPILERLYNKYAKEGLEVVGISKESLSVLKSFYQKNASVYPLYRDADFKF
                     SNHYRITSVPTLMLLDQNGYIQKVWAGVPNEAQLDQIIREKLR"
     gene            complement(3336..3827)
                     /locus_tag="PLT31_07795"
     CDS             complement(3336..3827)
                     /locus_tag="PLT31_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95070.1"
                     /translation="MASGFFLDEENLVVPKEETSKWSYGFGTGFSFIFRQFDLSLYGT
                     LQYRISENFAVAGIVASDILESRYDFGARGLWYFYPEDFLMAGVSGVLLVQESGTVFP
                     PRVSFGYGKDFLPWERAHFFLRTTISISYVIGDYIESSPTELGITEINQVVLHGEFSI
                     LFF"
BASE COUNT         1356 a          785 c          664 g         1114 t
ORIGIN      
        1 tagccattct gaacacgcaa aagaccaaga ggttcaccgt ttttaaattt tagaactcga
       61 agagattcat cagaaactgg aatacgaatc acaccactta ccatcccagg taaaaaccat
      121 tcagcagaat ccacttgagt attccaatca taagcaatac tgtaaccatc aaaacgatca
      181 aataggccag tatttggtaa catggtcaca gtatactcat ttccattcgc ttttagattg
      241 gatacactaa aaacaaaatt acccccattc attaatgtat catgttctat taatgacgcg
      301 ccatttacgg aagctgtaaa attgatttta ctcattctat ctttataggg taaagcagaa
      361 taagttgaat tagttgattg agaaagatca cctactttat aaatcgctgt cgaacgatga
      421 ggtagaaaag aaaatgagac cactccctga cccccttctt ttatgccttt taaagtagaa
      481 gtagaaggga aatataaatc agaagaagaa agactcaaag tatctttaaa tgaatgccaa
      541 aaccaaaacc actcttttcc tgtggatgtt tcccaaaaaa gttcttttcc aaaatatgta
      601 tctcttaaaa gagcttcttt ttctgctcga acataacgtt tccaggcgat gtctttagga
      661 ttcgaataag tagaagtgct taaattttct aaacgcttgg gagagccttg atcagaccaa
      721 cccaattgaa aatattggta aaaactatag ggtgaaactg aaaaataata aggggcttta
      781 ggaaataaac tgtctttttt ccataaagac gtaccatacc ctacaaaaac aagagtatcg
      841 ccatcatcaa aaatcccatt aggcgaaagg tctctaccat tatgatctcg aatttctata
      901 ggaatctgaa ataattgggc tggtaagata tcagaagtag aggtgatttc ttcttgaaga
      961 gtatctgcag atgctccata aaggcgccat tttgaaacag ggacgccact cattttatct
     1021 tctaaaccag ccgcactcaa agcacttcga atcaaagtaa aaggaacggc gtacaagcca
     1081 tcttcactta aacttgcgac ctcttgatta ccaatagaaa atcgagcaat ccattctaca
     1141 ttggaaagct cagaaacggc agctcgacga agggcattat gattccgaga cacaccccat
     1201 ttcgaagcgg atttagaatt caatacttta gctaaagctc tttttccagg gaaatatcct
     1261 ttggcttttt tagaacgaaa ttcgactgaa atttcaaact gctcacgaac ggtataaaaa
     1321 gaagctttac tttttaacag aggcacagaa atatcaatca tccataaacc atctcgaagg
     1381 aaaggtttcg agacaattgt ttttaatgaa ggaatagaat ctttttgaca ccaaatcgat
     1441 gtcattgttt ttgttttagt atcacgaata gcaaccgatg gaggaataga atcaggcaac
     1501 gccactcgat aaacacgata cggtaaatct gaatcatcta cataaaaact attttctgga
     1561 gttaagcgaa tgcctccctc tggacaagct tcagaagaaa aatccaaaat ggaatctctg
     1621 taaacaaatt tggtttcttt atcttcaaaa acaccaccag caaaagcaga tgtagcaaat
     1681 agcagaaaag taatgtaata taatgcaaat ttcataattt tatttagtga ttcttctaca
     1741 agataaaaaa atcaacgcgg taaaagaatc tccattaagt agtatccatt gccatttgtg
     1801 ctaaattgaa cgatttcgcc attttttgtt tttgtgtaaa ataaacgaaa agaagaagga
     1861 gccactccac gctctaaaat ggcctctaat tcgtgacgag agatcttcat tcctgtcgtc
     1921 caaggcttta agtaccaatg ccatggctgc caattaaaaa tacctcgagt cgattgtata
     1981 aaggaacgaa gcattaaaga atattcatat tcaaaaaaag ccaagtattg atccactaag
     2041 gctctctttt ctacttcatt ttgagtttta aaatctgtag ggaaaaatat agtatttggg
     2101 tcttcatttt tataagtaaa tcgaattcgg tcggaaagta ctgatacctt taaattggga
     2161 ttatctaaat gttcaaactc cgtttcactt gtctgcttgt agtctaagaa taaacgattg
     2221 ggatctaaaa cagaatgaac ttttaatgcc tctggagttg tattgatttc cttaaaacga
     2281 taaaccgaaa gacgttctcc tttggaggcc ttcgataaaa cagccaaaag ataagaccca
     2341 gaaggttctt ttaaaaccca agaagcgagt tgtttctttt cgaactttaa cataaacgca
     2401 tctaagactc ctgcggaatc atattgcatc cagtttccat tttttccaat ggaatctaat
     2461 tttgaaaata aagaagttga agaaaactta gaaaggtctt tccattcagt aacgagagac
     2521 tgagccgaag tccattgcac aaggcctaat aacaatatca aaaataacgc gttttttttc
     2581 aacggagttt ctcccgtata atttgatcta gctgcgcttc atttggaacg cccgcccaga
     2641 ctttttgtat atacccattc tgatctaaaa gcattaacgt cgggacggat gtaatacgat
     2701 agtgatttga aaatttaaaa tcagcatctc ggtataatgg ataaacagag gcattctttt
     2761 gataaaatga ttttaataca ctcaacgatt ctttagaaat gccaaccact tctaagccct
     2821 ctttagcata cttgttataa agcctttcaa gaataggtaa ggtctgccta caaggcatac
     2881 accaggtggc ccaaaaatca agcaagatga cttttggttt cgcttgacgt ttttcgccat
     2941 tggcataaaa attttgaccg aagtccacca ttttacttcc aattgcagaa ccagtccaat
     3001 tagaaatatc atctgggcga tccgttaact ttattttcgc cgtttgttct ttaccgtctc
     3061 ggataaaacg aatagaaatg gattcaccca ccttcttttt ctttaaaacg tcttgaacga
     3121 ctccgacaga tgtaatctgt ttttcgttaa ccgaaatgac cttatctcca ttagaaaggc
     3181 cagcggcaag cgccccagaa gaaggatgca ctccttctac cactaaggcc atttgatttt
     3241 gatacggttc tttcttaaaa gaaaaaccca accacggtgc cgccatcaaa ggagagatcg
     3301 cgaacaaaac catcactaaa attaaaatgc ttcttttaga agaatagaat tgagaattca
     3361 ccatgaagta ccacttgatt aatttctgta atgcctaact ccgttggaga actctctata
     3421 tagtctccaa ttacataaga aatactaatt gttgttctta aaaaaaagtg tgctcgctcc
     3481 caaggcaaaa aatctttacc ataaccaaaa gaaactctgg gaggaaacac cgttccagac
     3541 tcttgtacta ataaaacgcc agagacacct gccattaaaa agtcttcagg ataaaaatac
     3601 cataagcctc tagccccaaa atcatagcgt gattctaaaa tatcagacgc gacgattcca
     3661 gcgacagcga aattctcgct aatgcggtat tgtaaagtgc cgtaaagaga gagatcaaac
     3721 tgacggaaaa taaaagaaaa gcccgtgcca aagccataac tccattttga tgtttcttct
     3781 ttcggtacaa ctaggttttc ttcatctaaa aaaaagccag acgccataga catcgaacag
     3841 aataagagaa ttatcggtaa gagcctattc accataaaaa tcagaaaggt ttttataacc
     3901 gttagcgatt aaagcctct
//
LOCUS       DAOOBB010000178         3909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_25996_length_3909_cov_4.366113, whole genome shotgun sequence.
ACCESSION   DAOOBB010000178 DAOOBB010000000
VERSION     DAOOBB010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25996_length_3909_cov_4.366113"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..2374
                     /gene="feoB"
                     /locus_tag="PLT31_07800"
     CDS             122..2374
                     /gene="feoB"
                     /locus_tag="PLT31_07800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HPW95071.1"
                     /translation="MIDKENFTIAIAGNPNCGKTALFNALTGSNQEVGNWPGVTVEKK
                     EGFFQLDNHKIRVVDLPGIYALSANSEDERVALDFLLSREADLIINILDATNLERNLF
                     LTTQLMELDIPMVIAVNMMDIAVRRGIQVYLKKMTELLGITAVGLSAISEKSCEGFVN
                     DMHDILHGVRPTPVPITHSASIENLVKELSPKFDSLSKKLNVSSRWVAIQYLEHSGRI
                     IDLVEEEGISISRELIEESLGEEPEFAIADSRYTLIRQVAVSVVRDRTKKETNTDKID
                     RVFLHRIFGLPLFVLAMYLVFWIAVTFGGAFIDFFDIFFGAFFVDGFASVLQSIGVSD
                     VWIAFLADGLGTGIQTVATFIPVIFFMFLCLAFLEDSGYMARAAFVADRFMRILGLPG
                     KAFVPMLVGFGCSVSALMATRTMENKRERFLTLFLVPFMSCSARLPVYALFGAAFFGA
                     KAGMMIFAIYFTGILVAIFYGLLLRKTLFQGQETSFIMELPPYHFPRFGKLCRLAWYR
                     LREFLFRAGKVVLLMVTFLGFLGSIGTDGSFGNQDSKQSVLSVVGREITPVFEPFGVE
                     KDNWPASVALFTGLFAKEAIVGTLNSLYSISDNKNSESEETFDLGESIKEAFISIPVN
                     MKGVFCDFLSPFSLGGAVDESASEENRGVINQMKKHFTLGTAQVFSYLLFILLYVPCL
                     AALGTAYRELGRFYATLMIVFQTILGWCLSVLFFQIMTGHQLFWIIVALILLLLESLS
                     LFWIGKKNKI"
     gene            2554..>3909
                     /locus_tag="PLT31_07805"
     CDS             2554..>3909
                     /locus_tag="PLT31_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HPW95072.1"
                     /translation="MNKNIKEAALAYHASGKPGKIEVVPTKPHSTQTDLGLAYTPGVA
                     EPCLEIERDESLAYDYTGKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKALLFKIYA
                     GIDVFDIEINEKDPKKFIEIVKGIAPTFGGINLEDIKAPECFEIEQTLVKALDIPVMH
                     DDQHGTAIISAAGLLNAIEIAGKNIAEVKMVVNGAGAAASACTRLYLALGLKKENLVM
                     VDSKGVINPNRSDLSPNKKEFMTQRTDVNTLKEALVGADIFLGLSKGNILTREMVRSM
                     ADVPIVFALANPTPEISYEEALASRGDLIFATGRSDYPNQINNVIGFPYIFRGALDVR
                     ASTINEEMKLAAVRAIAKLAHEPVPDVVNIAYNAKRFTFGKDYLIPKPLDPRLLTEVS
                     IAVAKAAIDSGVARKPITDWDAYYDRLRDMMGYDNKMMRGFTDTARSNPKRVVFAEGM
                     HI"
BASE COUNT         1118 a          636 c          843 g         1312 t
ORIGIN      
        1 gagttgtttt agaattactt caaatagcac ctcttggaga tcctctaaaa gtaaaagttt
       61 tatcctatcg tttatcgcta cgaaaaaaag aagcagaaat cttaaagtta agaagagtct
      121 tatgatagac aaagaaaatt ttacaatcgc cattgctggg aatccaaact gtggaaaaac
      181 agctcttttt aatgctttaa caggctccaa tcaagaagtg ggcaattggc ctggagtaac
      241 tgttgaaaag aaagaaggct tttttcagtt agacaatcat aaaattcgcg tggtcgattt
      301 gcctggcatt tacgctcttt cggcaaatag tgaagatgaa cgagtcgctc tagatttttt
      361 attatctcgt gaagcggatt tgatcatcaa tatcttggat gcgactaatt tagaacgcaa
      421 tttgttttta actacccaat tgatggaatt agatattcca atggtcattg cggtaaatat
      481 gatggatatt gctgttcgcc gaggaataca agtctactta aaaaaaatga cggagcttct
      541 tggaattacg gcggtcggtt tatcggcgat atcagaaaaa agctgtgagg gatttgtaaa
      601 tgacatgcat gatattcttc atggtgttag gccgactcct gttcctatta ctcacagtgc
      661 ttcaatagaa aacttagtaa aggaactttc tccaaagttt gattcgcttt ctaaaaagct
      721 aaacgtatct tctcgttggg tcgctattca atatttagaa catagtggtc ggataattga
      781 tttagtcgaa gaagaaggga tttctatttc tagagaatta atagaagaat cgcttggaga
      841 agaaccagaa tttgccattg cagattctag atatacattg attcgccagg tagctgtatc
      901 agttgtacga gatagaacaa aaaaagaaac caataccgat aaaatagatc gagtcttttt
      961 acatcgaatc tttggtttac ctttgtttgt gttagcgatg tatcttgtgt tttggatcgc
     1021 agtcactttt ggtggcgctt ttattgactt ttttgatatc tttttcggcg ctttttttgt
     1081 ggatggtttt gcctctgtat tacagtctat tggggtttca gacgtttgga ttgcattttt
     1141 agccgatggt cttggtactg gtattcaaac ggtggctacc tttatccctg ttattttctt
     1201 tatgtttttg tgcctagcat ttttagaaga ttcagggtat atggcaagag cggcttttgt
     1261 ggcggatcgt tttatgcgta tcttagggct tcctggaaaa gcctttgtgc cgatgcttgt
     1321 gggctttggt tgttctgtgt ctgctttgat ggccactcga acaatggaaa ataaaaggga
     1381 acgattttta acgctatttc ttgttccatt tatgagttgt agtgcaaggc ttccagtata
     1441 tgcacttttt ggtgccgctt tctttggcgc caaagcgggg atgatgatct ttgctattta
     1501 cttcactggt attttggtcg ccatttttta tggcttactt ctaagaaaaa cgttgtttca
     1561 gggtcaagaa acttcattta tcatggagct ccctccttat catttccctc gttttggaaa
     1621 gctttgccgt ctcgcttggt atcgattaag agagttttta tttcgagcag gtaaagtggt
     1681 attattgatg gttactttcc ttggcttttt gggctctatt gggacagatg gctcctttgg
     1741 aaatcaagat agcaagcaat cagtgctctc tgtagtgggt cgtgaaatta ctcctgtctt
     1801 tgaaccgttt ggtgtggaaa aagataactg gcctgcttcg gtggctttgt ttacaggact
     1861 ttttgcaaaa gaagccattg ttggcacttt gaattcttta tacagtattt ctgataacaa
     1921 gaatagcgag tccgaagaaa catttgattt aggggaatcg ataaaagaag cttttatatc
     1981 aattccagtc aatatgaaag gtgttttttg tgattttcta tcgccatttt ctttaggcgg
     2041 agccgttgat gaaagcgcct ctgaagaaaa tagaggtgtg attaatcaaa tgaaaaagca
     2101 ctttactctt ggaacggctc aagtgttctc gtatttatta tttattcttt tgtatgtgcc
     2161 ttgccttgct gcactaggga ctgcatatag agaattgggc cgtttttatg cgacattaat
     2221 gattgtattt caaacaattc tcggttggtg cctttcggtc ctcttctttc aaattatgac
     2281 agggcatcaa ttattctgga taattgtagc gttgatttta cttcttttag aatcactttc
     2341 tttattctgg ataggcaaaa aaaataaaat ctaaactttt agacctctct gcttttaagt
     2401 aagtaataaa tcagctttaa aatcactaga tagagtcaat agatgctatc ttttacaagc
     2461 gaatcctcta atcattatcc gataatagtg tttcggtctg tgcggtaaag tgattattaa
     2521 gaggcttcaa acaaggtgaa agaaggattg aaaatgaata aaaatattaa agaagccgct
     2581 ttagcttatc atgcgagtgg aaagccagga aaaatagaag tcgttcctac aaaaccacat
     2641 tccactcaaa ctgatttagg attagcttat acaccaggcg ttgctgaacc ttgccttgaa
     2701 attgaacgag atgaatcact cgcttatgat tatactggaa aaggaaattt agttgctgtt
     2761 atttctaatg gaacagctgt tttaggatta ggcgatatag gcgctttagc tggtaaacca
     2821 gttatggagg gcaaagctct tcttttcaaa atttatgcag ggatagacgt atttgatatt
     2881 gaaatcaatg aaaaagatcc taaaaaattt attgaaatag tcaaaggcat tgctcctaca
     2941 tttggtggca ttaatttaga agacattaaa gctccagagt gttttgaaat agaacaaact
     3001 ttagttaaag cattagatat tcctgtcatg catgatgatc agcatggaac agctattatt
     3061 tctgctgctg gtcttttaaa tgcaattgaa attgcaggga aaaatattgc agaagtgaaa
     3121 atggtggtga atggtgctgg tgctgctgca agtgcttgta caagacttta tcttgcgctt
     3181 ggtttgaaaa aagaaaacct agttatggtg gatagcaagg gtgtaattaa tccgaatcgt
     3241 agtgatttga gccctaataa aaaagaattc atgacacaaa gaacagatgt caatacttta
     3301 aaagaagcgt tagttggcgc tgatatcttt ttaggccttt ctaagggtaa tattttaact
     3361 cgagaaatgg tgcgtagcat ggcagatgtt cctattgtat ttgcactagc taatcctaca
     3421 ccagaaatat cttatgaaga agcccttgct tctcgtggcg atttgatttt tgcaacgggc
     3481 cgttctgatt atcctaacca aattaacaac gtaattggat tcccatatat tttcagaggt
     3541 gctcttgatg tgcgcgcttc tacaattaat gaagaaatga aattagcggc tgtacgtgcg
     3601 attgctaaat tggctcatga acctgtacct gatgtagtga atattgcgta taatgccaag
     3661 agatttacct ttggtaagga ttacttaatc cctaagcctt tagatcctcg cttgctaaca
     3721 gaagtttcca ttgcggttgc aaaagctgct attgatagcg gggtcgctag aaagcctatt
     3781 acagattggg acgcttatta tgaccgtctt cgtgatatga tgggttatga taataagatg
     3841 atgcgtggct ttacggatac ggctcgtagt aatccaaaac gagttgtatt tgctgaagga
     3901 atgcatatc
//
LOCUS       DAOOBB010000179         3854 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_26491_length_3854_cov_4.450382, whole genome shotgun sequence.
ACCESSION   DAOOBB010000179 DAOOBB010000000
VERSION     DAOOBB010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26491_length_3854_cov_4.450382"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PLT31_07810"
     CDS             complement(<1..241)
                     /locus_tag="PLT31_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HPW95073.1"
                     /translation="MIKRFFTLLISSMALGSLMGCEKEKPPVTEVRSYSGSVEVLNSC
                     GKTGAASQMTIFLRNKGFDVVQYRNDLLQNYEETII"
     gene            complement(301..651)
                     /locus_tag="PLT31_07815"
     CDS             complement(301..651)
                     /locus_tag="PLT31_07815"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HPW95074.1"
                     /translation="MQIELLKTKIHRATVTDANLNYEGSITIDKTLMDAAGLLPYEKV
                     GILDIHNGARFDTYVIEGEPDSGTICLNGAAARLVQPGDLIIIVAYVSLTPEEAKNWH
                     PVVVRVDSKNKIIV"
     gene            complement(661..1377)
                     /locus_tag="PLT31_07820"
     CDS             complement(661..1377)
                     /locus_tag="PLT31_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95075.1"
                     /translation="MAHWLKAIPAILILSAISFAQYDYNYDSEGETEIPATGAATATG
                     DEWDGFRIEEMGLTQWEFQQAKEGGISRNKLTQLVELGIRPTEYLQKPWNRLGVSEEA
                     WLEQRAQGLEDSDIDRSYRNKNDQQNLAYLSLLIPSLYQWKTGANSKAVWMDVLWASG
                     VGAAIFLGVSEPDQKTWIYALIPILAAHVWSFADAFFGTQWENNPDANRFSFGVLPTF
                     NEGVAGGLDKGVAGLLQMRF"
     gene            complement(1406..1489)
                     /locus_tag="PLT31_07825"
     tRNA            complement(1406..1489)
                     /locus_tag="PLT31_07825"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1453..1455),aa:Leu,seq:caa)
     gene            complement(1567..2400)
                     /locus_tag="PLT31_07830"
     CDS             complement(1567..2400)
                     /locus_tag="PLT31_07830"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPW95076.1"
                     /translation="MFRFILLFSIALSYAQTITLADAQKAYLSANWAEAAKLFSTVCP
                     QLEKGKQPECVLWTILSLSQTGRMEDFSTAIKKLDSLIQTSSPQDPIYTDLYMTKAQF
                     ELYLNKYDLSVISLRHAIETSRVSQHPVLLKVCSAIEKAYRKPEVQALCLKLQPKNDT
                     LTSISSSFIASSSSTITARSSSTILPSSSSLTQQNTISNKKEWVLQLGAFSQKENAAL
                     LVDNLKKRKISTQIKEKIISEKTLYLVQTLPFTSESEAIEYGKKFFTPLNMEFRALKW
                     E"
     gene            complement(2402..3703)
                     /gene="miaB"
                     /locus_tag="PLT31_07835"
     CDS             complement(2402..3703)
                     /gene="miaB"
                     /locus_tag="PLT31_07835"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HPW95077.1"
                     /translation="MKYHIATFGCQMNEYDSGLLANLLESVGCQVTSKKEEADFIFFN
                     TCSIREKAEDTAIARISQIKSLKYKNPNLKIIIVGCMAKNKGKALFKRLPFIDYVIGP
                     DHYKEIPEIILKQKSYQRVYTEFDETENYQGAYAKLQTPYSTSIGIQRGCNKRCSYCI
                     VPYVRGKEKNRDPLDILAEVQSAADRGVSEIMLLGQTVNAYHKDDFSFATLLDKISQI
                     NGIERIRFTSPHPKHYNDELIDVLLSNSKICNHVHLPLQSGSDAILKKMRRQYNVENF
                     LSIVERFRNNNPLYAVTTDIISGFVGETDEDFEQTLEVVKKAQFDNAFMFIYSPRAGT
                     ESFQEKETLSEEEKQERHERLVVIQNEITMQRNKLMFNRNEKILVERPSTKDPDEWVD
                     KTENFKKVIFKPTKIVHPGDYVVCHINDIRGWTLRGEVVGE"
BASE COUNT         1186 a          713 c          723 g         1232 t
ORIGIN      
        1 cgataatggt ttcttcataa ttttgtaaaa ggtcatttcg atattgaacg acatcaaaac
       61 ctttattacg taaaaaaata gtcatttgag aggctgcacc tgttttaccg cagctattta
      121 atacttctac agaaccacta taagagcgaa cttccgtcac aggaggcttc tctttttcac
      181 aacccatcaa actaccaaga gccatcgagg aaattagaag tgtaaaaaaa cgtttaatca
      241 caagttaaaa ttagaattta tgaatgaaaa taattaaaga aagtgattaa aaagaagaaa
      301 ctaaacaatg attttatttt ttgaatctac gcgaactaca acaggatgcc aattttttgc
      361 ttcttctgga gtgagggata cgtaagcgac tataataatc aaatctcctg gttgaacaag
      421 ccttgctgcg gctccattca agcaaatggt tccagagtca ggttcacctt cgatcacgta
      481 agtatcaaaa cgtgctccat tatgaatatc taagattccg actttttcat aaggcaaaag
      541 ccctgctgca tccattaggg ttttatcaat agttatagaa ccttcgtaat ttagattcgc
      601 atcagtaaca gtagcgcgat gaatttttgt tttaagtaat tctatctgca tttttttaga
      661 ttaaaaccgc atttgtaaaa gacctgcgac gcctttatct aagccgcctg caacaccttc
      721 attaaatgta gggaggacac caaaactgaa acgatttgca tcaggattgt tttcccattg
      781 tgttccaaag aatgcgtcag caaaagacca aacgtgagca gcgagaatag gtattaacgc
      841 gtaaatccaa gttttctgat ctggttcact aacacctaaa aagatggctg ctccaacacc
      901 cgaagcccag aggacgtcca tccaaacggc ttttgaattg gctccagttt tccattgata
      961 aaggcttgga attagtaaag acaagtaggc taggttttgt tgatcgtttt tatttctgta
     1021 agaacgatca atatctgaat cttcaagacc ttgtgctctt tgttcgagcc atgcctcttc
     1081 acttacaccg agacgattcc aaggtttttg taaatattct gtaggacgaa tgccaagttc
     1141 gactagctgg gtgagtttat ttcttgaaat tccaccttct ttagcttgtt gaaattccca
     1201 ctgtgttaga cccatttctt caatacggaa accatcccat tcatcaccag tagctgttgc
     1261 agcaccagtc gcaggtattt ctgtctcgcc ctcagaatcg taattgtaat catactgcgc
     1321 aaaggagata gcactcaaaa ttaagattgc cggaatcgct ttcaaccaat gagccatctt
     1381 gacctcataa tttataaatc ataaatgccg ggggagggaa tcgaaccctc acagagttgc
     1441 ctctactgga ttttgagtcc agcgcgtcta ccaatttcac caccccggct agggggtaga
     1501 tcaaatatag cacagttttt gaatttttgc aggaaaaaga ctgattttga tgtaaagaaa
     1561 aagcaatcat tcccatttaa gagcccgaaa ttccatgttt aatggtgtaa aaaatttttt
     1621 tccatattcg atagcttctg attcggatgt gaatggaagg gtttggacta agtagagtgt
     1681 cttttctgaa atgatttttt ctttaatttg ggtagaaatt tttctttttt tcaaattatc
     1741 tacaagtaaa gccgcattct ctttttgact aaaggctcct aattgtaaaa cccattcctt
     1801 tttgttagaa atcgtatttt gctgtgtcaa agaagaggaa cttggtaaaa tggtagatga
     1861 agaacgggct gttatcgtgg aggaagaact tgcgataaag gatgaagata tagaagtcaa
     1921 tgtatcattc ttcggttgta gcttaaggca aagcgcttga acttcaggtt tacgataagc
     1981 tttttcaatt gcagaacaga cttttaataa aacgggatgt tgtgaaactc gactggtttc
     2041 aatcgcatgt cttagactaa tcacggataa atcatattta ttgagatata actcaaactg
     2101 agcctttgtc atataaagat cggtatatat tggatcttgc ggagaagagg tttgaattaa
     2161 actatccagt tttttgattg cggtagaaaa gtcttccatc cgtcctgttt ggctcaaaga
     2221 gaggatagtc cataatacac attcaggctg ttttcctttt tccagttgag gacaaacggt
     2281 gctgaataat ttcgctgctt ctgcccaatt tgctgataaa taggcctttt gtgcatctgc
     2341 aagagtaatc gtttgggcat aagataatgc gattgaaaaa agtaaaataa aacgaaacat
     2401 attattctcc tacgacctca ccgcgaagag tccaaccacg gatatcattg atatgacata
     2461 caacgtaatc tcctggatgt actattttcg tcggtttaaa tatgactttt ttaaaattct
     2521 cggttttatc tacccattcg tcagggtctt ttgtagaagg acgttctaca agaatttttt
     2581 catttctgtt aaacatcaat ttattgcgtt gcatcgtgat ttcattttga atcacgacaa
     2641 gcctctcatg gcgttcttgc ttttcttctt cagaaagagt ttctttttct tgaaaagatt
     2701 cggtccctgc tctaggacta taaataaaca taaatgcatt atcaaattgt gcttttttta
     2761 ccacttctag agtctgttca aagtcttcgt ccgtttctcc aacaaagcca ctgatgatat
     2821 cggtggtgac tgcatataag gggttattat tacggaatcg ttctacaatg gaaagaaaat
     2881 tctctacatt atattgacgt ctcatcttct ttaagatcgc atcactacca ctttgaagag
     2941 gtaagtgaac atgattgcaa atcttagaat tagaaagcag tacatcaatc aattcatcgt
     3001 tatagtgctt tggatgaggg cttgtaaaac gaatgcgttc aataccatta atttgggata
     3061 ttttgtctaa aagggtcgca aaagaaaagt catctttatg ataggcattt acggtttgcc
     3121 ctaacagcat gatttctgat acgcctcgat cggcagcact ttgaacttca gcgagaatat
     3181 cgagtggatc tcggtttttt tctttcccgc gaacgtaagg aacgatacaa taactacagc
     3241 gtttattaca ccctctttgg atacctatag acgtactata aggggtttgt aatttagcgt
     3301 aggctccctg ataattttct gtttcatcga attcggtata gacgcgttga taagactttt
     3361 gttttaaaat aatttcagga atttctttgt agtgatctgg cccaatgaca taatcaataa
     3421 aaggaagcct cttaaacagg gctttgcctt tgtttttagc catacaaccg acaatgataa
     3481 ttttcaaatt cgggttttta tattttaagg atttgatctg agaaattctt gcaatggctg
     3541 tgtcttcagc cttttcacga atagaacaag tattgaaaaa aataaaatca gcttcttctt
     3601 ttttggatgt aacttgacaa ccaacacttt caagtaggtt agctaataac ccagagtcgt
     3661 attcgttcat ttggcaacca aatgtagcga tatgatattt cataagtgca cctctaaaac
     3721 aatactagga acgaaaagtg gagagtactg gagatattca acatctttga gagataacga
     3781 agcgtgatgt attatagaat agatcatgaa aatgaattat tagaggtgac cataagacaa
     3841 aaactagcaa attc
//
LOCUS       DAOOBB010000180         3831 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_26686_length_3831_cov_3.359375, whole genome shotgun sequence.
ACCESSION   DAOOBB010000180 DAOOBB010000000
VERSION     DAOOBB010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26686_length_3831_cov_3.359375"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="PLT31_07840"
     CDS             complement(<1..1028)
                     /locus_tag="PLT31_07840"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HPW95078.1"
                     /translation="MAFQYEKNIQYGQDKTQYVNIGKEGVSLADFDGEKVLKVERSAL
                     VKLTEAAFSEVSFRLRPAHTKQVASILKDPEASDNDKFVALTLLQNACVSNKGVLPFC
                     QDTGTAIAFVKKGQRIWTNCDDAEAISEGVYNAYTKLNLRYSQVAPLSMYEEKNTACN
                     LPAQVDLLAEPGDHFDFLFVAKGGGSANKTYYWPMTKALLNPKALEKFITEKIKSLGT
                     AACPPYHLALVIGGFSAESNLKLVKLASCGYLDNLPTSGSENGRIFRDIEMEQKVLHI
                     CQKSGIGAQFGGKYFVHDVRVIRCPRHAASCPVSIGVSCSADRNIRAKIDADGLWIEK
                     MEHNPEQYL"
     gene            1211..1882
                     /locus_tag="PLT31_07845"
     CDS             1211..1882
                     /locus_tag="PLT31_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95079.1"
                     /translation="MNFKKITFLALLVSTLAFAADANTTKTAATPAPVKKEAVAPAKK
                     EAAAPAKKEAAAPAKTEAVAPAKTEVAAPAKTEAAAPAATEAAAPAKTEAAAPVATTD
                     SAAAAPAATTDSAAVAPAATTDSAAVAPAAIDTTKEVLETTVKEEKAPVDSAALAKAE
                     ADKIAAEKAAKAKADSVAAAEKALEEPMAPTAQKAIMENPWEFLIVSAAFIASVLVII
                     FTGKE"
     gene            2401..>3831
                     /locus_tag="PLT31_07850"
     CDS             2401..>3831
                     /locus_tag="PLT31_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HPW95080.1"
                     /translation="MPIGYFLVLCFAVFSFAQQNLSTPYDLIRPVYPLTWDSTVFDNY
                     DTTVTTKKGMVPKNRTPASFAPTALIPDTLNQAYLDAINLKISPIRINQAGYLEKDPE
                     RKFYYVGTATSFEVVDSLGNSFTPKYTGTFQSSGTTTASDWTIIAGTNAATNDQLRYQ
                     VSTVGPSGTIQIGNLPLDLPTNQRLRVKVGNDISATFIISNEIYSMVRSATLKFYGIN
                     RSGDSESWFHPASHTKDGGGPVVTEAADERGPFNPALAGTLQGGYYDCGDHLKESQTQ
                     MYAFMVAAVLAATNPLADDDKYEYNHGNTAIDGIPDMLREAKHGADFVLRSYVRAKGV
                     IDDMALSVGNFGSDHGWWGRPEHQDNLPTGNTATATDRGGPASRTVRLGEIGANIGGE
                     TAAGLAILAKDYAQFDKPFADSCLMVAKKMYDFAKSLATGKTTYDGGKPFVNNKIAAG
                     WSSPAYNGNNEFFDDLALASIPLFYAT"
BASE COUNT         1176 a          820 c          693 g         1142 t
ORIGIN      
        1 aggtattgtt ctggattatg ttccattttt tcaatccaaa ggccatcagc atcaattttt
       61 gctcggatgt tgcggtccgc agagcaagaa acaccaatag aaacagggca actagccgca
      121 tgacgagggc aacgaatcac acgaacatcg tgtacaaagt atttaccccc aaattgagcg
      181 ccaattcctg atttctgaca aatatgaaga accttttgtt ccatttcaat atcacggaaa
      241 atgcgtccat tttcagaacc gctcgttggg agattatcta aataaccgca actagcaagc
      301 ttcactaatt ttaagttgga ttcggcacta aacccaccaa tcacaagtgc aagatgataa
      361 ggagggcaag ctgctgttcc tagagacttt atcttctctg taataaattt ttcaagtgct
      421 tttggattaa gaagagcttt tgtcataggc cagtagtagg tcttattagc agatccacca
      481 cccttagcca caaataagaa gtcaaaatga tcccctggtt cagcaagaag atctacttga
      541 gcaggtaaat tacaagccgt atttttttct tcatacatag atagtggagc gacttgacta
      601 taacgaaggt ttaatttggt ataagcatta taaacacctt ctgaaatggc ttccgcatca
      661 tcacaatttg tccaaatacg ctgccctttt ttaacaaaag caatggccgt tcctgtatct
      721 tggcaaaaag gtaaaacgcc tttattcgaa acacaagcat tttgaagcaa agtaagcgcc
      781 acaaatttat cattatcact ggcttcaggg tcttttaaaa tagatgccac ttgctttgta
      841 tgagcaggac gaagacggaa actgacttca gagaaagcag cctccgtcag cttcactaaa
      901 gcagaacgct ccacttttag aaccttttca ccatcaaaat cagctaaaga aacaccttct
      961 ttgccaatat ttacgtactg agtcttatct tgaccatatt gaatgttttt ttcgtattga
     1021 aatgccattt tagcctctta tcaaaagtaa caatggaaag ttaatctatt tttcgcctta
     1081 ttttttataa aaaaattgaa aaatccctat ttttggaatt aaattaaaaa aaatgatacg
     1141 ggtaagctag ataagaatta tcttttgaat cattgtttac ctaatcggtt cactataaga
     1201 cgaggactat atgaatttta aaaaaatcac ctttttggct ttacttgtat cgacattagc
     1261 ctttgccgca gatgcgaata caacaaaaac agcagccacc ccagctcctg ttaaaaaaga
     1321 agctgttgct cctgcaaaaa aagaagctgc tgctcctgca aaaaaagaag ctgctgctcc
     1381 tgctaaaact gaagccgttg ctcctgctaa aaccgaggtc gctgctcctg ctaaaaccga
     1441 agccgctgct cctgctgcaa ccgaagctgc tgctcctgct aagactgaag ctgctgctcc
     1501 tgttgcaacc actgactctg ctgccgctgc tcctgctgca accaccgact ctgctgccgt
     1561 tgcccctgct gcaaccactg actctgctgc cgttgctcct gccgctatcg acactacaaa
     1621 agaagtctta gaaacaacag ttaaagaaga aaaagctcct gtggattctg ctgcccttgc
     1681 aaaagcagaa gcagacaaaa ttgccgctga aaaagcagca aaagcaaaag cagatagtgt
     1741 tgcagctgct gaaaaagcat tagaagaacc aatggctcca actgctcaaa aagctattat
     1801 ggaaaaccca tgggagttct taatcgtttc cgcagcattt attgcctctg ttttagtgat
     1861 tatttttact ggtaaagagt aatatctatt ttactttact ttatagaacg agatcaaaca
     1921 tctcgttctt ttttttattc aaaaatgata aaaatagttc atgactggct gtccaaaaaa
     1981 actaacaatg aagaaaaaaa ataatgaaca aaagtccctt ctaaatcagt ttttcactga
     2041 tacaaaacat gattttatat tttgactaaa aaaattttat tctttataag cataataaaa
     2101 gaaggcttta tttttctctg aatcaaggaa tattttcata ataatacttt tttagatatc
     2161 ctttgaacca agttcttttt ctcaccattc tatcctcacg tttgttttct tttgtttttc
     2221 atcacgccat tttgaataac gagaaaaatc tccccaaaaa cccctatttt cacaaaaaaa
     2281 acgcattttt tgacattatt tttttattag gaatgttcat tctagcggaa taaatttatt
     2341 tttgtagttc gtgtgttttg ggatacaatt tgttttgctg agtaaaaaga aggaggcttt
     2401 gtgcctattg gatatttttt agtattgtgt ttcgctgtat tctcatttgc gcaacagaat
     2461 ctctcaactc catacgattt aattcgacct gtatatccac taacatggga tagtactgtt
     2521 ttcgataatt atgatacaac agtcacaact aaaaaaggca tggtgcctaa aaataggact
     2581 ccagcaagtt ttgctccaac agcactaatt ccagacacac taaatcaagc ctatttagat
     2641 gccattaatc taaaaatttc tcctatccga attaaccaag caggttatct cgaaaaagac
     2701 cctgaacgca aattctatta cgtgggtaca gcaacctcat ttgaagtagt tgattctctt
     2761 ggtaattctt ttactccaaa atacactgga acattccaat ctagtggtac tacaaccgct
     2821 tctgactgga ctattattgc tggcactaac gctgctacaa atgatcaatt acgctatcaa
     2881 gtttctactg taggcccctc tggaacaatt caaattggaa atcttccttt agacctcccc
     2941 accaatcaac gactacgtgt caaagtgggt aatgatattt cagccacatt tatcatcagt
     3001 aatgaaattt attctatggt gcgttctgcc acgcttaagt tctatggaat taatagaagt
     3061 ggtgattcag aatcttggtt tcatcctgca agccatacaa aagatggtgg cggtcccgtc
     3121 gtcacggaag ctgccgatga acgcggtcct tttaaccctg ctttagcggg gactttacaa
     3181 ggtggttact acgattgtgg agaccacctc aaagaatctc aaactcaaat gtacgccttt
     3241 atggtggcag cagtgttagc cgccaccaat ccattagcgg atgacgataa atacgagtat
     3301 aaccatggta atacagccat agatggtatt ccagatatgc tacgtgaagc gaaacacggt
     3361 gctgattttg tacttcgttc ctatgttcgc gccaaaggtg taatcgacga catggctctt
     3421 tctgtcggta attttggatc ggatcacggt tggtgggggc gtcctgaaca ccaagataat
     3481 ttacctactg gaaataccgc cacggcgact gaccgaggtg gtcctgcttc tagaacggtt
     3541 cgtctcggtg aaattggcgc taatattggt ggtgaaacag ctgcaggtct tgcgattctt
     3601 gcaaaagact atgctcagtt tgacaaaccc tttgcagaca gttgcttaat ggtagctaaa
     3661 aagatgtatg actttgcaaa aagccttgct acaggaaaaa ccacctacga tggcggaaaa
     3721 ccttttgtga ataacaaaat agccgctgga tggtcttccc ctgcttataa tggaaacaac
     3781 gaattttttg acgacttagc tttagcttct attccccttt tttatgccac a
//
LOCUS       DAOOBB010000181         3827 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_26714_length_3827_cov_4.203340, whole genome shotgun sequence.
ACCESSION   DAOOBB010000181 DAOOBB010000000
VERSION     DAOOBB010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26714_length_3827_cov_4.203340"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PLT31_07855"
     CDS             <1..215
                     /locus_tag="PLT31_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional anthranilate synthase component
                     II/anthranilate phosphoribosyltransferase"
                     /protein_id="HPW95081.1"
                     /translation="GKENVALAMDLIQGKGRPGILYSCALNAGATLYIGGKVKSIKEG
                     FDRALKALQDGSVMKKIEEVRKATHE"
     gene            220..1701
                     /locus_tag="PLT31_07860"
     CDS             220..1701
                     /locus_tag="PLT31_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol phosphate
                     synthase/phosphoribosylanthranilate isomerase"
                     /protein_id="HPW95082.1"
                     /translation="MMKTENILDKIVEQRKKDLERLGPSLGFSIPKTRTRPIVQFLNP
                     SGTILEIKRASPSKGDIAPQLDAVKTAKIYANAGTQAISVLTETHFFKGSLQDLMNVS
                     SEISDCSILRKDFLLKEEEIEISYLCGADAVLLIARILDTETLLRLARKAREFGMTPF
                     IEIRENDDLLKLEEAIQDGDVVAGVNSRDLATFHIDPLIPASFRNSLPCKAVFESGIK
                     TIEDATFAKNLGFEGILVGENVAKKPSFTPQLVKAFNDAKPNREGDFWKALALRREIH
                     REKDPHRPLVKICGITNIEDAVQAAELGADLLGFVFSDSPRQASGDLIQEVRKILNSM
                     ELYPLLVGVITNPHSSEGIQAITKAEYGILDAIQFHDCHIPSLLDTSYGHYGAVRVSH
                     SSHLDGVKKYLSLGEPRVLIDARVPNVLGGTGVSIPEHIVKETKEFTPLWLAGGISCE
                     NVKEIISNFEPELIDISSALELKPGKKSIEKMNLFFKELHHES"
     gene            1691..2980
                     /gene="trpB"
                     /locus_tag="PLT31_07865"
     CDS             1691..2980
                     /gene="trpB"
                     /locus_tag="PLT31_07865"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HPW95083.1"
                     /translation="MSHNGFFGPFGGKYVAEVLRRPLDELQVAFENAMHDSLFLEELR
                     IMQRDFIGRPTPLYFAENATKALGGAQIYIKMEGLAHTGAHKINNALGQALLAKKMGK
                     TRIIAETGAGQHGLATAAACAKLGLPCEVFMGEVDVRRQQPNVAAMEMYGAKVIPVKA
                     GTKTLKDAINEAMRDWAAHFDNTHYLIGSALGPAPFPDIVRYFQSVIGKEVEEQSQER
                     KLDIAALCACVGGGSNAIGFFSPYIEESSPRLIGAEAGGIGPGPHENAVRMSGSAAKD
                     GIVHGYKSRFLLDDDGQVAETRSISAGLDYPGIGPQLASLGQKGRIEFRAITDNQALE
                     ALQFFAKHEGILFAMESAHAGAVALQIAKEFPKEKAVIINMSGRGDKDIFITSPVFRP
                     LEWEQFLQSELDRLKNGTDIHYAESLPTNLKKELENK"
     gene            2982..3761
                     /gene="trpA"
                     /locus_tag="PLT31_07870"
     CDS             2982..3761
                     /gene="trpA"
                     /locus_tag="PLT31_07870"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HPW95084.1"
                     /translation="MTSQNQIRLMSHLVAGYPNLEISLEVADALVRGGSTFLEIQLAF
                     SDPSADGPAIQTACADALSKGFSTKKGLAFIAEVHKRHPNIPLFLMTYASLAYTPGIE
                     NFVKEAKEAGVSGVIIPDLPFDQDEGLTAACQKWGVENIPVAAPSMTQERLNKMLDAK
                     FTYIYAALRAGITGSVTTIDNQTLQFLDSFSKGSSKILGGFGIRSGSQAKQLADHVYA
                     IVAGSVFVNEITTHYDPKNEIESKIKIGAAVEAKAKEITLS"
BASE COUNT         1212 a          734 c          768 g         1113 t
ORIGIN      
        1 caggtaaaga aaatgtggct ttagccatgg atttaattca gggtaaaggg cgtccaggta
       61 ttttatattc atgtgcctta aatgctggag ccactcttta tattggtggt aaagttaagt
      121 cgatcaaaga aggtttcgat cgagctttga aagccctcca agatggttca gtgatgaaaa
      181 aaattgaaga agtacgaaaa gcaacacacg aataataaaa tgatgaaaac agaaaacata
      241 ttagataaaa ttgttgaaca acgaaaaaaa gatttagaac gcttaggtcc ctctttaggt
      301 ttttctattc ctaaaacaag aacacgtcct attgtacagt tcttaaatcc tagtggaacc
      361 attcttgaga tcaagagagc ttctccttct aaaggcgata tagcccctca attagatgcc
      421 gtaaaaacag ctaaaatcta tgcaaatgca gggactcaag cgatttcagt tctcactgaa
      481 actcattttt ttaagggttc cttacaagat cttatgaacg tatcttcaga gatatctgat
      541 tgttctattt tacgtaagga ttttctctta aaagaagaag aaattgaaat ttcttatctc
      601 tgtggagcgg atgccgtttt attaattgca cgcattctcg atactgaaac acttttacgt
      661 ctcgcaagaa aagcaagaga attcggcatg actcccttta ttgaaatacg agaaaatgac
      721 gatctcttaa aattagaaga agctattcaa gatggagatg tcgttgcagg agtgaattct
      781 agagacctag ccacatttca tatcgacccg ttaatccctg cttcttttag aaatagcctc
      841 ccttgtaaag ctgtttttga atcaggaatt aaaactatcg aagatgcgac ttttgctaaa
      901 aatctcggtt ttgaaggaat tttagtcgga gaaaatgtgg cgaaaaagcc ttctttcact
      961 ccccaactcg taaaagcttt taacgatgcc aaaccaaata gagaaggtga tttctggaaa
     1021 gctcttgcac tcaggcgtga aatccataga gaaaaggatc ctcaccgacc tcttgtaaaa
     1081 atatgtggaa ttacaaacat tgaagatgca gttcaagctg cagaactcgg tgccgattta
     1141 ctcggatttg tattttcaga tagtcctcga caagcctcag gagatctcat tcaagaagtc
     1201 agaaaaattc ttaattctat ggaactctac cctcttttag tgggagtgat taccaatcct
     1261 cattcttctg aagggattca agcgatcaca aaagcagaat acggtatttt agacgccatt
     1321 caattccatg attgtcacat accatctctt ctcgacactt cttatggcca ttatggcgcc
     1381 gttcgagtaa gtcatagttc tcatctggat ggtgttaaaa aatacttgtc tctaggagag
     1441 ccccgagttt taattgatgc gagagttcct aatgtcttag gtggtacagg agtttctatc
     1501 cccgaacaca tcgtgaaaga gactaaagaa ttcaccccac tttggcttgc tggtggtatt
     1561 tcttgtgaaa atgtcaagga aatcatttcc aatttcgaac ctgaattaat cgatatttct
     1621 agcgccttag aattaaagcc aggtaaaaaa tcgatcgaaa aaatgaattt attctttaag
     1681 gagttacatc atgagtcata atggattttt cggacccttt ggcggaaaat atgttgctga
     1741 agttctacga agacctttag atgaactaca agtggcattt gaaaatgcaa tgcatgattc
     1801 tttattttta gaagaattac gcattatgca acgagacttt atcggtagac caactcctct
     1861 ttattttgct gaaaatgcga caaaagcatt aggtggagct cagatttaca ttaaaatgga
     1921 aggcctagct catacaggag ctcataaaat aaataacgct cttgggcaag cacttttagc
     1981 gaaaaaaatg ggtaaaacaa gaatcatcgc agaaacaggt gctgggcaac atggtttagc
     2041 cacagccgct gcttgcgcaa agcttgggct accctgtgaa gttttcatgg gtgaagtaga
     2101 cgttagacgt caacaaccca atgtagctgc aatggaaatg tatggagcga aagttattcc
     2161 tgtaaaagca ggtacaaaaa cattgaaaga tgcaattaat gaagccatgc gagactgggc
     2221 cgctcatttt gataatacgc attatcttat tggttctgca ttaggccctg ccccatttcc
     2281 tgatatcgtc cgctactttc aatcagtcat tggtaaagaa gtagaagagc aatctcaaga
     2341 acgaaaatta gatatagctg cactatgtgc ctgtgtaggt ggcggttcta atgcgatcgg
     2401 tttcttttca ccttatatcg aagaatcatc tcctcgctta attggtgctg aagctggcgg
     2461 tattggccct ggacctcatg aaaatgcggt tcgtatgagt ggttcagcgg caaaagatgg
     2521 catcgttcat ggttataaaa gtcgcttttt attagatgac gatggtcaag tcgcagaaac
     2581 tagatctatt tctgctggct tagattaccc tggaataggg ccacaactcg cctctttagg
     2641 gcaaaaagga cgcatcgaat ttagagccat cacggataat caagccttag aagcattaca
     2701 attttttgca aaacatgaag gcattctctt tgctatggaa agtgctcacg ctggagctgt
     2761 cgctcttcaa attgctaaag aatttcctaa agaaaaagcc gttattatta atatgtctgg
     2821 acgtggagat aaagatattt ttatcacttc tcctgtattt agacctttag aatgggaaca
     2881 attcctacaa agtgaattag ataggctcaa aaatgggaca gatatccatt atgcagagtc
     2941 tcttccaaca aatcttaaaa aagaattgga gaacaaataa catgactagt caaaaccaaa
     3001 ttcgattaat gtctcattta gtggctggtt acccgaatct ggaaatttct ctcgaagtgg
     3061 cagacgcctt agtgcgtggc ggtagtactt ttctagaaat tcaactcgct tttagtgatc
     3121 caagtgcaga tggcccagca attcaaaccg cctgtgctga cgctctttct aagggctttt
     3181 caactaaaaa agggctcgct tttattgccg aagtacataa aaggcatcct aatatcccct
     3241 tatttttaat gacttatgct tccttagcct atactccagg tattgaaaac tttgtaaaag
     3301 aagcaaagga agcaggtgta tctggagtca ttatccctga tttgcctttt gatcaagatg
     3361 aaggacttac tgcagcatgc caaaaatggg gcgtcgaaaa cattcctgtc gcggctccat
     3421 ccatgactca agagcgtctt aataaaatgc ttgatgcaaa attcacttac atctatgcgg
     3481 cgcttcgagc aggaatcacg ggctcagtaa caacaattga taaccaaacg cttcaatttc
     3541 tagactcctt ttctaaagga tcatctaaaa ttttgggtgg ttttggcatt cgtagtggtt
     3601 ctcaagcaaa acaattagca gatcatgtat atgcaattgt tgcaggttcc gtttttgtaa
     3661 atgaaattac aactcactac gatccaaaaa atgaaattga atctaaaata aaaattggtg
     3721 ccgctgtaga agccaaagca aaagaaatta ctttatctta ataatgatta tctaaaaaag
     3781 aaacccaccg gctaagccgg tggttcttca tttacgggca tagccct
//
LOCUS       DAOOBB010000182         3819 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_26784_length_3819_cov_4.287991, whole genome shotgun sequence.
ACCESSION   DAOOBB010000182 DAOOBB010000000
VERSION     DAOOBB010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26784_length_3819_cov_4.287991"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1962)
                     /locus_tag="PLT31_07875"
     CDS             complement(<1..1962)
                     /locus_tag="PLT31_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 98"
                     /protein_id="HPW95085.1"
                     /translation="MMLKIKNLNFPKKDVFIGALVLVVLVVAFVIFNSLSIPVARSWD
                     IKFGYYAILSTFIILVAGLVVNAKDIIQTIQTHRPSSKSLLILSAVLAFFLLFILANI
                     ENSHRVLSDETSWESMGLQMFFQHSGGVCNEGIWSNGALQCYTEVNNFKGKALAFVYS
                     IVFLFAEPGRDTALLVNLPLYLLSLIFFFFSLSIWFKSEKLALAATAFLGGMPIYLLQ
                     SRSASTEVLYIFLLTALMSWYASISPKQVTWKHFLLTVPLLGFFAQTRQETVFAFIPF
                     ALYYVSYFREKVYRLPLFVVSVIAVSWPSINTMAAYRGYDFQGGEHAAHSLSNFWFNI
                     QSNIEIMLNLKKDSSFGGIMQNPFYTSFTIILLLATVWLLGRLIISKKYKRGALLAIL
                     FSIQIFVILLNVSGTFQIDINQRYVLVALPLFALIMALGLKDFLSTVLKIKSDKSSTL
                     VLILSIVLSVGLAFFHVFSYRMNMLYYKNKLLVEEHYLNTHLKTYPKESVFIYSRPWQ
                     MLASGHSSFSERTFMNWSTEEFLSWMKKTNNNIYIVRGQDGYGEVNRKSRVVGFKTTN
                     QIEIILKEYKTKRLLMESRPFGYPLTIHQLISKKGFSHYTQNMTFSEWNGQFILMKKN
                     FEDSIRYDVMVNDSLNEGKVLSENVDTLWL"
     assembly_gap    2093..2192
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2193..3487)
                     /locus_tag="PLT31_07880"
     CDS             complement(<2193..3487)
                     /locus_tag="PLT31_07880"
                     /inference="COORDINATES: protein motif:HMM:NF012548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine-type phosphatase"
                     /protein_id="HPW95086.1"
                     /translation="MKKCIRFFVFLLLCFTSVQAETTLESLLKDKGKMGSNYYVYSPV
                     FKKQSKVPSGYKPFYISHYGRHGSRYHHTAKDYQYVYETLQQAQKAGALTTLGESVLV
                     RAEALYKEGKDRAGDLTQKGVAQQQEIAKRMYQNFPALFKGDAFIKAYSSTSGRCIMS
                     MGTFLQELKAQNPRLEIYQESSQRLMSFISPFSFDSSKSYMESKEWKEAYDLLYGEVV
                     HPDLLMKSLFKDSAYVSKNINPHDFLAKMYDLQSSLQGMDDVEFDFSDVMTDEIMLGR
                     YQAQNAWWYGVLGAGPLTYAKGPTFAKPLLKHILEDADEIIEADTTQNKNEKGSRKAT
                     ATLRFGHDTALLGLAGLMQLDIARAQVKDLKELYKVWNDFNLISMGANLQMVFYKSAK
                     KGNPILVKFLYNESEVTVPVACAAKNQCPAVPYYRFEDVR"
     gene            complement(3484..>3819)
                     /locus_tag="PLT31_07885"
     CDS             complement(3484..>3819)
                     /locus_tag="PLT31_07885"
                     /inference="COORDINATES: protein motif:HMM:NF023433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95087.1"
                     /translation="ESLVIDFINETDLDTIRDKENVIEAFYAFAQRKQKIEALELITE
                     EDLNVEEAKRYITISLKREYASENGTELNNLLPKMSPLNPLYLIKKQTVFKKLVSFVD
                     KYKGVGGHL"
BASE COUNT         1275 a          762 c          618 g         1064 t
ORIGIN      
        1 tagccacaac gtatctacgt tctcagaaag aaccttccct tcattcaagg aatcattcac
       61 catcacatca taacgaatgg aatcttcaaa atttttcttc attagaataa attgaccatt
      121 ccattcagag aacgtcatat tctgcgtgta atgagaaaat ccttttttag aaatcaactg
      181 atgaatcgtc agtgggtaac caaaaggacg gctttccata agtaaacgct tagtcttata
      241 ttcttttaag atgatctcta tttgatttgt cgtcttaaaa ccaacgactc ttgattttcg
      301 attgacttca ccatagccat cttgcccacg aacaatataa atgttgttat tagtcttttt
      361 catccaagac aaaaattctt ctgtactcca attcataaaa gtacgttcac taaagctact
      421 atgcccagag gcaagcattt gccaagggcg agaatagata aaaacagatt ctttagggta
      481 tgttttcaag tgtgtattaa gataatgttc ttctactaaa agcttgtttt tataatagag
      541 catgttcatg cgataactaa acacatggaa gaacgcgagc cccacagata aaacaatcga
      601 caagattaaa actaaagtgg atgatttatc gctttttatt tttagaactg tagaaaggaa
      661 atcttttaag ccaagggcca tgatcagagc aaataagggc aaggccacta acacataacg
      721 ctggttgata tcgatttgga atgtgccaga gacatttaat aaaatcacaa aaatctgaat
      781 gctaaaaaga atcgccagaa gagcaccacg tttgtatttc ttagaaataa tcaaacgacc
      841 taaaagccaa accgtggcga gaagtaaaat gatcgtaaat gaagtataaa aaggattttg
      901 cataatcccg ccaaaagacg agtccttctt taaattcaac atgatctcaa tattagactg
      961 aatattaaac cagaaattcg agagcgaatg agctgcatgt tctccgccct gaaagtcata
     1021 gcctcggtaa gccgccatcg tattaataga aggccagctc actgcaatca cagaaaccac
     1081 aaataaaggt aaacgataaa ccttttctct aaaataagaa acatagtaga gagcaaaagg
     1141 gataaaggca aaaacggttt cttgacgcgt ctgagcaaag aatcctagca aaggaacggt
     1201 cagtaaaaaa tgtttccaag tcacttgctt agggctaata gaagcatacc aagacattaa
     1261 agccgtaagt aaaaagatat agaggacttc tgttgatgcc gaacgagatt gcaatagata
     1321 gataggcatt ccgcctaaaa aggccgttgc cgcaagagcg agcttctcac ttttaaacca
     1381 tatggataac gagaaaaaga aaaagattaa actcaataaa tagaggggca agttcacaag
     1441 caacgccgta tcacgaccag gctctgcaaa tagaaacaca atagaataaa caaatgcaag
     1501 agccttcccc ttaaagttat tgacttccgt gtaacactga agagccccat tgctccaaat
     1561 accttcatta caaacaccgc cagagtgctg aaaaaacatt tgtaatccca tcgactccca
     1621 actcgtttca tcactcaaca cgcgatgaga attttcaata ttagcgagaa taaaaagcaa
     1681 gaagaacgct aaaaccgccg aaagaattaa taggctttta gacgaaggac gatgagtttg
     1741 aatcgtttgg atgatatcct ttgcattgac cacgagaccc gcaacgagaa tgataaacgt
     1801 agacaatatg gcataataac caaacttaat atcccacgaa cgtgcgactg gaatagaaag
     1861 agaattaaaa atgacaaagg ctacgacaag aacaacaagt actagagcgc caatgaatac
     1921 atctttttta ggaaaattca aatttttgat ttttagcatc ataaccctaa atgtaaaaaa
     1981 ttgtgagttc caccctccac ttctttttat cgtttaacga aataaatcat tttttatcat
     2041 aaaaaaacgt catttactcc ctctacaatt cctcaaaagc cctatttttc tannnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgcacatc ttcaaaacga taataaggca
     2221 ctgctgggca ctgatttttt gcagcacaag ccacagggac agtcacttct gactcattat
     2281 acaagaactt gactaaaata ggattccctt tcttagccga tttgtagaac accatctgaa
     2341 gattcgcacc catagagatt agattaaaat cgttccacac tttatataac tcttttaagt
     2401 ctttaacttg agctcttgca atatctaatt gcattaagcc tgcaagccca aggagagctg
     2461 tatcatggcc aaaacgaaga gtggctgttg cttttctact ccccttttca tttttatttt
     2521 gagtcgtatc ggcttcgatg atctcgtcag catcttctag aatatgcttt agtaaaggct
     2581 ttgcaaaagt aggccctttc gcataagtta acggccctgc cccaagaaca ccataccacc
     2641 acgcattttg agcttgataa cgcccaagca taatttcatc tgtcatcaca tcactgaaat
     2701 caaattctac atcatccatc ccttgaaggc tactctgaag atcatacatc ttagccaaaa
     2761 aatcatgagg atttatgttt ttagaaacat aagcggagtc cttaaaaaga gacttcatca
     2821 gtaaatcagg atgaacgact tcaccataaa gcaaatcata agcctctttc cattctttag
     2881 attccatata actttttgaa gaatcaaaac taaaaggaga aataaaactc atcaaacgtt
     2941 gacttgattc ttgataaatt tctaagcgag gattttgagc ttttaattct tgtaaaaaag
     3001 tgcccatact cataatacaa cggcccgaag tactagagta ggctttaatg aaagcatcgc
     3061 ccttaaaaag agcagggaaa ttttgataca tgcgcttagc aatctcttgc tgttgagcta
     3121 cacccttttg agtcaaatcg cctgctcgat ctttgccttc tttatagaga gcctctgccc
     3181 taactaaaac actttcacca agtgttgtta aagcaccagc tttttgtgct tgctgcaatg
     3241 tttcataaac gtattgataa tcttttgccg tatgatgata acgactccca tgacgcccat
     3301 aatgactaat gtaaaaaggc ttgtaaccgc tgggcacttt tgactgtttt ttaaaaactg
     3361 gagaataaac gtaatagtta ctccccattt ttcctttatc ttttaaaaga gattctagcg
     3421 tggtttcggc ttgcacactt gtaaaacaga gtaataagaa aacaaagaaa cgaatacact
     3481 ttttcataaa tggccaccaa ctcctttata tttatctaca aacgacacta actttttaaa
     3541 aacggtctgc ttcttaatca aatacagagg gtttaaagga ctcatttttg gtaaaagatt
     3601 attcagttct gtgccatttt cacttgcata ttctcgtttt aaggaaattg ttatgtagcg
     3661 ctttgcttcc tctacattta gatcttcttc tgtgattaat tcgagtgctt cgattttctg
     3721 cttgcgttga gcaaatgcat aaaaagcctc aattacattc tctttatctc gaatggtgtc
     3781 aaggtcagtc tcatttataa aatcaataac taaactttc
//
LOCUS       DAOOBB010000183         3780 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_27119_length_3780_cov_4.194094, whole genome shotgun sequence.
ACCESSION   DAOOBB010000183 DAOOBB010000000
VERSION     DAOOBB010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27119_length_3780_cov_4.194094"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="PLT31_07890"
     CDS             <1..775
                     /locus_tag="PLT31_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95088.1"
                     /translation="KNIFITFLFFAFSWGWSYSTAIDTIYHESSDASLPDTIYVIESS
                     ARSELLPIETDKPIYTTYFPINKEVKIKERIFLFIHPVSLFYSLDNKEFLGFVTIEKE
                     MNRIISLIFKPQILFQELSLYDDDWSGTNPYYPNYDREVSLHQTQLKINTGLRIYKRK
                     QQGPFIQVNGGLGFGHCKSIDSEQNKNKFLYATGSMSSTVGRSWKTNHFAIFLDFGVG
                     LKLSSVDYDDNGTFEDVFFVEFGNDFYIDMNFGIGFNIN"
     gene            complement(873..1499)
                     /locus_tag="PLT31_07895"
     CDS             complement(873..1499)
                     /locus_tag="PLT31_07895"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HPW95089.1"
                     /translation="MTLLMDTRIETPDSDWVKEQNSQGFEVLHLPALEVEPLNTILDW
                     SGVEALFLASPRAVFLAQEKLKNFDGPIWVVGEGTAKALTTLGLKATEVGDGSGAFSF
                     LKNLQSRYAGTFPISVLAWISAEETAVDLDFIASQFQLKIRHFPVYRTVFKKYDSSYM
                     NSLSKPKKWFFYSGRAVESFASFISPEDDVVCFGKSAEQKYDQIIKRV"
     gene            complement(1496..2479)
                     /gene="hemC"
                     /locus_tag="PLT31_07900"
     CDS             complement(1496..2479)
                     /gene="hemC"
                     /locus_tag="PLT31_07900"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HPW95090.1"
                     /translation="MEKIWMNKSIIRLGTRQSPLAMAQTKLTATALELFFPDIQIEIK
                     PIVTQGDKDRVRSLVSIGGTGVFVKELEQALIDGEIDVAVHSLKDVPSEMDSRLTLAG
                     FLKRANPHDVLIAKNHTLATLPKGAKVGTGSPRRILQLREQRPDLSFVDLRGNLASRI
                     AKVENNELDAILLGAAGLERLGLLDVVSETFSPDVLTPAIGQGIVGLQCLKKNAEIIS
                     VLERISDPFTAQAARVERAWMSFLGGGCRAPMGAFLAPNGESGSAFYTYLSDPVSKNF
                     YRDVFVQKASVFSESDFELFGEEFIRKCNDLKIPLPKNIDPHRLLDFWGVL"
     gene            complement(2457..>3780)
                     /gene="hemA"
                     /locus_tag="PLT31_07905"
     CDS             complement(2457..>3780)
                     /gene="hemA"
                     /locus_tag="PLT31_07905"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HPW95091.1"
                     /translation="YNFSSLELEIMIKNKEKNHCVVLGVSYKTCPIDIRDNLSRALQN
                     SEEALSSLLGLPGVSEAVVLATCNRAEIYVATLYPEMLRDTLLNWWAEFSKQDLSEVV
                     PYCFYLTHLEAVNHLYTVVSSIDSLVLGENQILAQVRSAFLKAQEAGFTGTLLNHLFQ
                     TALFVGKQVRENTGIGDGTVSIAHAAVQLIEHELGRLENTRIGILGLGEMGLLAATAL
                     SAAEVSSFSFFNRTFEKAKQFASRFGGDAFEIGALTQEIRNIDVLITAVRSQEFILKK
                     KDLASLSKHDLLIVDIASPRNVDPEVGDLDGIRVFCIDDLKEIVEKNRSLRKQASERA
                     RLFIEEAAMDFTEWFYSHSLTPLFVQMQEYHEKIGVFVLNKWKKKVSEEEYAHLERFE
                     EELRKKILHIPFESLKSLSVAGMGLESQLLLEKIYSLTSFKYGKDLDE"
BASE COUNT         1276 a          765 c          605 g         1134 t
ORIGIN      
        1 aaagaacatt ttcattactt tccttttttt tgctttttca tggggttgga gctatagtac
       61 cgctatagat accatatatc acgaatcatc cgacgcttct ctaccagata ctatttacgt
      121 catcgaatct tcagcgagat cagaactctt gccaatagag acagataaac ccatttatac
      181 cacatacttt cctataaaca aagaggttaa aataaaggaa agaatctttt tgtttattca
      241 cccagtttca cttttctata gtttagataa taaggagttt ttgggttttg ttaccataga
      301 aaaagaaatg aatcgaatta tttctttaat ttttaaacct caaattcttt ttcaagaatt
      361 atcgctctat gatgatgatt ggagtggtac aaatccatat tatcctaatt atgataggga
      421 agtttcactt catcaaactc aattaaaaat aaatacaggt ttaaggatct ataagagaaa
      481 acagcagggg ccgttcattc aagtcaatgg tggtttaggg tttggccatt gtaaatccat
      541 tgactcagaa caaaacaaga ataagttttt atatgctact ggctccatgt caagcactgt
      601 aggaagatca tggaaaacaa atcattttgc cattttccta gattttggtg ttggcctaaa
      661 actttcttcc gtcgattatg atgataatgg gacttttgaa gatgttttct ttgtagaatt
      721 tgggaacgat ttttatatcg atatgaattt tggtatcgga tttaatatca attaaagata
      781 cctctaaaac atttcattta ttcaaaagcg gcaaaaatca ttaattctta ccttttttac
      841 atacaccctt atgaaattaa gctaaaaaaa cttcaaaccc gttttatgat ttgatcatat
      901 ttttgctctg cacttttgcc aaaacaaacc acatcgtctt ctggtgaaat aaaagaagca
      961 aacgattcta ctgcacgtcc agaataaaaa aaccattttt taggctttga taaagagttc
     1021 atatatgaag aatcatattt cttaaaaaca gtgcgatata cagggaaatg gcgtattttc
     1081 aattgaaatt gagaagcgat aaaatctaaa tcaaccgcgg tttcctctgc agaaatccaa
     1141 gctaaaacag aaataggaaa agtacccgca taacgagatt gaaggttttt caaaaaagaa
     1201 aaggctcctg atccatctcc tacttcagtt gctttaagac caagtgtggt aagcgctttt
     1261 gctgttcctt cacctacaac ccaaatgggg ccatcaaaat tttttagctt ctcttgagcc
     1321 aaaaaaacag ctcttggact tgctaaaaac aaagcctcca ctccactcca atcaagtatt
     1381 gtatttaatg gttctacttc taaagcaggt aagtgaagta cttcaaaacc ttgagaattt
     1441 tgttctttaa cccaatcaga atcaggcgtt tcgattcttg tatccatgag taaagtcaaa
     1501 gtactcccca aaaatctaat aagcgatgag ggtcaatatt tttaggaaga ggaattttaa
     1561 gatcattaca ttttcttata aattcttcac caaaaagctc aaaatcagat tcactaaaaa
     1621 cgcttgcttt ttgaacaaaa acatcacgat aaaaattctt agaaacaggg tcggataagt
     1681 acgtataaaa agcagaaccc gattctccat ttggagctaa aaaagccccc ataggagcac
     1741 ggcaaccgcc ccctaaaaac gacatccaag cacgttccac tcgagcagcc tgtgctgtaa
     1801 aagggtcaga gatgcgttct aaaacagata tgatttctgc attctttttt aagcactgta
     1861 aacccacaat cccttgacca atcgcaggcg taagaacatc aggagaaaac gtttcagaaa
     1921 caacatcaag taaacctaat cgttcaagcc ccgcagcgcc taagagaatt gcatccaact
     1981 cattattttc caccttagct attcttgaag caagattccc tcttaaatcc acaaaggata
     2041 aatcaggcct ttgttctctt aactgcaaga tacgacgagg agaacccgta cctacttttg
     2101 ctcccttagg gagagttgct aaagtatgat tttttgcaat caaaacatca tgaggattag
     2161 cacgttttaa aaatcctgca agagttaaac gggagtccat ttctgaagga acatctttta
     2221 aagaatgaac ggcaacgtca atctctccat ctataagagc ttgttcaagc tctttcacaa
     2281 aaacgccagt gccaccaatg ctgactaaag aacgcactcg atctttatca ccttgagtaa
     2341 caatcggttt aatttcaatt tgaatatctg gaaaaaataa ttctaacgcc gttgctgtaa
     2401 gctttgtttg tgccatcgcg agcgggcttt gacgtgttcc taaacgaatg attgatttat
     2461 tcatccagat cttttccata tttaaacgaa gttaaggaat atattttttc taatagcaac
     2521 tgactttcta agcccatccc agcgacactt aaggatttga gtgattcaaa agggatatgc
     2581 aatatttttt ttcttagttc ttcttcaaaa cgttctaaat gagcgtattc ttcttcagaa
     2641 accttttttt tccacttatt aagtacaaaa acgcctattt tttcgtgata ttcctgcatt
     2701 tgaacaaaaa gaggggttaa gctgtgagaa taaaaccact ctgtaaaatc cattgccgcc
     2761 tcttcaataa ataaacgagc cctttctgaa gcttgcttac gaagagatct atttttctct
     2821 acaatttctt ttaaatcatc tatacaaaac acacgaatac catcaagatc tcccacttca
     2881 ggatcgacat ttcgaggaga agcgatatcc acaatgagca aatcatgttt tgaaagtgaa
     2941 gccaaatcct tctttttcaa gataaattct tgagaacgaa cagcagtaat gagaacatct
     3001 atatttctta tttcttgtgt tagggcacct atttcaaagg catcccctcc aaatcgagag
     3061 gcaaattgct tcgctttttc aaaagtccta ttaaagaatg aaaaggaaga aacctcagct
     3121 gcagaaagag cggttgctgc taacaaaccc atttctccaa gccctaaaat accaatccga
     3181 gtattttcta aacgccctaa ttcatgttct attaattgaa cagcggcatg agcaatagaa
     3241 acggttccat caccaatacc cgtattttca cgaacctgtt taccaacaaa aagagccgtt
     3301 tgaaacaaat ggtttaaaag tgttcctgta aaacctgcct cttgagcctt caaaaaagcc
     3361 gatcgaactt gagctaagat ttgattttca cctaaaacaa ggctatcaat agaagaaacc
     3421 acggtataaa gatgatttac agcctcaaga tgggttaaat aaaaacagta aggaacgact
     3481 tctgaaagat cttgcttact aaattcagcc caccaattca ataaagtatc ccgaagcatt
     3541 tcaggataaa gggttgccac ataaatttca gcccgattac atgtcgcaag cactacagcc
     3601 tcagaaacac caggtaatcc taataagctg cttaaggctt cctcagaatt ctgcaaagca
     3661 cggcttaaat tatctcgaat atcaattggg caggtcttat aacttacgcc aagaacaaca
     3721 caatgatttt tttccttgtt tttaatcata atctcaagct ctaaactaga aaaattatac
//
LOCUS       DAOOBB010000184         3773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_27192_length_3773_cov_4.204680, whole genome shotgun sequence.
ACCESSION   DAOOBB010000184 DAOOBB010000000
VERSION     DAOOBB010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27192_length_3773_cov_4.204680"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="PLT31_07910"
     CDS             complement(<1..633)
                     /locus_tag="PLT31_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95092.1"
                     /translation="MLKKTLLLTFLICPSLWSFTYDQHGILGLDYGPNISWTFAGNTP
                     ISFGQWLPQFTGSLDYNWIEPLGERLPYGEEIGFREAFLRLQASIQTSPFYGGFMVGM
                     GLRPFKTNPQVEVRFLYQSTIYYNSNIEMVLADSTGGGSISDSWNADYVLDHVWSQEF
                     SRVDFSQNFNVWIDFDFAFRSGSIMGLGGHFTLTDIQTKHDNKSYDYERNL"
     gene            737..1171
                     /locus_tag="PLT31_07915"
     CDS             737..1171
                     /locus_tag="PLT31_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95093.1"
                     /translation="MKMTKWILLGLCSLVFFGCANRYSSPILIERGEGRKEYEREYFA
                     IKESMGIDKRLKDVFKQGYIEEGMTNDMVNLLWGPPDRETNDGRIWEYYTREGKLITR
                     LLWKNPEQARLKGYENELVLESIEGDRYGGSPAPTSKSYSNY"
     gene            1311..2072
                     /gene="tpiA"
                     /locus_tag="PLT31_07920"
     CDS             1311..2072
                     /gene="tpiA"
                     /locus_tag="PLT31_07920"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPW95094.1"
                     /translation="MRQYIIAGNWKMNKTVSESVTLAKQIVEAVKDVKNTEVVIAPTY
                     LAAAKVADVVKGTNVKLAVQDIHWKDQGAFTGKVSIDMVKEIGAEYVIIGHSEQRQYF
                     HETEETVNLKVKKTLSAGLKPIICIGETLEERNGGILENVLTTQIKGAYQDLSADDAL
                     KTVIAYEPVWAIGTGVVATDEQAQDTQAFVRSVMKNIYGESVSEAVRIQYGGSMKPDN
                     AKGLLGQKDIDGGLIGGAALKSDSFFAIIAAAEAQ"
     gene            2097..2504
                     /gene="secG"
                     /locus_tag="PLT31_07925"
     CDS             2097..2504
                     /gene="secG"
                     /locus_tag="PLT31_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HPW95095.1"
                     /translation="MSTLFWIGIVLHVFLCLFLMLLVLIQNDKLGGLAGLGGMTSQSA
                     FSTAGAATFIQKLTRAVAIVFFVVVLALGLVATRQNAGPVVSDLKKATESSSVNETVV
                     PQLPSAIPAAVPALPAEGPVENSTEAPLETNAP"
     gene            2540..2622
                     /locus_tag="PLT31_07930"
     tRNA            2540..2622
                     /locus_tag="PLT31_07930"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2573..2575,aa:Leu,seq:gag)
     gene            3190..>3773
                     /locus_tag="PLT31_07935"
     CDS             3190..>3773
                     /locus_tag="PLT31_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPW95096.1"
                     /translation="MDKKYVYLFSEGKGSMRELLGGKGANLAEMTNLGMPVPQGFTIS
                     TEACTKYYDDGEKISEDLEKSIMEYIEKMEAITGKKFGDLENPLLVSVRSGARASMPG
                     MMDTILNLGLNDSVVVAFAEKTGNPRFAYDSYRRFIQMYSDVVMEVGKKYFEELIDQL
                     KEKKGVKLDTELTADDLKLLADQFKAEYKKKIGS"
BASE COUNT         1221 a          679 c          711 g         1162 t
ORIGIN      
        1 aagatttcgt tcataatcat aacttttatt atcatgtttc gtctgaatat cggtcagtgt
       61 aaaatgccca cctaaaccca taatcgatcc acttctaaat gcaaaatcaa aatcgatcca
      121 aacgttaaaa ttctgtgaaa aatcaactct tgaaaattct tgactccata catgatctaa
      181 gacataatct gcattccaag agtccgaaat tgaaccaccg ccagtagaat cagctaatac
      241 catctctatg ttagaattgt agtatatcgt actttgatat aaaaaacgga cttccacttg
      301 aggattggtc ttaaaaggac gaaggcccat tccaaccata aacccgccat aaaaaggaga
      361 agtttgtatt gatgcttgaa gtcttaaaaa agcttctcta aaaccaattt cttcaccata
      421 aggaagacgt tctccaagag gctctatcca attatagtcc aaggaaccag tgaattgagg
      481 taaccactga ccaaaagaaa tgggagtgtt acccgcaaat gtccaggaaa tgttaggacc
      541 atagtcaagg cctaaaatgc cgtgctgatc ataagtaaac gaccaaagag aagggcatat
      601 caagaaagtt agaagtaaag ttttctttaa cattttattt ttattgttat atttgcgaat
      661 aaattataaa attatcatac tactttcatt ataaattctt attttcccta tatctttacg
      721 cctttaagga gagattatga agatgactaa atggattcta ctggggcttt gtagcttagt
      781 gtttttcggt tgcgcaaaca gatattcgtc gccgatctta attgagcgcg gagaaggcag
      841 aaaagaatat gaacgcgagt actttgccat taaggaaagc atggggattg ataaacgtct
      901 aaaagatgtt tttaagcaag gatatattga agaaggaatg actaacgaca tggtcaattt
      961 actatggggt cctcccgata gagaaacaaa tgatgggcgc atttgggagt attatactcg
     1021 tgaaggcaag ctaatcactc gtttactttg gaaaaatcca gaacaagctc gtctaaaagg
     1081 atacgaaaat gagctcgttt tagagtccat tgaaggagac cgctatggtg gctcccctgc
     1141 accaacctca aaatcctatt ctaactacta attttgcgtt tttttagaag ttctttttaa
     1201 cttcttatca aaacaattaa attctttaaa acaccgtcta gcataggcgg tgtttttttc
     1261 taactttgca ctcgtaaagt tgaaccttaa acctcctgaa ggagttctca atgcgtcagt
     1321 atattatcgc cggcaattgg aaaatgaata aaacagtttc tgaaagtgtt actttagcaa
     1381 agcaaatcgt tgaagctgta aaagatgtga aaaatacaga agttgttata gcccctactt
     1441 atcttgctgc cgcaaaagta gctgatgtgg ttaaaggaac gaacgtaaaa ttagctgttc
     1501 aagatatcca ttggaaagac caaggagcct ttactggtaa agtttccatt gacatggtta
     1561 aagaaattgg tgctgaatat gtgattattg gtcactctga acaacgtcag tattttcatg
     1621 aaacagaaga aactgtgaac ttaaaagtaa agaagacctt aagtgctggt ctaaaaccaa
     1681 tcatctgcat tggtgaaaca ttagaagaaa gaaacggtgg catattagaa aacgtcttaa
     1741 caactcaaat caaaggtgct tatcaagacc tttctgctga tgacgctctt aagacagtca
     1801 ttgcatacga acctgtatgg gctattggta caggcgttgt cgctaccgat gaacaagctc
     1861 aagatactca agcttttgta cgttctgtaa tgaaaaacat ttatggcgag tctgtttctg
     1921 aagccgttcg tattcaatac ggtggatcca tgaaaccaga caatgcaaaa ggtcttctcg
     1981 gtcagaagga cattgatggg ggtctcattg gtggtgccgc cttgaaatca gactctttct
     2041 ttgctatcat tgccgctgct gaagctcaat aattcatcgt tttcaaggaa ttaaaaatgt
     2101 cgacactttt ttggatcgga attgtattgc atgtatttct ttgcttattc ctaatgttac
     2161 tcgttcttat tcaaaacgat aaattaggtg gattagcagg tctcggaggc atgacttctc
     2221 aatctgcttt ttcaacagct ggagctgcta catttattca aaagttaaca cgcgctgtag
     2281 cgattgtttt ctttgttgtt gttctcgctc ttggtttagt agcaacaaga caaaatgcag
     2341 gaccagttgt ttccgatctg aaaaaagcga cagaatcttc tagtgtaaat gaaactgttg
     2401 ttcctcaatt gccatcagca attccagcag cagtacctgc attaccagct gaaggtcctg
     2461 tggaaaattc tacagaagcc cccttggaaa ctaatgcgcc ctaatctata tttgcacgcg
     2521 tagaaaaagt ttaccccacg cggtcgtggt ggaatggtag acacgctagc ttgaggtgct
     2581 agtgggagca atcctgtgac agttcaagtc tgtccgaccg cactgaaaaa cttcgatgaa
     2641 agtcgaagtt ttttttattt taaactgttt atatttcatt attttttctt ttattttttg
     2701 ctttttatct ttatcctttg caccagttta tttaggatta tttgattata agccattgca
     2761 tacaaagtat tttatataaa taacgatacc ccatttttta tccatattgt ataagctctt
     2821 ttagaaatga acgtcccaat tatttctttc tttatttagg ttctattaag gtaataaaac
     2881 attttttgat cctatttatt tcgctgtaaa ttgcaaaaca ccaacaacga gcataaaagt
     2941 ttttctcacc ttccttccct tttcaagtaa acatctccct cctcatgatt ttaatgttgt
     3001 caaatcgacg gcagaatgaa ccagattatt tgttactata aattaaatta tcatatttat
     3061 tgaatatcat aaaagacata ataatattta aattcaataa ttcagccacc aattaaagca
     3121 cctttgtaaa ctttttgcta catttgtatg taacatataa aaaaagaaga aaaaaggaag
     3181 tctatgaaca tggacaaaaa gtacgtttat ttattttctg aaggaaaagg ttccatgcgc
     3241 gaactcctcg gaggaaaggg cgcgaatctt gcagaaatga ccaatcttgg aatgcctgta
     3301 ccacagggtt ttacaatttc aactgaagcc tgtacaaagt attacgatga tggtgaaaaa
     3361 ataagcgaag acttagaaaa atcaattatg gaatacatcg aaaagatgga agccattact
     3421 gggaaaaaat ttggtgatct agagaatccc ctcttagtgt ctgtgcgttc tggcgcacgc
     3481 gcttctatgc ctgggatgat ggatacgata ttgaatcttg gtttaaatga ttctgtcgtt
     3541 gtggcttttg cagaaaaaac tggaaatcca cgttttgctt acgattcgta tcgccgtttt
     3601 attcaaatgt attccgacgt tgttatggaa gtcggcaaaa aatatttcga agaactgatc
     3661 gatcaattga aagagaagaa aggcgttaag ttagacactg aactcactgc agatgatctg
     3721 aaacttttag ctgatcaatt taaggcagaa tacaagaaaa aaattggaag cga
//
LOCUS       DAOOBB010000185         3709 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_27788_length_3709_cov_4.219759, whole genome shotgun sequence.
ACCESSION   DAOOBB010000185 DAOOBB010000000
VERSION     DAOOBB010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27788_length_3709_cov_4.219759"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..611)
                     /gene="mscL"
                     /locus_tag="PLT31_07940"
     CDS             complement(135..611)
                     /gene="mscL"
                     /locus_tag="PLT31_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HPW95097.1"
                     /translation="MDIKGQFKGKASSLMDEFKSFAFKGNIVDMAVGVIIGGAFGKIV
                     TSMVNHIIMPFVSLIMPSGTNYADWKLVVNRGTEIVDGVEKAKMIEIPYGLFLSEIVS
                     FLIIAIVLFFVIKKFLGFMMNMKKKDEPAPAPAEPPPPPVEQVLLTEIRDLLKEKR"
     gene            686..1561
                     /locus_tag="PLT31_07945"
     CDS             686..1561
                     /locus_tag="PLT31_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPW95098.1"
                     /translation="MIWILLAFSSAFFLGFYDISKKKALSHNAVWPVLFFCSFTTVIC
                     LFPFFLMGKIPLIEAESQLPIFLKALIVTSSWAFTFNAVARLPLSITAPIRASAPLFT
                     ILMAVSFMGERPSVYQWLGIGITAGSYFLFSIAGKKESGSFFRNPWVIAMFFGSFLGA
                     CSGVYDKYLLQHLKYDPLTVQFWFNLYMSAIQLGIIGFLVLKKKTSSGRPFRFHPIMI
                     LVGFLLLIADRFYFLALHEPDALVSVVTVIRRSSVIISFLWGILIIKEHKSKTKYIGF
                     MGIILGLLFITLSKG"
     gene            complement(1606..1678)
                     /locus_tag="PLT31_07950"
     tRNA            complement(1606..1678)
                     /locus_tag="PLT31_07950"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1642..1644),aa:Glu,seq:ttc)
     gene            1755..3302
                     /locus_tag="PLT31_07955"
     CDS             1755..3302
                     /locus_tag="PLT31_07955"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW95099.1"
                     /translation="MNLQILLKKTIFKAFFLLSGPAFLASLFDASLLAGIRFFVEILS
                     ETSPFTITEWTLAMILLICLRFIFLRSRVVTTQKIFRQLESGLQGYFLSILRHIQPRF
                     FHHPESDSKVQAAFEATRTIPLSGEALMQTLQAVLQLLIFIPVLFYLSWPLSLLLFVV
                     VMPVLAYTQNKWKTIGPIIEKQMNSEALFRSELEQIKKLYRFWSSAIEKSIETNRLIQ
                     LIRSRRTISVEAGIKQASLSFMIESVSILAMIGVLTFCGWMISKSWMEPKDLILYCSA
                     VFLCYKPVKECIRIFPQLRAAKTAYNILSRFAEEPRQKREVKTYGSALKITSASFRYE
                     NGVKPIFNLFSTEWEINEPVLITGPNGAGKSTLLRLIAGLEEWHSGIISYPNIYNKAG
                     IFFISQNIVLPPIYLLNHLIKQNENPYLNDFIEQANVSHLFSSNSFSGGEKARFALIW
                     ALASKSQILLLDEPFAYISRFDKDSLLNNFLNTAKVLNKWIILSSHEKMEESMLKRFQ
                     QVIIDGK"
     gene            3292..>3709
                     /locus_tag="PLT31_07960"
     CDS             3292..>3709
                     /locus_tag="PLT31_07960"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPW95100.1"
                     /translation="MESKLYQYRGWILGLFAVLLLVLPPTTSFFSLSAILLIFSGILI
                     RIEARRSIGEHSRGHSLSADALVKTGIYAYLRHPLYLSNIMIGCGFILIHLSWTSTSF
                     ILATALILFFYNLSVQEDRFLLNQFGESWLAWKDKTN"
BASE COUNT         1058 a          749 c          644 g         1258 t
ORIGIN      
        1 atccttctaa agcctactcc ctcatgggtt ggaaagccaa gtactctgat ttagaaactc
       61 taattaaaac cacttggaaa gcttatcaat cacaataaaa aacacccctt ttttaggggt
      121 gccttattaa atcattagcg tttttctttt aagagatctc ggatttctgt gagtaaaact
      181 tgttctactg gaggtggagg aggttctgca ggagcaggtg ctggttcgtc ttttttcttc
      241 atgttcatca taaaaccaag aaacttttta atcacaaaga atagcacaat agcaatgatt
      301 aagaagctta caatttcact taagaaaagg ccgtaaggga tttctatcat ctttgctttt
      361 tctacaccat cgacgatttc cgttccacga ttcacaacta gtttccagtc ggcataatta
      421 gtgccgctag gcataattaa tgatacgaat ggcatgatga tatgatttac cattgaggtg
      481 actatttttc caaaagcgcc tccaatgata acacctactg ccatatcgac aatattacct
      541 ttaaatgcaa atgatttgaa ttcgtccata aggctgcttg ccttaccctt aaactgacct
      601 tttatgtcca tgttgatctc ctgtttaaat gagaacaaat taatattatt tttaaagaaa
      661 agtgaaacta agctatcttt atggcatgat ttggattttg cttgctttta gttctgcttt
      721 tttcctcggt ttttacgaca tcagcaaaaa aaaggcctta tcccataatg cggtatggcc
      781 tgttcttttt ttctgtagct ttaccactgt aatttgtcta tttccattct ttttaatggg
      841 aaaaattccc ctaattgaag cagaatcgca acttcccatc tttttaaaag cgttaatagt
      901 gacaagcagc tgggctttta cctttaatgc cgtggctagg cttcccttaa gtataaccgc
      961 ccccattcga gcaagtgccc cattatttac cattttaatg gctgtatcat ttatggggga
     1021 acgcccctct gtttatcaat ggttaggaat aggaatcact gcaggaagct actttttatt
     1081 tagcatcgca gggaaaaaag aatctggttc tttttttaga aatccttggg tcatcgccat
     1141 gttttttggc tcgtttctag gagcctgtag cggtgtttat gacaaatatc ttcttcagca
     1201 cctaaagtat gatcctctca cagtgcagtt ttggtttaac ctttacatga gtgcaattca
     1261 gcttggaatc atcggttttt tagtcctcaa aaaaaagact tccagcggac gtccttttcg
     1321 atttcatcca atcatgattt tagtgggctt tttactttta attgccgata ggttttattt
     1381 tttagctcta cacgaacccg acgctcttgt ctctgtagta acggtaatac gtcgttcaag
     1441 cgtcattatt agcttcctct ggggaattct tatcataaaa gaacataaaa gcaaaacaaa
     1501 atacattggt tttatgggta ttattttagg gcttttattt atcactttaa gtaaaggcta
     1561 gctctaaaaa taaaaaagcc ctatcaaaag atagagcttt ttaagtacct ccaaggggtt
     1621 tcgaacccct gttgcgggaa tgaaaatccc gagtcctggg ccactagacg atggaggcat
     1681 ctaggtaggg ccaaatatag gaaagaatta tctcttgtaa aggctcccct tttaaaaaag
     1741 ctattttata agccgtgaat ttacagatct tattaaagaa aaccattttt aaagcctttt
     1801 tcctgttatc agggccagct ttcttggcta gtctttttga tgctagtcta cttgcaggta
     1861 tccgtttttt tgtggaaatc ctttcagaaa cgtctccttt tacgattact gaatggacat
     1921 tagcaatgat tttactcatt tgcttacgct ttattttttt gcgttctaga gtagtcacca
     1981 cccaaaagat attccgtcag ctggaatctg gcctacaagg gtatttttta tctattctca
     2041 ggcatattca gccccgtttt tttcaccacc ctgaaagcga ttcaaaagta caagccgctt
     2101 ttgaagccac tcgaacgatt cccttaagtg gtgaggcctt aatgcaaacg ctccaggccg
     2161 ttcttcagct cctcattttt atacccgtct tattctattt atcatggcct ttatcgcttc
     2221 ttttattcgt tgttgtgatg cctgttcttg cttataccca aaataaatgg aaaacaattg
     2281 gtcctattat tgaaaaacaa atgaatagcg aggctttatt tcggtcggaa ctggaacaga
     2341 ttaagaagtt ataccgtttt tggagttcag cgatcgagaa gtccattgaa accaatcgtt
     2401 taattcaatt gatccgttct cgacgtacca taagtgtaga agcaggaatc aagcaggcct
     2461 ctttatcctt tatgatcgaa agcgtatcca tactcgcgat gatcggtgtt cttacgtttt
     2521 gcggttggat gatttcaaaa tcttggatgg aacccaaaga ccttatcctt tattgttccg
     2581 cagtctttct ctgttataaa cctgtaaaag aatgcatccg tatttttcct caattacgtg
     2641 cagcaaaaac ggcgtacaat attttgagtc gttttgcaga agagcctcgt caaaaaagag
     2701 aagtcaaaac ttatggctcc gccttaaaaa tcacaagcgc ctcttttaga tatgaaaacg
     2761 gagtcaagcc catctttaac ttgttttcta ctgaatggga aatcaacgaa cccgttctca
     2821 ttacagggcc aaatggcgct gggaaaagca cattactccg attaatcgcg ggccttgaag
     2881 aatggcattc tggcatcatc tcttatccaa atatttataa caaagcggga atatttttta
     2941 tttcacaaaa catcgtatta cctcccattt atctcctcaa ccatcttata aaacaaaacg
     3001 aaaatcccta tttaaatgac tttatagaac aagctaatgt aagccacctt ttctcctcta
     3061 attccttttc tggaggtgaa aaagcccgtt tcgcattaat ttgggccttg gcctctaaaa
     3121 gccaaattct tttattagat gaaccttttg catacatttc tcgatttgac aaagattctt
     3181 tattgaataa ttttttaaat acggcaaaag ttttaaataa atggattata ttatcaagtc
     3241 atgaaaaaat ggaagaatct atgctcaaac gttttcaaca ggtgattatt gatggaaagt
     3301 aaactctatc aataccgagg atggattctt gggcttttcg ccgtattatt attagtgctt
     3361 ccaccaacaa catctttttt ttcactctct gccatccttc tcattttttc tggaattctt
     3421 attcgaattg aagcgagaag aagtattgga gaacacagca gagggcactc cttatctgca
     3481 gatgctttag taaaaacagg tatttacgcc tatttacgtc accctcttta cctttctaat
     3541 atcatgatcg ggtgtggttt tatacttatc cacttaagtt ggacttcaac ctcctttata
     3601 ttagcaacag ctcttatttt attcttttac aacctttctg tacaagaaga tcgtttttta
     3661 ttaaatcaat ttggagaatc ttggttagct tggaaagaca aaaccaatg
//
LOCUS       DAOOBB010000186         3700 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_27869_length_3700_cov_4.052401, whole genome shotgun sequence.
ACCESSION   DAOOBB010000186 DAOOBB010000000
VERSION     DAOOBB010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27869_length_3700_cov_4.052401"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /gene="prfC"
                     /locus_tag="PLT31_07965"
     CDS             <1..283
                     /gene="prfC"
                     /locus_tag="PLT31_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547303.1"
                     /note="stimulates the release of release factors 1 and 2
                     from the ribosome after hydrolysis of the ester bond in
                     peptidyl-tRNA has occurred; GDP/GTP-binding protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HPW95101.1"
                     /translation="PIIGVVGELQFDVMQFRLSSEYGAEVQLDRIPAYGIRWVMGPET
                     AVQSFASEYAMDCMFDKENRLVCLFPNVYRLNLATKNYETLTFSKTSAT"
     gene            complement(477..887)
                     /locus_tag="PLT31_07970"
     CDS             complement(477..887)
                     /locus_tag="PLT31_07970"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HPW95102.1"
                     /translation="MSSIDYLKSISLIHENDNREVFRTCPLLEKAEEQLHEYFSRQRQ
                     NFEIPIDFHGSEFQEKVWKAVQEIPYGHTKTYQEIAQFIGNEKASRAVGLALNKNPLP
                     FIIPCHRVILKSGISGGYAFGNRLKQLLLEQESL"
     gene            complement(1023..1607)
                     /locus_tag="PLT31_07975"
     CDS             complement(1023..1607)
                     /locus_tag="PLT31_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95103.1"
                     /translation="MKFFWGFLLALLFFIEPSMAQDAVAEGSVAQDSLVLDNAVTDSS
                     VLDRPKFPLLVPYGIEIGEGTSFVLGLEWWSLHFSPAIYMKHKPDDKEGMWPPIYALV
                     WSIRTQYVYDYTDHESGFLLYPNIQYLKFFLGVAVGPKVGWFSSSGFDYGASFRLDIF
                     MFFDIEVGYLVNKENMFVTFTFDLSLPRYSLFDP"
     gene            complement(1712..2014)
                     /locus_tag="PLT31_07980"
     CDS             complement(1712..2014)
                     /locus_tag="PLT31_07980"
                     /inference="COORDINATES: protein motif:HMM:NF016136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HPW95104.1"
                     /translation="METTVIQTRIDSNLKKEADDFFQSIGLDMTTAIRLFLNQVLIQR
                     KIPFEIISKDTITRQSALENFYALRAESKNLQEMSLDEINAEIQSIRERSKGKAYK"
     gene            complement(2276..>3700)
                     /locus_tag="PLT31_07985"
     CDS             complement(2276..>3700)
                     /locus_tag="PLT31_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HPW95105.1"
                     /translation="LFDLTNVISLDFKPSGEGTSGNFQVDNIRLTNKRELPKVERPAV
                     VKGTIKGNFNEVINPEIAGGIFGINAALWDGDLLSARGAKTQTADYVKRVNHGIIRYP
                     GGLRADDDHWKEILDNKDWMVDTDEFLEWLKKTGANAMFTVNFGSGTAEEAAAWVKHT
                     NVTKKANIKYWEIGNEVYGNWHPYYEKYGKDGGDIYGKRAREFILAMKKVDPTIKVAV
                     LGVLEGEWNEKVLKHTADVADGIIVHHYPQHFGEENDFALLSSPQTLDKIYERLHQVV
                     KKHSKNKDKQLEIWLTEWNSVDFNPGVQTLSIVNGLFVADYLGMLAQVGADNAQYWDI
                     HNDLTPEGGDYGYLTRSYEEGCGNCPRPSYWAFKMASDALRGKLVKTTVEGDKEAMIT
                     TYLTQNGKKKSLLVVNKSPYSDFDFKLDIPEFKGKAKMQVLDASTEKLKPGFENDPEK
                     KAKAIDVTKGVKVPKRSLVLITVE"
BASE COUNT         1133 a          734 c          660 g         1173 t
ORIGIN      
        1 acctattatt ggggttgttg gcgagcttca atttgatgtg atgcaattcc gtttgtcttc
       61 agaatacgga gctgaagtcc aattagaccg aattcctgct tatggcattc gatgggtaat
      121 gggcccagaa accgctgttc aatctttcgc tagtgaatat gccatggact gtatgtttga
      181 caaagaaaac cgcttagttt gcttattccc aaatgtatat cgattgaatt tagcaaccaa
      241 aaattacgag actttgacct ttagtaaaac gtctgcaact taatttttat tattgctcat
      301 acgttttttg ggctagttcc aattagcttt tcttacgttt ttaaagtcca ttttggaaca
      361 aagtacattt gcaagtaatc agaatatttt acccgaaatg ttttttattc ctgataaatt
      421 tattgtcacc acaaaatggt aagtcattca aaaatatatg agttatggcg ttataattac
      481 aatgattctt gttctaataa taactgcttg agtctgtttc caaaagcata accgccactg
      541 attccgctct ttaaaatcac tcgatggcat gggataataa aagggagagg gttcttattt
      601 aaggcaagcc caaccgcacg agacgctttt tcgttaccta tgaattgagc tatttcttga
      661 tatgtttttg tatggccata ggggatttct tgaaccgctt tccacacttt ttcttgaaac
      721 tctgatccat gaaaatctat gggaatttca aagttttgcc tttgtcttga gaaatactca
      781 tgcaattgtt cttcggcttt ttctaaaagc gggcaagtac gaaaaacctc tctattatca
      841 ttttcatgga ttaaagaaat cgatttcagg taatctattg aactcacgag taaaaaagaa
      901 ccgataggaa aatttacttt tttaaagaat aaatcgctat ttgtcataga ataggggtga
      961 tttgctagat cattaaaata cttgattaaa gttatacaag cgagtttaga ttcgtcattg
     1021 tgctagggat cgaataagct atatcttgga agggataagt caaatgtgaa cgttacaaac
     1081 atattttcct tattgacgag ataacccacc tcaatatcaa aaaacataaa tatgtcaaga
     1141 cgaaaggaag caccgtaatc aaagccactg gaactaaacc aacccacctt gggtcccact
     1201 gcaacaccca aaaaaaattt cagatactga atattgggat acaaaagaaa cccgctttcg
     1261 tgatctgtat aatcatagac atattgtgtg cgaatggacc ataccaaagc gtaaatggga
     1321 ggccacattc cttccttatc atctggcttg tgcttcatgt agatggcggg actgaaatga
     1381 agcgaccacc attcaaggcc cagcacaaac gaagttcctt cacctatttc aataccatac
     1441 ggtacaagga gtggaaattt cggacgatct aaaacagacg aatccgtcac cgcattatca
     1501 agaacaagag aatcttgtgc aacagaaccc tctgctacag catcttgcgc catcgaaggt
     1561 tcaataaaaa acaacaaagc gagaagaaaa ccccaaaaga acttcataat gggaagtata
     1621 gattattttt tatgattcta attccataat tacaagcatt tccgctgacg ttacgatatt
     1681 cgaccttcaa gcattgcatg cagcacaaga atcacttata cgccttgccc ttgcttcttt
     1741 ctctgataga ctgaatttca gcattgattt cgtcaagaga catctcctgc aagtttttgc
     1801 tctcggcacg aagtgcgtaa aaattttcaa gagcactttg tcttgtaatc gtatctttcg
     1861 aaatgatttc aaaaggaatt tttctttgaa tcagaacctg gttcaagaat agcctaatgg
     1921 ctgtagtcat atccaagcca atagactgaa agaaatcgtc tgcttccttt tttagattgg
     1981 agtcaatacg agtttgaata actgtcgttt ccatatttgc acctcaatac aaatataata
     2041 caaaagtatt tgataagcaa tagtttatag gtttataagt agtgttgtat acctatgatg
     2101 tataaattct gcccaattgt attgctttag attgatccac aggttttcgt tgcaaattcc
     2161 gaaatttttg agtgaaaaat tatacagaag tgtcaaaaaa atcacttgac tgcaaaggga
     2221 aaataaaaaa agagctcgct gattaggcga gccctttttc ataagttaga ataaattatt
     2281 ctacagtgat aagaacgagt gaacgcttag gaacttttac tcccttagtc acatcaatcg
     2341 ccttagcttt cttttcagga tcgttttcaa agcctggttt taacttttca gtagaagcat
     2401 ccaaaacttg cattttggct tttcccttaa attcaggaat atctaattta aagtcaaaat
     2461 cactataagg gctcttattg actactaata aggatttctt tttcccattt tgagtcaaat
     2521 aagtggtgat catagcttct ttatcgcctt ccactgtggt ctttacaagt ttaccacgaa
     2581 gagcatcgga agccatctta aatgcccaat agcttggacg aggacagttg ccgcagcctt
     2641 cttcatagga acgagttaaa taaccatagt caccgccttc tggtgttaaa tcgttatgaa
     2701 tatcccaata ttgtgcgtta tctgcaccta cttgagctaa cataccgaga taatcagcca
     2761 caaaaaggcc gttcacaata gaaagagttt gtacgccagg gttgaaatcc acagagttcc
     2821 attcggtcaa ccagatttct agttgtttat ctttattctt gctgtgtttc tttaccactt
     2881 ggtgtaaacg ttcataaatc ttatctaacg tttgtggaga agataataga gcgaagtcgt
     2941 tttcttcacc gaaatgttga gggtaatggt gaacaataat accatcagcc acgtcagctg
     3001 tgtgctttaa tactttttca ttccattcac cttcaagaac accgagaacg gcaactttaa
     3061 tggttggatc tacttttttc attgcaagaa tgaattcgcg agcgcgttta ccgtagatgt
     3121 caccaccatc tttaccatat ttttcatagt aaggatgcca gtttccatag acttcgtttc
     3181 caatttccca atacttgata ttggctttct tggttacgtt agtgtgttta acccaagcag
     3241 cagcttcttc tgcagtaccg ctaccgaagt taactgtaaa catggcgtta gcaccagttt
     3301 tctttaacca ttcaaggaat tcatctgtat cgaccatcca gtctttatta tcgagaattt
     3361 ctttccaatg gtcatcgtct gcacgtaaac caccaggata acgaataatt ccatggttaa
     3421 ctcgttttac ataatctgcg gtttgagttt ttgcaccacg agcggataat aaatcgccat
     3481 cccaaagagc ggcgttgata ccaaagatac caccagcaat ttctgggtta atcacttcat
     3541 taaagttacc cttaatagtg cctttaacta cagcaggacg ttccacttta ggtaattcgc
     3601 gtttgttagt taagcgaatg ttatctactt ggaagttacc acttgtacct tcaccacttg
     3661 gcttaaagtc taagctgatc acgttagtaa gatcaaatag
//
LOCUS       DAOOBB010000187         3674 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_28127_length_3674_cov_4.992816, whole genome shotgun sequence.
ACCESSION   DAOOBB010000187 DAOOBB010000000
VERSION     DAOOBB010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28127_length_3674_cov_4.992816"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(353..910)
                     /gene="rfbC"
                     /locus_tag="PLT31_07990"
     CDS             complement(353..910)
                     /gene="rfbC"
                     /locus_tag="PLT31_07990"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HPW95106.1"
                     /translation="MNKFKFISTPIADLTIVEPTVFGDHRGYFMETFSAHEFKEAGLP
                     TVFVQDNESHSKQGVVRGLHFQKKNPQGKLVRVIEGEVFDVGVDLRRNSPTFGKWFGV
                     ILSAENKKQFYVPEGFAHGFAVLSEMATFVYKCTRLYDPTDEQGLLWNDPEIGIEWPI
                     SKDIPLLLSEKDQNNPTLKNLDFAF"
     gene            complement(910..1938)
                     /gene="rfbB"
                     /locus_tag="PLT31_07995"
     CDS             complement(910..1938)
                     /gene="rfbB"
                     /locus_tag="PLT31_07995"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HPW95107.1"
                     /translation="MSKKNIIVTGGAGFIGGNFVHYMLEKYADYRIVCVDALTYAGNL
                     DTLSSVMNNPNFRFVKMDITDRDSIFKLFQEEHPDIVVNFAAESHVDRSIKDPGIFLK
                     TNILGTQVLMDACREFGIERYHQVSTDEVYGDLPLDRPDLFFTESTPIHTSSPYSASK
                     ASADLLVLAYHRTFKLPITISRCSNNYGPYHFPEKLIPLMIANALADKPLPVYGDGLN
                     VRDWLYVKDHCLAIDLIIHQGRVGEVYNVGGHNEMTNIDIVKLILKALDKPESLITYV
                     KDRAGHDRRYAIDPAKIHQELGFLPTTRFADGIKMTIQWYLENKGWWEKILSGEYQNY
                     YEKMYGDL"
     gene            complement(1959..2846)
                     /gene="rfbA"
                     /locus_tag="PLT31_08000"
     CDS             complement(1959..2846)
                     /gene="rfbA"
                     /locus_tag="PLT31_08000"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HPW95108.1"
                     /translation="MKGIILAGGSGTRLYPLTKVTSKQLLPVYDKPMIYYPLSTLMLA
                     GIRDILIISTPEDLPNFERLLGDGSCMGVRLSYQVQPSPDGLAQAFILGESFIGNDAC
                     AMVLGDNIFYGNGLSPLLKDAVKNAEQNKRASIFGYFVEDPERFGVVDFNEKGQVISV
                     EEKPSHPKSNYAVTGLYFYDNRVVEEAKKQKPSARGELEITDLNRVFLEKGDLDVKLL
                     GRGFAWLDTGTMESLLDAANFVQMIEKRQGIKIAAVEEIAFVNGWISREKLMESAQRY
                     GKSTYGAHLKKVAEGKIRY"
     gene            complement(2907..3425)
                     /locus_tag="PLT31_08005"
     CDS             complement(2907..3425)
                     /locus_tag="PLT31_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95109.1"
                     /translation="MTKIYQASSLRKYNTSVPVFCSNANNSWLGKGYYFWEAFIEVAH
                     WWGKTHYYGSYCIYEGDFNLTTPNGLDLSRGEDIQLLSGVVNNLKERQIKRTITVAQA
                     IAALNVIFNNQFTWVRADTTGCSNELTNSIPYGNHSISLSLTPQIQICFLNEKDFKDF
                     FRSLTKVHPNQS"
     gene            complement(3418..>3674)
                     /locus_tag="PLT31_08010"
     CDS             complement(3418..>3674)
                     /locus_tag="PLT31_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95110.1"
                     /translation="IRNKKFEEAFCSISMIGNFQFNEDITKESIPDYFFQNSIAILYP
                     YIRAFISLLSTQSQSQHILLPTFNLSSLGTILKNKLFEDD"
BASE COUNT         1178 a          729 c          682 g         1085 t
ORIGIN      
        1 acttcgctat tgaattggcc aattcaatta acgcttgttt tttcattaaa aaaacggggt
       61 tgggtttgag atgggcgtct tagcaccttt gcgagattga acacgaaata aaaaaaagac
      121 tgcgatcttt cgcagtccat catttgaaaa aataattttt agaaaatcag tggtgtccta
      181 aatgactgtg ataaaaacgc ttgcatttta caatcaaaag cagagccaag atttcattga
      241 cgtataaaaa aagtgtcgta taaatccttg cattttacaa tcaaaatcaa agccaagatt
      301 tcattgactt gttaaatgac tgtgataaaa acgcttgcat tttaacggca gcttaaaatg
      361 caaaatccaa gtttttaagc gttggattat tttgatcttt ttcgctcaac aacaaaggga
      421 tatctttaga aataggccat tcaatgccaa tctcaggatc gttccacaaa agtccttgtt
      481 catctgtagg gtcgtataag cgagtacatt tataaacaaa agtagccatc tcgctcaaaa
      541 cggcaaaccc gtgagcaaag ccttcgggca cgtaaaattg cttcttgttt tcagcactca
      601 aaataacgcc aaaccatttt ccaaaagtag ggctattacg ccttaaatcc acacccacat
      661 caaaaacttc gccttcgatt acgcgaacga gtttcccttg aggatttttc ttttgaaaat
      721 gaaggccacg aacaacaccc tgcttagaat gagattcgtt gtcttgcaca aaaacagtcg
      781 gaagcccagc ttccttaaat tcatgagcac taaatgtctc cataaagtag ccacgatgat
      841 ccccaaacac agtgggttcc acaatcgtta agtctgcaat aggagtagag atgaatttga
      901 atttattcat tataaatccc catacatttt ttcgtaataa ttttgatact cgccgctcaa
      961 gattttttcc caccagcctt tgttttctaa ataccactgg atagtcatct taatgccatc
     1021 ggcaaaacgg gtggtaggta aaaagccaag ttcttggtga atcttcgctg ggtcaatcgc
     1081 ataacgccta tcgtggcctg cgcggtcttt cacataagta attaaagatt ctggtttgtc
     1141 gagagccttt aatataagct ttacaatatc aatgtttgtc atctcattat gaccgcccac
     1201 attgtaaact tcgcccacgc gcccttgatg aataatcaaa tcaatagcca agcaatggtc
     1261 tttaacataa agccaatcgc gcacgtttaa cccatcgcca taaacaggga gaggtttatc
     1321 tgcaagagca tttgcaatca tcaacgggat caacttttca gggaaatgat aaggcccgta
     1381 attgttactg caacggctaa tcgtaatagg gagtttaaaa gtacggtggt acgcgagcac
     1441 caacaaatca gcagaagctt tacttgcaga ataagggcta gaagtatgaa taggcgtcga
     1501 ttctgtaaag aacaagtctg ggcgatctaa aggcaaatca ccgtagacct catctgtaga
     1561 cacttgatga tagcgttcaa taccaaattc acggcaagca tccattaaaa cttgagtgcc
     1621 taaaatattt gtttttaaga agataccagg gtctttaatc gagcgatcca catggctttc
     1681 ggcggcaaag ttcaccacaa tatcagggtg ttcttcttga aataatttga aaatggagtc
     1741 gcgatcggtg atgtccattt ttacaaaacg gaagttagga ttattcatca cagagctaag
     1801 agtatctagg ttgccagcgt aggttaaggc atccacacat acaatacggt aatcagcata
     1861 tttttcgagc atgtagtgga caaagtttcc gccaataaaa cctgctcctc cggtgacgat
     1921 gatattcttt ttagacataa ataaccttga gacccttatt aataacgaat tttgccttca
     1981 gcgacttttt taaggtgagc cccataagta gacttaccat acctttgtgc cgactccatt
     2041 aatttttcac gagagatcca cccattgaca aaggcaatct cttcaacagc tgcaatctta
     2101 atgccttgac gcttttcaat catctgcaca aagttcgcgg catcgagtaa actttccata
     2161 gtgcctgtat ctagccaagc aaagccacgg cctaaaagtt ttacatcgag atcgcctttt
     2221 tctaagaaga ccctatttaa atcggtgatt tcgagttcgc ctctagcaga aggcttttgt
     2281 ttcttagctt cttcgactac gcgattatca taaaaataaa ggcctgtgac cgcataatta
     2341 ctcttagggt gggatggctt ttcttctaca gaaatcactt gccctttttc attaaagtcg
     2401 acaacgccaa agcgttctgg gtcttccaca aaatagccaa agatactagc tcttttattt
     2461 tgttctgcat ttttaacagc gtcttttaat aaaggcgaaa gcccgttgcc gtagaagatg
     2521 ttgtctccaa gcaccatagc acaagcatcg tttccaataa acgattcgcc tagaataaag
     2581 gcctgtgcta aaccgtctgg gctaggttgc acctgataag aaagccgaac acccatacaa
     2641 gagccatcgc ctaaaaggcg ttcaaaatta ggcaaatctt ctggggtaga aataatgaga
     2701 atgtctctaa tccctgcaag cattagggta gaaagagggt agtagatcat tggtttgtcg
     2761 taaacaggca aaagctgctt actagtgact tttgttaagg ggtaaagtct cgttcccgag
     2821 cccccggcaa gaataatacc tttcatggcc ataaatatat caaaaaataa aaagcaaaag
     2881 agttaatttt tatcaaatga atgtatttag ctttgatttg gatgcacttt agttagtgaa
     2941 cgaaaaaaat ccttaaagtc tttttcattt aaaaagcaaa tttgaatttg aggtgttaaa
     3001 gacagactaa tagagtgatt cccataggga atggagttgg tcagttcatt tgaacaacct
     3061 gtagtatctg ctctaaccca agtaaattgg ttattaaaaa ttacatttaa tgctgcaatc
     3121 gcttgagcga ctgtaatagt tctttttatt tggcgttctt ttaaattatt tacaacacca
     3181 ctaagtaatt gaatgtcttc tcctcgagat aagtcaagac cattgggagt agttaaatta
     3241 aaatcccctt catagataca ataagaaccg taataatgag tctttcccca ccaatgagct
     3301 acttctataa aggcttccca aaaataataa cctttgccaa gccaagagtt attagcgttt
     3361 gagcaaaaaa caggaacgct agtgttatac ttacgtaatg aagatgcttg gtaaatttta
     3421 gtcatcttca aataacttgt tttttaaaat ggttcctaag ctggataaat taaaagtggg
     3481 taacagtata tgttgacttt gagattgagt gcttaacaag ctgataaagg ctctaatata
     3541 ggggtataaa atggcaatac tgttttgaaa gaaataatca ggaatggatt cttttgtgat
     3601 atcttcatta aattgaaaat tacctatcat gctaatagaa caaaaagctt cttcaaattt
     3661 tttatttcga atag
//
LOCUS       DAOOBB010000188         3618 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_28690_length_3618_cov_3.222846, whole genome shotgun sequence.
ACCESSION   DAOOBB010000188 DAOOBB010000000
VERSION     DAOOBB010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28690_length_3618_cov_3.222846"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="PLT31_08015"
     CDS             complement(<1..856)
                     /locus_tag="PLT31_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95111.1"
                     /translation="MNFKSLIFLSCALLLAACGSESVTNINDEAKETGDISFLILNRS
                     GETLDSAKIYRISDGKTNFTDKFGNAIWKKNNIGTYVFTITKEGYAPMRISVNVQEQG
                     KGNVARVPDVTQEVVMHELGATVQGTLMYRDYSTGNLIAAEGVPVILSFENANFTFSE
                     NAVETDENGEYLFENLPEGVSVTINVPQTTIKKHLYALNVDLDTDIDRANEVLNLGIS
                     QLSLVAKKANLIKTNFTEIDTTTDLSISFSTDLDEDSIPGHWVVTNSYDDIVLTSASL
                     KDSTTVTIE"
     gene            complement(885..3053)
                     /locus_tag="PLT31_08020"
     CDS             complement(885..3053)
                     /locus_tag="PLT31_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HPW95112.1"
                     /translation="MLKKVISCTACATLFFACSDGSIINVNDEAKDKANISFLVLDSY
                     SGEAIDSVEIYRSKDSKTNLTDSLGYSVWKKNAIGNYVFELSKEGYATIRTSVDVVEE
                     GLGDVARVPDVTATVPMYKLGAEVNGTILYTDKETKNKVPAADVTVIITNLNRSIIPN
                     EMSVKTDKNGNYSFSQLPEASSFLITVPQTSIDGNKYEVISSTSVPAQRAGATVDLGV
                     QNLTLVTSDFSLVNSNTNKIDTNTTVTLSFSTILDKDSVIKNIKVLKGNISVLTQVSL
                     STDNKTISIKPFSGSWEDNKNYTISGAVYSSNGSYLNVNKSFTVGGTTTTRKPGQIDE
                     LKVELDGSYLDISWTRKDSLATGYSIYYKNDSMSNYLMYTKLSSGNYTSTSISYSNLL
                     NDDAETTTVNIIVLPYNKAGEADAKRAESVSYTFAAPTAQVSNLSVELNGSYAYIDWD
                     APTTGSYDYRIYYKTPSMDEYVVLTTTISTYYETNYTISNYLESGDESISFKVVPYDD
                     FGESSLSKAVAATLSLAIDVVAGVAAEIDGSYFKVSWNNLANASYYYVYYKTSSMTSY
                     SRTTSTSSLYKNFLISSYVKSGDTSISFKVIASNAANEGVLADAEEVTIEYPSGPEVI
                     TGMTAEVNGELIELSWDAPETGIVKGYYLYYKTSSMTDYLRSSSNPTTTSVNINKALY
                     TETTDTDISFKVTAYNDFGESSIDDTDPVTVDLSTTPDPL"
     gene            3265..3405
                     /locus_tag="PLT31_08025"
     CDS             3265..3405
                     /locus_tag="PLT31_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95113.1"
                     /translation="MNWKNILGVLFIFSISVSAFQTPVSSYGALITEGSNIVSSITKE
                     PV"
BASE COUNT         1034 a          638 c          788 g         1158 t
ORIGIN      
        1 gttcgatggt gaccgttgtc gaatccttta ggctagcact cgtgagaacg atgtcatcat
       61 aagaatttgt aacaacccag tggccaggaa tggaatcttc atcaagatct gtggaaaatg
      121 aaattgagag atcggtagtt gtatcgattt ctgtaaagtt cgtcttaatt aggttagcct
      181 ttttcgcaac caatgaaagc tgggatattc ccaagttcag tacttcgttt gcacgatcaa
      241 tgtccgtatc aagatcgaca ttcagagcat agagatgctt tttgatggtt gtctgaggaa
      301 cgttaattgt aacacttacg ccttcaggaa ggttttcaaa taagtattct ccgttttcat
      361 cagtttctac agcgttttct gagaaggtaa agttggcgtt ttcaaaagaa agaatgacag
      421 gaacaccctc ggcggcaata aggttgcctg tcgaataatc cctgtacatt aatgttccct
      481 gaacagtggc accaagttcg tgcatcacga cttcttgtgt aacatctgga acccgagcaa
      541 cattgccttt tccctgttct tggacgttca cgctaatgcg cattggagca tagccttctt
      601 tggtaatggt aaagacgtat gtaccaatgt tgttcttttt ccaaatggca ttgccgaatt
      661 tatcagtgaa gttagtttta ccgtcagaaa tacggtaaat ttttgctgaa tctagcgttt
      721 caccgcttct gttgagaatt aaaaacgaaa tatcgccagt ttctttggct tcatcgttta
      781 tattggttac agattcactg ccgcaggcgg cgaggaggag tgcgcaagaa aggaatatga
      841 gagatttgaa attcattttc attttaatat tctcctaaat tgtattagag cggatctgga
      901 gtggttgaaa gatcaacggt gacaggatcg gtatcatcaa tagaagattc tccgaagtca
      961 ttgtaagcag ttaccttgaa actaatgtct gtgtcagtag tttctgtgta taaggcttta
     1021 ttaatattta cactagtcgt cgttgggtta gaagatgatc ggaggtaatc agtcatcgaa
     1081 cttgtcttgt aatagagata gtagcccttc acgatccctg tttctggggc atcccagcta
     1141 agttcaatta attcgccgtt aacttcagca gtcatacctg taatgacttc tgggccagat
     1201 ggatattcaa tcgttacttc ttctgcatcg gcgagaaccc cttcattagc agcgtttgac
     1261 gctataacct tgaaggaaat ggatgtatct ccgcttttta cgtaactaga aataaggaag
     1321 tttttgtaca aggaagaagt ggatgtagtc cgagaataag aagtcatcga gcttgttttg
     1381 taataaacat agtagtaact agcatttgca agattattcc agctaacctt aaaatacgat
     1441 ccatcgattt cggcggctac acctgcaact acatctatgg cgagtgagag tgttgctgct
     1501 acggcttttg aaagagagga ttcgccaaag tcatcatagg gcactacctt aaaggaaatc
     1561 gattcatcac ccgattctag atagttggaa atggtgtaat tggtttcgta gtatgtggat
     1621 atggtcgtcg ttagtacgac atattcatcc atagatggtg tcttgtagta gattctgtag
     1681 tcgtagcttc ccgttgttgg agcatcccaa tcaatgtatg cataggagcc attaagttct
     1741 acactcaagt tggaaacttg ggcggtagga gctgcaaacg tgtaagatac cgattcggct
     1801 ctctttgcat ctgcttcacc agctttgttg tacgggagaa caataatgtt gaccgtggta
     1861 gtttcagcat catcgttcag gagatttgag tagctaatgg atgtactcgt atagttaccg
     1921 cttgataatt tagtgtacat caagtaatta ctcatgctat cgtttttgta ataaatacta
     1981 taaccagtgg cgagcgaatc tttgcgagtc caagaaatat ctaggtaact gccatcaagt
     2041 tcaactttga gttcatcaat ttgaccaggt ttcctggtgg tagtggttcc gccaacagtg
     2101 aagcttttgt tcacgttgag atagctacca ttcgaggaat aaacggcacc agaaatggtg
     2161 taattcttgt tgtcttccca agaacccgaa aacggcttga tgctaatggt tttattgtct
     2221 gtcgaaaggc tcacttgggt aagaacgcta atgttgcctt taagaacctt gatgttctta
     2281 atgacagagt ccttgtctag aatggttgag aagctaagag tgacggtggt atttgtgtca
     2341 atcttattcg tattggaatt taccaaagaa aaatcgcttg taactagggt gaggttttga
     2401 actccaagat caacggtcgc accagcacgt tgtgcgggaa cagaagtgct gctgatgacc
     2461 tcatatttat ttccatcgat ggaggtttgt ggaaccgtga ttagaaaact agatgcttcg
     2521 ggtaattgac tgaagctgta gttaccgttt ttgtctgttt ttacggacat ttcgttcggt
     2581 ataatcgaac gattgaggtt ggtaatgata acggtgacgt ctgctgctgg tactttgttt
     2641 ttggtttcct tgtctgtgta aagaatggta ccattgactt cggcacctaa tttatacatg
     2701 ggaacagtgg ctgttacatc gggtacacgc gctacatcgc ctaagccttc ttcaacgacg
     2761 tcaactgatg tacgtatggt ggcatatcct tctttagaaa gttcgaaaac gtaattacca
     2821 attgcatttt ttttccatac agaataaccg agagagtcgg tcaggttcgt tttactgtcc
     2881 ttggaacggt aaatttctac ggaatcgatg gcttcacctg aataagagtc aagaacgaga
     2941 aaagaaatat tggccttgtc tttggcttca tcgtttacgt ttataataga accgtcactg
     3001 catgcaaaaa atagtgttgc gcatgctgta caggaaataa ctttttttag catttatcct
     3061 cctaaaagta ttacacattg tcataatata gctaaggaaa tctttttttt taaaaaaaac
     3121 ttaatttgta ggagcttttt tttagttaaa ttcttgagta tgaaacttga catcaaaata
     3181 catttgacaa taaagtaagt atattataga cattagtaat aaaattaaga ttattcttgg
     3241 tttttaatta aagaaggagc gggtatgaat tggaagaata ttttaggagt attgtttatt
     3301 ttttcaatat ctgtttcagc ttttcaaaca ccagtctctt cttatggagc tctcattaca
     3361 gagggcagta atatagtaag tagcataact aaagagccag tttaacttcg aggagttagt
     3421 ttaggttctg ccaatacatt aattcgaagt aacgtatttt ataatgtaaa tacaataagc
     3481 aacatggtag atccatggaa tgcagaaatg attagacttc cattggaaat cacagacgag
     3541 gaagaatata tagaagattc aacttattac aaaactcttt tcgactctat gattcaggct
     3601 tgtgttgcca aaggaatt
//
LOCUS       DAOOBB010000189         3593 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_28939_length_3593_cov_4.471735, whole genome shotgun sequence.
ACCESSION   DAOOBB010000189 DAOOBB010000000
VERSION     DAOOBB010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28939_length_3593_cov_4.471735"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /locus_tag="PLT31_08030"
     CDS             complement(<1..1094)
                     /locus_tag="PLT31_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPW95114.1"
                     /translation="MSEERKTLGLSNANYLEKLYDGWFLDYASYVILDRAVPYFEDGL
                     KPVQRRILHTLFDMHDGRYHKVANIVGRTMQLHPHGDASIGDALVTMGQKDLLIDPQG
                     NWGNPYTGDSAAAPRYIEGRLTPFALEVLFNPETTNWIPSYDGRNNEPVTLPAKFPLL
                     LAQGVEGIAVGLSTTILPHNFCELCEASIAHLRGKKFELYPDFFTGGIADMSDYNDGL
                     RGGRIKVRAKIEKFDSKTLVIREIPYGTTTGSLIDSIISANDKGKIKIKHVDDNTSQE
                     VEIMIHLHPGTDPQVVIDALYAFTDCEKSLAPNACIIVNKKPQFIGVSEILKLNTDHT
                     VRLLKWELENKLAALSQKWHMTSLEKIFIE"
     gene            complement(1117..2958)
                     /locus_tag="PLT31_08035"
     CDS             complement(1117..2958)
                     /locus_tag="PLT31_08035"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HPW95115.1"
                     /translation="MAAKYNEDSIKSLDWHEHIRLRPGMYIGKLGDGQSPDDGIYVLA
                     KEIIDNSIDEYVMGAGKKIEIEIEDRSVRIRDYGRGIPLGKVVDCVSKINTGGKYDSE
                     AFQKSVGLNGVGTKAVNALSESFIVQSFRDGRCKKAEFCRGILVKEYKESATSEKNGT
                     EIIFKPDPEIFKNYRYLPAYMDEKIWNYAYLNTGLSLIMNGKTYSSTNGLLDLLQKRV
                     DENIRYDVIHFKDKDIEVAFTHGNQYGEHYYSFVNGQHTTQGGTHQAAFREGVVKGAR
                     DHFKKDLDPSDVRNCIIGAISIRIQEPVFESQTKTKLGSTSTAPNGPALRSWIVDYVA
                     KEFDNYLHKNPDTEKKLLDRITQNERERKEIAGIKKLANERAKKANLHNKKLRDCKVH
                     LTEVKSPLHQETMIFITEGDSASGSITKARNVQTQAVFSLRGKPLNSFGQTKKVVYEN
                     EEFNLLQHALDIENGLENLRYNKVIIATDADVDGMHIRLLLMTFFLQFFPELVEQKHL
                     YILQTPLFRVRNKKETIYCYDEDERDKASTSLGKDGLEITRFKGLGEISPSEFGQFIG
                     EDMRLESVMMPPDASLGKMLEYYMGKNSPTRQDHIVTNLRAEAVEEL"
BASE COUNT         1056 a          693 c          691 g         1153 t
ORIGIN      
        1 ttttctataa agattttttc gagactcgtc atgtgccatt tttggctaag tgcagcgagc
       61 ttgttttcta actcccactt tagaagacgg actgtatgat cggtattcag ttttagaatt
      121 tctgaaaccc caataaattg tggcttttta ttcacaataa tacaggcgtt aggagctaga
      181 gatttttcac aatcagtaaa ggcataaaga gcatcaatca ccacttgcgg gtctgtgcct
      241 gggtgtagat ggatcatgat ttctacttct tgggaagtat tatcatctac gtgtttaatc
      301 ttaattttgc ctttatcatt tgcactaatg atgctatcaa ttaaagaacc tgtggtggtt
      361 ccataaggaa tttcacgaat gacgagcgtt ttggaatcga acttttcgat tttggcgcgt
      421 actttaattc gaccgcctcg taagccatcg ttataatcgc tcatgtcggc gatgccgcca
      481 gtaaagaagt cggggtataa ctcgaacttt ttaccgcgaa gatgagcgat ggaagcttca
      541 cagagttcgc agaaattatg aggcaaaatg gttgtagata aacctacggc aataccttca
      601 acaccttgag cgagtaaaag aggaaacttt gcaggaagcg tgacgggctc attatttcgg
      661 ccgtcataac ttggtatcca gttggtggtt tctgggttaa ataacacttc aagagcaaat
      721 ggagtgagcc taccttcaat ataacgaggt gcggcagcag agtcgcccgt ataagggttt
      781 ccccagtttc cttgaggatc aattaacaag tctttttgcc ccatggttac taatgcatca
      841 ccaatagagg catctccatg aggatgcaat tgcatggtgc ggcctacaat gtttgcgact
      901 ttgtgataac gaccgtcgtg catatcaaat agggtgtgca aaatacgacg ttgtacaggc
      961 tttaaaccat cttcaaaata aggaacagca cggtcaagaa tcacatagct tgcataatcc
     1021 aagaaccagc cgtcataaag cttttctaaa tagttggcgt ttgataatcc gagggttttt
     1081 ctttcttcac tcataaatat tctcttcttc tattttttag agctcttcta cagcttctgc
     1141 acggaggttc gtgacaatgt gatcttgcct tgtaggagag tttttaccca tataatactc
     1201 aagcatttta cctaaagatg catcaggagg catcatcaca gattcaagcc tcatgtcttc
     1261 accaataaat tgcccaaatt cagaaggact aatttcaccg agacctttga atcgtgtgat
     1321 ttcaagacca tcttttccta gagaagtact ggctttatcg cgttcgtctt catcgtagca
     1381 atagatggtt tcttttttat ttcgcactct aaaaagaggc gtttgtaaaa tataaaggtg
     1441 tttttgttct acaagttcag ggaaaaactg caagaagaaa gtcattaata ataaccgaat
     1501 atgcatacca tcgacatcgg catcagtagc gataatgact ttattgtagc gcagattttc
     1561 aagcccgttt tcaatatcta gggcatgctg caaaaggttg aattcttcgt tttcatagac
     1621 gactttcttc gtttgcccaa aactgttgag aggcttacct cttaagctga aaaccgcttg
     1681 cgtttgaacg tttcttgctt tggttattga accagaagca gagtcccctt cggtaataaa
     1741 aatcatggtc tcttggtgaa gagggctttt tacctcggta agatgaacct tacaatcgcg
     1801 aagcttttta ttatgaagat tcgctttttt agcacgctca ttggcgagct ttttaatgcc
     1861 agcgatttct ttacgctcgc gttcattttg cgtaatacga tctagaagct tcttttcggt
     1921 gtctgggttt ttatgaagat aattgtcaaa ttctttagcg acataatcca caatccagct
     1981 acgtagagca gggccattgg gagctgtaga agtactgccg agcttcgttt tcgtttgaga
     2041 ttcgaaaaca ggctcttgaa ttcggataga aatcgcgcca ataatacagt ttcttacatc
     2101 cgaaggatca agatcttttt tgaaatggtc tctagcgcct ttgacaacgc cttcacggaa
     2161 agcggcttgg tgagtaccac cttgagttgt atgttgaccg ttgacgaagc tataatagtg
     2221 ttcgccatat tgattgccat gtgtaaaagc cacttcgatg tctttatctt tgaaatgaat
     2281 cacatcgtag cgaatgtttt catccactct tttttgaagt aaatctaaaa gaccgttggt
     2341 actggagtag gtcttcccgt tcatgatgag agaaaggcct gtatttaaat aagcgtagtt
     2401 ccagatcttt tcgtccatgt aggcgggtaa ataacgataa ttcttaaaaa tttctgggtc
     2461 aggcttaaaa atgatctctg taccattttt ttctgaagtc gctgattctt tgtattcttt
     2521 aacaagaata ccacgacaga attctgcttt tttgcaacga ccatctctaa aactttgcac
     2581 aataaaagac tcagaaagtg cgtttacggc tttagtacca acaccattca aaccaacgga
     2641 cttctggaag gcttcagaat cgtatttacc accagtattg attttactaa cgcaatcaac
     2701 cacttttccg agtggaatcc cacgcccata gtctcgaata cgcacagatc gatcttcgat
     2761 ttcaatttca atctttttac cagcacccat gacgtattca tcaatggagt tgtctataat
     2821 ttctttggct aagacgtaaa taccatcatc aggactttga ccatcaccta gcttaccaat
     2881 atacatgcct ggacggagtc taatatgttc atgccaatcc agagatttaa tgctgtcttc
     2941 gttatatttt gctgccataa gtattcaata attttagaat ttgtccaaac atagtcaaaa
     3001 atcatctaaa gcaaccatca aataaggctt aaacttgcgg ttttggccag tttctaaaaa
     3061 cgaattatta atgattgcct aaaatgaaaa agcatgtttc attaaaaatc aaatatagaa
     3121 aaaaatattc tactgcacaa atgtacaaaa agaaaaaggg gaagaaaagc ctttttttgt
     3181 tgcttttatc tacctgctct cgttgataca accagcaaat caaagaagac cgcttgctac
     3241 agctacacct gcaaatcttt tgaaaatatt catttccgct ccgtttgatc agtttattca
     3301 caagaaaagt aaatcaaaga gagcaaaaaa ggcgattgta acaaaaacaa ctaaaaaagg
     3361 atctttcaag caaggtaggc tgttttaggt ttgttagtta gaaaaaagag aataacactg
     3421 ttttatttat ttagtataaa taatgttttt tcttcgtctt gttataatga cattatttgt
     3481 cattactatt tagttatttt tttataaagg acttttttaa gtggtgaatt atggctggtc
     3541 taattaaaga tatttatgaa gtttttgttg gacaaggata ttcctcttgt aag
//
LOCUS       DAOOBB010000190         3586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_28996_length_3586_cov_3.508638, whole genome shotgun sequence.
ACCESSION   DAOOBB010000190 DAOOBB010000000
VERSION     DAOOBB010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28996_length_3586_cov_3.508638"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="PLT31_08040"
     CDS             complement(<1..919)
                     /locus_tag="PLT31_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95116.1"
                     /translation="MNFHFLKFLKIAALISVASFTFVGCGDDEPTKPGNGESSSSVAP
                     PMSSPEATKISPIKINGFGVEQISAERFNVTGNVVIDYTDTTVKNVDSVRIDNIQFNV
                     GLVQGNALGNVGATVEMIAPPTFPTEQVVNFGEAGAKIDISTFEACGDFVVEVIVYSS
                     TPDGKNKYISKESATFSKPAATYCPEPVSSSSVAEILIEMDSYVVTLTTSRTIGDVAI
                     DFETGSVFPASALALNKAVIDAVLVWEGGVGVLKSARIAGLDNYDALAGGNDTKFAEE
                     MKAPSGEEMPAPVYTNAFSYSTPTITALEE"
     gene            complement(1107..3221)
                     /gene="uvrB"
                     /locus_tag="PLT31_08045"
     CDS             complement(1107..3221)
                     /gene="uvrB"
                     /locus_tag="PLT31_08045"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HPW95117.1"
                     /translation="MARARKTISPNPFQKQILKKLSPEESRPGELKEFMPSSRAEFDL
                     VSSYSPAGDQPLAIDEITRAFSADERFQCLLGVTGSGKTFTMANVIKNVGKPTLILTH
                     NKTLAAQLYQEFKAFFPNNAVEYFVSYYDYYQPEAYIVHSDTFIEKDASINDEIDKLR
                     LRATANLLTRRDVIIVASVSCIYGLGSPGEYFDFMIRLQVGDVIERDEILKKLVHIQY
                     ERNDMALDRGRFRVRGDVIEIYPSYDDIGIRIELWGDTIDRLSRFHLVTGEVLTELQE
                     INVAPAKHFVTREDSRLSIVQNIEKELTERLEVLEREGKVLEAARLASRTRYDMEMIR
                     ETGVCSGIENYSRIIEERIPGSRPFTLIDYFGKDWLMMIDESHVSIPQVGGMFEGDKS
                     RKTTLVEYGFRLPCAIDNRPMSFDEFEYMYPPSVLFVSATPGDYELRKTQGAVVEQIN
                     RPTGLLDPQIEVFPVKGQMDVLIDRMTQVIQKDERVLVTTLTKKMAQDLTDYLKEMNI
                     RARYLHSEIKTLERAQIIRDLRQGEFDVLVGINLLREGLDLPEVSLVAILDADKEGFL
                     RNFRSLIQTMGRASRHLNGTVLLFADVMTDSLKKAIAETKRRRQTQEAFNTLHGIIPK
                     SVTRRLENQLEIEDPLTSLFGDEKLKDGVSSDLEATPESLASIEELEVQMKAAAALLD
                     FEEAARIRDIIRERSSSEERKK"
     gene            complement(3321..>3586)
                     /locus_tag="PLT31_08050"
     CDS             complement(3321..>3586)
                     /locus_tag="PLT31_08050"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPW95118.1"
                     /translation="AEIGYWLSKPFQRKGLVTDSVKGLIRLAFEKMGLHRIQIKCAVN
                     NIQSKNIPRRLNFIFEGTERDGELLADGTYTDVEVYSFLNKKR"
BASE COUNT         1091 a          784 c          570 g         1141 t
ORIGIN      
        1 gttcttctaa agcagttatt gttggagtac tataggagaa ggcattagta taaactggag
       61 caggcatttc ttcaccagaa ggagctttca tttcttcagc aaatttggta tcattaccac
      121 ctgctaatgc gtcataatta tctagaccag caatacgagc actctttaag actcctacac
      181 caccttccca tacaagaaca gcgtcaatga cagctttatt tagtgctaat gcagatgcag
      241 ggaaaacact tcctgtttca aaatctattg ctacatcgcc tattgttcta cttgtagtta
      301 aagtgacaac ataagaatcc atctctataa gtatttccgc aactgaactg cttgatactg
      361 gttctgggca gtatgtagca gctggtttag aaaaagtggc tgattctttt gaaatgtact
      421 tatttttacc atctggagtg cttgaatata caatcacctc tacaacaaaa tcaccacagg
      481 cttcaaatgt agagatatca attttagctc cagcttcacc aaagtttaca acttgttccg
      541 tagggaaagt tggaggagca atcatttcaa cagtagcacc tacatttcct aaagcgtttc
      601 cttgaacaag gcctacatta aattgaatat tatctattct cacagaatcg acattcttca
      661 cagtcgtatc tgtatagtca atgactacat tgcccgtcac gttaaagcgc tctgcactta
      721 tctgctccac accaaaacca ttgattttaa ttggagaaat tttcgttgct tctggggatg
      781 acataggtgg agccacagaa gaagaagatt ctccgtttcc aggtttagtc ggttcatcat
      841 caccacatcc aacaaaagtg aacgaagcta cactgataag cgcagcgatt tttaagaatt
      901 tcaaaaaatg gaagttcata ataacctctt aaatttgttt tcttcaaaat aaacaaaaaa
      961 tcaaaaaaaa gctatgcatt tttacttaac acctcattaa aagaggtttt ctgttatgaa
     1021 aatagacaca aaagaagcat tgatgctaac aaatcgataa agaactattt atttatgagc
     1081 ttaatcaatc cattttgacc aaaatattac ttttttcttt cttctgaaga agatctttcc
     1141 ctaatgatat cccgtattct ggcagcttct tcaaaatcaa gcaatgcggc agcggccttc
     1201 atttgaactt ctaattcttc aatagaagcc aaagactctg gtgttgcctc taaatcacta
     1261 gaaacgccat cttttaactt ttcatcccca aataatgaag ttaaaggatc ctctatttct
     1321 agttggtttt caaggcgacg agtcactgat tttggaataa taccatgtaa agtattaaaa
     1381 gcttcttgtg tttggcgacg tcgttttgtt tctgcaatcg ccttttttaa gctatctgtc
     1441 atgacatcag caaatagaag tacggttcca tttaagtgac gactagctct tcccatcgtt
     1501 tgaatcaggc ttctaaaatt acgtaaaaag ccctctttat ctgcatctaa aatggctact
     1561 aaagatactt ctggtaaatc taacccttct cgaagtaaat tgatgcccac taagacatca
     1621 aattcgccct gacgtaaatc tctaattatt tgagctcgtt ccaaggtttt gatttcactg
     1681 tgcaaatacc tagctcttat attcatttcc tttaagtaat ctgttaaatc ttgagccatt
     1741 tttttagtta aggtagtgac taaaactcgt tcatcttttt gaatcacttg tgtcattcga
     1801 tcgattaaca catccatctg ccctttcaca gggaatactt caatttgagg gtctaataat
     1861 cctgttggcc tattgatttg ttccacaacg gcgccctgcg tttttcttaa ttcataatcc
     1921 ccaggagttg cactcacaaa aagcacggat ggcgggtaca tgtattcaaa ttcatcaaag
     1981 ctcatgggcc tattatcaat agcacaagga agcctaaacc catattctac tagcgttgtt
     2041 tttctcgatt tgtctccttc aaacattcct cccacttgag gaatactcac atgagattca
     2101 tcaatcatca ttagccaatc tttgccaaaa taatcaatca aggtaaaagg acgagaacca
     2161 ggaatccgct cttcaatgat tcgagaataa ttttcaattc cagaacaaac acctgtttca
     2221 cggatcatct ccatatcata tcgagtgcga ctggcaagcc ttgccgcttc taacaccttc
     2281 ccttctcgct ccagaacctc tagcctttct gtaagttctt tttcaatatt ttgaacgata
     2341 cttaaacggc tgtcctctct agtgacaaaa tgcttcgctg gggccacatt aatttcttga
     2401 agttcagtaa gcacttctcc tgtaactagg tgaaaacggc ttaatcgatc aatggtatct
     2461 ccccaaagtt caatccgaat cccaatatca tcataactgg ggtaaatttc aatcacatcc
     2521 ccacgcacgc gaaaccgccc gcgatctagc gccatgtcat ttcgttcata ctgaatatga
     2581 actaactttt ttaatatctc atctcgttca atcacatctc ctacttgcag acggatcata
     2641 aaatcaaaat attctccagg gctccctaac ccataaatac agctcacaga agcaacaata
     2701 attacgtctc ggcgagtgag taaattggct gtagctctta atcgaagctt atcaatttca
     2761 tcattaatac tggcatcttt ttcaataaaa gtatctgaat ggacgatgta tgcctctggc
     2821 tgataataat cgtaataact cacaaaatac tcaaccgcat tattagggaa aaaggcctta
     2881 aattcttgat acaactgagc ggctagcgtt ttattatgtg tcaatattaa tgtgggtttt
     2941 cccacatttt ttatcacatt agccatggta aaggtttttc ctgatccagt gaccccaagc
     3001 aaacattgga agcgctcgtc tgctgaaaaa gccctcgtga tttcatcaat agctaagggt
     3061 tggtctcctg cgggagagta tgaactcact aaatcaaatt cagccctcga agaaggcata
     3121 aattctttta attctccagg acgactttct tctggagaaa gcttttttaa aatctgtttt
     3181 tgaaaagggt tcggactaat tgtttttctt gctctagcca taaagattca atttagaaac
     3241 tcaactctca ttagtgtatt tcttgattaa caaatgagcc ctttacccta taaaaaggcc
     3301 tttttaacga ttcctatttt ttaacgtttt ttatttagaa aactatatac ttctacatca
     3361 gtataagtac cgtcagctaa aagttcacca tctcgctcag tcccttcaaa aataaaattg
     3421 agacgtcttg gaatgttttt actttgaata ttatttaccg cacattttat ctgaatgcga
     3481 tgaagaccca ttttttcaaa agcaagccga attaaaccct ttaccgaatc agtaactagc
     3541 ccttttcttt gaaaaggttt ggacaaccaa taacctattt cggccc
//
LOCUS       DAOOBB010000191         3543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_29441_length_3543_cov_4.526376, whole genome shotgun sequence.
ACCESSION   DAOOBB010000191 DAOOBB010000000
VERSION     DAOOBB010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29441_length_3543_cov_4.526376"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="PLT31_08055"
     CDS             complement(<1..625)
                     /locus_tag="PLT31_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95119.1"
                     /translation="MSLNLNEYEIPQNNHNKKRQGFPLIRILLLVAVLFYLFQGNYFH
                     KLYDYFTSSAHLNENVNPEPLSWDQECSSYRGKFFLVRDSVGQCSWKINQNDSLLFNH
                     DLWRYLIEMELKNSLIIHWVAPIKNFRSPWFISLKTDSLEQNFFRVPFKDSTFIWVEH
                     QSLCRFPGFCPENPLEGGALPIPEDFDFQGRELLLMKDQFMGIGESPV"
     gene            complement(628..1269)
                     /locus_tag="PLT31_08060"
     CDS             complement(628..1269)
                     /locus_tag="PLT31_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95120.1"
                     /translation="MVQLKNYREVGIFDLLSAPLNPIGAFDAESFKVDVRALINKKSN
                     FIAVDLTGIDFLYSDAYNALMLFQKELATTGGVIGILTNNKIIIDGLHRAGLDKKVKL
                     FTTEAALIDFSTHSTAIKDDVEIPLDESIRGAVSMNQVNSNNVSSEEKKPHKRATGRF
                     TKSFNAITKEDSSKVTSGMQTPFLDDTSSSHLLLWIMIILFVAGALIAYFVLK"
     gene            complement(1329..2486)
                     /locus_tag="PLT31_08065"
     CDS             complement(1329..2486)
                     /locus_tag="PLT31_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95121.1"
                     /translation="MKFYVPTFCLKIGFIALLSMASNAVSLDRVSGANATLDINAGAR
                     SAALGGTTLAIDNDLSVLMSNPGVLSSLLHSWVGFSHVSYYEGTQYDFAAMSLPLGED
                     HGVGIAFSRFGANDIPWIKEGDPIPEGSDYNTLNIADYTLTLAWGKRFGRFDVGVSFH
                     GLYRELDQTGYGFRGDASLRYFILKPLSVSALLKGWTSSAATWESGTFEYSSPELYLA
                     TKFDQKVKYLYGDFSFYWQSAGIFHREARDLDWVDSPSGGRLWESPIDWFLGSRAGLE
                     FAFDFGLSLRFGLASLTTVQSMTAGAGLELARFLRVDYAFESHPTLSAVHRVSLSFSP
                     WLFSHPPKEKRSKMVFESENQVKEKENLRPLAEEPPPSEEPEEDLGTHWVE"
     gene            2537..3433
                     /locus_tag="PLT31_08070"
     CDS             2537..3433
                     /locus_tag="PLT31_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPW95122.1"
                     /translation="MSELLQDIENFIDYITHQRLYSPRTIETYQKTLRKFLKGLPENP
                     TRNDFQIASIRQFIWVLKTEEKLAPTSIGLHLACLKSFGKYMVRSHIITTNPAARIPM
                     PKKPKRLVSFLSQKDLNVEKFAESDTLSLPLLRARLLLEFIYGSGLRISECVSLTWES
                     IDRTAEQVRVVGKGNKERIIPITHELQKCLTQYQFLLEQEKHPITIRGPVFVNKTGAA
                     YNVRTLRLDIEHLLRSIGWEGKASPHVLRHSFATHLLENGADLLSVKEMLGHNSLSTT
                     QVYTHVSAERLKKSFKKAHPRA"
BASE COUNT         1231 a          719 c          627 g          966 t
ORIGIN      
        1 agacaggaga ttctccaatt cccataaatt ggtctttcat tagcaataat tctcgtccct
       61 gaaaatcaaa gtcctcagga attggaagcg cgccaccttc taaaggattt tcggggcaaa
      121 agccagggaa ccgacataga gattgatgct ctacccaaat aaaagtggaa tctttaaacg
      181 gcactctaaa gaaattttgc tctaaagaat ccgtctttaa agaaataaac caaggcgaac
      241 gaaaattttt aatgggagcg acccaatgaa taatcaaaga attctttaac tccatttcaa
      301 ttaaatagcg ccaaagatca tgattaaaca ataaagaatc attttgattt atcttccaag
      361 agcactgccc caccgaatca cgcactaaaa aaaacttgcc gcgataagag gaacattctt
      421 gatcccaaga tagtggttct ggattgacgt tttcgtttaa gtgagcagag gaagtaaagt
      481 aatcataaag cttatgaaaa taattcccct gaaataaata aaatagaact gcaactaata
      541 ataatattcg aattaaaggg aatccctgac gctttttatt atgattattc tgagggattt
      601 catattcgtt aagatttaaa gacatatcta tttaaggaca aagtaagcga ttaatgctcc
      661 tgccacaaaa agaataatca tgatccataa aagtaagtgt gaagaagagg tgtcatctaa
      721 aaatggtgtt tgcataccag aagttacttt tgatgaatct tcttttgtaa ttgcattaaa
      781 gcttttagta aagcggcctg ttgcccgttt atgaggtttc ttttcttctg aagacacatt
      841 attactattg acttgattca ttgaaacagc accgcgtata ctttcatcta aaggaatttc
      901 tacgtcatct ttaattgctg tagaatgagt agaaaaatca attaaagccg cttctgttgt
      961 aaataatttg acttttttat ctaaacctgc acgatgcaac ccgtcaataa tgattttatt
     1021 attcgttaag atgccaatca ctccgccagt ggtagctagt tctttttgaa acaacattaa
     1081 ggcattataa gcgtcactat acaaaaagtc tatcccagtg agatctactg caataaaatt
     1141 tgatttttta ttaattaaag cacggacatc taccttgaaa ctttccgcat caaacgcacc
     1201 aatgggattc aaaggagctg acaataaatc aaaaatccca acttcacgat aattctttaa
     1261 ttgcaccata aacaaaaata tagcctaaaa taaatgaact gtattcaaaa tcacataaaa
     1321 aaaacatttt actccaccca atgtgttccc agatcctctt caggttcctc tgaaggaggt
     1381 ggttcttcag ctaaaggcct taaattttct ttttctttta cttgattctc tgattcaaaa
     1441 accattttag aacgtttttc tttaggcgga tggctaaata gccaaggact gaagctcaaa
     1501 ctgacgcgat gcacagcaga aagggtcggg tgagattcaa aagcatagtc cactctaaga
     1561 aaacgagcaa gttcaagccc tgctccagca gtcatactct gcacggtagt taaactagca
     1621 agtccaaaac gaagagaaag cccaaaatca aatgcaaatt ctaggccagc tcggctccct
     1681 aaaaaccagt ctataggaga ttcccagaga cggcctccag aaggagaatc aacccagtct
     1741 aaatctcttg cctcacggtg gaatatgcca gcactttgcc aataaaaaga aaagtcgcca
     1801 tagaggtatt tcactttttg gtcgaatttt gtggctaaat acaattctgg agaagaatat
     1861 tcaaaagtac cagactccca agtagctgca gaagaagtcc accctttaag taaagctgaa
     1921 acagaaagag gttttaatat aaaataccgc aaagaagcat caccacgaaa accataaccc
     1981 gtttggtcta attcgcgata aagcccatga aacgaaacgc ccacatcaaa acggccgaaa
     2041 cgcttacccc aagctaaagt gagagtgtag tcggcaatgt ttaaggtatt gtaatcagag
     2101 ccttctggaa taggatcgcc ctctttaatc caagggatat catttgcacc aaaacgagaa
     2161 aaggcaatgc caacgccgtg atcttcacct aaaggcaaag acatggctgc aaaatcatat
     2221 tgcgtgcctt cataataaga aacgtgagaa aaaccaaccc aactatgaag aagcgaagaa
     2281 agcacccctg gattagacat taagacgctt aaatcattat caatagcaag agtagtacca
     2341 ccaagtgccg cagaccttgc cccagcgtta atatcaagcg ttgcattagc accagaaact
     2401 cgatctaatg atacggcatt agaagccatg cttaaaagag caatgaatcc gatttttaaa
     2461 caaaatgtag gtacataaaa cttcataaag atctatactt aatcagagta gatgacaaaa
     2521 cataagatac aaatttgtga gtgaactatt acaagatata gagaatttta ttgattatat
     2581 aactcatcag cggttatatt ctccaagaac tattgaaacg tatcaaaaaa cactccgtaa
     2641 attcttaaaa ggcttacctg aaaaccctac aagaaatgat tttcaaattg cctctattcg
     2701 gcagtttatt tgggttctaa aaacagaaga aaaactagcc cccactagca ttggattaca
     2761 cctagcgtgc ctaaaaagtt ttggaaaata tatggtgaga tcccatataa ttacaacaaa
     2821 cccagcagcc agaatcccca tgcctaaaaa gcctaagagg ctcgtttctt ttttatctca
     2881 aaaagattta aacgtggaaa aatttgcaga aagcgatacg ctttcacttc ctcttttacg
     2941 tgcccgattg ttgctcgaat ttatttatgg atctggactt cgtatttcag aatgcgtttc
     3001 tctcacttgg gaaagcattg accgcacggc agaacaagta agagtggtag gtaaagggaa
     3061 caaagagcgc attatcccta tcactcatga actccaaaaa tgcttaacac aatatcaatt
     3121 tcttttggag caagagaagc acccaattac tattcgtggt cctgtttttg tgaataaaac
     3181 aggagccgct tataatgttc gaacactacg attggatatt gaacatttgc ttcgctcgat
     3241 tggttgggaa ggcaaagcga gccctcacgt attaaggcac agctttgcca cacacttatt
     3301 agaaaacgga gcggatttat tgagcgttaa agaaatgctt ggtcacaata gcttatcaac
     3361 aacacaagtc tatactcacg taagtgccga acgcttaaaa aagagcttta agaaagctca
     3421 ccctcgtgca tgatttttaa ttcgatttaa aaatgctcgc tcgtctatgc cgtagcacgc
     3481 acgactctgg cggaaaactt ttatttttgg cttttttgcc atccgcttaa ttgttttgat
     3541 aca
//
LOCUS       DAOOBB010000192         3532 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_29555_length_3532_cov_4.657751, whole genome shotgun sequence.
ACCESSION   DAOOBB010000192 DAOOBB010000000
VERSION     DAOOBB010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29555_length_3532_cov_4.657751"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="PLT31_08075"
     CDS             <1..739
                     /locus_tag="PLT31_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HPW95123.1"
                     /translation="VHDVRVIRCPRHAASCPVSIGVSCSADRNIRAKIDADGLWIEKM
                     EHNPEQYLPKAGEETPLAPAVEVNLDRPMSEVLKQLSQYPVKTRLSLSGTMIVARDIA
                     HAKLAEMLDRGEDLPDYFKNHPVYYAGPAKTPEGMPTGSFGPTTAGRMDSYVASFQEK
                     GGSMIMVAKGNRSKDVTDACKKFGGFYLGSIGGPAAILAEQNILSNDVVAFPELGMEA
                     IRKITIKDFPAFIIVDDKGNDFFDGLL"
     gene            1083..1700
                     /gene="hisH"
                     /locus_tag="PLT31_08080"
     CDS             1083..1700
                     /gene="hisH"
                     /locus_tag="PLT31_08080"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HPW95124.1"
                     /translation="MNPIIVVDYGAGNLTSVFNALSFLNIQAKVSRNPEEIAASEKII
                     FPGVGAAGSAMQVLTDSGIGEAVSTVVKKGRPVLGICIGCQIILDSSDEDGGVPTLGL
                     LHGKAVRFKDEPGLKIPHMGWNQVIYTKEHPIMKGIPDGSDFYYVHSYHPLVANENAF
                     AETTYGSQTFQCIVGKDNLIATQFHLEKSGSIGLQVLKNFSSWNV"
     gene            complement(1702..2778)
                     /locus_tag="PLT31_08085"
     CDS             complement(1702..2778)
                     /locus_tag="PLT31_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04133 family radical SAM/SPASM protein"
                     /protein_id="HPW95125.1"
                     /translation="MNISLKKRLALEFYRLYRHNEIKMHPLTYFFWECTLRCNLNCLH
                     CGSDCVKEAIPDMPREDFMKVLDSLAPHIDPKNFIVVITGGEPLMRKDLEECGKEIKK
                     RGYAWGMVTNGLAMTPSRFTGLLNAGLKSLTISLDGLEHSHNHFRGDKHSFEHAWKTI
                     RMASQVKNFTYDAMTCVNKQNLAELPEIRDMLIKNGIKRWRIATVFPKGRAKENPLFQ
                     LTNSEFKQVFDFIRETKQLKKISVNYGCEGFLGSYEKSVRNYPFFCRAGVNISSVLCD
                     GSISACPSLRGDYIQGNIYKDDLWDVWQNRYQVMRDRRWAKIDECKSCKYWRYCEGSS
                     LHLRDEKTKELAYCHVKRLEDAGA"
     gene            complement(2892..>3532)
                     /locus_tag="PLT31_08090"
     CDS             complement(2892..>3532)
                     /locus_tag="PLT31_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95126.1"
                     /translation="TAFWSSCDSDSTTNPPVECEGQECVQESSSSTETPSSSSIESSS
                     SEDIQMSAMLYGVFYSSSDPSFSSSSCSEIEALYGIFYSSSDAALSSSEDIQYSSSLY
                     GVFYSSSDPSFSSSSEQRKIICYNYNSNGKITCDDGLTCEETVNPISDCTEGPCPVYG
                     VPSPQDISYKCDNDETYTPEEFLKDHEIWSNAALYGVFPAKPDDNNSSDKKN"
BASE COUNT         1021 a          671 c          736 g         1104 t
ORIGIN      
        1 tgtacacgat gttcgtgtga ttcgttgccc tcgtcatgcg gctagttgcc ctgtctctat
       61 tggtgtttct tgctctgcgg accgaaacat tcgagcaaaa attgatgctg atggcttatg
      121 gattgaaaaa atggaacata atccagaaca ataccttcca aaagcgggcg aagagactcc
      181 tcttgctcct gcggtggaag tgaatttaga ccgtccaatg agcgaagtct tgaaacaact
      241 ttcgcagtac cctgtaaaaa ctcgtctttc tctttcgggc acgatgattg tggctcgaga
      301 catcgctcat gctaagttag cggagatgct tgatcgaggc gaagatttac ctgattactt
      361 caaaaaccat cctgtttatt atgctggtcc tgcaaagact ccagaaggca tgcctactgg
      421 aagctttggc ccaactacag ctggtcgtat ggattcttat gtggcttcgt tccaagaaaa
      481 gggcggcagc atgatcatgg tggctaaggg aaatcgttcc aaagatgtta ccgatgcatg
      541 caaaaaattt ggaggcttct acttaggctc cataggcggt cctgcggcga ttcttgcgga
      601 acaaaacatc ttgtctaatg atgtggtggc gttccctgaa cttggaatgg aagcaatccg
      661 aaaaatcacg atcaaggatt tccctgcatt tattattgta gatgataaag gaaatgattt
      721 ctttgatgga ttgttatagt ctgccttaaa agaggcatgc cttacataac ttgattttaa
      781 cataaaatct tttcaaaaaa acagactgtt ctaagtaaca gcctgttttt ttatgctatt
      841 aagaaaaatg atatgaacca caaaggattc ttgtttataa aggacgagat gcgaggtgat
      901 gacaatctat ttttataaca ataaacagcg agacacttca tgatgattca aaaaagagga
      961 taaaaaagaa ttagcttttt atttatcatc aagtgaaatg aaaaaaaata ttgtggtgaa
     1021 ataccgagac agattgaggt attcatgttg gatagaacgg tgttcttttt tatatttaga
     1081 aaatgaatcc tattatagtt gttgattacg gtgctggaaa tttaacatct gtttttaatg
     1141 cactttcttt tttgaatatt caagcaaaag taagccgaaa tccagaggaa attgccgctt
     1201 cagagaaaat tattttccct ggtgtgggtg ctgctggctc agccatgcaa gttttaacgg
     1261 attctggtat tggagaagcg gtctctacgg tagtaaaaaa aggtcgccct gttttaggta
     1321 tttgcattgg gtgtcaaatt attctcgatt ccagtgatga agatggtggc gttcctacgt
     1381 taggcctttt acatggaaaa gcggtacgat ttaaggatga gcctggatta aaaatcccgc
     1441 atatgggttg gaaccaagtt atttacacta aagaacatcc tatcatgaag ggtattccag
     1501 acggttctga tttttattat gtgcattctt atcacccttt agtggcaaat gaaaatgctt
     1561 ttgcagaaac cacttatggc tcgcaaactt ttcaatgtat cgtcggaaaa gacaacttga
     1621 ttgcaactca attccactta gaaaaaagcg gttctattgg gcttcaagta ttaaaaaatt
     1681 tttctagctg gaacgtttag attaagctcc tgcgtcttct agtcttttga cgtgacagta
     1741 ggcgagttct tttgtttttt cgtcgcgtaa atggaggctt gaaccttcgc aataacgcca
     1801 gtatttacat gacttacatt catcaatctt tgcccaacga cggtcacgca tgacttgata
     1861 gcggttttgc caaacatccc aaagatcgtc tttgtagatg ttcccttgga tgtaatcacc
     1921 gcggagactt gggcaagcag aaatactacc atcacaaagt acagagctga tatttacgcc
     1981 tgcacggcaa aagaatgggt aattgcgtac acttttttca tagcttccaa gaaaaccttc
     2041 acaaccatag ttgacgctaa tctttttgag ctgtttggtt tctcgaataa agtcaaagac
     2101 ttgtttgaat tcgctattgg taagctgaaa taggggattc tctttagctc gacctttagg
     2161 gaacaccgtt gcaatgcgcc agcgtttaat gccatttttg ataagcatat cccggatttc
     2221 aggcagttca gctaagttct gtttgtttac gcaggtcatg gcatcgtaag tgaaattttt
     2281 aacttgagaa gccattctta ttgttttcca agcatgctca aagctgtgtt tatcaccacg
     2341 aaaatgatta tggctatgtt ctaaaccatc taaactaata gtcaggctct taagccctgc
     2401 gtttaatagt cctgtaaaac gggatggtgt catggctagg ccattggtga ccattcccca
     2461 agcatatcct ctttttttaa tttctttgcc acattcttca aggtcttttc gcatgagagg
     2521 ctcgccacca gtaatgacca caatgaaatt tttagggtcg atatgaggcg caagagaatc
     2581 aagcactttc ataaaatctt cacgaggcat gtctgggata gcttctttta cgcagtcact
     2641 tccacaatgc aaacaattga gattgcagcg cagggtgcat tcccaaaaaa agtatgtcag
     2701 agggtgcatt tttatttcgt tgtggcgata aagtctataa aattcaagag caaggcgttt
     2761 ttttaagcta atgttcattt aatgaattat cctaaggagt ggtgatcttt ttctatttta
     2821 ttaaccagcc cagaatatgg ccgcgatact gaggacgata tttttccaat gctttcgtag
     2881 cttaatattc attagttctt tttatcacta gaattattat catcaggttt agcggggaaa
     2941 actccatata aggctgcatt gctccaaatt tcgtgatctt ttaagaattc ctctggagtg
     3001 tacgtttcat cgttatcgca tttgtagctt atatcttgag gagatggtac tccatatacg
     3061 gggcaagggc cttcggtaca atcacttata ggatttaccg tttcttcgca ggtgaggcca
     3121 tcatcgcatg taattttgcc gttggaatta tagttatagc agatgatttt tctttgttcg
     3181 ctgctggagc taaaagaagg atcactgcta ctatagaaaa caccatataa actactacta
     3241 tattgaatat cttcgctgga gcttaaagcc gcatcactac tgctatagaa aatgccatac
     3301 aatgcttcga tctcagaaca ggaactggaa ctaaaagaag gatcgctact actataaaaa
     3361 acaccatata acatagcaga catttgaata tcttcgctac tggaactttc tattgatgaa
     3421 gaacttgggg tttcagtaga tgaactactt tcttgaacac attcttgacc ttcgcattct
     3481 acaggtggat ttgttgtaga atcgctatca caactagacc aaaatgcggt ag
//
LOCUS       DAOOBB010000193         3494 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_29929_length_3494_cov_4.201512, whole genome shotgun sequence.
ACCESSION   DAOOBB010000193 DAOOBB010000000
VERSION     DAOOBB010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29929_length_3494_cov_4.201512"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="PLT31_08095"
     CDS             complement(<1..888)
                     /locus_tag="PLT31_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014544876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HPW95127.1"
                     /translation="MKALLYDTTLRDGNQDRKISLSLADKLQIARLLDSFGIDYIEGG
                     WPNPSNPTDEEFFEKVRELKLSHAKIAAFGSTRRPGIAPQKDPLLQALIKSEAPVKTI
                     FGKSWDLHVTEVIRTRLEENLNMIESSVEYLKKYSEEVCYDAEHFFDGYKENPSYAME
                     TLKAAERGGADFLVLCDTNGGAMPWEVEEITKEIKKYFSTPLGIHVHNDSGLGVANSL
                     YAVKSGADMIQGVFNGYGERCGNANLTTIAANLNFKLQRNFFAKRNISKLRTLSVGID
                     QIVNIPSDVRAPYVGDAAFA"
     gene            951..>3494
                     /locus_tag="PLT31_08100"
     CDS             951..>3494
                     /locus_tag="PLT31_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HPW95128.1"
                     /translation="MNMIKNDLWIGVDVGSTTVKIAIVDPTTTKLLHYSYQRHNAMQA
                     KKVLELLTEAHSLFPDKIFKVAFCGSGGQPFAEATRSFFVQEVVANALAVRATNPETR
                     VAIELGGQDAKVIFFEKDRTTGKLIASDMRMNGVCAGGTGAFIDQVAELLRIKTEVFE
                     SYAARGSKVYEISGRCGVFAKTDIQPMLNQGVAKEDIALSSFHAIAKQTIGGLAQGME
                     IKPPVMFEGGPLTFNPTLVRVFKERLGLTDEQAIVPERSEVLVAWGAALSIGSMFIDK
                     PCDYRQEGSLEALLHFNEKRASERTNKSDLFFKDEADLKSFLARHPMASPHYPRPNSG
                     SVLNVYLGIDAGSTTSKLVLMDENEQVIDGFYASNDGEPLAVLKRALVELTERYEEYG
                     CKLNILGVGTTGYGEQLIAKAIHADYHTVETVAHATAAKHICPDVSFILDIGGQDMKA
                     ISVQDGVVTGITLNEACSSGCGSFIETYARSLGIQMEDIAELAFRAKNPSKLGSRCTV
                     FMNSSIITEQRDGKQPEDIIAGICRSIIENVFTKVIRIRNLNTLGQHVVVQGGTFKNN
                     AVLRAFEQHTGLTPIRPERPGEMGAIGIALLTKHFMEEKRKTNPELQTSFIGLETAKT
                     FSWDNKPGQICQYCTNHCSRTIVTFSDGTSFVTGNRCERGEVTSDPNDPETKKLVAEI
                     HKKMLSVPDMIKRTNQLLVKDYAPAQLIPQSGKTIGIPRALEYWSSLPFWKAFFTSLG
                     YNVVVSKQSDYKLFERGLHSVPSDTICFPAKLVHGHILDLIDKKVDRIFFPMLIALPS
                     DHSQFKATAVCPVVQGYPSVCQNTDAPEKNYGVPMDQPIFHWFNTKMRTQ"
BASE COUNT         1037 a          737 c          663 g         1057 t
ORIGIN      
        1 cgcaaaagca gcatcaccta cgtaaggagc tcgaacatca cttggaatat tgacaatttg
       61 atctatacca acgcttaaag ttctgagttt tgaaatattc cgtttagcaa aaaagtttcg
      121 ttgtaactta aaattcaaat ttgcagcgat cgttgttaaa ttcgcattac cacagcgttc
      181 tccatagcca ttaaaaacgc cctgaatcat gtcagcaccg ctttttacgg cataaagact
      241 gtttgcgact cctaagccag aatcattatg aacatgaatg cctaatgggg tcgaaaagta
      301 cttttttatt tcttttgtga tttcttctac ttcccaaggc atcgctccgc cattggtatc
      361 acaaagaacc aagaaatcag cgccacctcg ttctgcagct tttagtgttt ccatcgcata
      421 ggatggattt tctttatatc catcaaagaa atgttctgca tcataacaaa cttcttccga
      481 gtattttttt aagtattcca cagaagattc aatcatattc agattttctt ctaaacgagt
      541 acgaatcacc tctgttacat gaagatccca gctctttcca aatatggttt tcacaggagc
      601 ttcacttttg attaaagctt gtaacaacgg gtctttttga ggggcaattc ctggtcgacg
      661 agtactacca aaggcagcaa tttttgcatg actaagcttt aattctctga ctttttcaaa
      721 gaattcttcg tctgtaggat tacttgggtt aggccatcca ccttcaatat aatcaattcc
      781 aaaagagtcc aataatcttg caatttgcaa tttatctgct aaagaaagac tgattttgcg
      841 gtcttggttt ccgtcgcgaa gagtggtatc atacaataaa gctttcataa ttgtcaaaga
      901 tagtattttt gcaacttaaa ctttttttta tttgtaaatt tggtctcatt atgaatatga
      961 taaaaaatga tttatggatt ggagttgatg ttggatccac aacagtaaaa attgctattg
     1021 tagacccaac aacaacaaaa cttcttcatt actcttacca gcgccataat gcaatgcaag
     1081 ccaaaaaggt gttggaactt ttaactgaag ctcatagctt atttccagat aaaattttta
     1141 aggtcgcttt ttgtggaagc ggtggtcaac cctttgctga agcgactcgt tctttctttg
     1201 ttcaagaagt cgtagctaat gctcttgcag tcagagcaac taatcctgaa acaagagtcg
     1261 cgatcgaact tggtggtcaa gatgccaaag ttattttctt tgaaaaagac cgaaccacag
     1321 gtaaattgat agcaagtgat atgcgcatga atggtgtttg cgctggtggt acgggagcat
     1381 ttatcgatca agttgccgaa ctccttcgca ttaaaacgga agtgtttgaa tcttatgctg
     1441 ctcgtggctc taaagtctat gaaatttcgg gtcgctgtgg cgtttttgcg aaaaccgata
     1501 ttcaacccat gctcaatcaa ggtgtcgcta aagaagacat cgccctttct agttttcacg
     1561 ccatcgctaa acaaaccatt ggaggccttg cacaaggcat ggaaattaaa cctcctgtca
     1621 tgtttgaagg tggcccatta acattcaatc ccactctagt tcgtgttttt aaggaacgtc
     1681 taggccttac cgatgagcaa gccattgttc ctgaacgctc tgaagttctt gtagcttggg
     1741 gtgccgctct ttctattggt tccatgttca tagacaagcc ttgcgattac cgccaagaag
     1801 gttctcttga agcccttctt cactttaatg aaaaaagagc ttctgaacgc accaataaat
     1861 ctgatttatt ctttaaagac gaagccgatt taaaatcatt cctcgctcgt catccaatgg
     1921 ctagcccgca ttacccacga ccaaattcag gttctgtatt aaacgtttac ctcggtatag
     1981 atgctggttc tacgacatca aaactcgtgt tgatggatga aaacgaacaa gtcatagatg
     2041 gtttttatgc aagtaatgac ggagaacctc ttgccgttct aaaacgtgcg cttgtggaac
     2101 ttaccgaacg ctatgaagaa tatgggtgca aattaaacat cttaggtgta gggacaacag
     2161 gttatggtga acaacttatt gccaaagcca tccatgcaga ctaccataca gtagaaacag
     2221 tagcccatgc cacagcagca aaacacattt gtcctgacgt aagctttatt cttgatattg
     2281 gtggtcaaga tatgaaagcc atttcagttc aagatggtgt tgtcactgga attactctta
     2341 atgaagcttg ttcttctggt tgtggttcgt ttatcgaaac ttatgctcgc tctcttggta
     2401 ttcaaatgga agatattgcg gaactagcct tccgcgctaa gaatccatcc aagctaggca
     2461 gccgttgtac cgtctttatg aatagctcta ttatcacaga gcaaagagac ggaaagcaac
     2521 ctgaagatat tattgcaggt atttgtcgat ctattatcga aaacgttttt accaaagtca
     2581 ttcgaattcg taacctcaat acccttgggc aacatgttgt agttcaaggt ggtacattca
     2641 aaaacaacgc tgttttacga gctttcgaac aacatactgg attaaccccc atccgtccag
     2701 aacgccctgg tgaaatgggt gctattggta ttgctcttct tacaaaacac ttcatggaag
     2761 aaaagcgaaa aacaaatcca gaacttcaaa cctcttttat tggtttagaa accgctaaaa
     2821 cattcagttg ggacaataaa cctggacaaa tttgtcagta ttgtacaaac cattgttcaa
     2881 gaaccattgt gacctttagc gatggtacta gtttcgttac aggaaaccgt tgcgaacgag
     2941 gagaagtcac ctctgaccct aacgatcctg aaacaaaaaa actagtcgca gaaattcata
     3001 agaaaatgct ttctgtgcca gacatgatca aacgcacgaa tcaattactt gtcaaagatt
     3061 atgctccagc acagctaatt cctcaatcag ggaagacgat tggtattcca agagcactcg
     3121 aatactggtc tagtctcccc ttctggaagg cattcttcac ttctcttgga tataacgttg
     3181 ttgttagcaa acaaagtgat tacaaacttt ttgaaagagg cttacatagc gtcccttcgg
     3241 atactatttg cttcccggca aaattagtac acggtcatat tcttgattta attgataaaa
     3301 aagtagatcg cattttcttc ccgatgttga tcgctctacc aagtgatcac agtcaattta
     3361 aagctactgc cgtttgccct gtagtacaag gttaccctag tgtttgccaa aatacagatg
     3421 caccagaaaa aaattatggc gttcctatgg atcagcctat tttccactgg tttaatacta
     3481 aaatgcgcac tcag
//
LOCUS       DAOOBB010000194         3492 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_29947_length_3492_cov_6.793715, whole genome shotgun sequence.
ACCESSION   DAOOBB010000194 DAOOBB010000000
VERSION     DAOOBB010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29947_length_3492_cov_6.793715"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..430
                     /locus_tag="PLT31_08105"
     CDS             143..430
                     /locus_tag="PLT31_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95129.1"
                     /translation="MKIALSKGQGKYIGDLAPFTSFTVSINNTKPTPMTICFGGKTSC
                     SFLITDSFKINLSLNDELLKVLKGFSIITDELIKEVYIDFYEDEFELTQEN"
     gene            432..737
                     /locus_tag="PLT31_08110"
     CDS             432..737
                     /locus_tag="PLT31_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95130.1"
                     /translation="MSAEMSAAIITLIIALAGLIKSFTSSISVDNVVKDREKTKSKRD
                     NDFGIICHRVNELEKFQEKMEAKQNEINNQLFSKLNEINNNVIKIQTIFQEREREKK"
     gene            734..1228
                     /locus_tag="PLT31_08115"
     CDS             734..1228
                     /locus_tag="PLT31_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95131.1"
                     /translation="MNKRLKSLDIKAVYDLQINSSGAAALMSLVELKSLNRRYKLSSD
                     VWIRVVTDKGTISLFAKEGFVFDGRSGSPLLDWYAPNLGSLNERIMWLVHDLLGYATC
                     LDFKTTNKVLRYGLRDQCVYRASKAFMIEKAVSISDDWFGTPKETDWCYNNISKFNVS
                     FFAE"
     gene            1233..1655
                     /locus_tag="PLT31_08120"
     CDS             1233..1655
                     /locus_tag="PLT31_08120"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HPW95132.1"
                     /translation="MTLVQIATARAFSLLDTPYIWGGDLTARTFFGLDCSGLIQLIFE
                     PIGLDPKGDQTAQAFYNYYKNKECKTIQEGCVLFFGQSKDKITHVAYAINTTYMIEAA
                     GGNSKCTSVEIANKLNAKVKVSPIKKRKDLVAVINLFC"
     gene            complement(1644..1868)
                     /locus_tag="PLT31_08125"
     CDS             complement(1644..1868)
                     /locus_tag="PLT31_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95133.1"
                     /translation="MSTYKINKSKQRYNEARERMLLSALDIVNKLKSGVSDEFKKNQL
                     DFKIEARNYKKTIETHLKEELKKLNLSLTK"
     gene            complement(1865..2194)
                     /locus_tag="PLT31_08130"
     CDS             complement(1865..2194)
                     /locus_tag="PLT31_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95134.1"
                     /translation="MTEQFDYCVRSYPGTALEYCADKLTSEQLDWCIRKCPSIALRYC
                     PHKLTKEQFDFYVREWPWVALACCADKLTDEQFDFCVRKDPWAALKYCADKLTEEQKQ
                     YCKRKTK"
     gene            complement(2495..2653)
                     /locus_tag="PLT31_08135"
     CDS             complement(2495..2653)
                     /locus_tag="PLT31_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95135.1"
                     /translation="MKCPNCGFGLQDHEIVREAARINGSKTSPKKKLSSKANGAKKGK
                     KNKETLKT"
     gene            complement(2640..2957)
                     /locus_tag="PLT31_08140"
     CDS             complement(2640..2957)
                     /locus_tag="PLT31_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95136.1"
                     /translation="MKKNKTKEIKYYEFEGKKYFFSDKNLVKNNEDHTILFNDGEQTK
                     KINLLYASTDYFECSNLLERFGVTSEELEQEFPKARRALFLYGIEDINNKIENMENNN
                     EVS"
     gene            complement(2954..3133)
                     /locus_tag="PLT31_08145"
     CDS             complement(2954..3133)
                     /locus_tag="PLT31_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95137.1"
                     /translation="MTTYKINGENPSYALRFGADKLTPEEFDYCVREDPWTALKYCAD
                     KLNKEQLDYCKEKTK"
     gene            complement(3171..>3492)
                     /locus_tag="PLT31_08150"
     CDS             complement(3171..>3492)
                     /locus_tag="PLT31_08150"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HPW95138.1"
                     /translation="CTMNRVELLIEGTIHPTQKPVALYKWLLSRYAKQGDKILDTHGG
                     SGSICIACHDLGFDLTWMELDADYYKAACKRYKDHAAQTVLFEPQEIRQNTNNHELDL
                     EILR"
BASE COUNT         1009 a          618 c          657 g         1208 t
ORIGIN      
        1 cggtgtagcg cctacgggtg caggattaat aaacctaaat ggattatgcg gtgctgtgac
       61 atcagcatca gcgacagctc gtgaaaacct tgtgtcaaag ggctttacgg tcgttgtaaa
      121 tgaaacataa ggagggcaag aaatgaaaat agcactatcg aaaggtcaag gaaaatacat
      181 cggtgattta gctccgttca cgtcttttac cgtcagtata aataacacaa aaccaactcc
      241 aatgactatt tgttttggag gaaaaacttc atgctctttt ttaatcactg attcgtttaa
      301 aattaattta tctttaaatg atgaattgct caaagtgctc aaaggattct ctatcattac
      361 agatgaatta attaaagaag tctacattga tttttatgaa gacgagtttg aattaacaca
      421 ggaaaattaa aatgagtgcg gaaatgtcgg cggctattat tacccttatt attgctttag
      481 ctggattaat aaagagcttt acttcttcta tcagcgttga caacgttgta aaggataggg
      541 agaaaacaaa aagtaagaga gacaatgatt tcggcattat ttgtcatcgt gttaatgagc
      601 ttgaaaaatt tcaagaaaaa atggaagcta aacaaaatga aatcaataat caattatttt
      661 ctaaattaaa tgaaataaat aataatgtca ttaagattca aacgattttt caagagaggg
      721 agagagaaaa gaaatgaaca agcgtctcaa aagccttgat ataaaagcag tttatgattt
      781 acagataaat tcatctggtg ctgctgcgtt aatgagcttg gttgaattaa agtcattaaa
      841 ccgtaggtat aaattatctt ctgatgtttg gataagagta gttacagaca aaggaactat
      901 ttctttattt gcaaaagagg ggtttgtttt tgatgggcgt tcaggttctc cattgctcga
      961 ttggtacgct cctaatttag gctctttaaa tgaaagaata atgtggctag ttcatgattt
     1021 gttgggctat gcgacgtgtt tagatttcaa aactacgaac aaagtattac gttatgggct
     1081 acgtgaccaa tgcgtttaca gagcgtctaa agcatttatg atagaaaaag ccgttagtat
     1141 tagtgacgat tggttcggta cgccaaaaga aaccgattgg tgttataaca acatttctaa
     1201 atttaacgta tctttttttg ctgagtgaca taatgacatt agtacaaata gcaacagcaa
     1261 gagcattttc acttcttgac acgccttata tttggggtgg cgacttaacg gcaagaactt
     1321 tttttggttt agattgctct ggtctaattc aattaatctt tgagcccata ggccttgatc
     1381 ctaaaggcga ccagactgca caggcttttt ataattatta caaaaataaa gagtgtaaaa
     1441 cgattcaaga aggatgtgtt ttatttttcg gacaaagtaa agacaagatt acgcacgtcg
     1501 cttatgctat taataccact tacatgatag aagcagcagg cgggaatagc aagtgcacaa
     1561 gcgtagaaat tgcgaacaaa ttaaatgcaa aagtaaaggt ctctccgata aaaaaacgga
     1621 aagaccttgt tgctgtgatt aatttatttt gttagggata aattaagttt ttttagttct
     1681 tctttcaaat gagtctcaat tgtcttttta tagttacgag cttctatttt gaaatccaat
     1741 tgattttttt tgaactcatc ggaaacacct gactttaatt tattcacaat atcaagggct
     1801 gacaataaca tcctttccct tgcttcgttg tatcgttgtt ttgacttgtt tattttatat
     1861 gtactcattt tgttttccgc ttacaatatt gtttttgttc ttccgttagc ttgtctgcac
     1921 agtatttaag agccgcccat ggatctttac gcacgcagaa gtcgaactgt tcatccgtta
     1981 atttgtctgc acagcatgca agagccaccc atggccattc acgaacgtag aagtcgaact
     2041 gttctttcgt tagcttgtgc ggacagtatc taagagctat gcttgggcac ttacgaatgc
     2101 accagtcgag ctgttcagac gttagtttat cagcacagta ttcaagagct gttccaggat
     2161 acgaacgcac gcagtagtcg aactgctccg tcattagttt ttcagcacaa aatttaagag
     2221 ctattctagg ccactcacga acgaagtagt ttagctgttc gggcgttagt ctgtccgcac
     2281 agtagtcgaa ctgctcttta gttagctcgt ctgcataaaa tcgaagagcc ttccacgggt
     2341 cttttttaac tttttcatct gtcatcattt tgttttctct cctgtttaat ttgttcgcaa
     2401 ttaatacacc tttgagaata tttcttttta cattccttac aacattcatt tctaaacaat
     2461 gttttaatat cctcaaaagt gtaatgaaat cggctcatgt cttaagtgtc tctttgttct
     2521 ttttcccttt tttagctccg tttgctttac tagaaagctt ttttttcggt gaggtcttgg
     2581 aaccgttgat tctagcggct tctcgtacta tctcgtgatc ttgtaatcca aatccacaat
     2641 taggacactt cattattatt ctccatattt tcgattttgt tatttatgtc ttcgattccg
     2701 tataagaaca aagcccttct tgcttttggg aactcttgtt ctagctcttc agaagtgact
     2761 ccaaaccttt ctaataaatt actacattca aagtaatcag ttgatgcata taataaatta
     2821 attttttttg tctgttcacc atcattaaaa agaatggtat ggtcttcgtt attcttcact
     2881 aaatttttat cagagaaaaa atattttttc ccttcaaact catagtattt tatttcttta
     2941 gttttatttt ttttcatttt gttttttcct tacaatagtc tagttgttct ttgtttaatt
     3001 tgtccgcaca gtatttaagt gctgtccatg ggtcttcacg aacgcagtag tcgaattctt
     3061 caggcgttaa tttatctgct ccgaatcgaa gagcatacga tggattttca ccgtttattt
     3121 tataagtagt catttttctt ccttactttg tttaacattg agtttacccg ctagcgtaag
     3181 atttcaagat ctaactcgtg gttattcgta ttttgtctta tctcttgcgg ttcaaataac
     3241 accgtttgag cagcatgatc tttgtaccgt ttgcaggccg ctttatagta gtctgcatct
     3301 agctccatcc atgtcaggtc aaagccgagg tcatggcagg cgatacaaat tgaaccagaa
     3361 ccgccgtggg tgtcgagtat tttatctcct tgttttgcat agcgtgacag gagccatttg
     3421 tacagggcaa cgggtttctg ggtggggtgg atagtgcctt cgatcaatag ttcaacccga
     3481 ttcatcgtgc at
//
LOCUS       DAOOBB010000195         3452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_30417_length_3452_cov_4.774507, whole genome shotgun sequence.
ACCESSION   DAOOBB010000195 DAOOBB010000000
VERSION     DAOOBB010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30417_length_3452_cov_4.774507"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="PLT31_08155"
     CDS             complement(<1..1134)
                     /locus_tag="PLT31_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HPW95139.1"
                     /translation="MTFKCINHEYYLLKGDKILALIRRIFHKCPEFRHNSFKYVARFA
                     PLIPFLGARADLSKNLKRVITFPDHSNSLYLGNPIILAAGANKTAKRIVDYANVGFGG
                     ICVGTATRHFREGNTHRPRIGFLEQDRAIYNSMGLNNEGVEVISKRVDEQLIQAHKKG
                     LCVGVSVAETPGLTDPEEKLKDILESFSIAYKSADYIEINVSCPNTGEDRIDLDTNFA
                     EKVFSNIMEFRNKRGLRKAVYAKLSPDLSERQLMVMMEMLVRNGVNGVVLGNTFPTAQ
                     ISALPMCSNYDDLIALRADGNKGGLSGRPLYENMFWTVSYVKKHYPSISIMACGGIDH
                     GNKIYDLLKLGVDAIQCYSVVAFRWMAAHAMRKELQEVLIANGY"
     gene            1169..2554
                     /locus_tag="PLT31_08160"
     CDS             1169..2554
                     /locus_tag="PLT31_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPW95140.1"
                     /translation="MKNNIEQAFDKLFPHYLNQLKELIKIPSISFDSFDIKEVIRSAE
                     SVKELFEKAGFSHVQFLMPPSGKPSVFAEMQTSKERPTILLYAHHDVQPEMRRELWQS
                     DPFQPEERDGRLYGRGTADDKAGIIVHLAAVSLARELWGKDAPNFKFLIEGEEEAGSS
                     GLEVLLAEHQDLLKADAVIVADLNNIEKGTPCITTSLRGMSAIEVSLTSTETPLHSGS
                     WSGPIPDPAQALCRIITSLTNDNGDILIEGFDEGIKAPSAEELDSYSKLPLKAPQFRK
                     DSRVLEGVQLLVKEPEIPLALWRRPSIVITALESGNRKMAGNVLQNSAYARISIRLAP
                     GMDADDATQKLIDHVQKNIPYGLHATIEKEAGANPFTTNTDHSYFQKMKEAMTEAFEK
                     EAKFIGCGASIPGAILFRNTFGDIPILLIGLEDPDSNPHSENESLDLADFKKTMIAET
                     LFFGKLASINV"
     gene            complement(2670..>3452)
                     /locus_tag="PLT31_08165"
     CDS             complement(2670..>3452)
                     /locus_tag="PLT31_08165"
                     /inference="COORDINATES: protein motif:HMM:NF013753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPW95141.1"
                     /translation="APISVIARWTGLSWSQVDDLTIVHDKATGNVIHVGIGRNTETID
                     NFYEAWQDHLWALQSVSMDLWPAFISSAKKWLKDADSKICFDRFHIAGFFGKAVDQVR
                     KREHHDLKADGNDLLKGTKYDWLRNGNNLDGRSRRWFIKLSRENLKTSRAWAIKETAS
                     MIWKYKSKTWAEKAWRNLFGWMCRSRLSPMVNLAKSLKKHFYGIMNSIMLGIDNGMAE
                     SINSKIQKVKKMACGFRNIERFQNAIMFHSGGLDLYPALPTQ"
BASE COUNT         1029 a          765 c          644 g         1014 t
ORIGIN      
        1 ataaccgtta gcgattaaaa cttcttgtaa ttcttttctc atcgcatgag ctgccatcca
       61 gcggaaagcc actacggaat agcattgaat tgcatctact cctaatttta gtaagtcata
      121 aattttattt ccatgatcaa tcccaccaca agccataatg cttatagaag gataatgctt
      181 tttcacgtag ctcacagtcc agaacatgtt ctcgtataga ggacggcctg aaagccctcc
      241 cttattccca tcagcacgta atgcaatcag atcatcataa ttggaacaca tgggaagagc
      301 ggatatttga gctgtcggga aagtattccc taaaacaaca ccgttcactc cattacgcac
      361 aagcatttcc atcatgacca tcaattgacg ttcagataaa tctgggctta attttgcata
      421 cactgctttt cttaatccgc gtttgtttct gaattccatg atgtttgaaa aaactttttc
      481 tgcaaaatta gtatcgagat cgattctatc ttcgcctgta tttgggcagc tgacgttaat
      541 ttcaatataa tcagcacttt tataagcaat agagaagctt tctaaaatat cttttagctt
      601 ttcttcaggg tccgttaagc caggtgtttc agcaacagag accccaacac agaggccttt
      661 tttgtgagcc tgtatcagtt gttcatcgac tcgttttgaa atgacttcga cgccttcatt
      721 atttaagccc atgctgttgt agatggctcg atcttgttct aagaagccga ttctcgggcg
      781 atgcgtattc ccttctctaa aatgacgagt cgctgtccct acgcaaattc caccaaagcc
      841 cacattcgcg taatccacaa tacgctttgc cgttttattt gcaccggcag ctaaaatgat
      901 tggatttcct aagtatagtg aattgctgtg atccggaaac gtaatcacgc gtttcaaatt
      961 cttacttaaa tcagcacgag cccctaaaaa aggaatcagt ggagcaaaac gtgctacata
     1021 tttgaatgaa ttatgcctaa attcgggaca cttatgaaaa atacgccgaa tgagagctag
     1081 tattttatca ccttttaaga gataatactc gtgattaatg catttaaatg tcatatattt
     1141 aaatttacaa aaaaaaggcc tataaattat gaaaaacaac atagaacaag cttttgacaa
     1201 gcttttcccc cattatctga atcaattaaa agaactgata aaaattccat cgattagttt
     1261 tgattctttt gacataaaag aagtcattcg ctctgccgaa agcgtgaaag aattgtttga
     1321 aaaggcgggc tttagtcacg tccagttttt aatgccacca agcgggaagc cctctgtttt
     1381 tgcggaaatg caaacttcta aagaacgccc cactattctt ctctatgccc atcacgatgt
     1441 gcagccagaa atgagacgcg agctttggca aagtgatcct tttcagccag aagaaagaga
     1501 cggtcgctta tatggccgag gcactgcaga cgataaagca gggattattg tgcatctcgc
     1561 tgccgtttct ttagctcgcg aattatgggg caaagacgct cccaatttca agtttttaat
     1621 cgaaggtgaa gaagaagcag gaagttctgg tttagaagtg ctgcttgcgg agcaccaaga
     1681 tttattaaaa gcagacgcag taatcgtcgc cgacttaaat aacattgaga aaggaacacc
     1741 ttgcattacc acaagtctta gaggcatgag cgcgattgaa gtctctttga cttcgacaga
     1801 aacgcctttg cattctggct catggtctgg cccgattcct gacccagcac aagccctttg
     1861 ccgaatcatc actagcctca cgaatgataa cggagatatt ttgattgaag ggtttgatga
     1921 agggatcaag gcgccgtcag ctgaagaact cgattcttat tcgaaattac cattaaaggc
     1981 acctcaattc agaaaagatt ctcgcgtttt agaaggcgta caactactcg tcaaagaacc
     2041 tgaaattcca ctcgctctct ggagacgccc ttccattgtc atcacggcac tagaatcggg
     2101 caatagaaag atggcaggga atgttttaca aaacagtgct tatgcaagaa tttctattcg
     2161 actcgctccc ggtatggatg cagatgacgc gactcaaaag ttaatcgatc acgttcaaaa
     2221 aaatattcct tatgggcttc atgccactat agaaaaagaa gcaggagcaa atccctttac
     2281 caccaataca gatcacagct atttccaaaa gatgaaagaa gcgatgacag aagcgtttga
     2341 gaaagaagcc aaattcattg gttgtggagc gtctattcca ggagctattt tatttagaaa
     2401 tacctttggg gatattccta ttctactcat tgggcttgaa gatcctgata gtaacccaca
     2461 tagcgaaaat gaaagtttag acttagcaga ctttaaaaag acaatgatcg ccgaaacact
     2521 atttttcggg aaactagctt ctataaatgt ttaattcaac aacacaagtt ttaccatctg
     2581 atctatcatt tcttgactgt attttctgtt tacagagaag gtcttggcat tcggtcatgc
     2641 tcaatacaaa taaatttggg ggcttccaac tattgtgtgg gtaaagcggg atagagatca
     2701 agtccaccag agtgaaacat tatcgcattc tggaaacgct cgatattcct aaacccacac
     2761 gccatcttct tcaccttctg aatcttcgag tttatgcttt cagccattcc attatcaatg
     2821 ccaagcataa ttgagttcat gattccataa aaatgctttt taagtgattt tgcaagatta
     2881 accattggtg ataacctgct tctacacatc cagccaaaaa gattgcgcca cgccttctct
     2941 gcccatgttt tcgatttgta tttccatatc attgatgctg tttctttaat tgcccatgct
     3001 cgtgatgttt ttagattctc acggctaagc ttgataaacc agcgacgtga tctgccatca
     3061 agattatttc cattccgaag ccagtcatat ttagtcccct ttagcaaatc atttccatct
     3121 gcctttagat catggtgttc gcgtttacga acctgatcta cggccttacc aaaaaagcct
     3181 gctatatgga atctatcaaa acatatcttg gagtcagcgt ccttcaacca tttttttgca
     3241 ctgctaataa acgctggcca taaatccata ctcacagatt gcaaagccca taaatgatct
     3301 tgccatgctt cgtagaagtt gtctattgtt tctgtattcc taccaatgcc aacatggatt
     3361 acattcccag tagccttgtc atgaacaatc gtcaaatcat ccacttgact ccatgagagc
     3421 cctgtccatc ttgcaataac actgataggg gc
//
LOCUS       DAOOBB010000196         3417 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_30805_length_3417_cov_5.238548, whole genome shotgun sequence.
ACCESSION   DAOOBB010000196 DAOOBB010000000
VERSION     DAOOBB010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30805_length_3417_cov_5.238548"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="PLT31_08170"
     CDS             <1..741
                     /locus_tag="PLT31_08170"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPW95142.1"
                     /translation="PAIAKKLGELFWPGPLTMVLPKKPCIPDLATSGLSTVAVRLPQH
                     PIAQDIIRQAEVPLAAPSANLFKHVSPTTAEHVADQLGSKIDGIVDGGPCSVGVESTI
                     ISFQNEIPVILRPGAITPLMIESVIGKVILKNSTSNPGEAMLSPGTMDTHYQPHIPLY
                     YGDLPKGYVLPSNTVRLAFGNTVSNIPASLNLSETGNLTEATANLYAFMHKLDVEPNR
                     FILVDPIPNEGLGIALNDRLKRASIKRL"
     gene            complement(746..1423)
                     /locus_tag="PLT31_08175"
     CDS             complement(746..1423)
                     /locus_tag="PLT31_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HPW95143.1"
                     /translation="MAFAADSIFVPKPPEKPLRFCKATSLFLKSIESDSSFVEITRWR
                     DVKFDLRYASFNNVTGHDLYCGSKRAFLHRDAAEKFRKAIRLLKKKYPDYHFVIFDVT
                     RPLYAQEALRALVRGTPFSHYVTSPASGSIHNFGLAIDLSIADSTGNLLDMGTDFDSF
                     EPCAGKQGEAEALKQGRLNQEQIKNRNLLRQVMIQAGFISLPSEWWHFNAYLSKEVRA
                     NYTKVSF"
     gene            complement(1536..>3417)
                     /locus_tag="PLT31_08180"
     CDS             complement(1536..>3417)
                     /locus_tag="PLT31_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95144.1"
                     /translation="SSALFYDGSRAEEVIYHLGRLRVPNMRHLDVGFPGNLSVINPRV
                     LNGIEAHDHYGSGPISQGLAASIQYLPAPVEGEDFGAKLSLGVTLQEIVVEGPFLFWD
                     GFRFAFRRLDEAMLKNMGEKFFTEFKKRDSGSDEVVKSSDPFNLSAYDFFAALSGSDS
                     LGNNWGLHLLYSTDKYIIRQDTSTLMSEVNSIDIIKGSQNYLVLGLDYNSKWGLSWNA
                     GFVRQSLSDTLRDTTGFRANSVAESASFIDGFNFVQTTISVGAEKPFQGHILGSDLST
                     SLLFEHQIIEREYPDFNAVQKMKESGNILTGASRMRWLSQKQNMVLALGALADFSGHA
                     SPIASFDLERKLFSEEKSPWRLFGNAAWRADWTPVYQENDIDGILVKGASAKLGVGYR
                     SKYLDVQTHGFGRYYVNPVLPQPKAYAHYTEQTDVDFAWVYGVQAKAEWKTSHHFSMG
                     TNISSVYGEYELAGDRSLPWQANSRLDMVSHFRFYPRKDSLFSFILSHHAAWHRPLYY
                     YKITPSAIEGGEEVYGTRRVDDIEDFTDLFRTDLRVNLDLPGKRAFFRNVRFYIEANN
                     ILANLDVPALRFLGGENARERSVVTQDNDSNHLNGYEIVPFMAKGMGLFVQFGVEGTF
                     SI"
BASE COUNT         1139 a          738 c          601 g          939 t
ORIGIN      
        1 ccggctattg caaaaaaatt aggagaatta ttctggccag gccctttaac aatggtcctt
       61 ccaaagaagc catgtattcc tgacctggcg acaagcgggc tttctactgt tgcagtaagg
      121 cttccgcaac accctatagc tcaagatatt attcgccaag cagaagtgcc tcttgcggct
      181 ccgagtgcaa atttattcaa gcacgtaagc cctaccacag cagaacacgt cgcagaccaa
      241 ttaggttcta agatcgatgg gattgtcgat ggaggccctt gttctgttgg tgttgaaagc
      301 acgattattt cttttcaaaa tgaaatacct gtaattctaa ggcctggtgc aattacacca
      361 ttaatgattg aatctgttat tgggaaagtg atcttaaaaa attccacatc aaatccagga
      421 gaagcgatgc tctctccagg aacgatggat actcattacc agcctcatat tcctctttat
      481 tacggcgatt taccaaaggg ctatgttctt ccttcaaata cggtacgcct tgcttttgga
      541 aacaccgttt caaacattcc agcctcatta aatctttctg aaacaggcaa tctcacagaa
      601 gccacggcca atttatacgc gtttatgcat aaattagacg tagagcccaa tcgttttatt
      661 ttagtagatc ctattccaaa tgaaggatta ggaatcgcat taaatgatcg gctgaagcga
      721 gcttctataa aacgcctttg attccttaaa aagaaacttt ggtgtaatta gcccgtactt
      781 ctttagagag ataagcatta aaatgccacc attcacttgg taatgaaata aaaccagctt
      841 gaatcatcac ctgacgaagt agatttctgt tctttatttg ctcttgattt aagcggcctt
      901 gttttaacgc ctcggcttcg ccttgtttac ctgcacatgg ttcaaaagaa tcaaaatctg
      961 tacccatatc taaaagattc cctgtactat ctgcgatcga taaatctata gctaaaccaa
     1021 aattatgaat actgcccgat gcagggcttg tgacataatg agaaaaaggc gttcctctca
     1081 ccaatgccct aagcgcttct tgagcatata aagggcgagt gacatcaaaa atcacaaaat
     1141 gataatcagg atattttttc ttcaatagac gtatagcttt cctaaatttt tcggcggcat
     1201 cacgatgtaa aaaagcacgt ttagaaccac aatataaatc atgccccgtc acattattaa
     1261 aagaagcata tcttaaatcg aatttaacat ctcgccatct tgttatttca acgaaagatg
     1321 aatcagattc tattgatttc aaaaaaagtg acgttgcttt acaaaagcgt agaggttttt
     1381 ctggtggttt agggacaaaa atcgaatccg cggcgaaagc catgaacgga aaaaaaacca
     1441 atagaaagaa tagattcaaa aatcttaaag aaaatccaaa attagacgat ctaaaaagca
     1501 tcataaaaaa ataattctaa aacaagctta tgaacttaaa tactgaaagt gccttctaca
     1561 ccaaactgga caaagagccc cattccttta gccataaaag gaacaatttc atacccattt
     1621 agatgattag aatcattatc ttgagtgacg acagagcgtt cgcgtgcatt ttcaccgcct
     1681 aagaagcgaa gtgcaggcac atctagatta gctaagatat tgtttgcctc aatataaaaa
     1741 cgaacattcc taaagaaagc tctttttcca ggaaggtcta agttcactct gagatcggtt
     1801 ctaaataaat ccgtaaaatc ctcaatatca tctacccgtc ttgtaccata gacttcttca
     1861 ccgccctcga tcgctgatgg ggtaatcttg taataataaa gtggcctatg ccaagccgca
     1921 tgatgagaga gaataaaaga gaatagagaa tcttttctag ggtaaaagcg gaaatgagag
     1981 accatgtcta aacgcgaatt cgcttgccat ggaagagagc gatcccctgc aagttcatac
     2041 tctccataga ccgaactaat atttgttccc atagaaaagt gatgagatgt tttccactct
     2101 gcttttgcct ggacaccata gacccatgca aaatcaacgt ccgtttgttc tgtataatga
     2161 gcgtatgcct taggttgtgg aagtacaggg tttacataat agcgaccaaa gccatgtgtt
     2221 tgaacatcta aatacttgct tcgataacca acaccaagtt ttgcagaagc tccttttaca
     2281 agaataccat ctatatcatt ttcttgatat acaggagtcc aatcagctct ccatgctgca
     2341 ttaccaaaaa ggcgccaagg gcttttttct tctgaaaata atttgcgttc caaatcaaaa
     2401 gaagcaatag gcgatgcatg gccactaaaa tcagcaagag cccctaaagc aagaaccata
     2461 ttttgttttt gagaaagcca cctcatacga gaagcccccg tcaaaatatt accactctct
     2521 ttcatctttt gtacagcatt aaaatcagga tattcacgtt caataatttg atgttcaaat
     2581 aaaagagaag tacttaaatc agaaccgaga atatgaccct gaaaaggctt ttcagcaccc
     2641 acactaatcg tcgtttgaac aaaattaaag ccatcaataa acgatgcaga ttccgctaca
     2701 gaatttgctc taaagccagt cgtatcacga agcgtgtctg acaaggactg ccttacaaat
     2761 ccagcattcc aactgagacc ccattttgag ttatagtcta agccgagtac taaataattc
     2821 tggcttcctt taataatatc aatactattg acttcactca ttaaggtaga ggtatcttga
     2881 cgaatgatat atttgtctgt gctatataat aagtgaagtc cccagttatt gcctaaagaa
     2941 tcagatccac tcaaagcggc aaaaaaatca taagcactta aattaaaagg atctgaagat
     3001 tttacaactt catcagagcc actatccctt ttcttaaact cagtaaaaaa cttttcgccc
     3061 atatttttga gcatggcttc atctaaacga cggaatgcaa aacggaaccc atcccaaaaa
     3121 agaaaagggc cttccaccac aatttcttgt aatgtaaccc ccaaggagag tttggctcca
     3181 aaatcttcac cttctactgg tgcaggtaag tattgaatag aagcagcgag cccctgacta
     3241 ataggcccag atccataatg gtcatgtgct tcaattccat ttaaaacacg aggattgatc
     3301 acagataaat taccagggaa tccaacatca agatgacgca tattaggaac acgtaaacga
     3361 cctaaatgat aaatcacttc ttcggctcta gatccatcat aaaagagggc actagaa
//
LOCUS       DAOOBB010000197         3404 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_30952_length_3404_cov_3.978501, whole genome shotgun sequence.
ACCESSION   DAOOBB010000197 DAOOBB010000000
VERSION     DAOOBB010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30952_length_3404_cov_3.978501"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..2958
                     /locus_tag="PLT31_08185"
     CDS             133..2958
                     /locus_tag="PLT31_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPW95145.1"
                     /translation="MNTIVDLNGEWELLWDTEDIGITNRWYANPQEGCEKVQVPHIWE
                     QSFEKMFLTQDTAYYFKRFFVEEKQVPKRIFLCFERIATHAVIWLNGKLIGEHFGAYS
                     SFIVETSKAIKLGEENILCIRVANMGAANSRIDFGRNSKEGVEDRYVHPTEMPVGLPW
                     TQYPFAGIYGNVSLILGNAAFISGMQVEPDMDQERVAVEVSFNNPRGFQTRLRVLMRN
                     PAGEVSEIYKELKLEKENASHRFILGIKDWKKEKYTWAHDKPNLFAIEVQLESKTTKT
                     KGIPEYSFSVMKTFGFRKFDCIKGDFYLNDSIIKLHGINYSQHWSQGGLWTSQNPNFR
                     KDLESLKKLGFNTIRSGGAPLSNEALDLCDELGLLVFQEFPVHTMRSSARGLEEVRSV
                     IEDIVTEQKHHPSIVAWVIGAENGTLVLENGNKLLKNVDLVDMSRPVISNLNCVYLDN
                     EESFHKDTGKLMGVTNDRILLYASHRLHLRMNPSANLSYFLSLYCSKEETEVSIPDST
                     LGDSLFQDDYESFVKETNGKILVTLKNHTLLPKEPTALTGPRSNKNAKAIKSLYKQIN
                     AFIADKNISIWPSFKAFNEDAQRIAIKSKIDQLTSIQSNSLVSGYFLDQWADIGTDFS
                     GLVDENRKSKGIEAFAKEITTPTKLLISGFEHVVVPQNEISFQMVLLNHDRLEDFETE
                     IVIVDKAGQIVSSQKKNEKGQTSLTPLGIYTIKAPKSTGDYTVQAKLIYKGKEIYQTT
                     ESLIVIEQADVKAAMKQVCFLDNSTETSDALASLKGSEQVIFTANLSSWNNEILAKIV
                     DVTKNGGKTLLLSDMTQEDIEMFNSSHHFDSIIESHFSTGANELSLHYLTEGSPLLPI
                     FNGQSVLDHNASAVMPSVSLNELEGSTVLARSVSIIKGEVKTGVDLQMLPFGKGKIVF
                     NQFSVFEGLETSALADALFCKIVKLLV"
     gene            complement(3059..>3404)
                     /locus_tag="PLT31_08190"
     CDS             complement(3059..>3404)
                     /locus_tag="PLT31_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95146.1"
                     /translation="DIQHYLYNSKNWFFERESRKIWLREWFALFNSYYDLWGVDTLEL
                     DSLKLPEVKSLKEERISLPKVESKKWISIKETFLKNHVSDSILKVDSAKTDSTKKDST
                     KIKSAKKDSTSR"
BASE COUNT         1143 a          593 c          587 g         1081 t
ORIGIN      
        1 atgaacatct agtaaaaaga gtagatgttc caccagaata aaccaaaagc gagtcaaaat
       61 aaaaggctcc gtatataaaa agtacttatt atttactaaa tttaggccat ctattacaaa
      121 aggacttatt gaatgaatac aatcgtagac ctcaatggcg aatgggaact cctttgggat
      181 accgaagata ttggcataac taaccgttgg tacgcaaatc cccaggaagg atgcgaaaaa
      241 gtacaagtac ctcatatttg ggaacaatct tttgaaaaaa tgtttttaac tcaagatacc
      301 gcttattatt tcaagcgctt ttttgttgaa gaaaaacaag ttcctaaaag aatttttctt
      361 tgttttgaac gtattgcgac ccacgccgtt atttggttaa acggaaaatt gattggtgaa
      421 cattttgggg cctattcttc ttttatagta gaaacatcaa aagcaattaa actcggtgaa
      481 gaaaatattc tttgcatccg tgtagctaac atgggagcag ctaatagccg tattgatttt
      541 ggtcgcaact ccaaagaagg tgttgaagac cgctatgttc atcctacaga aatgccagtc
      601 ggtttacctt ggacgcaata tccttttgct ggtatttatg gtaatgtttc tttaattctc
      661 ggtaatgcag cctttatatc tggcatgcaa gtagagcccg atatggatca agaacgcgtt
      721 gccgtagaag tctcttttaa taatcctaga ggtttccaaa caagacttcg tgttttaatg
      781 aggaatccag caggggaagt atccgagata tataaagaat taaaactcga aaaagaaaat
      841 gcttctcatc gttttatttt aggaattaaa gattggaaaa aagaaaaata tacttgggct
      901 catgataagc caaatctatt tgccattgaa gtccagttgg aatccaaaac aacaaagact
      961 aaagggattc cagaatattc attttctgtg atgaaaacct ttggattcag aaaatttgat
     1021 tgcattaaag gtgattttta cttaaacgat tctattatca agcttcatgg tatcaattac
     1081 tcccagcact ggagtcaagg cggattatgg acctcacaaa atcctaattt ccgtaaagac
     1141 ttagaatcat taaagaaact tggttttaat accattcgtt ctggtggagc ccctctttca
     1201 aatgaagctc tagacttatg tgacgaactt ggtctcttag tcttccaaga atttcctgtt
     1261 cacaccatgc gttcttctgc tcgcggatta gaagaagtac gttctgtaat tgaagatatt
     1321 gtaactgaac aaaagcacca tccttctatt gtcgcttggg tcattggtgc cgaaaatgga
     1381 acccttgtat tagaaaatgg aaataaatta ttgaaaaatg tggatctagt cgatatgagt
     1441 agaccagtga tcagtaatct aaattgcgtt tacttagaca acgaagaatc tttccataaa
     1501 gatacaggta aactcatggg cgtaacaaac gatcgtattt tattatacgc atcacatcgc
     1561 cttcatttac gtatgaatcc aagtgccaac ctcagttatt tcttgtctct atattgcagt
     1621 aaagaagaaa ccgaagtttc cattccagat tcaactcttg gtgacagttt atttcaagat
     1681 gactatgaat catttgtaaa agaaactaat ggaaaaatct tagtcacatt aaaaaatcat
     1741 actctattac caaaagagcc tacagctctc actggccctc gcagtaataa gaatgcaaaa
     1801 gcgattaaaa gcctttacaa gcaaattaat gctttcatag cagataaaaa tatttctatc
     1861 tggccttcat tcaaggcttt taatgaagac gctcaacgaa tcgccatcaa aagcaaaata
     1921 gatcagctaa cttctataca aagtaattct ttagtgtctg gttatttctt agatcaatgg
     1981 gctgatattg gaactgattt ttctggttta gttgatgaaa accgtaagtc aaaaggcatt
     2041 gaagcttttg caaaagaaat caccactcct actaagttac tgatttcggg ctttgaacat
     2101 gttgttgtgc ctcaaaatga aatttcattc caaatggttc ttctcaacca tgatcgttta
     2161 gaagattttg aaactgaaat tgttattgtg gataaagcag gtcaaattgt ttcttctcag
     2221 aagaaaaatg aaaaaggcca aacaagcctt accccgcttg gcatttatac aattaaagca
     2281 cctaaatcaa caggcgatta tactgttcaa gcaaaattaa tttataaagg taaagaaatt
     2341 taccaaacga cagaaagctt aattgttatt gaacaagcag acgtaaaagc agccatgaaa
     2401 caagtatgct tcttagataa cagtacagaa actagtgatg cacttgcttc actaaaagga
     2461 tctgaacaag tcatctttac agctaattta agctcatgga ataatgaaat tttagcaaag
     2521 attgtggacg tcaccaaaaa tggtggtaaa acacttttac tttcagatat gactcaagaa
     2581 gatatcgaaa tgtttaattc tagtcatcat tttgattcca taatcgaatc tcatttttct
     2641 acaggcgcaa atgaactcag tttacattat ttaactgaag gctcccctct tcttcctatt
     2701 tttaatggcc agtctgttct agatcacaat gcttctgctg taatgcctag cgtttctcta
     2761 aacgaactcg aaggttcaac tgtattagca cgttcagtct ccatcattaa gggcgaagta
     2821 aaaacaggtg tagatttaca gatgttgcct tttggaaaag gaaaaattgt atttaatcaa
     2881 ttcagtgttt ttgaaggtct tgaaacaagt gcattagctg atgcgctctt ctgtaagatt
     2941 gtcaaacttc tagtttaatc aataattctc ttattaaaaa gctcctctaa ttagaggagc
     3001 tttttttatc tcgtttaaag aaatttaata tgaactctct agaagagtta ttcaaccatt
     3061 aacgcgacgt agaatctttt tttgcagatt taatttttgt agagtccttt tttgtggaat
     3121 ctgtttttgc agagtctact tttagaatcg aatcagagac atgatttttt aagaaagttt
     3181 ctttaataga aatccatttc ttagactcta cttttggaag agaaatacgt tcttctttta
     3241 aggattttac ttctggaagt tttaaagaat ctagttctaa tgtatctaca ccccacaaat
     3301 cataatagga attaaaaaga gcaaaccatt cacgtaacca tatttttctt gattcccttt
     3361 caaaaaacca atttttagaa ttgtaaagat aatgctgaat atct
//
LOCUS       DAOOBB010000198         3403 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_30967_length_3403_cov_3.921744, whole genome shotgun sequence.
ACCESSION   DAOOBB010000198 DAOOBB010000000
VERSION     DAOOBB010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30967_length_3403_cov_3.921744"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /locus_tag="PLT31_08195"
     CDS             complement(<1..1259)
                     /locus_tag="PLT31_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014544989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HPW95147.1"
                     /translation="MAKKMIAVDGNEATANIAHKLSEVIAIYPITPSSPMAEHSDAWS
                     AVGKKNLWGQVPSVFEMQSEGGAAGAVHGALQAGALTTTFTASQGLLLMIPNMYKIAG
                     ELIPTVFHVTARSLAAQGLSIFGDHSDVMACRQTGFAMLASASVQECQDMALIAHAAT
                     LKSRVPMMHFFDGFRTSHEVMKIEAIDDTIIRDMIDEKLVKACRDRSLTPDRPTIRGT
                     AQNPDVYFQGRETVNPFYNALPEIVQNYMDQLATHTGRQYKLVDYKGAKDAERVIVIM
                     GSGADTVEDTVNHLVAQGEKVGVLNIHLYRPFPMDAFMAALPKTVKTIAVLDRCKEPG
                     SAGEPLFQDVLTAVTESVMAGKSTLPKIIGGRYGLSSKEFTPAMVKAVFDECAKKDPK
                     ARFTVGINDDVTHTSLTVDPSFKLQKK"
     assembly_gap    1931..2030
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2366..>3403
                     /locus_tag="PLT31_08200"
     CDS             2366..>3403
                     /locus_tag="PLT31_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HPW95148.1"
                     /translation="MTKQALRYNHLGYAPFAPKKFLVVETSLTSFEIKNEYEEVVFQG
                     KLEPKGVWSFTEESCFLGDFSAFQKKGKYRIVVSDGLQSDWFLIEDEWLCRQLNANIK
                     SFYYQRSGVELPPKEAGKWARPAAHLDDAITFHPSMERPGIWNAHGGWYDAGDYGKYI
                     VNGGVSVASLLLACELYPKITEKTKIYEEIRFELDFFLRMQDHDGGVFFKITPDRWDS
                     FLPPSKTIYERRVLGKSTTSTLNFAGVMAHAHRIYANQDPVFAAHCLEKAKKAYFWAK
                     NNPNVPFPNNTEGSGPYGDENFSDEFFWARSMLFRETFGSDFSDKDFIKDLEQDIETQ
                     GVLLDINWRDTQ"
BASE COUNT         1047 a          632 c          648 g          976 t
ORIGIN      
        1 aacttctttt gaagcttaaa tgaagggtct acagttaaac tggtatgagt aacgtcatcg
       61 ttaataccaa cagtaaaacg agcttttgga tcctttttag cgcattcatc aaatacagct
      121 ttgaccatgg ctggtgtaaa ttctttagaa gataaaccat aacggccacc gataatctta
      181 ggaagagtgg atttaccagc catcacagat tcggttacag cagtcaaaac gtcttggaat
      241 agaggttcgc cagcagagcc tggttctttg caacgatcaa gaacagcaat ggtttttact
      301 gtttttggaa gagcagccat aaaggcatcc attgggaatg gtctataaag atgaatgttg
      361 agaacaccca ctttttcacc ttgagcaact aaatgattca cggtgtcttc aacagtatct
      421 gcaccagaac ccataatcac aatcactcgt tccgcatctt tagcaccctt gtaatcgacc
      481 aatttatatt gacgaccagt atgagtagct aattgatcca tgtagttctg gacaatttca
      541 ggaagtgcat tgtagaatgg gtttacggtt tcacgacctt ggaaataaac atcaggattt
      601 tgagccgtac cacgaatcgt aggacgatct ggagttaagc tgcgatcacg acaagccttt
      661 acgagctttt cgtcaatcat atcacgaata atggtgtcgt caatggcttc gattttcatg
      721 acttcatgag aagtacggaa accatcaaag aagtgcatca ttggaacgcg gctttttaaa
      781 gtagcagcgt gagcaatcaa agccatatct tgacattctt gaacagaagc agaggcaagc
      841 atcgcaaaac cagtttggcg acaagccatc acgtcggaat ggtcaccgaa aatggataag
      901 ccttgagctg caagagaacg agcagtaaca tggaaaacag taggaattaa ttcacccgca
      961 atcttgtaca tattggggat catcaataga agaccttgag aagctgtaaa agtagtagtt
     1021 aatgctccag cttgtaatgc accgtgaacg gcacctgcag cgccaccttc ggactgcatt
     1081 tcaaaaacag atggtacttg accccagaga ttctttttac caacggcact ccatgcatcg
     1141 gaatgttcgg ccattgggct agatggggta ataggataaa tagcaatcac ttcactcaat
     1201 ttgtgagcaa tattcgcggt agcttcattg ccgtctaccg caatcatttt ctttgccatg
     1261 gacatctcca ttaatgtggt tcaataaact tgcactaaag gtagataatc taacttgaat
     1321 tggttataaa aaaactaaaa ataccctagt tttgatctct tttttaaaga aaaaggatta
     1381 gggggttgag ggaatatatt tgctaagcta tttggaattt tttaccccct ctttttaaga
     1441 gcaagaaaaa gagatgggtg ttttatacga gtacgtttta tatgagttcg atttatatga
     1501 gttcgattta tatgagtgct ttaatattag cttgttaata tgattgtttt ttaataaata
     1561 gggattcaat aagtaagggt gatgtgtatt aataattcgt gatgttgtaa taattcgtat
     1621 tgatgtaaag gcgaaatcaa agccaaccat tttttaatga caaacgtttt tatttattaa
     1681 aaatgcaaaa tgagtgcaat tccaataaaa gcttctttga taaaaatcgc tgtgccccca
     1741 aaaaaacctt taatcaattc aatgcattaa tccaattttg ttgcctaaat ccatgaatca
     1801 aatccaattt tgtttattaa aacagcaaaa cctattccat ccaatttatc caaaaaaaac
     1861 aaagtaaaac ataatccaat acggcaatat ttgtttctca ccgaaggggc gtaatgtttt
     1921 tatatttcaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccaatacggc
     2041 aatatttgtt tctcaccgaa ggggcgtaat gtttttatat ttcaagaggt tatagaaaat
     2101 tacattccca aaagccacaa tcaatccaat cctttcatta atccaatttt gttgccctga
     2161 tctgtgaaac caatgccatc gaatatctcg tcaaatttgt ttcccaaatc ggcgaaagca
     2221 atgccaatga cttctcttaa aaaaaggtgc tcagtaaatt ggggaagctt ttataaaacc
     2281 catataaaat agcttggttt caattgtata aagatttctc taatacagct ccatttttgg
     2341 gacaaaaaaa ttctattctt gattcatgac aaaacaggct ctgcgttata atcatctcgg
     2401 ctacgctcct tttgcaccaa aaaaatttct ggtcgttgaa acctcattga cttcttttga
     2461 aatcaaaaat gaatacgaag aagttgtttt tcaaggaaaa ctggaaccta aaggcgtttg
     2521 gtcttttaca gaagaatcct gctttttagg cgatttttcg gcatttcaaa aaaaagggaa
     2581 gtaccgcatt gtcgtttctg atggtttaca aagcgattgg tttcttatcg aagacgagtg
     2641 gttgtgccgt caattaaacg cgaatatcaa gtctttttat taccaaagaa gtggggtcga
     2701 attacctcca aaagaagcgg gcaaatgggc tcgtcctgca gctcacttag acgatgcgat
     2761 tacttttcac ccgagcatgg aacgtcctgg aatctggaat gcgcatggcg ggtggtatga
     2821 tgcaggcgat tatggaaaat acattgtgaa tgggggtgtt agtgtcgcct ctcttttact
     2881 ggcctgcgaa ttatacccca aaataaccga aaaaacgaaa atttatgaag aaattcgatt
     2941 tgaattagac ttctttttaa gaatgcaaga tcacgatggt ggcgttttct ttaaaatcac
     3001 tcccgaccgt tgggattctt tccttccacc ttcgaagacc atttacgaaa gacgcgtttt
     3061 aggaaagtcc acgacttcga ccttaaactt cgctggggtc atggctcatg cgcatcgcat
     3121 ttatgcaaat caagatcctg tgtttgcagc acattgcctc gaaaaagcta aaaaagccta
     3181 tttttgggcc aaaaacaatc caaatgtacc ttttcccaat aacacagaag ggagtggtcc
     3241 ttacggcgat gaaaactttt cggacgaatt tttctgggca agaagcatgc tctttagaga
     3301 aacgtttggc tccgattttt cagataagga tttcataaaa gatctagagc aagatatcga
     3361 aactcaaggg gtattactcg acatcaattg gcgagatacg caa
//
LOCUS       DAOOBB010000199         3380 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_31258_length_3380_cov_3.617143, whole genome shotgun sequence.
ACCESSION   DAOOBB010000199 DAOOBB010000000
VERSION     DAOOBB010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31258_length_3380_cov_3.617143"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="PLT31_08205"
     CDS             complement(<1..800)
                     /locus_tag="PLT31_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HPW95149.1"
                     /translation="MKKIIYLAMSILLAGASFSFAQAEGMFTGSLPENWSADVVASVK
                     YSRNHFSNWKDGGVNTNTWLLQYDADLKGKWKVANWRNLVNLAWGQTYTEGVGTRKSL
                     DKIFIESLVEFNPFEKLKPYAGVRYETQFAKGYEYTDSTKTPVSSFMDPCYLTQFAGI
                     SYAFTEDFTQRLAFANRMTISKGYGYADDGSTTKFESFKDEPGLESVTEFKHSFSTIM
                     TFKTRLWAFVNFKGVDEIDGRWENALLISLTPYLEFSLGIDLAYDKDF"
     gene            complement(855..2000)
                     /locus_tag="PLT31_08210"
     CDS             complement(855..2000)
                     /locus_tag="PLT31_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3410 domain-containing protein"
                     /protein_id="HPW95150.1"
                     /translation="MSRRLHIVIDDAIPFIRGVLEPFFDVSYIPGIEITNQNLKNAEG
                     LVVRTRTICDSSLLNQTPVRFVATATIGTDHLDTLYLNQQGIQWMHAPGCNALSVCQY
                     VLAAIFEILYETKKPLTETTLGIVGVGHIGSRLYPLAKALGIHCLLCDPPRADEDKEN
                     DYLSLMEVLSQSDIVSLHVPLIQQGLYPTQHLINDTHLKKMPSHAYLINSSRGPVVDN
                     QALSYHLKEKTIAGAILDVWEEEPNLNKTLLNQVMIGTPHIAGYSQDGKANATSQVVR
                     GIGNAFDIEPLKTFDLHLEKQTLEIEPLVEDDFLSLRAIFKKTYDIRKDSLLLKHSPD
                     NFETFRKNYPVRREPRAYELKTLIKKENIRRVLEKLGFHLCVDSHFV"
     gene            2035..2553
                     /locus_tag="PLT31_08215"
     CDS             2035..2553
                     /locus_tag="PLT31_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95151.1"
                     /translation="MKVLFYLIFLFIGFSFATEKAPTSKWTPEIGISIGSPAPLFLSV
                     GLHYQFLNKVGAFIRTEGLGSHTRKNDYWCGARGAIGFSLFRDQPFFLESGVSVGYFY
                     ARAPNRIQQIFNEINQGYYFYEYDWKEFYDVSIMIGVHLWGIHVRTELPLVDQGNTSY
                     SLLWRVGYLWSF"
     gene            complement(2564..>3380)
                     /gene="trpS"
                     /locus_tag="PLT31_08220"
     CDS             complement(2564..>3380)
                     /gene="trpS"
                     /locus_tag="PLT31_08220"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPW95152.1"
                     /translation="YKVAATWLALGLNPEEGLFYKQSDIPEIFELSWVLSCFTPKGFM
                     NRAHAYKDKVNKNVALGEDSDSNVNMGLYCYPCLMDADILMFNANVVPVGKDQKQHVE
                     FARDIAIRFNKKYGEILTVPEPLIQETTDVIPGLDGRKMSKSYDNTIEIFLDSKTLKK
                     KIGKIVTNAQTIEEPKDPDTCNVFALYKLFASPEQVSVLAERYRAGGLGWGHAKAELQ
                     AVLEEHLGPSRDRYFYLLSHTEEIDKILAYGAEKARVRSKETMSRVRDVLGFF"
BASE COUNT         1047 a          663 c          577 g         1093 t
ORIGIN      
        1 ctgaaatctt tgtcataagc taggtctatt cctaaagaga attcaagata tggtgttaaa
       61 gaaattaaaa gtgcgttttc ccaacgacca tcaatttcat ctactccttt gaaattcaca
      121 aatgcccata gacgagtttt gaatgtcata atagtagaga atgagtgctt aaattctgtg
      181 acactttcta aaccaggttc atctttaaag gattcaaatt tagtggtaga gccatcatca
      241 gcatagccat agcctttaga aatagtcata cgattagcaa atgctaaacg ttgagtaaaa
      301 tcttctgtaa aagcgtaaga aataccagca aattgagtta agtaacaagg atccataaaa
      361 gaagaaacag gtgtttttgt ggaatcggtg tattcatagc cctttgcaaa ttgagtttcg
      421 taacgaacac cagcataagg ttttaatttt tcaaaaggat taaattcaac taagctttca
      481 ataaaaatct tatccaaaga cttacgagtt ccaacgcctt cagtataagt ttggccccat
      541 gccaagttca ctaaattacg ccagttagca accttccatt ttcctttcaa gtctgcatcg
      601 tattgcaata accaggtatt tgtattcacg ccaccatctt tccaattaga aaaatgattt
      661 cttgaatact tcacagaggc aacaacatca gcactccaat tttctggaag agagcctgta
      721 aacattcctt ctgcttgagc aaaggagaag ctcgctcctg caagcaaaat agacatggcg
      781 agataaatta tttttttcat aaaaaatgat tccttttgtt agttaactca aaaatagcac
      841 aatttatttt gaattcaaac gaaatgtgaa tctacacata aatgaaaacc taacttttct
      901 aatacccttc gaatattttc tttttttatt agtgttttta attcataagc acgtggttca
      961 cgacgaacag gatagttttt tcggaatgtt tcaaaattat ctggactatg ctttaacaat
     1021 aaactgtctt ttcttatatc atatgttttc ttgaaaatgg ctcgtaaact caaaaaatca
     1081 tcctcaacca gaggttctat ttctagagtt tgtttttcta agtgcaaatc aaatgttttg
     1141 aggggttcta tatcaaaagc atttccgatt cccctcacca cttgacttgt cgcattagcc
     1201 tttccatctt gactatatcc tgcaatatga ggcgttccaa tcatcacttg attcaaaaga
     1261 gtcttattca aattgggttc ttcttcccac acatctaata tagctcctgc aattgtcttt
     1321 tcttttaagt gataagacaa agcttgatta tcaaccactg gccctcttga actattaatc
     1381 aaataggcat gtgaaggcat ttttttaagg tgagtatcat taattagatg ttgtgttgga
     1441 taaaggcctt gttgaataag gggcacatgt aaagagacta tatcactttg agaaagcact
     1501 tccattaatg acaaataatc attttcttta tcttcatcgg cccgtggagg atcacaaagc
     1561 aagcaatgta tgccaagagc ttttgctaat ggatagagac gagaaccaat atggcctacc
     1621 cctacaatcc ctaatgtcgt ttctgtgaga ggtttctttg tttcgtaaag aatttcaaat
     1681 atggccgcaa gaacatattg gcaaacactc agagcattac aaccaggggc atgcatccat
     1741 tgaattccct gttgatttaa gtaaagagta tctagatgat cggtgcctat agtagcagta
     1801 gctacaaaac gaacaggagt ttgatttagt aaagatgaat cgcatatagt tctagtccga
     1861 accaccaacc cctctgcatt tttcaaattc tgattcgtta tttctattcc aggtatatag
     1921 gatacgtcaa aaaaaggctc cagtacccct cgaataaaag gaatcgcgtc atcaatcaca
     1981 atatgtaaac gtcgagacat gatcagaata tagtctttct ataattgttt gcaaatgaag
     2041 gtgttatttt atttaatttt cttatttatt ggcttttctt ttgccacaga aaaagcccct
     2101 actagcaagt ggactccaga aataggtatt tcaattggtt ctccagctcc attattttta
     2161 agtgtcggcc ttcattatca gttcttaaat aaagttgggg cctttatccg aactgaagga
     2221 ttaggttctc acacaagaaa aaatgattac tggtgtggag cgagaggggc tattggtttt
     2281 tctttatttc gagatcaacc gttcttttta gaatcaggtg ttagcgtcgg ttacttttac
     2341 gcacgtgccc caaaccgaat tcagcaaata tttaacgaaa ttaatcaagg ctactatttt
     2401 tatgaatacg actggaaaga gttttacgat gtttcaatca tgattggggt tcatctttgg
     2461 ggtattcatg tccgaacaga actacccctc gtggatcaag gtaatacttc ctactctctt
     2521 ttatggagag taggctatct ctggtctttt taaaaatgat cgactaaaag aagcctaaga
     2581 cgtctctgac tcgagacatg gtttccttag aacgcaccct tgccttttcc gctccataag
     2641 caagaatttt atctatttcc tcagtatgag aaagcagata aaaatagcgg tcacgcgatg
     2701 ggcctaaatg ttcttctaat accgcttgta actctgcttt ggcatgtccc caacccaaac
     2761 cgccagcacg ataacgttct gcaagaacgc tcacttgttc tggagaagca aacaatttat
     2821 acaaagcaaa aacattacaa gtatctggat ctttaggctc ttctattgtc tgagcattag
     2881 tgacaatctt tcctattttt ttctttaggg tttttgaatc caaaaaaatc tcaatcgtat
     2941 tgtcataaga tttactcatc ttgcggccat ctaaaccagg aatcacatct gttgtttctt
     3001 gtattagtgg ttctgggaca gtgagtattt ctccatattt tttattaaaa cgaatagcga
     3061 tatctcgagc gaattcgaca tgctgtttct gatcttttcc aacaggaacg acatttgcgt
     3121 taaacattaa aatgtctgca tccattaaac atgggtagca ataaagcccc atatttacat
     3181 tgctatctga gtcttctcct aaagcaacat ttttattcac tttatcttta taagcatgcg
     3241 cccgattcat aaagcccttt ggtgtaaagc aacttaaaac ccagcttaac tcaaaaattt
     3301 caggaatatc gctttgtttg taaaaaaggc cttcttctgg attcaagcct aaagcaagcc
     3361 aagtggcggc cactttataa
//
LOCUS       DAOOBB010000200         3337 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_31730_length_3337_cov_2.805911, whole genome shotgun sequence.
ACCESSION   DAOOBB010000200 DAOOBB010000000
VERSION     DAOOBB010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31730_length_3337_cov_2.805911"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..754)
                     /gene="pyrE"
                     /locus_tag="PLT31_08225"
     CDS             complement(83..754)
                     /gene="pyrE"
                     /locus_tag="PLT31_08225"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HPW95153.1"
                     /translation="MLNKDEFVHFLADTGALRFGDFITKSGRKTPYFINTGEFRTGQS
                     LSTLASFYADAFVEHFKNKAENLFGPAYKGIPLCAATAMKLSDAHNINLSFTYNRKEA
                     KDHGEGGTLVGYTYKEKTNVVIIEDVITAGTSVNETMETLKCIPNVNVVGLLISVDRR
                     ERLENGLSAMQIVSEKYNIETHSIININDIIEFLTKEENRKKINAPEDILERVHNYRN
                     EWGVA"
     gene            complement(771..>1300)
                     /locus_tag="PLT31_08230"
     CDS             complement(771..>1300)
                     /locus_tag="PLT31_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95154.1"
                     /translation="CFRWKNFYPLGESMTMHDDITSSENSESIDTNPIQETPVIEEAE
                     KATPSAPEVKEFQPTAPQVIVEKEKGFAHYVGFAFLTVLGITFFFMPGISIVYLIHQI
                     NPVNAPTAWIFAAVLSIILWILFKFKISGLVKATYFYLCFCLIIFAVLIVIYFTSQTG
                     NIFGNILAMLMGANP"
     assembly_gap    1301..1400
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1401..1705)
                     /locus_tag="PLT31_08235"
     CDS             complement(<1401..1705)
                     /locus_tag="PLT31_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HPW95155.1"
                     /translation="MNLTKYILQIHCPDQKGLIAGTTQVLAKAGANIIDMTQHTAKDI
                     ETFFLRAVFEIEEESIVEIERHVQTLEKHLQLNWKLFNTTKTPRIAIFVSKTDHCLY"
     gene            1793..3289
                     /locus_tag="PLT31_08240"
     CDS             1793..3289
                     /locus_tag="PLT31_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95156.1"
                     /translation="MHFSFYVFPFLLTIGLSSLWASPYLHVTTKLNLGFSGGPSASVF
                     ASDTVDRYGSNWRGLEIPLENGLYYERIDPFFALIFDAGLDSFRVHIEAPLRKDLEAW
                     YDSPLHSNWTLSPSDLDINVPTDAYAKWNNKAGFVQVGRFKPDLGPSPNTLAVGGAPY
                     HDAILWRFFASRFRYDFLLSSLNPWLHGTPESTGVMPGDSTEAYKQIYGSVDNQRNRS
                     YSEPYKTLIYHSFGLDFERFWVKFIEQSLVGGKQIEFRSMNPFMFLHDNYGSGYTKAS
                     SLFEWGFRPVPQSSFYWQVNLEDVASPVGETEGTTTRTIVSYFTGFRYEWKTLSQGDF
                     IFRFDVVYTDPAYNNQELPLLKYTSRRMYRSNYRSQTDPDFADMYFVDYPLGYRRGPD
                     ALDMWLDVSWKKGIHKANLELGYLRQGDKNLYTDYETAIQTSNGLSGVVESQYIADLI
                     YYRECVRFVKLYLGGGARFVQNFEHQEGEKEKDFWLRSGIVISNTFFD"
BASE COUNT          948 a          575 c          668 g         1046 t
ORIGIN      
        1 gaaagattct caggatgaaa aggctcaatg acaatatcgc cacattctac ggcttgagta
       61 atggcttttc ttgttaaaaa cattaggcaa ccccccattc attacggtag ttgtgtacac
      121 gctctaaaat atcttctgga gcattgattt ttttacgatt ttcttctttt gtgaggaact
      181 caataatatc gttaatatta ataatcgaat gcgtttcgat attatacttt tcgctcacga
      241 tctgcatagc ggaaagccca ttttcaaggc gttcgcgtcg atccacagaa attaaaaggc
      301 ccacgacatt cacgttagga atacatttta atgtttccat cgtttcattt acagaagtcc
      361 cagcggtaat cacgtcttca atgatgacta catttgtttt ttctttataa gtataaccta
      421 ccagagtacc gccttctcca tgatctttcg cttctttgcg attgtaagta aagcttagat
      481 tgatgttatg agcatcggaa agcttcatcg cggtagcagc gcaaagaggg attcccttat
      541 aagctggccc aaataaattt tcagctttat tcttaaaatg ttcaacaaag gcatcggcgt
      601 aaaaagaagc taaggtagaa agcgattgac ctgtacggaa ttcacctgta ttaatgaaat
      661 aaggcgtttt tcttccactt ttagtaataa aatcaccaaa acgtagagca ccggtatcag
      721 ctaaaaaatg gacaaattca tctttattta acatgtatcc tctatgttac ttaagggtta
      781 gcccccataa gcatggctaa gatattccca aagatatttc ctgtttgact agtaaagtaa
      841 attactatta aaacagcaaa aatgattaag caaaaacaca aataaaagta agtggctttc
      901 acaaggccag aaattttgaa tttaaacaaa atccagagga taatgcttaa tacagcggcg
      961 aaaatccaag ctgtaggtgc attcactgga ttaatctggt gaatgagata gacaatggat
     1021 attccaggca taaagaaaaa cgtaatgcca agaacggtta aaaaagcaaa accaacatag
     1081 tgggcaaagc ctttctcttt ttcgacaatc acttgagggg ctgtgggttg aaattctttg
     1141 acttctggag cagaaggtgt ggccttttca gcttcttcaa taacgggggt ttcttgaata
     1201 ggatttgtat ctatagattc cgaattttcg gaagaggtga tgtcatcatg catagtcatg
     1261 gattctccta aaggataaaa gttcttccag cgaaaacaaa nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn tcataaaggc agtggtctgt tttgcttaca aaaatcgcaa
     1441 ttcttggcgt ttttgtggtg ttgaaaagtt tccaattgag ttgaagatgc ttttctaaag
     1501 tttgcacgtg gcgttctatt tcaacaatac tttcttcttc aatttcaaac acggctctta
     1561 aaaaaaaggt ttcaatatct tttgccgtat gttgagtcat gtcaataata ttcgcgcctg
     1621 ccttggcgag cacttgtgtt gttccggcaa taagcccttt ttggtcagga cagtgtattt
     1681 ggagtatata tttagtcaaa ttcatgaggt aaatttaata atagaacaga ttcacgaact
     1741 gaaaaaaacg aaaacagaag ggaaaaagag tattaatttg taaattaata ttatgcactt
     1801 ctcattttac gtttttccct ttctcttgac tattgggtta tcctctcttt gggcgtctcc
     1861 ttatttgcat gtgaccacga agcttaatct aggcttttct ggaggccctt cggcgtctgt
     1921 tttcgcctca gacacagtgg atcgctacgg ttcgaactgg agaggcttag agattccact
     1981 agaaaatggg ctctattatg agcgaatcga tccctttttt gctttgattt ttgatgctgg
     2041 tttagattct tttcgtgttc atatagaggc tccactgcga aaagatttag aggcttggta
     2101 tgatagtcct cttcattcga actggacgct atctccaagt gatctagata tcaatgtgcc
     2161 tacagatgcg tatgctaaat ggaataataa agcaggcttt gttcaggtgg gtcgtttcaa
     2221 gcccgattta ggcccttctc ctaatacttt agctgttggt ggagcacctt accatgacgc
     2281 cattttgtgg cgatttttcg caagccgatt ccgttatgat tttcttttat cctccttaaa
     2341 tccttggtta catgggactc ctgagtctac aggggtaatg ccaggagact ctacagaagc
     2401 ttataagcag atttatggaa gtgtggataa tcaaagaaat cgttcttata gtgaacccta
     2461 caaaacatta atttaccata gttttggcct tgattttgaa cgtttttggg tcaagtttat
     2521 tgaacagagc cttgtgggtg gaaagcaaat agaatttaga tcgatgaacc cctttatgtt
     2581 cttacatgat aattatggaa gtggttatac taaggcaagt agtctttttg aatggggctt
     2641 taggccagta cctcaaagtt ctttttattg gcaagtgaat ttagaagatg tggcaagccc
     2701 agtaggtgaa acagaaggca ctacgacaag gacaattgtc agttatttta caggctttcg
     2761 gtacgaatgg aaaacgttaa gtcagggtga ttttattttc cgttttgatg tggtttatac
     2821 tgaccccgct tataataacc aggaacttcc tctgttgaag tatacgagcc gtcgtatgta
     2881 tcgtagtaac tatagaagtc aaacggatcc agactttgcc gacatgtact ttgtcgatta
     2941 tcctttaggc tataggcgtg gcccagatgc tttagatatg tggctcgatg tctcatggaa
     3001 aaaaggaatt cataaggcca atttagaact aggttactta cgccaagggg ataaaaatct
     3061 ctacacagat tatgaaacgg cgattcaaac cagtaatggt ctttcgggcg tggttgaatc
     3121 tcaatatatt gccgatttaa tttattatag agagtgtgtc cgttttgtca aattgtattt
     3181 gggcggaggt gcccgttttg ttcagaattt tgagcatcaa gaaggcgaaa aagaaaagga
     3241 tttctggctt agatcaggaa ttgtgatttc taatactttt tttgactgat ttgatcaaaa
     3301 aaatagattg gacgatggtg ttttttttct attttag
//
LOCUS       DAOOBB010000201         3290 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_32303_length_3290_cov_4.339722, whole genome shotgun sequence.
ACCESSION   DAOOBB010000201 DAOOBB010000000
VERSION     DAOOBB010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32303_length_3290_cov_4.339722"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1342)
                     /locus_tag="PLT31_08245"
     CDS             complement(<1..1342)
                     /locus_tag="PLT31_08245"
                     /inference="COORDINATES: protein motif:HMM:NF036484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HPW95157.1"
                     /translation="MDGMISLRGARLHNLKSIDADFQINAINVVCGPSGCGKSTLVLD
                     TLHGESRRRYLETLSPFALRVLGGKTFIPLDSASGLSPSIAIGASRGEAPPKSFAYTI
                     ANLDDSLHRLWSYAAQPICPICKKPMASSSREEIVSQIAALPISSRLQFLAPIETQEK
                     TLDSLAAVFLSQGYPKAMADGAPVSLSDLSSEQKKIIPKTFQLIIDRVIVRENSRTRI
                     AEAVDACFRFTHSFLELESGGTHTFYSSIPRCSEHGTTCPPLEASLFSPYKEESSSKT
                     LMTSTLKSHSWKEILETSFEKLQTLLPALFEGILPEYLILTAETLYQKINAINRLGLG
                     YLSCGREGSSLSSGELQRLRLSPIATGYLNGMLICLDEPASGLHSNDVEKLWSILLEI
                     KNQGNTLILIEHHPEIISKADRIIEMGPLAGSRGGEILFQGSRDEVLNNPLSPTG"
     gene            complement(1330..1902)
                     /locus_tag="PLT31_08250"
     CDS             complement(1330..1902)
                     /locus_tag="PLT31_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95158.1"
                     /translation="MINRIIFFFLICGLCFQTQATEKDSPSESLKAFIGLSGGNSMIA
                     RDGTLWMSIKGGVRVHPRIQTGLFVSTIVSDVKNPYKKATSQYIDYNALGAFAEFRLL
                     EMNLFSLSIPFSVGGGLINIMEQNVEHSSAEDGFFMGEAGLSFNYQLTRVLNVSIGGG
                     YRLFLHVDNNKLDNNDFNSLYGELTFRWTE"
     gene            complement(1899..2855)
                     /locus_tag="PLT31_08255"
     CDS             complement(1899..2855)
                     /locus_tag="PLT31_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HPW95159.1"
                     /translation="MKSFFPKKSFEENVDYYAARCPAFVRIIIISTKSFLYYHGMMRA
                     AAMTYNTFLAAIPLLILLTAIALAIGLGNFFTDYLPYFNKLFSWNVPMEELMPLLANA
                     EHIPLAKLGFVGSAALFITFLLTMGSLESNFNVIWENKTSRKILHQILVYSPILLVFG
                     GFIGIFAGVVSQIQNTLSLIVIEGLHFTENDLGILINAFWWIALNFGVVLVIFLMLYA
                     LPSRPTKIPRKKLLLTSIFASICSWIAIYIYSLILVLVQSTMIVRMSLFYGSLGFIPL
                     ILLFVFGVWSIILFANSLVWTICTWPESSHKTWNWIGTSVNQ"
BASE COUNT         1138 a          565 c          642 g          945 t
ORIGIN      
        1 ggcctgtagg tgaaagaggg ttgtttagca cttcatcgcg agaaccctgg aataaaatct
       61 ctcctccacg agaaccagcg agaggcccca tttctatgat acggtctgct ttagatatga
      121 tctctggatg gtgttcgata agaattaacg tattcccttg gtttttaatt tctaacaaaa
      181 tagaccataa tttttctaca tcattagaat gtaaaccgct agcaggttca tcaaggcaaa
      241 tcagcatccc gttcaggtaa ccagtcgcta ttggagaaag ccgtaaccgt tgtaactctc
      301 cacttgataa agaagaacct tctctaccgc aagaaagata acctaagcct aagcgattga
      361 ttgcgtttat tttttgatat aaggtttctg cagtgagtat gagatattct ggaagaatgc
      421 cttcaaataa agctggtaac agagtttgta acttttcaaa agaggtttct aaaatttctt
      481 tccaagagtg agattttagt gtggaggtca ttaacgtttt agaactgctt tcttctttat
      541 aaggagaaaa aagcgacgct tcaagaggtg ggcaagtcgt tccgtgttca ctgcatcttg
      601 gaatggaact ataaaaagta tgagttccac cagattcaag ttctaaaaaa gaatgagtaa
      661 atctaaagca ggcatctacg gcttctgcaa ttcgcgtacg actattttct cgaacaatta
      721 ctcgatcgat aattagttga aatgttttag gaattatttt tttttgttca gaagataaat
      781 cgctgagtga aacaggcgcg ccgtcggcca tggcttttgg atatccttga gatagaaaaa
      841 cggcggctaa agaatcaaga gttttttctt gtgtttcaat aggagctaaa aattgaagtc
      901 ttgaagaaat ggggagtgct gcaatttgag aaacgatttc ttcacgagag gaagatgcca
      961 tcggcttttt acaaatgggg caaatcggtt gagctgcata agaccaaaga cggtgaagag
     1021 agtcatctag gtttgcaatg gtataggcaa aagattttgg cggtgcttca ccgcgagatg
     1081 ctccaatcgc aatacttggg gaaagccctg aagcactatc cagaggaata aacgtttttc
     1141 cacctaaaac gcgcagagca aaagggctga gtgtttcaag atagcgtctc cgactttctc
     1201 catgtagagt gtcaagaact aacgttgatt taccgcaacc agaagggccg caaacgacat
     1261 tgatcgcatt gatctgaaaa tcagcgtcaa tggattttaa gttatgtaat ctcgctccgc
     1321 gaagcgaaat cattccgtcc atctgaatgt aagttctcca tagagtgaat taaaatcatt
     1381 gttatccagt ttattattgt ctacgtgtaa aaataaacga tagccaccac caatagaaac
     1441 attgaggacg cgtgttaatt gatagttaaa tgaaagccct gcttcaccca taaagaaacc
     1501 atcttctgca cttgaatgct ccacattttg ttccatgatg ttgattaaac caccaccgac
     1561 agaaaaagga atagatagag aaaatagatt catctcaagt aagcgaaatt cagcaaaggc
     1621 tccaagagca ttataatcaa tgtattgaga tgttgctttt ttataagggt ttttaacatc
     1681 gcttacgatg gtggaaacaa acaagcctgt ttgtattcga ggatgcactc gaacaccgcc
     1741 tttgatactc atccagagag tgccatctct tgcaatcatg gaattgccgc cacttaaacc
     1801 tataaatgct tttagggatt cagaaggaga atccttttct gtggcttgcg tttgaaaaca
     1861 taaaccacat ataagaaaaa agaatattat acgattgatc attggtttac cgatgttcct
     1921 atccagttcc atgttttatg acttgattca ggccaagtac aaatagtcca aaccaagctg
     1981 tttgcaaaca atataataga ccatacacca aaaacaaata gcaaaatcaa tggaataaaa
     2041 cctaaagatc cataaaagag cgacattcta acaatcatcg tggattgaac taaaacgaga
     2101 atcaaactat agatataaat agcaatccag gagcaaatag aggcaaagat ggatgttaat
     2161 agcaactttt tccttggaat ttttgtgggg cgagagggta aagcatagag catcagaaaa
     2221 ataacgagaa cgactccaaa attgagtgcg atccaccaaa aagcgttaat tagaatacct
     2281 aaatcatttt cggtaaagtg aagaccctct atgacaatca gagaaagagt gttttgaatt
     2341 tgagaaacga cacctgcaaa aattccaata aatcccccaa aaacaagaag tattggactg
     2401 tagactaaaa tttgatgaag aattttcctt gatgttttat tttcccaaat cacattgaaa
     2461 ttagactcaa ggctccccat tgtcagtaaa aaagtgataa aaagagctgc tgaaccgaca
     2521 aatcctagct ttgctaaagg aatatgctct gcattggcaa gtagaggcat taattcttcc
     2581 attggaacgt tccaggagaa aagcttatta aaatacggta ggtaatcagt aaaaaaattc
     2641 ccaagaccaa ttgcaagggc tatggcggtt aacaaaatta aaagaggaat ggctgctaaa
     2701 aaagtattgt acgtcatagc agcggcacgc atcataccat gatagtaaag aaaggacttc
     2761 gtcgagataa taatgattct tacaaatgca gggcatctcg ctgcgtaata atccacattt
     2821 tcttcaaaac tttttttagg aaaaaaagat ttcatcgatt aaaattaatc attttcctct
     2881 aaaaaaaaga aaaagaatgt aaatcatgtt gttttaaata tagggttaaa gttcctttag
     2941 tagggaaaag gctttatcat ttgtaaaaag tcgttttctg ggcaaggata ataaaaagag
     3001 aacaagaatt ttatcttggt ttttacgcga aaaaagccgt ttaatgcaaa atgaagggtt
     3061 ttaataaggt attcctgttg agaactagat aaatctttag atgcatttat tcaattcaaa
     3121 aaaagttgta aatattttat tacaagtgac tttgatgtgc tcatagaatt taaaatgcct
     3181 tttttttgat aaaatatgaa agtgtagaca ttaataatga tttgtatttg tttaaaacgt
     3241 tatttcgttt ctaaaacaca taaaaagtgt agactttcaa tttataatta
//
LOCUS       DAOOBB010000202         3268 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_32608_length_3268_cov_3.602863, whole genome shotgun sequence.
ACCESSION   DAOOBB010000202 DAOOBB010000000
VERSION     DAOOBB010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32608_length_3268_cov_3.602863"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(279..1238)
                     /locus_tag="PLT31_08260"
     CDS             complement(279..1238)
                     /locus_tag="PLT31_08260"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPW95160.1"
                     /translation="MNISLKLILGLACSFSLLTAAEQVPASIDVVDDAAPAVSSTSSS
                     TEKTVAPAAPVTQKEPSAMVAQEPPAQISGAPAPTQNTSSQKRMVVQPKPEPIVIRVP
                     ARQTNRQPIVIENDAPSRTDVKALRNYYPIELKLAISASLGSAMFLNSDVDMPDFSGL
                     FWNAGLIVIFPLNESTVGMTAGALFKMRNVSAVISEESMDNRYRFSQMSVDVPLFFKF
                     KGARSRLAFDTGVQMSVNLYDKLEVSTGPSDKEYDLMDASLRTPIDWSLVGGFSVIIN
                     TRIAFNMKFLIGLSDMYSTSEVENISSDFSPVEASMGLIVNIL"
     gene            complement(1295..2452)
                     /locus_tag="PLT31_08265"
     CDS             complement(1295..2452)
                     /locus_tag="PLT31_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HPW95161.1"
                     /translation="MLYQEALQEFLPSTLKSLSTEALAALDYQEECEIKQKAFNRFWS
                     SNRLDGTPLKIIESPLSRNYRTTSKRRVTVDRCRVIFEGSGSTSFVLEPQEHEQIFSY
                     LNTLLAKPAFLPLAYAMNWIIIRGSYTSRVVIFNVFKMDASIVRKMKQVVTHLQSMNV
                     TVHGAHLYYDPTRSDYYLESFRPSDSVSLKHLFGLRDLTLHIEDIKLKYPVTGFSQIN
                     ESQIPNLISTANDFAKISKDDTFLDLFCGYGLFSLTVGKNAKSVIGADWEGASIEYAK
                     ASARFLNRKEVRFSAGKITSEWIESHLPPMKNESEIQLLDPPRQGTDPGVISSLAKRN
                     PKRVVHVFCGTDVIPKELTVWNSKGYKIKKIQPLDLFPGTSHIETMVLLEK"
     gene            complement(2689..>3268)
                     /locus_tag="PLT31_08270"
     CDS             complement(2689..>3268)
                     /locus_tag="PLT31_08270"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HPW95162.1"
                     /translation="HDHKVSIWDEWADEKGNLGPVYGHQWRHWPTPNGGEVDQIQVLI
                     DSLKNNPDSRRHLVCAWNVGQIDQMALPPCHCLFQFYVAEGKLSCQLYQRSADCFLGV
                     PFNIASYALFTMMLAQVTGLKLGEFVHTFGDLHLYLNHINQAKEQLSRTPRALPTMKI
                     NPDVKDLFSFKLEDFELVDYDPYPTIKAPIAV"
BASE COUNT         1094 a          613 c          580 g          981 t
ORIGIN      
        1 tgacaatttg aaccttcact atttgattag ctaatcaaat aaacgttcgg ttcctcatga
       61 aaaaactcgg ctattttaga tgaaattctc attgtttcgg catgaaaaaa cgggcttatt
      121 tagaggggcg tctcattttc atacaagcat tgctgtgccc ttgtaaaagg tacaagtatt
      181 tgtattggaa ccattttttg atatgttttt tacgttacct tagacaataa aaaaaccgct
      241 gattttattt cagcggctgt tttcataatc aagaaagatt acagaatatt cacaataaga
      301 cccatagaag cttctacagg actgaaatca gaacttatgt tttccacttc tgatgtacta
      361 tacatatctg ataatccaat taagaatttc atattaaaag caattctcgt gttaattata
      421 acagaaaaac caccgactaa actccaatca ataggagttc taagagaagc atccatcaaa
      481 tcatattctt tatcagaagg acctgtcgaa acttcaagtt tatcatacaa atttacagac
      541 atctgaacac ctgtatcaaa agcaagacga gatctagcac ccttaaactt aaagaataaa
      601 ggaacatcta cactcatctg agaaaaacga taacggttat ccattgactc ttcagatata
      661 acagcagaaa catttctcat tttaaataaa gcacctgcag tcattccaac agtcgattcg
      721 tttaaaggga aaatgacaat aagacctgca ttccagaata aaccagaaaa atcaggcatg
      781 tctacatcag aattcaagaa cattgcactt cctaaagaag cactaatcgc taatttcaat
      841 tcaattggat aataatttct caatgcttta acatccgttc ttgatggagc atcgttttct
      901 atgacaatcg gttgacgatt agtttgtcta gcaggaactc ttatcacaat tggttctggc
      961 ttaggttgaa ccaccatacg tttttgagaa gacgtgtttt gtgttggggc tggagcaccg
     1021 ctgatttgtg caggaggttc ttgagccacc attgcagaag gctctttttg agtgacggga
     1081 gcagcaggag ccactgtttt ctctgtactt gaagaagtag aagaaacagc aggagcagca
     1141 tcatcaacga catcgataga tgcaggtact tgctctgcag ctgtcaataa tgaaaatgaa
     1201 cacgctaaac ccaaaatcaa ttttaatgaa atattcacat tagcctccaa ataaatttgt
     1261 aaataaaatt agataaaaaa gattagatat gcacttattt ttctaacaaa accattgttt
     1321 ctatatgaga tgtacctgga aataaatcta atggttgtat ttttttgatt ttatagcctt
     1381 ttgaattcca tacggttaat tcttttggaa tcacatctgt accgcagaaa acatgaacca
     1441 ctcgcttagg attccttttt gccaaagaag aaatgacccc tggatccgtt ccttgacgtg
     1501 gtggatcaag caattgaatt tctgattcat ttttcattgg cggtaaatga gattcaatcc
     1561 attcagaagt aatttttcca gcactaaaac gtacttcttt tctatttagg aaacgagcac
     1621 ttgctttagc atattcaata gaagcccctt cccaatcagc tccaatcaca gactttgcat
     1681 ttttacctac cgtcaaagag aataagccat aaccacagaa aaggtctaag aaggtatcat
     1741 cttttgatat ttttgcaaaa tcattggccg tagaaatcaa atttggaatt tgagactcat
     1801 tgatttgact aaatccagtt acagggtatt ttaacttgat atcctctata tgaagagtta
     1861 aatcacgcag gccaaataag tgttttaatg aaacagaatc ggaaggacga aatgactcta
     1921 aatagtagtc agaacgagtc gggtcataat aaagatgagc tccatggaca gtaacgttca
     1981 tggattgtaa atgggtaacc acttgcttca ttttacgaac aatagaagca tccatcttaa
     2041 acacgttgaa tattaccact ctagaagtat aggaaccacg aataataatc cagttcatcg
     2101 cataagctaa aggtaaaaat gcaggtttag caagtagcgt atttaagtac gaaaaaattt
     2161 gttcatgttc ttgtggttca agtacaaaag aagtggagcc actcccctca aaaatcacac
     2221 ggcaacgatc tactgtgact cgtcgtttgg aagtggttct ataatttctg gataatgggc
     2281 tttcgattat ttttaagggc gtcccatcga gtcgattaga actccaaaaa cgattaaagg
     2341 ctttttgttt aatttcacat tcttcttgat aatcaagagc cgctaatgct tctgttgata
     2401 gactttttaa tgtggaggga aggaattcct gaagagcttc ttgatataac atagttgcaa
     2461 atataaaaga tttattgttt taagggaatc ccctaagtcg atttgagcct gatgaaatca
     2521 agagaatggg ccttatattg acttttcaag aaatgatgag aaagatttaa ctaaaaggaa
     2581 ttcttatgaa acaagtaaat caataaatac ctgtttgaac ttattgaata caacaaagaa
     2641 tcatccctct ttattttagg caatacgcct tatcaataac acaaataatt aaacagcaat
     2701 aggtgcttta attgttggat acggatcgta gtctaccaat tcaaaatctt ctagtttgaa
     2761 agaaaataag tctttcacat ctggattgat tttcatggta ggcaaagccc gaggagtacg
     2821 agatagttgt tctttcgctt gattaatgtg attgagatac aaatgtaaat caccaaaggt
     2881 gtgaacgaat tcccctagtt ttaatccagt cacttgagca agcatcattg taaaaagagc
     2941 gtatgaggca atattaaaag gaacgcctaa aaagcagtct gcactacgtt ggtataactg
     3001 gcaagacaac ttcccttctg ctacataaaa ctggaaaaga cagtggcatg gaggcaaagc
     3061 catttggtct atctgaccca cgttccaagc acataccaaa tgtcttctgg aatcagggtt
     3121 attctttaaa ctatcgatca aaacctgaat ctgatccact tcgccgccat taggtgtcgg
     3181 ccaatgcctc cactggtggc cataaacagg gccaagattc cctttctcat ccgcccattc
     3241 gtcccaaatg gagactttat gatcatgt
//
LOCUS       DAOOBB010000203         3251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_32813_length_3251_cov_4.870150, whole genome shotgun sequence.
ACCESSION   DAOOBB010000203 DAOOBB010000000
VERSION     DAOOBB010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32813_length_3251_cov_4.870150"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1468
                     /gene="nadE"
                     /locus_tag="PLT31_08275"
     CDS             <1..1468
                     /gene="nadE"
                     /locus_tag="PLT31_08275"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HPW95163.1"
                     /translation="VIKNFLPKTLLPCYREFDEPRYFSDLRRLAFSLNQPLEALLKPY
                     TRKDGIQIGISICEDAWDSIYEIKPVSILASQMNENGFLINISCSPFTLGKTDARHRV
                     FAAHASQNKIPLLYLNSVGTQNNGKNIYAFEGDSCFYRATGEKAFEYPKFESYSTVIE
                     LDGKEIKVSEKSKPQLTGMAEVRKALVYMLSENLKRWGIERVVIGASGGIDSAVSAVL
                     YTEALGADRVFLVNMPTRFNSKTTRNAAKDLADNLGCPYLEAPITDTIDVFCRGLSSL
                     SFERSSASLKVEGIHYENLQARTRSATFLATIASVLNAAFTCNGNKSEVMTGYCTLYG
                     DTCGIVCALGDLWKTQVYELAREMNKEREIIPETSILIPASAELSDAQNVDEGKGDPI
                     VYAYHDKLFSFWMERWNRNSLEDSIEALKDGSLYEKLSISKDFFDSLFKSYEDAIADM
                     KYWWGRYKGIALAKRIQMPPVLCVSRRAFGFDLRESQI"
     gene            complement(2255..3124)
                     /locus_tag="PLT31_08280"
     CDS             complement(2255..3124)
                     /locus_tag="PLT31_08280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HPW95164.1"
                     /translation="MKYFIVDVFSKNKYSGNQLAVVFLSSSLSEMEMQTIANEFHFSE
                     TTFVYPQKNDDGSYRVRIFTPNNEVPFAGHPVLGTAYIIRKELEDNNPHKINLSLNIG
                     IISVTFDDTEKIQWMKQIEPTFGSKYKAKDIAEIIGVTEEEIDIHFPIQEVSTGIHFL
                     LVPLTGLNAIKKSRINLSKYKEFFKGKSETPIFIYCKETYEHENEFNCRMFADALGVP
                     EDPATGSANGCFAAYLSKYEILGTKKVNVKVEQGYEINRKSILHLRTQNESNKYNIEV
                     GGKVIKVAEGHLE"
BASE COUNT          855 a          547 c          687 g         1162 t
ORIGIN      
        1 agtgatcaag aattttttgc cgaagacgct tttgccttgt tatcgagagt ttgatgaacc
       61 tcgttatttt tctgatctgc gtcgtttggc tttttctttg aatcaaccgc ttgaggcttt
      121 attaaagcca tatactcgaa aagatggtat tcaaattgga atctctatat gtgaagatgc
      181 ttgggattcg atttatgaga ttaagcccgt ttctatttta gcttctcaga tgaatgagaa
      241 tggcttcttg attaatattt cttgttcgcc ttttacgctt gggaaaacgg atgcacgtca
      301 tcgtgtgttt gctgctcatg cctcacaaaa taagattcct ttactttact tgaattctgt
      361 gggcactcaa aataacggta aaaatattta tgcctttgaa ggcgatagtt gtttttatag
      421 ggcgactggg gaaaaggctt ttgaataccc taaatttgaa tcttatagta cagttattga
      481 attggatggt aaagagatta aggtttctga gaagtctaag cctcaattga cagggatggc
      541 agaagttcgt aaagcccttg tttatatgtt atcagaaaat ttaaaacgct ggggcattga
      601 acgagtggtg attggggctt ctggcggaat tgatagtgcg gtctctgcgg tgctttatac
      661 agaggcactt ggagcggatc gcgtttttct tgtgaatatg cctacgcgat ttaattcgaa
      721 aacgacgcgt aatgcagcaa aagatttagc tgataattta gggtgtcctt atttagaggc
      781 tcctattacc gacacgatcg atgtattttg tcgagggctc tcttctcttt cttttgaaag
      841 aagttctgct tctttgaaag tagaagggat tcattatgaa aatttacagg cgaggactcg
      901 ctcggccaca tttttagcga caatcgcttc tgttttaaac gccgctttta catgcaatgg
      961 gaataaaagt gaggtgatga caggttattg tacgttatac ggagatacct gtggtattgt
     1021 ttgtgcttta ggcgatctgt ggaaaactca agtgtatgaa ctcgcgcgag agatgaataa
     1081 agaaagagag attattccag aaacctcgat tttaattcct gcaagtgccg aattgagtga
     1141 tgctcaaaat gtagatgaag ggaaagggga tcccattgtt tatgcttatc atgataagct
     1201 gtttagtttt tggatggaac gatggaatcg gaattcttta gaagattcta tcgaggcatt
     1261 gaaagatggg tcattatatg agaagctatc aatatcaaaa gatttctttg attcgttgtt
     1321 taagagttat gaagatgcga tagcggatat gaagtattgg tgggggcgtt ataagggaat
     1381 cgcgcttgcg aaaaggatac agatgccgcc tgtgttgtgt gtgtctcgcc gagcctttgg
     1441 ctttgactta agggaatcgc aaatataaac gtcgcgaaaa tggcgaatat aaacttcgta
     1501 gtacttcgtt attacttgtt cagcgtacta gttgtacgcc ttcagtcgta atgccttgtt
     1561 ctactcgttt ctcttcgcca ttttgggaaa tattttttct gaagtatggc aaaacttcgt
     1621 tgcataaatg atttgcaatc gatcgaaaag ccaggttgtg ctggctttaa ttttcaattc
     1681 agtttttttt tacgcatttt gcgattcagg tgtaatataa acgtcgtagt acggtcgtta
     1741 ttacttgttc agcgtactat ttgtacgcct tcagtcgtca tgccttgtcc tacttgcttc
     1801 tcttcgccat tttgggaaat attttttttg aagtatggca aaacttcgtt gcataaatga
     1861 tttgcaatcg atcgaaaagc taggttgttc tggctttaat tttcaattca gttatttttt
     1921 tacgcatttt gcgattcaag tgtaatataa actcgtagta cttcgttatt acttgttcag
     1981 cgtactagtt gtacgccttc agtcgtaatg ccttgttcta ctcgtttctc ttcgccattt
     2041 tgggaaatat tttttctgaa gtatggcaaa acttcgttgc ataaatgatt tgcaatcgat
     2101 cgaaaagcca ggttgtgctg gctttaattt tcaattcagt ttttttttac gcattttgcg
     2161 attcaggtgt aatataaact tcgtagtact tcgttattac ttgttcagcg tacatttgta
     2221 cgccttcagt cgtcatgcct tgtcctactt gcttctattc taaatggcct tcggctactt
     2281 tgatgacttt tccgccgact tctatgttgt atttatttga ctcgttttgt gttcgaagat
     2341 gaaggattga tttccgattt atttcatatc cttgttctac ttttacattc actttttttg
     2401 tcccaaggat ttcatatttt gataggtagg cggcaaagca accatttgcg cttccagtag
     2461 caggatcttc tgggacaccc aatgcgtctg caaacattct acagttgaac tcgttttcat
     2521 gttcatacgt ttctttgcag tatataaaaa tgggcgtttc actctttcct ttgaaaaact
     2581 ctttgtactt actcaggtta atgcgtgatt tttttatggc gtttaatcct gttagtggaa
     2641 ctaagagaaa atggattccc gtcgatactt cttgaatggg aaaatggatg tctatttctt
     2701 cttcggtgac gccgatgatt tcagcgatgt ctttggcttt gtattttgaa ccaaaggtgg
     2761 gttcgatttg tttcatccac tggatctttt ctgtgtcatc aaatgtcaca gaaataatac
     2821 caatgtttag gcttaagttt attttatgag gattattgtc ttctaattct tttcgtatga
     2881 tgtacgccgt acctaatacg ggatggcctg caaagggaac ttcattattt ggagtaaata
     2941 ttcttactcg ataagagcca tcgtcatttt tttggggata aacaaatgtg gtttcagaaa
     3001 aatgaaattc attggcgatg gtttgcattt ccatttctga taaactagat gacaagaaga
     3061 caacggcaag ctggttccct gagtatttat ttttagaaaa aacgtctacg atgaaatatt
     3121 tcattttatt aataagaatt agctgtgaaa aaccatattc aaatctaacc tgaattcaag
     3181 tcactaacgc aatctgatta gattaaaaag gtctagccga aatgctacct tttaaattac
     3241 taaacataag a
//
LOCUS       DAOOBB010000204         3251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_32814_length_3251_cov_4.494368, whole genome shotgun sequence.
ACCESSION   DAOOBB010000204 DAOOBB010000000
VERSION     DAOOBB010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32814_length_3251_cov_4.494368"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..>3251
                     /locus_tag="PLT31_08285"
     CDS             46..>3251
                     /locus_tag="PLT31_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPW95165.1"
                     /translation="MSFDFSFAQLNEWNGNPTVFGVNVLKPHATSMPYSSIEEALKGD
                     RRGSEWYQTLAGTWKFHHVNKPSERHTSFFEDSFDASSFDEIEVPSSWQLYGYDKPIY
                     TNVTYPWSGTDFINPPAAPTNFNPVGHYRRTFTVPANWDGKRIRLHFEGVESAFYVWI
                     NGKYVGYSENSFTDHEFDITDKLRAGTNNISVQVFRWCDGSWMEDQDYIRLAGIFRDV
                     YLYATPKAHIQDFQIDANLLSNYKDGDLKASVWVDNFTTAAVSNYSVELQLYADNGTE
                     VGPKMQKSISSIAPNGGEQKVQFQTTVMSPKLWSGEYPNLYTLVLVLKDGSGEVVQIE
                     SAKIGFRKVELKKDGTGATRYFINNKPIKFRGVNRHEMDPDHGRVMSYERMEEDVKLM
                     KRFNINALRMSHYPNDPRMYDLCDRYGIYVIDEANLETHGAWDKVPKSNNDWRAASVE
                     RMSSMIQRDKNHPSVVLWSLGNEAGNGDVFGSMREYAHSADSTRPVHYEGDWNNADVN
                     SWMYYGYDAVRTYNNNSKPIMLCEFEHAMGNSVGELKEYVDAFYANPRSFGGFIWDFI
                     DQGLRRDNTKFFNFGGLWGDRPNDDNFCANGLVFPDRTLQPEIWEVKHQYRNILVKDV
                     DVASGVVSIESRFDFTNIGDEVDLVWTLKEDGKAIETGTVSGTSLNISPLSKKEVVIP
                     FKQPKTKAGSDYYLNLDFRLKKTTFWADAGFSIAEEQFKLSLGEALPPQIDITSLPLQ
                     NVAQNNGKVTIEGANYSVGIDLATASITDYKINGVQIIKDGPTPNFWRAPTDNDKGNG
                     MENRCGSWKFAGRDRVINSSKVTTVSEKETRIDFEIGLPKAGSSKMTMSYTIYGSGDI
                     IVDYTFSPDGSLSEIPNVGTLFTVPGGFEKVTWYGRGPHENYVGRNQGSHMGIYSTVA
                     DSMTIPYMEIGETGQRTNVRWMTLTNSTGMGVMVVGSPFMEFSAQHYTPQQLTDVKLP
                     WDLKRDKDITLRVDYRQMGLGGINSWGAKPLDAYMNFSKNTYKHKFRISPLPGNLEDP
                     TILAGLGFKNLETSTEKIKYPDIEYTELKSEPLVAMSFP"
BASE COUNT         1024 a          509 c          718 g         1000 t
ORIGIN      
        1 gatatgactt taaaaaaact tacaacagct ttattcattc ttttaatgtc attcgatttt
       61 tcttttgctc aattaaatga atggaatgga aatccaacag tatttggcgt gaatgtatta
      121 aagccacacg ccacatcaat gccttatagt tctattgaag aggctttaaa aggggacaga
      181 cgaggttctg aatggtatca aacactcgcg ggtacatgga aatttcatca tgtgaataaa
      241 ccctctgaaa ggcatacgag cttttttgaa gattcttttg atgcttctag ttttgacgag
      301 atagaggttc caagttcttg gcaattgtat ggctacgata agccaattta tactaatgta
      361 acttatccat ggtctggaac ggattttatt aaccctccag cagctccaac aaattttaat
      421 cctgtagggc attatagaag aacttttact gtgcctgcaa attgggatgg taaacgtatt
      481 cgtttgcatt ttgaaggtgt ggaatctgca ttttatgtat ggattaatgg aaagtacgtc
      541 ggttatagtg aaaactcttt tacagaccac gaatttgata ttacagacaa attgagagca
      601 ggcactaata atatatcggt tcaagtattt cgctggtgtg atggtagctg gatggaagac
      661 caagactata ttcgtttagc tggtattttt agagatgtgt atctctacgc gactccaaaa
      721 gcacatattc aagattttca aattgatgcg aatctcttgt caaattataa ggatggagat
      781 ttaaaggctt ctgtttgggt tgataatttc acgacggctg cagtttcgaa ttattcagta
      841 gaacttcagc tttatgcaga caatggtacg gaagtagggc caaaaatgca aaaaagcatt
      901 tcttctattg ctccaaatgg aggagaacaa aaagtacagt tccaaacaac tgtgatgtct
      961 ccaaagcttt ggtctggaga gtatcctaat ttatatacgt tagttctagt tcttaaagat
     1021 ggtagtggtg aagtagtaca aatagaaagt gcaaagattg gatttagaaa agtagaattg
     1081 aaaaaagatg gtactggagc tacacgctat tttattaata ataaaccaat caaattcaga
     1141 ggggtgaatc gtcatgaaat ggatcctgat catggtcgag tcatgtctta tgaaagaatg
     1201 gaagaagacg tcaaattaat gaaacgattt aatatcaatg ctttgcgtat gtctcattat
     1261 ccaaatgatc caagaatgta tgatctttgt gaccgttatg gaatttatgt gattgatgaa
     1321 gccaacttag aaactcatgg tgcttgggat aaggttccta agagtaataa tgattggagg
     1381 gctgcttctg tagagcgtat gtcttctatg attcaaagag ataaaaatca cccttctgtt
     1441 gttctttggt ctttaggcaa tgaagctgga aatggagacg tttttggttc tatgagagaa
     1501 tatgctcata gtgcggattc tacacgccca gtgcattatg aaggcgattg gaataatgcg
     1561 gatgtgaata gttggatgta ttatggttat gatgccgttc gtacttataa taacaattca
     1621 aagcctatca tgctttgcga attcgaacat gccatgggaa atagtgttgg tgaattaaaa
     1681 gaatatgtag atgctttcta tgcaaatcct cgttcttttg gtggctttat atgggatttt
     1741 atcgaccaag gtcttcgtcg tgataatacc aagttcttta actttggtgg gttatgggga
     1801 gataggccaa atgatgataa tttctgtgct aatggccttg tgttcccaga ccgcacttta
     1861 caacctgaaa tatgggaagt gaaacaccaa taccgcaata ttcttgtaaa agatgtggat
     1921 gtggcaagtg gcgtggtttc cattgaaagc cgttttgatt ttacgaatat aggagatgaa
     1981 gtcgatttag tttggacatt aaaagaagat ggaaaggcaa tagaaacagg gactgtttcg
     2041 ggaacatcct taaatatttc gccattaagt aaaaaagaag tggtgattcc atttaagcaa
     2101 cccaaaacta aagcgggttc ggattattat ttgaatttag attttagact taagaaaacc
     2161 actttttggg cggatgctgg ttttagcatt gcagaagaac agttcaagtt gtctttagga
     2221 gaagccctcc ctccacaaat cgatattaca tctcttccgt tacaaaatgt cgcacaaaac
     2281 aatggaaagg tgacaattga aggggccaat tattctgtgg ggattgattt agctactgca
     2341 agcatcacag attataaaat caatggtgtt caaatcataa aagatggtcc tactccaaat
     2401 ttctggcgtg ctcctacaga taatgataaa ggaaatggaa tggaaaatcg ctgtggcagc
     2461 tggaaattcg cgggtcgtga tcgagtgatc aatagttcaa aagtgacaac cgtttcagaa
     2521 aaggaaactc gcattgactt tgaaattggg cttccaaagg caggtagttc aaaaatgact
     2581 atgagctata ctatttatgg tagtggtgat atcattgtag actatacttt ttctccagat
     2641 ggttctttat ctgaaattcc aaatgtggga acgcttttca ctgttccagg cggctttgaa
     2701 aaagttacat ggtatggaag aggtccgcat gaaaattatg tcggaagaaa tcaaggaagt
     2761 catatgggga tttattcaac ggttgccgat tccatgacga ttccttatat ggaaatagga
     2821 gaaacaggtc agaggactaa tgttagatgg atgacattaa ctaatagcac aggaatgggt
     2881 gtaatggtgg taggttctcc atttatggaa tttagtgctc aacattatac accgcaacag
     2941 ttaaccgatg tcaagcttcc atgggattta aagcgagata aagacattac tcttcgtgtg
     3001 gattatcgcc aaatgggttt aggtggaatt aatagctggg gcgcaaagcc attagatgct
     3061 tatatgaatt tttcaaaaaa tacttataag cataaattcc gtatatctcc tctccctgga
     3121 aaccttgaag atccaacgat tcttgcaggt cttggtttta agaatttaga gactagcact
     3181 gagaaaatta aatatcctga tattgaatat acagagctta agagtgaacc cttagtcgcg
     3241 atgagtttcc c
//
LOCUS       DAOOBB010000205         3226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_33160_length_3226_cov_3.340902, whole genome shotgun sequence.
ACCESSION   DAOOBB010000205 DAOOBB010000000
VERSION     DAOOBB010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33160_length_3226_cov_3.340902"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2235)
                     /locus_tag="PLT31_08290"
     CDS             complement(<1..2235)
                     /locus_tag="PLT31_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95166.1"
                     /translation="MKKETFLKSYGIPAGLAFLFLIILLVVFRDFAFDSSKLMVNSDQ
                     LNGIGSRVLRAENAIVTEWDDSRLGGVPTIDALFADVYHPLVWIQFLTDPARAVGFKF
                     ILTIWIAFGSAMLLGRALTQSWWLGGLLGFLYAFSPQYFTYVYGGHDGKMMVFAIAPL
                     AILSIRKIIREARLKYFIAFVLSVVWMILGSHLQLTYLFLWGAGLYALYEMFFLDAAR
                     SIKVKRIGMAAFGLAIALLISSFQIIPPYLYTTKQSVRGTDEKTNYGHAVSWSLHQEE
                     LASILLPGFIGIDVYEQDQKTGDLKGSSHVNISMKDYQNWGIQGSPFYWGHNHFKLNH
                     DSSGALLTFLAFLCFFIPNKKRFAIFWFLGATLALSYAMGAHSPLFKLWFEILPGVKN
                     FRAPSMALFWFPLVMVMMAAPVLKAFKDPESKKPFLNGLFLFALLTIITLIARYQWEL
                     FLGPVGLVVSILYGFIFITVINITDRKETLSFQNFISAIKNKWNGSSLIELGLILVPF
                     LLLGIFMLSGQNLLMDPVTAPYFKPLNTNLMNFTSAKVIPGFITVVFTMVAAWFILSQ
                     RFESYKKVLLLAILATIELYTINGVFVQNTEYNKYIQPNNPVIHAIKQNSENPSHPAR
                     VLSLSRSEALGGNAFPIYKMRNADGFHDNELASYRAFRGGQNNANYMIGISDPQAPKP
                     FLDLMNIGAVIFDTRQGTTYMPNPTAMGEAFLYGSSTVLDDAKAIDTLIAGFPYREQI
                     ILAEA"
     gene            complement(2791..>3226)
                     /locus_tag="PLT31_08295"
     CDS             complement(2791..>3226)
                     /locus_tag="PLT31_08295"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPW95167.1"
                     /translation="RQVSAVDIQPEIISGLKKILADKNITNVHPIEADMTKIPLPDSS
                     ADLVLMSQVLHHASSPWEALKEGVRLLKPSGTLALLDLAQHNEEELRETHGHIWLGFD
                     QKRIEFLLKDLPCKILATEVITSEECEHKKLPAICFIVKKNP"
BASE COUNT         1009 a          578 c          707 g          932 t
ORIGIN      
        1 tgcttcggct aaaataattt gttcgcggta agggaaccca gctattaagg tgtcaattgc
       61 tttggcatca tctaagactg tacttgaacc atagaggaat gcttctccca tcgctgtagg
      121 gttaggcata taagtagtgc cttggcgcgt atcaaaaatc acggccccaa tattcatcaa
      181 atctaagaat ggttttggag cttgtgggtc agaaatccca atcatatagt tcgcgttatt
      241 ttgaccacct ctaaaagcac ggtagctcgc gagttcgtta tcatggaacc cgtcggcgtt
      301 tctcatttta taaatcggga acgcgtttcc accaagggct tctgacctag aaagagaaag
      361 cacgcgagcg gggtggcttg ggttttcact attttgtttt atggcatgaa ttacaggatt
      421 attaggttgg atatatttat tgtattctgt gttttgcaca aatacgccat tgatggtata
      481 gagttctatg gttgctaaaa tggctagcag aagcactttt ttataagatt caaaacgttg
      541 gcttaagata aaccaagctg cgaccatagt gaatacaacg gtgataaatc ctggaattac
      601 ttttgcactg gtgaagttca ttaaattggt atttaaaggc ttaaaataag gcgctgtgac
      661 aggatccatc aataggtttt gacccgaaag cataaatatg ccgagtagta agaatggaac
      721 tagtattaag cccagttcta tcaaagaact tccattccat ttgttcttaa tcgcagaaat
      781 aaaattttga aaggaaagag tttctttgcg atcggtgata ttgatgacgg tgataaagat
      841 aaaaccataa agaatagaaa ctacaagacc cacagggcca aggaataatt cccactggta
      901 tctcgcgatg agtgtgatga tggtgaggag agcaaaaagg aatagcccat ttaagaatgg
      961 cttttttgac tctgggtctt tgaacgcctt gagtacaggc gctgccatca tgaccattac
     1021 gagcgggaac cagaataacg ccatgcttgg ggctctaaag ttttttacac caggtaaaat
     1081 ttcaaaccat aatttaaaga gaggggagtg agcgcccatg gcataagaaa gagctaaggt
     1141 ggcacctaag aaccagaaaa tggcaaatcg ttttttgttt ggaatgaaaa agcaaaggaa
     1201 cgccaagaat gtgaggagcg ctccagaact atcatggttt aacttaaagt gattatgccc
     1261 ccagtagaaa ggtgagcctt gaatccccca attttgatag tctttcatgg agatattcac
     1321 atgagaagag cctttgagat cacctgtttt ttgatcttgc tcgtatacat cgatgccaat
     1381 aaatccaggg agtaaaatgg aggcgagttc ttcttgatgt aaagaccagc tgacggcatg
     1441 accatagttt gttttttcat cggtaccgcg cacagattgt tttgtggtgt ataaataagg
     1501 aggaataatc tggaacgaag agatgagaag cgcaatcgca agaccaaagg ctgccatgcc
     1561 tatacgtttg actttaatag agcgtgctgc gtctaagaag aacatttcgt agagagcgta
     1621 aaggcctgct ccccaaagga acaagtaagt gagctgaaga tgagagccta agatcatcca
     1681 aaccacagaa agaacaaagg cgataaaata ttttaatctt gcttctcgta tgatttttcg
     1741 aatggataat atcgcgagtg gggcaatggc aaacaccatc atcttcccat catggccgcc
     1801 ataaacatac gtaaagtatt ggggggagaa ggcgtataga aaccccagta atccacctag
     1861 ccaccagctt tgtgtgagtg ctcgccctaa gagcatcgct gaaccaaagg ctatccagat
     1921 ggttaaaata aacttaaatc caacggctct tgctgggtct gttaaaaact ggatccaaac
     1981 gagagggtga tacacgtcgg caaataaagc atcaatcgtg gggactccac caaggcgaga
     2041 atcatcccat tctgtgacaa tcgcgttttc tgcacgaagc acgcggcttc caataccatt
     2101 taattggtcg ctattgacca ttaattttga ggaatcaaag gcaaagtccc taaagactac
     2161 caaaagaata attaaaaaga ggaaagcgag gccagctgga atgccataag attttaaaaa
     2221 agtttctttt ttcatagtcc cttgaatgga taccttatta atggatatag tgcagtaaat
     2281 ttaataagaa ccagacagat gcaggaacga gggcacttga tttgtgtgtt gtttatgaga
     2341 aggtgcgggc ggatacgtca attcggagat gttttattat cgcaaaaaga tgagccttat
     2401 tcaacgatga ggagttcttt aaacagaaaa agcggcttct aataaagaag tgccgctatg
     2461 ctttgagtat tgtaaaggct tagtctacag tattttcgtc aattttattg ctttgtatta
     2521 aatggaaaat ggagatggct gaaactacaa atacgattgc aataacagca tacttaatta
     2581 atttcttcat gtattcactc cttcagacaa ttccttttta atttacttga tttttttgta
     2641 aaaaacaagg gaaaataata atgggcttat taaataattt atttcttaag gattttctaa
     2701 aataaaacat ttttagccaa aacataaggt ttttgggcca ttcttttaaa agtttttcag
     2761 aaaggttcat ttatcaaaat taaaagaacg tcatggattt tttttcacta taaagcatat
     2821 agcagggagt tttttatgct cacattcttc gctagtgatg acttctgtag caagaatttt
     2881 acacggcaag tcttttaata aaaattcaat tcgtttttgg tcaaaaccga gccaaatatg
     2941 accatgggtt tctctaagtt cttcttcgtt atgttgagct aaatctaaaa gagcgagtgt
     3001 tccgcttggt tttaaaagac gaacgccttc ttttaaggcc tcccaagggg aagatgcgtg
     3061 gtgcaggact tgactcatta agactaaatc ggcagacgaa tctggtagtg gaattttcgt
     3121 catgtctgct tctatcgggt gtacgttggt gatgttttta tcagcgagga tcttttttag
     3181 accactaata atttctggct gaatatctac cgctgaaact tgacgg
//
LOCUS       DAOOBB010000206         3221 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_33231_length_3221_cov_5.589703, whole genome shotgun sequence.
ACCESSION   DAOOBB010000206 DAOOBB010000000
VERSION     DAOOBB010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33231_length_3221_cov_5.589703"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="PLT31_08300"
     CDS             <1..567
                     /locus_tag="PLT31_08300"
                     /inference="COORDINATES: protein motif:HMM:NF017007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HPW95168.1"
                     /translation="LIILLRNIFIFKNGNYTSLTLSALILVVITGWIYSPSIVSSSST
                     AALLFFTAPAIFLMAETFFCLVFLLFESPTFLHPTSVKPIQKNSIALIAFFLGGIGIH
                     DFMLGNKKRGIGKIILFLSSSLIITLLFLWIWIVRDLFQIANGTYPVEKGVLIGSNQT
                     ALNLAKIYLVLGIIYLLSAFASFIFAFA"
     gene            complement(583..>3221)
                     /locus_tag="PLT31_08305"
     CDS             complement(583..>3221)
                     /locus_tag="PLT31_08305"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HPW95169.1"
                     /translation="INTEQFNQAKTEILQLLKKYPKSSLLHSALSENYRRENNKALAD
                     DYMEKAYRLDSSNADAWNSRFSQIVGSQDIQVADTFYNNCPSQIYKTPEILLTRAMID
                     AQLGRQMEFFSVMDNLANNHYNNTLVVETLMNVFEQISSPSKTDLLLKKVSREYSNHS
                     SFISFLFQWHKMRGRNDEAVQLLQSFLKKYPKHTQFYNLLYSHFTGLQEYEKAAQAMQ
                     GLLKVNPFISETYIKLGEAQLALGDTAAAIQSYKKALEADYAEFAASNRLQEIQKRTK
                     WSDLATSFSIDSLRVLSKKFSKGKNKNSLFLLSQRSVIIHPTGAYETIDHSVIEVLDQ
                     KGIDEWKEYTIPLYSNDEIATVMHAHIVKSDGKTINAERNKRQLVFINLQAGDLIDIK
                     ISRKYNMTGQLAKQYESFFYMGGSLPRALSYFEIIAPDTLKYNIKQEKQNIKPVIKKL
                     MDGYSKKIFRNSQVPGLTSEEKMGPADINLPWIQISSFSNWADISHWFNTLSRGKSIV
                     TKEIEHVADSLFAKAKTPAEKIKKVHLYITEKIRYSYMPFRQSGFVPQEAATTLATQV
                     GDCKDMAVLAQTMLRRGGISSKLVLVQTRDDGGALLLPSIHFNHVILKVDSLFIDFTT
                     PDNHVNTLPWIDQNTRALVVDSTENTSLFLIPLSSPALDFVHTYTEDTLLSDGTLLRK
                     IETKKGGNFTLSDRKQFKYESTEELNKSFLTMVRNRYPQATISELKYSKGQNADSLIT
                     YSYKVKATNAAYTGNKSIAFVLPWNYKIEGSLIPGETTRQYIFQTFRSWSLWGEKKYT
                     MRLQLPTGYSILDMPQAVSMSNEFGSYQTSYTIQGQTLTATRFLSLYSQDVIPEKYAA
                     YRQFLEQIAESDKGLLILKKNE"
BASE COUNT          959 a          608 c          504 g         1150 t
ORIGIN      
        1 ttgataattc ttctacgtaa catctttatc tttaaaaatg ggaattacac ctcactcaca
       61 ttatcagcac ttattcttgt tgttataact ggatggattt actccccctc aatagtcagc
      121 tcatcttcga ccgctgcact tctttttttt acagctccag ctattttttt gatggcagaa
      181 acattctttt gtttagtttt tctgcttttt gaaagcccca cttttctaca cccaacttca
      241 gtaaagccta ttcaaaaaaa ttctatcgca ttaatcgcat tctttcttgg cggcataggc
      301 attcatgatt ttatgttagg caataaaaaa cgcggtatag gtaaaatcat tttattcttg
      361 tctagtagtc ttatcattac attgcttttt ctctggattt ggattgttcg tgatcttttc
      421 caaatcgcta atggaaccta tcctgtcgaa aaaggtgttc ttataggaag caaccaaaca
      481 gccctaaacc tagctaagat ttacctagtt ttagggatta tttatttact gtctgctttt
      541 gcttctttta tttttgcttt tgcttaatga aaagagaatg atctattcat tcttttttaa
      601 aatcaataaa cccttatcac tttcagctat ttgttctaaa aactgacgat aagcagcgta
      661 cttttctggt attacatcct gactatacaa acttaaaaag cgcgttgcag ttaatgtttg
      721 gccttgaatc gtataactag tttgatacga accaaattca ttactcatac ttaccgcttg
      781 aggcatatct agaatagagt atccagtggg taactgtaaa cgcatagtat atttcttttc
      841 accccagaga ctccaacttc taaaggtttg gaaaatatat tgacgagtgg tttctcctgg
      901 aataagactt ccttctattt tataattcca tggtaaaaca aaagcaatcg atttatttcc
      961 cgtatatgct gcgtttgtcg ctttcacttt ataagaataa gtaattagac tatccgcatt
     1021 ttggcccttg ctgtacttaa gttcacttat tgttgcttgt ggataacgat tacgtaccat
     1081 tgttaaaaat gatttattga gttcttcagt gctttcatac ttaaactgtt ttcgatccga
     1141 caaggtaaag ttgcctcctt ttttcgtttc tattttacgc agaagagttc catctgataa
     1201 caacgtatct tcagtatacg tgtgaacaaa atctaacgct ggagaagaaa gagggattag
     1261 aaacaatgat gtattttcag tcgaatctac cactaacgct cttgtatttt gatctatcca
     1321 aggaagagta tttacatgat tatcaggagt tgtaaaatca ataaataaag aatcgacttt
     1381 tagaatcaca tgattaaaat gaatggaagg taaaagtaat gcacctccat catctcttgt
     1441 ttgcacaaga actagcttac tcgaaatacc gccacggcgc aacatcgttt gcgcaaggac
     1501 tgccatatct ttgcagtccc ccacttgcgt tgctagtgta gtggcggctt cttgtggaac
     1561 aaatccagat tgtctaaaag gcatataact ataacgtatt ttttctgtta tatacaaatg
     1621 aactttttta atcttttctg ctggtgtttt ggctttagca aataaactat ctgccacatg
     1681 ttctatctct ttagtgacaa tacttttgcc tctacttaga gtattaaacc aatgactaat
     1741 atctgcccaa ttagagaagc tactaatttg aatccaagga agattaatat ctgcaggccc
     1801 cattttttct tcagaagtca acccaggcac ttgactatta cggaatattt ttttggagta
     1861 cccatccatt aatttcttta taacaggttt aatattttgt ttttcttgct ttatattata
     1921 cttcagtgta tcaggagcaa tgatttcaaa ataagagagc gcacgaggta atgaacctcc
     1981 catataaaag aaagactcat attgttttgc caattgccca gtcatgttat attttcgact
     2041 gattttaatg tcaattaaat cacccgcttg aagattaata aaaactagct gtcttttgtt
     2101 gcgctccgca ttaatagttt ttccatctga ttttacaata tgagcatgca tcactgtagc
     2161 tatttcgtca ttactataaa gaggaatagt atactctttc cactcatcaa ttcccttttg
     2221 atctaagacc tctatcacag aatgatcaat agtttcatac gctccagtcg gatgaataat
     2281 tactgatctt tgtgaaagta agaacaaaga atttttattc tttcccttgc taaacttttt
     2341 gcttaacact cgcaaagaat caatggaaaa tgaagtagct aaatcactcc actttgttct
     2401 tttttgtatt tcttgcaaac gatttgaagc cgcaaattcg gcataatctg cctctagtgc
     2461 ttttttataa gactggatag cagccgcggt atcacctaaa gcaagttgag cttctccaag
     2521 tttaatatag gtttcactaa taaaaggatt tacttttaag agaccttgca ttgcttgagc
     2581 tgctttttca tactcttgta atcccgtaaa atgggaatat aaaagattat aaaactgagt
     2641 atgttttgga tacttcttta aaaacgactg taataactga acggcttcat cattacgacc
     2701 acgcatttta tgccattgaa ataaaaagct gataaaactt gagtgatttg aatactcacg
     2761 gctcactttt ttcaataaca aatccgtttt cgaaggactt gatatctgtt caaaaacgtt
     2821 catcaatgtt tctaccacaa gagtattgtt ataatgatta tttgcaagat tatccattac
     2881 agaaaagaat tccatttgtc gacccaattg agcatcaatc atggctcgag tcagaaggat
     2941 ctcaggcgtt ttataaattt gtgatggaca attattataa aaagtatctg ctacttgtat
     3001 gtcttgagag cctacgattt gactaaatcg agaattccaa gcgtctgcat tcgatgaatc
     3061 caatcgataa gccttttcca tataatcatc tgctagtgct ttattgtttt ctcgtctata
     3121 attttcagat aaagccgaat gcaacaaact actttttggg tattttttca ataactgcaa
     3181 tatctctgtt tttgcttgat taaattgttc tgtattaatc c
//
LOCUS       DAOOBB010000207         3150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_34142_length_3150_cov_4.465267, whole genome shotgun sequence.
ACCESSION   DAOOBB010000207 DAOOBB010000000
VERSION     DAOOBB010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34142_length_3150_cov_4.465267"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="PLT31_08310"
     CDS             complement(<1..467)
                     /locus_tag="PLT31_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95170.1"
                     /translation="MEEELISNDPGVSGLIKEKTGKILFDLVTTIPESLMCPTEAPEK
                     KIQKLIQKSSVKTAILSATLAIPAGSVGILTIIPDLTAIWRIQAQLVADIAATYGQIA
                     VLSREAMVWCLFRHTASQLVRDIAVRTGSRLIMQKVSVSALRALLQKIGIQASS"
     gene            complement(536..862)
                     /locus_tag="PLT31_08315"
     CDS             complement(536..862)
                     /locus_tag="PLT31_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95171.1"
                     /translation="MGFRDEALRIALKQNKTLQKLGTIHSLNINTDTGKIELILGLKG
                     EPSPIQFEAYYEIIVEDNQTFAQISKITSEKEWISQILNLWLENNGPIRQELKGFTGG
                     LAKIFF"
     gene            complement(865..1626)
                     /gene="map"
                     /locus_tag="PLT31_08320"
     CDS             complement(865..1626)
                     /gene="map"
                     /locus_tag="PLT31_08320"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPW95172.1"
                     /translation="MAKIKIKSPKEIELIRDAGALAAETLIRAGELCKPGVSTLEIDN
                     FVGEYTKKHGGISACLGYHGYPKNCCISLNEVVCHGIPSAEIILKDGDIVNIDITTIL
                     SGYHGDTSAMFLVGQVSYEAKELVSVAKECMDAGIQAAGEPLAKYLDIGNAIQGIAEE
                     YGYSVVEDYCGHGIGRGFHEEPTILHFRNNELKAMIEPGHVFTIEPMINMGRYATKTL
                     KDGWTAVTKDGSLSAQFEHTVARTKNGIEILTLAR"
     gene            complement(1613..2998)
                     /locus_tag="PLT31_08325"
     CDS             complement(1613..2998)
                     /locus_tag="PLT31_08325"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPW95173.1"
                     /translation="MSENTQEKINPFLTPIQIIEPKNKLPDYTFDQLPSLQKEVLTAH
                     GWTDLMPVQRKAIPYMLSARDMLIQSKTGSGKTGAFVLPLLQVIEPTHLFPQALILVP
                     TRELALQVQSEIELLAKGSGIRSTPIFGGVGYEPQLRALREGVHIIVATPGRLLDHAQ
                     RGHIDFLSVRDLIMDEADEMLSMGFYPDMQRIRKYLPKDIACTMFSATIPQTVKSLAR
                     EFQRPNADFLSLSYDQVIANNLEHRYYMCDVMEKDTLTIKVLEWENPESCMIFCNMKK
                     DVAYLEQVLTNYGFVVGALSGDVPQKQRESILNNFRTKKLPILICTDVAARGIDVSHV
                     THVIVFDHPDDHEVYVHRSGRTARAGRSGVAISLITPVEEIELQKTAADFGIQFSKKP
                     PITEEQIAKKIRERTRIYLERHKRTLGQRSQERMRRYLPLVEELSQIEEDKELLAFLL
                     DRYYWNQYGKN"
BASE COUNT          885 a          609 c          647 g         1009 t
ORIGIN      
        1 gatgacgctt gaatccctat tttttggaga agcgcacgta atgccgatac agagaccttt
       61 tgcatgatta gtcgacttcc tgttcgtaca gcaatgtctc gaacgagttg agaggctgtg
      121 tggcgaaata agcaccatac cattgcttct cggctgagaa cggctatttg cccgtatgtg
      181 gcagcaatat cagcaactaa ttgagcctga attcgccaaa tggctgttaa atcagggata
      241 atggtgagta tgcctacaga ccctgctgga attgctagag tggcactgag tatggctgtt
      301 ttgactgaag atttttgaat taatttttga attttctttt caggtgcttc tgttgggcac
      361 atcaaagatt ctggaatagt tgtcactagg tcgaaaagta ttttacctgt tttctctttg
      421 ataaggccag aaactccagg gtcatttgaa attaattctt cttccataat atttccttgt
      481 ctttcttata atataacact cttttagtca taaaaattca aaagactggg gctcctcaga
      541 aaaatatttt cgcaagtcct ccagtgaatc cttttagttc ttggcgaata gggccattat
      601 tttcaagcca gagattcagg atttgagaaa tccattcttt ttcagaggtg atttttgaaa
      661 tttgtgcaaa tgtttgatta tcctcgacaa tgatttcata gtaggcttca aattgtatag
      721 gagaaggttc tccttttaaa cccagaatca attcgatttt tcctgtatcg gtattgatgt
      781 ttaaagaatg tatagtgcct aacttttgaa gcgttttgtt ttgttttaaa gcgattctta
      841 aggcctcgtc acgaaatccc atgattacct tgcaagcgtt agaatttcaa ttccattttt
      901 agtgcgagca acggtatgtt caaattgagc tgagagtgat ccatctttag tcactgccgt
      961 ccacccatct tttaaggttt ttgtggcata gcgccccata ttgatcatgg gctcaatggt
     1021 aaaaacgtgt ccaggttcta tcatggcttt taattcattg tttcgaaaat gtaaaatggt
     1081 cggttcttcg tggaaacctc ggccaatccc gtgaccacaa tagtcttcta ctacgctata
     1141 gccgtattct tcggcgatgc cttgaattgc gtttcctatg tctagatact tggcaagagg
     1201 ctctccagcg gcttgaatgc ctgcatccat acattcttta gcaacagaca ccaattcttt
     1261 ggcttcgtac gaaacttggc ctacaagaaa catagcgcta gtgtctccat gataaccaga
     1321 aagaatagtc gtgatatcaa tgtttacaat atcaccatct tttagaataa tctctgcact
     1381 agggatcccg tggcaaacga cttcgtttaa gctgatacaa caatttttgg ggtaaccgtg
     1441 gtagccaaga caagcagaaa taccaccatg tttttttgtg tattctccaa cgaaattatc
     1501 tatttcaagc gtggaaacgc ctggcttaca gagctctcca gcacgtatta atgtttctgc
     1561 agcaagagca ccagcatctc gaattaattc aatttcttta ggtgatttaa tcttaatttt
     1621 tgccatattg attccaataa taacggtcta ataagaaagc taaaagttct ttgtcttctt
     1681 cgatttgaga aagttcttcc actaaaggca aatagcggcg catgcgttct tgactacgtt
     1741 gccctagtgt tcttttatgg cgttcgaggt agatacgagt acgttctcta atttttttgg
     1801 caatttgttc ttctgtaatt ggaggctttt tggaaaactg aattccaaaa tcggcagccg
     1861 ttttttgaag ctcaatttct tccactggag tgattaaaga aatagcgacg ccgcttctgc
     1921 ctgcacgggc agttcgaccg cttcgatgaa catagacttc atgatcgtct ggatgatcaa
     1981 agacgatgac atgagtgacg tgagaaacgt cgatacctct tgctgccaca tcggtgcaaa
     2041 taaggatagg aagttttttc gttctaaaat tatttagaat cgattctctt tgtttctgag
     2101 ggacatctcc agaaagagct cctacgacaa aaccgtaatt agtgagaact tgttctaaat
     2161 aagccacatc ttttttcatg ttgcaaaaaa tcatgcaact ttctggattt tcccactcta
     2221 aaactttaat tgttaaagtg tctttttcca tcacatcgca catgtagtag cggtgttcta
     2281 ggttatttgc aatgacttgg tcataactca aggataaaaa gtcagcatta ggacgttgga
     2341 attcacgagc aaggcttttt actgtttgag gaatagtagc actaaacatg gtgcaagcga
     2401 tatcttttgg aaggtatttg cgaatgcgct gcatgtctgg gtaaaaaccc atagaaagca
     2461 tttcatcggc ttcatccata attaagtctc ttacagaaag aaagtcgatg tgccctcttt
     2521 gagcgtgatc taataagcga ccaggtgtgg cgacaatgat atgaacgcct tcgcgtaaag
     2581 cacgaagttg aggctcgtac cctacaccgc caaaaatagg ggtagaacgg atgccagaac
     2641 ctttcgcgag aagttcaatt tcactttgta cttgtaaggc gagttctcta gtgggcacta
     2701 aaataagggc ttgtgggaac aaatgtgtcg gttcaatgac ttgtaaaagg gggagaacaa
     2761 aggctcccgt tttacctgaa cctgttttag attggattaa catatcccta gcacttagca
     2821 tataagggat agcttttctt tgaacgggca ttaaatcggt ccagccatga gctgtaagaa
     2881 cttctttttg aagagaaggt aattgatcaa atgtgtaatc ggggagttta ttttttggct
     2941 cgataatttg aatcggagtt aaaaatggat ttattttttc ttgtgtattt tcactcatat
     3001 tgacaaaaag tagaaaaaag aaatagaaac tactttattt tatgctccaa tagtattcta
     3061 ttcctatact tgcaaagcaa tcaaccgtag atccacctat aatagtgggc tctgaagcgg
     3121 cttctgaaga aggatcactg tctaacatac
//
LOCUS       DAOOBB010000208         3137 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_34335_length_3137_cov_4.039260, whole genome shotgun sequence.
ACCESSION   DAOOBB010000208 DAOOBB010000000
VERSION     DAOOBB010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34335_length_3137_cov_4.039260"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="PLT31_08330"
     CDS             <1..1083
                     /locus_tag="PLT31_08330"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HPW95174.1"
                     /translation="SNIMITQDNRVYIMDFGISYIQEMEKERLTMTGMTMGTPEYMSP
                     EQCHGDEITIQSDIYSLGVILFEMTCGRLPFTGNKPIEIAIKHVQEKPPVPDTLRQDV
                     PQELSFLILKCLKKNMNERYRDMQELLDALDLIFPKEGTSSQNVIRPKKRITLAHSNK
                     TFFSSASKISSEIKRKKLMILGILMFPLLIMLLIMLMLTHKPASVLQQITDVNLRASF
                     EEKNLEGDSPSGYPPENLIDQNIKSAWLFAPPVNIKNPILVIEFPEPTIITHFGMAIG
                     YQKSIDDHFGDRFRVFNKPKSLILKTKEGIKQKIVFDNIKGMQYPTINAIETSELQIF
                     LEDSYELESSKDFAISEIRLLGLKLK"
     gene            1080..1484
                     /locus_tag="PLT31_08335"
     CDS             1080..1484
                     /locus_tag="PLT31_08335"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HPW95175.1"
                     /translation="MKDHSNNTKKGRIAETYAAAYLRRQNYTLLSRNYSFAGGELDII
                     AETPEGETVFTEVKSVWNTKKGSASYRVSRRKQIKIWKTACHFLHFHGGENKRCRFDV
                     IALDLNNINFKLTHFKNAFDGPQTFHECQYAF"
     gene            1474..2061
                     /locus_tag="PLT31_08340"
     CDS             1474..2061
                     /locus_tag="PLT31_08340"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPW95176.1"
                     /translation="MRFEVHPINPQARTVKLAASLLEDDGLLLYPTESGYAIGCNAES
                     SKAIQKLYALKKPLKKFLMALILPDIRSATDYAHVSNFAFNIMKPRIPGPYTFILPAA
                     PHIARKLDVKRTEIGVRFPTHPFFKELFQHYNKPILSTAAKISEEELTDPDALWDLFQ
                     HSVDMMIDCGEIEICPTNVISLVGNDIEIIRGELL"
     gene            complement(2116..2562)
                     /locus_tag="PLT31_08345"
     CDS             complement(2116..2562)
                     /locus_tag="PLT31_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95177.1"
                     /translation="MTPIMNSLACLFALLPSYFLWSSSRSIARQGVLLALVLCSFMIF
                     TFLVGDMESPLMEFYPLKMWAFCLCVSTLTLTKKQVFFMSLAQGFWLWILFFGSLSLA
                     YRGETVSYLSMILLLAGILFVQFFKNKPKEISLGILIYWVGIWMLF"
     gene            complement(2559..>3137)
                     /locus_tag="PLT31_08350"
     CDS             complement(2559..>3137)
                     /locus_tag="PLT31_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HPW95178.1"
                     /translation="GMIFKIIVPAIIITILFWFLNYRFDSTVSVINWVPALFPVLSLI
                     VGLSFFVIELFSIVSKPRFYLVSGACLLVLGFLMGAFYAGGDTNLGRENKIISVEQTS
                     EKERSTFDLWKSRCASCHGLDGEGNRKFIYEFYPEIQDLTLKRLNLQGEDSLTQVVLK
                     GRAFMNPFKDRLSESEARALIRFMYKLAEEKK"
BASE COUNT         1116 a          577 c          522 g          922 t
ORIGIN      
        1 tctaatatta tgattacaca agataatcgt gtgtatatca tggattttgg catctcttac
       61 attcaagaaa tggaaaaaga gcgtttaacc atgacgggca tgacgatggg aactcctgaa
      121 tacatgtctc ccgagcaatg tcatggcgat gaaattacga ttcaatccga tatttatagt
      181 cttggagtga ttcttttcga aatgacttgt ggaaggcttc cgtttacagg caataaacct
      241 atagaaattg caatcaaaca tgttcaagaa aagcctcctg taccagatac cctcaggcaa
      301 gatgttcctc aagaactctc ttttttgatt ttaaaatgcc ttaagaagaa tatgaacgag
      361 cgctatcgcg atatgcaaga actcttagat gcgcttgact tgatttttcc aaaagaaggt
      421 acatctagtc agaatgtgat tcgtccaaaa aaaagaatca ctttagctca ctcgaataaa
      481 acatttttct cttctgcatc caaaatttct agtgaaatca aacgtaaaaa gctaatgatt
      541 cttggcattt taatgtttcc attattaatc atgctattaa tcatgttgat gcttactcat
      601 aaacctgcaa gtgttcttca gcaaattact gatgtaaact tacgcgcttc ttttgaagag
      661 aaaaatctag aaggagattc tccttctggg taccctcccg aaaatttaat cgaccaaaat
      721 atcaaatcag cttggttatt tgcccctcct gtgaatatta aaaatccgat tttagtgatt
      781 gaatttcctg aaccaactat aatcactcat tttggaatgg ctattggata tcaaaaatcc
      841 attgatgatc attttggaga tcgtttccga gtttttaata agccaaaatc tctcatatta
      901 aaaacgaaag aaggcattaa gcaaaaaatt gtttttgata acattaaggg aatgcaatac
      961 ccaacgatta atgcgattga aacaagcgag ctccaaatat ttcttgaaga tagctacgaa
     1021 ctagaatctt ctaaagattt tgctatatct gaaatacgtt tgcttggact aaaattaaaa
     1081 tgaaagacca ttccaataat accaaaaaag gccgtattgc agaaacttat gctgccgcct
     1141 atttaagaag acaaaattat actttacttt caagaaatta ttcttttgct ggtggagaac
     1201 ttgatatcat tgcagagaca cctgaaggcg aaacggtttt tacagaagta aaatcggtat
     1261 ggaacaccaa aaaaggaagc gcctcttatc gagtctcacg tagaaaacaa atcaaaatct
     1321 ggaaaactgc ttgtcatttt cttcattttc atggaggaga gaacaagcgt tgccgttttg
     1381 atgtgattgc tttagattta aataatatta attttaaatt aactcatttc aaaaatgcct
     1441 ttgatgggcc acaaactttt cacgagtgtc aatatgcgtt ttgaagtaca tcctattaat
     1501 cctcaagcga gaacggttaa actcgctgca tctcttttag aagatgatgg tcttctcctt
     1561 tatcctacag aatctggcta tgccattggc tgtaatgcag aatcctcaaa agcgattcaa
     1621 aaactctatg cattaaaaaa accattaaaa aaattcttaa tggcattaat tctccctgat
     1681 attcgatcag ccacagatta tgcacacgtt agtaattttg cttttaatat catgaagcca
     1741 aggattccag ggccatatac ctttatttta cctgcagctc cccatattgc tagaaagcta
     1801 gatgtgaaaa gaacagaaat tggtgttcgt tttcctacgc atccattttt caaagaatta
     1861 tttcaacatt ataataaacc catactaagt acagcagcta aaatttcaga agaagaatta
     1921 acagatccag acgctctatg ggatttattt caacatagcg tagatatgat gattgattgc
     1981 ggcgaaatag aaatttgccc cacaaacgtg ataagtctag ttggtaacga catagaaatc
     2041 attcgtggtg aactattgta aaacagtttt tattgctttt gacaatctat tctcgctaaa
     2101 aaatgagaat gttttttaaa aaagcatcca aataccaacc caatagataa gaatacctaa
     2161 tgaaatttct ttaggcttat tcttaaaaaa ttgaacgaaa aggatgccag caagtagaag
     2221 aatcattgat aaataactca cggtttctcc acgataggct agagaaagtg agccaaagaa
     2281 aagaatccag agccaaaagc cttgagctaa agacataaag aaaacttgtt tcttagttaa
     2341 cgttaatgtt gaaacacata agcaaaaagc ccacattttt aagggataaa actccatcaa
     2401 agggctttcc atatctccta ctaagaacgt aaagatcata aaactacaca aaactaaggc
     2461 gagtaaaaca ccctgacggg caatggaacg agaagacgac cacaaaaaat aagagggtaa
     2521 taaagcaaaa agacaagcta aggaattcat gattggagtc attttttttc ctccgctagt
     2581 ttgtacatga agcgaattaa tgctctcgct tccgattctg agaggcgatc cttaaatgga
     2641 ttcataaatg ctcggccttt taaaacgacc tgagttaagg aatcttcccc ttgtaaattc
     2701 aatcttttta acgtgagatc ttgaatttct ggatagaatt cgtagatgaa ttttctgttt
     2761 ccttctccat caagcccatg gcaagaagca caacgacttt tccacaaatc aaaagtagag
     2821 cgttcttttt ctgatgtttg ttctacagaa attattttgt tttctcttcc taagttagta
     2881 tccccacccg cataaaaagc ccccattaga aatcccaaaa ctaatagaca agcccctgaa
     2941 acaagataaa atctgggttt tgagacaata gaaaaaagct ctataacaaa aaaagataat
     3001 cctacgatta atgataaaac tggaaataaa gcaggaaccc aattaattac agaaacagta
     3061 gagtcaaaac ggtaatttaa gaaccaaaaa aggatagtga taataatagc aggcactatt
     3121 attttgaaaa tcatccc
//
LOCUS       DAOOBB010000209         3109 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_34767_length_3109_cov_5.751801, whole genome shotgun sequence.
ACCESSION   DAOOBB010000209 DAOOBB010000000
VERSION     DAOOBB010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34767_length_3109_cov_5.751801"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1745)
                     /locus_tag="PLT31_08355"
     CDS             complement(<1..1745)
                     /locus_tag="PLT31_08355"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95179.1"
                     /translation="MKKLLLFSFLCLLGSCASKGSVNSDVTKALKQIQVPFEVVNLSD
                     SVQIRVLEKPNLASLDLAAYYQNPIKKQEDLEAPVNYKGLLASTPVKDYPFASLDSIS
                     SEAPEFLNFFMPVAFEPFVKTLASESRDSLSGLLRKIEGEKWNEPEVFKAFQRVSKLW
                     GISRNGNGEWWIEIEPASWTNRPVFWGRLKTRASSIDLESLTQYQNKELSPEEVMHWA
                     RTLTSYWYPTLNTDLELHTPGSPWNQYSPYFIIRGNPMGKVIQMAFDIKSLRMTPEAL
                     LQQEKVESTSAASDRQIDTSSLLREQTLNMLEACPLSDSVKTFQNALKNVLEKLPQEQ
                     NAFESGGMLWFRRNASYVLADEITSQDSLHNPLPRLLELKAHLDSLGIQLLVVPVPVK
                     EEIYPERLIEGTSANLCVHPNGRSFTKKMLESGLDVIDLYPILKAAKAGDQPPSFLFQ
                     RYDTHWATTGLLASLEAIAARITQYAWYEKAGPTPGFLEMRDTVVTREGDLVAHVPEN
                     QKSLFPADTFAVYKVYKNNEPYSAKKGAPILLMGDSFTGVFESVDQRSGGPGSLIAFA
                     TGLDVQVLTSWGGGPG"
     gene            1836..2702
                     /locus_tag="PLT31_08360"
     CDS             1836..2702
                     /locus_tag="PLT31_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HPW95180.1"
                     /translation="MKSKNIIMIQGQWTGDFESNNAWYKAEALKNKGQDLDLLILPEL
                     FHTPYFPFEENADFFHWAIEKDHPLVLEWREIAKELHCVIVFPFFEKRAKGIYHNSAY
                     VFERDGSIAGLYRKSHIPDDPAFYEKYYFIPGDTGFEPIQTSAGNLGILICWDQWFPE
                     AARIMSLKGADILIYPTAIGWAKNEPESIYERQKDSWMTVMRGHAIANRLFVIAVNRI
                     GTEGDLTFWGHSFVAAPDGFLLKDLPSNLLGSEKIQISFDEIEENRRWWPHFRDRRID
                     LYSDILKIWDTP"
     gene            2699..>3109
                     /locus_tag="PLT31_08365"
     CDS             2699..>3109
                     /locus_tag="PLT31_08365"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HPW95181.1"
                     /translation="MKSSSLKKIVRSPAEWEEQAATWLSFPRHPKNWAGEKGVQIRAF
                     YFNLIYNIAKFQPVKMLVPKDWELPKEEEKAFLKCRFAPQAYKIATNDIWIRDYGPFF
                     VKKGDKKEIVQTNFNAWGAKFPPFLLDNKVPSAIA"
BASE COUNT          993 a          650 c          581 g          885 t
ORIGIN      
        1 ccagggccgc ctccccagct tgtcaagact tgtacatcta accctgtcgc aaaggcaatc
       61 aaagatccag ggccacctga acgttgatcc accgattcaa acacacccgt aaaagaatca
      121 cccatcagta atattggagc tccttttttc gcagaataag gttcattatt cttataaact
      181 ttgtaaacgg caaaagtatc ggcagggaaa aggctcttct ggttttctgg aacgtgagcg
      241 actaaatcac cttctctagt aacgacagta tctcgcattt ctaaaaagcc aggcgttgga
      301 ccagcctttt cataccaagc atattgggtg attcttgctg caatcgcttc taaagaagct
      361 aacaaaccag ttgtagccca atgggtatca taacgctgaa acagaaatga aggaggttga
      421 tcgcctgctt tggcggcttt caatatagga tacaaatcga ttacatctaa acctgattcg
      481 agcatctttt tagtgaagga acggccatta ggatgaacgc ataaattagc ggaagtccct
      541 tcaatcaaac gctctggata aatttcctct ttcacaggca caggcaccac aagcaattga
      601 attcctaaag aatctaaatg agcttttaat tccaataatc gtggaagagg attatgcaat
      661 gaatcttgtg aagtaatttc gtctgctaag acataactcg cgtttcttct aaaccaaagc
      721 atgccaccag attcaaacgc attctgctct tgaggtaatt tttctaaaac attttttaga
      781 gcattttgaa acgttttcac cgaatccgaa agagggcagg cttctaacat attcaatgtt
      841 tgttcgcgga gcaaagaact cgtatcgatt tggcgatctg aagccgctga cgtagattct
      901 actttttctt gttgaagtaa agcttctggt gtcatccgaa gagacttaat atcaaaagcc
      961 atttgaatca cctttcccat gggatttccg cgaattataa aataagggga atattgattc
     1021 catggcgatc caggagtatg gagttctaag tctgtattta aagtaggata ccaataagaa
     1081 gttaaagttc tagcccaatg catcacttct tcaggagaaa gttctttatt ctggtattga
     1141 gtgaggcttt ctaaatcaat gctagaagct cttgttttta ggcggcccca aaatacagga
     1201 cgattagtcc aagaggctgg ctcaatttca atccaccatt ctccatttcc attacgagaa
     1261 attccccaaa gctttgaaac tcgttgaaaa gccttaaata cttctggttc attccatttc
     1321 tcgccttcaa tttttcttaa taaaccagat aaagaatctc gactctcaga agctaacgtt
     1381 ttcacaaaag gctcaaaggc cactggcata aaaaaattta agaattcagg tgcttctgat
     1441 gaaatagaat ctaaagaagc aaaaggataa tcttttacag gagtacttgc gagtaatccc
     1501 ttgtaattaa ctggggcttc taaatcttcc tgttttttga ttggattttg atagtacgcc
     1561 gctaagtcta aagaagccaa attgggtttt tcaagaacac gaatttgaac tgaatccgac
     1621 aaattcacca cttcaaatgg aacttgtatc tgctttaaag ccttagttac gtctgaattt
     1681 acagaacctt ttgaggcaca agaacctaaa aggcacaaaa agctgaataa gagtaatttt
     1741 ttcaccgcca caaaatagaa aaatcaagcc ccataaagaa gtgattgata ttcaaaaacg
     1801 ctctagaacc ttgtaaaaat tcctataatt acaatatgaa atcaaaaaac ataatcatga
     1861 ttcaaggcca atggacaggt gattttgaat ctaataacgc ttggtacaaa gcagaggcct
     1921 taaaaaacaa aggccaagat ttagatcttt taattttacc tgaattattc catactccct
     1981 acttcccttt tgaagaaaat gcagactttt ttcactgggc tatagaaaaa gaccatccac
     2041 tagtcttaga gtggagagaa atcgctaaag agctccattg cgtaatcgtt tttccatttt
     2101 ttgaaaagcg tgcaaaaggg atttatcata atagtgccta tgtttttgag cgcgatggaa
     2161 gcattgctgg attatatcgc aaaagccata ttccagacga tcctgctttt tatgaaaagt
     2221 attatttcat cccaggcgac actggttttg aacccataca aacttctgct gggaaccttg
     2281 gcattttaat ttgctgggat caatggtttc cagaagccgc tcgtatcatg agccttaaag
     2341 gagccgatat tttaatttac cctacggcga ttggttgggc taaaaatgaa cccgaaagca
     2401 tttacgaacg tcaaaaagat agctggatga cagtcatgcg tggccatgca atcgcgaatc
     2461 gtctttttgt catcgcggtc aatagaattg gaaccgaagg cgacctcact ttctggggcc
     2521 actcttttgt agccgctcca gatggattcc tcttaaaaga cttgccatct aatttacttg
     2581 gatcagaaaa aattcaaatc tcctttgatg aaatagaaga aaatagacgc tggtggcctc
     2641 actttagaga ccgtcgcatt gacctctatt cagacatttt aaaaatttgg gatactccat
     2701 gaaatcaagc tccttaaaga aaatagtccg ctcccctgca gaatgggaag aacaagccgc
     2761 cacttggctt tcctttccac gccatcctaa aaattgggct ggtgaaaaag gagtccaaat
     2821 cagagcattt tattttaact tgatatataa catcgccaag ttccaacccg ttaaaatgct
     2881 tgttccaaaa gactgggaac ttcctaaaga agaagaaaaa gcgtttttaa aatgccgttt
     2941 tgcacctcaa gcctacaaaa tcgccaccaa tgatatttgg attagagact acggcccatt
     3001 ttttgtaaaa aaaggcgata aaaaagaaat cgtacaaacg aattttaacg cttggggagc
     3061 aaaattccct ccgttccttt tagataacaa agtcccctcc gctattgca
//
LOCUS       DAOOBB010000210         3096 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_34938_length_3096_cov_5.081223, whole genome shotgun sequence.
ACCESSION   DAOOBB010000210 DAOOBB010000000
VERSION     DAOOBB010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34938_length_3096_cov_5.081223"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            242..2401
                     /locus_tag="PLT31_08370"
     CDS             242..2401
                     /locus_tag="PLT31_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HPW95182.1"
                     /translation="MQLEGFLKKITYQNTENGFSVLRVLVDGETKPTTVTGYLPELHV
                     GETIRMEGKWGFHPKYGNQFQCEKSELVLPSGREGFIAYLSSGLFPGIGPKTAERLVQ
                     EFKDDLFQILDNTPEKLIGAIKGFTRKRLDKFLTVWNQMKDSRETLLFLYSHQITGSI
                     AKKIWLHYGASTIETITENPYLLCEEVWGIGFVKADEIAQKVGVAKDHYMRLRAGLIF
                     TLMEAAMNEGHTYLPRTTLLEKALQILRADPTDSDFCERLFFSLDELLQEKTLENHNE
                     GIWLPFLYKAEQGIASFVRERLSSKRKPPEDSFIQALKEFEREHNLCYDPIQFDGICS
                     ALNSPLCIITGGPGTGKTTMLRGIIHLASLQKEKTILAAPTGRAARRMYELCGREAQT
                     IHRLLEIDPATGKFHRNEDYPLDASFIIIDEFSMVDTLLAAALIKAVPTKARVLFIGD
                     KDQLPSVGPGNILRELLSCPDIPAIFLTHVFRQSGGSDIAEKASRINQGYMPNPIDGT
                     HFHFHLFDTPEEGKELLKKIITEDLPGKLSIDPLQNLQVLSPMRKGPLGIFELNRFLQ
                     KILNPKPTGLSILGVDWKNGDRVMQLKNNYDKNVFNGDIGFIKQVNKDSQSLSIDFEG
                     KIMRFETLELDQMNLAYACTIHKSQGSEYPAVIIILDQSHYMMLQRNLIYTAITRAKG
                     HVWILSAPGAFHTAVRNNRSVKRFTLLAESIKDGFNQ"
     gene            complement(2503..2643)
                     /locus_tag="PLT31_08375"
     CDS             complement(2503..2643)
                     /locus_tag="PLT31_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95183.1"
                     /translation="MNKKLWILTISAFFSLIALAGCSGDAAASIYSGNDSYVNTDFIE
                     GE"
     gene            complement(2661..>3096)
                     /locus_tag="PLT31_08380"
     CDS             complement(2661..>3096)
                     /locus_tag="PLT31_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95184.1"
                     /translation="IAPYINVAMKVLGNDRARVLVGSNNTLGFQMFDKIDNVTDGAVE
                     FGLFLAPNIFAEIALTKNWMFFGGASHNLNLFTYSSKSIGNTSSSVLQMLTEQTIVQI
                     GSRFQWKSIALEASLADAFLENPFGSFNGGTLAFNLGAFIYF"
BASE COUNT         1047 a          643 c          552 g          854 t
ORIGIN      
        1 cccccttttt tttctctttt tgctaccgtc tgattcactt caaccctctt taacggagat
       61 acgatatgcc ttgcggaaaa aaaagaaagc gcaggaagat cgcgacccac aaacgcaaga
      121 agcgtcaacg tcgtgaccgt cacaaaaaga aaattcgcta atttccgtta gcattcttag
      181 tgatcctgaa taaaagagaa tatttaaaaa tattctcttt tgtttttgta tacttgttct
      241 gatgcagcta gaaggttttc tcaaaaaaat cacttatcag aatacggaaa atggattctc
      301 tgttttacgt gtcttagtag atggagaaac caaaccaacc acggtcactg gttatttacc
      361 tgaactccat gtcggagaaa ctattcgtat ggaagggaaa tggggatttc atcctaaata
      421 cggcaaccaa ttccaatgtg aaaaaagcga actcgtttta ccttcgggca gagaaggctt
      481 tattgcatac ctatcaagcg gtttattccc tggaataggg ccaaaaacag ctgaacgact
      541 tgtccaagag tttaaggacg acctatttca aatcttagac aatacccctg aaaagcttat
      601 aggagcaata aaaggcttta cacgcaaacg gctagataag tttttaaccg tttggaatca
      661 aatgaaggat agtcgtgaga ccctcttatt tttgtactcg catcaaatta cagggagtat
      721 cgcaaaaaaa atttggctcc attacggggc ctcaaccata gaaaccatta cagaaaaccc
      781 ttatctttta tgtgaagaag tatggggtat aggctttgtc aaagcagatg aaatcgcaca
      841 aaaagtaggt gtcgctaaag accattatat gcgacttcgt gctggcctta ttttcacctt
      901 aatggaagcg gccatgaatg aagggcacac ttatttaccc cgaactacgc ttttagaaaa
      961 ggccctccaa attttacggg cagaccccac cgattctgat ttttgcgaaa gattattctt
     1021 ttctcttgac gaattactcc aagaaaaaac tcttgaaaac cataacgaag ggatctggct
     1081 tccattttta tataaagcag aacaaggcat cgcctcgttt gtcagagaaa gattatcttc
     1141 aaaaagaaaa cctcctgaag actcttttat tcaagcatta aaagaattcg aaagagagca
     1201 taatttatgc tatgatccta ttcaatttga cggcatttgc agtgcattaa atagtcctct
     1261 atgtatcatc acaggaggcc caggaactgg aaaaacaacc atgcttcgag gcatcatcca
     1321 tttagcctct cttcaaaaag aaaaaaccat ccttgcagcc cccacaggca gagccgcgag
     1381 aagaatgtac gagctttgcg gacgagaagc tcaaacaata catcgacttt tggaaatcga
     1441 tccagctacg ggaaaatttc accgcaacga agattatcct ttagatgcaa gtttcattat
     1501 cattgatgaa tttagcatgg tggatacttt gcttgcggcc gctttgatta aagctgttcc
     1561 taccaaagca cgcgttttat tcattggaga taaagatcag ctaccaagcg ttggccctgg
     1621 taatattctt cgcgaactac tctcttgccc agatatccct gccatattct taacacacgt
     1681 ctttaggcaa tcaggcggca gcgatattgc agaaaaagca agccgcatca atcaaggcta
     1741 tatgccaaat cctatagacg ggacccattt tcattttcac ttatttgata cacctgaaga
     1801 aggaaaagaa ctcttaaaga aaataataac tgaagacctt ccaggaaaac tcagtataga
     1861 tccgcttcaa aaccttcaag ttctttctcc catgcggaaa gggcctcttg gcatatttga
     1921 actcaatcgt ttcttacaaa aaatattaaa tccaaaacct actggacttt ctatacttgg
     1981 tgtcgattgg aaaaatggcg atcgtgtcat gcagctaaaa aacaattacg acaaaaatgt
     2041 ttttaacgga gatattggct ttataaaaca agtgaataaa gattcgcaat ctttaagcat
     2101 cgacttcgaa ggcaaaataa tgcgctttga aaccttagaa ctagaccaaa tgaatctcgc
     2161 ctatgcttgc accattcata aaagtcaagg aagcgaatac ccagcagtaa tcatcatact
     2221 agatcagtct cattatatga tgctacaaag aaacttaatc tacacagcaa tcacaagagc
     2281 aaaagggcat gtatggatac tctctgcccc aggcgccttt cacacagctg taagaaacaa
     2341 tcgttctgta aaacgtttta ctctattagc tgaatccata aaggacggct ttaaccaatg
     2401 ataaagcatt ttttacgagt atcagcacca aggttatctt tattcctaaa aacaaaaaaa
     2461 gctccagtca aaactggagc ttttttatta aaaaactaat tcttattcac cttcaataaa
     2521 gtctgtgttg acataagaat cattaccgct gtatatagat gcagcagcat caccagaaca
     2581 gccagctaaa gcgattaagc taaagaaggc agaaattgta agtatccata attttttatt
     2641 cattgttata ccctcttata ttaaaagtaa ataaatgcac caagattaaa tgctaaagta
     2701 ccaccgttaa atgagccaaa cggattctca aggaatgcgt cagctaagct tgcttcaaga
     2761 gcaatactct tccattggaa gcgagatcca atttgaacaa tggtttgttc tgtaagcatt
     2821 tgtagaactg aagaagaagt attaccgata gatttggacg aataagtaaa caagttcaag
     2881 ttatgagaag caccaccaaa gaacatccag ttttttgtta atgcaatttc tgcaaaaata
     2941 tttggagcta agaaaagacc aaactcaaca gctccatctg ttacattgtc gattttatca
     3001 aacatttgga aaccaagagt attatttgaa ccaactaata cgcgtgcacg atcgtttcct
     3061 aatactttca tagccacatt aatatacgga gctatt
//
LOCUS       DAOOBB010000211         3009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_36273_length_3009_cov_3.907244, whole genome shotgun sequence.
ACCESSION   DAOOBB010000211 DAOOBB010000000
VERSION     DAOOBB010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36273_length_3009_cov_3.907244"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="PLT31_08385"
     CDS             <1..787
                     /locus_tag="PLT31_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HPW95185.1"
                     /translation="YDTFNAFSDQHPWVEKENVQPKSIAREMFENFSTFSSYIKDYSL
                     QRSEALLLRHINGVYKVLSQTVPPAFKNEELIEMQDYLRDMIQRVDSSLLEEWERMRH
                     PEFKAEEVKELGAFGAEKALEDITYDKKRFLGMARQRIFALMTLLQKQKYAEVLDLLS
                     EDLNEGELLADASGVPWTEKRLLETMALYVAEHDRFLLDVEGRSLKHTLVEYSGDTMQ
                     IQQMLQDPNELNDWSIDFEIPLSASREAGSVLLRLCRIGEVTS"
     gene            851..1639
                     /locus_tag="PLT31_08390"
     CDS             851..1639
                     /locus_tag="PLT31_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016110865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HPW95186.1"
                     /translation="MRSDEVDYEIIGDDLQIVEIELDPTETIIAEAGSMNYMEEDISF
                     EAKMGDGSNPGAGIMDSLLNVGKRVLTGESLFITHFTNKGSQKRKVAFAAPYPGKIIP
                     IDLNQIGGRFICQKDAFLCAAYGTKLSITLNKRIGAGFFGGEGFILQKLEGDGLAFVQ
                     AGGTIIKKELRGETLRVDTGCIVGFSDGIQYDIQRSGSLKSMVFGGEGLFLATLRGVG
                     TVYLQSLPFSRLADRIIAHAPSMGGSSKGEGSVLGKLGDIFGDR"
     gene            1710..>3009
                     /locus_tag="PLT31_08395"
     CDS             1710..>3009
                     /locus_tag="PLT31_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HPW95187.1"
                     /translation="MAVLMRDMILKSGVNFGTSGARGLVSDMTDKVCYVYTKAFIQYC
                     SAHYNESKTIAVAGDLRPSTERILSALAQAAKELNTEFVYCGRIPSPAIALYGIEQKI
                     PTIMVTGSHIPDDRNGIKFNHPFGEISKKDEEGILSQSVDIDESLFDASGMLLNPPSL
                     PSIETKAIKQYTARYSAFFGPEALMGLTLGVYQHSAVGRDTIVEVLESLGAFVKPLGR
                     SEKFIPVDTEAIRPEDEALAKKWAIEGLDAIISTDGDSDRPLLADDSGEWLRGDVLGI
                     LAAKSLGINRVATPVSCNTALEKCEAFEKISRTRIGSPYVIVGMESLCEKGKTVAGYE
                     ANGGFLLATPIEKEGRILKALPTRDALLPVIAVLAEACAHRRNVSDLLRHLPKRFTSS
                     DRLKEFPTERSRQILEKIKGKNLGESLFGKFSGKLISIDET"
BASE COUNT          913 a          510 c          666 g          920 t
ORIGIN      
        1 atatgatact tttaatgcat tttcagatca acacccttgg gtagaaaaag agaatgtcca
       61 gcccaaaagt atagctcgtg agatgtttga aaactttagt acgttttcct cttacatcaa
      121 agattatagc cttcaacgtt ctgaagccct tttattacgt cacataaatg gagtttataa
      181 agtcctttcg caaacagtcc ctcctgcttt taaaaatgaa gaactcattg aaatgcaaga
      241 ttatcttcgt gacatgatcc agcgagtgga ttcgagttta ctagaagaat gggaacgcat
      301 gcgtcatcca gaatttaagg cggaagaagt aaaagaatta ggtgcgtttg gagcagagaa
      361 agctcttgaa gacattactt atgataagaa gcgttttttg ggaatggctc gtcaacgtat
      421 ttttgcattg atgacattat tacaaaaaca aaaatatgca gaagttcttg atttattaag
      481 tgaagatctt aatgagggag aacttctcgc agatgcaagt ggtgtacctt ggaccgaaaa
      541 acgattatta gaaaccatgg cgctttatgt ggcagagcat gatcgattcc ttcttgatgt
      601 tgaaggccgt tctttgaaac acactcttgt ggagtattca ggcgatacaa tgcagataca
      661 gcaaatgctt caagatccta acgaattaaa tgattggagt atcgattttg aaattccgct
      721 ttcggcgagt cgagaagctg gtagcgtttt attgcgttta tgccgcattg gtgaagtgac
      781 ttcataaatt cattttgttt ttcataataa aaaccagtat attatacaga aatttaaaaa
      841 aggagaaggt atgcgttcag atgaagtaga ttatgaaatc attggagatg atcttcaaat
      901 tgtagaaata gaattagacc caacggaaac aatcatagcc gaagctggca gtatgaatta
      961 catggaagaa gacatctctt ttgaagcgaa aatgggagat ggttcaaatc caggtgctgg
     1021 catcatggat tcccttctaa atgttggcaa gcgcgtatta acaggggaat ctctttttat
     1081 tactcatttt acaaataagg gttctcaaaa aagaaaagta gcttttgccg ctccttatcc
     1141 agggaaaatt atacccattg accttaatca aattggcggg cgttttattt gtcaaaaaga
     1201 tgctttttta tgtgccgcct atggaacaaa attgagcatc accttaaaca aaagaatagg
     1261 agctggtttc tttggtggag aaggtttcat tttacagaaa ttagaaggcg atggtcttgc
     1321 ttttgtccaa gcagggggaa cgattatcaa aaaagaactt cgtggagaaa cgcttcgagt
     1381 ggacacaggt tgtattgtag gcttttcaga tggcatacaa tatgatattc aaagatcagg
     1441 ctctctcaaa tccatggttt ttggcggaga agggctcttt ttagcgactc ttagaggggt
     1501 tggtactgtt tatttacaaa gcttaccttt ctctcgttta gcagatcgta ttattgctca
     1561 tgctccttct atgggtggtt cttccaaagg agaaggctct gttcttggaa aattagggga
     1621 catatttggt gaccgctaga ggatccattt tctcgagcgt tattttttcc tattttgtct
     1681 ttgaagtgta ttattttgga gttttttaaa tggctgtatt gatgcgtgat atgattctta
     1741 agagtggtgt taattttgga acgagtggcg ccagaggttt agtttcagac atgacagata
     1801 aggtttgtta tgtttatact aaagctttta tccaatactg ttccgctcat tataacgaat
     1861 caaaaaccat tgctgttgca ggggatttgc gcccaagtac agaacgtatt ctctccgctc
     1921 ttgctcaagc cgccaaagaa ttaaataccg aatttgtgta ctgtggccga attcctagtc
     1981 cagcgattgc tctttatggg attgaacaga aaattccaac gattatggtc actggtagcc
     2041 atattccaga tgatcgtaat ggtattaaat ttaatcaccc atttggagaa atatcaaaaa
     2101 aagatgaaga aggtattcta tcgcaaagtg tggatatcga tgaatcgcta tttgatgcat
     2161 cagggatgtt actaaatcct ccgtcattac cctctataga aaccaaggct ataaaacaat
     2221 atactgcgcg ttactcagca ttttttggtc cagaggcttt gatgggtttg actcttggag
     2281 tctatcagca ttccgctgtg gggcgagata ccatagttga agttttagaa tcactaggag
     2341 catttgtaaa gcctttggga cgatccgaaa aatttattcc agtcgataca gaagcgattc
     2401 gaccagaaga tgaagcactt gcaaaaaagt gggctatcga aggtttagat gctattatta
     2461 gtactgatgg cgattcagat cgacctttac ttgcagatga ttcaggagaa tggcttagag
     2521 gagatgtcct tggaatatta gccgcaaagt ctttaggaat caaccgtgtg gcgactcctg
     2581 ttagttgtaa tacagctcta gaaaaatgtg aagcctttga gaaaataagt cggacgagaa
     2641 ttggaagccc ttatgttatc gttggaatgg aaagcctttg tgaaaaggga aaaactgttg
     2701 ctggctatga agctaacgga ggctttttac tcgcaactcc aattgaaaaa gaaggccgta
     2761 ttctaaaagc tcttcctaca agagacgcct tacttcctgt tattgccgtt ttagcagagg
     2821 catgtgcaca tcgtcgaaat gtgtctgatc ttttaaggca tttaccaaag cgttttactt
     2881 caagcgatcg tttaaaagaa tttccaaccg aaagaagtcg gcagattctt gaaaagataa
     2941 agggcaaaaa tcttggcgaa tctttgtttg gaaaattctc aggcaagtta atttctatag
     3001 atgaaaccg
//
LOCUS       DAOOBB010000212         3008 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_36283_length_3008_cov_5.081612, whole genome shotgun sequence.
ACCESSION   DAOOBB010000212 DAOOBB010000000
VERSION     DAOOBB010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36283_length_3008_cov_5.081612"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..1207)
                     /locus_tag="PLT31_08400"
     CDS             complement(62..1207)
                     /locus_tag="PLT31_08400"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPW95188.1"
                     /translation="MKKNDFYFPAIDGLRLLASINIVLLHLSSSSALEYTKNWTWISP
                     IINAPAFAAGIFFVLAGFLFSSKFSDPDRRIPLIPFIFSRIAKLYRLHFLMTLLMFGV
                     LVFKMSSISFAWNAIPEIISDGLSNMVHPFRSLFLHLTLLWSIVPDLGMKLNEPSWAL
                     TSFFLCYAITPFAAKFLFNLSSKKLWILFGVVFVPGIIWGLIYGFSGDLWFADFAAKY
                     RFFHMFAPVRIFEYLFGMILFRLYQTGAFSFLKKKYASGLFQAILLTLLYASLFFLNT
                     SSSAWNYINHHSLPILIYGLLIISLTSGKGLLAYFFCIKPIRAIGRASFYPYLIHLPL
                     ISIAWGLCNLNTPKNTILFLIFIYAISTFYMEFKKRRKKKTNQKTLH"
     gene            complement(1232..1555)
                     /locus_tag="PLT31_08405"
     CDS             complement(1232..1555)
                     /locus_tag="PLT31_08405"
                     /inference="COORDINATES: protein motif:HMM:NF023300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3392 family protein"
                     /protein_id="HPW95189.1"
                     /translation="MLSYIHSFADFLRVHLSSISVGLVATVLTIYGAHINGYFRKITK
                     SLPFIARFALFIVLCSAGYAFLSSQAVKYLRIFLIQQKDIPLILIISGTFLVLAFLAR
                     NGKNL"
     gene            complement(1579..2427)
                     /locus_tag="PLT31_08410"
     CDS             complement(1579..2427)
                     /locus_tag="PLT31_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HPW95190.1"
                     /translation="MKKNEIWYKVQGSTTVDNYELLSYELFEAGISALEELESSDPGK
                     IVDFCFYSDSKEECDRIVSLFPQYQLQISEEAARDWDQWWRDRAQPVSVSPRLWVRPP
                     WVDFTAENPDAVVLELEAKTAFGTGEHDTTASTACLMEDLDFHGKTVLDIGTGTGILA
                     MFARRLGAKLAVGTEIDPLAIPCIAENFERNGFKTSDTVLGFLDAFEDSVQFDVILCN
                     MIRSELWPLRADIEDRLASKGALIISGQLLNEKHYIENWFAEAGFVTKNEKISGEWWS
                     VRAQKT"
BASE COUNT         1068 a          511 c          573 g          856 t
ORIGIN      
        1 ttgttaatgg cgtaaaatat attcttacgg aatccttaga tagagccaaa actgagttag
       61 gttagtggag cgttttttga tttgtttttt tctttcgacg ctttttgaat tccatataaa
      121 acgtactgat cgcatagata aagattaaaa ataggatcgt gtttttaggc gtattaagat
      181 tacataggcc ccaagcgatg cttattaagg gaaggtgtat caagtagggg taaaaagacg
      241 ctcttcctat tgctcgaatc ggtttgatgc aaaagaagta ggcgagtagc ccttttccag
      301 aagtaagcga aataatgagt agcccataaa tgagaatagg aaggctgtgg tgattgatat
      361 aattccaagc agaagaagag gtatttaaga aaaataaact cgcgtagaga agggtgagaa
      421 ggattgcttg aaataaacca ctggcatatt tctttttaag aaaagaaaat gcacctgttt
      481 gatataaacg gaaaagaatc attccaaata aatattcaaa gatccgaaca ggagcaaaca
      541 tgtgaaagaa gcgatatttt gccgcaaaat ctgcaaacca taaatctcca gagaagccat
      601 atataagacc ccaaatgatt ccagggacaa aaacgactcc aaataaaatc caaagctttt
      661 tggaagaaag attaaagaga aattttgctg caaaaggagt gatggcgtag cacaagaaaa
      721 aagaagtgag tgcccaagag ggctcattaa gcttcattcc taaatcggga acgatagacc
      781 ataataaggt gagatgtaaa aataaactcc gaaatggatg aaccatgttg gagagtccat
      841 cggatatgat ttcaggaatg gcgttccaag caaaagagat gctactcatt ttaaagacta
      901 aaacaccaaa catgagaaga gtcattaaaa agtgcaagcg atataatttg gcgattcgag
      961 aaaaaataaa agggattaaa ggtatacggc ggtctgggtc actgaatttc gaagaaaata
     1021 aaaaacccgc taaaacaaaa aagatgccag cggcaaaggc aggtgcatta ataataggag
     1081 atatccacgt ccaatttttg gtatattcta aagcactaga agaagataaa tgtaaaagaa
     1141 caatgttgat ggatgcgagt aatcttaaac catcaatggc agggaaataa aaatcatttt
     1201 ttttcattta tgtttctcat aagcgagtcc attaaagatt ttttccgttt ctcgcaagaa
     1261 aagcaaggac taaaaaagta ccactgatga ttaaaattaa ggggatgtct ttttgttgaa
     1321 tcaaaaagat tcttaggtat ttgaccgctt gcgaacttaa aaaagcatag ccagcactac
     1381 acaaaacaat aaaaagagca aaacgagcta taaaagggag gcttttagtg atttttctaa
     1441 aatacccatt aatatgagct ccgtaaatcg tgagaacggt cgctactaaa ccaacagaaa
     1501 tagagcttaa atgaactctt aaaaaatcag caaaagaatg aatataggat aacatgagaa
     1561 aaagaccctc tttatgaatt aggttttttg agcacgtacg ctccaccact cgccacttat
     1621 tttttcgttt ttagttacaa aaccagcttc agcaaaccag ttttcaatgt aatgcttttc
     1681 gttcaagagt tgcccagaaa taataagagc accttttgat gcaagtcgat cttctatgtc
     1741 ggcacgtaaa ggccacaatt cacttcgaat catattacag agaataacat caaattgaac
     1801 ggaatcctca aaggcatcta aaaagcctaa aacggtatca cttgttttaa atccgttccg
     1861 ttcaaagttt tcagcgatgc aaggaatcgc gagtggatct atttcggtac ccaccgctaa
     1921 cttagcccct aaacgcctag caaacatggc aagaattcct gttcctgtgc caatatctaa
     1981 aactgttttt ccatgaaaat caagatcttc cattaaacaa gcagtacttg ctgtcgtatc
     2041 gtgttcacca gtgccaaaag cggtttttgc ttcgagttca agaacaacgg catctggatt
     2101 ttctgcagta aaatcaaccc aaggagggcg aacccataac cgcggactta cagagactgg
     2161 ttgggctcgg tctcgccacc attgatccca atctctagcg gcttcttcag aaatttgaag
     2221 ttggtattgt ggaaatagag agacgatacg gtcacattct tcttttgaat cagagtaaaa
     2281 gcaaaagtct acgatttttc caggatcact agattcgagt tcttctaatg cagaaatacc
     2341 tgcttcaaaa agttcataac tcaaaagctc gtagttgtct acagtagtgg atccctgtac
     2401 tttataccaa atttcatttt tcttcatgga ctaaatataa aaatactctt ttggtctttt
     2461 ttttaaactg atttgaatca gcgaattatt tcactttatg tgattgattt tgtagattct
     2521 aaagataata tattgatcca ttaattttga caaagaatgc aaattctaaa cttaatgatc
     2581 aagatgaaat gaaacaaaag gctttttttt cagtaaatga agatttttat ggaagtagtt
     2641 ttgctgtgaa ttacaataaa acaaatggag aaaaaaagct ctatttggat acaaatgaat
     2701 cataatgaat ttttatttga tgattcttta ggtggtcttt tcaaatcaat tcacatgcct
     2761 tttgcattta tgaacgttgt catctaatct tcacaagaac ctgcttttat tctatctttg
     2821 acttcgtaaa aacgctcaaa gaataaaaat gcacatgaaa cggtcagttt gtagtattgc
     2881 ttttgcatcg cttcttacca ttttagcctt ggttgaaatc agtagtcgtt tttgcgagca
     2941 aaagaagctc gatgagcaat tagaagaaga tatccaaaaa tgtggtggca aactgaaaga
     3001 aactcgct
//
LOCUS       DAOOBB010000213         2991 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_36572_length_2991_cov_3.559946, whole genome shotgun sequence.
ACCESSION   DAOOBB010000213 DAOOBB010000000
VERSION     DAOOBB010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36572_length_2991_cov_3.559946"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="PLT31_08415"
     CDS             complement(<1..705)
                     /locus_tag="PLT31_08415"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HPW95191.1"
                     /translation="MLSKTILLLKMTRPVNILIAVLTLLAGYCLIDYYPPVMQLFIEC
                     IAFAAAIAFGNIHNDVIDVKVDAINRPDRALQSGKISIPLANSIVGICFGIPVILTLG
                     LLDSTVHATFFALILLILFIYNRWTQNVPLVKNMTVAFLCTTPLILNIINHPKGAVLI
                     APLILFAFFFMFTRELIKDIEDEPGDFRQGILTFPVLVGIRKASIFAIISMIFSLHTL
                     VLPVIIGIYSPLFIVFS"
     gene            731..1834
                     /gene="rsgA"
                     /locus_tag="PLT31_08420"
     CDS             731..1834
                     /gene="rsgA"
                     /locus_tag="PLT31_08420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HPW95192.1"
                     /translation="MIENEQNENKSNPRMKMSRRENRARRLNVKQMWEKGALDDHLVK
                     ERWSREFKNPRLKKIKNPTTHFEPENKLEGLVLEVHRRTCEVRLSDLRVVTAEYKATV
                     VDELGEFPAVGDSVVLGVTSEEKYAVIKVHPRRSALVRPGPRDRLRDELVLAANIDQV
                     VIVASVEQPAFNYGFVDRFLLAANLNRLPFVLVLNKMDLVTEKPKAIVDFMKIVDQSI
                     AISCETKEGLEELKKVLQGKISVFSGQSGVGKSTLINQLVPEASLRTGAVRHKDGKGR
                     HTTTSSSLFDLPGGGMVIDTPGIRSLGLLNIDSNDLAKIFPGFFKDGLFTCKFSNCLH
                     LTEIGCSVREALASGKLSEARYESYLRILNAKE"
     gene            1897..2307
                     /locus_tag="PLT31_08425"
     CDS             1897..2307
                     /locus_tag="PLT31_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95193.1"
                     /translation="MGNFSVYLTIMSSVLFFGYSLSLSTNGYKSICDKAVKFKELLKM
                     EMDASEGIRKTNISLISAFSLAYLVLLCFSGFAYWFLGAVLLKLVSTLLLSDYFQRMV
                     VEDKMISKRLYILMKLDSIFNAVVGLCTPLLIVL"
     gene            2315..2890
                     /locus_tag="PLT31_08430"
     CDS             2315..2890
                     /locus_tag="PLT31_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95194.1"
                     /translation="MNKNLWILLLCLFGWASSQEASDAATTSANTTGNKMPFVLGGVL
                     ETGSGVLANGDHGIGLIRVTPMIGAWINGLGYLRLGYGFNNYVQDPDEGEKREIEQRD
                     FSAQLGISLGFGPYLQGSYTRAKNLSDAGDVAWNEYGLGLGSFFQLGIKSALVFEIEY
                     RFISEHYDPFEKETISGTRLQMNIGFVVYVY"
BASE COUNT          912 a          454 c          672 g          953 t
ORIGIN      
        1 actgaaaaca ataaaaagag gggagtaaat acctattatg acaggtaaga ctaaggtgtg
       61 cagtgaaaaa atcatactta tgatggcgaa gatacttgct tttctgattc caacaagaac
      121 gggaaaagtg agaatgccct gtctaaaatc gcctggttcg tcttcgatgt ctttaatgag
      181 ctctcttgtg aacataaaaa agaaggcaaa tagaattaag ggggcaatca gaacggcacc
      241 tttggggtgg tttataatat tgagaataag aggggtagtg cataaaaaag cgacggtcat
      301 attctttacg aggggtacat tttgagtcca tcggttataa atgaataaaa tcaataaaat
      361 taaggcaaag aaagtggcgt gtacagtgga atctaaaagc cctaaggtga gtatgacagg
      421 gattccaaag catatgccaa caatgctatt tgctaaaggg atagaaatct ttccagactg
      481 caatgcacgg tctggtcggt ttatggcgtc tacttttaca tcaataacgt cgttatggat
      541 atttccaaaa gcaatagcag cagcaaaagc gatgcattct ataaaaagct gcattacagg
      601 gggataatag tctattaaac aatatccagc gagtaaagtc aatactgcta ttaaaatatt
      661 gactggtcta gtcattttta gaagaagaat tgtttttgaa agcatatatg taaaactaca
      721 tttgtattta atgattgaaa acgaacaaaa tgaaaataaa tccaatccac gcatgaaaat
      781 gtcgaggaga gaaaatagag ctcgccgttt gaatgtaaaa caaatgtggg aaaagggtgc
      841 tttggatgat catttagtta aagagcgctg gagcagagag tttaagaacc cacgcttaaa
      901 gaaaattaaa aacccaacga ctcattttga accagaaaac aaactggaag gccttgtttt
      961 agaagttcat cgacggactt gcgaggttcg tttatctgat cttcgggtgg taactgcaga
     1021 gtataaagcg acggtggtag atgaacttgg agagtttcct gcagtaggag attcagtcgt
     1081 tttaggtgtt acttcagaag aaaaatatgc cgtgattaaa gtgcatccta gacgaagtgc
     1141 tttagtgcgt ccagggccta gagatcggct gcgcgatgaa cttgtacttg ccgccaatat
     1201 tgaccaagtg gtgattgttg cttctgtaga acaacctgct tttaattatg gctttgtgga
     1261 tcgcttttta ctcgctgcta atttgaatcg tttgcccttt gttttggttt tgaataaaat
     1321 ggatctagtg actgagaaac caaaagccat tgtggatttt atgaaaatcg tggatcaatc
     1381 tattgcgatt agctgtgaaa ccaaagaggg cttagaggaa ttaaagaagg ttcttcaggg
     1441 aaaaatttct gtatttagcg gccagagtgg tgtcggaaaa tccaccttga tcaatcaatt
     1501 ggttccagaa gcttctttaa gaactggtgc agttcgtcat aaagatggaa aaggaaggca
     1561 tacaaccaca tcctctagtt tatttgattt gcctgggggt ggtatggtga ttgatacccc
     1621 agggattcgt tctttgggac ttttgaatat tgattcgaat gacttagcca agatctttcc
     1681 aggctttttt aaggacggtt tattcacctg taaatttagt aactgcttgc atctcacaga
     1741 gataggatgc tctgtcagag aagctttagc ttctgggaaa ctttcggagg caaggtatga
     1801 aagctattta cgtattttaa atgctaagga gtagctcttc aaatcaattt gtagattaaa
     1861 tttaataggt gtgctttttg aggatacttt aatataatgg gaaatttttc agtttattta
     1921 acaatcatgt catccgttct ttttttcgga tatagtttgt ctttaagtac aaatggttat
     1981 aaatcgatct gtgataaggc cgttaaattc aaagaactat taaaaatgga aatggatgct
     2041 tctgaaggga tcagaaaaac aaatatttca ttaatatctg cgttttcttt agcttattta
     2101 gtacttctat gtttttcagg ttttgcctat tggtttttag gagctgttct tcttaaatta
     2161 gtctcgactc ttcttttatc agactatttc cagcgtatgg ttgttgaaga taaaatgatt
     2221 tcaaaacgtt tatatatact catgaaatta gattctattt ttaacgcggt tgttggcttg
     2281 tgtactcctt tattgattgt gctttaggaa aaaaatgaat aaaaatctat ggattctgtt
     2341 attgtgcctt tttggatggg cttcttcaca agaggcttcc gatgctgcaa caacctctgc
     2401 aaatacgaca gggaacaaaa tgccttttgt gcttggtggc gttttagaaa cagggtctgg
     2461 agttttagct aatggagatc atggtatcgg gcttatccgt gtcacgccga tgattggtgc
     2521 ttggattaat gggcttggtt atttgcgttt agggtatggt tttaataatt atgtgcaaga
     2581 tcccgatgaa ggagaaaaaa gagaaatcga acagcgtgat ttttctgctc aattagggat
     2641 ttccttgggc tttggtccat atcttcaagg atcatacaca agagcaaaaa acttgtcgga
     2701 tgctggagat gtcgcatgga acgaatatgg cttgggatta ggttctttct ttcagttagg
     2761 gattaagtcg gcattagttt ttgaaattga atatcgtttt atttcggaac attatgatcc
     2821 ctttgaaaaa gaaacgattt ctggaacacg tcttcaaatg aatataggtt ttgttgtcta
     2881 tgtctattaa aacggccgct attttagggg gggcctttga tccagtacat aatgatcatt
     2941 tagcgttagc atctacatgt ttatccttaa aactttgcga tgaagtatgg t
//
LOCUS       DAOOBB010000214         2981 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_36723_length_2981_cov_3.970950, whole genome shotgun sequence.
ACCESSION   DAOOBB010000214 DAOOBB010000000
VERSION     DAOOBB010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36723_length_2981_cov_3.970950"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1344)
                     /locus_tag="PLT31_08435"
     CDS             complement(<1..1344)
                     /locus_tag="PLT31_08435"
                     /inference="COORDINATES: protein motif:HMM:NF023358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3450 family protein"
                     /protein_id="HPW95195.1"
                     /translation="MKNILLILFFSLSASFVYAAPPSGEQLVKERAQLNSAKADLEEA
                     RKKRDMAVAARWKDREIANKERELFNEKYREQKDKIDALMSERARLLEDVRVAREDLS
                     QVKAAGERSRSDFLALALTPDVLDGLTQLQEQGIPFKMTDRIENMNRLKKEIDLYRDD
                     PMRLVRSVFSVLEYELIFSREVELDTGDLIFDNSVKRGQRLRLGGLFAMQKSDSDSSV
                     ALLLPVAGEKGSSFSWQTNLIPGVQTSIYSAFASVNDKEEFLIPVDPLLSTTLSSELA
                     SQNEKTFKENAIQFFKDGGILMYPIFALLILSILLIIERFIVISFKSYSKGTHDVLKA
                     LESNSIDEARRLSVKVKGSIGSILRRGLEKDYENREAAEKAIEEVFAAETPSIEKGLS
                     SISVMASTAPLLGLLGTVMGMIELFEVITMHGTSDPKLLAGGISIALITTQAGLMV"
     gene            complement(1341..2123)
                     /locus_tag="PLT31_08440"
     CDS             complement(1341..2123)
                     /locus_tag="PLT31_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3450 family protein"
                     /protein_id="HPW95196.1"
                     /translation="MKKLFFICVMAISYTMGQESVESVRRQIKAVQAETAREVNLHNE
                     EKKRHSDFIEVGRKKVVALNSQNQSLKAEMDSMKVELSRLKEARQKALSSSRFYENKK
                     IKYQEALALAIDSLQHLFQTDFPYKNEDAVNSVSEIAAQLKKGHISSSDALARTVEVF
                     LERIRMGYTTEVWKGSLPLSTRTVHGTYMRYGAVASIFVSLDGQEVFWLTRSPENNYS
                     WKEASQDMEMRTIIKEALKVAEGKTTPKIVPIPVYLKRGELK"
     gene            complement(2193..2930)
                     /locus_tag="PLT31_08445"
     CDS             complement(2193..2930)
                     /locus_tag="PLT31_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPW95197.1"
                     /translation="MKKLSLYLFVAVFVMLFAGCNSKMDPNDPQSVRKEIAKQKIAFT
                     PNQFVSYAAAGDIAKMTLFLQAAFEIDQPDNNGNNAVALVVNQGKTDVLKFLFEKGAK
                     ANVFNSKGEHVVDNAVVMGHYEVLQVLIDQLKKEEVELVNITSAVIIATKTGNTQALQ
                     ILGEAGVPLHTRGADGYYPIHLAVKGGHYDAMMYLISKGVDLNSKCNQGYSVLDWAKN
                     EGYSRLISALKKAGAKHTPAYIKEFGR"
BASE COUNT          960 a          620 c          460 g          941 t
ORIGIN      
        1 aaccatcaaa cctgcctgag tcgtgattaa agcaatagaa ataccaccag ctagtagttt
       61 aggatcagag gtgccatgca ttgtaatcac ttcaaaaagt tcgatcattc ccatcacagt
      121 tcctaaaagt ccaagtaaag gagctgttga agccattact gaaatagagc ttaacccttt
      181 ttctattgaa ggagtttcag ctgcaaagac ttcttcaatg gctttttcag ctgcttccct
      241 attttcataa tctttttcta aacctcttct taaaatagaa ccaatagagc cctttacttt
      301 tacagacaga cggcgagctt catcaattga atttgattct aaagccttca aaacatcgtg
      361 agttccttta gaataggact tgaaagagat cacaataaag cgctctataa ttaaaagaat
      421 agacaaaata agaagagcaa aaatagggta cattaaaata ccaccatcct taaaaaattg
      481 aattgcattt tccttaaatg tcttttcatt ttgacttgca agttcagaag aaagtgttgt
      541 gctcaataaa ggatctacag gaattaagaa ttcttcttta tcattcactg atgcaaaagc
      601 agaataaatc gaagtctgaa ctccaggtat taaattcgtc tgccaagaga acgagctacc
      661 cttttctcca gccacaggaa gtaatagtgc aacagacgaa tcggaatcac ttttttgcat
      721 cgcaaaaagc ccgcccaagc gcaatcgttg ccctctcttc acactattat caaaaatcaa
      781 atctcctgta tcgagttcca cttctctaga gaaaattaat tcgtattcta aaacagaaaa
      841 aacgctacga actaaacgca ttggatcatc tcgatacaaa tcgatttcct tttttagacg
      901 attcatattc tcaattcgat ccgtcatttt aaatggaatg ccttgctctt gaagttgagt
      961 taaaccatcc aatacatcag gagtcaatgc taaagctaaa aaatcggaac gacttctttc
     1021 acctgccgct ttgacttgag ataaatcttc tcgggcgact cgcacgtctt caagtaaacg
     1081 agcgcgttct gacattaagg catcaatctt atctttttgc tctctgtatt tttcgttgaa
     1141 tagttctctt tctttatttg ctatttcgcg gtctttccat ctagccgcta cagccatgtc
     1201 tctctttttt ctcgcttctt ctaaatccgc ttttgcacta ttaagttgag ctcgttcttt
     1261 tactaattgt tctccgcttg gaggagcagc ataaacaaag gaagcactta ggctaaaaaa
     1321 taaaatcaaa aggatgtttt tcatttaagc tcacctcttt tcaaataaac tggaattggg
     1381 acaatcttag gagttgtttt cccttcagcc actttaagcg cttcttttat tattgtacgc
     1441 atttccatat cctgagaagc ttctttccat gaataattat tttctggaga acgagttaac
     1501 cagaaaactt cttgcccatc taaacttaca aaaatagaag caacggctcc ataacgcata
     1561 taagtaccat gaacagttct cgtactcaaa ggtaaagagc ctttccatac ttcagtggta
     1621 tatcccattc gaatacgttc taaaaagacc tctactgtac gagcaagagc atcagaagaa
     1681 gaaatatgtc ctttttttaa ctgagcagca atctcagaaa cgctattcac ggcgtcttca
     1741 tttttataag gaaaatctgt ttggaataga tgttgaagag aatcaatggc taaagcaaga
     1801 gcctcttggt attttatttt tttattctcg taaaaccgag aagaagaaag cgctttttga
     1861 cgagcctctt ttaatcgaga aagctctacc ttcatcgaat ccatttcagc tttaagagat
     1921 tgattttgag aattcaaagc gactactttt tttcgcccta cttcaataaa atcagaatga
     1981 cgtttttttt cttcattatg aagattcact tctctcgccg tctcagcttg tacagcctta
     2041 atttgccgac gcacactttc taccgattct tgccccatag tataagaaat cgccattaca
     2101 caaataaaaa aaagtttttt catattacaa aaatacaaag aacctctagg aaaaacctag
     2161 aggttcttta agaaaactat taaaaattag gattagcggc caaattcctt aatatatgca
     2221 ggagtatgct tagctcctgc ttttttcaaa gctgaaatta agcgagaata accttcattt
     2281 ttagcccagt ctaatactga gtatccctga ttgcatttgg aattcaagtc cacaccttta
     2341 ctaatcaaat acatcatggc atcataatgg ccacctttaa cagccaagtg aatcggataa
     2401 taaccatcag caccacgagt atgaagagga acgcctgctt caccaagaat ttgaagagct
     2461 tgagtgttac ccgtttttgt agcaataatg acagctgatg taatattgac taattccact
     2521 tcttctttct ttaattgatc aatcaacact tgtaaaactt catagtgccc catgacaaca
     2581 gcattatcta caacatgctc tcccttacta ttgaaaacat ttgctttagc acctttttca
     2641 aagagaaatt ttagaacatc tgtttttcct tgatttacaa ctaaggcaac agcattatta
     2701 ccattattat caggttgatc aatttcaaaa gcagcctgta agaaaagggt cattttcgca
     2761 atatcgccag cggcagcata agaaacaaat tgatttggag taaatgcaat tttctgtttt
     2821 gcaatttctt ttcttactga ttgaggatca ttagggtcca ttttactgtt acaacctgca
     2881 aaaagcatca caaaaacagc tacaaataag tacaaagaaa gcttcttcat tgattatctc
     2941 caaaaaataa ttgttttaac ttgtagaaaa atatactttt t
//
LOCUS       DAOOBB010000215         2972 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_36868_length_2972_cov_4.635927, whole genome shotgun sequence.
ACCESSION   DAOOBB010000215 DAOOBB010000000
VERSION     DAOOBB010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36868_length_2972_cov_4.635927"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(494..1474)
                     /gene="dusA"
                     /locus_tag="PLT31_08450"
     CDS             complement(494..1474)
                     /gene="dusA"
                     /locus_tag="PLT31_08450"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: protein motif:HMM:NF008774.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HPW95198.1"
                     /translation="MDLNFNRRLSIAPMLDYTDRHERYFLRLLSKHILLYTEMVTTHA
                     LLHAGADRFLKHDPKEHPVALQLGGNDPSALAECSKMGEDAGFSEINLNCGCPSDRVQ
                     SGAFGACLMKEKNIVAECISAMQKAVSIPVSIKTRIGVDHDDSWEFFFDFVKTIADTG
                     CNTFIIHARKAWLKGLSPKENRTKPPLMYDFVFRLKEELPHLNISLNGGVQSLNTVEE
                     CLKIVDGVMVGREAYENPWFLAEADERIFGEKSVAPPNQKALLEAFLPYMEQQLKEGC
                     PLTMMTRHLFGLFNGIPGARRYRQIISEEANRRGAGIETVIKAINAVSEI"
     gene            1509..2405
                     /locus_tag="PLT31_08455"
     CDS             1509..2405
                     /locus_tag="PLT31_08455"
                     /EC_number="3.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartoacylase"
                     /protein_id="HPW95199.1"
                     /translation="MDKIKTVIISGGTHGNELTGVQLIKKWTTNPELCKNKVPDVQVD
                     LLMVNPAAVAIVRRYVDHDLNRSFANHMLTGSTQSLCVEWNRAQELNGTYGPKGDSTK
                     TDLILDIHNTESNMGICLILSTKDPFTTRASAQIAHEFPNVHIYYQPEERGASPYFGT
                     LARADVCIEVGPQSHGTLQASLFEQTEQVVYRYLELISEWNNQVLQKKEKKKVTVFTQ
                     HRDVDYPRDSNGNISAMIHPSLHSSDYLELKPGAPLFRTFDGKDIPFENDHSVWPLFI
                     NEAAYYEKKIAMSFTLKSIEEW"
     gene            2405..>2972
                     /locus_tag="PLT31_08460"
     CDS             2405..>2972
                     /locus_tag="PLT31_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95200.1"
                     /translation="MGKNELEKRMQSFLSTLQEQKALGWESRFHEILDAFEDFLINRP
                     EPPEEWKARLGADVKKYDYYQIVLPADFEDPYEEDLGNIHRLRAEFEAVPVTMAIEHL
                     LISRNYFIFENGHADPIPAPRPLLMLESVDDEGSKIDWDCCITVFSDGSFYAYNIRND
                     QEEVLGEDIKAILEDQMDVLCEMQLVIPI"
BASE COUNT          914 a          517 c          618 g          923 t
ORIGIN      
        1 acgcttggtt cttcataaaa aaactcggtt tgtttagatg agcttttctt tttttcagaa
       61 gagctcatca ataacaacca tttccgcctg ctaattagct agtgtttgtt aattgaatac
      121 catttttctt catttgaatc tacgctattg aatttacaat aacacgtaca cttgcacttt
      181 tacgtgaaac ttagcggttt accgctgtag ttgaaccatg cagagctgcg cttagtaaga
      241 aacatgaaca cttagtagtt tactacttac gtgagagtga tacccttgtg gtagtgatac
      301 ccttgtggta gtgataccct ttgggtaatg atacccttgt ggtaaacatg aacgcttgac
      361 tcttcatgaa aaaaactggg ttgggttaga ggggcttttt agtgttttgt tgaaaaactt
      421 aaacaagtaa gtttcgcgaa aatcaatcta tgcgaatggg aaattgttct tcacttgcac
      481 caaataaaca catctaaatt tcagaaacgg cgttgatggc tttaatcacg gtttcgattc
      541 ctgcgcctct tcgatttgct tcttcgctga tgatctgtct atatcttcta gcgccaggga
      601 tgccgttaaa aagaccaaac agatgtcttg tcatcatggt taagggacaa ccttctttca
      661 actgctgttc catgtaaggt aaaaaagctt caagtagtgc tttttgattt ggaggagcga
      721 ctgacttttc tccaaaaatg cgttcgtcgg cttctgctaa aaaccaagga ttttcgtaag
      781 cttctctacc gaccatcacc ccgtctacta ttttaagaca ttcttctacg gtattgagag
      841 attgaacccc gccattcaga gaaatattta aatggggaag ttcttctttc aaacgaaaaa
      901 caaaatcgta cattagtggg ggttttgtgc ggttttcctt gggacttaat cctttaagcc
      961 aagcttttct agcatgaata ataaaggtgt tacaacctgt atctgcaatt gtttttacaa
     1021 agtcaaagaa gaattcccaa gaatcatcat gatctacgcc aatacgagtt ttaattgaaa
     1081 cagggattga aacggctttt tgcattgcag aaatacactc ggcgacaata tttttttctt
     1141 tcatgagaca ggcgccaaat gcaccacttt ggactcgatc cgaaggacaa ccgcaattga
     1201 ggttaatttc agaaaagcca gcgtcctcac ccattttaga acactctgca agtgcagaag
     1261 ggtcatttcc gcccaattgt aaggccacgg ggtgctcttt tggatcatgt tttagaaaac
     1321 gatcagcccc agcatgaaga agggcgtggg tagtcaccat ttctgtatat aaaaggatgt
     1381 gtttgcttaa taaacgcaaa aaataacgct catgacgatc cgtatagtcg agcataggag
     1441 cgatagaaag tcttctatta aaattcaaat ccataaggta aagttagttt ttctaccttt
     1501 cttttagtat ggataagata aaaacagtga ttatctctgg tggaactcat ggtaatgagt
     1561 taactggagt tcaattaata aaaaaatgga ccactaatcc agagctttgt aaaaataaag
     1621 ttcctgatgt tcaagtagat ttattaatgg ttaatccagc ggcagtagcg atagtaaggc
     1681 gctatgtaga tcatgatctc aatagaagct ttgctaatca tatgttaaca ggatcgactc
     1741 agtctctttg tgtagaatgg aaccgagctc aggaattgaa tggaacttat ggcccaaaag
     1801 gcgattctac aaaaacggat ttgattttag atattcataa tacagaatct aatatgggga
     1861 tttgcctcat tttgtctaca aaggatccct ttacaacaag agcttctgct caaattgctc
     1921 atgaatttcc taatgtgcat atttattatc agccagagga acgaggggca tcaccttatt
     1981 ttgggacttt agctcgtgcg gatgtttgta tagaagtggg gcctcaaagt catggtactt
     2041 tacaggcaag tctatttgaa caaacagaac aagtggttta tcgttatctt gaacttattt
     2101 ctgaatggaa taatcaagta ttacagaaaa aagaaaaaaa gaaagttacg gtatttacac
     2161 agcatagaga cgtggattat ccaagggact ctaatgggaa catcagtgcg atgattcatc
     2221 ctagtcttca tagttcagat tatttagaac taaagccagg agcacctttg tttagaacgt
     2281 ttgatgggaa agatattccc tttgaaaatg atcatagtgt ttggccgtta tttattaatg
     2341 aagcggctta ttatgaaaag aaaatagcga tgagctttac attaaaatca attgaggaat
     2401 ggtaatggga aaaaatgaat tagaaaaaag aatgcaatct tttttaagta ctctccaaga
     2461 acaaaaagct cttggatggg aatcccgttt ccatgaaatt ttagatgctt tcgaagactt
     2521 tttgattaac aggccagaac cgccagaaga gtggaaagct cgtttgggtg cagatgtcaa
     2581 aaagtatgat tattaccaga ttgtccttcc tgcagatttt gaagaccctt acgaagaaga
     2641 tttaggcaat attcaccgtt tgagggcaga atttgaagct gttccagtga caatggctat
     2701 agagcatttg cttattagca gaaattactt tatttttgag aatggacatg ctgatccaat
     2761 acctgctcct cgtccgttgt taatgcttga gagcgtagat gatgaaggtt cgaaaataga
     2821 ttgggattgt tgtatcacag ttttttcgga tgggagtttt tacgcttata acattcgtaa
     2881 cgatcaggaa gaggttttgg gagaggatat taaggcgatt cttgaagatc aaatggatgt
     2941 cctttgtgaa atgcaattag tgattccaat tg
//
LOCUS       DAOOBB010000216         2960 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_37052_length_2960_cov_3.647504, whole genome shotgun sequence.
ACCESSION   DAOOBB010000216 DAOOBB010000000
VERSION     DAOOBB010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37052_length_2960_cov_3.647504"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2080
                     /gene="ppdK"
                     /locus_tag="PLT31_08465"
     CDS             <1..2080
                     /gene="ppdK"
                     /locus_tag="PLT31_08465"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HPW95201.1"
                     /translation="AEYKKKIGSDFPSDPKEQLMGAIKAVFRSWNNPRAIYYRRMNDI
                     PGSWGTAVNVQAMVFGNMGDTSGTGVAFTRNPSTGERKLYGEFLMNAQGEDVVAGVRT
                     PQSIDHLKEIMPECYDQFVEISNKLEKHYRDMQDMEFTIENKKLYMLQTRNGKRTAAA
                     ALKIACDLVDEGMINEKEAVCMIDPKQLDALLHPQFDVIALKKAKPIAMALAASPGAA
                     CGKIVFTAEDATEWATKRNEKVILVRLETSPEDIEGMHYSEGILTVRGGMTSHAAVVA
                     RGMGTCCISGCGEIKMDLENKCFTLGGRTFKEGEYISLDGSSGKIYGEAIPTVEASIS
                     AELGRLMGWADQYRVLKVRTNADTPKDAKQAVLFGAEGIGLCRTEHMFFEGDRIKATR
                     EMICSDTVEGRTKALAKLEPMQQGDFEELYEALGGNPVTIRFLDPPLHEFVPTVKEDI
                     EELAKTMGKTVNDIENIIASLHEFNPMMGHRGCRLAVTYPEIAAMQTRAVIKAALKVK
                     TKHPDWNLFTEIMIPLIGDLKEFSYVKSIVVKAADELIKNSNINLEYKVGTMIEIPRA
                     ALTADEIAEEADFFSFGTNDLTQMVFGFSRDDAGKFLDAYYTNKIYESDPFARLDIPG
                     VGKIVKFAVEAGRRVRPNIKLGICGEHGGDPSTVAFCHEIGLDYVSCSPFRVPIAKLA
                     AAQAAIRQKK"
     gene            complement(2272..>2960)
                     /locus_tag="PLT31_08470"
     CDS             complement(2272..>2960)
                     /locus_tag="PLT31_08470"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HPW95202.1"
                     /translation="LDNIFVMAAIFSFFKVPKKYQHRVLFWGILGAIVLRGIFIFAGT
                     ALIVKFSWLMYVFGVLLIVSAIKMGISKEDHDEDLSKNFAVKIAKKFYRVTPTFHGHA
                     FTVIIDGVRHITPLLLALIVIETSDVMFAVDSIPAVFSVTLDPFIVFTSNIMAILGLR
                     SLYFALSAMLGKFVYLKYALVFILAFVGVKMIIVHYVHIPTLLSLSVILLSITISILA
                     SFLKGKEIEQ"
BASE COUNT          969 a          574 c          620 g          797 t
ORIGIN      
        1 ggcagaatac aagaaaaaaa ttggaagcga ttttccttct gacccaaaag aacaattgat
       61 gggtgctatt aaagctgtgt tccgttcttg gaataaccca cgcgctatct attatcgtcg
      121 catgaacgat attcctggtt cttggggaac cgcggtaaac gtgcaagcga tggtatttgg
      181 taatatgggt gatacttctg gtacaggcgt tgcctttact cgtaaccctt ctactggcga
      241 acgcaaatta tatggcgaat ttttgatgaa cgctcaaggg gaagatgtgg tggctggggt
      301 acgcactcca cagagtatcg atcatcttaa agaaattatg cctgaatgct acgatcaatt
      361 tgtggaaatt tcaaacaaat tagagaaaca ttaccgcgac atgcaagata tggagtttac
      421 tattgaaaat aaaaaactct atatgctaca aacacgtaac ggtaaacgca ccgcagctgc
      481 agctcttaaa attgcttgcg atttagtaga tgaagggatg attaatgaga aagaagctgt
      541 ttgcatgatc gatccaaaac agctcgacgc tcttttacac ccacagtttg atgtaattgc
      601 acttaagaag gcaaaaccaa ttgctatggc tcttgcagcg tctcctggag ctgcttgtgg
      661 aaagattgtt tttaccgctg aagatgccac tgaatgggcc accaaaagaa atgagaaagt
      721 cattcttgtt cgtttggaaa catctcctga agacattgaa ggaatgcatt attcagaagg
      781 tattttaact gttcgtggtg gcatgacatc gcacgctgct gtcgttgctc gcggcatggg
      841 cacttgctgt atctcgggtt gtggcgaaat taaaatggat ttagaaaaca agtgttttac
      901 acttggaggc cgcaccttca aagaaggcga atatatttcc cttgacggtt cttctggtaa
      961 aatttacgga gaagcaatcc ctacagtgga agcctctatt agtgctgaac ttggtcgttt
     1021 gatgggctgg gcagatcaat accgcgtact taaagtccgt accaatgctg ataccccaaa
     1081 agatgccaaa caagcggtct tgtttggtgc agaaggtatt gggctttgcc gcacagaaca
     1141 catgttcttt gaaggcgacc gtattaaagc gactcgcgaa atgatctgct ctgacactgt
     1201 ggaagggcgt acaaaggctc ttgcaaaatt agaaccaatg cagcaaggcg attttgagga
     1261 gttatacgaa gccctcggcg gaaatcctgt gacgattcgt ttcttagatc ctcctcttca
     1321 tgaatttgtg ccaacagtaa aagaagatat cgaagagcta gccaaaacca tgggaaaaac
     1381 cgtaaacgat atcgaaaaca tcattgcttc gcttcacgaa tttaacccta tgatgggcca
     1441 cagaggttgt agactagcgg taacataccc agaaattgca gccatgcaaa cacgtgccgt
     1501 aattaaagcg gctttgaaag taaaaacaaa acacccagat tggaatcttt tcacagaaat
     1561 catgattcct cttattggcg acttaaaaga attcagctat gtgaaatcca ttgtagttaa
     1621 agccgcagac gagttgataa agaattcaaa cattaatctc gaatataaag tgggaacgat
     1681 gattgaaatt ccaagagccg ctctcactgc tgatgaaatt gcagaagaag ccgatttctt
     1741 ctcgtttgga acaaatgacc ttactcagat ggtgtttgga ttctctcgtg atgatgcagg
     1801 caaattcctt gacgcgtatt acaccaataa aatctatgaa tcagatccat ttgcacgctt
     1861 ggatattcct ggtgtaggta agattgtgaa attcgcagtg gaagcaggtc gtcgtgtacg
     1921 tccaaatatt aaactcggta tttgtggtga acatggtggc gatccatcta cggtcgcttt
     1981 ctgtcacgaa attggtcttg actatgtgtc ttgttcccca ttccgtgttc ctattgcaaa
     2041 gcttgctgca gcacaagctg ccattcgtca aaagaagtaa gctcttaaaa tagtgtataa
     2101 aaaataaccc cgaaagtgga attcgacttt cggggttttt atttataata agcaaaaaaa
     2161 aatgaatacc aaatttcaaa aatagtcgaa gataaacgta gaaggtagcc attgtacccg
     2221 tgaccacgaa ttcaggaata aaaagaatgg ctaaaagcta tacctagata attattgttc
     2281 tatttctttg ccttttagga agcttgctaa aatgcttatc gtgatggata gcagaatcac
     2341 cgaaagactt aataatgttg gaatatgaac ataatgtacg ataatcattt tcacgcccac
     2401 aaaagcgaga atgaaaacta aggcgtattt taagtagaca aattttccta acatagccga
     2461 tagcgcaaag tataaagacc ttaagcctaa aatagccatg atattagaag taaagacaat
     2521 aaacggatct aacgtcacag agaatacggc aggaatacta tctacagcaa acatgacatc
     2581 cgaagtttct ataacaataa gtgcgagtaa taaaggggta atatgacgaa caccgtctat
     2641 tattacggta aatgcatgcc catgaaaagt aggagtcaca cggtaaaatt ttttagcgat
     2701 ttttacggca aagtttttag ataaatcctc atcatgatct tctttactaa tacccatttt
     2761 aattgcactt acaataagta aaacgccaaa aacatacatc aaccaagaga acttgacaat
     2821 taacgctgta cctgcaaaaa taaagattcc gcgtaaaaca atcgctccca aaatacccca
     2881 aaacaagact cgatgctggt attttttagg aactttaaaa aagctgaaaa tggccgccat
     2941 cacaaaaata ttatctaatg
//
LOCUS       DAOOBB010000217         2946 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_37274_length_2946_cov_3.434106, whole genome shotgun sequence.
ACCESSION   DAOOBB010000217 DAOOBB010000000
VERSION     DAOOBB010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37274_length_2946_cov_3.434106"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /gene="glgB"
                     /locus_tag="PLT31_08475"
     CDS             <1..1157
                     /gene="glgB"
                     /locus_tag="PLT31_08475"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HPW95203.1"
                     /translation="PKDAHALGRFDGTACYEHADPRQGEHPHWGTFIFNLARKEVSNF
                     LISNAMYWLREFHCDGLRVDAVASMLYLDYGKENGGWVPNKDGGNINYETMEFLKHLN
                     SIMGKLTPHAILIAEESTSFPCITRPPERGGLGFHYKWNMGWMNDFLSYIEHEPVHRK
                     YHHNKLTFSMIYAYSENFIQVFSHDEVVHGKGSMLAKMPGDHWQKFANLRLTYAFMYA
                     HPGKKLNFMGNDFGQFREWNEKLSLDWHLLSWEPHGKLHGMVRILNDVYKKESAFWEV
                     DHHQDGFEWIWCDDADNSIVSFVRKDARGNHILCVFNFTPVPRAPYRIGTSARGVWKE
                     IFNSDASLFGGSNLGNLGQVSTQDIPWQNRPWSIEIQLPPLAAVYFKLER"
     gene            complement(1245..>2946)
                     /locus_tag="PLT31_08480"
     CDS             complement(1245..>2946)
                     /locus_tag="PLT31_08480"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPW95204.1"
                     /translation="LPMDSSPIVVKNKVQELSITAWETYIRTLRPMASSWIRTAIRVG
                     SAPSIFSPDGNHFSGTSLAAAGLTFGKMIDKKTPGEKRIAILVPPTAPGIIANLACLI
                     KAKTVINLNYTSPVMTMKYCCDQGGVKTIITAKAFVAKLTERGLDLTPLFESYNILYM
                     EDLKAELSKVSLVRNFIRARLLPAWWIEFWDFKKVSMDDIASILFSSGSEGNPKGVEL
                     THFNLMGNIKQCMSVLNPGSDDVMLGVLPLFHAFGFSITSMMCIVEGVPVVAYPDPTD
                     AKMIGRICAEFKVTIMVGTGTFLRMWGTNRYVHPLMFASVRAVWAGAEKIREELRTLY
                     RTKFQKEIYEGFGTTETTPVAAVNTLDTLLEDYQTVQQGNKPGTVGAPLPGTQFRIVD
                     PDTMEELPIGEDGLILIGGAQIMRGHLNDPERTAQVITIIDGRRWYKTGDKGHVDQDG
                     FLTIVDRYSRFAKLGGEMVSLGSIEFKISESMVLDGIDFCTVSVPDPVKGESIVLLFA
                     GEEATEELVREKVKSVKLPPLMQPSFYIKLDVLPKLGSGKSDFIKSKAVAIEKLASKK
                     "
BASE COUNT          904 a          594 c          546 g          902 t
ORIGIN      
        1 ttcctaaaga cgcccatgcg ttaggtcgat ttgatggaac cgcttgttat gaacatgcag
       61 acccacgtca aggagaacat cctcattggg gaactttcat tttcaactta gctcgtaaag
      121 aagtctctaa ctttttgatc tcaaacgcca tgtactggtt gcgtgaattc cactgtgacg
      181 gtcttcgtgt cgatgctgta gccagtatgt tataccttga ttacggtaaa gagaatggcg
      241 gctgggttcc aaataaagat ggtggtaaca tcaactatga gacgatggaa tttctaaagc
      301 atttgaatag tattatgggt aaattaacac ctcatgccat tcttattgca gaagaatcca
      361 caagctttcc gtgcattaca aggcctccag aacgaggtgg acttggcttc cattataagt
      421 ggaacatggg atggatgaat gactttttaa gttatataga acacgaacct gttcatcgca
      481 aataccatca taacaaactc acgtttagta tgatttatgc ttattctgaa aactttattc
      541 aagtctttag tcatgatgaa gtcgttcatg gcaaagggag catgcttgct aaaatgcctg
      601 gcgatcactg gcaaaaattc gctaatttgc gtttaacata cgcctttatg tacgctcatc
      661 cagggaaaaa actcaatttt atgggcaatg attttgggca attccgtgaa tggaatgaga
      721 aactatctct tgattggcat ttattgagct gggaacctca tggtaagtta catggaatgg
      781 ttcgtatttt aaatgatgtc tataaaaaag aatctgcatt ttgggaagtc gatcatcatc
      841 aagatggttt tgaatggatc tggtgtgacg atgcggataa ttccattgta agctttgttc
      901 gaaaagatgc acgaggcaat catattttat gtgtatttaa cttcaccccc gttccaagag
      961 ctccatatcg tattggaacc tctgcaagag gagtttggaa agaaattttc aattctgatg
     1021 ctagtttatt tggcggaagc aatcttggta atttgggtca agtttccaca caagatatcc
     1081 cttggcaaaa tagaccttgg tctattgaga tacaattacc tcctctcgct gccgtttact
     1141 ttaagttaga acgttaagtt ttaggcctag agcctaactt aacatttttg aataaataca
     1201 aaaaatgccg ccaatgattt ggcggcattt tgttttataa ctgtttattt ttttgaagca
     1261 agtttttcta tagccactgc ttttgatttt ataaaatcag acttgccaga gcctaatttt
     1321 ggaagaacgt cgagtttaat ataaaaagaa ggttgcatta atggaggtaa tttcacagac
     1381 ttcacctttt ctctgacaag ctcttctgta gcttcttctc cagcaaaaag aagaactata
     1441 ctttcaccct tgactggatc tggcacagag acagtacaaa aatctatgcc atctaatacc
     1501 atcgattcag agattttaaa ctcaatggaa cctaagctaa ccatttctcc accgagtttt
     1561 gcaaaacggc tatagcggtc aacaatagtt aaaaaaccat cttgatccac atgcccttta
     1621 tctcctgttt tataccagcg acgcccatca ataatagtga ttacttgtgc tgttctttca
     1681 ggatcattta agtggcctct cataatttga gcgccaccaa ttaaaattaa tccatcttca
     1741 ccaataggaa gttcttccat tgtatctgga tctacgattc taaattgagt accaggtaaa
     1801 ggagctccta cagtccctgg tttatttccc tgttgaactg tttgataatc ttctaaaaga
     1861 gtatctaaag tatttactgc agccacaggc gttgtttctg tagtaccaaa gccctcataa
     1921 atttcttttt ggaacttagt acgataaagc gtacgcaatt cttccctaat tttttctgct
     1981 ccagcccaaa ctgcacgtac agaagcaaac atcaaaggat gaacatagcg attggtgccc
     2041 cacatacgaa ggaaagtacc tgtacctacc ataatagtga ccttaaattc agcacagatt
     2101 cgacctatca tctttgcatc tgttggatca ggatatgcaa ccacaggaac gccttccaca
     2161 atacacatca tagaagtaat agaaaagcca aaagcatgaa acagaggtaa tacaccaagc
     2221 atcacatcat ctgatccagg attaagaacg ctcatacatt gtttaatgtt tcccattaaa
     2281 ttgaaatgag ttaactcgac ccctttagga tttccttctg atccagaact aaataagatc
     2341 gaagcgatat cgtccataga aactttttta aagtcccaaa attcaatcca ccaagcgggt
     2401 aagagtctcg cacgaataaa gtttctaact aaagaaacct ttgataattc ggcttttaga
     2461 tcttccatat atagaatgtt ataactttca aaaagtggtg ttaagtcaag gcctctttca
     2521 gtcaattttg ccacaaaagc ttttgccgta ataatcgttt ttactccgcc ttgatcacag
     2581 caatacttca ttgtcatgac aggacttgta tagtttagat taattaccgt tttcgcttta
     2641 ataaggcaag ctagattagc aataatccca ggggctgttg gaggaaccaa gatggcaatt
     2701 cttttttcac ctggtgtttt tttatcgatc atttttccaa aagttaaacc agccgccgca
     2761 agcgatgttc cagaaaaatg attcccatca gggctaaaaa tagatggcgc actcccgaca
     2821 cgaatagctg ttcgaatcca actagaagcc attggacgaa gagtacgaat ataagtttcc
     2881 caagccgtga tcgataattc ttgtacttta tttttaacaa cgataggaga agaatccatt
     2941 ggcaaa
//
LOCUS       DAOOBB010000218         2939 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_37404_length_2939_cov_4.172677, whole genome shotgun sequence.
ACCESSION   DAOOBB010000218 DAOOBB010000000
VERSION     DAOOBB010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37404_length_2939_cov_4.172677"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1410)
                     /locus_tag="PLT31_08485"
     CDS             complement(<1..1410)
                     /locus_tag="PLT31_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95205.1"
                     /translation="MKKQLKFILMSLLLISFGAQAQTIDPPYSAGTGIWIQQVGTLYP
                     SNPTSATLYYTLYSKENRVKSVDYYYDGNGYLQLSNKVTLCKTTDGLVGADGIVHHPD
                     NDLLIAAQGSKIHKVNKSVGPGNNCLVKTVTGNNYTGNYHLMMDPTNEVVWGAGIPGT
                     LSSYKTTVDPANSSLRDGKQVQLKQKVRNDRFDPNRLSTIIWDEDGTAFYTYSDYFGG
                     GCEASVGGVQGVGPCSAEQQKKSRSKAHFGMFSDTIPQGAKGSAIDIIQTSILIDSLE
                     GAHGGTYDTYSKTIFVFGGSKIVQIDPRTRKVVASIDLRQYFFIESLDKLTMPRTKES
                     DYSYVGWRLDQGTVDGLGHLFVASNTGHLIFIDYTSNPNKYINDNIHIHMQWLDNYLD
                     DVAPLSGVGSSRHNASTGTDEEYSSSSRNYSSSSHALIESSSSVITSSSSGGGTGSSS
                     SGGGTGTSSSDGGTGTSSSD"
     gene            complement(1430..>2939)
                     /locus_tag="PLT31_08490"
     CDS             complement(1430..>2939)
                     /locus_tag="PLT31_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95206.1"
                     /translation="ASLIKGSEEVDDTLVVRFNKDIDPEFEEGDAFYVNGDLVFVQAI
                     KKEGDLWRFAVEKGSVAKGDSISISISGGIVAADGNKTKNNQKVIVTDAGRTYISNEK
                     NGFYDRNDDGQMDSMSVGFESPITREQLDSMDFRFYWLDSTGSVVEIKPDPKDLSLSS
                     DGLVVGYAIDPEDYGIMKNLTSIDSSYSAKGEEYGYAARISKITVDGKDHTEYDYFDM
                     NDRMAPVIKTTFLNPESKRTVFPDELVIEFSEAIDYEKIKSIDGFISFGIDGDWGVTD
                     LTNAHWSEDGKTLSLYISQGSKLFDRANPGDSLRLEKVKDGILDLAGNTVSETSPVVM
                     IQGDPRVVMETASLVSLERAVLLADKAAFTERFFPPGTSVKEEMGKSLGVMLDVAFST
                     ILGDSAKEELDLHSAGLYWELYVYTNLGAYVASAKGEIKCDDDSFGGNCFENQNRLYL
                     RWNMRSEEGRKVGVGVYVAKFMIKVYGARESYETYRVFNWGIKSGKDGLSLE"
BASE COUNT          935 a          664 c          443 g          897 t
ORIGIN      
        1 atcagaagag ctagttcctg ttccgccatc agaagagcta gttcctgttc cgccaccaga
       61 agaactactt cctgttcctc caccagagga actactagta atcacactac ttgaagattc
      121 gattagagca tggctacttg aactatagtt acggctacta cttgaatact cttcatctgt
      181 accagtagaa gcattatgcc ttgaagaacc cactccagaa agaggagcca catcatccaa
      241 atagttatct aaccattgca tgtgaatatg aatattatca ttaatgtact tgtttggatt
      301 tgaggtataa tcaataaaaa tcaaatgccc tgtattacta gccacaaata aatgacctaa
      361 accatctacc gttccctggt ccagacgcca acctacataa gagtaatcac tttctttagt
      421 tctaggcata gttaatttat caagggattc aataaaaaag tactgacgta aatcaataga
      481 ggccaccact ttccttgttc ttgggtctat ttgaacaatt ttagaaccac caaatacaaa
      541 gatcgtcttt gaataggtat cataagtacc accatgagca ccttctaatg aatcaattaa
      601 aatacttgtt tgaatgatat cgatagcact tccctttgcc ccttgaggaa tcgtatcaga
      661 gaacatgcca aaatgagcct tagatctaga ttttttttgt tgttcagcac tacatggacc
      721 aactccctga accccaccga cactggcttc acaaccgccg ccaaaataat cagaataagt
      781 ataaaaagca gtaccatctt catcccagat aatcgtagat aaacgattag gatcaaaacg
      841 atcattacgt actttctgtt ttaattgtac ttgtttacca tcacgtaagc ttgaatttgc
      901 tgggtcaact gtcgttttat aagaagaaag tgttccagga attccagctc cccatactac
      961 ttcatttgtt ggatccatca tcaaatgata gtttccagta taattatttc ctgttaccgt
     1021 ttttacaaga cagttattac ctggacctac ggatttattg actttatgaa tttttgatcc
     1081 ctgtgcggca attaataaat cattatctgg atgatgcaca ataccatcag caccaacaag
     1141 gccatctgtc gttttacaca aagtgacctt atttgatagc tgcaaataac cattgccgtc
     1201 atagtaataa tccacacttt ttacacgatt ttctttacta taaagcgtat aataaagagt
     1261 tgcagacgtt ggattagatg ggtacaacgt accaacctgc tgaatccaaa taccagttcc
     1321 tgctgaataa ggaggatcta ttgtttgagc ttgagctcca aaggaaatga gcagaaggct
     1381 catcaatata aattttaatt gctttttcat tttcttatcc tccaacacct tattctaaac
     1441 ttaaaccatc ttttccagat ttaatacccc aattaaagac acggtacgtt tcatatgact
     1501 ctctagcacc atatacttta atcataaact tagcaacata tacaccaaca ccaactttac
     1561 gtccttcttc ggaacgcata ttccaacgta aataaagtcg attttggttt tcaaaacagt
     1621 ttccaccaaa agagtcatca tcacatttaa tttcaccttt agcacttgca acataagcac
     1681 caagattagt gtacacatat aattcccaat ataaaccagc actatgaaga tctaattcct
     1741 ctttcgccga atcgcctaaa atagtagaga aggcaacatc taacataaca cctagagact
     1801 tgcccatttc ttctttcaca gaagttccag gagggaaaaa gcgttctgta aaagcagctt
     1861 tatcggcaag taatacagca cgttctaagc tgactaaaga ggccgtttcc ataaccactc
     1921 gaggatcgcc ttgaatcata accacaggag atgtttcaga tacagtatta cctgcaagat
     1981 ccaaaatacc atcttttact ttttcaagac ggagtgaatc cccaggattt gctcgatcaa
     2041 ataattttga tccctgagaa atatataaag agagcgtttt tccatcttcg ctccaatgag
     2101 catttgttaa atctgtgaca ccccaatcac catcgatgcc aaaagaaata aaaccatcaa
     2161 tcgattttat tttttcatag tcaattgctt cagaaaattc aatcactaac tcatctggga
     2221 aaaccgttct ttttgattct ggatttaaga acgttgtttt gataactgga gccattcgat
     2281 cattcatatc aaagtaatca tattctgtat gatcttttcc atctaccgtg atctttgaaa
     2341 ttctagccgc ataaccatat tcttctccct tcgcagaata ggatgaatca atagaagtca
     2401 agtttttcat gattccataa tcctcaggat caatcgcata gcctaccact agaccatctg
     2461 aagaaagaga aaggtccttt ggatctggct ttatctcgac cacacttcct gtggagtcta
     2521 accagtaaaa gcggaaatcc attgaatcta attgttctct tgttataggg ctttcaaaac
     2581 caacagacat gctgtccatt tgcccatcat catttcgatc atagaaacca tttttttcat
     2641 tactgatgta tgtacggcca gcatctgtca caatgacttt ttgattattc tttgttttat
     2701 taccatctgc agccacgatt ccaccagaaa tagaaatgga aatggaatct cctttagcaa
     2761 cacttccttt ctctacagca aaacgccata aatcgccttc tttcttaatc gcttgaacaa
     2821 aaactaggtc accattcaca tagaaagcat cgccttcttc gaattctgga tcaatgtctt
     2881 tgttaaaacg aaccactaac gtatcgtcta cctcttctga acctttgatt aaagaagcc
//
LOCUS       DAOOBB010000219         2936 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_37458_length_2936_cov_4.723013, whole genome shotgun sequence.
ACCESSION   DAOOBB010000219 DAOOBB010000000
VERSION     DAOOBB010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37458_length_2936_cov_4.723013"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="PLT31_08495"
     CDS             complement(<1..728)
                     /locus_tag="PLT31_08495"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPW95207.1"
                     /translation="MKFSRYLVWNFLKTLLLLLFSLIIIFVVIDFMGNIRQWLTREMK
                     EAVDYYWNYLPYIVYLVSPVALLISVIVSIGGMARHLEMTAIQSSGRSPLLTLMPVFW
                     VALFLAAASWWLSETILPDANHRRFEIMQTSQQQKKNARVKYKADFVFIDSERVSWFL
                     KHYSGVTKNGRDVLILIRDNGELEERFDAKSIRWEEQKNEEGYWVLENGYHRRFLSDG
                     TISVRSFQTEKLGNRVRTRPEDLI"
     gene            complement(732..2132)
                     /locus_tag="PLT31_08500"
     CDS             complement(732..2132)
                     /locus_tag="PLT31_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPW95208.1"
                     /translation="MILVRYVLKEHIAPFMAALFVITFLFVVDFLVNILDSVLSKGLP
                     MGTVLEIFVLNLAWMLSLSIPMAVLVACLMAFGRLSGDHEITAVKAAGVAPLSLMRPV
                     MLVASLLSLLLILFNNWVLPEANHRSVELMSAVSRKKPHAFIDAGSLITQFPDVQIWI
                     NHIDPASGTLYGVQVFEMQNKGNPRIVYADSASLEYIDNGATLMFRLRSGENHILDSE
                     NPGKYFRIRFSSQDFAMKNVDDRLDRRDRNYRSDREMPIEMMFDVVEAARENKEKYEA
                     EYVKTLFSSLRELNTTLLGDSIVPKGASIEVKEDSIKYVRALQRLKVQERSQLRIVER
                     AYKRIEGEQKREAQFLVEIHKKFSTSVACIIFVLIGAPLGIMARKGGIGTGIIYSLAF
                     FVIYWASLMGGENFADRLMVSPILAMWFSNIIIGIFGIIITIHMTLDRYSGNSGLFYR
                     IRHFIQWITRLNKKIG"
     gene            2199..>2936
                     /gene="bamD"
                     /locus_tag="PLT31_08505"
     CDS             2199..>2936
                     /gene="bamD"
                     /locus_tag="PLT31_08505"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HPW95209.1"
                     /translation="MQLYIKNTFITALSLASLLIMGCASTSNQQNADADRLAMCKERF
                     SKVEEGFKKEEYGRIKTKLEEILTICPGTGYMEQAQFMLAESHFNLEEWIEARGEYGS
                     FVLNFPGSPFIETAEFRKAVASFNMEYHIARDETQTSVAMKDFERFVSNYPDSPLKDS
                     VSYYYNLLVNRLAEKDFQTASLYYKLEKPKAVVIYLKDFLATYPKSERRFDAYLLMVK
                     SYSDLDQFDMARLFLKEVLNDTSIDAKI"
BASE COUNT          978 a          623 c          488 g          847 t
ORIGIN      
        1 ttaattaaat cttcaggtcg agttcttaca cgattgccca atttttcagt ttgaaaagaa
       61 cgtacggaaa tcgttccatc gcttaaaaag cgacgatgat agccattttc taaaacccaa
      121 tacccttctt catttttctg ctcttcccaa cgaatacttt tggcatcaaa gcgttcttcg
      181 agttctccat tatcccttat aagaattaaa acgtctcgac cgtttttcgt tactccagaa
      241 taatgtttca aaaaccaact gactcgttcg ctatcaataa aaacaaaatc ggctttatat
      301 ttcacacgag catttttctt ttgctgctgc gacgtttgca tgatttcaaa ccgccggtgg
      361 ttggcatctg gtaaaatcgt ttcactcaac caccaggaag ccgcggctaa aaaaagcgcc
      421 acccaaaata caggcatcaa cgtcaataga gggcttctcc cagaggattg tatggcggtc
      481 atttcaaggt gtcgagccat gcctccaata gagacgatga ctgaaattaa aagagccaca
      541 ggagaaacta aataaacgat ataaggcaag tagttccagt aataatcaac ggcttctttc
      601 atttcacgag ttagccattg acggatatta cccataaaat caatcactac aaaaatgatg
      661 attaatgaaa ataataacaa caataacgtt tttagaaaat tccaaactag atagcgcgaa
      721 aacttcataa cttacccaat cttcttatta agacgagtga tccattggat aaaatgacga
      781 attcgataaa ataaaccaga attaccagaa taacggtcaa gcgtcatgtg gattgtgatt
      841 attataccaa aaatgccaat gattatattt gaaaaccaca tcgcgagtat tggagaaacc
      901 attaagcgat ccgcaaaatt ttctccgccc attagactag cccaataaat aacaaagaag
      961 gctagactat agatgatccc cgtaccaata cccccctttc ttgccataat ccctaaggga
     1021 gcgccaatca atacaaaaat aatacacgct acagaagtgc taaatttttt atgaatttcc
     1081 actaaaaatt gagcttctct tttttgttcc ccttctattc ttttataagc tcgttctacg
     1141 atacgcagtt gactacgttc ttgtactttc aagcgttgta gggcacgaac gtattttata
     1201 gaatcctctt tcacttcaat acttgctcct tttgggacaa tggaatctcc taaaagggta
     1261 gtgtttaatt ctctaagcga agagaataat gttttcacat attctgcttc atacttttct
     1321 ttattttcgc gggccgcttc caccacatca aacatcattt ctattggcat ttcacgatca
     1381 cttcgataat ttcgatctcg acgatccaag cgatcatcca catttttcat tgcaaaatct
     1441 tgagaagaaa aacgaattct aaaatacttt cctgggttct ctgaatctaa aatatggttt
     1501 tcaccgctac gtaagcgaaa catcaaagta gcgccattat caatgtattc gagcgaagcg
     1561 ctatctgcat aaacaatacg cgggtttcct ttgttttgca tttcaaaaac ttgaacacca
     1621 taaagagtac cagaagctgg atcaatgtga ttaatccata tctgaacatc tggaaactga
     1681 gtaatcaaag agcccgcatc aataaaagca tgtggttttt ttcttgaaac agcactcatc
     1741 aattctaccg atcgatggtt cgcttctggt aatacccagt tattgaataa aattaaaagc
     1801 aaagacaata agctcgccac taacatgaca ggacgcatta aagaaagtgg agcgactcca
     1861 gcggccttca ctgctgtaat ttcatgatct ccagacaatc gcccaaaagc cattaaacaa
     1921 gcgacaagca cagccatagg aatagagaga gacaacatcc aggctaagtt caaaacaaaa
     1981 atttcaagaa ccgtgcccat cggtagacct ttcgataaga cactatctag aatattaacg
     2041 agaaaatcca ccacaaacag aaaggttatg acaaaaagag ctgccataaa aggagctata
     2101 tgttctttaa gtacatacct gaccaaaatc atttgttctt tttactctgt ttttctaatt
     2161 tagtacaacg aaattacgaa ttcggaaaaa taaattcaat gcaattatac ataaagaata
     2221 catttattac agcactttct cttgcctctt tacttattat gggctgtgca agcacctcaa
     2281 accagcagaa tgccgatgcc gaccgcctcg ctatgtgcaa agaacgattt tctaaagtcg
     2341 aagaagggtt caaaaaagag gaatatggca gaattaagac caaattagaa gaaattttaa
     2401 ccatatgccc agggactggc tatatggaac aggctcagtt tatgcttgcc gaaagtcatt
     2461 tcaatttaga ggaatggatt gaagcccgtg gagaatatgg cagctttgtt ttaaatttcc
     2521 ctggatcccc ttttattgaa acagccgagt ttagaaaagc tgttgcttct tttaatatgg
     2581 aataccatat tgctagagat gaaactcaaa catctgtcgc gatgaaagac tttgaacgtt
     2641 ttgtttcaaa ctacccagac tctcctttaa aagattctgt ttcctattat tacaacctat
     2701 tagtcaatcg tcttgcagaa aaagattttc aaacagcaag cctctattac aagctcgaaa
     2761 aaccaaaagc ggttgtgatt taccttaaag attttctagc gacttatcca aaaagtgaaa
     2821 gacgctttga tgcctatctt ttaatggtaa agagctattc cgatttagat caatttgaca
     2881 tggctcgcct ctttttaaaa gaagtcttga acgatacctc tatcgatgcc aagatt
//
LOCUS       DAOOBB010000220         2897 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_38097_length_2897_cov_4.486277, whole genome shotgun sequence.
ACCESSION   DAOOBB010000220 DAOOBB010000000
VERSION     DAOOBB010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38097_length_2897_cov_4.486277"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..1089
                     /locus_tag="PLT31_08510"
     CDS             55..1089
                     /locus_tag="PLT31_08510"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase/BAAT C-terminal
                     domain-containing protein"
                     /protein_id="HPW95210.1"
                     /translation="MFKKKRPQEPKRPFSYIEKEVSFTNNVDGTVLKGTLTLPDTQKK
                     YPAVILVTGSGAQNRDEEILGHKPFLVIADHLTKAGIAVLRYDDRHFKMPIKKGWSYT
                     TKDFAYDAKAAVDFLEQQENIDSSFIGIAGHSEGGVVAPMVASMDSRVAFIITLAGTG
                     VNGREIAVKQNQYFSENKKDAAFAKIAHEIILNEPDFLLRKKKIWQAVNDFYGRFNLL
                     MKIQFLFAMNITASEWNRFFIMYDPAEALEKVTCPVLALNGEYDIQVLPQDNLAAIEE
                     ALKKAKNTDYTTMVIPKANHLFQIVESGGPKPYSKLVKEYATTEQTITPEVLEIMTEW
                     IIKRYLIAKK"
     gene            1300..1653
                     /locus_tag="PLT31_08515"
     CDS             1300..1653
                     /locus_tag="PLT31_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95211.1"
                     /translation="MPKLSRPDVDEILNLSTSIVIDQKRMGKNLRSTVGTATEINTYL
                     RLLFSRMGQPFIGPSFYFSFNHPEGMCPHCHGLGKQIKINLELFLDKEKTVREGAITH
                     PHYKVGGYLWSEPET"
     gene            1671..>2897
                     /locus_tag="PLT31_08520"
     CDS             1671..>2897
                     /locus_tag="PLT31_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPW95212.1"
                     /translation="MADICRLELVDVLSFLLKINDEVSKPILRKAQFILQQLIEIGVG
                     YLSLERSVGTLSGGESQRVKMSKQMDCDLVDMLYVLDEPSIGLHPRDTENLMKILSRL
                     KEKGNSVFVVEHDPDIIRAAEWIVDIGPKAGQYGGRVIYNGEPEGLKNTESITGKYLQ
                     IQEKPIYKRKQATSFFEIKNATANNLKNISVKIPKGILTCVTGVAGSGKSSLIHECFA
                     KEHPEAILIDQSPIGKSSRANAATFIGVFDLIRKEFASATHSEASLFSFNSKGACLKC
                     NGQGVLTFELHFLDSVKTICDECEGKRYHSDVLEYQFHGKNIADVLDMSVSQALEFFE
                     SSKIKNLLSLLEEVGLGYLKLGQSLSTLSGGESQRLKIATELKKKGNIYIMDEPTTGL
                     HMSDVNHFYQIIKTLVK"
BASE COUNT          995 a          460 c          583 g          859 t
ORIGIN      
        1 tatattaagt tttgctagat tgaatgattt tcaggaaaaa aggaaaacaa cgagatgttt
       61 aagaaaaaaa gaccacaaga accaaaaaga cctttttctt atattgaaaa agaagtttct
      121 tttacaaata acgttgatgg caccgtatta aaagggactt tgactttacc cgatactcaa
      181 aaaaaatacc ctgcggtgat tcttgttact ggctctggag ctcaaaacag agatgaagaa
      241 attcttggtc acaaaccttt tttagttatt gcagatcatt tgacaaaagc aggaattgct
      301 gttctacgct atgatgatcg tcattttaag atgccaataa aaaaggggtg gagttatacc
      361 acgaaggatt ttgcatacga tgcaaaagca gcggtcgatt ttcttgaaca gcaagaaaat
      421 atagattctt cttttattgg cattgcaggc catagtgaag ggggagttgt cgcacccatg
      481 gtagcttcta tggattccag agtcgctttt ataattactc ttgcaggaac gggcgtgaac
      541 ggcagagaaa ttgcagtgaa gcaaaatcaa tatttttcgg aaaataaaaa agatgctgca
      601 tttgcaaaaa tagcgcatga aattattctc aatgaacccg attttttatt aagaaagaaa
      661 aagatttggc aagctgtgaa tgatttttat ggaaggttta atcttctgat gaaaatacag
      721 tttttgtttg caatgaatat cactgcttct gaatggaacc gtttttttat tatgtatgac
      781 cctgcagaag ctctagaaaa ggtaacgtgc cctgtgctag ccttaaatgg agaatacgat
      841 attcaagttt tacctcaaga taatttagcg gcaatagaag aagccttaaa aaaagctaag
      901 aatacggatt atacaacgat ggttattcct aaagcgaatc atctatttca aattgtagaa
      961 tctggtgggc caaaaccata ttcgaaactt gttaaagaat atgcaacaac agaacaaacg
     1021 attactcctg aagtcttaga aataatgacg gaatggatta taaaaagata tcttatcgcc
     1081 aagaaataaa aaaggtttag agatggatcg aagccaaatt attattagga atgctcacac
     1141 caacaattta aaaacattga tattgaaatt ccaaaacata aattagtcgt ttttacaggg
     1201 gtttctgggt caggaaaatc gtcatcactt tttgatacca tttatactga ggctcaaagg
     1261 caattagtag agactttttc cacatttgca cgcactcgaa tgccaaaatt atctaggcca
     1321 gatgtagatg aaattctcaa tttatctacg tcaattgtga tagatcaaaa gcgaatggga
     1381 aagaatttac ggagcacagt aggaacagcg acagaaatca atacttattt acgcttgttg
     1441 ttctctcgaa tgggacagcc ctttataggc ccttcttttt atttttcttt taaccatcca
     1501 gagggaatgt gccctcattg tcacggacta ggaaagcaga ttaagattaa cctagaacta
     1561 ttcttagata aagaaaaaac agttcgagaa ggtgctatta ctcaccctca ttataaggtc
     1621 ggtgggtatc tttggagcga gccagaaacg tgaagctgaa tggcttgagc atggctgata
     1681 tttgtcgctt agaattagtc gatgtacttt cttttttatt aaaaataaat gatgaggttt
     1741 cgaaaccgat tttacgaaaa gctcaattta ttcttcaaca attgatagaa attggcgttg
     1801 gttatttgtc tttagaacgt tctgtaggga ctctttctgg aggggaatcc caacgagtaa
     1861 agatgagtaa gcagatggat tgtgatttag tcgatatgct ttatgtatta gacgaaccat
     1921 ctattggatt acaccccaga gatacagaaa atctaatgaa aatattatct cgtctaaaag
     1981 aaaaagggaa ttctgttttt gtggtggagc atgatcctga tatcattcgt gcggcggaat
     2041 ggattgttga tataggccca aaagcagggc agtatggtgg tcgtgttatt tataatgggg
     2101 agccagaagg gttaaaaaat acggaaagta taactgggaa atacctacaa atacaagaaa
     2161 aacccattta taaaagaaaa caagccacgt ctttttttga aataaaaaat gcaacagcca
     2221 ataacttaaa aaatatttcg gttaaaattc caaaagggat attaacgtgc gttacaggcg
     2281 ttgctggcag tggaaagagt agtttaattc atgagtgttt tgctaaagaa catccagaag
     2341 caattcttat agaccaatct cctattggca aatcttctag agcaaatgca gccacattta
     2401 ttggtgtatt tgatttgatt cgaaaagaat ttgcatcagc aacgcactcc gaggcatcgt
     2461 tatttagctt taattcaaaa ggagcttgcc tcaaatgcaa tgggcaaggc gtattaactt
     2521 ttgaactcca ttttttagat tctgtaaaaa ccatttgtga tgaatgcgaa gggaaacgat
     2581 accattcgga tgttttagag taccaattcc atgggaaaaa tattgcagac gttctcgata
     2641 tgagcgtttc tcaagcatta gaattttttg aatcttcaaa aatcaaaaat cttttatcac
     2701 ttttagaaga agtcggttta ggatacttaa aattagggca gtctctaagc acgttatcgg
     2761 gcggagaatc tcagcgctta aaaattgcca ccgaactaaa aaagaagggc aacatttata
     2821 ttatggatga gcctacgaca gggctgcaca tgtctgatgt gaatcatttt tatcagatta
     2881 taaagacttt agtaaaa
//
LOCUS       DAOOBB010000221         2887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_38257_length_2887_cov_4.447740, whole genome shotgun sequence.
ACCESSION   DAOOBB010000221 DAOOBB010000000
VERSION     DAOOBB010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38257_length_2887_cov_4.447740"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(274..777)
                     /locus_tag="PLT31_08525"
     CDS             complement(274..777)
                     /locus_tag="PLT31_08525"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HPW95213.1"
                     /translation="MNTFENIPVESLEINPFERIGKNWMLITAKKGDQVNTMTASWGG
                     LGTMWNKNVAFIVVRPQRYTKEFIDASSTFSLSFFDKKYVKELAYCGKFSGRDENKIE
                     KCNFTVGEEKNTPYIEQANLVMICKKIFVQPYTADSFLDSHIMEFYEKKDFHTLYIAE
                     IESILKK"
     gene            853..2025
                     /locus_tag="PLT31_08530"
     CDS             853..2025
                     /locus_tag="PLT31_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95214.1"
                     /translation="MEKKHFFLLIWVVLLASCLYDPEPYQDSSSTKQESTSSDMGTIF
                     PINTGKQICNPSISNDTVLYPASILWLNFSGALVVKDPPSDFTVNKVVQHDRLTVSDT
                     AGRVQWFVMRDSIGVECQFQDPEWSTHPDYIVALGGFNAPGIKNCSELDYGIMGIRLA
                     DKKAFWFVDSNIIEEAFPHLWVDPNVNADTSALDSLEEFFGTKFVQLVYVDKNDKIAW
                     VDYSKSNKITHLKKPSDKSSWRVDSPLISPDGKWVIYNLMNNAYSWEAYIQELSANSD
                     PIPIPFEQSMMSAPAQPHWWLFQNRLFIVWAEFPSGQTMLNKSDFLDASVYDGSVGRT
                     AMREVSLIAGGIPSDMAVEWKGSVREIAKVPMTGGRSPDGHFMATGTNYGYILELP"
     gene            2032..>2887
                     /locus_tag="PLT31_08535"
     CDS             2032..>2887
                     /locus_tag="PLT31_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95215.1"
                     /translation="MKQKFLLISLMFFAFFMAFLVPVEPTTPWESIGGCGAGGSGGGS
                     GDGIKWIGQGVSGGVLNAEVFVKKSVGQNFSSLSFTPRFSVKPFWATEIGLTLPFMSH
                     QGEVQYRSNQVPVDRTTGGFGDISFDVSQTIGSGGAASLSLALSIPTGQYDIKRGTDA
                     SLEFLPSGFQKGSGLYSLTLGWNYSRDTDKGIWLYSLSYSHPFAMRLWSGENEFNDSY
                     WSELDTKGDDRFDYRFKFYGENDLGAYTPSSVSASIAYGYRGQRGFVHSFGFNFSVPL
                     DVAWIASEK"
BASE COUNT          790 a          514 c          584 g          999 t
ORIGIN      
        1 cattatatat aaagttaatt tttttaactt ctgaaaacgt gatacgtttt tatttgaaga
       61 tcattacgta taaagtaatc ttctaaaatc aaaaaaagcg acacgttttt attgctatat
      121 aattacatat aaagtaatct tctgaaatca aaaaaagcga tacgttttat tggtatatca
      181 ttacatataa agtaatcatc taaaatcaaa aaaacatcgt gttttactag ctcatcatta
      241 cacgcaaagc tatttcccta tgatggggct cgtttatttt tttaagatgc tttcgatttc
      301 tgcaatatat agggtatgga agtctttctt ttcgtaaaac tccataatat gagaatctaa
      361 aaagctatct gcagtatagg gttgcacgaa aattttctta cagatcatta caaggtttgc
      421 ttgttctatg taaggtgtat ttttttcttc acctacagta aaattgcact tttcaatttt
      481 gttttcgtct cttccagaaa attttccaca ataagctagt tctttcacat atttcttatc
      541 aaaaaaggaa agtgaaaagg tggaagaggc gtcgataaat tctttagtgt aacgttgagg
      601 acgaacaaca ataaaggcca catttttatt ccacatggtg ccaagtcctc cccaagaggc
      661 ggtcatggta ttcacttgat cgcctttttt ggcggtgatg agcatccagt ttttaccaat
      721 tctttcaaaa gggttgattt caagagattc gacaggaatg ttttcaaatg tattcatatt
      781 agcatccttt attttgtttt tatgaaagta gaaaagattt tttagtaaat caattttttt
      841 atattgtctt caatggaaaa aaagcatttc tttttattaa tctgggtcgt attattggct
      901 tcttgtcttt atgatccaga accttatcaa gattcttcgt ctacgaaaca agaatctact
      961 tcctctgata tgggaaccat ttttcccatc aatacaggta agcaaatttg taatccttcg
     1021 atctctaatg atacggttct ttacccagct tcgattctct ggttgaactt ttcaggagct
     1081 ctggttgtaa aagatcctcc ttctgacttt accgtcaaca aggttgttca acatgatcga
     1141 cttacggttt cagatacagc tggccgagtc caatggtttg tgatgcgcga ttctataggt
     1201 gtagagtgtc agttccaaga ccctgaatgg agtacgcatc cagattatat tgtggctctt
     1261 ggtggcttta atgcccctgg aattaaaaat tgttctgaat tagattacgg cattatggga
     1321 attcgtttag ccgataaaaa ggctttctgg tttgtggata gtaatattat agaagaggct
     1381 ttccctcatt tgtgggtaga tcctaatgta aacgccgata cttctgctct cgattcttta
     1441 gaagaatttt ttggcaccaa gtttgtacaa ctagtctatg tcgataaaaa tgataaaatt
     1501 gcttgggtcg attattctaa gtcaaataaa ataacccatc ttaaaaagcc aagtgataaa
     1561 tcctcatggc gagtggatag tcctttgatt tctcctgatg ggaaatgggt tatctataac
     1621 ttaatgaata acgcgtattc ttgggaagct tacatacaag aattgtctgc aaattctgat
     1681 cctattccaa ttccttttga acagtctatg atgtctgctc cagctcaacc tcattggtgg
     1741 ctgtttcaaa atcgactttt tattgtttgg gctgaattcc cttctgggca aacgatgttg
     1801 aataagtcag actttttaga tgcttctgtc tatgatggtt ctgtcggtag aacggccatg
     1861 cgtgaagtct ctttgattgc tggtggcata ccttcggata tggctgtaga gtggaaggga
     1921 agtgtacgtg aaatagcaaa agtccctatg actggtggac gctcaccaga tggtcatttt
     1981 atggcgactg gtacaaatta tgggtatata ttggaacttc cctgagggca tatgaaacag
     2041 aaatttttat tgattagttt aatgtttttt gccttcttta tggcgtttct tgttccagta
     2101 gaaccaacaa ccccatggga atctattggt ggttgtggcg ctggcggctc tggaggcggc
     2161 tctggagatg gcatcaaatg gatcgggcaa ggcgtctctg gcggtgtctt aaatgcagaa
     2221 gtttttgtca aaaaaagtgt tggccaaaat ttttcttcac tctcctttac gcctcgtttt
     2281 tcagtcaagc ctttttgggc tacagaaata ggccttacac ttccatttat gtctcaccaa
     2341 ggtgaagttc agtaccgctc gaatcaagtt cctgtggatc gcaccacagg tggttttgga
     2401 gatatttctt ttgatgtttc tcaaactata ggaagtggag gagcggcgtc attatcgtta
     2461 gcactttcta tccctacagg gcagtacgat attaagcgag gtacagatgc ttctcttgaa
     2521 tttttgccat cagggtttca aaaaggctct gggctctatt ctttaacact tggatggaac
     2581 tattctcgag atacagataa aggaatctgg ttatacagtt tatcttactc acatccattt
     2641 gccatgcgtt tgtggagtgg agaaaatgaa ttcaatgatt cttattggtc agagctagat
     2701 acgaaaggtg atgatcgctt tgattatcgt ttcaaatttt atggcgaaaa tgatttaggt
     2761 gcttacactc cttccagcgt gagtgcctcc attgcctatg gctaccgtgg gcaaagaggt
     2821 tttgtgcatt ctttcgggtt taatttctcg gtgcctcttg atgtcgcttg gatcgcttct
     2881 gaaaagg
//
LOCUS       DAOOBB010000222         2860 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_38737_length_2860_cov_3.971480, whole genome shotgun sequence.
ACCESSION   DAOOBB010000222 DAOOBB010000000
VERSION     DAOOBB010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38737_length_2860_cov_3.971480"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            343..1326
                     /locus_tag="PLT31_08540"
     CDS             343..1326
                     /locus_tag="PLT31_08540"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HPW95216.1"
                     /translation="MIRIGIVGYGNLGRGVECAIKNSSDMELVAVFTRRDPKTVSILT
                     KGVPVVHSDLAEEWKDKIDVMILCGGSATDLPAMTPKFVEMFNVIDSFDTHAKIPTHY
                     ANVDEVAKRKHHLALIAVGWDPGMFSINRLFGNAILPQGKDYTFWGKGISQGHSDAVR
                     RIAGVKNAKQYTVPIKEALDAVRAGLDPELSTRQKHTRECFVVLEPGANASAIENEIK
                     TMPNYFADYDTTVHFISEEEFAKNHSGMAHGGFVIRSGKSGMSLENSHIIEYSIKLDS
                     NPEFTTSVLVAYARAAYKMAKEGAYGCKTVLDVPPAYLSEKSGEELRASLL"
     gene            1359..>2860
                     /locus_tag="PLT31_08545"
     CDS             1359..>2860
                     /locus_tag="PLT31_08545"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPW95217.1"
                     /translation="MLDFHPLSLSDKHWINSYLEKLRDPICDYSFANLFIWKHLFNTA
                     CAKEKGFLLIRFCNPFSHHCFYLEPLGEGSLAELIETLKEDIHQKNEILRFMSLSSSF
                     IERLKELDLSNDFFILENRDFANYVYGSKALCTLSEKGLEAKRNHLRRFERLYPNYTS
                     RPLTAEDASLALSLFDQWKRQKEDLSEYSIERQSIETAFAHFSALELEGIILFVDDTA
                     IAFSFGSRLNSNTFCIHAEKANALYQGSFVMINKLMAQTLCERFEFINREEDLGILNL
                     RKAKLSYHPKKMISNFQAFEKESDEMAVWNLWKTCFNDSDEFIFSYLSSYSTPETRVL
                     HYENQKLISMFHVHLFESDWGKVSYLYGLGTEPEYRGKGKAALVIEESLKRSKEANCI
                     AAWVIQENKDFKSWDSTFDFAAVGETVLQFVTPDNFEFGGDDKNDFGLCRLLAPKEYL
                     QKYVDLHPDAKIDFDYHDRIFPEVSGRYQSNHGLITFTPSSSFSKEVLNP"
BASE COUNT          884 a          532 c          524 g          920 t
ORIGIN      
        1 tgccttttgt tctactttct ctaataaaga aaagacctct ttttttttag tagccacaaa
       61 agattctaca ggtttcttaa actgcaacca atttttacat ttagaatcat acactaaaac
      121 tttcataaag catccatgcc cgaagtattg attctttgaa attagtaaaa aacgcccttt
      181 tttgttaata gcaataaaaa tcttcaaatt caaacgatct attcttttca aataaaaaaa
      241 agacctgcct gattggttta tgaaaagcct ttttcacaaa tgatcaaaaa ctgatctttt
      301 tatatttaac aaccaaaatg cacttacaaa ggactttcgt atatgattag aattggtatt
      361 gtcggttatg gtaatttagg aagaggcgtc gaatgcgcca ttaagaactc ttcagatatg
      421 gaactcgtgg ccgtttttac tcgtagagat ccaaaaacgg tttccatttt aacaaagggt
      481 gtaccagttg ttcattccga tctagctgaa gaatggaaag ataaaattga tgtgatgatt
      541 ctttgcggtg gaagcgctac cgacttgcct gctatgacgc caaaatttgt agagatgttt
      601 aatgtaatag atagttttga tacgcacgcc aaaattccaa ctcactacgc caatgtagat
      661 gaagtcgcta aaagaaaaca tcatctcgct ttaattgctg ttggctggga tcctgggatg
      721 ttttctataa accgcctttt tggtaatgcg attcttcctc aagggaaaga ttataccttc
      781 tggggtaagg gtatcagcca aggccattcc gatgccgtac gccgcatcgc tggtgtgaaa
      841 aatgcaaagc aatatacagt tcctataaaa gaagctcttg atgccgttcg tgctggttta
      901 gatccagaac tttctacacg tcaaaagcac actcgtgaat gttttgtcgt tttggaacca
      961 ggagctaatg cctctgccat tgaaaacgaa attaaaacca tgccaaacta ttttgcagat
     1021 tacgatacca ccgttcattt tatttcagaa gaagaatttg caaaaaatca ctctggaatg
     1081 gctcacggcg gttttgtgat tcgctctgga aaatcaggca tgtcactcga aaattctcat
     1141 attattgaat atagtatcaa gctcgattct aatcctgaat ttacgactag tgttctcgta
     1201 gcctacgctc gcgcggctta taaaatggcc aaggaagggg cgtacggttg taaaacggta
     1261 ttagatgtgc ctcctgctta tttaagtgaa aagagcggtg aagagcttcg tgcttcttta
     1321 ctttgatttt ttgaatatct ctaggctttc ataatattat gttagatttt cacccgcttt
     1381 ctctctccga taagcattgg attaattcct acttagaaaa actacgtgat cctatttgcg
     1441 attattcttt tgcgaacctc tttatttgga agcacttatt taatacggct tgtgcgaaag
     1501 aaaaagggtt tttactcatc cgcttttgta atcccttttc tcaccactgc ttttacttgg
     1561 aacctttagg agaaggctct ttagctgaac ttattgaaac attaaaagaa gatattcatc
     1621 aaaaaaatga aattcttcgt tttatgtctt tatcttcgag tttcattgaa aggctgaaag
     1681 agctagacct gtcgaatgat tttttcattt tagaaaatag agacttcgcg aattatgtct
     1741 atggttcaaa agccctttgt accctttcag aaaaaggcct agaagcaaaa aggaatcatc
     1801 ttcgtcgttt tgaaaggtta tacccaaatt atactagtcg acctttaaca gccgaagacg
     1861 cctccttagc actttctctt tttgaccagt ggaaaaggca aaaagaggat ttatctgaat
     1921 atagtataga acgccaatcg atagaaacag cctttgctca cttcagcgct cttgaactgg
     1981 aagggattat tcttttcgta gatgataccg cgattgcttt ttcttttgga tctcgcttga
     2041 attcgaatac tttttgcatt catgcagaaa aagcaaacgc actctatcaa gggtcttttg
     2101 tgatgatcaa taaattaatg gctcaaacct tatgtgaaag atttgaattc attaatcgcg
     2161 aagaagattt gggaatactc aacttaagaa aagcaaaatt atcataccac cctaaaaaaa
     2221 tgatttctaa tttccaagct ttcgaaaaag aaagtgatga aatggctgtc tggaatttat
     2281 ggaaaacttg ttttaacgat agtgacgaat ttattttttc ttatttatct tcatattcta
     2341 ctccagagac gcgagtcctc cactatgaga atcaaaaact tatatccatg tttcacgtcc
     2401 atctttttga aagtgattgg ggaaaagtct cctatctata tgggctcggg acagaaccag
     2461 aatatcgcgg taaagggaaa gcagccctag tgatcgaaga atccttaaaa cgatctaaag
     2521 aagcaaattg catagctgcc tgggtgattc aagaaaataa agatttcaaa agttgggatt
     2581 ctacatttga ttttgcagct gtcggagaaa cagttcttca atttgtcaca ccagataatt
     2641 ttgaatttgg tggagacgat aaaaatgatt ttggtctatg tcgactcctt gctccaaaag
     2701 aatatttaca gaaatatgtt gatttacacc cagatgcaaa aatcgatttt gattatcatg
     2761 accgtatttt ccctgaagtt tctgggcgtt atcagtcaaa tcatgggtta atcacgttta
     2821 cgccgtcctc ctctttttca aaagaagttc taaatcctaa
//
LOCUS       DAOOBB010000223         2826 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_39383_length_2826_cov_3.543486, whole genome shotgun sequence.
ACCESSION   DAOOBB010000223 DAOOBB010000000
VERSION     DAOOBB010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39383_length_2826_cov_3.543486"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PLT31_08550"
     CDS             <1..333
                     /locus_tag="PLT31_08550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HPW95218.1"
                     /translation="NNLQKDLLMNMQKMLKDLQKMQTKMSQAQTNLQSQSFEAEAGGG
                     MVKVVINGQGIITSIKINPNAVDKDDVEALEDLIMAAINAAVKKKDDAASSSMNELTQ
                     GMKIPGLF"
     gene            455..1846
                     /locus_tag="PLT31_08555"
     CDS             455..1846
                     /locus_tag="PLT31_08555"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPW95219.1"
                     /translation="MPKYFAVNIAVLLIMVIPSWSGQIYTREEAVKIALEKASSIQEA
                     EQSLQSATAQVDQAYGNAYPSIDLSVQYARTFGVSDVEQSTSISEMLDASADMNDHII
                     AGTLDGMMYGLSAMKGFRWGTQVGLSANQVLYAQGKVTTGTKIAKVYKRLSEVSLDQA
                     KLQVRFDVEKAFDQVIYLDSAVAIYEASIEQAKKHLDFVNQSFESGLVGELDRVRAEL
                     QVDELSSGLESTKKKQLLAKNALLNTMGLSWTADISFQGDLRDPASFTATLPDTSMTG
                     IRKRRKELVLLDASEEMRELNVKIEEGDYKPMVVLGGSISYAHGGNEWNDWKAPDWDE
                     NISKKVYLSVTMNLFNGMQTKENITMAKTDLRKAQIQKESAERGIRLEVESAVNSYED
                     AQKQITIRTRQVELAKRNLELTEAAYGVGKSPQLDLLDANLSLRNAKLDYMSAVVDWN
                     TAYNALLKATGEY"
     gene            1857..>2826
                     /locus_tag="PLT31_08560"
     CDS             1857..>2826
                     /locus_tag="PLT31_08560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPW95220.1"
                     /translation="MTIQKKLLLLTTVLCSCFLVACEAPKKEEAKKASTIEEIHREKG
                     KPAKVSPVSKQTLADYRIFSGAISGKQQASAIAKMADPIKEIKVSIGSTVKKDQVLAE
                     YLFTGDNTNKEQAEEQVKLLEASVKRLQEVYTKGGFSKQDLEQAETQLRIAKMGLETA
                     RRASVILSPSNGVVTSIDIEQGQVPKLGAAIFTIAQLNEVILTIPVSTKDIGAFKKGL
                     KAYVDINDKTIEGKVTKVPMAANEMTRFFPVEITFNNKGRQLLPGMFVTAKINAKNIE
                     AIAVPNEAIVYQNGNNYVWRVVDGKANRKLVTLGVADASYTQIQSGI"
BASE COUNT          970 a          526 c          555 g          775 t
ORIGIN      
        1 aataacttac aaaaggatct acttatgaac atgcaaaaga tgttaaaaga cttgcaaaaa
       61 atgcaaacaa aaatgtctca agctcaaact aacttacaat cacaatcttt tgaagcagaa
      121 gctggtggtg gcatggtcaa agtagtgatt aacgggcaag ggattatcac ttccatcaaa
      181 ataaacccta atgctgttga taaagatgat gtggaagctc tagaagacct tattatggcc
      241 gctataaacg cagccgttaa gaaaaaggac gacgccgctt cctctagcat gaatgaactg
      301 actcaaggaa tgaaaattcc aggtctattt taatagtcct tttatcgatt attttatcct
      361 gtttatctaa aaaacggcca tttgtactaa acataggcag gtattttttc taatttagca
      421 tctaaattca gtgtttaaat acaaggagat ttaaatgccc aaatactttg cggtaaatat
      481 cgccgttcta ctaataatgg tgataccgtc ttggtctggc caaatttata cccgagaaga
      541 ggcagttaaa atagctcttg aaaaggcttc ctccattcaa gaagctgagc aatccttaca
      601 atcggctact gcacaggtgg atcaggccta tggaaatgca tatccatcta ttgacttaag
      661 tgttcaatat gctcgtactt ttggcgtttc agatgttgaa caatccacta gcattagcga
      721 aatgctcgat gctagtgccg atatgaatga ccacattatt gcagggactc ttgatggcat
      781 gatgtatgga ctaagtgcaa tgaagggttt tcgttggggt actcaagtcg gcttatcagc
      841 aaatcaagta ctttacgccc aaggaaaagt gactactgga acgaaaatag caaaagtata
      901 caaacgctta agcgaagtat ctttagatca agctaagctt caagtccgat tcgatgtgga
      961 aaaagcattt gatcaagtca tttatcttga ttcagctgtt gccatttatg aagctagtat
     1021 tgaacaagca aaaaaacact tagattttgt aaatcaaagt tttgaaagtg gtcttgtcgg
     1081 agaattagac cgagttcgtg ccgaattaca agtggatgag cttagttctg gtttagaaag
     1141 tactaagaaa aaacaacttc ttgctaaaaa tgccctttta aataccatgg gactttcttg
     1201 gacagcagat atctcctttc aaggagatct tcgtgatcca gcctcattta cagccactct
     1261 tccagatacc tctatgactg gaattcgtaa aagacgtaaa gaattagttc ttttagatgc
     1321 ctctgaagaa atgcgcgaat taaatgttaa aattgaagaa ggtgattata agcctatggt
     1381 cgttttaggt ggttccattt cttatgctca tggtggaaac gagtggaatg actggaaagc
     1441 cccagactgg gatgaaaata tttctaaaaa agtctacttg tctgtcacca tgaatttatt
     1501 taatggaatg caaaccaaag aaaacatcac catggccaaa acagacttac gaaaagcaca
     1561 aatccagaaa gagtctgctg aacgcggtat tcgtttagaa gtagaatcag ctgttaattc
     1621 ttatgaagat gctcaaaagc aaattaccat aagaactaga caagttgaac tagccaaaag
     1681 aaaccttgaa ctcacagaag ccgcctatgg tgttggaaaa tcgccccaat tagatttact
     1741 cgatgccaac ttgagtttaa gaaacgccaa attagattac atgtctgcag tcgtcgattg
     1801 gaacactgcc tataacgctt tactaaaagc caccggagaa tattaagagg tttattatga
     1861 caattcaaaa aaaactcctc ctgcttacca ctgttttatg ttcttgcttt ttagttgctt
     1921 gtgaagcacc taaaaaagaa gaagcaaaaa aagcctcaac catcgaagaa attcaccgcg
     1981 aaaaaggtaa acctgcgaaa gtctcccctg taagtaagca aacacttgcc gattatagaa
     2041 tctttagtgg tgccatttct ggaaagcaac aggcatcagc aatcgccaaa atggctgatc
     2101 caattaaaga gattaaagtc agtattggaa gcactgtaaa aaaagaccaa gtgcttgcag
     2161 aatacctttt cactggagat aatacaaata aagaacaagc agaagaacaa gtcaaacttc
     2221 ttgaagcttc tgtcaaacgc ttacaagaag tgtataccaa aggcggtttt tctaaacaag
     2281 atcttgagca agcagaaacg caactacgca ttgccaaaat gggtcttgaa accgctaggc
     2341 gcgcttcagt tatactttct ccaagcaatg gagttgtcac ttctattgat attgaacaag
     2401 gccaagtacc taaattaggt gcagctattt tcaccatcgc tcagctaaat gaagtgattt
     2461 taacgattcc agtttctaca aaagatattg gtgctttcaa aaaaggctta aaagcctatg
     2521 tggatatcaa tgataagacg attgaaggaa aggtgacaaa agttccaatg gctgctaatg
     2581 aaatgacaag attcttccct gtagaaatca catttaataa caaaggacgt caattgttgc
     2641 caggtatgtt tgtcacggct aaaattaatg ccaaaaatat agaagccatt gcagttccaa
     2701 atgaagccat tgtttatcaa aacggtaata attatgtgtg gagagtcgtt gatggaaagg
     2761 caaatcgcaa gctcgtcact cttggtgttg ctgacgcctc ttatacacaa attcaaagtg
     2821 gaattg
//
LOCUS       DAOOBB010000224         2779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_40209_length_2779_cov_3.953010, whole genome shotgun sequence.
ACCESSION   DAOOBB010000224 DAOOBB010000000
VERSION     DAOOBB010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40209_length_2779_cov_3.953010"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..1346
                     /locus_tag="PLT31_08565"
     CDS             168..1346
                     /locus_tag="PLT31_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HPW95221.1"
                     /translation="MIVLDLARSIAGVVGMAASKVPALNEHFSLNDRINGPWPEGPFL
                     WFHGASLGECRMLLNVAKYIQEDIKNCPKILITTQKTDVLEFLQQSGSDISCCIAPAD
                     TPYTMVRFIKKVRPIGLVLAENELWPGYLSSMRKISLKPAVAIISGRFQRAVSKSDFS
                     AIGFACMQSGGDLSRLASLCSPETASKIQIGGDWKLLPWVRSNNGIEAPKNPTVDTTF
                     LSMHFSEWNSLLRMTLSSIKRQESVVIIPRRLEEVDSFRNELRDQELLVVEWPLVQKG
                     AVSLVSRYGQTFEVLSKSKTAVVGGSFARTLGVHDFWEPLQLGVSTCVGPYSHGHKST
                     VDALVREGVLSQLRSTADYAKRDLPDIRLVKTFLTHERAKISESYSQFIAYLEDLIQG
                     "
     gene            1351..2283
                     /gene="ispH"
                     /locus_tag="PLT31_08570"
                     /gene_synonym="lytB"
     CDS             1351..2283
                     /gene="ispH"
                     /locus_tag="PLT31_08570"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HPW95222.1"
                     /translation="MKKILLATPRGFCAGVDRAIHIVEKALEKHGAPIYVRHEIVHNQ
                     FVVSNLKKLGVIFVEDLSEVPENATVIFSAHGVPESVYQEALRRGLKILDATCPLVKK
                     VHSSAKRHYDAGRHVILIGHSGHAEVEGTLGQLPKGALSVVRNKDDIENLSFSKDISL
                     AYITQTTLSVAETKDIIQALKTKFPDIIGPDAGDLCYATGNRQKAVSELSSEVDLLLV
                     VGSKNSSNSSRLCELGLEKGIPSHLIDSVNDIKQEWFKGIETVGVSSGASAPEVLVQE
                     VIRWIKDHFKDIELENRVTLIENTKFNLPKELQD"
     gene            2337..2558
                     /gene="rpmB"
                     /locus_tag="PLT31_08575"
     CDS             2337..2558
                     /gene="rpmB"
                     /locus_tag="PLT31_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HPW95223.1"
                     /translation="MSRICEVTGKAGLVGNMVSHSNRKKLMKQLPNLQKKRFFIPEED
                     RWVTIRVSNAGLRTISKRGIQAVARELGI"
BASE COUNT          834 a          556 c          543 g          846 t
ORIGIN      
        1 tggaaagaca aaaccaatgc gtttcttcct aaaaaaccag ataccaaacc catccaaagc
       61 aataaaagaa gtattcaaaa agcatttttt gccgatcgtt ccacctgggg ctggcttttg
      121 ttttttagtt tgctacttat attgagaaga tatataggag atatgatatg atcgttctcg
      181 acttagcacg tagtatcgct ggagttgtcg ggatggcagc ttctaaagtt cccgcactca
      241 atgaacattt tagcttaaat gatagaatca acggcccatg gccagaaggc ccttttttgt
      301 ggtttcacgg ggctagtctt ggggaatgtc gtatgcttct caatgtcgca aaatatatcc
      361 aagaagatat taagaactgc ccaaaaattt taatcaccac acagaaaacg gatgtacttg
      421 aattcttaca acaatctgga agcgatattt cttgctgtat cgctcctgca gatactcctt
      481 acaccatggt tcgctttatc aaaaaagtcc gtcctatagg gctggttctt gctgaaaatg
      541 agctttggcc aggttactta tcttccatgc gtaaaatctc tttgaagcct gcagtagcga
      601 ttatttcagg ccgttttcaa agggctgtta gcaaatcaga tttctctgcc attggttttg
      661 cttgcatgca atctggtggt gatttatcaa gactcgcttc tctatgttct ccagaaacag
      721 cctctaaaat tcagataggt ggtgactgga aattattacc ttgggttcgt tctaataatg
      781 gaattgaagc gcccaaaaac ccaactgtag ataccacatt tttatccatg catttttcag
      841 agtggaacag tcttcttcga atgactcttt cctcgatcaa gaggcaagaa tccgttgtca
      901 ttattcctcg tcgtcttgaa gaagtcgatt cctttagaaa cgaattaaga gaccaagaac
      961 tcttagttgt agaatggcct ctcgttcaaa aaggtgccgt ctcccttgtt tctagatatg
     1021 ggcaaacttt tgaggttctt tcaaaatcta aaacagctgt tgttggtggc tcttttgctc
     1081 gaactcttgg agtccacgat ttctgggagc ccttacaact aggcgtttcc acttgtgttg
     1141 gcccttattc tcatggtcac aaatcaaccg tagatgctct tgtacgtgaa ggagtgcttt
     1201 ctcaactacg ttccaccgct gattacgcca agagagacct tccagacatt cgtttagtca
     1261 aaacatttct tacccacgaa agagcaaaaa tatctgagtc ttattctcag tttattgcct
     1321 atctcgaaga tttaattcag ggttaatttc atgaaaaaaa ttttactagc aacacctaga
     1381 ggcttttgcg ctggcgtgga tcgagcgatt catattgtcg aaaaagcctt agaaaaacat
     1441 ggtgctccaa tttatgttcg ccatgaaatt gtacacaacc aatttgtcgt gagcaaccta
     1501 aaaaaactgg gtgtgatttt tgtggaagac ttaagtgaag tcccagaaaa tgccaccgtt
     1561 attttttctg ctcatggcgt tcctgaaagc gtttatcaag aagcgcttcg acgcggctta
     1621 aaaatacttg atgccacatg ccctctagta aaaaaagtcc attctagtgc aaagcgtcac
     1681 tatgatgctg gtcgacacgt tattttaatt ggccattctg gacacgccga agtggaagga
     1741 actcttggcc aattacccaa aggggctctt tctgttgttc gaaataaaga cgacattgaa
     1801 aatctctctt tttcaaaaga tatttccctt gcctatatta cacaaaccac tctttccgtt
     1861 gcagaaacaa aagacattat tcaagctcta aaaacaaaat ttccagatat cattgggcca
     1921 gatgcaggtg acctctgtta tgctacagga aacagacaaa aagcagtttc tgagctatcc
     1981 tctgaagtcg atttattatt agtcgttggc tccaaaaact catccaattc aagcagatta
     2041 tgtgaattag gccttgaaaa agggattcct agccatctta tagactctgt aaacgatatt
     2101 aaacaagagt ggtttaaagg aattgaaacg gtaggtgttt ctagcggagc tagtgctcct
     2161 gaagtgttag ttcaagaagt aatccgttgg ataaaagatc attttaagga tattgaacta
     2221 gaaaatcggg ttactttaat agaaaacact aaatttaact tacctaaaga acttcaagat
     2281 tgacgaaatc aaaaaaaata actacaattg agcccgtcta aaaaatggag tcttttatga
     2341 gccgcatttg tgaagttacc ggaaaagccg gccttgtggg aaacatggtt tctcactcta
     2401 atcgtaaaaa gttaatgaaa cagcttccta atctacaaaa gaagcgtttt ttcattcctg
     2461 aagaagatcg ttgggttacc attcgtgtta gcaacgcagg tctccgcact atcagcaagc
     2521 gcggaattca agcagttgct cgcgaactcg gaatctaatt cttccaagcg atagcattta
     2581 tacaatagat tttcaatgat gagaatctat tgttttttct tataaagaaa ctccttaatc
     2641 ggagtttttt tgttttaaat atgagcaaaa cgattatttg ttctccaaaa tgaacgttaa
     2701 tacattgaat ttagcgtatt ttatgcgcaa gacggacaaa gcttctttcg ttggactatt
     2761 taatgagtgg aaggccaag
//
LOCUS       DAOOBB010000225         2731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_41146_length_2731_cov_3.980942, whole genome shotgun sequence.
ACCESSION   DAOOBB010000225 DAOOBB010000000
VERSION     DAOOBB010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41146_length_2731_cov_3.980942"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="PLT31_08580"
     CDS             <1..740
                     /locus_tag="PLT31_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPW95224.1"
                     /translation="LPADLLGTTIYNAKDSCFETRKGPLFTNLVLADEINRAPSKVQS
                     ALLEAMQERSITIGDETFRLEEPFLVLATQNPIEQEGTYPLPEAQVDRFLLKVKVSYP
                     GKTDEMRILESVSGAGLKTPNSVATKDDILNARKLVKSVYVDERIREYIVNLVLATRD
                     PASIKRNDLVSFIEVGASPRASIGLAQAAKAHAFIQGRAYVTPEDVKAVAMEVMRHRL
                     ILSYEAEAEEVSAETVVQKILDSVDVP"
     gene            770..1654
                     /locus_tag="PLT31_08585"
     CDS             770..1654
                     /locus_tag="PLT31_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPW95225.1"
                     /translation="MLSKEVLKTVSRIELSVRGTLDSVMTGAYHSSFKGNGMEFSEVR
                     EYVPGDDIRSIDWNVTARTGTPYVKKFVEERELTMLLMVDASSSSEFGSGLEMKGEVM
                     ATLTALLAFAAIKNNDKVGLLIFTDQVELYISPAKGRKHVLRLIREILYFKPKQHGTN
                     IQNALEYAGKLLNRRAVVVVMSDFLDSGYERAFKVLRKRHDVLAVSVVDPRETELPPV
                     GLVELEDPETGETLLIDTGDPQFREAFVREAKKQTKDMKTMFDRMSVDFVRILIQKEF
                     KNTAAPLLEHFRRRAKKR"
     gene            1672..2625
                     /locus_tag="PLT31_08590"
     CDS             1672..2625
                     /locus_tag="PLT31_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95226.1"
                     /translation="MLLKKWILSTLLFLMLNAGSALAAFDVNVKASVSASQVFIGDIF
                     DYSIEIVAPKEAKVNLPSFIGNLQHFEVKEMKNFREDDKQGQQKVLSKFIWEATLNTF
                     VSGDFFLAPQSVEVIVGKDTVLTKTDPVAIKVMNRTTGEETDILEAEGPITDPRMPTW
                     VWVFLIILGVLLLIVLGWFLHKKWSKKEKAVLLPPYEEAVLAFKELQERHLLEQEEQA
                     EYFTVLGLIIRRYIQRRFDVEILDATLTELRQRMAHVKGLPQAYKESVVLFSEETETV
                     KFAKMKIEKDRCLFWNEWTDRLFEETRPQPEEKKDGVEAKK"
BASE COUNT          864 a          431 c          603 g          833 t
ORIGIN      
        1 tgttgcctgc agacttactc ggaactacaa tctataacgc aaaagattct tgctttgaaa
       61 cacgaaaagg gcctctattt accaatttgg tgcttgcaga tgaaattaac cgtgctcctt
      121 caaaagtaca gagtgctctt ctagaagcga tgcaagaacg ttccattacc attggtgatg
      181 aaacattccg attagaagaa ccatttttag ttcttgccac gcaaaaccct attgagcaag
      241 aaggaacata tcctcttcct gaagctcaag tagatcgttt cttgttaaag gtgaaagtca
      301 gttatcctgg aaaaaccgat gaaatgcgaa ttcttgaatc ggtcagtggt gcagggctta
      361 aaacacctaa ttcagtggca acaaaagatg atattttgaa tgctcgaaaa ttggttaaat
      421 ctgtttatgt agatgaacgt attcgtgaat atatcgtgaa tttagtgctt gctacaagag
      481 atcctgcaag cattaagaga aatgatttgg tgagctttat tgaagtcgga gcttctccac
      541 gtgcttcaat tggtttagct caagctgcaa aggctcatgc gtttattcaa ggacgtgctt
      601 atgtcactcc tgaagatgtt aaagcggttg ctatggaagt gatgcgtcat cgtttgattt
      661 taagttatga agccgaagcg gaagaagtct ctgctgaaac agtggttcaa aagattttag
      721 actcagtaga tgttccataa tcattcatcg ttaactgtat taggtgctta tgctttctaa
      781 agaagtttta aaaacggtga gccgaataga actctctgtt cgcggaactc tagactctgt
      841 gatgacagga gcctaccata gctcttttaa gggcaatggt atggaattta gtgaggtgcg
      901 tgagtatgtt cctggagatg atatccgttc tattgattgg aatgtgacag caagaacagg
      961 aactccttat gtaaaaaagt ttgtagaaga acgtgaactg acgatgcttt taatggtgga
     1021 tgcaagttct agttcagaat ttggaagcgg tctagaaatg aaaggagagg tcatggctac
     1081 attgactgct cttcttgcat ttgcagcaat caaaaataat gataaagtgg gtcttttgat
     1141 tttcaccgat caagtggaac tatatatatc tccagcgaag ggccgtaaac atgttttaag
     1201 gcttattcga gagattcttt atttcaagcc aaagcaacat ggcactaaca ttcaaaatgc
     1261 tctagaatat gcagggaaac tcttaaatcg gcgtgccgtg gtagtggtga tgtctgattt
     1321 tttagactca ggctatgaac gagcatttaa agtgcttcgg aaaagacatg atgtgcttgc
     1381 cgtttctgtt gtagatcctc gtgaaactga attacctcct gtggggcttg tggaattaga
     1441 agatcctgaa acgggagaga ctcttttaat tgatacaggc gatcctcaat ttagagaggc
     1501 ttttgttcga gaggcaaaaa aacagactaa ggatatgaaa acaatgtttg atcgaatgtc
     1561 tgttgatttt gttcgaattt taattcagaa agagtttaaa aatacagcag cccctttatt
     1621 agaacatttt aggcgtcgag cgaaaaaacg ttaatgagaa tggagcaata tatgcttttg
     1681 aaaaaatgga ttttgagcac tttattgttt ttaatgctta atgcaggctc tgctttagct
     1741 gcttttgatg taaatgtgaa agcatctgtg agtgcgtctc aagtttttat tggtgatatt
     1801 tttgactatt caatagaaat tgtggcacca aaagaagcaa aagtaaatct accaagtttt
     1861 attggaaatc ttcaacactt tgaagttaaa gagatgaaga attttcgaga ggatgataag
     1921 caaggtcaac aaaaagtgct atctaaattt atttgggaag caacactaaa tacttttgtg
     1981 agtggagatt ttttcctcgc tcctcaatct gtagaggtga ttgttggaaa agatactgtt
     2041 ctcactaaaa cagatcctgt ggcaataaaa gtgatgaata gaacgacagg agaagaaacc
     2101 gatattttag aagcggaagg cccaataacg gatcctcgta tgccaacttg ggtatgggta
     2161 tttttaatta ttcttggagt tttattgctc attgtcttag gctggttttt acacaagaaa
     2221 tggagtaaaa aagaaaaggc tgttctatta cctccttatg aagaagcggt tcttgctttc
     2281 aaagaattac aagaacgtca tttattagaa caagaagaac aagctgagta ttttactgtt
     2341 ttggggctga ttattagacg ttatatacag agacgttttg atgtagaaat tttagatgcc
     2401 acgcttacag aacttcgtca gcgtatggca catgtgaagg gtttacctca ggcatacaaa
     2461 gaatcggttg ttctattctc tgaagagaca gaaacagtga agtttgcaaa aatgaaaata
     2521 gagaaagacc gctgcctctt ttggaatgaa tggacagatc gactctttga agaaactcgc
     2581 cctcaaccag aagaaaaaaa agatggagtg gaggctaaaa aataatggat ttattgggtt
     2641 atcatattca aaatccggaa gcatttattt tattgctttt aattccggtt ttaattgctg
     2701 attatatcta taacttaaaa aaacgtaaga g
//
LOCUS       DAOOBB010000226         2729 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_41187_length_2729_cov_5.637996, whole genome shotgun sequence.
ACCESSION   DAOOBB010000226 DAOOBB010000000
VERSION     DAOOBB010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41187_length_2729_cov_5.637996"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..549)
                     /locus_tag="PLT31_08595"
     CDS             complement(88..549)
                     /locus_tag="PLT31_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95227.1"
                     /translation="MKPIFSSLFEEDDLFYGTVSFIAIPSVFGNSNIPFLDKQMFNIN
                     NDKNANFHLYLSRQQKIEMQEWFRWWDAEEQMPIALEIKAARINKSTWIVKELNTFDG
                     SISKEVLDDYHLRIAFWGLFIETILLFTSIHFIRFFLKQKPSIEPPIPNNS"
     gene            complement(654..1442)
                     /locus_tag="PLT31_08600"
     CDS             complement(654..1442)
                     /locus_tag="PLT31_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95228.1"
                     /translation="MNTTESRSIQNILKKKIKTPLIWIVIVITIGLTSLFYVSQEQQA
                     VVYNRYVENLSEYKFLETRLMRSLEKTRVGSIEDSTKTATQIMSLREIAISISSSIEN
                     FRLQGEWMPPSSDVILFEREIFGKTGAMRQYLARRNNWSREVKIINDTLYKIPEIETG
                     ILLSCLDSARLGYTPSLPETLRISPEIIANIKDLLAENDDLFILWDRFDNDNALILSE
                     NLLQAFKIKSINDEIFKSRVPQVFYFLSIILLLSTLFFVFRSKT"
     gene            1477..2661
                     /locus_tag="PLT31_08605"
     CDS             1477..2661
                     /locus_tag="PLT31_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95229.1"
                     /translation="MRKIIFLLFLTFFFSGCFERAEEKVFAIHPNGYKKTSFWVYPNG
                     EILKRNEWYSNGIKELEIPYKKNEPHGRFKRWTVFGDVAGVGYYKKGKKEGKWETFFD
                     NKKVESRRFYKNDKKVGTWEGFFYNRNPAWIEYYQNDSAVGLWQKWYSNGVLEEENSC
                     HLAEAEGFKKHYYADGKPQWKWNCRFGIKEGEATQYYPSGLLQLKYRYRNGQLNGPLN
                     IYRANGRLWKREYWKQDVRDSIWAWYDTSEVLIRESRFHNGTGTAFGFCPSGDARLTC
                     AESTFVKNRLDGKLYYYKEKSDLRFEEVWKDGVIKESRSFYPDSLGGGLASEGFWKEG
                     KRNGLWRNWYASGILMDSLHFLNGERYGHQLSYDSTGKLYMHKELFGKNKPVIMHLLN
                     DD"
BASE COUNT          906 a          410 c          556 g          857 t
ORIGIN      
        1 tttaaaacaa aaacttgagt taagaaaaag aaaaaaaaga acactgcttt catttgaaaa
       61 agaactctga acagtttaga aaatctttta ggagttatta ggaatagggg gctctataga
      121 gggcttttgt tttaggaaaa aacgtataaa gtgtattgag gtgaataaca aaatagtttc
      181 aataaataag ccccaaaatg cgatccgtaa gtggtagtcg tctaatactt cttttgatat
      241 cgaaccatca aaagtgttta gttctttcac aatccaggtg cttttattga ttcgggccgc
      301 tttgatttct aaagctatgg gcatttgttc ttctgcatcc caccacctga accactcttg
      361 catttctatt ttttgttgcc gagataaata caaatgaaaa ttagcgtttt tatcattatt
      421 gatgttaaac atctgtttgt ctaaaaaagg aatattggaa tttccaaaca cgcttgggat
      481 cgcaataaag cttaccgttc cataaaacag atcatcttct tcaaataaac tactgaaaat
      541 cggcttcata tatccaaaaa gccaaaccat tataaacgtg gaaaaaatgg ctaaatttaa
      601 agcggaaatt tgcttatagg attttcgtct aggagccatt atctaacctt attttatgtt
      661 tttgaacgga aaacaaagaa caatgtagat aataaaagaa taatagagag gaaatagaaa
      721 acctgaggta ctctagattt gaatatttca tcgttaatgg atttgatttt aaaagcttgt
      781 aataagtttt cactgagtat taaggcgtta tcattgtcaa agcggtccca caaaataaat
      841 aaatcatcgt tttcagccag aaggtctttg atgttagcga taatctcagg cgagattctt
      901 aaggtttcag gaagagaagg agtataacca agccttgctg aatcaagaca gcttaacaat
      961 attcctgttt ctatttctgg tattttatac aaagtatcgt tgattatttt aacttctcta
     1021 gaccaattat tccgtctagc aagatactgg cgcatagccc cagtttttcc aaagatttct
     1081 ctttcaaaaa ggatgacgtc agaagatggt ggcatccatt ctccttgaag tcggaagttc
     1141 tcgatcgaag aagaaataga aatggctatt tctcgcaagc tcataatttg agtggctgtt
     1201 ttagtagaat cttctatcga cccaactctt gttttttcaa gcgagcgcat gaggcgcgtt
     1261 tctaaaaact tgtactctga aagattttca acataacgat tatataccac cgcctgttgt
     1321 tcttgagaga cgtaaaatag tgaagttaac ccgattgtga taacgatcac gatccaaatt
     1381 aaaggcgttt taatcttttt ctttagaatg ttttgaatgc ttcgagattc agtagtattc
     1441 atatttttga aattaccttt tttctacctt atacatatga gaaaaatcat ttttttgctg
     1501 tttttgacgt ttttttttag cggttgcttt gaacgagccg aagaaaaagt ctttgcaata
     1561 caccctaacg gctataaaaa gacttccttt tgggtatatc ctaatggaga aatcttaaaa
     1621 agaaatgaat ggtatagtaa tggcattaaa gaattagaaa tcccctataa aaaaaatgaa
     1681 ccgcatggcc gatttaagcg ctggacggtt tttggggatg tggctggagt tgggtattat
     1741 aaaaagggaa aaaaagaggg gaaatgggaa actttttttg ataataaaaa agtggagtct
     1801 cgccgctttt ataaaaacga caaaaaagtg gggacttggg aaggcttttt ttataatcga
     1861 aatccagcat ggattgaata ctatcaaaat gactccgcag tagggttatg gcaaaaatgg
     1921 tattctaatg gcgtccttga agaagaaaat tcctgccatc tggcagaggc ggaaggattt
     1981 aaaaaacatt attatgcaga tggaaaacct cagtggaagt ggaattgtcg atttggaata
     2041 aaggaagggg aagctacaca gtattaccca agtggtttat tacagttaaa gtatcgttat
     2101 cgcaatggtc agttgaatgg gcctctaaat atttatcggg caaatggtcg cctttggaaa
     2161 agggaatatt ggaagcaaga tgttcgagat tctatctggg cttggtatga tacctctgaa
     2221 gttttaattc gtgaaagccg ttttcataat ggaactggaa ccgcttttgg tttttgtcca
     2281 tctggagatg ctcgactgac atgtgcagaa tccacgtttg ttaaaaatcg tcttgatggc
     2341 aagctttatt attataaaga aaaaagtgat ttgcgctttg aagaagtatg gaaagatggc
     2401 gttatcaagg aaagtcgcag tttttatcca gatagtttag gcggtggact tgctagcgaa
     2461 ggattctgga aagaaggaaa aagaaatggg ttatggcgca attggtatgc ttcaggaatt
     2521 ttgatggata gtcttcattt cttaaatgga gaacgctatg gccatcaatt gagctatgat
     2581 tctactggaa aattgtatat gcataaagaa ctatttggga aaaataagcc tgtgatcatg
     2641 catttgctaa atgatgacta aaaaatttct aaaattgtag gtattatgag tgaaagtatt
     2701 tctaaactaa aacaagattt tgaagccga
//
LOCUS       DAOOBB010000227         2716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_41453_length_2716_cov_3.783540, whole genome shotgun sequence.
ACCESSION   DAOOBB010000227 DAOOBB010000000
VERSION     DAOOBB010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41453_length_2716_cov_3.783540"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="PLT31_08610"
     CDS             complement(<1..793)
                     /locus_tag="PLT31_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95230.1"
                     /translation="MKKINKVSFLSLVTMTAIAFASVDSLYEIDGSAFNERNKTKGPK
                     ALVKVERHKGNENSFKVKPTSANIPVQDTFRISLKKGAEQWIEMEKNTDYFVCVDDSK
                     GGFWKIKNLSYTKESENCIRLNSGNFVKSGKIQYATRMGNGYTFHLLVGMKYIDLRGQ
                     KHLLGYNKEYKFTHYGKIIYYDSLRYEEMNEVLAVDKYKVTECEFVQTLWDSIPSELG
                     REPFYLQYSDNNLFWINKKKSMIKGGFCNAHDSAAVRVYLNHAFVY"
     gene            1238..>2716
                     /locus_tag="PLT31_08615"
     CDS             1238..>2716
                     /locus_tag="PLT31_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95231.1"
                     /translation="MFFFKLLLLASLVIVNVSFAGSPVIKVDFDVSGRTSNEVSEANY
                     IPWVVTGVASKDTTISGVKIKVEKGSVGTAIKTNWYKGIVQAPYYAKLVGDGILVENG
                     NAGGELSLSFSGLSAGTHSLLTYLNNVDAPTAYTYSKIDVYVNGVKTLSNLVPSNRAV
                     TKAEATTAYVTFNVSAGATATIRFVPSSSATFKNVTINGFELNVENSLKQAQNPSPLD
                     LEDHAEAVSNALTLSWSAAAGANKHRVYVGVDSTSVVNATTSSTEYKGEQTGTTYKVD
                     GLYGLNSYYWRIDEVDGSGVVTRGNVWKFRLARLAFEGAEGYGRMARGGRGGKVVYVT
                     NLNDSGAGSLRAAVENDIGPRTIVFKVAGVITLKSRLTLSSSYVTVAGQTAPGKGITI
                     RSAPFGNGGNDNIFRFIRLRLGAGPTFDGMGMAGSKFSIFDHCSISWTIDEAFSSRNA
                     KNMTLQRTLISEALNIAGHQNYPAGTGHGYAATISGDIGSFHH"
BASE COUNT          786 a          519 c          564 g          847 t
ORIGIN      
        1 catatacaaa agcgtgattc aaataaacgc ggacagcggc agaatcatgg gcgttacaaa
       61 agccgccttt tatcatagat ttttttttgt ttatccagaa aagattgttg tcactatact
      121 ggagataaaa aggctcccga cccagttcgg atggtatgga atcccacaaa gtctggacaa
      181 attcgcattc cgttaccttg tatttatcca cggcgagcac ttcgttcatc tcttcgtacc
      241 gcagggaatc atagtatatt atttttccat agtgggtaaa cttgtattct ttattatatc
      301 caagcagatg cttttgccct ctgaggtcta tatacttcat cccgacaaga agatggaacg
      361 tatagccgtt ccccattctt gtcgcgtact ggattttgcc ggatttcaca aaattaccgg
      421 agttcaggcg gatgcaattt tccgattcct ttgtgtacga gagatttttg atcttccaaa
      481 agcctccttt tgaatcatcg acacaaacga aataatcggt gttcttttcc atttcgatcc
      541 actgttcggc gccttttttt aaagaaatac gaaatgtatc ctgcacggga atatttgccg
      601 aggtgggctt cactttgaaa gagttctcgt tccctttgtg tcgttcgact ttgactaaag
      661 ccttgggtcc tttagtttta tttcgttcat taaacgcaga accatctatt tcatacaatg
      721 aatcaacgga ggcaaatgcg atcgctgtca ttgtgaccaa agaaagaaaa gaaactttat
      781 ttattttttt cataagatac ctagcgttta taacatttgc ttttcgagtt gacttaatct
      841 cattgttgtt ctgttgcata tttccttcat cgttccgtaa acgcctattt gttccagttt
      901 ttacaactgg agtttcacgg tatctcaatc tagggccaga tggcaccatt tatgcacaat
      961 agaacgtata aagaaaaata tgtcagaaaa cgtttcaaag catcccctat ggtaatcgaa
     1021 tattaagttt atttatctaa taataaataa aataaattat ttgtcttttt ttatacgtgc
     1081 tttttctaaa aaaccatgta tatatcgtga tttgccccct aggcttaaca aaaatacccc
     1141 ttttcccttc gttgtctgta gaccacagtt aatttacatg cctattctaa ataaaagagt
     1201 tgcaatctat tttaactcta tgaaaaggag agaaaatatg ttttttttca aactattact
     1261 attagcaagt cttgtaattg ttaatgtgag ttttgctggt tctccagtaa tcaaagtaga
     1321 ttttgatgtg agcggaagaa catcaaatga agtatcagag gctaattata tcccttgggt
     1381 ggtgactgga gtggcttcaa aagacaccac tattagtggc gttaaaataa aagtggaaaa
     1441 gggttctgta ggaacagcca taaaaacaaa ttggtataag gggattgttc aagctcctta
     1501 ttatgccaaa ttagtgggcg atggaattct tgtagaaaat gggaatgctg ggggagaact
     1561 ctccctttcg ttttctgggc tttcggctgg cacacattct ttgcttactt atttaaataa
     1621 tgtggatgcc cctactgcct atacttatag taaaatagat gtgtacgtta atggtgtaaa
     1681 aaccctttcg aatttagttc catccaatcg tgcggtaaca aaagcagagg ctacaacagc
     1741 ctatgttaca tttaatgtga gtgcaggagc tacagctaca atacgttttg tcccttcaag
     1801 ttcagccact tttaaaaatg tgaccatcaa tggttttgaa ttaaatgtgg aaaactcatt
     1861 aaagcaagca caaaacccat ctccgcttga tttagaagat catgccgagg cggtatccaa
     1921 tgctcttacc ctttcttgga gtgctgcggc aggtgcaaat aaacatagag tgtatgtagg
     1981 cgttgattca acgagtgtag taaacgcaac aacttctagt actgagtata agggagaaca
     2041 aacaggaacc acttataaag tagatggatt atacggattg aattcttact attggagaat
     2101 tgatgaagtc gatggcagcg gagtggtgac tcgtggaaat gtatggaaat ttagacttgc
     2161 tcgtcttgct tttgaaggtg cagaaggcta tggccgtatg gcgcgtggcg ggcgaggtgg
     2221 aaaagtggtg tatgtaacga atctaaacga ttcaggagca ggaagcttac gtgcagcagt
     2281 tgaaaatgac atagggcctc gtactattgt ttttaaagtc gcaggagtga ttaccttaaa
     2341 atccagacta actcttagca gttcctacgt gactgtcgca gggcaaacgg ctccaggtaa
     2401 ggggatcacc attcgttcgg ctccttttgg aaatggtggt aatgacaaca tcttccgttt
     2461 tattcgtctt cgtttaggcg ctgggccaac gtttgatgga atgggaatgg cgggctcaaa
     2521 attctccatc tttgatcatt gctctatcag ttggaccatt gacgaagcct ttagctccag
     2581 aaatgcaaaa aacatgactc tccaacgtac tttaatttca gaagcattaa atatcgctgg
     2641 ccatcaaaat tacccagcag gaacagggca tggttatgcc gccacaatca gtggtgatat
     2701 tggtagtttc catcac
//
LOCUS       DAOOBB010000228         2689 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_42021_length_2689_cov_3.673880, whole genome shotgun sequence.
ACCESSION   DAOOBB010000228 DAOOBB010000000
VERSION     DAOOBB010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42021_length_2689_cov_3.673880"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2689)
                     /locus_tag="PLT31_08620"
     CDS             complement(<1..>2689)
                     /locus_tag="PLT31_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HPW95232.1"
                     /translation="PAYLDDAAESRTLNPVITQEFADGAGFFNGGWFVTENKGFLKNV
                     KNTSWANAYSYALYAFYKLILKTPEQAALYGINETKRLEYIEDVVANMIYNLGDMSNY
                     GSTSIVLPTGSIGWKNTTVSYDPIWFSMNTDQDWIYNRYQAGNIFEVLAYADVAKDLT
                     NLSLPTMGSPDWKADAMYQLGINQLNYMLGVNPWDVSFILGVGDKNDAHPHHRAANPE
                     GKNFPGAGYKYRPPTGALFGGVKPGATNSWVPSNKSWEDYHLSETCIDATATFISASM
                     LAAQEIDYTRAPKINVEILHVSMDSAIVKLKLDVRSTGALFYGTSESVLNTTATPDNN
                     VAAIEHEIILRGLKNGTTYYFFAGAFNALNENNMTSKYLVDSTQTPFSFTTLNTVESA
                     IIENVTVCNLSADSAEIMWYTPNGEYESKIYWDTIPHSEASEFAWNSGTKNADISGIP
                     TKFHYVKIGGLKEKTTYYYMVESNGEFQSVDDKGDLLKFTTPVAWYDFSVRTYQYEFG
                     GLDFLDLNIYNNESYAFDSLTLRLYVTAKPEEIEKCAFLVDLDICQAYDEGGFNKPCE
                     TDREIRDLLRNAKAVKLEDTYNAATGTYSWYFPVPLGSTTIKASSRLRMDLGFSQGLS
                     NDGYLTCETLRQPSNKRMTKETGDWSWSPHLKEVDGAFYDGMPFEDKDYGDIDDNIPV
                     NPYIVVYRKDEFIWGYSPSYQEMTTKRANYEISLTLEPPFNVSNGSHVELDQNSSTVY
                     FKGTASVTEGGFITRIWANGTEVKDLASAAKYNVLTDMYDLNIPVKMTIGSNKVDVTV
                     FAGPDPTCQSCTENGGCDFENRNFFVQFTKGNASASNLRITDASGNAISSPAEPNSTS
                     FYIHLSDKDKANYTGVVNALVINGRQKDTLRVKLDPETAG"
BASE COUNT          789 a          533 c          503 g          864 t
ORIGIN      
        1 cctgctgttt ctggatctag tttcacgcgg agtgtatctt tttgcctacc attaatgaca
       61 agagcattca ctacaccagt gtaatttgct ttatctttat cacttagatg gatgtagaaa
      121 gaagtgctat ttggttctgc tgggcttgaa atagcgtttc cagaagcgtc tgtaatacgc
      181 aagttacttg cagaagcatt ccctttagta aattgaacga agaagtttct gttttcaaaa
      241 tcacacccgc cattttctgt acaactttgg caagtaggat ctgggccagc aaaaacagta
      301 acatcgactt tattagaacc aatcgtcatt tttacaggga tgttgagatc atacatatca
      361 gtaagcacat tatattttgc agcagaggca agatctttaa cttcagtacc attagcccaa
      421 atacgagtaa taaaaccacc ttctgttaca ctcgctgtcc ctttaaaata gactgtactg
      481 ctattttgat ctagttcaac gtgagaaccg ttggatacgt taaatggagg ttcaagggtg
      541 agactgattt catagttagc gcgtttggtt gtcatttctt ggtaagaagg actataaccc
      601 caaataaatt catcttttct gtaaacgaca atgtacggat tgacaggaat attgtcatct
      661 atatctccat agtctttgtc ttcaaaaggc atgccatcgt aaaaggcgcc gtccacttct
      721 ttaagatgag gggaccatga ccagtctcct gtttctttcg tcattctctt attagacggc
      781 tgacgtagtg tttcacatgt caagtagcca tcattagaaa ggccctgaga gaagcctaaa
      841 tccatacgaa gtcgagagga cgctttgatc gttgtagaac ctaatgggac agggaaatac
      901 catgaatagg ttccagtggc ggcattatag gtgtcttcta attttactgc ttttgcattt
      961 cgtaataaat ctctaatttc tctatcggtt tcacatggtt tattaaatcc accttcatca
     1021 taagcttggc atatatctag gtcgacaagg aatgcgcatt tttcaatttc ttctggttta
     1081 gcggttacat ataaacgtaa agttaagcta tcaaacgcat aggattcgtt gttgtatatg
     1141 ttcaaatcca gaaaatctaa tccaccaaat tcgtattgat aagttcttac agaaaaatca
     1201 taccaagcca caggagtcgt gaatttgaga agatctcctt tatcatctac cgattggaac
     1261 tctccattac tttctaccat gtaataatac gtggtctttt cttttagtcc accaatcttt
     1321 acataatgga atttggtggg gattccagag atatctgcat tttttgttcc agaattccaa
     1381 gcaaattcac ttgcttcaga gtgaggaatg gtatcccaat agattttaga ttcatattct
     1441 ccattaggag tgtaccacat gatttctgca ctatcggcac tcaaattaca tacagtgaca
     1501 ttttcaatta tggcactttc tacagtattc aaagtcgtaa aagaaaacgg cgtttgagta
     1561 ctatcgacta agtattttga agtcatatta ttttcattta aggcattgaa agcacccgca
     1621 aagaagtagt atgtcgttcc attttttaag cctcttaaaa taatttcgtg ttcgatagca
     1681 gcaacattgt tgtctggagt agctgtagta tttaaaacac tttcactagt gccataaaaa
     1741 agagcgcctg tactacgtac atctaacttt aatttgacaa tggctgaatc catactcacg
     1801 tgaagaattt ccacattgat ttttggtgct ctagtgtagt caatttcttg tgcggcaagc
     1861 atactagcac ttatgaatgt cgctgtagca tcaatacaag tttcagaaag atggtagtct
     1921 tcccagcttt tgtttgatgg aacccaagag tttgttgccc ctggctttac tcctccaaat
     1981 aaagctcccg taggagggcg gtatttgtat ccagctcctg gaaagttttt cccttctggg
     2041 ttagcggcac ggtgatgcgg atgagcatca tttttatctc caacacctaa aataaaagaa
     2101 acatcccaag ggtttacccc aagcatgtag tttaactgat taatccctaa ttggtacatt
     2161 gcatctgctt tccaatcagg actccccatt gtaggcaagc tcaaattggt taaatctttg
     2221 gcgacatcgg cataagctag gacttcaaaa atattacctg cttgataacg attataaatc
     2281 caatcttgat ctgtattcat gctaaaccaa atgggatcat aagatacagt tgtatttttc
     2341 cagccaatag aacctgtagg caaaacgata gaagtagagc catagttact catatcgcca
     2401 agattataaa tcatattggc gacgacatct tcaatatact ctagacgttt ggtttcgtta
     2461 ataccgtata gtgcggcttg ttctggtgtt ttaagtatta gcttataaaa ggcataaaga
     2521 gcgtaggaat atgcattagc ccaagaggtg ttttttacgt ttttcaaaaa acctttattt
     2581 tcagtaacaa accagccacc gttaaagaac cctgcaccat ctgcaaattc ttgcgttatg
     2641 actggattta atgtacgact ttctgcagca tcatcaagat aagctggat
//
LOCUS       DAOOBB010000229         2638 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_43066_length_2638_cov_3.749516, whole genome shotgun sequence.
ACCESSION   DAOOBB010000229 DAOOBB010000000
VERSION     DAOOBB010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43066_length_2638_cov_3.749516"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PLT31_08625"
     CDS             complement(<1..205)
                     /locus_tag="PLT31_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95233.1"
                     /translation="MKQINFLKVLMFMAIILWPTTTQAQTYSGGDGSADSPYEIKTTA
                     DLQALADSVKTTTDYSKGKFFKLT"
     gene            complement(415..2196)
                     /gene="aspS"
                     /locus_tag="PLT31_08630"
     CDS             complement(415..2196)
                     /gene="aspS"
                     /locus_tag="PLT31_08630"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPW95234.1"
                     /translation="MKRTHNCGALRLEDVGKTVSLAGWVDRRRDLGGVIFVDLRDKYG
                     KTQVVFDPERNQEVHQIADQLRNEFVISVTGVVQNRPEGMENDKLSTGKIDVKIDKIE
                     ILNTAETSPLAINDPKEESRENDDLRLRYRYLDLRRPWIQKNLILKSRFLKEVHHFFH
                     ENGFEDIETPVLCKSTPEGARDYLVPSRVNPGRFYALPQSPQQYKQLLMIAGMDRYYQ
                     VAKCFRDEDLRADRQPEFTQIDVEMSFVDQNDVMDLFDRFVSEVIGKVWDFTPPSPIR
                     RMTYAEAMLKYGSDKPDLRFDLEIKDVTELAGNTGFGVFNSVMKAGGKIRGLAAKGCV
                     DFTRKQIDDLTAHVARYGAKGLVWMRVKENDEVETQVGKFFTVDQLNALRDAVGASAS
                     DMMFFIAGSEKIAATAMGQLRLEIARIKGLMNPSVREFVWITEFPMFEYSETEGRYMS
                     MHHPFTSPMPEHLEMMLSGNLKDCNAQAYDLVLNGVEIGGGSVRIHKPDVQEKVFRLL
                     GLNEEQVKEKFGFFVDAFKFGAPPHGGLAFGLDRVVATMEGFESIRDFIAFPKNTSAS
                     SPMDQSPSEVDLTQLQDIHISLNLPKK"
BASE COUNT          801 a          531 c          458 g          848 t
ORIGIN      
        1 cagtgagttt aaaaaacttc ccttttgagt aatcagttgt agtttttacc gagtctgcaa
       61 gtgcctgcaa atctgctgtt gttttaattt cataaggaga atcggcacta ccatctccac
      121 ctgaataggt ttgtgcttgt gttgttgtcg gccaaagaat aatggccata aacattagta
      181 cttttaaaaa atttatttgt ttcatttttg atttatctct cctcaaatca cgccactaat
      241 acaattttac ttttctctaa tataaataat gattcactag ttgtaatata aaccgaagaa
      301 gatgctgatt ttttttatac tagattgaat tcactacata aaaaaagagc ccttttgaag
      361 gactcttttt ttacataatc ctattcatcg ctgaatagga ttaattaaag cgtattattt
      421 tttaggcaaa ttcaaagaaa tatgaatatc ttgaagttga gttaaatcga cttcgcttgg
      481 gctttgatcc attgggctag aagcactcgt gtttttaggg aaagcgataa aatcacgaat
      541 cgattcaaac ccttccatag tcgctactac acgatctaaa ccaaaggcaa ggccaccatg
      601 tggaggagct ccaaacttaa aggcatcgac aaagaaacca aatttttctt tcacttgttc
      661 ttcgtttaag ccgagaaggc ggaatacctt ttcttgaacg tctggcttat gaatacgaac
      721 ggatccaccg ccaatttcaa cgccattgag aacaaggtca taggcttgag cattgcaatc
      781 ttttaagttc cctgaaagca tcatttctaa atgctctggc attgggcttg tgaatggatg
      841 atgcatactc atgtaacgcc cttctgtttc actgtattca aacataggga attcagtaat
      901 ccatacaaat tcacgaacgc ttgggttcat taaacccttg attcgtgcaa tttcaagacg
      961 taattgaccc atcgctgtag cggcgatttt ttctgaacca gcaataaaga acatcatgtc
     1021 agaagcacta gcgccaacag catcgcgaag agcattgagt tgatcgactg taaagaactt
     1081 tccaacttga gtttccactt catcgttttc tttaacgcgc atccaaacaa ggcctttagc
     1141 gccataacga gccacgtggg cagtcaaatc atcaatttgt ttacgagtaa aatcaacaca
     1201 acccttagcg gcaagcccac ggatctttcc acccgctttc atcacggaat tgaacacgcc
     1261 aaagccagta ttaccagcta attctgtcac gtctttaatt tctaaatcaa agcgtaaatc
     1321 tggtttatca gaaccatatt taagcattgc ttcggcgtat gtcatacggc gaataggaga
     1381 tgggggagtg aaatcccaaa cttttccaat gacttcagaa acaaagcgat caaataaatc
     1441 catcacatca ttttgatcta caaaagacat ttccacatca atctgagtaa actcaggttg
     1501 gcggtcagct ctaagatctt catcacggaa gcactttgct acttggtagt agcggtccat
     1561 accagcaatc attagtaatt gtttgtattg ctgtgggctc tgaggcaatg catagaacct
     1621 tcctggattg actcgagacg gcactaaata atcgcgagca ccttctggag tacttttaca
     1681 aaggactggc gtttcaatat cttcaaagcc attttcatgg aagaaatgat gaacttcttt
     1741 taagaagcga ctctttagta ttaaattctt ttgaatccat ggacgtctta agtctaggta
     1801 gcgatagcgt aagcgtaaat cgtcattttc tctagattct tctttaggat cgttaatggc
     1861 taggggagat gtttcagcgg tatttaaaat ttcgatttta tcaattttaa cgtcaatttt
     1921 accagtgctg agcttatcat tttccatgcc ttcaggacga ttttgaacaa ctccagtcac
     1981 agaaatcaca aattcgtttc ttaattgatc tgctatctga tgaacctctt gattcctttc
     2041 tggatcaaaa accacttgtg tttttccata tttatcacgc aaatccacaa aaataactcc
     2101 gcctaaatca cgacggcgat ctacccagcc cgcgagagaa actgttttgc caacatcttc
     2161 taggcgtaaa gcaccacaat tatgagtccg tttcatacaa taaaagtcct taaaagagga
     2221 aaattttgaa caaatataga ataaaggaaa ataaaattta gaggttactt tctcaaaaga
     2281 cttgagaatg atatggtttt gatgaatact tcttcaatga atcgtaattc aaaaaaaaaa
     2341 tccctatttt ttttttagaa aattagtttt tctattttcg agatccaaat gtaatcgtac
     2401 gaggtaatgt ccttgaaaat aaaaaacgca gtaccctatt ttatttcggt tttctgtatt
     2461 tcctttgtgc aaatgggctt atttatcttt tttcaaagat ggctttcttt ttcgtattca
     2521 ggcgttcctt ttttatggag aagcgcttta ttacagacgc ttttattact gccttatatt
     2581 tttatgcttg ctccagcaag ttatttttcc aatcgctatc cgaaaaataa agtcatgg
//
LOCUS       DAOOBB010000230         2622 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_43406_length_2622_cov_3.309700, whole genome shotgun sequence.
ACCESSION   DAOOBB010000230 DAOOBB010000000
VERSION     DAOOBB010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43406_length_2622_cov_3.309700"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..1569)
                     /locus_tag="PLT31_08635"
     CDS             complement(142..1569)
                     /locus_tag="PLT31_08635"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPW95235.1"
                     /translation="MFSNIQGFMADDFGKATVQIDRLKRVYFSDPANRLSLKKGDEIV
                     SQGKYNDSLFLVLSGHVLGKCCIESTEKQDPSDRCFEMFRGGVGTFVGVHSFFSEPYL
                     SFFTVEALDDVEVAFLKRDSAPIEKEKYGSLQEQFIPMISNELVLRNFHLLEKSIDNE
                     SALNRLHRSELNASLGQLAAGLAHELNNAIGVISRKTDFIAEALDRIIESKITEAHDL
                     FHLGENYNAMISSETIRERTRLFEKKKNLSHMAAKVLARLVETEEDLKKLDKKVLDDF
                     ENLSRYWELGHDVRDIRLAAKHAADIVKSVRTLGGSNFTRSEGLSIHETLSRAISLLK
                     PNLHDIKLEVDLESAEKMPLIYGDMTELVQIWVNIIKNSCDAMEAAETKDPKVTIRCS
                     HTKTHVFVHITDNGPGISEESAEKIFQMDFTTKKNGLSFGLGLGLAIVQRIVDSYGGE
                     IILASRPQNTTFNIKLPIENHYGND"
     gene            complement(1559..>2622)
                     /locus_tag="PLT31_08640"
     CDS             complement(1559..>2622)
                     /locus_tag="PLT31_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HPW95236.1"
                     /translation="RLDMNLAKVLLKPFGNNPKYILLGLMIITAIFSMFMSNTATTAM
                     MLAILAPVLALFDSSDKGRAAFALAVPVAANIGGMGTPIGTPPNAIALKALADSGLNV
                     SFGKWMAFGVPYVIILILISWFVLIKLFPIEKKTMELKVGDRFLKTPKAIIVYITFGL
                     TVVLWVLGEGVHGMDSNTVAMIPMAVFALAQIITKDDLKGMSWDVLWLVAGGFALGLG
                     LQATGLAKDLIGAISFDSLPVVALMIGSGFLCLLMANFMSHTATASLLIPIIAAVGTS
                     LGTTLAPLGGLTALIVSVAFASSLGMSLPISTPPNALAHATGFVDTKGMAKVGIVIGV
                     VGMLLTYVMMYLLATFHVL"
BASE COUNT          889 a          569 c          425 g          739 t
ORIGIN      
        1 cctcgttcat ccatttcatc catcaaagac aagcagtctt ctgctgattc agcaccttcc
       61 acacgataaa actgttccaa agaacgtaaa tcgcgtatta cggcatcaag aacatcggat
      121 tgatcgtcga cacatattat attaatcgtt tccataatga ttctctattg gtagttttat
      181 attgaaagta gtattttgag gacgagaagc aaggattatt tcgcctccgt aactatccac
      241 aattcgttga actatagcta accctaagcc caaaccgaaa gaaagtccat tttttttcgt
      301 tgtaaaatcc atttggaaaa ttttttctgc actttcttct gaaattcctg gcccattatc
      361 tgtgatatga acaaagacat gcgtttttgt atgcgaacaa cgaatggtga cttttggatc
      421 ttttgtttca gcagcctcca ttgcatcaca actatttttt ataatattca cccaaatttg
      481 cactaattcg gtcatatcac cataaatcaa aggcattttt tcagcacttt ctaaatccac
      541 ttcaagctta atatcatgaa gattaggttt caagagagaa atggctctag ataacgtctc
      601 gtgaatactt aagccttcgc ttcgagtgaa attactgcct cctaaagtgc gtacagactt
      661 cacaatatcg gcggcatgtt ttgctgctaa acggatatct cgaacatcat gccctaattc
      721 ccaatagcga gacaaatttt caaaatcatc taatactttt ttatcaagct ttttaagatc
      781 ttcttcagtt tccactaaac gtgctaaaac ttttgcagcc atatgtgaaa gatttttttt
      841 cttctcaaat aatcgagtcc gctcgcgaat cgtctcagaa gagatcattg cattataatt
      901 ttcacctaaa tgaaataaat catgtgcttc tgttatcttc gattcaataa tacgatctaa
      961 agcttctgca ataaagtcag tctttcgaga aatcactcca atcgcattat ttaattcatg
     1021 agcaaggccc gctgctaatt gcccaagaga cgcatttaat tcagagcgat gtagtcgatt
     1081 cagagccgat tcattatcaa tagacttttc taatagatga aaatttcgaa gcactaattc
     1141 attcgaaatc ataggaataa actgttcttg aagtgagccg tatttttctt tctctattgg
     1201 agcagaatcc cgttttaaaa aagcgacttc aacatcatct aatgcttcaa ctgtaaaaaa
     1261 acttaagtat ggttctgaaa aaaaactgtg taccccaaca aaagtaccta caccacctct
     1321 aaacatttca aagcagcgat cagatggatc ttgcttttcc gtagattcaa tacaacactt
     1381 tcctaaaaca tggcctgaaa gtacaagaaa aagggaatca ttatatttcc cttgagatac
     1441 aatctcgtca cctttcttta atgacagacg attggctgga tctgaaaaat agaccctttt
     1501 gaggcgatcg atttgaacgg tcgcttttcc aaaatcatct gccataaagc cttgaatatt
     1561 agagaacatg gaatgtcgct aacaaataca tcatcacata agttaaaagc attccaacaa
     1621 caccaattac aatacccact tttgccatgc ctttagtatc cacaaatcca gtcgcatgag
     1681 ctaaagcatt tggaggagta ctaataggaa gactcattcc taaagaactt gcaaaagcaa
     1741 cagaaacgat aagagccgtt agacctccaa gaggagcaag agtggtgcct aaagaggttc
     1801 caacagcagc tataataggg attaaaagag atgctgttgc ggtatggctc ataaaattag
     1861 ccatcaaaag acataggaaa ccagaaccta tcattaaagc aactactggc aatgaatcaa
     1921 aagagatggc tccaattaag tcttttgcaa ggcccgttgc ttgtaaacca agccctaatg
     1981 caaaaccacc ggccacaagc cataatacat cccagctcat acccttcaaa tcgtcttttg
     2041 taataatttg agcaagagca aaaacagcca taggaatcat agcgacggta ttggagtcca
     2101 tgccatgcac tccttcacct aaaacccaca aaacaacagt cagaccaaaa gtgatataaa
     2161 caataatcgc cttaggtgtt tttaagaaac gatcaccaac cttcaattcc atagtcttct
     2221 tttcgattgg gaataactta attaaaacaa accaagaaat caaaataaga ataatcacgt
     2281 atggaacacc aaaagccatc catttaccga acgaaacatt taatcctgaa tccgctaacg
     2341 cttttaaggc aatcgcattt ggaggagttc caataggagt acccatacca ccaatattag
     2401 cagccacagg aacggcaaga gcaaaagcgg cacgtccctt atcacttgaa tcaaataaag
     2461 ctaacactgg agctagaatg gcaagcatca tcgccgttgt agctgtgtta ctcataaaca
     2521 tggagaaaat agccgtaata atcatcaagc caagaagtat atattttgga ttatttccaa
     2581 aaggctttaa taagaccttc gcgaggttca tatctaaacg at
//
LOCUS       DAOOBB010000231         2590 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_44105_length_2590_cov_4.197239, whole genome shotgun sequence.
ACCESSION   DAOOBB010000231 DAOOBB010000000
VERSION     DAOOBB010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44105_length_2590_cov_4.197239"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..1494)
                     /gene="sufB"
                     /locus_tag="PLT31_08645"
     CDS             complement(79..1494)
                     /gene="sufB"
                     /locus_tag="PLT31_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HPW95237.1"
                     /translation="MNENYKYGFVTDIENDAFEKGLSEDIIRRASKLKNEPAFLLDFR
                     LKSYERLLKMTPPTWGELHFPPVDLQDIVYYSAPKNKKQHESIEDVDPELLATFEKLG
                     IPLDEQKRLANVAVDAVFDSVSIYTSHKKKLMEYGVIFCSISDAVKEYPELIEEYMGS
                     VVPAGDNYFAALNSAVFTDGSFVYIPPGVKCPMDLSTYFRINNKEAGQFERTLIIADA
                     GASVSYLEGCTAPEFSSKQLHSAVVELVAKDDAKIKYSTVQNWYAGDRKTGEGGVYNF
                     VTKRGKCAGKNSHISWTQVETGSAITWKYPACLLVGDNSTGEFYSVALTNGHMQADTG
                     TKMIHIGKNTKSTIISKGISADQSSNAFRGLVDIRPKASNSRNYTQCDSMLVGTESAA
                     HTFPYIQVANSSAKTEHEATTSRISEDQLYYFESRGIKREDAIQAMVSGFCKDVFQEL
                     PGEFASEARQLLTLKLEHSVG"
     gene            complement(1501..2304)
                     /locus_tag="PLT31_08650"
     CDS             complement(1501..2304)
                     /locus_tag="PLT31_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014544984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HPW95238.1"
                     /translation="MSEESLSISNKLAQTAKMPKYRGAIFQIEADEKGLALIDIKESS
                     LKVYLMVDPDSDIILETRFFTYGGPIFTALADTFCSLIQNKTVEEASSIKISEIESLL
                     RDTPHTKALPEDAVELELLQKLILNIKEVYPEKKNVALAAREAMEAIKYKTQTAEGRA
                     EADAEWNALSHEEKLQKIEDCLHEYVRDTLRMDGGDLEVLALTKDNRLQIRFQGACAG
                     CGSAMGGTLFYIEDQLKNNVYYNIVVEPEDPFASYPPLTDEEMPQTLTP"
BASE COUNT          749 a          536 c          504 g          801 t
ORIGIN      
        1 gaagtcgatt taatcgctca tggcgtacca caccgcgttt tagggcatca tcatcaccat
       61 aagcattaat ttagaacact agccaacgct atgttctaat ttaagcgtca gaagttgcct
      121 tgcttcagag gcaaattcgc cagggagttc ctggaagacg tctttacaaa aaccactgac
      181 catcgcttga atcgcatctt ctcttttaat gccgcgagat tcaaaataat agagttggtc
      241 ttcactaatt cgactagtag ttgcttcgtg ttctgttttt gcagaagaat tggctacttg
      301 aatataaggg aacgtatggg cggcactttc tgtacccaca agcatgctat cgcattgagt
      361 gtagttacgc gagttacttg ctttagggcg aatatcaaca aggcctctaa acgcattgct
      421 gctttgatcg gcactgattc ccttgctgat aatagtactc ttggtatttt taccaatatg
      481 aatcatttta gtaccagtat cggcttgcat atggccattc gttagagcca cagagtaaaa
      541 ttcaccagtg gagttatcgc caacaagcaa acaagcaggg tatttccatg taatagcact
      601 tccagtttct acttgagtcc agctaatatg gctgttttta ccagcgcact tgcctctttt
      661 tgtgacaaaa ttataaacgc caccttcccc tgtttttcgg tcgcctgcat accagttttg
      721 tacagtgctg tattttattt tagcatcgtc ttttgctacc aattcaacga ctgcactatg
      781 caactgtttg ctgctaaact caggagcggt acacccttcg aggtaactca cgctcgcgcc
      841 tgcatcggcg ataatcaaag tcctttcaaa ttgcccagct tctttattgt tgattctaaa
      901 ataagtggaa agatccattg ggcattttac tccaggcggg atatacacaa aactcccatc
      961 agtgaaaacg gcgctgttta atgcggcaaa ataattatca ccagcaggaa caacgctacc
     1021 catatactct tcaatcaatt cagggtattc ttttactgca tctgaaatag aacagaaaat
     1081 gacaccatat tccatcaact tctttttatg actcgtataa atactcacgc tatcaaacac
     1141 agcatctaca gccacattcg ctaagcgctt ttgttcatca agaggaatgc ctagcttttc
     1201 aaaagtggct agcaattctg ggtcgacatc ttctatcgat tcatgttgct tcttgttttt
     1261 aggagccgag tagtaaacaa tatcttgcaa atcgacagga ggaaaatgga gttcgcccca
     1321 agtaggggga gtcattttca aaagcctctc gtaagatttt aagcgaaaat ccaataaaaa
     1381 agcaggttca tttttaagct tagaggctcg tcgaataatg tcttctgaaa ggcctttttc
     1441 aaaagcatca ttttcaatgt ctgtcacaaa cccgtattta taattttcat tcataacaaa
     1501 ttaaggcgtt aaagtttggg gcatctcttc atcagtaaga ggcgggtaag aggcaaaagg
     1561 atcttcaggc tctactacaa tattgtaata gacattgttt tttaattggt cttcaatata
     1621 gaagagcgtt ccacccatag cagaaccaca accagcacag gctccctgaa aacgtatttg
     1681 aaggcgatta tctttagtta aagcaagcac ttctaaatca ccaccatcca tgcgaagggt
     1741 gtcgcgaaca tattcatgaa ggcaatcttc aattttttgg agcttttctt catgagaaag
     1801 agcgttccat tcggcatcgg cttcggcacg gccttcggct gtttgcgttt tatacttaat
     1861 ggcttccatc gcttcacgag cggccagagc cacgtttttc ttttcaggat acacttcttt
     1921 aatgtttaga atcaactttt gtagtaactc gagttctaca gcatcttctg gtagagcttt
     1981 tgtatgggga gtatctctga gtaagctttc aatttctgag atctttatac tagaagcctc
     2041 ttccaccgtt ttattttgaa ttaaagaaca aaacgtatcg gcgagggctg taaaaatagg
     2101 gccaccataa gtgaaaaagc gagtctctaa aataatatcc gaatcggggt ctaccattaa
     2161 ataaacttta agactcgatt ctttaatatc tataagagca aggccctttt catcggcttc
     2221 aatttgaaag atagcaccac ggtatttagg catttttgca gtctgtgcaa gcttattaga
     2281 tatagataaa gattcttcgc tcatagtaag acaaaaatta gaaaaaaatt aaatataaga
     2341 aatccctatt ttaatggagt tcttgttttt attctccact aaaggctcaa aaaacttcta
     2401 ttttttgatt attaagaaga attgacattt attgatgaga gaaggtttta tgagttcact
     2461 aggattagtt cttgaaggcg gtggaatgag gggcgcttat accgcaggtg ttcttgattg
     2521 ttttatgcaa aataaaatag aagtcgatgg cgttgttggg gtatcggcag gagccaccca
     2581 tgcctgtaac
//
LOCUS       DAOOBB010000232         2579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_44361_length_2579_cov_4.094295, whole genome shotgun sequence.
ACCESSION   DAOOBB010000232 DAOOBB010000000
VERSION     DAOOBB010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44361_length_2579_cov_4.094295"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PLT31_08655"
     CDS             <1..210
                     /locus_tag="PLT31_08655"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HPW95239.1"
                     /translation="ESACGDGASFVVLADGRELFRSLKMYSRDKQKVSLPMQGVRRLE
                     LVVEEGDNIDCDHAVWANAWLRRVE"
     gene            210..1286
                     /locus_tag="PLT31_08660"
     CDS             210..1286
                     /locus_tag="PLT31_08660"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPW95240.1"
                     /translation="MKVLVAPLDWGLGHATRCIPLIKALLASGAEVELATCGPMVPLY
                     KEVFPNLRQRRAPSYNVVYPKIGLTMPFWLMKNMAYFSKVFKLEHRWVESLVKRYGYD
                     LIISDNRFGCYSNRVKSIYMTHQLRIGFPKGLHNFENIGIHWHLAQMKNFSEVWIPDF
                     EEFPGLAGKLSHVKKMPPKFRYVGLLSRFQSSTEVFPKEVDILGIISGVEPMRSQFEK
                     KLKQALQKIPGNHVLLLGHPGSSIKKETFGNVTIYNHLDTAAFTAMVQKSKYVVARSG
                     YSTIMDMAVLGASCLFIPTPGQTEQVYLAKSLEESNNAVVLSDLLLNAITIKSALTKV
                     HQIPTPIQNDLSLLNKAIDAVLTK"
     gene            1295..1960
                     /locus_tag="PLT31_08665"
     CDS             1295..1960
                     /locus_tag="PLT31_08665"
                     /inference="COORDINATES: protein motif:HMM:NF025446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4261 domain-containing protein"
                     /protein_id="HPW95241.1"
                     /translation="MNTFILVFPFSEENESLKEGPFMRPGCSVSLIGKKQPAVNGVAE
                     IFSEHALLAKEEFEAIQNHKLLYSIQGEFKTEEEFNDLNQAIASILKSGALGVYMEHS
                     GAAWSKASFLEWVEHEEMMSAWLNFIETNDDLYTLGMESFGRPDLCIRLHHGEKMDLQ
                     GTLVEVAESLFLEESVFETGATIELESTSIRYHIRKEPYQPYRKDDPEYNRKGVWRLV
                     PKK"
     gene            1968..>2579
                     /locus_tag="PLT31_08670"
     CDS             1968..>2579
                     /locus_tag="PLT31_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /protein_id="HPW95242.1"
                     /translation="MKSYILRRLLLMIPTLIGISLVCFILIQMIPGGPVEEMIAKVQS
                     ASAMKGGADASKALSAEQIAQIEAYFGFDKPAWKRYIEWLGNVLTLDLGTSYTYGEPV
                     WDVIVSRFPISLFFGLTSFFLSYLICIPLGLLKAVKHGSRFDVISSGVIFSGYVMPGY
                     ALGILLIIFLAGGSYFDIFPIGGLTSDYFEEMSFFGKVQDLLHH"
BASE COUNT          698 a          444 c          566 g          871 t
ORIGIN      
        1 gaaagcgctt gtggcgatgg tgcttcgttt gtggttcttg cagatggtcg cgaattattc
       61 cgttccttaa aaatgtattc tagagataaa caaaaagtga gccttcctat gcagggcgtg
      121 cgtcgtttag aacttgttgt agaagaaggg gacaacatcg actgtgatca tgctgtctgg
      181 gcgaatgcat ggcttaggcg ggtggaataa tgaaagttct tgtagctcct ttagattggg
      241 gtttaggtca tgccacgcga tgcattcctt tgattaaggc attacttgca agtggtgctg
      301 aagtagagct tgccacttgt gggcccatgg ttcccctcta taaagaagta ttccctaatt
      361 tacgtcaaag acgagctcct tcttataatg tcgtctatcc aaaaattggt ttgactatgc
      421 ctttttggct catgaaaaac atggcttatt tttcgaaggt gtttaagtta gaacatcgtt
      481 gggtagagtc tcttgtgaag cgctatggtt atgatttgat tatttccgac aatcgttttg
      541 gctgctattc aaatcgagta aaatcaattt acatgacgca tcagctgaga atcggttttc
      601 ctaaaggtct tcataacttt gaaaatattg gtattcattg gcatctagct cagatgaaaa
      661 atttctcaga agtttggatt cctgattttg aagaatttcc tggtcttgct ggtaagcttt
      721 ctcatgtgaa gaagatgcct cctaaatttc gttatgtggg cttattgtct cgctttcaaa
      781 gttctactga agtattccca aaagaagtcg atatcttagg cattatttct ggggtagaac
      841 cgatgcgttc tcagtttgaa aagaaattaa aacaggcgtt gcaaaaaatc ccagggaacc
      901 atgttttatt gcttggccat ccagggagtt ctataaaaaa agaaactttt ggaaatgtca
      961 ctatctataa tcatttagat acagcggctt ttaccgctat ggtacaaaaa tcaaaatatg
     1021 tggtagcacg ttctggttat agtacaatta tggatatggc agtattaggc gccagttgtc
     1081 tttttattcc tactcctggg caaacagaac aggtttattt agcaaagagt cttgaagaaa
     1141 gtaataatgc ggtggtgctt tcagatcttc tcttaaatgc aattactata aaatcagctc
     1201 taacaaaagt gcatcaaatt ccgactccca tacagaatga tttgtctttg cttaataaag
     1261 cgattgatgc tgttttaacc aagtaggtct ttttatgaat acatttattt tagtctttcc
     1321 tttttcggaa gaaaacgaat ccttaaaaga aggacctttt atgaggcctg gatgttctgt
     1381 atctttaatt ggtaaaaagc aacctgctgt gaatggtgtt gctgaaattt tttcagaaca
     1441 tgctctctta gcaaaagaag agtttgaggc tattcaaaat cataagcttc tctattctat
     1501 acaaggggag tttaaaacag aagaagaatt caatgattta aatcaagcca ttgcttctat
     1561 cttaaaatcg ggcgctcttg gtgtttatat ggagcactca ggagccgctt ggtctaaagc
     1621 tagtttctta gaatgggtcg agcatgaaga aatgatgtct gcttggctta attttattga
     1681 aaccaatgat gatttgtata ctttagggat ggaatcgttt ggccgtccag atctttgtat
     1741 ccgcttgcat catggagaaa agatggatct acaaggtact ttagttgaag tggctgaatc
     1801 actatttttg gaagagtctg tctttgaaac gggggctaca atcgaattag aatcgacttc
     1861 gatacgctac catatacgaa aagaaccata ccaaccttat cgtaaagacg atccagagta
     1921 taatcgtaag ggcgtgtggc gtttagtgcc taagaaatag aggtttcatg aagtcttata
     1981 ttctgcgccg cttgcttttg atgattccta ctttaatagg gatttcatta gtttgcttta
     2041 ttttaattca aatgatccca ggcggccctg tagaagaaat gattgcgaag gtgcaatcgg
     2101 cttcggcgat gaaaggtggt gcggatgctt ccaaagcgct ttctgcagaa caaattgctc
     2161 aaattgaagc ttattttggt tttgataaac cagcctggaa acgttacata gaatggctcg
     2221 gcaacgtact gacgcttgat ttaggaactt cttatactta tggcgaaccc gtttgggatg
     2281 tgattgtgag ccggtttcct atctctctgt tttttggcct tacttctttt ttcttatcgt
     2341 atttaatatg cattccactg gggctattaa aagccgtaaa acatggcagc cgttttgatg
     2401 tcatttcttc gggcgttatt ttttcgggtt atgtgatgcc aggttatgcg ctcggtatct
     2461 tgcttatcat ctttttagct ggtggttctt attttgatat tttccctatt ggtgggctta
     2521 cgtctgatta ttttgaagag atgtcttttt ttggaaaagt ccaagatcta ttgcatcat
//
LOCUS       DAOOBB010000233         2557 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_44846_length_2557_cov_5.341327, whole genome shotgun sequence.
ACCESSION   DAOOBB010000233 DAOOBB010000000
VERSION     DAOOBB010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44846_length_2557_cov_5.341327"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..603
                     /locus_tag="PLT31_08675"
     CDS             43..603
                     /locus_tag="PLT31_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95243.1"
                     /translation="MNTNTVKIPGPFGDYNHGTVGAILNAATRYENVPLGKVERFQVG
                     DGVITDGVNTIARYKWFDGLDRPCFFWLGEYRRYSNFRMQDRDGKQVPKFRAKLTCHE
                     MAGLEVNGELATPALHQFFKRMLTNYTNDPCYALHKACSAFFQGGSQDANGKWFLLEF
                     WKPEGAQAFIDYLNENYDPEAEVFQF"
     gene            640..1020
                     /locus_tag="PLT31_08680"
     CDS             640..1020
                     /locus_tag="PLT31_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95244.1"
                     /translation="MNKNSSAFDFSINMDNILPELKKNLEERFYLTAQEQIRNRLRIL
                     CMGPKEIDRCKSLFSGKADPDLGMAYNQIQSAIDDVVLSANYKEMAKKYIEENFEKKL
                     IEAMEKAMEHRANKMAFQTVKKWF"
     gene            1255..1497
                     /locus_tag="PLT31_08685"
     CDS             1255..1497
                     /locus_tag="PLT31_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95245.1"
                     /translation="MPSNESSVKLVTQIKEQVAEMETQLNGLQIPEAVLKHINAMLGD
                     VLEHTEAAVALIKIGMHEAALIQLKGYVEPPSILPG"
     gene            1726..2079
                     /locus_tag="PLT31_08690"
     CDS             1726..2079
                     /locus_tag="PLT31_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95246.1"
                     /translation="MIDNNPGYFNFPVIIHPNAVLGECHAVAAHLALHNPTWKLCLGL
                     AEWGECVTEWGMHSWCIDDKNQIIEPTTLIRNRYVGVVIPENFKSSLIWEELSNIQRV
                     LGVDDFELFKNTLSN"
     gene            2095..2538
                     /locus_tag="PLT31_08695"
     CDS             2095..2538
                     /locus_tag="PLT31_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95247.1"
                     /translation="MRKIFFDLEGTLINSWSDVSLCNIQRVRNIIKEHGITEISIFSF
                     AIDNENDVRIFEKDGFKNFIERTYEIKIVEYPTTKEIMSVCLEYSGNLFDLFEFKSVW
                     GKTRAFQEYCTALYKDTACTLIDDVVPNTTLYMHDKGLTISTIKI"
BASE COUNT          849 a          419 c          520 g          769 t
ORIGIN      
        1 gatgagattt ttgaaatcct taacatggtg tggtgaataa aaatgaatac taataccgta
       61 aaaattcctg gtccttttgg cgactataat catggcactg ttggtgctat tctgaatgca
      121 gccacgcgat atgaaaacgt tcctttgggt aaggttgagc gttttcaagt aggtgatggt
      181 gtcattactg acggtgttaa taccattgct cgctacaagt ggtttgatgg tcttgatcgt
      241 ccttgctttt tttggctggg agaatatcga aggtatagta atttccgaat gcaagatcgt
      301 gatggaaaac aagttcctaa gtttcgagca aaactgactt gccatgaaat ggctggcctt
      361 gaagtaaatg gagagctggc aacgcctgca cttcatcaat tcttcaaaag gatgctaacc
      421 aactacacca atgatccttg ctatgccctt cacaaggctt gtagtgcatt ctttcaaggt
      481 ggtagtcagg atgctaacgg taagtggttt ttgcttgaat tctggaagcc tgagggtgct
      541 caagcattca tcgattacct caatgaaaat tacgatcctg aagcagaagt ttttcaattc
      601 taaacacaca tacttttaaa atacaaggaa aaaacaatca tgaataaaaa ttctagtgct
      661 tttgattttt caatcaatat ggacaacatt ctgcctgagc ttaagaaaaa tctggaagag
      721 cgtttttatt taactgcaca agaacaaatt cggaaccggc tgcgcattct ttgcatgggt
      781 cctaaagaga ttgatcgctg taaatctctg tttagtggaa aagctgatcc tgatttggga
      841 atggcgtata accagattca atcggcaatt gacgatgttg ttcttagcgc caattacaaa
      901 gaaatggcta agaagtacat agaagaaaac tttgaaaaga agctcataga agctatggaa
      961 aaagccatgg agcaccgagc aaataagatg gcttttcaga ctgtgaagaa gtggttttaa
     1021 gaatacggta gtaggtgaat tcctgaggaa cacattaccg atgaagaact tgaagaggct
     1081 ctaaaattta cttatgtaga agaagattgg gataaaggag gaccgtgtaa aactctttac
     1141 gaacttggta actcttttgc aagtaaagaa gatgcacaaa aaaccatcaa tcgaattctg
     1201 actgcactta aaagaattcg taactaatac tgaaaataaa caaggacaat caaaatgcct
     1261 tcaaacgaaa gctccgttaa gcttgttacc caaatcaaag aacaagttgc tgaaatggaa
     1321 acacaactta acgggcttca aattcctgaa gcagttctta aacacattaa tgcaatgctt
     1381 ggcgatgttc ttgaacacac cgaggcagct gttgctttga ttaaaatcgg tatgcacgaa
     1441 gcggctttga ttcaattgaa aggctatgta gaacctccct ctattttgcc tggttaatgg
     1501 ttataaatta gagagtagtg gatcatgatc cactactctc taataaagaa gtacatgaat
     1561 ttgatttaaa tgaagtaaac tcttttatca atacgttttt aaagcacttt attttttaat
     1621 aagaaagcca aaatgccgta tctaaaacaa cttctagata agtttaatat aagtagtgag
     1681 gatatgtgtt ttacttcgca cattcgcaaa caagatgcat ggctaatgat tgataacaat
     1741 ccagggtatt ttaatttccc tgttattatt catcctaatg cggtgctagg agaatgtcat
     1801 gcagtggctg ctcacttggc gttacacaat cctacatgga agttgtgtct tggtttagct
     1861 gaatggggag aatgtgtgac tgagtggggg atgcactcgt ggtgtattga cgataagaac
     1921 caaatcattg agcccaccac acttatacgt aaccggtacg taggagtggt tataccagaa
     1981 aattttaaaa gttctcttat ttgggaagaa ctctcaaaca ttcagcgggt actaggtgtg
     2041 gatgattttg aattattcaa aaacactctt tcaaattaaa tataaaatac ttttatgaga
     2101 aaaattttct ttgatctaga aggcacgctg attaactctt ggtcagatgt gtcgctttgc
     2161 aatattcaaa gagtgagaaa cattattaaa gaacacggta ttactgaaat tagtattttc
     2221 agttttgcta ttgataatga aaatgatgtt cgtatatttg aaaaagatgg atttaaaaac
     2281 tttattgaga gaacatatga aatcaaaata gttgaatacc caactacaaa agagatcatg
     2341 tctgtttgtc ttgaatacag tggaaatctt tttgatctct ttgagtttaa gtctgtctgg
     2401 ggaaagacaa gggcatttca ggaatactgc actgcactct acaaagatac agcatgtacg
     2461 ttgattgatg atgttgttcc taacacaaca ctttacatgc acgataaagg attgactatt
     2521 tcaactatta aaatttaaaa gtaaaaaata tttaatc
//
LOCUS       DAOOBB010000234         2536 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_45313_length_2536_cov_3.616687, whole genome shotgun sequence.
ACCESSION   DAOOBB010000234 DAOOBB010000000
VERSION     DAOOBB010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45313_length_2536_cov_3.616687"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..689
                     /locus_tag="PLT31_08700"
     CDS             123..689
                     /locus_tag="PLT31_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HPW95248.1"
                     /translation="MFLTRKAITEAVECGDIVIEPFHPENLSPNSIDVTLSENLLTYT
                     LEGGCLDMKKENPTEHLIIPKEGMVLQPGILYIGMTNESAISHRYIPMFEGRSSIGRL
                     GINTHITAGFGDVGWGFEKSENGEIICHYPTWTLEIAVVHPVRVYPGVRIGQVYFVEP
                     KGDVEWYKGKYSKQRKPQPSCAYKDFTA"
     gene            707..1369
                     /locus_tag="PLT31_08705"
     CDS             707..1369
                     /locus_tag="PLT31_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95249.1"
                     /translation="MFFFVACTSSHQWSAVHPSYKKGISSNDAIGIVGTQNAFILSRA
                     KWFSNRLEIPMDSIHSVVDSLMTSNFTNEFKNYHSQVLFIPDSVKKNWPTESQKLDKS
                     IFLKGQLPAQGIVIKNDTHTPPYLFILHEFVIGTDLDRSLYYDYTQGGQEVEVKTSVK
                     HVTAILSYTLWDNLKQIPLFSSVIEIQEPYTNKLTETYLKQITQKAVKEIHQEISRRK
                     SP"
     gene            1366..>2536
                     /locus_tag="PLT31_08710"
     CDS             1366..>2536
                     /locus_tag="PLT31_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95250.1"
                     /translation="MKKLALFFFLFFYVVDLSAKETVFNNDYTIWKDADILIWTKDSN
                     LVISAKELCFSLKRINPTIGEPKCRIFGEWERDSVASLYGHWLKSNLSPDMTAEHLRA
                     RSPEMVQKINDLEDKTVIFLTQKENQLYVALFEELSASPKTVQILPYSNNQIEQGEII
                     ANALFKKKADRRLTKDERKKKAEEPDSYYLETAKHHSWIGVAYGKTQAPLPLTPKSWY
                     KNKLNSRIKSYRNTKDSLSLWNFIEDEAPLTSIYFGRTWYGFIGAEFFYRYSSHKAKI
                     DNTDPIYQELDYWKFSRHEIGLSLVFSKNYQSFSFLQTVPYAFIGFQYSFFNEDISLK
                     KGIETPSRQYETRIKFENAYKGALIGAGTHLIFFKHYGINLKAGIGSRGRMLDSDP"
BASE COUNT          845 a          503 c          450 g          738 t
ORIGIN      
        1 attattaata ttaacgatat tattgagttc ctcacaaaag aagaaaatcg aaaaaaaata
       61 aatgctccag aaaatatttt agagcgtgta tacaattatc gaaacgaatg gggtgtagcc
      121 taatgttttt aactagaaaa gccattactg aagccgtaga atgtggcgat atcgtcattg
      181 aaccttttca tcctgagaat ctttctccta atagtattga tgttacctta agtgaaaact
      241 tgttaacata cactcttgaa ggtggctgtc ttgacatgaa aaaagaaaac cccaccgaac
      301 atttaattat tccaaaagaa ggcatggttt tacaaccagg aattctatat attggaatga
      361 ctaatgaatc tgcgattagc catcgttata ttccaatgtt tgaagggcgc agttccattg
      421 gtcgcttagg gattaacacg cacattaccg ctgggtttgg tgatgtgggt tggggttttg
      481 aaaaaagtga aaatggtgaa atcatctgcc attaccccac ttggacttta gaaattgcag
      541 ttgttcatcc tgttagagtt tatccaggtg ttcgaatcgg ccaggtttat tttgtggaac
      601 ctaaaggaga cgttgaatgg tacaaaggaa agtacagcaa acaacgaaag cctcaaccaa
      661 gctgcgccta caaagacttt acagcataaa ggctctcctt ttttgtttgt tcttttttgt
      721 tgcttgtact tcatcacacc aatggagtgc agttcatccg tcttataaaa aaggaatctc
      781 ttccaatgac gctattggca tcgttggtac acaaaatgcc tttattttaa gccgtgccaa
      841 atggttttct aatcgtctcg aaattccaat ggatagtatt cattcagttg tcgattcttt
      901 aatgacctct aatttcacga atgaatttaa gaactaccat tcacaagtcc tctttatccc
      961 agactctgtc aaaaaaaact ggccgactga aagtcaaaag ctagataaat ctattttcct
     1021 caaagggcaa ttaccagcac aagggattgt catcaaaaat gatactcaca cgccccctta
     1081 cttatttatt cttcatgaat ttgtgatagg cacagatcta gatcgaagtc tttattatga
     1141 ttatactcaa ggcgggcaag aagtagaggt aaaaacgtca gtgaagcacg tgaccgccat
     1201 tctttcttac actttatggg ataacctaaa acaaattcct cttttctcct ctgtgattga
     1261 aattcaagag ccttatacaa acaaattaac cgaaacgtat ttaaaacaaa tcacgcaaaa
     1321 agcagtgaag gagatacatc aagaaatttc acggaggaaa tcaccgtgaa aaaattagcc
     1381 ctcttcttct ttttattctt ttatgtagtg gatttatctg ctaaagaaac ggttttcaat
     1441 aacgattata cgatctggaa agatgccgat attttaatct ggactaaaga ctctaattta
     1501 gtgatctctg caaaagaatt atgcttttcg ctaaagagaa tcaatccaac cattggcgaa
     1561 cctaaatgcc gtatctttgg tgaatgggaa cgtgactcgg tggcctcctt atatggccat
     1621 tggctaaaaa gcaatttaag tcccgacatg acagccgaac atctccgtgc gagaagccct
     1681 gaaatggttc agaaaataaa cgacctagaa gacaagacgg ttattttcct cacacaaaaa
     1741 gaaaatcagc tttacgttgc tttatttgaa gaattatcag cctctccaaa aacagtacaa
     1801 atcctacctt attcaaacaa ccaaatcgaa caaggtgaga tcatcgcaaa cgccttattt
     1861 aaaaaaaaag cggaccgacg tctcactaaa gacgaacgga agaaaaaagc agaagagcct
     1921 gattcttatt atttagaaac agcgaaacac cattcttgga ttggtgttgc ctacggaaaa
     1981 acacaagccc ctcttccact cacgccaaaa agctggtata aaaacaaact caatagtcga
     2041 attaaaagct atcgaaatac aaaagattct ttaagcctat ggaattttat tgaagacgaa
     2101 gctcctctta cctctatata ttttggaaga acttggtatg gttttatcgg agctgaattc
     2161 ttttatcggt attcctctca caaagcgaaa atcgataaca ccgaccctat ttatcaagaa
     2221 ttagattatt ggaaattcag caggcacgaa attggcctca gcctcgtttt ttcaaaaaac
     2281 taccaatcct tttcttttct tcaaactgtc ccttacgcct ttattggatt tcaatacagc
     2341 ttttttaatg aagacatctc tctaaaaaaa ggcatcgaaa caccctctcg tcaatatgaa
     2401 acacgcatta aatttgaaaa tgcatataag ggagctctaa ttggagcagg gactcattta
     2461 atctttttca aacactatgg aatcaattta aaagcaggca tagggagtcg tgggcgcatg
     2521 cttgatagcg atccct
//
LOCUS       DAOOBB010000235         2535 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_45349_length_2535_cov_3.437097, whole genome shotgun sequence.
ACCESSION   DAOOBB010000235 DAOOBB010000000
VERSION     DAOOBB010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45349_length_2535_cov_3.437097"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /locus_tag="PLT31_08715"
     CDS             complement(<1..1095)
                     /locus_tag="PLT31_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HPW95251.1"
                     /translation="MITPMKKVTILCIDSLKTKTLEELRSMGLLHVIHLQSPTGSDLN
                     YAKAELQRVQKAIDVVPSKVKKEVAPITGSPDSLISELQNLIAERKEDEDTLAQMKVD
                     LARYDVFGDFNPSQIEALAEKGIFIRLFEAEKNKKPLPDDESALVQYYGKNSVADCFV
                     VINRGEKPADVKMANELRLPSLSLSEMKSKLSYAEKSLKKIESRLQEIATVKSSLKEQ
                     LLDVSDDYNLKEVNAGMIQNSGIAMIQGFCPEPRLAELKAMAPKLGWGLKIEDPSEEE
                     FVPTLLSYNKASRPMKFLYDIIGISPGYHEMDVSLVFLAFFSVFFAMIVGDTVYGILF
                     LIITLIAQAKLPKAPKAAFHFLILMSCTTII"
     gene            complement(1092..1715)
                     /locus_tag="PLT31_08720"
     CDS             complement(1092..1715)
                     /locus_tag="PLT31_08720"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014544951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HPW95252.1"
                     /translation="MAKVKLTKNALKAERDALKRFKRYLPTLLLKKQQLQMEMRTLQA
                     KVIVKRKEEDALRNMMSDWVCLYADPVDWSSYLSVKEVREGEGNIAGVEIPLFNGVDF
                     NLSIPDPFDTPVWLDDGIRALQSLISLRLERRVLEKQYELLSEELRTTSQRVNLFEKV
                     KIPEAKENIRAINIFLGDQQTTGVARSKLAKGKSVARTLLEDGGVAA"
     gene            complement(1720..>2535)
                     /locus_tag="PLT31_08725"
     CDS             complement(1720..>2535)
                     /locus_tag="PLT31_08725"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014544950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HPW95253.1"
                     /translation="LQAEVDVIVLGGMGLKHDDYLYLRDFLEQNGALSRSVMFVHTAS
                     DPIVECLLVPDAALAVAEQFAIKGKNVLVLLTDMTNFADAMKEIAITMEQIPSNRGYP
                     GDLYSQLASRYEKAVDFDTAGSITILAVTTMPGDDVTHPVPDNTGYITEGQFYLRKGR
                     IEPFGSLSRLKQQVNGSTRSDHRTIMNTMIQLYASFKETIEKQSMGFRMSTWDQKLLR
                     YGVRFEKEMMDLSVNIPLEKALDLGWIILSDCFEPEETGIPSKMINEYWPKKG"
BASE COUNT          802 a          516 c          436 g          781 t
ORIGIN      
        1 gatgattgtc gtacagctca ttaaaattaa aaaatggaag gctgcttttg gagctttagg
       61 gagttttgct tgagcaatta aggtgatgat gaggaataaa ataccataaa cggtatcacc
      121 cacaatcatc gcaaaaaata cgctaaagaa agcaaggaaa acaagagaca cgtccatttc
      181 atgataacca ggagaaatcc cgataatatc ataaaggaat ttcattggac gagatgcttt
      241 attataagaa agtaacgttg gaacaaattc ttcttctgaa gggtcttcga tctttaagcc
      301 ccaacctaat tttggagcca tcgcttttaa ttcagcaaga cgcggttctg gacaaaatcc
      361 ttgaatcata gcgattcctg aattttgaat catcccagca ttcacttctt ttaagttata
      421 gtcatcagaa acatctagca actgttcttt taaggaactc tttactgttg caatttcttg
      481 aagacgagat tctatttttt tcaaagactt ttcagcataa gaaagctttg atttcatttc
      541 actcaaagaa agtgaaggta aacgaagttc attagccatt tttacatctg caggcttttc
      601 acctcgatta ataaccacaa agcaatccgc tacagagttt tttccataat attgaaccaa
      661 agcagattca tcgtctggaa gaggcttttt atttttttct gcttcaaaaa gacgaataaa
      721 gatgcctttt tcggctaaag cttctatttg agatggatta aaatcaccaa aaacatcata
      781 tcgagcaaga tccactttca tttgtgcaag tgtatcttcg tcttctttac gttctgcaat
      841 tagattttgt aattcactta taagagaatc aggagaacct gtaattggag cgacctcttt
      901 ttttacctta gagggaacca catctattgc tttttgaaca cgttgtaatt ctgcttttgc
      961 ataattaaga tcagaacccg taggcgattg caaatgaatc acatgcaaca gacccataga
     1021 acgcaattct tcgagagtct ttgtttttaa agaatctata cagagaatcg ttaccttttt
     1081 cattggagta atcatgctgc gactcctcca tcttccaata atgtacgagc aacggactta
     1141 cccttggcaa gtttagaacg agccacacct gtcgtttgct gatcaccaag aaagatatta
     1201 attgcacgaa tattttcttt cgcttctgga attttcactt tttcaaaaag attcacacgt
     1261 tgacttgtcg tacgtaattc ttctgaaagt aactcatatt gcttttctaa aactcgcctt
     1321 tctaaacgaa gagaaattaa agattgcaat gcacgaatac catcatctag ccacacgggt
     1381 gtatcaaatg gatcaggaat actaagatta aaatcaacac cattaaacaa aggaatttct
     1441 acaccagcaa tgttaccttc accttctctc acttctttaa cggaaagata agaactccag
     1501 tctactggat ctgcatataa acaaacccaa tcggacatca tgttgcgaag agcatcttct
     1561 tcttttcgtt ttacaatcac tttggcttgc aaagtacgca tttccatttg caattgttgc
     1621 ttttttaaca gaagtgtagg caaataacgc ttaaatcgct taagcgcatc acgctctgct
     1681 ttcaaagcat ttttggtgag tttcactttt gccatagcgc tacccttttt ttggccaata
     1741 ttcgttaatc atcttagaag gaataccagt ttcttctggc tcgaaacaat ccgaaagaat
     1801 aatccaacca agatctaaag ctttttctaa tgggatattt actgaaaggt ccatcatttc
     1861 tttttcaaaa cgaacaccgt atctcaataa tttttgatcc caagtgctca ttctaaagcc
     1921 catcgattgc ttttcaatgg tttctttaaa gcttgcatac aattgaatca ttgtgttcat
     1981 gatggtacgg tgatcagaac gtgtagaacc attcacttgc tgtttcaaac gagatagaga
     2041 accaaatggt tcaattcttc ctttacggag atagaactgc ccttctgtaa tgtaacctgt
     2101 attatctgga actggatgag tcacgtcatc gccaggcata gttgttacag caagaatggt
     2161 aatggaacca gcagtatcaa agtcaacagc tttttcataa cgacttgcta actgagaata
     2221 aagatcacca ggataaccac gattagaagg gatttgctcc atcgtaattg caatttcctt
     2281 catggcatcg gcaaagtttg tcatatctgt taacaaaaca aggacgtttt tacccttaat
     2341 tgcaaattgt tctgcgacag ctaaagcggc atctggaaca agcaagcatt ctacgatagg
     2401 atcagaagct gtatggacaa acatcacaga gcgagaaaga gctccatttt gctccaaaaa
     2461 gtctctaaga taaaggtagt cgtcgtgttt taagcccata ccgccaagaa caataacatc
     2521 cacttcagct tgaag
//
LOCUS       DAOOBB010000236         2522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_45636_length_2522_cov_4.476692, whole genome shotgun sequence.
ACCESSION   DAOOBB010000236 DAOOBB010000000
VERSION     DAOOBB010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45636_length_2522_cov_4.476692"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..1080)
                     /locus_tag="PLT31_08730"
     CDS             complement(127..1080)
                     /locus_tag="PLT31_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW95254.1"
                     /translation="MNKPVLSLEEVSVSFGSKDPIAVTDRVSFEIFPGEFFALVGESG
                     CGKSVTAMSILRLLPTPSAKIISGRILFDDRDLTRLTDAEIRRIRGSSISCIFQEPMQ
                     ALNPVLTIGKQLLEVLRHEKKDAAMDRIKKYLKLAGLSDIDRIWKAYPHEMSGGMLQR
                     ICIVMALLPSPSLIIADEPTTALDVTVQAQIMDVLKRLSQESNTAVLLITHNMGLVAQ
                     YACRVGVMYAGRLVEMGPVDQVIHSPKHPYTKGLLAAIPEGHTGVQSITSIPGNVPDP
                     KDFVEGCRFSNRCSFYKKECDAYPPKQGNDEHYAYCIKTDN"
     gene            complement(1073..2098)
                     /locus_tag="PLT31_08735"
     CDS             complement(1073..2098)
                     /locus_tag="PLT31_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPW95255.1"
                     /translation="MRISQETKNKWIRFRKNKRAFYSLVILVVAYLASLTSPWTVNDK
                     PLVMLFNDNFYFPAFFHYSETEFGGVYNTEPDYKKLLYSVDADIWVIMPPIPHDPLKA
                     DLSLEGTPPFPPSASHLLGTDANGRDILSRLIHGFRICLSFSLCLTVLGTLLGILIGG
                     IQGYLGRYFDTMFQRGIEIWSSLPFLYVVILIGSIYGRSFLLLILIMAAFNWISLSYY
                     MRAEFLKLRSMPYVKAASMLGLSHRHLFFKEILPNALTPVVTLFPFTLIGGIGSLTSL
                     DFLGFGLQPPTPSWGELMSQGLNHLYAPWISISTVAALFITLLLATFIGEGVRDAMDP
                     KSGDRYE"
     gene            complement(2105..>2522)
                     /locus_tag="PLT31_08740"
     CDS             complement(2105..>2522)
                     /locus_tag="PLT31_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPW95256.1"
                     /translation="LLHHLVLPMFCYMISEFAFLTFLMKNSALEELGRDYMRTALAKG
                     LSFREAVFKHALRNALIPVATRLSEIFTLMFAGALLIEKVFDIDGMGLLYYNSMVNRD
                     YNVVLGIIFLSSFMAMIGRLFSDILYTFVDPRIKFS"
BASE COUNT          856 a          542 c          477 g          647 t
ORIGIN      
        1 cttctttagg cttgcgaaaa aactttccat ggctgcgtta tcatacgggc agccaaatac
       61 tcattagcgc aatggtcact aataattcaa ttaacgtaaa tcctattttt ctgattattt
      121 tgatgatcaa ttatccgttt taatacaata agcgtaatgc tcgtcattgc cttgctttgg
      181 cgggtaagca tcgcattctt ttttatagaa agagcatcga ttactaaaac gacagccctc
      241 tacaaaatct ttagggtcag gaacatttcc tggaatagat gtaatcgatt gcaccccagt
      301 atggccttct ggaatagcgg ctaaaagtcc cttggtgtaa gggtgcttag gcgaatgaat
      361 cacttgatca actggtccca tttccacaag acgcccagca tacatcaccc caacacggca
      421 agcgtattgc gcgactaaac ccatattatg agtaatcaat aaaacggcgg tattagattc
      481 ttgagaaagg cgttttaata catccattat ttgggcttgt acagtcacat ctaaagcagt
      541 cgtcggttca tctgcaataa tcaagctcgg cgaagggagt aacgccatca caatacaaat
      601 acgctgtaac atgcccccag acatttcatg agggtaagct ttccaaatac gatcaatatc
      661 agaaagccct gccagtttca agtatttttt gatgcgatcc atagccgcgt cttttttctc
      721 atgacgaagc acttctaaaa gttgcttccc tattgtcaat actggattta aggcctgcat
      781 cggttcttga aaaatacaag aaatcgaaga gcctctgatg cgtctgattt ctgcatctgt
      841 taagcgagtc aaatcacgat catcaaaaag gatacggcca gaaataattt tagcacttgg
      901 cgttgggagt aatcgaagaa tactcatcgc tgttacactt tttccacaac cagactcccc
      961 cacaagcgca aaaaattctc caggaaaaat ttcaaatgaa actcgatctg taacagcgat
     1021 aggatctttt gaaccaaaag aaacagaaac ttcttctaga gataaaaccg gtttattcat
     1081 aacgatctcc cgattttgga tccattgcat ctcgaacgcc ttctcctata aaagtcgcga
     1141 gcaacaaagt gataaaaaga gccgccaccg tactaatgga aatccaaggc gcatacaagt
     1201 gatttaatcc ttggctcatt aactctcccc agcttggcgt aggtggctgt aaaccaaagc
     1261 ctaagaagtc gagagacgtt aaactaccaa ttccaccgat taacgtaaac gggaaaagag
     1321 tcactacagg cgttaatgca tttgggagaa tttctttgaa aaataaatgg cgatgagaaa
     1381 gcccaagcat actagcggct ttcacataag gcatggagcg taattttaag aactccgctc
     1441 gcatataata acttaaagaa atccaattaa atgcagccat gattaatatt aaaagcaaaa
     1501 agctacggcc ataaatactt ccaatgagaa tcactacata taaaaacgga agagaagacc
     1561 atatttcaat gccacgttga aacatcgtat caaaatagcg ccctaaatag ccttgtatgc
     1621 cgccaatcaa aatgccaaga agcgtgccaa gtacagtgag gcacaaacta aaagaaaggc
     1681 aaatacgaaa tccgtgaatc aagcgagata aaatatctcg gccattggca tcggtaccga
     1741 gaaggtgcga cgcagatgga ggaaaaggag gtgtgccttc taaagaaaga tctgctttca
     1801 atgggtcatg aggaatcgga ggcataatca cccaaatatc tgcatctaca gaataaagca
     1861 gctttttata atcgggttct gtattgtaaa cgccaccaaa ttctgtttct gaataatgaa
     1921 aaaaggctgg gaaatagaaa ttatcattaa aaagcatcac caagggttta tcgttcaccg
     1981 tccaagggct tgtaagagat gctaaataag ctaccactaa aatgactaag gaataaaacg
     2041 cccgtttgtt ctttcggaat cgtatccact tattttttgt ttcttgactt attctcataa
     2101 agcctcaact aaacttgatg cgtggatcga caaacgtata aagaatatca ctaaacaaac
     2161 gcccaatcat cgccataaaa ctagagagaa agataatccc aagaaccaca ttataatctc
     2221 gattgaccat ggaattataa tagagaagcc ccatgccatc aatatcaaac actttttcga
     2281 ttaaaagagc ccctgcaaac atcaaagtga aaatctctga aagcctcgtt gctacaggga
     2341 ttagcgcatt gcgcagagca tgcttaaaca ctgcttcacg gaaagaaagc ccttttgcta
     2401 aagcggttcg catgtagtca cggccaagct cttctaacgc actatttttc attaagaagg
     2461 ttaaaaaagc aaattcgcta atcatatagc agaacattgg taaaacgaga tgatgcaata
     2521 ga
//
LOCUS       DAOOBB010000237         2505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_46050_length_2505_cov_4.300816, whole genome shotgun sequence.
ACCESSION   DAOOBB010000237 DAOOBB010000000
VERSION     DAOOBB010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46050_length_2505_cov_4.300816"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="PLT31_08745"
     CDS             <1..957
                     /locus_tag="PLT31_08745"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPW95257.1"
                     /translation="HANMRLDRYLRQSFSTESLSALFAVIRKKKVRVNGVVAKAPLIL
                     NEGDLVAIYENLKEDNSTSAPKTGWGNNNTYCDPLFFKNHFQIALETEDFVIVEKPSG
                     IDSQPGSGTRPKETLVDMLWQWGLNEKHDFKPTLVHRLDRETSGLLIAAIQGDVLRGL
                     TKLIREHKINKYYYALVQGHLEKTKGTIKTNLERSDSPQGAKMKTSESKGKESITHYN
                     VYRRFESMDLVKIKLETGRMHQIRAHFASINHPLAGDSRYGDYAWNRELRKEYGLNRL
                     FLHSFKLEFEWNNQKYNVESKLPKELDQIVRTLSKKSIKIED"
     gene            complement(1163..1894)
                     /locus_tag="PLT31_08750"
     CDS             complement(1163..1894)
                     /locus_tag="PLT31_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95258.1"
                     /translation="MNKKYYMIAVIVIIALAACSDEEIVGNSVDPNALAELSERDIYF
                     MDSVLHAYGPTIYLNNSFDDPGLVYNITTPSPVSYSFKTEKEVYYYYLNQGSTDGCSV
                     NIYKDEPGVQKMKFPRGGSNILAILLKEFEGSQFIHYLWSSYYGFERPTGECSADSIA
                     FIANCNSLGGSLASYSEGCITEKLEMICIAKIQEALYLEDLAKPLKEECEKFVDSLPE
                     PEVKPTIICQGTTEGGMLCDTTWQD"
     gene            complement(1887..>2505)
                     /locus_tag="PLT31_08755"
     CDS             complement(1887..>2505)
                     /locus_tag="PLT31_08755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4423 domain-containing protein"
                     /protein_id="HPW95259.1"
                     /translation="FEQMVIFGNAKADKIKKEALLKMEKIAREHKARVIDSDAFEYYG
                     SWMYPVIRELAPMMQGALPKEMADVCHEAVSAEEVRNVLSFLTKTGFLKKTSENHFVQ
                     TEKTVIGSMESLPIAIRAMHNKMAELAVHAVDKYSPEERHFLGVTLGVNDESYGLITE
                     EIEKCCKKIMAIAEEHDNINQVYRLNFQLFPFTKKYKCKKEKGNE"
BASE COUNT          814 a          501 c          404 g          786 t
ORIGIN      
        1 catgccaata tgagacttga ccgttattta cgtcaaagtt tttctacaga atctctttcc
       61 gctttatttg ccgttatcag aaagaaaaag gtaagagtca acggtgttgt cgccaaagct
      121 cctttaattc ttaatgaagg ggatttagtt gctatttatg aaaatttaaa agaagacaat
      181 tccacttctg ctcccaaaac aggatggggg aataacaata cttattgtga tccactcttt
      241 tttaaaaatc attttcaaat tgctttagaa acagaagact ttgtaattgt agagaagcct
      301 tctggaattg atagccaacc tggttctgga actcgtccaa aagaaactct tgtcgatatg
      361 ctctggcaat ggggtctcaa tgaaaagcat gattttaagc caactcttgt gcatcgatta
      421 gatcgtgaga cttctggcct tttgatagcc gccattcaag gtgatgtatt aagaggttta
      481 acaaagctca ttcgagagca taaaatcaac aaatattatt acgctttagt ccaaggccac
      541 ttagaaaaaa caaaaggaac tattaaaacc aatttagagc gttctgattc gccacaaggt
      601 gcaaaaatga aaacgagcga atccaaaggc aaagaaagta taacccatta taatgtctat
      661 cgtcgttttg aatctatgga tttagtcaaa atcaaactag aaacgggaag aatgcaccaa
      721 attcgtgcac actttgcaag tattaatcat cctcttgctg gagattctcg ttatggagat
      781 tatgcttgga atcgagaact acgaaaagaa tacggattaa atcgcctttt tcttcacagt
      841 tttaagcttg aatttgaatg gaataatcaa aaatacaatg tggaatctaa actccctaaa
      901 gaattagatc aaatcgttcg aactttatct aaaaaatcca ttaaaatcga agattaacaa
      961 ttctcataca ataaaaaatg cggcgataaa tcgccgcatc ttaagaacac tctcagattt
     1021 tgtgactaaa aactatatac catttgttag tagtcaaaca agccctgatg ctatttataa
     1081 aatagtagaa acacttctat aaatcaagtt ttgattattt ttctatagta caatccacgt
     1141 tattccaagt tagaatagcc cattaatctt gccaagtcgt atcacaaagc atccctcctt
     1201 ctgttgttcc ctgacaaatg atagttggtt ttacttctgg ttcaggaagg gagtcaacaa
     1261 atttttcaca ttcttctttt aatggtttag ccaaatcctc taaataaaga gcttcttgga
     1321 tcttcgcaat acatatcatt tcaagttttt cggtgataca accttccgaa taagaagcaa
     1381 gagaaccacc caaactatta caattggcaa taaaggcaat actgtcagca gaacattcac
     1441 ccgtaggcct ttcaaagccg taataacttg accataaata atgaataaat tgactccctt
     1501 caaactcctt caataggata gccagtatat ttgatccccc tctaggaaat ttcattttct
     1561 gaacaccagg ttcatctttg tatatgttta cagagcaacc atcggtactg ccctgattca
     1621 aatagtaata ataaacttct ttttctgtct taaaggaata agaaacagga gaaggtgtgg
     1681 taatattata aaccaaccct ggatcatcaa aagaattatt aagataaatc gttggtccgt
     1741 atgcatgtaa tacactatcc ataaaataga tgtctctttc agataattct gccaaagcat
     1801 taggatctac agagttaccg actatttctt catcagagca tgccgctaga gcgattatga
     1861 ctataacggc tatcatataa tatttcttat tcattgccct tctccttctt gcatttatac
     1921 ttttttgtaa acgggaataa ttgaaaattc aaacgataca cttgatttat attgtcgtgt
     1981 tcttcagcga tagccataat cttcttgcag catttttcta tttcctcagt gatcaaacca
     2041 taggattcat cgtttacgcc aagagtgact cctaaaaagt gtctttcctc tggactatat
     2101 ttatctacag catgaacagc caactcagcc attttattgt gcatcgcgcg tatagcaata
     2161 ggcaaagatt ccattgaacc gattaccgtt ttttctgttt gtacaaaatg attttcactt
     2221 gtctttttta agaagcctgt cttagtcaaa aaagaaagta catttcgaac ctcttcggca
     2281 gaaactgcct catggcagac atccgccatc tctttaggta aagctccttg catcatagga
     2341 gcaagttcac gaatcactgg atacatccag cttccgtaat actcaaaggc atcactgtca
     2401 atcacacgtg ccttatgttc cctggcaatc ttttccattt taagcaaagc ttctttcttt
     2461 attttgtctg ctttggcgtt gccaaaaatc accatctgtt caaag
//
LOCUS       DAOOBB010000238         2494 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_46330_length_2494_cov_3.859779, whole genome shotgun sequence.
ACCESSION   DAOOBB010000238 DAOOBB010000000
VERSION     DAOOBB010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46330_length_2494_cov_3.859779"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="PLT31_08760"
     CDS             complement(<1..993)
                     /locus_tag="PLT31_08760"
                     /inference="COORDINATES: protein motif:HMM:NF015791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HPW95260.1"
                     /translation="MNQQKVKLDEIRLPLISASQAIELDRSEKGDSIRKGYTLMEEAG
                     EAIYEKIQLFRTNHPSVAQKPVLIFTGGGNNGGDGFVAARLLLKNKVPYRVYGLVDPI
                     RFKNEAFLAYHDFIQAGGFFSHIQDVDSLEMDSSSGCLVVDALLGIGAVGELRPKFAK
                     LVEKLNFLEIPILSVDSPTIPLKATETIILGNPRREAFDSSLSSFYGKVTVAPLSYQK
                     SLIKEISSGDYWISDSDFLKLLPNRNEYGDKRAQGTGLIISGSIGMTGAPSLAALAAL
                     RSGIGLLSMALPSSILQTLSIKLNEPVFYALEDHQQGSFLKEHLDFIKALFKYQK"
     gene            complement(1297..>2494)
                     /locus_tag="PLT31_08765"
     CDS             complement(1297..>2494)
                     /locus_tag="PLT31_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HPW95261.1"
                     /translation="KKDGVKRVRVHILLDGRDVPETSALDYVEPFEAFLADLCSVDFD
                     ACIASGGGRMQITMDRYGANWKMVEKGWKTHVLGEGRLFDSAKEAIETLRGETKAIDQ
                     DLPPFVIGKNGSPVGTIQDGDAVVFFNFRGDRAIEITRAFEEESFTEFDRVRFPKVVY
                     AGMLQYDGDLKLPNRFLVPPPAIKNTSGEWFSKSGIKQFACSETQKFGHVTYFWNGNR
                     SSKFDGETYLEVPSDVIPFEQRPWMKSAEVTDAMIEALKSGKYQTLRCNYPNGDMVGH
                     TGSFRAATMAIEAVDLALARLLPVIDALGGIALITADHGNADEMYELDKKTHLPKQNK
                     DGSFKSKTAHTLNPVPCILYDNVSGGKLALKSGSWGLSNLAATTANLLGFEKHESWDD
                     SMLTLK"
BASE COUNT          812 a          532 c          422 g          728 t
ORIGIN      
        1 cttttggtat ttaaaaagtg cttttataaa atccaaatgt tcttttaaaa aagagccttg
       61 ttgatggtct tctaacgcat aaaaaacggg ctcatttaac tttatcgaaa gcgtctgtaa
      121 aatagaagaa gggagagcca ttgacaaaag ccctatacca gaacggagtg ctgcaagagc
      181 cgctaaagag ggagcccctg tcattccaat agaacctgag atgatgagcc ctgttccttg
      241 agcacgctta tctccatact catttcgatt gggcaatagc tttaaaaaat cagaatctga
      301 aatccaataa tccccagaag atatttcttt aattaaagat ttttgatacg ataaaggagc
      361 gaccgttacc ttgccataaa aagaagataa actagaatca aaagcttctc ggcgggggtt
      421 cccaaggatt atcgtttcag tcgccttcaa aggaatcgtc ggcgaatcca cagaaagaat
      481 tggtatttct aaaaaattta atttttccac tagttttgca aacttcgggc gtaattctcc
      541 tacggcacca attcccaata aagcatctac cactaagcaa cctgaagaag aatccatctc
      601 tagcgaatct acatcttgaa tatgggagaa aaaacctcca gcctgaataa aatcatgata
      661 agcaaggaac gcttcatttt tgaatcgtat cggatccact aagccataga ctctataagg
      721 cactttattt tttagtaata aacgagcggc aacaaaacca tcgccaccgt tattacctcc
      781 gccagtaaag atgagtactg gcttttgtgc taccgaagga tgattcgttc taaaaagctg
      841 aatcttttca taaatagcct cacccgcttc ttccattaaa gtataccctt ttcttatgga
      901 gtcgcctttt tccgatcgat ctaattcaat cgcttgcgaa gcactgatta atggaagacg
      961 tatttcatct aatttaactt tttgctgatt catatctata aatttaatga aatcaagtat
     1021 catctagcta gttattaatt caaaataaaa ctttattgta cttactccat taaagactaa
     1081 gcttctttat acaacctatg aactgacagt tgaaggaaac aattgatcca caaacgagaa
     1141 ttagataaat caaaccatga atccgaaatg taaccaacaa atggatttgt ttttatagac
     1201 aaaacggatc caccctttat tcattttttc aaacaaaaaa acacattacc gaagtaatgt
     1261 gttaaaagtt tcataaatct attttgaaat aaagatttac tttagcgtca acatggaatc
     1321 atcccatgac tcatgctttt caaagccaag aagattagct gttgtagcag ctagattact
     1381 taagccccat gaaccactct ttagtgcgag tttaccacca gagacattat catataagat
     1441 acatggcact ggatttaagg tatgagctgt tttcgatttg aaagagccat ccttattttg
     1501 tttaggcaga tgagtctttt tgtctagttc atacatttcg tctgcattac catgatctgc
     1561 tgtaatcaaa gcgataccac ctaaggcatc aatcacagga agtaaacgcg ctaatgcaag
     1621 atccacagct tcaatcgcca tggtagcagc acggaaacta cctgtatgac cgaccatatc
     1681 accattaggg taattgcaac gtaatgtttg atatttgcca gacttcaatg cttcaatcat
     1741 ggcatccgtc acttctgcag acttcatcca aggacgttgt tcaaaaggaa tcacatctga
     1801 aggcacttct aaataagttt caccatcaaa tttgctgcta cgatttccat tccagaaata
     1861 agttacgtga ccaaattttt gagtttcaga acaagcaaat tgtttaatgc ctgatttact
     1921 aaaccattct ccgcttgtat ttttaattgc tggaggagga actaagaaac gatttggaag
     1981 tttaaggtcg ccatcatact gaagcatacc agcataaacg accttaggga atctaacgcg
     2041 atcaaattct gtaaatgatt cttcttcaaa tgctcttgta atttcaatgg cgcggtctcc
     2101 gcggaaatta aagaagacta cagcatcgcc atcttgaatc gttccaactg gagatccatt
     2161 tttaccaatg acaaaaggtg gtaaatcctg atcaatggct tttgtttcac cacgaagtgt
     2221 ttcaatcgct tctttagcac tatcgaataa acggccttca ccaagaacat gagtcttcca
     2281 acccttttct accattttcc agttagcccc atagcgatcc atggtgattt gcatacgacc
     2341 accaccgctt gcaatgcaag catcaaaatc aacggaacaa aggtctgcta agaaagcttc
     2401 aaatggttcc acatagtcta aagcagatgt ttcaggaaca tcacgaccat cgagaaggat
     2461 atgaacacga actcgcttca ctccatcttt tttt
//
LOCUS       DAOOBB010000239         2475 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_46805_length_2475_cov_4.532645, whole genome shotgun sequence.
ACCESSION   DAOOBB010000239 DAOOBB010000000
VERSION     DAOOBB010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46805_length_2475_cov_4.532645"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..1579
                     /gene="gatB"
                     /locus_tag="PLT31_08770"
     CDS             125..1579
                     /gene="gatB"
                     /locus_tag="PLT31_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014544977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HPW95262.1"
                     /translation="MSKYFPVIGLEIHCQLATRTKMFCSCEVEVNTSPNKHVCPVCLG
                     MPGAMPVPNKKAVEYAIRLGLALNCEIDLNAMWTRKNYFYPDLPKGYQITQTGGLPVY
                     DHPICKNGWLEVTLDDGSVKRVGITRIHMEEDAGKLVHDLSPEGSHFDANRCGTPLCE
                     IVTEPDIRSPQEAVLVLQKIKQILEYTEVSNANMENGNMRCDGNISLRASEDAPFGTR
                     AEIKNLNSFTNLEKALEVEILLQTLTLDANKEVEQCTKRFDVNNNKTIVIRSKEDAHD
                     YKYFPEPDMVRLVTDPKFVESIKNSLPELPDARRERFVNDYQITAYDAQVLTMERDVS
                     VWFDTAAKQCKNPKILANWVISELLREVKDLENGLSDVKIMPEHLAALVNLIEDNTIS
                     GKIAKQVFADMFVTGKAPDVIVKEKGLVQITDTSAIESIVSEVVQANQGQFDEFKAGK
                     VALKGFFVGQVMKKSGGKANPGLVNQILDKMAAQ"
     gene            1590..1910
                     /locus_tag="PLT31_08775"
     CDS             1590..1910
                     /locus_tag="PLT31_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014544978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95263.1"
                     /translation="MRNLLFTLFLALLFLISTPVESRAQVLDAYDSSYISTLNMTQEE
                     IEAVYGKDSSKKDSITLQQMNDENPSYIKREYDHKQQVIVGSVIMLCVALAMVFMNNY
                     NPRR"
     gene            complement(2250..>2475)
                     /locus_tag="PLT31_08780"
     CDS             complement(2250..>2475)
                     /locus_tag="PLT31_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HPW95264.1"
                     /translation="TRIEEGIKKGIEQGLQQGLEQGLQQGIEQGIQKGVEQGALNAKR
                     EMAKVLKEQGIPMETIAKASGLSEDEIKLL"
BASE COUNT          766 a          444 c          496 g          769 t
ORIGIN      
        1 cctgataaag caaataagag ataaataatg tattgcattg tagaaaaata gcttttgaat
       61 gaatagttgc tctaaaacta gattcttttt taatatattt tgcaagtaat ttacggagtt
      121 atgcatgtct aagtattttc cagtgattgg tttagaaatt cattgtcaat tagcaacacg
      181 aaccaaaatg ttttgttcct gtgaagtcga agtgaacacg tctcctaata agcacgtttg
      241 ccctgtttgt ttaggaatgc caggtgccat gcctgttccc aacaaaaaag cggtggaata
      301 tgcgattcgt ttgggtcttg ccttaaattg tgaaatagat ctcaatgcga tgtggactcg
      361 taaaaattat ttttaccctg atttacctaa gggctatcaa atcactcaaa cgggtggttt
      421 acctgtttat gatcatccga tctgtaaaaa tggttggttg gaagtgactt tggatgatgg
      481 ttcagtcaag cgcgtgggga tcactcgtat tcacatggaa gaagatgctg gtaaattagt
      541 gcacgactta tcgccagaag gtagccattt tgatgccaac cgctgtggaa cccctctctg
      601 tgaaatcgtg acagaaccag atatccgtag tcctcaagaa gcggtgctcg ttttacaaaa
      661 aattaagcaa attttagaat acaccgaagt ttctaatgcc aatatggaaa acggaaacat
      721 gcgttgtgat ggaaatattt ctcttcgtgc aagtgaagac gcgccttttg gaactcgtgc
      781 agagattaaa aacttaaata gctttaccaa cttagaaaaa gcattagaag tagaaatttt
      841 actacaaacg ctcactcttg acgctaacaa agaagtagaa caatgcacca aacgttttga
      901 tgtgaacaat aataagacga ttgtgattcg ttccaaagaa gatgcacacg actataaata
      961 cttcccagaa ccagacatgg ttcgtttagt cacagatcca aaatttgtag aatccatcaa
     1021 aaattcatta ccagaattac cagatgctcg tcgggaacgc tttgtcaatg attatcaaat
     1081 taccgcttat gatgctcaag tgctcactat ggaacgagat gtaagtgttt ggtttgatac
     1141 tgctgcaaaa caatgtaaaa atccaaaaat tctagccaac tgggtgattt cagaattgtt
     1201 gcgtgaagta aaagatcttg aaaatggtct tagcgatgta aaaattatgc cagaacactt
     1261 ggctgctctt gtcaatctca ttgaagataa taccattagc ggaaaaattg caaaacaagt
     1321 gtttgctgac atgtttgtta caggtaaagc accagatgtg attgttaaag aaaagggcct
     1381 agttcaaatt accgatacta gtgctattga atccattgtt tcagaagtcg ttcaagcaaa
     1441 tcaaggtcag tttgatgaat tcaaagcagg gaaggtggcc ttaaagggtt tctttgtcgg
     1501 ccaagtcatg aaaaaatctg gcggaaaagc aaatccaggt ttagtcaacc aaatcctaga
     1561 taaaatggcc gctcagtaag taggattttg tgagaaacct actcttcact cttttccttg
     1621 cacttttatt cttgatttct acccctgtag aatcaagggc tcaagtttta gatgcttatg
     1681 attctagtta catcagtact ctaaacatga cacaagaaga gattgaagca gtctatggca
     1741 aagattcctc aaaaaaggat tcaatcacac tacagcaaat gaatgatgaa aacccaagct
     1801 atatcaaaag agagtatgac cataaacagc aggtgatcgt tgggagcgta attatgttgt
     1861 gcgtggcgct ggctatggtt tttatgaata attacaaccc aagacggtga tttgtttttt
     1921 cattaatcat attattgatt gaaatggtat tttcattacg taatctaaat aatcattgtt
     1981 gacggaaatg gtattacctc ctcactctgt tgtgtgccac acaagagagt tcgtcggcaa
     2041 catccatttc cgtctgctat gaaactattg ttatttaatt taatgatgtt gttttttcat
     2101 ttgaatctgc gctattgaat ttacaattca aaatacgctt gtctctgcat gaaaaaaact
     2161 gggttgagtt agatgagcgt tttattgtct cggcatgaaa aaactaggtt gggttagata
     2221 agcatttcat tgtctcggta tgaaaaaagc tacaacaatt tgatttcgtc ttcggagagg
     2281 cctgaggctt ttgcaatggt ttccattgga atgccttgct cttttaagac tttcgccatt
     2341 tcacgttttg cgttaagcgc accttgctcg acacctttct ggataccttg ttcaatacct
     2401 tgttgaaggc cttgttcaag accttgttga aggccttgtt caataccctt ttttatccct
     2461 tcttcaatcc gggtt
//
LOCUS       DAOOBB010000240         2447 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_47565_length_2447_cov_4.054348, whole genome shotgun sequence.
ACCESSION   DAOOBB010000240 DAOOBB010000000
VERSION     DAOOBB010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47565_length_2447_cov_4.054348"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(246..761)
                     /locus_tag="PLT31_08785"
     CDS             complement(246..761)
                     /locus_tag="PLT31_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95265.1"
                     /translation="MKSIKILFLSLMICSVSAFAVKGDSFLDVAPAISFVGQSNLKQP
                     NYGLSLGYMFGVNDRTDLAIYGDVYIGQSDFIGVEKYLSTSVGFRSYFTPYYGDIRPM
                     FGLGMGMSYQKQGKLIDNALFKASAHARILYDASDVIKVFTEFEPSIYLGTDPHMDFA
                     IHIGAQLRLGS"
     gene            complement(765..1085)
                     /locus_tag="PLT31_08790"
     CDS             complement(765..1085)
                     /locus_tag="PLT31_08790"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HPW95266.1"
                     /translation="MKRINLFIMALLLCSLAACTAPRTTVTTDGQSGSILFKATPGSA
                     DVYLDENPVGKAREFNGTASVMKVSPGTHTIRVSAPGYKDYVTKVYISDSQEVIDVNL
                     ESME"
     gene            complement(1085..2020)
                     /locus_tag="PLT31_08795"
     CDS             complement(1085..2020)
                     /locus_tag="PLT31_08795"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HPW95267.1"
                     /translation="MINGKIILSSFLFSLLIGCAGSVQERNVEAPPAMNPDAVVNYNG
                     LKHRLAISKFEDKTGYGSNLFGALDDLGSQASDILASHLIKTGEFVIVERENLASLEK
                     ENNLQGKSSKFAGVSALIFGAVTEFGTKTEWEDHGVSKTKVQTAHAKVTIRLVNPETG
                     VAFYSEFGEANAKNETTQTLGFGGKATYDATLTDKALNGAITKLISNVLLNLRALPWK
                     TAVLDIQDNQIIIGAGKGSGLKVGDVLEVQRPGKTVENPTTGAMMTLPGTTIAKIQVS
                     SMFGKSIMDEGAVCSVISSSGAIQKYDDVIMEGQQ"
     gene            complement(2007..>2447)
                     /locus_tag="PLT31_08800"
     CDS             complement(2007..>2447)
                     /locus_tag="PLT31_08800"
                     /inference="COORDINATES: protein motif:HMM:NF017459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 family lipoprotein"
                     /protein_id="HPW95268.1"
                     /translation="PILYDILSNRGYYCVSPELARMVFNANKLNEAGRIHEIPPNKFR
                     EIFAADAILKTRVTDWSVSYVVISSSITVTLEMELFDSKTGELLWSMTRTLQQSSGSA
                     NAGLIGSLINAAISTATFEYEPIAEDNTAFMFKTIPEGPYYDKW"
BASE COUNT          793 a          453 c          451 g          750 t
ORIGIN      
        1 gttagagtca aaactgatct tggttggatt caaaacaaaa tttataagaa gtaatatttc
       61 tttgttatga agcaggagta ttaatcaaaa aaaagcccgt gtattacacg ggctttttta
      121 atagataaat cgaatcaaag aatacaagca taagttcaaa tcaaaaatta taagcataag
      181 ttcaaatcaa aaattacgag cctaagatcc aatcaaagaa tatgagctta agatgcaatc
      241 aaagattacg agcctaagcg aagttgagca cctatgtgaa tcgcaaagtc catatgagga
      301 tctgttccta aataaataga gggttcaaat tctgtgaata ctttgatgac atcagaggca
      361 tcgtaaagaa tacgagcatg agctgaggcc ttgaataaag cgttgtcaat aagcttaccc
      421 tgtttttgat aagacatacc catccctaat ccaaacatag ggcggatatc gccataatag
      481 ggagtgaaat aagaacgaaa gccaacgctt gtggaaagat atttttctac tccgataaaa
      541 tcactttggc caatgtaaac atcaccataa atggctaagt ctgttctatc gtttacgcca
      601 aacatatagc ctaaagataa accgtagttt ggttgcttta agttgctttg tccaacaaaa
      661 ctaatcgcag gagcaacatc caaaaaagaa tctcctttta ctgcaaaagc agaaacagag
      721 catatcatta aggaaagaaa aagaattttt atgcttttca ttttttactc catggattca
      781 agattaacat caatgacttc ttgtgaatcg ctaatatata ctttcgttac atagtcttta
      841 tatccaggag cactgacgcg aatagtatga gtgcctggag aaactttcat aacactagca
      901 gtcccgttga attcacgtgc tttacctact ggattttcat cgagataaac atcagcactg
      961 cctggagttg ctttgaataa aatgcttcca gattgaccat ctgtagtgac tgttgttcta
     1021 ggcgctgtac aagcggccaa agaacataat aataaggcca ttataaatag attaatgcgt
     1081 ttcattattg ttgaccctcc atgatgacat catcatattt ttgaattgca ccactagatg
     1141 aaataactga acaaacagct ccttcatcca ttatggattt tccaaacata gacgagactt
     1201 gaatttttgc aattgttgtt cctggtaaag tcatcatagc accagtagtt ggattctcta
     1261 ctgtttttcc tggacgttgt acttcgagaa cgtctcctac ttttaagcca gagcccttac
     1321 ctgctccaat gataatttga ttgtcttgaa tatctaaaac agcagttttc caaggtagag
     1381 ctctaaggtt taatagaaca ttactaatta gtttagtaat agcaccattt aatgctttgt
     1441 ctgtgagcgt cgcgtcataa gttgcttttc caccaaagcc aagagtttgt gtagtttcgt
     1501 tttttgcatt ggcttctcca aattcggagt agaaggcaac acctgtttct ggattgacta
     1561 aacgaatagt aaccttggca tgagcggttt gcactttagt ttttgaaacc ccatggtctt
     1621 cccattctgt tttagttcca aattctgtta cggcaccgaa aataagtgcg gaaactcctg
     1681 caaactttga agacttgcct tgaagattat tttctttctc taaagaagca aggttttctc
     1741 tttctactat tacaaactca ccagttttaa ttaagtgaga agcgagaatg tctgatgctt
     1801 gtgatccgag atcatctagg gctccaaata aattagatcc atagcccgtt ttatcttcaa
     1861 atttagaaat tgctaaacga tgttttaagc cattgtagtt tactacggca tcagggttca
     1921 ttgcgggagg ggcttctaca tttctttctt gaacggagcc cgcgcaaccg atcagtaatg
     1981 aaaaaagaaa tgaagaaagg atgattttac catttatcat aataaggtcc ttctgggata
     2041 gttttaaaca tgaaagctgt gttgtcttct gcaataggtt cgtattcgaa agtggctgtg
     2101 gaaatagcgg cattaattag actaccaata agtcctgcgt tagcagagcc tgatgattgt
     2161 tgtaatgtgc gcgtcattga ccacaggagt tcacctgttt tagaatcaaa taattccatt
     2221 tcaagagtaa ctgtaatgga agatgaaata acaacgtagc taacagacca atccgtaact
     2281 ctagttttta aaatggcatc ggcagcaaaa atttccctaa atttgttagg aggaatctca
     2341 tgaatacgcc ctgcttcatt aagtttattt gcgttaaaaa ccattctagc taattctgga
     2401 ctaacgcaat aatagcctcg attagataaa atatcatata aaatagg
//
LOCUS       DAOOBB010000241         2422 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_48190_length_2422_cov_6.887621, whole genome shotgun sequence.
ACCESSION   DAOOBB010000241 DAOOBB010000000
VERSION     DAOOBB010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48190_length_2422_cov_6.887621"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..191)
                     /locus_tag="PLT31_08805"
     CDS             complement(45..191)
                     /locus_tag="PLT31_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95269.1"
                     /translation="MVLVLFGCLLSDDPANNEKDDINIEKEAKDNIEGDGNEGQDNDE
                     EVPY"
     gene            590..952
                     /locus_tag="PLT31_08810"
     CDS             590..952
                     /locus_tag="PLT31_08810"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPW95270.1"
                     /translation="MLTNQLVPSTFFGLKDFFNNFEDSKTSFPAAAIYEAENGYTLEV
                     ELPGVKKEDVSIEMERNVLTIKGSRKRGETETTYERSFRVADQIDSDNVTAVMEDGVL
                     RVSLNKKKESEAKKIVIQ"
     gene            complement(999..2300)
                     /locus_tag="PLT31_08815"
     CDS             complement(999..2300)
                     /locus_tag="PLT31_08815"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPW95271.1"
                     /translation="MILLARTIIFLFLLGGFSVFASSTAWTLEDCMAQAKKASLKLES
                     SKLNEESASISLKKAKSSRYPDLSANINNSLYDSPFNSGAQDHYRLSLGISSSMNLWD
                     GGATNLNIQSNTLKKQSSSFETEQIWQNIQESVLNAYISLLAAQENIQVARSALLLAE
                     EELKRIQYLYDVGSATQKDFVLAKSELAQKKSAALVAEQTLENQKTTLRQLLEIPRED
                     SFEIVSPKWDFSSPMDAPALLPLNELLVEVRQKNPGLQADSINVLVSEKEKLIASKTS
                     SIKVSLGASASTGLTAWESNAYGNQMKNGYTHSISLGVSIPIIDNGVETSNVLQAQIG
                     VTQAQLNKRETEKNLENVIETLYLNAKSADLQWEAALLQKEAEEEAYRVVEEQRIMGM
                     ISYTDFLEQKNKLESAQLNLNSAKYNSLLMRKRIDLYRAAF"
BASE COUNT          748 a          473 c          374 g          827 t
ORIGIN      
        1 tgcttatgta ttcggcagga tccactttat gtaacttgca tgtttcaata agggacttcc
       61 tcatcattat cttgaccttc attaccatcc ccttctatat tatccttcgc ttctttctca
      121 atatttatat cgtctttttc attatttgca ggatcatcgg ataataaaca gccaaataat
      181 acgagcacca ttattgataa tagcactaga aatatttttt tcacgagata atccccttta
      241 tacctaaaat tattttttca caccaacatc gatgttactt tctataatat aaaacaaatg
      301 attatctatt ttgaatcata acaaaacggc gtgattgttg attctcaatt tctgcaataa
      361 ttaattgtag tcactttatt tctgatattg attatctagt ctatgtttta aaacaaacca
      421 aatacgcgtt taattcataa acaaaaatgt ttctttttga atagctcctt ttaaaaaagg
      481 ggcttttttc ttttttttga ttaaacctac ccatttcaaa gtattggcat cacatttgct
      541 ttatacctat cagaaacaag aagtttatac agtcataagg agatacacca tgttaacgaa
      601 tcaacttgta ccaagcactt tttttggatt aaaagatttc tttaataact ttgaggattc
      661 aaaaacatca tttcctgccg ccgctattta cgaagcagaa aacggttata ctttagaagt
      721 agagcttcct ggagttaaaa aagaagacgt ctccatcgaa atggaaagga atgttcttac
      781 cattaaaggc tcccgcaaac gtggagaaac agaaacaacc tacgaacgtt ccttccgcgt
      841 ggcagatcaa atcgattctg ataatgtcac tgcggtaatg gaagacggtg tacttcgtgt
      901 ctctttaaat aagaagaaag agtccgaagc gaaaaaaatt gttatccaat aaaagacaca
      961 aagccaaatg aagtccttcg aaagaagggc ttcatttttt aaaatgccgc tcgatacaaa
     1021 tcaattcgct ttctcatcaa taggctgtta tactttgcac tatttaaatt gagttgcgct
     1081 gattccagtt tgtttttttg ttctaaaaaa tcggtataag aaatcatacc cattattctt
     1141 tgctcttcaa caacgcggta tgcttcttct tcagcctctt tttgcagaag agctgcttcc
     1201 cattgaagat ccgctgactt tgcatttaag tataaagtct ctattacatt ttccagattc
     1261 ttttctgttt ctcttttatt taattgagct tgtgtcactc ctatttgagc ctgcaaaaca
     1321 ttcgacgttt ctacgccatt atctataata ggaatactga cacctaatga gatactatgt
     1381 gtatacccat ttttcatttg attgccataa gcattagatt cccatgccgt taaacctgtc
     1441 gacgccgaag cgccaagtga aacttttata gaactcgttt tagaggctat caatttttct
     1501 ttttctgaaa ctaaaacatt aatactatcc gcttgtaacc ctggattttt ttgcctcact
     1561 tccactaaca attcatttaa aggcaataaa gctggcgcat ccataggaga agaaaaatcc
     1621 cacttaggcg atacaatttc aaaagaatct tctctcggaa tttctaataa ttgccttaaa
     1681 gtagtctttt gattttcaag cgtttgttct gcaacaagag ctgcactctt tttctgtgcg
     1741 agttccgatt tagctaaaac aaaatctttt tgagtcgcgc tcccgacatc atataaatac
     1801 tgaattctct ttaattcttc ttctgctaaa aggagtgccg accgagccac ttgtatgttt
     1861 tcttgagccg cgagcagaga aatataagca ttaagaacag attcttgtat attctgccat
     1921 atttgctctg tttcaaaaga agaactttgt ttttttaatg tattactttg aatattcaaa
     1981 tttgtagcgc caccatccca caaattcatt gaactagaaa tccctaaact caaacggtaa
     2041 tgatcttgag cgcctgagtt aaatgggcta tcgtaaagag aattattaat gttcgctgat
     2101 aaatcaggat atcgactact ttttgctttc tttagagaaa tagaagcgga ttcttcattt
     2161 agcttagaag attctaattt caagctcgct ttttttgctt gagccataca atcttcaaga
     2221 gtccatgccg tcgaagaggc aaaaactgaa aaaccaccca gtagaaacag gaaaataatg
     2281 gtacgcgcta aaagaatcat acattttaga tgattcaaat acatgaatgg ttccaatcag
     2341 gattggtttt ttgtatctgc atcatccttt ttaagacttg tatcgacgtt cgccgcttca
     2401 gaattagaaa gatcttcttt gt
//
LOCUS       DAOOBB010000242         2420 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_48250_length_2420_cov_3.452431, whole genome shotgun sequence.
ACCESSION   DAOOBB010000242 DAOOBB010000000
VERSION     DAOOBB010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48250_length_2420_cov_3.452431"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PLT31_08820"
     CDS             complement(<1..182)
                     /locus_tag="PLT31_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPW95272.1"
                     /translation="MKLILPVAGKGERLKPYTLILPKCLLPVAGKPILNWIIESTQQL
                     PITETLFITGYKSECI"
     gene            562..2127
                     /locus_tag="PLT31_08825"
     CDS             562..2127
                     /locus_tag="PLT31_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HPW95273.1"
                     /translation="MSSSNVKADRVPFFYDVTLRDGNQALAKPWNNEEKEKVYLQLLK
                     LGVQGVEVGFAAASDMDFESCKGIAQLTASMASAGDVSAQKVVVASLARANKVDIERA
                     WEAVQYAPKPRIHTFIATSKLSMEHVLKMSPEQVRARAVESVALAKSLVGDRGDVQFS
                     AEHFGDCADNMDFVIDLLKAVVEAGATTINLPNTVERYRPMLYVEQVRQVVDALPKEV
                     IVAVHCHNDLGMATAATVESFFVGATQLEVALNGLGERCGNTNAYEVAVALFNSGVPV
                     ALNMDRIYETALLVSQWSNVNIYSRAPLIGAEAIVHRSGIHQDGASKTKNMKKGAYRP
                     IDYALIGRHENDSISFTSQSGRTAVYEIITKFGYHLSLEEAAELQPILKELSEKEGEL
                     SVDRVLDVFRRERCNVNGRLIFNNVEVLPGENRFVFHFKKDGEPVVKSETAEGPIEAC
                     LMLMRDISMPVELVKYRQLVVPESDKLWAGRGLSRIILKAGDKVVEGRGVSSDTLKAN
                     MRALFSGVNLLYK"
BASE COUNT          628 a          387 c          619 g          786 t
ORIGIN      
        1 tctatgcatt ctgatttgta gcctgtaata aagagggtct ctgtaatggg gagttgctgc
       61 gtgctttcga taatccaatt gagtatgggt ttaccagcaa caggtaataa gcatttaggc
      121 aaaattaaag tatagggctt taatcgttca cctttgcctg caacgggtaa aatcaatttc
      181 atgcttgttt ggcctctttt ttagttgtaa tatttctgta acattctgaa agatagtaat
      241 gtttttttat tattatttaa cttttatggc tttttttaat gttttgcctt atattaaaag
      301 gtttttaata cttaagtatt acgaactaat atgcaaaaaa cggcattttt gtacggtttt
      361 ataggaaagg ggtgtgcaaa acccccaatt ttgttatatt attttccatg attcaccgta
      421 attttttatt agccctcctt ctaagtcgct tttgaagcgg ggtttttacg gtataataaa
      481 aaacggcctg cttcgatttc gaggtgggct ttgttttttt tcttagcctc ttcgctgttg
      541 gagtttggca aggagataaa aatgagtagt agtaatgtca aagccgatcg agttccattc
      601 ttttatgatg tgactcttcg tgatggtaac caagcgttgg ccaagccttg gaacaatgaa
      661 gaaaaagaaa aggtgtattt gcagttgtta aagcttggtg tgcaaggtgt tgaagtaggc
      721 tttgcagcgg cttctgatat ggattttgaa tcttgtaaag ggattgcgca attgactgcc
      781 tctatggcta gtgctggcga tgtttcggct caaaaggtgg tggtggctag tttagctaga
      841 gccaataagg tagatattga gcgcgcatgg gaggcggtgc agtatgcgcc taagcctcga
      901 atccatacgt ttattgcaac aagtaagctt tcgatggagc atgtgttaaa gatgtctcca
      961 gagcaagtgc gtgctcgtgc cgtagagagt gtggctttag caaaatcgtt ggttggtgat
     1021 cgtggcgatg tgcagtttag tgcagagcat tttggcgatt gtgctgataa tatggatttt
     1081 gtaatcgact tgttgaaggc cgtagtagaa gctggtgcga ctacgattaa tttgccaaat
     1141 acggtggagc gttaccgccc tatgctttat gtggagcagg ttaggcaggt agtagacgct
     1201 ttgcctaaag aagtgattgt ggctgtgcat tgtcataacg atttgggtat ggctacggcg
     1261 gctacggtcg agagtttctt tgtgggggca actcagttag aggtggcttt gaatgggctg
     1321 ggcgagcgtt gcggtaatac gaatgcgtat gaggtggctg tcgctttgtt taattctggc
     1381 gtgcctgtgg ctttgaatat ggatcgtatt tatgagacgg ctttgcttgt gagccagtgg
     1441 tctaatgtga atatttattc gagagctcct ttgattgggg cagaggcgat tgtgcatcga
     1501 agcggtattc atcaagatgg ggcttctaaa acaaagaaca tgaaaaaggg tgcgtatcgc
     1561 ccgattgatt atgctttgat tgggcgtcat gaaaatgatt cgatttcgtt tacaagccaa
     1621 agcggtagaa cggcggtcta tgaaattatc accaaatttg ggtatcattt gtctttagaa
     1681 gaagcggccg aattgcagcc tattttaaag gaattgagcg aaaaagaagg cgagttgtct
     1741 gtggatcgcg tgctcgatgt ttttaggcgt gaacgttgta atgtaaacgg gcgcttgatt
     1801 tttaataatg tcgaggtgtt gcctggcgag aatcgctttg tgttccactt taaaaaagat
     1861 ggcgaaccag tggtgaagtc agagactgca gaagggccaa ttgaagcgtg tttaatgctg
     1921 atgcgtgata tctctatgcc tgtggagctt gtgaagtatc gtcagttggt ggtccctgaa
     1981 tccgataagc tttgggctgg tcgcgggctt tcaaggatca tcttaaaagc aggcgataaa
     2041 gtagttgaag ggcgtggcgt ttcaagcgat acgctcaaag ccaatatgcg agcgcttttt
     2101 tctggtgtaa acttgttata caagtaacta taaaaccctc ttgtaactac aagagggttt
     2161 tcatttgaaa atattttttt aatggaaaag tctgtttgat tttttgtcaa atagactttt
     2221 ttatttaatg aaaaaacaaa tgtaaataaa ataattgtgt gctacacaac aagaacactt
     2281 tgccctttgg ggacgattaa acacttgctt cttcataaaa aattacaaag acttgacttc
     2341 ttcttcagaa aggcctgatg cttttacaat cgcacctatc gggaaacctt gttctttaag
     2401 tgatttcgcc atctcacgag
//
LOCUS       DAOOBB010000243         2385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_49125_length_2385_cov_4.019313, whole genome shotgun sequence.
ACCESSION   DAOOBB010000243 DAOOBB010000000
VERSION     DAOOBB010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49125_length_2385_cov_4.019313"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..654)
                     /locus_tag="PLT31_08830"
     CDS             complement(142..654)
                     /locus_tag="PLT31_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPW95274.1"
                     /translation="MWRHLFAILLLLTMTSFAEDGLRIAHVDSKLIFDGYKGTRKAQE
                     EFDRQVAKWEQQANLLQKELASIKERLDKQSLMLSDEKKRELEADYAKKDTELKDFIS
                     KVYGRTGELISENEKVSAPIIQLIRKAVNEIALQEGYDMVVDRATGAVLFWKNENDLT
                     QKVLDYLNSR"
     gene            complement(666..>2385)
                     /locus_tag="PLT31_08835"
     CDS             complement(666..>2385)
                     /locus_tag="PLT31_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPW95275.1"
                     /translation="EKWISALPQKSWKNAKIDSLLKLKDRSVFDDKLLVRYTIEEQFS
                     SLMPYDLIKTSNAIKISVAMNEGRRYYMGGVHFSGNQVLPEALLKRIVRLDSGAVFDY
                     YAYENFKKGFMDAYREDGYLFARFDESRTFESDSIVNLSFHFTEGLPAQVRKVFIRGN
                     TKTKDKVIRREVRLFPGDTYRQSALERSFREIMQLNYFDAVLPDIKVAGEQEVDLEFN
                     VSEREAGTGQFSLGLAYSQSDGLTFTTSVAIPNCCMGDGLSTAVNAEYGQDKKGASIS
                     FSDPWFLDKPITFGSSLSYTWWEGGTDPDITRYGGSVYVGKRLKWPDDYFYGQIGYSW
                     LMNDQGDNIDDSYVLYSGIESAINLRLIRDDKNLPQFPTDGSRYELGLQIANPIIGSD
                     FDFVKTDVTVKWWFPLFRDKLTLQLTNEYGIIAGDPLQYRTLYSMGGVLGYEGMMRGY
                     SAGSIGYRRLGRSYQYFGAELQLGIVPNTFYLLPFFFDAGNVFGDRYDPDVKVSKKQK
                     NPLREWDPSSLKKDIGFGFRVVVPMLGIIGFDFAWPLDPGETYGGLQQTGVGSMEFNF
                     VIGQGF"
BASE COUNT          775 a          519 c          375 g          716 t
ORIGIN      
        1 ctccatttat tcgaaaacaa tgtgatgcga ttgtccgaat ccctatgatg ccagatgccc
       61 attcctacaa tgcgtctgta gctctttctc ttgggctata cgaaatttca agaacaagaa
      121 ttaaataaca tgaagaaatg attaacgaga attcaaatag tctaaaactt tttgagttaa
      181 atcgttttca tttttccaga aaagaacagc gcctgtcgca cgatccacaa ccatgtcata
      241 gccctcttga agagctattt cattgactgc ttttcgtatg agttgaataa taggggcact
      301 gactttttca ttttcagaaa tcaattcacc tgtacggcca taaactttag aaataaagtc
      361 tttaagctca gtatcttttt ttgcataatc ggcttcaagc tctcgttttt tctcatcgga
      421 gagcattaaa gattgcttat ctaagcgttc tttaatagat gctaattctt tttgcaaaag
      481 gtttgcttgc tgttcccatt tggccacctg acgatcaaac tcttcttgtg cttttcgagt
      541 tcctttatag ccatcaaaaa tcaattttga atccacatga gcaatccgta agccatcttc
      601 agcaaaagac gtcatggtta atagtaataa aattgcaaat aaatgacgcc acataagtac
      661 tccttttaga agccctgtcc tatgacaaag ttaaattcca tcgagccaac gcctgtttgt
      721 tggagacctc cgtatgtttc acctggatct agaggccaag cgaaatcaaa cccaataata
      781 ccaagcatag gaactacgac gcggaagcca aaaccaatgt cttttttcaa agaagaagga
      841 tcccattcac gtaacggatt tttttgtttt ttggaaactt ttacatctgg atcatatcga
      901 tcgccaaaaa cattacccgc atcaaagaag aatggtaata aatagaacgt atttggaaca
      961 atgcccaatt gcaattcagc accaaaatac tgataactgc gccctaatct tctatacccg
     1021 atagaaccag cactataacc acgcatcatt ccttcataac ctaacacgcc acccatagaa
     1081 tatagtgtac gatattgtaa tggatctcca gcaataatac catattcatt agttaattga
     1141 agagtcaact tatctctaaa taatgggaac caccatttaa cagtcacatc cgttttaaca
     1201 aaatcaaaat cactaccaat aattggatta gctatctgta agccaagttc ataacgtgaa
     1261 ccatctgtag ggaactgagg tagattttta tcgtctcgaa tcaatcgtaa attaattgca
     1321 gattcgatac cagaatacaa cacatagcta tcatctatgt tgtcgccctg atcattcata
     1381 agccagctat atccaatttg tccatagaag taatcgtctg gccatttcaa acgcttaccc
     1441 acataaacac taccaccgta tcttgtgata tctggatctg ttcctccttc ccaccatgta
     1501 taacttaagc ttgaaccaaa agtaattggt ttatctaaga accatggatc agagaagctg
     1561 atggaagcgc cttttttatc ttgaccatat tcagcattta cagcagtact taagccatca
     1621 cccatacaac agttagggat agcaacactc gttgtaaaag taaggccatc gctttggcta
     1681 tatgcaagtc caagactaaa ttgacctgtt ccagcttctc tttcagaaac gttaaattca
     1741 agatctactt cctgttcacc agcgacttta atgtctggaa gaactgcatc aaaataattc
     1801 aattgcatga tttctctaaa gctacgttct agagccgatt gacgataagt atctcctggg
     1861 aacaaacgta cttcacgacg aatcacttta tcttttgttt tggtattacc gcgaataaag
     1921 actttgcgta cttgtgcagg aagcccttca gtaaaatgga aactcagatt gacaatcgaa
     1981 tccgattcaa atgttctaga ttcatcaaaa cgagcaaata aatagccatc ttcacggtaa
     2041 gcatccataa accctttttt gaagttttca taggcataat aatcaaaaac agctccgcta
     2101 tcaagacgaa caatacgttt taaaagtgct tctggtaaaa cctgattccc tgaaaaatga
     2161 acgcctccca tgtaataacg acgaccttca ttcatcgcca cagaaatctt tatagcattc
     2221 gatgttttaa ttaaatcata aggcatcaaa gaagaaaatt gttcttcgat cgtgtaacga
     2281 accaataatt tgtcatcaaa aacagagcga tctttaagtt ttaataaaga gtcaattttt
     2341 gcatttttcc agcttttttg aggtaaagcc gaaatccact tttca
//
LOCUS       DAOOBB010000244         2359 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_49819_length_2359_cov_4.144097, whole genome shotgun sequence.
ACCESSION   DAOOBB010000244 DAOOBB010000000
VERSION     DAOOBB010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49819_length_2359_cov_4.144097"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PLT31_08840"
     CDS             <1..638
                     /locus_tag="PLT31_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HPW95276.1"
                     /translation="PSAIAKAENLPISDIPFIFEGGALEVNDDGLAMTTLDCLIGKNR
                     NDPKDLKKILSALCKKLGLRDILVLPEGLDGDHTDGHIDNLARFVETNRVVMASEEDS
                     TSPNYERLMEAKKLIEAWLKKHYGNKAKVDTLQLPPQKKMGKEILPASYMNFIFVNGG
                     LLFPSYEPKTDKKAKAYFKSVFPDRKIIAIDCRVVIQEGGALHCLSKHESA"
     gene            complement(635..1273)
                     /locus_tag="PLT31_08845"
     CDS             complement(635..1273)
                     /locus_tag="PLT31_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HPW95277.1"
                     /translation="MAKMKYYAIKTPQFEKIVTNWDECLVLIKGVKGVLYKSFKTLEE
                     AENWLKGVSEEADSSLQIYVDGSFSPDFPYSGWGLVVVENNEEQFRLGGVTPEPAKSR
                     NIDGELFAAIKALEWLKENQRVGTICHDYEGIARFAKGEWKAKSPVALEYMKKARPLL
                     GENKFKKVDAHTGHQWNDLVDQIAKDAILDAKSKPFSLEKTAEKNGQFLLEL"
     gene            1316..1885
                     /locus_tag="PLT31_08850"
     CDS             1316..1885
                     /locus_tag="PLT31_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95278.1"
                     /translation="MKPSPYFADRFSFFFFRFYSKASLFKWFLKKADAETEAFEYPSS
                     LSGSHRTIVFLPSNRELSALTLTALERFWDPDQTLLVGSVDLTNDIHKLKVKIPFISY
                     TDSDCRFGEPAFCEIEEKLVAFNAEICLYLETEPFLPRLYLVKKSAAPCRIGFYAEAE
                     FPFLNISLKPESDDSLSKIKILVQQYGDS"
     gene            1886..>2359
                     /locus_tag="PLT31_08855"
     CDS             1886..>2359
                     /locus_tag="PLT31_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPW95279.1"
                     /translation="MQSSRSTTNAKIGYADLHMHTTLSDGNLSVEELVTFCKRKGLRC
                     ISITDHDNLDSYKMALKPAEEAEIELIPGIEISAVWQGKDIHILGYYCDPTNLALNIE
                     LEDFAKQRITRVKAIVKKLNALGIDITFEKVLSYCKGKVIGRPHVAMSLVDEEYIS"
BASE COUNT          716 a          508 c          395 g          740 t
ORIGIN      
        1 tcccctccgc tattgcaaaa gcagaaaatc tcccgatctc agacatccct tttatttttg
       61 aaggcggtgc cttagaagtg aacgatgatg gccttgccat gactacctta gattgtctca
      121 tcggtaaaaa cagaaacgat cctaaagatc taaaaaagat tctctctgct ctctgtaaaa
      181 agcttgggct tcgtgatata ctcgttctcc ctgaaggctt agatggcgat cataccgatg
      241 gccatatcga taatctcgcc cgttttgtgg agacaaatcg agtcgttatg gcttcagaag
      301 aagactccac ttctccaaac tacgagcgtt taatggaagc caaaaaatta atagaagctt
      361 ggctcaaaaa acattacgga aacaaagcaa aagtggacac tcttcagctt cctcctcaaa
      421 agaaaatggg aaaagaaatt ctacccgcat catatatgaa tttcattttt gtaaatggcg
      481 ggcttttatt cccttcttat gagccaaaaa ccgataaaaa agcaaaagct tatttcaaat
      541 ctgtttttcc tgaccgtaaa attattgcca ttgactgccg agttgtgata caagaaggtg
      601 gagctctcca ctgtttatca aaacacgaat cggcttaaag ttcaagtaag aactgcccgt
      661 ttttctcagc cgttttttcc aatgaaaatg gcttcgattt tgcatctaaa atggcatctt
      721 tggcaatttg atccacaaga tcattccatt gatgccctgt atgagcgtcc acttttttga
      781 atttattttc gcctaatagg gggcgagctt ttttcatata ctctaaggca acaggacttt
      841 tagctttcca ttcgcctttg gcaaaacgcg caatgccctc ataatcatgg caaatcgtac
      901 ccacgcgttg attctctttt aaccattcta aagccttaat cgctgcaaaa agttctccat
      961 caatatttcg actttttgca ggttctggag ttactccccc caaacggaac tgttcctcat
     1021 tattttctac aacaacaagc ccccatccag aatagggaaa atcaggtgaa aaagagccat
     1081 ctacatagat ttgaaggcta gaatccgcct cttcagacac cccttttaac caattttcag
     1141 cttcttctaa agtcttaaat gatttatata aaacaccctt tacacccttg atcaaaacca
     1201 aacattcatc ccaattcgtc acaatttttt caaattgagg agtttttatt gcgtaatact
     1261 tcatttttgc catacttaaa atttatattt cttcacaacg tattttggag atcttttgaa
     1321 accttcaccc tattttgcag atcgtttttc ttttttcttt ttccgattct attcaaaagc
     1381 atcgttattt aaatggtttt taaaaaaagc agacgctgaa acagaagctt ttgaataccc
     1441 atcctcctta tctggttcac accgaaccat tgttttttta ccttctaatc gagaattatc
     1501 ggctctaacc cttactgctt tagagcgttt ttgggatcca gaccaaactt tactagtcgg
     1561 ttctgtcgat cttacaaacg atattcacaa attaaaagtc aaaatcccct ttatctctta
     1621 cacagatagt gattgtcgct ttggtgagcc tgctttttgt gaaatagagg aaaaactagt
     1681 cgcttttaat gctgaaattt gcctttatct tgaaacagaa ccctttttac caagattgta
     1741 tttagtcaaa aaatcagctg ctccttgccg aattggtttc tatgccgagg ccgaatttcc
     1801 attcctaaat attagtctaa aaccagaaag cgatgattcc ttatcgaaaa taaaaatcct
     1861 tgttcaacaa tatggggatt cttaaatgca aagtagtcgc tctacaacaa atgctaaaat
     1921 cggctatgcc gacttgcata tgcatacgac gctttcggac ggaaaccttt ctgtagagga
     1981 attggttact ttttgcaaaa ggaaaggact tcgctgtatt tccattacag atcacgataa
     2041 cttagactcc tataaaatgg ccttaaaacc agcagaagaa gccgaaatag agctcattcc
     2101 tggcattgaa atttctgctg tatggcaagg gaaagatatt catattctcg gttattattg
     2161 cgatccaacg aatttagctc taaatattga attagaagac tttgccaaac aaagaatcac
     2221 tcgcgtgaaa gcgattgtaa aaaagctaaa cgcacttggt attgatatca cttttgaaaa
     2281 agtactttct tattgtaaag ggaaagtcat agggcgtcct catgtcgcga tgtctcttgt
     2341 agacgaagaa tatatttcc
//
LOCUS       DAOOBB010000245         2340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_50350_length_2340_cov_4.076586, whole genome shotgun sequence.
ACCESSION   DAOOBB010000245 DAOOBB010000000
VERSION     DAOOBB010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50350_length_2340_cov_4.076586"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PLT31_08860"
     CDS             complement(<1..203)
                     /locus_tag="PLT31_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95280.1"
                     /translation="MSINEKIFTDKSIHSRFGRLKKALIQGIGIFIVFLYTGCLDIPP
                     TPDESIKVKAIGLWLIKDNDSDSL"
     gene            234..455
                     /gene="xseB"
                     /locus_tag="PLT31_08865"
     CDS             234..455
                     /gene="xseB"
                     /locus_tag="PLT31_08865"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HPW95281.1"
                     /translation="MSQANLEYKTAMAALEEILTRIDDSDMGIDELASEVERATILLR
                     ECRKILMDTEKKIQDTLASFDGDVDSNGD"
     gene            433..1407
                     /locus_tag="PLT31_08870"
     CDS             433..1407
                     /locus_tag="PLT31_08870"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPW95282.1"
                     /translation="MSIPMATEALTIENLTVRYPMRGGVLGRVQQFFTAVEDFSFSLE
                     TGEILAIVGESGCGKSTLAQALVGLAPWTIGKMTLLGEELKPSSRKDLTLIRRQMQMV
                     FQDPFSSLNPRQTVSEILMYPLLARGFSRIESSQKIKETLSLVGLSTEVLSRFPHAFS
                     GGQRQRIGIARSLVLSPKILICDEVTSALDVSVQAQVIQVLDEIRRQAGISLIFISHD
                     MQVVRALADNVIVMYLGQIMEKGSVSQVLNSPKHPYTKALIKSIPTLDREHPPEILEG
                     DWATHASPKTGCRFAPRCPQRMPHCTEDSIPLDSMDSVLCRCVLGRPS"
     gene            1444..>2340
                     /locus_tag="PLT31_08875"
     CDS             1444..>2340
                     /locus_tag="PLT31_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HPW95283.1"
                     /translation="MKLSIEKMVQGGVGFTRLPDGRVCFVPQALAGEEVEVAISKESK
                     DYVEARVMQIIKPSPIRIKPKCPLYEKCGGCSLQHVSLESQVLIMKDIVKETFLRIGK
                     TALPDDFPIFSTEPWHYRHRARVVKDRGTKQFGFREEQSHRIISFEECPVLTKSLNSF
                     LHLSSTQKIPTQELHCFDNGSGRVSYFYKGMKEADQRLLSDNQVLIGDRNISMDASVF
                     FQSNLSLLSTLVDRVRVHAEKGDWLIDLFSGVGFFASLLEDRFSRITTVEREPGCLRH
                     AKKNLKKAAEQVTANAESWLLEN"
BASE COUNT          694 a          406 c          483 g          757 t
ORIGIN      
        1 agggaatctg agtcattgtc tttaattaac catagaccga tggccttgac ttttatagac
       61 tcatctggcg ttggaggaat atctaagcat ccagtataca aaaaaacaat aaatatgccg
      121 atgccttgga ttaatgcttt ttttaatcgc ccaaaccgag aatgaattga tttgtcagtg
      181 aatatttttt cgttgatact cattaaagat aaatgtaact atattccatt attatgagtc
      241 aggcaaattt agaatataag acagcgatgg ccgctttaga ggaaatttta acccgtatag
      301 atgattctga tatgggaatt gatgaattag cttctgaagt cgagcgagct accattctat
      361 tacgtgaatg tcgtaaaatt ttaatggaca cagagaagaa aatacaagac acattagcct
      421 cttttgatgg ggatgtcgat tccaatggcg actgaagcct taacaattga aaatttgacg
      481 gttcgttacc ctatgcgagg tggcgtttta ggtcgtgtac aacaattttt tacagcggta
      541 gaagattttt cattttcttt agaaacaggt gaaattctcg ctattgttgg tgaatctgga
      601 tgcggaaaat ctactttagc gcaagcatta gttggtctag ctccttggac tatagggaaa
      661 atgactctct taggtgagga gttgaaacca tcttctcgaa aagatttgac tttgattcga
      721 agacaaatgc agatggtttt tcaagaccct ttttctagtt taaatccaag gcaaaccgtt
      781 tcagaaattt tgatgtatcc attactggct cgtggctttt cgcgtataga atcaagtcaa
      841 aaaattaagg aaacactttc tttagtaggc ctttctacag aagtactttc gagatttcct
      901 catgcttttt ctggaggtca gcgtcaacgt attggcattg ctcgttctct agttttatct
      961 cctaaaattc ttatttgtga tgaagtgacg agtgctcttg atgtttctgt ccaagctcaa
     1021 gtcattcaag ttttagatga aattcgtcgt caggctggaa tttctttaat ctttatttct
     1081 catgatatgc aggtcgtccg agctcttgcc gataacgtga ttgtaatgta tttgggtcag
     1141 attatggaga agggaagtgt ttcccaagtc ttaaactcac ctaaacatcc atataccaaa
     1201 gctttgatca aaagtattcc tactttagac cgagaacatc ctccagagat attagagggc
     1261 gattgggcaa cacatgcttc accaaaaacg ggttgtcgtt ttgcaccacg ttgtcctcaa
     1321 agaatgcctc attgtacaga agattcgatt cctttagatt cgatggattc tgttctttgt
     1381 aggtgtgttt tagggcgacc gtcatagatt gtttctttaa atttgattat aaggaatcgc
     1441 tttttgaaat tatcaattga aaaaatggtt caaggaggag ttggttttac tcgacttccc
     1501 gatggccgtg tctgttttgt accacaagca ttagctggag aagaagtaga agttgctatt
     1561 tcaaaagaaa gtaaagacta cgtagaagct cgtgtgatgc aaatcatcaa gcctagcccc
     1621 attcgaatta aaccgaaatg tcctttatac gaaaaatgtg gtggttgttc tttacagcac
     1681 gtttctttag aatctcaagt actgattatg aaggatattg tcaaagaaac tttcttaaga
     1741 atagggaaga cagcattacc agacgacttt cctattttct ctacagagcc ttggcattat
     1801 agacatcgag ctagggtcgt taaagataga ggcacaaaac aatttggttt ccgagaagaa
     1861 caatcacatc gtattatttc ttttgaagaa tgccccgttt taacaaaatc attaaatagt
     1921 ttcttgcatt taagttctac acaaaaaatc cctactcaag aacttcactg ctttgataac
     1981 gggagtggcc gagtctctta tttttataag ggaatgaaag aagcggatca acgattgctt
     2041 tcagataatc aagttttgat aggggatcgt aacatttcta tggatgcgtc tgtctttttt
     2101 caatcgaatc tttctttgct ctccacgtta gttgatcgtg tgcgagtcca tgcagaaaaa
     2161 ggagattggt taattgatct ttttagtgga gttggttttt ttgcaagcct tttagaagac
     2221 cgattttcaa gaatcacaac ggtggaaaga gaacctggtt gcttgcgcca tgcaaagaag
     2281 aatttaaaaa aggctgctga acaagtgact gcaaacgctg aatcatggct tttagaaaac
//
LOCUS       DAOOBB010000246         2325 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_50788_length_2325_cov_4.252863, whole genome shotgun sequence.
ACCESSION   DAOOBB010000246 DAOOBB010000000
VERSION     DAOOBB010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50788_length_2325_cov_4.252863"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="PLT31_08880"
     CDS             complement(<1..762)
                     /locus_tag="PLT31_08880"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HPW95284.1"
                     /translation="MSKIIKKILALSLFASAMFATSAMAAPGLTISGTDFMYNGKKIF
                     FSGTNLAWSDYNSDVGESPLNENAWRKAVEGTRAAGGNAIRWWLFNNMSQSPAIDETT
                     HLVTGLKASTIANMKKALDIAEEYGVMVSMCLFSHNLMEPNQWGIYANEKLDFTANQT
                     LFEDAGTTAFINNALIPVVEAIGDHNALMSWEVFNEPEGMTSECEGWTTEKMALAKIQ
                     KFTNKVAAAIHTINPDLLVSTGSVNIKFQKHWNDAA"
     gene            complement(1385..1663)
                     /locus_tag="PLT31_08885"
     CDS             complement(1385..1663)
                     /locus_tag="PLT31_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95285.1"
                     /translation="MKILFLLSFFLFFGCSDNSAASITNPTDSSSSSSITHSSYSSSI
                     TNPASSFSSYSSSSIAYSSLSAIISSSSENTSFQDLCVQFVNEYARLG"
     gene            complement(1834..2130)
                     /locus_tag="PLT31_08890"
     CDS             complement(1834..2130)
                     /locus_tag="PLT31_08890"
                     /inference="COORDINATES: protein motif:HMM:NF023295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HPW95286.1"
                     /translation="MQLVNFEEKAFYDILKELCVKYDFSYPEDKMMELAKVVKTLVDG
                     QAKFPDWNKRDDIKSALKVGLILLLDEFGYPPVERDEVYVEIFEQAENFKKNQQ"
     gene            complement(2141..>2325)
                     /locus_tag="PLT31_08895"
     CDS             complement(2141..>2325)
                     /locus_tag="PLT31_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95287.1"
                     /translation="TWLMTAQEQTAVLAVSLKAAIGRRPRSLVTSRGSSTSAMAASTT
                     TLSAPVDGFGLFGVLI"
BASE COUNT          725 a          437 c          453 g          710 t
ORIGIN      
        1 agccgcatcg ttccagtgct tttgaaactt gatattcaca ctaccagtag aaactagaag
       61 atctggattg atcgtgtgga tggcggcagc aactttgttc gtaaacttct ggattttggc
      121 aagagccatc ttttccgttg tccagccctc gcattcactt gtcatacctt caggttcatt
      181 gaacacttcc cagctcatga gcgcgttgtg atcgccaata gcttctacaa caggaataag
      241 ggcattgttg ataaacgcag tggtgcctgc atcttcaaag agagtttgat ttgcagtaaa
      301 gtctagtttt tcgtttgcgt aaatgcccca ttgatttggt tccattagat tgtgactaaa
      361 aagacacatc gaaaccatca cgccgtattc ttcagcaatg tcaagagctt ttttcatatt
      421 ggctatagta cttgctttaa ggcccgttac taggtgagtg gtttcatcaa tggcagggct
      481 ttggctcata ttgttaaaga gccaccagcg aatagcatta ccgcctgccg cacgagtccc
      541 ttcgaccgct ttacgccaag cgttttcatt gagtgggctt tcgcctacgt cagaattgta
      601 gtcgctccaa gcgaggtttg taccagaaaa aaagattttc tttccattgt acataaagtc
      661 tgtaccgctt atggtaaggc ctggcgcagc catggctgag gttgcaaaca tagcgctcgc
      721 gaaaagcgaa agagcgagaa ttttcttgat tattttactc attattactc caatcaaatg
      781 tttatagaaa acataaattt actaaaccca aatataactt ttcttattca tatttaatag
      841 aaagacgttg tgaatcattc aattttttgt aatgaaaaca aaaaagaggc ttaaatccat
      901 tttgtttttt aacaaaagct caaaacataa agcaaactct ttgaaatcta aagcgattgc
      961 cgtttttatt aaataaaaac aacattatga cgaaaaacaa cccttagaaa cactttttta
     1021 atgattcttt gaataaagac attttactta gcatgaaaaa taatgaatga ctcaaaaaaa
     1081 atcagagaga gatgtaaaat cgttttttga tcaataaggc gtattctatt ttggaatatt
     1141 tttattagaa tgattaaatg taaaaaaaaa gttgcattag agggttttag aatagtatat
     1201 tgacattagt gcccttcgtc agtcctggtt tagagaaggg aagctggatc aaatcacggg
     1261 gtaggattga cgaagggcat gtttttccca cctctgggta aagataaaag tcgggctttt
     1321 atctttaccc ttttttttag gcaaaaaaca gcctttttag gccaaaatca atagaaattc
     1381 atgtttatcc aagccgtgcg tactcattca caaattgaac gcataagtct tggaaagaag
     1441 tgttttctga actcgatgaa atgatcgctg ataaagaaga ataggcaata gaagatgaag
     1501 aataagaaga aaaagacgac gctggattag taattgatga agaataagag gaatgggtta
     1561 ttgatgaaga cgatgatgaa tccgttggat tagtaattga tgctgccgaa ttatcactac
     1621 aaccaaaaaa taaaaagaat gataataaga ataagatctt cataaaagac ttctattaaa
     1681 agagaatcat taagattaat ataaactaag ccctaaccga ttccaaatat tttcaatccg
     1741 cctattggta aacttttgta gacgttttgg gttgttggtg attaataggt ggaattgact
     1801 tgggattgtt ttaaggggag aggtgggaga ttttcattgt tgattttttt tgaagttttc
     1861 agcttgctca aatatctcta cgtatacttc gtctctttct actggtggat agccaaattc
     1921 atctagtaat aaaatcaagc ctaccttcaa agcagatttt atatcatctc ttttattcca
     1981 gtcagggaat tttgcctgcc catccacaag agttttaacc acttttgcca attccatcat
     2041 cttatcctct ggataagaaa aatcgtattt aacacagagt tctttcaaaa tatcgtaaaa
     2101 ggctttttct tcaaagttta ctaactgcaa gactaccggc ttagattaaa accccgaaca
     2161 gcccgaaccc gtctactggc gcgcttagcg tcgtcgtgct ggccgccatt gccgaagtgc
     2221 tggagccgcg cgaggtcact agtgacctcg gacgacgacc aatagctgct ttcagcgaaa
     2281 ccgccaacac cgctgtttgc tcctgtgccg tcatcaacca ggtta
//
LOCUS       DAOOBB010000247         2246 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_53178_length_2246_cov_4.140575, whole genome shotgun sequence.
ACCESSION   DAOOBB010000247 DAOOBB010000000
VERSION     DAOOBB010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53178_length_2246_cov_4.140575"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..>2246)
                     /locus_tag="PLT31_08900"
     CDS             complement(44..>2246)
                     /locus_tag="PLT31_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95288.1"
                     /translation="KSNKNQSLELSTNFYSDKWNRTDLDVFSLSYTDNYHSLKLGDFY
                     LASGEVFLEGLPVFGANYELSLLKNKEDHPLFTFGGFFGESQKPFIKGIRNPDIYKDW
                     IDEGELKAQRLAWGGDFKWDPLRRFNAHFGVLFSDDEIDDPLLRDGDRATAITADPLQ
                     STMALYAEGNWLFFPGDIELNGQIALGRSDTADVIAQRAINQVFEDAGLSTSSFTTLR
                     QLMQNSNLIQTLSREEMTEIFGNYTTLTNSEMRDSLSNLIQEAKKKRDDFKKDTEDSR
                     TMGLDFTSQNVAIGASLFWNIKKTTLFGHIHYVGENYYSPGSPNQLSDIREFGGYLKQ
                     EVSPKWTFKFSYELDVENAANDTALNIFGLSEGTRLGLFDEPDSLYLKEHELDIDRTK
                     YRHDIGLENTFSINKNTDLFFNYQFEYQRQHRPYRLRGLYDAESGIFNDPWFRASTNS
                     DTTNLVYYRDTILVDSARWSDYQKLTTEPYIASDFEEHFYKHTFDLGFSFSFWKSELR
                     INGIWTTMLDGSFFNNDSLIEDIDFSDSTWSKLGYYFNGANYFEHRYPISLQTTNSKF
                     TNRLTLTPRFKNYERNEMKETEWSLSNALKVPLFSKFITLGLSVEMKTFRFSWKEADY
                     TLQDTLTQEKFHYYRVENNNIVATNNPIATDSEVGSHLNKIENGYELISELEEKTEKE
                     NDFIVEVSAKINHTKQLFSEWICRGEVFDRPDQISNEYKDLYVGFTINYSF"
BASE COUNT          708 a          392 c          393 g          753 t
ORIGIN      
        1 aagtcaacag gaagttctct aaattgatat tttggcatta gaattaaaag gaatagttta
       61 tggtaaaacc tacataaagg tctttatatt catttgatat ttgatctggt ctatcaaaga
      121 cttctcctcg gcaaatccat tcactaaaga gctgttttgt gtgattgatt tttgctgaaa
      181 cttctactat aaaatcattt tctttctctg tcttttcttc taactctgat attaattcat
      241 agccgttctc tattttattc aaatgagagc cgacttcact atctgttgct ataggattgt
      301 tggtggcaac gatgttgtta ttctctacac gataataatg aaatttctct tgagttaaag
      361 tatcttgaag cgtgtaatcg gcctctttcc atgagaatcg gaaggttttc atttctacag
      421 ataagcctag agtaatgaat ttagagaata gaggcacttt taaagcattt gataaactcc
      481 attcggtttc cttcatttca tttctttcgt aattcttaaa acgaggggta agagttaagc
      541 gattggtgaa tttagaattg gttgtttgta aagaaatggg atatctatgc tcgaaataat
      601 tcgctccatt aaaataatag cctaatttac tccaggtgct atcagaaaaa tcgatgtctt
      661 caattaaaga atcattattg aaaaaagaac cgtcaagcat agttgtccaa ataccattga
      721 tccttaattc gctcttccag aaagaaaaag aaaaacctag atcaaaagta tgcttataaa
      781 aatgttcttc aaaatcagag gcaatatagg gctcagtagt gagcttttga taatcactcc
      841 aacgagctga atctacaaga atggtgtctc tataataaac aaggtttgtt gtatcactat
      901 ttgtggatgc tctaaaccaa ggatcattga aaataccgct ttcagcatca tataaacctc
      961 ttagacggta tggacgatgc tgtctttgat attcaaattg gtaattaaag aataagtcgg
     1021 tatttttgtt gatgctaaat gtgttctcta atccaatgtc atggcggtat ttagtacggt
     1081 cgatatctaa ttcatgctct tttagataaa gagaatctgg ttcatcaaat aaacctaatc
     1141 gagtcccttc acttaaacca aaaatattaa gggctgtgtc gttagcggca ttttctacat
     1201 ctaattcata gctgaatttg aatgtccatt ttggtgatac ttcttgtttt aaataaccac
     1261 caaattcgcg aatatcactc aattgatttg gagagcctgg gctataataa ttttcaccta
     1321 cataatgaat atgtccaaat aaagtagtct ttttaatatt ccaaaagaga gaggcgccta
     1381 tggctacatt ttgactagta aagtcgagcc ccatagttct agaatcctct gtatcttttt
     1441 taaaatcatc tcgctttttc tttgcttcct gaataagatt tgagagcgaa tctctcattt
     1501 cactattcgt tagagtggta taattaccaa atatttcagt catttcttct ctagaaagag
     1561 tttgaatcag gttggagttt tgcatgagtt gacgtaaggt ggtaaatgaa gatgtggata
     1621 aaccagcgtc ttcaaaaacc tgattgatcg ccctttgagc gatgacatct gcagtatcag
     1681 atcgtccaag ggcaatttga ccatttaact caatatcacc agggaaaaat aaccagttgc
     1741 cttctgcgta taaagccatg gtgctttgta atgggtcagc agtaatggct gtagctcgat
     1801 caccatctcg taaaagagga tcgtcaattt catcatcaga aaatagaact ccaaaatggg
     1861 cattaaaacg acgtaatgga tcccacttaa agtcaccacc ccaagctaag cgctgtgctt
     1921 ttaattcacc ttcatcgatc cagtctttat aaatatctgg atttcggata cctttaatga
     1981 atggtttttg agactctcca aaaaatcctc caaaggtaaa taagggatga tcttctttat
     2041 tttttagaag agaaagctcg tagtttgccc caaatacagg tagcccttct aaaaatactt
     2101 ctcctgaagc taaataaaag tcacccaatt ttaaggagtg gtaattatca gtgtaactta
     2161 atgaaaaaac atctaaatcg gttcgattcc atttgtctga atagaaattt gtagatagtt
     2221 ctaaagactg gtttttattg cttttg
//
LOCUS       DAOOBB010000248         2216 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_54117_length_2216_cov_4.149005, whole genome shotgun sequence.
ACCESSION   DAOOBB010000248 DAOOBB010000000
VERSION     DAOOBB010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54117_length_2216_cov_4.149005"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2215
                     /locus_tag="PLT31_08905"
     CDS             <1..2215
                     /locus_tag="PLT31_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPW95289.1"
                     /translation="PVSGATSYNVYYSGAGVSEQKLDAPLIRKYGSYYRADALGLAPG
                     SYTLKVVPVTNGVEGTGAVTSTVTVKAHDRSGFAFSNNKVPGAYNKDGTPKNGAVILY
                     ITENTKNTISLEVTGASANPCVGLQTILDGFKKGKDSRPLIVRLIGSISDMSYMISGD
                     VVIENSNNASGSITIEGVGEDAVANGWGIRLKNASNIEVRNLGIMNVNSGEGDNLGLQ
                     QNNEYVWIHHNDFFYGDAGSDSDQAKGDGALDCKKSTYVTFSYNHFWDTGKSNLLGLS
                     EGTTEGLYITYHHNWYDHSDSRHPRVRFYSAHVYNNYYDGISKYGIGSTNGSSIFAEG
                     NYFRNCKYPMLTSMQGSDVWDESKGANDYGEMPTFSKEDGGTIKAYDNYIEGAKRFVA
                     YGDTKFSGSTVDFDAYVVTSKSTSVPSSVKSVYGSNTHNNFDQNTSIMYSYTAESPTS
                     AKTTVMAYAGRMNGGDFKWTFNNAVDDTAYSVNTALKSALIKYTTSLIQVQGESESVI
                     TSSNSNESSSSNSNGSSSSQEPLSSSSSNSGSLINGDIVHNFTLDGTSSNHFNITGNL
                     STSKGTVTYGNLSLTQCLKMESSTQISFSIENAAELTLVFNPDFSGSVKINGSSYSSN
                     NGIATISLESGDYTITKGESSNLFYISISFEKTTPIKRIVHLHNRVYFDSYANALYIP
                     ESVGITQINIVSVNGDHLKMKASGSVVDLNHLRSGVYIVRVKTDLGWIQNKIYKK"
BASE COUNT          680 a          362 c          469 g          705 t
ORIGIN      
        1 ccctgtcagt ggggctacta gctataatgt ctattatagc ggcgcaggtg taagcgaaca
       61 gaagctagac gccccattga ttcgtaaata tggttcatat tatagagccg atgctttagg
      121 gttagctcct ggttcctata cattaaaagt ggttcctgtg actaatggcg ttgagggaac
      181 aggggctgta acatctacag tcactgtgaa agcgcatgat cgttctggtt ttgctttctc
      241 taataacaaa gttccaggcg catataataa agatggtact ccaaaaaatg gggcagtcat
      301 tctttatatt accgagaaca caaaaaacac aatctcttta gaggtgacag gggcgagtgc
      361 aaacccttgc gtgggtttac agaccatttt agatgggttt aagaaaggaa aagatagccg
      421 tcctttaatt gttcgtttaa taggttccat tagtgatatg tcttatatga tctctggaga
      481 tgttgttata gaaaattcaa ataatgcttc tggttctatt acaattgaag gtgttggtga
      541 agatgctgta gcgaatgggt ggggtattcg cttaaagaat gcaagtaata ttgaagttag
      601 aaatcttggg attatgaatg tcaatagtgg tgaaggtgat aatttaggac tacagcaaaa
      661 caatgaatat gtttggattc atcataatga tttcttttat ggagacgctg gatcagattc
      721 tgaccaagca aaaggcgatg gtgctcttga ctgtaaaaaa tcaacatatg tgacgttctc
      781 ttacaatcat ttttgggata caggaaaatc gaatctctta ggtttaagcg aaggaactac
      841 agagggcctt tatattactt atcatcacaa ttggtatgac cattccgatt cacgccatcc
      901 aagagttcgt ttttattctg ctcatgttta taacaattac tatgatggca tttcgaagta
      961 tggaattggc tctacaaatg gttcttctat ttttgccgag ggcaattact tccgtaactg
     1021 taaatacccg atgttaactt ctatgcaggg atccgatgtt tgggatgaat ccaaaggggc
     1081 taatgactat ggtgagatgc ccactttctc aaaagaagat ggtggaacga taaaagctta
     1141 tgataattat attgaaggcg ctaaacgatt tgtggcttat ggagatacaa agtttagcgg
     1201 atccactgta gattttgatg cttatgtggt gacgagtaaa agcacttctg tgccttcttc
     1261 tgtaaagtcg gtttatggtt ctaataccca taataatttt gatcaaaata cttcgattat
     1321 gtattcatat acggctgaat ctcctacatc agcaaaaacg accgtcatgg cttatgcggg
     1381 ccgaatgaac ggaggtgatt ttaagtggac atttaataat gcggttgatg atacggctta
     1441 tagtgtaaat acggctttga agtccgcttt aatcaagtat actacgtcat tgattcaagt
     1501 gcaaggtgaa agcgaaagtg taatcactag ttcgaattcg aatgagtctt ctagttcaaa
     1561 ttcaaatggg tcttctagtt cacaagaacc tttgtctagc tctagctcaa actcaggttc
     1621 tttgattaat ggagatatag ttcataactt tacgttagat ggtacttcta gtaatcactt
     1681 taacattact ggaaatttat ctacttcaaa agggactgtt acttatggaa atctctcttt
     1741 aacacaatgc ttaaagatgg aatcgtctac ccaaatttca ttctccattg aaaatgcagc
     1801 agaacttact ttggttttca atcctgattt ctcaggatca gtaaagatca atggatcttc
     1861 ttattcgagt aacaatggaa tagcaacaat cagtttagag tctggagatt atacgattac
     1921 taaaggggag agttcaaatc ttttctatat cagcatctct tttgaaaaaa cgacccctat
     1981 aaagagaatt gttcatcttc acaaccgagt gtattttgat tcttatgcga acgcccttta
     2041 tattccagaa tcagtaggca taacacaaat aaatattgtg agtgtaaatg gtgatcatct
     2101 aaagatgaaa gcgagtggat ctgtggtgga tttgaaccat ttgcgatctg gggtatatat
     2161 tgttagagtc aaaactgatc ttggttggat tcaaaacaaa atttataaga agtaat
//
LOCUS       DAOOBB010000249         2210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_54302_length_2210_cov_4.834803, whole genome shotgun sequence.
ACCESSION   DAOOBB010000249 DAOOBB010000000
VERSION     DAOOBB010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54302_length_2210_cov_4.834803"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1527)
                     /locus_tag="PLT31_08910"
     CDS             complement(<1..1527)
                     /locus_tag="PLT31_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HPW95290.1"
                     /translation="MPEKRLFLLDSYALAFRMFYAYYKSPLINSAGLNVSMVHGYWGA
                     VLRILDTHKPTHFAIVRDVSAPTFRHELFPEYKANRGPMPEEMVAQMPLLGETLAASG
                     ITLLSEPGFEADDVMASVAVAAEKAGFDQIFIVTKDKDMAQMVNDKIHLFHLEKGAEG
                     IDFGPEQVLEKYGVPPSKIRDYLALMGDSSDNIPGVPKIGPKTAAQLLSEFDNIDNLY
                     LNLDKITKKGLHENLRENKEKAFLSRELVTLQSTRGFSGTLEQLEYTGVNVNALTEIF
                     QKNEINSLLRLLKPISDRSSQKASSHQESLIEEKEPDYVLVDSIEKWESMKLEFESKK
                     IIAVDTETDSLSPITTNLVGLCLSCDEAKGYYIPLAHTDELGLELNNFDKKLVSDWFL
                     SLWNNPQIQWVFHNAKFDLHVLKRSFGIHLNVKILDSLIAAWMLSPGDMGLGLDEQVR
                     RHLHHEMIPIENLIGRGKNQITFNRVPVNQAYTYGAEDAVYTLRLWNKLLPLIQERDY
                     E"
     gene            complement(1626..>2210)
                     /locus_tag="PLT31_08915"
     CDS             complement(1626..>2210)
                     /locus_tag="PLT31_08915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HPW95291.1"
                     /translation="KALFKYQKAVCIGLGLSQKPEVKQVVTQLLPTFTSPVILDADAL
                     NALDGNPDLLLSLNVDAILTPHVREWERLFGALPSKMEDVIKVVKEKASQYKVTLVLK
                     GVPTCIASPKGEVYLVGKANSGLAKGGSGDVLAGIILALLAQGLSTLEAAILGTWVHA
                     EAGQRTRKRLGAFSMLPTDLIDSLPSIFMGESSK"
BASE COUNT          715 a          459 c          350 g          686 t
ORIGIN      
        1 ttcatagtct cgttcttgaa ttaagggtaa taatttattc cacaagcgta aggtataaac
       61 cgcatcttct gcgccgtaag tataagcctg atttacaggc acccgattaa acgtaatctg
      121 attcttgcct cttccaataa gattttcaat tggaatcatt tcatgatgta aatggcgacg
      181 cacttgttca tcaaggccaa gccccatatc acctggggat aacatccaag cagcaattaa
      241 agaatcgaga atttttacat ttaaatgaat gccaaaactt ctttttaaaa catgtagatc
      301 aaattttgca ttgtgaaaca cccattgtat ttgaggatta ttccataaag acaaaaacca
      361 atctgacacc aattttttat caaaattatt taactctaat ccaagttcat ccgtatgcgc
      421 aagaggaatg taatagcctt tcgcttcatc acaagaaaga cataatccca ctaaatttgt
      481 agttattgga gataaggaat ccgtttctgt atctactgca attatttttt tggattcaaa
      541 ttccagtttc atggattccc atttttcaat cgaatccaca agaacataat caggttcttt
      601 ttcttctata agagattcct gatgactaga agccttttga ctcgaacgat ccgaaatagg
      661 ttttaataaa cgaagcaaac tattgatttc atttttttga aaaatctcag ttaaagcatt
      721 cacgtttaca ccagtatatt ctaactgttc taaagtgcca gaaaaaccac gagtactttg
      781 taacgtcact agttcacgag aaagaaatgc tttttcttta ttttctctta agttttcatg
      841 caatcctttt tttgtaatct tatcgagatt caaatataag ttatcgatat tatcaaattc
      901 gcttaacaat tgagcggccg ttttaggccc aatttttgga acgccaggaa tattatccga
      961 actatcgccc attaaagcga ggtaatctcg aatttttgat ggaggtactc catacttttc
     1021 taaaacttgc tctggaccaa aatcaatacc ctcagctcct ttttctaaat gaaataaatg
     1081 aattttatca tttaccattt gagccatgtc tttatcttta gtcacaataa aaatttgatc
     1141 aaagcctgct ttttctgcgg ccacagcgac acttgccatg acatcatctg cttcaaaacc
     1201 gggttcagaa agtagggtaa ttccacttgc agcaagtgtt tctccaagca atggcatttg
     1261 ggcaaccatt tcttctggca ttggtccacg attcgcttta tattctggaa ataattcgtg
     1321 acgaaatgtc ggtgcactca catcgcgaac tatggcaaaa tgcgttggct tatgagtatc
     1381 taaaattcga aggactgctc cccaatagcc atgcaccatc gagacattca acccagcaga
     1441 attaattaat ggtgatttat aatacgcata aaacatgcga aaagctaaag cgtaagaatc
     1501 taataagaac agtctttttt caggcataaa aagaatgtag aaaaataatc cctcatgtca
     1561 ttttaaaaac gcttaaagat tctcttcctc aagaaatcgc ccaaaaataa aaaaaacgta
     1621 atattttatt tggatgattc acccataaag atacttggaa gcgagtctat tagatctgta
     1681 ggcaacatac taaacgcacc taatcgcttt cttgtccttt gccctgcttc tgcatggacc
     1741 caagtgccaa gaatcgccgc ttctaaagtt gacaaacctt gagctaaaag tgcaagaata
     1801 atcccagcta aaacatctcc agagccccct tttgcaaggc cagaatttgc tttacctacc
     1861 aaataaactt ccccttttgg cgaagcaata caagtaggaa cgccttttaa aacaagagtc
     1921 actttatatt gagacgcctt ttctttaacc acctttataa catcttccat ctttgatggt
     1981 aaagccccaa aaagccgttc ccattcacga acatgtggtg ttaaaatagc atccacattt
     2041 aaggagagaa gtaaatcagg atttccatct aaagcattta aagcgtctgc gtctaaaatc
     2101 actggagatg taaaagtagg taacaattgt gttaccactt gttttacttc tggcttttga
     2161 gaaagaccaa gcccaataca aaccgccttt tggtatttaa aaagtgcttt
//
LOCUS       DAOOBB010000250         2193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_54813_length_2193_cov_5.164172, whole genome shotgun sequence.
ACCESSION   DAOOBB010000250 DAOOBB010000000
VERSION     DAOOBB010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54813_length_2193_cov_5.164172"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..1067)
                     /locus_tag="PLT31_08920"
     CDS             complement(21..1067)
                     /locus_tag="PLT31_08920"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HPW95292.1"
                     /translation="MRLLTFILIFIGVSNAQLLDVSEQASAENKIQKVSVEGHVNMDE
                     RSILSRVSIRAGQSLSPMALSEKVQSSVSALYESGFFDDVSAWIEYTGEANDVNLVFQ
                     VKELPALDTLIIEGNDEISVEDLNLKVSLIQGQVYSKSALERDRQNMLSHYRSEGYLL
                     AEIGIKEIPISDNENRVIFSIREGEKVKVRQIVFSGNDHIPAENFTDHMQTKVTRWWG
                     EGEFKEHIFEADRDSILNVARHFGFLDAEITNFEAEYLPDSTCKFYLGQTVRPDTKMT
                     LLFAQMNTAIFDKENPMHLLAGQTEDLSKHYFRQFRNNYNNKARALPVPVVRDEETAV
                     RILNKIIAYKDVCL"
     gene            1285..>2193
                     /locus_tag="PLT31_08925"
     CDS             1285..>2193
                     /locus_tag="PLT31_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HPW95293.1"
                     /translation="MKSSCWNDQNGLFLPEMSLDFMPVERLKQVQLQRLQATVSLAYE
                     KVPLFRSRMDEKKVKPSDIKSLKDIALIPFTMKKDLRDTYPYGLFAAEMKQVVRLHAS
                     SGTTGKPIVVGYTREDMEVWSQVVKRGLLATGYTKDDIVQNFYGYGLFTGGLGVHGGL
                     EALGAAVIPVSGGNTERQIMIMKDFEVTAIAGTPSYFVRIIDVAERMGIDLRTLKIKR
                     GLFGAEPWSDGMRDYIEERSGIKAYDIYGLSEIIGPGVGCECECQNGIHIFEDHFYPE
                     IIDPETLEPLPDGQEGELVISTLSKQA"
BASE COUNT          693 a          430 c          409 g          661 t
ORIGIN      
        1 gcaaagcgca gttttgcaaa ttagagacag acatctttat atgcaatgat tttatttaaa
       61 atacgtaccg ctgtttcttc atcccgaact actggtacag gtagagcacg agccttgtta
      121 ttgtaattat ttcggaattg cctaaaatag tgtttggaaa gatcttctgt ttgccctgct
      181 aagagatgca ttggattttc tttatcgaaa atagccgtat tcatctgagc aaaaaggaga
      241 gtcattttag tatcaggacg aaccgtttgc cctaggtaaa atttacatgt ggaatcaggc
      301 aagtattcag cctcgaagtt agtaatttcg gcatctaaaa agccaaaatg acgagctacg
      361 tttaaaatag aatcgcgatc tgcttcaaaa atatgttcct taaactctcc ctcaccccac
      421 caacgagtga ccttggtttg catatggtcc gtaaaattct ctgctggaat atggtcattg
      481 ccagaaaata caatttggcg aaccttgacc ttttcccctt cacgaataga aaaaatgact
      541 ctattttcat tatcagaaat tggaatttct ttgatcccta tttcagcaag aagataacct
      601 tctgaacgat aatgagaaag catgttctga cgatctcttt ctaaagcact cttactgtaa
      661 acttgtcctt gaatcaaact gaccttcaaa ttcagatctt ccaccgaaat ctcatcatta
      721 ccttctatga ttagagtatc aagagctggt aattctttta cttgaaagac aagattcaca
      781 tcatttgctt ctcctgtata ttcaatccaa gcactgacat cgtcaaaaaa gcctgattca
      841 tacaaagccg aaacactaga ttgaaccttt tccgatagag ccattggaga gaggctttgc
      901 cccgcccgaa tactcactcg gcttaaaatg gaacgttcat ccatgttaac atgaccttcc
      961 acagaaacct tctgaatttt attttcagca gaagcctgtt cagacacgtc taaaagctgt
     1021 gcattagata caccaataaa gatcaaaata aaagtaagta atcgcaaaaa cagaggaaac
     1081 ccgcttcaaa aagtttttcg gctcaaaaat acaaaagtaa atgattcaga ccagttgtta
     1141 aaagaaaaag acctcaaaaa aagacctttt tttaaagcgc ccttttttaa cgattcaaaa
     1201 cgtccttttt tatttaagaa agctccattt tttatttaga ttaaggacat atttttcttt
     1261 ttgcattacg acgaggtttt tttaatgaag agtagttgtt ggaatgacca gaatggtctt
     1321 tttctcccag aaatgtcact tgattttatg cctgtagaaa gactaaaaca agtacaatta
     1381 cagcgtttac aagcaacagt atctttagct tatgaaaaag ttcctttatt ccgttcaaga
     1441 atggatgaaa aaaaagtaaa gccctctgat ataaaatcat taaaagatat cgctctgatt
     1501 cctttcacca tgaaaaaaga tcttcgggac acctatcctt acggtctttt tgccgcagaa
     1561 atgaaacagg ttgttcgact acatgcttct agcggcacta caggtaagcc tatcgtcgtt
     1621 ggctatacac gagaagacat ggaggtttgg tcacaagtcg tcaaacgcgg cttgctcgct
     1681 acaggctaca cgaaagatga tattgttcag aatttttacg gttatggtct ttttactggt
     1741 ggcttaggag ttcacggtgg cttagaagct ttaggcgcag cagtgattcc tgtcagtggt
     1801 ggaaatacag aacgtcaaat catgattatg aaagactttg aagtcactgc cattgctggc
     1861 actccaagtt attttgttcg aatcattgat gttgcagaaa gaatgggcat agatttaaga
     1921 actctcaaaa ttaaaagagg cctctttgga gctgaaccat ggtcagatgg catgcgcgat
     1981 tatatcgaag aacgttctgg cattaaggcg tatgatattt atggtttatc tgaaatcatt
     2041 ggtccaggcg tcggttgcga gtgtgaatgc cagaatggaa ttcacatttt tgaagatcac
     2101 ttttacccag aaattattga ccctgaaact cttgaacctt tacccgacgg tcaagaaggg
     2161 gaattagtca ttagtacctt aagcaaacaa gct
//
LOCUS       DAOOBB010000251         2153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_56227_length_2153_cov_3.844137, whole genome shotgun sequence.
ACCESSION   DAOOBB010000251 DAOOBB010000000
VERSION     DAOOBB010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56227_length_2153_cov_3.844137"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="PLT31_08930"
     CDS             <1..1017
                     /locus_tag="PLT31_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPW95294.1"
                     /translation="GQDGDWEEALEIKDNPVMARGVTIDFTGKSWFRMVSTEESGPLL
                     EIEAFDVSNGGNDTWFFMGTSISQMGIKQQDDDQSKTMADWIHEKYPQYTPAMLRGGI
                     GCINSTEVVAHLSEYLAYAGNVKFWAIEMGTNDAWGGGDWNLETFRKNMQTIIDSAKA
                     HNIVPILARIIATDSAKAQWQINPKFLEAIDSLTVQNKLPKGPDLYTYFREHPELLAS
                     DGIHPNGETGGGHMIHQLWAEALYPLYENGFDNVEEDTKIIKKTISKGPQIFVQGRTI
                     KIAGVVEPKSQVMLLNAIGQVLQTHTIKRTENFELKTNTPTGNYFVVLRGQNTAITTQ
                     VTIK"
     gene            1566..>2153
                     /locus_tag="PLT31_08935"
     CDS             1566..>2153
                     /locus_tag="PLT31_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPW95295.1"
                     /translation="MKSRYFGMAVVALSTFVFVNCGNESGPVDPNVIGELLSSSSEEV
                     IVPPLSSNSEIPVDLSSSLGEVPLSSEGFPLSSAEVPMSSEAIVVSSSSEVIIPSSSS
                     EVVVSSSSEVVVPTGIYLANDKDENKNYMQVEYKINTGFDGGGILAYPKNLSTTQKHG
                     IVVWGPGGGTPPSSYEGIIRRLASHGFVVIALKESP"
BASE COUNT          649 a          396 c          447 g          661 t
ORIGIN      
        1 ggccaagatg gcgattggga agaagcttta gaaatcaagg ataacccagt catggcacga
       61 ggtgtgacta ttgattttac aggaaaatct tggtttcgca tggttagcac agaagaatct
      121 ggccctttat tagaaatcga agcttttgat gtgagtaatg gtggtaatga cacctggttt
      181 tttatgggaa caagcattag ccaaatgggt atcaaacaac aagatgatga ccaatctaaa
      241 accatggcag actggattca tgaaaagtac ccacaatata caccagcgat gttacgaggt
      301 ggaatcggtt gtattaatag cacagaagtc gttgctcatt taagcgaata ccttgcttat
      361 gcagggaacg taaaattttg ggccattgaa atggggacta atgacgcctg gggtggtggc
      421 gattggaatt tagagacttt ccgaaaaaac atgcagacta ttattgattc tgcaaaggct
      481 cataatattg ttcccatttt agctcgaatc atcgcgaccg attctgcaaa agcccagtgg
      541 caaattaacc ctaaattttt agaagcaatt gatagcttaa ccgttcaaaa taaattgcct
      601 aaaggccctg atttatacac ttatttccga gaacacccag aattattagc aagtgatggc
      661 attcatccta atggagaaac tggtggaggt cacatgattc accaactctg ggccgaagcg
      721 ctttacccac tgtacgaaaa tggctttgat aatgtagaag aagatacaaa aattataaaa
      781 aagaccattt caaaagggcc tcaaattttt gtacaaggtc gcacgatcaa aattgcagga
      841 gttgtagaac caaaatctca ggtgatgctt ttgaatgcga tagggcaagt tctacaaacg
      901 catacaataa aaagaacaga gaatttcgag ctcaaaacaa acacccccac agggaattac
      961 tttgttgtgt tacgtgggca aaatactgcc attaccaccc aagtgacaat caagtaaaaa
     1021 aacgcacggc tacgatgagc gacgtcgtat cttgccgtat aaaagttttt tacaaacaaa
     1081 aaaagagctc ttccttaggg aagacccttt tttttaatga taagctttaa ttaaatacaa
     1141 ccattaaatt ctaaatgatt aattaaagtg gttccagata tagtgtattt tgcagtagca
     1201 gatgcatctg acttgtagac aaaataatga tttgcattct tgctacaaat gcaccgctat
     1261 tgtgtaatgt gaatgatttg atttcatttg actgtcccat ttttatctcc ttatgtttga
     1321 ttgttgggat tgttaagttt gttttacaaa gatatttccc agcagtatta ttttcccatt
     1381 acccaaaggg cttttttttt gggataacca tcgtttttaa gcaaaaaagc ttattcactt
     1441 cctcttgctt tttctaatga agaatatgga catattgtcc ataagaacac tcttgtattg
     1501 gatagttttg catttgctgt aaagtagttt atatgtggtt ttggtataaa aaaaggagtt
     1561 agaagatgaa gtctagatat tttggtatgg ctgtggttgc tttaagcacc tttgtctttg
     1621 taaactgcgg taacgaatct ggtcctgtag accctaacgt cattggtgaa cttttatcta
     1681 gttccagtga agaagttatt gttccacctc tttctagtaa tagcgaaatt ccagtggatc
     1741 tttctagttc tcttggagaa gttcccttat cttccgaagg attcccttta tcttccgcag
     1801 aagttcctat gtcttccgaa gcgatagtgg tttccagctc ttctgaagtg atcatccctt
     1861 cttcctcttc cgaggttgtt gtaagttcaa gcagcgaagt ggttgttcct acaggtattt
     1921 accttgctaa cgacaaggat gaaaataaga actatatgca agtggaatac aaaattaata
     1981 ctggctttga cggcggaggc attcttgcct acccgaagaa tctttctact acccagaagc
     2041 atggtattgt ggtttggggc ccagggggag ggaccccgcc aagttcttac gaaggtatta
     2101 tccgtcgttt agcttctcac ggctttgtgg ttattgctct taaggaatct cca
//
LOCUS       DAOOBB010000252         2150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_56320_length_2150_cov_3.507399, whole genome shotgun sequence.
ACCESSION   DAOOBB010000252 DAOOBB010000000
VERSION     DAOOBB010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56320_length_2150_cov_3.507399"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="PLT31_08940"
     CDS             <1..716
                     /locus_tag="PLT31_08940"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPW95296.1"
                     /translation="ELRHLFAMPIAIEENAEVADLAISLESKIPNRYSQHEKELLKTI
                     AGVAGFAYERARAFEKGKDLATKDGLTGLINHRTLHEQLRTEKLRAERQKINIGVMMM
                     DIDHFKSVNDTYGHPAGDEVIKGIASTIREEIRSEIDIVARYGGEEFVVALIDTTPEG
                     LKDTAERIRLAIEKKAFDIHQSSPLKITVSIGAYLVLPEFRDMKQALKQADQALYKAK
                     NSGRNQVFVYQKEEDVHLD"
     gene            700..>2150
                     /locus_tag="PLT31_08945"
     CDS             700..>2150
                     /locus_tag="PLT31_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546804.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HPW95297.1"
                     /translation="MFTWIDSLVLVSYLLLSVAIGLWVSRGKKSFKDYMFGGGSMPWV
                     AVGISLIATSVSATTFLGAPADVFGADMTFLMFQFGAFISIFVIGFLFIPQFKKSGIQ
                     SAYELFEMRFSRSTRRLAAIFYCMHLLLRTGILLYAPSIVLSRIMGVDIRVAILISAI
                     IAIFYTWFGGIKAVIWTDVMQFTVFFGGGIVVLIFIAQDVGGFSSMVELASQHGKTKW
                     FDPSLDPSNARTLISAGLAYAVLETAIRGGDQQFVQRYLSCKSAADANRSSVLSMVLG
                     VLVSIIFYWVGAALFVYYKVAHVSTLPEGTGVNDVFPYFIVESLPQGLTGLIVAAICA
                     AAMSSLSSAINSLSNTTENDILCVASKNGQSVKRAKWWTVLWGVLGVGAAFIAASQQG
                     SLLKNALFFTGLFTGPLLAMFILAFFTRKMMSVCVIVGVLCGMASLTLFNGIPFMGIE
                     GPLTGILSWPWMPLISLSVTIVTAFLLHFILLTL"
BASE COUNT          557 a          396 c          491 g          706 t
ORIGIN      
        1 ttgaactcag gcaccttttt gcgatgccta ttgccataga agaaaacgcc gaagttgctg
       61 acttagccat tagcttagaa agcaaaatcc cgaaccgtta ttctcaacac gaaaaagaat
      121 tactcaaaac aattgctggg gtcgcgggtt ttgcttatga aagagcaagg gcgtttgaaa
      181 agggcaaaga tttagccaca aaagatggct tgacagggct tatcaaccat cggacgctac
      241 acgagcaatt gcggacagaa aagcttcgtg ccgaacgtca gaaaataaat attggcgtga
      301 tgatgatgga tattgatcac tttaagagtg tgaatgatac ttatgggcat cctgcgggag
      361 acgaagtcat caaaggtata gcttctacta tacgagaaga aattcgttca gagattgata
      421 ttgtcgcccg ttatggtggt gaagaatttg tggtggctct aatagacacc acaccagaag
      481 gattaaaaga cactgccgag cgtattcgtc ttgcgattga aaagaaggct tttgatatcc
      541 atcaatcgag tccattaaaa atcacggtca gtataggtgc ttatcttgtg ctaccagaat
      601 ttcgtgacat gaaacaagca ttaaaacaag cagatcaagc gctctataag gcaaagaata
      661 gtggacgaaa tcaagttttt gtctaccaaa aggaagaaga tgttcacttg gattgattcc
      721 cttgttttag tttcatactt gttattatca gttgccattg ggctttgggt ttctcgagga
      781 aaaaaaagct ttaaagatta catgtttggg ggcggttcta tgccttgggt ggctgtgggg
      841 attagtctca ttgccacttc tgtgagtgcg actacctttt taggggctcc tgcagatgtt
      901 tttggcgccg acatgacatt tttaatgttt caatttgggg cattcatcag tatttttgta
      961 ataggttttt tatttattcc tcagtttaaa aaatcgggaa tccagagtgc ctatgaatta
     1021 tttgaaatgc gcttttctcg ctccactagg cgtttagctg ccatattcta ttgtatgcac
     1081 ctactccttc gaacaggtat tttactatac gccccttcta tcgtattatc tcgcattatg
     1141 ggtgtagata ttcgagtggc gatattaatt tctgcgatta ttgccatttt ctatacttgg
     1201 tttggcggaa taaaagccgt tatttggacc gacgtgatgc aattcactgt cttttttggg
     1261 ggcggtatcg ttgttttaat ttttattgct caagatgtag ggggcttttc ttcgatggtg
     1321 gaactagcct ctcaacacgg aaaaaccaag tggtttgacc cttctttaga cccatcgaat
     1381 gcaagaactc ttatttcagc tgggttagct tatgcggtat tagaaacggc tattcgtggt
     1441 ggagatcaac aattcgtaca gcgttattta agctgtaaaa gtgccgccga tgcgaatcgt
     1501 tccagtgttt tgagcatggt tttaggtgtt ttagtctcta ttatctttta ttgggtgggt
     1561 gccgctcttt ttgtttatta caaagtcgct catgtgagta ctttaccaga gggcactggc
     1621 gtcaatgacg tatttcctta ttttattgtc gaaagtctac ctcagggcct cactggctta
     1681 attgtggcgg ctatttgtgc ggcggccatg agtagcttat cgagtgctat aaactcttta
     1741 agcaacacca ctgaaaatga tattctctgt gtagcctcaa aaaatggtca aagtgtaaaa
     1801 cgggctaaat ggtggactgt tttatggggt gttttaggcg taggagctgc ttttattgca
     1861 gcctctcaac aaggttctct cttgaaaaac gccttatttt tcacaggact ttttacagga
     1921 ccattactag ccatgtttat tttagccttt tttacccgaa aaatgatgtc ggtttgtgtt
     1981 attgtgggcg tcttgtgtgg tatggctagc ctcactttgt ttaatggcat accctttatg
     2041 gggattgaag gaccattaac aggtatatta agttggcctt ggatgcctct cattagtctt
     2101 tcagtgacga ttgtcacagc atttttactt cattttatat tgctaactct
//
LOCUS       DAOOBB010000253         2078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_59024_length_2078_cov_5.113693, whole genome shotgun sequence.
ACCESSION   DAOOBB010000253 DAOOBB010000000
VERSION     DAOOBB010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59024_length_2078_cov_5.113693"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="PLT31_08950"
     CDS             complement(<1..735)
                     /locus_tag="PLT31_08950"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPW95298.1"
                     /translation="MLVRFTVENWKSFKEPTSISFVAQKEKQHSNHIQYVPEYDLNVL
                     PISLIYGGNASGKSNLVKAIAFASQMITSTDFNPFGKFLLDYFRLSKECADKPSFFAF
                     DLIIDEKLFHYDFSLFNSEVTKETLICFNEKRKSLLFSRENGVYKFSPNIPKTSKEHF
                     QSTLPNKLALSSVLTKKLEGMDSIQKVFNWFRTSLMILSPESKLIQFNDRTLPVRNLS
                     HTLSLIDTGISRIDFEEITPQASGFSS"
     gene            complement(871..>2078)
                     /locus_tag="PLT31_08955"
     CDS             complement(871..>2078)
                     /locus_tag="PLT31_08955"
                     /inference="COORDINATES: protein motif:HMM:NF037202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95299.1"
                     /translation="LFVTNAIGMATNRDSWVYSFSEKKLAKNMKSCIEFYNSQRELYK
                     KFLKSGKKLEDIISNDPTKISWTVNLKKSLESDVPFNFIKNIRQTCYRPYEKQFLYFE
                     KDWIERPGINPKLFPTPDTQNLVICVKGIGDKDFSCIITDSIPDLQVVFNGQCFPLYY
                     YAEQSKSDLFSQELEKRDGISNWALAEAKRLYGSSKKITKENIFYYVYGFLHSKDYRE
                     TFKDDLKKSLPKIIFVSKYEDFCAFERAGRALANLHLNYEKSSDEAEKIAKTLGINIT
                     GNKLTKTTGQTYTPEDYKYFRIPDKMRFASKADKTKIFYNNSITIENIPERAYEYIVN
                     GKSAIDWILERYAVTQDKDSLITNDCNDWALEHQNPRYILDTLVSVINLSLKTMEIVN
                     GLPKLDFGE"
BASE COUNT          590 a          327 c          446 g          715 t
ORIGIN      
        1 gctactaaat ccactagctt gtggagttat ttcttcaaaa tcgatacgag aaatccctgt
       61 atcaattaga ctcagtgtat gagacagatt acgaacaggt aaagttctat cgttaaattg
      121 aatcagtttg ctttctgggc ttaatatcat taaggaagta cgaaaccagt taaaaacttt
      181 ttgaatagaa tccattcctt caagtttttt tgtcaaaacg ctggatagag ccagtttgtt
      241 tggtaaggtt gattggaaat gttcttttga tgttttaggt atattgggac tgaatttata
      301 aactccattt tctcgagaga aaagaagaga ttttcttttt tcattgaagc agataagcgt
      361 ttcttttgtt acttccgaat taaaaagtga aaaatcataa tggaataatt tctcatcaat
      421 aattaaatca aaggcgaaga agctcggttt atcagcacac tcctttgaca aacgaaaata
      481 atcgagaaga aatttgccaa acggattaaa atctgtcgat gtgatcattt gtgatgcaaa
      541 agcaatcgcc ttgactagat ttgattttcc agaagcgttt ccaccataga taagggaaat
      601 tggcaacaca tttaagtcgt attctggaac atattgaata tgattagagt gttgtttctc
      661 tttttgtgca acaaatgaga tagaggttgg ctctttaaag gacttccaat tttcaacggt
      721 aaagcgaact agcataataa ctcctgcatg ctttacaaat atatgaaatt taaatcaaaa
      781 agttgaatat gaagagaaaa aatctccata aatgattgtt tttaccgttt tttataagag
      841 aaacatctgt tttttgctaa atcaagctaa ttactctccg aaatccaatt tgggtaatcc
      901 atttactatt tccatggttt tgaggcttag gttgatgaca gagacgaggg tgtcgaggat
      961 gtagcggggg ttttggtgct cgagagccca gtcgttgcaa tcgttggtga ttaggctgtc
     1021 tttgtcctgc gtgacggcgt agcgttcgag gatccagtcg atggcggatt tgccattgac
     1081 gatgtattcg taagcacgct cggggatgtt ttcgattgta atggaattgt tgtagaatat
     1141 ttttgttttg tctgccttgc ttgcaaagcg cattttgtcg gggatgcgga aatacttgta
     1201 gtcttcaggg gtataggttt ggcctgttgt ttttgtaagt ttattgcctg tgatgtttat
     1261 gccgagtgtt ttggctattt tttctgcttc gtcacttgat ttttcgtagt tcaagtgaag
     1321 gtttgcgagg gcgcggcctg cacgctcaaa ggcacagaaa tcttcgtatt tagaaacaaa
     1381 gatgattttg ggtagagact ttttgagatc atctttgaaa gtttctcggt agtctttgct
     1441 gtgaaggaat ccatagacgt aatagaagat gttttctttg gttatttttt tggatgagcc
     1501 ataaaggcgt ttggcttcgg cgagggccca gttagagata ccgtctcttt tttcgagctc
     1561 ttgcgagaac aagtcgcttt tgctttgctc cgcatagtag tagagaggga agcattgacc
     1621 gttgaaaaca acttgcaaat caggaatgct atctgtaatg atgcaagaaa aatctttgtc
     1681 gccaataccc ttaacacaaa tgacaaggtt ttgcgtgtcg ggggtgggga aaagtttagg
     1741 atttatacct ggtctttcta tccaatcttt ttcaaaataa aggaattgct tttcgtaagg
     1801 acggtaacaa gtttgtctaa tgttttttat gaaattaaat ggaacatccg attcaagact
     1861 tttctttaaa ttgactgtcc agctaatttt tgttgggtca ttgctaataa tatcttctag
     1921 ttttttccct gacttgagaa attttttata aagctctcgt tgtgaattat agaactctat
     1981 gcaagatttc atgtttttgg caagcttctt ttcagagaaa gaataaaccc aagaatctct
     2041 atttgtagcc atgcctatag cgtttgttac aaacaaac
//
LOCUS       DAOOBB010000254         2069 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_59381_length_2069_cov_2.808342, whole genome shotgun sequence.
ACCESSION   DAOOBB010000254 DAOOBB010000000
VERSION     DAOOBB010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59381_length_2069_cov_2.808342"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>917)
                     /locus_tag="PLT31_08960"
     CDS             complement(<1..>917)
                     /locus_tag="PLT31_08960"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPW95300.1"
                     /translation="ADNKTAEIAKWDKKKLEEELKQINWELLGINMTDVGFDDIFASE
                     FKEVVDDDFDEGQYLSDEPYSKQGDVYLLDENRVMCGDSTNAEQVKILMDDKLADMVF
                     TDPPYNVAIGKRGQQYKERGGYGCGMNERTILNDDMGNEEFHAFLLQVFKNLFDSVKE
                     GAPTYVCHADSEGINFRVAFSEAGFKLAQCIIWIKNSFTMGRQDYQWQHEPILYGWKP
                     GAGHYFVDDRTQSTTWFYDKPRKNDLHPTMKPLELVGQAINNSSLVGQLVLDLFGGSG
                     STLIASFKSKRNCYSMELDEKYADVIVKR"
     assembly_gap    918..927
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<928..1151)
                     /locus_tag="PLT31_08965"
     CDS             complement(<928..1151)
                     /locus_tag="PLT31_08965"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HPW95301.1"
                     /translation="MEVKMISVNDIKPYENNPRKNDDAVDEVAASIKAFGFKNPIIVD
                     KNMIIIAGHTRLKAALKLDLTMVPVIIAED"
     gene            complement(1123..1608)
                     /locus_tag="PLT31_08970"
     CDS             complement(1123..1608)
                     /locus_tag="PLT31_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95302.1"
                     /translation="MSDVYQQWNEKGILRTKLLSISFLVRAGITLKEICKKEGITLKD
                     LASMKEKYEDLADACDKKNINGLLFCIDNLVQMAEGYQMKREGREGYKSKNGQDKFKM
                     IDVKIPVPKSLQANAYLLEKCYGKQWALDYERLALAEKKLENGETWEDELDGSENDIG
                     K"
BASE COUNT          566 a          432 c          334 g          727 t
ORIGIN      
        1 aacgcttaac aatcacgtct gcgtatttct catcaagttc cattgaataa cagtttcgtt
       61 tactcttaaa agaagcgatc aaagtgctac cgctaccacc aaataggtct aaaactagtt
      121 gacctacaag tgatgaatta ttaattgctt gaccaacgag ctctaatggc ttcatggttg
      181 gatggagatc attctttcgt ggcttatcat aaaaccatgt cgttgattga gttctatcgt
      241 cgacaaagta atgacctgct cctggtttcc atccatagag aattggttca tgttgccatt
      301 ggtaatcttg tctacccatg gtgaatgaat tcttaatcca gatgatgcat tgagcaagtt
      361 tgaatccagc ttcactaaag gcaactctaa aattaatacc ttcgctatct gcatggcaaa
      421 catatgtagg tgcaccttcc ttaacagaat caaataagtt cttaaaaact tgtaataaga
      481 acgcatggaa ttcttcgttt cccatatcat cattgaggat tgtgcgttca ttcatgccac
      541 agccatatcc acctctttct ttatattgct gccctctttt cccgatggca acgttataag
      601 gtggatcggt gaaaaccata tcagcaagct tatcatccat aaggatttta acttgttctg
      661 cattggttga atcaccacac atgacacgat tttcatctaa gagataaaca tctccctgtt
      721 tagaataagg ttcatcagat aggtattgac cttcatcgaa atcgtcatct actacttctt
      781 tgaattcact agcgaagata tcatcaaacc caacatcggt catattaatt cctaatagtt
      841 cccagttgat ttgtttgagt tcttcttcta gtttcttttt atcccacttt gcgatttcag
      901 cggttttatt atcagcannn nnnnnnnaaa tcttcggcaa tgattactgg aaccattgtt
      961 aagtctaact tcaaagcggc ttttaatctg gtgtgaccag caatgatgat catgtttttg
     1021 tcaacgatga ttggattctt gaagccaaag gccttgatag aagccgctac ctcatcaacc
     1081 gcatcatcat tcttacgcgg gttgttttca taaggtttaa tatcatttac cgatatcatt
     1141 ttcacttcca tctaattcgt cctcccaagt ttcaccgttt tctaatttct tttcagctaa
     1201 agctagtctt tcataatcaa gagcccactg ctttccatag cacttctcta agagataagc
     1261 attagcttgt aatgatttag ggacagggat tttgacatcg atcatcttga atttgtcttg
     1321 gccgttcttt gatttatatc cttcacgacc ttctcttttc atttgataac cttccgccat
     1381 ttggactaag ttatcgatgc agaaaagtaa tccattgatg ttctttttat cgcacgcatc
     1441 tgctaaatcc tcatattttt ctttcataga ggcgagatct tttaatgtga ttccttcttt
     1501 tttgcagatt tctttaagtg tgatacctgc tcttactaaa aacgagattg atagaagctt
     1561 tgttctaaga atccctttct cattccattg ttgatagacg tctgacattt ttctttcctc
     1621 ctttttgcca gtgctacaga tttctgcagg aagcaaaaaa gagccagacg cggctcttaa
     1681 taagaatcct aggctttcgc cagcttattc gtgtagacca cttgcctaca cttatcgatc
     1741 ataatcatag tttaccgaaa aacagcattt tcctcaattg acgcgaagta acattatgaa
     1801 gggcttttaa tcttagatta agtgaaattc gagtgtagaa aaatgatgaa attttgcatg
     1861 ttttttacac ttttttgttt ttgaacctga ccgataattt ttagcggtta ggtctccatc
     1921 acaacctagc cactaccgag gttatgaaaa agactcacca ctttgtgagc cttattcgtt
     1981 ttttccttaa tactatgtat tagtcatttt ctatgacaca taacaaggat tatttctctt
     2041 tacaattggg tatatatttt tctttcaat
//
LOCUS       DAOOBB010000255         2051 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_60071_length_2051_cov_4.371242, whole genome shotgun sequence.
ACCESSION   DAOOBB010000255 DAOOBB010000000
VERSION     DAOOBB010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60071_length_2051_cov_4.371242"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="PLT31_08975"
     CDS             complement(<1..522)
                     /locus_tag="PLT31_08975"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HPW95303.1"
                     /translation="MEEVVVTGMGCVSPLGNTPEELWKNLLAGKSGVSNIERFNTDEY
                     DVHFGGEVKVFDPTPYFSERDLKRFPRNVQYAMHCAMKAVEQAGLTDLIDKSRAGVIL
                     ASGMGGVDVYYKGSIDLATKGPRHLSPYFMPMVISNMASNLVTIRLGWMGPSFATLSA
                     CASSNYSLISAVDQ"
     gene            complement(689..1921)
                     /gene="fabF"
                     /locus_tag="PLT31_08980"
     CDS             complement(689..1921)
                     /gene="fabF"
                     /locus_tag="PLT31_08980"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HPW95304.1"
                     /translation="MEEVVVTGMGCVTPLGNNPNELWTNLLAEKSGISNIIRFNTEAY
                     DVHFAGEIKNFDPSPYFNVRDAKRYSRNIQYVVHSAMTALENAGLDPKSVDPTRAGVI
                     LASGMGGMDVYYETSVTLSTRGPGRVSPFFIPMVISNMGSGEVSIRLGWMGPSFTPVS
                     ACASSNHSIISAVDQIRLGRADVMIAGGTEEAVCPIGIAGFAAMRAISTRNDDPERAS
                     RPFDKDRDGFVLGEGAGVLILESLSHAKKRGAKILARIAGVGMSADAHHISAPREDGL
                     GVKLAIEMALNDARITSKDVGYVNTHGTSTPLGDVAECGAIYKAFDGHVDHLKVNSTK
                     SMTGHMLGAASAVEGIVTVQSILDQKVHATKNVFEKDSRILLDVCDKGASNHNIKYAM
                     SNSFGFGGHNSVVVFAKD"
BASE COUNT          695 a          446 c          334 g          576 t
ORIGIN      
        1 ttgatcgaca gcagaaataa gagaataatt tgaagaagca cacgccgata atgtagcgaa
       61 agatggcccc atccatccta aacgaatggt caccaaatta gaggccatat tagaaatgac
      121 catcggcata aaataaggcg ataaatgacg aggtcctttt gtcgctaaat ctatagatcc
      181 cttatagtat acatctactc cacccatgcc acttgctaaa atcacaccag ctctcgactt
      241 atcaattaaa tccgttaagc cagcttgctc gacggctttc atagcacaat gcatagcata
      301 ttggacgttt cttgggaatc tttttaaatc tcgttctgaa aaatagggcg tgggatcaaa
      361 aactttgact tcaccgccaa aatgaacatc gtattcatct gtattgaatc gttcaatgtt
      421 agatacccca gacttacctg caagaagatt cttccagagc tcttcaggag tatttccaag
      481 tgggctaaca caacccatac cagttacgac tacttcttcc atcaaatcac ctgtctatat
      541 gttttttctt cagctaaaag tttatctcta tgtgttcaat aatactcttt ttcaatactt
      601 atcaaattct tgcgattttt gcactaaaaa agagttaaaa catctctaga ctcatgctaa
      661 aagaatgagt ctagagattt catacaaatt aatctttagc aaaaacgacc acactattgt
      721 gaccgccaaa gccaaagcta ttagacatgg catattttat gttatgatta gaagcccctt
      781 tatcacatac gtctaaaagg attctggaat ctttttcaaa cacattttta gtcgcatgca
      841 ctttttgatc aagaatagac tgaaccgtaa caataccttc cactgcagat gcagctccaa
      901 gcatatggcc cgtcatagat tttgtggaat tcactttcaa atgatcaacg tgaccatcaa
      961 aagctttata aatagcacca cattcagcga catcaccaag aggagtggat gtgccatgag
     1021 tattcacata accaacatct ttagatgtaa tacgagcatc atttaaagcc atttcaatag
     1081 ctagttttac acctaaaccg tcttcacgag gtgcagaaat atgatgagca tcagcactca
     1141 tgccaacacc agcaatacga gcaagaattt tagcgccacg tttttttgca tgagaaaggc
     1201 tttcaagaat taaaacacca gcaccttcac ctaatacaaa accgtcacgg tccttatcaa
     1261 atggacgaga agctctctca ggatcatcat tacgagtact aattgcacgc atcgctgcaa
     1321 aaccagcaat accaattggg caaacggctt cttcagtacc gccagcgatc atcacatcag
     1381 cacgacctaa acgaatttga tcgactgcag aaataatgga atgattagaa gaagcacaag
     1441 cagaaacagg agtaaaactt ggccccatcc agcctaaacg gatagagact tcaccagatc
     1501 ccatattaga aatcaccatt ggaataaaga atggagaaac acgacctgga ccacgagtgg
     1561 ataatgtaac agaagtttca taataaacat ccataccacc cataccactt gcaagaataa
     1621 caccagcacg agttgggtca acggatttag gatctaagcc agcattttct aaagctgtca
     1681 ttgcagaatg caccacatac tgaatgttac gagaataacg ttttgcatca cgaacattga
     1741 aataaggcga tggatcgaaa tttttaattt cacctgcaaa atggacatca taagcttctg
     1801 tgttgaaacg tatgatgttt gaaataccgg acttttcagc caataaattt gtccataatt
     1861 cgttaggatt attgccaaga ggggttacac aacccattcc tgtcactact acttcttcca
     1921 tggttaactt cctataatta aattgtttgg cgaaaattta taaaaaagac attctttttt
     1981 tcaatatttt atttgattcc gattcattct taataaaaaa aaaatttgaa taaattattt
     2041 aattttcttt t
//
LOCUS       DAOOBB010000256         1990 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_62645_length_1990_cov_4.471318, whole genome shotgun sequence.
ACCESSION   DAOOBB010000256 DAOOBB010000000
VERSION     DAOOBB010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62645_length_1990_cov_4.471318"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PLT31_08985"
     CDS             complement(<1..414)
                     /locus_tag="PLT31_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HPW95305.1"
                     /translation="MNRSWIFLLFFASFLFSSPPVNSYVFDENRLLTTQETETFNLIA
                     QELYNKTGVGLAAALYQDIGEEDPREFALKTAESFGIGKKGVDEGILIFVAMNQKRRS
                     VEVGYGAEPYLPDVLVEKIQQQTLVPAFRQRKYGEG"
     gene            complement(430..1662)
                     /locus_tag="PLT31_08990"
     CDS             complement(430..1662)
                     /locus_tag="PLT31_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95306.1"
                     /translation="MKLFSAITLTLMIAVSASFGAKLISYTGYSAESQEEANNAAIAG
                     VAKQISTQVSTSQKLVKKEIKKGNASSFNKNFSVSNQVESNLLLKGIRVSPKPKQGNS
                     FVAFATVDLDQLSSELRFNIQKIQKDVKQTEIEIIKSIEQKRYADAFEKLTSLRMLVL
                     KHPPLLESLAEFYPIDSSFSLSSIDQELERTLIEKLSNITVSIKDPQEYEITTPILPS
                     FTVYVSDKEGPIPNFPIYVMQNNRTLGERYTKDAGEVSFALKNVNFDQGPYTIQIAVN
                     FPHMILKKTGLDKPLEIQYKTKKTKCSYYLSCKETVNVCNLIENRLATKSFFHNPNNQ
                     KNTIQAKVTSEIQKAMKAGRNNIISYSVSLQLTGPEINFLKKEKVIAKSEYEAVIKFL
                     EKVDLNDFSTQASFLCKE"
     gene            complement(1664..>1990)
                     /locus_tag="PLT31_08995"
     CDS             complement(1664..>1990)
                     /locus_tag="PLT31_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95307.1"
                     /translation="LEKKISAYWSADQKNGVQYKAVIKIKGSYSNTQIEDVHESVLST
                     LKTIFNKVSVNAMTSKTMDLIIYADPQKYADSHEVYSAIRNSLKSVASISKTNISRKL
                     LLMDLQ"
BASE COUNT          599 a          319 c          375 g          697 t
ORIGIN      
        1 gccttcgcca tacttgcgtt gcctaaatgc tggaactaat gtttgctgtt gtatcttttc
       61 tactaaaaca tcgggtaaat agggctctgc tccgtaaccg acttctacac tgcggcgttt
      121 ttgattcatg gctacaaaaa taagaatgcc ttcatcaacg ccctttttcc ctattccaaa
      181 agattcagct gttttgagcg caaattctcg ggggtcttct tctccaatat cttggtataa
      241 agcggcagca aggcctacgc ctgttttatt gtaaagttct tgagctatta aattaaaagt
      301 ctctgtttct tgcgttgtga gcaagcgatt ttcatcaaat acataagaat tgacaggagg
      361 gctagaaaat aaaaaggaag caaaaaagag taagaaaatc caagaacgat tcatgacttc
      421 aagatagcat tattctttac ataaaaaact agcttgagtc gaaaaatcat tcaaatctac
      481 tttttctaaa aacttaataa cggcttcgta ttctgatttg gcgatgactt tttctttttt
      541 cagaaaatta atctcaggcc cagtaagttg aagcgaaaca gagtatgaaa taatattatt
      601 tctacctgct ttcattgctt tttgaatttc agaagtcacc ttggcttgaa tggtgttctt
      661 ttggttgttt ggattatgga agaaagactt tgtggcgaga cgattttcaa ttaaattgca
      721 cacattcaca gtctctttac aagataagta ataagaacat tttgtctttt ttgttttata
      781 ctggatttca agaggtttat ctaaaccagt cttttttaag atcatgtgag gaaagttgac
      841 tgcaatttgg attgtatacg gcccttggtc aaaattgaca tttttgaggg caaaagaaac
      901 ttctccagcg tcttttgtat agcgttctcc aagcgttcta ttattttgca ttacataaat
      961 aggaaagttt ggaataggac cttctttgtc cgatacgtat acagtaaagg aaggtaaaat
     1021 aggagtggta atctcgtact cttgtggatc tttaatagat acggtaatgt tcgaaagttt
     1081 ttctataaga gtccgttcta attcttgatc gatactggat aaagaaaaag aactatcgat
     1141 agggtaaaat tcagcaagag attctaataa aggagggtgt tttagaacta gcattcttaa
     1201 agaggtgagt ttttcaaagg catcggcata tcgcttttgt tcaatggatt tgataatctc
     1261 aatttccgtt tgtttaacgt ctttttgaat cttttgaatg ttaaacctta attcagaaga
     1321 aagctggtct aaatcgactg tggcaaaagc cacaaaacta ttaccttgtt taggcttagg
     1381 agaaacacga atccctttta ataagagatt tgattctact tggtttgaaa cagaaaaatt
     1441 tttattgaaa gaagaagcgt tgcctttttt tatttctttc ttgacgagct tttgagaagt
     1501 agaaacttgt gttgaaattt gtttggcaac gcctgcaatg gctgcgttat tcgcttcttc
     1561 ttgagattct gccgaatacc ctgtatagct gatcagtttt gctccaaaag aagcagaaac
     1621 agctatcatt aaggttaagg taatggcaga aaataatttc attttattgt aaatccatta
     1681 aaagaagttt tctagaaata ttagtctttg aaatagaagc tacacttttt aggctatttc
     1741 taatggcact atagacttca tgagaatctg cgtatttttg tgggtcagcg taaataataa
     1801 gatccattgt tttagatgtc attgcattta cagagacttt attaaaaatg gtttttagtg
     1861 tgcttaagac agattcatgt acatcttcaa tttgggtatt ggaatacgag cctttaattt
     1921 ttatcacggc tttgtactga accccatttt tttgatctgc actccagtag gctgaaattt
     1981 tcttttctag
//
LOCUS       DAOOBB010000257         1972 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_63433_length_1972_cov_4.365676, whole genome shotgun sequence.
ACCESSION   DAOOBB010000257 DAOOBB010000000
VERSION     DAOOBB010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63433_length_1972_cov_4.365676"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PLT31_09000"
     CDS             complement(<1..285)
                     /locus_tag="PLT31_09000"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPW95308.1"
                     /translation="MILKINEHIELKSIQLRDAPEIFNVIDSERETLRRWLPFIDATL
                     TVADTENYIQSIESMPVNSRDLVFLIRYQGDFAGLIGLKSIDFNNKRAEIG"
     gene            complement(354..575)
                     /locus_tag="PLT31_09005"
     CDS             complement(354..575)
                     /locus_tag="PLT31_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95309.1"
                     /translation="MSENEKKISAAEAIEISLREKRVVKIRDVDGEQELALNQICDNC
                     CSAEATVDFWGVREGDEWHVCVIKETSDL"
     gene            742..1509
                     /locus_tag="PLT31_09010"
     CDS             742..1509
                     /locus_tag="PLT31_09010"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPW95310.1"
                     /translation="MMLKGKIAIVTGASSGMGNEIAFKYAMEGAKVIAVARRLEKLEH
                     LAEKAKKHGGEITPLVADISKQKEAESLVIEAIKRFDKLDILVNNAGIMDDFSPVGDV
                     SDEMWTKVMNVNINGPFYTTRSAVKYFLTREEGGIIINVASIGGLFGARAGAAYTASK
                     HALVGLTKNTGFMYSKHNIRCNAICPGGIVTDIGSGEFMKSVNQQGLAMASAGIGANP
                     RMGLGSEIASVAVMLATEASSYINGQCIAVDGGFTAY"
     gene            1638..>1972
                     /locus_tag="PLT31_09015"
     CDS             1638..>1972
                     /locus_tag="PLT31_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95311.1"
                     /translation="MNEKKRFAINAFAQIFSFTVSLGITFFLTPYIVGHLGTAAYGFV
                     GLAQNFVSYAQLITIALNSMAGRFISISYHQGNIEQAKKYFTSVFYSNAILLSFVALC
                     SCFVIYRLE"
BASE COUNT          611 a          383 c          350 g          628 t
ORIGIN      
        1 acctatttcg gcccttttat tgttaaaatc tatgcttttt aggccaatta acccagcaaa
       61 atcaccctga taacgaatca aaaagaccaa atctcttgaa tttacgggca tagattcaat
      121 cgattgtata taattttctg tatcagcgac agttagcgta gcatcaataa aaggaagcca
      181 tcgacgcaaa gtttctcgct cactatcaat cacattaaaa atttctggag catctcttaa
      241 ctgaattgat tttaactcaa tgtgttcatt tattttaaga atcattctta taaatataag
      301 ctctaacctt ataagaataa gatttttttg aaaaagaatg gcacaaagaa agactaaaga
      361 tcacttgttt ctttgataac acaaacatgc cattcatcgc cttcacgaac gccccaaaaa
      421 tcgaccgtag cctcggcaga gcaacaatta tcacagattt gatttaaggc aagctcttgt
      481 tctccatcca catctcttat cttaaccaca cgcttttcac gaagactgat ttcaatggct
      541 tcggcagcac ttattttttt ttcattttca gacatatttt acacctcttt tatttatcga
      601 catgaaatca aagtatcatt atcgtttaga ataaatcaag ccagaacctt tttttaataa
      661 aaaaacatcc ctaaaaaaaa gaattaacac atttaaaaca tatttatgta tgttaattat
      721 aagaaatcta cggaggatat catgatgtta aaaggtaaaa tcgccattgt cactggcgca
      781 agttctggaa tgggcaatga gattgcattc aaatatgcaa tggagggggc taaagtcatt
      841 gctgtggcta ggcgtcttga aaaattagaa caccttgccg aaaaagcaaa aaaacatgga
      901 ggagaaatca cccctctcgt ggcggatatt tcaaaacaaa aagaagccga gagccttgta
      961 attgaagcta tcaaacgttt tgataaacta gacatcctag tcaataatgc aggtattatg
     1021 gacgacttca gccctgtagg cgatgtgagt gacgaaatgt ggacaaaagt catgaacgta
     1081 aacatcaatg gcccctttta cactactcga agcgctgtta aatatttctt aactcgtgaa
     1141 gaagggggca tcattattaa tgtagcctct attgggggtt tatttggagc aagagcaggc
     1201 gcggcttaca ccgcctccaa acacgctcta gttggcctta ccaagaacac tggtttcatg
     1261 tattctaagc acaatattcg ctgtaacgcc atttgtcctg gtggcattgt tactgatatt
     1321 ggctctggag aattcatgaa atccgtaaat caacaaggtt tggccatggc gtctgcaggc
     1381 ataggtgcta acccaagaat gggtctagga tcagaaatcg cctctgttgc tgttatgctt
     1441 gccacagaag cctcctctta tatcaacgga caatgcattg ccgtagacgg gggctttacc
     1501 gcctattaaa aacattcatt ttgccttaaa gagacctact attaggtctc ttttttctat
     1561 tttggccttt atgaatacag ttcttatttt tgctggtggt tctagcatac gaatgaatgt
     1621 gagaaataaa aaaagtcatg aacgaaaaaa aacgatttgc cattaatgca tttgctcaga
     1681 ttttttcgtt tactgtgagc ttaggcatta cctttttttt aactccttat attgttggtc
     1741 atttgggaac cgccgcttat ggatttgttg gactcgctca aaattttgta agttatgctc
     1801 agcttattac aattgcctta aactctatgg cgggtcgttt tatatccatt agttatcatc
     1861 aagggaatat tgagcaagcc aaaaaatact ttacttctgt tttttattct aatgcaattc
     1921 ttctctcctt tgttgcatta tgttcttgtt ttgtaattta tcgattagag ca
//
LOCUS       DAOOBB010000258         1925 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_65587_length_1925_cov_2.876471, whole genome shotgun sequence.
ACCESSION   DAOOBB010000258 DAOOBB010000000
VERSION     DAOOBB010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65587_length_1925_cov_2.876471"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PLT31_09020"
     CDS             <1..346
                     /locus_tag="PLT31_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014544876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HPW95312.1"
                     /translation="DEISHQVSEGDGPVNALDAALRKALLPYFPYMKKVRLDDFKVRV
                     LGSNVGSDATVRVWTTFGDEKEQWNVAGVSANIIEASWLALVDGLCYKIIKEAKQPKS
                     TLKRAKRVSKKG"
     gene            351..1622
                     /gene="hisD"
                     /locus_tag="PLT31_09025"
     CDS             351..1622
                     /gene="hisD"
                     /locus_tag="PLT31_09025"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014544877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HPW95313.1"
                     /translation="MKIVKVKPNSAEIKRLCSRAVAPTKEIQAKVDQILLDVRTHGLP
                     KVIEYAKAYDGLKGNRLRVSESVIEKAAQKTPPALQKALKQAIRNVKVFHQNQVEESW
                     QITGKDGVVLGQRIRPMRRVGLYVPGGSGTYPSTVIMCAVPAIVAGVSEIVVVTPIKD
                     GLQPSLAFVLRELGITEVYHVGGAQAIGLLAYGAKGIEPVDKIVGPSNVFAAIAKKEV
                     FGVVDIDMIAGPSEILVLADATCDPDFVAADLLSQAEHGSGFEAAICITDNMETAQMI
                     SACADIQVEGSPKRELLEKALDQYGRILVVDNWFDGIEIANKIAPEHLEIMTSEAKKM
                     SESIENAGAIFIGPYSSEPVGDYFAGPDHVLPTNGTARFSSPLGVYSFLKRTSIIEYS
                     SAAMSKNGKAIASIASAEGFFHHAQAILKRL"
     gene            1665..1738
                     /locus_tag="PLT31_09030"
     tRNA            1665..1738
                     /locus_tag="PLT31_09030"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1699..1701,aa:Pro,seq:tgg)
BASE COUNT          575 a          314 c          424 g          612 t
ORIGIN      
        1 agatgaaatt agtcatcaag tttctgaagg tgatggacct gtgaatgcct tggatgcggc
       61 acttcgcaaa gctcttctac catattttcc ttatatgaaa aaggtgcgtt tagatgactt
      121 taaggtacgc gttttaggct caaacgttgg ctcagatgct actgttcgag tttggaccac
      181 ttttggtgat gaaaaagaac agtggaacgt cgctggagtc agtgctaata ttatagaagc
      241 ttcttggtta gctttagtgg atggcctttg ttataaaata attaaagaag caaaacagcc
      301 aaaatccaca ttaaaaagag caaaacgtgt ttctaaaaaa ggttgatttt atgaagatag
      361 tgaaagtaaa acctaattca gctgaaatta agcgcttatg ttctcgagca gttgcgccca
      421 ctaaagaaat tcaagcgaaa gtagatcaaa tccttctgga tgttcgtact catgggcttc
      481 caaaggtaat tgaatatgcc aaagcatacg atggcttaaa gggaaatcga ttaagagttt
      541 ctgaaagtgt aattgaaaaa gcggctcaaa aaacacctcc agctttacaa aaggccttaa
      601 aacaagcgat tcgaaatgta aaagttttcc atcaaaatca agtagaagaa tcatggcaga
      661 ttacaggtaa agatggagtc gttttaggcc aacgtattcg ccccatgcgt cgtgttgggc
      721 tttatgttcc aggtggttcg ggtacttatc ctagtacggt gattatgtgt gctgttcctg
      781 caatagttgc tggagtttct gaaattgtgg tggtcactcc aattaaagat ggcttacaac
      841 cttctctagc ctttgttttg cgtgaacttg gtattactga agtgtatcac gttgggggag
      901 ctcaagcgat agggctttta gcttacgggg ctaaaggcat tgaacctgta gataaaattg
      961 tcggccctag taacgtcttt gcggctattg ccaaaaaaga agtttttggt gttgtggata
     1021 tcgatatgat tgcaggtcca tcggaaatac tggttctagc ggatgccaca tgcgatcctg
     1081 attttgtcgc tgcagatttg ctttctcaag cagagcatgg ctctggtttt gaagccgcta
     1141 tttgtattac cgataatatg gaaacggctc aaatgatttc tgcttgtgct gatattcagg
     1201 tggaaggttc ccctaaaagg gaacttttag agaaagcttt agatcaatat gggcgtattt
     1261 tagtggtaga taattggttt gatggcattg aaatcgctaa taaaattgct cctgaacact
     1321 tagaaatcat gacttctgaa gcaaaaaaga tgtctgaatc catagaaaat gcgggcgcta
     1381 tatttatagg gccatattct agtgaacctg ttggcgatta ttttgctggt cctgatcatg
     1441 ttttgccaac aaatggaacg gcacgttttt caagtccact tggagtatat tctttcttaa
     1501 aaagaacaag tattattgag tattcttcgg cggcaatgtc aaaaaatggt aaggcaattg
     1561 cctctattgc ctctgcagaa ggattttttc atcatgctca agcaatttta aagcgattat
     1621 aatcttgttt tctaaaaaat ctttgttata tttgtcttcg atttcgggat gtagctcagt
     1681 ctggtagagc gtctgctttg ggagcagaat gtcgtcagtt cgaatctggc tatcccgata
     1741 aaaaaacctg ataataaatt atcaggtttt tttgtttaaa ataccaatga atgttttcaa
     1801 tatactagat acttctagta taaatcggcg tatttaagtt gtatgtaaaa taaaaaaaag
     1861 gagaaataat tttttttaat tctcctttgt aatcaacggt gtagtttaga agagaatact
     1921 atttt
//
LOCUS       DAOOBB010000259         1916 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_65955_length_1916_cov_4.856529, whole genome shotgun sequence.
ACCESSION   DAOOBB010000259 DAOOBB010000000
VERSION     DAOOBB010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65955_length_1916_cov_4.856529"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1683)
                     /locus_tag="PLT31_09035"
     CDS             complement(<1..1683)
                     /locus_tag="PLT31_09035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02171 family protein"
                     /protein_id="HPW95314.1"
                     /translation="MRLVFLLFTILFTSCALDDGKPLGVLFEYDQEHEGMIRIKSEGN
                     RVVLGTSESTAKFSESPKMNVDFTYSFSISKNEVTRNEYAEFMGGESSSIQDSGDLPQ
                     TNVTYYDAVLYANARSVSEGYDTAYTYSSVIYDAKGNCTYLDSLLFNPSKEAYRLPTE
                     AEWVFAASHNWRPNQGWNNLNSDYRVHKVCTADINALSICDMAGNAMEWVNDHLGNFK
                     DTTLINYIGASKENSFKERIVKGGSYHKSPSQAQLYSRGDVYTVTSSTKAEYVGFRLV
                     FGRIPDPLYFDSKNEISNQFNIVASLSSIKEMTESNHLKMAFVNKGSSSLVYADFSKM
                     GLSLYEIKDNLKVNHPDISPDGNYVAFCTGLEGVSGSSQVYVRDLNPSGSNLVRLNVE
                     SAAIPRFRVLPSGDTVIVYVSDAGNNKEESEWKQKSTWQVPFKNGKFGTPEKLFDGSY
                     HGGFSEDGSLAVTGARLLRANVNGKDSIWYNGEQACNVSLSKDNKKQTLFLDFVGKTG
                     KDFAGKSYAVHEYLWLADSTGKIDKYIAAPKGKTWDHTEWSSIEDVAVASVVN"
     gene            complement(1685..>1916)
                     /locus_tag="PLT31_09040"
     CDS             complement(1685..>1916)
                     /locus_tag="PLT31_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95315.1"
                     /translation="ETGAFGRYGLKRSRAKMLIEEMNAFEDKYLNFYLMDENKMGDHD
                     YTEEMAFDYDHLSVLGAVQLTSRLDSLLQTLE"
BASE COUNT          586 a          411 c          294 g          625 t
ORIGIN      
        1 atttactaca gaggccacag cgacgtcttc tatactagac cattctgtat gatcccaagt
       61 ttttcctttt ggtgcggcaa tgtatttatc tatttttcct gtgctatctg caagccataa
      121 gtattcatgg actgcgtatg attttccagc aaaatctttt cccgtcttac ctacaaaatc
      181 taaaaataga gtttgctttt tattgtcttt agaaagggat acgttacaag cttgttctcc
      241 attataccaa atcgaatctt ttccattgac gttcgcgcga agtaatctcg ctcctgtgac
      301 agctaaagag ccatcttctg aaaaaccacc atgataactt ccatcaaaaa gtttctctgg
      361 agtaccaaat ttaccgtttt taaaaggaac ctgccatgta cttttctgtt tccattcgct
      421 ttcttcttta ttattgccag catcacttac gtaaacaatg acagtgtcac cactaggtag
      481 cactcgaaaa cgaggaatcg ctgcactttc tacatttaaa cgaactaggt tagatccaga
      541 gggattaaga tctcgtacat acacttgtga agatcctgaa acgccttcta agcctgtaca
      601 aaaagccaca taattcccat caggagaaat atctgggtga tttaccttta aattatcttt
      661 gatctcatac aatgataatc ccatttttga aaaatctgca tagacaagag agcttgaacc
      721 cttattgaca aacgccattt ttagatgatt ggattctgtc atctctttaa tgctacttaa
      781 agaggccaca atattgaatt gatttgaaat ttcattttta gagtcaaagt ataatggatc
      841 tggaatacgg ccaaatacta aacggaaacc cacatattca gcttttgtag aggacgtcac
      901 tgtataaaca tcgcctcttg aataaagttg agcctgacta ggagatttgt ggtaactccc
      961 cccttttacg attcgctctt taaatgaatt ttctttggat gctcctatat aattaattaa
     1021 agtcgtatcc ttaaaattgc ctaaatgatc attcacccat tccatggcat tccctgccat
     1081 atcacaaatg gataacgcat tgatatctgc tgtacaaacc ttatgcacac gataatccga
     1141 atttaaatta ttccatcctt gatttggcct ccaattatga gaagcggcaa agacccattc
     1201 cgcttctgta gggagtcgat acgcttcttt tgatggatta aataaaagac tatctaaata
     1261 agtacaattg cctttggcat catatattac tgaagaatat gtataagccg tatcataacc
     1321 ttcgctcact gaccttgcat tagcataaag aacggcgtca taataagtca catttgtctg
     1381 tggaaggtct ccgctatctt gaatggaact cgattctcct cccataaatt ctgcatattc
     1441 atttcgagtc acttcatttt tactgataga gaaagaataa gtgaaatcca cattcatttt
     1501 tggactttct gaaaatttgg cggtcgactc acttgttcct aatacgactc tgtttccttc
     1561 ggattttatc ctaatcatgc cttcatgctc ttgatcatat tcaaaaagaa cacccaatgg
     1621 ctttccatca tcaagcgcac aagaggtgaa taagatcgta aataacaaaa atactaatcg
     1681 catattattc cagcgtttgt aaaagtgaat ctaaacgtga cgtaagttga acagcaccta
     1741 atacacttaa atgatcataa tcgaaagcca tttcttcagt ataatcatga tcacccattt
     1801 tattttcatc cattagatag aaatttagat atttatcttc aaaagcattc atctcttcta
     1861 ttaacatctt ggcacgactc ctttttaatc cgtatcgacc aaaagctcca gtttct
//
LOCUS       DAOOBB010000260         1867 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_68255_length_1867_cov_3.705298, whole genome shotgun sequence.
ACCESSION   DAOOBB010000260 DAOOBB010000000
VERSION     DAOOBB010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68255_length_1867_cov_3.705298"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="PLT31_09045"
     CDS             <1..550
                     /locus_tag="PLT31_09045"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HPW95316.1"
                     /translation="SYYDDPFDSKGFERNVLYHYPFMNDKLIIGKFCAIARDVKFMMN
                     GANHKIDAFSSYPFSIFRNGWERVTPTLEDLPFKGDTIIGNDVWIGYDALIMPGVKIG
                     DGAIIASKSVVVKDVPAYTIVGGNPANEIRRRFSEDVIALLESAQWWNWSAQKITDSL
                     EVLTSNDEDALRSLFYTDRDFS"
     gene            complement(578..1519)
                     /locus_tag="PLT31_09050"
     CDS             complement(578..1519)
                     /locus_tag="PLT31_09050"
                     /inference="COORDINATES: protein motif:HMM:NF015558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HPW95317.1"
                     /translation="MKEKLSKIFFILIILIACFPQISSWMALLAGITYAFIFGGPAYP
                     NFAKKTQKYLLQGCVVGLGFGMNLHQALASGKEGMLFTVFSVVIVMVLGFIVGKVIKV
                     DTKTSYLISSGTAICGGSAIAAVSPVVKADDKQMSVSLGTIFLLNALALLIFPPLGHF
                     FDLSNQQFGEWAAIAIHDTSSVVGAAAAYSDESLQVAALVKCTRALWILPLALVTMLF
                     FRKNSSSGRLNVIPWFIILFALAMVINTYVFPQFGISESISEYIVFAAKRGFAITLFL
                     IGTNLSWKALKSCGSRPFIQGVILWAAIAIISLLVIL"
BASE COUNT          605 a          298 c          385 g          579 t
ORIGIN      
        1 ttcttattat gatgatccat ttgattcgaa aggttttgaa cgaaatgttc tttatcatta
       61 cccttttatg aatgataaac taattattgg gaagttttgt gctattgctc gtgatgtgaa
      121 atttatgatg aatggggcaa atcataaaat agatgctttt tcatcttatc cattttccat
      181 ttttaggaat ggttgggaac gagtgactcc tacgcttgaa gatttgcctt tcaaagggga
      241 tacgattatc ggtaatgatg tttggattgg ctatgatgct ttgattatgc ctggggtaaa
      301 aattggagat ggagcgatta ttgcctctaa atcagttgtt gtaaaagacg tacccgcata
      361 caccattgtc ggtggtaatc ctgcaaatga aatcaggaga cgctttagcg aagatgttat
      421 tgctcttctt gaatcggctc aatggtggaa ttggtcagct caaaaaataa cggattctct
      481 agaagtgtta acctcaaatg atgaggatgc tcttcgcagt ctgttttata cagatagaga
      541 tttttcttaa aaaactgttg ttttttaagt ccttgattta gagaatcact agtaaactga
      601 ttatagcaat tgcagcccat aaaatgactc cctgaataaa aggacgagag ccacaggatt
      661 ttagtgcttt ccaagaaagg tttgtgccta ttaagaataa ggtgatggca aatcctcgtt
      721 tggcggcaaa gacaatgtat tcgcttatgg attctgaaat tccaaattgt gggaatacgt
      781 atgtgttgat gaccatcgct agcgcaaata agatgataaa ccaagggatg acattgagcc
      841 ttccagaact actgtttttc cgaaagaaca acatggtcac tagagcgaga ggcaaaatcc
      901 agagggctcg cgtgcatttc actagtgccg cgacttgtag ggactcatca ctataggcgg
      961 ctgcagctcc aacaacagaa cttgtgtcgt gaatagcgat agcagcccat tctccaaatt
     1021 gctggtttga aagatcaaag aagtggccaa gtggagggaa aataagaaga gctagagcat
     1081 ttaataaaaa tatggtgcca agcgaaacac tcatttgctt atcatcggct ttgactacgg
     1141 gagaaacggc tgcaatggct gaacctccgc aaatggctgt tccagaggat attaaataag
     1201 atgtttttgt atcgacttta attactttac ctacaataaa gcctaaaacc attacaataa
     1261 cgacagaaaa gacggtaaat aacatccctt ctttgccaga ggcaagagcc tgatgcaagt
     1321 tcatgccaaa gccaagccct acaacgcagc cttggagtag atacttttga gtctttttag
     1381 caaaattagg gtaggctggt ccaccaaaaa taaaagcgta agtaatacca gctaaaagag
     1441 ccatccaaga tgaaatttgt gggaaacagg caattagaat aataagaata aagaatattt
     1501 tagatagttt ttctttcatt ttttttaccg tgaatataaa caatgatagt tttttatctt
     1561 ttatttgttt agtcgttagg gatgatcatt ttttgggttt attatgaatt taagcataca
     1621 agattttggc tataatgaaa gagtagatca attaagaatt gaaaacaatc tccaaaacta
     1681 tgctttccaa aattaaaatt tcgaaatatt caccatttta aaaagactac tattcatgga
     1741 aatcgagaac taatgcttta tttgtcatcg gttgttttaa aaatggaaaa ctttatattg
     1801 tatctgaata aagaacagtt tgtattttta tttttttttg acggcaagaa agaagaatgt
     1861 atggggt
//
LOCUS       DAOOBB010000261         1856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_68807_length_1856_cov_4.495280, whole genome shotgun sequence.
ACCESSION   DAOOBB010000261 DAOOBB010000000
VERSION     DAOOBB010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68807_length_1856_cov_4.495280"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PLT31_09055"
     CDS             <1..317
                     /locus_tag="PLT31_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HPW95318.1"
                     /translation="LRDVSFLNSDLKGARFNADTKMENVDFSGADLTGAKIEKFTAKK
                     VIYDAKTKFPSGFEPRQYGFTRHGEKAAEIVIKRDPTVRNSTVTDDAMPKKRKKRKKS
                     AE"
     gene            401..1777
                     /gene="radA"
                     /locus_tag="PLT31_09060"
     CDS             401..1777
                     /gene="radA"
                     /locus_tag="PLT31_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HPW95319.1"
                     /translation="MAKNKTTIEYVCKECSSTFSKWSGKCSACGAWSTLEERIIEDKA
                     NKRGLGGQDLESSSQTLKSIQSQENQRLSTGNSEFDRTLGGGLAPGSLILIGGDPGIG
                     KSTLVLQTLATMSAANVKTLYVSGEESAVQVKIRSERLELSGSDMLLMCETNLEKVLA
                     EAKRIQPEILVIDSIQTVYKTELPGTPGSVTQLRECTLELMVYAKNTGCITILVGHVT
                     KDGQIAGPRILEHMVDTVAYFEGDRNHQYRVLRTIKNRFGATDEIGVFEMTSRGLEAV
                     ENPSKVFLQNSEEPLPGSVISCTLEGTRALLFETQALVSQTNYAVPQRVAAGIDPKRL
                     TIILALLEKFASIRIGSSDVFASIAGGLKVNDAGTDLALALAIASNLLSIPMPKQSLV
                     IGELGLSGEVRSVNLLEWRIKEAMRLGFGEILIPASGKIPKISNEIKITKVAKIQEAV
                     DWLKNSVN"
BASE COUNT          632 a          327 c          388 g          509 t
ORIGIN      
        1 ccttacgtga cgtaagcttc cttaattcag atcttaaggg tgcacgcttt aatgctgata
       61 caaaaatgga aaacgtagat ttttctggag cagatctcac tggcgccaag attgagaaat
      121 tcacagcaaa gaaagtaatc tatgatgcaa agactaaatt tcctagcggt tttgaaccac
      181 gtcaatatgg tttcacaaga catggagaaa aagctgcgga aattgtaatc aaacgcgatc
      241 caaccgttag aaattcaacc gttactgatg acgcaatgcc taagaagaga aagaaaagaa
      301 aaaaaagtgc tgagtaaaat taagccttta aaaacagcct tcgagtagaa ggctgttttt
      361 tttgtcaatt tttaacaatt actttttcta atttaggatt atggcaaaaa ataaaacaac
      421 gatcgaatac gtctgtaaag aatgctcttc aacattttct aaatggtcag gcaaatgcag
      481 tgcatgcggt gcttggagta ctctagaaga acgtatcata gaagataaag ccaataaaag
      541 aggtcttgga ggccaagatt tagaaagttc atctcagacc cttaagagta ttcaaagtca
      601 agaaaaccaa aggttaagca cagggaattc agaatttgac cgaactcttg gtggagggct
      661 tgctccaggg tctttaattt taatcggtgg agatccaggc ataggaaaat cgactcttgt
      721 tttacaaact cttgccacca tgtctgctgc aaatgtaaaa acgctttacg ttagtggtga
      781 agaaagtgct gttcaagtta aaattagaag tgaacgatta gagctttctg gaagcgatat
      841 gcttttaatg tgtgaaacca atttggaaaa agtgttagca gaagcaaaac gtatccaacc
      901 agaaatttta gtcattgatt caattcaaac cgtttataaa acagaactac ctggtacgcc
      961 aggaagcgtg acacaactac gagaatgtac actcgagcta atggtttacg caaaaaacac
     1021 tgggtgtatc acgattcttg tggggcacgt aacaaaagat ggtcaaatcg ctggaccaag
     1081 aattttagaa catatggtcg atacggtagc gtattttgaa ggagatcgaa atcatcaata
     1141 tcgcgtactt agaacgataa aaaatcgatt tggggctacc gatgaaatcg gtgtttttga
     1201 aatgacttct agaggattag aagccgtgga gaatccaagt aaagtgtttt tacaaaatag
     1261 cgaagagcct ttacctggaa gtgttattag ttgcacttta gaaggcacta gagctcttct
     1321 ttttgaaacc caagctcttg tatcacaaac gaattatgcg gttcctcaac gtgtcgctgc
     1381 aggcattgat ccaaaacgtc taacgattat tcttgcccta ctagaaaaat tcgcatccat
     1441 tcgtattgga tcatcagatg tatttgcatc tattgcaggc ggccttaaag taaatgatgc
     1501 aggaacagat ttagcactcg cattagcaat tgcaagcaat ttattaagta ttcctatgcc
     1561 taaacaaagt cttgtgattg gagaactggg gctttcagga gaagttcgat ccgtaaatct
     1621 tttagaatgg cgaattaaag aagccatgcg tcttggtttt ggggaaatat taatccctgc
     1681 ctcaggtaag atcccgaaaa tttctaatga gattaagatt acaaaagtgg ctaaaattca
     1741 agaagccgtt gactggctaa aaaactccgt aaattaaatt aataaaatca ttttttgata
     1801 caaaaaagaa acccaccggc taagccggtg gttcttcatt tacgggcata gcccta
//
LOCUS       DAOOBB010000262         1734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_75489_length_1734_cov_2.706968, whole genome shotgun sequence.
ACCESSION   DAOOBB010000262 DAOOBB010000000
VERSION     DAOOBB010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75489_length_1734_cov_2.706968"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1734)
                     /locus_tag="PLT31_09065"
     CDS             complement(<1..>1734)
                     /locus_tag="PLT31_09065"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HPW95320.1"
                     /translation="VHMAKKALKQKSNPMKAVKKSTSKKYGYFDDANKEYVLTNPATP
                     IKWCNYVGTLKFGGIVDTTGGSLICKGDPALNRITKYIAQMPGSDFKASTVYIRIKDG
                     KNYKVFSPFVTPTLIPMDKWECHVGLSYSRWIAEAFGLRIQITIFVPTGSNTLLQDIQ
                     VTNIAGGAKEVDIIPVYEFSHFEAEKQLTNADWVPQTMTLKAHFEKTGHCVLEQYAYM
                     KRDLAVNYVTADAPIASFDGDRRVFLGANEMGSWANPLSLQNKELSNSECDRGDNIAA
                     FMIHAGKLGAKKSFRTCTQLGQEDSLKIATKAIKKYRDLKNVDKAFDELAKFWENYLA
                     TVQVNTPDASFNSMVNVHNPRQCHTTKNWSRYLSLYQLGYGTSRGIGYRDSSQDLLGV
                     MAHMPEEALELAKNLLSVQRPEGNAMHQYLPSKLDRDEGDEANEGDSREKAADGYCNW
                     YGDDHLWIVLTVAGYLKETGNLDFLKEEIPYYQKGKKRAERVKGSVLEHLQKSLKFSR
                     EHLGKHNLPLLGFADWNDCMNLAPGAESIFNASLYARALLEMMDICEVIGDKKSVEMY
                     KTWYNDVKNALN"
BASE COUNT          500 a          359 c          341 g          534 t
ORIGIN      
        1 gttaagcgcg tttttaacgt cgttatacca tgtcttgtac atttcaacag attttttgtc
       61 tccaatgact tcacaaatat ccatcatttc tagaagagct ctagcataaa gactggcgtt
      121 gaaaatagat tctgcaccag gagcaaggtt catgcagtca ttccagtctg caaaaccaag
      181 aagtggtaga ttatgcttac ctaaatgttc tcttgagaac ttcaagctct tttgtaagtg
      241 ttccaagaca gaacccttaa cacgttcagc acgtttttta cccttctggt aataaggaat
      301 ttcttctttg aggaaatcaa gattgcccgt ttcttttaga taaccagcga ccgttaatac
      361 gatccaaaga tggtcatccc cataccagtt gcaatagcca tcggcagcct tttcgcggct
      421 atcgccttca ttagcttcat cgccttcgtc gcgatctaat tttgatggca agtactggtg
      481 catggcattg ccttctggac gttgtacaga aagaaggttc ttagcaagtt caagagcttc
      541 ttctggcata tgagccatta cacctaaaag gtcttggcta gagtcacgat aaccaatacc
      601 acgagaagta ccataaccta attgatacaa ggaaaggtaa cggctccagt tcttggtggt
      661 atggcattga cgtggattat gcacgttcac catagaattg aaagaagcat ctggagtatt
      721 tacttgtact gtggccaagt agttttccca gaatttagca agctcatcaa aagccttatc
      781 gacatttttt aggtcacgat atttcttaat cgctttggtg gcgattttaa gagaatcctc
      841 ctgaccgagt tgagtacaag tacggaagct ctttttagcg cctaattttc cagcatgaat
      901 cataaaggcg gcaatgttat cgccacgatc acattcgcta ttagaaagct ctttgttttg
      961 aagagaaaga ggattagccc agcttcccat ttcatttgcg ccaaggaata ctcgacgatc
     1021 gccatcaaag cttgcaatag gagcgtctgc tgttacatag ttcaccgcta agtcgcgctt
     1081 catataagcg tattgttcta aaacacagtg acctgttttt tcgaagtgag cttttaaggt
     1141 cattgtttga ggaacccagt cagcattggt caattgcttt tcagcttcaa aatggctgaa
     1201 ttcataaaca ggaataatgt cgacttcttt agcgccacca gcaatgtttg tgacctggat
     1261 atcttgaagt aaagtgtttg atccagtagg aacaaaaatc gtaatttgaa tgcgtaagcc
     1321 aaaagcttct gcaatccaac ggctgtaaga aaggccaaca tggcattccc atttatccat
     1381 tggaattaaa gtaggcgtta caaatggaga aaatacttta tagttttttc cgtctttaat
     1441 acgaatataa actgtgcttg ccttaaaatc agaaccaggc atttgagcaa tgtactttgt
     1501 gattcgattt aaagcaggat cacccttaca aatcaaagaa ccaccagtag tatcgacgat
     1561 accaccaaat tttaaagtac caacatagtt gcaccatttg attggggttg caggattagt
     1621 gagaacatat tccttatttg catcatcaaa atacccgtat ttttttgagg tacttttctt
     1681 aactgctttc atagggttag acttttgctt taacgctttt ttagccatgt ggac
//
LOCUS       DAOOBB010000263         1692 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_78152_length_1692_cov_2.674404, whole genome shotgun sequence.
ACCESSION   DAOOBB010000263 DAOOBB010000000
VERSION     DAOOBB010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78152_length_1692_cov_2.674404"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PLT31_09070"
     CDS             complement(<1..345)
                     /locus_tag="PLT31_09070"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPW95321.1"
                     /translation="MKKGFTLIELMVYVALLGVIIVVAGRVYSDSTKFRLRTQNMLKA
                     TEEANRAASLIQEDLSSMGSKSWKVLGAGIDSFYVETSVYLDPDNVTEANKDSSSYVL
                     TRSGDGKDSILFR"
     gene            complement(342..512)
                     /locus_tag="PLT31_09075"
     CDS             complement(342..512)
                     /locus_tag="PLT31_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95322.1"
                     /translation="MNYNVEINVSDDATYASEEKSYYGSMDHTYAKRLDVKVSWPFKG
                     TTHSINYSGVIR"
     gene            complement(525..809)
                     /locus_tag="PLT31_09080"
     CDS             complement(525..809)
                     /locus_tag="PLT31_09080"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPW95323.1"
                     /translation="MGNSIKKQNRCRGFGIIEILISIVVLGFLMTALLNLQLGNRDAL
                     LRIRARDGAVDVAQDAIDSLSILGVSALSKTALPSQKISFSKTRSWQRTG"
     gene            complement(794..1351)
                     /locus_tag="PLT31_09085"
     CDS             complement(794..1351)
                     /locus_tag="PLT31_09085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPW95324.1"
                     /translation="MEKRSHYTSGFTLIEVLVVVAIIGILSGAGISSLNGAIANNRLK
                     DAIVNTRAFVEVMSEESKRLNDSLCIQFEPRKMTTYRFANAVCGGTVVDSKSVDDPVS
                     FFASSIGSVTTAETFLGTPAIWSSTGTYFILSPEQGLNSVSGEGIIVLRYGNTDRYGA
                     VIKTGKKNRWQSFFSVDGGTSWVTP"
BASE COUNT          572 a          349 c          284 g          487 t
ORIGIN      
        1 tctaaagaga atagaatctt tcccatctcc tgaacgagtg agtacatagg aagacgaatc
       61 tttatttgct tcagtcacat tatcgggatc taaataaaca ctagtttcta cataaaatga
      121 atcaatacca gcccctagta ctttccagga ctttgaaccc atagaagaca agtcttcttg
      181 aattagagaa gcggctctat ttgcttcttc tgtggctttt aacatgtttt gcgtgcgaag
      241 ccgaaattta gtgctatcac tataaacacg gccagcaaca acgataataa cgcctaacaa
      301 agcaacataa accatcaatt ctataagtgt aaaacccttt ttcatcggat tacccccgag
      361 taattaatgg agtgagtagt ccctttaaaa ggccaactga ctttgacatc gaggcgttta
      421 gcgtatgtat gatccataga cccataatat gatttttctt cagaagcata agtagcatca
      481 tctgaaacat tgatttcgac attgtagttc actttaatag ggaatcaacc tgtacgctgc
      541 caggatctgg tttttgaaaa actgattttt tggctaggga gtgctgtttt tgataaagcc
      601 gaaacaccta atatagagag agaatcaatc gcatcttgag ccacatccac ggcgccatcg
      661 cgagctcgta tgcgtaataa agcatctcga ttgccaagct gtaaattcaa taaagccgtc
      721 atcaaaaagc ctaataccac aatggatatt agaatttcaa taataccaaa accacgacaa
      781 cggttttgct tttttatgga gttacccatg aagtacctcc atcgactgaa aagaaggatt
      841 gccaacgatt ttttttacct gttttaataa ccgcaccata acgatctgta tttccataac
      901 gtaaaacaat aatcccttct ccactcacac tatttaagcc ctgttctgga gataaaataa
      961 agtacgttcc cgttgaactc caaatagcag gagttcctaa aaaagtttct gccgtagtca
     1021 cgctccctat tgaagaagca aaaaaagaaa cgggatcatc cactgattta gaatccacta
     1081 cggtaccgcc acatactgcg tttgcaaaac ggtaagtggt catttttctt ggttcaaatt
     1141 gaatacaaag agaatcattt aagcgtttag attcttctga catcacttct acaaaagcac
     1201 gcgtattgac aatagcgtct tttagtcgat tattagcaat ggcaccattc aaagaagaga
     1261 ttcctgcacc agagagaatc cctatgatag cgactaccac taatacttcg attagtgtaa
     1321 aacctgatgt ataatgactt ctcttttcca taaaaaccac actaaataca agaaaataaa
     1381 agacttaaga gcctttaaga gtgacattat aaatatatat tatttgatgt aaattttttc
     1441 tttacttctt attttgttct tgcaaatcaa tctcgaacaa catggattca caaatgaagc
     1501 aaaaaccctt gtgggctatc acaagaaata aaacgactca cgtttttttg atttttagtg
     1561 cgagcaatat aaactgaagt aaaaaagctg tgataatcgt cactgaaagg ctaatgagag
     1621 gcatccaagg ccagcttaat atgcctgata aagcaccttc tattcccata aagggaatac
     1681 cattgaacaa ag
//
LOCUS       DAOOBB010000264         1649 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_80947_length_1649_cov_4.483689, whole genome shotgun sequence.
ACCESSION   DAOOBB010000264 DAOOBB010000000
VERSION     DAOOBB010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80947_length_1649_cov_4.483689"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="PLT31_09090"
     CDS             <1..890
                     /locus_tag="PLT31_09090"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HPW95325.1"
                     /translation="ERRKKYLWILGKGKTIPVYLIQIQKHIQENQGHILNMEELFLPN
                     TFQAASLIFTDRLGDTISIELQISENIFSDNISKIGVAFEVETLSSELITKLNELDVS
                     YSILITPFNLPDPFYSQIAQLQNAEPVLWLYMESEKLRSSHHQKSPIRIHHTEEKIEE
                     VIEMAKKKLPNAKGVTTRYAEKAVEHRNLLNAIFKSIQQKNLWFLDLTENRSSLVNEI
                     CKNYSIKCKQISAYSEKTSTVEDYISKNLRLALRTGTSYIILPLTSKNIAEIEKIKEK
                     AASQGTEITTLSNVIAVTK"
     gene            899..>1649
                     /locus_tag="PLT31_09095"
     CDS             899..>1649
                     /locus_tag="PLT31_09095"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014545445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HPW95326.1"
                     /translation="MTVKLGVNIDHIATIREARKIREPDPVAAAILAELAGCHGITAH
                     LREDKRHIQDRDIRLLRGIVTTHLNMEMAPTQEMVQFAIDVQPDMVTLVPENRQEITT
                     EGGLNVAAKTDELARFIMTLKNNDIAVSVFIDPDTDQVKASRKVGADFVEFHTGKFSA
                     AYEMGSAQDLNREVSAIQDMTVLASKYGLRANAGHGLNYRNVGYIAAIEGIEELNIGH
                     SIIGRASLVGMEKAVKEMLEANPHYNQFKAFQ"
BASE COUNT          586 a          306 c          295 g          462 t
ORIGIN      
        1 ctgagaggcg caaaaaatat ttatggattc tcgggaaggg aaaaacaatc ccagtctatt
       61 tgatacaaat tcaaaaacac attcaagaaa atcaaggtca tatattaaac atggaagaat
      121 tatttcttcc taacacattt caagcagcct ctctaatttt tacagaccgc ctaggagata
      181 cgatttctat cgagcttcaa atatcagaga atattttttc tgataacatt tcaaagatag
      241 gagttgcttt tgaagtagaa actttatcta gtgaactaat taccaaatta aatgaattag
      301 atgtttccta ctctatttta attaccccct ttaacttacc tgatccattc tattctcaaa
      361 tcgctcaact acagaacgca gagcccgttt tatggcttta tatggaatct gaaaaactta
      421 gaagttccca tcatcaaaaa agtccaatcc gaattcatca taccgaagaa aaaatagaag
      481 aagtgataga aatggcaaag aaaaaacttc ctaatgccaa aggagtcact acaaggtatg
      541 cggaaaaagc agtcgaacac aggaatttat taaacgctat ttttaaatct attcaacaaa
      601 aaaatctttg gtttttagat cttactgaaa atcgttcttc tttagtgaat gaaatttgca
      661 aaaactattc tataaaatgc aagcagatta gtgcttattc tgaaaaaact agtacggttg
      721 aagattacat ctcaaaaaat cttcgtctcg ccttaagaac aggaacctca tacatcattt
      781 tacctttaac ttcaaaaaac atcgctgaaa ttgaaaaaat aaaagaaaaa gcagcctctc
      841 aagggacaga aataactacg ttatccaatg tcattgctgt tacaaaatag gagtacctat
      901 gaccgttaaa ttaggtgtca acatagatca tatcgccacc attcgcgaag ctcgtaaaat
      961 aagggaacct gatcctgtag ccgccgctat tcttgctgaa ttagcaggtt gtcatggtat
     1021 cacggctcat ttgcgcgaag ataaacgtca catccaagat cgagacattc gattattacg
     1081 aggcattgta acaacgcatt tgaatatgga aatggctcct actcaagaaa tggtgcaatt
     1141 tgcaatcgat gtgcaaccag atatggtgac tttagttcct gaaaacagac aagaaatcac
     1201 tacagaaggc ggcttgaatg tagcagctaa aacagatgaa ctagcccgct ttattatgac
     1261 tcttaaaaac aatgatattg ccgtcagtgt ttttattgat ccagatacag atcaagtgaa
     1321 agcctctaga aaggttggag ctgattttgt ggaattccac actggtaagt ttagtgccgc
     1381 ttatgaaatg ggtagtgctc aagatcttaa tcgagaagtt tcagccatac aagatatgac
     1441 tgttcttgca agcaaatacg gactacgtgc caatgcgggc catggattaa attatcgaaa
     1501 tgtaggctat attgcagcca ttgaaggcat tgaagaatta aacatcggcc atagtattat
     1561 tggacgggcg tccctagttg gaatggaaaa agcagtgaaa gaaatgcttg aagccaatcc
     1621 acattataat caattcaaag cttttcagc
//
LOCUS       DAOOBB010000265         1645 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_81226_length_1645_cov_2.902516, whole genome shotgun sequence.
ACCESSION   DAOOBB010000265 DAOOBB010000000
VERSION     DAOOBB010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81226_length_1645_cov_2.902516"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="PLT31_09100"
     CDS             <1..1002
                     /locus_tag="PLT31_09100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPW95327.1"
                     /translation="FRYAKPSLEILTHDEGAPSKMANKPWMQLLAIPYIISGFIKCLQ
                     LCRQEKFDLLHVHWPFPHGLIALGASKLFKIPMLLNFHGAELLLIRKHAWVAPVLRFI
                     LSQSQAVFANSHFTAQKINALRKTEVYCSPYGTTLSSQVSTELHPVKNKFRILFVGRH
                     IERKGLIYLIEAASKLDPSRFEVRIAGAGDLTSSLMEKAKEIAPETVVFTGKLSSKEL
                     QEEYQKANVFVLPAIVDQKGDTEGLGVVLIEAIEYALPVVASNVGGIPDVIIDHETGL
                     LVEAKNSDALTDAFLKLESDEALSQKLVTGARAHIRKYFSWEPIIKNQIEVYKSVLK"
     gene            complement(1009..>1645)
                     /locus_tag="PLT31_09105"
     CDS             complement(1009..>1645)
                     /locus_tag="PLT31_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95328.1"
                     /translation="SGLIIILAPPANAAPAMAKILGGNSILALKIFISGHVIACISIP
                     LLFGYFTNTDESFIGMSKQIFGSIQPIITIPLSIAFGLRAFYPEIARRIVSFQKYTLV
                     VWMVSVFIIIAKASYDIREMGFMSLWESGMFPLLGGLSLFLCVFLFFMGWLSSRKKHP
                     IEGAQSMGQKNTTLIIWITQMYAGPIAAIGPVFYVIWQNLVLSYMSRGKKK"
BASE COUNT          516 a          345 c          318 g          466 t
ORIGIN      
        1 tttcgctatg ccaaaccctc tttagaaatt ttaacacatg atgaaggggc tccttctaaa
       61 atggctaaca agccctggat gcagttgctt gcgattccct atattattag tggttttata
      121 aaatgcttgc agttgtgtcg ccaagaaaag tttgatcttc tccatgttca ctggccattc
      181 cctcatggtt taattgcact tggtgcttct aagcttttta aaatcccgat gcttttgaac
      241 tttcacggcg ccgagcttct tttaattaga aagcatgctt gggtcgctcc tgtccttcgc
      301 tttatcttaa gccaaagtca agccgttttt gcaaacagtc attttaccgc tcaaaaaata
      361 aatgcccttc gaaaaacaga ggtgtattgt agcccttatg gaacgacttt atcttctcaa
      421 gtaagtacag aattgcatcc tgtaaaaaat aaatttcgca ttttatttgt aggccgtcac
      481 attgagcgaa aaggtctaat ttatttaata gaagcagcct caaaattaga tccttcgcgt
      541 tttgaagtac gtattgcagg tgctggcgac ttgacctctt ctttaatgga aaaagcaaaa
      601 gaaatcgctc ccgagacggt tgtcttcaca gggaaacttt cttccaaaga actacaagaa
      661 gaatatcaaa aagcgaatgt ctttgttttg cccgcgatcg tcgatcagaa gggcgataca
      721 gaaggcttag gcgttgtgct gattgaagcc atagaatatg cccttcctgt tgttgcctct
      781 aatgttggcg gcattcccga tgtgattatc gatcatgaaa caggccttct tgtagaagct
      841 aaaaatagcg acgctcttac cgatgccttc ttaaagctcg aatccgacga agctttgtct
      901 caaaagctag ttactggtgc gcgagcccat atcagaaaat acttctcctg ggaacccatc
      961 attaaaaacc aaattgaagt ctataaatcc gttcttaaat aaaacgattt atttcttttt
     1021 gcctcggctc atgtaactta aaactaagtt ttgccaaatg acataaaaca caggcccaat
     1081 ggcggctatt ggcccagcat acatttgagt gatccaaata attagcgtgg tatttttttg
     1141 ccccatgctt tgtgcccctt caataggatg tttttttctc gaagaaagcc agcccatgaa
     1201 gaaaagaaat acgcataaaa ataaagagag cccacccaat agagggaaca taccgctttc
     1261 ccataaagac ataaagccca tttcacgaat atcgtagcta gcttttgcaa taatgataaa
     1321 gacagaaacc atccaaacca ctaaagtata tttttgaaaa gaaacaatcc gtctagcgat
     1381 ctcagggtaa aaagcgcgga ggccaaaggc aatcgaaaga ggaatcgtga tgattggttg
     1441 aatagaacca aagatctgct tagacatgcc tataaagctt tcatccgtat ttgtgaaata
     1501 gccaaatagt aaaggaatcg aaatacaagc aatcacgtga ccagaaataa atattttcaa
     1561 agctaagatc gaatttccac caaggatttt agccatcgct ggagcggcat tggctggagg
     1621 agctaaaatg ataatcaaac ctgaa
//
LOCUS       DAOOBB010000266         1638 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_81675_length_1638_cov_5.583070, whole genome shotgun sequence.
ACCESSION   DAOOBB010000266 DAOOBB010000000
VERSION     DAOOBB010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81675_length_1638_cov_5.583070"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(271..795)
                     /locus_tag="PLT31_09110"
     CDS             complement(271..795)
                     /locus_tag="PLT31_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HPW95329.1"
                     /translation="MKYFSLTALLVAILAFMAVAEEPPPRGGVGKVTIITDPPNSQVF
                     LGGEDLGKSPIVEREFKSGRHTLVVIDQGFELINERFNVWPNKVNTFGGKTVIPKGHI
                     RITTTPAKCIIYVDGEHSDQTGGAALTVRNLDAGDHVVRAECSNRKSVEELVKVEGEK
                     TIEVHLDATGKKKK"
     gene            1028..>1638
                     /locus_tag="PLT31_09115"
     CDS             1028..>1638
                     /locus_tag="PLT31_09115"
                     /inference="COORDINATES: protein motif:HMM:NF021347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-ATPase domain-containing protein"
                     /protein_id="HPW95330.1"
                     /translation="MKELYRKLLSLTGKKYGLYKSLSQKTWNLGDFDLEFIHIQGDPY
                     APASKILLTAPFSCLGLQPQFINSFTKRLATSDFLLRRLSQAIEEWNPKDQPVFTVMK
                     PGQEVVLRNALWIENGKVQVCLQIKLPGNQRIIDASEAANLITSVLPDLLTSSLYDFG
                     GQFLETLKKHIECLEIREALKEQIQSSGLIAFIPNGAILPRASG"
BASE COUNT          506 a          321 c          265 g          546 t
ORIGIN      
        1 ttcattttga atagagtcgt tacttctata gaatccatga ctccataaat atcttgatag
       61 tatgccgtcc atgtgactgt actgtctttt tcaaaaggtt tgtttaagat agcaaagatg
      121 taagatcgat aatattttgg tgtcaatgga tacttaatgt atgtcgtatc ataacaagga
      181 ttaagagtct ttaaaaattc taagctattt taatgccata aaaaaagacc tttttgaaaa
      241 aggcctttta aaatttcatt agtagaataa ttacttcttc tttttaccag tggcatctaa
      301 atgcacttca attgtctttt caccttctac tttcactaat tcttcgacgc tcttgcggtt
      361 actgcactca gcgcgaacaa cgtggtcacc cgcatcaaga ttacgaacag ttaaagcagc
      421 tccacctgtt tggtcagaat gttcaccatc aacatagatg atacatttag ctggagtggt
      481 agtgatgcga atatggcctt ttggaatgac cgttttgcca ccaaaagtat tcactttatt
      541 tggccaaaca ttgaaacgtt cattgattaa ttcaaagccc tgatcaatca caactagagt
      601 atgacgtcca gatttaaatt cacgctcgac aattggactt tttccaagat cttcaccacc
      661 caagaatacc tgactattag gagggtctgt gataatggtt actttaccga caccgccacg
      721 aggaggtggt tcttctgcaa ctgccatgaa agctaaaata gcgactaaca gagctgtaag
      781 agaaaaatac ttcattaaaa aactcctaag atcaaaagat ttacctctaa aatataaaga
      841 ttttgcttag aaagtggcat ttttttttaa ttaggctcaa aacgagccgt tttcatgtaa
      901 ctttttttta agtctttctt ttttgctctt atttgcccat gattattttt attccttctt
      961 aaaatctttt ttacgtcgct taaaaaacaa actattattt gtaattcaat cgcttaaagg
     1021 attttgaatg aaagaattat atagaaagtt gctttcttta actggcaaaa aatacgggtt
     1081 atataaaagt ttatctcaaa aaacctggaa tttaggcgat ttcgatttag aattcattca
     1141 tattcaagga gatccctatg ctcctgcctc taaaattctt ttaaccgctc ctttctcttg
     1201 cctaggctta caaccacagt ttattaattc atttacaaag cgcctcgcga cctctgactt
     1261 tttacttcgt cgattgtctc aagccatcga agaatggaat cccaaagacc aacccgtttt
     1321 cacagtgatg aaaccaggtc aagaagttgt tttgcgtaac gctctctgga ttgaaaatgg
     1381 taaagtacaa gtctgtttgc aaataaaact acctggaaat cagcgaatca ttgatgcaag
     1441 tgaagctgct aatttaataa cctccgttct cccagactta ctcacttcta gcctttatga
     1501 ttttggaggc cagtttttag agactcttaa aaaacatatt gaatgtttag aaattcgcga
     1561 agccttaaaa gaacaaatac aatcctctgg ccttattgct tttattccta atggcgccat
     1621 tttaccaaga gcctctgg
//
LOCUS       DAOOBB010000267         1594 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_84826_length_1594_cov_4.170890, whole genome shotgun sequence.
ACCESSION   DAOOBB010000267 DAOOBB010000000
VERSION     DAOOBB010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84826_length_1594_cov_4.170890"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..894
                     /locus_tag="PLT31_09120"
     CDS             163..894
                     /locus_tag="PLT31_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPW95331.1"
                     /translation="MLYGIFSDIHSNEAALTAVLASMHDLGVERRICLGDLVGYGVDV
                     NECVSLTKENADVCLIGNHDSVAIRWESSVGFNPYAKKMIEWTQEAITEESASYIKSL
                     PYMFEENDICFVHASPMSPADWIYVTDLEDALDAFDHFSGSFCFVGHTHSPIIVSMRG
                     GGAIPKVIEESAYKIEGTERLLVNVGSVGQPRDRDPRASWCLFDSTQKKVKIVRVEYD
                     IAQTQARMRAFSAPDFLVERLGVGR"
     gene            891..1349
                     /locus_tag="PLT31_09125"
     CDS             891..1349
                     /locus_tag="PLT31_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HPW95332.1"
                     /translation="MKWTLCVFGKAGSPFVADEVAKYSKRLRSYISFDIVELKESKRD
                     DKTRGLAEEAADFEKKFPKSDYAWIILAEEGKLLNTVQLAKWTEQKASTPCVFLIGSA
                     YGISSSIKEKADLLLSLSPLTLTHDHARVILAEQLYRCEMVHRNHPYHHQ"
     gene            complement(1448..>1594)
                     /locus_tag="PLT31_09130"
     CDS             complement(1448..>1594)
                     /locus_tag="PLT31_09130"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HPW95333.1"
                     /translation="FKEEAAAQAIEKAVEKVIAQGYRTGEIWTEGCQKVGTSGMGDAI
                     IAAL"
BASE COUNT          444 a          279 c          342 g          529 t
ORIGIN      
        1 cacaggtcag gatgctttta gaagataaaa gttctgttcc agacccctat tatggcactg
       61 aaaaggcttt ttacgacact tttgacatta tttatagggg tattgagtta tttttacgag
      121 atttattgta ataaatgaat ttcgtttttt tatattcctc ttatgcttta tggaatcttt
      181 tcagatattc attctaatga agctgcttta actgctgttc ttgcatcaat gcatgatctg
      241 ggagtggaac gccgcatttg tttaggtgat ttagttggtt atggtgtgga tgttaatgaa
      301 tgtgtttcat tgactaaaga aaacgccgac gtttgtctta ttggtaatca cgatagcgta
      361 gctattcgtt gggagtcgtc tgtagggttt aatccttacg ccaaaaagat gattgagtgg
      421 actcaagagg ctataacgga agagtctgct tcttatatta aatcattgcc ttatatgttt
      481 gaggaaaatg atatttgttt tgtacatgca tcaccaatgt ctccagccga ttggatttat
      541 gtaacagatc tagaagacgc cttagatgct tttgatcatt tttcagggtc tttttgcttt
      601 gtgggccata ctcattctcc aatcattgtc tccatgagag gtggtggagc tattccaaaa
      661 gtgattgaag aaagtgcata taaaattgag ggtacagaac gcttgttggt gaatgtgggg
      721 agcgttgggc aacctcgaga tagagatcct agggcttctt ggtgcttatt tgactccact
      781 caaaaaaagg tgaaaattgt acgtgtggag tatgatattg cacaaacgca agctcgaatg
      841 agagctttct ctgcacctga ttttcttgtg gagcgacttg gagtgggccg ttgaagtgga
      901 cgctttgtgt ttttggtaag gcgggctctc catttgttgc cgatgaagtt gcaaagtaca
      961 gtaaaagact tcgttcttat atttcttttg atatagttga actcaaagaa tcgaagcgag
     1021 acgataaaac gcgcggtctt gctgaagaag ccgctgattt tgaaaaaaaa ttccctaaat
     1081 ccgattacgc atggattatt cttgccgaag agggaaaact tctgaataca gtacaattag
     1141 ccaagtggac agaacaaaaa gcttcaactc cttgtgtgtt tttaattggt tcggcttatg
     1201 gtatttcgtc ttccattaaa gaaaaagcag atctactttt atcgctttcg cctttgactc
     1261 tcactcatga tcatgctcgt gttattttag cggagcaact gtatcgatgc gagatggtgc
     1321 atcgaaatca tccataccat catcaataaa aattgtaaat aaaaaacgaa cttaaaataa
     1381 aaaagcgagc taaggtgttt tccaaagctc gcttttcatc aatatttttt tgaaagaatt
     1441 ttaattctta aagagctgca ataatagcgt cacccatacc agaggtacct actttttggc
     1501 aaccttcggt ccagatttca ccagtacgat acccttgagc aatgactttt tctacagcct
     1561 tttcaatcgc ttgagcagcg gcttcttctt tgaa
//
LOCUS       DAOOBB010000268         1520 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fibrobacteraceae bacterium isolate AMR_MDS_4055
            NODE_90630_length_1520_cov_3.656655, whole genome shotgun sequence.
ACCESSION   DAOOBB010000268 DAOOBB010000000
VERSION     DAOOBB010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866026
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fibrobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Fibrobacteraceae bacterium
            Bacteria; Fibrobacterota; Fibrobacteria; Fibrobacterales;
            Fibrobacteraceae.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 07:07:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,828
            CDSs (total)                      :: 1,792
            Genes (coding)                    :: 1,790
            CDSs (with protein)               :: 1,790
            Genes (RNA)                       :: 36
            tRNAs                             :: 35
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Fibrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90630_length_1520_cov_3.656655"
                     /isolate="AMR_MDS_4055"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2652288"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /gene="ispG"
                     /locus_tag="PLT31_09135"
     CDS             complement(<1..971)
                     /gene="ispG"
                     /locus_tag="PLT31_09135"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HPW95334.1"
                     /translation="MHKKTGLTYMASRFHAIRRDTIKVRVGNTWIGGDAPILVQSMTT
                     TKPKDLEATVSQVLALANASCKLVRITVPTFSDAMALEEVVKRVRAAGCDVPISADIH
                     FQPKAAFEALKWVDKVRINPGNFVDSVTDAEDFEQGKQKVYDSFAPFLAEAKRYGRAI
                     RIGVNHGSLSARMLYKYGDTVEGMVESALEYLSVCEKEDFDQVVVSLKSSNPKVAIQA
                     YRLLAQRLKEEKFKPYPFHVGVTEAGEGEDGRLKSAVGIGALLLDGLGDTIRVSLTED
                     AVAEIPVALDLIEACTIVNIPKIDVPELEKDPFHYARRETASVCFAGA"
     gene            complement(1072..>1520)
                     /locus_tag="PLT31_09140"
     CDS             complement(1072..>1520)
                     /locus_tag="PLT31_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPW95335.1"
                     /translation="FKDISPDLNARAIYKRYTEETHDEFNFSEYDIVIDAIDSLSSKI
                     DLLVQAYQAGCTVYSSMGAAWKLDPTQVRVASIWQTKGCPLGKLVRTGLRERGFTGDF
                     KAVFSEEKRPVLSEEKAIKSPSVLGSAMPVTSMFGATLASQVLQNI"
BASE COUNT          472 a          327 c          267 g          454 t
ORIGIN      
        1 gcgcctgcaa aacaaacaga agcggtttca cgacgggcat aatggaatgg gtctttttct
       61 aattcgggaa catcaatttt tggaatattg acaatagtac aagcttcaat taaatcaaga
      121 gcaactggaa tttcagctac ggcatcttct gttaaagaca ctctaatggt gtctccaaga
      181 ccgtctaata gaagagctcc aatacctact gccgatttca agcggccgtc ttcaccttcg
      241 ccagcttctg tgacacccac atggaaagga tagggtttga atttttcttc ttttaatctt
      301 tgagctaaaa gacgataggc ttgaatagcc accttaggat ttgagctttt taagctaacc
      361 acgacttggt caaaatcttc tttttcacaa acagataaat attctaaggc actttctacc
      421 atgccttcca cggtgtctcc atatttataa agcattctcg cagataacga accgtgattc
      481 actccaatac gaatggctcg gccataacgt ttcgcttcgg ctaaaaatgg agcaaaggaa
      541 tcataaacct tctgttttcc ttgttcaaaa tcttctgcat cagtcacaga gtccacaaaa
      601 tttcctggat tgatacgtac tttatcgacc cattttaaag cttcaaaagc agcttttggt
      661 tggaaatgaa tatcagcaga aataggtaca tcacaaccag cagcacgaac gcgtttaacg
      721 acttcttcta aggccattgc atcggaaaaa gtagggactg taattctaac taatttacat
      781 gaagcattgg ctaaagctaa aacttggctt actgttgctt ctagatcttt gggttttgtg
      841 gtcgtcatgg attgtacaag aattggtgca tcaccaccga tccaagtatt ccctacacgc
      901 acttttattg tatctcgacg tatcgcatga aagcgactcg ccatatacgt taatcctgtt
      961 tttttatgca tacggcaaat atagaaagaa tagagaattt cggcgatttt tataaaaatc
     1021 caaattcatt aaaaacgcct aaatggctaa aaaatgcaaa aaaagcatta cttaaatatt
     1081 ttgtaatact tgacttgcta aagtagcacc aaacatggac gttacgggca tggcactacc
     1141 caatacagaa ggagatttga ttgctttttc ttctgataaa acgggacgct tttcttcact
     1201 aaatacagcc ttaaaatctc cagtaaagcc tctctctcga agtcctgtcc gaactaactt
     1261 accaagagga cacccttttg tttgccaaat actagcaaca cgcacttgag tggggtctag
     1321 tttccaggct gctcccatac tagaataaac agtacaaccc gcttgatatg cttgtactag
     1381 tagatctatt ttcgaagata aagaatcaat ggcatcgatg acaatatcgt attcactaaa
     1441 attaaattca tcatgcgttt cttccgtata ccttttatat atggctctcg catttaaatc
     1501 tggagagata tctttaaaac
//
